actn1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	actn1
Entrez Gene ID	780123
Full Name	actinin alpha 1
Synonyms	a-actinin,actinin
General protein information	Preferred Names actinin alpha 1 Names alpha-actinin-1 alpha actinin
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012968674.3	XP_012824128.1	alpha-actinin-1 isoform X1
XM_012968675.2	XP_012824129.1	alpha-actinin-1 isoform X2
XM_012968675.3	XP_012824129.1	alpha-actinin-1 isoform X2
XM_012968674.2	XP_012824128.1	alpha-actinin-1 isoform X1
NM_001079198.1	NP_001072666.1	alpha-actinin-1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ACTN1	XP_001139826.3
Mus musculus (house mouse)	Actn1	NP_598917.1
Homo sapiens (human)	ACTN1	NP_001123476.1
Canis lupus familiaris (dog)	ACTN1	XP_853103.1
Bos taurus (cattle)	ACTN1	NP_001030428.1
Xenopus tropicalis (tropical clawed frog)	actn1	NP_001072666.1
Rattus norvegicus (Norway rat)	Actn1	NP_112267.1
Gallus gallus (chicken)	ACTN1	NP_989458.1
Danio rerio (zebrafish)	actn1	NP_001161758.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following actn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the actn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa21223	NM_001079198.1 Latest version!	Xenopus tropicalis actinin alpha 1 (actn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa29489	XM_012968674.2 Latest version!	Xenopus tropicalis actinin, alpha 1 (actn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa29490	XM_012968675.2 Latest version!	Xenopus tropicalis actinin, alpha 1 (actn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa63813	XM_012968674.3 Latest version!	Xenopus tropicalis actinin alpha 1 (actn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa63814	XM_012968675.3 Latest version!	Xenopus tropicalis actinin alpha 1 (actn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

actn1 ( NM_001079198.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001072666.1 Xenopus tropicalis actinin alpha 1 (actn1), mRNA.

Price & Availability↑

CloneID	OXa21223
Clone ID Related Accession (Same CDS sequence)	NM_001079198.1
Accession Version	NM_001079198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	alpha-actinin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC123984.1. ##Evidence-Data-START## Transcript exon combination :: BC123984.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGATCATT ATGACTCCCA GAATAACTAT ATGCAGCAGG AAGACGATTG GGACAGGGAC 
CTCCTGTTGG ATCCAGCCTG GGAGAAGCAG CAAAGAAAGA CATTTACAGC ATGGTGCAAC 
TCACATCTCC GGAAAGCTGG GACACAGATT GAGAACATAG ATGAGGATTT CAGGGATGGC 
CTTAAACTCA TGTTATTACT TGAAGTCATT TCAGGTGAGC GTTTGGCCAA ACCTGAACGA 
GGCAAGATGC GTGTACACAA AATCTCCAAT GTCAATAAAG CCCTGGATTT TATTGCCAGT 
AAAGGTGTAA AACTGGTATC CATTGGAGCC GAAGAAATTG TGGATGGCAA TGCCAAGATG 
ACATTGGGAA TGATCTGGAC AATTATCCTG CGTTTTGCTA TCCAAGACAT TTCTGTTGAA 
GAAACATCTG CCAAAGAAGG TTTACTGCTG TGGTGTCAAA GAAAGACTGC ACCGTACAAG 
AATGTGAATA TCCAGAACTT CCACATAAGC TGGAAAGATG GCCTTGGTTT CTGTGCCCTT 
ATACATCGGC ATCGCCCGGA ACTTATAGAC TACGGCAAAT TGCGCAAGGA CGATCCTCTC 
ACAAATCTAA ACACTGCTTT TGATGTTGCT GAGCGTTACC TGGACATCCC TAAGATGTTA 
GATGCTGAAG ACATTGTTGG AACAGCCCGC CCCGATGAGA AGGCCATCAT GACCTATGTT 
TCTAGCTTCT ACCACGCCTT TTCAGGAGCC CAGAAGGCAG AAACCGCTGC CAACCGCATC 
TGCAAAGTGC TGGCTGTCAA TCAGGAAAAT GAGCAGCTCA TGGAAGATTA TGAAAAGCTG 
GCCAGTGATC TGCTGGAGTG GATCCGCCGC ACAATCCCTT GGTTAGAAAA CAGGGCCCCA 
GAAAATACTA TGCAAGCCAT GCAGCAAAAG CTCGAGGATT TCCGGGACTA TCGCCGTTTG 
CATAAACCCC CTAAGGTTCA AGAGAAATGT CAACTAGAGA TCAACTTTAA CACCCTCCAA 
ACCAAGCTGA GGCTCAGCAA CCGCCCTGCA TTTATGCCTT CTGAGGGGAA GATGGTCTCG 
GACATAAATA ATGCATGGGG AGGATTGGAG CAAGCTGAGA AGGGTTATGA GGAGTGGATG 
TTGAATGAAA TCCGGAGGTT GGAAAGACTA GACCATTTAG CAGAGAAGTT CCGCCAGAAA 
GCATCTATTC ATGAAGCTTG GACTGATGGT AAAGAAGCCA TGCTGCAGAA GAAGGACTAT 
GAGACTGCAA GTCTGTCTGA TATCAAGGCT CTTCTGAAGA AACATGAGGC CTTTGAAAGT 
GACCTGGCTG CCCATCAAGA CAGAGTAGAA CAGATTGCAG CAATAGCACA GGAGCTTAAT 
GAGCTGGACT ACTATGACTC GCAAACTGTT AATGCCAGGT GCCAAAGAAT TTGTGACCAG 
TGGGATAACC TCGGAGCTCT GACACAGCAA AGAAGGGAAG CTTTAGAGAG AACTGAGAAG 
TTGCTAGAAA CCATTGATCA GCTGTACTTA GAATATGCCA AGCGAGCTGC ACCCTTCAAC 
AACTGGATGG AAGGAGCAAT GGAAGACTTA CAGGACACCT TCATTGTCCA TACCATAGAG 
GAGATTCAGG GATTAACCAC AGCGCATGAG CAATTCAAGG CAACATTACC TGAGGCTGAC 
AAAGAGCGCC AAGCAATCCT AGGAATTCAG AATGAGATAT CCAAGATCAT GCAGACGTAT 
CATGTCAATA TGTCTGGCTC AAACCCCTAC ACCAGCATTA CACCTCAAGA AATCAACAAC 
AAATGGGACC ATGTCAGACA GCTGGTCCCC CGTCGGGATC AGGCACTTAC AGAGGAACAT 
GCCCGGCAGC AGCAGAACGA GCGACTGCGG AAACAGTTTG CTGCACAGGC CAACGTCATT 
GGACCATGGA TACAAACAAA GATGCAGGAG ATCGGCCGCA TTGCTATAGA AATGCATGGC 
ACCCTGGAGG ACCAATTGAA TAATCTACGG CAGTATGAGA AAAGCATTGT AAACTACAAA 
CCAAAGATTG ACCAGTTAGA GAGTGACCAC CAACAGATTC AGGAAGCACT TATTTTTGAC 
AACAAACACA CCAACTACAC CATGGAGCAC ATTCGCGTGG GCTGGGAGCA GCTTCTAACC 
ACTATTGCCA GGACCATCAA CGAGGTAGAA AATCAGGTCC TCACAAGAGA TGCCAAAGGC 
ATTAGCCAAG AACAAATGAA TGAATTCAGG AACTCATTCA GTCATTTTGA TAGGAAGAAG 
AGTGGTTTCT TGGAGAGTGA TGATTTCCGC GCCTGTCTTA TCTCCATGGG TTACAATATG 
GGTGAAGCAG AATTTGCTCG AATTATGAGC ATTGTTGACC CCAACAGACT TGGGCTTGTA 
ACATTTCAAG CCTTTATTGA CTTCATGTCC CGTGAAACTG CAGACACTGA CACAGCAGAC 
CAAGTTATGG CTTCTTTCAA GATTCTTGCT GGAGATAAGA ATTACATCAC TGCAGATGAA 
CTGCGCCGGG AACTGCCTCC CGATCAAGCA GAATACTGCA TTGCCAGAAT GGCTCCATAC 
CTGGGACCTG ATGCTGTGCC AGGAGCTCTG GACTACATGT CTTTCTCAAC AGCACTATAT 
GGAGAAAGTG ACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072666.1
CDS	28..2685
Translation	MDHYDSQNNYMQQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAERYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRAPENTMQAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNAWGGLEQAEKGYEEWMLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLQKKDYETASLSDIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSQTVNARCQRICDQWDNLGALTQQRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPEADKERQAILGIQNEISKIMQTYHVNMSGSNPYTSITPQEINNKWDHVRQLVPRRDQALTEEHARQQQNERLRKQFAAQANVIGPWIQTKMQEIGRIAIEMHGTLEDQLNNLRQYEKSIVNYKPKIDQLESDHQQIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQVLTRDAKGISQEQMNEFRNSFSHFDRKKSGFLESDDFRACLISMGYNMGEAEFARIMSIVDPNRLGLVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITADELRRELPPDQAEYCIARMAPYLGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	NM_001079198.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis actinin alpha 1 (actn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079198.1

ORF Insert Sequence:

ATGGATCATT ATGACTCCCA GAATAACTAT ATGCAGCAGG AAGACGATTG GGACAGGGAC 
CTCCTGTTGG ATCCAGCCTG GGAGAAGCAG CAAAGAAAGA CATTTACAGC ATGGTGCAAC 
TCACATCTCC GGAAAGCTGG GACACAGATT GAGAACATAG ATGAGGATTT CAGGGATGGC 
CTTAAACTCA TGTTATTACT TGAAGTCATT TCAGGTGAGC GTTTGGCCAA ACCTGAACGA 
GGCAAGATGC GTGTACACAA AATCTCCAAT GTCAATAAAG CCCTGGATTT TATTGCCAGT 
AAAGGTGTAA AACTGGTATC CATTGGAGCC GAAGAAATTG TGGATGGCAA TGCCAAGATG 
ACATTGGGAA TGATCTGGAC AATTATCCTG CGTTTTGCTA TCCAAGACAT TTCTGTTGAA 
GAAACATCTG CCAAAGAAGG TTTACTGCTG TGGTGTCAAA GAAAGACTGC ACCGTACAAG 
AATGTGAATA TCCAGAACTT CCACATAAGC TGGAAAGATG GCCTTGGTTT CTGTGCCCTT 
ATACATCGGC ATCGCCCGGA ACTTATAGAC TACGGCAAAT TGCGCAAGGA CGATCCTCTC 
ACAAATCTAA ACACTGCTTT TGATGTTGCT GAGCGTTACC TGGACATCCC TAAGATGTTA 
GATGCTGAAG ACATTGTTGG AACAGCCCGC CCCGATGAGA AGGCCATCAT GACCTATGTT 
TCTAGCTTCT ACCACGCCTT TTCAGGAGCC CAGAAGGCAG AAACCGCTGC CAACCGCATC 
TGCAAAGTGC TGGCTGTCAA TCAGGAAAAT GAGCAGCTCA TGGAAGATTA TGAAAAGCTG 
GCCAGTGATC TGCTGGAGTG GATCCGCCGC ACAATCCCTT GGTTAGAAAA CAGGGCCCCA 
GAAAATACTA TGCAAGCCAT GCAGCAAAAG CTCGAGGATT TCCGGGACTA TCGCCGTTTG 
CATAAACCCC CTAAGGTTCA AGAGAAATGT CAACTAGAGA TCAACTTTAA CACCCTCCAA 
ACCAAGCTGA GGCTCAGCAA CCGCCCTGCA TTTATGCCTT CTGAGGGGAA GATGGTCTCG 
GACATAAATA ATGCATGGGG AGGATTGGAG CAAGCTGAGA AGGGTTATGA GGAGTGGATG 
TTGAATGAAA TCCGGAGGTT GGAAAGACTA GACCATTTAG CAGAGAAGTT CCGCCAGAAA 
GCATCTATTC ATGAAGCTTG GACTGATGGT AAAGAAGCCA TGCTGCAGAA GAAGGACTAT 
GAGACTGCAA GTCTGTCTGA TATCAAGGCT CTTCTGAAGA AACATGAGGC CTTTGAAAGT 
GACCTGGCTG CCCATCAAGA CAGAGTAGAA CAGATTGCAG CAATAGCACA GGAGCTTAAT 
GAGCTGGACT ACTATGACTC GCAAACTGTT AATGCCAGGT GCCAAAGAAT TTGTGACCAG 
TGGGATAACC TCGGAGCTCT GACACAGCAA AGAAGGGAAG CTTTAGAGAG AACTGAGAAG 
TTGCTAGAAA CCATTGATCA GCTGTACTTA GAATATGCCA AGCGAGCTGC ACCCTTCAAC 
AACTGGATGG AAGGAGCAAT GGAAGACTTA CAGGACACCT TCATTGTCCA TACCATAGAG 
GAGATTCAGG GATTAACCAC AGCGCATGAG CAATTCAAGG CAACATTACC TGAGGCTGAC 
AAAGAGCGCC AAGCAATCCT AGGAATTCAG AATGAGATAT CCAAGATCAT GCAGACGTAT 
CATGTCAATA TGTCTGGCTC AAACCCCTAC ACCAGCATTA CACCTCAAGA AATCAACAAC 
AAATGGGACC ATGTCAGACA GCTGGTCCCC CGTCGGGATC AGGCACTTAC AGAGGAACAT 
GCCCGGCAGC AGCAGAACGA GCGACTGCGG AAACAGTTTG CTGCACAGGC CAACGTCATT 
GGACCATGGA TACAAACAAA GATGCAGGAG ATCGGCCGCA TTGCTATAGA AATGCATGGC 
ACCCTGGAGG ACCAATTGAA TAATCTACGG CAGTATGAGA AAAGCATTGT AAACTACAAA 
CCAAAGATTG ACCAGTTAGA GAGTGACCAC CAACAGATTC AGGAAGCACT TATTTTTGAC 
AACAAACACA CCAACTACAC CATGGAGCAC ATTCGCGTGG GCTGGGAGCA GCTTCTAACC 
ACTATTGCCA GGACCATCAA CGAGGTAGAA AATCAGGTCC TCACAAGAGA TGCCAAAGGC 
ATTAGCCAAG AACAAATGAA TGAATTCAGG AACTCATTCA GTCATTTTGA TAGGAAGAAG 
AGTGGTTTCT TGGAGAGTGA TGATTTCCGC GCCTGTCTTA TCTCCATGGG TTACAATATG 
GGTGAAGCAG AATTTGCTCG AATTATGAGC ATTGTTGACC CCAACAGACT TGGGCTTGTA 
ACATTTCAAG CCTTTATTGA CTTCATGTCC CGTGAAACTG CAGACACTGA CACAGCAGAC 
CAAGTTATGG CTTCTTTCAA GATTCTTGCT GGAGATAAGA ATTACATCAC TGCAGATGAA 
CTGCGCCGGG AACTGCCTCC CGATCAAGCA GAATACTGCA TTGCCAGAAT GGCTCCATAC 
CTGGGACCTG ATGCTGTGCC AGGAGCTCTG GACTACATGT CTTTCTCAAC AGCACTATAT 
GGAGAAAGTG ACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

actn1 ( XM_012968674.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824128.1 Xenopus tropicalis actinin, alpha 1 (actn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa29489
Clone ID Related Accession (Same CDS sequence)	XM_012968674.2
Accession Version	XM_012968674.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	alpha-actinin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968674.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCATT ATGACTCCCA GAATAACTAT ATGCAGCAGG AAGACGATTG GGACAGGGAC 
CTCCTGTTGG ATCCAGCCTG GGAGAAGCAG CAAAGAAAGA CATTTACAGC ATGGTGCAAC 
TCACATCTCC GGAAAGCTGG GACACAGATT GAGAACATAG ATGAGGATTT CAGGGATGGC 
CTTAAACTCA TGTTATTACT TGAAGTCATT TCAGGTGAGC GTTTGGCCAA ACCTGAACGA 
GGCAAGATGC GTGTACACAA AATCTCCAAT GTCAATAAAG CCCTGGATTT TATTGCCAGT 
AAAGGTGTAA AACTGGTATC CATTGGAGCC GAAGAAATTG TGGATGGCAA TGCAAAGATG 
ACATTGGGAA TGATCTGGAC AATTATCCTG CGTTTTGCTA TCCAAGACAT TTCTGTTGAA 
GAAACATCTG CCAAAGAAGG TTTACTGCTG TGGTGTCAAA GAAAGACTGC ACCGTACAAG 
AATGTGAATA TTCAGAACTT CCACATAAGC TGGAAAGATG GCCTTGGTTT CTGTGCCCTT 
ATACATCGGC ATCGCCCGGA ACTTATAGAC TACGGCAAAT TGCGCAAGGA CGATCCTCTC 
ACAAATCTAA ACACTGCTTT TGATGTTGCT GAGCGTTACC TGGACATCCC TAAGATGTTA 
GATGCTGAAG ACATTGTTGG AACAGCCCGC CCCGATGAGA AGGCCATCAT GACCTATGTT 
TCTAGCTTCT ACCACGCCTT TTCAGGAGCC CAGAAGGCAG AAACCGCTGC CAACCGCATC 
TGCAAAGTGC TGGCTGTCAA TCAGGAAAAT GAGCAGCTCA TGGAAGATTA TGAAAAGCTG 
GCCAGTGATC TGCTGGAGTG GATCCGCCGC ACAATCCCTT GGTTAGAAAA CAGGGCCCCA 
GAAAACACTA TGCAAGCCAT GCAGCAAAAG CTCGAGGATT TCCGGGACTA TCGCCGTTTG 
CATAAACCCC CTAAGGTTCA AGAGAAATGT CAACTAGAGA TCAACTTTAA CACCCTCCAA 
ACCAAGCTGA GGCTCAGCAA CCGCCCTGCA TTTATGCCTT CTGAGGGGAA GATGGTCTCG 
GACATAAATA ATGCATGGGG AGGATTGGAG CAAGCTGAGA AGGGTTATGA GGAGTGGATG 
TTGAATGAAA TCCGGAGGTT GGAAAGACTA GACCATTTAG CAGAGAAGTT CCGCCAGAAA 
GCATCTATTC ATGAAGCTTG GACTGATGGT AAAGAAGCCA TGCTGCAGAA GAAGGACTAT 
GAGACTGCAA GTCTGTCTGA TATCAAGGCT CTTCTGAAGA AACATGAGGC CTTTGAAAGT 
GACCTGGCTG CCCATCAAGA CAGAGTAGAA CAGATTGCAG CAATAGCACA GGAGCTTAAT 
GAGCTGGACT ACTATGACTC GCAAACTGTT AATGCCAGGT GCCAAAGAAT TTGTGACCAG 
TGGGATAACC TCGGAGCTCT GACACAGCAA AGAAGGGAAG CTTTAGAGAG AACTGAGAAG 
TTGCTAGAAA CCATTGATCA GCTGTACTTA GAATATGCCA AGCGAGCTGC ACCCTTCAAC 
AACTGGATGG AAGGAGCAAT GGAAGACTTA CAGGACACCT TCATTGTCCA TACCATAGAG 
GAGATTCAGG GATTAACCAC AGCGCATGAG CAATTCAAGG CAACATTACC TGAGGCTGAC 
AAAGAGCGCC AAGCAATCCT AGGAATTCAG AATGAGATAT CCAAGATCAT GCAGACGTAT 
CATGTCAATA TGTCTGGCTC AAACCCCTAC ACCAGCATTA CACCTCAAGA AATCAACAAC 
AAATGGGACC ATGTCAGACA GCTGGTCCCC CGTCGGGATC AGGCACTTAC AGAGGAACAT 
GCCCGGCAGC AGCAGAACGA GCGACTGCGG AAACAGTTTG CTGCACAGGC CAACGTCATT 
GGACCATGGA TACAAACAAA GATGCAGGAG ATCGGCCGCA TTGCTATAGA AATGCATGGC 
ACCCTGGAGG ACCAATTGAA TAATCTACGG CAGTATGAGA AAAGCATTGT AAACTACAAA 
CCAAAGATTG ACCAGTTAGA GAGTGACCAC CAACAGATTC AGGAAGCACT TATTTTTGAC 
AACAAACACA CCAACTACAC CATGGAGCAC ATTCGCGTGG GCTGGGAGCA GCTTCTAACC 
ACTATTGCCA GGACCATCAA CGAGGTAGAA AATCAGGTCC TCACAAGAGA TGCCAAAGGC 
ATTAGCCAAG AACAAATGAA TGAATTCAGG AACTCATTCA GTCATTTTGA TAGGGATCAC 
TCTGGCAGGC TTGGTCCGGA AGAGTTTAAA GCTTGCCTCA TCAGCTTGGG TTACGATATT 
GGAAACGATC CTCAGAAGAA GAGTGGTTTC TTGGAGAGTG ATGATTTCCG CGCCTGTCTT 
ATCTCCATGG GTTACAATAT GGGTGAAGCA GAATTTGCTC GAATTATGAG CATTGTTGAC 
CCCAACAGAC TTGGGCTTGT AACATTTCAA GCCTTTATTG ACTTCATGTC CCGTGAAACT 
GCAGACACTG ACACAGCAGA CCAAGTTATG GCTTCTTTCA AGATTCTTGC TGGAGATAAG 
AATTACATCA CTGCAGATGA ACTGCGCCGG GAACTGCCTC CCGATCAAGC AGAATACTGC 
ATTGCCAGAA TGGCTCCATA CCTGGGACCT GATGCTGTGC CAGGAGCTCT GGACTACATG 
TCTTTCTCAA CAGCACTATA TGGAGAAAGT GACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824128.1
CDS	157..2895
Translation	MDHYDSQNNYMQQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAERYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRAPENTMQAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNAWGGLEQAEKGYEEWMLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLQKKDYETASLSDIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSQTVNARCQRICDQWDNLGALTQQRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPEADKERQAILGIQNEISKIMQTYHVNMSGSNPYTSITPQEINNKWDHVRQLVPRRDQALTEEHARQQQNERLRKQFAAQANVIGPWIQTKMQEIGRIAIEMHGTLEDQLNNLRQYEKSIVNYKPKIDQLESDHQQIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQVLTRDAKGISQEQMNEFRNSFSHFDRDHSGRLGPEEFKACLISLGYDIGNDPQKKSGFLESDDFRACLISMGYNMGEAEFARIMSIVDPNRLGLVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITADELRRELPPDQAEYCIARMAPYLGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_012968674.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis actinin, alpha 1 (actn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968674.2

ORF Insert Sequence:

ATGGATCATT ATGACTCCCA GAATAACTAT ATGCAGCAGG AAGACGATTG GGACAGGGAC 
CTCCTGTTGG ATCCAGCCTG GGAGAAGCAG CAAAGAAAGA CATTTACAGC ATGGTGCAAC 
TCACATCTCC GGAAAGCTGG GACACAGATT GAGAACATAG ATGAGGATTT CAGGGATGGC 
CTTAAACTCA TGTTATTACT TGAAGTCATT TCAGGTGAGC GTTTGGCCAA ACCTGAACGA 
GGCAAGATGC GTGTACACAA AATCTCCAAT GTCAATAAAG CCCTGGATTT TATTGCCAGT 
AAAGGTGTAA AACTGGTATC CATTGGAGCC GAAGAAATTG TGGATGGCAA TGCAAAGATG 
ACATTGGGAA TGATCTGGAC AATTATCCTG CGTTTTGCTA TCCAAGACAT TTCTGTTGAA 
GAAACATCTG CCAAAGAAGG TTTACTGCTG TGGTGTCAAA GAAAGACTGC ACCGTACAAG 
AATGTGAATA TTCAGAACTT CCACATAAGC TGGAAAGATG GCCTTGGTTT CTGTGCCCTT 
ATACATCGGC ATCGCCCGGA ACTTATAGAC TACGGCAAAT TGCGCAAGGA CGATCCTCTC 
ACAAATCTAA ACACTGCTTT TGATGTTGCT GAGCGTTACC TGGACATCCC TAAGATGTTA 
GATGCTGAAG ACATTGTTGG AACAGCCCGC CCCGATGAGA AGGCCATCAT GACCTATGTT 
TCTAGCTTCT ACCACGCCTT TTCAGGAGCC CAGAAGGCAG AAACCGCTGC CAACCGCATC 
TGCAAAGTGC TGGCTGTCAA TCAGGAAAAT GAGCAGCTCA TGGAAGATTA TGAAAAGCTG 
GCCAGTGATC TGCTGGAGTG GATCCGCCGC ACAATCCCTT GGTTAGAAAA CAGGGCCCCA 
GAAAACACTA TGCAAGCCAT GCAGCAAAAG CTCGAGGATT TCCGGGACTA TCGCCGTTTG 
CATAAACCCC CTAAGGTTCA AGAGAAATGT CAACTAGAGA TCAACTTTAA CACCCTCCAA 
ACCAAGCTGA GGCTCAGCAA CCGCCCTGCA TTTATGCCTT CTGAGGGGAA GATGGTCTCG 
GACATAAATA ATGCATGGGG AGGATTGGAG CAAGCTGAGA AGGGTTATGA GGAGTGGATG 
TTGAATGAAA TCCGGAGGTT GGAAAGACTA GACCATTTAG CAGAGAAGTT CCGCCAGAAA 
GCATCTATTC ATGAAGCTTG GACTGATGGT AAAGAAGCCA TGCTGCAGAA GAAGGACTAT 
GAGACTGCAA GTCTGTCTGA TATCAAGGCT CTTCTGAAGA AACATGAGGC CTTTGAAAGT 
GACCTGGCTG CCCATCAAGA CAGAGTAGAA CAGATTGCAG CAATAGCACA GGAGCTTAAT 
GAGCTGGACT ACTATGACTC GCAAACTGTT AATGCCAGGT GCCAAAGAAT TTGTGACCAG 
TGGGATAACC TCGGAGCTCT GACACAGCAA AGAAGGGAAG CTTTAGAGAG AACTGAGAAG 
TTGCTAGAAA CCATTGATCA GCTGTACTTA GAATATGCCA AGCGAGCTGC ACCCTTCAAC 
AACTGGATGG AAGGAGCAAT GGAAGACTTA CAGGACACCT TCATTGTCCA TACCATAGAG 
GAGATTCAGG GATTAACCAC AGCGCATGAG CAATTCAAGG CAACATTACC TGAGGCTGAC 
AAAGAGCGCC AAGCAATCCT AGGAATTCAG AATGAGATAT CCAAGATCAT GCAGACGTAT 
CATGTCAATA TGTCTGGCTC AAACCCCTAC ACCAGCATTA CACCTCAAGA AATCAACAAC 
AAATGGGACC ATGTCAGACA GCTGGTCCCC CGTCGGGATC AGGCACTTAC AGAGGAACAT 
GCCCGGCAGC AGCAGAACGA GCGACTGCGG AAACAGTTTG CTGCACAGGC CAACGTCATT 
GGACCATGGA TACAAACAAA GATGCAGGAG ATCGGCCGCA TTGCTATAGA AATGCATGGC 
ACCCTGGAGG ACCAATTGAA TAATCTACGG CAGTATGAGA AAAGCATTGT AAACTACAAA 
CCAAAGATTG ACCAGTTAGA GAGTGACCAC CAACAGATTC AGGAAGCACT TATTTTTGAC 
AACAAACACA CCAACTACAC CATGGAGCAC ATTCGCGTGG GCTGGGAGCA GCTTCTAACC 
ACTATTGCCA GGACCATCAA CGAGGTAGAA AATCAGGTCC TCACAAGAGA TGCCAAAGGC 
ATTAGCCAAG AACAAATGAA TGAATTCAGG AACTCATTCA GTCATTTTGA TAGGGATCAC 
TCTGGCAGGC TTGGTCCGGA AGAGTTTAAA GCTTGCCTCA TCAGCTTGGG TTACGATATT 
GGAAACGATC CTCAGAAGAA GAGTGGTTTC TTGGAGAGTG ATGATTTCCG CGCCTGTCTT 
ATCTCCATGG GTTACAATAT GGGTGAAGCA GAATTTGCTC GAATTATGAG CATTGTTGAC 
CCCAACAGAC TTGGGCTTGT AACATTTCAA GCCTTTATTG ACTTCATGTC CCGTGAAACT 
GCAGACACTG ACACAGCAGA CCAAGTTATG GCTTCTTTCA AGATTCTTGC TGGAGATAAG 
AATTACATCA CTGCAGATGA ACTGCGCCGG GAACTGCCTC CCGATCAAGC AGAATACTGC 
ATTGCCAGAA TGGCTCCATA CCTGGGACCT GATGCTGTGC CAGGAGCTCT GGACTACATG 
TCTTTCTCAA CAGCACTATA TGGAGAAAGT GACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

actn1 ( XM_012968675.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824129.1 Xenopus tropicalis actinin, alpha 1 (actn1), transcript variant X2, mRNA.

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CloneID	OXa29490
Clone ID Related Accession (Same CDS sequence)	XM_012968675.2
Accession Version	XM_012968675.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	alpha-actinin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968675.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCATT ATGACTCCCA GAATAACTAT ATGCAGCAGG AAGACGATTG GGACAGGGAC 
CTCCTGTTGG ATCCAGCCTG GGAGAAGCAG CAAAGAAAGA CATTTACAGC ATGGTGCAAC 
TCACATCTCC GGAAAGCTGG GACACAGATT GAGAACATAG ATGAGGATTT CAGGGATGGC 
CTTAAACTCA TGTTATTACT TGAAGTCATT TCAGGTGAGC GTTTGGCCAA ACCTGAACGA 
GGCAAGATGC GTGTACACAA AATCTCCAAT GTCAATAAAG CCCTGGATTT TATTGCCAGT 
AAAGGTGTAA AACTGGTATC CATTGGAGCC GAAGAAATTG TGGATGGCAA TGCAAAGATG 
ACATTGGGAA TGATCTGGAC AATTATCCTG CGTTTTGCTA TCCAAGACAT TTCTGTTGAA 
GAAACATCTG CCAAAGAAGG TTTACTGCTG TGGTGTCAAA GAAAGACTGC ACCGTACAAG 
AATGTGAATA TTCAGAACTT CCACATAAGC TGGAAAGATG GCCTTGGTTT CTGTGCCCTT 
ATACATCGGC ATCGCCCGGA ACTTATAGAC TACGGCAAAT TGCGCAAGGA CGATCCTCTC 
ACAAATCTAA ACACTGCTTT TGATGTTGCT GAGCGTTACC TGGACATCCC TAAGATGTTA 
GATGCTGAAG ACATTGTTGG AACAGCCCGC CCCGATGAGA AGGCCATCAT GACCTATGTT 
TCTAGCTTCT ACCACGCCTT TTCAGGAGCC CAGAAGGCAG AAACCGCTGC CAACCGCATC 
TGCAAAGTGC TGGCTGTCAA TCAGGAAAAT GAGCAGCTCA TGGAAGATTA TGAAAAGCTG 
GCCAGTGATC TGCTGGAGTG GATCCGCCGC ACAATCCCTT GGTTAGAAAA CAGGGCCCCA 
GAAAACACTA TGCAAGCCAT GCAGCAAAAG CTCGAGGATT TCCGGGACTA TCGCCGTTTG 
CATAAACCCC CTAAGGTTCA AGAGAAATGT CAACTAGAGA TCAACTTTAA CACCCTCCAA 
ACCAAGCTGA GGCTCAGCAA CCGCCCTGCA TTTATGCCTT CTGAGGGGAA GATGGTCTCG 
GACATAAATA ATGCATGGGG AGGATTGGAG CAAGCTGAGA AGGGTTATGA GGAGTGGATG 
TTGAATGAAA TCCGGAGGTT GGAAAGACTA GACCATTTAG CAGAGAAGTT CCGCCAGAAA 
GCATCTATTC ATGAAGCTTG GACTGATGGT AAAGAAGCCA TGCTGCAGAA GAAGGACTAT 
GAGACTGCAA GTCTGTCTGA TATCAAGGCT CTTCTGAAGA AACATGAGGC CTTTGAAAGT 
GACCTGGCTG CCCATCAAGA CAGAGTAGAA CAGATTGCAG CAATAGCACA GGAGCTTAAT 
GAGCTGGACT ACTATGACTC GCAAACTGTT AATGCCAGGT GCCAAAGAAT TTGTGACCAG 
TGGGATAACC TCGGAGCTCT GACACAGCAA AGAAGGGAAG CTTTAGAGAG AACTGAGAAG 
TTGCTAGAAA CCATTGATCA GCTGTACTTA GAATATGCCA AGCGAGCTGC ACCCTTCAAC 
AACTGGATGG AAGGAGCAAT GGAAGACTTA CAGGACACCT TCATTGTCCA TACCATAGAG 
GAGATTCAGG GATTAACCAC AGCGCATGAG CAATTCAAGG CAACATTACC TGAGGCTGAC 
AAAGAGCGCC AAGCAATCCT AGGAATTCAG AATGAGATAT CCAAGATCAT GCAGACGTAT 
CATGTCAATA TGTCTGGCTC AAACCCCTAC ACCAGCATTA CACCTCAAGA AATCAACAAC 
AAATGGGACC ATGTCAGACA GCTGGTCCCC CGTCGGGATC AGGCACTTAC AGAGGAACAT 
GCCCGGCAGC AGCAGAACGA GCGACTGCGG AAACAGTTTG CTGCACAGGC CAACGTCATT 
GGACCATGGA TACAAACAAA GATGCAGGAG ATCGGCCGCA TTGCTATAGA AATGCATGGC 
ACCCTGGAGG ACCAATTGAA TAATCTACGG CAGTATGAGA AAAGCATTGT AAACTACAAA 
CCAAAGATTG ACCAGTTAGA GAGTGACCAC CAACAGATTC AGGAAGCACT TATTTTTGAC 
AACAAACACA CCAACTACAC CATGGAGCAC ATTCGCGTGG GCTGGGAGCA GCTTCTAACC 
ACTATTGCCA GGACCATCAA CGAGGTAGAA AATCAGGTCC TCACAAGAGA TGCCAAAGGC 
ATTAGCCAAG AACAAATGAA TGAATTCAGG AACTCATTCA GTCATTTTGA TAGGGATCAC 
TCTGGCAGGC TTGGTCCGGA AGAGTTTAAA GCTTGCCTCA TCAGCTTGGG TTACGATATT 
GGAAACGATC CTCAGGGTGA AGCAGAATTT GCTCGAATTA TGAGCATTGT TGACCCCAAC 
AGACTTGGGC TTGTAACATT TCAAGCCTTT ATTGACTTCA TGTCCCGTGA AACTGCAGAC 
ACTGACACAG CAGACCAAGT TATGGCTTCT TTCAAGATTC TTGCTGGAGA TAAGAATTAC 
ATCACTGCAG ATGAACTGCG CCGGGAACTG CCTCCCGATC AAGCAGAATA CTGCATTGCC 
AGAATGGCTC CATACCTGGG ACCTGATGCT GTGCCAGGAG CTCTGGACTA CATGTCTTTC 
TCAACAGCAC TATATGGAGA AAGTGACCTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824129.1
CDS	157..2829
Translation	MDHYDSQNNYMQQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAERYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRAPENTMQAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNAWGGLEQAEKGYEEWMLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLQKKDYETASLSDIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSQTVNARCQRICDQWDNLGALTQQRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPEADKERQAILGIQNEISKIMQTYHVNMSGSNPYTSITPQEINNKWDHVRQLVPRRDQALTEEHARQQQNERLRKQFAAQANVIGPWIQTKMQEIGRIAIEMHGTLEDQLNNLRQYEKSIVNYKPKIDQLESDHQQIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQVLTRDAKGISQEQMNEFRNSFSHFDRDHSGRLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGLVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITADELRRELPPDQAEYCIARMAPYLGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_012968675.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis actinin, alpha 1 (actn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968675.2

ORF Insert Sequence:

ATGGATCATT ATGACTCCCA GAATAACTAT ATGCAGCAGG AAGACGATTG GGACAGGGAC 
CTCCTGTTGG ATCCAGCCTG GGAGAAGCAG CAAAGAAAGA CATTTACAGC ATGGTGCAAC 
TCACATCTCC GGAAAGCTGG GACACAGATT GAGAACATAG ATGAGGATTT CAGGGATGGC 
CTTAAACTCA TGTTATTACT TGAAGTCATT TCAGGTGAGC GTTTGGCCAA ACCTGAACGA 
GGCAAGATGC GTGTACACAA AATCTCCAAT GTCAATAAAG CCCTGGATTT TATTGCCAGT 
AAAGGTGTAA AACTGGTATC CATTGGAGCC GAAGAAATTG TGGATGGCAA TGCAAAGATG 
ACATTGGGAA TGATCTGGAC AATTATCCTG CGTTTTGCTA TCCAAGACAT TTCTGTTGAA 
GAAACATCTG CCAAAGAAGG TTTACTGCTG TGGTGTCAAA GAAAGACTGC ACCGTACAAG 
AATGTGAATA TTCAGAACTT CCACATAAGC TGGAAAGATG GCCTTGGTTT CTGTGCCCTT 
ATACATCGGC ATCGCCCGGA ACTTATAGAC TACGGCAAAT TGCGCAAGGA CGATCCTCTC 
ACAAATCTAA ACACTGCTTT TGATGTTGCT GAGCGTTACC TGGACATCCC TAAGATGTTA 
GATGCTGAAG ACATTGTTGG AACAGCCCGC CCCGATGAGA AGGCCATCAT GACCTATGTT 
TCTAGCTTCT ACCACGCCTT TTCAGGAGCC CAGAAGGCAG AAACCGCTGC CAACCGCATC 
TGCAAAGTGC TGGCTGTCAA TCAGGAAAAT GAGCAGCTCA TGGAAGATTA TGAAAAGCTG 
GCCAGTGATC TGCTGGAGTG GATCCGCCGC ACAATCCCTT GGTTAGAAAA CAGGGCCCCA 
GAAAACACTA TGCAAGCCAT GCAGCAAAAG CTCGAGGATT TCCGGGACTA TCGCCGTTTG 
CATAAACCCC CTAAGGTTCA AGAGAAATGT CAACTAGAGA TCAACTTTAA CACCCTCCAA 
ACCAAGCTGA GGCTCAGCAA CCGCCCTGCA TTTATGCCTT CTGAGGGGAA GATGGTCTCG 
GACATAAATA ATGCATGGGG AGGATTGGAG CAAGCTGAGA AGGGTTATGA GGAGTGGATG 
TTGAATGAAA TCCGGAGGTT GGAAAGACTA GACCATTTAG CAGAGAAGTT CCGCCAGAAA 
GCATCTATTC ATGAAGCTTG GACTGATGGT AAAGAAGCCA TGCTGCAGAA GAAGGACTAT 
GAGACTGCAA GTCTGTCTGA TATCAAGGCT CTTCTGAAGA AACATGAGGC CTTTGAAAGT 
GACCTGGCTG CCCATCAAGA CAGAGTAGAA CAGATTGCAG CAATAGCACA GGAGCTTAAT 
GAGCTGGACT ACTATGACTC GCAAACTGTT AATGCCAGGT GCCAAAGAAT TTGTGACCAG 
TGGGATAACC TCGGAGCTCT GACACAGCAA AGAAGGGAAG CTTTAGAGAG AACTGAGAAG 
TTGCTAGAAA CCATTGATCA GCTGTACTTA GAATATGCCA AGCGAGCTGC ACCCTTCAAC 
AACTGGATGG AAGGAGCAAT GGAAGACTTA CAGGACACCT TCATTGTCCA TACCATAGAG 
GAGATTCAGG GATTAACCAC AGCGCATGAG CAATTCAAGG CAACATTACC TGAGGCTGAC 
AAAGAGCGCC AAGCAATCCT AGGAATTCAG AATGAGATAT CCAAGATCAT GCAGACGTAT 
CATGTCAATA TGTCTGGCTC AAACCCCTAC ACCAGCATTA CACCTCAAGA AATCAACAAC 
AAATGGGACC ATGTCAGACA GCTGGTCCCC CGTCGGGATC AGGCACTTAC AGAGGAACAT 
GCCCGGCAGC AGCAGAACGA GCGACTGCGG AAACAGTTTG CTGCACAGGC CAACGTCATT 
GGACCATGGA TACAAACAAA GATGCAGGAG ATCGGCCGCA TTGCTATAGA AATGCATGGC 
ACCCTGGAGG ACCAATTGAA TAATCTACGG CAGTATGAGA AAAGCATTGT AAACTACAAA 
CCAAAGATTG ACCAGTTAGA GAGTGACCAC CAACAGATTC AGGAAGCACT TATTTTTGAC 
AACAAACACA CCAACTACAC CATGGAGCAC ATTCGCGTGG GCTGGGAGCA GCTTCTAACC 
ACTATTGCCA GGACCATCAA CGAGGTAGAA AATCAGGTCC TCACAAGAGA TGCCAAAGGC 
ATTAGCCAAG AACAAATGAA TGAATTCAGG AACTCATTCA GTCATTTTGA TAGGGATCAC 
TCTGGCAGGC TTGGTCCGGA AGAGTTTAAA GCTTGCCTCA TCAGCTTGGG TTACGATATT 
GGAAACGATC CTCAGGGTGA AGCAGAATTT GCTCGAATTA TGAGCATTGT TGACCCCAAC 
AGACTTGGGC TTGTAACATT TCAAGCCTTT ATTGACTTCA TGTCCCGTGA AACTGCAGAC 
ACTGACACAG CAGACCAAGT TATGGCTTCT TTCAAGATTC TTGCTGGAGA TAAGAATTAC 
ATCACTGCAG ATGAACTGCG CCGGGAACTG CCTCCCGATC AAGCAGAATA CTGCATTGCC 
AGAATGGCTC CATACCTGGG ACCTGATGCT GTGCCAGGAG CTCTGGACTA CATGTCTTTC 
TCAACAGCAC TATATGGAGA AAGTGACCTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

actn1 ( XM_012968674.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824128.1 Xenopus tropicalis actinin alpha 1 (actn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa63813
Clone ID Related Accession (Same CDS sequence)	XM_012968674.3
Accession Version	XM_012968674.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	alpha-actinin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968674.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCATT ATGACTCCCA GAATAACTAT ATGCAGCAGG AAGACGATTG GGACAGGGAC 
CTCCTGTTGG ATCCAGCCTG GGAGAAGCAG CAAAGAAAGA CATTTACAGC ATGGTGCAAC 
TCACATCTCC GGAAAGCTGG GACACAGATT GAGAACATAG ATGAGGATTT CAGGGATGGC 
CTTAAACTCA TGTTATTACT TGAAGTCATT TCAGGTGAGC GTTTGGCCAA ACCTGAACGA 
GGCAAGATGC GTGTACACAA AATCTCCAAT GTCAATAAAG CCCTGGATTT TATTGCCAGT 
AAAGGTGTAA AACTGGTATC CATTGGAGCC GAAGAAATTG TGGATGGCAA TGCCAAGATG 
ACATTGGGAA TGATCTGGAC AATTATCCTG CGTTTTGCTA TCCAAGACAT TTCTGTTGAA 
GAAACATCTG CCAAAGAAGG TTTACTGCTG TGGTGTCAAA GAAAGACTGC ACCGTACAAG 
AATGTGAATA TCCAGAACTT CCACATAAGC TGGAAAGATG GCCTTGGTTT CTGTGCCCTT 
ATACATCGGC ATCGCCCGGA ACTTATAGAC TACGGCAAAT TGCGCAAGGA CGATCCTCTC 
ACAAATCTAA ACACTGCTTT TGATGTTGCT GAGCGTTACC TGGACATCCC TAAGATGTTA 
GATGCTGAAG ACATTGTTGG AACAGCCCGC CCCGATGAGA AGGCCATCAT GACCTATGTT 
TCTAGCTTCT ACCACGCCTT TTCAGGAGCC CAGAAGGCAG AAACCGCTGC CAACCGCATC 
TGCAAAGTGC TGGCTGTCAA TCAGGAAAAT GAGCAGCTCA TGGAAGATTA TGAAAAGCTG 
GCCAGTGATC TGCTGGAGTG GATCCGCCGC ACAATCCCTT GGTTAGAAAA CAGGGCCCCA 
GAAAACACTA TGCAAGCCAT GCAGCAAAAG CTCGAGGATT TCCGGGACTA TCGCCGTTTG 
CATAAACCCC CTAAGGTTCA AGAGAAATGT CAACTAGAGA TCAACTTTAA CACCCTCCAA 
ACCAAGCTGA GGCTCAGCAA CCGCCCTGCA TTTATGCCTT CTGAGGGGAA GATGGTCTCG 
GACATAAATA ATGCATGGGG AGGATTGGAG CAAGCTGAGA AGGGTTATGA GGAGTGGATG 
TTGAATGAAA TCCGGAGGTT GGAAAGACTA GACCATTTAG CAGAGAAGTT CCGCCAGAAA 
GCATCTATTC ATGAAGCTTG GACTGATGGT AAAGAAGCCA TGCTGCAGAA GAAGGACTAT 
GAGACTGCAA GTCTGTCTGA TATCAAGGCT CTTCTGAAGA AACATGAGGC CTTTGAAAGT 
GACCTGGCTG CCCATCAAGA CAGAGTAGAA CAGATTGCAG CAATAGCACA GGAGCTTAAT 
GAGCTGGACT ACTATGACTC GCAAACTGTT AATGCCAGGT GCCAAAGAAT TTGTGACCAG 
TGGGATAACC TCGGAGCTCT GACACAGCAA AGAAGGGAAG CTTTAGAGAG AACTGAGAAG 
TTGCTAGAAA CCATTGATCA GCTGTACTTA GAATATGCCA AGCGAGCTGC ACCCTTCAAC 
AACTGGATGG AAGGAGCAAT GGAAGACTTA CAGGACACCT TCATTGTCCA TACCATAGAG 
GAGATTCAGG GATTAACCAC AGCGCATGAG CAATTCAAGG CAACATTACC TGAGGCTGAC 
AAAGAGCGCC AAGCAATCCT AGGAATTCAG AATGAGATAT CCAAGATCAT GCAGACGTAT 
CATGTCAATA TGTCTGGCTC AAACCCCTAC ACCAGCATTA CACCTCAAGA AATCAACAAC 
AAATGGGACC ATGTCAGACA GCTGGTCCCC CGTCGGGATC AGGCACTTAC AGAGGAACAT 
GCCCGGCAGC AGCAGAACGA GCGACTGCGG AAACAGTTTG CTGCACAGGC CAACGTCATT 
GGACCATGGA TACAAACAAA GATGCAGGAG ATCGGCCGCA TTGCTATAGA AATGCATGGC 
ACCCTGGAGG ACCAATTGAA TAATCTACGG CAGTATGAGA AAAGCATTGT AAACTACAAA 
CCAAAGATTG ACCAGTTAGA GAGTGACCAC CAACAGATTC AGGAAGCACT TATTTTTGAC 
AACAAACACA CCAACTACAC CATGGAGCAC ATTCGCGTGG GCTGGGAGCA GCTTCTAACC 
ACTATTGCCA GGACCATCAA CGAGGTAGAA AATCAGGTCC TCACAAGAGA TGCCAAAGGC 
ATTAGCCAAG AACAAATGAA TGAATTCAGG AACTCATTCA GTCATTTTGA TAGGGATCAC 
TCTGGCAGGC TTGGTCCGGA AGAGTTTAAA GCTTGCCTCA TCAGCTTGGG TTACGATATT 
GGAAACGATC CTCAGAAGAA GAGTGGTTTC TTGGAGAGTG ATGATTTCCG CGCCTGTCTT 
ATCTCCATGG GTTACAATAT GGGTGAAGCA GAATTTGCTC GAATTATGAG CATTGTTGAC 
CCCAACAGAC TTGGGCTTGT AACATTTCAA GCCTTTATTG ACTTCATGTC CCGTGAAACT 
GCAGACACTG ACACAGCAGA CCAAGTTATG GCTTCTTTCA AGATTCTTGC TGGAGATAAG 
AATTACATCA CTGCAGATGA ACTGCGCCGG GAACTGCCTC CCGATCAAGC AGAATACTGC 
ATTGCCAGAA TGGCTCCATA CCTGGGACCT GATGCTGTGC CAGGAGCTCT GGACTACATG 
TCTTTCTCAA CAGCACTATA TGGAGAAAGT GACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824128.1
CDS	146..2884
Translation	MDHYDSQNNYMQQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAERYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRAPENTMQAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNAWGGLEQAEKGYEEWMLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLQKKDYETASLSDIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSQTVNARCQRICDQWDNLGALTQQRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPEADKERQAILGIQNEISKIMQTYHVNMSGSNPYTSITPQEINNKWDHVRQLVPRRDQALTEEHARQQQNERLRKQFAAQANVIGPWIQTKMQEIGRIAIEMHGTLEDQLNNLRQYEKSIVNYKPKIDQLESDHQQIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQVLTRDAKGISQEQMNEFRNSFSHFDRDHSGRLGPEEFKACLISLGYDIGNDPQKKSGFLESDDFRACLISMGYNMGEAEFARIMSIVDPNRLGLVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITADELRRELPPDQAEYCIARMAPYLGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_012968674.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis actinin alpha 1 (actn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968674.3

ORF Insert Sequence:

ATGGATCATT ATGACTCCCA GAATAACTAT ATGCAGCAGG AAGACGATTG GGACAGGGAC 
CTCCTGTTGG ATCCAGCCTG GGAGAAGCAG CAAAGAAAGA CATTTACAGC ATGGTGCAAC 
TCACATCTCC GGAAAGCTGG GACACAGATT GAGAACATAG ATGAGGATTT CAGGGATGGC 
CTTAAACTCA TGTTATTACT TGAAGTCATT TCAGGTGAGC GTTTGGCCAA ACCTGAACGA 
GGCAAGATGC GTGTACACAA AATCTCCAAT GTCAATAAAG CCCTGGATTT TATTGCCAGT 
AAAGGTGTAA AACTGGTATC CATTGGAGCC GAAGAAATTG TGGATGGCAA TGCCAAGATG 
ACATTGGGAA TGATCTGGAC AATTATCCTG CGTTTTGCTA TCCAAGACAT TTCTGTTGAA 
GAAACATCTG CCAAAGAAGG TTTACTGCTG TGGTGTCAAA GAAAGACTGC ACCGTACAAG 
AATGTGAATA TCCAGAACTT CCACATAAGC TGGAAAGATG GCCTTGGTTT CTGTGCCCTT 
ATACATCGGC ATCGCCCGGA ACTTATAGAC TACGGCAAAT TGCGCAAGGA CGATCCTCTC 
ACAAATCTAA ACACTGCTTT TGATGTTGCT GAGCGTTACC TGGACATCCC TAAGATGTTA 
GATGCTGAAG ACATTGTTGG AACAGCCCGC CCCGATGAGA AGGCCATCAT GACCTATGTT 
TCTAGCTTCT ACCACGCCTT TTCAGGAGCC CAGAAGGCAG AAACCGCTGC CAACCGCATC 
TGCAAAGTGC TGGCTGTCAA TCAGGAAAAT GAGCAGCTCA TGGAAGATTA TGAAAAGCTG 
GCCAGTGATC TGCTGGAGTG GATCCGCCGC ACAATCCCTT GGTTAGAAAA CAGGGCCCCA 
GAAAACACTA TGCAAGCCAT GCAGCAAAAG CTCGAGGATT TCCGGGACTA TCGCCGTTTG 
CATAAACCCC CTAAGGTTCA AGAGAAATGT CAACTAGAGA TCAACTTTAA CACCCTCCAA 
ACCAAGCTGA GGCTCAGCAA CCGCCCTGCA TTTATGCCTT CTGAGGGGAA GATGGTCTCG 
GACATAAATA ATGCATGGGG AGGATTGGAG CAAGCTGAGA AGGGTTATGA GGAGTGGATG 
TTGAATGAAA TCCGGAGGTT GGAAAGACTA GACCATTTAG CAGAGAAGTT CCGCCAGAAA 
GCATCTATTC ATGAAGCTTG GACTGATGGT AAAGAAGCCA TGCTGCAGAA GAAGGACTAT 
GAGACTGCAA GTCTGTCTGA TATCAAGGCT CTTCTGAAGA AACATGAGGC CTTTGAAAGT 
GACCTGGCTG CCCATCAAGA CAGAGTAGAA CAGATTGCAG CAATAGCACA GGAGCTTAAT 
GAGCTGGACT ACTATGACTC GCAAACTGTT AATGCCAGGT GCCAAAGAAT TTGTGACCAG 
TGGGATAACC TCGGAGCTCT GACACAGCAA AGAAGGGAAG CTTTAGAGAG AACTGAGAAG 
TTGCTAGAAA CCATTGATCA GCTGTACTTA GAATATGCCA AGCGAGCTGC ACCCTTCAAC 
AACTGGATGG AAGGAGCAAT GGAAGACTTA CAGGACACCT TCATTGTCCA TACCATAGAG 
GAGATTCAGG GATTAACCAC AGCGCATGAG CAATTCAAGG CAACATTACC TGAGGCTGAC 
AAAGAGCGCC AAGCAATCCT AGGAATTCAG AATGAGATAT CCAAGATCAT GCAGACGTAT 
CATGTCAATA TGTCTGGCTC AAACCCCTAC ACCAGCATTA CACCTCAAGA AATCAACAAC 
AAATGGGACC ATGTCAGACA GCTGGTCCCC CGTCGGGATC AGGCACTTAC AGAGGAACAT 
GCCCGGCAGC AGCAGAACGA GCGACTGCGG AAACAGTTTG CTGCACAGGC CAACGTCATT 
GGACCATGGA TACAAACAAA GATGCAGGAG ATCGGCCGCA TTGCTATAGA AATGCATGGC 
ACCCTGGAGG ACCAATTGAA TAATCTACGG CAGTATGAGA AAAGCATTGT AAACTACAAA 
CCAAAGATTG ACCAGTTAGA GAGTGACCAC CAACAGATTC AGGAAGCACT TATTTTTGAC 
AACAAACACA CCAACTACAC CATGGAGCAC ATTCGCGTGG GCTGGGAGCA GCTTCTAACC 
ACTATTGCCA GGACCATCAA CGAGGTAGAA AATCAGGTCC TCACAAGAGA TGCCAAAGGC 
ATTAGCCAAG AACAAATGAA TGAATTCAGG AACTCATTCA GTCATTTTGA TAGGGATCAC 
TCTGGCAGGC TTGGTCCGGA AGAGTTTAAA GCTTGCCTCA TCAGCTTGGG TTACGATATT 
GGAAACGATC CTCAGAAGAA GAGTGGTTTC TTGGAGAGTG ATGATTTCCG CGCCTGTCTT 
ATCTCCATGG GTTACAATAT GGGTGAAGCA GAATTTGCTC GAATTATGAG CATTGTTGAC 
CCCAACAGAC TTGGGCTTGT AACATTTCAA GCCTTTATTG ACTTCATGTC CCGTGAAACT 
GCAGACACTG ACACAGCAGA CCAAGTTATG GCTTCTTTCA AGATTCTTGC TGGAGATAAG 
AATTACATCA CTGCAGATGA ACTGCGCCGG GAACTGCCTC CCGATCAAGC AGAATACTGC 
ATTGCCAGAA TGGCTCCATA CCTGGGACCT GATGCTGTGC CAGGAGCTCT GGACTACATG 
TCTTTCTCAA CAGCACTATA TGGAGAAAGT GACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

actn1 ( XM_012968675.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824129.1 Xenopus tropicalis actinin alpha 1 (actn1), transcript variant X2, mRNA.

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CloneID	OXa63814
Clone ID Related Accession (Same CDS sequence)	XM_012968675.3
Accession Version	XM_012968675.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	alpha-actinin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968675.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCATT ATGACTCCCA GAATAACTAT ATGCAGCAGG AAGACGATTG GGACAGGGAC 
CTCCTGTTGG ATCCAGCCTG GGAGAAGCAG CAAAGAAAGA CATTTACAGC ATGGTGCAAC 
TCACATCTCC GGAAAGCTGG GACACAGATT GAGAACATAG ATGAGGATTT CAGGGATGGC 
CTTAAACTCA TGTTATTACT TGAAGTCATT TCAGGTGAGC GTTTGGCCAA ACCTGAACGA 
GGCAAGATGC GTGTACACAA AATCTCCAAT GTCAATAAAG CCCTGGATTT TATTGCCAGT 
AAAGGTGTAA AACTGGTATC CATTGGAGCC GAAGAAATTG TGGATGGCAA TGCCAAGATG 
ACATTGGGAA TGATCTGGAC AATTATCCTG CGTTTTGCTA TCCAAGACAT TTCTGTTGAA 
GAAACATCTG CCAAAGAAGG TTTACTGCTG TGGTGTCAAA GAAAGACTGC ACCGTACAAG 
AATGTGAATA TCCAGAACTT CCACATAAGC TGGAAAGATG GCCTTGGTTT CTGTGCCCTT 
ATACATCGGC ATCGCCCGGA ACTTATAGAC TACGGCAAAT TGCGCAAGGA CGATCCTCTC 
ACAAATCTAA ACACTGCTTT TGATGTTGCT GAGCGTTACC TGGACATCCC TAAGATGTTA 
GATGCTGAAG ACATTGTTGG AACAGCCCGC CCCGATGAGA AGGCCATCAT GACCTATGTT 
TCTAGCTTCT ACCACGCCTT TTCAGGAGCC CAGAAGGCAG AAACCGCTGC CAACCGCATC 
TGCAAAGTGC TGGCTGTCAA TCAGGAAAAT GAGCAGCTCA TGGAAGATTA TGAAAAGCTG 
GCCAGTGATC TGCTGGAGTG GATCCGCCGC ACAATCCCTT GGTTAGAAAA CAGGGCCCCA 
GAAAACACTA TGCAAGCCAT GCAGCAAAAG CTCGAGGATT TCCGGGACTA TCGCCGTTTG 
CATAAACCCC CTAAGGTTCA AGAGAAATGT CAACTAGAGA TCAACTTTAA CACCCTCCAA 
ACCAAGCTGA GGCTCAGCAA CCGCCCTGCA TTTATGCCTT CTGAGGGGAA GATGGTCTCG 
GACATAAATA ATGCATGGGG AGGATTGGAG CAAGCTGAGA AGGGTTATGA GGAGTGGATG 
TTGAATGAAA TCCGGAGGTT GGAAAGACTA GACCATTTAG CAGAGAAGTT CCGCCAGAAA 
GCATCTATTC ATGAAGCTTG GACTGATGGT AAAGAAGCCA TGCTGCAGAA GAAGGACTAT 
GAGACTGCAA GTCTGTCTGA TATCAAGGCT CTTCTGAAGA AACATGAGGC CTTTGAAAGT 
GACCTGGCTG CCCATCAAGA CAGAGTAGAA CAGATTGCAG CAATAGCACA GGAGCTTAAT 
GAGCTGGACT ACTATGACTC GCAAACTGTT AATGCCAGGT GCCAAAGAAT TTGTGACCAG 
TGGGATAACC TCGGAGCTCT GACACAGCAA AGAAGGGAAG CTTTAGAGAG AACTGAGAAG 
TTGCTAGAAA CCATTGATCA GCTGTACTTA GAATATGCCA AGCGAGCTGC ACCCTTCAAC 
AACTGGATGG AAGGAGCAAT GGAAGACTTA CAGGACACCT TCATTGTCCA TACCATAGAG 
GAGATTCAGG GATTAACCAC AGCGCATGAG CAATTCAAGG CAACATTACC TGAGGCTGAC 
AAAGAGCGCC AAGCAATCCT AGGAATTCAG AATGAGATAT CCAAGATCAT GCAGACGTAT 
CATGTCAATA TGTCTGGCTC AAACCCCTAC ACCAGCATTA CACCTCAAGA AATCAACAAC 
AAATGGGACC ATGTCAGACA GCTGGTCCCC CGTCGGGATC AGGCACTTAC AGAGGAACAT 
GCCCGGCAGC AGCAGAACGA GCGACTGCGG AAACAGTTTG CTGCACAGGC CAACGTCATT 
GGACCATGGA TACAAACAAA GATGCAGGAG ATCGGCCGCA TTGCTATAGA AATGCATGGC 
ACCCTGGAGG ACCAATTGAA TAATCTACGG CAGTATGAGA AAAGCATTGT AAACTACAAA 
CCAAAGATTG ACCAGTTAGA GAGTGACCAC CAACAGATTC AGGAAGCACT TATTTTTGAC 
AACAAACACA CCAACTACAC CATGGAGCAC ATTCGCGTGG GCTGGGAGCA GCTTCTAACC 
ACTATTGCCA GGACCATCAA CGAGGTAGAA AATCAGGTCC TCACAAGAGA TGCCAAAGGC 
ATTAGCCAAG AACAAATGAA TGAATTCAGG AACTCATTCA GTCATTTTGA TAGGGATCAC 
TCTGGCAGGC TTGGTCCGGA AGAGTTTAAA GCTTGCCTCA TCAGCTTGGG TTACGATATT 
GGAAACGATC CTCAGGGTGA AGCAGAATTT GCTCGAATTA TGAGCATTGT TGACCCCAAC 
AGACTTGGGC TTGTAACATT TCAAGCCTTT ATTGACTTCA TGTCCCGTGA AACTGCAGAC 
ACTGACACAG CAGACCAAGT TATGGCTTCT TTCAAGATTC TTGCTGGAGA TAAGAATTAC 
ATCACTGCAG ATGAACTGCG CCGGGAACTG CCTCCCGATC AAGCAGAATA CTGCATTGCC 
AGAATGGCTC CATACCTGGG ACCTGATGCT GTGCCAGGAG CTCTGGACTA CATGTCTTTC 
TCAACAGCAC TATATGGAGA AAGTGACCTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824129.1
CDS	146..2818
Translation	MDHYDSQNNYMQQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAERYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRAPENTMQAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNAWGGLEQAEKGYEEWMLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLQKKDYETASLSDIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSQTVNARCQRICDQWDNLGALTQQRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPEADKERQAILGIQNEISKIMQTYHVNMSGSNPYTSITPQEINNKWDHVRQLVPRRDQALTEEHARQQQNERLRKQFAAQANVIGPWIQTKMQEIGRIAIEMHGTLEDQLNNLRQYEKSIVNYKPKIDQLESDHQQIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQVLTRDAKGISQEQMNEFRNSFSHFDRDHSGRLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGLVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITADELRRELPPDQAEYCIARMAPYLGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_012968675.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis actinin alpha 1 (actn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968675.3

ORF Insert Sequence:

ATGGATCATT ATGACTCCCA GAATAACTAT ATGCAGCAGG AAGACGATTG GGACAGGGAC 
CTCCTGTTGG ATCCAGCCTG GGAGAAGCAG CAAAGAAAGA CATTTACAGC ATGGTGCAAC 
TCACATCTCC GGAAAGCTGG GACACAGATT GAGAACATAG ATGAGGATTT CAGGGATGGC 
CTTAAACTCA TGTTATTACT TGAAGTCATT TCAGGTGAGC GTTTGGCCAA ACCTGAACGA 
GGCAAGATGC GTGTACACAA AATCTCCAAT GTCAATAAAG CCCTGGATTT TATTGCCAGT 
AAAGGTGTAA AACTGGTATC CATTGGAGCC GAAGAAATTG TGGATGGCAA TGCCAAGATG 
ACATTGGGAA TGATCTGGAC AATTATCCTG CGTTTTGCTA TCCAAGACAT TTCTGTTGAA 
GAAACATCTG CCAAAGAAGG TTTACTGCTG TGGTGTCAAA GAAAGACTGC ACCGTACAAG 
AATGTGAATA TCCAGAACTT CCACATAAGC TGGAAAGATG GCCTTGGTTT CTGTGCCCTT 
ATACATCGGC ATCGCCCGGA ACTTATAGAC TACGGCAAAT TGCGCAAGGA CGATCCTCTC 
ACAAATCTAA ACACTGCTTT TGATGTTGCT GAGCGTTACC TGGACATCCC TAAGATGTTA 
GATGCTGAAG ACATTGTTGG AACAGCCCGC CCCGATGAGA AGGCCATCAT GACCTATGTT 
TCTAGCTTCT ACCACGCCTT TTCAGGAGCC CAGAAGGCAG AAACCGCTGC CAACCGCATC 
TGCAAAGTGC TGGCTGTCAA TCAGGAAAAT GAGCAGCTCA TGGAAGATTA TGAAAAGCTG 
GCCAGTGATC TGCTGGAGTG GATCCGCCGC ACAATCCCTT GGTTAGAAAA CAGGGCCCCA 
GAAAACACTA TGCAAGCCAT GCAGCAAAAG CTCGAGGATT TCCGGGACTA TCGCCGTTTG 
CATAAACCCC CTAAGGTTCA AGAGAAATGT CAACTAGAGA TCAACTTTAA CACCCTCCAA 
ACCAAGCTGA GGCTCAGCAA CCGCCCTGCA TTTATGCCTT CTGAGGGGAA GATGGTCTCG 
GACATAAATA ATGCATGGGG AGGATTGGAG CAAGCTGAGA AGGGTTATGA GGAGTGGATG 
TTGAATGAAA TCCGGAGGTT GGAAAGACTA GACCATTTAG CAGAGAAGTT CCGCCAGAAA 
GCATCTATTC ATGAAGCTTG GACTGATGGT AAAGAAGCCA TGCTGCAGAA GAAGGACTAT 
GAGACTGCAA GTCTGTCTGA TATCAAGGCT CTTCTGAAGA AACATGAGGC CTTTGAAAGT 
GACCTGGCTG CCCATCAAGA CAGAGTAGAA CAGATTGCAG CAATAGCACA GGAGCTTAAT 
GAGCTGGACT ACTATGACTC GCAAACTGTT AATGCCAGGT GCCAAAGAAT TTGTGACCAG 
TGGGATAACC TCGGAGCTCT GACACAGCAA AGAAGGGAAG CTTTAGAGAG AACTGAGAAG 
TTGCTAGAAA CCATTGATCA GCTGTACTTA GAATATGCCA AGCGAGCTGC ACCCTTCAAC 
AACTGGATGG AAGGAGCAAT GGAAGACTTA CAGGACACCT TCATTGTCCA TACCATAGAG 
GAGATTCAGG GATTAACCAC AGCGCATGAG CAATTCAAGG CAACATTACC TGAGGCTGAC 
AAAGAGCGCC AAGCAATCCT AGGAATTCAG AATGAGATAT CCAAGATCAT GCAGACGTAT 
CATGTCAATA TGTCTGGCTC AAACCCCTAC ACCAGCATTA CACCTCAAGA AATCAACAAC 
AAATGGGACC ATGTCAGACA GCTGGTCCCC CGTCGGGATC AGGCACTTAC AGAGGAACAT 
GCCCGGCAGC AGCAGAACGA GCGACTGCGG AAACAGTTTG CTGCACAGGC CAACGTCATT 
GGACCATGGA TACAAACAAA GATGCAGGAG ATCGGCCGCA TTGCTATAGA AATGCATGGC 
ACCCTGGAGG ACCAATTGAA TAATCTACGG CAGTATGAGA AAAGCATTGT AAACTACAAA 
CCAAAGATTG ACCAGTTAGA GAGTGACCAC CAACAGATTC AGGAAGCACT TATTTTTGAC 
AACAAACACA CCAACTACAC CATGGAGCAC ATTCGCGTGG GCTGGGAGCA GCTTCTAACC 
ACTATTGCCA GGACCATCAA CGAGGTAGAA AATCAGGTCC TCACAAGAGA TGCCAAAGGC 
ATTAGCCAAG AACAAATGAA TGAATTCAGG AACTCATTCA GTCATTTTGA TAGGGATCAC 
TCTGGCAGGC TTGGTCCGGA AGAGTTTAAA GCTTGCCTCA TCAGCTTGGG TTACGATATT 
GGAAACGATC CTCAGGGTGA AGCAGAATTT GCTCGAATTA TGAGCATTGT TGACCCCAAC 
AGACTTGGGC TTGTAACATT TCAAGCCTTT ATTGACTTCA TGTCCCGTGA AACTGCAGAC 
ACTGACACAG CAGACCAAGT TATGGCTTCT TTCAAGATTC TTGCTGGAGA TAAGAATTAC 
ATCACTGCAG ATGAACTGCG CCGGGAACTG CCTCCCGATC AAGCAGAATA CTGCATTGCC 
AGAATGGCTC CATACCTGGG ACCTGATGCT GTGCCAGGAG CTCTGGACTA CATGTCTTTC 
TCAACAGCAC TATATGGAGA AAGTGACCTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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actn1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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