astn1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following astn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the astn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa56165 XM_012961336.3
Latest version!
Xenopus tropicalis astrotactin 1 (astn1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa23276 NM_001079054.1
Latest version!
Xenopus tropicalis astrotactin 1 (astn1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa29332 XM_012961336.2
Latest version!
Xenopus tropicalis astrotactin 1 (astn1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa56165
    Clone ID Related Accession (Same CDS sequence) XM_012961336.3
    Accession Version XM_012961336.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3909bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product astrotactin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961336.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCTCCTG CCGGTGCTTT CTGGATGCTG TTAACCGGTT ATCTCTTAAT ATCAAGAAGT 
    AGTGGGGAGG AGGACTCGTC CAGAGAAATG GAGTGCAAGA TGAAGAGCAT CACAGTGTCT
    GCTCTCCCCT TTCTCCGGGA AAACGACCTC AGCATCATGC ATAGTCCCTC TGCTTCTGAG
    CCCAAGCTGC TTTTCTCTGT CCGGAATGAC TTCCCAGGGG AAATTGCTGT AGCTGATGAC
    TTGGAGAACA CAGAGCTGCC CTACTTTGTG TTGGAGATTG CAGGGAACAC AGATGATATC
    CCACTTGTCC GCTGGAGGCA GCAGTGGCTT GAAAATGGCA CCCTGCTCTT TCATATACAC
    CACCAAGATG GATCTTCCAG TCTGCCAGGA CAGGAAACTA CAGAAGAACC TCAGGGAGAA
    GCTGCTGAAG AGGAGCTGAG GATACTGCAC ATTTCTGTAA TGGGGGGAAT GATTGCTCTC
    CTTCTCTGCA TTTTGTGTTT ACTGCTGATC TTGTATACAC GGCGTCGCTG GTGCAAGAGA
    AGGCGTGCTC CACAACCACA GAAAAGTGCA AGTGCTGAAG CTGCCAATGA GATCCACTAC
    ATCCCTTCTG TACTCATTGG AGGTGGCCAT GGAAGAGAAA GCCTACGCAA TGCCCGTGGA
    CAATCCACTC ACCCTGGTGG AACACTAAGT ATCCGTGAAA CACCAATCCT GGATGGATAT
    GAATATGATG TCACTGAACT GCGTCATCAC CTTCAGCGGG AATGTATGAA TGGAGGTGAA
    GACTTTAGCA GCCAGGTTTC ACGCACACTG GATTCCCTTC ATGGATGCAA TGAAAAAAAT
    ACCATGGATC TCACACCAGT GTTTGCTTTG AATTCAGCTT CCTCAGGAAG CGACAATGCC
    AAGATGTCTC TCATGAACAA GTACAAAGAC AGCATGATAG CCACCAGCCC AATGGATTCC
    AACCACCATC CCACAACCCT CCTGTCGCAT GGAGCCAGCA TCCAACACAA GCGCATCAAC
    AGCAAGTCAC GAGCGGGCTC AGCATTTCTT AACCCTGAAG GTGACTGTGG GGCAGACCTG
    GACAATGAAA CTCAATTAAC CTTTTATACA GACCCTTCTC GCAGCAGAAG ACGCAGTCGA
    GTGGGCTCAC CACGCAGTCC CGTCAACAAG ATGACTTTGA CTCTGATCAG TGTGACCAGT
    TGTGTCATTG GCTTGGTGTG TTCATCCCAG CTCAGCTGCC CTTTAATTGT GAAGATAACA
    CTTCATGTCC CTGAACATCT CATAGCTGAT GGAAGTCGCT TTATTCTACT GGAAGGTAGC
    CAGCTTGATG CCAGCGATTG GCTTAATCCA GCCCAGGTGG TTCTCTTCTC CCAGATGAAC
    TCCAGTGGTC CCTGGGCCCT AGACCTATGT GCAAGGAGAA TTCTGGATCC TTGTGAACAT
    CTCTGCGACT CTGACACTGG GGAATGCTTG TGCCATGAAG GCTATATGAG GGATCCTGTG
    CACAAACATC TATGTATCCG CAATGAGTGG GGCACTAACC AAGGCCCCTG GCCATATACC
    ATGTTCCAAA GAGGATTTGA TCTAGTACTG GGAGAGCAGC CTTCGGACAA AATCTTCAGA
    TTTACTTATA CATTGGGGGA AGGCATGTGG CTTCCTCTCA GCAAGAGTTT TGTCATACCC
    CCTGCAGAAC TTGCCATTAA TCCATCTGCC AAGTGCAAGA CAGACATGAC TGTTATGGAG
    GATGCTGTGG AAGTCAGAGA GGAGCTCATG ACTTCTTCTT CGTTTGATAA CCTTGAAATC
    CTTCTAGATT CTTTTGGACC TGTCCGGGAT TGCTCCAAGG ATAATGGAGG TTGCACTAAG
    AACTTCCGTT GTGTCTCGGA TCGCAAGCTG GATTCCAGTG GTTGTGTGTG TCCATCAGGA
    CTAAGTCCAA TGAAAGATCT ATCAGGCTGC TATGACCGGC AGATGGGAGT AGATTGTTCT
    GATGGCTTTA ATGGAGGCTG TGAACAGCTC TGTCTCCATG AAGATGTTCC TCTGTCGGAA
    GAGTCAGATT TGTATAACAT TTACATGTAC TGTGGATGTA TAGAGGAATA TAAACTCGGT
    GTGGATGGGC TGGCATGTCA CCTGATAACA GAGCCATGCC CTGGTGGAGA GCACTGTGGT
    GACAACCAGG AGGTGCCTAT GAATCAGACA CTTTTTGGGG AGCTCTTTTA CGGCTACAAT
    AACCTCACCA AGGAACTGGC ACCTAATCAA CTGCTCAAAG GAACTTTCAG GCAAAATAAC
    TTTGCTCGGG GACTAGAACA TCAGCTTCCA GATGGACTCC TTGTAGCGAC CATTCCATCG
    GAAACTCAGT GTCATGAAGA GATATCGGAG CCAGTCCCTG ATCCCGAATT CTTGACAGGT
    ATGGTAAACT TTAGTGAGGT GTCTGGTTAC CCACTAATTC AACACTGGAC TGTTCGATCT
    GTAATTTACC ATGTCCGACT CAACCAACCA TCTCTTTCCC AAGCATTCTC TCAGGCTCTT
    CACTCCCTGG ATGGGGCTAC ATCAAGACTG GAATTTATGG CACTTCTGAA GCAGTTTGGC
    AATCACTATA TCCAAGAAGC AGTTTATGGC TTTGAGGAGT CATGTTCTAT CTGGTATCCT
    AACCGACAAG TGCAAAGACA GCTCTGGCTT GAATTTGAAG ACATCAGCAA AGGTAATACC
    CCTTCAGATG AGTCAGAGGA GCGAGAGAGA GACCCAAAAG TGCTAACATT CCCTGAATAC
    ATCACCAGCC TGCTAGACAC TGGCACAAGG CGGATGGCAC ATGGAGTACG CATGGAGTGC
    CAGAGTCGAG GTCGTTGCTC TTCCTATTGC CCACTGTGCC ATGTAACCTC CAGTCCTGAT
    GCCCCTCCTG AACCAGTTCT TCTGGAAGTC ACAAAGGCAA TTCCAATATA TGAACTGGTC
    ACCAGCAATA ATGCCACTCA GCAGTTGCTG CAGGAAGCTG TACTAAGCTC TATCTGGTGC
    TCTGGCCATG GAGATGTCAT TGAGGACTGG TGCCGCTGTG ACTCAACTGC TTTTGGTGTC
    GACGGGCTTC CTAATTGTGC ACCTTTGCCC CAACCAGTGC TTCGTCTGTC TATGCTGCAG
    GAACCTAGCA GTTCCCTTCT GGTGCTGGAC TGGCTGCACC ATGAATCACC AATAGGGGCC
    CGTATTATAG ATTATCTGAT CAGCCAGGAA AAGGTCACAG ATAGACTGGA CCATTCTAAA
    GTGGAATCAG AGACAGTTTT GAGTTTTGTG GAGGACATTA TCTCAGGAGC AAAGTCACCA
    TGCACAATGC CAGCCCAAGT ACCTGACAAA TATTCTTCCA CTATATCACT CATCATTCGC
    TGCCTGGAGC CTGATACTAC CTACACGTTT ACGCTTTGGG GAGTGGACAA TACAGGAAGG
    CGGTCAGAGC CCAGCCATGT AACAGTTAAA ATGCCCTGCC CAGAGGTGGA TGATGTGAAG
    TCTCAAGAAG TAGCTGACAA GATCTACAAC CTTTTTAATG GGTACACCAG TGGTAAAGAG
    CAGCAGACAG CATATAACAC TCTTTTGGAA CTTGGATCCC CCACGTTGCA CAGAGTTCGC
    TATCATTATA ATCAGCACTT CCAGAGCTTT GGAGAGTTTA CCTGGCGATG TGAAGATGAA
    CTAGGGCCCA GGAAAGCTGG CTTGGTTCTC TCTCAGTTGG ATTCGCTGAG TCCATGGTGC
    AGTCATCTCC TGAGTGGACC TCGGATCACC TTGCGTCGCT CATCTATACA CTACCTGGCT
    TGTCGGTATA GTGAGGTTAA ACCTCATGCA CTCAACTGGC TTGAACTAAG TCGGGATTTG
    CGTAAAAACT GTGAGGAGCA GGTGCTGGTT GTGCTTAGTA ATGACTATGG AGACCGCAAG
    GATAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816790.1
    CDS393..4301
    Translation

    Target ORF information:

    RefSeq Version XM_012961336.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis astrotactin 1 (astn1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961336.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCTCCTG CCGGTGCTTT CTGGATGCTG TTAACCGGTT ATCTCTTAAT ATCAAGAAGT 
    AGTGGGGAGG AGGACTCGTC CAGAGAAATG GAGTGCAAGA TGAAGAGCAT CACAGTGTCT
    GCTCTCCCCT TTCTCCGGGA AAACGACCTC AGCATCATGC ATAGTCCCTC TGCTTCTGAG
    CCCAAGCTGC TTTTCTCTGT CCGGAATGAC TTCCCAGGGG AAATTGCTGT AGCTGATGAC
    TTGGAGAACA CAGAGCTGCC CTACTTTGTG TTGGAGATTG CAGGGAACAC AGATGATATC
    CCACTTGTCC GCTGGAGGCA GCAGTGGCTT GAAAATGGCA CCCTGCTCTT TCATATACAC
    CACCAAGATG GATCTTCCAG TCTGCCAGGA CAGGAAACTA CAGAAGAACC TCAGGGAGAA
    GCTGCTGAAG AGGAGCTGAG GATACTGCAC ATTTCTGTAA TGGGGGGAAT GATTGCTCTC
    CTTCTCTGCA TTTTGTGTTT ACTGCTGATC TTGTATACAC GGCGTCGCTG GTGCAAGAGA
    AGGCGTGCTC CACAACCACA GAAAAGTGCA AGTGCTGAAG CTGCCAATGA GATCCACTAC
    ATCCCTTCTG TACTCATTGG AGGTGGCCAT GGAAGAGAAA GCCTACGCAA TGCCCGTGGA
    CAATCCACTC ACCCTGGTGG AACACTAAGT ATCCGTGAAA CACCAATCCT GGATGGATAT
    GAATATGATG TCACTGAACT GCGTCATCAC CTTCAGCGGG AATGTATGAA TGGAGGTGAA
    GACTTTAGCA GCCAGGTTTC ACGCACACTG GATTCCCTTC ATGGATGCAA TGAAAAAAAT
    ACCATGGATC TCACACCAGT GTTTGCTTTG AATTCAGCTT CCTCAGGAAG CGACAATGCC
    AAGATGTCTC TCATGAACAA GTACAAAGAC AGCATGATAG CCACCAGCCC AATGGATTCC
    AACCACCATC CCACAACCCT CCTGTCGCAT GGAGCCAGCA TCCAACACAA GCGCATCAAC
    AGCAAGTCAC GAGCGGGCTC AGCATTTCTT AACCCTGAAG GTGACTGTGG GGCAGACCTG
    GACAATGAAA CTCAATTAAC CTTTTATACA GACCCTTCTC GCAGCAGAAG ACGCAGTCGA
    GTGGGCTCAC CACGCAGTCC CGTCAACAAG ATGACTTTGA CTCTGATCAG TGTGACCAGT
    TGTGTCATTG GCTTGGTGTG TTCATCCCAG CTCAGCTGCC CTTTAATTGT GAAGATAACA
    CTTCATGTCC CTGAACATCT CATAGCTGAT GGAAGTCGCT TTATTCTACT GGAAGGTAGC
    CAGCTTGATG CCAGCGATTG GCTTAATCCA GCCCAGGTGG TTCTCTTCTC CCAGATGAAC
    TCCAGTGGTC CCTGGGCCCT AGACCTATGT GCAAGGAGAA TTCTGGATCC TTGTGAACAT
    CTCTGCGACT CTGACACTGG GGAATGCTTG TGCCATGAAG GCTATATGAG GGATCCTGTG
    CACAAACATC TATGTATCCG CAATGAGTGG GGCACTAACC AAGGCCCCTG GCCATATACC
    ATGTTCCAAA GAGGATTTGA TCTAGTACTG GGAGAGCAGC CTTCGGACAA AATCTTCAGA
    TTTACTTATA CATTGGGGGA AGGCATGTGG CTTCCTCTCA GCAAGAGTTT TGTCATACCC
    CCTGCAGAAC TTGCCATTAA TCCATCTGCC AAGTGCAAGA CAGACATGAC TGTTATGGAG
    GATGCTGTGG AAGTCAGAGA GGAGCTCATG ACTTCTTCTT CGTTTGATAA CCTTGAAATC
    CTTCTAGATT CTTTTGGACC TGTCCGGGAT TGCTCCAAGG ATAATGGAGG TTGCACTAAG
    AACTTCCGTT GTGTCTCGGA TCGCAAGCTG GATTCCAGTG GTTGTGTGTG TCCATCAGGA
    CTAAGTCCAA TGAAAGATCT ATCAGGCTGC TATGACCGGC AGATGGGAGT AGATTGTTCT
    GATGGCTTTA ATGGAGGCTG TGAACAGCTC TGTCTCCATG AAGATGTTCC TCTGTCGGAA
    GAGTCAGATT TGTATAACAT TTACATGTAC TGTGGATGTA TAGAGGAATA TAAACTCGGT
    GTGGATGGGC TGGCATGTCA CCTGATAACA GAGCCATGCC CTGGTGGAGA GCACTGTGGT
    GACAACCAGG AGGTGCCTAT GAATCAGACA CTTTTTGGGG AGCTCTTTTA CGGCTACAAT
    AACCTCACCA AGGAACTGGC ACCTAATCAA CTGCTCAAAG GAACTTTCAG GCAAAATAAC
    TTTGCTCGGG GACTAGAACA TCAGCTTCCA GATGGACTCC TTGTAGCGAC CATTCCATCG
    GAAACTCAGT GTCATGAAGA GATATCGGAG CCAGTCCCTG ATCCCGAATT CTTGACAGGT
    ATGGTAAACT TTAGTGAGGT GTCTGGTTAC CCACTAATTC AACACTGGAC TGTTCGATCT
    GTAATTTACC ATGTCCGACT CAACCAACCA TCTCTTTCCC AAGCATTCTC TCAGGCTCTT
    CACTCCCTGG ATGGGGCTAC ATCAAGACTG GAATTTATGG CACTTCTGAA GCAGTTTGGC
    AATCACTATA TCCAAGAAGC AGTTTATGGC TTTGAGGAGT CATGTTCTAT CTGGTATCCT
    AACCGACAAG TGCAAAGACA GCTCTGGCTT GAATTTGAAG ACATCAGCAA AGGTAATACC
    CCTTCAGATG AGTCAGAGGA GCGAGAGAGA GACCCAAAAG TGCTAACATT CCCTGAATAC
    ATCACCAGCC TGCTAGACAC TGGCACAAGG CGGATGGCAC ATGGAGTACG CATGGAGTGC
    CAGAGTCGAG GTCGTTGCTC TTCCTATTGC CCACTGTGCC ATGTAACCTC CAGTCCTGAT
    GCCCCTCCTG AACCAGTTCT TCTGGAAGTC ACAAAGGCAA TTCCAATATA TGAACTGGTC
    ACCAGCAATA ATGCCACTCA GCAGTTGCTG CAGGAAGCTG TACTAAGCTC TATCTGGTGC
    TCTGGCCATG GAGATGTCAT TGAGGACTGG TGCCGCTGTG ACTCAACTGC TTTTGGTGTC
    GACGGGCTTC CTAATTGTGC ACCTTTGCCC CAACCAGTGC TTCGTCTGTC TATGCTGCAG
    GAACCTAGCA GTTCCCTTCT GGTGCTGGAC TGGCTGCACC ATGAATCACC AATAGGGGCC
    CGTATTATAG ATTATCTGAT CAGCCAGGAA AAGGTCACAG ATAGACTGGA CCATTCTAAA
    GTGGAATCAG AGACAGTTTT GAGTTTTGTG GAGGACATTA TCTCAGGAGC AAAGTCACCA
    TGCACAATGC CAGCCCAAGT ACCTGACAAA TATTCTTCCA CTATATCACT CATCATTCGC
    TGCCTGGAGC CTGATACTAC CTACACGTTT ACGCTTTGGG GAGTGGACAA TACAGGAAGG
    CGGTCAGAGC CCAGCCATGT AACAGTTAAA ATGCCCTGCC CAGAGGTGGA TGATGTGAAG
    TCTCAAGAAG TAGCTGACAA GATCTACAAC CTTTTTAATG GGTACACCAG TGGTAAAGAG
    CAGCAGACAG CATATAACAC TCTTTTGGAA CTTGGATCCC CCACGTTGCA CAGAGTTCGC
    TATCATTATA ATCAGCACTT CCAGAGCTTT GGAGAGTTTA CCTGGCGATG TGAAGATGAA
    CTAGGGCCCA GGAAAGCTGG CTTGGTTCTC TCTCAGTTGG ATTCGCTGAG TCCATGGTGC
    AGTCATCTCC TGAGTGGACC TCGGATCACC TTGCGTCGCT CATCTATACA CTACCTGGCT
    TGTCGGTATA GTGAGGTTAA ACCTCATGCA CTCAACTGGC TTGAACTAAG TCGGGATTTG
    CGTAAAAACT GTGAGGAGCA GGTGCTGGTT GTGCTTAGTA ATGACTATGG AGACCGCAAG
    GATAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa23276
    Clone ID Related Accession (Same CDS sequence) NM_001079054.1
    Accession Version NM_001079054.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3909bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product astrotactin-1 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC121971.1. ##Evidence-Data-START## Transcript exon combination :: BC121971.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028291, SAMD00028292 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCTCCTG CCGGTGCTTT CTGGATGCTG TTAACCGGTT ATCTCTTAAT ATCAAGAAGT 
    AGTGGGGAGG AGGACTCGTC CAGAGAAATG GAGTGCAAGA TGAAGAGCAT CACAGTGTCT
    GCTCTCCCCT TTCTCCGGGA AAACGACCTC AGCATCATGC ATAGTCCCTC TGCTTCTGAG
    CCCAAGCTGC TTTTCTCTGT CCGGAATGAC TTCCCAGGGG AAATTGCTGT AGCTGATGAC
    TTGGAGAACA CAGAGCTGCC CTACTTTGTG TTGGAGATTG CAGGGAACAC AGATGATATC
    CCACTTGTCC GCTGGAGGCA GCAGTGGCTT GAAAATGGCA CCCTGCTCTT TCATATACAC
    CACCAAGATG GATCTTCCAG TCTGCCAGGA CAGGAAACTA CAGAAGAACC TCAGGGAGAA
    GCTGCTGAAG AGGAGCTGAG GATACTGCAC ATTTCTGTAA TGGGGGGAAT GATTGCTCTC
    CTTCTCTGCA TTTTGTGTTT ACTGCTGATC TTGTATACAC GGCGTCGCTG GTGCAAGAGA
    AGGCGTGCTC CACAACCACA GAAAAGTGCA AGTGCTGAAG CTGCCAATGA GATCCACTAC
    ATCCCTTCTG TACTCATTGG AGGTGGCCAT GGAAGAGAAA GCCTACGCAA TGCCCGTGGA
    CAATCCACTC ACCCTGGTGG AACACTAAGT ATCCGTGAAA CACCAATCCT GGATGGATAT
    GAATATGATG TCACTGAACT GCGTCATCAC CTTCAGCGGG AATGTATGAA TGGAGGTGAA
    GACTTTAGCA GCCAGGTTTC ACGCACACTG GATTCCCTTC ATGGATGCAA TGAAAAAAAT
    ACCATGGATC TCACACCAGT GTTTGCTTTG AATTCAGCTT CCTCAGGAAG CGACAATGCC
    AAGATGTCTC TCATGAACAA GTACAAAGAC AGCATGATAG CCACCAGCCC AATGGATTCC
    AACCACCATC CCACAACCCT CCTGTCGCAT GGAGCCAGCA TCCAACACAA GCGCATCAAC
    AGCAAGTCAC GAGCGGGCTC AGCATTTCTT AACCCTGAAG GTGACTGTGG GGCAGACCTG
    GACAATGAAA CTCAATTAAC CTTTTATACA GACCCTTCTC GCAGCAGAAG ACGCAGTCGA
    GTGGGCTCAC CACGCAGTCC CGTCAACAAG ATGACTTTGA CTCTGATCAG TGTGACCAGT
    TGTGTCATTG GCTTGGTGTG TTCATCCCAG CTCAGCTGCC CTTTAATTGT GAAGATAACA
    CTTCATGTCC CTGAACATCT CATAGCTGAT GGAAGTCGCT TTATTCTACT GGAAGGTAGC
    CAGCTTGATG CCAGCGATTG GCTTAATCCA GCCCAGGTGG TTCTCTTCTC CCAGATGAAC
    TCCAGTGGTC CCTGGGCCCT AGACCTATGT GCAAGGAGAA TTCTGGATCC TTGTGAACAT
    CTCTGCGACT CTGACACTGG GGAATGCTTG TGCCATGAAG GCTATATGAG GGATCCTGTG
    CACAAACATC TATGTATCCG CAATGAGTGG GGCACTAACC AAGGCCCCTG GCCATATACC
    ATGTTCCAAA GAGGATTTGA TCTAGTACTG GGAGAGCAGC CTTCGGACAA AATCTTCAGA
    TTTACTTATA CATTGGGGGA AGGCATGTGG CTTCCTCTCA GCAAGAGTTT TGTCATACCC
    CCTGCAGAAC TTGCCATTAA TCCATCTGCC AAGTGCAAGA CAGACATGAC TGTTATGGAG
    GATGCTGTGG AAGTCAGAGA GGAGCTCATG ACTTCTTCTT CGTTTGATAA CCTTGAAATC
    CTTCTAGATT CTTTTGGACC TGTCCGGGAT TGCTCCAAGG ATAATGGAGG TTGCACTAAG
    AACTTCCGTT GTGTCTCGGA TCGCAAGCTG GATTCCAGTG GTTGTGTGTG TCCATCAGGA
    CTAAGTCCAA TGAAAGATCT ATCAGGCTGC TATGACCGGC AGATGGGAGT AGATTGTTCT
    GATGGCTTTA ATGGAGGCTG TGAACAGCTC TGTCTCCATG AAGATGTTCC TCTGTCGGAA
    GAGTCAGATT TGTATAACAT TTACATGTAC TGTGGATGTA TAGAGGAATA TAAACTCGGT
    GTGGATGGGC TGGCATGTCA CCTGATAACA GAGCCATGCC CTGGTGGAGA GCACTGTGGT
    GACAACCAGG AGGTGCCTAT GAATCAGACA CTTTTTGGGG AGCTCTTTTA CGGCTACAAT
    AACCTCACCA AGGAACTGGC ACCTAATCAA CTGCTCAAAG GAACTTTCAG GCAAAATAAC
    TTTGCTCGGG GACTAGAACA TCAGCTTCCA GATGGACTCC TTGTAGCGAC CATTCCATCG
    GAAACTCAGT GTCATGAAGA GATATCGGAG CCAGTCCCTG ATCCCGAATT CTTGACAGGT
    ATGGTAAACT TTAGTGAGGT GTCTGGTTAC CCACTAATTC AACACTGGAC TGTTCGATCT
    GTAATTTACC ATGTCCGACT CAACCAACCA TCTCTTTCCC AAGCATTCTC TCAGGCTCTT
    CACTCCCTGG ATGGGGCTAC ATCAAGACTG GAATTTATGG CACTTCTGAA GCAGTTTGGC
    AATCACTATA TCCAAGAAGC AGTTTATGGC TTTGAGGAGT CATGTTCTAT CTGGTATCCT
    AACCGACAAG TGCAAAGACA GCTCTGGCTT GAATTTGAAG ACATCAGCAA AGGTAATACC
    CCTTCAGATG AGTCAGAGGA GCGAGAGAGA GACCCAAAAG TGCTAACATT CCCTGAATAC
    ATCACCAGCC TGCTAGACAC TGGCACAAGG CGGATGGCAC ATGGAGTACG CATGGAGTGC
    CAGAGTCGAG GTCGTTGCTC TTCCTATTGC CCACTGTGCC ATGTAACCTC CAGTCCTGAT
    GCCCCTCCTG AACCAGTTCT TCTGGAAGTC ACAAAGGCAA TTCCAATATA TGAACTGGTC
    ACCAGCAATA ATGCCACTCA GCAGTTGCTG CAGGAAGCTG TACTAAGCTC TATCTGGTGC
    TCTGGCCATG GAGATGTCAT TGAGGACTGG TGCCGCTGTG ACTCAACTGC TTTTGGTGTC
    GACGGGCTTC CTAATTGTGC ACCTTTGCCC CAACCAGTGC TTCGTCTGTC TATGCTGCAG
    GAACCTAGCA GTTCCCTTCT GGTGCTGGAC TGGCTGCACC ATGAATCACC AATAGGGGCC
    CGTATTATAG ATTATCTGAT CAGCCAGGAA AAGGTCACAG ATAGACTGGA CCATTCTAAA
    GTGGAATCAG AGACAGTTTT GAGTTTTGTG GAGGACATTA TCTCAGGAGC AAAGTCACCA
    TGCACAATGC CAGCCCAAGT ACCTGACAAA TATTCTTCCA CTATATCACT CATCATTCGC
    TGCCTGGAGC CTGATACTAC CTACACGTTT ACGCTTTGGG GAGTGGACAA TACAGGAAGG
    CGGTCAGAGC CCAGCCACGT AACAGTTAAA ATGCCCTGCC CAGAGGTGGA TGATGTGAAG
    TCTCAAGAAG TAGCTGACAA GATCTACAAC CTTTTTAATG GGTACACCAG TGGTAAAGAG
    CAGCAGACAG CATATAACAC TCTTTTGGAA CTTGGATCCC CCACATTGCA CAGAGTTCGC
    TATCATTATA ATCAGCACTT CCAGAGCTTT GGAGAGTTTA CCTGGCGATG TGAAGATGAA
    CTAGGGCCCA GGAAAGCTGG CTTGGTTCTC TCTCAGTTGG ATTCGCTGAG TCCATGGTGC
    AGTCATCTCC TGAGTGGACC TCGGATCACC TTGCGTCGCT CATCTATACA CTACCTGGCT
    TGTCGGTATA GTGAGGTTAA ACCTCATGCA CTCAACTGGC TTGAACTAAG TCGGGATTTG
    CGTAAAAACT GTGAGGAGCA GGTGCTGGTT GTGCTTAGTA ATGACTATGG AGACCGCAAG
    GATAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001072522.1
    CDS44..3952
    Translation

    Target ORF information:

    RefSeq Version NM_001079054.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis astrotactin 1 (astn1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001079054.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCTCCTG CCGGTGCTTT CTGGATGCTG TTAACCGGTT ATCTCTTAAT ATCAAGAAGT 
    AGTGGGGAGG AGGACTCGTC CAGAGAAATG GAGTGCAAGA TGAAGAGCAT CACAGTGTCT
    GCTCTCCCCT TTCTCCGGGA AAACGACCTC AGCATCATGC ATAGTCCCTC TGCTTCTGAG
    CCCAAGCTGC TTTTCTCTGT CCGGAATGAC TTCCCAGGGG AAATTGCTGT AGCTGATGAC
    TTGGAGAACA CAGAGCTGCC CTACTTTGTG TTGGAGATTG CAGGGAACAC AGATGATATC
    CCACTTGTCC GCTGGAGGCA GCAGTGGCTT GAAAATGGCA CCCTGCTCTT TCATATACAC
    CACCAAGATG GATCTTCCAG TCTGCCAGGA CAGGAAACTA CAGAAGAACC TCAGGGAGAA
    GCTGCTGAAG AGGAGCTGAG GATACTGCAC ATTTCTGTAA TGGGGGGAAT GATTGCTCTC
    CTTCTCTGCA TTTTGTGTTT ACTGCTGATC TTGTATACAC GGCGTCGCTG GTGCAAGAGA
    AGGCGTGCTC CACAACCACA GAAAAGTGCA AGTGCTGAAG CTGCCAATGA GATCCACTAC
    ATCCCTTCTG TACTCATTGG AGGTGGCCAT GGAAGAGAAA GCCTACGCAA TGCCCGTGGA
    CAATCCACTC ACCCTGGTGG AACACTAAGT ATCCGTGAAA CACCAATCCT GGATGGATAT
    GAATATGATG TCACTGAACT GCGTCATCAC CTTCAGCGGG AATGTATGAA TGGAGGTGAA
    GACTTTAGCA GCCAGGTTTC ACGCACACTG GATTCCCTTC ATGGATGCAA TGAAAAAAAT
    ACCATGGATC TCACACCAGT GTTTGCTTTG AATTCAGCTT CCTCAGGAAG CGACAATGCC
    AAGATGTCTC TCATGAACAA GTACAAAGAC AGCATGATAG CCACCAGCCC AATGGATTCC
    AACCACCATC CCACAACCCT CCTGTCGCAT GGAGCCAGCA TCCAACACAA GCGCATCAAC
    AGCAAGTCAC GAGCGGGCTC AGCATTTCTT AACCCTGAAG GTGACTGTGG GGCAGACCTG
    GACAATGAAA CTCAATTAAC CTTTTATACA GACCCTTCTC GCAGCAGAAG ACGCAGTCGA
    GTGGGCTCAC CACGCAGTCC CGTCAACAAG ATGACTTTGA CTCTGATCAG TGTGACCAGT
    TGTGTCATTG GCTTGGTGTG TTCATCCCAG CTCAGCTGCC CTTTAATTGT GAAGATAACA
    CTTCATGTCC CTGAACATCT CATAGCTGAT GGAAGTCGCT TTATTCTACT GGAAGGTAGC
    CAGCTTGATG CCAGCGATTG GCTTAATCCA GCCCAGGTGG TTCTCTTCTC CCAGATGAAC
    TCCAGTGGTC CCTGGGCCCT AGACCTATGT GCAAGGAGAA TTCTGGATCC TTGTGAACAT
    CTCTGCGACT CTGACACTGG GGAATGCTTG TGCCATGAAG GCTATATGAG GGATCCTGTG
    CACAAACATC TATGTATCCG CAATGAGTGG GGCACTAACC AAGGCCCCTG GCCATATACC
    ATGTTCCAAA GAGGATTTGA TCTAGTACTG GGAGAGCAGC CTTCGGACAA AATCTTCAGA
    TTTACTTATA CATTGGGGGA AGGCATGTGG CTTCCTCTCA GCAAGAGTTT TGTCATACCC
    CCTGCAGAAC TTGCCATTAA TCCATCTGCC AAGTGCAAGA CAGACATGAC TGTTATGGAG
    GATGCTGTGG AAGTCAGAGA GGAGCTCATG ACTTCTTCTT CGTTTGATAA CCTTGAAATC
    CTTCTAGATT CTTTTGGACC TGTCCGGGAT TGCTCCAAGG ATAATGGAGG TTGCACTAAG
    AACTTCCGTT GTGTCTCGGA TCGCAAGCTG GATTCCAGTG GTTGTGTGTG TCCATCAGGA
    CTAAGTCCAA TGAAAGATCT ATCAGGCTGC TATGACCGGC AGATGGGAGT AGATTGTTCT
    GATGGCTTTA ATGGAGGCTG TGAACAGCTC TGTCTCCATG AAGATGTTCC TCTGTCGGAA
    GAGTCAGATT TGTATAACAT TTACATGTAC TGTGGATGTA TAGAGGAATA TAAACTCGGT
    GTGGATGGGC TGGCATGTCA CCTGATAACA GAGCCATGCC CTGGTGGAGA GCACTGTGGT
    GACAACCAGG AGGTGCCTAT GAATCAGACA CTTTTTGGGG AGCTCTTTTA CGGCTACAAT
    AACCTCACCA AGGAACTGGC ACCTAATCAA CTGCTCAAAG GAACTTTCAG GCAAAATAAC
    TTTGCTCGGG GACTAGAACA TCAGCTTCCA GATGGACTCC TTGTAGCGAC CATTCCATCG
    GAAACTCAGT GTCATGAAGA GATATCGGAG CCAGTCCCTG ATCCCGAATT CTTGACAGGT
    ATGGTAAACT TTAGTGAGGT GTCTGGTTAC CCACTAATTC AACACTGGAC TGTTCGATCT
    GTAATTTACC ATGTCCGACT CAACCAACCA TCTCTTTCCC AAGCATTCTC TCAGGCTCTT
    CACTCCCTGG ATGGGGCTAC ATCAAGACTG GAATTTATGG CACTTCTGAA GCAGTTTGGC
    AATCACTATA TCCAAGAAGC AGTTTATGGC TTTGAGGAGT CATGTTCTAT CTGGTATCCT
    AACCGACAAG TGCAAAGACA GCTCTGGCTT GAATTTGAAG ACATCAGCAA AGGTAATACC
    CCTTCAGATG AGTCAGAGGA GCGAGAGAGA GACCCAAAAG TGCTAACATT CCCTGAATAC
    ATCACCAGCC TGCTAGACAC TGGCACAAGG CGGATGGCAC ATGGAGTACG CATGGAGTGC
    CAGAGTCGAG GTCGTTGCTC TTCCTATTGC CCACTGTGCC ATGTAACCTC CAGTCCTGAT
    GCCCCTCCTG AACCAGTTCT TCTGGAAGTC ACAAAGGCAA TTCCAATATA TGAACTGGTC
    ACCAGCAATA ATGCCACTCA GCAGTTGCTG CAGGAAGCTG TACTAAGCTC TATCTGGTGC
    TCTGGCCATG GAGATGTCAT TGAGGACTGG TGCCGCTGTG ACTCAACTGC TTTTGGTGTC
    GACGGGCTTC CTAATTGTGC ACCTTTGCCC CAACCAGTGC TTCGTCTGTC TATGCTGCAG
    GAACCTAGCA GTTCCCTTCT GGTGCTGGAC TGGCTGCACC ATGAATCACC AATAGGGGCC
    CGTATTATAG ATTATCTGAT CAGCCAGGAA AAGGTCACAG ATAGACTGGA CCATTCTAAA
    GTGGAATCAG AGACAGTTTT GAGTTTTGTG GAGGACATTA TCTCAGGAGC AAAGTCACCA
    TGCACAATGC CAGCCCAAGT ACCTGACAAA TATTCTTCCA CTATATCACT CATCATTCGC
    TGCCTGGAGC CTGATACTAC CTACACGTTT ACGCTTTGGG GAGTGGACAA TACAGGAAGG
    CGGTCAGAGC CCAGCCACGT AACAGTTAAA ATGCCCTGCC CAGAGGTGGA TGATGTGAAG
    TCTCAAGAAG TAGCTGACAA GATCTACAAC CTTTTTAATG GGTACACCAG TGGTAAAGAG
    CAGCAGACAG CATATAACAC TCTTTTGGAA CTTGGATCCC CCACATTGCA CAGAGTTCGC
    TATCATTATA ATCAGCACTT CCAGAGCTTT GGAGAGTTTA CCTGGCGATG TGAAGATGAA
    CTAGGGCCCA GGAAAGCTGG CTTGGTTCTC TCTCAGTTGG ATTCGCTGAG TCCATGGTGC
    AGTCATCTCC TGAGTGGACC TCGGATCACC TTGCGTCGCT CATCTATACA CTACCTGGCT
    TGTCGGTATA GTGAGGTTAA ACCTCATGCA CTCAACTGGC TTGAACTAAG TCGGGATTTG
    CGTAAAAACT GTGAGGAGCA GGTGCTGGTT GTGCTTAGTA ATGACTATGG AGACCGCAAG
    GATAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29332
    Clone ID Related Accession (Same CDS sequence) XM_012961336.2
    Accession Version XM_012961336.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3909bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product astrotactin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961336.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCTCCTG CCGGTGCTTT CTGGATGCTG TTAACCGGTT ATCTCTTAAT ATCAAGAAGT 
    AGTGGGGAGG AGGACTCGTC CAGAGAAATG GAGTGCAAGA TGAAGAGCAT CACAGTGTCT
    GCTCTCCCCT TTCTCCGGGA AAACGACCTC AGCATCATGC ATAGTCCCTC TGCTTCTGAG
    CCCAAGCTGC TTTTCTCTGT CCGGAATGAC TTCCCAGGGG AAATTGCTGT AGCTGATGAC
    TTGGAGAACA CAGAGCTGCC CTACTTTGTG TTGGAGATTG CAGGGAACAC AGATGATATC
    CCACTTGTCC GCTGGAGGCA GCAGTGGCTT GAAAATGGCA CCCTGCTCTT TCATATACAC
    CACCAAGATG GATCTTCCAG TCTGCCAGGA CAGGAAACTA CAGAAGAACC TCAGGGAGAA
    GCTGCTGAAG AGGAGCTGAG GATACTGCAC ATTTCTGTAA TGGGGGGAAT GATTGCTCTC
    CTTCTCTGCA TTTTGTGTTT ACTGCTGATC TTGTATACAC GGCGTCGCTG GTGCAAGAGA
    AGGCGTGCTC CACAACCACA GAAAAGTGCA AGTGCTGAAG CTGCCAATGA GATCCACTAC
    ATCCCTTCTG TACTCATTGG AGGTGGCCAT GGAAGAGAAA GCCTACGCAA TGCCCGTGGA
    CAATCCACTC ACCCTGGTGG AACACTAAGT ATCCGTGAAA CACCAATCCT GGATGGATAT
    GAATATGATG TCACTGAACT GCGTCATCAC CTTCAGCGGG AATGTATGAA TGGAGGTGAA
    GACTTTAGCA GCCAGGTTTC ACGCACACTG GATTCCCTTC ATGGATGCAA TGAAAAAAAT
    ACCATGGATC TCACACCAGT GTTTGCTTTG AATTCAGCTT CCTCAGGAAG CGACAATGCC
    AAGATGTCTC TCATGAACAA GTACAAAGAC AGCATGATAG CCACCAGCCC AATGGATTCC
    AACCACCATC CCACAACCCT CCTGTCGCAT GGAGCCAGCA TCCAACACAA GCGCATCAAC
    AGCAAGTCAC GAGCGGGCTC AGCATTTCTT AACCCTGAAG GTGACTGTGG GGCAGACCTG
    GACAATGAAA CTCAATTAAC CTTTTATACA GACCCTTCTC GCAGCAGAAG ACGCAGTCGA
    GTGGGCTCAC CACGCAGTCC CGTCAACAAG ATGACTTTGA CTCTGATCAG TGTGACCAGT
    TGTGTCATTG GCTTGGTGTG TTCATCCCAG CTCAGCTGCC CTTTAATTGT GAAGATAACA
    CTTCATGTCC CTGAACATCT CATAGCTGAT GGAAGTCGCT TTATTCTACT GGAAGGTAGC
    CAGCTTGATG CCAGCGATTG GCTTAATCCA GCCCAGGTGG TTCTCTTCTC CCAGATGAAC
    TCCAGTGGTC CCTGGGCCCT AGACCTATGT GCAAGGAGAA TTCTGGATCC TTGTGAACAT
    CTCTGCGACT CTGACACTGG GGAATGCTTG TGCCATGAAG GCTATATGAG GGATCCTGTG
    CACAAACATC TATGTATCCG CAATGAGTGG GGCACTAACC AAGGCCCCTG GCCATATACC
    ATGTTCCAAA GAGGATTTGA TCTAGTACTG GGAGAGCAGC CTTCGGACAA AATCTTCAGA
    TTTACTTATA CATTGGGGGA AGGCATGTGG CTTCCTCTCA GCAAGAGTTT TGTCATACCC
    CCTGCAGAAC TTGCCATTAA TCCATCTGCC AAGTGCAAGA CAGACATGAC TGTTATGGAG
    GATGCTGTGG AAGTCAGAGA GGAGCTCATG ACTTCTTCTT CGTTTGATAA CCTTGAAATC
    CTTCTAGATT CTTTTGGACC TGTCCGGGAT TGCTCCAAGG ATAATGGAGG TTGCACTAAG
    AACTTCCGTT GTGTCTCGGA TCGCAAGCTG GATTCCAGTG GTTGTGTGTG TCCATCAGGA
    CTAAGTCCAA TGAAAGATCT ATCAGGCTGC TATGACCGGC AGATGGGAGT AGATTGTTCT
    GATGGCTTTA ATGGAGGCTG TGAACAGCTC TGTCTCCATG AAGATGTTCC TCTGTCGGAA
    GAGTCAGATT TGTATAACAT TTACATGTAC TGTGGATGTA TAGAGGAATA TAAACTCGGT
    GTGGATGGGC TGGCATGTCA CCTGATAACA GAGCCATGCC CTGGTGGAGA GCACTGTGGT
    GACAACCAGG AGGTGCCTAT GAATCAGACA CTTTTTGGGG AGCTCTTTTA CGGCTACAAT
    AACCTCACCA AGGAACTGGC ACCTAATCAA CTGCTCAAAG GAACTTTCAG GCAAAATAAC
    TTTGCTCGGG GACTAGAACA TCAGCTTCCA GATGGACTCC TTGTAGCGAC CATTCCATCG
    GAAACTCAGT GTCATGAAGA GATATCGGAG CCAGTCCCTG ATCCCGAATT CTTGACAGGT
    ATGGTAAACT TTAGTGAGGT GTCTGGTTAC CCACTAATTC AACACTGGAC TGTTCGATCT
    GTAATTTACC ATGTCCGACT CAACCAACCA TCTCTTTCCC AAGCATTCTC TCAGGCTCTT
    CACTCCCTGG ATGGGGCTAC ATCAAGACTG GAATTTATGG CACTTCTGAA GCAGTTTGGC
    AATCACTATA TCCAAGAAGC AGTTTATGGC TTTGAGGAGT CATGTTCTAT CTGGTATCCT
    AACCGACAAG TGCAAAGACA GCTCTGGCTT GAATTTGAAG ACATCAGCAA AGGTAATACC
    CCTTCAGATG AGTCAGAGGA GCGAGAGAGA GACCCAAAAG TGCTAACATT CCCTGAATAC
    ATCACCAGCC TGCTAGACAC TGGCACAAGG CGGATGGCAC ATGGAGTACG CATGGAGTGC
    CAGAGTCGAG GTCGTTGCTC TTCCTATTGC CCACTGTGCC ATGTAACCTC CAGTCCTGAT
    GCCCCTCCTG AACCAGTTCT TCTGGAAGTC ACAAAGGCAA TTCCAATATA TGAACTGGTC
    ACCAGCAATA ATGCCACTCA GCAGTTGCTG CAGGAAGCTG TACTAAGCTC TATCTGGTGC
    TCTGGCCATG GAGATGTCAT TGAGGACTGG TGCCGCTGTG ACTCAACTGC TTTTGGTGTC
    GACGGGCTTC CTAATTGTGC ACCTTTGCCC CAACCAGTGC TTCGTCTGTC TATGCTGCAG
    GAACCTAGCA GTTCCCTTCT GGTGCTGGAC TGGCTGCACC ATGAATCACC AATAGGGGCC
    CGTATTATAG ATTATCTGAT CAGCCAGGAA AAGGTCACAG ATAGACTGGA CCATTCTAAA
    GTGGAATCAG AGACAGTTTT GAGTTTTGTG GAGGACATTA TCTCAGGAGC AAAGTCACCA
    TGCACAATGC CAGCCCAAGT ACCTGACAAA TATTCTTCCA CTATCTCACT CATCATTCGC
    TGCCTGGAGC CTGATACTAC CTACACGTTT ACGCTTTGGG GAGTGGACAA TACAGGAAGG
    CGGTCAGAGC CCAGCCATGT AACAGTTAAA ATGCCCTGCC CAGAGGTGGA TGATGTGAAG
    TCTCAAGAAG TAGCTGACAA GATCTACAAC CTTTTTAATG GGTACACCAG TGGTAAAGAG
    CAGCAGACAG CATATAACAC TCTTTTGGAA CTTGGATCCC CCACATTGCA CAGAGTTCGC
    TATCATTATA ATCAGCACTT CCAGAGCTTT GGAGAGTTTA CCTGGCGATG TGAAGATGAA
    CTAGGGCCCA GGAAAGCTGG CTTGGTTCTC TCTCAGTTGG ATTCGCTGAG TCCATGGTGC
    AGTCATCTCC TGAGTGGACC TCGGATCACC TTGCGTCGCT CTTCTATACA CTACCTGGCT
    TGTCGGTATA GTGAGGTTAA ACCTCATGCA CTCAACTGGC TTGAACTAAG TCGGGATTTG
    CGTAAAAACT GTGAGGAGCA GGTGCTGGTT GTGCTTAGTA ATGACTATGG AGACCGCAAG
    GATAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816790.1
    CDS246..4154
    Translation

    Target ORF information:

    RefSeq Version XM_012961336.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis astrotactin 1 (astn1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961336.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCTCCTG CCGGTGCTTT CTGGATGCTG TTAACCGGTT ATCTCTTAAT ATCAAGAAGT 
    AGTGGGGAGG AGGACTCGTC CAGAGAAATG GAGTGCAAGA TGAAGAGCAT CACAGTGTCT
    GCTCTCCCCT TTCTCCGGGA AAACGACCTC AGCATCATGC ATAGTCCCTC TGCTTCTGAG
    CCCAAGCTGC TTTTCTCTGT CCGGAATGAC TTCCCAGGGG AAATTGCTGT AGCTGATGAC
    TTGGAGAACA CAGAGCTGCC CTACTTTGTG TTGGAGATTG CAGGGAACAC AGATGATATC
    CCACTTGTCC GCTGGAGGCA GCAGTGGCTT GAAAATGGCA CCCTGCTCTT TCATATACAC
    CACCAAGATG GATCTTCCAG TCTGCCAGGA CAGGAAACTA CAGAAGAACC TCAGGGAGAA
    GCTGCTGAAG AGGAGCTGAG GATACTGCAC ATTTCTGTAA TGGGGGGAAT GATTGCTCTC
    CTTCTCTGCA TTTTGTGTTT ACTGCTGATC TTGTATACAC GGCGTCGCTG GTGCAAGAGA
    AGGCGTGCTC CACAACCACA GAAAAGTGCA AGTGCTGAAG CTGCCAATGA GATCCACTAC
    ATCCCTTCTG TACTCATTGG AGGTGGCCAT GGAAGAGAAA GCCTACGCAA TGCCCGTGGA
    CAATCCACTC ACCCTGGTGG AACACTAAGT ATCCGTGAAA CACCAATCCT GGATGGATAT
    GAATATGATG TCACTGAACT GCGTCATCAC CTTCAGCGGG AATGTATGAA TGGAGGTGAA
    GACTTTAGCA GCCAGGTTTC ACGCACACTG GATTCCCTTC ATGGATGCAA TGAAAAAAAT
    ACCATGGATC TCACACCAGT GTTTGCTTTG AATTCAGCTT CCTCAGGAAG CGACAATGCC
    AAGATGTCTC TCATGAACAA GTACAAAGAC AGCATGATAG CCACCAGCCC AATGGATTCC
    AACCACCATC CCACAACCCT CCTGTCGCAT GGAGCCAGCA TCCAACACAA GCGCATCAAC
    AGCAAGTCAC GAGCGGGCTC AGCATTTCTT AACCCTGAAG GTGACTGTGG GGCAGACCTG
    GACAATGAAA CTCAATTAAC CTTTTATACA GACCCTTCTC GCAGCAGAAG ACGCAGTCGA
    GTGGGCTCAC CACGCAGTCC CGTCAACAAG ATGACTTTGA CTCTGATCAG TGTGACCAGT
    TGTGTCATTG GCTTGGTGTG TTCATCCCAG CTCAGCTGCC CTTTAATTGT GAAGATAACA
    CTTCATGTCC CTGAACATCT CATAGCTGAT GGAAGTCGCT TTATTCTACT GGAAGGTAGC
    CAGCTTGATG CCAGCGATTG GCTTAATCCA GCCCAGGTGG TTCTCTTCTC CCAGATGAAC
    TCCAGTGGTC CCTGGGCCCT AGACCTATGT GCAAGGAGAA TTCTGGATCC TTGTGAACAT
    CTCTGCGACT CTGACACTGG GGAATGCTTG TGCCATGAAG GCTATATGAG GGATCCTGTG
    CACAAACATC TATGTATCCG CAATGAGTGG GGCACTAACC AAGGCCCCTG GCCATATACC
    ATGTTCCAAA GAGGATTTGA TCTAGTACTG GGAGAGCAGC CTTCGGACAA AATCTTCAGA
    TTTACTTATA CATTGGGGGA AGGCATGTGG CTTCCTCTCA GCAAGAGTTT TGTCATACCC
    CCTGCAGAAC TTGCCATTAA TCCATCTGCC AAGTGCAAGA CAGACATGAC TGTTATGGAG
    GATGCTGTGG AAGTCAGAGA GGAGCTCATG ACTTCTTCTT CGTTTGATAA CCTTGAAATC
    CTTCTAGATT CTTTTGGACC TGTCCGGGAT TGCTCCAAGG ATAATGGAGG TTGCACTAAG
    AACTTCCGTT GTGTCTCGGA TCGCAAGCTG GATTCCAGTG GTTGTGTGTG TCCATCAGGA
    CTAAGTCCAA TGAAAGATCT ATCAGGCTGC TATGACCGGC AGATGGGAGT AGATTGTTCT
    GATGGCTTTA ATGGAGGCTG TGAACAGCTC TGTCTCCATG AAGATGTTCC TCTGTCGGAA
    GAGTCAGATT TGTATAACAT TTACATGTAC TGTGGATGTA TAGAGGAATA TAAACTCGGT
    GTGGATGGGC TGGCATGTCA CCTGATAACA GAGCCATGCC CTGGTGGAGA GCACTGTGGT
    GACAACCAGG AGGTGCCTAT GAATCAGACA CTTTTTGGGG AGCTCTTTTA CGGCTACAAT
    AACCTCACCA AGGAACTGGC ACCTAATCAA CTGCTCAAAG GAACTTTCAG GCAAAATAAC
    TTTGCTCGGG GACTAGAACA TCAGCTTCCA GATGGACTCC TTGTAGCGAC CATTCCATCG
    GAAACTCAGT GTCATGAAGA GATATCGGAG CCAGTCCCTG ATCCCGAATT CTTGACAGGT
    ATGGTAAACT TTAGTGAGGT GTCTGGTTAC CCACTAATTC AACACTGGAC TGTTCGATCT
    GTAATTTACC ATGTCCGACT CAACCAACCA TCTCTTTCCC AAGCATTCTC TCAGGCTCTT
    CACTCCCTGG ATGGGGCTAC ATCAAGACTG GAATTTATGG CACTTCTGAA GCAGTTTGGC
    AATCACTATA TCCAAGAAGC AGTTTATGGC TTTGAGGAGT CATGTTCTAT CTGGTATCCT
    AACCGACAAG TGCAAAGACA GCTCTGGCTT GAATTTGAAG ACATCAGCAA AGGTAATACC
    CCTTCAGATG AGTCAGAGGA GCGAGAGAGA GACCCAAAAG TGCTAACATT CCCTGAATAC
    ATCACCAGCC TGCTAGACAC TGGCACAAGG CGGATGGCAC ATGGAGTACG CATGGAGTGC
    CAGAGTCGAG GTCGTTGCTC TTCCTATTGC CCACTGTGCC ATGTAACCTC CAGTCCTGAT
    GCCCCTCCTG AACCAGTTCT TCTGGAAGTC ACAAAGGCAA TTCCAATATA TGAACTGGTC
    ACCAGCAATA ATGCCACTCA GCAGTTGCTG CAGGAAGCTG TACTAAGCTC TATCTGGTGC
    TCTGGCCATG GAGATGTCAT TGAGGACTGG TGCCGCTGTG ACTCAACTGC TTTTGGTGTC
    GACGGGCTTC CTAATTGTGC ACCTTTGCCC CAACCAGTGC TTCGTCTGTC TATGCTGCAG
    GAACCTAGCA GTTCCCTTCT GGTGCTGGAC TGGCTGCACC ATGAATCACC AATAGGGGCC
    CGTATTATAG ATTATCTGAT CAGCCAGGAA AAGGTCACAG ATAGACTGGA CCATTCTAAA
    GTGGAATCAG AGACAGTTTT GAGTTTTGTG GAGGACATTA TCTCAGGAGC AAAGTCACCA
    TGCACAATGC CAGCCCAAGT ACCTGACAAA TATTCTTCCA CTATCTCACT CATCATTCGC
    TGCCTGGAGC CTGATACTAC CTACACGTTT ACGCTTTGGG GAGTGGACAA TACAGGAAGG
    CGGTCAGAGC CCAGCCATGT AACAGTTAAA ATGCCCTGCC CAGAGGTGGA TGATGTGAAG
    TCTCAAGAAG TAGCTGACAA GATCTACAAC CTTTTTAATG GGTACACCAG TGGTAAAGAG
    CAGCAGACAG CATATAACAC TCTTTTGGAA CTTGGATCCC CCACATTGCA CAGAGTTCGC
    TATCATTATA ATCAGCACTT CCAGAGCTTT GGAGAGTTTA CCTGGCGATG TGAAGATGAA
    CTAGGGCCCA GGAAAGCTGG CTTGGTTCTC TCTCAGTTGG ATTCGCTGAG TCCATGGTGC
    AGTCATCTCC TGAGTGGACC TCGGATCACC TTGCGTCGCT CTTCTATACA CTACCTGGCT
    TGTCGGTATA GTGAGGTTAA ACCTCATGCA CTCAACTGGC TTGAACTAAG TCGGGATTTG
    CGTAAAAACT GTGAGGAGCA GGTGCTGGTT GTGCTTAGTA ATGACTATGG AGACCGCAAG
    GATAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.