sppl2b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	sppl2b
Entrez Gene ID	780336
Full Name	signal peptide peptidase like 2B
General protein information	Preferred Names signal peptide peptidase like 2B Names signal peptide peptidase-like 2B
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012966166.2	XP_012821620.1	signal peptide peptidase-like 2B isoform X2
NM_001079406.1	NP_001072874.1	signal peptide peptidase-like 2B precursor
XM_012966156.3	XP_012821610.2	signal peptide peptidase-like 2B isoform X1
XM_012966156.2	XP_012821610.1	signal peptide peptidase-like 2B isoform X1
XM_012966162.2	XP_012821616.1	signal peptide peptidase-like 2B isoform X2
XM_012966162.3	XP_012821616.2	signal peptide peptidase-like 2B isoform X2
XM_012966166.3	XP_012821620.2	signal peptide peptidase-like 2B isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	sppl2	NP_001015067.1
Homo sapiens (human)	SPPL2B	NP_694533.1
Canis lupus familiaris (dog)	SPPL2B	XP_542189.4
Mus musculus (house mouse)	Sppl2b	NP_780404.2
Rattus norvegicus (Norway rat)	Sppl2b	NP_001014222.1
Arabidopsis thaliana (thale cress)	SPPL5	NP_172073.2
Xenopus tropicalis (tropical clawed frog)	sppl2b	NP_001072874.1
Pan troglodytes (chimpanzee)	SPPL2B	XP_003316040.2
Bos taurus (cattle)	SPPL2B	XP_002689000.2
Arabidopsis thaliana (thale cress)	SPPL3	NP_850383.2
Oryza sativa (rice)	Os01g0914700	NP_001045182.1
Gallus gallus (chicken)	SPPL2B	NP_001026104.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sppl2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sppl2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa25338	NM_001079406.1 Latest version!	Xenopus tropicalis signal peptide peptidase like 2B (sppl2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa29693	XM_012966156.2 Latest version!	Xenopus tropicalis signal peptide peptidase like 2B (sppl2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa29694	XM_012966166.2 Latest version!	Xenopus tropicalis signal peptide peptidase like 2B (sppl2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa29694	XM_012966162.2 Latest version!	Xenopus tropicalis signal peptide peptidase like 2B (sppl2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa48079	XM_012966156.3 Latest version!	Xenopus tropicalis signal peptide peptidase like 2B (sppl2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa48080	XM_012966162.3 Latest version!	Xenopus tropicalis signal peptide peptidase like 2B (sppl2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa48080	XM_012966166.3 Latest version!	Xenopus tropicalis signal peptide peptidase like 2B (sppl2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sppl2b ( NM_001079406.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001072874.1 Xenopus tropicalis signal peptide peptidase like 2B (sppl2b), mRNA.

Price & Availability↑

CloneID	OXa25338
Clone ID Related Accession (Same CDS sequence)	NM_001079406.1
Accession Version	NM_001079406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Xenopus tropicalis(tropical clawed frog)
Product	signal peptide peptidase-like 2B precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC124023.1. ##Evidence-Data-START## Transcript exon combination :: BC124023.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCTTC TTTTGATTGT CCTGTTATGG GGTTTGTTTG TAACAGAGGT GGTGGGTGAA 
TACGGAATGG TTCATGTCAC ATCGGAAAAG AGCGGCTCCA GTTCCAAAGA TTACTGCATT 
TTGTTTAATT CCCAGTGGGC TCACCTTCCC CAAGACTTGG CCAAAGCTGG CCTCTTGCAA 
CTCCAGAATG AGACTGAATC TGCATTATGT TCCCCTTCAG ATGTGCCAGA AGGGGGATTC 
TTTAACCGCA TCCCAATGGT CATGCGAGGG AACTGCACAT TTTACGAGAA AGTGCGATTG 
GCACAGGTTA ATGGAGCTAA GGGTCTGCTA ATTGTCAGCA GAGAAAGACT GGTACCACCA 
GGGGGTAATC AGAGTCAGTT TGAAGAAATA GATATTCCTG TGGCTCTCCT TAGCTACAGT 
GACATGCTGG ATATTGGCAA AACATTTGGA AAGTCTGTGA AAGTGGCTAT GTATGCACCA 
AATGAACCTG TGCTGGACTA CAACATGGTC ATTATCTTTT TGATGGCTGT TGGAACTGTT 
GCAGTTGGTG GTTATTGGGC AGGAAGCCGT GATGTTAAAA AAAGGTACAT GAAGCACAAG 
AGAGATGATG GATCAGATAA AAAGCAAGAT GATGAGACTG TGGATGTTAC CCCTATAATG 
ATCTGTGTAT TTGTGGTTAT GTGTTGCTCA ATGCTGATTT TGCTGTATTA CTTTTACGAT 
CACTTAGTGT ATGTGATCAT TGGAATATTT TGCCTTGCTG CATCGATTGG TCTATATAGT 
TGTCTGTCTC CATTTGTCCG CAGATTTCCT TTTGGAAAAT GCAGGGTGCC AGATAATAAC 
CTGCCTTACT TTCACAAGCG ACCGCCTTTT TGGAAGCTAC TGCTGGCTGC TTTCTGTATT 
GTGGTCAGTG TTATCTGGGG AGTATACAGA AATGAAGATC AGTGGGCCTG GGTTCTTCAA 
GATATACTTG GAATAGCATT TTGCCTCTAC ATGCTGAAGA CAATCAGAAT GCCCACATTT 
AAGGGCTGCA CTTTGCTTTT ATTTGTGCTC TTTATTTATG ATGTGTTCTT TGTCTTCATC 
ACTCCGTACC TCACCAAGCG TGGGGAGAGC ATCATGGTGG AAGTTGCCTC TGGGCCATCA 
AATTCTACTA CCCAGGAAAA GCTTCCAATG GTCCTTAAAG TGCCAAGGTT AAATTCCTCG 
CCCCTAGCTC TCTGTGACAG ACCATTCTCT CTCTTGGGCT TTGGGGATAT CTTGGTACCA 
GGACTGCTTG TTGCCTACTG TCACAGGTTT GATATCCAGG TACAATCATC AAGGATTTAC 
TTTGTTGCCT GCACTATAGC CTATGGAATA GGCCTTCTGG TGACTTTTGT AGCACTGGCA 
TTAATGCAAA AGGGACAGCC TGCTCTGCTC TATCTCGTCC CCTGTACCCT TCTTACCAGC 
CTTGCAGTAG CACTCTGGCG CAAGGAATTA CACATGTTCT GGACCGGAAG CGGATTTGTG 
CAGAAAGACC TTCCTCACCC CCCTTTAGTA GTGGAACCAA GTGGCTCTGA TGCATGCTTA 
GGAAAGAAGA CAGAGGATCA AGAGCATGAG GAAGACCCAA ACAGATGCCC TGCCGAGCCA 
CATCATTTTT CAGATCTAGA GACATCTAAA CTTGATGAGC AACAGGATAG AGCATTGTCT 
CAGGCTGACC AGGTTGTTTA CCCAGACACA AATCATCAGA GATCATCCGA TATGGACATT 
ACGGGAACAG TTCCTGAATT GCCAGAGCCC TCATCAGATG GTGAAATAAA CATTTCAGAT 
ACCTCTGTAT CCACAGAGTT ACAGTCTACC TCTGCAGTAC ATGGAGAAAC ACCAACCATG 
CAAAAGAGCC ATCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072874.1
CDS	43..1920
Translation	MKLLLIVLLWGLFVTEVVGEYGMVHVTSEKSGSSSKDYCILFNSQWAHLPQDLAKAGLLQLQNETESALCSPSDVPEGGFFNRIPMVMRGNCTFYEKVRLAQVNGAKGLLIVSRERLVPPGGNQSQFEEIDIPVALLSYSDMLDIGKTFGKSVKVAMYAPNEPVLDYNMVIIFLMAVGTVAVGGYWAGSRDVKKRYMKHKRDDGSDKKQDDETVDVTPIMICVFVVMCCSMLILLYYFYDHLVYVIIGIFCLAASIGLYSCLSPFVRRFPFGKCRVPDNNLPYFHKRPPFWKLLLAAFCIVVSVIWGVYRNEDQWAWVLQDILGIAFCLYMLKTIRMPTFKGCTLLLFVLFIYDVFFVFITPYLTKRGESIMVEVASGPSNSTTQEKLPMVLKVPRLNSSPLALCDRPFSLLGFGDILVPGLLVAYCHRFDIQVQSSRIYFVACTIAYGIGLLVTFVALALMQKGQPALLYLVPCTLLTSLAVALWRKELHMFWTGSGFVQKDLPHPPLVVEPSGSDACLGKKTEDQEHEEDPNRCPAEPHHFSDLETSKLDEQQDRALSQADQVVYPDTNHQRSSDMDITGTVPELPEPSSDGEINISDTSVSTELQSTSAVHGETPTMQKSHQ

Target ORF information:

RefSeq Version	NM_001079406.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis signal peptide peptidase like 2B (sppl2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079406.1

ORF Insert Sequence:

ATGAAGCTTC TTTTGATTGT CCTGTTATGG GGTTTGTTTG TAACAGAGGT GGTGGGTGAA 
TACGGAATGG TTCATGTCAC ATCGGAAAAG AGCGGCTCCA GTTCCAAAGA TTACTGCATT 
TTGTTTAATT CCCAGTGGGC TCACCTTCCC CAAGACTTGG CCAAAGCTGG CCTCTTGCAA 
CTCCAGAATG AGACTGAATC TGCATTATGT TCCCCTTCAG ATGTGCCAGA AGGGGGATTC 
TTTAACCGCA TCCCAATGGT CATGCGAGGG AACTGCACAT TTTACGAGAA AGTGCGATTG 
GCACAGGTTA ATGGAGCTAA GGGTCTGCTA ATTGTCAGCA GAGAAAGACT GGTACCACCA 
GGGGGTAATC AGAGTCAGTT TGAAGAAATA GATATTCCTG TGGCTCTCCT TAGCTACAGT 
GACATGCTGG ATATTGGCAA AACATTTGGA AAGTCTGTGA AAGTGGCTAT GTATGCACCA 
AATGAACCTG TGCTGGACTA CAACATGGTC ATTATCTTTT TGATGGCTGT TGGAACTGTT 
GCAGTTGGTG GTTATTGGGC AGGAAGCCGT GATGTTAAAA AAAGGTACAT GAAGCACAAG 
AGAGATGATG GATCAGATAA AAAGCAAGAT GATGAGACTG TGGATGTTAC CCCTATAATG 
ATCTGTGTAT TTGTGGTTAT GTGTTGCTCA ATGCTGATTT TGCTGTATTA CTTTTACGAT 
CACTTAGTGT ATGTGATCAT TGGAATATTT TGCCTTGCTG CATCGATTGG TCTATATAGT 
TGTCTGTCTC CATTTGTCCG CAGATTTCCT TTTGGAAAAT GCAGGGTGCC AGATAATAAC 
CTGCCTTACT TTCACAAGCG ACCGCCTTTT TGGAAGCTAC TGCTGGCTGC TTTCTGTATT 
GTGGTCAGTG TTATCTGGGG AGTATACAGA AATGAAGATC AGTGGGCCTG GGTTCTTCAA 
GATATACTTG GAATAGCATT TTGCCTCTAC ATGCTGAAGA CAATCAGAAT GCCCACATTT 
AAGGGCTGCA CTTTGCTTTT ATTTGTGCTC TTTATTTATG ATGTGTTCTT TGTCTTCATC 
ACTCCGTACC TCACCAAGCG TGGGGAGAGC ATCATGGTGG AAGTTGCCTC TGGGCCATCA 
AATTCTACTA CCCAGGAAAA GCTTCCAATG GTCCTTAAAG TGCCAAGGTT AAATTCCTCG 
CCCCTAGCTC TCTGTGACAG ACCATTCTCT CTCTTGGGCT TTGGGGATAT CTTGGTACCA 
GGACTGCTTG TTGCCTACTG TCACAGGTTT GATATCCAGG TACAATCATC AAGGATTTAC 
TTTGTTGCCT GCACTATAGC CTATGGAATA GGCCTTCTGG TGACTTTTGT AGCACTGGCA 
TTAATGCAAA AGGGACAGCC TGCTCTGCTC TATCTCGTCC CCTGTACCCT TCTTACCAGC 
CTTGCAGTAG CACTCTGGCG CAAGGAATTA CACATGTTCT GGACCGGAAG CGGATTTGTG 
CAGAAAGACC TTCCTCACCC CCCTTTAGTA GTGGAACCAA GTGGCTCTGA TGCATGCTTA 
GGAAAGAAGA CAGAGGATCA AGAGCATGAG GAAGACCCAA ACAGATGCCC TGCCGAGCCA 
CATCATTTTT CAGATCTAGA GACATCTAAA CTTGATGAGC AACAGGATAG AGCATTGTCT 
CAGGCTGACC AGGTTGTTTA CCCAGACACA AATCATCAGA GATCATCCGA TATGGACATT 
ACGGGAACAG TTCCTGAATT GCCAGAGCCC TCATCAGATG GTGAAATAAA CATTTCAGAT 
ACCTCTGTAT CCACAGAGTT ACAGTCTACC TCTGCAGTAC ATGGAGAAAC ACCAACCATG 
CAAAAGAGCC ATCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sppl2b ( XM_012966156.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821610.1 Xenopus tropicalis signal peptide peptidase like 2B (sppl2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa29693
Clone ID Related Accession (Same CDS sequence)	XM_012966156.2
Accession Version	XM_012966156.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	signal peptide peptidase-like 2B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966156.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTTC TTTTGATTGT CCTGTTATGG GGTTTGTTTG TAACAGAGGT GGTGGGTGAA 
TACGGAATGG TTCATGTCAC ATCGGAAAAG AGCGGCTCCA GTTCCAAAGA TTACTGCATT 
TTGTTTAATT CCCAGTGGGC TCACCTTCCC CAAGACTTGG CCAAAGCTGG CCTCTTGCAA 
CTCCAGAATG AGACTGAATC TGCATTATGT TCCCCTTCAG ATGTGCCAGA AGGGGGATTC 
TTTAACCGCA TCCCAATGGT CATGCGAGGG AACTGCACAT TTTACGAGAA AGTGCGATTG 
GCACAGGTTA ATGGAGCTAA GGGTCTGCTA ATTGTCAGCA GAGAAAGACT GGTACCACCA 
GGGGGTAATC AGAGTCAGTT TGAAGAAATA GATATTCCTG TGGCTCTCCT TAGCTACAGT 
GACATGCTGG ATATTGGCAA AACATTTGGA AAGTCTGTGA AAGTGGCTAT GTATGCACCA 
AATGAACCTG TGCTGGACTA CAACATGGTC ATTATCTTTT TGATGGCTGT TGGAACTGTT 
GCAGTTGGTG GTTATTGGGC AGGAAGCCGT GATGTTAAAA AAAGGTACAT GAAGCACAAG 
AGAGATGATG GATCAGATAA AAAGCAAGAT GATGAGACTG TGGATGTTAC CCCTATAATG 
ATCTGTGTAT TTGTGGTTAT GTGTTGCTCA ATGCTGATTT TGCTGTATTA CTTTTACGAT 
CACTTAGTGT ATGTGATCAT TGGAATATTT TGCCTTGCTG CATCGATTGG TCTATATAGT 
TGTCTGTCTC CATTTGTCCG CAGATTTCCT TTTGGAAAAT GCAGGGTGCC AGATAATAAC 
CTGCCTTACT TTCACAAGCG ACCGCCTTTT TGGAAGCTAC TGCTGGCTGC TTTCTGTATT 
GTGGTCAGTG TTATCTGGGG AGTATACAGA AATGAAGATC AGTGGGCCTG GGTTCTTCAA 
GATATACTTG GAATAGCATT TTGCCTCTAC ATGCTGAAGA CAATCAGAAT GCCCACATTT 
AAGGGCTGCA CTTTGCTTTT ATTTGTGCTC TTTATTTATG ATGTGTTCTT TGTCTTCATC 
ACTCCGTACC TCACCAAGCG TGGGGAGAGC ATCATGGTGG AAGTTGCCTC TGGGCCATCA 
AATTCTACTA CCCAGGAAAA GCTTCCAATG GTCCTTAAAG TGCCAAGGTT AAATTCCTCG 
CCCCTAGCTC TCTGTGACAG ACCATTCTCT CTCTTGGGCT TTGGGGATAT CTTGGTACCA 
GGACTGCTTG TTGCCTACTG TCACAGGTTT GATATCCAGG TACAATCATC AAGGATTTAC 
TTTGTTGCCT GCACTATAGC CTATGGAATA GGCCTTCTGG TGACTTTTGT AGCACTGGCA 
TTAATGCAAA AGGGACAGCC TGCTCTGCTC TATCTCGTCC CCTGTACCCT TCTTACCAGC 
CTTGCAGTAG CACTCTGGCG CAAGGAATTA CACATGTTCT GGACCGGAAG CGGATTTGTG 
AAAGACCTTC CTCAGCCCCC TTTAGTAGTG GAACCAAGTG GCTCTGATGC ATGCTTAGGA 
AAGAAGACAG AGGATCAAGA GCATGAGGAA GACCCAAACA GATGCCCTGC CGAGCCACAT 
CATTTTTCAG ATCTAGAGAC ATCTAAACTT GATGAGCAAC AGGATAGAGC ATTGTCTCAG 
GCTGACCAGG TTGTTTACCC AGACACAAAT CATCAGAGAT CATCCGATAT GGACATTACG 
GGAACAGTTC CTGAATTGCC AGAGCCCTCA TCAGATGGTG AAATAAACAT TTCAGATACC 
TCTGTATCCA CAGAGTTACA GTCTACCTCT GCAGTACATG GAGAAACACC AACCATGCAA 
AAGAGCCATC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821610.1
CDS	214..2088
Translation	MKLLLIVLLWGLFVTEVVGEYGMVHVTSEKSGSSSKDYCILFNSQWAHLPQDLAKAGLLQLQNETESALCSPSDVPEGGFFNRIPMVMRGNCTFYEKVRLAQVNGAKGLLIVSRERLVPPGGNQSQFEEIDIPVALLSYSDMLDIGKTFGKSVKVAMYAPNEPVLDYNMVIIFLMAVGTVAVGGYWAGSRDVKKRYMKHKRDDGSDKKQDDETVDVTPIMICVFVVMCCSMLILLYYFYDHLVYVIIGIFCLAASIGLYSCLSPFVRRFPFGKCRVPDNNLPYFHKRPPFWKLLLAAFCIVVSVIWGVYRNEDQWAWVLQDILGIAFCLYMLKTIRMPTFKGCTLLLFVLFIYDVFFVFITPYLTKRGESIMVEVASGPSNSTTQEKLPMVLKVPRLNSSPLALCDRPFSLLGFGDILVPGLLVAYCHRFDIQVQSSRIYFVACTIAYGIGLLVTFVALALMQKGQPALLYLVPCTLLTSLAVALWRKELHMFWTGSGFVKDLPQPPLVVEPSGSDACLGKKTEDQEHEEDPNRCPAEPHHFSDLETSKLDEQQDRALSQADQVVYPDTNHQRSSDMDITGTVPELPEPSSDGEINISDTSVSTELQSTSAVHGETPTMQKSHQ

Target ORF information:

RefSeq Version	XM_012966156.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis signal peptide peptidase like 2B (sppl2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966156.2

ORF Insert Sequence:

ATGAAGCTTC TTTTGATTGT CCTGTTATGG GGTTTGTTTG TAACAGAGGT GGTGGGTGAA 
TACGGAATGG TTCATGTCAC ATCGGAAAAG AGCGGCTCCA GTTCCAAAGA TTACTGCATT 
TTGTTTAATT CCCAGTGGGC TCACCTTCCC CAAGACTTGG CCAAAGCTGG CCTCTTGCAA 
CTCCAGAATG AGACTGAATC TGCATTATGT TCCCCTTCAG ATGTGCCAGA AGGGGGATTC 
TTTAACCGCA TCCCAATGGT CATGCGAGGG AACTGCACAT TTTACGAGAA AGTGCGATTG 
GCACAGGTTA ATGGAGCTAA GGGTCTGCTA ATTGTCAGCA GAGAAAGACT GGTACCACCA 
GGGGGTAATC AGAGTCAGTT TGAAGAAATA GATATTCCTG TGGCTCTCCT TAGCTACAGT 
GACATGCTGG ATATTGGCAA AACATTTGGA AAGTCTGTGA AAGTGGCTAT GTATGCACCA 
AATGAACCTG TGCTGGACTA CAACATGGTC ATTATCTTTT TGATGGCTGT TGGAACTGTT 
GCAGTTGGTG GTTATTGGGC AGGAAGCCGT GATGTTAAAA AAAGGTACAT GAAGCACAAG 
AGAGATGATG GATCAGATAA AAAGCAAGAT GATGAGACTG TGGATGTTAC CCCTATAATG 
ATCTGTGTAT TTGTGGTTAT GTGTTGCTCA ATGCTGATTT TGCTGTATTA CTTTTACGAT 
CACTTAGTGT ATGTGATCAT TGGAATATTT TGCCTTGCTG CATCGATTGG TCTATATAGT 
TGTCTGTCTC CATTTGTCCG CAGATTTCCT TTTGGAAAAT GCAGGGTGCC AGATAATAAC 
CTGCCTTACT TTCACAAGCG ACCGCCTTTT TGGAAGCTAC TGCTGGCTGC TTTCTGTATT 
GTGGTCAGTG TTATCTGGGG AGTATACAGA AATGAAGATC AGTGGGCCTG GGTTCTTCAA 
GATATACTTG GAATAGCATT TTGCCTCTAC ATGCTGAAGA CAATCAGAAT GCCCACATTT 
AAGGGCTGCA CTTTGCTTTT ATTTGTGCTC TTTATTTATG ATGTGTTCTT TGTCTTCATC 
ACTCCGTACC TCACCAAGCG TGGGGAGAGC ATCATGGTGG AAGTTGCCTC TGGGCCATCA 
AATTCTACTA CCCAGGAAAA GCTTCCAATG GTCCTTAAAG TGCCAAGGTT AAATTCCTCG 
CCCCTAGCTC TCTGTGACAG ACCATTCTCT CTCTTGGGCT TTGGGGATAT CTTGGTACCA 
GGACTGCTTG TTGCCTACTG TCACAGGTTT GATATCCAGG TACAATCATC AAGGATTTAC 
TTTGTTGCCT GCACTATAGC CTATGGAATA GGCCTTCTGG TGACTTTTGT AGCACTGGCA 
TTAATGCAAA AGGGACAGCC TGCTCTGCTC TATCTCGTCC CCTGTACCCT TCTTACCAGC 
CTTGCAGTAG CACTCTGGCG CAAGGAATTA CACATGTTCT GGACCGGAAG CGGATTTGTG 
AAAGACCTTC CTCAGCCCCC TTTAGTAGTG GAACCAAGTG GCTCTGATGC ATGCTTAGGA 
AAGAAGACAG AGGATCAAGA GCATGAGGAA GACCCAAACA GATGCCCTGC CGAGCCACAT 
CATTTTTCAG ATCTAGAGAC ATCTAAACTT GATGAGCAAC AGGATAGAGC ATTGTCTCAG 
GCTGACCAGG TTGTTTACCC AGACACAAAT CATCAGAGAT CATCCGATAT GGACATTACG 
GGAACAGTTC CTGAATTGCC AGAGCCCTCA TCAGATGGTG AAATAAACAT TTCAGATACC 
TCTGTATCCA CAGAGTTACA GTCTACCTCT GCAGTACATG GAGAAACACC AACCATGCAA 
AAGAGCCATC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sppl2b ( XM_012966166.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821620.1 Xenopus tropicalis signal peptide peptidase like 2B (sppl2b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa29694
Clone ID Related Accession (Same CDS sequence)	XM_012966166.2 , XM_012966162.2
Accession Version	XM_012966166.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	signal peptide peptidase-like 2B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966166.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTTC TTTTGATTGT CCTGTTATGG GGTTTGTTTG TAACAGAGGT GGTGGGTGAA 
TACGGAATGG TTCATGTCAC ATCGGAAAAG AGCGGCTCCA GTTCCAAAGA TTACTGCATT 
TTGTTTAATT CCCAGTGGGC TCACCTTCCC CAAGACTTGG CCAAAGCTGG CCTCTTGCAA 
CTCCAGAATG AGACTGAATC TGCATTATGT TCCCCTTCAG ATGTGCCAGA AGGGGGATTC 
TTTAACCGCA TCCCAATGGT CATGCGAGGG AACTGCACAT TTTACGAGAA AGTGCGATTG 
GCACAGGTTA ATGGAGCTAA GGGTCTGCTA ATTGTCAGCA GAGAAAGACT GGTACCACCA 
GGGGGTAATC AGAGTCAGTT TGAAGAAATA GATATTCCTG TGGCTCTCCT TAGCTACAGT 
GACATGCTGG ATATTGGCAA AACATTTGGA AAGTCTGTGA AAGTGGCTAT GTATGCACCA 
AATGAACCTG TGCTGGACTA CAACATGGTC ATTATCTTTT TGATGGCTGT TGGAACTGTT 
GCAGTTGGTG GTTATTGGGC AGGAAGCCGT GATGTTAAAA AAAGGTACAT GAAGCACAAG 
AGAGATGATG GATCAGATAA AAAGCAAGAT GATGAGACTG TGGATGTTAC CCCTATAATG 
ATCTGTGTAT TTGTGGTTAT GTGTTGCTCA ATGCTGATTT TGCTGTATTA CTTTTACGAT 
CACTTAGTGT ATGTGATCAT TGGAATATTT TGCCTTGCTG CATCGATTGG TCTATATAGT 
TGTCTGTCTC CATTTGTCCG CAGATTTCCT TTTGGAAAAT GCAGGGTGCC AGATAATAAC 
CTGCCTTACT TTCACAAGCG ACCGCCTTTT TGGAAGCTAC TGCTGGCTGC TTTCTGTATT 
GTGGTCAGTG TTATCTGGGG AGTATACAGA AATGAAGATC AGTGGGCCTG GGTTCTTCAA 
GATATACTTG GAATAGCATT TTGCCTCTAC ATGCTGAAGA CAATCAGAAT GCCCACATTT 
AAGGGCTGCA CTTTGCTTTT ATTTGTGCTC TTTATTTATG ATGTGTTCTT TGTCTTCATC 
ACTCCGTACC TCACCAAGCG TGGGGAGAGC ATCATGGTGG AAGTTGCCTC TGGGCCATCA 
AATTCTACTA CCCAGGAAAA GCTTCCAATG GTCCTTAAAG TGCCAAGGTT AAATTCCTCG 
CCCCTAGCTC TCTGTGACAG ACCATTCTCT CTCTTGGGCT TTGGGGATAT CTTGGTACCA 
GGACTGCTTG TTGCCTACTG TCACAGGTTT GATATCCAGG TACAATCATC AAGGATTTAC 
TTTGTTGCCT GCACTATAGC CTATGGAATA GGCCTTCTGG TGACTTTTGT AGCACTGGCA 
TTAATGCAAA AGGGACAGCC TGCTCTGCTC TATCTCGTCC CCTGTACCCT TCTTACCAGC 
CTTGCAGTAG CACTCTGGCG CAAGGAATTA CACATGTTCT GGACCGGAAG CGGATTTGTG 
GTGAATAACA GTTTGATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821620.1
CDS	215..1735
Translation	MKLLLIVLLWGLFVTEVVGEYGMVHVTSEKSGSSSKDYCILFNSQWAHLPQDLAKAGLLQLQNETESALCSPSDVPEGGFFNRIPMVMRGNCTFYEKVRLAQVNGAKGLLIVSRERLVPPGGNQSQFEEIDIPVALLSYSDMLDIGKTFGKSVKVAMYAPNEPVLDYNMVIIFLMAVGTVAVGGYWAGSRDVKKRYMKHKRDDGSDKKQDDETVDVTPIMICVFVVMCCSMLILLYYFYDHLVYVIIGIFCLAASIGLYSCLSPFVRRFPFGKCRVPDNNLPYFHKRPPFWKLLLAAFCIVVSVIWGVYRNEDQWAWVLQDILGIAFCLYMLKTIRMPTFKGCTLLLFVLFIYDVFFVFITPYLTKRGESIMVEVASGPSNSTTQEKLPMVLKVPRLNSSPLALCDRPFSLLGFGDILVPGLLVAYCHRFDIQVQSSRIYFVACTIAYGIGLLVTFVALALMQKGQPALLYLVPCTLLTSLAVALWRKELHMFWTGSGFVVNNSLI

Target ORF information:

RefSeq Version	XM_012966166.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis signal peptide peptidase like 2B (sppl2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966166.2

ORF Insert Sequence:

ATGAAGCTTC TTTTGATTGT CCTGTTATGG GGTTTGTTTG TAACAGAGGT GGTGGGTGAA 
TACGGAATGG TTCATGTCAC ATCGGAAAAG AGCGGCTCCA GTTCCAAAGA TTACTGCATT 
TTGTTTAATT CCCAGTGGGC TCACCTTCCC CAAGACTTGG CCAAAGCTGG CCTCTTGCAA 
CTCCAGAATG AGACTGAATC TGCATTATGT TCCCCTTCAG ATGTGCCAGA AGGGGGATTC 
TTTAACCGCA TCCCAATGGT CATGCGAGGG AACTGCACAT TTTACGAGAA AGTGCGATTG 
GCACAGGTTA ATGGAGCTAA GGGTCTGCTA ATTGTCAGCA GAGAAAGACT GGTACCACCA 
GGGGGTAATC AGAGTCAGTT TGAAGAAATA GATATTCCTG TGGCTCTCCT TAGCTACAGT 
GACATGCTGG ATATTGGCAA AACATTTGGA AAGTCTGTGA AAGTGGCTAT GTATGCACCA 
AATGAACCTG TGCTGGACTA CAACATGGTC ATTATCTTTT TGATGGCTGT TGGAACTGTT 
GCAGTTGGTG GTTATTGGGC AGGAAGCCGT GATGTTAAAA AAAGGTACAT GAAGCACAAG 
AGAGATGATG GATCAGATAA AAAGCAAGAT GATGAGACTG TGGATGTTAC CCCTATAATG 
ATCTGTGTAT TTGTGGTTAT GTGTTGCTCA ATGCTGATTT TGCTGTATTA CTTTTACGAT 
CACTTAGTGT ATGTGATCAT TGGAATATTT TGCCTTGCTG CATCGATTGG TCTATATAGT 
TGTCTGTCTC CATTTGTCCG CAGATTTCCT TTTGGAAAAT GCAGGGTGCC AGATAATAAC 
CTGCCTTACT TTCACAAGCG ACCGCCTTTT TGGAAGCTAC TGCTGGCTGC TTTCTGTATT 
GTGGTCAGTG TTATCTGGGG AGTATACAGA AATGAAGATC AGTGGGCCTG GGTTCTTCAA 
GATATACTTG GAATAGCATT TTGCCTCTAC ATGCTGAAGA CAATCAGAAT GCCCACATTT 
AAGGGCTGCA CTTTGCTTTT ATTTGTGCTC TTTATTTATG ATGTGTTCTT TGTCTTCATC 
ACTCCGTACC TCACCAAGCG TGGGGAGAGC ATCATGGTGG AAGTTGCCTC TGGGCCATCA 
AATTCTACTA CCCAGGAAAA GCTTCCAATG GTCCTTAAAG TGCCAAGGTT AAATTCCTCG 
CCCCTAGCTC TCTGTGACAG ACCATTCTCT CTCTTGGGCT TTGGGGATAT CTTGGTACCA 
GGACTGCTTG TTGCCTACTG TCACAGGTTT GATATCCAGG TACAATCATC AAGGATTTAC 
TTTGTTGCCT GCACTATAGC CTATGGAATA GGCCTTCTGG TGACTTTTGT AGCACTGGCA 
TTAATGCAAA AGGGACAGCC TGCTCTGCTC TATCTCGTCC CCTGTACCCT TCTTACCAGC 
CTTGCAGTAG CACTCTGGCG CAAGGAATTA CACATGTTCT GGACCGGAAG CGGATTTGTG 
GTGAATAACA GTTTGATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sppl2b ( XM_012966162.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821616.1 Xenopus tropicalis signal peptide peptidase like 2B (sppl2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa29694
Clone ID Related Accession (Same CDS sequence)	XM_012966166.2 , XM_012966162.2
Accession Version	XM_012966162.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	signal peptide peptidase-like 2B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966162.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTTC TTTTGATTGT CCTGTTATGG GGTTTGTTTG TAACAGAGGT GGTGGGTGAA 
TACGGAATGG TTCATGTCAC ATCGGAAAAG AGCGGCTCCA GTTCCAAAGA TTACTGCATT 
TTGTTTAATT CCCAGTGGGC TCACCTTCCC CAAGACTTGG CCAAAGCTGG CCTCTTGCAA 
CTCCAGAATG AGACTGAATC TGCATTATGT TCCCCTTCAG ATGTGCCAGA AGGGGGATTC 
TTTAACCGCA TCCCAATGGT CATGCGAGGG AACTGCACAT TTTACGAGAA AGTGCGATTG 
GCACAGGTTA ATGGAGCTAA GGGTCTGCTA ATTGTCAGCA GAGAAAGACT GGTACCACCA 
GGGGGTAATC AGAGTCAGTT TGAAGAAATA GATATTCCTG TGGCTCTCCT TAGCTACAGT 
GACATGCTGG ATATTGGCAA AACATTTGGA AAGTCTGTGA AAGTGGCTAT GTATGCACCA 
AATGAACCTG TGCTGGACTA CAACATGGTC ATTATCTTTT TGATGGCTGT TGGAACTGTT 
GCAGTTGGTG GTTATTGGGC AGGAAGCCGT GATGTTAAAA AAAGGTACAT GAAGCACAAG 
AGAGATGATG GATCAGATAA AAAGCAAGAT GATGAGACTG TGGATGTTAC CCCTATAATG 
ATCTGTGTAT TTGTGGTTAT GTGTTGCTCA ATGCTGATTT TGCTGTATTA CTTTTACGAT 
CACTTAGTGT ATGTGATCAT TGGAATATTT TGCCTTGCTG CATCGATTGG TCTATATAGT 
TGTCTGTCTC CATTTGTCCG CAGATTTCCT TTTGGAAAAT GCAGGGTGCC AGATAATAAC 
CTGCCTTACT TTCACAAGCG ACCGCCTTTT TGGAAGCTAC TGCTGGCTGC TTTCTGTATT 
GTGGTCAGTG TTATCTGGGG AGTATACAGA AATGAAGATC AGTGGGCCTG GGTTCTTCAA 
GATATACTTG GAATAGCATT TTGCCTCTAC ATGCTGAAGA CAATCAGAAT GCCCACATTT 
AAGGGCTGCA CTTTGCTTTT ATTTGTGCTC TTTATTTATG ATGTGTTCTT TGTCTTCATC 
ACTCCGTACC TCACCAAGCG TGGGGAGAGC ATCATGGTGG AAGTTGCCTC TGGGCCATCA 
AATTCTACTA CCCAGGAAAA GCTTCCAATG GTCCTTAAAG TGCCAAGGTT AAATTCCTCG 
CCCCTAGCTC TCTGTGACAG ACCATTCTCT CTCTTGGGCT TTGGGGATAT CTTGGTACCA 
GGACTGCTTG TTGCCTACTG TCACAGGTTT GATATCCAGG TACAATCATC AAGGATTTAC 
TTTGTTGCCT GCACTATAGC CTATGGAATA GGCCTTCTGG TGACTTTTGT AGCACTGGCA 
TTAATGCAAA AGGGACAGCC TGCTCTGCTC TATCTCGTCC CCTGTACCCT TCTTACCAGC 
CTTGCAGTAG CACTCTGGCG CAAGGAATTA CACATGTTCT GGACCGGAAG CGGATTTGTG 
GTGAATAACA GTTTGATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821616.1
CDS	215..1735
Translation	MKLLLIVLLWGLFVTEVVGEYGMVHVTSEKSGSSSKDYCILFNSQWAHLPQDLAKAGLLQLQNETESALCSPSDVPEGGFFNRIPMVMRGNCTFYEKVRLAQVNGAKGLLIVSRERLVPPGGNQSQFEEIDIPVALLSYSDMLDIGKTFGKSVKVAMYAPNEPVLDYNMVIIFLMAVGTVAVGGYWAGSRDVKKRYMKHKRDDGSDKKQDDETVDVTPIMICVFVVMCCSMLILLYYFYDHLVYVIIGIFCLAASIGLYSCLSPFVRRFPFGKCRVPDNNLPYFHKRPPFWKLLLAAFCIVVSVIWGVYRNEDQWAWVLQDILGIAFCLYMLKTIRMPTFKGCTLLLFVLFIYDVFFVFITPYLTKRGESIMVEVASGPSNSTTQEKLPMVLKVPRLNSSPLALCDRPFSLLGFGDILVPGLLVAYCHRFDIQVQSSRIYFVACTIAYGIGLLVTFVALALMQKGQPALLYLVPCTLLTSLAVALWRKELHMFWTGSGFVVNNSLI

Target ORF information:

RefSeq Version	XM_012966162.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis signal peptide peptidase like 2B (sppl2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966162.2

ORF Insert Sequence:

ATGAAGCTTC TTTTGATTGT CCTGTTATGG GGTTTGTTTG TAACAGAGGT GGTGGGTGAA 
TACGGAATGG TTCATGTCAC ATCGGAAAAG AGCGGCTCCA GTTCCAAAGA TTACTGCATT 
TTGTTTAATT CCCAGTGGGC TCACCTTCCC CAAGACTTGG CCAAAGCTGG CCTCTTGCAA 
CTCCAGAATG AGACTGAATC TGCATTATGT TCCCCTTCAG ATGTGCCAGA AGGGGGATTC 
TTTAACCGCA TCCCAATGGT CATGCGAGGG AACTGCACAT TTTACGAGAA AGTGCGATTG 
GCACAGGTTA ATGGAGCTAA GGGTCTGCTA ATTGTCAGCA GAGAAAGACT GGTACCACCA 
GGGGGTAATC AGAGTCAGTT TGAAGAAATA GATATTCCTG TGGCTCTCCT TAGCTACAGT 
GACATGCTGG ATATTGGCAA AACATTTGGA AAGTCTGTGA AAGTGGCTAT GTATGCACCA 
AATGAACCTG TGCTGGACTA CAACATGGTC ATTATCTTTT TGATGGCTGT TGGAACTGTT 
GCAGTTGGTG GTTATTGGGC AGGAAGCCGT GATGTTAAAA AAAGGTACAT GAAGCACAAG 
AGAGATGATG GATCAGATAA AAAGCAAGAT GATGAGACTG TGGATGTTAC CCCTATAATG 
ATCTGTGTAT TTGTGGTTAT GTGTTGCTCA ATGCTGATTT TGCTGTATTA CTTTTACGAT 
CACTTAGTGT ATGTGATCAT TGGAATATTT TGCCTTGCTG CATCGATTGG TCTATATAGT 
TGTCTGTCTC CATTTGTCCG CAGATTTCCT TTTGGAAAAT GCAGGGTGCC AGATAATAAC 
CTGCCTTACT TTCACAAGCG ACCGCCTTTT TGGAAGCTAC TGCTGGCTGC TTTCTGTATT 
GTGGTCAGTG TTATCTGGGG AGTATACAGA AATGAAGATC AGTGGGCCTG GGTTCTTCAA 
GATATACTTG GAATAGCATT TTGCCTCTAC ATGCTGAAGA CAATCAGAAT GCCCACATTT 
AAGGGCTGCA CTTTGCTTTT ATTTGTGCTC TTTATTTATG ATGTGTTCTT TGTCTTCATC 
ACTCCGTACC TCACCAAGCG TGGGGAGAGC ATCATGGTGG AAGTTGCCTC TGGGCCATCA 
AATTCTACTA CCCAGGAAAA GCTTCCAATG GTCCTTAAAG TGCCAAGGTT AAATTCCTCG 
CCCCTAGCTC TCTGTGACAG ACCATTCTCT CTCTTGGGCT TTGGGGATAT CTTGGTACCA 
GGACTGCTTG TTGCCTACTG TCACAGGTTT GATATCCAGG TACAATCATC AAGGATTTAC 
TTTGTTGCCT GCACTATAGC CTATGGAATA GGCCTTCTGG TGACTTTTGT AGCACTGGCA 
TTAATGCAAA AGGGACAGCC TGCTCTGCTC TATCTCGTCC CCTGTACCCT TCTTACCAGC 
CTTGCAGTAG CACTCTGGCG CAAGGAATTA CACATGTTCT GGACCGGAAG CGGATTTGTG 
GTGAATAACA GTTTGATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sppl2b ( XM_012966156.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821610.2 Xenopus tropicalis signal peptide peptidase like 2B (sppl2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa48079
Clone ID Related Accession (Same CDS sequence)	XM_012966156.3
Accession Version	XM_012966156.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	signal peptide peptidase-like 2B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966156.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTTC TTTTGATTTT CCTGTTATGG GGTTTGTTTG TAACAGAGGT GGTGGGTGAA 
TACGGAATGG TTCATGTCAC ATCGGAAAAG AGCGGCTCCA GTTCCAAAGA TTACTGCATT 
TTGTTTAATT CCCAGTGGGC TCACCTTCCC CAAGACTTGG CCAAAGCTGG CCTCTTGCAA 
CTCCAGAATG AGACTGAATC TGCATTATGT TCCCCTTCAG ATGTGCCAGA AGGGGGATTC 
TTTAACCGCA TCCCAATGGT CATGCGAGGG AACTGCACAT TTTACGAGAA AGTGCGATTG 
GCACAGGTTA ATGGAGCTAA GGGTCTGCTA ATTGTCAGCA GAGAAAGACT GGTACCACCA 
GGGGGTAATC AGAGTCAGTT TGAAGAAATA GATATTCCTG TGGCTCTCCT TAGCTACAGT 
GACATGCTGG ATATTGGCAA AACATTTGGA AAGTCTGTGA AAGTGGCTAT GTATGCACCA 
AATGAACCTG TGCTGGACTA CAACATGGTC ATTATCTTTT TGATGGCTGT TGGAACTGTT 
GCAGTTGGTG GTTATTGGGC AGGAAGCCGT GATGTTAAAA AAAGGTACAT GAAGCACAAG 
AGAGATGATG GATCAGATAA AAAGCAAGAT GATGAGACTG TGGATGTTAC CCCTATAATG 
ATCTGTGTAT TTGTGGTTAT GTGTTGCTCA ATGCTGATTT TGCTGTATTA CTTTTACGAT 
CACTTAGTGT ATGTGATCAT TGGAATATTT TGCCTTGCTG CATCGATTGG TCTATATAGT 
TGTCTGTCTC CATTTGTCCG CAGATTTCCT TTTGGAAAAT GCAGGGTGCC AGATAATAAC 
CTGCCTTACT TTCACAAGCG ACCGCCTTTT TGGAAGCTAC TGCTGGCTGC TTTCTGTATT 
GTGGTCAGTG TTATCTGGGG AGTATACAGA AATGAAGATC AGTGGGCCTG GGTTCTTCAA 
GATATACTTG GAATAGCATT TTGCCTCTAC ATGCTGAAGA CAATCAGAAT GCCCACATTT 
AAGGGCTGCA CTTTGCTTTT ATTTGTGCTC TTTATTTATG ATGTGTTCTT TGTCTTCATC 
ACTCCGTACC TCACCAAGCG TGGGGAGAGC ATCATGGTGG AAGTTGCCTC TGGGCCATCA 
AATTCTACTA CCCAGGAAAA GCTTCCAATG GTCCTTAAAG TGCCAAGGTT AAATTCCTCG 
CCCCTAGCTC TCTGTGACAG ACCATTCTCT CTCTTGGGCT TTGGGGATAT CTTGGTACCA 
GGACTGCTTG TTGCCTACTG TCACAGGTTT GATATCCAGG TACAATCATC AAGGATTTAC 
TTTGTTGCCT GCACTATAGC CTATGGAATA GGCCTTCTGG TGACTTTTGT AGCACTGGCA 
TTAATGCAAA AGGGACAGCC TGCTCTGCTC TATCTCGTCC CCTGTACCCT TCTTACCAGC 
CTTGCAGTAG CACTCTGGCG CAAGGAATTA CACATGTTCT GGACCGGAAG CGGATTTGTG 
AAAGACCTTC CTCAGCCCCC TTTAGTAGTG GAACCAAGTG GCTCTGATGC ATGCTTAGGA 
AAGAAGACAG AGGATCAAGA GCATGAGGAA GACCCAAACA GATGCCCTGC CGAGCCACAT 
CATTTTTCAG ATCTAGAGAC ATCTAAACTT GATGAGCAAC AGGATAGAGC ATTGTCTCAG 
GCTGACCAGG TTGTTTACCC AGACACAAAT CATCAGAGAT CATCCGATAT GGACATTACG 
GGAACAGTTC CTGAATTGCC AGAGCCCTCA TCAGATGGTG AAATAAACAT TTCAGATACC 
TCTGTATCCA CAGAGTTACA GTCTACCTCT GCAGTACATG GAGAAACACC AACCATGCAA 
AAGAGCCATC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821610.2
CDS	216..2090
Translation	MKLLLIFLLWGLFVTEVVGEYGMVHVTSEKSGSSSKDYCILFNSQWAHLPQDLAKAGLLQLQNETESALCSPSDVPEGGFFNRIPMVMRGNCTFYEKVRLAQVNGAKGLLIVSRERLVPPGGNQSQFEEIDIPVALLSYSDMLDIGKTFGKSVKVAMYAPNEPVLDYNMVIIFLMAVGTVAVGGYWAGSRDVKKRYMKHKRDDGSDKKQDDETVDVTPIMICVFVVMCCSMLILLYYFYDHLVYVIIGIFCLAASIGLYSCLSPFVRRFPFGKCRVPDNNLPYFHKRPPFWKLLLAAFCIVVSVIWGVYRNEDQWAWVLQDILGIAFCLYMLKTIRMPTFKGCTLLLFVLFIYDVFFVFITPYLTKRGESIMVEVASGPSNSTTQEKLPMVLKVPRLNSSPLALCDRPFSLLGFGDILVPGLLVAYCHRFDIQVQSSRIYFVACTIAYGIGLLVTFVALALMQKGQPALLYLVPCTLLTSLAVALWRKELHMFWTGSGFVKDLPQPPLVVEPSGSDACLGKKTEDQEHEEDPNRCPAEPHHFSDLETSKLDEQQDRALSQADQVVYPDTNHQRSSDMDITGTVPELPEPSSDGEINISDTSVSTELQSTSAVHGETPTMQKSHQ

Target ORF information:

RefSeq Version	XM_012966156.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis signal peptide peptidase like 2B (sppl2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966156.3

ORF Insert Sequence:

ATGAAGCTTC TTTTGATTTT CCTGTTATGG GGTTTGTTTG TAACAGAGGT GGTGGGTGAA 
TACGGAATGG TTCATGTCAC ATCGGAAAAG AGCGGCTCCA GTTCCAAAGA TTACTGCATT 
TTGTTTAATT CCCAGTGGGC TCACCTTCCC CAAGACTTGG CCAAAGCTGG CCTCTTGCAA 
CTCCAGAATG AGACTGAATC TGCATTATGT TCCCCTTCAG ATGTGCCAGA AGGGGGATTC 
TTTAACCGCA TCCCAATGGT CATGCGAGGG AACTGCACAT TTTACGAGAA AGTGCGATTG 
GCACAGGTTA ATGGAGCTAA GGGTCTGCTA ATTGTCAGCA GAGAAAGACT GGTACCACCA 
GGGGGTAATC AGAGTCAGTT TGAAGAAATA GATATTCCTG TGGCTCTCCT TAGCTACAGT 
GACATGCTGG ATATTGGCAA AACATTTGGA AAGTCTGTGA AAGTGGCTAT GTATGCACCA 
AATGAACCTG TGCTGGACTA CAACATGGTC ATTATCTTTT TGATGGCTGT TGGAACTGTT 
GCAGTTGGTG GTTATTGGGC AGGAAGCCGT GATGTTAAAA AAAGGTACAT GAAGCACAAG 
AGAGATGATG GATCAGATAA AAAGCAAGAT GATGAGACTG TGGATGTTAC CCCTATAATG 
ATCTGTGTAT TTGTGGTTAT GTGTTGCTCA ATGCTGATTT TGCTGTATTA CTTTTACGAT 
CACTTAGTGT ATGTGATCAT TGGAATATTT TGCCTTGCTG CATCGATTGG TCTATATAGT 
TGTCTGTCTC CATTTGTCCG CAGATTTCCT TTTGGAAAAT GCAGGGTGCC AGATAATAAC 
CTGCCTTACT TTCACAAGCG ACCGCCTTTT TGGAAGCTAC TGCTGGCTGC TTTCTGTATT 
GTGGTCAGTG TTATCTGGGG AGTATACAGA AATGAAGATC AGTGGGCCTG GGTTCTTCAA 
GATATACTTG GAATAGCATT TTGCCTCTAC ATGCTGAAGA CAATCAGAAT GCCCACATTT 
AAGGGCTGCA CTTTGCTTTT ATTTGTGCTC TTTATTTATG ATGTGTTCTT TGTCTTCATC 
ACTCCGTACC TCACCAAGCG TGGGGAGAGC ATCATGGTGG AAGTTGCCTC TGGGCCATCA 
AATTCTACTA CCCAGGAAAA GCTTCCAATG GTCCTTAAAG TGCCAAGGTT AAATTCCTCG 
CCCCTAGCTC TCTGTGACAG ACCATTCTCT CTCTTGGGCT TTGGGGATAT CTTGGTACCA 
GGACTGCTTG TTGCCTACTG TCACAGGTTT GATATCCAGG TACAATCATC AAGGATTTAC 
TTTGTTGCCT GCACTATAGC CTATGGAATA GGCCTTCTGG TGACTTTTGT AGCACTGGCA 
TTAATGCAAA AGGGACAGCC TGCTCTGCTC TATCTCGTCC CCTGTACCCT TCTTACCAGC 
CTTGCAGTAG CACTCTGGCG CAAGGAATTA CACATGTTCT GGACCGGAAG CGGATTTGTG 
AAAGACCTTC CTCAGCCCCC TTTAGTAGTG GAACCAAGTG GCTCTGATGC ATGCTTAGGA 
AAGAAGACAG AGGATCAAGA GCATGAGGAA GACCCAAACA GATGCCCTGC CGAGCCACAT 
CATTTTTCAG ATCTAGAGAC ATCTAAACTT GATGAGCAAC AGGATAGAGC ATTGTCTCAG 
GCTGACCAGG TTGTTTACCC AGACACAAAT CATCAGAGAT CATCCGATAT GGACATTACG 
GGAACAGTTC CTGAATTGCC AGAGCCCTCA TCAGATGGTG AAATAAACAT TTCAGATACC 
TCTGTATCCA CAGAGTTACA GTCTACCTCT GCAGTACATG GAGAAACACC AACCATGCAA 
AAGAGCCATC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sppl2b ( XM_012966162.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821616.2 Xenopus tropicalis signal peptide peptidase like 2B (sppl2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa48080
Clone ID Related Accession (Same CDS sequence)	XM_012966162.3 , XM_012966166.3
Accession Version	XM_012966162.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	signal peptide peptidase-like 2B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966162.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTTC TTTTGATTTT CCTGTTATGG GGTTTGTTTG TAACAGAGGT GGTGGGTGAA 
TACGGAATGG TTCATGTCAC ATCGGAAAAG AGCGGCTCCA GTTCCAAAGA TTACTGCATT 
TTGTTTAATT CCCAGTGGGC TCACCTTCCC CAAGACTTGG CCAAAGCTGG CCTCTTGCAA 
CTCCAGAATG AGACTGAATC TGCATTATGT TCCCCTTCAG ATGTGCCAGA AGGGGGATTC 
TTTAACCGCA TCCCAATGGT CATGCGAGGG AACTGCACAT TTTACGAGAA AGTGCGATTG 
GCACAGGTTA ATGGAGCTAA GGGTCTGCTA ATTGTCAGCA GAGAAAGACT GGTACCACCA 
GGGGGTAATC AGAGTCAGTT TGAAGAAATA GATATTCCTG TGGCTCTCCT TAGCTACAGT 
GACATGCTGG ATATTGGCAA AACATTTGGA AAGTCTGTGA AAGTGGCTAT GTATGCACCA 
AATGAACCTG TGCTGGACTA CAACATGGTC ATTATCTTTT TGATGGCTGT TGGAACTGTT 
GCAGTTGGTG GTTATTGGGC AGGAAGCCGT GATGTTAAAA AAAGGTACAT GAAGCACAAG 
AGAGATGATG GATCAGATAA AAAGCAAGAT GATGAGACTG TGGATGTTAC CCCTATAATG 
ATCTGTGTAT TTGTGGTTAT GTGTTGCTCA ATGCTGATTT TGCTGTATTA CTTTTACGAT 
CACTTAGTGT ATGTGATCAT TGGAATATTT TGCCTTGCTG CATCGATTGG TCTATATAGT 
TGTCTGTCTC CATTTGTCCG CAGATTTCCT TTTGGAAAAT GCAGGGTGCC AGATAATAAC 
CTGCCTTACT TTCACAAGCG ACCGCCTTTT TGGAAGCTAC TGCTGGCTGC TTTCTGTATT 
GTGGTCAGTG TTATCTGGGG AGTATACAGA AATGAAGATC AGTGGGCCTG GGTTCTTCAA 
GATATACTTG GAATAGCATT TTGCCTCTAC ATGCTGAAGA CAATCAGAAT GCCCACATTT 
AAGGGCTGCA CTTTGCTTTT ATTTGTGCTC TTTATTTATG ATGTGTTCTT TGTCTTCATC 
ACTCCGTACC TCACCAAGCG TGGGGAGAGC ATCATGGTGG AAGTTGCCTC TGGGCCATCA 
AATTCTACTA CCCAGGAAAA GCTTCCAATG GTCCTTAAAG TGCCAAGGTT AAATTCCTCG 
CCCCTAGCTC TCTGTGACAG ACCATTCTCT CTCTTGGGCT TTGGGGATAT CTTGGTACCA 
GGACTGCTTG TTGCCTACTG TCACAGGTTT GATATCCAGG TACAATCATC AAGGATTTAC 
TTTGTTGCCT GCACTATAGC CTATGGAATA GGCCTTCTGG TGACTTTTGT AGCACTGGCA 
TTAATGCAAA AGGGACAGCC TGCTCTGCTC TATCTCGTCC CCTGTACCCT TCTTACCAGC 
CTTGCAGTAG CACTCTGGCG CAAGGAATTA CACATGTTCT GGACCGGAAG CGGATTTGTG 
GTGAATAACA GTTTGATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821616.2
CDS	216..1736
Translation	MKLLLIFLLWGLFVTEVVGEYGMVHVTSEKSGSSSKDYCILFNSQWAHLPQDLAKAGLLQLQNETESALCSPSDVPEGGFFNRIPMVMRGNCTFYEKVRLAQVNGAKGLLIVSRERLVPPGGNQSQFEEIDIPVALLSYSDMLDIGKTFGKSVKVAMYAPNEPVLDYNMVIIFLMAVGTVAVGGYWAGSRDVKKRYMKHKRDDGSDKKQDDETVDVTPIMICVFVVMCCSMLILLYYFYDHLVYVIIGIFCLAASIGLYSCLSPFVRRFPFGKCRVPDNNLPYFHKRPPFWKLLLAAFCIVVSVIWGVYRNEDQWAWVLQDILGIAFCLYMLKTIRMPTFKGCTLLLFVLFIYDVFFVFITPYLTKRGESIMVEVASGPSNSTTQEKLPMVLKVPRLNSSPLALCDRPFSLLGFGDILVPGLLVAYCHRFDIQVQSSRIYFVACTIAYGIGLLVTFVALALMQKGQPALLYLVPCTLLTSLAVALWRKELHMFWTGSGFVVNNSLI

Target ORF information:

RefSeq Version	XM_012966162.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis signal peptide peptidase like 2B (sppl2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966162.3

ORF Insert Sequence:

ATGAAGCTTC TTTTGATTTT CCTGTTATGG GGTTTGTTTG TAACAGAGGT GGTGGGTGAA 
TACGGAATGG TTCATGTCAC ATCGGAAAAG AGCGGCTCCA GTTCCAAAGA TTACTGCATT 
TTGTTTAATT CCCAGTGGGC TCACCTTCCC CAAGACTTGG CCAAAGCTGG CCTCTTGCAA 
CTCCAGAATG AGACTGAATC TGCATTATGT TCCCCTTCAG ATGTGCCAGA AGGGGGATTC 
TTTAACCGCA TCCCAATGGT CATGCGAGGG AACTGCACAT TTTACGAGAA AGTGCGATTG 
GCACAGGTTA ATGGAGCTAA GGGTCTGCTA ATTGTCAGCA GAGAAAGACT GGTACCACCA 
GGGGGTAATC AGAGTCAGTT TGAAGAAATA GATATTCCTG TGGCTCTCCT TAGCTACAGT 
GACATGCTGG ATATTGGCAA AACATTTGGA AAGTCTGTGA AAGTGGCTAT GTATGCACCA 
AATGAACCTG TGCTGGACTA CAACATGGTC ATTATCTTTT TGATGGCTGT TGGAACTGTT 
GCAGTTGGTG GTTATTGGGC AGGAAGCCGT GATGTTAAAA AAAGGTACAT GAAGCACAAG 
AGAGATGATG GATCAGATAA AAAGCAAGAT GATGAGACTG TGGATGTTAC CCCTATAATG 
ATCTGTGTAT TTGTGGTTAT GTGTTGCTCA ATGCTGATTT TGCTGTATTA CTTTTACGAT 
CACTTAGTGT ATGTGATCAT TGGAATATTT TGCCTTGCTG CATCGATTGG TCTATATAGT 
TGTCTGTCTC CATTTGTCCG CAGATTTCCT TTTGGAAAAT GCAGGGTGCC AGATAATAAC 
CTGCCTTACT TTCACAAGCG ACCGCCTTTT TGGAAGCTAC TGCTGGCTGC TTTCTGTATT 
GTGGTCAGTG TTATCTGGGG AGTATACAGA AATGAAGATC AGTGGGCCTG GGTTCTTCAA 
GATATACTTG GAATAGCATT TTGCCTCTAC ATGCTGAAGA CAATCAGAAT GCCCACATTT 
AAGGGCTGCA CTTTGCTTTT ATTTGTGCTC TTTATTTATG ATGTGTTCTT TGTCTTCATC 
ACTCCGTACC TCACCAAGCG TGGGGAGAGC ATCATGGTGG AAGTTGCCTC TGGGCCATCA 
AATTCTACTA CCCAGGAAAA GCTTCCAATG GTCCTTAAAG TGCCAAGGTT AAATTCCTCG 
CCCCTAGCTC TCTGTGACAG ACCATTCTCT CTCTTGGGCT TTGGGGATAT CTTGGTACCA 
GGACTGCTTG TTGCCTACTG TCACAGGTTT GATATCCAGG TACAATCATC AAGGATTTAC 
TTTGTTGCCT GCACTATAGC CTATGGAATA GGCCTTCTGG TGACTTTTGT AGCACTGGCA 
TTAATGCAAA AGGGACAGCC TGCTCTGCTC TATCTCGTCC CCTGTACCCT TCTTACCAGC 
CTTGCAGTAG CACTCTGGCG CAAGGAATTA CACATGTTCT GGACCGGAAG CGGATTTGTG 
GTGAATAACA GTTTGATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sppl2b ( XM_012966166.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821620.2 Xenopus tropicalis signal peptide peptidase like 2B (sppl2b), transcript variant X3, mRNA.

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CloneID	OXa48080
Clone ID Related Accession (Same CDS sequence)	XM_012966162.3 , XM_012966166.3
Accession Version	XM_012966166.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	signal peptide peptidase-like 2B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966166.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTTC TTTTGATTTT CCTGTTATGG GGTTTGTTTG TAACAGAGGT GGTGGGTGAA 
TACGGAATGG TTCATGTCAC ATCGGAAAAG AGCGGCTCCA GTTCCAAAGA TTACTGCATT 
TTGTTTAATT CCCAGTGGGC TCACCTTCCC CAAGACTTGG CCAAAGCTGG CCTCTTGCAA 
CTCCAGAATG AGACTGAATC TGCATTATGT TCCCCTTCAG ATGTGCCAGA AGGGGGATTC 
TTTAACCGCA TCCCAATGGT CATGCGAGGG AACTGCACAT TTTACGAGAA AGTGCGATTG 
GCACAGGTTA ATGGAGCTAA GGGTCTGCTA ATTGTCAGCA GAGAAAGACT GGTACCACCA 
GGGGGTAATC AGAGTCAGTT TGAAGAAATA GATATTCCTG TGGCTCTCCT TAGCTACAGT 
GACATGCTGG ATATTGGCAA AACATTTGGA AAGTCTGTGA AAGTGGCTAT GTATGCACCA 
AATGAACCTG TGCTGGACTA CAACATGGTC ATTATCTTTT TGATGGCTGT TGGAACTGTT 
GCAGTTGGTG GTTATTGGGC AGGAAGCCGT GATGTTAAAA AAAGGTACAT GAAGCACAAG 
AGAGATGATG GATCAGATAA AAAGCAAGAT GATGAGACTG TGGATGTTAC CCCTATAATG 
ATCTGTGTAT TTGTGGTTAT GTGTTGCTCA ATGCTGATTT TGCTGTATTA CTTTTACGAT 
CACTTAGTGT ATGTGATCAT TGGAATATTT TGCCTTGCTG CATCGATTGG TCTATATAGT 
TGTCTGTCTC CATTTGTCCG CAGATTTCCT TTTGGAAAAT GCAGGGTGCC AGATAATAAC 
CTGCCTTACT TTCACAAGCG ACCGCCTTTT TGGAAGCTAC TGCTGGCTGC TTTCTGTATT 
GTGGTCAGTG TTATCTGGGG AGTATACAGA AATGAAGATC AGTGGGCCTG GGTTCTTCAA 
GATATACTTG GAATAGCATT TTGCCTCTAC ATGCTGAAGA CAATCAGAAT GCCCACATTT 
AAGGGCTGCA CTTTGCTTTT ATTTGTGCTC TTTATTTATG ATGTGTTCTT TGTCTTCATC 
ACTCCGTACC TCACCAAGCG TGGGGAGAGC ATCATGGTGG AAGTTGCCTC TGGGCCATCA 
AATTCTACTA CCCAGGAAAA GCTTCCAATG GTCCTTAAAG TGCCAAGGTT AAATTCCTCG 
CCCCTAGCTC TCTGTGACAG ACCATTCTCT CTCTTGGGCT TTGGGGATAT CTTGGTACCA 
GGACTGCTTG TTGCCTACTG TCACAGGTTT GATATCCAGG TACAATCATC AAGGATTTAC 
TTTGTTGCCT GCACTATAGC CTATGGAATA GGCCTTCTGG TGACTTTTGT AGCACTGGCA 
TTAATGCAAA AGGGACAGCC TGCTCTGCTC TATCTCGTCC CCTGTACCCT TCTTACCAGC 
CTTGCAGTAG CACTCTGGCG CAAGGAATTA CACATGTTCT GGACCGGAAG CGGATTTGTG 
GTGAATAACA GTTTGATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821620.2
CDS	216..1736
Translation	MKLLLIFLLWGLFVTEVVGEYGMVHVTSEKSGSSSKDYCILFNSQWAHLPQDLAKAGLLQLQNETESALCSPSDVPEGGFFNRIPMVMRGNCTFYEKVRLAQVNGAKGLLIVSRERLVPPGGNQSQFEEIDIPVALLSYSDMLDIGKTFGKSVKVAMYAPNEPVLDYNMVIIFLMAVGTVAVGGYWAGSRDVKKRYMKHKRDDGSDKKQDDETVDVTPIMICVFVVMCCSMLILLYYFYDHLVYVIIGIFCLAASIGLYSCLSPFVRRFPFGKCRVPDNNLPYFHKRPPFWKLLLAAFCIVVSVIWGVYRNEDQWAWVLQDILGIAFCLYMLKTIRMPTFKGCTLLLFVLFIYDVFFVFITPYLTKRGESIMVEVASGPSNSTTQEKLPMVLKVPRLNSSPLALCDRPFSLLGFGDILVPGLLVAYCHRFDIQVQSSRIYFVACTIAYGIGLLVTFVALALMQKGQPALLYLVPCTLLTSLAVALWRKELHMFWTGSGFVVNNSLI

Target ORF information:

RefSeq Version	XM_012966166.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis signal peptide peptidase like 2B (sppl2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966166.3

ORF Insert Sequence:

ATGAAGCTTC TTTTGATTTT CCTGTTATGG GGTTTGTTTG TAACAGAGGT GGTGGGTGAA 
TACGGAATGG TTCATGTCAC ATCGGAAAAG AGCGGCTCCA GTTCCAAAGA TTACTGCATT 
TTGTTTAATT CCCAGTGGGC TCACCTTCCC CAAGACTTGG CCAAAGCTGG CCTCTTGCAA 
CTCCAGAATG AGACTGAATC TGCATTATGT TCCCCTTCAG ATGTGCCAGA AGGGGGATTC 
TTTAACCGCA TCCCAATGGT CATGCGAGGG AACTGCACAT TTTACGAGAA AGTGCGATTG 
GCACAGGTTA ATGGAGCTAA GGGTCTGCTA ATTGTCAGCA GAGAAAGACT GGTACCACCA 
GGGGGTAATC AGAGTCAGTT TGAAGAAATA GATATTCCTG TGGCTCTCCT TAGCTACAGT 
GACATGCTGG ATATTGGCAA AACATTTGGA AAGTCTGTGA AAGTGGCTAT GTATGCACCA 
AATGAACCTG TGCTGGACTA CAACATGGTC ATTATCTTTT TGATGGCTGT TGGAACTGTT 
GCAGTTGGTG GTTATTGGGC AGGAAGCCGT GATGTTAAAA AAAGGTACAT GAAGCACAAG 
AGAGATGATG GATCAGATAA AAAGCAAGAT GATGAGACTG TGGATGTTAC CCCTATAATG 
ATCTGTGTAT TTGTGGTTAT GTGTTGCTCA ATGCTGATTT TGCTGTATTA CTTTTACGAT 
CACTTAGTGT ATGTGATCAT TGGAATATTT TGCCTTGCTG CATCGATTGG TCTATATAGT 
TGTCTGTCTC CATTTGTCCG CAGATTTCCT TTTGGAAAAT GCAGGGTGCC AGATAATAAC 
CTGCCTTACT TTCACAAGCG ACCGCCTTTT TGGAAGCTAC TGCTGGCTGC TTTCTGTATT 
GTGGTCAGTG TTATCTGGGG AGTATACAGA AATGAAGATC AGTGGGCCTG GGTTCTTCAA 
GATATACTTG GAATAGCATT TTGCCTCTAC ATGCTGAAGA CAATCAGAAT GCCCACATTT 
AAGGGCTGCA CTTTGCTTTT ATTTGTGCTC TTTATTTATG ATGTGTTCTT TGTCTTCATC 
ACTCCGTACC TCACCAAGCG TGGGGAGAGC ATCATGGTGG AAGTTGCCTC TGGGCCATCA 
AATTCTACTA CCCAGGAAAA GCTTCCAATG GTCCTTAAAG TGCCAAGGTT AAATTCCTCG 
CCCCTAGCTC TCTGTGACAG ACCATTCTCT CTCTTGGGCT TTGGGGATAT CTTGGTACCA 
GGACTGCTTG TTGCCTACTG TCACAGGTTT GATATCCAGG TACAATCATC AAGGATTTAC 
TTTGTTGCCT GCACTATAGC CTATGGAATA GGCCTTCTGG TGACTTTTGT AGCACTGGCA 
TTAATGCAAA AGGGACAGCC TGCTCTGCTC TATCTCGTCC CCTGTACCCT TCTTACCAGC 
CTTGCAGTAG CACTCTGGCG CAAGGAATTA CACATGTTCT GGACCGGAAG CGGATTTGTG 
GTGAATAACA GTTTGATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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sppl2b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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