pde4b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	pde4b
Entrez Gene ID	780324
Full Name	phosphodiesterase 4B
Synonyms	dpde4,pde4b5,pdeivb
General protein information	Preferred Names phosphodiesterase 4B Names cAMP-specific 3',5'-cyclic phosphodiesterase 4B phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031900282.1	XP_031756142.1	cAMP-specific 3',5'-cyclic phosphodiesterase 4B isoform X2
XM_012961423.3	XP_012816877.1	cAMP-specific 3',5'-cyclic phosphodiesterase 4B isoform X4
XM_012961422.1	XP_012816876.1	cAMP-specific 3',5'-cyclic phosphodiesterase 4B isoform X1
XM_012961423.2	XP_012816877.1	cAMP-specific 3',5'-cyclic phosphodiesterase 4B isoform X2
NM_001079395.1	NP_001072863.1	cAMP-specific 3',5'-cyclic phosphodiesterase 4B
XM_031900281.1	XP_031756141.1	cAMP-specific 3',5'-cyclic phosphodiesterase 4B isoform X1
XM_012961422.2	XP_012816876.1	cAMP-specific 3',5'-cyclic phosphodiesterase 4B isoform X3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pde4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pde4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa25098	NM_001079395.1 Latest version!	Xenopus tropicalis phosphodiesterase 4B (pde4b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa29679	XM_031900281.1 Latest version!	Xenopus tropicalis phosphodiesterase 4B (pde4b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa29680	XM_031900282.1 Latest version!	Xenopus tropicalis phosphodiesterase 4B (pde4b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa29681	XM_012961422.2 Latest version!	Xenopus tropicalis phosphodiesterase 4B (pde4b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa29682	XM_012961423.3 Latest version!	Xenopus tropicalis phosphodiesterase 4B (pde4b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OXa29682	XM_012961423.2	Xenopus tropicalis phosphodiesterase 4B (pde4b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa29681	XM_012961422.1	Xenopus tropicalis phosphodiesterase 4B (pde4b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pde4b ( NM_001079395.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001072863.1 Xenopus tropicalis phosphodiesterase 4B (pde4b), mRNA.

Price & Availability↑

CloneID	OXa25098
Clone ID Related Accession (Same CDS sequence)	NM_001079395.1
Accession Version	NM_001079395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cAMP-specific 3',5'-cyclic phosphodiesterase 4B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC123100.1. ##Evidence-Data-START## Transcript exon combination :: BC123100.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN08563921 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGAAC ACTGTACAGC TTACAATACT GTGTGCCTCA GCTGTGGTGG GACTGAATCC 
ACCATGCAGA CCCTAGAGGA GCTGGAGCCT GATTACATGT CAATGTGCTT ATTTGCAGAG 
GATTCCTATC AAAAAATTGC AATGGACACT TTAGAAGAAC TTGACTGGTG CTTGGATCAA 
CTGGAGACTA TCCAGACCTA CAGATCGGTC AGTGAGATGG CATCTAACAA GTTCAAAAGG 
ATGTTGAACC GGGAGCTGAC CCACCTTTCA GAAATGAGCC GGTCAGGGAA CCAAGTGTCT 
GAGTACATCT CAAGCACATT TCTCGACAAA CAAAACGATG TGGAGATCCC ATCCCCAACA 
CAGAAAGACA GGGAAAAGAA GAAAAAACAG CAGCTTATGA CGCAGATTAG TGGAGTGAAA 
AAGTTAATGC ACAGTTCCAG CCTGAACAAT ACTAGCATGT CACGGTTCGG AGTAAAAACT 
GAATATGAAG ACATGCTCTC CAAGGAACTT GAAGACCTTA ATAAATGGGG ATTGAATATC 
TTCAAGGTTG CATCATACTC ATGCAATAGG CCCTTAACGT GCATCATGTA TGCCATATTT 
CAGGAAAGAG ACTTATTAAA GACATTTAAA ATACCAGCGG ATACTTTGAT AACCTACACA 
ATGACCTTGG AAGACCATTA TCATTCAGAT GTAGCATATC ACAACAGCCT GCATGCTGCT 
GATGTCACTC AGTCCACTCA TGTTCTTCTC TCCACTCCAG CACTGGATGC TGTCTTTACT 
GATTTGGAAA TCCTTGCTGC AATATTTGCA GCTGCTATTC ATGACGTGGA TCATCCGGGT 
GTCTCCAACC AGTTCCTTAT CAACACAAAT TCTGAGCTTG CTCTGATGTA CAATGATGAG 
TCCGTATTAG AAAATCATCA TCTTGCTGTG GGGTTTAAAT TATTACAAGA AGAGCATTGT 
GATATCTTTC AAAATCTTAC CAAGAAGCAA AGGCAGTCTC TTAGGAAAAT GGTGATTGAC 
ATGGTACTTG CTACTGATAT GTCTAAACAC ATGAGTCTGT TGGCTGACCT CAAGACTATG 
GTGGAAACCA AGAAAGTGAC AAGCTCCGGT GTTCTCCTTC TGGATAATTA CACTGACAGA 
ATACAGGTTC TCAGGAATAT GGTACACTGC GCAGATCTAA GCAATCCAAC TAAGTCACTG 
GAATTGTACA GACAATGGAC AGACCGAATT ATGGAGGAGT TCTTTCAGCA AGGAGATAAA 
GAGAGAGAAA GAGGGATGGA AATAAGCCCA ATGTGTGACA AACATACTGC TTCAGTGGAA 
AAGTCACAGG TTGGATTCAT TGACTACATT GTTCATCCTT TATGGGAAAC ATGGGCAGAT 
TTAGTTCAGC CAGATGCTCA AGATATTCTG GATACATTAG AGGATAATAG GAACTGGTAC 
CAAAGTATGA TACCTCAGAG TCCTTCTCCT CTTTTGGATG AACGTGATAA AGACTGCCAA 
GGGCTTTCAG AAAAGTTCCA TTTTGAGCTA ACCCTTGAGG AAGAAGATTC AGACGGGCAT 
GACAAAGATG GAGATTGCCT CAGTTTTTAT AGCAGTACAA AAACACTTCA TATAGTTGAT 
CAGGGGATGG AGGATTCACC AGATGCTGAC ACAGAATTCC TAACAGATGA TACATCACCT 
TTAGACACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072863.1
CDS	3..1694
Translation	MKEHCTAYNTVCLSCGGTESTMQTLEELEPDYMSMCLFAEDSYQKIAMDTLEELDWCLDQLETIQTYRSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISSTFLDKQNDVEIPSPTQKDREKKKKQQLMTQISGVKKLMHSSSLNNTSMSRFGVKTEYEDMLSKELEDLNKWGLNIFKVASYSCNRPLTCIMYAIFQERDLLKTFKIPADTLITYTMTLEDHYHSDVAYHNSLHAADVTQSTHVLLSTPALDAVFTDLEILAAIFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEEHCDIFQNLTKKQRQSLRKMVIDMVLATDMSKHMSLLADLKTMVETKKVTSSGVLLLDNYTDRIQVLRNMVHCADLSNPTKSLELYRQWTDRIMEEFFQQGDKERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVQPDAQDILDTLEDNRNWYQSMIPQSPSPLLDERDKDCQGLSEKFHFELTLEEEDSDGHDKDGDCLSFYSSTKTLHIVDQGMEDSPDADTEFLTDDTSPLDT

Target ORF information:

RefSeq Version	NM_001079395.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 4B (pde4b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079395.1

ORF Insert Sequence:

ATGAAGGAAC ACTGTACAGC TTACAATACT GTGTGCCTCA GCTGTGGTGG GACTGAATCC 
ACCATGCAGA CCCTAGAGGA GCTGGAGCCT GATTACATGT CAATGTGCTT ATTTGCAGAG 
GATTCCTATC AAAAAATTGC AATGGACACT TTAGAAGAAC TTGACTGGTG CTTGGATCAA 
CTGGAGACTA TCCAGACCTA CAGATCGGTC AGTGAGATGG CATCTAACAA GTTCAAAAGG 
ATGTTGAACC GGGAGCTGAC CCACCTTTCA GAAATGAGCC GGTCAGGGAA CCAAGTGTCT 
GAGTACATCT CAAGCACATT TCTCGACAAA CAAAACGATG TGGAGATCCC ATCCCCAACA 
CAGAAAGACA GGGAAAAGAA GAAAAAACAG CAGCTTATGA CGCAGATTAG TGGAGTGAAA 
AAGTTAATGC ACAGTTCCAG CCTGAACAAT ACTAGCATGT CACGGTTCGG AGTAAAAACT 
GAATATGAAG ACATGCTCTC CAAGGAACTT GAAGACCTTA ATAAATGGGG ATTGAATATC 
TTCAAGGTTG CATCATACTC ATGCAATAGG CCCTTAACGT GCATCATGTA TGCCATATTT 
CAGGAAAGAG ACTTATTAAA GACATTTAAA ATACCAGCGG ATACTTTGAT AACCTACACA 
ATGACCTTGG AAGACCATTA TCATTCAGAT GTAGCATATC ACAACAGCCT GCATGCTGCT 
GATGTCACTC AGTCCACTCA TGTTCTTCTC TCCACTCCAG CACTGGATGC TGTCTTTACT 
GATTTGGAAA TCCTTGCTGC AATATTTGCA GCTGCTATTC ATGACGTGGA TCATCCGGGT 
GTCTCCAACC AGTTCCTTAT CAACACAAAT TCTGAGCTTG CTCTGATGTA CAATGATGAG 
TCCGTATTAG AAAATCATCA TCTTGCTGTG GGGTTTAAAT TATTACAAGA AGAGCATTGT 
GATATCTTTC AAAATCTTAC CAAGAAGCAA AGGCAGTCTC TTAGGAAAAT GGTGATTGAC 
ATGGTACTTG CTACTGATAT GTCTAAACAC ATGAGTCTGT TGGCTGACCT CAAGACTATG 
GTGGAAACCA AGAAAGTGAC AAGCTCCGGT GTTCTCCTTC TGGATAATTA CACTGACAGA 
ATACAGGTTC TCAGGAATAT GGTACACTGC GCAGATCTAA GCAATCCAAC TAAGTCACTG 
GAATTGTACA GACAATGGAC AGACCGAATT ATGGAGGAGT TCTTTCAGCA AGGAGATAAA 
GAGAGAGAAA GAGGGATGGA AATAAGCCCA ATGTGTGACA AACATACTGC TTCAGTGGAA 
AAGTCACAGG TTGGATTCAT TGACTACATT GTTCATCCTT TATGGGAAAC ATGGGCAGAT 
TTAGTTCAGC CAGATGCTCA AGATATTCTG GATACATTAG AGGATAATAG GAACTGGTAC 
CAAAGTATGA TACCTCAGAG TCCTTCTCCT CTTTTGGATG AACGTGATAA AGACTGCCAA 
GGGCTTTCAG AAAAGTTCCA TTTTGAGCTA ACCCTTGAGG AAGAAGATTC AGACGGGCAT 
GACAAAGATG GAGATTGCCT CAGTTTTTAT AGCAGTACAA AAACACTTCA TATAGTTGAT 
CAGGGGATGG AGGATTCACC AGATGCTGAC ACAGAATTCC TAACAGATGA TACATCACCT 
TTAGACACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde4b ( XM_012961422.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816876.1 Xenopus tropicalis phosphodiesterase 4B (pde4b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa29681
Clone ID Related Accession (Same CDS sequence)	XM_012961422.1 , XM_012961422.2
Accession Version	XM_012961422.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cAMP-specific 3',5'-cyclic phosphodiesterase 4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCA TTAAAGAATT TCCAAGCCGA CGTCACTCTT GGATATGCTT TGATGTTGAA 
AATGGCCCCA CCCCTGGACG TAGCCCTCTG GACCACCAAG CCAGCCCTGG CTCTGGACTG 
GTGCTGCACA CCAACTTCAC AAGTCATAAC CAGCGCAGAG AGTCCTTTCT CTATCGATCG 
GATAGTGACT ATGATTTATC ACCAAAGACT ATGTCCAGGA ATTCCTCTGC TGCCAGTGAG 
CAGCATGGAG ATGACTTGAT AGTCACACCT TTTGCTCAGG TGCTGGCGAG TTTGCGCAGT 
GTAAGAAATA ATTTTACAGT GTTGACCAAT GTACATGGAG CACCAAATAA GAGGTCACCA 
ATGACCACCC AACCTCAGAT CTCCAGAGTT AATCTTCAAG AGGATTCCTA TCAAAAAATT 
GCAATGGACA CTTTAGAAGA ACTTGACTGG TGCTTGGATC AACTGGAGAC TATCCAGACC 
TACAGATCGG TCAGTGAGAT GGCATCTAAC AAGTTCAAAA GGATGTTGAA CCGGGAGCTG 
ACCCACCTTT CAGAAATGAG CCGGTCAGGG AACCAAGTGT CTGAGTACAT CTCAAGCACA 
TTTCTCGACA AACAAAACGA TGTGGAGATC CCATCCCCAA CACAGAAAGA CAGGGAAAAG 
AAGAAAAAAC AGCAGCTTAT GACGCAGATT AGTGGAGTGA AAAAGTTAAT GCACAGTTCC 
AGCCTGAACA ATACTAGCAT GTCACGGTTC GGAGTAAAAA CTGAATATGA AGACATGCTC 
TCCAAGGAAC TTGAAGACCT TAATAAATGG GGATTGAATA TCTTCAAGGT TGCATCATAC 
TCATGCAATA GGCCCTTAAC GTGCATCATG TATGCCATAT TTCAGGAAAG AGACTTATTA 
AAGACATTTA AAATACCAGC GGATACTTTG ATAACCTACA CAATGACCTT GGAAGACCAT 
TATCATTCAG ATGTAGCATA TCACAACAGC CTGCATGCTG CTGATGTCAC TCAGTCCACT 
CATGTTCTTC TCTCCACTCC AGCACTGGAT GCTGTCTTTA CTGATTTGGA AATCCTTGCT 
GCAATATTTG CAGCTGCTAT TCATGACGTG GATCATCCGG GTGTCTCCAA CCAGTTCCTT 
ATCAACACAA ATTCTGAGCT TGCTCTGATG TACAATGATG AGTCCGTATT AGAAAATCAT 
CATCTTGCTG TGGGGTTTAA ATTATTACAA GAAGAGCATT GTGATATCTT TCAAAATCTT 
ACCAAGAAGC AAAGGCAGTC TCTTAGGAAA ATGGTGATTG ACATGGTACT TGCTACTGAT 
ATGTCTAAAC ACATGAGTCT GTTGGCTGAC CTCAAGACTA TGGTGGAAAC CAAGAAAGTG 
ACAAGCTCCG GTGTTCTCCT TCTGGATAAT TACACTGACA GAATACAGGT TCTCAGGAAT 
ATGGTACACT GCGCAGATCT AAGCAATCCA ACTAAGTCAC TGGAGTTGTA CAGACAATGG 
ACAGACCGAA TTATGGAGGA GTTCTTTCAG CAAGGAGATA AAGAGAGAGA AAGAGGGATG 
GAAATAAGCC CAATGTGTGA CAAACATACT GCTTCAGTGG AAAAGTCACA GGTTGGATTC 
ATTGACTACA TTGTTCATCC TTTATGGGAA ACATGGGCAG ATTTAGTTCA GCCAGATGCT 
CAAGATATTC TGGATACATT AGAGGATAAT AGGAACTGGT ACCAAAGTAT GATACCTCAG 
AGTCCTTCTC CTCTTTTGGA TGAACATGAT AAAGACTGCC AAGGGCTTTC AGAAAAGTTC 
CATTTTGAGC TAACCCTTGA GGAAGAAGAT TCAGACGGGC ATGACAAAGA TGGAGATTGC 
CTCAGTTTTT ATAGCAGTAC AAAAACACTT CATATAGTTG ATCAGGGGAT GGAGGATTCA 
CCAGATGCTG ACACAGAATT CCTAACAGAT GATACATCAC CTTTAGACAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816876.1
CDS	454..2427
Translation	MLSIKEFPSRRHSWICFDVENGPTPGRSPLDHQASPGSGLVLHTNFTSHNQRRESFLYRSDSDYDLSPKTMSRNSSAASEQHGDDLIVTPFAQVLASLRSVRNNFTVLTNVHGAPNKRSPMTTQPQISRVNLQEDSYQKIAMDTLEELDWCLDQLETIQTYRSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISSTFLDKQNDVEIPSPTQKDREKKKKQQLMTQISGVKKLMHSSSLNNTSMSRFGVKTEYEDMLSKELEDLNKWGLNIFKVASYSCNRPLTCIMYAIFQERDLLKTFKIPADTLITYTMTLEDHYHSDVAYHNSLHAADVTQSTHVLLSTPALDAVFTDLEILAAIFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEEHCDIFQNLTKKQRQSLRKMVIDMVLATDMSKHMSLLADLKTMVETKKVTSSGVLLLDNYTDRIQVLRNMVHCADLSNPTKSLELYRQWTDRIMEEFFQQGDKERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVQPDAQDILDTLEDNRNWYQSMIPQSPSPLLDEHDKDCQGLSEKFHFELTLEEEDSDGHDKDGDCLSFYSSTKTLHIVDQGMEDSPDADTEFLTDDTSPLDT

Target ORF information:

RefSeq Version	XM_012961422.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 4B (pde4b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961422.1

ORF Insert Sequence:

ATGCTGAGCA TTAAAGAATT TCCAAGCCGA CGTCACTCTT GGATATGCTT TGATGTTGAA 
AATGGCCCCA CCCCTGGACG TAGCCCTCTG GACCACCAAG CCAGCCCTGG CTCTGGACTG 
GTGCTGCACA CCAACTTCAC AAGTCATAAC CAGCGCAGAG AGTCCTTTCT CTATCGATCG 
GATAGTGACT ATGATTTATC ACCAAAGACT ATGTCCAGGA ATTCCTCTGC TGCCAGTGAG 
CAGCATGGAG ATGACTTGAT AGTCACACCT TTTGCTCAGG TGCTGGCGAG TTTGCGCAGT 
GTAAGAAATA ATTTTACAGT GTTGACCAAT GTACATGGAG CACCAAATAA GAGGTCACCA 
ATGACCACCC AACCTCAGAT CTCCAGAGTT AATCTTCAAG AGGATTCCTA TCAAAAAATT 
GCAATGGACA CTTTAGAAGA ACTTGACTGG TGCTTGGATC AACTGGAGAC TATCCAGACC 
TACAGATCGG TCAGTGAGAT GGCATCTAAC AAGTTCAAAA GGATGTTGAA CCGGGAGCTG 
ACCCACCTTT CAGAAATGAG CCGGTCAGGG AACCAAGTGT CTGAGTACAT CTCAAGCACA 
TTTCTCGACA AACAAAACGA TGTGGAGATC CCATCCCCAA CACAGAAAGA CAGGGAAAAG 
AAGAAAAAAC AGCAGCTTAT GACGCAGATT AGTGGAGTGA AAAAGTTAAT GCACAGTTCC 
AGCCTGAACA ATACTAGCAT GTCACGGTTC GGAGTAAAAA CTGAATATGA AGACATGCTC 
TCCAAGGAAC TTGAAGACCT TAATAAATGG GGATTGAATA TCTTCAAGGT TGCATCATAC 
TCATGCAATA GGCCCTTAAC GTGCATCATG TATGCCATAT TTCAGGAAAG AGACTTATTA 
AAGACATTTA AAATACCAGC GGATACTTTG ATAACCTACA CAATGACCTT GGAAGACCAT 
TATCATTCAG ATGTAGCATA TCACAACAGC CTGCATGCTG CTGATGTCAC TCAGTCCACT 
CATGTTCTTC TCTCCACTCC AGCACTGGAT GCTGTCTTTA CTGATTTGGA AATCCTTGCT 
GCAATATTTG CAGCTGCTAT TCATGACGTG GATCATCCGG GTGTCTCCAA CCAGTTCCTT 
ATCAACACAA ATTCTGAGCT TGCTCTGATG TACAATGATG AGTCCGTATT AGAAAATCAT 
CATCTTGCTG TGGGGTTTAA ATTATTACAA GAAGAGCATT GTGATATCTT TCAAAATCTT 
ACCAAGAAGC AAAGGCAGTC TCTTAGGAAA ATGGTGATTG ACATGGTACT TGCTACTGAT 
ATGTCTAAAC ACATGAGTCT GTTGGCTGAC CTCAAGACTA TGGTGGAAAC CAAGAAAGTG 
ACAAGCTCCG GTGTTCTCCT TCTGGATAAT TACACTGACA GAATACAGGT TCTCAGGAAT 
ATGGTACACT GCGCAGATCT AAGCAATCCA ACTAAGTCAC TGGAGTTGTA CAGACAATGG 
ACAGACCGAA TTATGGAGGA GTTCTTTCAG CAAGGAGATA AAGAGAGAGA AAGAGGGATG 
GAAATAAGCC CAATGTGTGA CAAACATACT GCTTCAGTGG AAAAGTCACA GGTTGGATTC 
ATTGACTACA TTGTTCATCC TTTATGGGAA ACATGGGCAG ATTTAGTTCA GCCAGATGCT 
CAAGATATTC TGGATACATT AGAGGATAAT AGGAACTGGT ACCAAAGTAT GATACCTCAG 
AGTCCTTCTC CTCTTTTGGA TGAACATGAT AAAGACTGCC AAGGGCTTTC AGAAAAGTTC 
CATTTTGAGC TAACCCTTGA GGAAGAAGAT TCAGACGGGC ATGACAAAGA TGGAGATTGC 
CTCAGTTTTT ATAGCAGTAC AAAAACACTT CATATAGTTG ATCAGGGGAT GGAGGATTCA 
CCAGATGCTG ACACAGAATT CCTAACAGAT GATACATCAC CTTTAGACAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde4b ( XM_012961423.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816877.1 Xenopus tropicalis phosphodiesterase 4B (pde4b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa29682
Clone ID Related Accession (Same CDS sequence)	XM_012961423.3 , XM_012961423.2
Accession Version	XM_012961423.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cAMP-specific 3',5'-cyclic phosphodiesterase 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961423.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGAAG CAAATTATTT GCTTTCTGTG TCCTGGGGCT ACATCAAGTT CAAAAGGATG 
TTGAACCGGG AGCTGACCCA CCTTTCAGAA ATGAGCCGGT CAGGGAACCA AGTGTCTGAG 
TACATCTCAA GCACATTTCT CGACAAACAA AACGATGTGG AGATCCCATC CCCAACACAG 
AAAGACAGGG AAAAGAAGAA AAAACAGCAG CTTATGACGC AGATTAGTGG AGTGAAAAAG 
TTAATGCACA GTTCCAGCCT GAACAATACT AGCATGTCAC GGTTCGGAGT AAAAACTGAA 
TATGAAGACA TGCTCTCCAA GGAACTTGAA GACCTTAATA AATGGGGATT GAATATCTTC 
AAGGTTGCAT CATACTCATG CAATAGGCCC TTAACGTGCA TCATGTATGC CATATTTCAG 
GAAAGAGACT TATTAAAGAC ATTTAAAATA CCAGCGGATA CTTTGATAAC CTACACAATG 
ACCTTGGAAG ACCATTATCA TTCAGATGTA GCATATCACA ACAGCCTGCA TGCTGCTGAT 
GTCACTCAGT CCACTCATGT TCTTCTCTCC ACTCCAGCAC TGGATGCTGT CTTTACTGAT 
TTGGAAATCC TTGCTGCAAT ATTTGCAGCT GCTATTCATG ACGTGGATCA TCCGGGTGTC 
TCCAACCAGT TCCTTATCAA CACAAATTCT GAGCTTGCTC TGATGTACAA TGATGAGTCC 
GTATTAGAAA ATCATCATCT TGCTGTGGGG TTTAAATTAT TACAAGAAGA GCATTGTGAT 
ATCTTTCAAA ATCTTACCAA GAAGCAAAGG CAGTCTCTTA GGAAAATGGT GATTGACATG 
GTACTTGCTA CTGATATGTC TAAACACATG AGTCTGTTGG CTGACCTCAA GACTATGGTG 
GAAACCAAGA AAGTGACAAG CTCCGGTGTT CTCCTTCTGG ATAATTACAC TGACAGAATA 
CAGGTTCTCA GGAATATGGT ACACTGCGCA GATCTAAGCA ATCCAACTAA GTCACTGGAG 
TTGTACAGAC AATGGACAGA CCGAATTATG GAGGAGTTCT TTCAGCAAGG AGATAAAGAG 
AGAGAAAGAG GGATGGAAAT AAGCCCAATG TGTGACAAAC ATACTGCTTC AGTGGAAAAG 
TCACAGGTTG GATTCATTGA CTACATTGTT CATCCTTTAT GGGAAACATG GGCAGATTTA 
GTTCAGCCAG ATGCTCAAGA TATTCTGGAT ACATTAGAGG ATAATAGGAA CTGGTACCAA 
AGTATGATAC CTCAGAGTCC TTCTCCTCTT TTGGATGAAC ATGATAAAGA CTGCCAAGGG 
CTTTCAGAAA AGTTCCATTT TGAGCTAACC CTTGAGGAAG AAGATTCAGA CGGGCATGAC 
AAAGATGGAG ATTGCCTCAG TTTTTATAGC AGTACAAAAA CACTTCATAT AGTTGATCAG 
GGGATGGAGG ATTCACCAGA TGCTGACACA GAATTCCTAA CAGATGATAC ATCACCTTTA 
GACACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816877.1
CDS	519..2027
Translation	MPEANYLLSVSWGYIKFKRMLNRELTHLSEMSRSGNQVSEYISSTFLDKQNDVEIPSPTQKDREKKKKQQLMTQISGVKKLMHSSSLNNTSMSRFGVKTEYEDMLSKELEDLNKWGLNIFKVASYSCNRPLTCIMYAIFQERDLLKTFKIPADTLITYTMTLEDHYHSDVAYHNSLHAADVTQSTHVLLSTPALDAVFTDLEILAAIFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEEHCDIFQNLTKKQRQSLRKMVIDMVLATDMSKHMSLLADLKTMVETKKVTSSGVLLLDNYTDRIQVLRNMVHCADLSNPTKSLELYRQWTDRIMEEFFQQGDKERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVQPDAQDILDTLEDNRNWYQSMIPQSPSPLLDEHDKDCQGLSEKFHFELTLEEEDSDGHDKDGDCLSFYSSTKTLHIVDQGMEDSPDADTEFLTDDTSPLDT

Target ORF information:

RefSeq Version	XM_012961423.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 4B (pde4b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961423.2

ORF Insert Sequence:

ATGCCTGAAG CAAATTATTT GCTTTCTGTG TCCTGGGGCT ACATCAAGTT CAAAAGGATG 
TTGAACCGGG AGCTGACCCA CCTTTCAGAA ATGAGCCGGT CAGGGAACCA AGTGTCTGAG 
TACATCTCAA GCACATTTCT CGACAAACAA AACGATGTGG AGATCCCATC CCCAACACAG 
AAAGACAGGG AAAAGAAGAA AAAACAGCAG CTTATGACGC AGATTAGTGG AGTGAAAAAG 
TTAATGCACA GTTCCAGCCT GAACAATACT AGCATGTCAC GGTTCGGAGT AAAAACTGAA 
TATGAAGACA TGCTCTCCAA GGAACTTGAA GACCTTAATA AATGGGGATT GAATATCTTC 
AAGGTTGCAT CATACTCATG CAATAGGCCC TTAACGTGCA TCATGTATGC CATATTTCAG 
GAAAGAGACT TATTAAAGAC ATTTAAAATA CCAGCGGATA CTTTGATAAC CTACACAATG 
ACCTTGGAAG ACCATTATCA TTCAGATGTA GCATATCACA ACAGCCTGCA TGCTGCTGAT 
GTCACTCAGT CCACTCATGT TCTTCTCTCC ACTCCAGCAC TGGATGCTGT CTTTACTGAT 
TTGGAAATCC TTGCTGCAAT ATTTGCAGCT GCTATTCATG ACGTGGATCA TCCGGGTGTC 
TCCAACCAGT TCCTTATCAA CACAAATTCT GAGCTTGCTC TGATGTACAA TGATGAGTCC 
GTATTAGAAA ATCATCATCT TGCTGTGGGG TTTAAATTAT TACAAGAAGA GCATTGTGAT 
ATCTTTCAAA ATCTTACCAA GAAGCAAAGG CAGTCTCTTA GGAAAATGGT GATTGACATG 
GTACTTGCTA CTGATATGTC TAAACACATG AGTCTGTTGG CTGACCTCAA GACTATGGTG 
GAAACCAAGA AAGTGACAAG CTCCGGTGTT CTCCTTCTGG ATAATTACAC TGACAGAATA 
CAGGTTCTCA GGAATATGGT ACACTGCGCA GATCTAAGCA ATCCAACTAA GTCACTGGAG 
TTGTACAGAC AATGGACAGA CCGAATTATG GAGGAGTTCT TTCAGCAAGG AGATAAAGAG 
AGAGAAAGAG GGATGGAAAT AAGCCCAATG TGTGACAAAC ATACTGCTTC AGTGGAAAAG 
TCACAGGTTG GATTCATTGA CTACATTGTT CATCCTTTAT GGGAAACATG GGCAGATTTA 
GTTCAGCCAG ATGCTCAAGA TATTCTGGAT ACATTAGAGG ATAATAGGAA CTGGTACCAA 
AGTATGATAC CTCAGAGTCC TTCTCCTCTT TTGGATGAAC ATGATAAAGA CTGCCAAGGG 
CTTTCAGAAA AGTTCCATTT TGAGCTAACC CTTGAGGAAG AAGATTCAGA CGGGCATGAC 
AAAGATGGAG ATTGCCTCAG TTTTTATAGC AGTACAAAAA CACTTCATAT AGTTGATCAG 
GGGATGGAGG ATTCACCAGA TGCTGACACA GAATTCCTAA CAGATGATAC ATCACCTTTA 
GACACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde4b ( XM_031900281.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756141.1 Xenopus tropicalis phosphodiesterase 4B (pde4b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa29679
Clone ID Related Accession (Same CDS sequence)	XM_031900281.1
Accession Version	XM_031900281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cAMP-specific 3',5'-cyclic phosphodiesterase 4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGA GCAGAAGTGT AATGACAGTA ACGGCAGAAG ATAATGTCCA GAATTTCACT 
GAGGTTTGTC TGAGCAAGTC CTATAGCTCC TATGGGTACA ACATGGGGAT AGACCTTTGG 
AAAGGAAAAA GATTCTGCTC AGGGACATTG CAGTTACCCC CATTACATCA CAGGCAAATG 
AGAAGGGCAA AGACACCAGA CTACATCCAC AGGCCTACAA CTTTGCCACT GCTTCTTCCT 
CCGAAGATTG CTGTCACTCC TGTAGAGAAA GACAGCTTTG ATGTTGAAAA TGGCCCCACC 
CCTGGACGTA GCCCTCTGGA CCACCAAGCC AGCCCTGGCT CTGGACTGGT GCTGCACACC 
AACTTCACAA GTCATAACCA GCGCAGAGAG TCCTTTCTCT ATCGATCGGA TAGTGACTAT 
GATTTATCAC CAAAGACTAT GTCCAGGAAT TCCTCTGCTG CCAGTGAGCA GCATGGAGAT 
GACTTGATAG TCACACCTTT TGCTCAGGTG CTGGCGAGTT TGCGCAGTGT AAGAAATAAT 
TTTACAGTGT TGACCAATGT ACATGGAGCA CCAAATAAGA GGTCACCAAT GACCACCCAA 
CCTCAGATCT CCAGAGTTAA TCTTCAAGAG GATTCCTATC AAAAAATTGC AATGGACACT 
TTAGAAGAAC TTGACTGGTG CTTGGATCAA CTGGAGACTA TCCAGACCTA CAGATCGGTC 
AGTGAGATGG CATCTAACAA GTTCAAAAGG ATGTTGAACC GGGAGCTGAC CCACCTTTCA 
GAAATGAGCC GGTCAGGGAA CCAAGTGTCT GAGTACATCT CAAGCACATT TCTCGACAAA 
CAAAACGATG TGGAGATCCC ATCCCCAACA CAGAAAGACA GGGAAAAGAA GAAAAAACAG 
CAGCTTATGA CGCAGATTAG TGGAGTGAAA AAGTTAATGC ACAGTTCCAG CCTGAACAAT 
ACTAGCATGT CACGGTTCGG AGTAAAAACT GAATATGAAG ACATGCTCTC CAAGGAACTT 
GAAGACCTTA ATAAATGGGG ATTGAATATC TTCAAGGTTG CATCATACTC ATGCAATAGG 
CCCTTAACGT GCATCATGTA TGCCATATTT CAGGAAAGAG ACTTATTAAA GACATTTAAA 
ATACCAGCGG ATACTTTGAT AACCTACACA ATGACCTTGG AAGACCATTA TCATTCAGAT 
GTAGCATATC ACAACAGCCT GCATGCTGCT GATGTCACTC AGTCCACTCA TGTTCTTCTC 
TCCACTCCAG CACTGGATGC TGTCTTTACT GATTTGGAAA TCCTTGCTGC AATATTTGCA 
GCTGCTATTC ATGACGTGGA TCATCCGGGT GTCTCCAACC AGTTCCTTAT CAACACAAAT 
TCTGAGCTTG CTCTGATGTA CAATGATGAG TCCGTATTAG AAAATCATCA TCTTGCTGTG 
GGGTTTAAAT TATTACAAGA AGAGCATTGT GATATCTTTC AAAATCTTAC CAAGAAGCAA 
AGGCAGTCTC TTAGGAAAAT GGTGATTGAC ATGGTACTTG CTACTGATAT GTCTAAACAC 
ATGAGTCTGT TGGCTGACCT CAAGACTATG GTGGAAACCA AGAAAGTGAC AAGCTCCGGT 
GTTCTCCTTC TGGATAATTA CACTGACAGA ATACAGGTTC TCAGGAATAT GGTACACTGC 
GCAGATCTAA GCAATCCAAC TAAGTCACTG GAGTTGTACA GACAATGGAC AGACCGAATT 
ATGGAGGAGT TCTTTCAGCA AGGAGATAAA GAGAGAGAAA GAGGGATGGA AATAAGCCCA 
ATGTGTGACA AACATACTGC TTCAGTGGAA AAGTCACAGG TTGGATTCAT TGACTACATT 
GTTCATCCTT TATGGGAAAC ATGGGCAGAT TTAGTTCAGC CAGATGCTCA AGATATTCTG 
GATACATTAG AGGATAATAG GAACTGGTAC CAAAGTATGA TACCTCAGAG TCCTTCTCCT 
CTTTTGGATG AACATGATAA AGACTGCCAA GGGCTTTCAG AAAAGTTCCA TTTTGAGCTA 
ACCCTTGAGG AAGAAGATTC AGACGGGCAT GACAAAGATG GAGATTGCCT CAGTTTTTAT 
AGCAGTACAA AAACACTTCA TATAGTTGAT CAGGGGATGG AGGATTCACC AGATGCTGAC 
ACAGAATTCC TAACAGATGA TACATCACCT TTAGACACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756141.1
CDS	221..2422
Translation	MKKSRSVMTVTAEDNVQNFTEVCLSKSYSSYGYNMGIDLWKGKRFCSGTLQLPPLHHRQMRRAKTPDYIHRPTTLPLLLPPKIAVTPVEKDSFDVENGPTPGRSPLDHQASPGSGLVLHTNFTSHNQRRESFLYRSDSDYDLSPKTMSRNSSAASEQHGDDLIVTPFAQVLASLRSVRNNFTVLTNVHGAPNKRSPMTTQPQISRVNLQEDSYQKIAMDTLEELDWCLDQLETIQTYRSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISSTFLDKQNDVEIPSPTQKDREKKKKQQLMTQISGVKKLMHSSSLNNTSMSRFGVKTEYEDMLSKELEDLNKWGLNIFKVASYSCNRPLTCIMYAIFQERDLLKTFKIPADTLITYTMTLEDHYHSDVAYHNSLHAADVTQSTHVLLSTPALDAVFTDLEILAAIFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEEHCDIFQNLTKKQRQSLRKMVIDMVLATDMSKHMSLLADLKTMVETKKVTSSGVLLLDNYTDRIQVLRNMVHCADLSNPTKSLELYRQWTDRIMEEFFQQGDKERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVQPDAQDILDTLEDNRNWYQSMIPQSPSPLLDEHDKDCQGLSEKFHFELTLEEEDSDGHDKDGDCLSFYSSTKTLHIVDQGMEDSPDADTEFLTDDTSPLDT

Target ORF information:

RefSeq Version	XM_031900281.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 4B (pde4b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900281.1

ORF Insert Sequence:

ATGAAGAAGA GCAGAAGTGT AATGACAGTA ACGGCAGAAG ATAATGTCCA GAATTTCACT 
GAGGTTTGTC TGAGCAAGTC CTATAGCTCC TATGGGTACA ACATGGGGAT AGACCTTTGG 
AAAGGAAAAA GATTCTGCTC AGGGACATTG CAGTTACCCC CATTACATCA CAGGCAAATG 
AGAAGGGCAA AGACACCAGA CTACATCCAC AGGCCTACAA CTTTGCCACT GCTTCTTCCT 
CCGAAGATTG CTGTCACTCC TGTAGAGAAA GACAGCTTTG ATGTTGAAAA TGGCCCCACC 
CCTGGACGTA GCCCTCTGGA CCACCAAGCC AGCCCTGGCT CTGGACTGGT GCTGCACACC 
AACTTCACAA GTCATAACCA GCGCAGAGAG TCCTTTCTCT ATCGATCGGA TAGTGACTAT 
GATTTATCAC CAAAGACTAT GTCCAGGAAT TCCTCTGCTG CCAGTGAGCA GCATGGAGAT 
GACTTGATAG TCACACCTTT TGCTCAGGTG CTGGCGAGTT TGCGCAGTGT AAGAAATAAT 
TTTACAGTGT TGACCAATGT ACATGGAGCA CCAAATAAGA GGTCACCAAT GACCACCCAA 
CCTCAGATCT CCAGAGTTAA TCTTCAAGAG GATTCCTATC AAAAAATTGC AATGGACACT 
TTAGAAGAAC TTGACTGGTG CTTGGATCAA CTGGAGACTA TCCAGACCTA CAGATCGGTC 
AGTGAGATGG CATCTAACAA GTTCAAAAGG ATGTTGAACC GGGAGCTGAC CCACCTTTCA 
GAAATGAGCC GGTCAGGGAA CCAAGTGTCT GAGTACATCT CAAGCACATT TCTCGACAAA 
CAAAACGATG TGGAGATCCC ATCCCCAACA CAGAAAGACA GGGAAAAGAA GAAAAAACAG 
CAGCTTATGA CGCAGATTAG TGGAGTGAAA AAGTTAATGC ACAGTTCCAG CCTGAACAAT 
ACTAGCATGT CACGGTTCGG AGTAAAAACT GAATATGAAG ACATGCTCTC CAAGGAACTT 
GAAGACCTTA ATAAATGGGG ATTGAATATC TTCAAGGTTG CATCATACTC ATGCAATAGG 
CCCTTAACGT GCATCATGTA TGCCATATTT CAGGAAAGAG ACTTATTAAA GACATTTAAA 
ATACCAGCGG ATACTTTGAT AACCTACACA ATGACCTTGG AAGACCATTA TCATTCAGAT 
GTAGCATATC ACAACAGCCT GCATGCTGCT GATGTCACTC AGTCCACTCA TGTTCTTCTC 
TCCACTCCAG CACTGGATGC TGTCTTTACT GATTTGGAAA TCCTTGCTGC AATATTTGCA 
GCTGCTATTC ATGACGTGGA TCATCCGGGT GTCTCCAACC AGTTCCTTAT CAACACAAAT 
TCTGAGCTTG CTCTGATGTA CAATGATGAG TCCGTATTAG AAAATCATCA TCTTGCTGTG 
GGGTTTAAAT TATTACAAGA AGAGCATTGT GATATCTTTC AAAATCTTAC CAAGAAGCAA 
AGGCAGTCTC TTAGGAAAAT GGTGATTGAC ATGGTACTTG CTACTGATAT GTCTAAACAC 
ATGAGTCTGT TGGCTGACCT CAAGACTATG GTGGAAACCA AGAAAGTGAC AAGCTCCGGT 
GTTCTCCTTC TGGATAATTA CACTGACAGA ATACAGGTTC TCAGGAATAT GGTACACTGC 
GCAGATCTAA GCAATCCAAC TAAGTCACTG GAGTTGTACA GACAATGGAC AGACCGAATT 
ATGGAGGAGT TCTTTCAGCA AGGAGATAAA GAGAGAGAAA GAGGGATGGA AATAAGCCCA 
ATGTGTGACA AACATACTGC TTCAGTGGAA AAGTCACAGG TTGGATTCAT TGACTACATT 
GTTCATCCTT TATGGGAAAC ATGGGCAGAT TTAGTTCAGC CAGATGCTCA AGATATTCTG 
GATACATTAG AGGATAATAG GAACTGGTAC CAAAGTATGA TACCTCAGAG TCCTTCTCCT 
CTTTTGGATG AACATGATAA AGACTGCCAA GGGCTTTCAG AAAAGTTCCA TTTTGAGCTA 
ACCCTTGAGG AAGAAGATTC AGACGGGCAT GACAAAGATG GAGATTGCCT CAGTTTTTAT 
AGCAGTACAA AAACACTTCA TATAGTTGAT CAGGGGATGG AGGATTCACC AGATGCTGAC 
ACAGAATTCC TAACAGATGA TACATCACCT TTAGACACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde4b ( XM_031900282.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756142.1 Xenopus tropicalis phosphodiesterase 4B (pde4b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa29680
Clone ID Related Accession (Same CDS sequence)	XM_031900282.1
Accession Version	XM_031900282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cAMP-specific 3',5'-cyclic phosphodiesterase 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCAA AAGATTCTTC CAATGAACTT GCTACACCAG ATTCAGATAT ATACATAAGG 
ACTTTTAAAG AACATATGCA CTTAGAACTG GAACTTCCCA GACTTTCTGG GAAAAGGACC 
ACAACATCCC CAAAAATCTC TCCCAGAAGC TCTCCAAGGA ATTCACCGTG CTTTTTCAGA 
AAGTTGCTCG TTAATAAAAG CATTCGTCAG CGGCGGCGTT TTACAGTTGC ACATACATGC 
TTTGATGTTG AAAATGGCCC CACCCCTGGA CGTAGCCCTC TGGACCACCA AGCCAGCCCT 
GGCTCTGGAC TGGTGCTGCA CACCAACTTC ACAAGTCATA ACCAGCGCAG AGAGTCCTTT 
CTCTATCGAT CGGATAGTGA CTATGATTTA TCACCAAAGA CTATGTCCAG GAATTCCTCT 
GCTGCCAGTG AGCAGCATGG AGATGACTTG ATAGTCACAC CTTTTGCTCA GGTGCTGGCG 
AGTTTGCGCA GTGTAAGAAA TAATTTTACA GTGTTGACCA ATGTACATGG AGCACCAAAT 
AAGAGGTCAC CAATGACCAC CCAACCTCAG ATCTCCAGAG TTAATCTTCA AGAGGATTCC 
TATCAAAAAA TTGCAATGGA CACTTTAGAA GAACTTGACT GGTGCTTGGA TCAACTGGAG 
ACTATCCAGA CCTACAGATC GGTCAGTGAG ATGGCATCTA ACAAGTTCAA AAGGATGTTG 
AACCGGGAGC TGACCCACCT TTCAGAAATG AGCCGGTCAG GGAACCAAGT GTCTGAGTAC 
ATCTCAAGCA CATTTCTCGA CAAACAAAAC GATGTGGAGA TCCCATCCCC AACACAGAAA 
GACAGGGAAA AGAAGAAAAA ACAGCAGCTT ATGACGCAGA TTAGTGGAGT GAAAAAGTTA 
ATGCACAGTT CCAGCCTGAA CAATACTAGC ATGTCACGGT TCGGAGTAAA AACTGAATAT 
GAAGACATGC TCTCCAAGGA ACTTGAAGAC CTTAATAAAT GGGGATTGAA TATCTTCAAG 
GTTGCATCAT ACTCATGCAA TAGGCCCTTA ACGTGCATCA TGTATGCCAT ATTTCAGGAA 
AGAGACTTAT TAAAGACATT TAAAATACCA GCGGATACTT TGATAACCTA CACAATGACC 
TTGGAAGACC ATTATCATTC AGATGTAGCA TATCACAACA GCCTGCATGC TGCTGATGTC 
ACTCAGTCCA CTCATGTTCT TCTCTCCACT CCAGCACTGG ATGCTGTCTT TACTGATTTG 
GAAATCCTTG CTGCAATATT TGCAGCTGCT ATTCATGACG TGGATCATCC GGGTGTCTCC 
AACCAGTTCC TTATCAACAC AAATTCTGAG CTTGCTCTGA TGTACAATGA TGAGTCCGTA 
TTAGAAAATC ATCATCTTGC TGTGGGGTTT AAATTATTAC AAGAAGAGCA TTGTGATATC 
TTTCAAAATC TTACCAAGAA GCAAAGGCAG TCTCTTAGGA AAATGGTGAT TGACATGGTA 
CTTGCTACTG ATATGTCTAA ACACATGAGT CTGTTGGCTG ACCTCAAGAC TATGGTGGAA 
ACCAAGAAAG TGACAAGCTC CGGTGTTCTC CTTCTGGATA ATTACACTGA CAGAATACAG 
GTTCTCAGGA ATATGGTACA CTGCGCAGAT CTAAGCAATC CAACTAAGTC ACTGGAGTTG 
TACAGACAAT GGACAGACCG AATTATGGAG GAGTTCTTTC AGCAAGGAGA TAAAGAGAGA 
GAAAGAGGGA TGGAAATAAG CCCAATGTGT GACAAACATA CTGCTTCAGT GGAAAAGTCA 
CAGGTTGGAT TCATTGACTA CATTGTTCAT CCTTTATGGG AAACATGGGC AGATTTAGTT 
CAGCCAGATG CTCAAGATAT TCTGGATACA TTAGAGGATA ATAGGAACTG GTACCAAAGT 
ATGATACCTC AGAGTCCTTC TCCTCTTTTG GATGAACATG ATAAAGACTG CCAAGGGCTT 
TCAGAAAAGT TCCATTTTGA GCTAACCCTT GAGGAAGAAG ATTCAGACGG GCATGACAAA 
GATGGAGATT GCCTCAGTTT TTATAGCAGT ACAAAAACAC TTCATATAGT TGATCAGGGG 
ATGGAGGATT CACCAGATGC TGACACAGAA TTCCTAACAG ATGATACATC ACCTTTAGAC 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756142.1
CDS	444..2609
Translation	MTAKDSSNELATPDSDIYIRTFKEHMHLELELPRLSGKRTTTSPKISPRSSPRNSPCFFRKLLVNKSIRQRRRFTVAHTCFDVENGPTPGRSPLDHQASPGSGLVLHTNFTSHNQRRESFLYRSDSDYDLSPKTMSRNSSAASEQHGDDLIVTPFAQVLASLRSVRNNFTVLTNVHGAPNKRSPMTTQPQISRVNLQEDSYQKIAMDTLEELDWCLDQLETIQTYRSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISSTFLDKQNDVEIPSPTQKDREKKKKQQLMTQISGVKKLMHSSSLNNTSMSRFGVKTEYEDMLSKELEDLNKWGLNIFKVASYSCNRPLTCIMYAIFQERDLLKTFKIPADTLITYTMTLEDHYHSDVAYHNSLHAADVTQSTHVLLSTPALDAVFTDLEILAAIFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEEHCDIFQNLTKKQRQSLRKMVIDMVLATDMSKHMSLLADLKTMVETKKVTSSGVLLLDNYTDRIQVLRNMVHCADLSNPTKSLELYRQWTDRIMEEFFQQGDKERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVQPDAQDILDTLEDNRNWYQSMIPQSPSPLLDEHDKDCQGLSEKFHFELTLEEEDSDGHDKDGDCLSFYSSTKTLHIVDQGMEDSPDADTEFLTDDTSPLDT

Target ORF information:

RefSeq Version	XM_031900282.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 4B (pde4b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900282.1

ORF Insert Sequence:

ATGACAGCAA AAGATTCTTC CAATGAACTT GCTACACCAG ATTCAGATAT ATACATAAGG 
ACTTTTAAAG AACATATGCA CTTAGAACTG GAACTTCCCA GACTTTCTGG GAAAAGGACC 
ACAACATCCC CAAAAATCTC TCCCAGAAGC TCTCCAAGGA ATTCACCGTG CTTTTTCAGA 
AAGTTGCTCG TTAATAAAAG CATTCGTCAG CGGCGGCGTT TTACAGTTGC ACATACATGC 
TTTGATGTTG AAAATGGCCC CACCCCTGGA CGTAGCCCTC TGGACCACCA AGCCAGCCCT 
GGCTCTGGAC TGGTGCTGCA CACCAACTTC ACAAGTCATA ACCAGCGCAG AGAGTCCTTT 
CTCTATCGAT CGGATAGTGA CTATGATTTA TCACCAAAGA CTATGTCCAG GAATTCCTCT 
GCTGCCAGTG AGCAGCATGG AGATGACTTG ATAGTCACAC CTTTTGCTCA GGTGCTGGCG 
AGTTTGCGCA GTGTAAGAAA TAATTTTACA GTGTTGACCA ATGTACATGG AGCACCAAAT 
AAGAGGTCAC CAATGACCAC CCAACCTCAG ATCTCCAGAG TTAATCTTCA AGAGGATTCC 
TATCAAAAAA TTGCAATGGA CACTTTAGAA GAACTTGACT GGTGCTTGGA TCAACTGGAG 
ACTATCCAGA CCTACAGATC GGTCAGTGAG ATGGCATCTA ACAAGTTCAA AAGGATGTTG 
AACCGGGAGC TGACCCACCT TTCAGAAATG AGCCGGTCAG GGAACCAAGT GTCTGAGTAC 
ATCTCAAGCA CATTTCTCGA CAAACAAAAC GATGTGGAGA TCCCATCCCC AACACAGAAA 
GACAGGGAAA AGAAGAAAAA ACAGCAGCTT ATGACGCAGA TTAGTGGAGT GAAAAAGTTA 
ATGCACAGTT CCAGCCTGAA CAATACTAGC ATGTCACGGT TCGGAGTAAA AACTGAATAT 
GAAGACATGC TCTCCAAGGA ACTTGAAGAC CTTAATAAAT GGGGATTGAA TATCTTCAAG 
GTTGCATCAT ACTCATGCAA TAGGCCCTTA ACGTGCATCA TGTATGCCAT ATTTCAGGAA 
AGAGACTTAT TAAAGACATT TAAAATACCA GCGGATACTT TGATAACCTA CACAATGACC 
TTGGAAGACC ATTATCATTC AGATGTAGCA TATCACAACA GCCTGCATGC TGCTGATGTC 
ACTCAGTCCA CTCATGTTCT TCTCTCCACT CCAGCACTGG ATGCTGTCTT TACTGATTTG 
GAAATCCTTG CTGCAATATT TGCAGCTGCT ATTCATGACG TGGATCATCC GGGTGTCTCC 
AACCAGTTCC TTATCAACAC AAATTCTGAG CTTGCTCTGA TGTACAATGA TGAGTCCGTA 
TTAGAAAATC ATCATCTTGC TGTGGGGTTT AAATTATTAC AAGAAGAGCA TTGTGATATC 
TTTCAAAATC TTACCAAGAA GCAAAGGCAG TCTCTTAGGA AAATGGTGAT TGACATGGTA 
CTTGCTACTG ATATGTCTAA ACACATGAGT CTGTTGGCTG ACCTCAAGAC TATGGTGGAA 
ACCAAGAAAG TGACAAGCTC CGGTGTTCTC CTTCTGGATA ATTACACTGA CAGAATACAG 
GTTCTCAGGA ATATGGTACA CTGCGCAGAT CTAAGCAATC CAACTAAGTC ACTGGAGTTG 
TACAGACAAT GGACAGACCG AATTATGGAG GAGTTCTTTC AGCAAGGAGA TAAAGAGAGA 
GAAAGAGGGA TGGAAATAAG CCCAATGTGT GACAAACATA CTGCTTCAGT GGAAAAGTCA 
CAGGTTGGAT TCATTGACTA CATTGTTCAT CCTTTATGGG AAACATGGGC AGATTTAGTT 
CAGCCAGATG CTCAAGATAT TCTGGATACA TTAGAGGATA ATAGGAACTG GTACCAAAGT 
ATGATACCTC AGAGTCCTTC TCCTCTTTTG GATGAACATG ATAAAGACTG CCAAGGGCTT 
TCAGAAAAGT TCCATTTTGA GCTAACCCTT GAGGAAGAAG ATTCAGACGG GCATGACAAA 
GATGGAGATT GCCTCAGTTT TTATAGCAGT ACAAAAACAC TTCATATAGT TGATCAGGGG 
ATGGAGGATT CACCAGATGC TGACACAGAA TTCCTAACAG ATGATACATC ACCTTTAGAC 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde4b ( XM_012961422.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816876.1 Xenopus tropicalis phosphodiesterase 4B (pde4b), transcript variant X3, mRNA.

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CloneID	OXa29681
Clone ID Related Accession (Same CDS sequence)	XM_012961422.1 , XM_012961422.2
Accession Version	XM_012961422.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cAMP-specific 3',5'-cyclic phosphodiesterase 4B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCA TTAAAGAATT TCCAAGCCGA CGTCACTCTT GGATATGCTT TGATGTTGAA 
AATGGCCCCA CCCCTGGACG TAGCCCTCTG GACCACCAAG CCAGCCCTGG CTCTGGACTG 
GTGCTGCACA CCAACTTCAC AAGTCATAAC CAGCGCAGAG AGTCCTTTCT CTATCGATCG 
GATAGTGACT ATGATTTATC ACCAAAGACT ATGTCCAGGA ATTCCTCTGC TGCCAGTGAG 
CAGCATGGAG ATGACTTGAT AGTCACACCT TTTGCTCAGG TGCTGGCGAG TTTGCGCAGT 
GTAAGAAATA ATTTTACAGT GTTGACCAAT GTACATGGAG CACCAAATAA GAGGTCACCA 
ATGACCACCC AACCTCAGAT CTCCAGAGTT AATCTTCAAG AGGATTCCTA TCAAAAAATT 
GCAATGGACA CTTTAGAAGA ACTTGACTGG TGCTTGGATC AACTGGAGAC TATCCAGACC 
TACAGATCGG TCAGTGAGAT GGCATCTAAC AAGTTCAAAA GGATGTTGAA CCGGGAGCTG 
ACCCACCTTT CAGAAATGAG CCGGTCAGGG AACCAAGTGT CTGAGTACAT CTCAAGCACA 
TTTCTCGACA AACAAAACGA TGTGGAGATC CCATCCCCAA CACAGAAAGA CAGGGAAAAG 
AAGAAAAAAC AGCAGCTTAT GACGCAGATT AGTGGAGTGA AAAAGTTAAT GCACAGTTCC 
AGCCTGAACA ATACTAGCAT GTCACGGTTC GGAGTAAAAA CTGAATATGA AGACATGCTC 
TCCAAGGAAC TTGAAGACCT TAATAAATGG GGATTGAATA TCTTCAAGGT TGCATCATAC 
TCATGCAATA GGCCCTTAAC GTGCATCATG TATGCCATAT TTCAGGAAAG AGACTTATTA 
AAGACATTTA AAATACCAGC GGATACTTTG ATAACCTACA CAATGACCTT GGAAGACCAT 
TATCATTCAG ATGTAGCATA TCACAACAGC CTGCATGCTG CTGATGTCAC TCAGTCCACT 
CATGTTCTTC TCTCCACTCC AGCACTGGAT GCTGTCTTTA CTGATTTGGA AATCCTTGCT 
GCAATATTTG CAGCTGCTAT TCATGACGTG GATCATCCGG GTGTCTCCAA CCAGTTCCTT 
ATCAACACAA ATTCTGAGCT TGCTCTGATG TACAATGATG AGTCCGTATT AGAAAATCAT 
CATCTTGCTG TGGGGTTTAA ATTATTACAA GAAGAGCATT GTGATATCTT TCAAAATCTT 
ACCAAGAAGC AAAGGCAGTC TCTTAGGAAA ATGGTGATTG ACATGGTACT TGCTACTGAT 
ATGTCTAAAC ACATGAGTCT GTTGGCTGAC CTCAAGACTA TGGTGGAAAC CAAGAAAGTG 
ACAAGCTCCG GTGTTCTCCT TCTGGATAAT TACACTGACA GAATACAGGT TCTCAGGAAT 
ATGGTACACT GCGCAGATCT AAGCAATCCA ACTAAGTCAC TGGAGTTGTA CAGACAATGG 
ACAGACCGAA TTATGGAGGA GTTCTTTCAG CAAGGAGATA AAGAGAGAGA AAGAGGGATG 
GAAATAAGCC CAATGTGTGA CAAACATACT GCTTCAGTGG AAAAGTCACA GGTTGGATTC 
ATTGACTACA TTGTTCATCC TTTATGGGAA ACATGGGCAG ATTTAGTTCA GCCAGATGCT 
CAAGATATTC TGGATACATT AGAGGATAAT AGGAACTGGT ACCAAAGTAT GATACCTCAG 
AGTCCTTCTC CTCTTTTGGA TGAACATGAT AAAGACTGCC AAGGGCTTTC AGAAAAGTTC 
CATTTTGAGC TAACCCTTGA GGAAGAAGAT TCAGACGGGC ATGACAAAGA TGGAGATTGC 
CTCAGTTTTT ATAGCAGTAC AAAAACACTT CATATAGTTG ATCAGGGGAT GGAGGATTCA 
CCAGATGCTG ACACAGAATT CCTAACAGAT GATACATCAC CTTTAGACAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816876.1
CDS	446..2419
Translation	MLSIKEFPSRRHSWICFDVENGPTPGRSPLDHQASPGSGLVLHTNFTSHNQRRESFLYRSDSDYDLSPKTMSRNSSAASEQHGDDLIVTPFAQVLASLRSVRNNFTVLTNVHGAPNKRSPMTTQPQISRVNLQEDSYQKIAMDTLEELDWCLDQLETIQTYRSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISSTFLDKQNDVEIPSPTQKDREKKKKQQLMTQISGVKKLMHSSSLNNTSMSRFGVKTEYEDMLSKELEDLNKWGLNIFKVASYSCNRPLTCIMYAIFQERDLLKTFKIPADTLITYTMTLEDHYHSDVAYHNSLHAADVTQSTHVLLSTPALDAVFTDLEILAAIFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEEHCDIFQNLTKKQRQSLRKMVIDMVLATDMSKHMSLLADLKTMVETKKVTSSGVLLLDNYTDRIQVLRNMVHCADLSNPTKSLELYRQWTDRIMEEFFQQGDKERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVQPDAQDILDTLEDNRNWYQSMIPQSPSPLLDEHDKDCQGLSEKFHFELTLEEEDSDGHDKDGDCLSFYSSTKTLHIVDQGMEDSPDADTEFLTDDTSPLDT

Target ORF information:

RefSeq Version	XM_012961422.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 4B (pde4b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961422.2

ORF Insert Sequence:

ATGCTGAGCA TTAAAGAATT TCCAAGCCGA CGTCACTCTT GGATATGCTT TGATGTTGAA 
AATGGCCCCA CCCCTGGACG TAGCCCTCTG GACCACCAAG CCAGCCCTGG CTCTGGACTG 
GTGCTGCACA CCAACTTCAC AAGTCATAAC CAGCGCAGAG AGTCCTTTCT CTATCGATCG 
GATAGTGACT ATGATTTATC ACCAAAGACT ATGTCCAGGA ATTCCTCTGC TGCCAGTGAG 
CAGCATGGAG ATGACTTGAT AGTCACACCT TTTGCTCAGG TGCTGGCGAG TTTGCGCAGT 
GTAAGAAATA ATTTTACAGT GTTGACCAAT GTACATGGAG CACCAAATAA GAGGTCACCA 
ATGACCACCC AACCTCAGAT CTCCAGAGTT AATCTTCAAG AGGATTCCTA TCAAAAAATT 
GCAATGGACA CTTTAGAAGA ACTTGACTGG TGCTTGGATC AACTGGAGAC TATCCAGACC 
TACAGATCGG TCAGTGAGAT GGCATCTAAC AAGTTCAAAA GGATGTTGAA CCGGGAGCTG 
ACCCACCTTT CAGAAATGAG CCGGTCAGGG AACCAAGTGT CTGAGTACAT CTCAAGCACA 
TTTCTCGACA AACAAAACGA TGTGGAGATC CCATCCCCAA CACAGAAAGA CAGGGAAAAG 
AAGAAAAAAC AGCAGCTTAT GACGCAGATT AGTGGAGTGA AAAAGTTAAT GCACAGTTCC 
AGCCTGAACA ATACTAGCAT GTCACGGTTC GGAGTAAAAA CTGAATATGA AGACATGCTC 
TCCAAGGAAC TTGAAGACCT TAATAAATGG GGATTGAATA TCTTCAAGGT TGCATCATAC 
TCATGCAATA GGCCCTTAAC GTGCATCATG TATGCCATAT TTCAGGAAAG AGACTTATTA 
AAGACATTTA AAATACCAGC GGATACTTTG ATAACCTACA CAATGACCTT GGAAGACCAT 
TATCATTCAG ATGTAGCATA TCACAACAGC CTGCATGCTG CTGATGTCAC TCAGTCCACT 
CATGTTCTTC TCTCCACTCC AGCACTGGAT GCTGTCTTTA CTGATTTGGA AATCCTTGCT 
GCAATATTTG CAGCTGCTAT TCATGACGTG GATCATCCGG GTGTCTCCAA CCAGTTCCTT 
ATCAACACAA ATTCTGAGCT TGCTCTGATG TACAATGATG AGTCCGTATT AGAAAATCAT 
CATCTTGCTG TGGGGTTTAA ATTATTACAA GAAGAGCATT GTGATATCTT TCAAAATCTT 
ACCAAGAAGC AAAGGCAGTC TCTTAGGAAA ATGGTGATTG ACATGGTACT TGCTACTGAT 
ATGTCTAAAC ACATGAGTCT GTTGGCTGAC CTCAAGACTA TGGTGGAAAC CAAGAAAGTG 
ACAAGCTCCG GTGTTCTCCT TCTGGATAAT TACACTGACA GAATACAGGT TCTCAGGAAT 
ATGGTACACT GCGCAGATCT AAGCAATCCA ACTAAGTCAC TGGAGTTGTA CAGACAATGG 
ACAGACCGAA TTATGGAGGA GTTCTTTCAG CAAGGAGATA AAGAGAGAGA AAGAGGGATG 
GAAATAAGCC CAATGTGTGA CAAACATACT GCTTCAGTGG AAAAGTCACA GGTTGGATTC 
ATTGACTACA TTGTTCATCC TTTATGGGAA ACATGGGCAG ATTTAGTTCA GCCAGATGCT 
CAAGATATTC TGGATACATT AGAGGATAAT AGGAACTGGT ACCAAAGTAT GATACCTCAG 
AGTCCTTCTC CTCTTTTGGA TGAACATGAT AAAGACTGCC AAGGGCTTTC AGAAAAGTTC 
CATTTTGAGC TAACCCTTGA GGAAGAAGAT TCAGACGGGC ATGACAAAGA TGGAGATTGC 
CTCAGTTTTT ATAGCAGTAC AAAAACACTT CATATAGTTG ATCAGGGGAT GGAGGATTCA 
CCAGATGCTG ACACAGAATT CCTAACAGAT GATACATCAC CTTTAGACAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde4b ( XM_012961423.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816877.1 Xenopus tropicalis phosphodiesterase 4B (pde4b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa29682
Clone ID Related Accession (Same CDS sequence)	XM_012961423.3 , XM_012961423.2
Accession Version	XM_012961423.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cAMP-specific 3',5'-cyclic phosphodiesterase 4B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961423.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGAAG CAAATTATTT GCTTTCTGTG TCCTGGGGCT ACATCAAGTT CAAAAGGATG 
TTGAACCGGG AGCTGACCCA CCTTTCAGAA ATGAGCCGGT CAGGGAACCA AGTGTCTGAG 
TACATCTCAA GCACATTTCT CGACAAACAA AACGATGTGG AGATCCCATC CCCAACACAG 
AAAGACAGGG AAAAGAAGAA AAAACAGCAG CTTATGACGC AGATTAGTGG AGTGAAAAAG 
TTAATGCACA GTTCCAGCCT GAACAATACT AGCATGTCAC GGTTCGGAGT AAAAACTGAA 
TATGAAGACA TGCTCTCCAA GGAACTTGAA GACCTTAATA AATGGGGATT GAATATCTTC 
AAGGTTGCAT CATACTCATG CAATAGGCCC TTAACGTGCA TCATGTATGC CATATTTCAG 
GAAAGAGACT TATTAAAGAC ATTTAAAATA CCAGCGGATA CTTTGATAAC CTACACAATG 
ACCTTGGAAG ACCATTATCA TTCAGATGTA GCATATCACA ACAGCCTGCA TGCTGCTGAT 
GTCACTCAGT CCACTCATGT TCTTCTCTCC ACTCCAGCAC TGGATGCTGT CTTTACTGAT 
TTGGAAATCC TTGCTGCAAT ATTTGCAGCT GCTATTCATG ACGTGGATCA TCCGGGTGTC 
TCCAACCAGT TCCTTATCAA CACAAATTCT GAGCTTGCTC TGATGTACAA TGATGAGTCC 
GTATTAGAAA ATCATCATCT TGCTGTGGGG TTTAAATTAT TACAAGAAGA GCATTGTGAT 
ATCTTTCAAA ATCTTACCAA GAAGCAAAGG CAGTCTCTTA GGAAAATGGT GATTGACATG 
GTACTTGCTA CTGATATGTC TAAACACATG AGTCTGTTGG CTGACCTCAA GACTATGGTG 
GAAACCAAGA AAGTGACAAG CTCCGGTGTT CTCCTTCTGG ATAATTACAC TGACAGAATA 
CAGGTTCTCA GGAATATGGT ACACTGCGCA GATCTAAGCA ATCCAACTAA GTCACTGGAG 
TTGTACAGAC AATGGACAGA CCGAATTATG GAGGAGTTCT TTCAGCAAGG AGATAAAGAG 
AGAGAAAGAG GGATGGAAAT AAGCCCAATG TGTGACAAAC ATACTGCTTC AGTGGAAAAG 
TCACAGGTTG GATTCATTGA CTACATTGTT CATCCTTTAT GGGAAACATG GGCAGATTTA 
GTTCAGCCAG ATGCTCAAGA TATTCTGGAT ACATTAGAGG ATAATAGGAA CTGGTACCAA 
AGTATGATAC CTCAGAGTCC TTCTCCTCTT TTGGATGAAC ATGATAAAGA CTGCCAAGGG 
CTTTCAGAAA AGTTCCATTT TGAGCTAACC CTTGAGGAAG AAGATTCAGA CGGGCATGAC 
AAAGATGGAG ATTGCCTCAG TTTTTATAGC AGTACAAAAA CACTTCATAT AGTTGATCAG 
GGGATGGAGG ATTCACCAGA TGCTGACACA GAATTCCTAA CAGATGATAC ATCACCTTTA 
GACACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816877.1
CDS	621..2129
Translation	MPEANYLLSVSWGYIKFKRMLNRELTHLSEMSRSGNQVSEYISSTFLDKQNDVEIPSPTQKDREKKKKQQLMTQISGVKKLMHSSSLNNTSMSRFGVKTEYEDMLSKELEDLNKWGLNIFKVASYSCNRPLTCIMYAIFQERDLLKTFKIPADTLITYTMTLEDHYHSDVAYHNSLHAADVTQSTHVLLSTPALDAVFTDLEILAAIFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEEHCDIFQNLTKKQRQSLRKMVIDMVLATDMSKHMSLLADLKTMVETKKVTSSGVLLLDNYTDRIQVLRNMVHCADLSNPTKSLELYRQWTDRIMEEFFQQGDKERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVQPDAQDILDTLEDNRNWYQSMIPQSPSPLLDEHDKDCQGLSEKFHFELTLEEEDSDGHDKDGDCLSFYSSTKTLHIVDQGMEDSPDADTEFLTDDTSPLDT

Target ORF information:

RefSeq Version	XM_012961423.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 4B (pde4b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961423.3

ORF Insert Sequence:

ATGCCTGAAG CAAATTATTT GCTTTCTGTG TCCTGGGGCT ACATCAAGTT CAAAAGGATG 
TTGAACCGGG AGCTGACCCA CCTTTCAGAA ATGAGCCGGT CAGGGAACCA AGTGTCTGAG 
TACATCTCAA GCACATTTCT CGACAAACAA AACGATGTGG AGATCCCATC CCCAACACAG 
AAAGACAGGG AAAAGAAGAA AAAACAGCAG CTTATGACGC AGATTAGTGG AGTGAAAAAG 
TTAATGCACA GTTCCAGCCT GAACAATACT AGCATGTCAC GGTTCGGAGT AAAAACTGAA 
TATGAAGACA TGCTCTCCAA GGAACTTGAA GACCTTAATA AATGGGGATT GAATATCTTC 
AAGGTTGCAT CATACTCATG CAATAGGCCC TTAACGTGCA TCATGTATGC CATATTTCAG 
GAAAGAGACT TATTAAAGAC ATTTAAAATA CCAGCGGATA CTTTGATAAC CTACACAATG 
ACCTTGGAAG ACCATTATCA TTCAGATGTA GCATATCACA ACAGCCTGCA TGCTGCTGAT 
GTCACTCAGT CCACTCATGT TCTTCTCTCC ACTCCAGCAC TGGATGCTGT CTTTACTGAT 
TTGGAAATCC TTGCTGCAAT ATTTGCAGCT GCTATTCATG ACGTGGATCA TCCGGGTGTC 
TCCAACCAGT TCCTTATCAA CACAAATTCT GAGCTTGCTC TGATGTACAA TGATGAGTCC 
GTATTAGAAA ATCATCATCT TGCTGTGGGG TTTAAATTAT TACAAGAAGA GCATTGTGAT 
ATCTTTCAAA ATCTTACCAA GAAGCAAAGG CAGTCTCTTA GGAAAATGGT GATTGACATG 
GTACTTGCTA CTGATATGTC TAAACACATG AGTCTGTTGG CTGACCTCAA GACTATGGTG 
GAAACCAAGA AAGTGACAAG CTCCGGTGTT CTCCTTCTGG ATAATTACAC TGACAGAATA 
CAGGTTCTCA GGAATATGGT ACACTGCGCA GATCTAAGCA ATCCAACTAA GTCACTGGAG 
TTGTACAGAC AATGGACAGA CCGAATTATG GAGGAGTTCT TTCAGCAAGG AGATAAAGAG 
AGAGAAAGAG GGATGGAAAT AAGCCCAATG TGTGACAAAC ATACTGCTTC AGTGGAAAAG 
TCACAGGTTG GATTCATTGA CTACATTGTT CATCCTTTAT GGGAAACATG GGCAGATTTA 
GTTCAGCCAG ATGCTCAAGA TATTCTGGAT ACATTAGAGG ATAATAGGAA CTGGTACCAA 
AGTATGATAC CTCAGAGTCC TTCTCCTCTT TTGGATGAAC ATGATAAAGA CTGCCAAGGG 
CTTTCAGAAA AGTTCCATTT TGAGCTAACC CTTGAGGAAG AAGATTCAGA CGGGCATGAC 
AAAGATGGAG ATTGCCTCAG TTTTTATAGC AGTACAAAAA CACTTCATAT AGTTGATCAG 
GGGATGGAGG ATTCACCAGA TGCTGACACA GAATTCCTAA CAGATGATAC ATCACCTTTA 
GACACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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pde4b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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