cep170b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	cep170b
Entrez Gene ID	780180
Full Name	centrosomal protein 170B
Synonyms	kiaa0284
General protein information	Preferred Names centrosomal protein 170B Names centrosomal protein of 170 kDa protein B
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031890594.1	XP_031746454.1	centrosomal protein of 170 kDa protein B isoform X3
NM_001079255.1	NP_001072723.1	centrosomal protein of 170 kDa protein B
XM_031890593.1	XP_031746453.1	centrosomal protein of 170 kDa protein B isoform X2
XM_031890592.1	XP_031746452.1	centrosomal protein of 170 kDa protein B isoform X1
XM_031890595.1	XP_031746455.1	centrosomal protein of 170 kDa protein B isoform X4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	KIAA0284	XP_003952654.1
Canis lupus familiaris (dog)	KIAA0284	XP_005624002.1
Bos taurus (cattle)	KIAA0284	XP_002696837.2
Rattus norvegicus (Norway rat)	Cep170b	XP_576105.4
	LOC697998	XP_001085960.2
Gallus gallus (chicken)	KIAA0284	XP_004936461.1
Danio rerio (zebrafish)	si:ch73-212j7.1	XP_005160385.1
Homo sapiens (human)	CEP170B	NP_001106197.1
Mus musculus (house mouse)	Cep170b	NP_001019773.2
Xenopus tropicalis (tropical clawed frog)	cep170b	NP_001072723.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cep170b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cep170b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa63816	XM_031890592.1 Latest version!	Xenopus tropicalis centrosomal protein 170B (cep170b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa63817	XM_031890593.1 Latest version!	Xenopus tropicalis centrosomal protein 170B (cep170b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa63818	XM_031890594.1 Latest version!	Xenopus tropicalis centrosomal protein 170B (cep170b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa63819	XM_031890595.1 Latest version!	Xenopus tropicalis centrosomal protein 170B (cep170b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa22254	NM_001079255.1 Latest version!	Xenopus tropicalis centrosomal protein 170B (cep170b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cep170b ( XM_031890592.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746452.1 Xenopus tropicalis centrosomal protein 170B (cep170b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa63816
Clone ID Related Accession (Same CDS sequence)	XM_031890592.1
Accession Version	XM_031890592.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	centrosomal protein of 170 kDa protein B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTCA CATCATGGTT CCTTGTCAGC AGCTCTGGCA CGCGCCATCG GCTGCCACGG 
GAAATGATCT TTGTTGGTCG GGAGGATTGT GAGCTTATGC TTCAGTCCCG CAGTGTGGAT 
AAGCAGCATG CAGTAATCAA CTATGACAGT GACAAAGATG AACATCGCGT CAAGGACCTT 
GGGAGCTTAA ATGGGACCTT TGTAAATGAT GTAAGGATTC CTGACCAGAA GTACATTACT 
CTGAAGCTGA GTGACAACAT ACGTTTTGGC TACGATATTA ATACGTATGT ACTGGAGCAG 
ATCCAGCACC AGGTACCAGA AGAGGCACTG AAGTACTGTG GCCTTCCAGT TACTGCTTTT 
GAACAATTTC CTTCATCTTC TGGCAAACAT GAAAAGTACA CCAGCCACCT TCAGATGTGT 
TTGAAAACCG CTGCAGCAGG AAGAGAAGAC CAGTTTAAAG AGCATGGTGC CCACGTAGAC 
TCTGCCCAAG CCAAACAGGA CAAAGCTGAC AAGAAAGCTA CTAGTGATAT TCCTGCCTAC 
CGTACACCTT TGTACGGTCA ACCATCTTGG TGGGGAGAGG ATGATGATAA CAAGCTTGAT 
AAGGAAGGCA GAAGACAAGA TGAACACTAT TCAGAAAGGC CAAACGATAT GACTCAACAT 
GAGGAAGAAA TAAATGGTAA TATGAGCTAC AGGGATTCTC AGGATCAGTG TGTGTACCCA 
TTCAGAAGAG AACCTAGTTA CTTTGAAATT CCAACTAAAG ACTTTCAACA GCCTGTAAAG 
CCCCCAGAAA CCCAGGTCTA TGAAATCCCT ACTAAGGACA CAGATGCAGT GCCCCCTGTA 
ACACCCCCTG TCATGCAGAG CCATGCCTCT TTTACAATTG AGTTTGATGA TTGCCAGCCT 
GGAAAAATAA AAATTAAAGA TCATGTAACA AAATTTTCTA TGAGACAGAG GAAAACAGCT 
GGAAAGGAAC CTGCTCCAAC AGAAATGGTG TCAGCAGAAA GTAAGGTGGC TGACTGGCTG 
GTTCAAAATG ACCCAAGTTT GATTAGAAGA CCAGCTCCTG GAGAAGATGT CTACAGTACC 
AAGAGTGACC TTCCTATCCA TAACAGAACT CTTAAAGGTA ACAGACATGA AGATGGAACA 
CAGAGTGATT CAGAAGACCC CTTGGTGGCC CAACCTGAAC AGGAAATTGG AACACCTGAT 
CACCCACAGC TCCAGCGGCA GATCAAGCGC GAACCTGAAG AATTGCTGCA CAATCAGCAG 
GCGTTTGTCA TACAGTTTTT TGATGATGAT GATGCCCCTA GGAAAAAACG GTCCCAGTCT 
TTTACACACA ATGCAAACTC CCCTCAGAAT GACACAGACC CAGTCCTTAA AGCAAAAGCT 
GAAAAGCGAA AGGGCACATT GCATGTTGAA AAAGTGTCAA CTAATGGAAT GGGTTCAACT 
GCTCCAGCAT CAAAATCACT GTCAAGCCCT TCATTCCCTC AAAGATCAAA CTCTTTTAGG 
CGAGAAAAGA CAGAGGACCG CATTTCTTCT GCACCTACGA CAGCAAAGTT ACCAGGGAAG 
AACTATGGAA GTGTTGGGAA GAAATCAAAG CTTGCCCAAG AATTTGCTGC TGAATATATG 
CGTGAACAAG TAGAGGTAGT TAAACAGGCA GCAGAAAAAC CCATGACCTT GCCTTTGTCT 
ACATCAATAT TGCAGCAACC AGCCTCACAA GTGCAAATCT CAAGCCAAGC ACAAACAATG 
CAGCTAACAT CTGATGTAAG AACAGGAAAA GTTAAAAATG AAGAAGAGGA CAACCTAAGT 
GATGCAGGAA CATACACAAT AGAAACAGAG ACACAGGATA GAGAAGTTGA ACAAGCCAGG 
AAAATGATAG ACCAGGTCTT TGGAGTATTT GAATCCGATG AGTATTCCAA GATTGCATCC 
ACAGTTTATA GGCCAGTTAT TAAATTGGGA GAAGAGGAAC CTCTTGCCAG TGAACCAAGT 
GTACCACACA AGCCCATAAT GTCATCAACT CCACCAGTAA AACTAAGTAA TGGGCTTCCG 
GCTGAAACTT TGATTGAAAG AACTGGGTCA AGCTCCAAAC AAAGTCAGAA ATGGGTTTCC 
CGTTGGGCAA GCCTCGCTGA CAGTTATCCC GATGCATCAC CAACATCTCC TTTGGACAGT 
CAGAAGCAAG GCTTAATTGC AGATGATGAA GACGTTATTG AGCGTACAGA GCACCAAGGA 
GAAGCAGATC CCACTGTGCC ATCAAGAACA AGACGGCTCC TTCCACAACT TCCTCCTGAA 
AATGTAATGC CAACAATCTT TGTATGCCAA GAGTCTTTTT CAGATGAATC TCAAAGGAAG 
TCTTTGGAAG AACCAGAAAA GAGAATTTCT GAAGAAAACA GCAGTTTATT ATTAGTTCAA 
GAGGAATTAG ATCCAGACAG TCTTAGTGAT ACCAGCAAGT CTGATGATGG TGTCATATCA 
GTCAGGAAGT CTGCCAAGGC TTCCAACACT TATAGAAATG GATGGAAGGG AGAGGAGTCT 
CATAGTAGGG AACCATCTGT TCAGAGAACT TCAGTACCTT CTAATGAAAA GAAATCAACT 
TGTTTTTATG TTGGAAATGA TGACGTTGGA AGTGTAAAAC AAACAAGCTT TGGATTGTCC 
TCTAAAGATG TGATTAAGGG CCCAGAAAGC CACATTAAGA TGAAAGTTAA TTTACACATA 
ACTGCTGAGA CTCCAAGCTC AGGAAAGGCA GTTAATCAGT TTCCCAAGAA AGATAATGGC 
TCTGCAAAAG ATCCTCTTTC ATTTGTAAGG CAGGAAAGCT TTACCAAAGA GACATCCAGT 
AGCAATGTTT TACCAAATAA GCTTCCACAT ATTTCAACTC ATCCATTGCT TAAAGATTTA 
AGTGTTACAA AATCAAATCA TGACTACAGT AAGGAGACAC GCCTAATACT TAAAGAGACA 
GAAACCGCTT TGGCTGCTTT AGAAGCTAAG CTCTTTACAC AGTCCCACCT TGATGAAATA 
GAAAATACTC CTTGTTTGCG TGATGACTCC TTATCAGGAG ACTCTGATGT GGATACAGCC 
AGCACAGTTA GTCTAGTCAG TGATAAAAAT GTACCAAGTC ATTCACAGAA AAACAGAATT 
GTCAGCCTTC AGAAAGAAAA GTCTTCCTCC ACTTCTTCTA TACAAGAGCA ATATTGTCAA 
CCAAGTGCTC GAGAACGTCT TTCTGAGAAA CGAAGGACAG TTCCTGCTGA TGCTGGAACT 
AGAAATGTAA CCAAACGACT AGGGATGACA CGTAGCACTG GAGCACGTGG CTCATTAGAC 
TTCACAGATG AAGAAAGGTG TTCTAGTCTT CCTTATATGC CAGTTTCAGA AACTGTTTCA 
TCTGATTATG AACATTCTTC TTCAAGGCAT ATTTCAAGGA GGAAACCCTT TGGTCAGACT 
TGCAAAGATG AATCGAGTAG ATCATCAAAT GCACAAAAAG TTCAGCAGGC TCTTACACGC 
TCTAACAGTT TATCAACTCC GAGGCCTACA AGAGCTTCCA AACTGCGTCG CGCTCGTCTC 
GGCGAATCTT CTGACAATGA AAGTGCTGAT GTAGAAAGAT CAAATATAAG TCCAGGAACG 
TCTTCTGCAA ACTCATCTTC TGCTAAAAGT TCTACAGAAA CTAAAAAGCC ATCTCGTTTA 
GATATCCTTG CTATGCCAAG AAAACGAGCA GGTTCATTTA ATGTTCCAAG TGACTCTGAA 
ACAACTTCTT CAGTAAGAGG AATGTTTTCA GGACGTAGCA TAGACCAAAG TTACACTAGT 
AGAAAACCAG CTGTGGCAGA AAGCAAGCAG CCTCCCAAGA AACCTCTACC ACCTACACAG 
AAGCAAACAC CAAGACCTCG CTCAAGCAGT GTCAAGTACT CATCATCTTC TACTTCACGA 
AGGAGGCAAC AGGGATCAGA TTATGTTTCT ACATCTGAGG AGGAGTGTGG CTCAAATCAC 
AGCACTCCTA AACACAAACA CTCCCGTGCT TCAACAGCCA CTCAGACTTC AAGATCAAGC 
AGTGTCTCTC GACGTCAGAT GCCCAACAGT CGCCAAGATG AGGAAGAGGA TGAGCAGGAA 
GTTTATAACA ACTTCATGGC CCAGTCTGTG GAAATTGCAG AAATAGCCAG ATTGAGTCAA 
ACCCTGGTGA AAGATGTTGC CAGTTTGGCA AGAGAGATTC ATGATGTAGC AGGAGATGGA 
GATTCTCAGA GTTCCTCAGG AACAGGACAG AGCACCTCAA TCAGTTCTGT GCCAAACACC 
CCAGCCTCAA CCATTTCAGC AAGGGAAGAG ATAATTAAAC GGTCCTTACA ACGAACGTGC 
TCATCACAGT TGGTGCATCA TATACCAGAA GCAAGCCTGA ACTACCAGAA GATTCCTCCA 
GGTTCAACAG GCCTTGAAGA CTTTGATCAG AATATGAATG ACAGTCGAGA AGATCCATCA 
AAGAGAAGAG CTAGGAACAT AGAAGAGGTG ATTTTTGACA ATCTTATGCT GAATCCTGTG 
TCTCATTTAT CACATACAAT ATGCGCAAAC ACTGAGGTCC TTACAGAAAA AATGAAAATT 
TTATTTCAGA ATACAGAGAA GAACTGGGAA GAAATTGAAG CAAAGATTAA CTCTGAAAAC 
GAAGTGCCAA TTCTTAAAAC ATCCAATAAG GAAATCAGCT CTATCTTGAA AGAGTTGAGA 
AGGGTTCAGA AACAATTGGA AGTTATAAAT GCAATCATCG ACCGCTCTGG ACATCTAGAT 
GTTCCGTCAA GTAATAAAAA GACATCTTCG ACTATCCTGA CTTCAAATCC TTTATCTAGG 
ACTACTAACA ATTCAGCTGC CAGAACAGAG TCTCAAACTC CTGGCCATGT GAGAAATTAC 
ATGCATAAAT CCAGCTCCAG CTCCTCAAGA TCCCCAGGAT CCAGTTTCAG TCGGGATGAC 
GAAGAAACAT ATATTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746452.1
CDS	248..5188
Translation	MSVTSWFLVSSSGTRHRLPREMIFVGREDCELMLQSRSVDKQHAVINYDSDKDEHRVKDLGSLNGTFVNDVRIPDQKYITLKLSDNIRFGYDINTYVLEQIQHQVPEEALKYCGLPVTAFEQFPSSSGKHEKYTSHLQMCLKTAAAGREDQFKEHGAHVDSAQAKQDKADKKATSDIPAYRTPLYGQPSWWGEDDDNKLDKEGRRQDEHYSERPNDMTQHEEEINGNMSYRDSQDQCVYPFRREPSYFEIPTKDFQQPVKPPETQVYEIPTKDTDAVPPVTPPVMQSHASFTIEFDDCQPGKIKIKDHVTKFSMRQRKTAGKEPAPTEMVSAESKVADWLVQNDPSLIRRPAPGEDVYSTKSDLPIHNRTLKGNRHEDGTQSDSEDPLVAQPEQEIGTPDHPQLQRQIKREPEELLHNQQAFVIQFFDDDDAPRKKRSQSFTHNANSPQNDTDPVLKAKAEKRKGTLHVEKVSTNGMGSTAPASKSLSSPSFPQRSNSFRREKTEDRISSAPTTAKLPGKNYGSVGKKSKLAQEFAAEYMREQVEVVKQAAEKPMTLPLSTSILQQPASQVQISSQAQTMQLTSDVRTGKVKNEEEDNLSDAGTYTIETETQDREVEQARKMIDQVFGVFESDEYSKIASTVYRPVIKLGEEEPLASEPSVPHKPIMSSTPPVKLSNGLPAETLIERTGSSSKQSQKWVSRWASLADSYPDASPTSPLDSQKQGLIADDEDVIERTEHQGEADPTVPSRTRRLLPQLPPENVMPTIFVCQESFSDESQRKSLEEPEKRISEENSSLLLVQEELDPDSLSDTSKSDDGVISVRKSAKASNTYRNGWKGEESHSREPSVQRTSVPSNEKKSTCFYVGNDDVGSVKQTSFGLSSKDVIKGPESHIKMKVNLHITAETPSSGKAVNQFPKKDNGSAKDPLSFVRQESFTKETSSSNVLPNKLPHISTHPLLKDLSVTKSNHDYSKETRLILKETETALAALEAKLFTQSHLDEIENTPCLRDDSLSGDSDVDTASTVSLVSDKNVPSHSQKNRIVSLQKEKSSSTSSIQEQYCQPSARERLSEKRRTVPADAGTRNVTKRLGMTRSTGARGSLDFTDEERCSSLPYMPVSETVSSDYEHSSSRHISRRKPFGQTCKDESSRSSNAQKVQQALTRSNSLSTPRPTRASKLRRARLGESSDNESADVERSNISPGTSSANSSSAKSSTETKKPSRLDILAMPRKRAGSFNVPSDSETTSSVRGMFSGRSIDQSYTSRKPAVAESKQPPKKPLPPTQKQTPRPRSSSVKYSSSSTSRRRQQGSDYVSTSEEECGSNHSTPKHKHSRASTATQTSRSSSVSRRQMPNSRQDEEEDEQEVYNNFMAQSVEIAEIARLSQTLVKDVASLAREIHDVAGDGDSQSSSGTGQSTSISSVPNTPASTISAREEIIKRSLQRTCSSQLVHHIPEASLNYQKIPPGSTGLEDFDQNMNDSREDPSKRRARNIEEVIFDNLMLNPVSHLSHTICANTEVLTEKMKILFQNTEKNWEEIEAKINSENEVPILKTSNKEISSILKELRRVQKQLEVINAIIDRSGHLDVPSSNKKTSSTILTSNPLSRTTNNSAARTESQTPGHVRNYMHKSSSSSSRSPGSSFSRDDEETYIV

Target ORF information:

RefSeq Version	XM_031890592.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis centrosomal protein 170B (cep170b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890592.1

ORF Insert Sequence:

ATGAGTGTCA CATCATGGTT CCTTGTCAGC AGCTCTGGCA CGCGCCATCG GCTGCCACGG 
GAAATGATCT TTGTTGGTCG GGAGGATTGT GAGCTTATGC TTCAGTCCCG CAGTGTGGAT 
AAGCAGCATG CAGTAATCAA CTATGACAGT GACAAAGATG AACATCGCGT CAAGGACCTT 
GGGAGCTTAA ATGGGACCTT TGTAAATGAT GTAAGGATTC CTGACCAGAA GTACATTACT 
CTGAAGCTGA GTGACAACAT ACGTTTTGGC TACGATATTA ATACGTATGT ACTGGAGCAG 
ATCCAGCACC AGGTACCAGA AGAGGCACTG AAGTACTGTG GCCTTCCAGT TACTGCTTTT 
GAACAATTTC CTTCATCTTC TGGCAAACAT GAAAAGTACA CCAGCCACCT TCAGATGTGT 
TTGAAAACCG CTGCAGCAGG AAGAGAAGAC CAGTTTAAAG AGCATGGTGC CCACGTAGAC 
TCTGCCCAAG CCAAACAGGA CAAAGCTGAC AAGAAAGCTA CTAGTGATAT TCCTGCCTAC 
CGTACACCTT TGTACGGTCA ACCATCTTGG TGGGGAGAGG ATGATGATAA CAAGCTTGAT 
AAGGAAGGCA GAAGACAAGA TGAACACTAT TCAGAAAGGC CAAACGATAT GACTCAACAT 
GAGGAAGAAA TAAATGGTAA TATGAGCTAC AGGGATTCTC AGGATCAGTG TGTGTACCCA 
TTCAGAAGAG AACCTAGTTA CTTTGAAATT CCAACTAAAG ACTTTCAACA GCCTGTAAAG 
CCCCCAGAAA CCCAGGTCTA TGAAATCCCT ACTAAGGACA CAGATGCAGT GCCCCCTGTA 
ACACCCCCTG TCATGCAGAG CCATGCCTCT TTTACAATTG AGTTTGATGA TTGCCAGCCT 
GGAAAAATAA AAATTAAAGA TCATGTAACA AAATTTTCTA TGAGACAGAG GAAAACAGCT 
GGAAAGGAAC CTGCTCCAAC AGAAATGGTG TCAGCAGAAA GTAAGGTGGC TGACTGGCTG 
GTTCAAAATG ACCCAAGTTT GATTAGAAGA CCAGCTCCTG GAGAAGATGT CTACAGTACC 
AAGAGTGACC TTCCTATCCA TAACAGAACT CTTAAAGGTA ACAGACATGA AGATGGAACA 
CAGAGTGATT CAGAAGACCC CTTGGTGGCC CAACCTGAAC AGGAAATTGG AACACCTGAT 
CACCCACAGC TCCAGCGGCA GATCAAGCGC GAACCTGAAG AATTGCTGCA CAATCAGCAG 
GCGTTTGTCA TACAGTTTTT TGATGATGAT GATGCCCCTA GGAAAAAACG GTCCCAGTCT 
TTTACACACA ATGCAAACTC CCCTCAGAAT GACACAGACC CAGTCCTTAA AGCAAAAGCT 
GAAAAGCGAA AGGGCACATT GCATGTTGAA AAAGTGTCAA CTAATGGAAT GGGTTCAACT 
GCTCCAGCAT CAAAATCACT GTCAAGCCCT TCATTCCCTC AAAGATCAAA CTCTTTTAGG 
CGAGAAAAGA CAGAGGACCG CATTTCTTCT GCACCTACGA CAGCAAAGTT ACCAGGGAAG 
AACTATGGAA GTGTTGGGAA GAAATCAAAG CTTGCCCAAG AATTTGCTGC TGAATATATG 
CGTGAACAAG TAGAGGTAGT TAAACAGGCA GCAGAAAAAC CCATGACCTT GCCTTTGTCT 
ACATCAATAT TGCAGCAACC AGCCTCACAA GTGCAAATCT CAAGCCAAGC ACAAACAATG 
CAGCTAACAT CTGATGTAAG AACAGGAAAA GTTAAAAATG AAGAAGAGGA CAACCTAAGT 
GATGCAGGAA CATACACAAT AGAAACAGAG ACACAGGATA GAGAAGTTGA ACAAGCCAGG 
AAAATGATAG ACCAGGTCTT TGGAGTATTT GAATCCGATG AGTATTCCAA GATTGCATCC 
ACAGTTTATA GGCCAGTTAT TAAATTGGGA GAAGAGGAAC CTCTTGCCAG TGAACCAAGT 
GTACCACACA AGCCCATAAT GTCATCAACT CCACCAGTAA AACTAAGTAA TGGGCTTCCG 
GCTGAAACTT TGATTGAAAG AACTGGGTCA AGCTCCAAAC AAAGTCAGAA ATGGGTTTCC 
CGTTGGGCAA GCCTCGCTGA CAGTTATCCC GATGCATCAC CAACATCTCC TTTGGACAGT 
CAGAAGCAAG GCTTAATTGC AGATGATGAA GACGTTATTG AGCGTACAGA GCACCAAGGA 
GAAGCAGATC CCACTGTGCC ATCAAGAACA AGACGGCTCC TTCCACAACT TCCTCCTGAA 
AATGTAATGC CAACAATCTT TGTATGCCAA GAGTCTTTTT CAGATGAATC TCAAAGGAAG 
TCTTTGGAAG AACCAGAAAA GAGAATTTCT GAAGAAAACA GCAGTTTATT ATTAGTTCAA 
GAGGAATTAG ATCCAGACAG TCTTAGTGAT ACCAGCAAGT CTGATGATGG TGTCATATCA 
GTCAGGAAGT CTGCCAAGGC TTCCAACACT TATAGAAATG GATGGAAGGG AGAGGAGTCT 
CATAGTAGGG AACCATCTGT TCAGAGAACT TCAGTACCTT CTAATGAAAA GAAATCAACT 
TGTTTTTATG TTGGAAATGA TGACGTTGGA AGTGTAAAAC AAACAAGCTT TGGATTGTCC 
TCTAAAGATG TGATTAAGGG CCCAGAAAGC CACATTAAGA TGAAAGTTAA TTTACACATA 
ACTGCTGAGA CTCCAAGCTC AGGAAAGGCA GTTAATCAGT TTCCCAAGAA AGATAATGGC 
TCTGCAAAAG ATCCTCTTTC ATTTGTAAGG CAGGAAAGCT TTACCAAAGA GACATCCAGT 
AGCAATGTTT TACCAAATAA GCTTCCACAT ATTTCAACTC ATCCATTGCT TAAAGATTTA 
AGTGTTACAA AATCAAATCA TGACTACAGT AAGGAGACAC GCCTAATACT TAAAGAGACA 
GAAACCGCTT TGGCTGCTTT AGAAGCTAAG CTCTTTACAC AGTCCCACCT TGATGAAATA 
GAAAATACTC CTTGTTTGCG TGATGACTCC TTATCAGGAG ACTCTGATGT GGATACAGCC 
AGCACAGTTA GTCTAGTCAG TGATAAAAAT GTACCAAGTC ATTCACAGAA AAACAGAATT 
GTCAGCCTTC AGAAAGAAAA GTCTTCCTCC ACTTCTTCTA TACAAGAGCA ATATTGTCAA 
CCAAGTGCTC GAGAACGTCT TTCTGAGAAA CGAAGGACAG TTCCTGCTGA TGCTGGAACT 
AGAAATGTAA CCAAACGACT AGGGATGACA CGTAGCACTG GAGCACGTGG CTCATTAGAC 
TTCACAGATG AAGAAAGGTG TTCTAGTCTT CCTTATATGC CAGTTTCAGA AACTGTTTCA 
TCTGATTATG AACATTCTTC TTCAAGGCAT ATTTCAAGGA GGAAACCCTT TGGTCAGACT 
TGCAAAGATG AATCGAGTAG ATCATCAAAT GCACAAAAAG TTCAGCAGGC TCTTACACGC 
TCTAACAGTT TATCAACTCC GAGGCCTACA AGAGCTTCCA AACTGCGTCG CGCTCGTCTC 
GGCGAATCTT CTGACAATGA AAGTGCTGAT GTAGAAAGAT CAAATATAAG TCCAGGAACG 
TCTTCTGCAA ACTCATCTTC TGCTAAAAGT TCTACAGAAA CTAAAAAGCC ATCTCGTTTA 
GATATCCTTG CTATGCCAAG AAAACGAGCA GGTTCATTTA ATGTTCCAAG TGACTCTGAA 
ACAACTTCTT CAGTAAGAGG AATGTTTTCA GGACGTAGCA TAGACCAAAG TTACACTAGT 
AGAAAACCAG CTGTGGCAGA AAGCAAGCAG CCTCCCAAGA AACCTCTACC ACCTACACAG 
AAGCAAACAC CAAGACCTCG CTCAAGCAGT GTCAAGTACT CATCATCTTC TACTTCACGA 
AGGAGGCAAC AGGGATCAGA TTATGTTTCT ACATCTGAGG AGGAGTGTGG CTCAAATCAC 
AGCACTCCTA AACACAAACA CTCCCGTGCT TCAACAGCCA CTCAGACTTC AAGATCAAGC 
AGTGTCTCTC GACGTCAGAT GCCCAACAGT CGCCAAGATG AGGAAGAGGA TGAGCAGGAA 
GTTTATAACA ACTTCATGGC CCAGTCTGTG GAAATTGCAG AAATAGCCAG ATTGAGTCAA 
ACCCTGGTGA AAGATGTTGC CAGTTTGGCA AGAGAGATTC ATGATGTAGC AGGAGATGGA 
GATTCTCAGA GTTCCTCAGG AACAGGACAG AGCACCTCAA TCAGTTCTGT GCCAAACACC 
CCAGCCTCAA CCATTTCAGC AAGGGAAGAG ATAATTAAAC GGTCCTTACA ACGAACGTGC 
TCATCACAGT TGGTGCATCA TATACCAGAA GCAAGCCTGA ACTACCAGAA GATTCCTCCA 
GGTTCAACAG GCCTTGAAGA CTTTGATCAG AATATGAATG ACAGTCGAGA AGATCCATCA 
AAGAGAAGAG CTAGGAACAT AGAAGAGGTG ATTTTTGACA ATCTTATGCT GAATCCTGTG 
TCTCATTTAT CACATACAAT ATGCGCAAAC ACTGAGGTCC TTACAGAAAA AATGAAAATT 
TTATTTCAGA ATACAGAGAA GAACTGGGAA GAAATTGAAG CAAAGATTAA CTCTGAAAAC 
GAAGTGCCAA TTCTTAAAAC ATCCAATAAG GAAATCAGCT CTATCTTGAA AGAGTTGAGA 
AGGGTTCAGA AACAATTGGA AGTTATAAAT GCAATCATCG ACCGCTCTGG ACATCTAGAT 
GTTCCGTCAA GTAATAAAAA GACATCTTCG ACTATCCTGA CTTCAAATCC TTTATCTAGG 
ACTACTAACA ATTCAGCTGC CAGAACAGAG TCTCAAACTC CTGGCCATGT GAGAAATTAC 
ATGCATAAAT CCAGCTCCAG CTCCTCAAGA TCCCCAGGAT CCAGTTTCAG TCGGGATGAC 
GAAGAAACAT ATATTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep170b ( XM_031890593.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746453.1 Xenopus tropicalis centrosomal protein 170B (cep170b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa63817
Clone ID Related Accession (Same CDS sequence)	XM_031890593.1
Accession Version	XM_031890593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	centrosomal protein of 170 kDa protein B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTCA CATCATGGTT CCTTGTCAGC AGCTCTGGCA CGCGCCATCG GCTGCCACGG 
GAAATGATCT TTGTTGGTCG GGAGGATTGT GAGCTTATGC TTCAGTCCCG CAGTGTGGAT 
AAGCAGCATG CAGTAATCAA CTATGACAGT GACAAAGATG AACATCGCGT CAAGGACCTT 
GGGAGCTTAA ATGGGACCTT TGTAAATGAT GTAAGGATTC CTGACCAGAA GTACATTACT 
CTGAAGCTGA GTGACAACAT ACGTTTTGGC TACGATATTA ATACGTATGT ACTGGAGCAG 
ATCCAGCACC AGGTACCAGA AGAGGCACTG AAGTACTGTG GCCTTCCAGT TACTGCTTTT 
GAACAATTTC CTTCATCTTC TGGCAAACAT GAAAAGTACA CCAGCCACCT TCAGATGTGT 
TTGAAAACCG CTGCAGCAGG AAGAGAAGAC CAGTTTAAAG AGCATGGTGC CCACGTAGAC 
TCTGCCCAAG CCAAACAGGA CAAAGCTGAC AAGAAAGCTA CTAGTGATAT TCCTGCCTAC 
CGTACACCTT TGTACGGTCA ACCATCTTGG TGGGGAGAGG ATGATGATAA CAAGCTTGAT 
AAGGAAGGCA GAAGACAAGA TGAACACTAT TCAGAAAGGC CAAACGATAT GACTCAACAT 
GAGGAAGAAA TAAATGGTAA TATGAGCTAC AGGGATTCTC AGGATCAGTG TGTGTACCCA 
TTCAGAAGAG AACCTAGTTA CTTTGAAATT CCAACTAAAG ACTTTCAACA GCCTGTAAAG 
CCCCCAGAAA CCCAGGTCTA TGAAATCCCT ACTAAGGACA CAGATGCAGT GCCCCCTGTA 
ACACCCCCTG TCATGCAGAG CCATGCCTCT TTTACAATTG AGTTTGATGA TTGCCAGCCT 
GGAAAAATAA AAATTAAAGA TCATGTAACA AAATTTTCTA TGAGACAGAG GAAAACAGCT 
GGAAAGGAAC CTGCTCCAAC AGAAATGGTG TCAGCAGAAA GTAAGGTGGC TGACTGGCTG 
GTTCAAAATG ACCCAAGTTT GATTAGAAGA CCAGCTCCTG GAGAAGATGT CTACAGTACC 
AAGAGTGACC TTCCTATCCA TAACAGAACT CTTAAAGGTA ACAGACATGA AGATGGAACA 
CAGAGTGATT CAGAAGACCC CTTGGTGGCC CAACCTGAAC AGGAAATTGG AACACCTGAT 
CACCCACAGC TCCAGCGGCA GATCAAGCGC GAACCTGAAG AATTGCTGCA CAATCAGCAG 
GCGTTTGTCA TACAGTTTTT TGATGATGAT GATGCCCCTA GGAAAAAACG GTCCCAGTCT 
TTTACACACA ATGCAAACTC CCCTCAGAAT GACACAGACC CAGTCCTTAA AGCAAAAGCT 
GAAAAGCGAA AGGGCACATT GCATGTTGAA AAAGTGTCAA CTAATGGAAT GGGTTCAACT 
GCTCCAGCAT CAAAATCACT GTCAAGCCCT TCATTCCCTC AAAGATCAAA CTCTTTTAGG 
CGAGAAAAGA CAGAGGACCG CATTTCTTCT GCACCTACGA CAGCAAAGTT ACCAGGGAAG 
AACTATGGAA GTGTTGGGAA GAAATCAAAG CTTGCCCAAG AATTTGCTGC TGAATATATG 
CGTGAACAAG TAGAGGTAGT TAAACAGGCA GCAGAAAAAC CCATGACCTT GCCTTTGTCT 
ACATCAATAT TGCAGCAACC AGCCTCACAA GTGCAAATCT CAAGCCAAGC ACAAACAATG 
CAGCTAACAT CTGATGTAAG AACAGGAAAA GTTAAAAATG AAGAAGAGGA CAACCTAAGT 
GATGCAGGAA CATACACAAT AGAAACAGAG ACACAGGATA GAGAAGTTGA ACAAGCCAGG 
AAAATGATAG ACCAGGTCTT TGGAGTATTT GAATCCGATG AGTATTCCAA GATTGCATCC 
ACAGTTTATA GGCCAGTTAT TAAATTGGGA GAAGAGGAAC CTCTTGCCAG TGAACCAAGT 
GTACCACACA AGCCCATAAT GTCATCAACT CCACCAGTAA AACTAAGTAA TGGGCTTCCG 
GCTGAAACTT TGATTGAAAG AACTGGGTCA AGCTCCAAAC AAAGTCAGAA ATGGGTTTCC 
CGTTGGGCAA GCCTCGCTGA CAGTTATCCC GATGCATCAC CAACATCTCC TTTGGACAGT 
CAGAAGCAAG GCTTAATTGC AGATGATGAA GACGTTATTG AGCGTACAGA GCACCAAGGA 
GAAGCAGATC CCACTGTGCC ATCAAGAACA AGACGGCTCC TTCCACAACT TCCTCCTGAA 
AATGTAATGC CAACAATCTT TGTATGCCAA GAGTCTTTTT CAGATGAATC TCAAAGGAAG 
TCTTTGGAAG AACCAGAAAA GAGAATTTCT GAAGAAAACA GCAGTTTATT ATTAGTTCAA 
GAGGAATTAG ATCCAGACAG TCTTAGTGAT ACCAGCAAGT CTGATGATGG TGTCATATCA 
GTCAGGAAGT CTGCCAAGGC TTCCAACACT TATAGAAATG GATGGAAGGG AGAGGAGTCT 
CATAGTAGGG AACCATCTGT TCAGAGAACT TCAGTACCTT CTAATGAAAA GAAATCAACT 
TGTTTTTATG TTGGAAATGA TGACGTTGGA AGTGTAAAAC AAACAAGCTT TGGATTGTCC 
TCTAAAGATG TGATTAAGGG CCCAGAAAGC CACATTAAGA TGAAAGTTAA TTTACACATA 
ACTGCTGAGA CTCCAAGCTC AGGAAAGGCA GTTAATCAGT TTCCCAAGAA AGATAATGGC 
TCTGCAAAAG ATCCTCTTTC ATTTGTAAGG CAGGAAAGCT TTACCAAAGA GACATCCAGT 
AGCAATGTTT TACCAAATAA GCTTCCACAT ATTTCAACTC ATCCATTGCT TAAAGATTTA 
AGTGTTACAA AATCAAATCA TGACTACAGT AAGGAGACAC GCCTAATACT TAAAGAGACA 
GAAACCGCTT TGGCTGCTTT AGAAGCTAAG CTCTTTACAC AGTCCCACCT TGATGAAATA 
GAAAATACTC CTTGTTTGCG TGATGACTCC TTATCAGGAG ACTCTGATGT GGATACAGCC 
AGCACAGTTA GTCTAGTCAG TGATAAAAAT GTACCAAGTC ATTCACAGAA AAACAGAATT 
GTCAGCCTTC AGAAAGAAAA GTCTTCCTCC ACTTCTTCTA TACAAGAGCA ATATTGTCAA 
CCAAGTGCTC GAGAACGTCT TTCTGAGAAA CGAAGGACAG TTCCTGCTGA TGCTGGAACT 
AGAAATGTAA CCAAACGACT AGGGATGACA CGTAGCACTG GAGCACGTGG CTCATTAGAC 
TTCACAGATG AAGAAAGGTG TTCTAGTCTT CCTTATATGC CAGTTTCAGA AACTGTTTCA 
TCTGATTATG AACATTCTTC TTCAAGGCAT ATTTCAAGGA GGAAACCCTT TGGTCAGACT 
TGCAAAGATG AATCGAGTAG ATCATCAAAT GCACAAAAAG TTCAGCAGGC TCTTACACGC 
TCTAACAGTT TATCAACTCC GAGGCCTACA AGAGCTTCCA AACTGCGTCG CGCTCGTCTC 
GGCGAATCTT CTGACAATGA AAGTGCTGAT GTAGAAAGAT CAAATATAAG TCCAGGAACG 
TCTTCTGCAA ACTCATCTTC TGCTAAAAGT TCTACAGAAA CTAAAAAGCC ATCTCGTTTA 
GATATCCTTG CTATGCCAAG AAAACGAGCA GGTTCATTTA ATGTTCCAAG TGACTCTGAA 
ACAACTTCTT CAGTAAGAGG AATGTTTTCA GGACGTAGCA TAGACCAAAG TTACACTAGT 
AGAAAACCAG CTGTGGCAGA AAGCAAGCAG CCTCCCAAGA AACCTCTACC ACCTACACAG 
AAGCAAACAC CAAGACCTCG CTCAAGCAGT GTCAAGTACT CATCATCTTC TACTTCACGA 
AGGAGGCAAC AGGGATCAGA TTATGTTTCT ACATCTGAGG AGGAGTGTGG CTCAAATCAC 
AGCACTCCTA AACACAAACA CTCCCGTGCT TCAACAGCCA CTCAGACTTC AAGATCAAGC 
AGTGTCTCTC GACGTCAGAT GCCCAACAGT CGCCAAGATG AGGAAGAGGA TGAGCAGGAA 
GTTTATAACA ACTTCATGGC CCAGTCTGTG GAAATTGCAG AAATAGCCAG ATTGAGTCAA 
ACCCTGGTGA AAGATGTTGC CAGTTTGGCA AGAGAGATTC ATGATGTAGC AGGAGATGGA 
GATTCTCAGA GTTCCTCAGG AACAGGACAG AGCACCTCAA TCAGTTCTGT GCCAAACACC 
CCAGCCTCAA CCATTTCAGC AAGGGAAGAG TTGGTGCATC ATATACCAGA AGCAAGCCTG 
AACTACCAGA AGATTCCTCC AGGTTCAACA GGCCTTGAAG ACTTTGATCA GAATATGAAT 
GACAGTCGAG AAGATCCATC AAAGAGAAGA GCTAGGAACA TAGAAGAGGT GATTTTTGAC 
AATCTTATGC TGAATCCTGT GTCTCATTTA TCACATACAA TATGCGCAAA CACTGAGGTC 
CTTACAGAAA AAATGAAAAT TTTATTTCAG AATACAGAGA AGAACTGGGA AGAAATTGAA 
GCAAAGATTA ACTCTGAAAA CGAAGTGCCA ATTCTTAAAA CATCCAATAA GGAAATCAGC 
TCTATCTTGA AAGAGTTGAG AAGGGTTCAG AAACAATTGG AAGTTATAAA TGCAATCATC 
GACCGCTCTG GACATCTAGA TGTTCCGTCA AGTAATAAAA AGACATCTTC GACTATCCTG 
ACTTCAAATC CTTTATCTAG GACTACTAAC AATTCAGCTG CCAGAACAGA GTCTCAAACT 
CCTGGCCATG TGAGAAATTA CATGCATAAA TCCAGCTCCA GCTCCTCAAG ATCCCCAGGA 
TCCAGTTTCA GTCGGGATGA CGAAGAAACA TATATTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746453.1
CDS	249..5150
Translation	MSVTSWFLVSSSGTRHRLPREMIFVGREDCELMLQSRSVDKQHAVINYDSDKDEHRVKDLGSLNGTFVNDVRIPDQKYITLKLSDNIRFGYDINTYVLEQIQHQVPEEALKYCGLPVTAFEQFPSSSGKHEKYTSHLQMCLKTAAAGREDQFKEHGAHVDSAQAKQDKADKKATSDIPAYRTPLYGQPSWWGEDDDNKLDKEGRRQDEHYSERPNDMTQHEEEINGNMSYRDSQDQCVYPFRREPSYFEIPTKDFQQPVKPPETQVYEIPTKDTDAVPPVTPPVMQSHASFTIEFDDCQPGKIKIKDHVTKFSMRQRKTAGKEPAPTEMVSAESKVADWLVQNDPSLIRRPAPGEDVYSTKSDLPIHNRTLKGNRHEDGTQSDSEDPLVAQPEQEIGTPDHPQLQRQIKREPEELLHNQQAFVIQFFDDDDAPRKKRSQSFTHNANSPQNDTDPVLKAKAEKRKGTLHVEKVSTNGMGSTAPASKSLSSPSFPQRSNSFRREKTEDRISSAPTTAKLPGKNYGSVGKKSKLAQEFAAEYMREQVEVVKQAAEKPMTLPLSTSILQQPASQVQISSQAQTMQLTSDVRTGKVKNEEEDNLSDAGTYTIETETQDREVEQARKMIDQVFGVFESDEYSKIASTVYRPVIKLGEEEPLASEPSVPHKPIMSSTPPVKLSNGLPAETLIERTGSSSKQSQKWVSRWASLADSYPDASPTSPLDSQKQGLIADDEDVIERTEHQGEADPTVPSRTRRLLPQLPPENVMPTIFVCQESFSDESQRKSLEEPEKRISEENSSLLLVQEELDPDSLSDTSKSDDGVISVRKSAKASNTYRNGWKGEESHSREPSVQRTSVPSNEKKSTCFYVGNDDVGSVKQTSFGLSSKDVIKGPESHIKMKVNLHITAETPSSGKAVNQFPKKDNGSAKDPLSFVRQESFTKETSSSNVLPNKLPHISTHPLLKDLSVTKSNHDYSKETRLILKETETALAALEAKLFTQSHLDEIENTPCLRDDSLSGDSDVDTASTVSLVSDKNVPSHSQKNRIVSLQKEKSSSTSSIQEQYCQPSARERLSEKRRTVPADAGTRNVTKRLGMTRSTGARGSLDFTDEERCSSLPYMPVSETVSSDYEHSSSRHISRRKPFGQTCKDESSRSSNAQKVQQALTRSNSLSTPRPTRASKLRRARLGESSDNESADVERSNISPGTSSANSSSAKSSTETKKPSRLDILAMPRKRAGSFNVPSDSETTSSVRGMFSGRSIDQSYTSRKPAVAESKQPPKKPLPPTQKQTPRPRSSSVKYSSSSTSRRRQQGSDYVSTSEEECGSNHSTPKHKHSRASTATQTSRSSSVSRRQMPNSRQDEEEDEQEVYNNFMAQSVEIAEIARLSQTLVKDVASLAREIHDVAGDGDSQSSSGTGQSTSISSVPNTPASTISAREELVHHIPEASLNYQKIPPGSTGLEDFDQNMNDSREDPSKRRARNIEEVIFDNLMLNPVSHLSHTICANTEVLTEKMKILFQNTEKNWEEIEAKINSENEVPILKTSNKEISSILKELRRVQKQLEVINAIIDRSGHLDVPSSNKKTSSTILTSNPLSRTTNNSAARTESQTPGHVRNYMHKSSSSSSRSPGSSFSRDDEETYIV

Target ORF information:

RefSeq Version	XM_031890593.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis centrosomal protein 170B (cep170b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890593.1

ORF Insert Sequence:

ATGAGTGTCA CATCATGGTT CCTTGTCAGC AGCTCTGGCA CGCGCCATCG GCTGCCACGG 
GAAATGATCT TTGTTGGTCG GGAGGATTGT GAGCTTATGC TTCAGTCCCG CAGTGTGGAT 
AAGCAGCATG CAGTAATCAA CTATGACAGT GACAAAGATG AACATCGCGT CAAGGACCTT 
GGGAGCTTAA ATGGGACCTT TGTAAATGAT GTAAGGATTC CTGACCAGAA GTACATTACT 
CTGAAGCTGA GTGACAACAT ACGTTTTGGC TACGATATTA ATACGTATGT ACTGGAGCAG 
ATCCAGCACC AGGTACCAGA AGAGGCACTG AAGTACTGTG GCCTTCCAGT TACTGCTTTT 
GAACAATTTC CTTCATCTTC TGGCAAACAT GAAAAGTACA CCAGCCACCT TCAGATGTGT 
TTGAAAACCG CTGCAGCAGG AAGAGAAGAC CAGTTTAAAG AGCATGGTGC CCACGTAGAC 
TCTGCCCAAG CCAAACAGGA CAAAGCTGAC AAGAAAGCTA CTAGTGATAT TCCTGCCTAC 
CGTACACCTT TGTACGGTCA ACCATCTTGG TGGGGAGAGG ATGATGATAA CAAGCTTGAT 
AAGGAAGGCA GAAGACAAGA TGAACACTAT TCAGAAAGGC CAAACGATAT GACTCAACAT 
GAGGAAGAAA TAAATGGTAA TATGAGCTAC AGGGATTCTC AGGATCAGTG TGTGTACCCA 
TTCAGAAGAG AACCTAGTTA CTTTGAAATT CCAACTAAAG ACTTTCAACA GCCTGTAAAG 
CCCCCAGAAA CCCAGGTCTA TGAAATCCCT ACTAAGGACA CAGATGCAGT GCCCCCTGTA 
ACACCCCCTG TCATGCAGAG CCATGCCTCT TTTACAATTG AGTTTGATGA TTGCCAGCCT 
GGAAAAATAA AAATTAAAGA TCATGTAACA AAATTTTCTA TGAGACAGAG GAAAACAGCT 
GGAAAGGAAC CTGCTCCAAC AGAAATGGTG TCAGCAGAAA GTAAGGTGGC TGACTGGCTG 
GTTCAAAATG ACCCAAGTTT GATTAGAAGA CCAGCTCCTG GAGAAGATGT CTACAGTACC 
AAGAGTGACC TTCCTATCCA TAACAGAACT CTTAAAGGTA ACAGACATGA AGATGGAACA 
CAGAGTGATT CAGAAGACCC CTTGGTGGCC CAACCTGAAC AGGAAATTGG AACACCTGAT 
CACCCACAGC TCCAGCGGCA GATCAAGCGC GAACCTGAAG AATTGCTGCA CAATCAGCAG 
GCGTTTGTCA TACAGTTTTT TGATGATGAT GATGCCCCTA GGAAAAAACG GTCCCAGTCT 
TTTACACACA ATGCAAACTC CCCTCAGAAT GACACAGACC CAGTCCTTAA AGCAAAAGCT 
GAAAAGCGAA AGGGCACATT GCATGTTGAA AAAGTGTCAA CTAATGGAAT GGGTTCAACT 
GCTCCAGCAT CAAAATCACT GTCAAGCCCT TCATTCCCTC AAAGATCAAA CTCTTTTAGG 
CGAGAAAAGA CAGAGGACCG CATTTCTTCT GCACCTACGA CAGCAAAGTT ACCAGGGAAG 
AACTATGGAA GTGTTGGGAA GAAATCAAAG CTTGCCCAAG AATTTGCTGC TGAATATATG 
CGTGAACAAG TAGAGGTAGT TAAACAGGCA GCAGAAAAAC CCATGACCTT GCCTTTGTCT 
ACATCAATAT TGCAGCAACC AGCCTCACAA GTGCAAATCT CAAGCCAAGC ACAAACAATG 
CAGCTAACAT CTGATGTAAG AACAGGAAAA GTTAAAAATG AAGAAGAGGA CAACCTAAGT 
GATGCAGGAA CATACACAAT AGAAACAGAG ACACAGGATA GAGAAGTTGA ACAAGCCAGG 
AAAATGATAG ACCAGGTCTT TGGAGTATTT GAATCCGATG AGTATTCCAA GATTGCATCC 
ACAGTTTATA GGCCAGTTAT TAAATTGGGA GAAGAGGAAC CTCTTGCCAG TGAACCAAGT 
GTACCACACA AGCCCATAAT GTCATCAACT CCACCAGTAA AACTAAGTAA TGGGCTTCCG 
GCTGAAACTT TGATTGAAAG AACTGGGTCA AGCTCCAAAC AAAGTCAGAA ATGGGTTTCC 
CGTTGGGCAA GCCTCGCTGA CAGTTATCCC GATGCATCAC CAACATCTCC TTTGGACAGT 
CAGAAGCAAG GCTTAATTGC AGATGATGAA GACGTTATTG AGCGTACAGA GCACCAAGGA 
GAAGCAGATC CCACTGTGCC ATCAAGAACA AGACGGCTCC TTCCACAACT TCCTCCTGAA 
AATGTAATGC CAACAATCTT TGTATGCCAA GAGTCTTTTT CAGATGAATC TCAAAGGAAG 
TCTTTGGAAG AACCAGAAAA GAGAATTTCT GAAGAAAACA GCAGTTTATT ATTAGTTCAA 
GAGGAATTAG ATCCAGACAG TCTTAGTGAT ACCAGCAAGT CTGATGATGG TGTCATATCA 
GTCAGGAAGT CTGCCAAGGC TTCCAACACT TATAGAAATG GATGGAAGGG AGAGGAGTCT 
CATAGTAGGG AACCATCTGT TCAGAGAACT TCAGTACCTT CTAATGAAAA GAAATCAACT 
TGTTTTTATG TTGGAAATGA TGACGTTGGA AGTGTAAAAC AAACAAGCTT TGGATTGTCC 
TCTAAAGATG TGATTAAGGG CCCAGAAAGC CACATTAAGA TGAAAGTTAA TTTACACATA 
ACTGCTGAGA CTCCAAGCTC AGGAAAGGCA GTTAATCAGT TTCCCAAGAA AGATAATGGC 
TCTGCAAAAG ATCCTCTTTC ATTTGTAAGG CAGGAAAGCT TTACCAAAGA GACATCCAGT 
AGCAATGTTT TACCAAATAA GCTTCCACAT ATTTCAACTC ATCCATTGCT TAAAGATTTA 
AGTGTTACAA AATCAAATCA TGACTACAGT AAGGAGACAC GCCTAATACT TAAAGAGACA 
GAAACCGCTT TGGCTGCTTT AGAAGCTAAG CTCTTTACAC AGTCCCACCT TGATGAAATA 
GAAAATACTC CTTGTTTGCG TGATGACTCC TTATCAGGAG ACTCTGATGT GGATACAGCC 
AGCACAGTTA GTCTAGTCAG TGATAAAAAT GTACCAAGTC ATTCACAGAA AAACAGAATT 
GTCAGCCTTC AGAAAGAAAA GTCTTCCTCC ACTTCTTCTA TACAAGAGCA ATATTGTCAA 
CCAAGTGCTC GAGAACGTCT TTCTGAGAAA CGAAGGACAG TTCCTGCTGA TGCTGGAACT 
AGAAATGTAA CCAAACGACT AGGGATGACA CGTAGCACTG GAGCACGTGG CTCATTAGAC 
TTCACAGATG AAGAAAGGTG TTCTAGTCTT CCTTATATGC CAGTTTCAGA AACTGTTTCA 
TCTGATTATG AACATTCTTC TTCAAGGCAT ATTTCAAGGA GGAAACCCTT TGGTCAGACT 
TGCAAAGATG AATCGAGTAG ATCATCAAAT GCACAAAAAG TTCAGCAGGC TCTTACACGC 
TCTAACAGTT TATCAACTCC GAGGCCTACA AGAGCTTCCA AACTGCGTCG CGCTCGTCTC 
GGCGAATCTT CTGACAATGA AAGTGCTGAT GTAGAAAGAT CAAATATAAG TCCAGGAACG 
TCTTCTGCAA ACTCATCTTC TGCTAAAAGT TCTACAGAAA CTAAAAAGCC ATCTCGTTTA 
GATATCCTTG CTATGCCAAG AAAACGAGCA GGTTCATTTA ATGTTCCAAG TGACTCTGAA 
ACAACTTCTT CAGTAAGAGG AATGTTTTCA GGACGTAGCA TAGACCAAAG TTACACTAGT 
AGAAAACCAG CTGTGGCAGA AAGCAAGCAG CCTCCCAAGA AACCTCTACC ACCTACACAG 
AAGCAAACAC CAAGACCTCG CTCAAGCAGT GTCAAGTACT CATCATCTTC TACTTCACGA 
AGGAGGCAAC AGGGATCAGA TTATGTTTCT ACATCTGAGG AGGAGTGTGG CTCAAATCAC 
AGCACTCCTA AACACAAACA CTCCCGTGCT TCAACAGCCA CTCAGACTTC AAGATCAAGC 
AGTGTCTCTC GACGTCAGAT GCCCAACAGT CGCCAAGATG AGGAAGAGGA TGAGCAGGAA 
GTTTATAACA ACTTCATGGC CCAGTCTGTG GAAATTGCAG AAATAGCCAG ATTGAGTCAA 
ACCCTGGTGA AAGATGTTGC CAGTTTGGCA AGAGAGATTC ATGATGTAGC AGGAGATGGA 
GATTCTCAGA GTTCCTCAGG AACAGGACAG AGCACCTCAA TCAGTTCTGT GCCAAACACC 
CCAGCCTCAA CCATTTCAGC AAGGGAAGAG TTGGTGCATC ATATACCAGA AGCAAGCCTG 
AACTACCAGA AGATTCCTCC AGGTTCAACA GGCCTTGAAG ACTTTGATCA GAATATGAAT 
GACAGTCGAG AAGATCCATC AAAGAGAAGA GCTAGGAACA TAGAAGAGGT GATTTTTGAC 
AATCTTATGC TGAATCCTGT GTCTCATTTA TCACATACAA TATGCGCAAA CACTGAGGTC 
CTTACAGAAA AAATGAAAAT TTTATTTCAG AATACAGAGA AGAACTGGGA AGAAATTGAA 
GCAAAGATTA ACTCTGAAAA CGAAGTGCCA ATTCTTAAAA CATCCAATAA GGAAATCAGC 
TCTATCTTGA AAGAGTTGAG AAGGGTTCAG AAACAATTGG AAGTTATAAA TGCAATCATC 
GACCGCTCTG GACATCTAGA TGTTCCGTCA AGTAATAAAA AGACATCTTC GACTATCCTG 
ACTTCAAATC CTTTATCTAG GACTACTAAC AATTCAGCTG CCAGAACAGA GTCTCAAACT 
CCTGGCCATG TGAGAAATTA CATGCATAAA TCCAGCTCCA GCTCCTCAAG ATCCCCAGGA 
TCCAGTTTCA GTCGGGATGA CGAAGAAACA TATATTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep170b ( XM_031890594.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746454.1 Xenopus tropicalis centrosomal protein 170B (cep170b), transcript variant X3, mRNA.

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CloneID	OXa63818
Clone ID Related Accession (Same CDS sequence)	XM_031890594.1
Accession Version	XM_031890594.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	centrosomal protein of 170 kDa protein B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTCA CATCATGGTT CCTTGTCAGC AGCTCTGGCA CGCGCCATCG GCTGCCACGG 
GAAATGATCT TTGTTGGTCG GGAGGATTGT GAGCTTATGC TTCAGTCCCG CAGTGTGGAT 
AAGCAGCATG CAGTAATCAA CTATGACAGT GACAAAGATG AACATCGCGT CAAGGACCTT 
GGGAGCTTAA ATGGGACCTT TGTAAATGAT GTAAGGATTC CTGACCAGAA GTACATTACT 
CTGAAGCTGA GTGACAACAT ACGTTTTGGC TACGATATTA ATACGTATGT ACTGGAGCAG 
ATCCAGCACC AGGTACCAGA AGAGGCACTG AAGTACTGTG GCCTTCCAGT TACTGCTTTT 
GAACAATTTC CTTCATCTTC TGGCAAACAT GAAAAGTACA CCAGCCACCT TCAGATGTGT 
TTGAAAACCG CTGCAGCAGG AAGAGAAGAC CAGTTTAAAG AGCATGGTGC CCACGTAGAC 
TCTGCCCAAG CCAAACAGGA CAAAGCTGAC AAGAAAGCTA CTAGTGATAT TCCTGCCTAC 
CGTACACCTT TGTACGGTCA ACCATCTTGG TGGGGAGAGG ATGATGATAA CAAGCTTGAT 
AAGGAAGGCA GAAGACAAGA TGAACACTAT TCAGAAAGGC CAAACGATAT GACTCAACAT 
GAGGAAGAAA TAAATGGTAA TATGAGCTAC AGGGATTCTC AGGATCAGTG TGTGTACCCA 
TTCAGAAGAG AACCTAGTTA CTTTGAAATT CCAACTAAAG ACTTTCAACA GCCTGTAAAG 
CCCCCAGAAA CCCAGGTCTA TGAAATCCCT ACTAAGGACA CAGATGCAGT GCCCCCTGTA 
ACACCCCCTG TCATGCAGAG CCATGCCTCT TTTACAATTG AGTTTGATGA TTGCCAGCCT 
GGAAAAATAA AAATTAAAGA TCATGTAACA AAATTTTCTA TGAGACAGAG GAAAACAGCT 
GGAAAGGAAC CTGCTCCAAC AGAAATGGTG TCAGCAGAAA GTAAGGTGGC TGACTGGCTG 
GTTCAAAATG ACCCAAGTTT GATTAGAAGA CCAGCTCCTG GAGAAGATGT CTACAGTACC 
AAGAGTGACC TTCCTATCCA TAACAGAACT CTTAAAGGTA ACAGACATGA AGATGGAACA 
CAGAGTGATT CAGAAGACCC CTTGGTGGCC CAACCTGAAC AGGAAATTGG AACACCTGAT 
CACCCACAGC TCCAGCGGCA GATCAAGCGC GAACCTGAAG AATTGCTGCA CAATCAGCAG 
GCGTTTGTCA TACAGTTTTT TGATGATGAT GATGCCCCTA GGAAAAAACG GTCCCAGTCT 
TTTACACACA ATGCAAACTC CCCTCAGAAT GACACAGACC CAGTCCTTAA AGCAAAAGCT 
GAAAAGCGAA AGGGCACATT GCATGTTGAA AAAGTGTCAA CTAATGGAAT GGGTTCAACT 
GCTCCAGCAT CAAAATCACT GTCAAGCCCT TCATTCCCTC AAAGATCAAA CTCTTTTAGG 
CGAGAAAAGA CAGAGGACCG CATTTCTTCT GCACCTACGA CAGCAAAGTT ACCAGGGAAG 
AACTATGGAA GTGTTGGGAA GAAATCAAAG CTTGCCCAAG AATTTGCTGC TGAATATATG 
CGTGAACAAG TAGAGGTAGT TAAACAGGCA GCAGAAAAAC CCATGACCTT GCCTTTGTCT 
ACATCAATAT TGCAGCAACC AGCCTCACAA GTGCAAATCT CAAGCCAAGC ACAAACAATG 
CAGCTAACAT CTGATGTAAG AACAGGAAAA GTTAAAAATG AAGAAGAGGA CAACCTAAGT 
GATGCAGGAA CATACACAAT AGAAACAGAG ACACAGGATA GAGAAGTTGA ACAAGCCAGG 
AAAATGATAG ACCAGGTCTT TGGAGTATTT GAATCCGATG AGTATTCCAA GATTGCATCC 
ACAGTTTATA GGCCAGTTAT TAAATTGGGA GAAGAGGAAC CTCTTGCCAG TGAACCAAGT 
GTACCACACA AGCCCATAAT GTCATCAACT CCACCAGTAA AACTAAGTAA TGGGCTTCCG 
GCTGAAACTT TGATTGAAAG AACTGGGTCA AGCTCCAAAC AAAGTCAGAA ATGGGTTTCC 
CGTTGGGCAA GCCTCGCTGA CAGTTATCCC GATGCATCAC CAACATCTCC TTTGGACAGT 
CAGAAGCAAG GCTTAATTGC AGATGATGAA GACGTTATTG AGCGTACAGA GCACCAAGGA 
GAAGCAGATC CCACTGTGCC ATCAAGAACA AGACGGCTCC TTCCACAACT TCCTCCTGAA 
AATGTAATGC CAACAATCTT TGTATGCCAA GAGTCTTTTT CAGATGAATC TCAAAGGAAG 
TCTTTGGAAG AACCAGAAAA GAGAATTTCT GAAGAAAACA GCAGTTTATT ATTAGTTCAA 
GAGGAATTAG ATCCAGACAG TCTTAGTGAT ACCAGCAAGT CTGATGATGG TGTCATATCA 
GTCAGGAAGT CTGCCAAGGC TTCCAACACT TATAGAAATG GATGGAAGGG AGAGGAGTCT 
CATAGTAGGG AACCATCTGT TCAGAGAACT TCAGTACCTT CTAATGAAAA GAAATCAACT 
TGTTTTTATG TTGGAAATGA TGACGTTGGA AGTGTAAAAC AAACAAGCTT TGGATTGTCC 
TCTAAAGATG TGATTAAGGG CCCAGAAAGC CACATTAAGA TGAAAGTTAA TTTACACATA 
ACTGCTGAGA CTCCAAGCTC AGGAAAGGCA GTTAATCAGT TTCCCAAGAA AGATAATGGC 
TCTGCAAAAG ATCCTCTTTC ATTTGTAAGG CAGGAAAGCT TTACCAAAGA GACATCCAGT 
AGCAATGTTT TACCAAATAA GCTTCCACAT ATTTCAACTC ATCCATTGCT TAAAGATTTA 
AGTGTTACAA AATCAAATCA TGACTACAGT AAGGAGACAC GCCTAATACT TAAAGAGACA 
GAAACCGCTT TGGCTGCTTT AGAAGCTAAG CTCTTTACAC AGTCCCACCT TGATGAAATA 
GAAAATACTC CTTGTTTGCG TGATGACTCC TTATCAGGAG ACTCTGATGT GGATACAGCC 
AGCACAGTTA GTCTAGTCAG TGATAAAAAT GTACCAAGTC ATTCACAGAA AAACAGAATT 
GTCAGCCTTC AGAAAGAAAA GTCTTCCTCC ACTTCTTCTA TACAAGAGCA ATATTGTCAA 
CCAAGTGCTC GAGAACGTCT TTCTGAGAAA CGAAGGACAG TTCCTGCTGA TGCTGGAACT 
AGAAATGTAA CCAAACGACT AGGGATGACA CGTAGCACTG GAGCACGTGG CTCATTAGAC 
TTCACAGATG AAGAAAGGTG TTCTAGTCTT CCTTATATGC CAGTTTCAGA AACTGTTTCA 
TCTGATTATG AACATTCTTC TTCAAGGCAT ATTTCAAGGA GGAAACCCTT TGGTCAGACT 
TGCAAAGATG AATCGAGTAG ATCATCAAAT GCACAAAAAG TTCAGCAGGC TCTTACACGC 
TCTAACAGTT TATCAACTCC GAGGCCTACA AGAGCTTCCA AACTGCGTCG CGCTCGTCTC 
GGCGAATCTT CTGACAATGA AAGTGCTGAT GTAGAAAGAT CAAATATAAG TCCAGGAACG 
TCTTCTGCAA ACTCATCTTC TGCTAAAAGT TCTACAGAAA CTAAAAAGCC ATCTCGTTTA 
GATATCCTTG CTATGCCAAG AAAACGAGCA GGTTCATTTA ATGTTCCAAG TGACTCTGAA 
ACAACTTCTT CAGTAAGAGG AATGTTTTCA GGACGTAGCA TAGACCAAAG TTACACTAGT 
AGAAAACCAG CTGTGGCAGA AAGCAAGCAG CCTCCCAAGA AACCTCTACC ACCTACACAG 
AAGCAAACAC CAAGACCTCG CTCAAGCAGT GTCAAGTACT CATCATCTTC TACCACTCAG 
ACTTCAAGAT CAAGCAGTGT CTCTCGACGT CAGATGCCCA ACAGTCGCCA AGATGAGGAA 
GAGGATGAGC AGGAAGTTTA TAACAACTTC ATGGCCCAGT CTGTGGAAAT TGCAGAAATA 
GCCAGATTGA GTCAAACCCT GGTGAAAGAT GTTGCCAGTT TGGCAAGAGA GATTCATGAT 
GTAGCAGGAG ATGGAGATTC TCAGAGTTCC TCAGGAACAG GACAGAGCAC CTCAATCAGT 
TCTGTGCCAA ACACCCCAGC CTCAACCATT TCAGCAAGGG AAGAGATAAT TAAACGGTCC 
TTACAACGAA CGTGCTCATC ACAGTTGGTG CATCATATAC CAGAAGCAAG CCTGAACTAC 
CAGAAGATTC CTCCAGGTTC AACAGGCCTT GAAGACTTTG ATCAGAATAT GAATGACAGT 
CGAGAAGATC CATCAAAGAG AAGAGCTAGG AACATAGAAG AGGTGATTTT TGACAATCTT 
ATGCTGAATC CTGTGTCTCA TTTATCACAT ACAATATGCG CAAACACTGA GGTCCTTACA 
GAAAAAATGA AAATTTTATT TCAGAATACA GAGAAGAACT GGGAAGAAAT TGAAGCAAAG 
ATTAACTCTG AAAACGAAGT GCCAATTCTT AAAACATCCA ATAAGGAAAT CAGCTCTATC 
TTGAAAGAGT TGAGAAGGGT TCAGAAACAA TTGGAAGTTA TAAATGCAAT CATCGACCGC 
TCTGGACATC TAGATGTTCC GTCAAGTAAT AAAAAGACAT CTTCGACTAT CCTGACTTCA 
AATCCTTTAT CTAGGACTAC TAACAATTCA GCTGCCAGAA CAGAGTCTCA AACTCCTGGC 
CATGTGAGAA ATTACATGCA TAAATCCAGC TCCAGCTCCT CAAGATCCCC AGGATCCAGT 
TTCAGTCGGG ATGACGAAGA AACATATATT GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746454.1
CDS	248..5083
Translation	MSVTSWFLVSSSGTRHRLPREMIFVGREDCELMLQSRSVDKQHAVINYDSDKDEHRVKDLGSLNGTFVNDVRIPDQKYITLKLSDNIRFGYDINTYVLEQIQHQVPEEALKYCGLPVTAFEQFPSSSGKHEKYTSHLQMCLKTAAAGREDQFKEHGAHVDSAQAKQDKADKKATSDIPAYRTPLYGQPSWWGEDDDNKLDKEGRRQDEHYSERPNDMTQHEEEINGNMSYRDSQDQCVYPFRREPSYFEIPTKDFQQPVKPPETQVYEIPTKDTDAVPPVTPPVMQSHASFTIEFDDCQPGKIKIKDHVTKFSMRQRKTAGKEPAPTEMVSAESKVADWLVQNDPSLIRRPAPGEDVYSTKSDLPIHNRTLKGNRHEDGTQSDSEDPLVAQPEQEIGTPDHPQLQRQIKREPEELLHNQQAFVIQFFDDDDAPRKKRSQSFTHNANSPQNDTDPVLKAKAEKRKGTLHVEKVSTNGMGSTAPASKSLSSPSFPQRSNSFRREKTEDRISSAPTTAKLPGKNYGSVGKKSKLAQEFAAEYMREQVEVVKQAAEKPMTLPLSTSILQQPASQVQISSQAQTMQLTSDVRTGKVKNEEEDNLSDAGTYTIETETQDREVEQARKMIDQVFGVFESDEYSKIASTVYRPVIKLGEEEPLASEPSVPHKPIMSSTPPVKLSNGLPAETLIERTGSSSKQSQKWVSRWASLADSYPDASPTSPLDSQKQGLIADDEDVIERTEHQGEADPTVPSRTRRLLPQLPPENVMPTIFVCQESFSDESQRKSLEEPEKRISEENSSLLLVQEELDPDSLSDTSKSDDGVISVRKSAKASNTYRNGWKGEESHSREPSVQRTSVPSNEKKSTCFYVGNDDVGSVKQTSFGLSSKDVIKGPESHIKMKVNLHITAETPSSGKAVNQFPKKDNGSAKDPLSFVRQESFTKETSSSNVLPNKLPHISTHPLLKDLSVTKSNHDYSKETRLILKETETALAALEAKLFTQSHLDEIENTPCLRDDSLSGDSDVDTASTVSLVSDKNVPSHSQKNRIVSLQKEKSSSTSSIQEQYCQPSARERLSEKRRTVPADAGTRNVTKRLGMTRSTGARGSLDFTDEERCSSLPYMPVSETVSSDYEHSSSRHISRRKPFGQTCKDESSRSSNAQKVQQALTRSNSLSTPRPTRASKLRRARLGESSDNESADVERSNISPGTSSANSSSAKSSTETKKPSRLDILAMPRKRAGSFNVPSDSETTSSVRGMFSGRSIDQSYTSRKPAVAESKQPPKKPLPPTQKQTPRPRSSSVKYSSSSTTQTSRSSSVSRRQMPNSRQDEEEDEQEVYNNFMAQSVEIAEIARLSQTLVKDVASLAREIHDVAGDGDSQSSSGTGQSTSISSVPNTPASTISAREEIIKRSLQRTCSSQLVHHIPEASLNYQKIPPGSTGLEDFDQNMNDSREDPSKRRARNIEEVIFDNLMLNPVSHLSHTICANTEVLTEKMKILFQNTEKNWEEIEAKINSENEVPILKTSNKEISSILKELRRVQKQLEVINAIIDRSGHLDVPSSNKKTSSTILTSNPLSRTTNNSAARTESQTPGHVRNYMHKSSSSSSRSPGSSFSRDDEETYIV

Target ORF information:

RefSeq Version	XM_031890594.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis centrosomal protein 170B (cep170b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890594.1

ORF Insert Sequence:

ATGAGTGTCA CATCATGGTT CCTTGTCAGC AGCTCTGGCA CGCGCCATCG GCTGCCACGG 
GAAATGATCT TTGTTGGTCG GGAGGATTGT GAGCTTATGC TTCAGTCCCG CAGTGTGGAT 
AAGCAGCATG CAGTAATCAA CTATGACAGT GACAAAGATG AACATCGCGT CAAGGACCTT 
GGGAGCTTAA ATGGGACCTT TGTAAATGAT GTAAGGATTC CTGACCAGAA GTACATTACT 
CTGAAGCTGA GTGACAACAT ACGTTTTGGC TACGATATTA ATACGTATGT ACTGGAGCAG 
ATCCAGCACC AGGTACCAGA AGAGGCACTG AAGTACTGTG GCCTTCCAGT TACTGCTTTT 
GAACAATTTC CTTCATCTTC TGGCAAACAT GAAAAGTACA CCAGCCACCT TCAGATGTGT 
TTGAAAACCG CTGCAGCAGG AAGAGAAGAC CAGTTTAAAG AGCATGGTGC CCACGTAGAC 
TCTGCCCAAG CCAAACAGGA CAAAGCTGAC AAGAAAGCTA CTAGTGATAT TCCTGCCTAC 
CGTACACCTT TGTACGGTCA ACCATCTTGG TGGGGAGAGG ATGATGATAA CAAGCTTGAT 
AAGGAAGGCA GAAGACAAGA TGAACACTAT TCAGAAAGGC CAAACGATAT GACTCAACAT 
GAGGAAGAAA TAAATGGTAA TATGAGCTAC AGGGATTCTC AGGATCAGTG TGTGTACCCA 
TTCAGAAGAG AACCTAGTTA CTTTGAAATT CCAACTAAAG ACTTTCAACA GCCTGTAAAG 
CCCCCAGAAA CCCAGGTCTA TGAAATCCCT ACTAAGGACA CAGATGCAGT GCCCCCTGTA 
ACACCCCCTG TCATGCAGAG CCATGCCTCT TTTACAATTG AGTTTGATGA TTGCCAGCCT 
GGAAAAATAA AAATTAAAGA TCATGTAACA AAATTTTCTA TGAGACAGAG GAAAACAGCT 
GGAAAGGAAC CTGCTCCAAC AGAAATGGTG TCAGCAGAAA GTAAGGTGGC TGACTGGCTG 
GTTCAAAATG ACCCAAGTTT GATTAGAAGA CCAGCTCCTG GAGAAGATGT CTACAGTACC 
AAGAGTGACC TTCCTATCCA TAACAGAACT CTTAAAGGTA ACAGACATGA AGATGGAACA 
CAGAGTGATT CAGAAGACCC CTTGGTGGCC CAACCTGAAC AGGAAATTGG AACACCTGAT 
CACCCACAGC TCCAGCGGCA GATCAAGCGC GAACCTGAAG AATTGCTGCA CAATCAGCAG 
GCGTTTGTCA TACAGTTTTT TGATGATGAT GATGCCCCTA GGAAAAAACG GTCCCAGTCT 
TTTACACACA ATGCAAACTC CCCTCAGAAT GACACAGACC CAGTCCTTAA AGCAAAAGCT 
GAAAAGCGAA AGGGCACATT GCATGTTGAA AAAGTGTCAA CTAATGGAAT GGGTTCAACT 
GCTCCAGCAT CAAAATCACT GTCAAGCCCT TCATTCCCTC AAAGATCAAA CTCTTTTAGG 
CGAGAAAAGA CAGAGGACCG CATTTCTTCT GCACCTACGA CAGCAAAGTT ACCAGGGAAG 
AACTATGGAA GTGTTGGGAA GAAATCAAAG CTTGCCCAAG AATTTGCTGC TGAATATATG 
CGTGAACAAG TAGAGGTAGT TAAACAGGCA GCAGAAAAAC CCATGACCTT GCCTTTGTCT 
ACATCAATAT TGCAGCAACC AGCCTCACAA GTGCAAATCT CAAGCCAAGC ACAAACAATG 
CAGCTAACAT CTGATGTAAG AACAGGAAAA GTTAAAAATG AAGAAGAGGA CAACCTAAGT 
GATGCAGGAA CATACACAAT AGAAACAGAG ACACAGGATA GAGAAGTTGA ACAAGCCAGG 
AAAATGATAG ACCAGGTCTT TGGAGTATTT GAATCCGATG AGTATTCCAA GATTGCATCC 
ACAGTTTATA GGCCAGTTAT TAAATTGGGA GAAGAGGAAC CTCTTGCCAG TGAACCAAGT 
GTACCACACA AGCCCATAAT GTCATCAACT CCACCAGTAA AACTAAGTAA TGGGCTTCCG 
GCTGAAACTT TGATTGAAAG AACTGGGTCA AGCTCCAAAC AAAGTCAGAA ATGGGTTTCC 
CGTTGGGCAA GCCTCGCTGA CAGTTATCCC GATGCATCAC CAACATCTCC TTTGGACAGT 
CAGAAGCAAG GCTTAATTGC AGATGATGAA GACGTTATTG AGCGTACAGA GCACCAAGGA 
GAAGCAGATC CCACTGTGCC ATCAAGAACA AGACGGCTCC TTCCACAACT TCCTCCTGAA 
AATGTAATGC CAACAATCTT TGTATGCCAA GAGTCTTTTT CAGATGAATC TCAAAGGAAG 
TCTTTGGAAG AACCAGAAAA GAGAATTTCT GAAGAAAACA GCAGTTTATT ATTAGTTCAA 
GAGGAATTAG ATCCAGACAG TCTTAGTGAT ACCAGCAAGT CTGATGATGG TGTCATATCA 
GTCAGGAAGT CTGCCAAGGC TTCCAACACT TATAGAAATG GATGGAAGGG AGAGGAGTCT 
CATAGTAGGG AACCATCTGT TCAGAGAACT TCAGTACCTT CTAATGAAAA GAAATCAACT 
TGTTTTTATG TTGGAAATGA TGACGTTGGA AGTGTAAAAC AAACAAGCTT TGGATTGTCC 
TCTAAAGATG TGATTAAGGG CCCAGAAAGC CACATTAAGA TGAAAGTTAA TTTACACATA 
ACTGCTGAGA CTCCAAGCTC AGGAAAGGCA GTTAATCAGT TTCCCAAGAA AGATAATGGC 
TCTGCAAAAG ATCCTCTTTC ATTTGTAAGG CAGGAAAGCT TTACCAAAGA GACATCCAGT 
AGCAATGTTT TACCAAATAA GCTTCCACAT ATTTCAACTC ATCCATTGCT TAAAGATTTA 
AGTGTTACAA AATCAAATCA TGACTACAGT AAGGAGACAC GCCTAATACT TAAAGAGACA 
GAAACCGCTT TGGCTGCTTT AGAAGCTAAG CTCTTTACAC AGTCCCACCT TGATGAAATA 
GAAAATACTC CTTGTTTGCG TGATGACTCC TTATCAGGAG ACTCTGATGT GGATACAGCC 
AGCACAGTTA GTCTAGTCAG TGATAAAAAT GTACCAAGTC ATTCACAGAA AAACAGAATT 
GTCAGCCTTC AGAAAGAAAA GTCTTCCTCC ACTTCTTCTA TACAAGAGCA ATATTGTCAA 
CCAAGTGCTC GAGAACGTCT TTCTGAGAAA CGAAGGACAG TTCCTGCTGA TGCTGGAACT 
AGAAATGTAA CCAAACGACT AGGGATGACA CGTAGCACTG GAGCACGTGG CTCATTAGAC 
TTCACAGATG AAGAAAGGTG TTCTAGTCTT CCTTATATGC CAGTTTCAGA AACTGTTTCA 
TCTGATTATG AACATTCTTC TTCAAGGCAT ATTTCAAGGA GGAAACCCTT TGGTCAGACT 
TGCAAAGATG AATCGAGTAG ATCATCAAAT GCACAAAAAG TTCAGCAGGC TCTTACACGC 
TCTAACAGTT TATCAACTCC GAGGCCTACA AGAGCTTCCA AACTGCGTCG CGCTCGTCTC 
GGCGAATCTT CTGACAATGA AAGTGCTGAT GTAGAAAGAT CAAATATAAG TCCAGGAACG 
TCTTCTGCAA ACTCATCTTC TGCTAAAAGT TCTACAGAAA CTAAAAAGCC ATCTCGTTTA 
GATATCCTTG CTATGCCAAG AAAACGAGCA GGTTCATTTA ATGTTCCAAG TGACTCTGAA 
ACAACTTCTT CAGTAAGAGG AATGTTTTCA GGACGTAGCA TAGACCAAAG TTACACTAGT 
AGAAAACCAG CTGTGGCAGA AAGCAAGCAG CCTCCCAAGA AACCTCTACC ACCTACACAG 
AAGCAAACAC CAAGACCTCG CTCAAGCAGT GTCAAGTACT CATCATCTTC TACCACTCAG 
ACTTCAAGAT CAAGCAGTGT CTCTCGACGT CAGATGCCCA ACAGTCGCCA AGATGAGGAA 
GAGGATGAGC AGGAAGTTTA TAACAACTTC ATGGCCCAGT CTGTGGAAAT TGCAGAAATA 
GCCAGATTGA GTCAAACCCT GGTGAAAGAT GTTGCCAGTT TGGCAAGAGA GATTCATGAT 
GTAGCAGGAG ATGGAGATTC TCAGAGTTCC TCAGGAACAG GACAGAGCAC CTCAATCAGT 
TCTGTGCCAA ACACCCCAGC CTCAACCATT TCAGCAAGGG AAGAGATAAT TAAACGGTCC 
TTACAACGAA CGTGCTCATC ACAGTTGGTG CATCATATAC CAGAAGCAAG CCTGAACTAC 
CAGAAGATTC CTCCAGGTTC AACAGGCCTT GAAGACTTTG ATCAGAATAT GAATGACAGT 
CGAGAAGATC CATCAAAGAG AAGAGCTAGG AACATAGAAG AGGTGATTTT TGACAATCTT 
ATGCTGAATC CTGTGTCTCA TTTATCACAT ACAATATGCG CAAACACTGA GGTCCTTACA 
GAAAAAATGA AAATTTTATT TCAGAATACA GAGAAGAACT GGGAAGAAAT TGAAGCAAAG 
ATTAACTCTG AAAACGAAGT GCCAATTCTT AAAACATCCA ATAAGGAAAT CAGCTCTATC 
TTGAAAGAGT TGAGAAGGGT TCAGAAACAA TTGGAAGTTA TAAATGCAAT CATCGACCGC 
TCTGGACATC TAGATGTTCC GTCAAGTAAT AAAAAGACAT CTTCGACTAT CCTGACTTCA 
AATCCTTTAT CTAGGACTAC TAACAATTCA GCTGCCAGAA CAGAGTCTCA AACTCCTGGC 
CATGTGAGAA ATTACATGCA TAAATCCAGC TCCAGCTCCT CAAGATCCCC AGGATCCAGT 
TTCAGTCGGG ATGACGAAGA AACATATATT GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep170b ( XM_031890595.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746455.1 Xenopus tropicalis centrosomal protein 170B (cep170b), transcript variant X4, mRNA.

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CloneID	OXa63819
Clone ID Related Accession (Same CDS sequence)	XM_031890595.1
Accession Version	XM_031890595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	centrosomal protein of 170 kDa protein B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTCA CATCATGGTT CCTTGTCAGC AGCTCTGGCA CGCGCCATCG GCTGCCACGG 
GAAATGATCT TTGTTGGTCG GGAGGATTGT GAGCTTATGC TTCAGTCCCG CAGTGTGGAT 
AAGCAGCATG CAGTAATCAA CTATGACAGT GACAAAGATG AACATCGCGT CAAGGACCTT 
GGGAGCTTAA ATGGGACCTT TGTAAATGAT GTAAGGATTC CTGACCAGAA GTACATTACT 
CTGAAGCTGA GTGACAACAT ACGTTTTGGC TACGATATTA ATACGTATGT ACTGGAGCAG 
ATCCAGCACC AGGTACCAGA AGAGGCACTG AAGCATGAAA AGTACACCAG CCACCTTCAG 
ATGTGTTTGA AAACCGCTGC AGCAGGAAGA GAAGACCAGT TTAAAGAGCA TGGTGCCCAC 
GTAGACTCTG CCCAAGCCAA ACAGGACAAA GCTGACAAGA AAGCTACTAG TGATATTCCT 
GCCTACCGTA CACCTTTGTA CGGTCAACCA TCTTGGTGGG GAGAGGATGA TGATAACAAG 
CTTGATAAGG AAGGCAGAAG ACAAGATGAA CACTATTCAG AAAGGCCAAA CGATATGACT 
CAACATGAGG AAGAAATAAA TGGTAATATG AGCTACAGGG ATTCTCAGGA TCAGTGTGTG 
TACCCATTCA GAAGAGAACC TAGTTACTTT GAAATTCCAA CTAAAGACTT TCAACAGCCT 
GTAAAGCCCC CAGAAACCCA GGTCTATGAA ATCCCTACTA AGGACACAGA TGCAGTGCCC 
CCTGTAACAC CCCCTGTCAT GCAGAGCCAT GCCTCTTTTA CAATTGAGTT TGATGATTGC 
CAGCCTGGAA AAATAAAAAT TAAAGATCAT GTAACAAAAT TTTCTATGAG ACAGAGGAAA 
ACAGCTGGAA AGGAACCTGC TCCAACAGAA ATGGTGTCAG CAGAAAGTAA GGTGGCTGAC 
TGGCTGGTTC AAAATGACCC AAGTTTGATT AGAAGACCAG CTCCTGGAGA AGATGTCTAC 
AGTACCAAGA GTGACCTTCC TATCCATAAC AGAACTCTTA AAGGTAACAG ACATGAAGAT 
GGAACACAGA GTGATTCAGA AGACCCCTTG GTGGCCCAAC CTGAACAGGA AATTGGAACA 
CCTGATCACC CACAGCTCCA GCGGCAGATC AAGCGCGAAC CTGAAGAATT GCTGCACAAT 
CAGCAGGCGT TTGTCATACA GTTTTTTGAT GATGATGATG CCCCTAGGAA AAAACGGTCC 
CAGTCTTTTA CACACAATGC AAACTCCCCT CAGAATGACA CAGACCCAGT CCTTAAAGCA 
AAAGCTGAAA AGCGAAAGGG CACATTGCAT GTTGAAAAAG TGTCAACTAA TGGAATGGGT 
TCAACTGCTC CAGCATCAAA ATCACTGTCA AGCCCTTCAT TCCCTCAAAG ATCAAACTCT 
TTTAGGCGAG AAAAGACAGA GGACCGCATT TCTTCTGCAC CTACGACAGC AAAGTTACCA 
GGGAAGAACT ATGGAAGTGT TGGGAAGAAA TCAAAGCTTG CCCAAGAATT TGCTGCTGAA 
TATATGCGTG AACAAGTAGA GGTAGTTAAA CAGGCAGCAG AAAAACCCAT GACCTTGCCT 
TTGTCTACAT CAATATTGCA GCAACCAGCC TCACAAGTGC AAATCTCAAG CCAAGCACAA 
ACAATGCAGC TAACATCTGA TGTAAGAACA GGAAAAGTTA AAAATGAAGA AGAGGACAAC 
CTAAGTGATG CAGGAACATA CACAATAGAA ACAGAGACAC AGGATAGAGA AGTTGAACAA 
GCCAGGAAAA TGATAGACCA GGTCTTTGGA GTATTTGAAT CCGATGAGTA TTCCAAGATT 
GCATCCACAG TTTATAGGCC AGTTATTAAA TTGGGAGAAG AGGAACCTCT TGCCAGTGAA 
CCAAGTGTAC CACACAAGCC CATAATGTCA TCAACTCCAC CAGTAAAACT AAGTAATGGG 
CTTCCGGCTG AAACTTTGAT TGAAAGAACT GGGTCAAGCT CCAAACAAAG TCAGAAATGG 
GTTTCCCGTT GGGCAAGCCT CGCTGACAGT TATCCCGATG CATCACCAAC ATCTCCTTTG 
GACAGTCAGA AGCAAGGCTT AATTGCAGAT GATGAAGACG TTATTGAGCG TACAGAGCAC 
CAAGGAGAAG CAGATCCCAC TGTGCCATCA AGAACAAGAC GGCTCCTTCC ACAACTTCCT 
CCTGAAAATG TAATGCCAAC AATCTTTGTA TGCCAAGAGT CTTTTTCAGA TGAATCTCAA 
AGGAAGTCTT TGGAAGAACC AGAAAAGAGA ATTTCTGAAG AAAACAGCAG TTTATTATTA 
GTTCAAGAGG AATTAGATCC AGACAGTCTT AGTGATACCA GCAAGTCTGA TGATGGTGTC 
ATATCAGTCA GGAAGTCTGC CAAGGCTTCC AACACTTATA GAAATGGATG GAAGGGAGAG 
GAGTCTCATA GTAGGGAACC ATCTGTTCAG AGAACTTCAG TACCTTCTAA TGAAAAGAAA 
TCAACTTGTT TTTATGTTGG AAATGATGAC GTTGGAAGTG TAAAACAAAC AAGCTTTGGA 
TTGTCCTCTA AAGATGTGAT TAAGGGCCCA GAAAGCCACA TTAAGATGAA AGTTAATTTA 
CACATAACTG CTGAGACTCC AAGCTCAGGA AAGGCAGTTA ATCAGTTTCC CAAGAAAGAT 
AATGGCTCTG CAAAAGATCC TCTTTCATTT GTAAGGCAGG AAAGCTTTAC CAAAGAGACA 
TCCAGTAGCA ATGTTTTACC AAATAAGCTT CCACATATTT CAACTCATCC ATTGCTTAAA 
GATTTAAGTG TTACAAAATC AAATCATGAC TACAGTAAGG AGACACGCCT AATACTTAAA 
GAGACAGAAA CCGCTTTGGC TGCTTTAGAA GCTAAGCTCT TTACACAGTC CCACCTTGAT 
GAAATAGAAA ATACTCCTTG TTTGCGTGAT GACTCCTTAT CAGGAGACTC TGATGTGGAT 
ACAGCCAGCA CAGTTAGTCT AGTCAGTGAT AAAAATGTAC CAAGTCATTC ACAGAAAAAC 
AGAATTGTCA GCCTTCAGAA AGAAAAGTCT TCCTCCACTT CTTCTATACA AGAGCAATAT 
TGTCAACCAA GTGCTCGAGA ACGTCTTTCT GAGAAACGAA GGACAGTTCC TGCTGATGCT 
GGAACTAGAA ATGTAACCAA ACGACTAGGG ATGACACGTA GCACTGGAGC ACGTGGCTCA 
TTAGACTTCA CAGATGAAGA AAGGTGTTCT AGTCTTCCTT ATATGCCAGT TTCAGAAACT 
GTTTCATCTG ATTATGAACA TTCTTCTTCA AGGCATATTT CAAGGAGGAA ACCCTTTGGT 
CAGACTTGCA AAGATGAATC GAGTAGATCA TCAAATGCAC AAAAAGTTCA GCAGGCTCTT 
ACACGCTCTA ACAGTTTATC AACTCCGAGG CCTACAAGAG CTTCCAAACT GCGTCGCGCT 
CGTCTCGGCG AATCTTCTGA CAATGAAAGT GCTGATGTAG AAAGATCAAA TATAAGTCCA 
GGAACGTCTT CTGCAAACTC ATCTTCTGCT AAAAGTTCTA CAGAAACTAA AAAGCCATCT 
CGTTTAGATA TCCTTGCTAT GCCAAGAAAA CGAGCAGGTT CATTTAATGT TCCAAGTGAC 
TCTGAAACAA CTTCTTCAGT AAGAGGAATG TTTTCAGGAC GTAGCATAGA CCAAAGTTAC 
ACTAGTAGAA AACCAGCTGT GGCAGAAAGC AAGCAGCCTC CCAAGAAACC TCTACCACCT 
ACACAGAAGC AAACACCAAG ACCTCGCTCA AGCAGTGTCA AGTACTCATC ATCTTCTACT 
TCACGAAGGA GGCAACAGGG ATCAGATTAT GTTTCTACAT CTGAGGAGGA GTGTGGCTCA 
AATCACAGCA CTCCTAAACA CAAACACTCC CGTGCTTCAA CAGCCACTCA GACTTCAAGA 
TCAAGCAGTG TCTCTCGACG TCAGATGCCC AACAGTCGCC AAGATGAGGA AGAGGATGAG 
CAGGAAGTTT ATAACAACTT CATGGCCCAG TCTGTGGAAA TTGCAGAAAT AGCCAGATTG 
AGTCAAACCC TGGTGAAAGA TGTTGCCAGT TTGGCAAGAG AGATTCATGA TGTAGCAGGA 
GATGGAGATT CTCAGAGTTC CTCAGGAACA GGACAGAGCA CCTCAATCAG TTCTGTGCCA 
AACACCCCAG CCTCAACCAT TTCAGCAAGG GAAGAGTTGG TGCATCATAT ACCAGAAGCA 
AGCCTGAACT ACCAGAAGAT TCCTCCAGGT TCAACAGGCC TTGAAGACTT TGATCAGAAT 
ATGAATGACA GTCGAGAAGA TCCATCAAAG AGAAGAGCTA GGAACATAGA AGAGGTGATT 
TTTGACAATC TTATGCTGAA TCCTGTGTCT CATTTATCAC ATACAATATG CGCAAACACT 
GAGGTCCTTA CAGAAAAAAT GAAAATTTTA TTTCAGAATA CAGAGAAGAA CTGGGAAGAA 
ATTGAAGCAA AGATTAACTC TGAAAACGAA GTGCCAATTC TTAAAACATC CAATAAGGAA 
ATCAGCTCTA TCTTGAAAGA GTTGAGAAGG GTTCAGAAAC AATTGGAAGT TATAAATGCA 
ATCATCGACC GCTCTGGACA TCTAGATGTT CCGTCAAGTA ATAAAAAGAC ATCTTCGACT 
ATCCTGACTT CAAATCCTTT ATCTAGGACT ACTAACAATT CAGCTGCCAG AACAGAGTCT 
CAAACTCCTG GCCATGTGAG AAATTACATG CATAAATCCA GCTCCAGCTC CTCAAGATCC 
CCAGGATCCA GTTTCAGTCG GGATGACGAA GAAACATATA TTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746455.1
CDS	248..5095
Translation	MSVTSWFLVSSSGTRHRLPREMIFVGREDCELMLQSRSVDKQHAVINYDSDKDEHRVKDLGSLNGTFVNDVRIPDQKYITLKLSDNIRFGYDINTYVLEQIQHQVPEEALKHEKYTSHLQMCLKTAAAGREDQFKEHGAHVDSAQAKQDKADKKATSDIPAYRTPLYGQPSWWGEDDDNKLDKEGRRQDEHYSERPNDMTQHEEEINGNMSYRDSQDQCVYPFRREPSYFEIPTKDFQQPVKPPETQVYEIPTKDTDAVPPVTPPVMQSHASFTIEFDDCQPGKIKIKDHVTKFSMRQRKTAGKEPAPTEMVSAESKVADWLVQNDPSLIRRPAPGEDVYSTKSDLPIHNRTLKGNRHEDGTQSDSEDPLVAQPEQEIGTPDHPQLQRQIKREPEELLHNQQAFVIQFFDDDDAPRKKRSQSFTHNANSPQNDTDPVLKAKAEKRKGTLHVEKVSTNGMGSTAPASKSLSSPSFPQRSNSFRREKTEDRISSAPTTAKLPGKNYGSVGKKSKLAQEFAAEYMREQVEVVKQAAEKPMTLPLSTSILQQPASQVQISSQAQTMQLTSDVRTGKVKNEEEDNLSDAGTYTIETETQDREVEQARKMIDQVFGVFESDEYSKIASTVYRPVIKLGEEEPLASEPSVPHKPIMSSTPPVKLSNGLPAETLIERTGSSSKQSQKWVSRWASLADSYPDASPTSPLDSQKQGLIADDEDVIERTEHQGEADPTVPSRTRRLLPQLPPENVMPTIFVCQESFSDESQRKSLEEPEKRISEENSSLLLVQEELDPDSLSDTSKSDDGVISVRKSAKASNTYRNGWKGEESHSREPSVQRTSVPSNEKKSTCFYVGNDDVGSVKQTSFGLSSKDVIKGPESHIKMKVNLHITAETPSSGKAVNQFPKKDNGSAKDPLSFVRQESFTKETSSSNVLPNKLPHISTHPLLKDLSVTKSNHDYSKETRLILKETETALAALEAKLFTQSHLDEIENTPCLRDDSLSGDSDVDTASTVSLVSDKNVPSHSQKNRIVSLQKEKSSSTSSIQEQYCQPSARERLSEKRRTVPADAGTRNVTKRLGMTRSTGARGSLDFTDEERCSSLPYMPVSETVSSDYEHSSSRHISRRKPFGQTCKDESSRSSNAQKVQQALTRSNSLSTPRPTRASKLRRARLGESSDNESADVERSNISPGTSSANSSSAKSSTETKKPSRLDILAMPRKRAGSFNVPSDSETTSSVRGMFSGRSIDQSYTSRKPAVAESKQPPKKPLPPTQKQTPRPRSSSVKYSSSSTSRRRQQGSDYVSTSEEECGSNHSTPKHKHSRASTATQTSRSSSVSRRQMPNSRQDEEEDEQEVYNNFMAQSVEIAEIARLSQTLVKDVASLAREIHDVAGDGDSQSSSGTGQSTSISSVPNTPASTISAREELVHHIPEASLNYQKIPPGSTGLEDFDQNMNDSREDPSKRRARNIEEVIFDNLMLNPVSHLSHTICANTEVLTEKMKILFQNTEKNWEEIEAKINSENEVPILKTSNKEISSILKELRRVQKQLEVINAIIDRSGHLDVPSSNKKTSSTILTSNPLSRTTNNSAARTESQTPGHVRNYMHKSSSSSSRSPGSSFSRDDEETYIV

Target ORF information:

RefSeq Version	XM_031890595.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis centrosomal protein 170B (cep170b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890595.1

ORF Insert Sequence:

ATGAGTGTCA CATCATGGTT CCTTGTCAGC AGCTCTGGCA CGCGCCATCG GCTGCCACGG 
GAAATGATCT TTGTTGGTCG GGAGGATTGT GAGCTTATGC TTCAGTCCCG CAGTGTGGAT 
AAGCAGCATG CAGTAATCAA CTATGACAGT GACAAAGATG AACATCGCGT CAAGGACCTT 
GGGAGCTTAA ATGGGACCTT TGTAAATGAT GTAAGGATTC CTGACCAGAA GTACATTACT 
CTGAAGCTGA GTGACAACAT ACGTTTTGGC TACGATATTA ATACGTATGT ACTGGAGCAG 
ATCCAGCACC AGGTACCAGA AGAGGCACTG AAGCATGAAA AGTACACCAG CCACCTTCAG 
ATGTGTTTGA AAACCGCTGC AGCAGGAAGA GAAGACCAGT TTAAAGAGCA TGGTGCCCAC 
GTAGACTCTG CCCAAGCCAA ACAGGACAAA GCTGACAAGA AAGCTACTAG TGATATTCCT 
GCCTACCGTA CACCTTTGTA CGGTCAACCA TCTTGGTGGG GAGAGGATGA TGATAACAAG 
CTTGATAAGG AAGGCAGAAG ACAAGATGAA CACTATTCAG AAAGGCCAAA CGATATGACT 
CAACATGAGG AAGAAATAAA TGGTAATATG AGCTACAGGG ATTCTCAGGA TCAGTGTGTG 
TACCCATTCA GAAGAGAACC TAGTTACTTT GAAATTCCAA CTAAAGACTT TCAACAGCCT 
GTAAAGCCCC CAGAAACCCA GGTCTATGAA ATCCCTACTA AGGACACAGA TGCAGTGCCC 
CCTGTAACAC CCCCTGTCAT GCAGAGCCAT GCCTCTTTTA CAATTGAGTT TGATGATTGC 
CAGCCTGGAA AAATAAAAAT TAAAGATCAT GTAACAAAAT TTTCTATGAG ACAGAGGAAA 
ACAGCTGGAA AGGAACCTGC TCCAACAGAA ATGGTGTCAG CAGAAAGTAA GGTGGCTGAC 
TGGCTGGTTC AAAATGACCC AAGTTTGATT AGAAGACCAG CTCCTGGAGA AGATGTCTAC 
AGTACCAAGA GTGACCTTCC TATCCATAAC AGAACTCTTA AAGGTAACAG ACATGAAGAT 
GGAACACAGA GTGATTCAGA AGACCCCTTG GTGGCCCAAC CTGAACAGGA AATTGGAACA 
CCTGATCACC CACAGCTCCA GCGGCAGATC AAGCGCGAAC CTGAAGAATT GCTGCACAAT 
CAGCAGGCGT TTGTCATACA GTTTTTTGAT GATGATGATG CCCCTAGGAA AAAACGGTCC 
CAGTCTTTTA CACACAATGC AAACTCCCCT CAGAATGACA CAGACCCAGT CCTTAAAGCA 
AAAGCTGAAA AGCGAAAGGG CACATTGCAT GTTGAAAAAG TGTCAACTAA TGGAATGGGT 
TCAACTGCTC CAGCATCAAA ATCACTGTCA AGCCCTTCAT TCCCTCAAAG ATCAAACTCT 
TTTAGGCGAG AAAAGACAGA GGACCGCATT TCTTCTGCAC CTACGACAGC AAAGTTACCA 
GGGAAGAACT ATGGAAGTGT TGGGAAGAAA TCAAAGCTTG CCCAAGAATT TGCTGCTGAA 
TATATGCGTG AACAAGTAGA GGTAGTTAAA CAGGCAGCAG AAAAACCCAT GACCTTGCCT 
TTGTCTACAT CAATATTGCA GCAACCAGCC TCACAAGTGC AAATCTCAAG CCAAGCACAA 
ACAATGCAGC TAACATCTGA TGTAAGAACA GGAAAAGTTA AAAATGAAGA AGAGGACAAC 
CTAAGTGATG CAGGAACATA CACAATAGAA ACAGAGACAC AGGATAGAGA AGTTGAACAA 
GCCAGGAAAA TGATAGACCA GGTCTTTGGA GTATTTGAAT CCGATGAGTA TTCCAAGATT 
GCATCCACAG TTTATAGGCC AGTTATTAAA TTGGGAGAAG AGGAACCTCT TGCCAGTGAA 
CCAAGTGTAC CACACAAGCC CATAATGTCA TCAACTCCAC CAGTAAAACT AAGTAATGGG 
CTTCCGGCTG AAACTTTGAT TGAAAGAACT GGGTCAAGCT CCAAACAAAG TCAGAAATGG 
GTTTCCCGTT GGGCAAGCCT CGCTGACAGT TATCCCGATG CATCACCAAC ATCTCCTTTG 
GACAGTCAGA AGCAAGGCTT AATTGCAGAT GATGAAGACG TTATTGAGCG TACAGAGCAC 
CAAGGAGAAG CAGATCCCAC TGTGCCATCA AGAACAAGAC GGCTCCTTCC ACAACTTCCT 
CCTGAAAATG TAATGCCAAC AATCTTTGTA TGCCAAGAGT CTTTTTCAGA TGAATCTCAA 
AGGAAGTCTT TGGAAGAACC AGAAAAGAGA ATTTCTGAAG AAAACAGCAG TTTATTATTA 
GTTCAAGAGG AATTAGATCC AGACAGTCTT AGTGATACCA GCAAGTCTGA TGATGGTGTC 
ATATCAGTCA GGAAGTCTGC CAAGGCTTCC AACACTTATA GAAATGGATG GAAGGGAGAG 
GAGTCTCATA GTAGGGAACC ATCTGTTCAG AGAACTTCAG TACCTTCTAA TGAAAAGAAA 
TCAACTTGTT TTTATGTTGG AAATGATGAC GTTGGAAGTG TAAAACAAAC AAGCTTTGGA 
TTGTCCTCTA AAGATGTGAT TAAGGGCCCA GAAAGCCACA TTAAGATGAA AGTTAATTTA 
CACATAACTG CTGAGACTCC AAGCTCAGGA AAGGCAGTTA ATCAGTTTCC CAAGAAAGAT 
AATGGCTCTG CAAAAGATCC TCTTTCATTT GTAAGGCAGG AAAGCTTTAC CAAAGAGACA 
TCCAGTAGCA ATGTTTTACC AAATAAGCTT CCACATATTT CAACTCATCC ATTGCTTAAA 
GATTTAAGTG TTACAAAATC AAATCATGAC TACAGTAAGG AGACACGCCT AATACTTAAA 
GAGACAGAAA CCGCTTTGGC TGCTTTAGAA GCTAAGCTCT TTACACAGTC CCACCTTGAT 
GAAATAGAAA ATACTCCTTG TTTGCGTGAT GACTCCTTAT CAGGAGACTC TGATGTGGAT 
ACAGCCAGCA CAGTTAGTCT AGTCAGTGAT AAAAATGTAC CAAGTCATTC ACAGAAAAAC 
AGAATTGTCA GCCTTCAGAA AGAAAAGTCT TCCTCCACTT CTTCTATACA AGAGCAATAT 
TGTCAACCAA GTGCTCGAGA ACGTCTTTCT GAGAAACGAA GGACAGTTCC TGCTGATGCT 
GGAACTAGAA ATGTAACCAA ACGACTAGGG ATGACACGTA GCACTGGAGC ACGTGGCTCA 
TTAGACTTCA CAGATGAAGA AAGGTGTTCT AGTCTTCCTT ATATGCCAGT TTCAGAAACT 
GTTTCATCTG ATTATGAACA TTCTTCTTCA AGGCATATTT CAAGGAGGAA ACCCTTTGGT 
CAGACTTGCA AAGATGAATC GAGTAGATCA TCAAATGCAC AAAAAGTTCA GCAGGCTCTT 
ACACGCTCTA ACAGTTTATC AACTCCGAGG CCTACAAGAG CTTCCAAACT GCGTCGCGCT 
CGTCTCGGCG AATCTTCTGA CAATGAAAGT GCTGATGTAG AAAGATCAAA TATAAGTCCA 
GGAACGTCTT CTGCAAACTC ATCTTCTGCT AAAAGTTCTA CAGAAACTAA AAAGCCATCT 
CGTTTAGATA TCCTTGCTAT GCCAAGAAAA CGAGCAGGTT CATTTAATGT TCCAAGTGAC 
TCTGAAACAA CTTCTTCAGT AAGAGGAATG TTTTCAGGAC GTAGCATAGA CCAAAGTTAC 
ACTAGTAGAA AACCAGCTGT GGCAGAAAGC AAGCAGCCTC CCAAGAAACC TCTACCACCT 
ACACAGAAGC AAACACCAAG ACCTCGCTCA AGCAGTGTCA AGTACTCATC ATCTTCTACT 
TCACGAAGGA GGCAACAGGG ATCAGATTAT GTTTCTACAT CTGAGGAGGA GTGTGGCTCA 
AATCACAGCA CTCCTAAACA CAAACACTCC CGTGCTTCAA CAGCCACTCA GACTTCAAGA 
TCAAGCAGTG TCTCTCGACG TCAGATGCCC AACAGTCGCC AAGATGAGGA AGAGGATGAG 
CAGGAAGTTT ATAACAACTT CATGGCCCAG TCTGTGGAAA TTGCAGAAAT AGCCAGATTG 
AGTCAAACCC TGGTGAAAGA TGTTGCCAGT TTGGCAAGAG AGATTCATGA TGTAGCAGGA 
GATGGAGATT CTCAGAGTTC CTCAGGAACA GGACAGAGCA CCTCAATCAG TTCTGTGCCA 
AACACCCCAG CCTCAACCAT TTCAGCAAGG GAAGAGTTGG TGCATCATAT ACCAGAAGCA 
AGCCTGAACT ACCAGAAGAT TCCTCCAGGT TCAACAGGCC TTGAAGACTT TGATCAGAAT 
ATGAATGACA GTCGAGAAGA TCCATCAAAG AGAAGAGCTA GGAACATAGA AGAGGTGATT 
TTTGACAATC TTATGCTGAA TCCTGTGTCT CATTTATCAC ATACAATATG CGCAAACACT 
GAGGTCCTTA CAGAAAAAAT GAAAATTTTA TTTCAGAATA CAGAGAAGAA CTGGGAAGAA 
ATTGAAGCAA AGATTAACTC TGAAAACGAA GTGCCAATTC TTAAAACATC CAATAAGGAA 
ATCAGCTCTA TCTTGAAAGA GTTGAGAAGG GTTCAGAAAC AATTGGAAGT TATAAATGCA 
ATCATCGACC GCTCTGGACA TCTAGATGTT CCGTCAAGTA ATAAAAAGAC ATCTTCGACT 
ATCCTGACTT CAAATCCTTT ATCTAGGACT ACTAACAATT CAGCTGCCAG AACAGAGTCT 
CAAACTCCTG GCCATGTGAG AAATTACATG CATAAATCCA GCTCCAGCTC CTCAAGATCC 
CCAGGATCCA GTTTCAGTCG GGATGACGAA GAAACATATA TTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep170b ( NM_001079255.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001072723.1 Xenopus tropicalis centrosomal protein 170B (cep170b), mRNA.

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CloneID	OXa22254
Clone ID Related Accession (Same CDS sequence)	NM_001079255.1
Accession Version	NM_001079255.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Xenopus tropicalis(tropical clawed frog)
Product	centrosomal protein of 170 kDa protein B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC125690.1. ##Evidence-Data-START## Transcript exon combination :: BC125690.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGAGTGTCA CATCATGGTT CCTTGTCAGC AGCTCTGGCA CGCGCCATCG GCTGCCACGG 
GAAATGATCT TTGTTGGTCG GGAGGATTGT GAGCTTATGC TTCAGTCCCG CAGTGTGGAT 
AAGCAGCATG CAGTAATCAA CTATGACAGT GACAAAGATG AACATCGCGT CAAGGACCTT 
GGGAGCTTAA ATGGGACCTT TGTAAATGAT GTAAGGATTC CTGACCAGAA GTACATTACT 
CTGAAGCTGA GTGACAACAT ACGTTTTGGC TACGATATTA ATACGTATGT ACTGGAGCAG 
ATCCAGCACC AGGTACCAGA AGAGGCACTG AAGCATGAAA AGTACACCAG CCACCTTCAG 
ATGTGTTTGA AAACCGCTGC AGCAGGAAGA GAAGACCAGT TTAAAGAGCA TGGTGCCCAC 
GTAGACTCTG CCCAAGCCAA ACAGGACAAA GCTGACAAGA AAGCTACTAG TGATATTCCT 
GCCTACCGTA CACCTTTGTA CGGTCAACCA TCTTGGTGGG GAGAGGATGA TGATAACAAG 
CTTGATAAGG AAGGCAGAAG ACAAGATGAA CACTATTCAG AAAGGCCAAA CGATATGACT 
CAACATGAGG AAGAAATAAA TGGTAATATG AGCTACAGGG ATTCTCAGGA TCAGTGTGTG 
TACCCATTCA GAAGAGAACC TAGTTACTTT GAAATTCCAA CTAAAGACTT TCAACAGCCT 
GTAAAGCCCC CAGAAACCCA GGTCTATGAA ATCCCTACTA AGGACACAGA TGCAGTGCCC 
CCTGTAACAC CCCCTGTCAT GCAGAGCCAT GCCTCTTTTA CAATTGAGTT TGATGATTGC 
CAGCCTGGAA AAATAAAAAT TAAAGATCAT GTAACAAAAT TTTCTATGAG ACAGAGGAAA 
ACAGCTGGAA AGGAACCTGC TCCAACAGAA ATGGTGTCAG CAGAAAGTAA GGTGGCTGAC 
TGGCTGGTTC AAAATGACCC AAGTTTGATT AGAAGACCAG CTCCTGGAGA AGATGTCTAC 
AGTACCAAGA GTGACCTTCC TATCCATAAC AGAACTCTTA AAGGTAACAG ACATGAAGAT 
GGAACACAGA GTGATTCAGA AGACCCCTTG GTGGCCCAAC CTGAACAGGA AATTGGAACA 
CCTGATCACC CACAGCTCCA GCGGCAGATC AAGCGCGAAC CTGAAGAATT GCTGCACAAT 
CAGCAGGCGT TTGTCATACA GTTTTTTGAT GATGATGATG CCCCTAGGAA AAAACGGTCC 
CAGTCTTTTA CACACAATGC AAACTCCCCT CAGAATGACA CAGACCCAGT CCTTAAAGCA 
AAAGCTGAAA AGCGAAAGGG CACATTGCAT GTTGAAAAAG TGTCAACTAA TGGAATGGGT 
TCAACTGCTC CAGCATCAAA ATCACTGTCA AGCCCTTCAT TCCCTCAAAG ATCAAACTCT 
TTTAGGCGAG AAAAGACAGA GGACCGCATT TCTTCTGCAC CTACGACAGC AAAGTTACCA 
GGGAAGAACT ATGGAAGTGT TGGGAAGAAA TCAAAGCTTG CCCAAGAATT TGCTGCTGAA 
TATATGCGTG AACAAGTAGA GGTAGTTAAA CAGGCAGCAG AAAAACCCAT GACCTTGCCT 
TTGTCTACAT CAATATTGCA GCAACCAGCC TCACAAGTGC AAATCTCAAG CCAAGCACAA 
ACAATGCAGC TAACATCTGA TGTAAGAACA GGAAAAGTTA AAAATGAAGA AGAGGACAAC 
CTAAGTGATG CAGGAACATA CACAATAGAA ACAGAGACAC AGGATAGAGA AGTTGAACAA 
GCCAGGAAAA TGATAGACCA GGTCTTTGGA GTATTTGAAT CCGATGAGTA TTCCAAGATT 
GCATCCACAG TTTATAGGCC AGTTATTAAA TTGGGAGAAG AGGAACCTCT TGCCAGTGAA 
CCAAGTGTAC CACACAAGCC CATAATGTCA TCAACTCCAC CAGTAAAACT AAGTAATGGG 
CTTCCGGCTG AAACTTTGAT TGAAAGAACT GGGTCAAGCT CCAAACAAAG TCAGAAATGG 
GTTTCCCGTT GGGCAAGCCT CGCTGACAGT TATCCCGATG CATCACCAAC ATCTCCTTTG 
GACAGTCAGA AGCAAGGCTT AATTGCAGAT GATGAAGACG TTATTGAGCG TACAGAGCAC 
CAAGGAGAAG CAGATCCCAC TGTGCCATCA AGAACAAGAC GGCTCCTTCC ACAACTTCCT 
CCTGAAAATG TAATGCCAAC AATCTTTGTA TGCCAAGAGT CTTTTTCAGA TGAATCTCAA 
AGGAAGTCTT TGGAAGAACC AGAAAAGAGA ATTTCTGAAG AAAACAGCAG TTTATTATTA 
GTTCAAGAGG AATTAGATCC AGACAGTCTT AGTGATACCA GCAAGTCTGA TGATGGTGTC 
ATATCAGTCA GGAAGTCTGC CAAGGCTTCC AACACTTATA GAAATGGATG GAAGGGAGAG 
GAGTCTCATA GTAGGGAACC ATCTGTTCAG AGAACTTCAG TACCTTCTAA TGAAAAGAAA 
TCAACTTGTT TTTATGTTGG AAATGATGAC GTTGGAAGTG TAAAACAAAC AAGCTTTGGA 
TTGTCCTCTA AAGATGTGAT TAAGGGCCCA GAAAGCCACA TTAAGATGAA AGTTAATTTA 
CACATAACTG CTGAGACTCC AAGCTCAGGA AAGGCAGTTA ATCAGTTTCC CAAGAAAGAT 
AATGGCTCTG CAAAAGATCC TCTTTCATTT GTAAGGCAGG AAAGCTTTAC CAAAGAGACA 
TCCAGTAGCA ATGTTTTACC AAATAAGCTT CCACATATTT CAACTCATCC ATTGCTTAAA 
GATTTAAGTG TTACAAAATC AAATCATGAC TACAGTAAGG AGACACGCCT AATACTTAAA 
GAGACAGAAA CCGCTTTGGC TGCTTTAGAA GCTAAGCTCT TTACACAGTC CCACCTTGAT 
GAAATAGAAA ATACTCCTTG TTTGCGTGAT GACTCCTTAT CAGGAGACTC TGATGTGGAT 
ACAGCCAGCA CAGTTAGTCT AGTCAGTGAT AAAAATGTAC CAAGTCATTC ACAGAAAAAC 
AGAATTGTCA GCCTTCAGAA AGAAAAGTCT TCCTCCACTT CTTCTATACA AGAGCAATAT 
TGTCAACCAA GTGCTCGAGA ACGTCTTTCT GAGAAACGAA GGACAGTTCC TGCTGATGCT 
GGAACTAGAA ATGTAACCAA ACGACTAGGG ATGACACGTA GCACTGGAGC ACGTGGCTCA 
TTAGACTTCA CAGATGAAGA AAGGTGTTCT AGTCTTCCTT ATATGCCAGT TTCAGAAACT 
GTTTCATCTG ATTATGAACA TTCTTCTTCA AGGCATATTT CAAGGAGGAA ACCCTTTGGT 
CAGACTTGCA AAGATGAATC GAGTAGATCA TCAAATGCAC AAAAAGTTCA GCAGGCTCTT 
ACACGCTCTA ACAGTTTATC AACTCCGAGG CCTACAAGAG CTTCCAAACT GCGTCGCGCT 
CGTCTCGGCG AATCTTCTGA CAATGAAAGT GCTGATGTAG AAAGATCAAA TATAAGTCCA 
GGAACGTCTT CTGCAAACTC ATCTTCTGCT AAAAGTTCTA CAGAAACTAA AAAGCCATCT 
CGTTTAGATA TCCTTGCTAT GCCAAGAAAA CGAGCAGGTT CATTTAATGT TCCAAGTGAC 
TCTGAAACAA CTTCTTCAGT AAGAGGAATG TTTTCAGGAC GTAGCATAGA CCAAAGTTAC 
ACTAGTAGAA AACCAGCTGT GGCAGAAAGC AAGCAGCCTC CCAAGAAACC TCTACCACCT 
ACACAGAAGC AAACACCAAG ACCTCGCTCA AGCAGTGTCA AGTACTCATC ATCTTCTACT 
TCACGAAGGA GGCAACAGGG ATCAGATTAT GTTTCTACAT CTGAGGAGGA GTGTGGCTCA 
AATCACAGCA CTCCTAAACA CAAACACTCC CGTGCTTCAA CAGCCACTCA GACTTCAAGA 
TCAAGCAGTG TCTCTCGACG TCAGATGCCC AACAGTCGCC AAGATGAGGA AGAGGATGAG 
CAGGAAGTTT ATAACAACTT CATGGCCCAG TCTGTGGAAA TTGCAGAAAT AGCCAGATTG 
AGTCAAACCC TGGTGAAAGA TGTTGCCAGT TTGGCAAGAG AGATTCATGA TGTAGCAGGA 
GATGGAGATT CTCAGAGTTC CTCAGGAACA GGACAGAGCA CCTCAATCAG TTCTGTGCCA 
AACACCCCAG CCTCAACCAT TTCAGCAAGG GAAGAGATAA TTAAACGGTC CTTACAACGA 
ACGTGCTCAT CACAGTTGGT GCATCATATA CCAGAAGCAA GCCTGAACTA CCAGAAGATT 
CCTCCAGGTT CAACAGGCCT TGAAGACTTT GATCAGAATA TGAATGACAG TCGAGAAGAT 
CCATCAAAGA GAAGAGCTAG GAACATAGAA GAGGTGATTT TTGACAATCT TATGCTGAAT 
CCTGTGTCTC ATTTATCACA TACAATATGC GCAAACACTG AGGTCCTTAC AGAAAAAATG 
AAAATTTTAT TTCAGAATAC AGAGAAGAAC TGGGAAGAAA TTGAAGCAAA GATTAACTCT 
GAAAACGAAG TGCCAATTCT TAAAACATCC AATAAGGAAA TCAGCTCTAT CTTGAAAGAG 
TTGAGAAGGG TTCAGAAACA ATTGGAAGTT ATAAATGCAA TCATCGACCG CTCTGGACAT 
CTAGATGTTC CGTCAAGTAA TAAAAAGACA TCTTCGACTA TCCTGACTTC AAATCCTTTA 
TCTAGGACTA CTAACAATTC AGCTGCCAGA ACAGAGTCTC AAACTCCTGG CCATGTGAGA 
AATTACATGC ATAAATCCAG CTCCAGCTCC TCAAGATCCC CAGGATCCAG TTTCAGTCGG 
GATGACGAAG AAACATATAT TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072723.1
CDS	199..5085
Translation	MSVTSWFLVSSSGTRHRLPREMIFVGREDCELMLQSRSVDKQHAVINYDSDKDEHRVKDLGSLNGTFVNDVRIPDQKYITLKLSDNIRFGYDINTYVLEQIQHQVPEEALKHEKYTSHLQMCLKTAAAGREDQFKEHGAHVDSAQAKQDKADKKATSDIPAYRTPLYGQPSWWGEDDDNKLDKEGRRQDEHYSERPNDMTQHEEEINGNMSYRDSQDQCVYPFRREPSYFEIPTKDFQQPVKPPETQVYEIPTKDTDAVPPVTPPVMQSHASFTIEFDDCQPGKIKIKDHVTKFSMRQRKTAGKEPAPTEMVSAESKVADWLVQNDPSLIRRPAPGEDVYSTKSDLPIHNRTLKGNRHEDGTQSDSEDPLVAQPEQEIGTPDHPQLQRQIKREPEELLHNQQAFVIQFFDDDDAPRKKRSQSFTHNANSPQNDTDPVLKAKAEKRKGTLHVEKVSTNGMGSTAPASKSLSSPSFPQRSNSFRREKTEDRISSAPTTAKLPGKNYGSVGKKSKLAQEFAAEYMREQVEVVKQAAEKPMTLPLSTSILQQPASQVQISSQAQTMQLTSDVRTGKVKNEEEDNLSDAGTYTIETETQDREVEQARKMIDQVFGVFESDEYSKIASTVYRPVIKLGEEEPLASEPSVPHKPIMSSTPPVKLSNGLPAETLIERTGSSSKQSQKWVSRWASLADSYPDASPTSPLDSQKQGLIADDEDVIERTEHQGEADPTVPSRTRRLLPQLPPENVMPTIFVCQESFSDESQRKSLEEPEKRISEENSSLLLVQEELDPDSLSDTSKSDDGVISVRKSAKASNTYRNGWKGEESHSREPSVQRTSVPSNEKKSTCFYVGNDDVGSVKQTSFGLSSKDVIKGPESHIKMKVNLHITAETPSSGKAVNQFPKKDNGSAKDPLSFVRQESFTKETSSSNVLPNKLPHISTHPLLKDLSVTKSNHDYSKETRLILKETETALAALEAKLFTQSHLDEIENTPCLRDDSLSGDSDVDTASTVSLVSDKNVPSHSQKNRIVSLQKEKSSSTSSIQEQYCQPSARERLSEKRRTVPADAGTRNVTKRLGMTRSTGARGSLDFTDEERCSSLPYMPVSETVSSDYEHSSSRHISRRKPFGQTCKDESSRSSNAQKVQQALTRSNSLSTPRPTRASKLRRARLGESSDNESADVERSNISPGTSSANSSSAKSSTETKKPSRLDILAMPRKRAGSFNVPSDSETTSSVRGMFSGRSIDQSYTSRKPAVAESKQPPKKPLPPTQKQTPRPRSSSVKYSSSSTSRRRQQGSDYVSTSEEECGSNHSTPKHKHSRASTATQTSRSSSVSRRQMPNSRQDEEEDEQEVYNNFMAQSVEIAEIARLSQTLVKDVASLAREIHDVAGDGDSQSSSGTGQSTSISSVPNTPASTISAREEIIKRSLQRTCSSQLVHHIPEASLNYQKIPPGSTGLEDFDQNMNDSREDPSKRRARNIEEVIFDNLMLNPVSHLSHTICANTEVLTEKMKILFQNTEKNWEEIEAKINSENEVPILKTSNKEISSILKELRRVQKQLEVINAIIDRSGHLDVPSSNKKTSSTILTSNPLSRTTNNSAARTESQTPGHVRNYMHKSSSSSSRSPGSSFSRDDEETYIV

Target ORF information:

RefSeq Version	NM_001079255.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis centrosomal protein 170B (cep170b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079255.1

ORF Insert Sequence:

ATGAGTGTCA CATCATGGTT CCTTGTCAGC AGCTCTGGCA CGCGCCATCG GCTGCCACGG 
GAAATGATCT TTGTTGGTCG GGAGGATTGT GAGCTTATGC TTCAGTCCCG CAGTGTGGAT 
AAGCAGCATG CAGTAATCAA CTATGACAGT GACAAAGATG AACATCGCGT CAAGGACCTT 
GGGAGCTTAA ATGGGACCTT TGTAAATGAT GTAAGGATTC CTGACCAGAA GTACATTACT 
CTGAAGCTGA GTGACAACAT ACGTTTTGGC TACGATATTA ATACGTATGT ACTGGAGCAG 
ATCCAGCACC AGGTACCAGA AGAGGCACTG AAGCATGAAA AGTACACCAG CCACCTTCAG 
ATGTGTTTGA AAACCGCTGC AGCAGGAAGA GAAGACCAGT TTAAAGAGCA TGGTGCCCAC 
GTAGACTCTG CCCAAGCCAA ACAGGACAAA GCTGACAAGA AAGCTACTAG TGATATTCCT 
GCCTACCGTA CACCTTTGTA CGGTCAACCA TCTTGGTGGG GAGAGGATGA TGATAACAAG 
CTTGATAAGG AAGGCAGAAG ACAAGATGAA CACTATTCAG AAAGGCCAAA CGATATGACT 
CAACATGAGG AAGAAATAAA TGGTAATATG AGCTACAGGG ATTCTCAGGA TCAGTGTGTG 
TACCCATTCA GAAGAGAACC TAGTTACTTT GAAATTCCAA CTAAAGACTT TCAACAGCCT 
GTAAAGCCCC CAGAAACCCA GGTCTATGAA ATCCCTACTA AGGACACAGA TGCAGTGCCC 
CCTGTAACAC CCCCTGTCAT GCAGAGCCAT GCCTCTTTTA CAATTGAGTT TGATGATTGC 
CAGCCTGGAA AAATAAAAAT TAAAGATCAT GTAACAAAAT TTTCTATGAG ACAGAGGAAA 
ACAGCTGGAA AGGAACCTGC TCCAACAGAA ATGGTGTCAG CAGAAAGTAA GGTGGCTGAC 
TGGCTGGTTC AAAATGACCC AAGTTTGATT AGAAGACCAG CTCCTGGAGA AGATGTCTAC 
AGTACCAAGA GTGACCTTCC TATCCATAAC AGAACTCTTA AAGGTAACAG ACATGAAGAT 
GGAACACAGA GTGATTCAGA AGACCCCTTG GTGGCCCAAC CTGAACAGGA AATTGGAACA 
CCTGATCACC CACAGCTCCA GCGGCAGATC AAGCGCGAAC CTGAAGAATT GCTGCACAAT 
CAGCAGGCGT TTGTCATACA GTTTTTTGAT GATGATGATG CCCCTAGGAA AAAACGGTCC 
CAGTCTTTTA CACACAATGC AAACTCCCCT CAGAATGACA CAGACCCAGT CCTTAAAGCA 
AAAGCTGAAA AGCGAAAGGG CACATTGCAT GTTGAAAAAG TGTCAACTAA TGGAATGGGT 
TCAACTGCTC CAGCATCAAA ATCACTGTCA AGCCCTTCAT TCCCTCAAAG ATCAAACTCT 
TTTAGGCGAG AAAAGACAGA GGACCGCATT TCTTCTGCAC CTACGACAGC AAAGTTACCA 
GGGAAGAACT ATGGAAGTGT TGGGAAGAAA TCAAAGCTTG CCCAAGAATT TGCTGCTGAA 
TATATGCGTG AACAAGTAGA GGTAGTTAAA CAGGCAGCAG AAAAACCCAT GACCTTGCCT 
TTGTCTACAT CAATATTGCA GCAACCAGCC TCACAAGTGC AAATCTCAAG CCAAGCACAA 
ACAATGCAGC TAACATCTGA TGTAAGAACA GGAAAAGTTA AAAATGAAGA AGAGGACAAC 
CTAAGTGATG CAGGAACATA CACAATAGAA ACAGAGACAC AGGATAGAGA AGTTGAACAA 
GCCAGGAAAA TGATAGACCA GGTCTTTGGA GTATTTGAAT CCGATGAGTA TTCCAAGATT 
GCATCCACAG TTTATAGGCC AGTTATTAAA TTGGGAGAAG AGGAACCTCT TGCCAGTGAA 
CCAAGTGTAC CACACAAGCC CATAATGTCA TCAACTCCAC CAGTAAAACT AAGTAATGGG 
CTTCCGGCTG AAACTTTGAT TGAAAGAACT GGGTCAAGCT CCAAACAAAG TCAGAAATGG 
GTTTCCCGTT GGGCAAGCCT CGCTGACAGT TATCCCGATG CATCACCAAC ATCTCCTTTG 
GACAGTCAGA AGCAAGGCTT AATTGCAGAT GATGAAGACG TTATTGAGCG TACAGAGCAC 
CAAGGAGAAG CAGATCCCAC TGTGCCATCA AGAACAAGAC GGCTCCTTCC ACAACTTCCT 
CCTGAAAATG TAATGCCAAC AATCTTTGTA TGCCAAGAGT CTTTTTCAGA TGAATCTCAA 
AGGAAGTCTT TGGAAGAACC AGAAAAGAGA ATTTCTGAAG AAAACAGCAG TTTATTATTA 
GTTCAAGAGG AATTAGATCC AGACAGTCTT AGTGATACCA GCAAGTCTGA TGATGGTGTC 
ATATCAGTCA GGAAGTCTGC CAAGGCTTCC AACACTTATA GAAATGGATG GAAGGGAGAG 
GAGTCTCATA GTAGGGAACC ATCTGTTCAG AGAACTTCAG TACCTTCTAA TGAAAAGAAA 
TCAACTTGTT TTTATGTTGG AAATGATGAC GTTGGAAGTG TAAAACAAAC AAGCTTTGGA 
TTGTCCTCTA AAGATGTGAT TAAGGGCCCA GAAAGCCACA TTAAGATGAA AGTTAATTTA 
CACATAACTG CTGAGACTCC AAGCTCAGGA AAGGCAGTTA ATCAGTTTCC CAAGAAAGAT 
AATGGCTCTG CAAAAGATCC TCTTTCATTT GTAAGGCAGG AAAGCTTTAC CAAAGAGACA 
TCCAGTAGCA ATGTTTTACC AAATAAGCTT CCACATATTT CAACTCATCC ATTGCTTAAA 
GATTTAAGTG TTACAAAATC AAATCATGAC TACAGTAAGG AGACACGCCT AATACTTAAA 
GAGACAGAAA CCGCTTTGGC TGCTTTAGAA GCTAAGCTCT TTACACAGTC CCACCTTGAT 
GAAATAGAAA ATACTCCTTG TTTGCGTGAT GACTCCTTAT CAGGAGACTC TGATGTGGAT 
ACAGCCAGCA CAGTTAGTCT AGTCAGTGAT AAAAATGTAC CAAGTCATTC ACAGAAAAAC 
AGAATTGTCA GCCTTCAGAA AGAAAAGTCT TCCTCCACTT CTTCTATACA AGAGCAATAT 
TGTCAACCAA GTGCTCGAGA ACGTCTTTCT GAGAAACGAA GGACAGTTCC TGCTGATGCT 
GGAACTAGAA ATGTAACCAA ACGACTAGGG ATGACACGTA GCACTGGAGC ACGTGGCTCA 
TTAGACTTCA CAGATGAAGA AAGGTGTTCT AGTCTTCCTT ATATGCCAGT TTCAGAAACT 
GTTTCATCTG ATTATGAACA TTCTTCTTCA AGGCATATTT CAAGGAGGAA ACCCTTTGGT 
CAGACTTGCA AAGATGAATC GAGTAGATCA TCAAATGCAC AAAAAGTTCA GCAGGCTCTT 
ACACGCTCTA ACAGTTTATC AACTCCGAGG CCTACAAGAG CTTCCAAACT GCGTCGCGCT 
CGTCTCGGCG AATCTTCTGA CAATGAAAGT GCTGATGTAG AAAGATCAAA TATAAGTCCA 
GGAACGTCTT CTGCAAACTC ATCTTCTGCT AAAAGTTCTA CAGAAACTAA AAAGCCATCT 
CGTTTAGATA TCCTTGCTAT GCCAAGAAAA CGAGCAGGTT CATTTAATGT TCCAAGTGAC 
TCTGAAACAA CTTCTTCAGT AAGAGGAATG TTTTCAGGAC GTAGCATAGA CCAAAGTTAC 
ACTAGTAGAA AACCAGCTGT GGCAGAAAGC AAGCAGCCTC CCAAGAAACC TCTACCACCT 
ACACAGAAGC AAACACCAAG ACCTCGCTCA AGCAGTGTCA AGTACTCATC ATCTTCTACT 
TCACGAAGGA GGCAACAGGG ATCAGATTAT GTTTCTACAT CTGAGGAGGA GTGTGGCTCA 
AATCACAGCA CTCCTAAACA CAAACACTCC CGTGCTTCAA CAGCCACTCA GACTTCAAGA 
TCAAGCAGTG TCTCTCGACG TCAGATGCCC AACAGTCGCC AAGATGAGGA AGAGGATGAG 
CAGGAAGTTT ATAACAACTT CATGGCCCAG TCTGTGGAAA TTGCAGAAAT AGCCAGATTG 
AGTCAAACCC TGGTGAAAGA TGTTGCCAGT TTGGCAAGAG AGATTCATGA TGTAGCAGGA 
GATGGAGATT CTCAGAGTTC CTCAGGAACA GGACAGAGCA CCTCAATCAG TTCTGTGCCA 
AACACCCCAG CCTCAACCAT TTCAGCAAGG GAAGAGATAA TTAAACGGTC CTTACAACGA 
ACGTGCTCAT CACAGTTGGT GCATCATATA CCAGAAGCAA GCCTGAACTA CCAGAAGATT 
CCTCCAGGTT CAACAGGCCT TGAAGACTTT GATCAGAATA TGAATGACAG TCGAGAAGAT 
CCATCAAAGA GAAGAGCTAG GAACATAGAA GAGGTGATTT TTGACAATCT TATGCTGAAT 
CCTGTGTCTC ATTTATCACA TACAATATGC GCAAACACTG AGGTCCTTAC AGAAAAAATG 
AAAATTTTAT TTCAGAATAC AGAGAAGAAC TGGGAAGAAA TTGAAGCAAA GATTAACTCT 
GAAAACGAAG TGCCAATTCT TAAAACATCC AATAAGGAAA TCAGCTCTAT CTTGAAAGAG 
TTGAGAAGGG TTCAGAAACA ATTGGAAGTT ATAAATGCAA TCATCGACCG CTCTGGACAT 
CTAGATGTTC CGTCAAGTAA TAAAAAGACA TCTTCGACTA TCCTGACTTC AAATCCTTTA 
TCTAGGACTA CTAACAATTC AGCTGCCAGA ACAGAGTCTC AAACTCCTGG CCATGTGAGA 
AATTACATGC ATAAATCCAG CTCCAGCTCC TCAAGATCCC CAGGATCCAG TTTCAGTCGG 
GATGACGAAG AAACATATAT TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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cep170b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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