tdrd12 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	tdrd12
Entrez Gene ID	780068
Full Name	tudor domain containing 12
Synonyms	ecat8
General protein information	Preferred Names tudor domain containing 12 Names putative ATP-dependent RNA helicase TDRD12
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018093101.1	XP_017948590.1	putative ATP-dependent RNA helicase TDRD12 isoform X1
NM_001079144.1	NP_001072612.1	putative ATP-dependent RNA helicase TDRD12
XM_031900806.1	XP_031756666.1	putative ATP-dependent RNA helicase TDRD12
XM_012961356.2	XP_012816810.1	putative ATP-dependent RNA helicase TDRD12 isoform X1
XM_018093101.2	XP_017948590.2	putative ATP-dependent RNA helicase TDRD12
XM_012961356.3	XP_012816810.2	putative ATP-dependent RNA helicase TDRD12

Pathways from BioSystems

Homologies

	TDRD12	XP_001087212.2
Bos taurus (cattle)	TDRD12	XP_002695045.2
Danio rerio (zebrafish)	LOC100334979	XP_005163266.1
Xenopus tropicalis (tropical clawed frog)	tdrd12	NP_001072612.1
Homo sapiens (human)	LOC101929327	XP_006723595.1
Mus musculus (house mouse)	Tdrd12	NP_082310.1
Rattus norvegicus (Norway rat)	Tdrd12	XP_006228963.1
Canis lupus familiaris (dog)	TDRD12	XP_005616833.1
Gallus gallus (chicken)	TDRD12	XP_001232125.3

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tdrd12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tdrd12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa03180	NM_001079144.1 Latest version!	Xenopus tropicalis tudor domain containing 12 (tdrd12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa56928	XM_018093101.2 Latest version!	Xenopus tropicalis tudor domain containing 12 (tdrd12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa56928	XM_012961356.3 Latest version!	Xenopus tropicalis tudor domain containing 12 (tdrd12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa56928	XM_031900806.1 Latest version!	Xenopus tropicalis tudor domain containing 12 (tdrd12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa29419	XM_018093101.1 Latest version!	Xenopus tropicalis tudor domain containing 12 (tdrd12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa29419	XM_012961356.2 Latest version!	Xenopus tropicalis tudor domain containing 12 (tdrd12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tdrd12 ( NM_001079144.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001072612.1 Xenopus tropicalis tudor domain containing 12 (tdrd12), mRNA.

Price & Availability↑

CloneID	OXa03180
Clone ID Related Accession (Same CDS sequence)	NM_001079144.1
Accession Version	NM_001079144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-27
Organism	Xenopus tropicalis(tropical clawed frog)
Product	putative ATP-dependent RNA helicase TDRD12
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC122994.1. ##Evidence-Data-START## Transcript exon combination :: BC122994.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1025683, SAMEA1025684 [ECO:0000350] ##Evidence-Data-END##

ATGTATGAAG TGGCAGTATT GCAGATTCAG GACCCCAGCT GCTTTTGGTG TGAAATATTA 
AAAAGCAATG GCCAAGATAT GAAGGAGAAA GTGGAATATG AGCAGCTGTA TAATGATCTT 
AATTGTCTTT ATGGAAAGGT CTACAGAAAT GTAGAGGAAA TAAAGCCAGC ATTTGTATCA 
GTTGGAGAGT TTTGCATGGT TTTTTGCGAA GACCTTAATA GTTGGTGCCG AGCTATCCTA 
AATGCACCTG CATGTAGTGC AGAAGATAAC CTTGTGGAGT GCTTCCTAGT GGATCATGCC 
ATTTACTGTC CTGTCAAAAA GAAAAACATT CGCCTTCCTG TGGAAGCTTG TCATCGTCTT 
CCTTTTCGTG CAAAAAAGTT TAAACTTCAC CAAATTCAGC CAGTATCTTT GTGTGTTGAT 
CTTTGTGGAG ATAAAGCAGA ACTTGGAGTT GCTGACAAAT GGGACGCTGC TGCTATTGAG 
TATTTTAAAC ATCTTCTAAA GGAGTCCGTT CATGCTGAAG CAAAATTATT CTCTATAGAA 
AACAATATTT TGAGTGTATA TCTTTACATC GTTACTACAG AGGCTACGGT GTGTGTTAAT 
GATGAACTGG TGGCAAAAAG CTTTGCTTGT TTCTGTTCTG ATGTACAATC TTGTGAAAAA 
ATCATTCCTA CAAGTCTACA AAATATGCAA CCTTCCAAGG AAGTTGATAA TCCTGCACAG 
ATGTTTTGGC CTGAGATCTA TAAAACAAAA AAAATACCAT TTTATGAAGG TTTTCCAATA 
AATGAAAAGG ATGAAATGGA GCCCAGAAAT CACTGTGAAA AGAAACCTGA AGAAAAAGAG 
ATGTGGGAAG AAAACCATGA CTTTGACAAA CTCCTGCAGA TTATCAATCC AGAACCCATA 
AAACTTTCTG AAAATGATTA CATACAGAAA CCAAGAACCA GTGCACCACA ATACCCAATT 
TTTGTGCGCA AAACTATCGA ACAGTGTTGT ACACTTAAAT TTACATCTCT GACGTCTGAT 
CTTAAAAAGG AGCTTGTGAG AAATCGTTAC TGTGGACCAA GTTTCACTGA ATCCTACAGC 
TGGCCTTCTG TAGCACAGGG ATTTAATACC ATTGTAATAT CACCTGATGG CATGAAACCC 
ATGAACTATA TTCCTCCTCT CCTCACATTT TTACACCTTG CTTCAGCAGT GTATACATTA 
CTGCCATCTA GAAATGGGCC TCTTGCAGCC ATTCTTTGCT CTGGATGGAA GAAAGCTCAT 
TTTGTACATG AACTACTTTT AAAGTACAGT AAACACACCA GACCCCTTAA TCCGATGTTG 
ATATTAGTTG GGCTAAAAGA GGAAGAATGC GAAAATCTTA ACATTCGAAA AGGATGTGAG 
GTGATTGTGA CCACACCACC TAGTCTTCTT AGATATCTGA AATGTAAGGG ATTGCTGCTG 
TTAAGGCTTT GTCATCTTGT CTTAGATGAA GTAGACATCC TTTTTTTTAA AATCAGGATC 
ACAGGTAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072612.1
CDS	156..1667
Translation	MYEVAVLQIQDPSCFWCEILKSNGQDMKEKVEYEQLYNDLNCLYGKVYRNVEEIKPAFVSVGEFCMVFCEDLNSWCRAILNAPACSAEDNLVECFLVDHAIYCPVKKKNIRLPVEACHRLPFRAKKFKLHQIQPVSLCVDLCGDKAELGVADKWDAAAIEYFKHLLKESVHAEAKLFSIENNILSVYLYIVTTEATVCVNDELVAKSFACFCSDVQSCEKIIPTSLQNMQPSKEVDNPAQMFWPEIYKTKKIPFYEGFPINEKDEMEPRNHCEKKPEEKEMWEENHDFDKLLQIINPEPIKLSENDYIQKPRTSAPQYPIFVRKTIEQCCTLKFTSLTSDLKKELVRNRYCGPSFTESYSWPSVAQGFNTIVISPDGMKPMNYIPPLLTFLHLASAVYTLLPSRNGPLAAILCSGWKKAHFVHELLLKYSKHTRPLNPMLILVGLKEEECENLNIRKGCEVIVTTPPSLLRYLKCKGLLLLRLCHLVLDEVDILFFKIRITGN

Target ORF information:

RefSeq Version	NM_001079144.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tudor domain containing 12 (tdrd12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079144.1

ORF Insert Sequence:

ATGTATGAAG TGGCAGTATT GCAGATTCAG GACCCCAGCT GCTTTTGGTG TGAAATATTA 
AAAAGCAATG GCCAAGATAT GAAGGAGAAA GTGGAATATG AGCAGCTGTA TAATGATCTT 
AATTGTCTTT ATGGAAAGGT CTACAGAAAT GTAGAGGAAA TAAAGCCAGC ATTTGTATCA 
GTTGGAGAGT TTTGCATGGT TTTTTGCGAA GACCTTAATA GTTGGTGCCG AGCTATCCTA 
AATGCACCTG CATGTAGTGC AGAAGATAAC CTTGTGGAGT GCTTCCTAGT GGATCATGCC 
ATTTACTGTC CTGTCAAAAA GAAAAACATT CGCCTTCCTG TGGAAGCTTG TCATCGTCTT 
CCTTTTCGTG CAAAAAAGTT TAAACTTCAC CAAATTCAGC CAGTATCTTT GTGTGTTGAT 
CTTTGTGGAG ATAAAGCAGA ACTTGGAGTT GCTGACAAAT GGGACGCTGC TGCTATTGAG 
TATTTTAAAC ATCTTCTAAA GGAGTCCGTT CATGCTGAAG CAAAATTATT CTCTATAGAA 
AACAATATTT TGAGTGTATA TCTTTACATC GTTACTACAG AGGCTACGGT GTGTGTTAAT 
GATGAACTGG TGGCAAAAAG CTTTGCTTGT TTCTGTTCTG ATGTACAATC TTGTGAAAAA 
ATCATTCCTA CAAGTCTACA AAATATGCAA CCTTCCAAGG AAGTTGATAA TCCTGCACAG 
ATGTTTTGGC CTGAGATCTA TAAAACAAAA AAAATACCAT TTTATGAAGG TTTTCCAATA 
AATGAAAAGG ATGAAATGGA GCCCAGAAAT CACTGTGAAA AGAAACCTGA AGAAAAAGAG 
ATGTGGGAAG AAAACCATGA CTTTGACAAA CTCCTGCAGA TTATCAATCC AGAACCCATA 
AAACTTTCTG AAAATGATTA CATACAGAAA CCAAGAACCA GTGCACCACA ATACCCAATT 
TTTGTGCGCA AAACTATCGA ACAGTGTTGT ACACTTAAAT TTACATCTCT GACGTCTGAT 
CTTAAAAAGG AGCTTGTGAG AAATCGTTAC TGTGGACCAA GTTTCACTGA ATCCTACAGC 
TGGCCTTCTG TAGCACAGGG ATTTAATACC ATTGTAATAT CACCTGATGG CATGAAACCC 
ATGAACTATA TTCCTCCTCT CCTCACATTT TTACACCTTG CTTCAGCAGT GTATACATTA 
CTGCCATCTA GAAATGGGCC TCTTGCAGCC ATTCTTTGCT CTGGATGGAA GAAAGCTCAT 
TTTGTACATG AACTACTTTT AAAGTACAGT AAACACACCA GACCCCTTAA TCCGATGTTG 
ATATTAGTTG GGCTAAAAGA GGAAGAATGC GAAAATCTTA ACATTCGAAA AGGATGTGAG 
GTGATTGTGA CCACACCACC TAGTCTTCTT AGATATCTGA AATGTAAGGG ATTGCTGCTG 
TTAAGGCTTT GTCATCTTGT CTTAGATGAA GTAGACATCC TTTTTTTTAA AATCAGGATC 
ACAGGTAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tdrd12 ( XM_031900806.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756666.1 Xenopus tropicalis tudor domain containing 12 (tdrd12), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa56928
Clone ID Related Accession (Same CDS sequence)	XM_031900806.1 , XM_018093101.2 , XM_012961356.3
Accession Version	XM_031900806.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	putative ATP-dependent RNA helicase TDRD12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAAG TGGCAGTATT GCAGATTCAG GACCCCAGCT GCTTTTGGTG TGAAATATTA 
AAAAGCAATG GCCAAGATAT GAAGGAGAAA GTGGAATATG AGCAGCTGTA TAATGATCTT 
AATTGTCTTT ATGGAAAGGT CTACAGAAAT GTAGAGGAAA TAAAGCCAGC ATTTGTATCA 
GTTGGAGAGT TTTGCATGGT TTTTTGCGAA GACCTTAATA GTTGGTGCCG AGCTATCCTA 
AATGCACCTG CATGTAGTGC AGAAGATAAC CTTGTGGAGT GCTTCCTAGT GGATCATGCC 
ATTTACTGTC CTGTCAAAAA GAAAAACATT CGCCTTCCTG TGGAAGCTTG TCATCGTCTT 
CCTTTTCGTG CAAAAAAGTT TAAACTTCAC CAAATTCAGC CAGTATCTTT GTGTGTTGAT 
CTTTGTGGAG ATAAAGCAGA ACTTGGAGTT GCTGACAAAT GGGACGCTGC TGCTATTGAG 
TATTTTAAAC ATCTTCTAAA GGAGTCCGTT CATGCTGAAG CAAAATTATT CTCTATAGAA 
AACAATATTT TGAGTGTATA TCTTTACATC GTTACTACAG AGGCTACGGT GTGTGTTAAT 
GATGAACTGG TGGCAAAAAG CTTTGCTTGT TTCTGTTCTG ATGTACAATC TTGTGAAAAA 
ATCATTCCTA CAAGTCTACA AAATATGCAA CCTTCCAAGG AAGTTGATAA TCCTGCACAG 
ATGTTTTGGC CTGAGATCTA TAAAACAAAA AAAATACCAT TTTATGAAGG TTTTCCAATA 
AATGAAAAGG ATGAAATGGA GCCCAGAAAT CACTGTGAAA AGAAACCTGA AGAAAAAGAG 
ATGTGGGAAG AAAACCATGA CTTTGACAAA CTCCTGCAGA TTATCAATCC AGAACCCATA 
AAACTTTCTG AAAATGATTA CATACAGAAA CCAAGAACCA GTGCACCACA ATACCCAATT 
TTTGTGCGCA AAACTATCGA ACAGTGTTGT ACACTTAAAT TTACATCTCT GACGTCTGAT 
CTTAAAAAGG AGCTTGTGAG AAATCGTTAC TGTGGACCAA GTTTCACTGA ATCCTACAGC 
TGGCCTTCTG TAGCACAGGG ATTTAATACC ATTGTAATAT CACCTGATGG CATGAAACCC 
ATGAACTATA TTCCTCCTCT CCTCACATTT TTACACCTTG CTTCAGCAGT GTATACATTA 
CTGCCATCTA GAAATGGGCC TCTTGCAGCC ATTCTTTGCT CTGGATGGAA GAAAGCTCAT 
TTTGTACATG AACTACTTTT AAAGTACAGT AAACACACCA GACCCCTTAA TCCGATGTTG 
ATATTAGTTG GGCTAAAAGA GGAAGAATGC GAAAATCTTA ACATTCGAAA AGGATGTGAG 
GTGATTGTGA CCACACCACC TAGTCTTCTT AGATATCTGA AATGTAAGGG ATTGCTGCTG 
TTAAGGCTTT GTCATCTTGT CTTAGATGAA GTAGACATCC TTTTTTTAAA ATCAGGATCA 
CAGGTAATTG AAATTCTGGA ACTTTTTAAG AAATCAGTTG CAGCAGAAAG CCGAGAATGT 
GCACCTCAGC AGATTGTAGC TACTGGAACA TTTTGGCACA AGGAAATGAA TTCTTTACTG 
CAATATACAA CAGAACCTCA GGTCATAATC ACCAGAATGG AACAGGCTGC AGTGTATGGC 
AATGTCCAGC AGGTGATACA ACTTTGTTTA GACTGTGAAA AAATATCGGT ACTGCTACGG 
AATTTGGATT TCACACCAGA CCATGCTCAA AAGGTCTTGA TATTTGTTGA ATCTGATGAA 
GAGGCTGAGC TGGTTCACAA GGCTGTCCAA AACCATTCCA TTTTCAGTTT AGTTGTAAAC 
AGGAAGCAAG TGCAAAGTTT TAGCAGTGTG TTACAACAAT GGAAAAATAT GTTAAGCCGT 
GGAACACACA TTGTTTTAGT TGTTACAAAT GAATATTTAC CTCTTCTTGA AGTGACAGAT 
GCCACATGTA TAATACACTT CAGTTTTCCA GAAAATGAAA ATGTTTTTGG TCTGCGCCTC 
TATTCCTTAC TGGACTACAT ACACAGTAAA ACAGAGCGGG TAATTTCTTT AGAAGACAGT 
TTCCCAGTAG CCAGATCTGT TTTGCTTATG ACAGAAAAAC ATATACACCA TTCAACTGAT 
ATACTGCGTT ACTTACAACA CACAGAGGCC ACAATTCCGC CAGAGTTAAG TGATCTTACG 
CAAGGAATTT TACAGGCAAA TGAGAATAGG AAGTTTGATA AAGATATTTG TCAGCATATC 
AAAACTTTTG GTGTTTGCAG GTTAAATCTA TTTACATGCC CTGATCGCCA TAACCTCCTT 
CCATCTACAG ACCATCAGTA TTCCATGACA ACAACCAACC AGTACATATC TGTTGTGCCA 
GTACATGTTT TGGATCCAGT TCACTTTTTT GGAAGAATAG TAACTAAAGA GGATGTGTAT 
ACTCATCTAC TTGAAGATCT GAATAAATTT TACCAAATAC CAGGCAATCA AATATTAGTC 
CAGGAAGTAA ACGTTTCACA ATTATATGCT GTACAAGAGG GATCTGTTTA TCACAGGGTT 
AAAGTATTTT CTGCCAAAAA GGAGAATGGA GTTTTGAATT TTAGTTTGCA ATTCATTGAT 
GATGGAAGAA CTGATAAAGT TAGAGATGCC CAACTGTTGC AGTTACCTTC TCAATTTGAA 
AAAATTCCAC CACAGTCAGC TGAGTTTATT GTATGCAGGG TTCAACCAAT TGATAATGAG 
GCTGAATGGG ATCCCATTGT AACAAGGACC ATTAGTAAAG CCATAAAAGG AAAACTTCAT 
ACTGCAAAAG TTGTACTCCA TATTGGGAAT ACATATTGGT TGGATCCAAT GGTGCAAAGA 
AGCAAACTGT CTGGCTTTGC AGCTTGTATA AATGAATTAA ATGTGCGCCA TGAGATCTTG 
TCTACTGGGA TGGGAGTTGA TAATCCTCAG CACATAAGTC ATCTTGAGGA ATTATTGAAA 
AAAACTGGAG TAGCAACTGT GCAGCAAGAC TCTACTGATA AGGTATCAGA TGCAGACTTG 
TCAAGCAATA ATATGATCGC CAATTGCTGT GTTTCTTCTC TTTCCAGTTT GGATGCAAAC 
TGTGGTGCAA GGAATGTGGA CCTACAAAAT AAACTGGAGC GTAATCCACT AGAAAGTAAA 
AGCTTGCAAC CAGAAGTGAA ATGGTTTCAG AAAGATAAAG CAGTAATCCT GACGATAAAG 
TTACATGATG TTACAGATCA TGCCTGTATG TTTTATGCTT CCAGAGTTGT GTTTAGTTGT 
TATGCTGGAG GAAAGCACTA TGTGGCAGAT CTGGAACTTT GTGAGGAGAT ACTAAACGAA 
GAATGTGAGT GCTGCATTAA GAATGGAGAA CCTGTAATAA CATTATTGAA GGCTCAAAAC 
AGACCTTGGA AAAGCCTTCT GAAACAAAAG CATCCAAATG TGAGTCTTGA TTTTGACAAA 
TGGGAAGATT CTGAGGACAG TGATGGTTTT CCTTTTGTCT CCGGAAAGCC CAAGTGTCTT 
TACAAAGTTG TAAGTGAAGA AGTTGAATCT TCGGAATACA GTGAATCTGA CACTGAATCT 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756666.1
CDS	80..3685
Translation	MYEVAVLQIQDPSCFWCEILKSNGQDMKEKVEYEQLYNDLNCLYGKVYRNVEEIKPAFVSVGEFCMVFCEDLNSWCRAILNAPACSAEDNLVECFLVDHAIYCPVKKKNIRLPVEACHRLPFRAKKFKLHQIQPVSLCVDLCGDKAELGVADKWDAAAIEYFKHLLKESVHAEAKLFSIENNILSVYLYIVTTEATVCVNDELVAKSFACFCSDVQSCEKIIPTSLQNMQPSKEVDNPAQMFWPEIYKTKKIPFYEGFPINEKDEMEPRNHCEKKPEEKEMWEENHDFDKLLQIINPEPIKLSENDYIQKPRTSAPQYPIFVRKTIEQCCTLKFTSLTSDLKKELVRNRYCGPSFTESYSWPSVAQGFNTIVISPDGMKPMNYIPPLLTFLHLASAVYTLLPSRNGPLAAILCSGWKKAHFVHELLLKYSKHTRPLNPMLILVGLKEEECENLNIRKGCEVIVTTPPSLLRYLKCKGLLLLRLCHLVLDEVDILFLKSGSQVIEILELFKKSVAAESRECAPQQIVATGTFWHKEMNSLLQYTTEPQVIITRMEQAAVYGNVQQVIQLCLDCEKISVLLRNLDFTPDHAQKVLIFVESDEEAELVHKAVQNHSIFSLVVNRKQVQSFSSVLQQWKNMLSRGTHIVLVVTNEYLPLLEVTDATCIIHFSFPENENVFGLRLYSLLDYIHSKTERVISLEDSFPVARSVLLMTEKHIHHSTDILRYLQHTEATIPPELSDLTQGILQANENRKFDKDICQHIKTFGVCRLNLFTCPDRHNLLPSTDHQYSMTTTNQYISVVPVHVLDPVHFFGRIVTKEDVYTHLLEDLNKFYQIPGNQILVQEVNVSQLYAVQEGSVYHRVKVFSAKKENGVLNFSLQFIDDGRTDKVRDAQLLQLPSQFEKIPPQSAEFIVCRVQPIDNEAEWDPIVTRTISKAIKGKLHTAKVVLHIGNTYWLDPMVQRSKLSGFAACINELNVRHEILSTGMGVDNPQHISHLEELLKKTGVATVQQDSTDKVSDADLSSNNMIANCCVSSLSSLDANCGARNVDLQNKLERNPLESKSLQPEVKWFQKDKAVILTIKLHDVTDHACMFYASRVVFSCYAGGKHYVADLELCEEILNEECECCIKNGEPVITLLKAQNRPWKSLLKQKHPNVSLDFDKWEDSEDSDGFPFVSGKPKCLYKVVSEEVESSEYSESDTESD

Target ORF information:

RefSeq Version	XM_031900806.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tudor domain containing 12 (tdrd12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900806.1

ORF Insert Sequence:

ATGTATGAAG TGGCAGTATT GCAGATTCAG GACCCCAGCT GCTTTTGGTG TGAAATATTA 
AAAAGCAATG GCCAAGATAT GAAGGAGAAA GTGGAATATG AGCAGCTGTA TAATGATCTT 
AATTGTCTTT ATGGAAAGGT CTACAGAAAT GTAGAGGAAA TAAAGCCAGC ATTTGTATCA 
GTTGGAGAGT TTTGCATGGT TTTTTGCGAA GACCTTAATA GTTGGTGCCG AGCTATCCTA 
AATGCACCTG CATGTAGTGC AGAAGATAAC CTTGTGGAGT GCTTCCTAGT GGATCATGCC 
ATTTACTGTC CTGTCAAAAA GAAAAACATT CGCCTTCCTG TGGAAGCTTG TCATCGTCTT 
CCTTTTCGTG CAAAAAAGTT TAAACTTCAC CAAATTCAGC CAGTATCTTT GTGTGTTGAT 
CTTTGTGGAG ATAAAGCAGA ACTTGGAGTT GCTGACAAAT GGGACGCTGC TGCTATTGAG 
TATTTTAAAC ATCTTCTAAA GGAGTCCGTT CATGCTGAAG CAAAATTATT CTCTATAGAA 
AACAATATTT TGAGTGTATA TCTTTACATC GTTACTACAG AGGCTACGGT GTGTGTTAAT 
GATGAACTGG TGGCAAAAAG CTTTGCTTGT TTCTGTTCTG ATGTACAATC TTGTGAAAAA 
ATCATTCCTA CAAGTCTACA AAATATGCAA CCTTCCAAGG AAGTTGATAA TCCTGCACAG 
ATGTTTTGGC CTGAGATCTA TAAAACAAAA AAAATACCAT TTTATGAAGG TTTTCCAATA 
AATGAAAAGG ATGAAATGGA GCCCAGAAAT CACTGTGAAA AGAAACCTGA AGAAAAAGAG 
ATGTGGGAAG AAAACCATGA CTTTGACAAA CTCCTGCAGA TTATCAATCC AGAACCCATA 
AAACTTTCTG AAAATGATTA CATACAGAAA CCAAGAACCA GTGCACCACA ATACCCAATT 
TTTGTGCGCA AAACTATCGA ACAGTGTTGT ACACTTAAAT TTACATCTCT GACGTCTGAT 
CTTAAAAAGG AGCTTGTGAG AAATCGTTAC TGTGGACCAA GTTTCACTGA ATCCTACAGC 
TGGCCTTCTG TAGCACAGGG ATTTAATACC ATTGTAATAT CACCTGATGG CATGAAACCC 
ATGAACTATA TTCCTCCTCT CCTCACATTT TTACACCTTG CTTCAGCAGT GTATACATTA 
CTGCCATCTA GAAATGGGCC TCTTGCAGCC ATTCTTTGCT CTGGATGGAA GAAAGCTCAT 
TTTGTACATG AACTACTTTT AAAGTACAGT AAACACACCA GACCCCTTAA TCCGATGTTG 
ATATTAGTTG GGCTAAAAGA GGAAGAATGC GAAAATCTTA ACATTCGAAA AGGATGTGAG 
GTGATTGTGA CCACACCACC TAGTCTTCTT AGATATCTGA AATGTAAGGG ATTGCTGCTG 
TTAAGGCTTT GTCATCTTGT CTTAGATGAA GTAGACATCC TTTTTTTAAA ATCAGGATCA 
CAGGTAATTG AAATTCTGGA ACTTTTTAAG AAATCAGTTG CAGCAGAAAG CCGAGAATGT 
GCACCTCAGC AGATTGTAGC TACTGGAACA TTTTGGCACA AGGAAATGAA TTCTTTACTG 
CAATATACAA CAGAACCTCA GGTCATAATC ACCAGAATGG AACAGGCTGC AGTGTATGGC 
AATGTCCAGC AGGTGATACA ACTTTGTTTA GACTGTGAAA AAATATCGGT ACTGCTACGG 
AATTTGGATT TCACACCAGA CCATGCTCAA AAGGTCTTGA TATTTGTTGA ATCTGATGAA 
GAGGCTGAGC TGGTTCACAA GGCTGTCCAA AACCATTCCA TTTTCAGTTT AGTTGTAAAC 
AGGAAGCAAG TGCAAAGTTT TAGCAGTGTG TTACAACAAT GGAAAAATAT GTTAAGCCGT 
GGAACACACA TTGTTTTAGT TGTTACAAAT GAATATTTAC CTCTTCTTGA AGTGACAGAT 
GCCACATGTA TAATACACTT CAGTTTTCCA GAAAATGAAA ATGTTTTTGG TCTGCGCCTC 
TATTCCTTAC TGGACTACAT ACACAGTAAA ACAGAGCGGG TAATTTCTTT AGAAGACAGT 
TTCCCAGTAG CCAGATCTGT TTTGCTTATG ACAGAAAAAC ATATACACCA TTCAACTGAT 
ATACTGCGTT ACTTACAACA CACAGAGGCC ACAATTCCGC CAGAGTTAAG TGATCTTACG 
CAAGGAATTT TACAGGCAAA TGAGAATAGG AAGTTTGATA AAGATATTTG TCAGCATATC 
AAAACTTTTG GTGTTTGCAG GTTAAATCTA TTTACATGCC CTGATCGCCA TAACCTCCTT 
CCATCTACAG ACCATCAGTA TTCCATGACA ACAACCAACC AGTACATATC TGTTGTGCCA 
GTACATGTTT TGGATCCAGT TCACTTTTTT GGAAGAATAG TAACTAAAGA GGATGTGTAT 
ACTCATCTAC TTGAAGATCT GAATAAATTT TACCAAATAC CAGGCAATCA AATATTAGTC 
CAGGAAGTAA ACGTTTCACA ATTATATGCT GTACAAGAGG GATCTGTTTA TCACAGGGTT 
AAAGTATTTT CTGCCAAAAA GGAGAATGGA GTTTTGAATT TTAGTTTGCA ATTCATTGAT 
GATGGAAGAA CTGATAAAGT TAGAGATGCC CAACTGTTGC AGTTACCTTC TCAATTTGAA 
AAAATTCCAC CACAGTCAGC TGAGTTTATT GTATGCAGGG TTCAACCAAT TGATAATGAG 
GCTGAATGGG ATCCCATTGT AACAAGGACC ATTAGTAAAG CCATAAAAGG AAAACTTCAT 
ACTGCAAAAG TTGTACTCCA TATTGGGAAT ACATATTGGT TGGATCCAAT GGTGCAAAGA 
AGCAAACTGT CTGGCTTTGC AGCTTGTATA AATGAATTAA ATGTGCGCCA TGAGATCTTG 
TCTACTGGGA TGGGAGTTGA TAATCCTCAG CACATAAGTC ATCTTGAGGA ATTATTGAAA 
AAAACTGGAG TAGCAACTGT GCAGCAAGAC TCTACTGATA AGGTATCAGA TGCAGACTTG 
TCAAGCAATA ATATGATCGC CAATTGCTGT GTTTCTTCTC TTTCCAGTTT GGATGCAAAC 
TGTGGTGCAA GGAATGTGGA CCTACAAAAT AAACTGGAGC GTAATCCACT AGAAAGTAAA 
AGCTTGCAAC CAGAAGTGAA ATGGTTTCAG AAAGATAAAG CAGTAATCCT GACGATAAAG 
TTACATGATG TTACAGATCA TGCCTGTATG TTTTATGCTT CCAGAGTTGT GTTTAGTTGT 
TATGCTGGAG GAAAGCACTA TGTGGCAGAT CTGGAACTTT GTGAGGAGAT ACTAAACGAA 
GAATGTGAGT GCTGCATTAA GAATGGAGAA CCTGTAATAA CATTATTGAA GGCTCAAAAC 
AGACCTTGGA AAAGCCTTCT GAAACAAAAG CATCCAAATG TGAGTCTTGA TTTTGACAAA 
TGGGAAGATT CTGAGGACAG TGATGGTTTT CCTTTTGTCT CCGGAAAGCC CAAGTGTCTT 
TACAAAGTTG TAAGTGAAGA AGTTGAATCT TCGGAATACA GTGAATCTGA CACTGAATCT 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tdrd12 ( XM_018093101.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948590.2 Xenopus tropicalis tudor domain containing 12 (tdrd12), transcript variant X1, mRNA.

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CloneID	OXa56928
Clone ID Related Accession (Same CDS sequence)	XM_031900806.1 , XM_018093101.2 , XM_012961356.3
Accession Version	XM_018093101.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	putative ATP-dependent RNA helicase TDRD12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018093101.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAAG TGGCAGTATT GCAGATTCAG GACCCCAGCT GCTTTTGGTG TGAAATATTA 
AAAAGCAATG GCCAAGATAT GAAGGAGAAA GTGGAATATG AGCAGCTGTA TAATGATCTT 
AATTGTCTTT ATGGAAAGGT CTACAGAAAT GTAGAGGAAA TAAAGCCAGC ATTTGTATCA 
GTTGGAGAGT TTTGCATGGT TTTTTGCGAA GACCTTAATA GTTGGTGCCG AGCTATCCTA 
AATGCACCTG CATGTAGTGC AGAAGATAAC CTTGTGGAGT GCTTCCTAGT GGATCATGCC 
ATTTACTGTC CTGTCAAAAA GAAAAACATT CGCCTTCCTG TGGAAGCTTG TCATCGTCTT 
CCTTTTCGTG CAAAAAAGTT TAAACTTCAC CAAATTCAGC CAGTATCTTT GTGTGTTGAT 
CTTTGTGGAG ATAAAGCAGA ACTTGGAGTT GCTGACAAAT GGGACGCTGC TGCTATTGAG 
TATTTTAAAC ATCTTCTAAA GGAGTCCGTT CATGCTGAAG CAAAATTATT CTCTATAGAA 
AACAATATTT TGAGTGTATA TCTTTACATC GTTACTACAG AGGCTACGGT GTGTGTTAAT 
GATGAACTGG TGGCAAAAAG CTTTGCTTGT TTCTGTTCTG ATGTACAATC TTGTGAAAAA 
ATCATTCCTA CAAGTCTACA AAATATGCAA CCTTCCAAGG AAGTTGATAA TCCTGCACAG 
ATGTTTTGGC CTGAGATCTA TAAAACAAAA AAAATACCAT TTTATGAAGG TTTTCCAATA 
AATGAAAAGG ATGAAATGGA GCCCAGAAAT CACTGTGAAA AGAAACCTGA AGAAAAAGAG 
ATGTGGGAAG AAAACCATGA CTTTGACAAA CTCCTGCAGA TTATCAATCC AGAACCCATA 
AAACTTTCTG AAAATGATTA CATACAGAAA CCAAGAACCA GTGCACCACA ATACCCAATT 
TTTGTGCGCA AAACTATCGA ACAGTGTTGT ACACTTAAAT TTACATCTCT GACGTCTGAT 
CTTAAAAAGG AGCTTGTGAG AAATCGTTAC TGTGGACCAA GTTTCACTGA ATCCTACAGC 
TGGCCTTCTG TAGCACAGGG ATTTAATACC ATTGTAATAT CACCTGATGG CATGAAACCC 
ATGAACTATA TTCCTCCTCT CCTCACATTT TTACACCTTG CTTCAGCAGT GTATACATTA 
CTGCCATCTA GAAATGGGCC TCTTGCAGCC ATTCTTTGCT CTGGATGGAA GAAAGCTCAT 
TTTGTACATG AACTACTTTT AAAGTACAGT AAACACACCA GACCCCTTAA TCCGATGTTG 
ATATTAGTTG GGCTAAAAGA GGAAGAATGC GAAAATCTTA ACATTCGAAA AGGATGTGAG 
GTGATTGTGA CCACACCACC TAGTCTTCTT AGATATCTGA AATGTAAGGG ATTGCTGCTG 
TTAAGGCTTT GTCATCTTGT CTTAGATGAA GTAGACATCC TTTTTTTAAA ATCAGGATCA 
CAGGTAATTG AAATTCTGGA ACTTTTTAAG AAATCAGTTG CAGCAGAAAG CCGAGAATGT 
GCACCTCAGC AGATTGTAGC TACTGGAACA TTTTGGCACA AGGAAATGAA TTCTTTACTG 
CAATATACAA CAGAACCTCA GGTCATAATC ACCAGAATGG AACAGGCTGC AGTGTATGGC 
AATGTCCAGC AGGTGATACA ACTTTGTTTA GACTGTGAAA AAATATCGGT ACTGCTACGG 
AATTTGGATT TCACACCAGA CCATGCTCAA AAGGTCTTGA TATTTGTTGA ATCTGATGAA 
GAGGCTGAGC TGGTTCACAA GGCTGTCCAA AACCATTCCA TTTTCAGTTT AGTTGTAAAC 
AGGAAGCAAG TGCAAAGTTT TAGCAGTGTG TTACAACAAT GGAAAAATAT GTTAAGCCGT 
GGAACACACA TTGTTTTAGT TGTTACAAAT GAATATTTAC CTCTTCTTGA AGTGACAGAT 
GCCACATGTA TAATACACTT CAGTTTTCCA GAAAATGAAA ATGTTTTTGG TCTGCGCCTC 
TATTCCTTAC TGGACTACAT ACACAGTAAA ACAGAGCGGG TAATTTCTTT AGAAGACAGT 
TTCCCAGTAG CCAGATCTGT TTTGCTTATG ACAGAAAAAC ATATACACCA TTCAACTGAT 
ATACTGCGTT ACTTACAACA CACAGAGGCC ACAATTCCGC CAGAGTTAAG TGATCTTACG 
CAAGGAATTT TACAGGCAAA TGAGAATAGG AAGTTTGATA AAGATATTTG TCAGCATATC 
AAAACTTTTG GTGTTTGCAG GTTAAATCTA TTTACATGCC CTGATCGCCA TAACCTCCTT 
CCATCTACAG ACCATCAGTA TTCCATGACA ACAACCAACC AGTACATATC TGTTGTGCCA 
GTACATGTTT TGGATCCAGT TCACTTTTTT GGAAGAATAG TAACTAAAGA GGATGTGTAT 
ACTCATCTAC TTGAAGATCT GAATAAATTT TACCAAATAC CAGGCAATCA AATATTAGTC 
CAGGAAGTAA ACGTTTCACA ATTATATGCT GTACAAGAGG GATCTGTTTA TCACAGGGTT 
AAAGTATTTT CTGCCAAAAA GGAGAATGGA GTTTTGAATT TTAGTTTGCA ATTCATTGAT 
GATGGAAGAA CTGATAAAGT TAGAGATGCC CAACTGTTGC AGTTACCTTC TCAATTTGAA 
AAAATTCCAC CACAGTCAGC TGAGTTTATT GTATGCAGGG TTCAACCAAT TGATAATGAG 
GCTGAATGGG ATCCCATTGT AACAAGGACC ATTAGTAAAG CCATAAAAGG AAAACTTCAT 
ACTGCAAAAG TTGTACTCCA TATTGGGAAT ACATATTGGT TGGATCCAAT GGTGCAAAGA 
AGCAAACTGT CTGGCTTTGC AGCTTGTATA AATGAATTAA ATGTGCGCCA TGAGATCTTG 
TCTACTGGGA TGGGAGTTGA TAATCCTCAG CACATAAGTC ATCTTGAGGA ATTATTGAAA 
AAAACTGGAG TAGCAACTGT GCAGCAAGAC TCTACTGATA AGGTATCAGA TGCAGACTTG 
TCAAGCAATA ATATGATCGC CAATTGCTGT GTTTCTTCTC TTTCCAGTTT GGATGCAAAC 
TGTGGTGCAA GGAATGTGGA CCTACAAAAT AAACTGGAGC GTAATCCACT AGAAAGTAAA 
AGCTTGCAAC CAGAAGTGAA ATGGTTTCAG AAAGATAAAG CAGTAATCCT GACGATAAAG 
TTACATGATG TTACAGATCA TGCCTGTATG TTTTATGCTT CCAGAGTTGT GTTTAGTTGT 
TATGCTGGAG GAAAGCACTA TGTGGCAGAT CTGGAACTTT GTGAGGAGAT ACTAAACGAA 
GAATGTGAGT GCTGCATTAA GAATGGAGAA CCTGTAATAA CATTATTGAA GGCTCAAAAC 
AGACCTTGGA AAAGCCTTCT GAAACAAAAG CATCCAAATG TGAGTCTTGA TTTTGACAAA 
TGGGAAGATT CTGAGGACAG TGATGGTTTT CCTTTTGTCT CCGGAAAGCC CAAGTGTCTT 
TACAAAGTTG TAAGTGAAGA AGTTGAATCT TCGGAATACA GTGAATCTGA CACTGAATCT 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948590.2
CDS	179..3784
Translation	MYEVAVLQIQDPSCFWCEILKSNGQDMKEKVEYEQLYNDLNCLYGKVYRNVEEIKPAFVSVGEFCMVFCEDLNSWCRAILNAPACSAEDNLVECFLVDHAIYCPVKKKNIRLPVEACHRLPFRAKKFKLHQIQPVSLCVDLCGDKAELGVADKWDAAAIEYFKHLLKESVHAEAKLFSIENNILSVYLYIVTTEATVCVNDELVAKSFACFCSDVQSCEKIIPTSLQNMQPSKEVDNPAQMFWPEIYKTKKIPFYEGFPINEKDEMEPRNHCEKKPEEKEMWEENHDFDKLLQIINPEPIKLSENDYIQKPRTSAPQYPIFVRKTIEQCCTLKFTSLTSDLKKELVRNRYCGPSFTESYSWPSVAQGFNTIVISPDGMKPMNYIPPLLTFLHLASAVYTLLPSRNGPLAAILCSGWKKAHFVHELLLKYSKHTRPLNPMLILVGLKEEECENLNIRKGCEVIVTTPPSLLRYLKCKGLLLLRLCHLVLDEVDILFLKSGSQVIEILELFKKSVAAESRECAPQQIVATGTFWHKEMNSLLQYTTEPQVIITRMEQAAVYGNVQQVIQLCLDCEKISVLLRNLDFTPDHAQKVLIFVESDEEAELVHKAVQNHSIFSLVVNRKQVQSFSSVLQQWKNMLSRGTHIVLVVTNEYLPLLEVTDATCIIHFSFPENENVFGLRLYSLLDYIHSKTERVISLEDSFPVARSVLLMTEKHIHHSTDILRYLQHTEATIPPELSDLTQGILQANENRKFDKDICQHIKTFGVCRLNLFTCPDRHNLLPSTDHQYSMTTTNQYISVVPVHVLDPVHFFGRIVTKEDVYTHLLEDLNKFYQIPGNQILVQEVNVSQLYAVQEGSVYHRVKVFSAKKENGVLNFSLQFIDDGRTDKVRDAQLLQLPSQFEKIPPQSAEFIVCRVQPIDNEAEWDPIVTRTISKAIKGKLHTAKVVLHIGNTYWLDPMVQRSKLSGFAACINELNVRHEILSTGMGVDNPQHISHLEELLKKTGVATVQQDSTDKVSDADLSSNNMIANCCVSSLSSLDANCGARNVDLQNKLERNPLESKSLQPEVKWFQKDKAVILTIKLHDVTDHACMFYASRVVFSCYAGGKHYVADLELCEEILNEECECCIKNGEPVITLLKAQNRPWKSLLKQKHPNVSLDFDKWEDSEDSDGFPFVSGKPKCLYKVVSEEVESSEYSESDTESD

Target ORF information:

RefSeq Version	XM_018093101.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tudor domain containing 12 (tdrd12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093101.2

ORF Insert Sequence:

ATGTATGAAG TGGCAGTATT GCAGATTCAG GACCCCAGCT GCTTTTGGTG TGAAATATTA 
AAAAGCAATG GCCAAGATAT GAAGGAGAAA GTGGAATATG AGCAGCTGTA TAATGATCTT 
AATTGTCTTT ATGGAAAGGT CTACAGAAAT GTAGAGGAAA TAAAGCCAGC ATTTGTATCA 
GTTGGAGAGT TTTGCATGGT TTTTTGCGAA GACCTTAATA GTTGGTGCCG AGCTATCCTA 
AATGCACCTG CATGTAGTGC AGAAGATAAC CTTGTGGAGT GCTTCCTAGT GGATCATGCC 
ATTTACTGTC CTGTCAAAAA GAAAAACATT CGCCTTCCTG TGGAAGCTTG TCATCGTCTT 
CCTTTTCGTG CAAAAAAGTT TAAACTTCAC CAAATTCAGC CAGTATCTTT GTGTGTTGAT 
CTTTGTGGAG ATAAAGCAGA ACTTGGAGTT GCTGACAAAT GGGACGCTGC TGCTATTGAG 
TATTTTAAAC ATCTTCTAAA GGAGTCCGTT CATGCTGAAG CAAAATTATT CTCTATAGAA 
AACAATATTT TGAGTGTATA TCTTTACATC GTTACTACAG AGGCTACGGT GTGTGTTAAT 
GATGAACTGG TGGCAAAAAG CTTTGCTTGT TTCTGTTCTG ATGTACAATC TTGTGAAAAA 
ATCATTCCTA CAAGTCTACA AAATATGCAA CCTTCCAAGG AAGTTGATAA TCCTGCACAG 
ATGTTTTGGC CTGAGATCTA TAAAACAAAA AAAATACCAT TTTATGAAGG TTTTCCAATA 
AATGAAAAGG ATGAAATGGA GCCCAGAAAT CACTGTGAAA AGAAACCTGA AGAAAAAGAG 
ATGTGGGAAG AAAACCATGA CTTTGACAAA CTCCTGCAGA TTATCAATCC AGAACCCATA 
AAACTTTCTG AAAATGATTA CATACAGAAA CCAAGAACCA GTGCACCACA ATACCCAATT 
TTTGTGCGCA AAACTATCGA ACAGTGTTGT ACACTTAAAT TTACATCTCT GACGTCTGAT 
CTTAAAAAGG AGCTTGTGAG AAATCGTTAC TGTGGACCAA GTTTCACTGA ATCCTACAGC 
TGGCCTTCTG TAGCACAGGG ATTTAATACC ATTGTAATAT CACCTGATGG CATGAAACCC 
ATGAACTATA TTCCTCCTCT CCTCACATTT TTACACCTTG CTTCAGCAGT GTATACATTA 
CTGCCATCTA GAAATGGGCC TCTTGCAGCC ATTCTTTGCT CTGGATGGAA GAAAGCTCAT 
TTTGTACATG AACTACTTTT AAAGTACAGT AAACACACCA GACCCCTTAA TCCGATGTTG 
ATATTAGTTG GGCTAAAAGA GGAAGAATGC GAAAATCTTA ACATTCGAAA AGGATGTGAG 
GTGATTGTGA CCACACCACC TAGTCTTCTT AGATATCTGA AATGTAAGGG ATTGCTGCTG 
TTAAGGCTTT GTCATCTTGT CTTAGATGAA GTAGACATCC TTTTTTTAAA ATCAGGATCA 
CAGGTAATTG AAATTCTGGA ACTTTTTAAG AAATCAGTTG CAGCAGAAAG CCGAGAATGT 
GCACCTCAGC AGATTGTAGC TACTGGAACA TTTTGGCACA AGGAAATGAA TTCTTTACTG 
CAATATACAA CAGAACCTCA GGTCATAATC ACCAGAATGG AACAGGCTGC AGTGTATGGC 
AATGTCCAGC AGGTGATACA ACTTTGTTTA GACTGTGAAA AAATATCGGT ACTGCTACGG 
AATTTGGATT TCACACCAGA CCATGCTCAA AAGGTCTTGA TATTTGTTGA ATCTGATGAA 
GAGGCTGAGC TGGTTCACAA GGCTGTCCAA AACCATTCCA TTTTCAGTTT AGTTGTAAAC 
AGGAAGCAAG TGCAAAGTTT TAGCAGTGTG TTACAACAAT GGAAAAATAT GTTAAGCCGT 
GGAACACACA TTGTTTTAGT TGTTACAAAT GAATATTTAC CTCTTCTTGA AGTGACAGAT 
GCCACATGTA TAATACACTT CAGTTTTCCA GAAAATGAAA ATGTTTTTGG TCTGCGCCTC 
TATTCCTTAC TGGACTACAT ACACAGTAAA ACAGAGCGGG TAATTTCTTT AGAAGACAGT 
TTCCCAGTAG CCAGATCTGT TTTGCTTATG ACAGAAAAAC ATATACACCA TTCAACTGAT 
ATACTGCGTT ACTTACAACA CACAGAGGCC ACAATTCCGC CAGAGTTAAG TGATCTTACG 
CAAGGAATTT TACAGGCAAA TGAGAATAGG AAGTTTGATA AAGATATTTG TCAGCATATC 
AAAACTTTTG GTGTTTGCAG GTTAAATCTA TTTACATGCC CTGATCGCCA TAACCTCCTT 
CCATCTACAG ACCATCAGTA TTCCATGACA ACAACCAACC AGTACATATC TGTTGTGCCA 
GTACATGTTT TGGATCCAGT TCACTTTTTT GGAAGAATAG TAACTAAAGA GGATGTGTAT 
ACTCATCTAC TTGAAGATCT GAATAAATTT TACCAAATAC CAGGCAATCA AATATTAGTC 
CAGGAAGTAA ACGTTTCACA ATTATATGCT GTACAAGAGG GATCTGTTTA TCACAGGGTT 
AAAGTATTTT CTGCCAAAAA GGAGAATGGA GTTTTGAATT TTAGTTTGCA ATTCATTGAT 
GATGGAAGAA CTGATAAAGT TAGAGATGCC CAACTGTTGC AGTTACCTTC TCAATTTGAA 
AAAATTCCAC CACAGTCAGC TGAGTTTATT GTATGCAGGG TTCAACCAAT TGATAATGAG 
GCTGAATGGG ATCCCATTGT AACAAGGACC ATTAGTAAAG CCATAAAAGG AAAACTTCAT 
ACTGCAAAAG TTGTACTCCA TATTGGGAAT ACATATTGGT TGGATCCAAT GGTGCAAAGA 
AGCAAACTGT CTGGCTTTGC AGCTTGTATA AATGAATTAA ATGTGCGCCA TGAGATCTTG 
TCTACTGGGA TGGGAGTTGA TAATCCTCAG CACATAAGTC ATCTTGAGGA ATTATTGAAA 
AAAACTGGAG TAGCAACTGT GCAGCAAGAC TCTACTGATA AGGTATCAGA TGCAGACTTG 
TCAAGCAATA ATATGATCGC CAATTGCTGT GTTTCTTCTC TTTCCAGTTT GGATGCAAAC 
TGTGGTGCAA GGAATGTGGA CCTACAAAAT AAACTGGAGC GTAATCCACT AGAAAGTAAA 
AGCTTGCAAC CAGAAGTGAA ATGGTTTCAG AAAGATAAAG CAGTAATCCT GACGATAAAG 
TTACATGATG TTACAGATCA TGCCTGTATG TTTTATGCTT CCAGAGTTGT GTTTAGTTGT 
TATGCTGGAG GAAAGCACTA TGTGGCAGAT CTGGAACTTT GTGAGGAGAT ACTAAACGAA 
GAATGTGAGT GCTGCATTAA GAATGGAGAA CCTGTAATAA CATTATTGAA GGCTCAAAAC 
AGACCTTGGA AAAGCCTTCT GAAACAAAAG CATCCAAATG TGAGTCTTGA TTTTGACAAA 
TGGGAAGATT CTGAGGACAG TGATGGTTTT CCTTTTGTCT CCGGAAAGCC CAAGTGTCTT 
TACAAAGTTG TAAGTGAAGA AGTTGAATCT TCGGAATACA GTGAATCTGA CACTGAATCT 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tdrd12 ( XM_012961356.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816810.2 Xenopus tropicalis tudor domain containing 12 (tdrd12), transcript variant X2, mRNA.

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CloneID	OXa56928
Clone ID Related Accession (Same CDS sequence)	XM_031900806.1 , XM_018093101.2 , XM_012961356.3
Accession Version	XM_012961356.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	putative ATP-dependent RNA helicase TDRD12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961356.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAAG TGGCAGTATT GCAGATTCAG GACCCCAGCT GCTTTTGGTG TGAAATATTA 
AAAAGCAATG GCCAAGATAT GAAGGAGAAA GTGGAATATG AGCAGCTGTA TAATGATCTT 
AATTGTCTTT ATGGAAAGGT CTACAGAAAT GTAGAGGAAA TAAAGCCAGC ATTTGTATCA 
GTTGGAGAGT TTTGCATGGT TTTTTGCGAA GACCTTAATA GTTGGTGCCG AGCTATCCTA 
AATGCACCTG CATGTAGTGC AGAAGATAAC CTTGTGGAGT GCTTCCTAGT GGATCATGCC 
ATTTACTGTC CTGTCAAAAA GAAAAACATT CGCCTTCCTG TGGAAGCTTG TCATCGTCTT 
CCTTTTCGTG CAAAAAAGTT TAAACTTCAC CAAATTCAGC CAGTATCTTT GTGTGTTGAT 
CTTTGTGGAG ATAAAGCAGA ACTTGGAGTT GCTGACAAAT GGGACGCTGC TGCTATTGAG 
TATTTTAAAC ATCTTCTAAA GGAGTCCGTT CATGCTGAAG CAAAATTATT CTCTATAGAA 
AACAATATTT TGAGTGTATA TCTTTACATC GTTACTACAG AGGCTACGGT GTGTGTTAAT 
GATGAACTGG TGGCAAAAAG CTTTGCTTGT TTCTGTTCTG ATGTACAATC TTGTGAAAAA 
ATCATTCCTA CAAGTCTACA AAATATGCAA CCTTCCAAGG AAGTTGATAA TCCTGCACAG 
ATGTTTTGGC CTGAGATCTA TAAAACAAAA AAAATACCAT TTTATGAAGG TTTTCCAATA 
AATGAAAAGG ATGAAATGGA GCCCAGAAAT CACTGTGAAA AGAAACCTGA AGAAAAAGAG 
ATGTGGGAAG AAAACCATGA CTTTGACAAA CTCCTGCAGA TTATCAATCC AGAACCCATA 
AAACTTTCTG AAAATGATTA CATACAGAAA CCAAGAACCA GTGCACCACA ATACCCAATT 
TTTGTGCGCA AAACTATCGA ACAGTGTTGT ACACTTAAAT TTACATCTCT GACGTCTGAT 
CTTAAAAAGG AGCTTGTGAG AAATCGTTAC TGTGGACCAA GTTTCACTGA ATCCTACAGC 
TGGCCTTCTG TAGCACAGGG ATTTAATACC ATTGTAATAT CACCTGATGG CATGAAACCC 
ATGAACTATA TTCCTCCTCT CCTCACATTT TTACACCTTG CTTCAGCAGT GTATACATTA 
CTGCCATCTA GAAATGGGCC TCTTGCAGCC ATTCTTTGCT CTGGATGGAA GAAAGCTCAT 
TTTGTACATG AACTACTTTT AAAGTACAGT AAACACACCA GACCCCTTAA TCCGATGTTG 
ATATTAGTTG GGCTAAAAGA GGAAGAATGC GAAAATCTTA ACATTCGAAA AGGATGTGAG 
GTGATTGTGA CCACACCACC TAGTCTTCTT AGATATCTGA AATGTAAGGG ATTGCTGCTG 
TTAAGGCTTT GTCATCTTGT CTTAGATGAA GTAGACATCC TTTTTTTAAA ATCAGGATCA 
CAGGTAATTG AAATTCTGGA ACTTTTTAAG AAATCAGTTG CAGCAGAAAG CCGAGAATGT 
GCACCTCAGC AGATTGTAGC TACTGGAACA TTTTGGCACA AGGAAATGAA TTCTTTACTG 
CAATATACAA CAGAACCTCA GGTCATAATC ACCAGAATGG AACAGGCTGC AGTGTATGGC 
AATGTCCAGC AGGTGATACA ACTTTGTTTA GACTGTGAAA AAATATCGGT ACTGCTACGG 
AATTTGGATT TCACACCAGA CCATGCTCAA AAGGTCTTGA TATTTGTTGA ATCTGATGAA 
GAGGCTGAGC TGGTTCACAA GGCTGTCCAA AACCATTCCA TTTTCAGTTT AGTTGTAAAC 
AGGAAGCAAG TGCAAAGTTT TAGCAGTGTG TTACAACAAT GGAAAAATAT GTTAAGCCGT 
GGAACACACA TTGTTTTAGT TGTTACAAAT GAATATTTAC CTCTTCTTGA AGTGACAGAT 
GCCACATGTA TAATACACTT CAGTTTTCCA GAAAATGAAA ATGTTTTTGG TCTGCGCCTC 
TATTCCTTAC TGGACTACAT ACACAGTAAA ACAGAGCGGG TAATTTCTTT AGAAGACAGT 
TTCCCAGTAG CCAGATCTGT TTTGCTTATG ACAGAAAAAC ATATACACCA TTCAACTGAT 
ATACTGCGTT ACTTACAACA CACAGAGGCC ACAATTCCGC CAGAGTTAAG TGATCTTACG 
CAAGGAATTT TACAGGCAAA TGAGAATAGG AAGTTTGATA AAGATATTTG TCAGCATATC 
AAAACTTTTG GTGTTTGCAG GTTAAATCTA TTTACATGCC CTGATCGCCA TAACCTCCTT 
CCATCTACAG ACCATCAGTA TTCCATGACA ACAACCAACC AGTACATATC TGTTGTGCCA 
GTACATGTTT TGGATCCAGT TCACTTTTTT GGAAGAATAG TAACTAAAGA GGATGTGTAT 
ACTCATCTAC TTGAAGATCT GAATAAATTT TACCAAATAC CAGGCAATCA AATATTAGTC 
CAGGAAGTAA ACGTTTCACA ATTATATGCT GTACAAGAGG GATCTGTTTA TCACAGGGTT 
AAAGTATTTT CTGCCAAAAA GGAGAATGGA GTTTTGAATT TTAGTTTGCA ATTCATTGAT 
GATGGAAGAA CTGATAAAGT TAGAGATGCC CAACTGTTGC AGTTACCTTC TCAATTTGAA 
AAAATTCCAC CACAGTCAGC TGAGTTTATT GTATGCAGGG TTCAACCAAT TGATAATGAG 
GCTGAATGGG ATCCCATTGT AACAAGGACC ATTAGTAAAG CCATAAAAGG AAAACTTCAT 
ACTGCAAAAG TTGTACTCCA TATTGGGAAT ACATATTGGT TGGATCCAAT GGTGCAAAGA 
AGCAAACTGT CTGGCTTTGC AGCTTGTATA AATGAATTAA ATGTGCGCCA TGAGATCTTG 
TCTACTGGGA TGGGAGTTGA TAATCCTCAG CACATAAGTC ATCTTGAGGA ATTATTGAAA 
AAAACTGGAG TAGCAACTGT GCAGCAAGAC TCTACTGATA AGGTATCAGA TGCAGACTTG 
TCAAGCAATA ATATGATCGC CAATTGCTGT GTTTCTTCTC TTTCCAGTTT GGATGCAAAC 
TGTGGTGCAA GGAATGTGGA CCTACAAAAT AAACTGGAGC GTAATCCACT AGAAAGTAAA 
AGCTTGCAAC CAGAAGTGAA ATGGTTTCAG AAAGATAAAG CAGTAATCCT GACGATAAAG 
TTACATGATG TTACAGATCA TGCCTGTATG TTTTATGCTT CCAGAGTTGT GTTTAGTTGT 
TATGCTGGAG GAAAGCACTA TGTGGCAGAT CTGGAACTTT GTGAGGAGAT ACTAAACGAA 
GAATGTGAGT GCTGCATTAA GAATGGAGAA CCTGTAATAA CATTATTGAA GGCTCAAAAC 
AGACCTTGGA AAAGCCTTCT GAAACAAAAG CATCCAAATG TGAGTCTTGA TTTTGACAAA 
TGGGAAGATT CTGAGGACAG TGATGGTTTT CCTTTTGTCT CCGGAAAGCC CAAGTGTCTT 
TACAAAGTTG TAAGTGAAGA AGTTGAATCT TCGGAATACA GTGAATCTGA CACTGAATCT 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816810.2
CDS	151..3756
Translation	MYEVAVLQIQDPSCFWCEILKSNGQDMKEKVEYEQLYNDLNCLYGKVYRNVEEIKPAFVSVGEFCMVFCEDLNSWCRAILNAPACSAEDNLVECFLVDHAIYCPVKKKNIRLPVEACHRLPFRAKKFKLHQIQPVSLCVDLCGDKAELGVADKWDAAAIEYFKHLLKESVHAEAKLFSIENNILSVYLYIVTTEATVCVNDELVAKSFACFCSDVQSCEKIIPTSLQNMQPSKEVDNPAQMFWPEIYKTKKIPFYEGFPINEKDEMEPRNHCEKKPEEKEMWEENHDFDKLLQIINPEPIKLSENDYIQKPRTSAPQYPIFVRKTIEQCCTLKFTSLTSDLKKELVRNRYCGPSFTESYSWPSVAQGFNTIVISPDGMKPMNYIPPLLTFLHLASAVYTLLPSRNGPLAAILCSGWKKAHFVHELLLKYSKHTRPLNPMLILVGLKEEECENLNIRKGCEVIVTTPPSLLRYLKCKGLLLLRLCHLVLDEVDILFLKSGSQVIEILELFKKSVAAESRECAPQQIVATGTFWHKEMNSLLQYTTEPQVIITRMEQAAVYGNVQQVIQLCLDCEKISVLLRNLDFTPDHAQKVLIFVESDEEAELVHKAVQNHSIFSLVVNRKQVQSFSSVLQQWKNMLSRGTHIVLVVTNEYLPLLEVTDATCIIHFSFPENENVFGLRLYSLLDYIHSKTERVISLEDSFPVARSVLLMTEKHIHHSTDILRYLQHTEATIPPELSDLTQGILQANENRKFDKDICQHIKTFGVCRLNLFTCPDRHNLLPSTDHQYSMTTTNQYISVVPVHVLDPVHFFGRIVTKEDVYTHLLEDLNKFYQIPGNQILVQEVNVSQLYAVQEGSVYHRVKVFSAKKENGVLNFSLQFIDDGRTDKVRDAQLLQLPSQFEKIPPQSAEFIVCRVQPIDNEAEWDPIVTRTISKAIKGKLHTAKVVLHIGNTYWLDPMVQRSKLSGFAACINELNVRHEILSTGMGVDNPQHISHLEELLKKTGVATVQQDSTDKVSDADLSSNNMIANCCVSSLSSLDANCGARNVDLQNKLERNPLESKSLQPEVKWFQKDKAVILTIKLHDVTDHACMFYASRVVFSCYAGGKHYVADLELCEEILNEECECCIKNGEPVITLLKAQNRPWKSLLKQKHPNVSLDFDKWEDSEDSDGFPFVSGKPKCLYKVVSEEVESSEYSESDTESD

Target ORF information:

RefSeq Version	XM_012961356.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tudor domain containing 12 (tdrd12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961356.3

ORF Insert Sequence:

ATGTATGAAG TGGCAGTATT GCAGATTCAG GACCCCAGCT GCTTTTGGTG TGAAATATTA 
AAAAGCAATG GCCAAGATAT GAAGGAGAAA GTGGAATATG AGCAGCTGTA TAATGATCTT 
AATTGTCTTT ATGGAAAGGT CTACAGAAAT GTAGAGGAAA TAAAGCCAGC ATTTGTATCA 
GTTGGAGAGT TTTGCATGGT TTTTTGCGAA GACCTTAATA GTTGGTGCCG AGCTATCCTA 
AATGCACCTG CATGTAGTGC AGAAGATAAC CTTGTGGAGT GCTTCCTAGT GGATCATGCC 
ATTTACTGTC CTGTCAAAAA GAAAAACATT CGCCTTCCTG TGGAAGCTTG TCATCGTCTT 
CCTTTTCGTG CAAAAAAGTT TAAACTTCAC CAAATTCAGC CAGTATCTTT GTGTGTTGAT 
CTTTGTGGAG ATAAAGCAGA ACTTGGAGTT GCTGACAAAT GGGACGCTGC TGCTATTGAG 
TATTTTAAAC ATCTTCTAAA GGAGTCCGTT CATGCTGAAG CAAAATTATT CTCTATAGAA 
AACAATATTT TGAGTGTATA TCTTTACATC GTTACTACAG AGGCTACGGT GTGTGTTAAT 
GATGAACTGG TGGCAAAAAG CTTTGCTTGT TTCTGTTCTG ATGTACAATC TTGTGAAAAA 
ATCATTCCTA CAAGTCTACA AAATATGCAA CCTTCCAAGG AAGTTGATAA TCCTGCACAG 
ATGTTTTGGC CTGAGATCTA TAAAACAAAA AAAATACCAT TTTATGAAGG TTTTCCAATA 
AATGAAAAGG ATGAAATGGA GCCCAGAAAT CACTGTGAAA AGAAACCTGA AGAAAAAGAG 
ATGTGGGAAG AAAACCATGA CTTTGACAAA CTCCTGCAGA TTATCAATCC AGAACCCATA 
AAACTTTCTG AAAATGATTA CATACAGAAA CCAAGAACCA GTGCACCACA ATACCCAATT 
TTTGTGCGCA AAACTATCGA ACAGTGTTGT ACACTTAAAT TTACATCTCT GACGTCTGAT 
CTTAAAAAGG AGCTTGTGAG AAATCGTTAC TGTGGACCAA GTTTCACTGA ATCCTACAGC 
TGGCCTTCTG TAGCACAGGG ATTTAATACC ATTGTAATAT CACCTGATGG CATGAAACCC 
ATGAACTATA TTCCTCCTCT CCTCACATTT TTACACCTTG CTTCAGCAGT GTATACATTA 
CTGCCATCTA GAAATGGGCC TCTTGCAGCC ATTCTTTGCT CTGGATGGAA GAAAGCTCAT 
TTTGTACATG AACTACTTTT AAAGTACAGT AAACACACCA GACCCCTTAA TCCGATGTTG 
ATATTAGTTG GGCTAAAAGA GGAAGAATGC GAAAATCTTA ACATTCGAAA AGGATGTGAG 
GTGATTGTGA CCACACCACC TAGTCTTCTT AGATATCTGA AATGTAAGGG ATTGCTGCTG 
TTAAGGCTTT GTCATCTTGT CTTAGATGAA GTAGACATCC TTTTTTTAAA ATCAGGATCA 
CAGGTAATTG AAATTCTGGA ACTTTTTAAG AAATCAGTTG CAGCAGAAAG CCGAGAATGT 
GCACCTCAGC AGATTGTAGC TACTGGAACA TTTTGGCACA AGGAAATGAA TTCTTTACTG 
CAATATACAA CAGAACCTCA GGTCATAATC ACCAGAATGG AACAGGCTGC AGTGTATGGC 
AATGTCCAGC AGGTGATACA ACTTTGTTTA GACTGTGAAA AAATATCGGT ACTGCTACGG 
AATTTGGATT TCACACCAGA CCATGCTCAA AAGGTCTTGA TATTTGTTGA ATCTGATGAA 
GAGGCTGAGC TGGTTCACAA GGCTGTCCAA AACCATTCCA TTTTCAGTTT AGTTGTAAAC 
AGGAAGCAAG TGCAAAGTTT TAGCAGTGTG TTACAACAAT GGAAAAATAT GTTAAGCCGT 
GGAACACACA TTGTTTTAGT TGTTACAAAT GAATATTTAC CTCTTCTTGA AGTGACAGAT 
GCCACATGTA TAATACACTT CAGTTTTCCA GAAAATGAAA ATGTTTTTGG TCTGCGCCTC 
TATTCCTTAC TGGACTACAT ACACAGTAAA ACAGAGCGGG TAATTTCTTT AGAAGACAGT 
TTCCCAGTAG CCAGATCTGT TTTGCTTATG ACAGAAAAAC ATATACACCA TTCAACTGAT 
ATACTGCGTT ACTTACAACA CACAGAGGCC ACAATTCCGC CAGAGTTAAG TGATCTTACG 
CAAGGAATTT TACAGGCAAA TGAGAATAGG AAGTTTGATA AAGATATTTG TCAGCATATC 
AAAACTTTTG GTGTTTGCAG GTTAAATCTA TTTACATGCC CTGATCGCCA TAACCTCCTT 
CCATCTACAG ACCATCAGTA TTCCATGACA ACAACCAACC AGTACATATC TGTTGTGCCA 
GTACATGTTT TGGATCCAGT TCACTTTTTT GGAAGAATAG TAACTAAAGA GGATGTGTAT 
ACTCATCTAC TTGAAGATCT GAATAAATTT TACCAAATAC CAGGCAATCA AATATTAGTC 
CAGGAAGTAA ACGTTTCACA ATTATATGCT GTACAAGAGG GATCTGTTTA TCACAGGGTT 
AAAGTATTTT CTGCCAAAAA GGAGAATGGA GTTTTGAATT TTAGTTTGCA ATTCATTGAT 
GATGGAAGAA CTGATAAAGT TAGAGATGCC CAACTGTTGC AGTTACCTTC TCAATTTGAA 
AAAATTCCAC CACAGTCAGC TGAGTTTATT GTATGCAGGG TTCAACCAAT TGATAATGAG 
GCTGAATGGG ATCCCATTGT AACAAGGACC ATTAGTAAAG CCATAAAAGG AAAACTTCAT 
ACTGCAAAAG TTGTACTCCA TATTGGGAAT ACATATTGGT TGGATCCAAT GGTGCAAAGA 
AGCAAACTGT CTGGCTTTGC AGCTTGTATA AATGAATTAA ATGTGCGCCA TGAGATCTTG 
TCTACTGGGA TGGGAGTTGA TAATCCTCAG CACATAAGTC ATCTTGAGGA ATTATTGAAA 
AAAACTGGAG TAGCAACTGT GCAGCAAGAC TCTACTGATA AGGTATCAGA TGCAGACTTG 
TCAAGCAATA ATATGATCGC CAATTGCTGT GTTTCTTCTC TTTCCAGTTT GGATGCAAAC 
TGTGGTGCAA GGAATGTGGA CCTACAAAAT AAACTGGAGC GTAATCCACT AGAAAGTAAA 
AGCTTGCAAC CAGAAGTGAA ATGGTTTCAG AAAGATAAAG CAGTAATCCT GACGATAAAG 
TTACATGATG TTACAGATCA TGCCTGTATG TTTTATGCTT CCAGAGTTGT GTTTAGTTGT 
TATGCTGGAG GAAAGCACTA TGTGGCAGAT CTGGAACTTT GTGAGGAGAT ACTAAACGAA 
GAATGTGAGT GCTGCATTAA GAATGGAGAA CCTGTAATAA CATTATTGAA GGCTCAAAAC 
AGACCTTGGA AAAGCCTTCT GAAACAAAAG CATCCAAATG TGAGTCTTGA TTTTGACAAA 
TGGGAAGATT CTGAGGACAG TGATGGTTTT CCTTTTGTCT CCGGAAAGCC CAAGTGTCTT 
TACAAAGTTG TAAGTGAAGA AGTTGAATCT TCGGAATACA GTGAATCTGA CACTGAATCT 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tdrd12 ( XM_018093101.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948590.1 Xenopus tropicalis tudor domain containing 12 (tdrd12), transcript variant X1, mRNA.

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CloneID	OXa29419
Clone ID Related Accession (Same CDS sequence)	XM_018093101.1 , XM_012961356.2
Accession Version	XM_018093101.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	putative ATP-dependent RNA helicase TDRD12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAAG TGGCAGTATT GCAGATTCAG GACCCCAGCT GCTTTTGGTG TGAAATATTA 
AAAAGCAATG GCCAAGATAT GAAGGAGAAA GTGGAATATG AGCAGCTGTA TAATGATCTT 
AATTGTCTTT ATGGAAAGGT CTACAGAAAT GTAGAGGAAA TAAAGCCAGC ATTTGTATCA 
GTTGGAGAGT TTTGCATGGT TTTTTGCGAA GACCTTAATA GTTGGTGCCG AGCTATCCTA 
AATGCACCTG CATGTAGTGC AGAAGATAAC CTTGTGGAGT GCTTCCTAGT GGATCATGCC 
ATTTACTGTC CTGTCAAAAA GAAAAACATT CGCCTTCCTG TGGAAGCTTG TCATCGTCTT 
CCTTTTCGTG CAAAAAAGTT TAAACTTCAC CAAATTCAGC CAGTATCTTT GTGTGTTGAT 
CTTTGTGGAG ATAAAGCAGA ACTTGGAGTT GCTGACAAAT GGGACGCTGC TGCTATTGAG 
TATTTTAAAC ATCTTCTAAA GGAGTCCGTT CATGCTGAAG CAAAATTATT CTCTATAGAA 
AACAATATTT TGAGTGTATA TCTTTACATC GTTACTACAG AGGCTACGGT GTGTGTTAAT 
GATGAACTGG TGGCAAAAAG CTTTGCTTGT TTCTGTTCTG ATGTACAATC TTGTGAAAAA 
ATCATTCCTA CAAGTCTACA AAATATGCAA CCTTCCAAGG AAGTTGATAA TCCTGCACAG 
ATGTTTTGGC CTGAGATCTA TAAAACAAAA AAAATACCAT TTTATGAAGG TTTTCCAATA 
AATGAAAAGG ATGAAATGGA GCCCAGAAAT CACTGTGAAA AGAAACCTGA AGAAAAAGAG 
ATGTGGGAAG AAAACCATGA CTTTGACAAA CTCCTGCAGA TTATCAATCC AGAACCCATA 
AAACTTTCTG AAAATGATTA CATACAGAAA CCAAGAACCA GTGCACCACA ATACCCAATT 
TTTGTGCGCA AAACTATCGA ACAGTGTTGT ACACTTAAAT TTACATCTCT GACGTCTGAT 
CTTAAAAAGG AGCTTGTGAG AAATCGTTAC TGTGGACCAA GTTTCACTGA ATCCTACAGC 
TGGCCTTCTG TAGCACAGGG ATTTAATACC ATTGTAATAT CACCTGATGG CATGAAACCC 
ATGAACTATA TTCCTCCTCT CCTCACATTT TTACACCTTG CTTCAGCAGT GTATACATTA 
CTGCCATCTA GAAATGGGCC TCTTGCAGCC ATTCTTTGCT CTGGATGGAA GAAAGCTCAT 
TTTGTACATG AACTACTTTT AAAGTACAGT AAACACACCA GACCCCTTAA TCCGATGTTG 
ATATTAGTTG GGCTAAAAGA GGAAGAATGC GAAAATCTTA ACATTCGAAA AGGATGTGAG 
GTGATTGTGA CCACACCACC TAGTCTTCTT AGATATCTGA AATGTAAGGG ATTGCTGCTG 
TTAAGGCTTT GTCATCTTGT CTTAGATGAA GTAGACATCC TTTTTTTAAA ATCAGGATCA 
CAGGTAATTG AAATTCTGGA ACTTTTTAAG AAATCAGTTG CAGCAGAAAG CCGAGAATGT 
GCACCTCAGC AGATTGTAGC TACTGGAACA TTTTGGCACA AGGAAATGAA TTCTTTACTG 
CAATATACAA CAGAACCTCA GGTCATAATC ACCAGAATGG AACAGGCTGC AGTGTATGGC 
AATGTCCAGC AGGTGATACA ACTTTGTTTA AACTGTGAAA AAATATCGGT ACTGCTACGG 
AATTTGGATT TCACACCAGA CCATGCTCAA AAGGTCTTGA TATTTGTTGA ATCTGATGAA 
GAGGCTGAGC TGGTTCACAA GGCTGTCCAA AACCATTCCA TTTTCAGTTT AGTTGTAAAC 
AGGAAGCAAG TGCAAAGTTT TAGCAGTGTG TTACAACAAT GGAAAAATAT GTTAAGCCGT 
GGAACACACA TTGTTTTAGT TGTTACAAAT GAATATTTAC CTCTTCTTGA AGTGACAGAT 
GCCACATGTA TAATACACTT CAGTTTTCCA GAAAATGAAA ATGTTTTTGG TCTGCGCCTC 
TATTCCTTAC TGGACTACAT ACACAGTAAA ACAGAGCGGG TAATTTCTTT AGAAGACAGT 
TTCCCAGTAG CCAGATCTGT TTTGCTTATG ACAGAAAAAC ATATACACCA TTCAACTGAT 
ATACTGCGTT ACTTACAACA CACAGAGGCC ACAATTCCGC CAGAGTTAAG TGATCTTACG 
CAAGGAATTT TACAGGCAAA TGAGAATAGG AAGTTTGATA AAGATATTTG TCAGCATATC 
AAAACTTTTG GTGTTTGCAG GTTAAATCTA TTTACATGCC CTGATCGCCA TAACCTCCTT 
CCATCTACAG ACCATCAGTA TTCCATGACA ACAACCAACC AGTACATATC TGTTGTGCCA 
GTACATGTTT TGGATCCAGT TCACTTTTTT GGAAGAATAG TAACTAAAGA GGATGTGTAT 
ACTCATCTAC TTGAAGATCT GAATAAATTT TACCAAATAC CAGGCAATCA AATATTAGTC 
CAGGAAGTAA ACGTTTCACA ATTATATGCT GTACAAGAGG GATCTGTTTA TCACAGGGTT 
AAAGTATTTT CTGCCAAAAA GGAGAATGGA GTTTTGAATT TTAGTTTGCA ATTCATTGAT 
GATGGAAGAA CTGATAAAGT TAGAGATGCC CAACTGTTGC AGTTACCTTC TCAATTTGAA 
AAAATTCCAC CACAGTCAGC TGAGTTTATT GTATGCAGGG TTCAACCAAT TGATAATGAG 
GCTGAATGGG ATCCCATTGT AACAAGGACC ATTAGTAAAG CCATAAAAGG AAAACTTCAT 
ACTGCAAAAG TTGTACTCCA TATTGGGAAT ACATATTGGT TGGATCCAAT GGTGCAAAGA 
AGCAAACTGT CTGGCTTTGC AGCTTGTATA AATGAATTAA ATGTGCGCCA TGAGATCTTG 
TCTACTGGGA TGGGAGTTGA TAATCCTCAG CACATAAGTC ATCTTGAGGA ATTATTGAAA 
AAAACTGGAG TAGCAACTGT GCAGCAAGAC TCTACTGATA AGGTATCAGA TGCAGACTTG 
TCAAGCAATA ATATGATCGC CAATTGCTGT GTTTCTTCTC TTTCCAGTTT GGATGCAAAC 
TGTGGTGCAA GGAATGTGGA CCTACAAAAT AAACTGGAGC GTAATCCACT AGAAAGTAAA 
AGCTTGCAAC CAGAAGTGAA ATGGTTTCAG AAAGATAAAG CAGTAATCCT GACGATAAAG 
TTACATGATG TTACAGATCA TGCCTGTATG TTTTATGCTT CCAGAGTTGT GTTTAGTTGT 
TATGCTGGAG GAAAGCACTA TGTGGCAGAT CTGGAACTTT GTGAGGAGAT ACTAAACGAA 
GAATGTGAGT GCTGCATTAA GAATGGAGAA CCTGTAATAA CATTATTGAA GGCTCAAAAC 
AGACCTTGGA AAAGCCTTCT GAAACAAAAG CATCCAAATG TGAGTCTTGA TTTTGACAAA 
TGGGAAGATT CTGAGGACAG TGATGGTTTT CCTTTTGTCT CCGGAAAGCC CAAGTGTCTT 
TACAAAGTTG TAAGTGAAGA AGTTGAATCT TCGGAATACA GTGAATCTGA CACTGAATCT 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948590.1
CDS	187..3792
Translation	MYEVAVLQIQDPSCFWCEILKSNGQDMKEKVEYEQLYNDLNCLYGKVYRNVEEIKPAFVSVGEFCMVFCEDLNSWCRAILNAPACSAEDNLVECFLVDHAIYCPVKKKNIRLPVEACHRLPFRAKKFKLHQIQPVSLCVDLCGDKAELGVADKWDAAAIEYFKHLLKESVHAEAKLFSIENNILSVYLYIVTTEATVCVNDELVAKSFACFCSDVQSCEKIIPTSLQNMQPSKEVDNPAQMFWPEIYKTKKIPFYEGFPINEKDEMEPRNHCEKKPEEKEMWEENHDFDKLLQIINPEPIKLSENDYIQKPRTSAPQYPIFVRKTIEQCCTLKFTSLTSDLKKELVRNRYCGPSFTESYSWPSVAQGFNTIVISPDGMKPMNYIPPLLTFLHLASAVYTLLPSRNGPLAAILCSGWKKAHFVHELLLKYSKHTRPLNPMLILVGLKEEECENLNIRKGCEVIVTTPPSLLRYLKCKGLLLLRLCHLVLDEVDILFLKSGSQVIEILELFKKSVAAESRECAPQQIVATGTFWHKEMNSLLQYTTEPQVIITRMEQAAVYGNVQQVIQLCLNCEKISVLLRNLDFTPDHAQKVLIFVESDEEAELVHKAVQNHSIFSLVVNRKQVQSFSSVLQQWKNMLSRGTHIVLVVTNEYLPLLEVTDATCIIHFSFPENENVFGLRLYSLLDYIHSKTERVISLEDSFPVARSVLLMTEKHIHHSTDILRYLQHTEATIPPELSDLTQGILQANENRKFDKDICQHIKTFGVCRLNLFTCPDRHNLLPSTDHQYSMTTTNQYISVVPVHVLDPVHFFGRIVTKEDVYTHLLEDLNKFYQIPGNQILVQEVNVSQLYAVQEGSVYHRVKVFSAKKENGVLNFSLQFIDDGRTDKVRDAQLLQLPSQFEKIPPQSAEFIVCRVQPIDNEAEWDPIVTRTISKAIKGKLHTAKVVLHIGNTYWLDPMVQRSKLSGFAACINELNVRHEILSTGMGVDNPQHISHLEELLKKTGVATVQQDSTDKVSDADLSSNNMIANCCVSSLSSLDANCGARNVDLQNKLERNPLESKSLQPEVKWFQKDKAVILTIKLHDVTDHACMFYASRVVFSCYAGGKHYVADLELCEEILNEECECCIKNGEPVITLLKAQNRPWKSLLKQKHPNVSLDFDKWEDSEDSDGFPFVSGKPKCLYKVVSEEVESSEYSESDTESD

Target ORF information:

RefSeq Version	XM_018093101.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tudor domain containing 12 (tdrd12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093101.1

ORF Insert Sequence:

ATGTATGAAG TGGCAGTATT GCAGATTCAG GACCCCAGCT GCTTTTGGTG TGAAATATTA 
AAAAGCAATG GCCAAGATAT GAAGGAGAAA GTGGAATATG AGCAGCTGTA TAATGATCTT 
AATTGTCTTT ATGGAAAGGT CTACAGAAAT GTAGAGGAAA TAAAGCCAGC ATTTGTATCA 
GTTGGAGAGT TTTGCATGGT TTTTTGCGAA GACCTTAATA GTTGGTGCCG AGCTATCCTA 
AATGCACCTG CATGTAGTGC AGAAGATAAC CTTGTGGAGT GCTTCCTAGT GGATCATGCC 
ATTTACTGTC CTGTCAAAAA GAAAAACATT CGCCTTCCTG TGGAAGCTTG TCATCGTCTT 
CCTTTTCGTG CAAAAAAGTT TAAACTTCAC CAAATTCAGC CAGTATCTTT GTGTGTTGAT 
CTTTGTGGAG ATAAAGCAGA ACTTGGAGTT GCTGACAAAT GGGACGCTGC TGCTATTGAG 
TATTTTAAAC ATCTTCTAAA GGAGTCCGTT CATGCTGAAG CAAAATTATT CTCTATAGAA 
AACAATATTT TGAGTGTATA TCTTTACATC GTTACTACAG AGGCTACGGT GTGTGTTAAT 
GATGAACTGG TGGCAAAAAG CTTTGCTTGT TTCTGTTCTG ATGTACAATC TTGTGAAAAA 
ATCATTCCTA CAAGTCTACA AAATATGCAA CCTTCCAAGG AAGTTGATAA TCCTGCACAG 
ATGTTTTGGC CTGAGATCTA TAAAACAAAA AAAATACCAT TTTATGAAGG TTTTCCAATA 
AATGAAAAGG ATGAAATGGA GCCCAGAAAT CACTGTGAAA AGAAACCTGA AGAAAAAGAG 
ATGTGGGAAG AAAACCATGA CTTTGACAAA CTCCTGCAGA TTATCAATCC AGAACCCATA 
AAACTTTCTG AAAATGATTA CATACAGAAA CCAAGAACCA GTGCACCACA ATACCCAATT 
TTTGTGCGCA AAACTATCGA ACAGTGTTGT ACACTTAAAT TTACATCTCT GACGTCTGAT 
CTTAAAAAGG AGCTTGTGAG AAATCGTTAC TGTGGACCAA GTTTCACTGA ATCCTACAGC 
TGGCCTTCTG TAGCACAGGG ATTTAATACC ATTGTAATAT CACCTGATGG CATGAAACCC 
ATGAACTATA TTCCTCCTCT CCTCACATTT TTACACCTTG CTTCAGCAGT GTATACATTA 
CTGCCATCTA GAAATGGGCC TCTTGCAGCC ATTCTTTGCT CTGGATGGAA GAAAGCTCAT 
TTTGTACATG AACTACTTTT AAAGTACAGT AAACACACCA GACCCCTTAA TCCGATGTTG 
ATATTAGTTG GGCTAAAAGA GGAAGAATGC GAAAATCTTA ACATTCGAAA AGGATGTGAG 
GTGATTGTGA CCACACCACC TAGTCTTCTT AGATATCTGA AATGTAAGGG ATTGCTGCTG 
TTAAGGCTTT GTCATCTTGT CTTAGATGAA GTAGACATCC TTTTTTTAAA ATCAGGATCA 
CAGGTAATTG AAATTCTGGA ACTTTTTAAG AAATCAGTTG CAGCAGAAAG CCGAGAATGT 
GCACCTCAGC AGATTGTAGC TACTGGAACA TTTTGGCACA AGGAAATGAA TTCTTTACTG 
CAATATACAA CAGAACCTCA GGTCATAATC ACCAGAATGG AACAGGCTGC AGTGTATGGC 
AATGTCCAGC AGGTGATACA ACTTTGTTTA AACTGTGAAA AAATATCGGT ACTGCTACGG 
AATTTGGATT TCACACCAGA CCATGCTCAA AAGGTCTTGA TATTTGTTGA ATCTGATGAA 
GAGGCTGAGC TGGTTCACAA GGCTGTCCAA AACCATTCCA TTTTCAGTTT AGTTGTAAAC 
AGGAAGCAAG TGCAAAGTTT TAGCAGTGTG TTACAACAAT GGAAAAATAT GTTAAGCCGT 
GGAACACACA TTGTTTTAGT TGTTACAAAT GAATATTTAC CTCTTCTTGA AGTGACAGAT 
GCCACATGTA TAATACACTT CAGTTTTCCA GAAAATGAAA ATGTTTTTGG TCTGCGCCTC 
TATTCCTTAC TGGACTACAT ACACAGTAAA ACAGAGCGGG TAATTTCTTT AGAAGACAGT 
TTCCCAGTAG CCAGATCTGT TTTGCTTATG ACAGAAAAAC ATATACACCA TTCAACTGAT 
ATACTGCGTT ACTTACAACA CACAGAGGCC ACAATTCCGC CAGAGTTAAG TGATCTTACG 
CAAGGAATTT TACAGGCAAA TGAGAATAGG AAGTTTGATA AAGATATTTG TCAGCATATC 
AAAACTTTTG GTGTTTGCAG GTTAAATCTA TTTACATGCC CTGATCGCCA TAACCTCCTT 
CCATCTACAG ACCATCAGTA TTCCATGACA ACAACCAACC AGTACATATC TGTTGTGCCA 
GTACATGTTT TGGATCCAGT TCACTTTTTT GGAAGAATAG TAACTAAAGA GGATGTGTAT 
ACTCATCTAC TTGAAGATCT GAATAAATTT TACCAAATAC CAGGCAATCA AATATTAGTC 
CAGGAAGTAA ACGTTTCACA ATTATATGCT GTACAAGAGG GATCTGTTTA TCACAGGGTT 
AAAGTATTTT CTGCCAAAAA GGAGAATGGA GTTTTGAATT TTAGTTTGCA ATTCATTGAT 
GATGGAAGAA CTGATAAAGT TAGAGATGCC CAACTGTTGC AGTTACCTTC TCAATTTGAA 
AAAATTCCAC CACAGTCAGC TGAGTTTATT GTATGCAGGG TTCAACCAAT TGATAATGAG 
GCTGAATGGG ATCCCATTGT AACAAGGACC ATTAGTAAAG CCATAAAAGG AAAACTTCAT 
ACTGCAAAAG TTGTACTCCA TATTGGGAAT ACATATTGGT TGGATCCAAT GGTGCAAAGA 
AGCAAACTGT CTGGCTTTGC AGCTTGTATA AATGAATTAA ATGTGCGCCA TGAGATCTTG 
TCTACTGGGA TGGGAGTTGA TAATCCTCAG CACATAAGTC ATCTTGAGGA ATTATTGAAA 
AAAACTGGAG TAGCAACTGT GCAGCAAGAC TCTACTGATA AGGTATCAGA TGCAGACTTG 
TCAAGCAATA ATATGATCGC CAATTGCTGT GTTTCTTCTC TTTCCAGTTT GGATGCAAAC 
TGTGGTGCAA GGAATGTGGA CCTACAAAAT AAACTGGAGC GTAATCCACT AGAAAGTAAA 
AGCTTGCAAC CAGAAGTGAA ATGGTTTCAG AAAGATAAAG CAGTAATCCT GACGATAAAG 
TTACATGATG TTACAGATCA TGCCTGTATG TTTTATGCTT CCAGAGTTGT GTTTAGTTGT 
TATGCTGGAG GAAAGCACTA TGTGGCAGAT CTGGAACTTT GTGAGGAGAT ACTAAACGAA 
GAATGTGAGT GCTGCATTAA GAATGGAGAA CCTGTAATAA CATTATTGAA GGCTCAAAAC 
AGACCTTGGA AAAGCCTTCT GAAACAAAAG CATCCAAATG TGAGTCTTGA TTTTGACAAA 
TGGGAAGATT CTGAGGACAG TGATGGTTTT CCTTTTGTCT CCGGAAAGCC CAAGTGTCTT 
TACAAAGTTG TAAGTGAAGA AGTTGAATCT TCGGAATACA GTGAATCTGA CACTGAATCT 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tdrd12 ( XM_012961356.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816810.1 Xenopus tropicalis tudor domain containing 12 (tdrd12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa29419
Clone ID Related Accession (Same CDS sequence)	XM_018093101.1 , XM_012961356.2
Accession Version	XM_012961356.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	putative ATP-dependent RNA helicase TDRD12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961356.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAAG TGGCAGTATT GCAGATTCAG GACCCCAGCT GCTTTTGGTG TGAAATATTA 
AAAAGCAATG GCCAAGATAT GAAGGAGAAA GTGGAATATG AGCAGCTGTA TAATGATCTT 
AATTGTCTTT ATGGAAAGGT CTACAGAAAT GTAGAGGAAA TAAAGCCAGC ATTTGTATCA 
GTTGGAGAGT TTTGCATGGT TTTTTGCGAA GACCTTAATA GTTGGTGCCG AGCTATCCTA 
AATGCACCTG CATGTAGTGC AGAAGATAAC CTTGTGGAGT GCTTCCTAGT GGATCATGCC 
ATTTACTGTC CTGTCAAAAA GAAAAACATT CGCCTTCCTG TGGAAGCTTG TCATCGTCTT 
CCTTTTCGTG CAAAAAAGTT TAAACTTCAC CAAATTCAGC CAGTATCTTT GTGTGTTGAT 
CTTTGTGGAG ATAAAGCAGA ACTTGGAGTT GCTGACAAAT GGGACGCTGC TGCTATTGAG 
TATTTTAAAC ATCTTCTAAA GGAGTCCGTT CATGCTGAAG CAAAATTATT CTCTATAGAA 
AACAATATTT TGAGTGTATA TCTTTACATC GTTACTACAG AGGCTACGGT GTGTGTTAAT 
GATGAACTGG TGGCAAAAAG CTTTGCTTGT TTCTGTTCTG ATGTACAATC TTGTGAAAAA 
ATCATTCCTA CAAGTCTACA AAATATGCAA CCTTCCAAGG AAGTTGATAA TCCTGCACAG 
ATGTTTTGGC CTGAGATCTA TAAAACAAAA AAAATACCAT TTTATGAAGG TTTTCCAATA 
AATGAAAAGG ATGAAATGGA GCCCAGAAAT CACTGTGAAA AGAAACCTGA AGAAAAAGAG 
ATGTGGGAAG AAAACCATGA CTTTGACAAA CTCCTGCAGA TTATCAATCC AGAACCCATA 
AAACTTTCTG AAAATGATTA CATACAGAAA CCAAGAACCA GTGCACCACA ATACCCAATT 
TTTGTGCGCA AAACTATCGA ACAGTGTTGT ACACTTAAAT TTACATCTCT GACGTCTGAT 
CTTAAAAAGG AGCTTGTGAG AAATCGTTAC TGTGGACCAA GTTTCACTGA ATCCTACAGC 
TGGCCTTCTG TAGCACAGGG ATTTAATACC ATTGTAATAT CACCTGATGG CATGAAACCC 
ATGAACTATA TTCCTCCTCT CCTCACATTT TTACACCTTG CTTCAGCAGT GTATACATTA 
CTGCCATCTA GAAATGGGCC TCTTGCAGCC ATTCTTTGCT CTGGATGGAA GAAAGCTCAT 
TTTGTACATG AACTACTTTT AAAGTACAGT AAACACACCA GACCCCTTAA TCCGATGTTG 
ATATTAGTTG GGCTAAAAGA GGAAGAATGC GAAAATCTTA ACATTCGAAA AGGATGTGAG 
GTGATTGTGA CCACACCACC TAGTCTTCTT AGATATCTGA AATGTAAGGG ATTGCTGCTG 
TTAAGGCTTT GTCATCTTGT CTTAGATGAA GTAGACATCC TTTTTTTAAA ATCAGGATCA 
CAGGTAATTG AAATTCTGGA ACTTTTTAAG AAATCAGTTG CAGCAGAAAG CCGAGAATGT 
GCACCTCAGC AGATTGTAGC TACTGGAACA TTTTGGCACA AGGAAATGAA TTCTTTACTG 
CAATATACAA CAGAACCTCA GGTCATAATC ACCAGAATGG AACAGGCTGC AGTGTATGGC 
AATGTCCAGC AGGTGATACA ACTTTGTTTA AACTGTGAAA AAATATCGGT ACTGCTACGG 
AATTTGGATT TCACACCAGA CCATGCTCAA AAGGTCTTGA TATTTGTTGA ATCTGATGAA 
GAGGCTGAGC TGGTTCACAA GGCTGTCCAA AACCATTCCA TTTTCAGTTT AGTTGTAAAC 
AGGAAGCAAG TGCAAAGTTT TAGCAGTGTG TTACAACAAT GGAAAAATAT GTTAAGCCGT 
GGAACACACA TTGTTTTAGT TGTTACAAAT GAATATTTAC CTCTTCTTGA AGTGACAGAT 
GCCACATGTA TAATACACTT CAGTTTTCCA GAAAATGAAA ATGTTTTTGG TCTGCGCCTC 
TATTCCTTAC TGGACTACAT ACACAGTAAA ACAGAGCGGG TAATTTCTTT AGAAGACAGT 
TTCCCAGTAG CCAGATCTGT TTTGCTTATG ACAGAAAAAC ATATACACCA TTCAACTGAT 
ATACTGCGTT ACTTACAACA CACAGAGGCC ACAATTCCGC CAGAGTTAAG TGATCTTACG 
CAAGGAATTT TACAGGCAAA TGAGAATAGG AAGTTTGATA AAGATATTTG TCAGCATATC 
AAAACTTTTG GTGTTTGCAG GTTAAATCTA TTTACATGCC CTGATCGCCA TAACCTCCTT 
CCATCTACAG ACCATCAGTA TTCCATGACA ACAACCAACC AGTACATATC TGTTGTGCCA 
GTACATGTTT TGGATCCAGT TCACTTTTTT GGAAGAATAG TAACTAAAGA GGATGTGTAT 
ACTCATCTAC TTGAAGATCT GAATAAATTT TACCAAATAC CAGGCAATCA AATATTAGTC 
CAGGAAGTAA ACGTTTCACA ATTATATGCT GTACAAGAGG GATCTGTTTA TCACAGGGTT 
AAAGTATTTT CTGCCAAAAA GGAGAATGGA GTTTTGAATT TTAGTTTGCA ATTCATTGAT 
GATGGAAGAA CTGATAAAGT TAGAGATGCC CAACTGTTGC AGTTACCTTC TCAATTTGAA 
AAAATTCCAC CACAGTCAGC TGAGTTTATT GTATGCAGGG TTCAACCAAT TGATAATGAG 
GCTGAATGGG ATCCCATTGT AACAAGGACC ATTAGTAAAG CCATAAAAGG AAAACTTCAT 
ACTGCAAAAG TTGTACTCCA TATTGGGAAT ACATATTGGT TGGATCCAAT GGTGCAAAGA 
AGCAAACTGT CTGGCTTTGC AGCTTGTATA AATGAATTAA ATGTGCGCCA TGAGATCTTG 
TCTACTGGGA TGGGAGTTGA TAATCCTCAG CACATAAGTC ATCTTGAGGA ATTATTGAAA 
AAAACTGGAG TAGCAACTGT GCAGCAAGAC TCTACTGATA AGGTATCAGA TGCAGACTTG 
TCAAGCAATA ATATGATCGC CAATTGCTGT GTTTCTTCTC TTTCCAGTTT GGATGCAAAC 
TGTGGTGCAA GGAATGTGGA CCTACAAAAT AAACTGGAGC GTAATCCACT AGAAAGTAAA 
AGCTTGCAAC CAGAAGTGAA ATGGTTTCAG AAAGATAAAG CAGTAATCCT GACGATAAAG 
TTACATGATG TTACAGATCA TGCCTGTATG TTTTATGCTT CCAGAGTTGT GTTTAGTTGT 
TATGCTGGAG GAAAGCACTA TGTGGCAGAT CTGGAACTTT GTGAGGAGAT ACTAAACGAA 
GAATGTGAGT GCTGCATTAA GAATGGAGAA CCTGTAATAA CATTATTGAA GGCTCAAAAC 
AGACCTTGGA AAAGCCTTCT GAAACAAAAG CATCCAAATG TGAGTCTTGA TTTTGACAAA 
TGGGAAGATT CTGAGGACAG TGATGGTTTT CCTTTTGTCT CCGGAAAGCC CAAGTGTCTT 
TACAAAGTTG TAAGTGAAGA AGTTGAATCT TCGGAATACA GTGAATCTGA CACTGAATCT 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816810.1
CDS	151..3756
Translation	MYEVAVLQIQDPSCFWCEILKSNGQDMKEKVEYEQLYNDLNCLYGKVYRNVEEIKPAFVSVGEFCMVFCEDLNSWCRAILNAPACSAEDNLVECFLVDHAIYCPVKKKNIRLPVEACHRLPFRAKKFKLHQIQPVSLCVDLCGDKAELGVADKWDAAAIEYFKHLLKESVHAEAKLFSIENNILSVYLYIVTTEATVCVNDELVAKSFACFCSDVQSCEKIIPTSLQNMQPSKEVDNPAQMFWPEIYKTKKIPFYEGFPINEKDEMEPRNHCEKKPEEKEMWEENHDFDKLLQIINPEPIKLSENDYIQKPRTSAPQYPIFVRKTIEQCCTLKFTSLTSDLKKELVRNRYCGPSFTESYSWPSVAQGFNTIVISPDGMKPMNYIPPLLTFLHLASAVYTLLPSRNGPLAAILCSGWKKAHFVHELLLKYSKHTRPLNPMLILVGLKEEECENLNIRKGCEVIVTTPPSLLRYLKCKGLLLLRLCHLVLDEVDILFLKSGSQVIEILELFKKSVAAESRECAPQQIVATGTFWHKEMNSLLQYTTEPQVIITRMEQAAVYGNVQQVIQLCLNCEKISVLLRNLDFTPDHAQKVLIFVESDEEAELVHKAVQNHSIFSLVVNRKQVQSFSSVLQQWKNMLSRGTHIVLVVTNEYLPLLEVTDATCIIHFSFPENENVFGLRLYSLLDYIHSKTERVISLEDSFPVARSVLLMTEKHIHHSTDILRYLQHTEATIPPELSDLTQGILQANENRKFDKDICQHIKTFGVCRLNLFTCPDRHNLLPSTDHQYSMTTTNQYISVVPVHVLDPVHFFGRIVTKEDVYTHLLEDLNKFYQIPGNQILVQEVNVSQLYAVQEGSVYHRVKVFSAKKENGVLNFSLQFIDDGRTDKVRDAQLLQLPSQFEKIPPQSAEFIVCRVQPIDNEAEWDPIVTRTISKAIKGKLHTAKVVLHIGNTYWLDPMVQRSKLSGFAACINELNVRHEILSTGMGVDNPQHISHLEELLKKTGVATVQQDSTDKVSDADLSSNNMIANCCVSSLSSLDANCGARNVDLQNKLERNPLESKSLQPEVKWFQKDKAVILTIKLHDVTDHACMFYASRVVFSCYAGGKHYVADLELCEEILNEECECCIKNGEPVITLLKAQNRPWKSLLKQKHPNVSLDFDKWEDSEDSDGFPFVSGKPKCLYKVVSEEVESSEYSESDTESD

Target ORF information:

RefSeq Version	XM_012961356.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tudor domain containing 12 (tdrd12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961356.2

ORF Insert Sequence:

ATGTATGAAG TGGCAGTATT GCAGATTCAG GACCCCAGCT GCTTTTGGTG TGAAATATTA 
AAAAGCAATG GCCAAGATAT GAAGGAGAAA GTGGAATATG AGCAGCTGTA TAATGATCTT 
AATTGTCTTT ATGGAAAGGT CTACAGAAAT GTAGAGGAAA TAAAGCCAGC ATTTGTATCA 
GTTGGAGAGT TTTGCATGGT TTTTTGCGAA GACCTTAATA GTTGGTGCCG AGCTATCCTA 
AATGCACCTG CATGTAGTGC AGAAGATAAC CTTGTGGAGT GCTTCCTAGT GGATCATGCC 
ATTTACTGTC CTGTCAAAAA GAAAAACATT CGCCTTCCTG TGGAAGCTTG TCATCGTCTT 
CCTTTTCGTG CAAAAAAGTT TAAACTTCAC CAAATTCAGC CAGTATCTTT GTGTGTTGAT 
CTTTGTGGAG ATAAAGCAGA ACTTGGAGTT GCTGACAAAT GGGACGCTGC TGCTATTGAG 
TATTTTAAAC ATCTTCTAAA GGAGTCCGTT CATGCTGAAG CAAAATTATT CTCTATAGAA 
AACAATATTT TGAGTGTATA TCTTTACATC GTTACTACAG AGGCTACGGT GTGTGTTAAT 
GATGAACTGG TGGCAAAAAG CTTTGCTTGT TTCTGTTCTG ATGTACAATC TTGTGAAAAA 
ATCATTCCTA CAAGTCTACA AAATATGCAA CCTTCCAAGG AAGTTGATAA TCCTGCACAG 
ATGTTTTGGC CTGAGATCTA TAAAACAAAA AAAATACCAT TTTATGAAGG TTTTCCAATA 
AATGAAAAGG ATGAAATGGA GCCCAGAAAT CACTGTGAAA AGAAACCTGA AGAAAAAGAG 
ATGTGGGAAG AAAACCATGA CTTTGACAAA CTCCTGCAGA TTATCAATCC AGAACCCATA 
AAACTTTCTG AAAATGATTA CATACAGAAA CCAAGAACCA GTGCACCACA ATACCCAATT 
TTTGTGCGCA AAACTATCGA ACAGTGTTGT ACACTTAAAT TTACATCTCT GACGTCTGAT 
CTTAAAAAGG AGCTTGTGAG AAATCGTTAC TGTGGACCAA GTTTCACTGA ATCCTACAGC 
TGGCCTTCTG TAGCACAGGG ATTTAATACC ATTGTAATAT CACCTGATGG CATGAAACCC 
ATGAACTATA TTCCTCCTCT CCTCACATTT TTACACCTTG CTTCAGCAGT GTATACATTA 
CTGCCATCTA GAAATGGGCC TCTTGCAGCC ATTCTTTGCT CTGGATGGAA GAAAGCTCAT 
TTTGTACATG AACTACTTTT AAAGTACAGT AAACACACCA GACCCCTTAA TCCGATGTTG 
ATATTAGTTG GGCTAAAAGA GGAAGAATGC GAAAATCTTA ACATTCGAAA AGGATGTGAG 
GTGATTGTGA CCACACCACC TAGTCTTCTT AGATATCTGA AATGTAAGGG ATTGCTGCTG 
TTAAGGCTTT GTCATCTTGT CTTAGATGAA GTAGACATCC TTTTTTTAAA ATCAGGATCA 
CAGGTAATTG AAATTCTGGA ACTTTTTAAG AAATCAGTTG CAGCAGAAAG CCGAGAATGT 
GCACCTCAGC AGATTGTAGC TACTGGAACA TTTTGGCACA AGGAAATGAA TTCTTTACTG 
CAATATACAA CAGAACCTCA GGTCATAATC ACCAGAATGG AACAGGCTGC AGTGTATGGC 
AATGTCCAGC AGGTGATACA ACTTTGTTTA AACTGTGAAA AAATATCGGT ACTGCTACGG 
AATTTGGATT TCACACCAGA CCATGCTCAA AAGGTCTTGA TATTTGTTGA ATCTGATGAA 
GAGGCTGAGC TGGTTCACAA GGCTGTCCAA AACCATTCCA TTTTCAGTTT AGTTGTAAAC 
AGGAAGCAAG TGCAAAGTTT TAGCAGTGTG TTACAACAAT GGAAAAATAT GTTAAGCCGT 
GGAACACACA TTGTTTTAGT TGTTACAAAT GAATATTTAC CTCTTCTTGA AGTGACAGAT 
GCCACATGTA TAATACACTT CAGTTTTCCA GAAAATGAAA ATGTTTTTGG TCTGCGCCTC 
TATTCCTTAC TGGACTACAT ACACAGTAAA ACAGAGCGGG TAATTTCTTT AGAAGACAGT 
TTCCCAGTAG CCAGATCTGT TTTGCTTATG ACAGAAAAAC ATATACACCA TTCAACTGAT 
ATACTGCGTT ACTTACAACA CACAGAGGCC ACAATTCCGC CAGAGTTAAG TGATCTTACG 
CAAGGAATTT TACAGGCAAA TGAGAATAGG AAGTTTGATA AAGATATTTG TCAGCATATC 
AAAACTTTTG GTGTTTGCAG GTTAAATCTA TTTACATGCC CTGATCGCCA TAACCTCCTT 
CCATCTACAG ACCATCAGTA TTCCATGACA ACAACCAACC AGTACATATC TGTTGTGCCA 
GTACATGTTT TGGATCCAGT TCACTTTTTT GGAAGAATAG TAACTAAAGA GGATGTGTAT 
ACTCATCTAC TTGAAGATCT GAATAAATTT TACCAAATAC CAGGCAATCA AATATTAGTC 
CAGGAAGTAA ACGTTTCACA ATTATATGCT GTACAAGAGG GATCTGTTTA TCACAGGGTT 
AAAGTATTTT CTGCCAAAAA GGAGAATGGA GTTTTGAATT TTAGTTTGCA ATTCATTGAT 
GATGGAAGAA CTGATAAAGT TAGAGATGCC CAACTGTTGC AGTTACCTTC TCAATTTGAA 
AAAATTCCAC CACAGTCAGC TGAGTTTATT GTATGCAGGG TTCAACCAAT TGATAATGAG 
GCTGAATGGG ATCCCATTGT AACAAGGACC ATTAGTAAAG CCATAAAAGG AAAACTTCAT 
ACTGCAAAAG TTGTACTCCA TATTGGGAAT ACATATTGGT TGGATCCAAT GGTGCAAAGA 
AGCAAACTGT CTGGCTTTGC AGCTTGTATA AATGAATTAA ATGTGCGCCA TGAGATCTTG 
TCTACTGGGA TGGGAGTTGA TAATCCTCAG CACATAAGTC ATCTTGAGGA ATTATTGAAA 
AAAACTGGAG TAGCAACTGT GCAGCAAGAC TCTACTGATA AGGTATCAGA TGCAGACTTG 
TCAAGCAATA ATATGATCGC CAATTGCTGT GTTTCTTCTC TTTCCAGTTT GGATGCAAAC 
TGTGGTGCAA GGAATGTGGA CCTACAAAAT AAACTGGAGC GTAATCCACT AGAAAGTAAA 
AGCTTGCAAC CAGAAGTGAA ATGGTTTCAG AAAGATAAAG CAGTAATCCT GACGATAAAG 
TTACATGATG TTACAGATCA TGCCTGTATG TTTTATGCTT CCAGAGTTGT GTTTAGTTGT 
TATGCTGGAG GAAAGCACTA TGTGGCAGAT CTGGAACTTT GTGAGGAGAT ACTAAACGAA 
GAATGTGAGT GCTGCATTAA GAATGGAGAA CCTGTAATAA CATTATTGAA GGCTCAAAAC 
AGACCTTGGA AAAGCCTTCT GAAACAAAAG CATCCAAATG TGAGTCTTGA TTTTGACAAA 
TGGGAAGATT CTGAGGACAG TGATGGTTTT CCTTTTGTCT CCGGAAAGCC CAAGTGTCTT 
TACAAAGTTG TAAGTGAAGA AGTTGAATCT TCGGAATACA GTGAATCTGA CACTGAATCT 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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tdrd12 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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