znf654 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following znf654 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the znf654 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa06327 XM_004912076.4
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Xenopus tropicalis zinc finger protein 654 (znf654), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
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OXa06327 XM_004912076.3 Xenopus tropicalis zinc finger protein 654 (znf654), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa06327
    Clone ID Related Accession (Same CDS sequence) XM_004912076.3 , XM_004912076.4
    Accession Version XM_004912076.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3315bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product zinc finger protein 654
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912076.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCGGAAG ACGAGAGCGA TCAAGAATCG GAGAGACTGG TAGAAGAGCT GGTAGCGATC 
    GCGGATGAAG ACGTGGGGAA TGGCGACTTG TGCCGTGTGA ACAGCAGGGA ATACTGCCGG
    CGCTTTTGCG AGGTTGTTGA AGATTATACT TCAAGATGCC AGGTTCCTTT ACCCCAACTC
    CAAGTCCTCC AAACAGCTCT GTGTTGCTTC ACATCTGCCA CCATATCCTT CCCAGCCGAA
    TGTGAGCACG TACAGTATGT TTTGAGTAGA CTAGCTTTGA GTCTGTTTGA ATTGTTACTC
    TTCTTTGGAA AAGATGAGTT TTACGAGGCT CCTCTGAAAG ACATTCTTGG CTCGGTGCAG
    GAGTGCCACG ACCACCTGAT CCGATATGAT AATACAGATT TAAAGCTGGT CACTTACGTC
    ATTAAGGATG GCGGGCCTTG GGAGAATCCA GTATTACAAG CTATACTCAA GGGAAGATCG
    GAACCACAGG ATATAGTCGA CAGGTATTTG AGGTCTGAGA GTGAGTTCTT CTTTGAGTTG
    CGAGTGAGGT ACCTCATTGC CTGTGAGCGT ATCCCAGAAG CAGTGGCTCT CATCACAACT
    TGCCTAAGTA ACAGCGATGT CAGTAAAAAC CTGTACTACC ATCAAGCGTA CTTCATATGC
    CTACATATGA CCCGATTAAC TGACCAGCTT CTGCACGAGC ATGTTCTAAG GATAAATGCA
    AGAGATGGGG TAGAAATTAT CTGCAGCACT GAGCTGGAAG GTAAAACGGT ACTGGCGTTG
    CAGCTGAGCG AAGCTTTCCT CCTCACACAG CTACAGACTG GGGAGATGGA CTGCATTTGG
    GATTTAATAT TTATTTGGAG CAAGCTTCAG CTGAAAATGA ACTCCTCGAA GCAAGCCTTT
    GTTGAGCAGT GCTATCAGAT GTTAAGGATA GCCACCAACA TTAAAGTCAT CTTCCCTTTC
    ATAAAAACAA TTTTAAATGA AGTTGGAGAA GTCGCTGGGC AGTTGGCTGT AGAGCTTTGT
    GGATGTGCAT TACAATTAGA CCTTCATGAT AACCCAGAAA CCAAATCATT AATTTATAAA
    ACAATTGCTT ACCTTTTGCC CACTGACTTG GAGATTTGCA GGGTCTGCGC TTTATCCGTT
    TTCTTCTTGG AGAGGACCGC CGAATCCTAT GCTGCAGTCG AACGCTTGTA CAAATGTTCC
    GACGAGGATT ACAATGAATT TTTGAGCTGT GTTGAAAACC GGGTGCGTTT TGAATTGCTT
    CCCATTCTGA AAAGGGGGTT AATGTTTGAT CCAGAGTTTT GGAATTTCTC TATGATCGAG
    GAAAAATGTA CTTCTCTCCT TGGGGAAGAT GAGCCTCCAG CATCATGTAA TGCAAGTACA
    CTGGATACTG AAAATTCAGA TACCTCTAAT ATGGACTTGA AAAATGGGGT CATTGACAAT
    GGAGAGTCCA GGAGCACCAG ATCAAAAATC TCTCTTGTTA AAAAGAGTAT TCCTCCTATG
    CTTGGCTCTT ACAAGATAGA TCATAATGTT CCCGTTCATC AGTGTACCCT GTGCAACAAG
    GTGATCCTTG GTGGCCATAT TTTCAGACAT GCTCAGGCTC ATCAAAAAAA AGGCTGCTTT
    TCGTGTGTTT TATGCTCCAG GAAGTTCAGA AATCGCGTTA AAATCCTTAA ACATTTAAAG
    AACCACATAA AGAAAATGCA GGTACAGCCA ATGGCTGCAC TTGACGCTGC TTCTGTTGGA
    TTAACAGACT CTGCCTCAAG TGGAGATAAA GAAACATCGT GCTTGGAAAA TGGAAATTCT
    AGTAGTTCCA CCACTGATGT TTTAACTGTT GCCCCAGCCG TAAATATGAC TTATCCAGAA
    TCCATTCATC CCATAATTCC TGTTGAAAAT CACTCAGCTG ATCATATTAA TCAGCCTGAC
    GTAAATGATT CAGAGGACGC AGTTGATGTC ACGTCCAGTG CTAACGATGC CTCTGACTGC
    ATTAAAATAA ATGGGTCTGT TTGCCCTCTT GCAGCAAAGC AGGCTGTAAA GGAGCAAGGT
    GTTTATAAAT GCCCGGCTCA AGGGTGTATA CGTCTTTTTA CAAAGATGAG GTCTCTTAAC
    AAGCATGCCA GGATAGCCCA CCCAGCGGAT GCGAGAGTGC AGCAACACTT AATGAAACAC
    AACAAGGGAA AATGCAGATT CTGTCAGAGA AGGTTTGCCG ATTCCAAACA CTTTTTTGAT
    CACATGAAGA GGCACATTTA TCCCGAGGTC TATTTCTGCC AGCATTTAGA TTGCAGTAAA
    AGGTTCAAGC TAGTGACCCA TCTGGCAGAG CACCTGCTAA GCCATGAATC ATTCCAAGCT
    CAGTGTAGCT TTGTTAACTG TTTTGAGATT TTTGATAAAC TCTCCTCACT TTATGAGCAT
    GAAGCTGTGC ACTATAGTCA GAATCCCTGT GAAGCTGCCA CTCCATATCC TGATGTGGAG
    ATCATGGCCG GTGTTGCTGC TTCCAGTGCT TCTGTAGGCA ATGCTCATTT AGATCAGGTT
    CCTTCAGATG GTTGTACAAA GGACCATATT CTTGCAGAGA ATTCTTCCTT GGGGGAATTG
    CACGACTTGA TGGACAATTA CAAGCACTTC TCTAGGCTTT CTAAAGGAAA GAAACCTGAG
    GACAACACGG AGGTAGCAGA CTTACCAGTA CCTGTTTGGA AAGCAAGAAA GGACACAGTT
    GAACCAAAGA CCTATACACA GACAGAGAAA AAAGTAAATG GGGACATCCA GAACGTTAAT
    AGAAATTCAC AAGAAAGTTC AAATCTGCAA GAGATTGAGC AGCCACAGCC TCCTGAGCAA
    GGAAACTTGG CACAAAGTGA CATTGCCGAT GGAAGTAGTC TGAATAAATG TAACGTGCAG
    GAGCAGACTG TAGTGCAAAC ACCTTGCTTG TTGGATACCG CACCACCTAA TACGGAGACT
    GTTGCAAAAG AGGGTTTGGT TATGGATGAC ATGCAGGACG TCTTTGAAGA AAAAGGTGCT
    GCTGGGTTTG CTTGCTCCAA AAGGCCTCCA GGCTCTCCTC TTACAACATA TGGTAGGGCT
    CCTAAGGCGC CATTTATCAG ACCTCAGCCT CCAAGTTACC TAGATGAACA GTATATCAGT
    ATGCCTAAGC GGAGAAAAAA TGCTGAGGAA CTGACGCATG AAAACGACAA AGTTTCTAAA
    AAATCATCAG AAAGATTCAG ATGTGGGAAG TGCCTAACTA ATTACTCTAG CTCAGAAGCA
    CTTAATGAGC ACACAGCACA AAACAAATGT CAGCTTTATT TTGGGTTTGA CTCCGATGAT
    GAAAGTGCCT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004912133.1
    CDS130..3444
    Translation

    Target ORF information:

    RefSeq Version XM_004912076.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis zinc finger protein 654 (znf654), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004912076.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCGGAAG ACGAGAGCGA TCAAGAATCG GAGAGACTGG TAGAAGAGCT GGTAGCGATC 
    GCGGATGAAG ACGTGGGGAA TGGCGACTTG TGCCGTGTGA ACAGCAGGGA ATACTGCCGG
    CGCTTTTGCG AGGTTGTTGA AGATTATACT TCAAGATGCC AGGTTCCTTT ACCCCAACTC
    CAAGTCCTCC AAACAGCTCT GTGTTGCTTC ACATCTGCCA CCATATCCTT CCCAGCCGAA
    TGTGAGCACG TACAGTATGT TTTGAGTAGA CTAGCTTTGA GTCTGTTTGA ATTGTTACTC
    TTCTTTGGAA AAGATGAGTT TTACGAGGCT CCTCTGAAAG ACATTCTTGG CTCGGTGCAG
    GAGTGCCACG ACCACCTGAT CCGATATGAT AATACAGATT TAAAGCTGGT CACTTACGTC
    ATTAAGGATG GCGGGCCTTG GGAGAATCCA GTATTACAAG CTATACTCAA GGGAAGATCG
    GAACCACAGG ATATAGTCGA CAGGTATTTG AGGTCTGAGA GTGAGTTCTT CTTTGAGTTG
    CGAGTGAGGT ACCTCATTGC CTGTGAGCGT ATCCCAGAAG CAGTGGCTCT CATCACAACT
    TGCCTAAGTA ACAGCGATGT CAGTAAAAAC CTGTACTACC ATCAAGCGTA CTTCATATGC
    CTACATATGA CCCGATTAAC TGACCAGCTT CTGCACGAGC ATGTTCTAAG GATAAATGCA
    AGAGATGGGG TAGAAATTAT CTGCAGCACT GAGCTGGAAG GTAAAACGGT ACTGGCGTTG
    CAGCTGAGCG AAGCTTTCCT CCTCACACAG CTACAGACTG GGGAGATGGA CTGCATTTGG
    GATTTAATAT TTATTTGGAG CAAGCTTCAG CTGAAAATGA ACTCCTCGAA GCAAGCCTTT
    GTTGAGCAGT GCTATCAGAT GTTAAGGATA GCCACCAACA TTAAAGTCAT CTTCCCTTTC
    ATAAAAACAA TTTTAAATGA AGTTGGAGAA GTCGCTGGGC AGTTGGCTGT AGAGCTTTGT
    GGATGTGCAT TACAATTAGA CCTTCATGAT AACCCAGAAA CCAAATCATT AATTTATAAA
    ACAATTGCTT ACCTTTTGCC CACTGACTTG GAGATTTGCA GGGTCTGCGC TTTATCCGTT
    TTCTTCTTGG AGAGGACCGC CGAATCCTAT GCTGCAGTCG AACGCTTGTA CAAATGTTCC
    GACGAGGATT ACAATGAATT TTTGAGCTGT GTTGAAAACC GGGTGCGTTT TGAATTGCTT
    CCCATTCTGA AAAGGGGGTT AATGTTTGAT CCAGAGTTTT GGAATTTCTC TATGATCGAG
    GAAAAATGTA CTTCTCTCCT TGGGGAAGAT GAGCCTCCAG CATCATGTAA TGCAAGTACA
    CTGGATACTG AAAATTCAGA TACCTCTAAT ATGGACTTGA AAAATGGGGT CATTGACAAT
    GGAGAGTCCA GGAGCACCAG ATCAAAAATC TCTCTTGTTA AAAAGAGTAT TCCTCCTATG
    CTTGGCTCTT ACAAGATAGA TCATAATGTT CCCGTTCATC AGTGTACCCT GTGCAACAAG
    GTGATCCTTG GTGGCCATAT TTTCAGACAT GCTCAGGCTC ATCAAAAAAA AGGCTGCTTT
    TCGTGTGTTT TATGCTCCAG GAAGTTCAGA AATCGCGTTA AAATCCTTAA ACATTTAAAG
    AACCACATAA AGAAAATGCA GGTACAGCCA ATGGCTGCAC TTGACGCTGC TTCTGTTGGA
    TTAACAGACT CTGCCTCAAG TGGAGATAAA GAAACATCGT GCTTGGAAAA TGGAAATTCT
    AGTAGTTCCA CCACTGATGT TTTAACTGTT GCCCCAGCCG TAAATATGAC TTATCCAGAA
    TCCATTCATC CCATAATTCC TGTTGAAAAT CACTCAGCTG ATCATATTAA TCAGCCTGAC
    GTAAATGATT CAGAGGACGC AGTTGATGTC ACGTCCAGTG CTAACGATGC CTCTGACTGC
    ATTAAAATAA ATGGGTCTGT TTGCCCTCTT GCAGCAAAGC AGGCTGTAAA GGAGCAAGGT
    GTTTATAAAT GCCCGGCTCA AGGGTGTATA CGTCTTTTTA CAAAGATGAG GTCTCTTAAC
    AAGCATGCCA GGATAGCCCA CCCAGCGGAT GCGAGAGTGC AGCAACACTT AATGAAACAC
    AACAAGGGAA AATGCAGATT CTGTCAGAGA AGGTTTGCCG ATTCCAAACA CTTTTTTGAT
    CACATGAAGA GGCACATTTA TCCCGAGGTC TATTTCTGCC AGCATTTAGA TTGCAGTAAA
    AGGTTCAAGC TAGTGACCCA TCTGGCAGAG CACCTGCTAA GCCATGAATC ATTCCAAGCT
    CAGTGTAGCT TTGTTAACTG TTTTGAGATT TTTGATAAAC TCTCCTCACT TTATGAGCAT
    GAAGCTGTGC ACTATAGTCA GAATCCCTGT GAAGCTGCCA CTCCATATCC TGATGTGGAG
    ATCATGGCCG GTGTTGCTGC TTCCAGTGCT TCTGTAGGCA ATGCTCATTT AGATCAGGTT
    CCTTCAGATG GTTGTACAAA GGACCATATT CTTGCAGAGA ATTCTTCCTT GGGGGAATTG
    CACGACTTGA TGGACAATTA CAAGCACTTC TCTAGGCTTT CTAAAGGAAA GAAACCTGAG
    GACAACACGG AGGTAGCAGA CTTACCAGTA CCTGTTTGGA AAGCAAGAAA GGACACAGTT
    GAACCAAAGA CCTATACACA GACAGAGAAA AAAGTAAATG GGGACATCCA GAACGTTAAT
    AGAAATTCAC AAGAAAGTTC AAATCTGCAA GAGATTGAGC AGCCACAGCC TCCTGAGCAA
    GGAAACTTGG CACAAAGTGA CATTGCCGAT GGAAGTAGTC TGAATAAATG TAACGTGCAG
    GAGCAGACTG TAGTGCAAAC ACCTTGCTTG TTGGATACCG CACCACCTAA TACGGAGACT
    GTTGCAAAAG AGGGTTTGGT TATGGATGAC ATGCAGGACG TCTTTGAAGA AAAAGGTGCT
    GCTGGGTTTG CTTGCTCCAA AAGGCCTCCA GGCTCTCCTC TTACAACATA TGGTAGGGCT
    CCTAAGGCGC CATTTATCAG ACCTCAGCCT CCAAGTTACC TAGATGAACA GTATATCAGT
    ATGCCTAAGC GGAGAAAAAA TGCTGAGGAA CTGACGCATG AAAACGACAA AGTTTCTAAA
    AAATCATCAG AAAGATTCAG ATGTGGGAAG TGCCTAACTA ATTACTCTAG CTCAGAAGCA
    CTTAATGAGC ACACAGCACA AAACAAATGT CAGCTTTATT TTGGGTTTGA CTCCGATGAT
    GAAAGTGCCT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06327
    Clone ID Related Accession (Same CDS sequence) XM_004912076.3 , XM_004912076.4
    Accession Version XM_004912076.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3315bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product zinc finger protein 654
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912076.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCGGAAG ACGAGAGCGA TCAAGAATCG GAGAGACTGG TAGAAGAGCT GGTAGCGATC 
    GCGGATGAAG ACGTGGGGAA TGGCGACTTG TGCCGTGTGA ACAGCAGGGA ATACTGCCGG
    CGCTTTTGCG AGGTTGTTGA AGATTATACT TCAAGATGCC AGGTTCCTTT ACCCCAACTC
    CAAGTCCTCC AAACAGCTCT GTGTTGCTTC ACATCTGCCA CCATATCCTT CCCAGCCGAA
    TGTGAGCACG TACAGTATGT TTTGAGTAGA CTAGCTTTGA GTCTGTTTGA ATTGTTACTC
    TTCTTTGGAA AAGATGAGTT TTACGAGGCT CCTCTGAAAG ACATTCTTGG CTCGGTGCAG
    GAGTGCCACG ACCACCTGAT CCGATATGAT AATACAGATT TAAAGCTGGT CACTTACGTC
    ATTAAGGATG GCGGGCCTTG GGAGAATCCA GTATTACAAG CTATACTCAA GGGAAGATCG
    GAACCACAGG ATATAGTCGA CAGGTATTTG AGGTCTGAGA GTGAGTTCTT CTTTGAGTTG
    CGAGTGAGGT ACCTCATTGC CTGTGAGCGT ATCCCAGAAG CAGTGGCTCT CATCACAACT
    TGCCTAAGTA ACAGCGATGT CAGTAAAAAC CTGTACTACC ATCAAGCGTA CTTCATATGC
    CTACATATGA CCCGATTAAC TGACCAGCTT CTGCACGAGC ATGTTCTAAG GATAAATGCA
    AGAGATGGGG TAGAAATTAT CTGCAGCACT GAGCTGGAAG GTAAAACGGT ACTGGCGTTG
    CAGCTGAGCG AAGCTTTCCT CCTCACACAG CTACAGACTG GGGAGATGGA CTGCATTTGG
    GATTTAATAT TTATTTGGAG CAAGCTTCAG CTGAAAATGA ACTCCTCGAA GCAAGCCTTT
    GTTGAGCAGT GCTATCAGAT GTTAAGGATA GCCACCAACA TTAAAGTCAT CTTCCCTTTC
    ATAAAAACAA TTTTAAATGA AGTTGGAGAA GTCGCTGGGC AGTTGGCTGT AGAGCTTTGT
    GGATGTGCAT TACAATTAGA CCTTCATGAT AACCCAGAAA CCAAATCATT AATTTATAAA
    ACAATTGCTT ACCTTTTGCC CACTGACTTG GAGATTTGCA GGGTCTGCGC TTTATCCGTT
    TTCTTCTTGG AGAGGACCGC CGAATCCTAT GCTGCAGTCG AACGCTTGTA CAAATGTTCC
    GACGAGGATT ACAATGAATT TTTGAGCTGT GTTGAAAACC GGGTGCGTTT TGAATTGCTT
    CCCATTCTGA AAAGGGGGTT AATGTTTGAT CCAGAGTTTT GGAATTTCTC TATGATCGAG
    GAAAAATGTA CTTCTCTCCT TGGGGAAGAT GAGCCTCCAG CATCATGTAA TGCAAGTACA
    CTGGATACTG AAAATTCAGA TACCTCTAAT ATGGACTTGA AAAATGGGGT CATTGACAAT
    GGAGAGTCCA GGAGCACCAG ATCAAAAATC TCTCTTGTTA AAAAGAGTAT TCCTCCTATG
    CTTGGCTCTT ACAAGATAGA TCATAATGTT CCCGTTCATC AGTGTACCCT GTGCAACAAG
    GTGATCCTTG GTGGCCATAT TTTCAGACAT GCTCAGGCTC ATCAAAAAAA AGGCTGCTTT
    TCGTGTGTTT TATGCTCCAG GAAGTTCAGA AATCGCGTTA AAATCCTTAA ACATTTAAAG
    AACCACATAA AGAAAATGCA GGTACAGCCA ATGGCTGCAC TTGACGCTGC TTCTGTTGGA
    TTAACAGACT CTGCCTCAAG TGGAGATAAA GAAACATCGT GCTTGGAAAA TGGAAATTCT
    AGTAGTTCCA CCACTGATGT TTTAACTGTT GCCCCAGCCG TAAATATGAC TTATCCAGAA
    TCCATTCATC CCATAATTCC TGTTGAAAAT CACTCAGCTG ATCATATTAA TCAGCCTGAC
    GTAAATGATT CAGAGGACGC AGTTGATGTC ACGTCCAGTG CTAACGATGC CTCTGACTGC
    ATTAAAATAA ATGGGTCTGT TTGCCCTCTT GCAGCAAAGC AGGCTGTAAA GGAGCAAGGT
    GTTTATAAAT GCCCGGCTCA AGGGTGTATA CGTCTTTTTA CAAAGATGAG GTCTCTTAAC
    AAGCATGCCA GGATAGCCCA CCCAGCGGAT GCGAGAGTGC AGCAACACTT AATGAAACAC
    AACAAGGGAA AATGCAGATT CTGTCAGAGA AGGTTTGCCG ATTCCAAACA CTTTTTTGAT
    CACATGAAGA GGCACATTTA TCCCGAGGTC TATTTCTGCC AGCATTTAGA TTGCAGTAAA
    AGGTTCAAGC TAGTGACCCA TCTGGCAGAG CACCTGCTAA GCCATGAATC ATTCCAAGCT
    CAGTGTAGCT TTGTTAACTG TTTTGAGATT TTTGATAAAC TCTCCTCACT TTATGAGCAT
    GAAGCTGTGC ACTATAGTCA GAATCCCTGT GAAGCTGCCA CTCCATATCC TGATGTGGAG
    ATCATGGCCG GTGTTGCTGC TTCCAGTGCT TCTGTAGGCA ATGCTCATTT AGATCAGGTT
    CCTTCAGATG GTTGTACAAA GGACCATATT CTTGCAGAGA ATTCTTCCTT GGGGGAATTG
    CACGACTTGA TGGACAATTA CAAGCACTTC TCTAGGCTTT CTAAAGGAAA GAAACCTGAG
    GACAACACGG AGGTAGCAGA CTTACCAGTA CCTGTTTGGA AAGCAAGAAA GGACACAGTT
    GAACCAAAGA CCTATACACA GACAGAGAAA AAAGTAAATG GGGACATCCA GAACGTTAAT
    AGAAATTCAC AAGAAAGTTC AAATCTGCAA GAGATTGAGC AGCCACAGCC TCCTGAGCAA
    GGAAACTTGG CACAAAGTGA CATTGCCGAT GGAAGTAGTC TGAATAAATG TAACGTGCAG
    GAGCAGACTG TAGTGCAAAC ACCTTGCTTG TTGGATACCG CACCACCTAA TACGGAGACT
    GTTGCAAAAG AGGGTTTGGT TATGGATGAC ATGCAGGACG TCTTTGAAGA AAAAGGTGCT
    GCTGGGTTTG CTTGCTCCAA AAGGCCTCCA GGCTCTCCTC TTACAACATA TGGTAGGGCT
    CCTAAGGCGC CATTTATCAG ACCTCAGCCT CCAAGTTACC TAGATGAACA GTATATCAGT
    ATGCCTAAGC GGAGAAAAAA TGCTGAGGAA CTGACGCATG AAAACGACAA AGTTTCTAAA
    AAATCATCAG AAAGATTCAG ATGTGGGAAG TGCCTAACTA ATTACTCTAG CTCAGAAGCA
    CTTAATGAGC ACACAGCACA AAACAAATGT CAGCTTTATT TTGGGTTTGA CTCCGATGAT
    GAAAGTGCCT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004912133.1
    CDS128..3442
    Translation

    Target ORF information:

    RefSeq Version XM_004912076.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis zinc finger protein 654 (znf654), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004912076.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCGGAAG ACGAGAGCGA TCAAGAATCG GAGAGACTGG TAGAAGAGCT GGTAGCGATC 
    GCGGATGAAG ACGTGGGGAA TGGCGACTTG TGCCGTGTGA ACAGCAGGGA ATACTGCCGG
    CGCTTTTGCG AGGTTGTTGA AGATTATACT TCAAGATGCC AGGTTCCTTT ACCCCAACTC
    CAAGTCCTCC AAACAGCTCT GTGTTGCTTC ACATCTGCCA CCATATCCTT CCCAGCCGAA
    TGTGAGCACG TACAGTATGT TTTGAGTAGA CTAGCTTTGA GTCTGTTTGA ATTGTTACTC
    TTCTTTGGAA AAGATGAGTT TTACGAGGCT CCTCTGAAAG ACATTCTTGG CTCGGTGCAG
    GAGTGCCACG ACCACCTGAT CCGATATGAT AATACAGATT TAAAGCTGGT CACTTACGTC
    ATTAAGGATG GCGGGCCTTG GGAGAATCCA GTATTACAAG CTATACTCAA GGGAAGATCG
    GAACCACAGG ATATAGTCGA CAGGTATTTG AGGTCTGAGA GTGAGTTCTT CTTTGAGTTG
    CGAGTGAGGT ACCTCATTGC CTGTGAGCGT ATCCCAGAAG CAGTGGCTCT CATCACAACT
    TGCCTAAGTA ACAGCGATGT CAGTAAAAAC CTGTACTACC ATCAAGCGTA CTTCATATGC
    CTACATATGA CCCGATTAAC TGACCAGCTT CTGCACGAGC ATGTTCTAAG GATAAATGCA
    AGAGATGGGG TAGAAATTAT CTGCAGCACT GAGCTGGAAG GTAAAACGGT ACTGGCGTTG
    CAGCTGAGCG AAGCTTTCCT CCTCACACAG CTACAGACTG GGGAGATGGA CTGCATTTGG
    GATTTAATAT TTATTTGGAG CAAGCTTCAG CTGAAAATGA ACTCCTCGAA GCAAGCCTTT
    GTTGAGCAGT GCTATCAGAT GTTAAGGATA GCCACCAACA TTAAAGTCAT CTTCCCTTTC
    ATAAAAACAA TTTTAAATGA AGTTGGAGAA GTCGCTGGGC AGTTGGCTGT AGAGCTTTGT
    GGATGTGCAT TACAATTAGA CCTTCATGAT AACCCAGAAA CCAAATCATT AATTTATAAA
    ACAATTGCTT ACCTTTTGCC CACTGACTTG GAGATTTGCA GGGTCTGCGC TTTATCCGTT
    TTCTTCTTGG AGAGGACCGC CGAATCCTAT GCTGCAGTCG AACGCTTGTA CAAATGTTCC
    GACGAGGATT ACAATGAATT TTTGAGCTGT GTTGAAAACC GGGTGCGTTT TGAATTGCTT
    CCCATTCTGA AAAGGGGGTT AATGTTTGAT CCAGAGTTTT GGAATTTCTC TATGATCGAG
    GAAAAATGTA CTTCTCTCCT TGGGGAAGAT GAGCCTCCAG CATCATGTAA TGCAAGTACA
    CTGGATACTG AAAATTCAGA TACCTCTAAT ATGGACTTGA AAAATGGGGT CATTGACAAT
    GGAGAGTCCA GGAGCACCAG ATCAAAAATC TCTCTTGTTA AAAAGAGTAT TCCTCCTATG
    CTTGGCTCTT ACAAGATAGA TCATAATGTT CCCGTTCATC AGTGTACCCT GTGCAACAAG
    GTGATCCTTG GTGGCCATAT TTTCAGACAT GCTCAGGCTC ATCAAAAAAA AGGCTGCTTT
    TCGTGTGTTT TATGCTCCAG GAAGTTCAGA AATCGCGTTA AAATCCTTAA ACATTTAAAG
    AACCACATAA AGAAAATGCA GGTACAGCCA ATGGCTGCAC TTGACGCTGC TTCTGTTGGA
    TTAACAGACT CTGCCTCAAG TGGAGATAAA GAAACATCGT GCTTGGAAAA TGGAAATTCT
    AGTAGTTCCA CCACTGATGT TTTAACTGTT GCCCCAGCCG TAAATATGAC TTATCCAGAA
    TCCATTCATC CCATAATTCC TGTTGAAAAT CACTCAGCTG ATCATATTAA TCAGCCTGAC
    GTAAATGATT CAGAGGACGC AGTTGATGTC ACGTCCAGTG CTAACGATGC CTCTGACTGC
    ATTAAAATAA ATGGGTCTGT TTGCCCTCTT GCAGCAAAGC AGGCTGTAAA GGAGCAAGGT
    GTTTATAAAT GCCCGGCTCA AGGGTGTATA CGTCTTTTTA CAAAGATGAG GTCTCTTAAC
    AAGCATGCCA GGATAGCCCA CCCAGCGGAT GCGAGAGTGC AGCAACACTT AATGAAACAC
    AACAAGGGAA AATGCAGATT CTGTCAGAGA AGGTTTGCCG ATTCCAAACA CTTTTTTGAT
    CACATGAAGA GGCACATTTA TCCCGAGGTC TATTTCTGCC AGCATTTAGA TTGCAGTAAA
    AGGTTCAAGC TAGTGACCCA TCTGGCAGAG CACCTGCTAA GCCATGAATC ATTCCAAGCT
    CAGTGTAGCT TTGTTAACTG TTTTGAGATT TTTGATAAAC TCTCCTCACT TTATGAGCAT
    GAAGCTGTGC ACTATAGTCA GAATCCCTGT GAAGCTGCCA CTCCATATCC TGATGTGGAG
    ATCATGGCCG GTGTTGCTGC TTCCAGTGCT TCTGTAGGCA ATGCTCATTT AGATCAGGTT
    CCTTCAGATG GTTGTACAAA GGACCATATT CTTGCAGAGA ATTCTTCCTT GGGGGAATTG
    CACGACTTGA TGGACAATTA CAAGCACTTC TCTAGGCTTT CTAAAGGAAA GAAACCTGAG
    GACAACACGG AGGTAGCAGA CTTACCAGTA CCTGTTTGGA AAGCAAGAAA GGACACAGTT
    GAACCAAAGA CCTATACACA GACAGAGAAA AAAGTAAATG GGGACATCCA GAACGTTAAT
    AGAAATTCAC AAGAAAGTTC AAATCTGCAA GAGATTGAGC AGCCACAGCC TCCTGAGCAA
    GGAAACTTGG CACAAAGTGA CATTGCCGAT GGAAGTAGTC TGAATAAATG TAACGTGCAG
    GAGCAGACTG TAGTGCAAAC ACCTTGCTTG TTGGATACCG CACCACCTAA TACGGAGACT
    GTTGCAAAAG AGGGTTTGGT TATGGATGAC ATGCAGGACG TCTTTGAAGA AAAAGGTGCT
    GCTGGGTTTG CTTGCTCCAA AAGGCCTCCA GGCTCTCCTC TTACAACATA TGGTAGGGCT
    CCTAAGGCGC CATTTATCAG ACCTCAGCCT CCAAGTTACC TAGATGAACA GTATATCAGT
    ATGCCTAAGC GGAGAAAAAA TGCTGAGGAA CTGACGCATG AAAACGACAA AGTTTCTAAA
    AAATCATCAG AAAGATTCAG ATGTGGGAAG TGCCTAACTA ATTACTCTAG CTCAGAAGCA
    CTTAATGAGC ACACAGCACA AAACAAATGT CAGCTTTATT TTGGGTTTGA CTCCGATGAT
    GAAAGTGCCT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.