braf cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	braf
Entrez Gene ID	779570
Full Name	B-Raf proto-oncogene, serine/threonine kinase
Synonyms	B-Raf,b-raf1,braf1,raf,rafb1
General protein information	Preferred Names B-Raf proto-oncogene, serine/threonine kinase Names serine/threonine-protein kinase B-raf B-Raf proto-oncogene serine/threonine-protein kinase b-raf 1 v-raf murine sarcoma viral oncogene homolog B v-raf murine sarcoma viral oncogene homolog B1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031898530.1	XP_031754390.1	serine/threonine-protein kinase B-raf isoform X3
XM_031898532.1	XP_031754392.1	serine/threonine-protein kinase B-raf isoform X5
XM_031898529.1	XP_031754389.1	serine/threonine-protein kinase B-raf isoform X2
XM_031898531.1	XP_031754391.1	serine/threonine-protein kinase B-raf isoform X4
XM_031898533.1	XP_031754393.1	serine/threonine-protein kinase B-raf isoform X6
XM_012959338.2	XP_012814792.1	serine/threonine-protein kinase B-raf
XM_031898528.1	XP_031754388.1	serine/threonine-protein kinase B-raf isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Braf	NP_647455.3
Rattus norvegicus (Norway rat)	Braf	XP_006236418.1
Gallus gallus (chicken)	BRAF	NP_990633.1
Caenorhabditis elegans (roundworm)	lin-45	NP_741430.3
Pan troglodytes (chimpanzee)	BRAF	XP_003951208.1
	LOC693554	XP_002803541.1
Bos taurus (cattle)	BRAF	XP_005205968.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004699	XP_318144.4
Canis lupus familiaris (dog)	BRAF	XP_005629607.1
Drosophila melanogaster (fruit fly)	phl	NP_525047.1
Xenopus tropicalis (tropical clawed frog)	braf	XP_004912520.1
Danio rerio (zebrafish)	braf	NP_991307.2
Homo sapiens (human)	BRAF	NP_004324.2

Gene Ontology
Bibliography

Related articles in PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA,
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following braf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the braf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa28993	XM_012959338.2 Latest version!	Xenopus tropicalis B-Raf proto-oncogene, serine/threonine kinase (braf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa54165	XM_031898528.1 Latest version!	Xenopus tropicalis B-Raf proto-oncogene, serine/threonine kinase (braf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa54166	XM_031898529.1 Latest version!	Xenopus tropicalis B-Raf proto-oncogene, serine/threonine kinase (braf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa54167	XM_031898530.1 Latest version!	Xenopus tropicalis B-Raf proto-oncogene, serine/threonine kinase (braf), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa54168	XM_031898531.1 Latest version!	Xenopus tropicalis B-Raf proto-oncogene, serine/threonine kinase (braf), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa54169	XM_031898532.1 Latest version!	Xenopus tropicalis B-Raf proto-oncogene, serine/threonine kinase (braf), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa54170	XM_031898533.1 Latest version!	Xenopus tropicalis B-Raf proto-oncogene, serine/threonine kinase (braf), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

braf ( XM_012959338.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814792.1 Xenopus tropicalis B-Raf proto-oncogene, serine/threonine kinase (braf), mRNA.

Price & Availability↑

CloneID	OXa28993
Clone ID Related Accession (Same CDS sequence)	XM_012959338.2
Accession Version	XM_012959338.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase B-raf
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683803.1) and transcript sequence (BC121876.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959338.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 103 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTCGCTC CCGCGCCTCC GGACCACTTC AAGATGGCGG CGGTAAGTGG GGGATCAACC 
GAGGGGCCCT CACTGCTCAA CGGGGACCTG CTGGAGTCCG AGACCGGGGG ACTGGGCGGA 
GCCGGCGAGA TGTGTGCGTC CGGGGGGTAC CCGGAGGAGG TCTGGAATAT AAAGCAGATG 
ATAAAGTTAA CACAAGAGCA CATCGAAGCC CTGCTGGACA AATTTGGAGG CGAGCACAAC 
CCCCCATCTA TATACGTAGA GGCTTATGAG GAATACACCA GCAAACTGGA TCTATTACAG 
CAGAGGGAAC AGCAGTTACT GGAGTCTATG GGCAATGGAA CAGACTCTAC TTCTTTGCCT 
TCAGAGCTGA CACTTTCCTC GGTCCCATCT AATCTATCCT TGGCTCCTTC AGCTCTCTCA 
GCCCTGCGGA CCCCCAGCGA CCCCTCCCGT ATCAATCCCA AGTCTCCCCA GAAACCTATT 
GTCCGAGTCT TCCTTCCCAA CAAGCAGCGC ACTGTGGTAC CAGCTAGGAG TGGTGTGACA 
GTGCGAGACA GCCTAAAGAA AGCCCTGATG ATGCGAGGGT TAATCCCAGA ATGCTGTGCT 
GTGTACAGGG TGCAAGATGG GGAGAAGAAG CCGATTGGCT GGGATACAGA TATAAGCTGG 
ATCACTGGAG AAGAGTTACA CGTGGAAGTC TTGGAGAATG TGCCGCTCAC AACCCATAAC 
TTTGTACGGA AAACCTTCTT CACGCTGGCT TTCTGCGACT TCTGTCGGAA GCTGCTGTTC 
CAGGGCTTCC GCTGCCAGAC GTGCGGGTAC AAGTTCCACC AGCGGTGCAG CACGGAGGTG 
CCTCTTATGT GTGTTAACTA CGACCGTCTA GATTTGCTGT TTGTCTCCAA GTTCTTTGAA 
CACCACCCAA TATCTCAGGA GGAAGCTCCG TTTGAAGAGG TCGCCCCAGC CGCTGAGTCC 
TCGACTCCTC CGCCCCCTCC AGATGCCACA GGCCCCCTCT GTCTACCGAG TACTTCTCCT 
TCCAAATCCA TCCCGATTCC CCAGCCGTTC CGACCGGATG AGGACCACAG GAACCAGTTT 
GGCCAGCGAG ATCGGTCGTC CTCGGCGCCC AACGTGCACA TAAATACCAT CGAGCCGGTC 
AATATTGACG ACTTGATTCG AGACCAGGGC CTCCAGTGCA GCGAGGGAGC CCCCTCAGTC 
CAGCCGACGC GCTGCCATCG GAAATACCAC TCGCGGGACC CCAGCCCACT CCTCCATACT 
CTCCCCAGTG AAATAGTCTT TGATTTTGAA TCTGATCCTG TTTGCAGAGG TTCAACCCCC 
GGCTTATCGG CGACCCCCCC TGCCTCCCTC CCCGGCTCCC TCACCAACGT CAAGGCGCTA 
CAGAAATCCC CGGGGCAGCA GAGAGAGCGC AAGTCCTCCT CGTCCTCGAC GGAAGACAGG 
AGTCGGATGA AAACGCTCGG ACGACGCGAT TCCAGCGACG ACTGGGAGAT TCCCGACGGG 
CAAATCACGG TTGGGCAGAG AATAGGTTCT GGATCGTTTG GGACAGTGTA TAAAGGAAAG 
TGGCACGGGG ACGTAGCAGT GAAAATGTTG AATGTAACGG CGCCAACGCC TCAGCAGCTA 
CAGGCCTTTA AAAATGAAGT AGGAGTCCTC AGAAAAACGC GACACGTGAA CATCCTGCTT 
TTCATGGGCT ACTCCACGAA GCCCCAGCTG CGACTAGTGA CCCAGTGGTG CGAGGGCTCC 
AGTCTCTATC ACCACCTGCA CATCATCGAG ACCAAATTCG AAATGATCAA GCTGATCGAC 
ATTGCACGAC AGACGGCGCA GGGCATGGAC TATTTGCACG CGAAGTCAAT CATCCACAGA 
GACCTAAAGA GCAATAATAT ATTCCTTCAT GAAGACCTCA CAGTAAAGAT TGGTGACTTT 
GGCCTGGCAA CAGTGAAGTC CCGGTGGAGT GGGTCTCATC AGTTTGAGCA GTTGTCTGGC 
TCCATTCTGT GGATGGCTCC TGAGGTCATT CGGATGCAAG ATAACAACCC CTATAGCTTT 
CAGTCTGATG TCTACGCGTT TGGCATTGTT CTATATGAAC TGATGACGGG GCAGTTGCCC 
TACTCCAACA TCAACAACAG AGACCAGATC ATATTTATGG TTGGGAGAGG CTACTTATCT 
CCCGACTTCA GCAAAGTTAG GAGTAATTGC CCCAAAGCAA TGAAGAGGTT AATGGCAGAC 
TGTCTAAAGA AGAAGAGAGA TGAGCGCCCC CTATTCCCAC AGATTCTCGC CTCCATCGAG 
CTGCTGGCAC GGTCATTGCC AAAAATCCAC CGCAGCGCGT CTGAGCCGTC CTTAAACCGC 
GCTGGATTCC AGACGGAAGA CTTCAGTCTG TACACGTGCG CTTCTCCCAA AACGCCTATC 
CAGGCGGGAG GTTATGGAAT CTCACCAAGA TGGGTAAATA ACTTTAATGA ACCTCTTTTT 
TATCGGTGTC CAAATCAGCA GAGTATCAAA GAAATCACAA GTATGCGTGA CAGTCAGAGG 
TGGGACCGAC TATGGGATTT TGTCTGTGAG AGATTATTCA ACTCCGTTCC TACTTGTGAA 
TGGAGCAACT ATGTCAATAA CATTGGCCAA GGATTTACCT TCTATTGCCC AACTGGCCAG 
GTTCTCAGTG GAATGGGAAA TGAACTTGAT GCCTGGGAGT CAGACAGGAG GTGGAAGTTT 
CTTTGCTGCA AAGGAGAATT CCTAGTTAAC CGAAACTGTT CATGGAGCGA CTATGTTAAT 
GCATTTAATG GAGATCTGAG ATGGAAAGCA TCAATTAATC ATTATTTGAC TGGAGTTTTA 
AGTATCACTA ACAGCCAAAC CGAGGACAGA CGATGGAGGT ACTACTCATG TGAGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814792.1
CDS	95..2974
Translation	MLAPAPPDHFKMAAVSGGSTEGPSLLNGDLLESETGGLGGAGEMCASGGYPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYVEAYEEYTSKLDLLQQREQQLLESMGNGTDSTSLPSELTLSSVPSNLSLAPSALSALRTPSDPSRINPKSPQKPIVRVFLPNKQRTVVPARSGVTVRDSLKKALMMRGLIPECCAVYRVQDGEKKPIGWDTDISWITGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDRLDLLFVSKFFEHHPISQEEAPFEEVAPAAESSTPPPPPDATGPLCLPSTSPSKSIPIPQPFRPDEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGLQCSEGAPSVQPTRCHRKYHSRDPSPLLHTLPSEIVFDFESDPVCRGSTPGLSATPPASLPGSLTNVKALQKSPGQQRERKSSSSSTEDRSRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLRLVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDNNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDFSKVRSNCPKAMKRLMADCLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYTCASPKTPIQAGGYGISPRWVNNFNEPLFYRCPNQQSIKEITSMRDSQRWDRLWDFVCERLFNSVPTCEWSNYVNNIGQGFTFYCPTGQVLSGMGNELDAWESDRRWKFLCCKGEFLVNRNCSWSDYVNAFNGDLRWKASINHYLTGVLSITNSQTEDRRWRYYSCEK

Target ORF information:

RefSeq Version	XM_012959338.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis B-Raf proto-oncogene, serine/threonine kinase (braf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959338.2

ORF Insert Sequence:

ATGCTCGCTC CCGCGCCTCC GGACCACTTC AAGATGGCGG CGGTAAGTGG GGGATCAACC 
GAGGGGCCCT CACTGCTCAA CGGGGACCTG CTGGAGTCCG AGACCGGGGG ACTGGGCGGA 
GCCGGCGAGA TGTGTGCGTC CGGGGGGTAC CCGGAGGAGG TCTGGAATAT AAAGCAGATG 
ATAAAGTTAA CACAAGAGCA CATCGAAGCC CTGCTGGACA AATTTGGAGG CGAGCACAAC 
CCCCCATCTA TATACGTAGA GGCTTATGAG GAATACACCA GCAAACTGGA TCTATTACAG 
CAGAGGGAAC AGCAGTTACT GGAGTCTATG GGCAATGGAA CAGACTCTAC TTCTTTGCCT 
TCAGAGCTGA CACTTTCCTC GGTCCCATCT AATCTATCCT TGGCTCCTTC AGCTCTCTCA 
GCCCTGCGGA CCCCCAGCGA CCCCTCCCGT ATCAATCCCA AGTCTCCCCA GAAACCTATT 
GTCCGAGTCT TCCTTCCCAA CAAGCAGCGC ACTGTGGTAC CAGCTAGGAG TGGTGTGACA 
GTGCGAGACA GCCTAAAGAA AGCCCTGATG ATGCGAGGGT TAATCCCAGA ATGCTGTGCT 
GTGTACAGGG TGCAAGATGG GGAGAAGAAG CCGATTGGCT GGGATACAGA TATAAGCTGG 
ATCACTGGAG AAGAGTTACA CGTGGAAGTC TTGGAGAATG TGCCGCTCAC AACCCATAAC 
TTTGTACGGA AAACCTTCTT CACGCTGGCT TTCTGCGACT TCTGTCGGAA GCTGCTGTTC 
CAGGGCTTCC GCTGCCAGAC GTGCGGGTAC AAGTTCCACC AGCGGTGCAG CACGGAGGTG 
CCTCTTATGT GTGTTAACTA CGACCGTCTA GATTTGCTGT TTGTCTCCAA GTTCTTTGAA 
CACCACCCAA TATCTCAGGA GGAAGCTCCG TTTGAAGAGG TCGCCCCAGC CGCTGAGTCC 
TCGACTCCTC CGCCCCCTCC AGATGCCACA GGCCCCCTCT GTCTACCGAG TACTTCTCCT 
TCCAAATCCA TCCCGATTCC CCAGCCGTTC CGACCGGATG AGGACCACAG GAACCAGTTT 
GGCCAGCGAG ATCGGTCGTC CTCGGCGCCC AACGTGCACA TAAATACCAT CGAGCCGGTC 
AATATTGACG ACTTGATTCG AGACCAGGGC CTCCAGTGCA GCGAGGGAGC CCCCTCAGTC 
CAGCCGACGC GCTGCCATCG GAAATACCAC TCGCGGGACC CCAGCCCACT CCTCCATACT 
CTCCCCAGTG AAATAGTCTT TGATTTTGAA TCTGATCCTG TTTGCAGAGG TTCAACCCCC 
GGCTTATCGG CGACCCCCCC TGCCTCCCTC CCCGGCTCCC TCACCAACGT CAAGGCGCTA 
CAGAAATCCC CGGGGCAGCA GAGAGAGCGC AAGTCCTCCT CGTCCTCGAC GGAAGACAGG 
AGTCGGATGA AAACGCTCGG ACGACGCGAT TCCAGCGACG ACTGGGAGAT TCCCGACGGG 
CAAATCACGG TTGGGCAGAG AATAGGTTCT GGATCGTTTG GGACAGTGTA TAAAGGAAAG 
TGGCACGGGG ACGTAGCAGT GAAAATGTTG AATGTAACGG CGCCAACGCC TCAGCAGCTA 
CAGGCCTTTA AAAATGAAGT AGGAGTCCTC AGAAAAACGC GACACGTGAA CATCCTGCTT 
TTCATGGGCT ACTCCACGAA GCCCCAGCTG CGACTAGTGA CCCAGTGGTG CGAGGGCTCC 
AGTCTCTATC ACCACCTGCA CATCATCGAG ACCAAATTCG AAATGATCAA GCTGATCGAC 
ATTGCACGAC AGACGGCGCA GGGCATGGAC TATTTGCACG CGAAGTCAAT CATCCACAGA 
GACCTAAAGA GCAATAATAT ATTCCTTCAT GAAGACCTCA CAGTAAAGAT TGGTGACTTT 
GGCCTGGCAA CAGTGAAGTC CCGGTGGAGT GGGTCTCATC AGTTTGAGCA GTTGTCTGGC 
TCCATTCTGT GGATGGCTCC TGAGGTCATT CGGATGCAAG ATAACAACCC CTATAGCTTT 
CAGTCTGATG TCTACGCGTT TGGCATTGTT CTATATGAAC TGATGACGGG GCAGTTGCCC 
TACTCCAACA TCAACAACAG AGACCAGATC ATATTTATGG TTGGGAGAGG CTACTTATCT 
CCCGACTTCA GCAAAGTTAG GAGTAATTGC CCCAAAGCAA TGAAGAGGTT AATGGCAGAC 
TGTCTAAAGA AGAAGAGAGA TGAGCGCCCC CTATTCCCAC AGATTCTCGC CTCCATCGAG 
CTGCTGGCAC GGTCATTGCC AAAAATCCAC CGCAGCGCGT CTGAGCCGTC CTTAAACCGC 
GCTGGATTCC AGACGGAAGA CTTCAGTCTG TACACGTGCG CTTCTCCCAA AACGCCTATC 
CAGGCGGGAG GTTATGGAAT CTCACCAAGA TGGGTAAATA ACTTTAATGA ACCTCTTTTT 
TATCGGTGTC CAAATCAGCA GAGTATCAAA GAAATCACAA GTATGCGTGA CAGTCAGAGG 
TGGGACCGAC TATGGGATTT TGTCTGTGAG AGATTATTCA ACTCCGTTCC TACTTGTGAA 
TGGAGCAACT ATGTCAATAA CATTGGCCAA GGATTTACCT TCTATTGCCC AACTGGCCAG 
GTTCTCAGTG GAATGGGAAA TGAACTTGAT GCCTGGGAGT CAGACAGGAG GTGGAAGTTT 
CTTTGCTGCA AAGGAGAATT CCTAGTTAAC CGAAACTGTT CATGGAGCGA CTATGTTAAT 
GCATTTAATG GAGATCTGAG ATGGAAAGCA TCAATTAATC ATTATTTGAC TGGAGTTTTA 
AGTATCACTA ACAGCCAAAC CGAGGACAGA CGATGGAGGT ACTACTCATG TGAGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

braf ( XM_031898528.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754388.1 Xenopus tropicalis B-Raf proto-oncogene, serine/threonine kinase (braf), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa54165
Clone ID Related Accession (Same CDS sequence)	XM_031898528.1
Accession Version	XM_031898528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase B-raf isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGCTC CCGCGCCTCC GGACCACTTC AAGATGGCGG CGGTAAGTGG GGGATCAACC 
GAGGGGCCCT CACTGCTCAA CGGGGACCTG CTGGAGTCCG AGACCGGGGG ACTGGGCGGA 
GCCGGCGAGA TGTGTGCGTC CGGGGGGTAC CCGGAGGAGG TCTGGAATAT AAAGCAGATG 
ATAAAGTTAA CACAAGAGCA CATCGAAGCC CTGCTGGACA AATTTGGAGG CGAGCACAAC 
CCCCCATCTA TATACGTAGA GGCTTATGAG GAATACACCA GCAAACTGGA TCTATTACAG 
CAGAGGGAAC AGCAGTTACT GGAGTCTATG GGCAATGGAA CAGACTCTAC TTCTTTGCCT 
TCAGAGCTGA CACTTTCCTC GGTCCCATCT AATCTATCCT TGGCTCCTTC AGCTCTCTCA 
GCCCTGCGGA CCCCCAGCGA CCCCTCCCGT ATCAATCCCA AGTCTCCCCA GAAACCTATT 
GTCCGAGTCT TCCTTCCCAA CAAGCAGCGC ACTGTGGTAC CAGCTAGGAG TGGTGTGACA 
GTGCGAGACA GCCTAAAGAA AGCCCTGATG ATGCGAGGGT TAATCCCAGA ATGCTGTGCT 
GTGTACAGGG TGCAAGATGG GGAGAAGAAG CCGATTGGCT GGGATACAGA TATAAGCTGG 
ATCACTGGAG AAGAGTTACA CGTGGAAGTC TTGGAGAATG TGCCGCTCAC AACCCATAAC 
TTTGTACGGA AAACCTTCTT CACGCTGGCT TTCTGCGACT TCTGTCGGAA GCTGCTGTTC 
CAGGGCTTCC GCTGCCAGAC GTGCGGGTAC AAGTTCCACC AGCGGTGCAG CACGGAGGTG 
CCTCTTATGT GTGTTAACTA CGACCGTCTA GATTTGCTGT TTGTCTCCAA GTTCTTTGAA 
CACCACCCAA TATCTCAGGA GGAAGCTCCG TTTGAAGAGG TCGCCCCAGC CGCTGAGTCC 
TCGACTCCTC CGCCCCCTCC AGATGCCACA GGCCCCCTCT GTCTACCGAG TACTTCTCCT 
TCCAAATCCA TCCCGATTCC CCAGCCGTTC CGACCGGATG AGGACCACAG GAACCAGTTT 
GGCCAGCGAG ATCGGTCGTC CTCGGCGCCC AACGTGCACA TAAATACCAT CGAGCCGGTC 
AATATTGACG ACTTGATTCG AGACCAGGGC CTCCAGTGCA GCGAGGGAGC CCCCTCAGTC 
CAGCCGACGC GCTGCCATCG GAAATACCAC TCGCGGGACC CCAGCCCACT CCTCCATACT 
CTCCCCAGTG AAATAGTCTT TGATTTTGAA TCTGATCCTG TTTGCAGAGG TTCAACCCCC 
GGCTTATCGG CGACCCCCCC TGCCTCCCTC CCCGGCTCCC TCACCAACGT CAAGGCGCTA 
CAGAAATCCC CGGGGCAGCA GAGAGAGCGC AAGTCCTCCT CGTCCTCGAC GGAAGACAGG 
AGTCGGATGA AAACGCTCGG ACGACGCGAT TCCAGCGACG ACTGGGAGAT TCCCGACGGG 
CAAATCACGG TTGGGCAGAG AATAGGTTCT GGATCGTTTG GGACAGTGTA TAAGGGAAAG 
TGGCACGGGG ACGTAGCAGT GAAAATGTTG AATGTAACGG CGCCAACGCC TCAGCAGCTA 
CAGGCCTTTA AAAATGAAGT AGGAGTCCTC AGAAAAACGC GACACGTGAA CATCCTGCTT 
TTCATGGGCT ACTCCACGAA GCCCCAGCTG CGACTAGTGA CCCAGTGGTG CGAGGGCTCC 
AGTCTCTATC ACCACCTGCA CATCATCGAG ACCAAATTCG AAATGATCAA GCTGATCGAC 
ATTGCACGAC AGACGGCGCA GGGCATGGAC TATTTGCACG CGAAGTCAAT CATCCACAGA 
GACCTAAAGA GCAATAATAT ATTCCTTCAT GAAGACCTCA CAGTAAAGAT TGGTGACTTT 
GGCCTGGCAA CAGTGAAGTC CCGGTGGAGT GGGTCTCATC AGTTTGAGCA GTTGTCTGGC 
TCCATTCTGT GGATGGCTCC TGAGGTCATT CGGATGCAAG ATAACAACCC CTATAGCTTT 
CAGTCTGATG TCTACGCGTT TGGCATTGTT CTATATGAAC TGATGACGGG GCAGTTGCCC 
TACTCCAACA TCAACAACAG AGACCAGATC ATATTTATGG TTGGGAGAGG CTACTTATCT 
CCCGACTTCA GCAAAGTTAG GAGTAATTGC CCCAAAGCAA TGAAGAGGTT AATGGCAGAC 
TGTCTAAAGA AGAAGAGAGA TGAGCGCCCC CTATTCCCAC AGATTCTCGC CTCCATCGAG 
CTGCTGGCAC GGTCATTGCC AAAAATCCAC CGCAGCGCGT CTGAGCCGTC CTTAAACCGC 
GCTGGATTCC AGACGGAAGA CTTCAGTCTG TACACGTGCG CTTCTCCCAA AACGCCTATC 
CAGGCGGGAG GTTATGAAAT CTCACCAAGA TGGGTAAATA ACTTTAATGA ACCTCTTTTT 
TATCGGTGTC CAAATCAGCA GAGTATCAAA GAAATCACAA GTATGCGTGA CAGTCAGAGG 
TGGGACCGAC TATGGGATTT TGTCTGTGAG AGATTATTCA ACTCCGTTCC TACTTGTGAA 
TGGAGCAACT ACGTCAATAA CATTGGCCAA GGATTTACCT TCTATTGCCC AACTGGCCAG 
GTTCTCAGTG GAATGGGAAA TGAACTTGAT GCCTGGGAGT CAGACAGGAG ATTGTTCCAC 
AGGTGGAAGT TTCTTTGCTG CAAAGGAGAA TTCCTACTTA ACCGAAACTG TTCATGGAGC 
GACTATGTTA ATGCATTTAA TGAAGATCTG AGATGGAAAG CATCAATTAA TCATTATTTG 
GCTGGAGTTT TAAGTATCAC TAACAGCCAA ACCGAGGACA GACGATGGAG GTACTACTCA 
TGTGAGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754388.1
CDS	90..2981
Translation	MLAPAPPDHFKMAAVSGGSTEGPSLLNGDLLESETGGLGGAGEMCASGGYPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYVEAYEEYTSKLDLLQQREQQLLESMGNGTDSTSLPSELTLSSVPSNLSLAPSALSALRTPSDPSRINPKSPQKPIVRVFLPNKQRTVVPARSGVTVRDSLKKALMMRGLIPECCAVYRVQDGEKKPIGWDTDISWITGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDRLDLLFVSKFFEHHPISQEEAPFEEVAPAAESSTPPPPPDATGPLCLPSTSPSKSIPIPQPFRPDEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGLQCSEGAPSVQPTRCHRKYHSRDPSPLLHTLPSEIVFDFESDPVCRGSTPGLSATPPASLPGSLTNVKALQKSPGQQRERKSSSSSTEDRSRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLRLVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDNNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDFSKVRSNCPKAMKRLMADCLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYTCASPKTPIQAGGYEISPRWVNNFNEPLFYRCPNQQSIKEITSMRDSQRWDRLWDFVCERLFNSVPTCEWSNYVNNIGQGFTFYCPTGQVLSGMGNELDAWESDRRLFHRWKFLCCKGEFLLNRNCSWSDYVNAFNEDLRWKASINHYLAGVLSITNSQTEDRRWRYYSCEK

Target ORF information:

RefSeq Version	XM_031898528.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis B-Raf proto-oncogene, serine/threonine kinase (braf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898528.1

ORF Insert Sequence:

ATGCTCGCTC CCGCGCCTCC GGACCACTTC AAGATGGCGG CGGTAAGTGG GGGATCAACC 
GAGGGGCCCT CACTGCTCAA CGGGGACCTG CTGGAGTCCG AGACCGGGGG ACTGGGCGGA 
GCCGGCGAGA TGTGTGCGTC CGGGGGGTAC CCGGAGGAGG TCTGGAATAT AAAGCAGATG 
ATAAAGTTAA CACAAGAGCA CATCGAAGCC CTGCTGGACA AATTTGGAGG CGAGCACAAC 
CCCCCATCTA TATACGTAGA GGCTTATGAG GAATACACCA GCAAACTGGA TCTATTACAG 
CAGAGGGAAC AGCAGTTACT GGAGTCTATG GGCAATGGAA CAGACTCTAC TTCTTTGCCT 
TCAGAGCTGA CACTTTCCTC GGTCCCATCT AATCTATCCT TGGCTCCTTC AGCTCTCTCA 
GCCCTGCGGA CCCCCAGCGA CCCCTCCCGT ATCAATCCCA AGTCTCCCCA GAAACCTATT 
GTCCGAGTCT TCCTTCCCAA CAAGCAGCGC ACTGTGGTAC CAGCTAGGAG TGGTGTGACA 
GTGCGAGACA GCCTAAAGAA AGCCCTGATG ATGCGAGGGT TAATCCCAGA ATGCTGTGCT 
GTGTACAGGG TGCAAGATGG GGAGAAGAAG CCGATTGGCT GGGATACAGA TATAAGCTGG 
ATCACTGGAG AAGAGTTACA CGTGGAAGTC TTGGAGAATG TGCCGCTCAC AACCCATAAC 
TTTGTACGGA AAACCTTCTT CACGCTGGCT TTCTGCGACT TCTGTCGGAA GCTGCTGTTC 
CAGGGCTTCC GCTGCCAGAC GTGCGGGTAC AAGTTCCACC AGCGGTGCAG CACGGAGGTG 
CCTCTTATGT GTGTTAACTA CGACCGTCTA GATTTGCTGT TTGTCTCCAA GTTCTTTGAA 
CACCACCCAA TATCTCAGGA GGAAGCTCCG TTTGAAGAGG TCGCCCCAGC CGCTGAGTCC 
TCGACTCCTC CGCCCCCTCC AGATGCCACA GGCCCCCTCT GTCTACCGAG TACTTCTCCT 
TCCAAATCCA TCCCGATTCC CCAGCCGTTC CGACCGGATG AGGACCACAG GAACCAGTTT 
GGCCAGCGAG ATCGGTCGTC CTCGGCGCCC AACGTGCACA TAAATACCAT CGAGCCGGTC 
AATATTGACG ACTTGATTCG AGACCAGGGC CTCCAGTGCA GCGAGGGAGC CCCCTCAGTC 
CAGCCGACGC GCTGCCATCG GAAATACCAC TCGCGGGACC CCAGCCCACT CCTCCATACT 
CTCCCCAGTG AAATAGTCTT TGATTTTGAA TCTGATCCTG TTTGCAGAGG TTCAACCCCC 
GGCTTATCGG CGACCCCCCC TGCCTCCCTC CCCGGCTCCC TCACCAACGT CAAGGCGCTA 
CAGAAATCCC CGGGGCAGCA GAGAGAGCGC AAGTCCTCCT CGTCCTCGAC GGAAGACAGG 
AGTCGGATGA AAACGCTCGG ACGACGCGAT TCCAGCGACG ACTGGGAGAT TCCCGACGGG 
CAAATCACGG TTGGGCAGAG AATAGGTTCT GGATCGTTTG GGACAGTGTA TAAGGGAAAG 
TGGCACGGGG ACGTAGCAGT GAAAATGTTG AATGTAACGG CGCCAACGCC TCAGCAGCTA 
CAGGCCTTTA AAAATGAAGT AGGAGTCCTC AGAAAAACGC GACACGTGAA CATCCTGCTT 
TTCATGGGCT ACTCCACGAA GCCCCAGCTG CGACTAGTGA CCCAGTGGTG CGAGGGCTCC 
AGTCTCTATC ACCACCTGCA CATCATCGAG ACCAAATTCG AAATGATCAA GCTGATCGAC 
ATTGCACGAC AGACGGCGCA GGGCATGGAC TATTTGCACG CGAAGTCAAT CATCCACAGA 
GACCTAAAGA GCAATAATAT ATTCCTTCAT GAAGACCTCA CAGTAAAGAT TGGTGACTTT 
GGCCTGGCAA CAGTGAAGTC CCGGTGGAGT GGGTCTCATC AGTTTGAGCA GTTGTCTGGC 
TCCATTCTGT GGATGGCTCC TGAGGTCATT CGGATGCAAG ATAACAACCC CTATAGCTTT 
CAGTCTGATG TCTACGCGTT TGGCATTGTT CTATATGAAC TGATGACGGG GCAGTTGCCC 
TACTCCAACA TCAACAACAG AGACCAGATC ATATTTATGG TTGGGAGAGG CTACTTATCT 
CCCGACTTCA GCAAAGTTAG GAGTAATTGC CCCAAAGCAA TGAAGAGGTT AATGGCAGAC 
TGTCTAAAGA AGAAGAGAGA TGAGCGCCCC CTATTCCCAC AGATTCTCGC CTCCATCGAG 
CTGCTGGCAC GGTCATTGCC AAAAATCCAC CGCAGCGCGT CTGAGCCGTC CTTAAACCGC 
GCTGGATTCC AGACGGAAGA CTTCAGTCTG TACACGTGCG CTTCTCCCAA AACGCCTATC 
CAGGCGGGAG GTTATGAAAT CTCACCAAGA TGGGTAAATA ACTTTAATGA ACCTCTTTTT 
TATCGGTGTC CAAATCAGCA GAGTATCAAA GAAATCACAA GTATGCGTGA CAGTCAGAGG 
TGGGACCGAC TATGGGATTT TGTCTGTGAG AGATTATTCA ACTCCGTTCC TACTTGTGAA 
TGGAGCAACT ACGTCAATAA CATTGGCCAA GGATTTACCT TCTATTGCCC AACTGGCCAG 
GTTCTCAGTG GAATGGGAAA TGAACTTGAT GCCTGGGAGT CAGACAGGAG ATTGTTCCAC 
AGGTGGAAGT TTCTTTGCTG CAAAGGAGAA TTCCTACTTA ACCGAAACTG TTCATGGAGC 
GACTATGTTA ATGCATTTAA TGAAGATCTG AGATGGAAAG CATCAATTAA TCATTATTTG 
GCTGGAGTTT TAAGTATCAC TAACAGCCAA ACCGAGGACA GACGATGGAG GTACTACTCA 
TGTGAGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

braf ( XM_031898529.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754389.1 Xenopus tropicalis B-Raf proto-oncogene, serine/threonine kinase (braf), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa54166
Clone ID Related Accession (Same CDS sequence)	XM_031898529.1
Accession Version	XM_031898529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase B-raf isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGCTC CCGCGCCTCC GGACCACTTC AAGATGGCGG CGGTAAGTGG GGGATCAACC 
GAGGGGCCCT CACTGCTCAA CGGGGACCTG CTGGAGTCCG AGACCGGGGG ACTGGGCGGA 
GCCGGCGAGA TGTGTGCGTC CGGGGGGTAC CCGGAGGAGG TCTGGAATAT AAAGCAGATG 
ATAAAGTTAA CACAAGAGCA CATCGAAGCC CTGCTGGACA AATTTGGAGG CGAGCACAAC 
CCCCCATCTA TATACGTAGA GGCTTATGAG GAATACACCA GCAAACTGGA TCTATTACAG 
CAGAGGGAAC AGCAGTTACT GGAGTCTATG GGCAATGGAA CAGACTCTAC TTCTTTGCCT 
TCAGAGCTGA CACTTTCCTC GGTCCCATCT AATCTATCCT TGGCTCCTTC AGCTCTCTCA 
GCCCTGCGGA CCCCCAGCGA CCCCTCCCGT ATCAATCCCA AGTCTCCCCA GAAACCTATT 
GTCCGAGTCT TCCTTCCCAA CAAGCAGCGC ACTGTGGTAC CAGCTAGGAG TGGTGTGACA 
GTGCGAGACA GCCTAAAGAA AGCCCTGATG ATGCGAGGGT TAATCCCAGA ATGCTGTGCT 
GTGTACAGGG TGCAAGATGG GGAGAAGAAG CCGATTGGCT GGGATACAGA TATAAGCTGG 
ATCACTGGAG AAGAGTTACA CGTGGAAGTC TTGGAGAATG TGCCGCTCAC AACCCATAAC 
TTTGTACGGA AAACCTTCTT CACGCTGGCT TTCTGCGACT TCTGTCGGAA GCTGCTGTTC 
CAGGGCTTCC GCTGCCAGAC GTGCGGGTAC AAGTTCCACC AGCGGTGCAG CACGGAGGTG 
CCTCTTATGT GTGTTAACTA CGACCGTCTA GATTTGCTGT TTGTCTCCAA GTTCTTTGAA 
CACCACCCAA TATCTCAGGA GGAAGCTCCG TTTGAAGAGG TCGCCCCAGC CGCTGAGTCC 
TCGACTCCTC CGCCCCCTCC AGATGCCACA GGCCCCCTCT GTCTACCGAG TACTTCTCCT 
TCCAAATCCA TCCCGATTCC CCAGCCGTTC CGACCGGATG AGGACCACAG GAACCAGTTT 
GGCCAGCGAG ATCGGTCGTC CTCGGCGCCC AACGTGCACA TAAATACCAT CGAGCCGGTC 
AATATTGACG ACTTGATTCG AGACCAGGGC CTCCAGTGCA GCGAGGGAGC CCCCTCAGTC 
CAGCCGACGC GCTGCCATCG GAAATACCAC TCGCGGGACC CCAGCCCACT CCTCCATACT 
CTCCCCAGTG AAATAGTCTT TGATTTTGAA TCTGATCCTG TTTGCAGAGG TTCAACCCCC 
GGCTTATCGG CGACCCCCCC TGCCTCCCTC CCCGGCTCCC TCACCAACGT CAAGGCGCTA 
CAGAAATCCC CGGGGCAGCA GAGAGAGCGC AAGTCCTCCT CGTCCTCGAC GGAAGACAGG 
AGTCGGATGA AAACGCTCGG ACGACGCGAT TCCAGCGACG ACTGGGAGAT TCCCGACGGG 
CAAATCACGG TTGGGCAGAG AATAGGTTCT GGATCGTTTG GGACAGTGTA TAAGGGAAAG 
TGGCACGGGG ACGTAGCAGT GAAAATGTTG AATGTAACGG CGCCAACGCC TCAGCAGCTA 
CAGGCCTTTA AAAATGAAGT AGGAGTCCTC AGAAAAACGC GACACGTGAA CATCCTGCTT 
TTCATGGGCT ACTCCACGAA GCCCCAGCTG CGACTAGTGA CCCAGTGGTG CGAGGGCTCC 
AGTCTCTATC ACCACCTGCA CATCATCGAG ACCAAATTCG AAATGATCAA GCTGATCGAC 
ATTGCACGAC AGACGGCGCA GGGCATGGAC TATTTGCACG CGAAGTCAAT CATCCACAGA 
GACCTAAAGA GCAATAATAT ATTCCTTCAT GAAGACCTCA CAGTAAAGAT TGGTGACTTT 
GGCCTGGCAA CAGTGAAGTC CCGGTGGAGT GGGTCTCATC AGTTTGAGCA GTTGTCTGGC 
TCCATTCTGT GGATGGCTCC TGAGGTCATT CGGATGCAAG ATAACAACCC CTATAGCTTT 
CAGTCTGATG TCTACGCGTT TGGCATTGTT CTATATGAAC TGATGACGGG GCAGTTGCCC 
TACTCCAACA TCAACAACAG AGACCAGATC ATATTTATGG TTGGGAGAGG CTACTTATCT 
CCCGACTTCA GCAAAGTTAG GAGTAATTGC CCCAAAGCAA TGAAGAGGTT AATGGCAGAC 
TGTCTAAAGA AGAAGAGAGA TGAGCGCCCC CTATTCCCAC AGATTCTCGC CTCCATCGAG 
CTGCTGGCAC GGTCATTGCC AAAAATCCAC CGCAGCGCGT CTGAGCCGTC CTTAAACCGC 
GCTGGATTCC AGACGGAAGA CTTCAGTCTG TACACGTGCG CTTCTCCCAA AACGCCTATC 
CAGGCGGGAG GTTATGAAAT CTCACCAAGA TGGGTAAATA ACTTTAATGA ACCTCTTTTT 
TATCGGTGTC CAAATCAGCA GAGTATCAAA GAAATCACAA GTATGCGTGA CAGTCAGAGG 
TGGGACCGAC TATGGGATTT TGTCTGTGAG AGATTATTCA ACTCCGTTCC TACTTGTGAA 
TGGAGCAACT ACGTCAATAA CATTGGCCAA GGATTTACCT TCTATTGCCC AACTGGCCAG 
GTTCTCAGTG GAATGGGAAA TGAACTTGAT GCCTGGGAGT CAGACAGGAG ATTGTTCCAC 
AGGTGGAAGT TTCTTTGCTG CAAAGGAGAA TTCCTACTTA ACCGAAACTG TTCATGGAGC 
GACTATGTTA ATGCATTTAA TGAAGATCTG AGATGGAAAG CATCAATTAA TCATTATTTG 
GCTGGAGTTT TAAGTATCAC TAACAGCCAA ACCGAGGACA GACAATGGAA GTACTACTCA 
TGTGAGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754389.1
CDS	90..2981
Translation	MLAPAPPDHFKMAAVSGGSTEGPSLLNGDLLESETGGLGGAGEMCASGGYPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYVEAYEEYTSKLDLLQQREQQLLESMGNGTDSTSLPSELTLSSVPSNLSLAPSALSALRTPSDPSRINPKSPQKPIVRVFLPNKQRTVVPARSGVTVRDSLKKALMMRGLIPECCAVYRVQDGEKKPIGWDTDISWITGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDRLDLLFVSKFFEHHPISQEEAPFEEVAPAAESSTPPPPPDATGPLCLPSTSPSKSIPIPQPFRPDEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGLQCSEGAPSVQPTRCHRKYHSRDPSPLLHTLPSEIVFDFESDPVCRGSTPGLSATPPASLPGSLTNVKALQKSPGQQRERKSSSSSTEDRSRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLRLVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDNNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDFSKVRSNCPKAMKRLMADCLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYTCASPKTPIQAGGYEISPRWVNNFNEPLFYRCPNQQSIKEITSMRDSQRWDRLWDFVCERLFNSVPTCEWSNYVNNIGQGFTFYCPTGQVLSGMGNELDAWESDRRLFHRWKFLCCKGEFLLNRNCSWSDYVNAFNEDLRWKASINHYLAGVLSITNSQTEDRQWKYYSCEK

Target ORF information:

RefSeq Version	XM_031898529.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis B-Raf proto-oncogene, serine/threonine kinase (braf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898529.1

ORF Insert Sequence:

ATGCTCGCTC CCGCGCCTCC GGACCACTTC AAGATGGCGG CGGTAAGTGG GGGATCAACC 
GAGGGGCCCT CACTGCTCAA CGGGGACCTG CTGGAGTCCG AGACCGGGGG ACTGGGCGGA 
GCCGGCGAGA TGTGTGCGTC CGGGGGGTAC CCGGAGGAGG TCTGGAATAT AAAGCAGATG 
ATAAAGTTAA CACAAGAGCA CATCGAAGCC CTGCTGGACA AATTTGGAGG CGAGCACAAC 
CCCCCATCTA TATACGTAGA GGCTTATGAG GAATACACCA GCAAACTGGA TCTATTACAG 
CAGAGGGAAC AGCAGTTACT GGAGTCTATG GGCAATGGAA CAGACTCTAC TTCTTTGCCT 
TCAGAGCTGA CACTTTCCTC GGTCCCATCT AATCTATCCT TGGCTCCTTC AGCTCTCTCA 
GCCCTGCGGA CCCCCAGCGA CCCCTCCCGT ATCAATCCCA AGTCTCCCCA GAAACCTATT 
GTCCGAGTCT TCCTTCCCAA CAAGCAGCGC ACTGTGGTAC CAGCTAGGAG TGGTGTGACA 
GTGCGAGACA GCCTAAAGAA AGCCCTGATG ATGCGAGGGT TAATCCCAGA ATGCTGTGCT 
GTGTACAGGG TGCAAGATGG GGAGAAGAAG CCGATTGGCT GGGATACAGA TATAAGCTGG 
ATCACTGGAG AAGAGTTACA CGTGGAAGTC TTGGAGAATG TGCCGCTCAC AACCCATAAC 
TTTGTACGGA AAACCTTCTT CACGCTGGCT TTCTGCGACT TCTGTCGGAA GCTGCTGTTC 
CAGGGCTTCC GCTGCCAGAC GTGCGGGTAC AAGTTCCACC AGCGGTGCAG CACGGAGGTG 
CCTCTTATGT GTGTTAACTA CGACCGTCTA GATTTGCTGT TTGTCTCCAA GTTCTTTGAA 
CACCACCCAA TATCTCAGGA GGAAGCTCCG TTTGAAGAGG TCGCCCCAGC CGCTGAGTCC 
TCGACTCCTC CGCCCCCTCC AGATGCCACA GGCCCCCTCT GTCTACCGAG TACTTCTCCT 
TCCAAATCCA TCCCGATTCC CCAGCCGTTC CGACCGGATG AGGACCACAG GAACCAGTTT 
GGCCAGCGAG ATCGGTCGTC CTCGGCGCCC AACGTGCACA TAAATACCAT CGAGCCGGTC 
AATATTGACG ACTTGATTCG AGACCAGGGC CTCCAGTGCA GCGAGGGAGC CCCCTCAGTC 
CAGCCGACGC GCTGCCATCG GAAATACCAC TCGCGGGACC CCAGCCCACT CCTCCATACT 
CTCCCCAGTG AAATAGTCTT TGATTTTGAA TCTGATCCTG TTTGCAGAGG TTCAACCCCC 
GGCTTATCGG CGACCCCCCC TGCCTCCCTC CCCGGCTCCC TCACCAACGT CAAGGCGCTA 
CAGAAATCCC CGGGGCAGCA GAGAGAGCGC AAGTCCTCCT CGTCCTCGAC GGAAGACAGG 
AGTCGGATGA AAACGCTCGG ACGACGCGAT TCCAGCGACG ACTGGGAGAT TCCCGACGGG 
CAAATCACGG TTGGGCAGAG AATAGGTTCT GGATCGTTTG GGACAGTGTA TAAGGGAAAG 
TGGCACGGGG ACGTAGCAGT GAAAATGTTG AATGTAACGG CGCCAACGCC TCAGCAGCTA 
CAGGCCTTTA AAAATGAAGT AGGAGTCCTC AGAAAAACGC GACACGTGAA CATCCTGCTT 
TTCATGGGCT ACTCCACGAA GCCCCAGCTG CGACTAGTGA CCCAGTGGTG CGAGGGCTCC 
AGTCTCTATC ACCACCTGCA CATCATCGAG ACCAAATTCG AAATGATCAA GCTGATCGAC 
ATTGCACGAC AGACGGCGCA GGGCATGGAC TATTTGCACG CGAAGTCAAT CATCCACAGA 
GACCTAAAGA GCAATAATAT ATTCCTTCAT GAAGACCTCA CAGTAAAGAT TGGTGACTTT 
GGCCTGGCAA CAGTGAAGTC CCGGTGGAGT GGGTCTCATC AGTTTGAGCA GTTGTCTGGC 
TCCATTCTGT GGATGGCTCC TGAGGTCATT CGGATGCAAG ATAACAACCC CTATAGCTTT 
CAGTCTGATG TCTACGCGTT TGGCATTGTT CTATATGAAC TGATGACGGG GCAGTTGCCC 
TACTCCAACA TCAACAACAG AGACCAGATC ATATTTATGG TTGGGAGAGG CTACTTATCT 
CCCGACTTCA GCAAAGTTAG GAGTAATTGC CCCAAAGCAA TGAAGAGGTT AATGGCAGAC 
TGTCTAAAGA AGAAGAGAGA TGAGCGCCCC CTATTCCCAC AGATTCTCGC CTCCATCGAG 
CTGCTGGCAC GGTCATTGCC AAAAATCCAC CGCAGCGCGT CTGAGCCGTC CTTAAACCGC 
GCTGGATTCC AGACGGAAGA CTTCAGTCTG TACACGTGCG CTTCTCCCAA AACGCCTATC 
CAGGCGGGAG GTTATGAAAT CTCACCAAGA TGGGTAAATA ACTTTAATGA ACCTCTTTTT 
TATCGGTGTC CAAATCAGCA GAGTATCAAA GAAATCACAA GTATGCGTGA CAGTCAGAGG 
TGGGACCGAC TATGGGATTT TGTCTGTGAG AGATTATTCA ACTCCGTTCC TACTTGTGAA 
TGGAGCAACT ACGTCAATAA CATTGGCCAA GGATTTACCT TCTATTGCCC AACTGGCCAG 
GTTCTCAGTG GAATGGGAAA TGAACTTGAT GCCTGGGAGT CAGACAGGAG ATTGTTCCAC 
AGGTGGAAGT TTCTTTGCTG CAAAGGAGAA TTCCTACTTA ACCGAAACTG TTCATGGAGC 
GACTATGTTA ATGCATTTAA TGAAGATCTG AGATGGAAAG CATCAATTAA TCATTATTTG 
GCTGGAGTTT TAAGTATCAC TAACAGCCAA ACCGAGGACA GACAATGGAA GTACTACTCA 
TGTGAGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

braf ( XM_031898530.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754390.1 Xenopus tropicalis B-Raf proto-oncogene, serine/threonine kinase (braf), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa54167
Clone ID Related Accession (Same CDS sequence)	XM_031898530.1
Accession Version	XM_031898530.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase B-raf isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGCTC CCGCGCCTCC GGACCACTTC AAGATGGCGG CGGTAAGTGG GGGATCAACC 
GAGGGGCCCT CACTGCTCAA CGGGGACCTG CTGGAGTCCG AGACCGGGGG ACTGGGCGGA 
GCCGGCGAGA TGTGTGCGTC CGGGGGGTAC CCGGAGGAGG TCTGGAATAT AAAGCAGATG 
ATAAAGTTAA CACAAGAGCA CATCGAAGCC CTGCTGGACA AATTTGGAGG CGAGCACAAC 
CCCCCATCTA TATACGTAGA GGCTTATGAG GAATACACCA GCAAACTGGA TCTATTACAG 
CAGAGGGAAC AGCAGTTACT GGAGTCTATG GGCAATGGAA CAGACTCTAC TTCTTTGCCT 
TCAGAGCTGA CACTTTCCTC GGTCCCATCT AATCTATCCT TGGCTCCTTC AGCTCTCTCA 
GCCCTGCGGA CCCCCAGCGA CCCCTCCCGT ATCAATCCCA AGTCTCCCCA GAAACCTATT 
GTCCGAGTCT TCCTTCCCAA CAAGCAGCGC ACTGTGGTAC CAGCTAGGAG TGGTGTGACA 
GTGCGAGACA GCCTAAAGAA AGCCCTGATG ATGCGAGGGT TAATCCCAGA ATGCTGTGCT 
GTGTACAGGG TGCAAGATGG GGAGAAGAAG CCGATTGGCT GGGATACAGA TATAAGCTGG 
ATCACTGGAG AAGAGTTACA CGTGGAAGTC TTGGAGAATG TGCCGCTCAC AACCCATAAC 
TTTGTACGGA AAACCTTCTT CACGCTGGCT TTCTGCGACT TCTGTCGGAA GCTGCTGTTC 
CAGGGCTTCC GCTGCCAGAC GTGCGGGTAC AAGTTCCACC AGCGGTGCAG CACGGAGGTG 
CCTCTTATGT GTGTTAACTA CGACCGTCTA GATTTGCTGT TTGTCTCCAA GTTCTTTGAA 
CACCACCCAA TATCTCAGGA GGAAGCTCCG TTTGAAGAGG TCGCCCCAGC CGCTGAGTCC 
TCGACTCCTC CGCCCCCTCC AGATGCCACA GGCCCCCTCT GTCTACCGAG TACTTCTCCT 
TCCAAATCCA TCCCGATTCC CCAGCCGTTC CGACCGGATG AGGACCACAG GAACCAGTTT 
GGCCAGCGAG ATCGGTCGTC CTCGGCGCCC AACGTGCACA TAAATACCAT CGAGCCGGTC 
AATATTGACG ACTTGATTCG AGACCAGGGC CTCCAGTGCA GCGAGGGAGC CCCCTCAGTC 
CAGCCGACGC GCTGCCATCG GAAATACCAC TCGCGGGACC CCAGCCCACT CCTCCATACT 
CTCCCCAGTG AAATAGTCTT TGATTTTGAA TCTGATCCTG TTTGCAGAGG TTCAACCCCC 
GGCTTATCGG CGACCCCCCC TGCCTCCCTC CCCGGCTCCC TCACCAACGT CAAGGCGCTA 
CAGAAATCCC CGGGGCAGCA GAGAGAGCGC AAGTCCTCCT CGTCCTCGAC GGAAGACAGG 
AGTCGGATGA AAACGCTCGG ACGACGCGAT TCCAGCGACG ACTGGGAGAT TCCCGACGGG 
CAAATCACGG TTGGGCAGAG AATAGGTTCT GGATCGTTTG GGACAGTGTA TAAGGGAAAG 
TGGCACGGGG ACGTAGCAGT GAAAATGTTG AATGTAACGG CGCCAACGCC TCAGCAGCTA 
CAGGCCTTTA AAAATGAAGT AGGAGTCCTC AGAAAAACGC GACACGTGAA CATCCTGCTT 
TTCATGGGCT ACTCCACGAA GCCCCAGCTG CGACTAGTGA CCCAGTGGTG CGAGGGCTCC 
AGTCTCTATC ACCACCTGCA CATCATCGAG ACCAAATTCG AAATGATCAA GCTGATCGAC 
ATTGCACGAC AGACGGCGCA GGGCATGGAC TATTTGCACG CGAAGTCAAT CATCCACAGA 
GACCTAAAGA GCAATAATAT ATTCCTTCAT GAAGACCTCA CAGTAAAGAT TGGTGACTTT 
GGCCTGGCAA CAGTGAAGTC CCGGTGGAGT GGGTCTCATC AGTTTGAGCA GTTGTCTGGC 
TCCATTCTGT GGATGGCTCC TGAGGTCATT CGGATGCAAG ATAACAACCC CTATAGCTTT 
CAGTCTGATG TCTACGCGTT TGGCATTGTT CTATATGAAC TGATGACGGG GCAGTTGCCC 
TACTCCAACA TCAACAACAG AGACCAGATC ATATTTATGG TTGGGAGAGG CTACTTATCT 
CCCGACTTCA GCAAAGTTAG GAGTAATTGC CCCAAAGCAA TGAAGAGGTT AATGGCAGAC 
TGTCTAAAGA AGAAGAGAGA TGAGCGCCCC CTATTCCCAC AGATTCTCGC CTCCATCGAG 
CTGCTGGCAC GGTCATTGCC AAAAATCCAC CGCAGCGCGT CTGAGCCGTC CTTAAACCGC 
GCTGGATTCC AGACGGAAGA CTTCAGTCTG TACACGTGCG CTTCTCCCAA AACGCCTATC 
CAGGCGGGAG GTTATGAAAT CTCACCAAGA TGGGTAAATA ACTTTAATGA ACCTCTTTTT 
TATCGGTGTC CAAATCAGCA GAGTATCAAA GAAATCACAA GTATGCGTGA CAGTCAGAGG 
TGGGACCGAC TATGGGATTT TGTCTGTGAG AGATTATTCA ACTCCGTTCC TACTTGTGAA 
TGGAGCAACT ACGTCAATAA CATTGGCCAA GGATTTACCT TCTATTGCCC AACTGGCCAG 
GTTCTCAGTG GAATGGGAAA TGAACTTGAT GCCTGGGAGT CAGACAGGAG GTGGAAGTTT 
CTTTGCTGCA AAGGAGAATT CCTACTTAAC CGAAACTGTT CATGGAGCGA CTATGTTAAT 
GCATTTAATG AAGATCTGAG ATGGAAAGCA TCAATTAATC ATTATTTGGC TGGAGTTTTA 
AGTATCACTA ACAGCCAAAC CGAGGACAGA CGATGGAGGT ACTACTCATG TGAGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754390.1
CDS	90..2969
Translation	MLAPAPPDHFKMAAVSGGSTEGPSLLNGDLLESETGGLGGAGEMCASGGYPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYVEAYEEYTSKLDLLQQREQQLLESMGNGTDSTSLPSELTLSSVPSNLSLAPSALSALRTPSDPSRINPKSPQKPIVRVFLPNKQRTVVPARSGVTVRDSLKKALMMRGLIPECCAVYRVQDGEKKPIGWDTDISWITGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDRLDLLFVSKFFEHHPISQEEAPFEEVAPAAESSTPPPPPDATGPLCLPSTSPSKSIPIPQPFRPDEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGLQCSEGAPSVQPTRCHRKYHSRDPSPLLHTLPSEIVFDFESDPVCRGSTPGLSATPPASLPGSLTNVKALQKSPGQQRERKSSSSSTEDRSRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLRLVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDNNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDFSKVRSNCPKAMKRLMADCLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYTCASPKTPIQAGGYEISPRWVNNFNEPLFYRCPNQQSIKEITSMRDSQRWDRLWDFVCERLFNSVPTCEWSNYVNNIGQGFTFYCPTGQVLSGMGNELDAWESDRRWKFLCCKGEFLLNRNCSWSDYVNAFNEDLRWKASINHYLAGVLSITNSQTEDRRWRYYSCEK

Target ORF information:

RefSeq Version	XM_031898530.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis B-Raf proto-oncogene, serine/threonine kinase (braf), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898530.1

ORF Insert Sequence:

ATGCTCGCTC CCGCGCCTCC GGACCACTTC AAGATGGCGG CGGTAAGTGG GGGATCAACC 
GAGGGGCCCT CACTGCTCAA CGGGGACCTG CTGGAGTCCG AGACCGGGGG ACTGGGCGGA 
GCCGGCGAGA TGTGTGCGTC CGGGGGGTAC CCGGAGGAGG TCTGGAATAT AAAGCAGATG 
ATAAAGTTAA CACAAGAGCA CATCGAAGCC CTGCTGGACA AATTTGGAGG CGAGCACAAC 
CCCCCATCTA TATACGTAGA GGCTTATGAG GAATACACCA GCAAACTGGA TCTATTACAG 
CAGAGGGAAC AGCAGTTACT GGAGTCTATG GGCAATGGAA CAGACTCTAC TTCTTTGCCT 
TCAGAGCTGA CACTTTCCTC GGTCCCATCT AATCTATCCT TGGCTCCTTC AGCTCTCTCA 
GCCCTGCGGA CCCCCAGCGA CCCCTCCCGT ATCAATCCCA AGTCTCCCCA GAAACCTATT 
GTCCGAGTCT TCCTTCCCAA CAAGCAGCGC ACTGTGGTAC CAGCTAGGAG TGGTGTGACA 
GTGCGAGACA GCCTAAAGAA AGCCCTGATG ATGCGAGGGT TAATCCCAGA ATGCTGTGCT 
GTGTACAGGG TGCAAGATGG GGAGAAGAAG CCGATTGGCT GGGATACAGA TATAAGCTGG 
ATCACTGGAG AAGAGTTACA CGTGGAAGTC TTGGAGAATG TGCCGCTCAC AACCCATAAC 
TTTGTACGGA AAACCTTCTT CACGCTGGCT TTCTGCGACT TCTGTCGGAA GCTGCTGTTC 
CAGGGCTTCC GCTGCCAGAC GTGCGGGTAC AAGTTCCACC AGCGGTGCAG CACGGAGGTG 
CCTCTTATGT GTGTTAACTA CGACCGTCTA GATTTGCTGT TTGTCTCCAA GTTCTTTGAA 
CACCACCCAA TATCTCAGGA GGAAGCTCCG TTTGAAGAGG TCGCCCCAGC CGCTGAGTCC 
TCGACTCCTC CGCCCCCTCC AGATGCCACA GGCCCCCTCT GTCTACCGAG TACTTCTCCT 
TCCAAATCCA TCCCGATTCC CCAGCCGTTC CGACCGGATG AGGACCACAG GAACCAGTTT 
GGCCAGCGAG ATCGGTCGTC CTCGGCGCCC AACGTGCACA TAAATACCAT CGAGCCGGTC 
AATATTGACG ACTTGATTCG AGACCAGGGC CTCCAGTGCA GCGAGGGAGC CCCCTCAGTC 
CAGCCGACGC GCTGCCATCG GAAATACCAC TCGCGGGACC CCAGCCCACT CCTCCATACT 
CTCCCCAGTG AAATAGTCTT TGATTTTGAA TCTGATCCTG TTTGCAGAGG TTCAACCCCC 
GGCTTATCGG CGACCCCCCC TGCCTCCCTC CCCGGCTCCC TCACCAACGT CAAGGCGCTA 
CAGAAATCCC CGGGGCAGCA GAGAGAGCGC AAGTCCTCCT CGTCCTCGAC GGAAGACAGG 
AGTCGGATGA AAACGCTCGG ACGACGCGAT TCCAGCGACG ACTGGGAGAT TCCCGACGGG 
CAAATCACGG TTGGGCAGAG AATAGGTTCT GGATCGTTTG GGACAGTGTA TAAGGGAAAG 
TGGCACGGGG ACGTAGCAGT GAAAATGTTG AATGTAACGG CGCCAACGCC TCAGCAGCTA 
CAGGCCTTTA AAAATGAAGT AGGAGTCCTC AGAAAAACGC GACACGTGAA CATCCTGCTT 
TTCATGGGCT ACTCCACGAA GCCCCAGCTG CGACTAGTGA CCCAGTGGTG CGAGGGCTCC 
AGTCTCTATC ACCACCTGCA CATCATCGAG ACCAAATTCG AAATGATCAA GCTGATCGAC 
ATTGCACGAC AGACGGCGCA GGGCATGGAC TATTTGCACG CGAAGTCAAT CATCCACAGA 
GACCTAAAGA GCAATAATAT ATTCCTTCAT GAAGACCTCA CAGTAAAGAT TGGTGACTTT 
GGCCTGGCAA CAGTGAAGTC CCGGTGGAGT GGGTCTCATC AGTTTGAGCA GTTGTCTGGC 
TCCATTCTGT GGATGGCTCC TGAGGTCATT CGGATGCAAG ATAACAACCC CTATAGCTTT 
CAGTCTGATG TCTACGCGTT TGGCATTGTT CTATATGAAC TGATGACGGG GCAGTTGCCC 
TACTCCAACA TCAACAACAG AGACCAGATC ATATTTATGG TTGGGAGAGG CTACTTATCT 
CCCGACTTCA GCAAAGTTAG GAGTAATTGC CCCAAAGCAA TGAAGAGGTT AATGGCAGAC 
TGTCTAAAGA AGAAGAGAGA TGAGCGCCCC CTATTCCCAC AGATTCTCGC CTCCATCGAG 
CTGCTGGCAC GGTCATTGCC AAAAATCCAC CGCAGCGCGT CTGAGCCGTC CTTAAACCGC 
GCTGGATTCC AGACGGAAGA CTTCAGTCTG TACACGTGCG CTTCTCCCAA AACGCCTATC 
CAGGCGGGAG GTTATGAAAT CTCACCAAGA TGGGTAAATA ACTTTAATGA ACCTCTTTTT 
TATCGGTGTC CAAATCAGCA GAGTATCAAA GAAATCACAA GTATGCGTGA CAGTCAGAGG 
TGGGACCGAC TATGGGATTT TGTCTGTGAG AGATTATTCA ACTCCGTTCC TACTTGTGAA 
TGGAGCAACT ACGTCAATAA CATTGGCCAA GGATTTACCT TCTATTGCCC AACTGGCCAG 
GTTCTCAGTG GAATGGGAAA TGAACTTGAT GCCTGGGAGT CAGACAGGAG GTGGAAGTTT 
CTTTGCTGCA AAGGAGAATT CCTACTTAAC CGAAACTGTT CATGGAGCGA CTATGTTAAT 
GCATTTAATG AAGATCTGAG ATGGAAAGCA TCAATTAATC ATTATTTGGC TGGAGTTTTA 
AGTATCACTA ACAGCCAAAC CGAGGACAGA CGATGGAGGT ACTACTCATG TGAGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

braf ( XM_031898531.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754391.1 Xenopus tropicalis B-Raf proto-oncogene, serine/threonine kinase (braf), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa54168
Clone ID Related Accession (Same CDS sequence)	XM_031898531.1
Accession Version	XM_031898531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase B-raf isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGCTC CCGCGCCTCC GGACCACTTC AAGATGGCGG CGGTAAGTGG GGGATCAACC 
GAGGGGCCCT CACTGCTCAA CGGGGACCTG CTGGAGTCCG AGACCGGGGG ACTGGGCGGA 
GCCGGCGAGA TGTGTGCGTC CGGGGGGTAC CCGGAGGAGG TCTGGAATAT AAAGCAGATG 
ATAAAGTTAA CACAAGAGCA CATCGAAGCC CTGCTGGACA AATTTGGAGG CGAGCACAAC 
CCCCCATCTA TATACGTAGA GGCTTATGAG GAATACACCA GCAAACTGGA TCTATTACAG 
CAGAGGGAAC AGCAGTTACT GGAGTCTATG GGCAATGGAA CAGACTCTAC TTCTTTGCCT 
TCAGAGCTGA CACTTTCCTC GGTCCCATCT AATCTATCCT TGGCTCCTTC AGCTCTCTCA 
GCCCTGCGGA CCCCCAGCGA CCCCTCCCGT ATCAATCCCA AGTCTCCCCA GAAACCTATT 
GTCCGAGTCT TCCTTCCCAA CAAGCAGCGC ACTGTGGTAC CAGCTAGGAG TGGTGTGACA 
GTGCGAGACA GCCTAAAGAA AGCCCTGATG ATGCGAGGGT TAATCCCAGA ATGCTGTGCT 
GTGTACAGGG TGCAAGATGG GGAGAAGAAG CCGATTGGCT GGGATACAGA TATAAGCTGG 
ATCACTGGAG AAGAGTTACA CGTGGAAGTC TTGGAGAATG TGCCGCTCAC AACCCATAAC 
TTTGTACGGA AAACCTTCTT CACGCTGGCT TTCTGCGACT TCTGTCGGAA GCTGCTGTTC 
CAGGGCTTCC GCTGCCAGAC GTGCGGGTAC AAGTTCCACC AGCGGTGCAG CACGGAGGTG 
CCTCTTATGT GTGTTAACTA CGACCGTCTA GATTTGCTGT TTGTCTCCAA GTTCTTTGAA 
CACCACCCAA TATCTCAGGA GGAAGCTCCG TTTGAAGAGG TCGCCCCAGC CGCTGAGTCC 
TCGACTCCTC CGCCCCCTCC AGATGCCACA GGCCCCCTCT GTCTACCGAG TACTTCTCCT 
TCCAAATCCA TCCCGATTCC CCAGCCGTTC CGACCGGATG AGGACCACAG GAACCAGTTT 
GGCCAGCGAG ATCGGTCGTC CTCGGCGCCC AACGTGCACA TAAATACCAT CGAGCCGGTC 
AATATTGACG ACTTGATTCG AGACCAGGGC CTCCAGTGCA GCGAGGGAGG TTCAACCCCC 
GGCTTATCGG CGACCCCCCC TGCCTCCCTC CCCGGCTCCC TCACCAACGT CAAGGCGCTA 
CAGAAATCCC CGGGGCAGCA GAGAGAGCGC AAGTCCTCCT CGTCCTCGAC GGAAGACAGG 
AGTCGGATGA AAACGCTCGG ACGACGCGAT TCCAGCGACG ACTGGGAGAT TCCCGACGGG 
CAAATCACGG TTGGGCAGAG AATAGGTTCT GGATCGTTTG GGACAGTGTA TAAGGGAAAG 
TGGCACGGGG ACGTAGCAGT GAAAATGTTG AATGTAACGG CGCCAACGCC TCAGCAGCTA 
CAGGCCTTTA AAAATGAAGT AGGAGTCCTC AGAAAAACGC GACACGTGAA CATCCTGCTT 
TTCATGGGCT ACTCCACGAA GCCCCAGCTG CGACTAGTGA CCCAGTGGTG CGAGGGCTCC 
AGTCTCTATC ACCACCTGCA CATCATCGAG ACCAAATTCG AAATGATCAA GCTGATCGAC 
ATTGCACGAC AGACGGCGCA GGGCATGGAC TATTTGCACG CGAAGTCAAT CATCCACAGA 
GACCTAAAGA GCAATAATAT ATTCCTTCAT GAAGACCTCA CAGTAAAGAT TGGTGACTTT 
GGCCTGGCAA CAGTGAAGTC CCGGTGGAGT GGGTCTCATC AGTTTGAGCA GTTGTCTGGC 
TCCATTCTGT GGATGGCTCC TGAGGTCATT CGGATGCAAG ATAACAACCC CTATAGCTTT 
CAGTCTGATG TCTACGCGTT TGGCATTGTT CTATATGAAC TGATGACGGG GCAGTTGCCC 
TACTCCAACA TCAACAACAG AGACCAGATC ATATTTATGG TTGGGAGAGG CTACTTATCT 
CCCGACTTCA GCAAAGTTAG GAGTAATTGC CCCAAAGCAA TGAAGAGGTT AATGGCAGAC 
TGTCTAAAGA AGAAGAGAGA TGAGCGCCCC CTATTCCCAC AGATTCTCGC CTCCATCGAG 
CTGCTGGCAC GGTCATTGCC AAAAATCCAC CGCAGCGCGT CTGAGCCGTC CTTAAACCGC 
GCTGGATTCC AGACGGAAGA CTTCAGTCTG TACACGTGCG CTTCTCCCAA AACGCCTATC 
CAGGCGGGAG GTTATGAAAT CTCACCAAGA TGGGTAAATA ACTTTAATGA ACCTCTTTTT 
TATCGGTGTC CAAATCAGCA GAGTATCAAA GAAATCACAA GTATGCGTGA CAGTCAGAGG 
TGGGACCGAC TATGGGATTT TGTCTGTGAG AGATTATTCA ACTCCGTTCC TACTTGTGAA 
TGGAGCAACT ACGTCAATAA CATTGGCCAA GGATTTACCT TCTATTGCCC AACTGGCCAG 
GTTCTCAGTG GAATGGGAAA TGAACTTGAT GCCTGGGAGT CAGACAGGAG ATTGTTCCAC 
AGGTGGAAGT TTCTTTGCTG CAAAGGAGAA TTCCTACTTA ACCGAAACTG TTCATGGAGC 
GACTATGTTA ATGCATTTAA TGAAGATCTG AGATGGAAAG CATCAATTAA TCATTATTTG 
GCTGGAGTTT TAAGTATCAC TAACAGCCAA ACCGAGGACA GACGATGGAG GTACTACTCA 
TGTGAGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754391.1
CDS	91..2862
Translation	MLAPAPPDHFKMAAVSGGSTEGPSLLNGDLLESETGGLGGAGEMCASGGYPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYVEAYEEYTSKLDLLQQREQQLLESMGNGTDSTSLPSELTLSSVPSNLSLAPSALSALRTPSDPSRINPKSPQKPIVRVFLPNKQRTVVPARSGVTVRDSLKKALMMRGLIPECCAVYRVQDGEKKPIGWDTDISWITGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDRLDLLFVSKFFEHHPISQEEAPFEEVAPAAESSTPPPPPDATGPLCLPSTSPSKSIPIPQPFRPDEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGLQCSEGGSTPGLSATPPASLPGSLTNVKALQKSPGQQRERKSSSSSTEDRSRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLRLVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDNNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDFSKVRSNCPKAMKRLMADCLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYTCASPKTPIQAGGYEISPRWVNNFNEPLFYRCPNQQSIKEITSMRDSQRWDRLWDFVCERLFNSVPTCEWSNYVNNIGQGFTFYCPTGQVLSGMGNELDAWESDRRLFHRWKFLCCKGEFLLNRNCSWSDYVNAFNEDLRWKASINHYLAGVLSITNSQTEDRRWRYYSCEK

Target ORF information:

RefSeq Version	XM_031898531.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis B-Raf proto-oncogene, serine/threonine kinase (braf), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898531.1

ORF Insert Sequence:

ATGCTCGCTC CCGCGCCTCC GGACCACTTC AAGATGGCGG CGGTAAGTGG GGGATCAACC 
GAGGGGCCCT CACTGCTCAA CGGGGACCTG CTGGAGTCCG AGACCGGGGG ACTGGGCGGA 
GCCGGCGAGA TGTGTGCGTC CGGGGGGTAC CCGGAGGAGG TCTGGAATAT AAAGCAGATG 
ATAAAGTTAA CACAAGAGCA CATCGAAGCC CTGCTGGACA AATTTGGAGG CGAGCACAAC 
CCCCCATCTA TATACGTAGA GGCTTATGAG GAATACACCA GCAAACTGGA TCTATTACAG 
CAGAGGGAAC AGCAGTTACT GGAGTCTATG GGCAATGGAA CAGACTCTAC TTCTTTGCCT 
TCAGAGCTGA CACTTTCCTC GGTCCCATCT AATCTATCCT TGGCTCCTTC AGCTCTCTCA 
GCCCTGCGGA CCCCCAGCGA CCCCTCCCGT ATCAATCCCA AGTCTCCCCA GAAACCTATT 
GTCCGAGTCT TCCTTCCCAA CAAGCAGCGC ACTGTGGTAC CAGCTAGGAG TGGTGTGACA 
GTGCGAGACA GCCTAAAGAA AGCCCTGATG ATGCGAGGGT TAATCCCAGA ATGCTGTGCT 
GTGTACAGGG TGCAAGATGG GGAGAAGAAG CCGATTGGCT GGGATACAGA TATAAGCTGG 
ATCACTGGAG AAGAGTTACA CGTGGAAGTC TTGGAGAATG TGCCGCTCAC AACCCATAAC 
TTTGTACGGA AAACCTTCTT CACGCTGGCT TTCTGCGACT TCTGTCGGAA GCTGCTGTTC 
CAGGGCTTCC GCTGCCAGAC GTGCGGGTAC AAGTTCCACC AGCGGTGCAG CACGGAGGTG 
CCTCTTATGT GTGTTAACTA CGACCGTCTA GATTTGCTGT TTGTCTCCAA GTTCTTTGAA 
CACCACCCAA TATCTCAGGA GGAAGCTCCG TTTGAAGAGG TCGCCCCAGC CGCTGAGTCC 
TCGACTCCTC CGCCCCCTCC AGATGCCACA GGCCCCCTCT GTCTACCGAG TACTTCTCCT 
TCCAAATCCA TCCCGATTCC CCAGCCGTTC CGACCGGATG AGGACCACAG GAACCAGTTT 
GGCCAGCGAG ATCGGTCGTC CTCGGCGCCC AACGTGCACA TAAATACCAT CGAGCCGGTC 
AATATTGACG ACTTGATTCG AGACCAGGGC CTCCAGTGCA GCGAGGGAGG TTCAACCCCC 
GGCTTATCGG CGACCCCCCC TGCCTCCCTC CCCGGCTCCC TCACCAACGT CAAGGCGCTA 
CAGAAATCCC CGGGGCAGCA GAGAGAGCGC AAGTCCTCCT CGTCCTCGAC GGAAGACAGG 
AGTCGGATGA AAACGCTCGG ACGACGCGAT TCCAGCGACG ACTGGGAGAT TCCCGACGGG 
CAAATCACGG TTGGGCAGAG AATAGGTTCT GGATCGTTTG GGACAGTGTA TAAGGGAAAG 
TGGCACGGGG ACGTAGCAGT GAAAATGTTG AATGTAACGG CGCCAACGCC TCAGCAGCTA 
CAGGCCTTTA AAAATGAAGT AGGAGTCCTC AGAAAAACGC GACACGTGAA CATCCTGCTT 
TTCATGGGCT ACTCCACGAA GCCCCAGCTG CGACTAGTGA CCCAGTGGTG CGAGGGCTCC 
AGTCTCTATC ACCACCTGCA CATCATCGAG ACCAAATTCG AAATGATCAA GCTGATCGAC 
ATTGCACGAC AGACGGCGCA GGGCATGGAC TATTTGCACG CGAAGTCAAT CATCCACAGA 
GACCTAAAGA GCAATAATAT ATTCCTTCAT GAAGACCTCA CAGTAAAGAT TGGTGACTTT 
GGCCTGGCAA CAGTGAAGTC CCGGTGGAGT GGGTCTCATC AGTTTGAGCA GTTGTCTGGC 
TCCATTCTGT GGATGGCTCC TGAGGTCATT CGGATGCAAG ATAACAACCC CTATAGCTTT 
CAGTCTGATG TCTACGCGTT TGGCATTGTT CTATATGAAC TGATGACGGG GCAGTTGCCC 
TACTCCAACA TCAACAACAG AGACCAGATC ATATTTATGG TTGGGAGAGG CTACTTATCT 
CCCGACTTCA GCAAAGTTAG GAGTAATTGC CCCAAAGCAA TGAAGAGGTT AATGGCAGAC 
TGTCTAAAGA AGAAGAGAGA TGAGCGCCCC CTATTCCCAC AGATTCTCGC CTCCATCGAG 
CTGCTGGCAC GGTCATTGCC AAAAATCCAC CGCAGCGCGT CTGAGCCGTC CTTAAACCGC 
GCTGGATTCC AGACGGAAGA CTTCAGTCTG TACACGTGCG CTTCTCCCAA AACGCCTATC 
CAGGCGGGAG GTTATGAAAT CTCACCAAGA TGGGTAAATA ACTTTAATGA ACCTCTTTTT 
TATCGGTGTC CAAATCAGCA GAGTATCAAA GAAATCACAA GTATGCGTGA CAGTCAGAGG 
TGGGACCGAC TATGGGATTT TGTCTGTGAG AGATTATTCA ACTCCGTTCC TACTTGTGAA 
TGGAGCAACT ACGTCAATAA CATTGGCCAA GGATTTACCT TCTATTGCCC AACTGGCCAG 
GTTCTCAGTG GAATGGGAAA TGAACTTGAT GCCTGGGAGT CAGACAGGAG ATTGTTCCAC 
AGGTGGAAGT TTCTTTGCTG CAAAGGAGAA TTCCTACTTA ACCGAAACTG TTCATGGAGC 
GACTATGTTA ATGCATTTAA TGAAGATCTG AGATGGAAAG CATCAATTAA TCATTATTTG 
GCTGGAGTTT TAAGTATCAC TAACAGCCAA ACCGAGGACA GACGATGGAG GTACTACTCA 
TGTGAGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

braf ( XM_031898532.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754392.1 Xenopus tropicalis B-Raf proto-oncogene, serine/threonine kinase (braf), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa54169
Clone ID Related Accession (Same CDS sequence)	XM_031898532.1
Accession Version	XM_031898532.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase B-raf isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGCTC CCGCGCCTCC GGACCACTTC AAGATGGCGG CGGTAAGTGG GGGATCAACC 
GAGGGGCCCT CACTGCTCAA CGGGGACCTG CTGGAGTCCG AGACCGGGGG ACTGGGCGGA 
GCCGGCGAGA TGTGTGCGTC CGGGGGGTAC CCGGAGGAGG TCTGGAATAT AAAGCAGATG 
ATAAAGTTAA CACAAGAGCA CATCGAAGCC CTGCTGGACA AATTTGGAGG CGAGCACAAC 
CCCCCATCTA TATACGTAGA GGCTTATGAG GAATACACCA GCAAACTGGA TCTATTACAG 
CAGAGGGAAC AGCAGTTACT GGAGTCTATG GGCAATGGAA CAGACTCTAC TTCTTTGCCT 
TCAGAGCTGA CACTTTCCTC GGTCCCATCT AATCTATCCT TGGCTCCTTC AGCTCTCTCA 
GCCCTGCGGA CCCCCAGCGA CCCCTCCCGT ATCAATCCCA AGTCTCCCCA GAAACCTATT 
GTCCGAGTCT TCCTTCCCAA CAAGCAGCGC ACTGTGGTAC CAGCTAGGAG TGGTGTGACA 
GTGCGAGACA GCCTAAAGAA AGCCCTGATG ATGCGAGGGT TAATCCCAGA ATGCTGTGCT 
GTGTACAGGG TGCAAGATGG GGAGAAGAAG CCGATTGGCT GGGATACAGA TATAAGCTGG 
ATCACTGGAG AAGAGTTACA CGTGGAAGTC TTGGAGAATG TGCCGCTCAC AACCCATAAC 
TTTGTACGGA AAACCTTCTT CACGCTGGCT TTCTGCGACT TCTGTCGGAA GCTGCTGTTC 
CAGGGCTTCC GCTGCCAGAC GTGCGGGTAC AAGTTCCACC AGCGGTGCAG CACGGAGGTG 
CCTCTTATGT GTGTTAACTA CGACCGTCTA GATTTGCTGT TTGTCTCCAA GTTCTTTGAA 
CACCACCCAA TATCTCAGGA GGAAGCTCCG TTTGAAGAGG TCGCCCCAGC CGCTGAGTCC 
TCGACTCCTC CGCCCCCTCC AGATGCCACA GGCCCCCTCT GTCTACCGAG TACTTCTCCT 
TCCAAATCCA TCCCGATTCC CCAGCCGTTC CGACCGGATG AGGACCACAG GAACCAGTTT 
GGCCAGCGAG ATCGGTCGTC CTCGGCGCCC AACGTGCACA TAAATACCAT CGAGCCGGTC 
AATATTGACG ACTTGATTCG AGACCAGGGC CTCCAGTGCA GCGAGGGAGC CCCCTCAGTC 
CAGCCGACGC GCTGCCATCG GAAATACCAC TCGCGGGACC CCAGCCCACT CCTCCATACT 
CTCCCCAGTG AAATAGTCTT TGATTTTGAA TCTGATCCTG TTTGCAGAGG TTCAACCCCC 
GGCTTATCGG CGACCCCCCC TGCCTCCCTC CCCGGCTCCC TCACCAACGT CAAGGCGCTA 
CAGAAATCCC CGGGGCAGCA GAGAGAGCGC AAGTCCTCCT CGTCCTCGAC GGAAGACAGG 
AGTCGGATGA AAACGCTCGG ACGACGCGAT TCCAGCGACG ACTGGGAGAT TCCCGACGGG 
CAAATCACGG TTGGGCAGAG AATAGGTTCT GGATCGTTTG GGACAGTGTA TAAGGGAAAG 
TGGCACGGGG ACGTAGCAGT GAAAATGTTG AATGTAACGG CGCCAACGCC TCAGCAGCTA 
CAGGCCTTTA AAAATGAAGT AGGAGTCCTC AGAAAAACGC GACACGTGAA CATCCTGCTT 
TTCATGGGCT ACTCCACGAA GCCCCAGCTG CGACTAGTGA CCCAGTGGTG CGAGGGCTCC 
AGTCTCTATC ACCACCTGCA CATCATCGAG ACCAAATTCG AAATGATCAA GCTGATCGAC 
ATTGCACGAC AGACGGCGCA GGGCATGGAC TATTTGCACG CGAAGTCAAT CATCCACAGA 
GACCTAAAGA GCAATAATAT ATTCCTTCAT GAAGACCTCA CAGTAAAGAT TGGTGACTTT 
GGCCTGGCAA CAGTGAAGTC CCGGTGGAGT GGGTCTCATC AGTTTGAGCA GTTGTCTGGC 
TCCATTCTGT GGATGGCTCC TGAGGTCATT CGGATGCAAG ATAACAACCC CTATAGCTTT 
CAGTCTGATG TCTACGCGTT TGGCATTGTT CTATATGAAC TGATGACGGG GCAGTTGCCC 
TACTCCAACA TCAACAACAG AGACCAGATC ATATTTATGG TTGGGAGAGG CTACTTATCT 
CCCGACTTCA GCAAAGTTAG GAGTAATTGC CCCAAAGCAA TGAAGAGGTT AATGGCAGAC 
TGTCTAAAGA AGAAGAGAGA TGAGCGCCCC CTATTCCCAC AGATTCTCGC CTCCATCGAG 
CTGCTGGCAC GGTCATTGCC AAAAATCCAC CGCAGCGCGT CTGAGCCGTC CTTAAACCGC 
GCTGGATTCC AGACGGAAGA CTTCAGTCTG TACACGTGCG CTTCTCCCAA AACGCCTATC 
CAGGCGGGAG GTTATGGAGA GTTTACAGCC TTCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754392.1
CDS	85..2523
Translation	MLAPAPPDHFKMAAVSGGSTEGPSLLNGDLLESETGGLGGAGEMCASGGYPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYVEAYEEYTSKLDLLQQREQQLLESMGNGTDSTSLPSELTLSSVPSNLSLAPSALSALRTPSDPSRINPKSPQKPIVRVFLPNKQRTVVPARSGVTVRDSLKKALMMRGLIPECCAVYRVQDGEKKPIGWDTDISWITGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDRLDLLFVSKFFEHHPISQEEAPFEEVAPAAESSTPPPPPDATGPLCLPSTSPSKSIPIPQPFRPDEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGLQCSEGAPSVQPTRCHRKYHSRDPSPLLHTLPSEIVFDFESDPVCRGSTPGLSATPPASLPGSLTNVKALQKSPGQQRERKSSSSSTEDRSRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLRLVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDNNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDFSKVRSNCPKAMKRLMADCLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYTCASPKTPIQAGGYGEFTAFK

Target ORF information:

RefSeq Version	XM_031898532.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis B-Raf proto-oncogene, serine/threonine kinase (braf), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898532.1

ORF Insert Sequence:

ATGCTCGCTC CCGCGCCTCC GGACCACTTC AAGATGGCGG CGGTAAGTGG GGGATCAACC 
GAGGGGCCCT CACTGCTCAA CGGGGACCTG CTGGAGTCCG AGACCGGGGG ACTGGGCGGA 
GCCGGCGAGA TGTGTGCGTC CGGGGGGTAC CCGGAGGAGG TCTGGAATAT AAAGCAGATG 
ATAAAGTTAA CACAAGAGCA CATCGAAGCC CTGCTGGACA AATTTGGAGG CGAGCACAAC 
CCCCCATCTA TATACGTAGA GGCTTATGAG GAATACACCA GCAAACTGGA TCTATTACAG 
CAGAGGGAAC AGCAGTTACT GGAGTCTATG GGCAATGGAA CAGACTCTAC TTCTTTGCCT 
TCAGAGCTGA CACTTTCCTC GGTCCCATCT AATCTATCCT TGGCTCCTTC AGCTCTCTCA 
GCCCTGCGGA CCCCCAGCGA CCCCTCCCGT ATCAATCCCA AGTCTCCCCA GAAACCTATT 
GTCCGAGTCT TCCTTCCCAA CAAGCAGCGC ACTGTGGTAC CAGCTAGGAG TGGTGTGACA 
GTGCGAGACA GCCTAAAGAA AGCCCTGATG ATGCGAGGGT TAATCCCAGA ATGCTGTGCT 
GTGTACAGGG TGCAAGATGG GGAGAAGAAG CCGATTGGCT GGGATACAGA TATAAGCTGG 
ATCACTGGAG AAGAGTTACA CGTGGAAGTC TTGGAGAATG TGCCGCTCAC AACCCATAAC 
TTTGTACGGA AAACCTTCTT CACGCTGGCT TTCTGCGACT TCTGTCGGAA GCTGCTGTTC 
CAGGGCTTCC GCTGCCAGAC GTGCGGGTAC AAGTTCCACC AGCGGTGCAG CACGGAGGTG 
CCTCTTATGT GTGTTAACTA CGACCGTCTA GATTTGCTGT TTGTCTCCAA GTTCTTTGAA 
CACCACCCAA TATCTCAGGA GGAAGCTCCG TTTGAAGAGG TCGCCCCAGC CGCTGAGTCC 
TCGACTCCTC CGCCCCCTCC AGATGCCACA GGCCCCCTCT GTCTACCGAG TACTTCTCCT 
TCCAAATCCA TCCCGATTCC CCAGCCGTTC CGACCGGATG AGGACCACAG GAACCAGTTT 
GGCCAGCGAG ATCGGTCGTC CTCGGCGCCC AACGTGCACA TAAATACCAT CGAGCCGGTC 
AATATTGACG ACTTGATTCG AGACCAGGGC CTCCAGTGCA GCGAGGGAGC CCCCTCAGTC 
CAGCCGACGC GCTGCCATCG GAAATACCAC TCGCGGGACC CCAGCCCACT CCTCCATACT 
CTCCCCAGTG AAATAGTCTT TGATTTTGAA TCTGATCCTG TTTGCAGAGG TTCAACCCCC 
GGCTTATCGG CGACCCCCCC TGCCTCCCTC CCCGGCTCCC TCACCAACGT CAAGGCGCTA 
CAGAAATCCC CGGGGCAGCA GAGAGAGCGC AAGTCCTCCT CGTCCTCGAC GGAAGACAGG 
AGTCGGATGA AAACGCTCGG ACGACGCGAT TCCAGCGACG ACTGGGAGAT TCCCGACGGG 
CAAATCACGG TTGGGCAGAG AATAGGTTCT GGATCGTTTG GGACAGTGTA TAAGGGAAAG 
TGGCACGGGG ACGTAGCAGT GAAAATGTTG AATGTAACGG CGCCAACGCC TCAGCAGCTA 
CAGGCCTTTA AAAATGAAGT AGGAGTCCTC AGAAAAACGC GACACGTGAA CATCCTGCTT 
TTCATGGGCT ACTCCACGAA GCCCCAGCTG CGACTAGTGA CCCAGTGGTG CGAGGGCTCC 
AGTCTCTATC ACCACCTGCA CATCATCGAG ACCAAATTCG AAATGATCAA GCTGATCGAC 
ATTGCACGAC AGACGGCGCA GGGCATGGAC TATTTGCACG CGAAGTCAAT CATCCACAGA 
GACCTAAAGA GCAATAATAT ATTCCTTCAT GAAGACCTCA CAGTAAAGAT TGGTGACTTT 
GGCCTGGCAA CAGTGAAGTC CCGGTGGAGT GGGTCTCATC AGTTTGAGCA GTTGTCTGGC 
TCCATTCTGT GGATGGCTCC TGAGGTCATT CGGATGCAAG ATAACAACCC CTATAGCTTT 
CAGTCTGATG TCTACGCGTT TGGCATTGTT CTATATGAAC TGATGACGGG GCAGTTGCCC 
TACTCCAACA TCAACAACAG AGACCAGATC ATATTTATGG TTGGGAGAGG CTACTTATCT 
CCCGACTTCA GCAAAGTTAG GAGTAATTGC CCCAAAGCAA TGAAGAGGTT AATGGCAGAC 
TGTCTAAAGA AGAAGAGAGA TGAGCGCCCC CTATTCCCAC AGATTCTCGC CTCCATCGAG 
CTGCTGGCAC GGTCATTGCC AAAAATCCAC CGCAGCGCGT CTGAGCCGTC CTTAAACCGC 
GCTGGATTCC AGACGGAAGA CTTCAGTCTG TACACGTGCG CTTCTCCCAA AACGCCTATC 
CAGGCGGGAG GTTATGGAGA GTTTACAGCC TTCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

braf ( XM_031898533.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754393.1 Xenopus tropicalis B-Raf proto-oncogene, serine/threonine kinase (braf), transcript variant X6, mRNA.

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CloneID	OXa54170
Clone ID Related Accession (Same CDS sequence)	XM_031898533.1
Accession Version	XM_031898533.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase B-raf isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGCTC CCGCGCCTCC GGACCACTTC AAGATGGCGG CGGTAAGTGG GGGATCAACC 
GAGGGGCCCT CACTGCTCAA CGGGGACCTG CTGGAGTCCG AGACCGGGGG ACTGGGCGGA 
GCCGGCGAGA TGTGTGCGTC CGGGGGGTAC CCGGAGGAGG TCTGGAATAT AAAGCAGATG 
ATAAAGTTAA CACAAGAGCA CATCGAAGCC CTGCTGGACA AATTTGGAGG CGAGCACAAC 
CCCCCATCTA TATACGTAGA GGCTTATGAG GAATACACCA GCAAACTGGA TCTATTACAG 
CAGAGGGAAC AGCAGTTACT GGAGTCTATG GGCAATGGAA CAGACTCTAC TTCTTTGCCT 
TCAGAGCTGA CACTTTCCTC GGTCCCATCT AATCTATCCT TGGCTCCTTC AGCTCTCTCA 
GCCCTGCGGA CCCCCAGCGA CCCCTCCCGT ATCAATCCCA AGTCTCCCCA GAAACCTATT 
GTCCGAGTCT TCCTTCCCAA CAAGCAGCGC ACTGTGGTAC CAGCTAGGAG TGGTGTGACA 
GTGCGAGACA GCCTAAAGAA AGCCCTGATG ATGCGAGGGT TAATCCCAGA ATGCTGTGCT 
GTGTACAGGG TGCAAGATGG GGAGAAGAAG CCGATTGGCT GGGATACAGA TATAAGCTGG 
ATCACTGGAG AAGAGTTACA CGTGGAAGTC TTGGAGAATG TGCCGCTCAC AACCCATAAC 
TTTGTACGGA AAACCTTCTT CACGCTGGCT TTCTGCGACT TCTGTCGGAA GCTGCTGTTC 
CAGGGCTTCC GCTGCCAGAC GTGCGGGTAC AAGTTCCACC AGCGGTGCAG CACGGAGGTG 
CCTCTTATGT GTGTTAACTA CGACCGTCTA GATTTGCTGT TTGTCTCCAA GTTCTTTGAA 
CACCACCCAA TATCTCAGGA GGAAGCTCCG TTTGAAGAGG TCGCCCCAGC CGCTGAGTCC 
TCGACTCCTC CGCCCCCTCC AGATGCCACA GGCCCCCTCT GTCTACCGAG TACTTCTCCT 
TCCAAATCCA TCCCGATTCC CCAGCCGTTC CGACCGGATG AGGACCACAG GAACCAGTTT 
GGCCAGCGAG ATCGGTCGTC CTCGGCGCCC AACGTGCACA TAAATACCAT CGAGCCGGTC 
AATATTGACG ACTTGATTCG AGACCAGGGC CTCCAGTGCA GCGAGGGAGG TTCAACCCCC 
GGCTTATCGG CGACCCCCCC TGCCTCCCTC CCCGGCTCCC TCACCAACGT CAAGGCGCTA 
CAGAAATCCC CGGGGCAGCA GAGAGAGCGC AAGTCCTCCT CGTCCTCGAC GGAAGACAGG 
AGTCGGATGA AAACGCTCGG ACGACGCGAT TCCAGCGACG ACTGGGAGAT TCCCGACGGG 
CAAATCACGG TTGGGCAGAG AATAGGTTCT GGATCGTTTG GGACAGTGTA TAAGGGAAAG 
TGGCACGGGG ACGTAGCAGT GAAAATGTTG AATGTAACGG CGCCAACGCC TCAGCAGCTA 
CAGGCCTTTA AAAATGAAGT AGGAGTCCTC AGAAAAACGC GACACGTGAA CATCCTGCTT 
TTCATGGGCT ACTCCACGAA GCCCCAGCTG CGACTAGTGA CCCAGTGGTG CGAGGGCTCC 
AGTCTCTATC ACCACCTGCA CATCATCGAG ACCAAATTCG AAATGATCAA GCTGATCGAC 
ATTGCACGAC AGACGGCGCA GGGCATGGAC TATTTGCACG CGAAGTCAAT CATCCACAGA 
GACCTAAAGA GCAATAATAT ATTCCTTCAT GAAGACCTCA CAGTAAAGAT TGGTGACTTT 
GGCCTGGCAA CAGTGAAGTC CCGGTGGAGT GGGTCTCATC AGTTTGAGCA GTTGTCTGGC 
TCCATTCTGT GGATGGCTCC TGAGGTCATT CGGATGCAAG ATAACAACCC CTATAGCTTT 
CAGTCTGATG TCTACGCGTT TGGCATTGTT CTATATGAAC TGATGACGGG GCAGTTGCCC 
TACTCCAACA TCAACAACAG AGACCAGATC ATATTTATGG TTGGGAGAGG CTACTTATCT 
CCCGACTTCA GCAAAGTTAG GAGTAATTGC CCCAAAGCAA TGAAGAGGTT AATGGCAGAC 
TGTCTAAAGA AGAAGAGAGA TGAGCGCCCC CTATTCCCAC AGATTCTCGC CTCCATCGAG 
CTGCTGGCAC GGTCATTGCC AAAAATCCAC CGCAGCGCGT CTGAGCCGTC CTTAAACCGC 
GCTGGATTCC AGACGGAAGA CTTCAGTCTG TACACGTGCG CTTCTCCCAA AACGCCTATC 
CAGGCGGGAG GTTATGGAGA GTTTACAGCC TTCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754393.1
CDS	86..2404
Translation	MLAPAPPDHFKMAAVSGGSTEGPSLLNGDLLESETGGLGGAGEMCASGGYPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYVEAYEEYTSKLDLLQQREQQLLESMGNGTDSTSLPSELTLSSVPSNLSLAPSALSALRTPSDPSRINPKSPQKPIVRVFLPNKQRTVVPARSGVTVRDSLKKALMMRGLIPECCAVYRVQDGEKKPIGWDTDISWITGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDRLDLLFVSKFFEHHPISQEEAPFEEVAPAAESSTPPPPPDATGPLCLPSTSPSKSIPIPQPFRPDEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGLQCSEGGSTPGLSATPPASLPGSLTNVKALQKSPGQQRERKSSSSSTEDRSRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLRLVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDNNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDFSKVRSNCPKAMKRLMADCLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYTCASPKTPIQAGGYGEFTAFK

Target ORF information:

RefSeq Version	XM_031898533.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis B-Raf proto-oncogene, serine/threonine kinase (braf), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898533.1

ORF Insert Sequence:

ATGCTCGCTC CCGCGCCTCC GGACCACTTC AAGATGGCGG CGGTAAGTGG GGGATCAACC 
GAGGGGCCCT CACTGCTCAA CGGGGACCTG CTGGAGTCCG AGACCGGGGG ACTGGGCGGA 
GCCGGCGAGA TGTGTGCGTC CGGGGGGTAC CCGGAGGAGG TCTGGAATAT AAAGCAGATG 
ATAAAGTTAA CACAAGAGCA CATCGAAGCC CTGCTGGACA AATTTGGAGG CGAGCACAAC 
CCCCCATCTA TATACGTAGA GGCTTATGAG GAATACACCA GCAAACTGGA TCTATTACAG 
CAGAGGGAAC AGCAGTTACT GGAGTCTATG GGCAATGGAA CAGACTCTAC TTCTTTGCCT 
TCAGAGCTGA CACTTTCCTC GGTCCCATCT AATCTATCCT TGGCTCCTTC AGCTCTCTCA 
GCCCTGCGGA CCCCCAGCGA CCCCTCCCGT ATCAATCCCA AGTCTCCCCA GAAACCTATT 
GTCCGAGTCT TCCTTCCCAA CAAGCAGCGC ACTGTGGTAC CAGCTAGGAG TGGTGTGACA 
GTGCGAGACA GCCTAAAGAA AGCCCTGATG ATGCGAGGGT TAATCCCAGA ATGCTGTGCT 
GTGTACAGGG TGCAAGATGG GGAGAAGAAG CCGATTGGCT GGGATACAGA TATAAGCTGG 
ATCACTGGAG AAGAGTTACA CGTGGAAGTC TTGGAGAATG TGCCGCTCAC AACCCATAAC 
TTTGTACGGA AAACCTTCTT CACGCTGGCT TTCTGCGACT TCTGTCGGAA GCTGCTGTTC 
CAGGGCTTCC GCTGCCAGAC GTGCGGGTAC AAGTTCCACC AGCGGTGCAG CACGGAGGTG 
CCTCTTATGT GTGTTAACTA CGACCGTCTA GATTTGCTGT TTGTCTCCAA GTTCTTTGAA 
CACCACCCAA TATCTCAGGA GGAAGCTCCG TTTGAAGAGG TCGCCCCAGC CGCTGAGTCC 
TCGACTCCTC CGCCCCCTCC AGATGCCACA GGCCCCCTCT GTCTACCGAG TACTTCTCCT 
TCCAAATCCA TCCCGATTCC CCAGCCGTTC CGACCGGATG AGGACCACAG GAACCAGTTT 
GGCCAGCGAG ATCGGTCGTC CTCGGCGCCC AACGTGCACA TAAATACCAT CGAGCCGGTC 
AATATTGACG ACTTGATTCG AGACCAGGGC CTCCAGTGCA GCGAGGGAGG TTCAACCCCC 
GGCTTATCGG CGACCCCCCC TGCCTCCCTC CCCGGCTCCC TCACCAACGT CAAGGCGCTA 
CAGAAATCCC CGGGGCAGCA GAGAGAGCGC AAGTCCTCCT CGTCCTCGAC GGAAGACAGG 
AGTCGGATGA AAACGCTCGG ACGACGCGAT TCCAGCGACG ACTGGGAGAT TCCCGACGGG 
CAAATCACGG TTGGGCAGAG AATAGGTTCT GGATCGTTTG GGACAGTGTA TAAGGGAAAG 
TGGCACGGGG ACGTAGCAGT GAAAATGTTG AATGTAACGG CGCCAACGCC TCAGCAGCTA 
CAGGCCTTTA AAAATGAAGT AGGAGTCCTC AGAAAAACGC GACACGTGAA CATCCTGCTT 
TTCATGGGCT ACTCCACGAA GCCCCAGCTG CGACTAGTGA CCCAGTGGTG CGAGGGCTCC 
AGTCTCTATC ACCACCTGCA CATCATCGAG ACCAAATTCG AAATGATCAA GCTGATCGAC 
ATTGCACGAC AGACGGCGCA GGGCATGGAC TATTTGCACG CGAAGTCAAT CATCCACAGA 
GACCTAAAGA GCAATAATAT ATTCCTTCAT GAAGACCTCA CAGTAAAGAT TGGTGACTTT 
GGCCTGGCAA CAGTGAAGTC CCGGTGGAGT GGGTCTCATC AGTTTGAGCA GTTGTCTGGC 
TCCATTCTGT GGATGGCTCC TGAGGTCATT CGGATGCAAG ATAACAACCC CTATAGCTTT 
CAGTCTGATG TCTACGCGTT TGGCATTGTT CTATATGAAC TGATGACGGG GCAGTTGCCC 
TACTCCAACA TCAACAACAG AGACCAGATC ATATTTATGG TTGGGAGAGG CTACTTATCT 
CCCGACTTCA GCAAAGTTAG GAGTAATTGC CCCAAAGCAA TGAAGAGGTT AATGGCAGAC 
TGTCTAAAGA AGAAGAGAGA TGAGCGCCCC CTATTCCCAC AGATTCTCGC CTCCATCGAG 
CTGCTGGCAC GGTCATTGCC AAAAATCCAC CGCAGCGCGT CTGAGCCGTC CTTAAACCGC 
GCTGGATTCC AGACGGAAGA CTTCAGTCTG TACACGTGCG CTTCTCCCAA AACGCCTATC 
CAGGCGGGAG GTTATGGAGA GTTTACAGCC TTCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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braf cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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