LOC105208490 gene cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

mRNA	Protein	Name
XM_011178360.1	XP_011176662.1	uncharacterized protein LOC105208490 isoform X1
XM_011178362.1	XP_011176664.1	uncharacterized protein LOC105208490 isoform X1
XM_011178365.1	XP_011176667.1	uncharacterized protein LOC105208490 isoform X4
XM_029037938.1	XP_028893771.1	uncharacterized protein LOC105208490 isoform X2
XM_011178367.2	XP_011176669.1	uncharacterized protein LOC105208490 isoform X4
XM_029037941.1	XP_028893774.1	uncharacterized protein LOC105208490 isoform X6
XM_011178367.1	XP_011176669.1	uncharacterized protein LOC105208490 isoform X6
XM_011178363.2	XP_011176665.1	uncharacterized protein LOC105208490 isoform X1
XM_011178364.1	XP_011176666.1	uncharacterized protein LOC105208490 isoform X3
XM_011178366.1	XP_011176668.1	uncharacterized protein LOC105208490 isoform X5
XM_029037940.1	XP_028893773.1	uncharacterized protein LOC105208490 isoform X5
XM_011178359.1	XP_011176661.1	uncharacterized protein LOC105208490 isoform X1
XM_011178363.1	XP_011176665.1	uncharacterized protein LOC105208490 isoform X2
XM_029037936.1	XP_028893769.1	uncharacterized protein LOC105208490 isoform X1
XM_029037939.1	XP_028893772.1	uncharacterized protein LOC105208490 isoform X3
XM_029037937.1	XP_028893770.1	uncharacterized protein LOC105208490 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa254489	XM_029037940.1 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa254490	XM_029037941.1 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa194182	XM_011178364.1 Latest version!	Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa254487	XM_029037938.1 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa254488	XM_029037939.1 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa194180	XM_011178362.1 Latest version!	Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OBa194180	XM_011178359.1 Latest version!	Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OBa194180	XM_011178360.1 Latest version!	Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OBa194181	XM_029037936.1 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa194181	XM_029037937.1 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa194181	XM_011178363.2 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa194183	XM_011178365.1 Latest version!	Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa194184	XM_011178366.1 Latest version!	Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa194185	XM_011178367.2 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
View Old Accession Versions >>
OBa194185	XM_011178367.1	Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa194181	XM_011178363.1	Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
Hide Old Accession Versions >>

LOC105208490 ( XM_029037940.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028893773.1 Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa254489
Clone ID Related Accession (Same CDS sequence)	XM_029037940.1
Accession Version	XM_029037940.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105208490 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863673.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GAAAAGCAGC AGCGGCGGCA GCAGCAGCAG CAACAACTCC 
ACAAAGCTGA ACACCCACAA GAGTGCTGGC GGTAAGGTGA GCCTACATGC AATTGTTGGC 
AGCGCTGGTG ACAACAACAC CAACAGCGGC AGCGCTAGTC GAATCAACAA CGACTTAACA 
GAGTCACCAA AAAGCACAAC AACAACAGCG AAAAGTTCGA ATGAAGCATT GAGTGGTACA 
AAAACGTGGC AGCAGCAGCA GCGGCGTAGC AACGCTCGTC ATTTGGAACA AGCTACTCTA 
TATGGCCAAA ACAGCAATTC ATCTAAAATG CGCGTAGGCG GCGCTGATTG TGATGATTGT 
TATGCGGTTG TGTCGGCTGA CGCAGATGAG TCGCAGCTGC GCTACCACCA ACACCACCTT 
CAACCGCAAC AGCTACGTAA ACAACAACAA CAGCAGCAAC AAAGCAGTGT TGTGCTAAGT 
GAAACGACAC CACCAACGCG TCGACGTGAC TTGGAAAGTT TCCGGCTTTA CGGTGACTCC 
ACAACAGCAG CGACGACAGC GGGCGCGGCA GCGGATGACA ACGCCAAAGC CGATGATTTA 
CGTCTATCCA CGCGTCAATT ACGTCGACAA CAACAACCAT TTGGTCAACT GTCCAGCAGC 
GCTTTAATCC AACGTTCCCA TTCAATTTCG ACGGACGATC TCAGTAACGA GTGGGAGTCG 
AACGAGGCGG TTGCTGAGTC CAACGAGTGG CGACGCGTCA GCAAATTGCG ACGCTCCTTT 
CAATCATCCT CTACGGCGAA CAATAGTAAT ACCAGCGCTC CCGCAGTGCA ACAACCGACG 
AGCTCTATAC GCCGTTCATA CGATCTGCCC GTCAGTGCTG TGAGCGTGTC ACGCATACGC 
GCCGAACTCG AAAGCGGTCG ACGTCTTACC ACAGCAATGC GTAATAATCA TGTGGATTTG 
GCTGCACTAG AGAGTATACT ACACGGTCCA GGCAAAACTA CGGACACCAG TAGCCGTAAT 
ACATTGCTTA CCGCCGAGTC ATTGAAAGAA ATACGTGGCC GTCTCAAGAA ATTATCGGAT 
GAATCATTGT ATAAGGACGA TTTTATAGCA TATCAACAAC CGCCGTCAGC AGACGAGCAC 
ATTGAAGTGC AGCAGAGCGT GCGTCGTAAA CTGTCCACAC CACGTTTGCA ACAATTGACA 
CCGGAAGAGT CGCCCGAGAT CTCAACGCCA ACACAGCATA CATCTGCCTT CAAAGTTGTG 
GGCGCACAAC GCAAGCCAAC AGACAACGAC AGCTTAACGC AGCGCTCAAC GCACTTGAAC 
CAAAAACACA CCTCAAACAG CTTGGAATCA CGTCAAAAGC AAAGAGATAC CAATTCAACC 
GAGTGGCACT CGCGTCGCAA ATCATATGGT TTCGAGAAGA TGTCACCGCC GGACAACAAA 
ACTATGCACC GCATGGACGC ATCCACCGAT AGCGGACTCG GACGTTCCGG CGAACTGGGC 
AATTGGTCAC CCACGGAAGC AGCACCACCG CGCGCCACAG TCATACATTT CGGCGAGCCC 
GCAAAAGCAG TCACTTCGGC GCACTACCGC GCCGCTAAAT CCCCAGTGCG CCGCACACCT 
GACGAGGACT TGCTCAAGCG ACACTCGATC GCTGTCGATG AGAGTCAATA TGTGCGCGAT 
AATTTGCGTA AAACCTCACA GGTGCATTTG AATGGTTTCT ATGATCCGGC CGAGCCCGCT 
GCCAAATCCA CAGTGCAGTT GAGCCGTTAT GACAGCAATG CGGAGAGTAA TCAACTCTTC 
CAACGCAACA CACAAAATGC ACAGAAACGT GTTGAGTTCT GCAAAACCGA GGTACATTTC 
GCCGCCGAAT CGGGGCGTGT CAATATCGTT GAAACCGATG GTAAACCGCC ACCGACAAAT 
AATTTCCGTC GACGCCGTCG CACCACAAGT GGACCGCTAC AGAGTCTTAC CAAAACCGCA 
AGCACGGCGG GTACAAGCAC AGCCGTGAGC ACAACAACCG CTAGTAATGT TACACATTTC 
GGTGATGATC AACATCAGCA GCAACGTCAT CGCACCATCG CCAGTTCGGT GGTCGCATAT 
AAAGCTACAT TAGTGGAACC ACCAACTGTG CTCAATGACG CACAAATAAT GCCGGCCACA 
ACTAACGTAA CGGTATCCGT ACAACATGCG GAGGGTTCAC GTGGCAGCTA CGCGTCAACA 
ACAAGCGGTG TCGATACGAC AGACAATGAA AATGACGAAA TCGCCGCTAT ACGCGGCATA 
CTCAAGAATA AACCGGTAAA GCCGAAACCT TATCATTTAG GTGAGAACAT CGATAGCGCC 
GATGCTTTGT GGGGTGTGCA GTTGAAGCCT GTGGCTGGTG GCGGCAGCAG CGCAGCGCGT 
GAGAAAGAAG ACTCACCCAC TGCTGTGTTG AATAAATCCG TTGCGGAACG CGTGCGCATT 
GTGGAGAAAC GTCATGAGCA TAACGCTCCA AATGGTTACT CTACGAAAAT CAACTTGAGT 
CTGGACGAAC CAGCTGCAGC AGCGCGTGAT TGGCCTAATG CAGGTGCGCC TATGCTAATG 
CCAAACACTG ACAATCAACC CATACCCAAC AACACAAATC GCCGTGCGAG CGCACAAGAG 
CTCATATTAC AAGATCTACG CGAACATCAG CGTATGCTCG ACGAGGGACT CAAGTCTACA 
TCGCTCATCA TCAAAACAAT GCGTTCGGCA AATGAGTTTG ATGAGGCTAT GCGGCGTCTA 
AGTATAGCTT CACTGGAATC GAGTACGATG CCACCAATTG TGGTGCCCAC ACCAATGTTG 
CGTTCGAATA GCTACCAGGA GACGGTACGT CGTTACTCCA ACGCCTCCTT GGAGAGCACC 
GAACGCGAAC TTACCACACA GACTGTAGTT CATACGAGTA GCAGTGCTGC AGCGTTAACA 
GCGGCCACAA GTAGTCGCAG ATTGTCGTTT GAAACACAAA GCGCTTTCGT AACACCGTTA 
AGCCTACCAC CAGCACCGCT AGTGGCACCA CGTCTAAAGC TGCTCGGCAG CACTGAGGTA 
TCAGTGAGTC AGCAGCTTTC ACAGTTGCGA CGCATGTACG ATATTGCAGC GACACAGCAA 
GAAAATGACG AAGATGCCGC CGACAGTGCC GATGAAGAGG TAAAAAGCTA CTTTTGCGCA 
CACGAACAAG CGGAACAAAG CGATAGTGGT GGCGGCACGT TGGAGAGCTC TTCGCAGGAA 
GATGAGCGCG CCATTGAATA CTCCAGCGGC AGTTGGAGTC GCATGAAGGC GAAGCGCACA 
ATTTGGAAGA TTGAAGCGGA AGAACGTAAA GCGGCGCCGG CCGTATTGGA CAAAGCAGAT 
CTACCTAAAT CGAACATTAT GAGCATAGAA CTACATTCAC CACAAACTTC GTTGGAGAAA 
CGTGATTCCA TCAAACGCAA CCAAACACCG CCCGTGGCCA AACCGAGAAC TGTGACTGGT 
GCAGTCAACG CCACTCAGCA TCCACAGATA CATGTGGCCA CCACAGTCAA GCAAACAGCT 
GATCGTTTTT CAGCAGCCGA ACAGCAACGC CAGTCACTGA AAATTGTCAA GGAAGCGCGT 
GGTGCACGTA AACTGCGTGA ACACGAACTC TCATACTTTG GCGTACCACA CGGACAGAAA 
CACGAAGAGA ACGAAACAAA ACCGAAGCAA CAGCCGAATA GCAACGCTAA TACGTTGAGT 
TTATCGAAGC GTTCGCTACC GTCCCGTCAA CGTTTAACTG TTATTGGTAA CGAGGATACG 
AAATCGATGC GTCGACATGA AGCAAACAAC ACTGAAACAA CAACCGTAAG CGGACAAACA 
CAAACGGCCA CTAAATGGCA ATTGTTGAAT GACAAACCCG ATCTACTACG ACACAGTCCG 
ATTGCTGTGG AACCACAATT GATACGACGT ACAGCAGATG TGCAGCGTAG TGAACGTAAT 
AGCACATCCG TGGAACGTAA CAAACGTATG CCCTCAAAAG AGTTGAGGTT AGATGATGAG 
CTCAATGATG CTGTTGAAGA TGATGATGAC GACAACGAGC ATTTTTATGA AAATATAACA 
AATGAAATTA CGCCTGTCTT CAAAGTGAAG TCACCGCAAC CGTATGATCG TAAAATGGAT 
ATGGAACGCG ACGCATTCAT ATTGAACGAG ATGAATGAGA ATGCCGATCT GACTATGAAG 
GCGCTCTCCG ATGAGGCCGC CCATAAAGAT CGGCGACGAC GACGTTCATC GCTCCATAGA 
CGCGACTCCA AACCACTAGA AACGATCGAA GAGAAATTGG TCACCCAAAA ACCCAACGAC 
ACATGCATTG CGGTGAAAAT GTCACGTGGC ACGCTAGCCT CCGAAGCCAA ACGCCACGCA 
CGTCACACAA CCACTTCACC CAGCCCACAC GATACAACCA CACGAGTGCG TACTTCATCA 
CAATCGTCGG TAGAAAGTTG TCCGCGTGCA CGTTCGCGTT CACTCTCCAG CGAACGTGAG 
TACGAAAACA TACGTACACC GAAACCGACG CGCACCAACG CAACAGCCAC AACGAAAATA 
CAAGCCAAAA GCGTTAGCAA ACAACAGCAG CGCTCCAGTT CACGTGAATC ACAGCGTTCA 
GCGCGTTTGC CATATTCATC GGGCGATGAG GTAAACCCAC GCGAGGAACA ACGTGTACGC 
ATGAAGAGTA AACGTAGCTC GAACTCTACG AGTGGCACAA CGGGTCGTAG TGGTGAACGA 
CAACGTAGCA GTAAGAAGGA TAGCCATCAC GACAAACATG GACATCACAC GACAACAACA 
AGCAGTCGAG ATGTTGAGCA GCGACGGCGC GCGGCCAGCA GCACTACGCA TGCCTCTCAG 
CGAGAGGAAC GCGGTGAGTG CGTGACTGTG CCGCAACAAC AGCGCACCGC AAGTAGTGGA 
CGCAGCAGTA GCAGCAACAA GCGACGTGAA GAACCTGCCA CCACCGCTGG CAGTAGCGGA 
CGTCACTCCA GCTCACGCAA TGCCACGGAG AAGGCACGCA CTAGCGCTGG TGTGCGTCTA 
TTGCGCTCGC TACGCGTCGG ACGTACGGAT GATGCCAAAG CGTCCAGCAC GGAGCAACGT 
GCGTCCGGAC ATCATCGTTC CACCAGCGAG CGCGAGAAGG CACATGAGAA AGAGCGCGAA 
AAGGAGTTGC AGTCCAAATC GCGAGGTCAC TCAACCAGCA AAACCCAGCA CCACAGCAGT 
AGTGAGCACC ACCAACATTC ACTAACCGCA CATAGCGGTA CAATGTCCAG TAAAACACGC 
GAACACAAGA AAGCAACCTC AAACGCCATT GAGAAGCCGC ACGAACGAGC CATCACACCA 
ACACACAGTA AAAGCTCGCA AAACATAAAT GGACGACATA CTACTACTGG ACAACATGAT 
GGCCACCACC GCAAACATAA CGATCATAAA CGAGCACACA CACGACCACA ACAAGAAGAG 
AGCAGAACAA CGAAGACAAC GACGACGCAG CGAAAGCGTA GTGAACATAG CGCAAGGGAG 
CGCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028893773.1
CDS	948..6776
Translation	MEVATTNTHHHNHHHQHLGHQLHHPAHRLPMAKSTQHQQQQQQSNHCLTVSNDSAQSGGGGGGGSPRSPFQREVREWQRIYPDTGALLSGRLEADRWINGPLNSYGKKSSSGGSSSSNNSTKLNTHKSAGGKVSLHAIVGSAGDNNTNSGSASRINNDLTESPKSTTTTAKSSNEALSGTKTWQQQQRRSNARHLEQATLYGQNSNSSKMRVGGADCDDCYAVVSADADESQLRYHQHHLQPQQLRKQQQQQQQSSVVLSETTPPTRRRDLESFRLYGDSTTAATTAGAAADDNAKADDLRLSTRQLRRQQQPFGQLSSSALIQRSHSISTDDLSNEWESNEAVAESNEWRRVSKLRRSFQSSSTANNSNTSAPAVQQPTSSIRRSYDLPVSAVSVSRIRAELESGRRLTTAMRNNHVDLAALESILHGPGKTTDTSSRNTLLTAESLKEIRGRLKKLSDESLYKDDFIAYQQPPSADEHIEVQQSVRRKLSTPRLQQLTPEESPEISTPTQHTSAFKVVGAQRKPTDNDSLTQRSTHLNQKHTSNSLESRQKQRDTNSTEWHSRRKSYGFEKMSPPDNKTMHRMDASTDSGLGRSGELGNWSPTEAAPPRATVIHFGEPAKAVTSAHYRAAKSPVRRTPDEDLLKRHSIAVDESQYVRDNLRKTSQVHLNGFYDPAEPAAKSTVQLSRYDSNAESNQLFQRNTQNAQKRVEFCKTEVHFAAESGRVNIVETDGKPPPTNNFRRRRRTTSGPLQSLTKTASTAGTSTAVSTTTASNVTHFGDDQHQQQRHRTIASSVVAYKATLVEPPTVLNDAQIMPATTNVTVSVQHAEGSRGSYASTTSGVDTTDNENDEIAAIRGILKNKPVKPKPYHLGENIDSADALWGVQLKPVAGGGSSAAREKEDSPTAVLNKSVAERVRIVEKRHEHNAPNGYSTKINLSLDEPAAAARDWPNAGAPMLMPNTDNQPIPNNTNRRASAQELILQDLREHQRMLDEGLKSTSLIIKTMRSANEFDEAMRRLSIASLESSTMPPIVVPTPMLRSNSYQETVRRYSNASLESTERELTTQTVVHTSSSAAALTAATSSRRLSFETQSAFVTPLSLPPAPLVAPRLKLLGSTEVSVSQQLSQLRRMYDIAATQQENDEDAADSADEEVKSYFCAHEQAEQSDSGGGTLESSSQEDERAIEYSSGSWSRMKAKRTIWKIEAEERKAAPAVLDKADLPKSNIMSIELHSPQTSLEKRDSIKRNQTPPVAKPRTVTGAVNATQHPQIHVATTVKQTADRFSAAEQQRQSLKIVKEARGARKLREHELSYFGVPHGQKHEENETKPKQQPNSNANTLSLSKRSLPSRQRLTVIGNEDTKSMRRHEANNTETTTVSGQTQTATKWQLLNDKPDLLRHSPIAVEPQLIRRTADVQRSERNSTSVERNKRMPSKELRLDDELNDAVEDDDDDNEHFYENITNEITPVFKVKSPQPYDRKMDMERDAFILNEMNENADLTMKALSDEAAHKDRRRRRSSLHRRDSKPLETIEEKLVTQKPNDTCIAVKMSRGTLASEAKRHARHTTTSPSPHDTTTRVRTSSQSSVESCPRARSRSLSSEREYENIRTPKPTRTNATATTKIQAKSVSKQQQRSSSRESQRSARLPYSSGDEVNPREEQRVRMKSKRSSNSTSGTTGRSGERQRSSKKDSHHDKHGHHTTTTSSRDVEQRRRAASSTTHASQREERGECVTVPQQQRTASSGRSSSSNKRREEPATTAGSSGRHSSSRNATEKARTSAGVRLLRSLRVGRTDDAKASSTEQRASGHHRSTSEREKAHEKEREKELQSKSRGHSTSKTQHHSSSEHHQHSLTAHSGTMSSKTREHKKATSNAIEKPHERAITPTHSKSSQNINGRHTTTGQHDGHHRKHNDHKRAHTRPQQEESRTTKTTTTQRKRSEHSARERK

Target ORF information:

RefSeq Version	XM_029037940.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029037940.1

ORF Insert Sequence:

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GAAAAGCAGC AGCGGCGGCA GCAGCAGCAG CAACAACTCC 
ACAAAGCTGA ACACCCACAA GAGTGCTGGC GGTAAGGTGA GCCTACATGC AATTGTTGGC 
AGCGCTGGTG ACAACAACAC CAACAGCGGC AGCGCTAGTC GAATCAACAA CGACTTAACA 
GAGTCACCAA AAAGCACAAC AACAACAGCG AAAAGTTCGA ATGAAGCATT GAGTGGTACA 
AAAACGTGGC AGCAGCAGCA GCGGCGTAGC AACGCTCGTC ATTTGGAACA AGCTACTCTA 
TATGGCCAAA ACAGCAATTC ATCTAAAATG CGCGTAGGCG GCGCTGATTG TGATGATTGT 
TATGCGGTTG TGTCGGCTGA CGCAGATGAG TCGCAGCTGC GCTACCACCA ACACCACCTT 
CAACCGCAAC AGCTACGTAA ACAACAACAA CAGCAGCAAC AAAGCAGTGT TGTGCTAAGT 
GAAACGACAC CACCAACGCG TCGACGTGAC TTGGAAAGTT TCCGGCTTTA CGGTGACTCC 
ACAACAGCAG CGACGACAGC GGGCGCGGCA GCGGATGACA ACGCCAAAGC CGATGATTTA 
CGTCTATCCA CGCGTCAATT ACGTCGACAA CAACAACCAT TTGGTCAACT GTCCAGCAGC 
GCTTTAATCC AACGTTCCCA TTCAATTTCG ACGGACGATC TCAGTAACGA GTGGGAGTCG 
AACGAGGCGG TTGCTGAGTC CAACGAGTGG CGACGCGTCA GCAAATTGCG ACGCTCCTTT 
CAATCATCCT CTACGGCGAA CAATAGTAAT ACCAGCGCTC CCGCAGTGCA ACAACCGACG 
AGCTCTATAC GCCGTTCATA CGATCTGCCC GTCAGTGCTG TGAGCGTGTC ACGCATACGC 
GCCGAACTCG AAAGCGGTCG ACGTCTTACC ACAGCAATGC GTAATAATCA TGTGGATTTG 
GCTGCACTAG AGAGTATACT ACACGGTCCA GGCAAAACTA CGGACACCAG TAGCCGTAAT 
ACATTGCTTA CCGCCGAGTC ATTGAAAGAA ATACGTGGCC GTCTCAAGAA ATTATCGGAT 
GAATCATTGT ATAAGGACGA TTTTATAGCA TATCAACAAC CGCCGTCAGC AGACGAGCAC 
ATTGAAGTGC AGCAGAGCGT GCGTCGTAAA CTGTCCACAC CACGTTTGCA ACAATTGACA 
CCGGAAGAGT CGCCCGAGAT CTCAACGCCA ACACAGCATA CATCTGCCTT CAAAGTTGTG 
GGCGCACAAC GCAAGCCAAC AGACAACGAC AGCTTAACGC AGCGCTCAAC GCACTTGAAC 
CAAAAACACA CCTCAAACAG CTTGGAATCA CGTCAAAAGC AAAGAGATAC CAATTCAACC 
GAGTGGCACT CGCGTCGCAA ATCATATGGT TTCGAGAAGA TGTCACCGCC GGACAACAAA 
ACTATGCACC GCATGGACGC ATCCACCGAT AGCGGACTCG GACGTTCCGG CGAACTGGGC 
AATTGGTCAC CCACGGAAGC AGCACCACCG CGCGCCACAG TCATACATTT CGGCGAGCCC 
GCAAAAGCAG TCACTTCGGC GCACTACCGC GCCGCTAAAT CCCCAGTGCG CCGCACACCT 
GACGAGGACT TGCTCAAGCG ACACTCGATC GCTGTCGATG AGAGTCAATA TGTGCGCGAT 
AATTTGCGTA AAACCTCACA GGTGCATTTG AATGGTTTCT ATGATCCGGC CGAGCCCGCT 
GCCAAATCCA CAGTGCAGTT GAGCCGTTAT GACAGCAATG CGGAGAGTAA TCAACTCTTC 
CAACGCAACA CACAAAATGC ACAGAAACGT GTTGAGTTCT GCAAAACCGA GGTACATTTC 
GCCGCCGAAT CGGGGCGTGT CAATATCGTT GAAACCGATG GTAAACCGCC ACCGACAAAT 
AATTTCCGTC GACGCCGTCG CACCACAAGT GGACCGCTAC AGAGTCTTAC CAAAACCGCA 
AGCACGGCGG GTACAAGCAC AGCCGTGAGC ACAACAACCG CTAGTAATGT TACACATTTC 
GGTGATGATC AACATCAGCA GCAACGTCAT CGCACCATCG CCAGTTCGGT GGTCGCATAT 
AAAGCTACAT TAGTGGAACC ACCAACTGTG CTCAATGACG CACAAATAAT GCCGGCCACA 
ACTAACGTAA CGGTATCCGT ACAACATGCG GAGGGTTCAC GTGGCAGCTA CGCGTCAACA 
ACAAGCGGTG TCGATACGAC AGACAATGAA AATGACGAAA TCGCCGCTAT ACGCGGCATA 
CTCAAGAATA AACCGGTAAA GCCGAAACCT TATCATTTAG GTGAGAACAT CGATAGCGCC 
GATGCTTTGT GGGGTGTGCA GTTGAAGCCT GTGGCTGGTG GCGGCAGCAG CGCAGCGCGT 
GAGAAAGAAG ACTCACCCAC TGCTGTGTTG AATAAATCCG TTGCGGAACG CGTGCGCATT 
GTGGAGAAAC GTCATGAGCA TAACGCTCCA AATGGTTACT CTACGAAAAT CAACTTGAGT 
CTGGACGAAC CAGCTGCAGC AGCGCGTGAT TGGCCTAATG CAGGTGCGCC TATGCTAATG 
CCAAACACTG ACAATCAACC CATACCCAAC AACACAAATC GCCGTGCGAG CGCACAAGAG 
CTCATATTAC AAGATCTACG CGAACATCAG CGTATGCTCG ACGAGGGACT CAAGTCTACA 
TCGCTCATCA TCAAAACAAT GCGTTCGGCA AATGAGTTTG ATGAGGCTAT GCGGCGTCTA 
AGTATAGCTT CACTGGAATC GAGTACGATG CCACCAATTG TGGTGCCCAC ACCAATGTTG 
CGTTCGAATA GCTACCAGGA GACGGTACGT CGTTACTCCA ACGCCTCCTT GGAGAGCACC 
GAACGCGAAC TTACCACACA GACTGTAGTT CATACGAGTA GCAGTGCTGC AGCGTTAACA 
GCGGCCACAA GTAGTCGCAG ATTGTCGTTT GAAACACAAA GCGCTTTCGT AACACCGTTA 
AGCCTACCAC CAGCACCGCT AGTGGCACCA CGTCTAAAGC TGCTCGGCAG CACTGAGGTA 
TCAGTGAGTC AGCAGCTTTC ACAGTTGCGA CGCATGTACG ATATTGCAGC GACACAGCAA 
GAAAATGACG AAGATGCCGC CGACAGTGCC GATGAAGAGG TAAAAAGCTA CTTTTGCGCA 
CACGAACAAG CGGAACAAAG CGATAGTGGT GGCGGCACGT TGGAGAGCTC TTCGCAGGAA 
GATGAGCGCG CCATTGAATA CTCCAGCGGC AGTTGGAGTC GCATGAAGGC GAAGCGCACA 
ATTTGGAAGA TTGAAGCGGA AGAACGTAAA GCGGCGCCGG CCGTATTGGA CAAAGCAGAT 
CTACCTAAAT CGAACATTAT GAGCATAGAA CTACATTCAC CACAAACTTC GTTGGAGAAA 
CGTGATTCCA TCAAACGCAA CCAAACACCG CCCGTGGCCA AACCGAGAAC TGTGACTGGT 
GCAGTCAACG CCACTCAGCA TCCACAGATA CATGTGGCCA CCACAGTCAA GCAAACAGCT 
GATCGTTTTT CAGCAGCCGA ACAGCAACGC CAGTCACTGA AAATTGTCAA GGAAGCGCGT 
GGTGCACGTA AACTGCGTGA ACACGAACTC TCATACTTTG GCGTACCACA CGGACAGAAA 
CACGAAGAGA ACGAAACAAA ACCGAAGCAA CAGCCGAATA GCAACGCTAA TACGTTGAGT 
TTATCGAAGC GTTCGCTACC GTCCCGTCAA CGTTTAACTG TTATTGGTAA CGAGGATACG 
AAATCGATGC GTCGACATGA AGCAAACAAC ACTGAAACAA CAACCGTAAG CGGACAAACA 
CAAACGGCCA CTAAATGGCA ATTGTTGAAT GACAAACCCG ATCTACTACG ACACAGTCCG 
ATTGCTGTGG AACCACAATT GATACGACGT ACAGCAGATG TGCAGCGTAG TGAACGTAAT 
AGCACATCCG TGGAACGTAA CAAACGTATG CCCTCAAAAG AGTTGAGGTT AGATGATGAG 
CTCAATGATG CTGTTGAAGA TGATGATGAC GACAACGAGC ATTTTTATGA AAATATAACA 
AATGAAATTA CGCCTGTCTT CAAAGTGAAG TCACCGCAAC CGTATGATCG TAAAATGGAT 
ATGGAACGCG ACGCATTCAT ATTGAACGAG ATGAATGAGA ATGCCGATCT GACTATGAAG 
GCGCTCTCCG ATGAGGCCGC CCATAAAGAT CGGCGACGAC GACGTTCATC GCTCCATAGA 
CGCGACTCCA AACCACTAGA AACGATCGAA GAGAAATTGG TCACCCAAAA ACCCAACGAC 
ACATGCATTG CGGTGAAAAT GTCACGTGGC ACGCTAGCCT CCGAAGCCAA ACGCCACGCA 
CGTCACACAA CCACTTCACC CAGCCCACAC GATACAACCA CACGAGTGCG TACTTCATCA 
CAATCGTCGG TAGAAAGTTG TCCGCGTGCA CGTTCGCGTT CACTCTCCAG CGAACGTGAG 
TACGAAAACA TACGTACACC GAAACCGACG CGCACCAACG CAACAGCCAC AACGAAAATA 
CAAGCCAAAA GCGTTAGCAA ACAACAGCAG CGCTCCAGTT CACGTGAATC ACAGCGTTCA 
GCGCGTTTGC CATATTCATC GGGCGATGAG GTAAACCCAC GCGAGGAACA ACGTGTACGC 
ATGAAGAGTA AACGTAGCTC GAACTCTACG AGTGGCACAA CGGGTCGTAG TGGTGAACGA 
CAACGTAGCA GTAAGAAGGA TAGCCATCAC GACAAACATG GACATCACAC GACAACAACA 
AGCAGTCGAG ATGTTGAGCA GCGACGGCGC GCGGCCAGCA GCACTACGCA TGCCTCTCAG 
CGAGAGGAAC GCGGTGAGTG CGTGACTGTG CCGCAACAAC AGCGCACCGC AAGTAGTGGA 
CGCAGCAGTA GCAGCAACAA GCGACGTGAA GAACCTGCCA CCACCGCTGG CAGTAGCGGA 
CGTCACTCCA GCTCACGCAA TGCCACGGAG AAGGCACGCA CTAGCGCTGG TGTGCGTCTA 
TTGCGCTCGC TACGCGTCGG ACGTACGGAT GATGCCAAAG CGTCCAGCAC GGAGCAACGT 
GCGTCCGGAC ATCATCGTTC CACCAGCGAG CGCGAGAAGG CACATGAGAA AGAGCGCGAA 
AAGGAGTTGC AGTCCAAATC GCGAGGTCAC TCAACCAGCA AAACCCAGCA CCACAGCAGT 
AGTGAGCACC ACCAACATTC ACTAACCGCA CATAGCGGTA CAATGTCCAG TAAAACACGC 
GAACACAAGA AAGCAACCTC AAACGCCATT GAGAAGCCGC ACGAACGAGC CATCACACCA 
ACACACAGTA AAAGCTCGCA AAACATAAAT GGACGACATA CTACTACTGG ACAACATGAT 
GGCCACCACC GCAAACATAA CGATCATAAA CGAGCACACA CACGACCACA ACAAGAAGAG 
AGCAGAACAA CGAAGACAAC GACGACGCAG CGAAAGCGTA GTGAACATAG CGCAAGGGAG 
CGCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208490 ( XM_029037941.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028893774.1 Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X9, mRNA.

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CloneID	OBa254490
Clone ID Related Accession (Same CDS sequence)	XM_029037941.1
Accession Version	XM_029037941.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105208490 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863673.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGA TCTACCTAAA TCGAACATTA TGAGCATAGA ACTACATTCA 
CCACAAACTT CGTTGGAGAA ACGTGATTCC ATCAAACGCA ACCAAACACC GCCCGTGGCC 
AAACCGAGAA CTGTGACTGG TGCAGTCAAC GCCACTCAGC ATCCACAGAT ACATGTGGCC 
ACCACAGTCA AGCAAACAGC TGATCGTTTT TCAGCAGCCG AACAGCAACG CCAGTCACTG 
AAAATTGTCA AGGAAGCGCG TGGTGCACGT AAACTGCGTG AACACGAACT CTCATACTTT 
GGCGTACCAC ACGGACAGAA ACACGAAGAG AACGAAACAA AACCGAAGCA ACAGCCGAAT 
AGCAACGCTA ATACGTTGAG TTTATCGAAG CGTTCGCTAC CGTCCCGTCA ACGTTTAACT 
GTTATTGGTA ACGAGGATAC GAAATCGATG CGTCGACATG AAGCAAACAA CACTGAAACA 
ACAACCGTAA GCGGACAAAC ACAAACGGCC ACTAAATGGC AATTGTTGAA TGACAAACCC 
GATCTACTAC GACACAGTCC GATTGCTGTG GAACCACAAT TGATACGACG TACAGCAGAT 
GTGCAGCGTA GTGAACGTAA TAGCACATCC GTGGAACGTA ACAAACGTAT GCCCTCAAAA 
GAGTTGAGGT TAGATGATGA GCTCAATGAT GCTGTTGAAG ATGATGATGA CGACAACGAG 
CATTTTTATG AAAATATAAC AAATGAAATT ACGCCTGTCT TCAAAGTGAA GTCACCGCAA 
CCGTATGATC GTAAAATGGA TATGGAACGC GACGCATTCA TATTGAACGA GATGAATGAG 
AATGCCGATC TGACTATGAA GGCGCTCTCC GATGAGGCCG CCCATAAAGA TCGGCGACGA 
CGACGTTCAT CGCTCCATAG ACGCGACTCC AAACCACTAG AAACGATCGA AGAGAAATTG 
GTCACCCAAA AACCCAACGA CACATGCATT GCGGTGAAAA TGTCACGTGG CACGCTAGCC 
TCCGAAGCCA AACGCCACGC ACGTCACACA ACCACTTCAC CCAGCCCACA CGATACAACC 
ACACGAGTGC GTACTTCATC ACAATCGTCG GTAGAAAGTT GTCCGCGTGC ACGTTCGCGT 
TCACTCTCCA GCGAACGTGA GTACGAAAAC ATACGTACAC CGAAACCGAC GCGCACCAAC 
GCAACAGCCA CAACGAAAAT ACAAGCCAAA AGCGTTAGCA AACAACAGCA GCGCTCCAGT 
TCACGTGAAT CACAGCGTTC AGCGCGTTTG CCATATTCAT CGGGCGATGA GGTAAACCCA 
CGCGAGGAAC AACGTGTACG CATGAAGAGT AAACGTAGCT CGAACTCTAC GAGTGGCACA 
ACGGGTCGTA GTGGTGAACG ACAACGTAGC AGTAAGAAGG ATAGCCATCA CGACAAACAT 
GGACATCACA CGACAACAAC AAGCAGTCGA GATGTTGAGC AGCGACGGCG CGCGGCCAGC 
AGCACTACGC ATGCCTCTCA GCGAGAGGAA CGCGGTGAGT GCGTGACTGT GCCGCAACAA 
CAGCGCACCG CAAGTAGTGG ACGCAGCAGT AGCAGCAACA AGCGACGTGA AGAACCTGCC 
ACCACCGCTG GCAGTAGCGG ACGTCACTCC AGCTCACGCA ATGCCACGGA GAAGGCACGC 
ACTAGCGCTG GTGTGCGTCT ATTGCGCTCG CTACGCGTCG GACGTACGGA TGATGCCAAA 
GCGTCCAGCA CGGAGCAACG TGCGTCCGGA CATCATCGTT CCACCAGCGA GCGCGAGAAG 
GCACATGAGA AAGAGCGCGA AAAGGAGTTG CAGTCCAAAT CGCGAGGTCA CTCAACCAGC 
AAAACCCAGC ACCACAGCAG TAGTGAGCAC CACCAACATT CACTAACCGC ACATAGCGGT 
ACAATGTCCA GTAAAACACG CGAACACAAG AAAGCAACCT CAAACGCCAT TGAGAAGCCG 
CACGAACGAG CCATCACACC AACACACAGT AAAAGCTCGC AAAACATAAA TGGACGACAT 
ACTACTACTG GACAACATGA TGGCCACCAC CGCAAACATA ACGATCATAA ACGAGCACAC 
ACACGACCAC AACAAGAAGA GAGCAGAACA ACGAAGACAA CGACGACGCA GCGAAAGCGT 
AGTGAACATA GCGCAAGGGA GCGCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028893774.1
CDS	948..6677
Translation	MEVATTNTHHHNHHHQHLGHQLHHPAHRLPMAKSTQHQQQQQQSNHCLTVSNDSAQSGGGGGGGSPRSPFQREVREWQRIYPDTGALLSGRLEADRWINGPLNSYGKISDSQNISQPNGTQHTQRKQLEVLQKRTANGAVQVIRAQTVQTSSSRSWSSSNSNSASTSASASASASPICMQANVVGARSLLHNHTNNNINNINSSKHFHSTLQQHLAEANTHWLQLSPPTIAATVDISEVSTDDDLDKAVPTIAETPYIVDDSEIDVGNGCGVDADINASATLCNYSISRSGSAGSGGGGSSKGHALLSSAASSSSTLHSQSSALLVPDTAPSLKLSNLMKSSSGGSSSSNNSTKLNTHKSAGGKVSLHAIVGSAGDNNTNSGSASRINNDLTESPKSTTTTAKSSNEALSGTKTWQQQQRRSNARHLEQATLYGQNSNSSKMRVGGADCDDCYAVVSADADESQLRYHQHHLQPQQLRKQQQQQQQSSVVLSETTPPTRRRDLESFRLYGDSTTAATTAGAAADDNAKADDLRLSTRQLRRQQQPFGQLSSSALIQRSHSISTDDLSNEWESNEAVAESNEWRRVSKLRRSFQSSSTANNSNTSAPAVQQPTSSIRRSYDLPVSAVSVSRIRAELESGRRLTTAMRNNHVDLAALESILHGPGKTTDTSSRNTLLTAESLKEIRGRLKKLSDESLYKDDFIAYQQPPSADEHIEVQQSVRRKLSTPRLQQLTPEESPEISTPTQHTSAFKVVGAQRKPTDNDSLTQRSTHLNQKHTSNSLESRQKQRDTNSTEWHSRRKSYGFEKMSPPDNKTMHRMDASTDSGLGRSGELGNWSPTEAAPPRATVIHFGEPAKAVTSAHYRAAKSPVRRTPDEDLLKRHSIAVDESQYVRDNLRKTSQVHLNGFYDPAEPAAKSTVQLSRYDSNAESNQLFQRNTQNAQKRVEFCKTEVHFAAESGRVNIVETDGKPPPTNNFRRRRRTTSGPLQSLTKTASTAGTSTAVSTTTASNVTHFGDDQHQQQRHRTIASSVVAYKATLVEPPTVLNDAQIMPATTNVTVSVQHAEGSRGSYASTTSGVDTTDNENDEIAAIRGILKNKPVKPKPYHLGENIDSADALWGVQLKPVAGGGSSAAREKEDSPTAVLNKSVAERVRIVEKRHEHNAPNGYSTKINLSLDEPAAAARDWPNADLPKSNIMSIELHSPQTSLEKRDSIKRNQTPPVAKPRTVTGAVNATQHPQIHVATTVKQTADRFSAAEQQRQSLKIVKEARGARKLREHELSYFGVPHGQKHEENETKPKQQPNSNANTLSLSKRSLPSRQRLTVIGNEDTKSMRRHEANNTETTTVSGQTQTATKWQLLNDKPDLLRHSPIAVEPQLIRRTADVQRSERNSTSVERNKRMPSKELRLDDELNDAVEDDDDDNEHFYENITNEITPVFKVKSPQPYDRKMDMERDAFILNEMNENADLTMKALSDEAAHKDRRRRRSSLHRRDSKPLETIEEKLVTQKPNDTCIAVKMSRGTLASEAKRHARHTTTSPSPHDTTTRVRTSSQSSVESCPRARSRSLSSEREYENIRTPKPTRTNATATTKIQAKSVSKQQQRSSSRESQRSARLPYSSGDEVNPREEQRVRMKSKRSSNSTSGTTGRSGERQRSSKKDSHHDKHGHHTTTTSSRDVEQRRRAASSTTHASQREERGECVTVPQQQRTASSGRSSSSNKRREEPATTAGSSGRHSSSRNATEKARTSAGVRLLRSLRVGRTDDAKASSTEQRASGHHRSTSEREKAHEKEREKELQSKSRGHSTSKTQHHSSSEHHQHSLTAHSGTMSSKTREHKKATSNAIEKPHERAITPTHSKSSQNINGRHTTTGQHDGHHRKHNDHKRAHTRPQQEESRTTKTTTTQRKRSEHSARERK

Target ORF information:

RefSeq Version	XM_029037941.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029037941.1

ORF Insert Sequence:

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGA TCTACCTAAA TCGAACATTA TGAGCATAGA ACTACATTCA 
CCACAAACTT CGTTGGAGAA ACGTGATTCC ATCAAACGCA ACCAAACACC GCCCGTGGCC 
AAACCGAGAA CTGTGACTGG TGCAGTCAAC GCCACTCAGC ATCCACAGAT ACATGTGGCC 
ACCACAGTCA AGCAAACAGC TGATCGTTTT TCAGCAGCCG AACAGCAACG CCAGTCACTG 
AAAATTGTCA AGGAAGCGCG TGGTGCACGT AAACTGCGTG AACACGAACT CTCATACTTT 
GGCGTACCAC ACGGACAGAA ACACGAAGAG AACGAAACAA AACCGAAGCA ACAGCCGAAT 
AGCAACGCTA ATACGTTGAG TTTATCGAAG CGTTCGCTAC CGTCCCGTCA ACGTTTAACT 
GTTATTGGTA ACGAGGATAC GAAATCGATG CGTCGACATG AAGCAAACAA CACTGAAACA 
ACAACCGTAA GCGGACAAAC ACAAACGGCC ACTAAATGGC AATTGTTGAA TGACAAACCC 
GATCTACTAC GACACAGTCC GATTGCTGTG GAACCACAAT TGATACGACG TACAGCAGAT 
GTGCAGCGTA GTGAACGTAA TAGCACATCC GTGGAACGTA ACAAACGTAT GCCCTCAAAA 
GAGTTGAGGT TAGATGATGA GCTCAATGAT GCTGTTGAAG ATGATGATGA CGACAACGAG 
CATTTTTATG AAAATATAAC AAATGAAATT ACGCCTGTCT TCAAAGTGAA GTCACCGCAA 
CCGTATGATC GTAAAATGGA TATGGAACGC GACGCATTCA TATTGAACGA GATGAATGAG 
AATGCCGATC TGACTATGAA GGCGCTCTCC GATGAGGCCG CCCATAAAGA TCGGCGACGA 
CGACGTTCAT CGCTCCATAG ACGCGACTCC AAACCACTAG AAACGATCGA AGAGAAATTG 
GTCACCCAAA AACCCAACGA CACATGCATT GCGGTGAAAA TGTCACGTGG CACGCTAGCC 
TCCGAAGCCA AACGCCACGC ACGTCACACA ACCACTTCAC CCAGCCCACA CGATACAACC 
ACACGAGTGC GTACTTCATC ACAATCGTCG GTAGAAAGTT GTCCGCGTGC ACGTTCGCGT 
TCACTCTCCA GCGAACGTGA GTACGAAAAC ATACGTACAC CGAAACCGAC GCGCACCAAC 
GCAACAGCCA CAACGAAAAT ACAAGCCAAA AGCGTTAGCA AACAACAGCA GCGCTCCAGT 
TCACGTGAAT CACAGCGTTC AGCGCGTTTG CCATATTCAT CGGGCGATGA GGTAAACCCA 
CGCGAGGAAC AACGTGTACG CATGAAGAGT AAACGTAGCT CGAACTCTAC GAGTGGCACA 
ACGGGTCGTA GTGGTGAACG ACAACGTAGC AGTAAGAAGG ATAGCCATCA CGACAAACAT 
GGACATCACA CGACAACAAC AAGCAGTCGA GATGTTGAGC AGCGACGGCG CGCGGCCAGC 
AGCACTACGC ATGCCTCTCA GCGAGAGGAA CGCGGTGAGT GCGTGACTGT GCCGCAACAA 
CAGCGCACCG CAAGTAGTGG ACGCAGCAGT AGCAGCAACA AGCGACGTGA AGAACCTGCC 
ACCACCGCTG GCAGTAGCGG ACGTCACTCC AGCTCACGCA ATGCCACGGA GAAGGCACGC 
ACTAGCGCTG GTGTGCGTCT ATTGCGCTCG CTACGCGTCG GACGTACGGA TGATGCCAAA 
GCGTCCAGCA CGGAGCAACG TGCGTCCGGA CATCATCGTT CCACCAGCGA GCGCGAGAAG 
GCACATGAGA AAGAGCGCGA AAAGGAGTTG CAGTCCAAAT CGCGAGGTCA CTCAACCAGC 
AAAACCCAGC ACCACAGCAG TAGTGAGCAC CACCAACATT CACTAACCGC ACATAGCGGT 
ACAATGTCCA GTAAAACACG CGAACACAAG AAAGCAACCT CAAACGCCAT TGAGAAGCCG 
CACGAACGAG CCATCACACC AACACACAGT AAAAGCTCGC AAAACATAAA TGGACGACAT 
ACTACTACTG GACAACATGA TGGCCACCAC CGCAAACATA ACGATCATAA ACGAGCACAC 
ACACGACCAC AACAAGAAGA GAGCAGAACA ACGAAGACAA CGACGACGCA GCGAAAGCGT 
AGTGAACATA GCGCAAGGGA GCGCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208490 ( XM_011178364.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176666.1 Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X5, mRNA.

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CloneID	OBa194182
Clone ID Related Accession (Same CDS sequence)	XM_011178364.1
Accession Version	XM_011178364.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105208490 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863673.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GAAAAGCAGC AGCGGCGGCA GCAGCAGCAG CAACAACTCC 
ACAAAGCTGA ACACCCACAA GAGTGCTGGC GGTAAGGTGA GCCTACATGC AATTGTTGGC 
AGCGCTGGTG ACAACAACAC CAACAGCGGC AGCGCTAGTC GAATCAACAA CGACTTAACA 
GAGTCACCAA AAAGCACAAC AACAACAGCG AAAAGTTCGA ATGAAGCATT GAGTGGTACA 
AAAACGTGGC AGCAGCAGCA GCGGCGTAGC AACGCTCGTC ATTTGGAACA AGCTACTCTA 
TATGGCCAAA ACAGCAATTC ATCTAAAATG CGCGTAGGCG GCGCTGATTG TGATGATTGT 
TATGCGGTTG TGTCGGCTGA CGCAGATGAG TCGCAGCTGC GCTACCACCA ACACCACCTT 
CAACCGCAAC AGCTACGTAA ACAACAACAA CAGCAGCAAC AAAGCAGTGT TGTGCTAAGT 
GAAACGACAC CACCAACGCG TCGACGTGAC TTGGAAAGTT TCCGGCTTTA CGGTGACTCC 
ACAACAGCAG CGACGACAGC GGGCGCGGCA GCGGATGACA ACGCCAAAGC CGATGATTTA 
CGTCTATCCA CGCGTCAATT ACGTCGACAA CAACAACCAT TTGGTCAACT GTCCAGCAGC 
GCTTTAATCC AACGTTCCCA TTCAATTTCG ACGGACGATC TCAGTAACGA GTGGGAGTCG 
AACGAGGCGG TTGCTGAGTC CAACGAGTGG CGACGCGTCA GCAAATTGCG ACGCTCCTTT 
CAATCATCCT CTACGGCGAA CAATAGTAAT ACCAGCGCTC CCGCAGTGCA ACAACCGACG 
AGCTCTATAC GCCGTTCATA CGATCTGCCC GTCAGTGCTG TGAGCGTGTC ACGCATACGC 
GCCGAACTCG AAAGCGGTCG ACGTCTTACC ACAGCAATGC GTAATAATCA TGTGGATTTG 
GCTGCACTAG AGAGTATACT ACACGGTCCA GGCAAAACTA CGGACACCAG TAGCCGTAAT 
ACATTGCTTA CCGCCGAGTC ATTGAAAGAA ATACGTGGCC GTCTCAAGAA ATTATCGGAT 
GAATCATTGT ATAAGGACGA TTTTATAGCA TATCAACAAC CGCCGTCAGC AGACGAGCAC 
ATTGAAGTGC AGCAGAGCGT GCGTCGTAAA CTGTCCACAC CACGTTTGCA ACAATTGACA 
CCGGAAGAGT CGCCCGAGAT CTCAACGCCA ACACAGCATA CATCTGCCTT CAAAGTTGTG 
GGCGCACAAC GCAAGCCAAC AGACAACGAC AGCTTAACGC AGCGCTCAAC GCACTTGAAC 
CAAAAACACA CCTCAAACAG CTTGGAATCA CGTCAAAAGC AAAGAGATAC CAATTCAACC 
GAGTGGCACT CGCGTCGCAA ATCATATGGT TTCGAGAAGA TGTCACCGCC GGACAACAAA 
ACTATGCACC GCATGGACGC ATCCACCGAT AGCGGACTCG GACGTTCCGG CGAACTGGGC 
AATTGGTCAC CCACGGAAGC AGCACCACCG CGCGCCACAG TCATACATTT CGGCGAGCCC 
GCAAAAGCAG TCACTTCGGC GCACTACCGC GCCGCTAAAT CCCCAGTGCG CCGCACACCT 
GACGAGGACT TGCTCAAGCG ACACTCGATC GCTGTCGATG AGAGTCAATA TGTGCGCGAT 
AATTTGCGTA AAACCTCACA GGTGCATTTG AATGGTTTCT ATGATCCGGC CGAGCCCGCT 
GCCAAATCCA CAGTGCAGTT GAGCCGTTAT GACAGCAATG CGGAGAGTAA TCAACTCTTC 
CAACGCAACA CACAAAATGC ACAGAAACGT GTTGAGTTCT GCAAAACCGA GGTACATTTC 
GCCGCCGAAT CGGGGCGTGT CAATATCGTT GAAACCGATG GTAAACCGCC ACCGACAAAT 
AATTTCCGTC GACGCCGTCG CACCACAAGT GGACCGCTAC AGAGTCTTAC CAAAACCGCA 
AGCACGGCGG GTACAAGCAC AGCCGTGAGC ACAACAACCG CTAGTAATGT TACACATTTC 
GGTGATGATC AACATCAGCA GCAACGTCAT CGCACCATCG CCAGTTCGGT GGTCGCATAT 
AAAGCTACAT TAGTGGAACC ACCAACTGTG CTCAATGACG CACAAATAAT GCCGGCCACA 
ACTAACGTAA CGGTATCCGT ACAACATGCG GAGGGTTCAC GTGGCAGCTA CGCGTCAACA 
ACAAGCGGTG TCGATACGAC AGACAATGAA AATGACGAAA TCGCCGCTAT ACGCGGCATA 
CTCAAGAATA AACCGGTAAA GCCGAAACCT TATCATTTAG GTGAGAACAT CGATAGCGCC 
GATGCTTTGT GGGGTGTGCA GTTGAAGCCT GTGGCTGGTG GCGGCAGCAG CGCAGCGCGT 
GAGAAAGAAG ACTCACCCAC TGCTGTGTTG AATAAATCCG TTGCGGAACG CGTGCGCATT 
GTGGAGAAAC GTCATGAGCA TAACGCTCCA AATGGTTACT CTACGAAAAT CAACTTGAGT 
CTGGACGAAC CAGCTGCAGC AGCGCGTGAT TGGCCTAATG CAGGTGCGCC TATGCTAATG 
CCAAACACTG ACAATCAACC CATACCCAAC AACACAAATC GCCGTGCGAG CGCACAAGAG 
CTCATATTAC AAGATCTACG CGAACATCAG CGTATGCTCG ACGAGGGACT CAAGTCTACA 
TCGCTCATCA TCAAAACAAT GCGTTCGGCA AATGAGTTTG ATGAGGCTAT GCGGCGTCTA 
AGTATAGCTT CACTGGAATC GAGTACGATG CCACCAATTG TGGTGCCCAC ACCAATGTTG 
CGTTCGAATA GCTACCAGGA GACGGTACGT CGTTACTCCA ACGCCTCCTT GGAGAGCACC 
GAACGCGAAC TTACCACACA GACTGTAGTT CATACGAGTA GCAGTGCTGC AGCGTTAACA 
GCGGCCACAA GTAGTCGCAG ATTGTCGTTT GAAACACAAA GCGCTTTCGT AACACCGTTA 
AGCCTACCAC CAGCACCGCT AGTGGCACCA CGTCTAAAGC TGCTCGGCAG CACTGAGGTA 
TCAGTGAGTC AGCAGCTTTC ACAGTTGCGA CGCATGTACG ATATTGCAGC GACACAGCAA 
GAAAATGACG AAGATGCCGC CGACAGTGCC GATGAAGAGG TAAAAAGCTA CTTTTGCGCA 
CACGAACAAG CGGAACAAAG CGATAGTGGT GGCGGCACGT TGGAGAGCTC TTCGCAGGAA 
GATGAGCGCG CCATTGAATA CTCCAGCGGC AGTTGGAGTC GCATGAAGGC GAAGCGCACA 
ATTTGGAAGA TTGAAGCGGA AGAACGTAAA GCGGCGCCGG CCGTATTGGA CAAAGCAGAT 
CTACCTAAAT CGAACATTAT GAGCATAGAA CTACATTCAC CACAAACTTC GTTGGAGAAA 
CGTGATTCCA TCAAACGCAA CCAAACACCG CCCGTGGCCA AACCGAGAAC TGTGACTGGT 
GCAGTCAACG CCACTCAGCA TCCACAGATA CATGTGGCCA CCACAGTCAA GCAAACAGCT 
GATCGTTTTT CAGCAGCCGA ACAGCAACGC CAGTCACTGA AAATTGTCAA GGAAGCGCGT 
GGTGCACGTA AACTGCGTGA ACACGAACTC TCATACTTTG GCGTACCACA CGGACAGAAA 
CACGAAGAGA ACGAAACAAA ACCGAAGCAA CAGCCGAATA GCAACGCTAA TACGTTGAGT 
TTATCGAAGC GTTCGCTACC GTCCCGTCAA CGTTTAACTG TTATTGGTAA CGAGGATACG 
AAATCGATGC GTCGACATGA AGCAAACAAC ACTGAAACAA CAACCGTAAG CGGACAAACA 
CAAACGGCCA CTAAATGGCA ATTGTTGAAT GACAAACCCG ATCTACTACG ACACAGTCCG 
ATTGCTGTGG AACCACAATT GATACGACGT ACAGCAGATG TGCAGCGTAG TGAACGTAAT 
AGCACATCCG TGGAACGTAA CAAACGTATG CCCTCAAAAG AGTTGAGGTT AGATGATGAG 
CTCAATGATG CTGTTGAAGA TGATGATGAC GACAACGAGC ATTTTTATGA AAATATAACA 
AATGAAATTA CGCCTGTCTT CAAAGTGAAG TCACCGCAAC CGTATGATCG TAAAATGGAT 
ATGGAACGCG ACGCATTCAT ATTGAACGAG ATGAATGAGA ATGCCGATCT GACTATGAAG 
GCGCTCTCCG ATGAGGCCGC CCATAAAGAT CGGCGACGAC GACGTTCATC GCTCCATAGA 
CGCGACTCCA AACCACTAGA AACGATCGAA GAGAAATTGG TCACCCAAAA ACCCAACGAC 
ACATGCATTG CGGTGAAAAT GTCACGTGGC ACGCTAGCCT CCGAAGCCAA ACGCCACGCA 
CGTCACACAA CCACTTCACC CAGCCCACAC GATACAACCA CACGAGTGCG TACTTCATCA 
CAATCGTCGG TAGAAAGTTG TCCGCGTGCA CGTTCGCGTT CACTCTCCAG CGAACGTGAG 
TACGAAAACA TACGTACACC GAAACCGACG CGCACCAACG CAACAGCCAC AACGAAAATA 
CAAGCCAAAA GCGTTAGCAA ACAACAGCAG CGCTCCAGTT CACGTGAATC ACAGCGTTCA 
GCGCGTTTGC CATATTCATC GGGCGATGAG GTAAACCCAC GCGAGGAACA ACGTGTACGC 
ATGAAGAGTA AACGTAGCTC GAACTCTACG AGTGGCACAA CGGGTCGTAG TGGTGAACGA 
CAACGTAGCA GTAAGAAGGA TAGCCATCAC GACAAACATG GACATCACAC GACAACAACA 
AGCAGTCGAG ATGTTGAGCA GCGACGGCGC GCGGCCAGCA GCACTACGCA TGCCTCTCAG 
CGAGAGGAAC GCGGTGAGTG CGTGACTGTG CCGCAACAAC AGCGCACCGC AAGTAGTGGA 
CGCAGCAGTA GCAGCAACAA GCGACGTGAA GAACCTGCCA CCACCGCTGG CAGTAGCGGA 
CGTCACTCCA GCTCACGCAA TGCCACGGAG AAGGCACGCA CTAGCGCTGG TGTGCGTCTA 
TTGCGCTCGC TACGCGTCGG ACGTACGGAT GATGCCAAAG CGTCCAGCAC GGAGCAACGT 
GCGTCCGGAC ATCATCGTTC CACCAGCGAG CGCGAGAAGG CACATGAGAA AGAGCGCGAA 
AAGGAGTTGC AGTCCAAATC GCGAGGTCAC TCAACCAGCA AAACCCAGCA CCACAGCAGT 
AGTGAGCACC ACCAACATTC ACTAACCGCA CATAGCGGTA CAATGTCCAG TAAAACACGC 
GAACACAAGA AAGCAACCTC AAACGCCATT GAGAAGCCGC ACGAACGAGC CATCACACCA 
ACGTACGTAT ACGAAACCGA AACGAATTTA CCACATATAC AAACCGATAG CAACAGTAGT 
GAGAGTTTAG TACAGACGCA AACCACAATT GCTGCTATGA CGCACAGCAC AACACATGAA 
TGTCACGAAA ATTATGCGCG ACTGAAGTCA GAGCTTAAAA CGCATACAAA TGCTGCGACA 
CAACATGCAA GCTTAACCGC AACACAATAT GACTATTGTA CGGAGGTGGC ACCATTACCA 
CCGCCGCGCA CACGCCGCAA GCGTCATAGA TCACTCATAC CTATACCCAT AAATCCTAGT 
ACACCAACGC CAACAGCAAC AACATCCCCA TCTCCATCCT CGTTGCCCAT TACATATGAA 
CATCAGATTT ATGCACAAAT CATACCGCCT GTTGCGTATG CACCCGCACA ACCCACATAT 
TCCAATCAGT CGACATTACG TAAATGTTCA TTGCAACGCA TTATGCGTAG ACGTAGCGCT 
TTACCCTTGC CCCTCACATT CCCATGTGCG CGCAAACCAT CGCAAACACA ATCGACCACC 
TCCTCATTCG CATTCCTACC CTATTTACCA GCCGCGCCCG GTAAGCGGCC CTCAGACGCG 
AGTCATATTT ATCATAATTA TTTATTGTAT GCAACGCCGG CGAAAGTTGA AGCAGAGCCG 
TTGAATTTGA CGCAACCAGC CGATGAGTTG GCGACATTTT GGCACGCCGA TGCGCTACAC 
CGACCGGAGC GGCCGCAGCG TTTGCGCACA ATGCGTAGTC ATGTGTCGAC GCATCATCCG 
TTCGCATTGT GCACATGCTC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176666.1
CDS	950..7393
Translation	MEVATTNTHHHNHHHQHLGHQLHHPAHRLPMAKSTQHQQQQQQSNHCLTVSNDSAQSGGGGGGGSPRSPFQREVREWQRIYPDTGALLSGRLEADRWINGPLNSYGKKSSSGGSSSSNNSTKLNTHKSAGGKVSLHAIVGSAGDNNTNSGSASRINNDLTESPKSTTTTAKSSNEALSGTKTWQQQQRRSNARHLEQATLYGQNSNSSKMRVGGADCDDCYAVVSADADESQLRYHQHHLQPQQLRKQQQQQQQSSVVLSETTPPTRRRDLESFRLYGDSTTAATTAGAAADDNAKADDLRLSTRQLRRQQQPFGQLSSSALIQRSHSISTDDLSNEWESNEAVAESNEWRRVSKLRRSFQSSSTANNSNTSAPAVQQPTSSIRRSYDLPVSAVSVSRIRAELESGRRLTTAMRNNHVDLAALESILHGPGKTTDTSSRNTLLTAESLKEIRGRLKKLSDESLYKDDFIAYQQPPSADEHIEVQQSVRRKLSTPRLQQLTPEESPEISTPTQHTSAFKVVGAQRKPTDNDSLTQRSTHLNQKHTSNSLESRQKQRDTNSTEWHSRRKSYGFEKMSPPDNKTMHRMDASTDSGLGRSGELGNWSPTEAAPPRATVIHFGEPAKAVTSAHYRAAKSPVRRTPDEDLLKRHSIAVDESQYVRDNLRKTSQVHLNGFYDPAEPAAKSTVQLSRYDSNAESNQLFQRNTQNAQKRVEFCKTEVHFAAESGRVNIVETDGKPPPTNNFRRRRRTTSGPLQSLTKTASTAGTSTAVSTTTASNVTHFGDDQHQQQRHRTIASSVVAYKATLVEPPTVLNDAQIMPATTNVTVSVQHAEGSRGSYASTTSGVDTTDNENDEIAAIRGILKNKPVKPKPYHLGENIDSADALWGVQLKPVAGGGSSAAREKEDSPTAVLNKSVAERVRIVEKRHEHNAPNGYSTKINLSLDEPAAAARDWPNAGAPMLMPNTDNQPIPNNTNRRASAQELILQDLREHQRMLDEGLKSTSLIIKTMRSANEFDEAMRRLSIASLESSTMPPIVVPTPMLRSNSYQETVRRYSNASLESTERELTTQTVVHTSSSAAALTAATSSRRLSFETQSAFVTPLSLPPAPLVAPRLKLLGSTEVSVSQQLSQLRRMYDIAATQQENDEDAADSADEEVKSYFCAHEQAEQSDSGGGTLESSSQEDERAIEYSSGSWSRMKAKRTIWKIEAEERKAAPAVLDKADLPKSNIMSIELHSPQTSLEKRDSIKRNQTPPVAKPRTVTGAVNATQHPQIHVATTVKQTADRFSAAEQQRQSLKIVKEARGARKLREHELSYFGVPHGQKHEENETKPKQQPNSNANTLSLSKRSLPSRQRLTVIGNEDTKSMRRHEANNTETTTVSGQTQTATKWQLLNDKPDLLRHSPIAVEPQLIRRTADVQRSERNSTSVERNKRMPSKELRLDDELNDAVEDDDDDNEHFYENITNEITPVFKVKSPQPYDRKMDMERDAFILNEMNENADLTMKALSDEAAHKDRRRRRSSLHRRDSKPLETIEEKLVTQKPNDTCIAVKMSRGTLASEAKRHARHTTTSPSPHDTTTRVRTSSQSSVESCPRARSRSLSSEREYENIRTPKPTRTNATATTKIQAKSVSKQQQRSSSRESQRSARLPYSSGDEVNPREEQRVRMKSKRSSNSTSGTTGRSGERQRSSKKDSHHDKHGHHTTTTSSRDVEQRRRAASSTTHASQREERGECVTVPQQQRTASSGRSSSSNKRREEPATTAGSSGRHSSSRNATEKARTSAGVRLLRSLRVGRTDDAKASSTEQRASGHHRSTSEREKAHEKEREKELQSKSRGHSTSKTQHHSSSEHHQHSLTAHSGTMSSKTREHKKATSNAIEKPHERAITPTYVYETETNLPHIQTDSNSSESLVQTQTTIAAMTHSTTHECHENYARLKSELKTHTNAATQHASLTATQYDYCTEVAPLPPPRTRRKRHRSLIPIPINPSTPTPTATTSPSPSSLPITYEHQIYAQIIPPVAYAPAQPTYSNQSTLRKCSLQRIMRRRSALPLPLTFPCARKPSQTQSTTSSFAFLPYLPAAPGKRPSDASHIYHNYLLYATPAKVEAEPLNLTQPADELATFWHADALHRPERPQRLRTMRSHVSTHHPFALCTCS

Target ORF information:

RefSeq Version	XM_011178364.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178364.1

ORF Insert Sequence:

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GAAAAGCAGC AGCGGCGGCA GCAGCAGCAG CAACAACTCC 
ACAAAGCTGA ACACCCACAA GAGTGCTGGC GGTAAGGTGA GCCTACATGC AATTGTTGGC 
AGCGCTGGTG ACAACAACAC CAACAGCGGC AGCGCTAGTC GAATCAACAA CGACTTAACA 
GAGTCACCAA AAAGCACAAC AACAACAGCG AAAAGTTCGA ATGAAGCATT GAGTGGTACA 
AAAACGTGGC AGCAGCAGCA GCGGCGTAGC AACGCTCGTC ATTTGGAACA AGCTACTCTA 
TATGGCCAAA ACAGCAATTC ATCTAAAATG CGCGTAGGCG GCGCTGATTG TGATGATTGT 
TATGCGGTTG TGTCGGCTGA CGCAGATGAG TCGCAGCTGC GCTACCACCA ACACCACCTT 
CAACCGCAAC AGCTACGTAA ACAACAACAA CAGCAGCAAC AAAGCAGTGT TGTGCTAAGT 
GAAACGACAC CACCAACGCG TCGACGTGAC TTGGAAAGTT TCCGGCTTTA CGGTGACTCC 
ACAACAGCAG CGACGACAGC GGGCGCGGCA GCGGATGACA ACGCCAAAGC CGATGATTTA 
CGTCTATCCA CGCGTCAATT ACGTCGACAA CAACAACCAT TTGGTCAACT GTCCAGCAGC 
GCTTTAATCC AACGTTCCCA TTCAATTTCG ACGGACGATC TCAGTAACGA GTGGGAGTCG 
AACGAGGCGG TTGCTGAGTC CAACGAGTGG CGACGCGTCA GCAAATTGCG ACGCTCCTTT 
CAATCATCCT CTACGGCGAA CAATAGTAAT ACCAGCGCTC CCGCAGTGCA ACAACCGACG 
AGCTCTATAC GCCGTTCATA CGATCTGCCC GTCAGTGCTG TGAGCGTGTC ACGCATACGC 
GCCGAACTCG AAAGCGGTCG ACGTCTTACC ACAGCAATGC GTAATAATCA TGTGGATTTG 
GCTGCACTAG AGAGTATACT ACACGGTCCA GGCAAAACTA CGGACACCAG TAGCCGTAAT 
ACATTGCTTA CCGCCGAGTC ATTGAAAGAA ATACGTGGCC GTCTCAAGAA ATTATCGGAT 
GAATCATTGT ATAAGGACGA TTTTATAGCA TATCAACAAC CGCCGTCAGC AGACGAGCAC 
ATTGAAGTGC AGCAGAGCGT GCGTCGTAAA CTGTCCACAC CACGTTTGCA ACAATTGACA 
CCGGAAGAGT CGCCCGAGAT CTCAACGCCA ACACAGCATA CATCTGCCTT CAAAGTTGTG 
GGCGCACAAC GCAAGCCAAC AGACAACGAC AGCTTAACGC AGCGCTCAAC GCACTTGAAC 
CAAAAACACA CCTCAAACAG CTTGGAATCA CGTCAAAAGC AAAGAGATAC CAATTCAACC 
GAGTGGCACT CGCGTCGCAA ATCATATGGT TTCGAGAAGA TGTCACCGCC GGACAACAAA 
ACTATGCACC GCATGGACGC ATCCACCGAT AGCGGACTCG GACGTTCCGG CGAACTGGGC 
AATTGGTCAC CCACGGAAGC AGCACCACCG CGCGCCACAG TCATACATTT CGGCGAGCCC 
GCAAAAGCAG TCACTTCGGC GCACTACCGC GCCGCTAAAT CCCCAGTGCG CCGCACACCT 
GACGAGGACT TGCTCAAGCG ACACTCGATC GCTGTCGATG AGAGTCAATA TGTGCGCGAT 
AATTTGCGTA AAACCTCACA GGTGCATTTG AATGGTTTCT ATGATCCGGC CGAGCCCGCT 
GCCAAATCCA CAGTGCAGTT GAGCCGTTAT GACAGCAATG CGGAGAGTAA TCAACTCTTC 
CAACGCAACA CACAAAATGC ACAGAAACGT GTTGAGTTCT GCAAAACCGA GGTACATTTC 
GCCGCCGAAT CGGGGCGTGT CAATATCGTT GAAACCGATG GTAAACCGCC ACCGACAAAT 
AATTTCCGTC GACGCCGTCG CACCACAAGT GGACCGCTAC AGAGTCTTAC CAAAACCGCA 
AGCACGGCGG GTACAAGCAC AGCCGTGAGC ACAACAACCG CTAGTAATGT TACACATTTC 
GGTGATGATC AACATCAGCA GCAACGTCAT CGCACCATCG CCAGTTCGGT GGTCGCATAT 
AAAGCTACAT TAGTGGAACC ACCAACTGTG CTCAATGACG CACAAATAAT GCCGGCCACA 
ACTAACGTAA CGGTATCCGT ACAACATGCG GAGGGTTCAC GTGGCAGCTA CGCGTCAACA 
ACAAGCGGTG TCGATACGAC AGACAATGAA AATGACGAAA TCGCCGCTAT ACGCGGCATA 
CTCAAGAATA AACCGGTAAA GCCGAAACCT TATCATTTAG GTGAGAACAT CGATAGCGCC 
GATGCTTTGT GGGGTGTGCA GTTGAAGCCT GTGGCTGGTG GCGGCAGCAG CGCAGCGCGT 
GAGAAAGAAG ACTCACCCAC TGCTGTGTTG AATAAATCCG TTGCGGAACG CGTGCGCATT 
GTGGAGAAAC GTCATGAGCA TAACGCTCCA AATGGTTACT CTACGAAAAT CAACTTGAGT 
CTGGACGAAC CAGCTGCAGC AGCGCGTGAT TGGCCTAATG CAGGTGCGCC TATGCTAATG 
CCAAACACTG ACAATCAACC CATACCCAAC AACACAAATC GCCGTGCGAG CGCACAAGAG 
CTCATATTAC AAGATCTACG CGAACATCAG CGTATGCTCG ACGAGGGACT CAAGTCTACA 
TCGCTCATCA TCAAAACAAT GCGTTCGGCA AATGAGTTTG ATGAGGCTAT GCGGCGTCTA 
AGTATAGCTT CACTGGAATC GAGTACGATG CCACCAATTG TGGTGCCCAC ACCAATGTTG 
CGTTCGAATA GCTACCAGGA GACGGTACGT CGTTACTCCA ACGCCTCCTT GGAGAGCACC 
GAACGCGAAC TTACCACACA GACTGTAGTT CATACGAGTA GCAGTGCTGC AGCGTTAACA 
GCGGCCACAA GTAGTCGCAG ATTGTCGTTT GAAACACAAA GCGCTTTCGT AACACCGTTA 
AGCCTACCAC CAGCACCGCT AGTGGCACCA CGTCTAAAGC TGCTCGGCAG CACTGAGGTA 
TCAGTGAGTC AGCAGCTTTC ACAGTTGCGA CGCATGTACG ATATTGCAGC GACACAGCAA 
GAAAATGACG AAGATGCCGC CGACAGTGCC GATGAAGAGG TAAAAAGCTA CTTTTGCGCA 
CACGAACAAG CGGAACAAAG CGATAGTGGT GGCGGCACGT TGGAGAGCTC TTCGCAGGAA 
GATGAGCGCG CCATTGAATA CTCCAGCGGC AGTTGGAGTC GCATGAAGGC GAAGCGCACA 
ATTTGGAAGA TTGAAGCGGA AGAACGTAAA GCGGCGCCGG CCGTATTGGA CAAAGCAGAT 
CTACCTAAAT CGAACATTAT GAGCATAGAA CTACATTCAC CACAAACTTC GTTGGAGAAA 
CGTGATTCCA TCAAACGCAA CCAAACACCG CCCGTGGCCA AACCGAGAAC TGTGACTGGT 
GCAGTCAACG CCACTCAGCA TCCACAGATA CATGTGGCCA CCACAGTCAA GCAAACAGCT 
GATCGTTTTT CAGCAGCCGA ACAGCAACGC CAGTCACTGA AAATTGTCAA GGAAGCGCGT 
GGTGCACGTA AACTGCGTGA ACACGAACTC TCATACTTTG GCGTACCACA CGGACAGAAA 
CACGAAGAGA ACGAAACAAA ACCGAAGCAA CAGCCGAATA GCAACGCTAA TACGTTGAGT 
TTATCGAAGC GTTCGCTACC GTCCCGTCAA CGTTTAACTG TTATTGGTAA CGAGGATACG 
AAATCGATGC GTCGACATGA AGCAAACAAC ACTGAAACAA CAACCGTAAG CGGACAAACA 
CAAACGGCCA CTAAATGGCA ATTGTTGAAT GACAAACCCG ATCTACTACG ACACAGTCCG 
ATTGCTGTGG AACCACAATT GATACGACGT ACAGCAGATG TGCAGCGTAG TGAACGTAAT 
AGCACATCCG TGGAACGTAA CAAACGTATG CCCTCAAAAG AGTTGAGGTT AGATGATGAG 
CTCAATGATG CTGTTGAAGA TGATGATGAC GACAACGAGC ATTTTTATGA AAATATAACA 
AATGAAATTA CGCCTGTCTT CAAAGTGAAG TCACCGCAAC CGTATGATCG TAAAATGGAT 
ATGGAACGCG ACGCATTCAT ATTGAACGAG ATGAATGAGA ATGCCGATCT GACTATGAAG 
GCGCTCTCCG ATGAGGCCGC CCATAAAGAT CGGCGACGAC GACGTTCATC GCTCCATAGA 
CGCGACTCCA AACCACTAGA AACGATCGAA GAGAAATTGG TCACCCAAAA ACCCAACGAC 
ACATGCATTG CGGTGAAAAT GTCACGTGGC ACGCTAGCCT CCGAAGCCAA ACGCCACGCA 
CGTCACACAA CCACTTCACC CAGCCCACAC GATACAACCA CACGAGTGCG TACTTCATCA 
CAATCGTCGG TAGAAAGTTG TCCGCGTGCA CGTTCGCGTT CACTCTCCAG CGAACGTGAG 
TACGAAAACA TACGTACACC GAAACCGACG CGCACCAACG CAACAGCCAC AACGAAAATA 
CAAGCCAAAA GCGTTAGCAA ACAACAGCAG CGCTCCAGTT CACGTGAATC ACAGCGTTCA 
GCGCGTTTGC CATATTCATC GGGCGATGAG GTAAACCCAC GCGAGGAACA ACGTGTACGC 
ATGAAGAGTA AACGTAGCTC GAACTCTACG AGTGGCACAA CGGGTCGTAG TGGTGAACGA 
CAACGTAGCA GTAAGAAGGA TAGCCATCAC GACAAACATG GACATCACAC GACAACAACA 
AGCAGTCGAG ATGTTGAGCA GCGACGGCGC GCGGCCAGCA GCACTACGCA TGCCTCTCAG 
CGAGAGGAAC GCGGTGAGTG CGTGACTGTG CCGCAACAAC AGCGCACCGC AAGTAGTGGA 
CGCAGCAGTA GCAGCAACAA GCGACGTGAA GAACCTGCCA CCACCGCTGG CAGTAGCGGA 
CGTCACTCCA GCTCACGCAA TGCCACGGAG AAGGCACGCA CTAGCGCTGG TGTGCGTCTA 
TTGCGCTCGC TACGCGTCGG ACGTACGGAT GATGCCAAAG CGTCCAGCAC GGAGCAACGT 
GCGTCCGGAC ATCATCGTTC CACCAGCGAG CGCGAGAAGG CACATGAGAA AGAGCGCGAA 
AAGGAGTTGC AGTCCAAATC GCGAGGTCAC TCAACCAGCA AAACCCAGCA CCACAGCAGT 
AGTGAGCACC ACCAACATTC ACTAACCGCA CATAGCGGTA CAATGTCCAG TAAAACACGC 
GAACACAAGA AAGCAACCTC AAACGCCATT GAGAAGCCGC ACGAACGAGC CATCACACCA 
ACGTACGTAT ACGAAACCGA AACGAATTTA CCACATATAC AAACCGATAG CAACAGTAGT 
GAGAGTTTAG TACAGACGCA AACCACAATT GCTGCTATGA CGCACAGCAC AACACATGAA 
TGTCACGAAA ATTATGCGCG ACTGAAGTCA GAGCTTAAAA CGCATACAAA TGCTGCGACA 
CAACATGCAA GCTTAACCGC AACACAATAT GACTATTGTA CGGAGGTGGC ACCATTACCA 
CCGCCGCGCA CACGCCGCAA GCGTCATAGA TCACTCATAC CTATACCCAT AAATCCTAGT 
ACACCAACGC CAACAGCAAC AACATCCCCA TCTCCATCCT CGTTGCCCAT TACATATGAA 
CATCAGATTT ATGCACAAAT CATACCGCCT GTTGCGTATG CACCCGCACA ACCCACATAT 
TCCAATCAGT CGACATTACG TAAATGTTCA TTGCAACGCA TTATGCGTAG ACGTAGCGCT 
TTACCCTTGC CCCTCACATT CCCATGTGCG CGCAAACCAT CGCAAACACA ATCGACCACC 
TCCTCATTCG CATTCCTACC CTATTTACCA GCCGCGCCCG GTAAGCGGCC CTCAGACGCG 
AGTCATATTT ATCATAATTA TTTATTGTAT GCAACGCCGG CGAAAGTTGA AGCAGAGCCG 
TTGAATTTGA CGCAACCAGC CGATGAGTTG GCGACATTTT GGCACGCCGA TGCGCTACAC 
CGACCGGAGC GGCCGCAGCG TTTGCGCACA ATGCGTAGTC ATGTGTCGAC GCATCATCCG 
TTCGCATTGT GCACATGCTC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208490 ( XM_029037938.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028893771.1 Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X4, mRNA.

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CloneID	OBa254487
Clone ID Related Accession (Same CDS sequence)	XM_029037938.1
Accession Version	XM_029037938.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105208490 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863673.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGTCA ACGCCACTCA GCATCCACAG 
ATACATGTGG CCACCACAGT CAAGCAAACA GCTGATCGTT TTTCAGCAGC CGAACAGCAA 
CGCCAGTCAC TGAAAATTGT CAAGGAAGCG CGTGGTGCAC GTAAACTGCG TGAACACGAA 
CTCTCATACT TTGGCGTACC ACACGGACAG AAACACGAAG AGAACGAAAC AAAACCGAAG 
CAACAGCCGA ATAGCAACGC TAATACGTTG AGTTTATCGA AGCGTTCGCT ACCGTCCCGT 
CAACGTTTAA CTGTTATTGG TAACGAGGAT ACGAAATCGA TGCGTCGACA TGAAGCAAAC 
AACACTGAAA CAACAACCGT AAGCGGACAA ACACAAACGG CCACTAAATG GCAATTGTTG 
AATGACAAAC CCGATCTACT ACGACACAGT CCGATTGCTG TGGAACCACA ATTGATACGA 
CGTACAGCAG ATGTGCAGCG TAGTGAACGT AATAGCACAT CCGTGGAACG TAACAAACGT 
ATGCCCTCAA AAGAGTTGAG GTTAGATGAT GAGCTCAATG ATGCTGTTGA AGATGATGAT 
GACGACAACG AGCATTTTTA TGAAAATATA ACAAATGAAA TTACGCCTGT CTTCAAAGTG 
AAGTCACCGC AACCGTATGA TCGTAAAATG GATATGGAAC GCGACGCATT CATATTGAAC 
GAGATGAATG AGAATGCCGA TCTGACTATG AAGGCGCTCT CCGATGAGGC CGCCCATAAA 
GATCGGCGAC GACGACGTTC ATCGCTCCAT AGACGCGACT CCAAACCACT AGAAACGATC 
GAAGAGAAAT TGGTCACCCA AAAACCCAAC GACACATGCA TTGCGGTGAA AATGTCACGT 
GGCACGCTAG CCTCCGAAGC CAAACGCCAC GCACGTCACA CAACCACTTC ACCCAGCCCA 
CACGATACAA CCACACGAGT GCGTACTTCA TCACAATCGT CGGTAGAAAG TTGTCCGCGT 
GCACGTTCGC GTTCACTCTC CAGCGAACGT GAGTACGAAA ACATACGTAC ACCGAAACCG 
ACGCGCACCA ACGCAACAGC CACAACGAAA ATACAAGCCA AAAGCGTTAG CAAACAACAG 
CAGCGCTCCA GTTCACGTGA ATCACAGCGT TCAGCGCGTT TGCCATATTC ATCGGGCGAT 
GAGGTAAACC CACGCGAGGA ACAACGTGTA CGCATGAAGA GTAAACGTAG CTCGAACTCT 
ACGAGTGGCA CAACGGGTCG TAGTGGTGAA CGACAACGTA GCAGTAAGAA GGATAGCCAT 
CACGACAAAC ATGGACATCA CACGACAACA ACAAGCAGTC GAGATGTTGA GCAGCGACGG 
CGCGCGGCCA GCAGCACTAC GCATGCCTCT CAGCGAGAGG AACGCGGTGA GTGCGTGACT 
GTGCCGCAAC AACAGCGCAC CGCAAGTAGT GGACGCAGCA GTAGCAGCAA CAAGCGACGT 
GAAGAACCTG CCACCACCGC TGGCAGTAGC GGACGTCACT CCAGCTCACG CAATGCCACG 
GAGAAGGCAC GCACTAGCGC TGGTGTGCGT CTATTGCGCT CGCTACGCGT CGGACGTACG 
GATGATGCCA AAGCGTCCAG CACGGAGCAA CGTGCGTCCG GACATCATCG TTCCACCAGC 
GAGCGCGAGA AGGCACATGA GAAAGAGCGC GAAAAGGAGT TGCAGTCCAA ATCGCGAGGT 
CACTCAACCA GCAAAACCCA GCACCACAGC AGTAGTGAGC ACCACCAACA TTCACTAACC 
GCACATAGCG GTACAATGTC CAGTAAAACA CGCGAACACA AGAAAGCAAC CTCAAACGCC 
ATTGAGAAGC CGCACGAACG AGCCATCACA CCAACACACA GTAAAAGCTC GCAAAACATA 
AATGGACGAC ATACTACTAC TGGACAACAT GATGGCCACC ACCGCAAACA TAACGATCAT 
AAACGAGCAC ACACACGACC ACAACAAGAA GAGAGCAGAA CAACGAAGAC AACGACGACG 
CAGCGAAAGC GTAGTGAACA TAGCGCAAGG GAGCGCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028893771.1
CDS	948..7469
Translation	MEVATTNTHHHNHHHQHLGHQLHHPAHRLPMAKSTQHQQQQQQSNHCLTVSNDSAQSGGGGGGGSPRSPFQREVREWQRIYPDTGALLSGRLEADRWINGPLNSYGKISDSQNISQPNGTQHTQRKQLEVLQKRTANGAVQVIRAQTVQTSSSRSWSSSNSNSASTSASASASASPICMQANVVGARSLLHNHTNNNINNINSSKHFHSTLQQHLAEANTHWLQLSPPTIAATVDISEVSTDDDLDKAVPTIAETPYIVDDSEIDVGNGCGVDADINASATLCNYSISRSGSAGSGGGGSSKGHALLSSAASSSSTLHSQSSALLVPDTAPSLKLSNLMKSSSGGSSSSNNSTKLNTHKSAGGKVSLHAIVGSAGDNNTNSGSASRINNDLTESPKSTTTTAKSSNEALSGTKTWQQQQRRSNARHLEQATLYGQNSNSSKMRVGGADCDDCYAVVSADADESQLRYHQHHLQPQQLRKQQQQQQQSSVVLSETTPPTRRRDLESFRLYGDSTTAATTAGAAADDNAKADDLRLSTRQLRRQQQPFGQLSSSALIQRSHSISTDDLSNEWESNEAVAESNEWRRVSKLRRSFQSSSTANNSNTSAPAVQQPTSSIRRSYDLPVSAVSVSRIRAELESGRRLTTAMRNNHVDLAALESILHGPGKTTDTSSRNTLLTAESLKEIRGRLKKLSDESLYKDDFIAYQQPPSADEHIEVQQSVRRKLSTPRLQQLTPEESPEISTPTQHTSAFKVVGAQRKPTDNDSLTQRSTHLNQKHTSNSLESRQKQRDTNSTEWHSRRKSYGFEKMSPPDNKTMHRMDASTDSGLGRSGELGNWSPTEAAPPRATVIHFGEPAKAVTSAHYRAAKSPVRRTPDEDLLKRHSIAVDESQYVRDNLRKTSQVHLNGFYDPAEPAAKSTVQLSRYDSNAESNQLFQRNTQNAQKRVEFCKTEVHFAAESGRVNIVETDGKPPPTNNFRRRRRTTSGPLQSLTKTASTAGTSTAVSTTTASNVTHFGDDQHQQQRHRTIASSVVAYKATLVEPPTVLNDAQIMPATTNVTVSVQHAEGSRGSYASTTSGVDTTDNENDEIAAIRGILKNKPVKPKPYHLGENIDSADALWGVQLKPVAGGGSSAAREKEDSPTAVLNKSVAERVRIVEKRHEHNAPNGYSTKINLSLDEPAAAARDWPNAGAPMLMPNTDNQPIPNNTNRRASAQELILQDLREHQRMLDEGLKSTSLIIKTMRSANEFDEAMRRLSIASLESSTMPPIVVPTPMLRSNSYQETVRRYSNASLESTERELTTQTVVHTSSSAAALTAATSSRRLSFETQSAFVTPLSLPPAPLVAPRLKLLGSTEVSVSQQLSQLRRMYDIAATQQENDEDAADSADEEVKSYFCAHEQAEQSDSGGGTLESSSQEDERAIEYSSGSWSRMKAKRTIWKIEAEERKAAPAVLDKADLPKSNIMSIELHSPQTSLEKRDSIKRNQTPPVAKPRTVTGVNATQHPQIHVATTVKQTADRFSAAEQQRQSLKIVKEARGARKLREHELSYFGVPHGQKHEENETKPKQQPNSNANTLSLSKRSLPSRQRLTVIGNEDTKSMRRHEANNTETTTVSGQTQTATKWQLLNDKPDLLRHSPIAVEPQLIRRTADVQRSERNSTSVERNKRMPSKELRLDDELNDAVEDDDDDNEHFYENITNEITPVFKVKSPQPYDRKMDMERDAFILNEMNENADLTMKALSDEAAHKDRRRRRSSLHRRDSKPLETIEEKLVTQKPNDTCIAVKMSRGTLASEAKRHARHTTTSPSPHDTTTRVRTSSQSSVESCPRARSRSLSSEREYENIRTPKPTRTNATATTKIQAKSVSKQQQRSSSRESQRSARLPYSSGDEVNPREEQRVRMKSKRSSNSTSGTTGRSGERQRSSKKDSHHDKHGHHTTTTSSRDVEQRRRAASSTTHASQREERGECVTVPQQQRTASSGRSSSSNKRREEPATTAGSSGRHSSSRNATEKARTSAGVRLLRSLRVGRTDDAKASSTEQRASGHHRSTSEREKAHEKEREKELQSKSRGHSTSKTQHHSSSEHHQHSLTAHSGTMSSKTREHKKATSNAIEKPHERAITPTHSKSSQNINGRHTTTGQHDGHHRKHNDHKRAHTRPQQEESRTTKTTTTQRKRSEHSARERK

Target ORF information:

RefSeq Version	XM_029037938.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029037938.1

ORF Insert Sequence:

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGTCA ACGCCACTCA GCATCCACAG 
ATACATGTGG CCACCACAGT CAAGCAAACA GCTGATCGTT TTTCAGCAGC CGAACAGCAA 
CGCCAGTCAC TGAAAATTGT CAAGGAAGCG CGTGGTGCAC GTAAACTGCG TGAACACGAA 
CTCTCATACT TTGGCGTACC ACACGGACAG AAACACGAAG AGAACGAAAC AAAACCGAAG 
CAACAGCCGA ATAGCAACGC TAATACGTTG AGTTTATCGA AGCGTTCGCT ACCGTCCCGT 
CAACGTTTAA CTGTTATTGG TAACGAGGAT ACGAAATCGA TGCGTCGACA TGAAGCAAAC 
AACACTGAAA CAACAACCGT AAGCGGACAA ACACAAACGG CCACTAAATG GCAATTGTTG 
AATGACAAAC CCGATCTACT ACGACACAGT CCGATTGCTG TGGAACCACA ATTGATACGA 
CGTACAGCAG ATGTGCAGCG TAGTGAACGT AATAGCACAT CCGTGGAACG TAACAAACGT 
ATGCCCTCAA AAGAGTTGAG GTTAGATGAT GAGCTCAATG ATGCTGTTGA AGATGATGAT 
GACGACAACG AGCATTTTTA TGAAAATATA ACAAATGAAA TTACGCCTGT CTTCAAAGTG 
AAGTCACCGC AACCGTATGA TCGTAAAATG GATATGGAAC GCGACGCATT CATATTGAAC 
GAGATGAATG AGAATGCCGA TCTGACTATG AAGGCGCTCT CCGATGAGGC CGCCCATAAA 
GATCGGCGAC GACGACGTTC ATCGCTCCAT AGACGCGACT CCAAACCACT AGAAACGATC 
GAAGAGAAAT TGGTCACCCA AAAACCCAAC GACACATGCA TTGCGGTGAA AATGTCACGT 
GGCACGCTAG CCTCCGAAGC CAAACGCCAC GCACGTCACA CAACCACTTC ACCCAGCCCA 
CACGATACAA CCACACGAGT GCGTACTTCA TCACAATCGT CGGTAGAAAG TTGTCCGCGT 
GCACGTTCGC GTTCACTCTC CAGCGAACGT GAGTACGAAA ACATACGTAC ACCGAAACCG 
ACGCGCACCA ACGCAACAGC CACAACGAAA ATACAAGCCA AAAGCGTTAG CAAACAACAG 
CAGCGCTCCA GTTCACGTGA ATCACAGCGT TCAGCGCGTT TGCCATATTC ATCGGGCGAT 
GAGGTAAACC CACGCGAGGA ACAACGTGTA CGCATGAAGA GTAAACGTAG CTCGAACTCT 
ACGAGTGGCA CAACGGGTCG TAGTGGTGAA CGACAACGTA GCAGTAAGAA GGATAGCCAT 
CACGACAAAC ATGGACATCA CACGACAACA ACAAGCAGTC GAGATGTTGA GCAGCGACGG 
CGCGCGGCCA GCAGCACTAC GCATGCCTCT CAGCGAGAGG AACGCGGTGA GTGCGTGACT 
GTGCCGCAAC AACAGCGCAC CGCAAGTAGT GGACGCAGCA GTAGCAGCAA CAAGCGACGT 
GAAGAACCTG CCACCACCGC TGGCAGTAGC GGACGTCACT CCAGCTCACG CAATGCCACG 
GAGAAGGCAC GCACTAGCGC TGGTGTGCGT CTATTGCGCT CGCTACGCGT CGGACGTACG 
GATGATGCCA AAGCGTCCAG CACGGAGCAA CGTGCGTCCG GACATCATCG TTCCACCAGC 
GAGCGCGAGA AGGCACATGA GAAAGAGCGC GAAAAGGAGT TGCAGTCCAA ATCGCGAGGT 
CACTCAACCA GCAAAACCCA GCACCACAGC AGTAGTGAGC ACCACCAACA TTCACTAACC 
GCACATAGCG GTACAATGTC CAGTAAAACA CGCGAACACA AGAAAGCAAC CTCAAACGCC 
ATTGAGAAGC CGCACGAACG AGCCATCACA CCAACACACA GTAAAAGCTC GCAAAACATA 
AATGGACGAC ATACTACTAC TGGACAACAT GATGGCCACC ACCGCAAACA TAACGATCAT 
AAACGAGCAC ACACACGACC ACAACAAGAA GAGAGCAGAA CAACGAAGAC AACGACGACG 
CAGCGAAAGC GTAGTGAACA TAGCGCAAGG GAGCGCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208490 ( XM_029037939.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028893772.1 Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X5, mRNA.

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CloneID	OBa254488
Clone ID Related Accession (Same CDS sequence)	XM_029037939.1
Accession Version	XM_029037939.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105208490 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863673.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GAGCTGCTTG TTTTGGGCAT CTTAATAATG GGTGCTACAC AGCCGCAAAC ACTACACAAA 
ACTCTTAGAC ACAGTAAAAG CTCGCAAAAC ATAAATGGAC GACATACTAC TACTGGACAA 
CATGATGGCC ACCACCGCAA ACATAACGAT CATAAACGAG CACACACACG ACCACAACAA 
GAAGAGAGCA GAACAACGAA GACAACGACG ACGCAGCGAA AGCGTAGTGA ACATAGCGCA 
AGGGAGCGCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028893772.1
CDS	948..7382
Translation	MEVATTNTHHHNHHHQHLGHQLHHPAHRLPMAKSTQHQQQQQQSNHCLTVSNDSAQSGGGGGGGSPRSPFQREVREWQRIYPDTGALLSGRLEADRWINGPLNSYGKISDSQNISQPNGTQHTQRKQLEVLQKRTANGAVQVIRAQTVQTSSSRSWSSSNSNSASTSASASASASPICMQANVVGARSLLHNHTNNNINNINSSKHFHSTLQQHLAEANTHWLQLSPPTIAATVDISEVSTDDDLDKAVPTIAETPYIVDDSEIDVGNGCGVDADINASATLCNYSISRSGSAGSGGGGSSKGHALLSSAASSSSTLHSQSSALLVPDTAPSLKLSNLMKSSSGGSSSSNNSTKLNTHKSAGGKVSLHAIVGSAGDNNTNSGSASRINNDLTESPKSTTTTAKSSNEALSGTKTWQQQQRRSNARHLEQATLYGQNSNSSKMRVGGADCDDCYAVVSADADESQLRYHQHHLQPQQLRKQQQQQQQSSVVLSETTPPTRRRDLESFRLYGDSTTAATTAGAAADDNAKADDLRLSTRQLRRQQQPFGQLSSSALIQRSHSISTDDLSNEWESNEAVAESNEWRRVSKLRRSFQSSSTANNSNTSAPAVQQPTSSIRRSYDLPVSAVSVSRIRAELESGRRLTTAMRNNHVDLAALESILHGPGKTTDTSSRNTLLTAESLKEIRGRLKKLSDESLYKDDFIAYQQPPSADEHIEVQQSVRRKLSTPRLQQLTPEESPEISTPTQHTSAFKVVGAQRKPTDNDSLTQRSTHLNQKHTSNSLESRQKQRDTNSTEWHSRRKSYGFEKMSPPDNKTMHRMDASTDSGLGRSGELGNWSPTEAAPPRATVIHFGEPAKAVTSAHYRAAKSPVRRTPDEDLLKRHSIAVDESQYVRDNLRKTSQVHLNGFYDPAEPAAKSTVQLSRYDSNAESNQLFQRNTQNAQKRVEFCKTEVHFAAESGRVNIVETDGKPPPTNNFRRRRRTTSGPLQSLTKTASTAGTSTAVSTTTASNVTHFGDDQHQQQRHRTIASSVVAYKATLVEPPTVLNDAQIMPATTNVTVSVQHAEGSRGSYASTTSGVDTTDNENDEIAAIRGILKNKPVKPKPYHLGENIDSADALWGVQLKPVAGGGSSAAREKEDSPTAVLNKSVAERVRIVEKRHEHNAPNGYSTKINLSLDEPAAAARDWPNAGAPMLMPNTDNQPIPNNTNRRASAQELILQDLREHQRMLDEGLKSTSLIIKTMRSANEFDEAMRRLSIASLESSTMPPIVVPTPMLRSNSYQETVRRYSNASLESTERELTTQTVVHTSSSAAALTAATSSRRLSFETQSAFVTPLSLPPAPLVAPRLKLLGSTEVSVSQQLSQLRRMYDIAATQQENDEDAADSADEEVKSYFCAHEQAEQSDSGGGTLESSSQEDERAIEYSSGSWSRMKAKRTIWKIEAEERKAAPAVLDKADLPKSNIMSIELHSPQTSLEKRDSIKRNQTPPVAKPRTVTGAVNATQHPQIHVATTVKQTADRFSAAEQQRQSLKIVKEARGARKLREHELSYFGVPHGQKHEENETKPKQQPNSNANTLSLSKRSLPSRQRLTVIGNEDTKSMRRHEANNTETTTVSGQTQTATKWQLLNDKPDLLRHSPIAVEPQLIRRTADVQRSERNSTSVERNKRMPSKELRLDDELNDAVEDDDDDNEHFYENITNEITPVFKVKSPQPYDRKMDMERDAFILNEMNENADLTMKALSDEAAHKDRRRRRSSLHRRDSKPLETIEEKLVTQKPNDTCIAVKMSRGTLASEAKRHARHTTTSPSPHDTTTRVRTSSQSSVESCPRARSRSLSSEREYENIRTPKPTRTNATATTKIQAKSVSKQQQRSSSRESQRSARLPYSSGDEVNPREEQRVRMKSKRSSNSTSGTTGRSGERQRSSKKDSHHDKHGHHTTTTSSRDVEQRRRAASSTTHASQREERGECVTVPQQQRTASSGRSSSSNKRREEPATTAGSSGRHSSSRNATEKARTSAGVRLLRSLRVGRTDDAKASSTEQRASGHHRSTSEREKAHEKEREKELQSKSRELLVLGILIMGATQPQTLHKTLRHSKSSQNINGRHTTTGQHDGHHRKHNDHKRAHTRPQQEESRTTKTTTTQRKRSEHSARERK

Target ORF information:

RefSeq Version	XM_029037939.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029037939.1

ORF Insert Sequence:

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GAGCTGCTTG TTTTGGGCAT CTTAATAATG GGTGCTACAC AGCCGCAAAC ACTACACAAA 
ACTCTTAGAC ACAGTAAAAG CTCGCAAAAC ATAAATGGAC GACATACTAC TACTGGACAA 
CATGATGGCC ACCACCGCAA ACATAACGAT CATAAACGAG CACACACACG ACCACAACAA 
GAAGAGAGCA GAACAACGAA GACAACGACG ACGCAGCGAA AGCGTAGTGA ACATAGCGCA 
AGGGAGCGCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208490 ( XM_011178360.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176662.1 Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X2, mRNA.

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CloneID	OBa194180
Clone ID Related Accession (Same CDS sequence)	XM_011178360.1 , XM_011178362.1 , XM_011178359.1
Accession Version	XM_011178360.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105208490 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863673.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GGTCACTCAA CCAGCAAAAC CCAGCACCAC AGCAGTAGTG AGCACCACCA ACATTCACTA 
ACCGCACATA GCGGTACAAT GTCCAGTAAA ACACGCGAAC ACAAGAAAGC AACCTCAAAC 
GCCATTGAGA AGCCGCACGA ACGAGCCATC ACACCAACGT ACGTATACGA AACCGAAACG 
AATTTACCAC ATATACAAAC CGATAGCAAC AGTAGTGAGA GTTTAGTACA GACGCAAACC 
ACAATTGCTG CTATGACGCA CAGCACAACA CATGAATGTC ACGAAAATTA TGCGCGACTG 
AAGTCAGAGC TTAAAACGCA TACAAATGCT GCGACACAAC ATGCAAGCTT AACCGCAACA 
CAATATGACT ATTGTACGGA GGTGGCACCA TTACCACCGC CGCGCACACG CCGCAAGCGT 
CATAGATCAC TCATACCTAT ACCCATAAAT CCTAGTACAC CAACGCCAAC AGCAACAACA 
TCCCCATCTC CATCCTCGTT GCCCATTACA TATGAACATC AGATTTATGC ACAAATCATA 
CCGCCTGTTG CGTATGCACC CGCACAACCC ACATATTCCA ATCAGTCGAC ATTACGTAAA 
TGTTCATTGC AACGCATTAT GCGTAGACGT AGCGCTTTAC CCTTGCCCCT CACATTCCCA 
TGTGCGCGCA AACCATCGCA AACACAATCG ACCACCTCCT CATTCGCATT CCTACCCTAT 
TTACCAGCCG CGCCCGGTAA GCGGCCCTCA GACGCGAGTC ATATTTATCA TAATTATTTA 
TTGTATGCAA CGCCGGCGAA AGTTGAAGCA GAGCCGTTGA ATTTGACGCA ACCAGCCGAT 
GAGTTGGCGA CATTTTGGCA CGCCGATGCG CTACACCGAC CGGAGCGGCC GCAGCGTTTG 
CGCACAATGC GTAGTCATGT GTCGACGCAT CATCCGTTCG CATTGTGCAC ATGCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176662.1
CDS	1159..8298
Translation	MEVATTNTHHHNHHHQHLGHQLHHPAHRLPMAKSTQHQQQQQQSNHCLTVSNDSAQSGGGGGGGSPRSPFQREVREWQRIYPDTGALLSGRLEADRWINGPLNSYGKISDSQNISQPNGTQHTQRKQLEVLQKRTANGAVQVIRAQTVQTSSSRSWSSSNSNSASTSASASASASPICMQANVVGARSLLHNHTNNNINNINSSKHFHSTLQQHLAEANTHWLQLSPPTIAATVDISEVSTDDDLDKAVPTIAETPYIVDDSEIDVGNGCGVDADINASATLCNYSISRSGSAGSGGGGSSKGHALLSSAASSSSTLHSQSSALLVPDTAPSLKLSNLMKSSSGGSSSSNNSTKLNTHKSAGGKVSLHAIVGSAGDNNTNSGSASRINNDLTESPKSTTTTAKSSNEALSGTKTWQQQQRRSNARHLEQATLYGQNSNSSKMRVGGADCDDCYAVVSADADESQLRYHQHHLQPQQLRKQQQQQQQSSVVLSETTPPTRRRDLESFRLYGDSTTAATTAGAAADDNAKADDLRLSTRQLRRQQQPFGQLSSSALIQRSHSISTDDLSNEWESNEAVAESNEWRRVSKLRRSFQSSSTANNSNTSAPAVQQPTSSIRRSYDLPVSAVSVSRIRAELESGRRLTTAMRNNHVDLAALESILHGPGKTTDTSSRNTLLTAESLKEIRGRLKKLSDESLYKDDFIAYQQPPSADEHIEVQQSVRRKLSTPRLQQLTPEESPEISTPTQHTSAFKVVGAQRKPTDNDSLTQRSTHLNQKHTSNSLESRQKQRDTNSTEWHSRRKSYGFEKMSPPDNKTMHRMDASTDSGLGRSGELGNWSPTEAAPPRATVIHFGEPAKAVTSAHYRAAKSPVRRTPDEDLLKRHSIAVDESQYVRDNLRKTSQVHLNGFYDPAEPAAKSTVQLSRYDSNAESNQLFQRNTQNAQKRVEFCKTEVHFAAESGRVNIVETDGKPPPTNNFRRRRRTTSGPLQSLTKTASTAGTSTAVSTTTASNVTHFGDDQHQQQRHRTIASSVVAYKATLVEPPTVLNDAQIMPATTNVTVSVQHAEGSRGSYASTTSGVDTTDNENDEIAAIRGILKNKPVKPKPYHLGENIDSADALWGVQLKPVAGGGSSAAREKEDSPTAVLNKSVAERVRIVEKRHEHNAPNGYSTKINLSLDEPAAAARDWPNAGAPMLMPNTDNQPIPNNTNRRASAQELILQDLREHQRMLDEGLKSTSLIIKTMRSANEFDEAMRRLSIASLESSTMPPIVVPTPMLRSNSYQETVRRYSNASLESTERELTTQTVVHTSSSAAALTAATSSRRLSFETQSAFVTPLSLPPAPLVAPRLKLLGSTEVSVSQQLSQLRRMYDIAATQQENDEDAADSADEEVKSYFCAHEQAEQSDSGGGTLESSSQEDERAIEYSSGSWSRMKAKRTIWKIEAEERKAAPAVLDKADLPKSNIMSIELHSPQTSLEKRDSIKRNQTPPVAKPRTVTGAVNATQHPQIHVATTVKQTADRFSAAEQQRQSLKIVKEARGARKLREHELSYFGVPHGQKHEENETKPKQQPNSNANTLSLSKRSLPSRQRLTVIGNEDTKSMRRHEANNTETTTVSGQTQTATKWQLLNDKPDLLRHSPIAVEPQLIRRTADVQRSERNSTSVERNKRMPSKELRLDDELNDAVEDDDDDNEHFYENITNEITPVFKVKSPQPYDRKMDMERDAFILNEMNENADLTMKALSDEAAHKDRRRRRSSLHRRDSKPLETIEEKLVTQKPNDTCIAVKMSRGTLASEAKRHARHTTTSPSPHDTTTRVRTSSQSSVESCPRARSRSLSSEREYENIRTPKPTRTNATATTKIQAKSVSKQQQRSSSRESQRSARLPYSSGDEVNPREEQRVRMKSKRSSNSTSGTTGRSGERQRSSKKDSHHDKHGHHTTTTSSRDVEQRRRAASSTTHASQREERGECVTVPQQQRTASSGRSSSSNKRREEPATTAGSSGRHSSSRNATEKARTSAGVRLLRSLRVGRTDDAKASSTEQRASGHHRSTSEREKAHEKEREKELQSKSRGHSTSKTQHHSSSEHHQHSLTAHSGTMSSKTREHKKATSNAIEKPHERAITPTYVYETETNLPHIQTDSNSSESLVQTQTTIAAMTHSTTHECHENYARLKSELKTHTNAATQHASLTATQYDYCTEVAPLPPPRTRRKRHRSLIPIPINPSTPTPTATTSPSPSSLPITYEHQIYAQIIPPVAYAPAQPTYSNQSTLRKCSLQRIMRRRSALPLPLTFPCARKPSQTQSTTSSFAFLPYLPAAPGKRPSDASHIYHNYLLYATPAKVEAEPLNLTQPADELATFWHADALHRPERPQRLRTMRSHVSTHHPFALCTCS

Target ORF information:

RefSeq Version	XM_011178360.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178360.1

ORF Insert Sequence:

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GGTCACTCAA CCAGCAAAAC CCAGCACCAC AGCAGTAGTG AGCACCACCA ACATTCACTA 
ACCGCACATA GCGGTACAAT GTCCAGTAAA ACACGCGAAC ACAAGAAAGC AACCTCAAAC 
GCCATTGAGA AGCCGCACGA ACGAGCCATC ACACCAACGT ACGTATACGA AACCGAAACG 
AATTTACCAC ATATACAAAC CGATAGCAAC AGTAGTGAGA GTTTAGTACA GACGCAAACC 
ACAATTGCTG CTATGACGCA CAGCACAACA CATGAATGTC ACGAAAATTA TGCGCGACTG 
AAGTCAGAGC TTAAAACGCA TACAAATGCT GCGACACAAC ATGCAAGCTT AACCGCAACA 
CAATATGACT ATTGTACGGA GGTGGCACCA TTACCACCGC CGCGCACACG CCGCAAGCGT 
CATAGATCAC TCATACCTAT ACCCATAAAT CCTAGTACAC CAACGCCAAC AGCAACAACA 
TCCCCATCTC CATCCTCGTT GCCCATTACA TATGAACATC AGATTTATGC ACAAATCATA 
CCGCCTGTTG CGTATGCACC CGCACAACCC ACATATTCCA ATCAGTCGAC ATTACGTAAA 
TGTTCATTGC AACGCATTAT GCGTAGACGT AGCGCTTTAC CCTTGCCCCT CACATTCCCA 
TGTGCGCGCA AACCATCGCA AACACAATCG ACCACCTCCT CATTCGCATT CCTACCCTAT 
TTACCAGCCG CGCCCGGTAA GCGGCCCTCA GACGCGAGTC ATATTTATCA TAATTATTTA 
TTGTATGCAA CGCCGGCGAA AGTTGAAGCA GAGCCGTTGA ATTTGACGCA ACCAGCCGAT 
GAGTTGGCGA CATTTTGGCA CGCCGATGCG CTACACCGAC CGGAGCGGCC GCAGCGTTTG 
CGCACAATGC GTAGTCATGT GTCGACGCAT CATCCGTTCG CATTGTGCAC ATGCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208490 ( XM_011178362.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176664.1 Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X3, mRNA.

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CloneID	OBa194180
Clone ID Related Accession (Same CDS sequence)	XM_011178360.1 , XM_011178362.1 , XM_011178359.1
Accession Version	XM_011178362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105208490 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863673.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GGTCACTCAA CCAGCAAAAC CCAGCACCAC AGCAGTAGTG AGCACCACCA ACATTCACTA 
ACCGCACATA GCGGTACAAT GTCCAGTAAA ACACGCGAAC ACAAGAAAGC AACCTCAAAC 
GCCATTGAGA AGCCGCACGA ACGAGCCATC ACACCAACGT ACGTATACGA AACCGAAACG 
AATTTACCAC ATATACAAAC CGATAGCAAC AGTAGTGAGA GTTTAGTACA GACGCAAACC 
ACAATTGCTG CTATGACGCA CAGCACAACA CATGAATGTC ACGAAAATTA TGCGCGACTG 
AAGTCAGAGC TTAAAACGCA TACAAATGCT GCGACACAAC ATGCAAGCTT AACCGCAACA 
CAATATGACT ATTGTACGGA GGTGGCACCA TTACCACCGC CGCGCACACG CCGCAAGCGT 
CATAGATCAC TCATACCTAT ACCCATAAAT CCTAGTACAC CAACGCCAAC AGCAACAACA 
TCCCCATCTC CATCCTCGTT GCCCATTACA TATGAACATC AGATTTATGC ACAAATCATA 
CCGCCTGTTG CGTATGCACC CGCACAACCC ACATATTCCA ATCAGTCGAC ATTACGTAAA 
TGTTCATTGC AACGCATTAT GCGTAGACGT AGCGCTTTAC CCTTGCCCCT CACATTCCCA 
TGTGCGCGCA AACCATCGCA AACACAATCG ACCACCTCCT CATTCGCATT CCTACCCTAT 
TTACCAGCCG CGCCCGGTAA GCGGCCCTCA GACGCGAGTC ATATTTATCA TAATTATTTA 
TTGTATGCAA CGCCGGCGAA AGTTGAAGCA GAGCCGTTGA ATTTGACGCA ACCAGCCGAT 
GAGTTGGCGA CATTTTGGCA CGCCGATGCG CTACACCGAC CGGAGCGGCC GCAGCGTTTG 
CGCACAATGC GTAGTCATGT GTCGACGCAT CATCCGTTCG CATTGTGCAC ATGCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176664.1
CDS	894..8033
Translation	MEVATTNTHHHNHHHQHLGHQLHHPAHRLPMAKSTQHQQQQQQSNHCLTVSNDSAQSGGGGGGGSPRSPFQREVREWQRIYPDTGALLSGRLEADRWINGPLNSYGKISDSQNISQPNGTQHTQRKQLEVLQKRTANGAVQVIRAQTVQTSSSRSWSSSNSNSASTSASASASASPICMQANVVGARSLLHNHTNNNINNINSSKHFHSTLQQHLAEANTHWLQLSPPTIAATVDISEVSTDDDLDKAVPTIAETPYIVDDSEIDVGNGCGVDADINASATLCNYSISRSGSAGSGGGGSSKGHALLSSAASSSSTLHSQSSALLVPDTAPSLKLSNLMKSSSGGSSSSNNSTKLNTHKSAGGKVSLHAIVGSAGDNNTNSGSASRINNDLTESPKSTTTTAKSSNEALSGTKTWQQQQRRSNARHLEQATLYGQNSNSSKMRVGGADCDDCYAVVSADADESQLRYHQHHLQPQQLRKQQQQQQQSSVVLSETTPPTRRRDLESFRLYGDSTTAATTAGAAADDNAKADDLRLSTRQLRRQQQPFGQLSSSALIQRSHSISTDDLSNEWESNEAVAESNEWRRVSKLRRSFQSSSTANNSNTSAPAVQQPTSSIRRSYDLPVSAVSVSRIRAELESGRRLTTAMRNNHVDLAALESILHGPGKTTDTSSRNTLLTAESLKEIRGRLKKLSDESLYKDDFIAYQQPPSADEHIEVQQSVRRKLSTPRLQQLTPEESPEISTPTQHTSAFKVVGAQRKPTDNDSLTQRSTHLNQKHTSNSLESRQKQRDTNSTEWHSRRKSYGFEKMSPPDNKTMHRMDASTDSGLGRSGELGNWSPTEAAPPRATVIHFGEPAKAVTSAHYRAAKSPVRRTPDEDLLKRHSIAVDESQYVRDNLRKTSQVHLNGFYDPAEPAAKSTVQLSRYDSNAESNQLFQRNTQNAQKRVEFCKTEVHFAAESGRVNIVETDGKPPPTNNFRRRRRTTSGPLQSLTKTASTAGTSTAVSTTTASNVTHFGDDQHQQQRHRTIASSVVAYKATLVEPPTVLNDAQIMPATTNVTVSVQHAEGSRGSYASTTSGVDTTDNENDEIAAIRGILKNKPVKPKPYHLGENIDSADALWGVQLKPVAGGGSSAAREKEDSPTAVLNKSVAERVRIVEKRHEHNAPNGYSTKINLSLDEPAAAARDWPNAGAPMLMPNTDNQPIPNNTNRRASAQELILQDLREHQRMLDEGLKSTSLIIKTMRSANEFDEAMRRLSIASLESSTMPPIVVPTPMLRSNSYQETVRRYSNASLESTERELTTQTVVHTSSSAAALTAATSSRRLSFETQSAFVTPLSLPPAPLVAPRLKLLGSTEVSVSQQLSQLRRMYDIAATQQENDEDAADSADEEVKSYFCAHEQAEQSDSGGGTLESSSQEDERAIEYSSGSWSRMKAKRTIWKIEAEERKAAPAVLDKADLPKSNIMSIELHSPQTSLEKRDSIKRNQTPPVAKPRTVTGAVNATQHPQIHVATTVKQTADRFSAAEQQRQSLKIVKEARGARKLREHELSYFGVPHGQKHEENETKPKQQPNSNANTLSLSKRSLPSRQRLTVIGNEDTKSMRRHEANNTETTTVSGQTQTATKWQLLNDKPDLLRHSPIAVEPQLIRRTADVQRSERNSTSVERNKRMPSKELRLDDELNDAVEDDDDDNEHFYENITNEITPVFKVKSPQPYDRKMDMERDAFILNEMNENADLTMKALSDEAAHKDRRRRRSSLHRRDSKPLETIEEKLVTQKPNDTCIAVKMSRGTLASEAKRHARHTTTSPSPHDTTTRVRTSSQSSVESCPRARSRSLSSEREYENIRTPKPTRTNATATTKIQAKSVSKQQQRSSSRESQRSARLPYSSGDEVNPREEQRVRMKSKRSSNSTSGTTGRSGERQRSSKKDSHHDKHGHHTTTTSSRDVEQRRRAASSTTHASQREERGECVTVPQQQRTASSGRSSSSNKRREEPATTAGSSGRHSSSRNATEKARTSAGVRLLRSLRVGRTDDAKASSTEQRASGHHRSTSEREKAHEKEREKELQSKSRGHSTSKTQHHSSSEHHQHSLTAHSGTMSSKTREHKKATSNAIEKPHERAITPTYVYETETNLPHIQTDSNSSESLVQTQTTIAAMTHSTTHECHENYARLKSELKTHTNAATQHASLTATQYDYCTEVAPLPPPRTRRKRHRSLIPIPINPSTPTPTATTSPSPSSLPITYEHQIYAQIIPPVAYAPAQPTYSNQSTLRKCSLQRIMRRRSALPLPLTFPCARKPSQTQSTTSSFAFLPYLPAAPGKRPSDASHIYHNYLLYATPAKVEAEPLNLTQPADELATFWHADALHRPERPQRLRTMRSHVSTHHPFALCTCS

Target ORF information:

RefSeq Version	XM_011178362.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178362.1

ORF Insert Sequence:

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GGTCACTCAA CCAGCAAAAC CCAGCACCAC AGCAGTAGTG AGCACCACCA ACATTCACTA 
ACCGCACATA GCGGTACAAT GTCCAGTAAA ACACGCGAAC ACAAGAAAGC AACCTCAAAC 
GCCATTGAGA AGCCGCACGA ACGAGCCATC ACACCAACGT ACGTATACGA AACCGAAACG 
AATTTACCAC ATATACAAAC CGATAGCAAC AGTAGTGAGA GTTTAGTACA GACGCAAACC 
ACAATTGCTG CTATGACGCA CAGCACAACA CATGAATGTC ACGAAAATTA TGCGCGACTG 
AAGTCAGAGC TTAAAACGCA TACAAATGCT GCGACACAAC ATGCAAGCTT AACCGCAACA 
CAATATGACT ATTGTACGGA GGTGGCACCA TTACCACCGC CGCGCACACG CCGCAAGCGT 
CATAGATCAC TCATACCTAT ACCCATAAAT CCTAGTACAC CAACGCCAAC AGCAACAACA 
TCCCCATCTC CATCCTCGTT GCCCATTACA TATGAACATC AGATTTATGC ACAAATCATA 
CCGCCTGTTG CGTATGCACC CGCACAACCC ACATATTCCA ATCAGTCGAC ATTACGTAAA 
TGTTCATTGC AACGCATTAT GCGTAGACGT AGCGCTTTAC CCTTGCCCCT CACATTCCCA 
TGTGCGCGCA AACCATCGCA AACACAATCG ACCACCTCCT CATTCGCATT CCTACCCTAT 
TTACCAGCCG CGCCCGGTAA GCGGCCCTCA GACGCGAGTC ATATTTATCA TAATTATTTA 
TTGTATGCAA CGCCGGCGAA AGTTGAAGCA GAGCCGTTGA ATTTGACGCA ACCAGCCGAT 
GAGTTGGCGA CATTTTGGCA CGCCGATGCG CTACACCGAC CGGAGCGGCC GCAGCGTTTG 
CGCACAATGC GTAGTCATGT GTCGACGCAT CATCCGTTCG CATTGTGCAC ATGCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208490 ( XM_011178359.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176661.1 Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X1, mRNA.

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CloneID	OBa194180
Clone ID Related Accession (Same CDS sequence)	XM_011178360.1 , XM_011178362.1 , XM_011178359.1
Accession Version	XM_011178359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105208490 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863673.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GGTCACTCAA CCAGCAAAAC CCAGCACCAC AGCAGTAGTG AGCACCACCA ACATTCACTA 
ACCGCACATA GCGGTACAAT GTCCAGTAAA ACACGCGAAC ACAAGAAAGC AACCTCAAAC 
GCCATTGAGA AGCCGCACGA ACGAGCCATC ACACCAACGT ACGTATACGA AACCGAAACG 
AATTTACCAC ATATACAAAC CGATAGCAAC AGTAGTGAGA GTTTAGTACA GACGCAAACC 
ACAATTGCTG CTATGACGCA CAGCACAACA CATGAATGTC ACGAAAATTA TGCGCGACTG 
AAGTCAGAGC TTAAAACGCA TACAAATGCT GCGACACAAC ATGCAAGCTT AACCGCAACA 
CAATATGACT ATTGTACGGA GGTGGCACCA TTACCACCGC CGCGCACACG CCGCAAGCGT 
CATAGATCAC TCATACCTAT ACCCATAAAT CCTAGTACAC CAACGCCAAC AGCAACAACA 
TCCCCATCTC CATCCTCGTT GCCCATTACA TATGAACATC AGATTTATGC ACAAATCATA 
CCGCCTGTTG CGTATGCACC CGCACAACCC ACATATTCCA ATCAGTCGAC ATTACGTAAA 
TGTTCATTGC AACGCATTAT GCGTAGACGT AGCGCTTTAC CCTTGCCCCT CACATTCCCA 
TGTGCGCGCA AACCATCGCA AACACAATCG ACCACCTCCT CATTCGCATT CCTACCCTAT 
TTACCAGCCG CGCCCGGTAA GCGGCCCTCA GACGCGAGTC ATATTTATCA TAATTATTTA 
TTGTATGCAA CGCCGGCGAA AGTTGAAGCA GAGCCGTTGA ATTTGACGCA ACCAGCCGAT 
GAGTTGGCGA CATTTTGGCA CGCCGATGCG CTACACCGAC CGGAGCGGCC GCAGCGTTTG 
CGCACAATGC GTAGTCATGT GTCGACGCAT CATCCGTTCG CATTGTGCAC ATGCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176661.1
CDS	950..8089
Translation	MEVATTNTHHHNHHHQHLGHQLHHPAHRLPMAKSTQHQQQQQQSNHCLTVSNDSAQSGGGGGGGSPRSPFQREVREWQRIYPDTGALLSGRLEADRWINGPLNSYGKISDSQNISQPNGTQHTQRKQLEVLQKRTANGAVQVIRAQTVQTSSSRSWSSSNSNSASTSASASASASPICMQANVVGARSLLHNHTNNNINNINSSKHFHSTLQQHLAEANTHWLQLSPPTIAATVDISEVSTDDDLDKAVPTIAETPYIVDDSEIDVGNGCGVDADINASATLCNYSISRSGSAGSGGGGSSKGHALLSSAASSSSTLHSQSSALLVPDTAPSLKLSNLMKSSSGGSSSSNNSTKLNTHKSAGGKVSLHAIVGSAGDNNTNSGSASRINNDLTESPKSTTTTAKSSNEALSGTKTWQQQQRRSNARHLEQATLYGQNSNSSKMRVGGADCDDCYAVVSADADESQLRYHQHHLQPQQLRKQQQQQQQSSVVLSETTPPTRRRDLESFRLYGDSTTAATTAGAAADDNAKADDLRLSTRQLRRQQQPFGQLSSSALIQRSHSISTDDLSNEWESNEAVAESNEWRRVSKLRRSFQSSSTANNSNTSAPAVQQPTSSIRRSYDLPVSAVSVSRIRAELESGRRLTTAMRNNHVDLAALESILHGPGKTTDTSSRNTLLTAESLKEIRGRLKKLSDESLYKDDFIAYQQPPSADEHIEVQQSVRRKLSTPRLQQLTPEESPEISTPTQHTSAFKVVGAQRKPTDNDSLTQRSTHLNQKHTSNSLESRQKQRDTNSTEWHSRRKSYGFEKMSPPDNKTMHRMDASTDSGLGRSGELGNWSPTEAAPPRATVIHFGEPAKAVTSAHYRAAKSPVRRTPDEDLLKRHSIAVDESQYVRDNLRKTSQVHLNGFYDPAEPAAKSTVQLSRYDSNAESNQLFQRNTQNAQKRVEFCKTEVHFAAESGRVNIVETDGKPPPTNNFRRRRRTTSGPLQSLTKTASTAGTSTAVSTTTASNVTHFGDDQHQQQRHRTIASSVVAYKATLVEPPTVLNDAQIMPATTNVTVSVQHAEGSRGSYASTTSGVDTTDNENDEIAAIRGILKNKPVKPKPYHLGENIDSADALWGVQLKPVAGGGSSAAREKEDSPTAVLNKSVAERVRIVEKRHEHNAPNGYSTKINLSLDEPAAAARDWPNAGAPMLMPNTDNQPIPNNTNRRASAQELILQDLREHQRMLDEGLKSTSLIIKTMRSANEFDEAMRRLSIASLESSTMPPIVVPTPMLRSNSYQETVRRYSNASLESTERELTTQTVVHTSSSAAALTAATSSRRLSFETQSAFVTPLSLPPAPLVAPRLKLLGSTEVSVSQQLSQLRRMYDIAATQQENDEDAADSADEEVKSYFCAHEQAEQSDSGGGTLESSSQEDERAIEYSSGSWSRMKAKRTIWKIEAEERKAAPAVLDKADLPKSNIMSIELHSPQTSLEKRDSIKRNQTPPVAKPRTVTGAVNATQHPQIHVATTVKQTADRFSAAEQQRQSLKIVKEARGARKLREHELSYFGVPHGQKHEENETKPKQQPNSNANTLSLSKRSLPSRQRLTVIGNEDTKSMRRHEANNTETTTVSGQTQTATKWQLLNDKPDLLRHSPIAVEPQLIRRTADVQRSERNSTSVERNKRMPSKELRLDDELNDAVEDDDDDNEHFYENITNEITPVFKVKSPQPYDRKMDMERDAFILNEMNENADLTMKALSDEAAHKDRRRRRSSLHRRDSKPLETIEEKLVTQKPNDTCIAVKMSRGTLASEAKRHARHTTTSPSPHDTTTRVRTSSQSSVESCPRARSRSLSSEREYENIRTPKPTRTNATATTKIQAKSVSKQQQRSSSRESQRSARLPYSSGDEVNPREEQRVRMKSKRSSNSTSGTTGRSGERQRSSKKDSHHDKHGHHTTTTSSRDVEQRRRAASSTTHASQREERGECVTVPQQQRTASSGRSSSSNKRREEPATTAGSSGRHSSSRNATEKARTSAGVRLLRSLRVGRTDDAKASSTEQRASGHHRSTSEREKAHEKEREKELQSKSRGHSTSKTQHHSSSEHHQHSLTAHSGTMSSKTREHKKATSNAIEKPHERAITPTYVYETETNLPHIQTDSNSSESLVQTQTTIAAMTHSTTHECHENYARLKSELKTHTNAATQHASLTATQYDYCTEVAPLPPPRTRRKRHRSLIPIPINPSTPTPTATTSPSPSSLPITYEHQIYAQIIPPVAYAPAQPTYSNQSTLRKCSLQRIMRRRSALPLPLTFPCARKPSQTQSTTSSFAFLPYLPAAPGKRPSDASHIYHNYLLYATPAKVEAEPLNLTQPADELATFWHADALHRPERPQRLRTMRSHVSTHHPFALCTCS

Target ORF information:

RefSeq Version	XM_011178359.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178359.1

ORF Insert Sequence:

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GGTCACTCAA CCAGCAAAAC CCAGCACCAC AGCAGTAGTG AGCACCACCA ACATTCACTA 
ACCGCACATA GCGGTACAAT GTCCAGTAAA ACACGCGAAC ACAAGAAAGC AACCTCAAAC 
GCCATTGAGA AGCCGCACGA ACGAGCCATC ACACCAACGT ACGTATACGA AACCGAAACG 
AATTTACCAC ATATACAAAC CGATAGCAAC AGTAGTGAGA GTTTAGTACA GACGCAAACC 
ACAATTGCTG CTATGACGCA CAGCACAACA CATGAATGTC ACGAAAATTA TGCGCGACTG 
AAGTCAGAGC TTAAAACGCA TACAAATGCT GCGACACAAC ATGCAAGCTT AACCGCAACA 
CAATATGACT ATTGTACGGA GGTGGCACCA TTACCACCGC CGCGCACACG CCGCAAGCGT 
CATAGATCAC TCATACCTAT ACCCATAAAT CCTAGTACAC CAACGCCAAC AGCAACAACA 
TCCCCATCTC CATCCTCGTT GCCCATTACA TATGAACATC AGATTTATGC ACAAATCATA 
CCGCCTGTTG CGTATGCACC CGCACAACCC ACATATTCCA ATCAGTCGAC ATTACGTAAA 
TGTTCATTGC AACGCATTAT GCGTAGACGT AGCGCTTTAC CCTTGCCCCT CACATTCCCA 
TGTGCGCGCA AACCATCGCA AACACAATCG ACCACCTCCT CATTCGCATT CCTACCCTAT 
TTACCAGCCG CGCCCGGTAA GCGGCCCTCA GACGCGAGTC ATATTTATCA TAATTATTTA 
TTGTATGCAA CGCCGGCGAA AGTTGAAGCA GAGCCGTTGA ATTTGACGCA ACCAGCCGAT 
GAGTTGGCGA CATTTTGGCA CGCCGATGCG CTACACCGAC CGGAGCGGCC GCAGCGTTTG 
CGCACAATGC GTAGTCATGT GTCGACGCAT CATCCGTTCG CATTGTGCAC ATGCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208490 ( XM_011178363.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176665.1 Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X1, mRNA.

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CloneID	OBa194181
Clone ID Related Accession (Same CDS sequence)	XM_011178363.2 , XM_011178363.1 , XM_029037936.1 , XM_029037937.1
Accession Version	XM_011178363.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105208490 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863673.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011178363.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GGTCACTCAA CCAGCAAAAC CCAGCACCAC AGCAGTAGTG AGCACCACCA ACATTCACTA 
ACCGCACATA GCGGTACAAT GTCCAGTAAA ACACGCGAAC ACAAGAAAGC AACCTCAAAC 
GCCATTGAGA AGCCGCACGA ACGAGCCATC ACACCAACAC ACAGTAAAAG CTCGCAAAAC 
ATAAATGGAC GACATACTAC TACTGGACAA CATGATGGCC ACCACCGCAA ACATAACGAT 
CATAAACGAG CACACACACG ACCACAACAA GAAGAGAGCA GAACAACGAA GACAACGACG 
ACGCAGCGAA AGCGTAGTGA ACATAGCGCA AGGGAGCGCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176665.1
CDS	948..7472
Translation	MEVATTNTHHHNHHHQHLGHQLHHPAHRLPMAKSTQHQQQQQQSNHCLTVSNDSAQSGGGGGGGSPRSPFQREVREWQRIYPDTGALLSGRLEADRWINGPLNSYGKISDSQNISQPNGTQHTQRKQLEVLQKRTANGAVQVIRAQTVQTSSSRSWSSSNSNSASTSASASASASPICMQANVVGARSLLHNHTNNNINNINSSKHFHSTLQQHLAEANTHWLQLSPPTIAATVDISEVSTDDDLDKAVPTIAETPYIVDDSEIDVGNGCGVDADINASATLCNYSISRSGSAGSGGGGSSKGHALLSSAASSSSTLHSQSSALLVPDTAPSLKLSNLMKSSSGGSSSSNNSTKLNTHKSAGGKVSLHAIVGSAGDNNTNSGSASRINNDLTESPKSTTTTAKSSNEALSGTKTWQQQQRRSNARHLEQATLYGQNSNSSKMRVGGADCDDCYAVVSADADESQLRYHQHHLQPQQLRKQQQQQQQSSVVLSETTPPTRRRDLESFRLYGDSTTAATTAGAAADDNAKADDLRLSTRQLRRQQQPFGQLSSSALIQRSHSISTDDLSNEWESNEAVAESNEWRRVSKLRRSFQSSSTANNSNTSAPAVQQPTSSIRRSYDLPVSAVSVSRIRAELESGRRLTTAMRNNHVDLAALESILHGPGKTTDTSSRNTLLTAESLKEIRGRLKKLSDESLYKDDFIAYQQPPSADEHIEVQQSVRRKLSTPRLQQLTPEESPEISTPTQHTSAFKVVGAQRKPTDNDSLTQRSTHLNQKHTSNSLESRQKQRDTNSTEWHSRRKSYGFEKMSPPDNKTMHRMDASTDSGLGRSGELGNWSPTEAAPPRATVIHFGEPAKAVTSAHYRAAKSPVRRTPDEDLLKRHSIAVDESQYVRDNLRKTSQVHLNGFYDPAEPAAKSTVQLSRYDSNAESNQLFQRNTQNAQKRVEFCKTEVHFAAESGRVNIVETDGKPPPTNNFRRRRRTTSGPLQSLTKTASTAGTSTAVSTTTASNVTHFGDDQHQQQRHRTIASSVVAYKATLVEPPTVLNDAQIMPATTNVTVSVQHAEGSRGSYASTTSGVDTTDNENDEIAAIRGILKNKPVKPKPYHLGENIDSADALWGVQLKPVAGGGSSAAREKEDSPTAVLNKSVAERVRIVEKRHEHNAPNGYSTKINLSLDEPAAAARDWPNAGAPMLMPNTDNQPIPNNTNRRASAQELILQDLREHQRMLDEGLKSTSLIIKTMRSANEFDEAMRRLSIASLESSTMPPIVVPTPMLRSNSYQETVRRYSNASLESTERELTTQTVVHTSSSAAALTAATSSRRLSFETQSAFVTPLSLPPAPLVAPRLKLLGSTEVSVSQQLSQLRRMYDIAATQQENDEDAADSADEEVKSYFCAHEQAEQSDSGGGTLESSSQEDERAIEYSSGSWSRMKAKRTIWKIEAEERKAAPAVLDKADLPKSNIMSIELHSPQTSLEKRDSIKRNQTPPVAKPRTVTGAVNATQHPQIHVATTVKQTADRFSAAEQQRQSLKIVKEARGARKLREHELSYFGVPHGQKHEENETKPKQQPNSNANTLSLSKRSLPSRQRLTVIGNEDTKSMRRHEANNTETTTVSGQTQTATKWQLLNDKPDLLRHSPIAVEPQLIRRTADVQRSERNSTSVERNKRMPSKELRLDDELNDAVEDDDDDNEHFYENITNEITPVFKVKSPQPYDRKMDMERDAFILNEMNENADLTMKALSDEAAHKDRRRRRSSLHRRDSKPLETIEEKLVTQKPNDTCIAVKMSRGTLASEAKRHARHTTTSPSPHDTTTRVRTSSQSSVESCPRARSRSLSSEREYENIRTPKPTRTNATATTKIQAKSVSKQQQRSSSRESQRSARLPYSSGDEVNPREEQRVRMKSKRSSNSTSGTTGRSGERQRSSKKDSHHDKHGHHTTTTSSRDVEQRRRAASSTTHASQREERGECVTVPQQQRTASSGRSSSSNKRREEPATTAGSSGRHSSSRNATEKARTSAGVRLLRSLRVGRTDDAKASSTEQRASGHHRSTSEREKAHEKEREKELQSKSRGHSTSKTQHHSSSEHHQHSLTAHSGTMSSKTREHKKATSNAIEKPHERAITPTHSKSSQNINGRHTTTGQHDGHHRKHNDHKRAHTRPQQEESRTTKTTTTQRKRSEHSARERK

Target ORF information:

RefSeq Version	XM_011178363.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178363.2

ORF Insert Sequence:

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GGTCACTCAA CCAGCAAAAC CCAGCACCAC AGCAGTAGTG AGCACCACCA ACATTCACTA 
ACCGCACATA GCGGTACAAT GTCCAGTAAA ACACGCGAAC ACAAGAAAGC AACCTCAAAC 
GCCATTGAGA AGCCGCACGA ACGAGCCATC ACACCAACAC ACAGTAAAAG CTCGCAAAAC 
ATAAATGGAC GACATACTAC TACTGGACAA CATGATGGCC ACCACCGCAA ACATAACGAT 
CATAAACGAG CACACACACG ACCACAACAA GAAGAGAGCA GAACAACGAA GACAACGACG 
ACGCAGCGAA AGCGTAGTGA ACATAGCGCA AGGGAGCGCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208490 ( XM_011178363.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176665.1 Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X4, mRNA.

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CloneID	OBa194181
Clone ID Related Accession (Same CDS sequence)	XM_011178363.2 , XM_011178363.1 , XM_029037936.1 , XM_029037937.1
Accession Version	XM_011178363.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105208490 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863673.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GGTCACTCAA CCAGCAAAAC CCAGCACCAC AGCAGTAGTG AGCACCACCA ACATTCACTA 
ACCGCACATA GCGGTACAAT GTCCAGTAAA ACACGCGAAC ACAAGAAAGC AACCTCAAAC 
GCCATTGAGA AGCCGCACGA ACGAGCCATC ACACCAACAC ACAGTAAAAG CTCGCAAAAC 
ATAAATGGAC GACATACTAC TACTGGACAA CATGATGGCC ACCACCGCAA ACATAACGAT 
CATAAACGAG CACACACACG ACCACAACAA GAAGAGAGCA GAACAACGAA GACAACGACG 
ACGCAGCGAA AGCGTAGTGA ACATAGCGCA AGGGAGCGCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176665.1
CDS	950..7474
Translation	MEVATTNTHHHNHHHQHLGHQLHHPAHRLPMAKSTQHQQQQQQSNHCLTVSNDSAQSGGGGGGGSPRSPFQREVREWQRIYPDTGALLSGRLEADRWINGPLNSYGKISDSQNISQPNGTQHTQRKQLEVLQKRTANGAVQVIRAQTVQTSSSRSWSSSNSNSASTSASASASASPICMQANVVGARSLLHNHTNNNINNINSSKHFHSTLQQHLAEANTHWLQLSPPTIAATVDISEVSTDDDLDKAVPTIAETPYIVDDSEIDVGNGCGVDADINASATLCNYSISRSGSAGSGGGGSSKGHALLSSAASSSSTLHSQSSALLVPDTAPSLKLSNLMKSSSGGSSSSNNSTKLNTHKSAGGKVSLHAIVGSAGDNNTNSGSASRINNDLTESPKSTTTTAKSSNEALSGTKTWQQQQRRSNARHLEQATLYGQNSNSSKMRVGGADCDDCYAVVSADADESQLRYHQHHLQPQQLRKQQQQQQQSSVVLSETTPPTRRRDLESFRLYGDSTTAATTAGAAADDNAKADDLRLSTRQLRRQQQPFGQLSSSALIQRSHSISTDDLSNEWESNEAVAESNEWRRVSKLRRSFQSSSTANNSNTSAPAVQQPTSSIRRSYDLPVSAVSVSRIRAELESGRRLTTAMRNNHVDLAALESILHGPGKTTDTSSRNTLLTAESLKEIRGRLKKLSDESLYKDDFIAYQQPPSADEHIEVQQSVRRKLSTPRLQQLTPEESPEISTPTQHTSAFKVVGAQRKPTDNDSLTQRSTHLNQKHTSNSLESRQKQRDTNSTEWHSRRKSYGFEKMSPPDNKTMHRMDASTDSGLGRSGELGNWSPTEAAPPRATVIHFGEPAKAVTSAHYRAAKSPVRRTPDEDLLKRHSIAVDESQYVRDNLRKTSQVHLNGFYDPAEPAAKSTVQLSRYDSNAESNQLFQRNTQNAQKRVEFCKTEVHFAAESGRVNIVETDGKPPPTNNFRRRRRTTSGPLQSLTKTASTAGTSTAVSTTTASNVTHFGDDQHQQQRHRTIASSVVAYKATLVEPPTVLNDAQIMPATTNVTVSVQHAEGSRGSYASTTSGVDTTDNENDEIAAIRGILKNKPVKPKPYHLGENIDSADALWGVQLKPVAGGGSSAAREKEDSPTAVLNKSVAERVRIVEKRHEHNAPNGYSTKINLSLDEPAAAARDWPNAGAPMLMPNTDNQPIPNNTNRRASAQELILQDLREHQRMLDEGLKSTSLIIKTMRSANEFDEAMRRLSIASLESSTMPPIVVPTPMLRSNSYQETVRRYSNASLESTERELTTQTVVHTSSSAAALTAATSSRRLSFETQSAFVTPLSLPPAPLVAPRLKLLGSTEVSVSQQLSQLRRMYDIAATQQENDEDAADSADEEVKSYFCAHEQAEQSDSGGGTLESSSQEDERAIEYSSGSWSRMKAKRTIWKIEAEERKAAPAVLDKADLPKSNIMSIELHSPQTSLEKRDSIKRNQTPPVAKPRTVTGAVNATQHPQIHVATTVKQTADRFSAAEQQRQSLKIVKEARGARKLREHELSYFGVPHGQKHEENETKPKQQPNSNANTLSLSKRSLPSRQRLTVIGNEDTKSMRRHEANNTETTTVSGQTQTATKWQLLNDKPDLLRHSPIAVEPQLIRRTADVQRSERNSTSVERNKRMPSKELRLDDELNDAVEDDDDDNEHFYENITNEITPVFKVKSPQPYDRKMDMERDAFILNEMNENADLTMKALSDEAAHKDRRRRRSSLHRRDSKPLETIEEKLVTQKPNDTCIAVKMSRGTLASEAKRHARHTTTSPSPHDTTTRVRTSSQSSVESCPRARSRSLSSEREYENIRTPKPTRTNATATTKIQAKSVSKQQQRSSSRESQRSARLPYSSGDEVNPREEQRVRMKSKRSSNSTSGTTGRSGERQRSSKKDSHHDKHGHHTTTTSSRDVEQRRRAASSTTHASQREERGECVTVPQQQRTASSGRSSSSNKRREEPATTAGSSGRHSSSRNATEKARTSAGVRLLRSLRVGRTDDAKASSTEQRASGHHRSTSEREKAHEKEREKELQSKSRGHSTSKTQHHSSSEHHQHSLTAHSGTMSSKTREHKKATSNAIEKPHERAITPTHSKSSQNINGRHTTTGQHDGHHRKHNDHKRAHTRPQQEESRTTKTTTTQRKRSEHSARERK

Target ORF information:

RefSeq Version	XM_011178363.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178363.1

ORF Insert Sequence:

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GGTCACTCAA CCAGCAAAAC CCAGCACCAC AGCAGTAGTG AGCACCACCA ACATTCACTA 
ACCGCACATA GCGGTACAAT GTCCAGTAAA ACACGCGAAC ACAAGAAAGC AACCTCAAAC 
GCCATTGAGA AGCCGCACGA ACGAGCCATC ACACCAACAC ACAGTAAAAG CTCGCAAAAC 
ATAAATGGAC GACATACTAC TACTGGACAA CATGATGGCC ACCACCGCAA ACATAACGAT 
CATAAACGAG CACACACACG ACCACAACAA GAAGAGAGCA GAACAACGAA GACAACGACG 
ACGCAGCGAA AGCGTAGTGA ACATAGCGCA AGGGAGCGCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208490 ( XM_029037936.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028893769.1 Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X2, mRNA.

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CloneID	OBa194181
Clone ID Related Accession (Same CDS sequence)	XM_011178363.2 , XM_011178363.1 , XM_029037936.1 , XM_029037937.1
Accession Version	XM_029037936.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105208490 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863673.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GGTCACTCAA CCAGCAAAAC CCAGCACCAC AGCAGTAGTG AGCACCACCA ACATTCACTA 
ACCGCACATA GCGGTACAAT GTCCAGTAAA ACACGCGAAC ACAAGAAAGC AACCTCAAAC 
GCCATTGAGA AGCCGCACGA ACGAGCCATC ACACCAACAC ACAGTAAAAG CTCGCAAAAC 
ATAAATGGAC GACATACTAC TACTGGACAA CATGATGGCC ACCACCGCAA ACATAACGAT 
CATAAACGAG CACACACACG ACCACAACAA GAAGAGAGCA GAACAACGAA GACAACGACG 
ACGCAGCGAA AGCGTAGTGA ACATAGCGCA AGGGAGCGCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028893769.1
CDS	894..7418
Translation	MEVATTNTHHHNHHHQHLGHQLHHPAHRLPMAKSTQHQQQQQQSNHCLTVSNDSAQSGGGGGGGSPRSPFQREVREWQRIYPDTGALLSGRLEADRWINGPLNSYGKISDSQNISQPNGTQHTQRKQLEVLQKRTANGAVQVIRAQTVQTSSSRSWSSSNSNSASTSASASASASPICMQANVVGARSLLHNHTNNNINNINSSKHFHSTLQQHLAEANTHWLQLSPPTIAATVDISEVSTDDDLDKAVPTIAETPYIVDDSEIDVGNGCGVDADINASATLCNYSISRSGSAGSGGGGSSKGHALLSSAASSSSTLHSQSSALLVPDTAPSLKLSNLMKSSSGGSSSSNNSTKLNTHKSAGGKVSLHAIVGSAGDNNTNSGSASRINNDLTESPKSTTTTAKSSNEALSGTKTWQQQQRRSNARHLEQATLYGQNSNSSKMRVGGADCDDCYAVVSADADESQLRYHQHHLQPQQLRKQQQQQQQSSVVLSETTPPTRRRDLESFRLYGDSTTAATTAGAAADDNAKADDLRLSTRQLRRQQQPFGQLSSSALIQRSHSISTDDLSNEWESNEAVAESNEWRRVSKLRRSFQSSSTANNSNTSAPAVQQPTSSIRRSYDLPVSAVSVSRIRAELESGRRLTTAMRNNHVDLAALESILHGPGKTTDTSSRNTLLTAESLKEIRGRLKKLSDESLYKDDFIAYQQPPSADEHIEVQQSVRRKLSTPRLQQLTPEESPEISTPTQHTSAFKVVGAQRKPTDNDSLTQRSTHLNQKHTSNSLESRQKQRDTNSTEWHSRRKSYGFEKMSPPDNKTMHRMDASTDSGLGRSGELGNWSPTEAAPPRATVIHFGEPAKAVTSAHYRAAKSPVRRTPDEDLLKRHSIAVDESQYVRDNLRKTSQVHLNGFYDPAEPAAKSTVQLSRYDSNAESNQLFQRNTQNAQKRVEFCKTEVHFAAESGRVNIVETDGKPPPTNNFRRRRRTTSGPLQSLTKTASTAGTSTAVSTTTASNVTHFGDDQHQQQRHRTIASSVVAYKATLVEPPTVLNDAQIMPATTNVTVSVQHAEGSRGSYASTTSGVDTTDNENDEIAAIRGILKNKPVKPKPYHLGENIDSADALWGVQLKPVAGGGSSAAREKEDSPTAVLNKSVAERVRIVEKRHEHNAPNGYSTKINLSLDEPAAAARDWPNAGAPMLMPNTDNQPIPNNTNRRASAQELILQDLREHQRMLDEGLKSTSLIIKTMRSANEFDEAMRRLSIASLESSTMPPIVVPTPMLRSNSYQETVRRYSNASLESTERELTTQTVVHTSSSAAALTAATSSRRLSFETQSAFVTPLSLPPAPLVAPRLKLLGSTEVSVSQQLSQLRRMYDIAATQQENDEDAADSADEEVKSYFCAHEQAEQSDSGGGTLESSSQEDERAIEYSSGSWSRMKAKRTIWKIEAEERKAAPAVLDKADLPKSNIMSIELHSPQTSLEKRDSIKRNQTPPVAKPRTVTGAVNATQHPQIHVATTVKQTADRFSAAEQQRQSLKIVKEARGARKLREHELSYFGVPHGQKHEENETKPKQQPNSNANTLSLSKRSLPSRQRLTVIGNEDTKSMRRHEANNTETTTVSGQTQTATKWQLLNDKPDLLRHSPIAVEPQLIRRTADVQRSERNSTSVERNKRMPSKELRLDDELNDAVEDDDDDNEHFYENITNEITPVFKVKSPQPYDRKMDMERDAFILNEMNENADLTMKALSDEAAHKDRRRRRSSLHRRDSKPLETIEEKLVTQKPNDTCIAVKMSRGTLASEAKRHARHTTTSPSPHDTTTRVRTSSQSSVESCPRARSRSLSSEREYENIRTPKPTRTNATATTKIQAKSVSKQQQRSSSRESQRSARLPYSSGDEVNPREEQRVRMKSKRSSNSTSGTTGRSGERQRSSKKDSHHDKHGHHTTTTSSRDVEQRRRAASSTTHASQREERGECVTVPQQQRTASSGRSSSSNKRREEPATTAGSSGRHSSSRNATEKARTSAGVRLLRSLRVGRTDDAKASSTEQRASGHHRSTSEREKAHEKEREKELQSKSRGHSTSKTQHHSSSEHHQHSLTAHSGTMSSKTREHKKATSNAIEKPHERAITPTHSKSSQNINGRHTTTGQHDGHHRKHNDHKRAHTRPQQEESRTTKTTTTQRKRSEHSARERK

Target ORF information:

RefSeq Version	XM_029037936.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029037936.1

ORF Insert Sequence:

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GGTCACTCAA CCAGCAAAAC CCAGCACCAC AGCAGTAGTG AGCACCACCA ACATTCACTA 
ACCGCACATA GCGGTACAAT GTCCAGTAAA ACACGCGAAC ACAAGAAAGC AACCTCAAAC 
GCCATTGAGA AGCCGCACGA ACGAGCCATC ACACCAACAC ACAGTAAAAG CTCGCAAAAC 
ATAAATGGAC GACATACTAC TACTGGACAA CATGATGGCC ACCACCGCAA ACATAACGAT 
CATAAACGAG CACACACACG ACCACAACAA GAAGAGAGCA GAACAACGAA GACAACGACG 
ACGCAGCGAA AGCGTAGTGA ACATAGCGCA AGGGAGCGCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208490 ( XM_029037937.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028893770.1 Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X3, mRNA.

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CloneID	OBa194181
Clone ID Related Accession (Same CDS sequence)	XM_011178363.2 , XM_011178363.1 , XM_029037936.1 , XM_029037937.1
Accession Version	XM_029037937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105208490 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863673.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GGTCACTCAA CCAGCAAAAC CCAGCACCAC AGCAGTAGTG AGCACCACCA ACATTCACTA 
ACCGCACATA GCGGTACAAT GTCCAGTAAA ACACGCGAAC ACAAGAAAGC AACCTCAAAC 
GCCATTGAGA AGCCGCACGA ACGAGCCATC ACACCAACAC ACAGTAAAAG CTCGCAAAAC 
ATAAATGGAC GACATACTAC TACTGGACAA CATGATGGCC ACCACCGCAA ACATAACGAT 
CATAAACGAG CACACACACG ACCACAACAA GAAGAGAGCA GAACAACGAA GACAACGACG 
ACGCAGCGAA AGCGTAGTGA ACATAGCGCA AGGGAGCGCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028893770.1
CDS	1001..7525
Translation	MEVATTNTHHHNHHHQHLGHQLHHPAHRLPMAKSTQHQQQQQQSNHCLTVSNDSAQSGGGGGGGSPRSPFQREVREWQRIYPDTGALLSGRLEADRWINGPLNSYGKISDSQNISQPNGTQHTQRKQLEVLQKRTANGAVQVIRAQTVQTSSSRSWSSSNSNSASTSASASASASPICMQANVVGARSLLHNHTNNNINNINSSKHFHSTLQQHLAEANTHWLQLSPPTIAATVDISEVSTDDDLDKAVPTIAETPYIVDDSEIDVGNGCGVDADINASATLCNYSISRSGSAGSGGGGSSKGHALLSSAASSSSTLHSQSSALLVPDTAPSLKLSNLMKSSSGGSSSSNNSTKLNTHKSAGGKVSLHAIVGSAGDNNTNSGSASRINNDLTESPKSTTTTAKSSNEALSGTKTWQQQQRRSNARHLEQATLYGQNSNSSKMRVGGADCDDCYAVVSADADESQLRYHQHHLQPQQLRKQQQQQQQSSVVLSETTPPTRRRDLESFRLYGDSTTAATTAGAAADDNAKADDLRLSTRQLRRQQQPFGQLSSSALIQRSHSISTDDLSNEWESNEAVAESNEWRRVSKLRRSFQSSSTANNSNTSAPAVQQPTSSIRRSYDLPVSAVSVSRIRAELESGRRLTTAMRNNHVDLAALESILHGPGKTTDTSSRNTLLTAESLKEIRGRLKKLSDESLYKDDFIAYQQPPSADEHIEVQQSVRRKLSTPRLQQLTPEESPEISTPTQHTSAFKVVGAQRKPTDNDSLTQRSTHLNQKHTSNSLESRQKQRDTNSTEWHSRRKSYGFEKMSPPDNKTMHRMDASTDSGLGRSGELGNWSPTEAAPPRATVIHFGEPAKAVTSAHYRAAKSPVRRTPDEDLLKRHSIAVDESQYVRDNLRKTSQVHLNGFYDPAEPAAKSTVQLSRYDSNAESNQLFQRNTQNAQKRVEFCKTEVHFAAESGRVNIVETDGKPPPTNNFRRRRRTTSGPLQSLTKTASTAGTSTAVSTTTASNVTHFGDDQHQQQRHRTIASSVVAYKATLVEPPTVLNDAQIMPATTNVTVSVQHAEGSRGSYASTTSGVDTTDNENDEIAAIRGILKNKPVKPKPYHLGENIDSADALWGVQLKPVAGGGSSAAREKEDSPTAVLNKSVAERVRIVEKRHEHNAPNGYSTKINLSLDEPAAAARDWPNAGAPMLMPNTDNQPIPNNTNRRASAQELILQDLREHQRMLDEGLKSTSLIIKTMRSANEFDEAMRRLSIASLESSTMPPIVVPTPMLRSNSYQETVRRYSNASLESTERELTTQTVVHTSSSAAALTAATSSRRLSFETQSAFVTPLSLPPAPLVAPRLKLLGSTEVSVSQQLSQLRRMYDIAATQQENDEDAADSADEEVKSYFCAHEQAEQSDSGGGTLESSSQEDERAIEYSSGSWSRMKAKRTIWKIEAEERKAAPAVLDKADLPKSNIMSIELHSPQTSLEKRDSIKRNQTPPVAKPRTVTGAVNATQHPQIHVATTVKQTADRFSAAEQQRQSLKIVKEARGARKLREHELSYFGVPHGQKHEENETKPKQQPNSNANTLSLSKRSLPSRQRLTVIGNEDTKSMRRHEANNTETTTVSGQTQTATKWQLLNDKPDLLRHSPIAVEPQLIRRTADVQRSERNSTSVERNKRMPSKELRLDDELNDAVEDDDDDNEHFYENITNEITPVFKVKSPQPYDRKMDMERDAFILNEMNENADLTMKALSDEAAHKDRRRRRSSLHRRDSKPLETIEEKLVTQKPNDTCIAVKMSRGTLASEAKRHARHTTTSPSPHDTTTRVRTSSQSSVESCPRARSRSLSSEREYENIRTPKPTRTNATATTKIQAKSVSKQQQRSSSRESQRSARLPYSSGDEVNPREEQRVRMKSKRSSNSTSGTTGRSGERQRSSKKDSHHDKHGHHTTTTSSRDVEQRRRAASSTTHASQREERGECVTVPQQQRTASSGRSSSSNKRREEPATTAGSSGRHSSSRNATEKARTSAGVRLLRSLRVGRTDDAKASSTEQRASGHHRSTSEREKAHEKEREKELQSKSRGHSTSKTQHHSSSEHHQHSLTAHSGTMSSKTREHKKATSNAIEKPHERAITPTHSKSSQNINGRHTTTGQHDGHHRKHNDHKRAHTRPQQEESRTTKTTTTQRKRSEHSARERK

Target ORF information:

RefSeq Version	XM_029037937.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029037937.1

ORF Insert Sequence:

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GGTCACTCAA CCAGCAAAAC CCAGCACCAC AGCAGTAGTG AGCACCACCA ACATTCACTA 
ACCGCACATA GCGGTACAAT GTCCAGTAAA ACACGCGAAC ACAAGAAAGC AACCTCAAAC 
GCCATTGAGA AGCCGCACGA ACGAGCCATC ACACCAACAC ACAGTAAAAG CTCGCAAAAC 
ATAAATGGAC GACATACTAC TACTGGACAA CATGATGGCC ACCACCGCAA ACATAACGAT 
CATAAACGAG CACACACACG ACCACAACAA GAAGAGAGCA GAACAACGAA GACAACGACG 
ACGCAGCGAA AGCGTAGTGA ACATAGCGCA AGGGAGCGCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208490 ( XM_011178365.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176667.1 Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X6, mRNA.

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CloneID	OBa194183
Clone ID Related Accession (Same CDS sequence)	XM_011178365.1
Accession Version	XM_011178365.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105208490 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863673.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGA TCTACCTAAA TCGAACATTA TGAGCATAGA ACTACATTCA 
CCACAAACTT CGTTGGAGAA ACGTGATTCC ATCAAACGCA ACCAAACACC GCCCGTGGCC 
AAACCGAGAA CTGTGACTGG TGCAGTCAAC GCCACTCAGC ATCCACAGAT ACATGTGGCC 
ACCACAGTCA AGCAAACAGC TGATCGTTTT TCAGCAGCCG AACAGCAACG CCAGTCACTG 
AAAATTGTCA AGGAAGCGCG TGGTGCACGT AAACTGCGTG AACACGAACT CTCATACTTT 
GGCGTACCAC ACGGACAGAA ACACGAAGAG AACGAAACAA AACCGAAGCA ACAGCCGAAT 
AGCAACGCTA ATACGTTGAG TTTATCGAAG CGTTCGCTAC CGTCCCGTCA ACGTTTAACT 
GTTATTGGTA ACGAGGATAC GAAATCGATG CGTCGACATG AAGCAAACAA CACTGAAACA 
ACAACCGTAA GCGGACAAAC ACAAACGGCC ACTAAATGGC AATTGTTGAA TGACAAACCC 
GATCTACTAC GACACAGTCC GATTGCTGTG GAACCACAAT TGATACGACG TACAGCAGAT 
GTGCAGCGTA GTGAACGTAA TAGCACATCC GTGGAACGTA ACAAACGTAT GCCCTCAAAA 
GAGTTGAGGT TAGATGATGA GCTCAATGAT GCTGTTGAAG ATGATGATGA CGACAACGAG 
CATTTTTATG AAAATATAAC AAATGAAATT ACGCCTGTCT TCAAAGTGAA GTCACCGCAA 
CCGTATGATC GTAAAATGGA TATGGAACGC GACGCATTCA TATTGAACGA GATGAATGAG 
AATGCCGATC TGACTATGAA GGCGCTCTCC GATGAGGCCG CCCATAAAGA TCGGCGACGA 
CGACGTTCAT CGCTCCATAG ACGCGACTCC AAACCACTAG AAACGATCGA AGAGAAATTG 
GTCACCCAAA AACCCAACGA CACATGCATT GCGGTGAAAA TGTCACGTGG CACGCTAGCC 
TCCGAAGCCA AACGCCACGC ACGTCACACA ACCACTTCAC CCAGCCCACA CGATACAACC 
ACACGAGTGC GTACTTCATC ACAATCGTCG GTAGAAAGTT GTCCGCGTGC ACGTTCGCGT 
TCACTCTCCA GCGAACGTGA GTACGAAAAC ATACGTACAC CGAAACCGAC GCGCACCAAC 
GCAACAGCCA CAACGAAAAT ACAAGCCAAA AGCGTTAGCA AACAACAGCA GCGCTCCAGT 
TCACGTGAAT CACAGCGTTC AGCGCGTTTG CCATATTCAT CGGGCGATGA GGTAAACCCA 
CGCGAGGAAC AACGTGTACG CATGAAGAGT AAACGTAGCT CGAACTCTAC GAGTGGCACA 
ACGGGTCGTA GTGGTGAACG ACAACGTAGC AGTAAGAAGG ATAGCCATCA CGACAAACAT 
GGACATCACA CGACAACAAC AAGCAGTCGA GATGTTGAGC AGCGACGGCG CGCGGCCAGC 
AGCACTACGC ATGCCTCTCA GCGAGAGGAA CGCGGTGAGT GCGTGACTGT GCCGCAACAA 
CAGCGCACCG CAAGTAGTGG ACGCAGCAGT AGCAGCAACA AGCGACGTGA AGAACCTGCC 
ACCACCGCTG GCAGTAGCGG ACGTCACTCC AGCTCACGCA ATGCCACGGA GAAGGCACGC 
ACTAGCGCTG GTGTGCGTCT ATTGCGCTCG CTACGCGTCG GACGTACGGA TGATGCCAAA 
GCGTCCAGCA CGGAGCAACG TGCGTCCGGA CATCATCGTT CCACCAGCGA GCGCGAGAAG 
GCACATGAGA AAGAGCGCGA AAAGGAGTTG CAGTCCAAAT CGCGAGGTCA CTCAACCAGC 
AAAACCCAGC ACCACAGCAG TAGTGAGCAC CACCAACATT CACTAACCGC ACATAGCGGT 
ACAATGTCCA GTAAAACACG CGAACACAAG AAAGCAACCT CAAACGCCAT TGAGAAGCCG 
CACGAACGAG CCATCACACC AACGTACGTA TACGAAACCG AAACGAATTT ACCACATATA 
CAAACCGATA GCAACAGTAG TGAGAGTTTA GTACAGACGC AAACCACAAT TGCTGCTATG 
ACGCACAGCA CAACACATGA ATGTCACGAA AATTATGCGC GACTGAAGTC AGAGCTTAAA 
ACGCATACAA ATGCTGCGAC ACAACATGCA AGCTTAACCG CAACACAATA TGACTATTGT 
ACGGAGGTGG CACCATTACC ACCGCCGCGC ACACGCCGCA AGCGTCATAG ATCACTCATA 
CCTATACCCA TAAATCCTAG TACACCAACG CCAACAGCAA CAACATCCCC ATCTCCATCC 
TCGTTGCCCA TTACATATGA ACATCAGATT TATGCACAAA TCATACCGCC TGTTGCGTAT 
GCACCCGCAC AACCCACATA TTCCAATCAG TCGACATTAC GTAAATGTTC ATTGCAACGC 
ATTATGCGTA GACGTAGCGC TTTACCCTTG CCCCTCACAT TCCCATGTGC GCGCAAACCA 
TCGCAAACAC AATCGACCAC CTCCTCATTC GCATTCCTAC CCTATTTACC AGCCGCGCCC 
GGTAAGCGGC CCTCAGACGC GAGTCATATT TATCATAATT ATTTATTGTA TGCAACGCCG 
GCGAAAGTTG AAGCAGAGCC GTTGAATTTG ACGCAACCAG CCGATGAGTT GGCGACATTT 
TGGCACGCCG ATGCGCTACA CCGACCGGAG CGGCCGCAGC GTTTGCGCAC AATGCGTAGT 
CATGTGTCGA CGCATCATCC GTTCGCATTG TGCACATGCT CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176667.1
CDS	950..7294
Translation	MEVATTNTHHHNHHHQHLGHQLHHPAHRLPMAKSTQHQQQQQQSNHCLTVSNDSAQSGGGGGGGSPRSPFQREVREWQRIYPDTGALLSGRLEADRWINGPLNSYGKISDSQNISQPNGTQHTQRKQLEVLQKRTANGAVQVIRAQTVQTSSSRSWSSSNSNSASTSASASASASPICMQANVVGARSLLHNHTNNNINNINSSKHFHSTLQQHLAEANTHWLQLSPPTIAATVDISEVSTDDDLDKAVPTIAETPYIVDDSEIDVGNGCGVDADINASATLCNYSISRSGSAGSGGGGSSKGHALLSSAASSSSTLHSQSSALLVPDTAPSLKLSNLMKSSSGGSSSSNNSTKLNTHKSAGGKVSLHAIVGSAGDNNTNSGSASRINNDLTESPKSTTTTAKSSNEALSGTKTWQQQQRRSNARHLEQATLYGQNSNSSKMRVGGADCDDCYAVVSADADESQLRYHQHHLQPQQLRKQQQQQQQSSVVLSETTPPTRRRDLESFRLYGDSTTAATTAGAAADDNAKADDLRLSTRQLRRQQQPFGQLSSSALIQRSHSISTDDLSNEWESNEAVAESNEWRRVSKLRRSFQSSSTANNSNTSAPAVQQPTSSIRRSYDLPVSAVSVSRIRAELESGRRLTTAMRNNHVDLAALESILHGPGKTTDTSSRNTLLTAESLKEIRGRLKKLSDESLYKDDFIAYQQPPSADEHIEVQQSVRRKLSTPRLQQLTPEESPEISTPTQHTSAFKVVGAQRKPTDNDSLTQRSTHLNQKHTSNSLESRQKQRDTNSTEWHSRRKSYGFEKMSPPDNKTMHRMDASTDSGLGRSGELGNWSPTEAAPPRATVIHFGEPAKAVTSAHYRAAKSPVRRTPDEDLLKRHSIAVDESQYVRDNLRKTSQVHLNGFYDPAEPAAKSTVQLSRYDSNAESNQLFQRNTQNAQKRVEFCKTEVHFAAESGRVNIVETDGKPPPTNNFRRRRRTTSGPLQSLTKTASTAGTSTAVSTTTASNVTHFGDDQHQQQRHRTIASSVVAYKATLVEPPTVLNDAQIMPATTNVTVSVQHAEGSRGSYASTTSGVDTTDNENDEIAAIRGILKNKPVKPKPYHLGENIDSADALWGVQLKPVAGGGSSAAREKEDSPTAVLNKSVAERVRIVEKRHEHNAPNGYSTKINLSLDEPAAAARDWPNADLPKSNIMSIELHSPQTSLEKRDSIKRNQTPPVAKPRTVTGAVNATQHPQIHVATTVKQTADRFSAAEQQRQSLKIVKEARGARKLREHELSYFGVPHGQKHEENETKPKQQPNSNANTLSLSKRSLPSRQRLTVIGNEDTKSMRRHEANNTETTTVSGQTQTATKWQLLNDKPDLLRHSPIAVEPQLIRRTADVQRSERNSTSVERNKRMPSKELRLDDELNDAVEDDDDDNEHFYENITNEITPVFKVKSPQPYDRKMDMERDAFILNEMNENADLTMKALSDEAAHKDRRRRRSSLHRRDSKPLETIEEKLVTQKPNDTCIAVKMSRGTLASEAKRHARHTTTSPSPHDTTTRVRTSSQSSVESCPRARSRSLSSEREYENIRTPKPTRTNATATTKIQAKSVSKQQQRSSSRESQRSARLPYSSGDEVNPREEQRVRMKSKRSSNSTSGTTGRSGERQRSSKKDSHHDKHGHHTTTTSSRDVEQRRRAASSTTHASQREERGECVTVPQQQRTASSGRSSSSNKRREEPATTAGSSGRHSSSRNATEKARTSAGVRLLRSLRVGRTDDAKASSTEQRASGHHRSTSEREKAHEKEREKELQSKSRGHSTSKTQHHSSSEHHQHSLTAHSGTMSSKTREHKKATSNAIEKPHERAITPTYVYETETNLPHIQTDSNSSESLVQTQTTIAAMTHSTTHECHENYARLKSELKTHTNAATQHASLTATQYDYCTEVAPLPPPRTRRKRHRSLIPIPINPSTPTPTATTSPSPSSLPITYEHQIYAQIIPPVAYAPAQPTYSNQSTLRKCSLQRIMRRRSALPLPLTFPCARKPSQTQSTTSSFAFLPYLPAAPGKRPSDASHIYHNYLLYATPAKVEAEPLNLTQPADELATFWHADALHRPERPQRLRTMRSHVSTHHPFALCTCS

Target ORF information:

RefSeq Version	XM_011178365.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178365.1

ORF Insert Sequence:

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGA TCTACCTAAA TCGAACATTA TGAGCATAGA ACTACATTCA 
CCACAAACTT CGTTGGAGAA ACGTGATTCC ATCAAACGCA ACCAAACACC GCCCGTGGCC 
AAACCGAGAA CTGTGACTGG TGCAGTCAAC GCCACTCAGC ATCCACAGAT ACATGTGGCC 
ACCACAGTCA AGCAAACAGC TGATCGTTTT TCAGCAGCCG AACAGCAACG CCAGTCACTG 
AAAATTGTCA AGGAAGCGCG TGGTGCACGT AAACTGCGTG AACACGAACT CTCATACTTT 
GGCGTACCAC ACGGACAGAA ACACGAAGAG AACGAAACAA AACCGAAGCA ACAGCCGAAT 
AGCAACGCTA ATACGTTGAG TTTATCGAAG CGTTCGCTAC CGTCCCGTCA ACGTTTAACT 
GTTATTGGTA ACGAGGATAC GAAATCGATG CGTCGACATG AAGCAAACAA CACTGAAACA 
ACAACCGTAA GCGGACAAAC ACAAACGGCC ACTAAATGGC AATTGTTGAA TGACAAACCC 
GATCTACTAC GACACAGTCC GATTGCTGTG GAACCACAAT TGATACGACG TACAGCAGAT 
GTGCAGCGTA GTGAACGTAA TAGCACATCC GTGGAACGTA ACAAACGTAT GCCCTCAAAA 
GAGTTGAGGT TAGATGATGA GCTCAATGAT GCTGTTGAAG ATGATGATGA CGACAACGAG 
CATTTTTATG AAAATATAAC AAATGAAATT ACGCCTGTCT TCAAAGTGAA GTCACCGCAA 
CCGTATGATC GTAAAATGGA TATGGAACGC GACGCATTCA TATTGAACGA GATGAATGAG 
AATGCCGATC TGACTATGAA GGCGCTCTCC GATGAGGCCG CCCATAAAGA TCGGCGACGA 
CGACGTTCAT CGCTCCATAG ACGCGACTCC AAACCACTAG AAACGATCGA AGAGAAATTG 
GTCACCCAAA AACCCAACGA CACATGCATT GCGGTGAAAA TGTCACGTGG CACGCTAGCC 
TCCGAAGCCA AACGCCACGC ACGTCACACA ACCACTTCAC CCAGCCCACA CGATACAACC 
ACACGAGTGC GTACTTCATC ACAATCGTCG GTAGAAAGTT GTCCGCGTGC ACGTTCGCGT 
TCACTCTCCA GCGAACGTGA GTACGAAAAC ATACGTACAC CGAAACCGAC GCGCACCAAC 
GCAACAGCCA CAACGAAAAT ACAAGCCAAA AGCGTTAGCA AACAACAGCA GCGCTCCAGT 
TCACGTGAAT CACAGCGTTC AGCGCGTTTG CCATATTCAT CGGGCGATGA GGTAAACCCA 
CGCGAGGAAC AACGTGTACG CATGAAGAGT AAACGTAGCT CGAACTCTAC GAGTGGCACA 
ACGGGTCGTA GTGGTGAACG ACAACGTAGC AGTAAGAAGG ATAGCCATCA CGACAAACAT 
GGACATCACA CGACAACAAC AAGCAGTCGA GATGTTGAGC AGCGACGGCG CGCGGCCAGC 
AGCACTACGC ATGCCTCTCA GCGAGAGGAA CGCGGTGAGT GCGTGACTGT GCCGCAACAA 
CAGCGCACCG CAAGTAGTGG ACGCAGCAGT AGCAGCAACA AGCGACGTGA AGAACCTGCC 
ACCACCGCTG GCAGTAGCGG ACGTCACTCC AGCTCACGCA ATGCCACGGA GAAGGCACGC 
ACTAGCGCTG GTGTGCGTCT ATTGCGCTCG CTACGCGTCG GACGTACGGA TGATGCCAAA 
GCGTCCAGCA CGGAGCAACG TGCGTCCGGA CATCATCGTT CCACCAGCGA GCGCGAGAAG 
GCACATGAGA AAGAGCGCGA AAAGGAGTTG CAGTCCAAAT CGCGAGGTCA CTCAACCAGC 
AAAACCCAGC ACCACAGCAG TAGTGAGCAC CACCAACATT CACTAACCGC ACATAGCGGT 
ACAATGTCCA GTAAAACACG CGAACACAAG AAAGCAACCT CAAACGCCAT TGAGAAGCCG 
CACGAACGAG CCATCACACC AACGTACGTA TACGAAACCG AAACGAATTT ACCACATATA 
CAAACCGATA GCAACAGTAG TGAGAGTTTA GTACAGACGC AAACCACAAT TGCTGCTATG 
ACGCACAGCA CAACACATGA ATGTCACGAA AATTATGCGC GACTGAAGTC AGAGCTTAAA 
ACGCATACAA ATGCTGCGAC ACAACATGCA AGCTTAACCG CAACACAATA TGACTATTGT 
ACGGAGGTGG CACCATTACC ACCGCCGCGC ACACGCCGCA AGCGTCATAG ATCACTCATA 
CCTATACCCA TAAATCCTAG TACACCAACG CCAACAGCAA CAACATCCCC ATCTCCATCC 
TCGTTGCCCA TTACATATGA ACATCAGATT TATGCACAAA TCATACCGCC TGTTGCGTAT 
GCACCCGCAC AACCCACATA TTCCAATCAG TCGACATTAC GTAAATGTTC ATTGCAACGC 
ATTATGCGTA GACGTAGCGC TTTACCCTTG CCCCTCACAT TCCCATGTGC GCGCAAACCA 
TCGCAAACAC AATCGACCAC CTCCTCATTC GCATTCCTAC CCTATTTACC AGCCGCGCCC 
GGTAAGCGGC CCTCAGACGC GAGTCATATT TATCATAATT ATTTATTGTA TGCAACGCCG 
GCGAAAGTTG AAGCAGAGCC GTTGAATTTG ACGCAACCAG CCGATGAGTT GGCGACATTT 
TGGCACGCCG ATGCGCTACA CCGACCGGAG CGGCCGCAGC GTTTGCGCAC AATGCGTAGT 
CATGTGTCGA CGCATCATCC GTTCGCATTG TGCACATGCT CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208490 ( XM_011178366.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176668.1 Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X7, mRNA.

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CloneID	OBa194184
Clone ID Related Accession (Same CDS sequence)	XM_011178366.1
Accession Version	XM_011178366.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105208490 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863673.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GAGCTGCTTG TTTTGGGCAT CTTAATAATG GGTGCTACAC AGCCGCAAAC ACTACACAAA 
ACTCTTAGGT CACTCAACCA GCAAAACCCA GCACCACAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176668.1
CDS	950..7234
Translation	MEVATTNTHHHNHHHQHLGHQLHHPAHRLPMAKSTQHQQQQQQSNHCLTVSNDSAQSGGGGGGGSPRSPFQREVREWQRIYPDTGALLSGRLEADRWINGPLNSYGKISDSQNISQPNGTQHTQRKQLEVLQKRTANGAVQVIRAQTVQTSSSRSWSSSNSNSASTSASASASASPICMQANVVGARSLLHNHTNNNINNINSSKHFHSTLQQHLAEANTHWLQLSPPTIAATVDISEVSTDDDLDKAVPTIAETPYIVDDSEIDVGNGCGVDADINASATLCNYSISRSGSAGSGGGGSSKGHALLSSAASSSSTLHSQSSALLVPDTAPSLKLSNLMKSSSGGSSSSNNSTKLNTHKSAGGKVSLHAIVGSAGDNNTNSGSASRINNDLTESPKSTTTTAKSSNEALSGTKTWQQQQRRSNARHLEQATLYGQNSNSSKMRVGGADCDDCYAVVSADADESQLRYHQHHLQPQQLRKQQQQQQQSSVVLSETTPPTRRRDLESFRLYGDSTTAATTAGAAADDNAKADDLRLSTRQLRRQQQPFGQLSSSALIQRSHSISTDDLSNEWESNEAVAESNEWRRVSKLRRSFQSSSTANNSNTSAPAVQQPTSSIRRSYDLPVSAVSVSRIRAELESGRRLTTAMRNNHVDLAALESILHGPGKTTDTSSRNTLLTAESLKEIRGRLKKLSDESLYKDDFIAYQQPPSADEHIEVQQSVRRKLSTPRLQQLTPEESPEISTPTQHTSAFKVVGAQRKPTDNDSLTQRSTHLNQKHTSNSLESRQKQRDTNSTEWHSRRKSYGFEKMSPPDNKTMHRMDASTDSGLGRSGELGNWSPTEAAPPRATVIHFGEPAKAVTSAHYRAAKSPVRRTPDEDLLKRHSIAVDESQYVRDNLRKTSQVHLNGFYDPAEPAAKSTVQLSRYDSNAESNQLFQRNTQNAQKRVEFCKTEVHFAAESGRVNIVETDGKPPPTNNFRRRRRTTSGPLQSLTKTASTAGTSTAVSTTTASNVTHFGDDQHQQQRHRTIASSVVAYKATLVEPPTVLNDAQIMPATTNVTVSVQHAEGSRGSYASTTSGVDTTDNENDEIAAIRGILKNKPVKPKPYHLGENIDSADALWGVQLKPVAGGGSSAAREKEDSPTAVLNKSVAERVRIVEKRHEHNAPNGYSTKINLSLDEPAAAARDWPNAGAPMLMPNTDNQPIPNNTNRRASAQELILQDLREHQRMLDEGLKSTSLIIKTMRSANEFDEAMRRLSIASLESSTMPPIVVPTPMLRSNSYQETVRRYSNASLESTERELTTQTVVHTSSSAAALTAATSSRRLSFETQSAFVTPLSLPPAPLVAPRLKLLGSTEVSVSQQLSQLRRMYDIAATQQENDEDAADSADEEVKSYFCAHEQAEQSDSGGGTLESSSQEDERAIEYSSGSWSRMKAKRTIWKIEAEERKAAPAVLDKADLPKSNIMSIELHSPQTSLEKRDSIKRNQTPPVAKPRTVTGAVNATQHPQIHVATTVKQTADRFSAAEQQRQSLKIVKEARGARKLREHELSYFGVPHGQKHEENETKPKQQPNSNANTLSLSKRSLPSRQRLTVIGNEDTKSMRRHEANNTETTTVSGQTQTATKWQLLNDKPDLLRHSPIAVEPQLIRRTADVQRSERNSTSVERNKRMPSKELRLDDELNDAVEDDDDDNEHFYENITNEITPVFKVKSPQPYDRKMDMERDAFILNEMNENADLTMKALSDEAAHKDRRRRRSSLHRRDSKPLETIEEKLVTQKPNDTCIAVKMSRGTLASEAKRHARHTTTSPSPHDTTTRVRTSSQSSVESCPRARSRSLSSEREYENIRTPKPTRTNATATTKIQAKSVSKQQQRSSSRESQRSARLPYSSGDEVNPREEQRVRMKSKRSSNSTSGTTGRSGERQRSSKKDSHHDKHGHHTTTTSSRDVEQRRRAASSTTHASQREERGECVTVPQQQRTASSGRSSSSNKRREEPATTAGSSGRHSSSRNATEKARTSAGVRLLRSLRVGRTDDAKASSTEQRASGHHRSTSEREKAHEKEREKELQSKSRELLVLGILIMGATQPQTLHKTLRSLNQQNPAPQQ

Target ORF information:

RefSeq Version	XM_011178366.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178366.1

ORF Insert Sequence:

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GAGCTGCTTG TTTTGGGCAT CTTAATAATG GGTGCTACAC AGCCGCAAAC ACTACACAAA 
ACTCTTAGGT CACTCAACCA GCAAAACCCA GCACCACAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208490 ( XM_011178367.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176669.1 Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X7, mRNA.

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CloneID	OBa194185
Clone ID Related Accession (Same CDS sequence)	XM_011178367.2 , XM_011178367.1
Accession Version	XM_011178367.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105208490 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863673.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011178367.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GACACAGTAA AAGCTCGCAA AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176669.1
CDS	948..7154
Translation	MEVATTNTHHHNHHHQHLGHQLHHPAHRLPMAKSTQHQQQQQQSNHCLTVSNDSAQSGGGGGGGSPRSPFQREVREWQRIYPDTGALLSGRLEADRWINGPLNSYGKISDSQNISQPNGTQHTQRKQLEVLQKRTANGAVQVIRAQTVQTSSSRSWSSSNSNSASTSASASASASPICMQANVVGARSLLHNHTNNNINNINSSKHFHSTLQQHLAEANTHWLQLSPPTIAATVDISEVSTDDDLDKAVPTIAETPYIVDDSEIDVGNGCGVDADINASATLCNYSISRSGSAGSGGGGSSKGHALLSSAASSSSTLHSQSSALLVPDTAPSLKLSNLMKSSSGGSSSSNNSTKLNTHKSAGGKVSLHAIVGSAGDNNTNSGSASRINNDLTESPKSTTTTAKSSNEALSGTKTWQQQQRRSNARHLEQATLYGQNSNSSKMRVGGADCDDCYAVVSADADESQLRYHQHHLQPQQLRKQQQQQQQSSVVLSETTPPTRRRDLESFRLYGDSTTAATTAGAAADDNAKADDLRLSTRQLRRQQQPFGQLSSSALIQRSHSISTDDLSNEWESNEAVAESNEWRRVSKLRRSFQSSSTANNSNTSAPAVQQPTSSIRRSYDLPVSAVSVSRIRAELESGRRLTTAMRNNHVDLAALESILHGPGKTTDTSSRNTLLTAESLKEIRGRLKKLSDESLYKDDFIAYQQPPSADEHIEVQQSVRRKLSTPRLQQLTPEESPEISTPTQHTSAFKVVGAQRKPTDNDSLTQRSTHLNQKHTSNSLESRQKQRDTNSTEWHSRRKSYGFEKMSPPDNKTMHRMDASTDSGLGRSGELGNWSPTEAAPPRATVIHFGEPAKAVTSAHYRAAKSPVRRTPDEDLLKRHSIAVDESQYVRDNLRKTSQVHLNGFYDPAEPAAKSTVQLSRYDSNAESNQLFQRNTQNAQKRVEFCKTEVHFAAESGRVNIVETDGKPPPTNNFRRRRRTTSGPLQSLTKTASTAGTSTAVSTTTASNVTHFGDDQHQQQRHRTIASSVVAYKATLVEPPTVLNDAQIMPATTNVTVSVQHAEGSRGSYASTTSGVDTTDNENDEIAAIRGILKNKPVKPKPYHLGENIDSADALWGVQLKPVAGGGSSAAREKEDSPTAVLNKSVAERVRIVEKRHEHNAPNGYSTKINLSLDEPAAAARDWPNAGAPMLMPNTDNQPIPNNTNRRASAQELILQDLREHQRMLDEGLKSTSLIIKTMRSANEFDEAMRRLSIASLESSTMPPIVVPTPMLRSNSYQETVRRYSNASLESTERELTTQTVVHTSSSAAALTAATSSRRLSFETQSAFVTPLSLPPAPLVAPRLKLLGSTEVSVSQQLSQLRRMYDIAATQQENDEDAADSADEEVKSYFCAHEQAEQSDSGGGTLESSSQEDERAIEYSSGSWSRMKAKRTIWKIEAEERKAAPAVLDKADLPKSNIMSIELHSPQTSLEKRDSIKRNQTPPVAKPRTVTGAVNATQHPQIHVATTVKQTADRFSAAEQQRQSLKIVKEARGARKLREHELSYFGVPHGQKHEENETKPKQQPNSNANTLSLSKRSLPSRQRLTVIGNEDTKSMRRHEANNTETTTVSGQTQTATKWQLLNDKPDLLRHSPIAVEPQLIRRTADVQRSERNSTSVERNKRMPSKELRLDDELNDAVEDDDDDNEHFYENITNEITPVFKVKSPQPYDRKMDMERDAFILNEMNENADLTMKALSDEAAHKDRRRRRSSLHRRDSKPLETIEEKLVTQKPNDTCIAVKMSRGTLASEAKRHARHTTTSPSPHDTTTRVRTSSQSSVESCPRARSRSLSSEREYENIRTPKPTRTNATATTKIQAKSVSKQQQRSSSRESQRSARLPYSSGDEVNPREEQRVRMKSKRSSNSTSGTTGRSGERQRSSKKDSHHDKHGHHTTTTSSRDVEQRRRAASSTTHASQREERGECVTVPQQQRTASSGRSSSSNKRREEPATTAGSSGRHSSSRNATEKARTSAGVRLLRSLRVGRTDDAKASSTEQRASGHHRSTSEREKAHEKEREKELQSKSRDTVKARKT

Target ORF information:

RefSeq Version	XM_011178367.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178367.2

ORF Insert Sequence:

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GACACAGTAA AAGCTCGCAA AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208490 ( XM_011178367.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176669.1 Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X8, mRNA.

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CloneID	OBa194185
Clone ID Related Accession (Same CDS sequence)	XM_011178367.2 , XM_011178367.1
Accession Version	XM_011178367.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105208490 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863673.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GACACAGTAA AAGCTCGCAA AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176669.1
CDS	950..7156
Translation	MEVATTNTHHHNHHHQHLGHQLHHPAHRLPMAKSTQHQQQQQQSNHCLTVSNDSAQSGGGGGGGSPRSPFQREVREWQRIYPDTGALLSGRLEADRWINGPLNSYGKISDSQNISQPNGTQHTQRKQLEVLQKRTANGAVQVIRAQTVQTSSSRSWSSSNSNSASTSASASASASPICMQANVVGARSLLHNHTNNNINNINSSKHFHSTLQQHLAEANTHWLQLSPPTIAATVDISEVSTDDDLDKAVPTIAETPYIVDDSEIDVGNGCGVDADINASATLCNYSISRSGSAGSGGGGSSKGHALLSSAASSSSTLHSQSSALLVPDTAPSLKLSNLMKSSSGGSSSSNNSTKLNTHKSAGGKVSLHAIVGSAGDNNTNSGSASRINNDLTESPKSTTTTAKSSNEALSGTKTWQQQQRRSNARHLEQATLYGQNSNSSKMRVGGADCDDCYAVVSADADESQLRYHQHHLQPQQLRKQQQQQQQSSVVLSETTPPTRRRDLESFRLYGDSTTAATTAGAAADDNAKADDLRLSTRQLRRQQQPFGQLSSSALIQRSHSISTDDLSNEWESNEAVAESNEWRRVSKLRRSFQSSSTANNSNTSAPAVQQPTSSIRRSYDLPVSAVSVSRIRAELESGRRLTTAMRNNHVDLAALESILHGPGKTTDTSSRNTLLTAESLKEIRGRLKKLSDESLYKDDFIAYQQPPSADEHIEVQQSVRRKLSTPRLQQLTPEESPEISTPTQHTSAFKVVGAQRKPTDNDSLTQRSTHLNQKHTSNSLESRQKQRDTNSTEWHSRRKSYGFEKMSPPDNKTMHRMDASTDSGLGRSGELGNWSPTEAAPPRATVIHFGEPAKAVTSAHYRAAKSPVRRTPDEDLLKRHSIAVDESQYVRDNLRKTSQVHLNGFYDPAEPAAKSTVQLSRYDSNAESNQLFQRNTQNAQKRVEFCKTEVHFAAESGRVNIVETDGKPPPTNNFRRRRRTTSGPLQSLTKTASTAGTSTAVSTTTASNVTHFGDDQHQQQRHRTIASSVVAYKATLVEPPTVLNDAQIMPATTNVTVSVQHAEGSRGSYASTTSGVDTTDNENDEIAAIRGILKNKPVKPKPYHLGENIDSADALWGVQLKPVAGGGSSAAREKEDSPTAVLNKSVAERVRIVEKRHEHNAPNGYSTKINLSLDEPAAAARDWPNAGAPMLMPNTDNQPIPNNTNRRASAQELILQDLREHQRMLDEGLKSTSLIIKTMRSANEFDEAMRRLSIASLESSTMPPIVVPTPMLRSNSYQETVRRYSNASLESTERELTTQTVVHTSSSAAALTAATSSRRLSFETQSAFVTPLSLPPAPLVAPRLKLLGSTEVSVSQQLSQLRRMYDIAATQQENDEDAADSADEEVKSYFCAHEQAEQSDSGGGTLESSSQEDERAIEYSSGSWSRMKAKRTIWKIEAEERKAAPAVLDKADLPKSNIMSIELHSPQTSLEKRDSIKRNQTPPVAKPRTVTGAVNATQHPQIHVATTVKQTADRFSAAEQQRQSLKIVKEARGARKLREHELSYFGVPHGQKHEENETKPKQQPNSNANTLSLSKRSLPSRQRLTVIGNEDTKSMRRHEANNTETTTVSGQTQTATKWQLLNDKPDLLRHSPIAVEPQLIRRTADVQRSERNSTSVERNKRMPSKELRLDDELNDAVEDDDDDNEHFYENITNEITPVFKVKSPQPYDRKMDMERDAFILNEMNENADLTMKALSDEAAHKDRRRRRSSLHRRDSKPLETIEEKLVTQKPNDTCIAVKMSRGTLASEAKRHARHTTTSPSPHDTTTRVRTSSQSSVESCPRARSRSLSSEREYENIRTPKPTRTNATATTKIQAKSVSKQQQRSSSRESQRSARLPYSSGDEVNPREEQRVRMKSKRSSNSTSGTTGRSGERQRSSKKDSHHDKHGHHTTTTSSRDVEQRRRAASSTTHASQREERGECVTVPQQQRTASSGRSSSSNKRREEPATTAGSSGRHSSSRNATEKARTSAGVRLLRSLRVGRTDDAKASSTEQRASGHHRSTSEREKAHEKEREKELQSKSRDTVKARKT

Target ORF information:

RefSeq Version	XM_011178367.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105208490 (LOC105208490), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178367.1

ORF Insert Sequence:

ATGGAAGTAG CAACAACAAA TACACATCAT CACAATCATC ATCATCAGCA TCTTGGCCAT 
CAACTACATC ATCCAGCGCA TCGTTTGCCA ATGGCCAAAT CAACGCAACA CCAACAACAA 
CAGCAACAAA GCAATCATTG CCTCACAGTG AGCAATGACA GCGCGCAGAG TGGCGGCGGC 
GGCGGCGGCG GTTCACCGCG TTCCCCCTTC CAGCGTGAGG TGCGCGAATG GCAACGCATC 
TATCCAGATA CGGGCGCTTT GCTGAGCGGC CGTTTAGAGG CCGATCGTTG GATTAATGGG 
CCGTTGAATA GTTACGGAAA GATATCCGAT TCCCAAAACA TCTCACAGCC GAACGGCACC 
CAACACACAC AGCGCAAACA GCTCGAAGTG CTGCAGAAGC GCACCGCCAA TGGGGCCGTG 
CAAGTTATAC GCGCACAAAC CGTACAAACA TCCTCATCCC GCTCATGGTC CTCGTCCAAT 
TCCAATTCCG CCTCGACATC TGCCTCGGCT TCCGCCTCAG CATCGCCCAT TTGCATGCAA 
GCGAATGTTG TTGGTGCGCG TAGCTTATTG CATAATCACA CCAACAACAA CATTAATAAC 
ATAAATAGCA GCAAACATTT TCATAGCACA TTGCAACAAC ATTTAGCCGA AGCAAACACT 
CATTGGCTGC AACTATCACC GCCAACAATT GCCGCCACTG TGGACATCAG CGAAGTCAGT 
ACGGACGATG ATCTCGATAA GGCAGTGCCA ACAATTGCCG AAACCCCCTA CATTGTTGAC 
GATAGCGAGA TCGATGTTGG CAATGGTTGT GGCGTTGACG CTGACATCAA CGCCAGCGCA 
ACGCTATGCA ATTATTCAAT TAGTCGCAGT GGCAGCGCCG GCAGTGGTGG TGGCGGCAGC 
AGCAAAGGTC ATGCTTTGTT GTCATCGGCA GCGTCGTCAT CATCAACATT GCATTCGCAA 
TCATCAGCGT TGCTGGTGCC CGATACGGCG CCAAGCTTGA AATTGAGTAA TCTTATGAAA 
AGCAGCAGCG GCGGCAGCAG CAGCAGCAAC AACTCCACAA AGCTGAACAC CCACAAGAGT 
GCTGGCGGTA AGGTGAGCCT ACATGCAATT GTTGGCAGCG CTGGTGACAA CAACACCAAC 
AGCGGCAGCG CTAGTCGAAT CAACAACGAC TTAACAGAGT CACCAAAAAG CACAACAACA 
ACAGCGAAAA GTTCGAATGA AGCATTGAGT GGTACAAAAA CGTGGCAGCA GCAGCAGCGG 
CGTAGCAACG CTCGTCATTT GGAACAAGCT ACTCTATATG GCCAAAACAG CAATTCATCT 
AAAATGCGCG TAGGCGGCGC TGATTGTGAT GATTGTTATG CGGTTGTGTC GGCTGACGCA 
GATGAGTCGC AGCTGCGCTA CCACCAACAC CACCTTCAAC CGCAACAGCT ACGTAAACAA 
CAACAACAGC AGCAACAAAG CAGTGTTGTG CTAAGTGAAA CGACACCACC AACGCGTCGA 
CGTGACTTGG AAAGTTTCCG GCTTTACGGT GACTCCACAA CAGCAGCGAC GACAGCGGGC 
GCGGCAGCGG ATGACAACGC CAAAGCCGAT GATTTACGTC TATCCACGCG TCAATTACGT 
CGACAACAAC AACCATTTGG TCAACTGTCC AGCAGCGCTT TAATCCAACG TTCCCATTCA 
ATTTCGACGG ACGATCTCAG TAACGAGTGG GAGTCGAACG AGGCGGTTGC TGAGTCCAAC 
GAGTGGCGAC GCGTCAGCAA ATTGCGACGC TCCTTTCAAT CATCCTCTAC GGCGAACAAT 
AGTAATACCA GCGCTCCCGC AGTGCAACAA CCGACGAGCT CTATACGCCG TTCATACGAT 
CTGCCCGTCA GTGCTGTGAG CGTGTCACGC ATACGCGCCG AACTCGAAAG CGGTCGACGT 
CTTACCACAG CAATGCGTAA TAATCATGTG GATTTGGCTG CACTAGAGAG TATACTACAC 
GGTCCAGGCA AAACTACGGA CACCAGTAGC CGTAATACAT TGCTTACCGC CGAGTCATTG 
AAAGAAATAC GTGGCCGTCT CAAGAAATTA TCGGATGAAT CATTGTATAA GGACGATTTT 
ATAGCATATC AACAACCGCC GTCAGCAGAC GAGCACATTG AAGTGCAGCA GAGCGTGCGT 
CGTAAACTGT CCACACCACG TTTGCAACAA TTGACACCGG AAGAGTCGCC CGAGATCTCA 
ACGCCAACAC AGCATACATC TGCCTTCAAA GTTGTGGGCG CACAACGCAA GCCAACAGAC 
AACGACAGCT TAACGCAGCG CTCAACGCAC TTGAACCAAA AACACACCTC AAACAGCTTG 
GAATCACGTC AAAAGCAAAG AGATACCAAT TCAACCGAGT GGCACTCGCG TCGCAAATCA 
TATGGTTTCG AGAAGATGTC ACCGCCGGAC AACAAAACTA TGCACCGCAT GGACGCATCC 
ACCGATAGCG GACTCGGACG TTCCGGCGAA CTGGGCAATT GGTCACCCAC GGAAGCAGCA 
CCACCGCGCG CCACAGTCAT ACATTTCGGC GAGCCCGCAA AAGCAGTCAC TTCGGCGCAC 
TACCGCGCCG CTAAATCCCC AGTGCGCCGC ACACCTGACG AGGACTTGCT CAAGCGACAC 
TCGATCGCTG TCGATGAGAG TCAATATGTG CGCGATAATT TGCGTAAAAC CTCACAGGTG 
CATTTGAATG GTTTCTATGA TCCGGCCGAG CCCGCTGCCA AATCCACAGT GCAGTTGAGC 
CGTTATGACA GCAATGCGGA GAGTAATCAA CTCTTCCAAC GCAACACACA AAATGCACAG 
AAACGTGTTG AGTTCTGCAA AACCGAGGTA CATTTCGCCG CCGAATCGGG GCGTGTCAAT 
ATCGTTGAAA CCGATGGTAA ACCGCCACCG ACAAATAATT TCCGTCGACG CCGTCGCACC 
ACAAGTGGAC CGCTACAGAG TCTTACCAAA ACCGCAAGCA CGGCGGGTAC AAGCACAGCC 
GTGAGCACAA CAACCGCTAG TAATGTTACA CATTTCGGTG ATGATCAACA TCAGCAGCAA 
CGTCATCGCA CCATCGCCAG TTCGGTGGTC GCATATAAAG CTACATTAGT GGAACCACCA 
ACTGTGCTCA ATGACGCACA AATAATGCCG GCCACAACTA ACGTAACGGT ATCCGTACAA 
CATGCGGAGG GTTCACGTGG CAGCTACGCG TCAACAACAA GCGGTGTCGA TACGACAGAC 
AATGAAAATG ACGAAATCGC CGCTATACGC GGCATACTCA AGAATAAACC GGTAAAGCCG 
AAACCTTATC ATTTAGGTGA GAACATCGAT AGCGCCGATG CTTTGTGGGG TGTGCAGTTG 
AAGCCTGTGG CTGGTGGCGG CAGCAGCGCA GCGCGTGAGA AAGAAGACTC ACCCACTGCT 
GTGTTGAATA AATCCGTTGC GGAACGCGTG CGCATTGTGG AGAAACGTCA TGAGCATAAC 
GCTCCAAATG GTTACTCTAC GAAAATCAAC TTGAGTCTGG ACGAACCAGC TGCAGCAGCG 
CGTGATTGGC CTAATGCAGG TGCGCCTATG CTAATGCCAA ACACTGACAA TCAACCCATA 
CCCAACAACA CAAATCGCCG TGCGAGCGCA CAAGAGCTCA TATTACAAGA TCTACGCGAA 
CATCAGCGTA TGCTCGACGA GGGACTCAAG TCTACATCGC TCATCATCAA AACAATGCGT 
TCGGCAAATG AGTTTGATGA GGCTATGCGG CGTCTAAGTA TAGCTTCACT GGAATCGAGT 
ACGATGCCAC CAATTGTGGT GCCCACACCA ATGTTGCGTT CGAATAGCTA CCAGGAGACG 
GTACGTCGTT ACTCCAACGC CTCCTTGGAG AGCACCGAAC GCGAACTTAC CACACAGACT 
GTAGTTCATA CGAGTAGCAG TGCTGCAGCG TTAACAGCGG CCACAAGTAG TCGCAGATTG 
TCGTTTGAAA CACAAAGCGC TTTCGTAACA CCGTTAAGCC TACCACCAGC ACCGCTAGTG 
GCACCACGTC TAAAGCTGCT CGGCAGCACT GAGGTATCAG TGAGTCAGCA GCTTTCACAG 
TTGCGACGCA TGTACGATAT TGCAGCGACA CAGCAAGAAA ATGACGAAGA TGCCGCCGAC 
AGTGCCGATG AAGAGGTAAA AAGCTACTTT TGCGCACACG AACAAGCGGA ACAAAGCGAT 
AGTGGTGGCG GCACGTTGGA GAGCTCTTCG CAGGAAGATG AGCGCGCCAT TGAATACTCC 
AGCGGCAGTT GGAGTCGCAT GAAGGCGAAG CGCACAATTT GGAAGATTGA AGCGGAAGAA 
CGTAAAGCGG CGCCGGCCGT ATTGGACAAA GCAGATCTAC CTAAATCGAA CATTATGAGC 
ATAGAACTAC ATTCACCACA AACTTCGTTG GAGAAACGTG ATTCCATCAA ACGCAACCAA 
ACACCGCCCG TGGCCAAACC GAGAACTGTG ACTGGTGCAG TCAACGCCAC TCAGCATCCA 
CAGATACATG TGGCCACCAC AGTCAAGCAA ACAGCTGATC GTTTTTCAGC AGCCGAACAG 
CAACGCCAGT CACTGAAAAT TGTCAAGGAA GCGCGTGGTG CACGTAAACT GCGTGAACAC 
GAACTCTCAT ACTTTGGCGT ACCACACGGA CAGAAACACG AAGAGAACGA AACAAAACCG 
AAGCAACAGC CGAATAGCAA CGCTAATACG TTGAGTTTAT CGAAGCGTTC GCTACCGTCC 
CGTCAACGTT TAACTGTTAT TGGTAACGAG GATACGAAAT CGATGCGTCG ACATGAAGCA 
AACAACACTG AAACAACAAC CGTAAGCGGA CAAACACAAA CGGCCACTAA ATGGCAATTG 
TTGAATGACA AACCCGATCT ACTACGACAC AGTCCGATTG CTGTGGAACC ACAATTGATA 
CGACGTACAG CAGATGTGCA GCGTAGTGAA CGTAATAGCA CATCCGTGGA ACGTAACAAA 
CGTATGCCCT CAAAAGAGTT GAGGTTAGAT GATGAGCTCA ATGATGCTGT TGAAGATGAT 
GATGACGACA ACGAGCATTT TTATGAAAAT ATAACAAATG AAATTACGCC TGTCTTCAAA 
GTGAAGTCAC CGCAACCGTA TGATCGTAAA ATGGATATGG AACGCGACGC ATTCATATTG 
AACGAGATGA ATGAGAATGC CGATCTGACT ATGAAGGCGC TCTCCGATGA GGCCGCCCAT 
AAAGATCGGC GACGACGACG TTCATCGCTC CATAGACGCG ACTCCAAACC ACTAGAAACG 
ATCGAAGAGA AATTGGTCAC CCAAAAACCC AACGACACAT GCATTGCGGT GAAAATGTCA 
CGTGGCACGC TAGCCTCCGA AGCCAAACGC CACGCACGTC ACACAACCAC TTCACCCAGC 
CCACACGATA CAACCACACG AGTGCGTACT TCATCACAAT CGTCGGTAGA AAGTTGTCCG 
CGTGCACGTT CGCGTTCACT CTCCAGCGAA CGTGAGTACG AAAACATACG TACACCGAAA 
CCGACGCGCA CCAACGCAAC AGCCACAACG AAAATACAAG CCAAAAGCGT TAGCAAACAA 
CAGCAGCGCT CCAGTTCACG TGAATCACAG CGTTCAGCGC GTTTGCCATA TTCATCGGGC 
GATGAGGTAA ACCCACGCGA GGAACAACGT GTACGCATGA AGAGTAAACG TAGCTCGAAC 
TCTACGAGTG GCACAACGGG TCGTAGTGGT GAACGACAAC GTAGCAGTAA GAAGGATAGC 
CATCACGACA AACATGGACA TCACACGACA ACAACAAGCA GTCGAGATGT TGAGCAGCGA 
CGGCGCGCGG CCAGCAGCAC TACGCATGCC TCTCAGCGAG AGGAACGCGG TGAGTGCGTG 
ACTGTGCCGC AACAACAGCG CACCGCAAGT AGTGGACGCA GCAGTAGCAG CAACAAGCGA 
CGTGAAGAAC CTGCCACCAC CGCTGGCAGT AGCGGACGTC ACTCCAGCTC ACGCAATGCC 
ACGGAGAAGG CACGCACTAG CGCTGGTGTG CGTCTATTGC GCTCGCTACG CGTCGGACGT 
ACGGATGATG CCAAAGCGTC CAGCACGGAG CAACGTGCGT CCGGACATCA TCGTTCCACC 
AGCGAGCGCG AGAAGGCACA TGAGAAAGAG CGCGAAAAGG AGTTGCAGTC CAAATCGCGA 
GACACAGTAA AAGCTCGCAA AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC105208490 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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