rsph10b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	rsph10b
Entrez Gene ID	779504
Full Name	radial spoke head 10 homolog B (Chlamydomonas)
General protein information	Preferred Names radial spoke head 10 homolog B (Chlamydomonas) Names radial spoke head 10 homolog B
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002931975.4	XP_002932021.2	radial spoke head 10 homolog B isoform X2
XM_012971059.2	XP_012826513.1	radial spoke head 10 homolog B isoform X4
XM_002931975.5	XP_002932021.2	radial spoke head 10 homolog B isoform X2
XM_004917978.4	XP_004918035.1	radial spoke head 10 homolog B isoform X1
XM_004917978.3	XP_004918035.1	radial spoke head 10 homolog B isoform X1
XM_012971058.2	XP_012826512.1	radial spoke head 10 homolog B isoform X3
XM_012971058.3	XP_012826512.1	radial spoke head 10 homolog B isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RSPH10B	XP_851257.1
Bos taurus (cattle)	RSPH10B	NP_001160086.1
Mus musculus (house mouse)	Rsph10b	XP_006504958.1
Danio rerio (zebrafish)	zgc:153062	NP_001070227.1
Xenopus tropicalis (tropical clawed frog)	rsph10b	XP_004918035.1
Homo sapiens (human)	RSPH10B2	NP_001093167.1
Homo sapiens (human)	RSPH10B	NP_775836.3
Pan troglodytes (chimpanzee)	LOC738574	XP_003318355.1
Rattus norvegicus (Norway rat)	Rsph10b	NP_001013889.1
Gallus gallus (chicken)	RSPH10B	XP_004945154.1

Gene Ontology

The following rsph10b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rsph10b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa28953	XM_012971059.2 Latest version!	Xenopus tropicalis radial spoke head 10 homolog B (Chlamydomonas) (rsph10b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa16354	XM_004917978.4 Latest version!	Xenopus tropicalis radial spoke head 10 homolog B (Chlamydomonas) (rsph10b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa16355	XM_002931975.5 Latest version!	Xenopus tropicalis radial spoke head 10 homolog B (Chlamydomonas) (rsph10b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa28952	XM_012971058.3 Latest version!	Xenopus tropicalis radial spoke head 10 homolog B (Chlamydomonas) (rsph10b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OXa16355	XM_002931975.4	Xenopus tropicalis radial spoke head 10 homolog B (Chlamydomonas) (rsph10b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa16354	XM_004917978.3	Xenopus tropicalis radial spoke head 10 homolog B (Chlamydomonas) (rsph10b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa28952	XM_012971058.2	Xenopus tropicalis radial spoke head 10 homolog B (Chlamydomonas) (rsph10b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rsph10b ( XM_004917978.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004918035.1 Xenopus tropicalis radial spoke head 10 homolog B (Chlamydomonas) (rsph10b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa16354
Clone ID Related Accession (Same CDS sequence)	XM_004917978.4 , XM_004917978.3
Accession Version	XM_004917978.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	radial spoke head 10 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917978.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAAA TGAAGAAAAA AGACAGCAAA AAGGGAGAGA GGGAGAGATC TGCACAAGAC 
ACCCCTGAGG AATGCAGCTT TAAGTTATCT CCAGTTTCCA TCACTCCATC TGAAGAACAA 
CATTCCCATT CCTCTTCCAT GAAATCCATT CCCCAGGAAG AACACATGAA GCAGACTGAA 
CCTCCTGTTG TGACTCCTGC AACAGATGTG TATGAAGAAC CTGTGCTAAC TCAACTTATT 
GTTGAAAAAT ATGAAGGAGA ATTGCTAAAT GGATTGTATG AAGGGGAAGG TGTTGCATAT 
TTTAAAGATG GAAATATTTA TAGAGGAATG TTTTCTGAAG GGATGATGCA TGGCAAAGGA 
AAATACACAT GGAAAGATGG ACTGACATAT GAGGGAGACT TTTACATGAA CTTTCCCATG 
GGACACGGTA TATATAAATG GTCTGATGGC AGCCAATATG AAGGAGAAGT GTATAAGGCA 
ATAAGACATG GGCATGGCAT TTTCACATCC AGTAATCAGA AGGTTTCGTA TGTTGGAGAC 
TGGCATAAAG GCACTCGGCA TGGGAAGGGA GTTATTTACT ATAATAAAGA GGGAACCTCA 
TGGTACGAAG GAGACTGGAT ATCTAACAAG AAGGAGGGCT GGGGAGTGCA GTGCTTTATC 
TCTGGCAACA TTTATGAAGG TCAATGGAAA AACAATATAT TTCATGGGAT GGGGAAAATG 
AGATGGCTGA CATCAAACGA AGAGTATGCA GGGCAGTGGG AAAACGGCAT CCAGAACGGC 
TCTGGTACCC ACACTTGGTT TTTACGGAGG ATTCCAGGTA CACAGTACTC GCTGAGGAAT 
GAATATGTTG GAAACTTTGT CAATGGCATA CGCCAAGGGC AGGGGCAGTT TTATTATGCA 
AATGGTGCTA TGTATGATGG AGAGTGGAAG AATAACAAGA AACACGGACT GGGAAAGTTT 
GTGTTTAAGA ATGGGCAGAT TTATGTTGGA GAGTTTGTGG AGGATCAGAT AGCCGAGTAT 
CCGAATTTCA AGTACGATCG AGTGAATACT CCAGATCTAA GTGGCATCCG GACACAGAGT 
TCCATGAATT TGGCAGGAAG TAGTACATCC AGATACAATA CTGGCATTCC AAGTTTAGCA 
GGGTCTTATA TTGAGCTTGA CTTATCAGCA CTATTATACT CATTCCCGGA GGAGGACAGG 
CAAGAAGAAC TGAAGCAGGT GGAATATGCC GTTCTACGGA ACCTCACTGT CCTGCGTAAG 
ATCTACAAAT TGTACAGCCA GCTGGGAAGT GATGGCTCCC CTGACAATAC CCTTCTCATG 
ACAAAGCTGC AGTTCTGGAG GTTTCTGAAG GATTGCAAGT TTCATCACTA CAGTATCACA 
CTTTCGGAAA TGGACCGTAT CCTAACCGTA GAGAATACTG ATGCTGAAGA GGTTCATTCG 
CCCCATTCCA CAATGCTTCT TCGAACCTTT TTGACTAATA TTATCTACTT GGCATACCAC 
ATCTGTCAAA CAGAAAACCA AGAGAAGAAC ATGTCCCTTG CTGACTGCTT TACTAAAGTC 
ATGGTGCAGA ATATACTTCC CCATGCAACT AATATCAAAG GGTTTCTATT TCCTGATGCT 
CAGAAGGCTT CCTACGCCAT TTCTTATATT GAAAAGTCTT GGCATATCTA CAGAATCTAC 
TGCATGCCAA ACACTTTCCA CCCCTATGAA CCGACCATGA AAATGAGGCA CTTTCTGTTG 
CTAATGAATG ATTTAAAAAT CACAGGACTG ACAGCTACAA ATATTGTTGA CATTTTGGCA 
GCAGATGACC CATGTGTGCG TATTGACCAG GAAGTGAACC TGGAATTGGA GCTAACGTTT 
CTCGAGTTCT TTGAGGCGCT CCTTGGCTGT GCTTCCATAT GTGTTACTGA AGAGCAGATA 
AAGCAGTCAG AGAGCGAATT GTTGAAGAGA GAAGAGTCTC AGGGGAGCTC GATTGGGGAA 
GTCACAGCTT ATCTAAAAGA AGGCCAACCA GAGCATGCTT TTCAGCCCCA AGCTGACATA 
CCGCAGGACC TCTTTCTGAG AAGCTCCATG AACCTTAAGG ATAAGAGACA GAGCCCACCT 
ATTCCAGTCT CTTCTATAAA GAAGCAGCAG AGAGAGGCCA GGGCAGAGAA GTGGTTCCAT 
CAGACTGACA TATTTTTCCT GAAGAAGTTC TTTCCAGCAC ATGAACACAA AGAACGACTG 
AAGGAAGAAA TTCCCAAAAT CAGGGAGAAG CAGGCTGAAG AAGCCAGACG ACAGCAGATT 
AGAGAAGAGG AAGAAGCAAG GCTTAAAGCA GAAAGGGAGG CAGAAGAGGC CAAAAGGGTT 
GAACAGGAGT TGTTGGAAAA GGCCATGGCT GCAATGGAAG CAGAACAGGC AGCAAAGGCA 
CAAGAAGAAG AACAGAAAAG TCCTCAGCCT CCAGTCACTC CTCATGATGA CGCTATCATT 
ATCCCACCAC CATCTGGCAC AAAGACTGCA ACTGCAAGCA AAAAGAAGAG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004918035.1
CDS	119..2635
Translation	MGKMKKKDSKKGERERSAQDTPEECSFKLSPVSITPSEEQHSHSSSMKSIPQEEHMKQTEPPVVTPATDVYEEPVLTQLIVEKYEGELLNGLYEGEGVAYFKDGNIYRGMFSEGMMHGKGKYTWKDGLTYEGDFYMNFPMGHGIYKWSDGSQYEGEVYKAIRHGHGIFTSSNQKVSYVGDWHKGTRHGKGVIYYNKEGTSWYEGDWISNKKEGWGVQCFISGNIYEGQWKNNIFHGMGKMRWLTSNEEYAGQWENGIQNGSGTHTWFLRRIPGTQYSLRNEYVGNFVNGIRQGQGQFYYANGAMYDGEWKNNKKHGLGKFVFKNGQIYVGEFVEDQIAEYPNFKYDRVNTPDLSGIRTQSSMNLAGSSTSRYNTGIPSLAGSYIELDLSALLYSFPEEDRQEELKQVEYAVLRNLTVLRKIYKLYSQLGSDGSPDNTLLMTKLQFWRFLKDCKFHHYSITLSEMDRILTVENTDAEEVHSPHSTMLLRTFLTNIIYLAYHICQTENQEKNMSLADCFTKVMVQNILPHATNIKGFLFPDAQKASYAISYIEKSWHIYRIYCMPNTFHPYEPTMKMRHFLLLMNDLKITGLTATNIVDILAADDPCVRIDQEVNLELELTFLEFFEALLGCASICVTEEQIKQSESELLKREESQGSSIGEVTAYLKEGQPEHAFQPQADIPQDLFLRSSMNLKDKRQSPPIPVSSIKKQQREARAEKWFHQTDIFFLKKFFPAHEHKERLKEEIPKIREKQAEEARRQQIREEEEARLKAEREAEEAKRVEQELLEKAMAAMEAEQAAKAQEEEQKSPQPPVTPHDDAIIIPPPSGTKTATASKKKRK

Target ORF information:

RefSeq Version	XM_004917978.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis radial spoke head 10 homolog B (Chlamydomonas) (rsph10b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917978.3

ORF Insert Sequence:

ATGGGAAAAA TGAAGAAAAA AGACAGCAAA AAGGGAGAGA GGGAGAGATC TGCACAAGAC 
ACCCCTGAGG AATGCAGCTT TAAGTTATCT CCAGTTTCCA TCACTCCATC TGAAGAACAA 
CATTCCCATT CCTCTTCCAT GAAATCCATT CCCCAGGAAG AACACATGAA GCAGACTGAA 
CCTCCTGTTG TGACTCCTGC AACAGATGTG TATGAAGAAC CTGTGCTAAC TCAACTTATT 
GTTGAAAAAT ATGAAGGAGA ATTGCTAAAT GGATTGTATG AAGGGGAAGG TGTTGCATAT 
TTTAAAGATG GAAATATTTA TAGAGGAATG TTTTCTGAAG GGATGATGCA TGGCAAAGGA 
AAATACACAT GGAAAGATGG ACTGACATAT GAGGGAGACT TTTACATGAA CTTTCCCATG 
GGACACGGTA TATATAAATG GTCTGATGGC AGCCAATATG AAGGAGAAGT GTATAAGGCA 
ATAAGACATG GGCATGGCAT TTTCACATCC AGTAATCAGA AGGTTTCGTA TGTTGGAGAC 
TGGCATAAAG GCACTCGGCA TGGGAAGGGA GTTATTTACT ATAATAAAGA GGGAACCTCA 
TGGTACGAAG GAGACTGGAT ATCTAACAAG AAGGAGGGCT GGGGAGTGCA GTGCTTTATC 
TCTGGCAACA TTTATGAAGG TCAATGGAAA AACAATATAT TTCATGGGAT GGGGAAAATG 
AGATGGCTGA CATCAAACGA AGAGTATGCA GGGCAGTGGG AAAACGGCAT CCAGAACGGC 
TCTGGTACCC ACACTTGGTT TTTACGGAGG ATTCCAGGTA CACAGTACTC GCTGAGGAAT 
GAATATGTTG GAAACTTTGT CAATGGCATA CGCCAAGGGC AGGGGCAGTT TTATTATGCA 
AATGGTGCTA TGTATGATGG AGAGTGGAAG AATAACAAGA AACACGGACT GGGAAAGTTT 
GTGTTTAAGA ATGGGCAGAT TTATGTTGGA GAGTTTGTGG AGGATCAGAT AGCCGAGTAT 
CCGAATTTCA AGTACGATCG AGTGAATACT CCAGATCTAA GTGGCATCCG GACACAGAGT 
TCCATGAATT TGGCAGGAAG TAGTACATCC AGATACAATA CTGGCATTCC AAGTTTAGCA 
GGGTCTTATA TTGAGCTTGA CTTATCAGCA CTATTATACT CATTCCCGGA GGAGGACAGG 
CAAGAAGAAC TGAAGCAGGT GGAATATGCC GTTCTACGGA ACCTCACTGT CCTGCGTAAG 
ATCTACAAAT TGTACAGCCA GCTGGGAAGT GATGGCTCCC CTGACAATAC CCTTCTCATG 
ACAAAGCTGC AGTTCTGGAG GTTTCTGAAG GATTGCAAGT TTCATCACTA CAGTATCACA 
CTTTCGGAAA TGGACCGTAT CCTAACCGTA GAGAATACTG ATGCTGAAGA GGTTCATTCG 
CCCCATTCCA CAATGCTTCT TCGAACCTTT TTGACTAATA TTATCTACTT GGCATACCAC 
ATCTGTCAAA CAGAAAACCA AGAGAAGAAC ATGTCCCTTG CTGACTGCTT TACTAAAGTC 
ATGGTGCAGA ATATACTTCC CCATGCAACT AATATCAAAG GGTTTCTATT TCCTGATGCT 
CAGAAGGCTT CCTACGCCAT TTCTTATATT GAAAAGTCTT GGCATATCTA CAGAATCTAC 
TGCATGCCAA ACACTTTCCA CCCCTATGAA CCGACCATGA AAATGAGGCA CTTTCTGTTG 
CTAATGAATG ATTTAAAAAT CACAGGACTG ACAGCTACAA ATATTGTTGA CATTTTGGCA 
GCAGATGACC CATGTGTGCG TATTGACCAG GAAGTGAACC TGGAATTGGA GCTAACGTTT 
CTCGAGTTCT TTGAGGCGCT CCTTGGCTGT GCTTCCATAT GTGTTACTGA AGAGCAGATA 
AAGCAGTCAG AGAGCGAATT GTTGAAGAGA GAAGAGTCTC AGGGGAGCTC GATTGGGGAA 
GTCACAGCTT ATCTAAAAGA AGGCCAACCA GAGCATGCTT TTCAGCCCCA AGCTGACATA 
CCGCAGGACC TCTTTCTGAG AAGCTCCATG AACCTTAAGG ATAAGAGACA GAGCCCACCT 
ATTCCAGTCT CTTCTATAAA GAAGCAGCAG AGAGAGGCCA GGGCAGAGAA GTGGTTCCAT 
CAGACTGACA TATTTTTCCT GAAGAAGTTC TTTCCAGCAC ATGAACACAA AGAACGACTG 
AAGGAAGAAA TTCCCAAAAT CAGGGAGAAG CAGGCTGAAG AAGCCAGACG ACAGCAGATT 
AGAGAAGAGG AAGAAGCAAG GCTTAAAGCA GAAAGGGAGG CAGAAGAGGC CAAAAGGGTT 
GAACAGGAGT TGTTGGAAAA GGCCATGGCT GCAATGGAAG CAGAACAGGC AGCAAAGGCA 
CAAGAAGAAG AACAGAAAAG TCCTCAGCCT CCAGTCACTC CTCATGATGA CGCTATCATT 
ATCCCACCAC CATCTGGCAC AAAGACTGCA ACTGCAAGCA AAAAGAAGAG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rsph10b ( XM_002931975.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932021.2 Xenopus tropicalis radial spoke head 10 homolog B (Chlamydomonas) (rsph10b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa16355
Clone ID Related Accession (Same CDS sequence)	XM_002931975.4 , XM_002931975.5
Accession Version	XM_002931975.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	radial spoke head 10 homolog B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002931975.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAAA TGAAGAAAAA AGACAGCAAA AAGGGAGAGA GGGAGAGATC TGCACAAGAC 
ACCCCTGAGG AATGCAGCTT TAAGTTATCT CCAGTTTCCA TCACTCCATC TGAAGAACAA 
CATTCCCATT CCTCTTCCAT GAAATCCATT CCCCAGGAAG AACACATGAA GCAGACTGAA 
CCTCCTGTTG TGACTCCTGC AACAGATGTG TATGAAGAAC CTGTGCTAAC TCAACTTATT 
GTTGAAAAAT ATGAAGGAGA ATTGCTAAAT GGATTGTATG AAGGGGAAGG TGTTGCATAT 
TTTAAAGATG GAAATATTTA TAGAGGAATG TTTTCTGAAG GGATGATGCA TGGCAAAGGA 
AAATACACAT GGAAAGATGG ACTGACATAT GAGGGAGACT TTTACATGAA CTTTCCCATG 
GGACACGGTA TATATAAATG GTCTGATGGC AGCCAATATG AAGGAGAAGT GTATAAGGCA 
ATAAGACATG GGCATGGCAT TTTCACATCC AGTAATCAGA AGGTTTCGTA TGTTGGAGAC 
TGGCATAAAG GCACTCGGCA TGGGAAGGGA GTTATTTACT ATAATAAAGA GGGAACCTCA 
TGGTACGAAG GAGACTGGAT ATCTAACAAG AAGGAGGGCT GGGGAGTGCA GTGCTTTATC 
TCTGGCAACA TTTATGAAGG TCAATGGAAA AACAATATAT TTCATGGGAT GGGGAAAATG 
AGATGGCTGA CATCAAACGA AGAGTATGCA GGGCAGTGGG AAAACGGCAT CCAGAACGGC 
TCTGGTACCC ACACTTGGTT TTTACGGAGG ATTCCAGGTA CACAGTACTC GCTGAGGAAT 
GAATATGTTG GAAACTTTGT CAATGGCATA CGCCAAGGGC AGGGGCAGTT TTATTATGCA 
AATGGTGCTA TGTATGATGG AGAGTGGAAG AATAACAAGA AACACGGACT GGGAAAGTTT 
GTGTTTAAGA ATGGGCAGAT TTATGTTGGA GAGTTTGTGG AGGATCAGAT AGCCGAGTAT 
CCGAATTTCA AGTACGATCG AGTGAATACT CCAGATCTAA GTGGCATCCG GACACAGAGT 
TCCATGAATT TGGGAAGTAG TACATCCAGA TACAATACTG GCATTCCAAG TTTAGCAGGG 
TCTTATATTG AGCTTGACTT ATCAGCACTA TTATACTCAT TCCCGGAGGA GGACAGGCAA 
GAAGAACTGA AGCAGGTGGA ATATGCCGTT CTACGGAACC TCACTGTCCT GCGTAAGATC 
TACAAATTGT ACAGCCAGCT GGGAAGTGAT GGCTCCCCTG ACAATACCCT TCTCATGACA 
AAGCTGCAGT TCTGGAGGTT TCTGAAGGAT TGCAAGTTTC ATCACTACAG TATCACACTT 
TCGGAAATGG ACCGTATCCT AACCGTAGAG AATACTGATG CTGAAGAGGT TCATTCGCCC 
CATTCCACAA TGCTTCTTCG AACCTTTTTG ACTAATATTA TCTACTTGGC ATACCACATC 
TGTCAAACAG AAAACCAAGA GAAGAACATG TCCCTTGCTG ACTGCTTTAC TAAAGTCATG 
GTGCAGAATA TACTTCCCCA TGCAACTAAT ATCAAAGGGT TTCTATTTCC TGATGCTCAG 
AAGGCTTCCT ACGCCATTTC TTATATTGAA AAGTCTTGGC ATATCTACAG AATCTACTGC 
ATGCCAAACA CTTTCCACCC CTATGAACCG ACCATGAAAA TGAGGCACTT TCTGTTGCTA 
ATGAATGATT TAAAAATCAC AGGACTGACA GCTACAAATA TTGTTGACAT TTTGGCAGCA 
GATGACCCAT GTGTGCGTAT TGACCAGGAA GTGAACCTGG AATTGGAGCT AACGTTTCTC 
GAGTTCTTTG AGGCGCTCCT TGGCTGTGCT TCCATATGTG TTACTGAAGA GCAGATAAAG 
CAGTCAGAGA GCGAATTGTT GAAGAGAGAA GAGTCTCAGG GGAGCTCGAT TGGGGAAGTC 
ACAGCTTATC TAAAAGAAGG CCAACCAGAG CATGCTTTTC AGCCCCAAGC TGACATACCG 
CAGGACCTCT TTCTGAGAAG CTCCATGAAC CTTAAGGATA AGAGACAGAG CCCACCTATT 
CCAGTCTCTT CTATAAAGAA GCAGCAGAGA GAGGCCAGGG CAGAGAAGTG GTTCCATCAG 
ACTGACATAT TTTTCCTGAA GAAGTTCTTT CCAGCACATG AACACAAAGA ACGACTGAAG 
GAAGAAATTC CCAAAATCAG GGAGAAGCAG GCTGAAGAAG CCAGACGACA GCAGATTAGA 
GAAGAGGAAG AAGCAAGGCT TAAAGCAGAA AGGGAGGCAG AAGAGGCCAA AAGGGTTGAA 
CAGGAGTTGT TGGAAAAGGC CATGGCTGCA ATGGAAGCAG AACAGGCAGC AAAGGCACAA 
GAAGAAGAAC AGAAAAGTCC TCAGCCTCCA GTCACTCCTC ATGATGACGC TATCATTATC 
CCACCACCAT CTGGCACAAA GACTGCAACT GCAAGCAAAA AGAAGAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932021.2
CDS	119..2632
Translation	MGKMKKKDSKKGERERSAQDTPEECSFKLSPVSITPSEEQHSHSSSMKSIPQEEHMKQTEPPVVTPATDVYEEPVLTQLIVEKYEGELLNGLYEGEGVAYFKDGNIYRGMFSEGMMHGKGKYTWKDGLTYEGDFYMNFPMGHGIYKWSDGSQYEGEVYKAIRHGHGIFTSSNQKVSYVGDWHKGTRHGKGVIYYNKEGTSWYEGDWISNKKEGWGVQCFISGNIYEGQWKNNIFHGMGKMRWLTSNEEYAGQWENGIQNGSGTHTWFLRRIPGTQYSLRNEYVGNFVNGIRQGQGQFYYANGAMYDGEWKNNKKHGLGKFVFKNGQIYVGEFVEDQIAEYPNFKYDRVNTPDLSGIRTQSSMNLGSSTSRYNTGIPSLAGSYIELDLSALLYSFPEEDRQEELKQVEYAVLRNLTVLRKIYKLYSQLGSDGSPDNTLLMTKLQFWRFLKDCKFHHYSITLSEMDRILTVENTDAEEVHSPHSTMLLRTFLTNIIYLAYHICQTENQEKNMSLADCFTKVMVQNILPHATNIKGFLFPDAQKASYAISYIEKSWHIYRIYCMPNTFHPYEPTMKMRHFLLLMNDLKITGLTATNIVDILAADDPCVRIDQEVNLELELTFLEFFEALLGCASICVTEEQIKQSESELLKREESQGSSIGEVTAYLKEGQPEHAFQPQADIPQDLFLRSSMNLKDKRQSPPIPVSSIKKQQREARAEKWFHQTDIFFLKKFFPAHEHKERLKEEIPKIREKQAEEARRQQIREEEEARLKAEREAEEAKRVEQELLEKAMAAMEAEQAAKAQEEEQKSPQPPVTPHDDAIIIPPPSGTKTATASKKKRK

Target ORF information:

RefSeq Version	XM_002931975.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis radial spoke head 10 homolog B (Chlamydomonas) (rsph10b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931975.4

ORF Insert Sequence:

ATGGGAAAAA TGAAGAAAAA AGACAGCAAA AAGGGAGAGA GGGAGAGATC TGCACAAGAC 
ACCCCTGAGG AATGCAGCTT TAAGTTATCT CCAGTTTCCA TCACTCCATC TGAAGAACAA 
CATTCCCATT CCTCTTCCAT GAAATCCATT CCCCAGGAAG AACACATGAA GCAGACTGAA 
CCTCCTGTTG TGACTCCTGC AACAGATGTG TATGAAGAAC CTGTGCTAAC TCAACTTATT 
GTTGAAAAAT ATGAAGGAGA ATTGCTAAAT GGATTGTATG AAGGGGAAGG TGTTGCATAT 
TTTAAAGATG GAAATATTTA TAGAGGAATG TTTTCTGAAG GGATGATGCA TGGCAAAGGA 
AAATACACAT GGAAAGATGG ACTGACATAT GAGGGAGACT TTTACATGAA CTTTCCCATG 
GGACACGGTA TATATAAATG GTCTGATGGC AGCCAATATG AAGGAGAAGT GTATAAGGCA 
ATAAGACATG GGCATGGCAT TTTCACATCC AGTAATCAGA AGGTTTCGTA TGTTGGAGAC 
TGGCATAAAG GCACTCGGCA TGGGAAGGGA GTTATTTACT ATAATAAAGA GGGAACCTCA 
TGGTACGAAG GAGACTGGAT ATCTAACAAG AAGGAGGGCT GGGGAGTGCA GTGCTTTATC 
TCTGGCAACA TTTATGAAGG TCAATGGAAA AACAATATAT TTCATGGGAT GGGGAAAATG 
AGATGGCTGA CATCAAACGA AGAGTATGCA GGGCAGTGGG AAAACGGCAT CCAGAACGGC 
TCTGGTACCC ACACTTGGTT TTTACGGAGG ATTCCAGGTA CACAGTACTC GCTGAGGAAT 
GAATATGTTG GAAACTTTGT CAATGGCATA CGCCAAGGGC AGGGGCAGTT TTATTATGCA 
AATGGTGCTA TGTATGATGG AGAGTGGAAG AATAACAAGA AACACGGACT GGGAAAGTTT 
GTGTTTAAGA ATGGGCAGAT TTATGTTGGA GAGTTTGTGG AGGATCAGAT AGCCGAGTAT 
CCGAATTTCA AGTACGATCG AGTGAATACT CCAGATCTAA GTGGCATCCG GACACAGAGT 
TCCATGAATT TGGGAAGTAG TACATCCAGA TACAATACTG GCATTCCAAG TTTAGCAGGG 
TCTTATATTG AGCTTGACTT ATCAGCACTA TTATACTCAT TCCCGGAGGA GGACAGGCAA 
GAAGAACTGA AGCAGGTGGA ATATGCCGTT CTACGGAACC TCACTGTCCT GCGTAAGATC 
TACAAATTGT ACAGCCAGCT GGGAAGTGAT GGCTCCCCTG ACAATACCCT TCTCATGACA 
AAGCTGCAGT TCTGGAGGTT TCTGAAGGAT TGCAAGTTTC ATCACTACAG TATCACACTT 
TCGGAAATGG ACCGTATCCT AACCGTAGAG AATACTGATG CTGAAGAGGT TCATTCGCCC 
CATTCCACAA TGCTTCTTCG AACCTTTTTG ACTAATATTA TCTACTTGGC ATACCACATC 
TGTCAAACAG AAAACCAAGA GAAGAACATG TCCCTTGCTG ACTGCTTTAC TAAAGTCATG 
GTGCAGAATA TACTTCCCCA TGCAACTAAT ATCAAAGGGT TTCTATTTCC TGATGCTCAG 
AAGGCTTCCT ACGCCATTTC TTATATTGAA AAGTCTTGGC ATATCTACAG AATCTACTGC 
ATGCCAAACA CTTTCCACCC CTATGAACCG ACCATGAAAA TGAGGCACTT TCTGTTGCTA 
ATGAATGATT TAAAAATCAC AGGACTGACA GCTACAAATA TTGTTGACAT TTTGGCAGCA 
GATGACCCAT GTGTGCGTAT TGACCAGGAA GTGAACCTGG AATTGGAGCT AACGTTTCTC 
GAGTTCTTTG AGGCGCTCCT TGGCTGTGCT TCCATATGTG TTACTGAAGA GCAGATAAAG 
CAGTCAGAGA GCGAATTGTT GAAGAGAGAA GAGTCTCAGG GGAGCTCGAT TGGGGAAGTC 
ACAGCTTATC TAAAAGAAGG CCAACCAGAG CATGCTTTTC AGCCCCAAGC TGACATACCG 
CAGGACCTCT TTCTGAGAAG CTCCATGAAC CTTAAGGATA AGAGACAGAG CCCACCTATT 
CCAGTCTCTT CTATAAAGAA GCAGCAGAGA GAGGCCAGGG CAGAGAAGTG GTTCCATCAG 
ACTGACATAT TTTTCCTGAA GAAGTTCTTT CCAGCACATG AACACAAAGA ACGACTGAAG 
GAAGAAATTC CCAAAATCAG GGAGAAGCAG GCTGAAGAAG CCAGACGACA GCAGATTAGA 
GAAGAGGAAG AAGCAAGGCT TAAAGCAGAA AGGGAGGCAG AAGAGGCCAA AAGGGTTGAA 
CAGGAGTTGT TGGAAAAGGC CATGGCTGCA ATGGAAGCAG AACAGGCAGC AAAGGCACAA 
GAAGAAGAAC AGAAAAGTCC TCAGCCTCCA GTCACTCCTC ATGATGACGC TATCATTATC 
CCACCACCAT CTGGCACAAA GACTGCAACT GCAAGCAAAA AGAAGAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rsph10b ( XM_012971058.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826512.1 Xenopus tropicalis radial spoke head 10 homolog B (Chlamydomonas) (rsph10b), transcript variant X3, mRNA.

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CloneID	OXa28952
Clone ID Related Accession (Same CDS sequence)	XM_012971058.2 , XM_012971058.3
Accession Version	XM_012971058.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	radial spoke head 10 homolog B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971058.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAAA TGAAGAAAAA AGACAGCAAA AAGGGAGAGA GGGAGAGATC TGCACAAGAC 
ACCCCTGAGG AATGCAGCTT TAAGTTATCT CCAGTTTCCA TCACTCCATC TGAAGAACAA 
CATTCCCATT CCTCTTCCAT GAAATCCATT CCCCAGGAAG AACACATGAA GCAGACTGAA 
CCTCCTGTTG TGACTCCTGC AACAGATGTG TATGAAGAAC CTGTGCTAAC TCAACTTATT 
GTTGAAAAAT ATGAAGGAGA ATTGCTAAAT GGATTGTATG AAGGGGAAGG TGTTGCATAT 
TTTAAAGATG GAAATATTTA TAGAGGAATG TTTTCTGAAG GGATGATGCA TGGCAAAGGA 
AAATACACAT GGAAAGATGG ACTGACATAT GAGGGAGACT TTTACATGAA CTTTCCCATG 
GGACACGGTA TATATAAATG GTCTGATGGC AGCCAATATG AAGGAGAAGT GTATAAGGCA 
ATAAGACATG GGCATGGCAT TTTCACATCC AGTAATCAGA AGGTTTCGTA TGTTGGAGAC 
TGGCATAAAG GCACTCGGCA TGGGAAGGGA GTTATTTACT ATAATAAAGA GGGAACCTCA 
TGGTACGAAG GAGACTGGAT ATCTAACAAG AAGGAGGGCT GGGGAGTGCA GTGCTTTATC 
TCTGGCAACA TTTATGAAGG TCAATGGAAA AACAATATAT TTCATGGGAT GGGGAAAATG 
AGATGGCTGA CATCAAACGA AGAGTATGCA GGGCAGTGGG AAAACGGCAT CCAGAACGGC 
TCTGGTACCC ACACTTGGTT TTTACGGAGG ATTCCAGGTA CACAGTACTC GCTGAGGAAT 
GAATATGTTG GAAACTTTGT CAATGGCATA CGCCAAGGGC AGGGGCAGTT TTATTATGCA 
AATGGTGCTA TGTATGATGG AGAGTGGAAG AATAACAAGA AACACGGACT GGGAAAGTTT 
GTGTTTAAGA ATGGGCAGAT TTATGTTGGA GAGTTTGTGG AGGATCAGAT AGCCGAGTAT 
CCGAATTTCA AGTACGATCG AGTGAATACT CCAGATCTAA GTGGCATCCG GACACAGAGT 
TCCATGAATT TGGCAGGAAG TAGTACATCC AGATACAATA CTGGCATTCC AAGTTTAGCA 
GGGTCTTATA TTGAGCTTGA CTTATCAGCA CTATTATACT CATTCCCGGA GGAGGACAGG 
CAAGAAGAAC TGAAGCAGGT GGAATATGCC GTTCTACGGA ACCTCACTGT CCTGCGTAAG 
ATCTACAAAT TGTACAGCCA GCTGGGAAGT GATGGCTCCC CTGACAATAC CCTTCTCATG 
ACAAAGCTGC AGTTCTGGAG GTTTCTGAAG GATTGCAAGT TTCATCACTA CAGTATCACA 
CTTTCGGAAA TGGACCGTAT CCTAACCGAG AATACTGATG CTGAAGAGGT TCATTCGCCC 
CATTCCACAA TGCTTCTTCG AACCTTTTTG ACTAATATTA TCTACTTGGC ATACCACATC 
TGTCAAACAG AAAACCAAGA GAAGAACATG TCCCTTGCTG ACTGCTTTAC TAAAGTCATG 
GTGCAGAATA TACTTCCCCA TGCAACTAAT ATCAAAGGGT TTCTATTTCC TGATGCTCAG 
AAGGCTTCCT ACGCCATTTC TTATATTGAA AAGTCTTGGC ATATCTACAG AATCTACTGC 
ATGCCAAACA CTTTCCACCC CTATGAACCG ACCATGAAAA TGAGGCACTT TCTGTTGCTA 
ATGAATGATT TAAAAATCAC AGGACTGACA GCTACAAATA TTGTTGACAT TTTGGCAGCA 
GATGACCCAT GTGTGCGTAT TGACCAGGAA GTGAACCTGG AATTGGAGCT AACGTTTCTC 
GAGTTCTTTG AGGCGCTCCT TGGCTGTGCT TCCATATGTG TTACTGAAGA GCAGATAAAG 
CAGTCAGAGA GCGAATTGTT GAAGAGAGAA GAGTCTCAGG GGAGCTCGAT TGGGGAAGTC 
ACAGCTTATC TAAAAGAAGG CCAACCAGAG CATGCTTTTC AGCCCCAAGC TGACATACCG 
CAGGACCTCT TTCTGAGAAG CTCCATGAAC CTTAAGGATA AGAGACAGAG CCCACCTATT 
CCAGTCTCTT CTATAAAGAA GCAGCAGAGA GAGGCCAGGG CAGAGAAGTG GTTCCATCAG 
ACTGACATAT TTTTCCTGAA GAAGTTCTTT CCAGCACATG AACACAAAGA ACGACTGAAG 
GAAGAAATTC CCAAAATCAG GGAGAAGCAG GCTGAAGAAG CCAGACGACA GCAGATTAGA 
GAAGAGGAAG AAGCAAGGCT TAAAGCAGAA AGGGAGGCAG AAGAGGCCAA AAGGGTTGAA 
CAGGAGTTGT TGGAAAAGGC CATGGCTGCA ATGGAAGCAG AACAGGCAGC AAAGGCACAA 
GAAGAAGAAC AGAAAAGTCC TCAGCCTCCA GTCACTCCTC ATGATGACGC TATCATTATC 
CCACCACCAT CTGGCACAAA GACTGCAACT GCAAGCAAAA AGAAGAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826512.1
CDS	119..2632
Translation	MGKMKKKDSKKGERERSAQDTPEECSFKLSPVSITPSEEQHSHSSSMKSIPQEEHMKQTEPPVVTPATDVYEEPVLTQLIVEKYEGELLNGLYEGEGVAYFKDGNIYRGMFSEGMMHGKGKYTWKDGLTYEGDFYMNFPMGHGIYKWSDGSQYEGEVYKAIRHGHGIFTSSNQKVSYVGDWHKGTRHGKGVIYYNKEGTSWYEGDWISNKKEGWGVQCFISGNIYEGQWKNNIFHGMGKMRWLTSNEEYAGQWENGIQNGSGTHTWFLRRIPGTQYSLRNEYVGNFVNGIRQGQGQFYYANGAMYDGEWKNNKKHGLGKFVFKNGQIYVGEFVEDQIAEYPNFKYDRVNTPDLSGIRTQSSMNLAGSSTSRYNTGIPSLAGSYIELDLSALLYSFPEEDRQEELKQVEYAVLRNLTVLRKIYKLYSQLGSDGSPDNTLLMTKLQFWRFLKDCKFHHYSITLSEMDRILTENTDAEEVHSPHSTMLLRTFLTNIIYLAYHICQTENQEKNMSLADCFTKVMVQNILPHATNIKGFLFPDAQKASYAISYIEKSWHIYRIYCMPNTFHPYEPTMKMRHFLLLMNDLKITGLTATNIVDILAADDPCVRIDQEVNLELELTFLEFFEALLGCASICVTEEQIKQSESELLKREESQGSSIGEVTAYLKEGQPEHAFQPQADIPQDLFLRSSMNLKDKRQSPPIPVSSIKKQQREARAEKWFHQTDIFFLKKFFPAHEHKERLKEEIPKIREKQAEEARRQQIREEEEARLKAEREAEEAKRVEQELLEKAMAAMEAEQAAKAQEEEQKSPQPPVTPHDDAIIIPPPSGTKTATASKKKRK

Target ORF information:

RefSeq Version	XM_012971058.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis radial spoke head 10 homolog B (Chlamydomonas) (rsph10b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971058.2

ORF Insert Sequence:

ATGGGAAAAA TGAAGAAAAA AGACAGCAAA AAGGGAGAGA GGGAGAGATC TGCACAAGAC 
ACCCCTGAGG AATGCAGCTT TAAGTTATCT CCAGTTTCCA TCACTCCATC TGAAGAACAA 
CATTCCCATT CCTCTTCCAT GAAATCCATT CCCCAGGAAG AACACATGAA GCAGACTGAA 
CCTCCTGTTG TGACTCCTGC AACAGATGTG TATGAAGAAC CTGTGCTAAC TCAACTTATT 
GTTGAAAAAT ATGAAGGAGA ATTGCTAAAT GGATTGTATG AAGGGGAAGG TGTTGCATAT 
TTTAAAGATG GAAATATTTA TAGAGGAATG TTTTCTGAAG GGATGATGCA TGGCAAAGGA 
AAATACACAT GGAAAGATGG ACTGACATAT GAGGGAGACT TTTACATGAA CTTTCCCATG 
GGACACGGTA TATATAAATG GTCTGATGGC AGCCAATATG AAGGAGAAGT GTATAAGGCA 
ATAAGACATG GGCATGGCAT TTTCACATCC AGTAATCAGA AGGTTTCGTA TGTTGGAGAC 
TGGCATAAAG GCACTCGGCA TGGGAAGGGA GTTATTTACT ATAATAAAGA GGGAACCTCA 
TGGTACGAAG GAGACTGGAT ATCTAACAAG AAGGAGGGCT GGGGAGTGCA GTGCTTTATC 
TCTGGCAACA TTTATGAAGG TCAATGGAAA AACAATATAT TTCATGGGAT GGGGAAAATG 
AGATGGCTGA CATCAAACGA AGAGTATGCA GGGCAGTGGG AAAACGGCAT CCAGAACGGC 
TCTGGTACCC ACACTTGGTT TTTACGGAGG ATTCCAGGTA CACAGTACTC GCTGAGGAAT 
GAATATGTTG GAAACTTTGT CAATGGCATA CGCCAAGGGC AGGGGCAGTT TTATTATGCA 
AATGGTGCTA TGTATGATGG AGAGTGGAAG AATAACAAGA AACACGGACT GGGAAAGTTT 
GTGTTTAAGA ATGGGCAGAT TTATGTTGGA GAGTTTGTGG AGGATCAGAT AGCCGAGTAT 
CCGAATTTCA AGTACGATCG AGTGAATACT CCAGATCTAA GTGGCATCCG GACACAGAGT 
TCCATGAATT TGGCAGGAAG TAGTACATCC AGATACAATA CTGGCATTCC AAGTTTAGCA 
GGGTCTTATA TTGAGCTTGA CTTATCAGCA CTATTATACT CATTCCCGGA GGAGGACAGG 
CAAGAAGAAC TGAAGCAGGT GGAATATGCC GTTCTACGGA ACCTCACTGT CCTGCGTAAG 
ATCTACAAAT TGTACAGCCA GCTGGGAAGT GATGGCTCCC CTGACAATAC CCTTCTCATG 
ACAAAGCTGC AGTTCTGGAG GTTTCTGAAG GATTGCAAGT TTCATCACTA CAGTATCACA 
CTTTCGGAAA TGGACCGTAT CCTAACCGAG AATACTGATG CTGAAGAGGT TCATTCGCCC 
CATTCCACAA TGCTTCTTCG AACCTTTTTG ACTAATATTA TCTACTTGGC ATACCACATC 
TGTCAAACAG AAAACCAAGA GAAGAACATG TCCCTTGCTG ACTGCTTTAC TAAAGTCATG 
GTGCAGAATA TACTTCCCCA TGCAACTAAT ATCAAAGGGT TTCTATTTCC TGATGCTCAG 
AAGGCTTCCT ACGCCATTTC TTATATTGAA AAGTCTTGGC ATATCTACAG AATCTACTGC 
ATGCCAAACA CTTTCCACCC CTATGAACCG ACCATGAAAA TGAGGCACTT TCTGTTGCTA 
ATGAATGATT TAAAAATCAC AGGACTGACA GCTACAAATA TTGTTGACAT TTTGGCAGCA 
GATGACCCAT GTGTGCGTAT TGACCAGGAA GTGAACCTGG AATTGGAGCT AACGTTTCTC 
GAGTTCTTTG AGGCGCTCCT TGGCTGTGCT TCCATATGTG TTACTGAAGA GCAGATAAAG 
CAGTCAGAGA GCGAATTGTT GAAGAGAGAA GAGTCTCAGG GGAGCTCGAT TGGGGAAGTC 
ACAGCTTATC TAAAAGAAGG CCAACCAGAG CATGCTTTTC AGCCCCAAGC TGACATACCG 
CAGGACCTCT TTCTGAGAAG CTCCATGAAC CTTAAGGATA AGAGACAGAG CCCACCTATT 
CCAGTCTCTT CTATAAAGAA GCAGCAGAGA GAGGCCAGGG CAGAGAAGTG GTTCCATCAG 
ACTGACATAT TTTTCCTGAA GAAGTTCTTT CCAGCACATG AACACAAAGA ACGACTGAAG 
GAAGAAATTC CCAAAATCAG GGAGAAGCAG GCTGAAGAAG CCAGACGACA GCAGATTAGA 
GAAGAGGAAG AAGCAAGGCT TAAAGCAGAA AGGGAGGCAG AAGAGGCCAA AAGGGTTGAA 
CAGGAGTTGT TGGAAAAGGC CATGGCTGCA ATGGAAGCAG AACAGGCAGC AAAGGCACAA 
GAAGAAGAAC AGAAAAGTCC TCAGCCTCCA GTCACTCCTC ATGATGACGC TATCATTATC 
CCACCACCAT CTGGCACAAA GACTGCAACT GCAAGCAAAA AGAAGAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rsph10b ( XM_012971059.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826513.1 Xenopus tropicalis radial spoke head 10 homolog B (Chlamydomonas) (rsph10b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa28953
Clone ID Related Accession (Same CDS sequence)	XM_012971059.2
Accession Version	XM_012971059.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	radial spoke head 10 homolog B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971059.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAAA TGAAGAAAAA AGACAGCAAA AAGGGAGAGA GGGAGAGATC TGCACAAGAC 
ACCCCTGAGG AATGCAGCTT TAAGTTATCT CCAGTTTCCA TCACTCCATC TGAAGAACAA 
CATTCCCATT CCTCTTCCAT GAAATCCATT CCCCAGGAAG AACACATGAA GCAGACTGAA 
CCTCCTGTTG TGACTCCTGC AACAGATGTG TATGAAGAAC CTGTGCTAAC TCAACTTATT 
GTTGAAAAAT ATGAAGGAGA ATTGCTAAAT GGATTGTATG AAGGGGAAGG TGTTGCATAT 
TTTAAAGATG GAAATATTTA TAGAGGAATG TTTTCTGAAG GGATGATGCA TGGCAAAGGA 
AAATACACAT GGAAAGATGG ACTGACATAT GAGGGAGACT TTTACATGAA CTTTCCCATG 
GGACACGGTA TATATAAATG GTCTGATGGC AGCCAATATG AAGGAGAAGT GTATAAGGCA 
ATAAGACATG GGCATGGCAT TTTCACATCC AGTAATCAGA AGGTTTCGTA TGTTGGAGAC 
TGGCATAAAG GCACTCGGCA TGGGAAGGGA GTTATTTACT ATAATAAAGA GGGAACCTCA 
TGGTACGAAG GAGACTGGAT ATCTAACAAG AAGGAGGGCT GGGGAGTGCA GTGCTTTATC 
TCTGGCAACA TTTATGAAGG TCAATGGAAA AACAATATAT TTCATGGGAT GGGGAAAATG 
AGATGGCTGA CATCAAACGA AGAGTATGCA GGGCAGTGGG AAAACGGCAT CCAGAACGGC 
TCTGGTACCC ACACTTGGTT TTTACGGAGG ATTCCAGGTA CACAGTACTC GCTGAGGAAT 
GAATATGTTG GAAACTTTGT CAATGGCATA CGCCAAGGGC AGGGGCAGTT TTATTATGCA 
AATGGTGCTA TGTATGATGG AGAGTGGAAG AATAACAAGA AACACGGACT GGGAAAGTTT 
GTGTTTAAGA ATGGGCAGAT TTATGTTGGA GAGTTTGTGG AGGATCAGAT AGCCGAGTAT 
CCGAATTTCA AGTACGATCG AGTGAATACT CCAGATCTAA GTGGCATCCG GACACAGAGT 
TCCATGAATT TGGCAGGAAG TAGTACATCC AGATACAATA CTGGCATTCC AAGTTTAGCA 
GGGTCTTATA TTGAGCTTGA CTTATCAGCA CTATTATACT CATTCCCGGA GGAGGACAGG 
CAAGAAGAAC TGAAGCAGGT GGAATATGCC GTTCTACGGA ACCTCACTGT CCTGCGTAAG 
ATCTACAAAT TGTACAGCCA GCTGGGAAGT GATGGCTCCC CTGACAATAC CCTTCTCATG 
ACAAAGCTGC AGTTCTGGAG GTTTCTGAAG GATTGCAAGT TTCATCACTA CAGTATCACA 
CTTTCGGAAA TGGACCGTAT CCTAACCGTA GAGAATACTG ATGCTGAAGA GGTTCATTCG 
CCCCATTCCA CAATGCTTCT TCGAACCTTT TTGACTAATA TTATCTACTT GGCATACCAC 
ATCTGTCAAA CAGAAAACCA AGAGAAGAAC ATGTCCCTTG CTGACTGCTT TACTAAAGTC 
ATGGTGCAGA ATATACTTCC CCATGCAACT AATATCAAAG GGTTTCTATT TCCTGATGCT 
CAGAAGGCTT CCTACGCCAT TTCTTATATT GAAAAGTCTT GGCATATCTA CAGAATCTAC 
TGCATGCCAA ACACTTTCCA CCCCTATGAA CCGACCATGA AAATGAGGCA CTTTCTGTTG 
CTAATGAATC TAACGTTTCT CGAGTTCTTT GAGGCGCTCC TTGGCTGTGC TTCCATATGT 
GTTACTGAAG AGCAGATAAA GCAGTCAGAG AGCGAATTGT TGAAGAGAGA AGAGTCTCAG 
GGGAGCTCGA TTGGGGAAGT CACAGCTTAT CTAAAAGAAG GCCAACCAGA GCATGCTTTT 
CAGCCCCAAG CTGACATACC GCAGGACCTC TTTCTGAGAA GCTCCATGAA CCTTAAGGAT 
AAGAGACAGA GCCCACCTAT TCCAGTCTCT TCTATAAAGA AGCAGCAGAG AGAGGCCAGG 
GCAGAGAAGT GGTTCCATCA GACTGACATA TTTTTCCTGA AGAAGTTCTT TCCAGCACAT 
GAACACAAAG AACGACTGAA GGAAGAAATT CCCAAAATCA GGGAGAAGCA GGCTGAAGAA 
GCCAGACGAC AGCAGATTAG AGAAGAGGAA GAAGCAAGGC TTAAAGCAGA AAGGGAGGCA 
GAAGAGGCCA AAAGGGTTGA ACAGGAGTTG TTGGAAAAGG CCATGGCTGC AATGGAAGCA 
GAACAGGCAG CAAAGGCACA AGAAGAAGAA CAGAAAAGTC CTCAGCCTCC AGTCACTCCT 
CATGATGACG CTATCATTAT CCCACCACCA TCTGGCACAA AGACTGCAAC TGCAAGCAAA 
AAGAAGAGAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826513.1
CDS	119..2533
Translation	MGKMKKKDSKKGERERSAQDTPEECSFKLSPVSITPSEEQHSHSSSMKSIPQEEHMKQTEPPVVTPATDVYEEPVLTQLIVEKYEGELLNGLYEGEGVAYFKDGNIYRGMFSEGMMHGKGKYTWKDGLTYEGDFYMNFPMGHGIYKWSDGSQYEGEVYKAIRHGHGIFTSSNQKVSYVGDWHKGTRHGKGVIYYNKEGTSWYEGDWISNKKEGWGVQCFISGNIYEGQWKNNIFHGMGKMRWLTSNEEYAGQWENGIQNGSGTHTWFLRRIPGTQYSLRNEYVGNFVNGIRQGQGQFYYANGAMYDGEWKNNKKHGLGKFVFKNGQIYVGEFVEDQIAEYPNFKYDRVNTPDLSGIRTQSSMNLAGSSTSRYNTGIPSLAGSYIELDLSALLYSFPEEDRQEELKQVEYAVLRNLTVLRKIYKLYSQLGSDGSPDNTLLMTKLQFWRFLKDCKFHHYSITLSEMDRILTVENTDAEEVHSPHSTMLLRTFLTNIIYLAYHICQTENQEKNMSLADCFTKVMVQNILPHATNIKGFLFPDAQKASYAISYIEKSWHIYRIYCMPNTFHPYEPTMKMRHFLLLMNLTFLEFFEALLGCASICVTEEQIKQSESELLKREESQGSSIGEVTAYLKEGQPEHAFQPQADIPQDLFLRSSMNLKDKRQSPPIPVSSIKKQQREARAEKWFHQTDIFFLKKFFPAHEHKERLKEEIPKIREKQAEEARRQQIREEEEARLKAEREAEEAKRVEQELLEKAMAAMEAEQAAKAQEEEQKSPQPPVTPHDDAIIIPPPSGTKTATASKKKRK

Target ORF information:

RefSeq Version	XM_012971059.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis radial spoke head 10 homolog B (Chlamydomonas) (rsph10b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971059.2

ORF Insert Sequence:

ATGGGAAAAA TGAAGAAAAA AGACAGCAAA AAGGGAGAGA GGGAGAGATC TGCACAAGAC 
ACCCCTGAGG AATGCAGCTT TAAGTTATCT CCAGTTTCCA TCACTCCATC TGAAGAACAA 
CATTCCCATT CCTCTTCCAT GAAATCCATT CCCCAGGAAG AACACATGAA GCAGACTGAA 
CCTCCTGTTG TGACTCCTGC AACAGATGTG TATGAAGAAC CTGTGCTAAC TCAACTTATT 
GTTGAAAAAT ATGAAGGAGA ATTGCTAAAT GGATTGTATG AAGGGGAAGG TGTTGCATAT 
TTTAAAGATG GAAATATTTA TAGAGGAATG TTTTCTGAAG GGATGATGCA TGGCAAAGGA 
AAATACACAT GGAAAGATGG ACTGACATAT GAGGGAGACT TTTACATGAA CTTTCCCATG 
GGACACGGTA TATATAAATG GTCTGATGGC AGCCAATATG AAGGAGAAGT GTATAAGGCA 
ATAAGACATG GGCATGGCAT TTTCACATCC AGTAATCAGA AGGTTTCGTA TGTTGGAGAC 
TGGCATAAAG GCACTCGGCA TGGGAAGGGA GTTATTTACT ATAATAAAGA GGGAACCTCA 
TGGTACGAAG GAGACTGGAT ATCTAACAAG AAGGAGGGCT GGGGAGTGCA GTGCTTTATC 
TCTGGCAACA TTTATGAAGG TCAATGGAAA AACAATATAT TTCATGGGAT GGGGAAAATG 
AGATGGCTGA CATCAAACGA AGAGTATGCA GGGCAGTGGG AAAACGGCAT CCAGAACGGC 
TCTGGTACCC ACACTTGGTT TTTACGGAGG ATTCCAGGTA CACAGTACTC GCTGAGGAAT 
GAATATGTTG GAAACTTTGT CAATGGCATA CGCCAAGGGC AGGGGCAGTT TTATTATGCA 
AATGGTGCTA TGTATGATGG AGAGTGGAAG AATAACAAGA AACACGGACT GGGAAAGTTT 
GTGTTTAAGA ATGGGCAGAT TTATGTTGGA GAGTTTGTGG AGGATCAGAT AGCCGAGTAT 
CCGAATTTCA AGTACGATCG AGTGAATACT CCAGATCTAA GTGGCATCCG GACACAGAGT 
TCCATGAATT TGGCAGGAAG TAGTACATCC AGATACAATA CTGGCATTCC AAGTTTAGCA 
GGGTCTTATA TTGAGCTTGA CTTATCAGCA CTATTATACT CATTCCCGGA GGAGGACAGG 
CAAGAAGAAC TGAAGCAGGT GGAATATGCC GTTCTACGGA ACCTCACTGT CCTGCGTAAG 
ATCTACAAAT TGTACAGCCA GCTGGGAAGT GATGGCTCCC CTGACAATAC CCTTCTCATG 
ACAAAGCTGC AGTTCTGGAG GTTTCTGAAG GATTGCAAGT TTCATCACTA CAGTATCACA 
CTTTCGGAAA TGGACCGTAT CCTAACCGTA GAGAATACTG ATGCTGAAGA GGTTCATTCG 
CCCCATTCCA CAATGCTTCT TCGAACCTTT TTGACTAATA TTATCTACTT GGCATACCAC 
ATCTGTCAAA CAGAAAACCA AGAGAAGAAC ATGTCCCTTG CTGACTGCTT TACTAAAGTC 
ATGGTGCAGA ATATACTTCC CCATGCAACT AATATCAAAG GGTTTCTATT TCCTGATGCT 
CAGAAGGCTT CCTACGCCAT TTCTTATATT GAAAAGTCTT GGCATATCTA CAGAATCTAC 
TGCATGCCAA ACACTTTCCA CCCCTATGAA CCGACCATGA AAATGAGGCA CTTTCTGTTG 
CTAATGAATC TAACGTTTCT CGAGTTCTTT GAGGCGCTCC TTGGCTGTGC TTCCATATGT 
GTTACTGAAG AGCAGATAAA GCAGTCAGAG AGCGAATTGT TGAAGAGAGA AGAGTCTCAG 
GGGAGCTCGA TTGGGGAAGT CACAGCTTAT CTAAAAGAAG GCCAACCAGA GCATGCTTTT 
CAGCCCCAAG CTGACATACC GCAGGACCTC TTTCTGAGAA GCTCCATGAA CCTTAAGGAT 
AAGAGACAGA GCCCACCTAT TCCAGTCTCT TCTATAAAGA AGCAGCAGAG AGAGGCCAGG 
GCAGAGAAGT GGTTCCATCA GACTGACATA TTTTTCCTGA AGAAGTTCTT TCCAGCACAT 
GAACACAAAG AACGACTGAA GGAAGAAATT CCCAAAATCA GGGAGAAGCA GGCTGAAGAA 
GCCAGACGAC AGCAGATTAG AGAAGAGGAA GAAGCAAGGC TTAAAGCAGA AAGGGAGGCA 
GAAGAGGCCA AAAGGGTTGA ACAGGAGTTG TTGGAAAAGG CCATGGCTGC AATGGAAGCA 
GAACAGGCAG CAAAGGCACA AGAAGAAGAA CAGAAAAGTC CTCAGCCTCC AGTCACTCCT 
CATGATGACG CTATCATTAT CCCACCACCA TCTGGCACAA AGACTGCAAC TGCAAGCAAA 
AAGAAGAGAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rsph10b ( XM_004917978.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004918035.1 Xenopus tropicalis radial spoke head 10 homolog B (Chlamydomonas) (rsph10b), transcript variant X1, mRNA.

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CloneID	OXa16354
Clone ID Related Accession (Same CDS sequence)	XM_004917978.4 , XM_004917978.3
Accession Version	XM_004917978.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	radial spoke head 10 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917978.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAAA TGAAGAAAAA AGACAGCAAA AAGGGAGAGA GGGAGAGATC TGCACAAGAC 
ACCCCTGAGG AATGCAGCTT TAAGTTATCT CCAGTTTCCA TCACTCCATC TGAAGAACAA 
CATTCCCATT CCTCTTCCAT GAAATCCATT CCCCAGGAAG AACACATGAA GCAGACTGAA 
CCTCCTGTTG TGACTCCTGC AACAGATGTG TATGAAGAAC CTGTGCTAAC TCAACTTATT 
GTTGAAAAAT ATGAAGGAGA ATTGCTAAAT GGATTGTATG AAGGGGAAGG TGTTGCATAT 
TTTAAAGATG GAAATATTTA TAGAGGAATG TTTTCTGAAG GGATGATGCA TGGCAAAGGA 
AAATACACAT GGAAAGATGG ACTGACATAT GAGGGAGACT TTTACATGAA CTTTCCCATG 
GGACACGGTA TATATAAATG GTCTGATGGC AGCCAATATG AAGGAGAAGT GTATAAGGCA 
ATAAGACATG GGCATGGCAT TTTCACATCC AGTAATCAGA AGGTTTCGTA TGTTGGAGAC 
TGGCATAAAG GCACTCGGCA TGGGAAGGGA GTTATTTACT ATAATAAAGA GGGAACCTCA 
TGGTACGAAG GAGACTGGAT ATCTAACAAG AAGGAGGGCT GGGGAGTGCA GTGCTTTATC 
TCTGGCAACA TTTATGAAGG TCAATGGAAA AACAATATAT TTCATGGGAT GGGGAAAATG 
AGATGGCTGA CATCAAACGA AGAGTATGCA GGGCAGTGGG AAAACGGCAT CCAGAACGGC 
TCTGGTACCC ACACTTGGTT TTTACGGAGG ATTCCAGGTA CACAGTACTC GCTGAGGAAT 
GAATATGTTG GAAACTTTGT CAATGGCATA CGCCAAGGGC AGGGGCAGTT TTATTATGCA 
AATGGTGCTA TGTATGATGG AGAGTGGAAG AATAACAAGA AACACGGACT GGGAAAGTTT 
GTGTTTAAGA ATGGGCAGAT TTATGTTGGA GAGTTTGTGG AGGATCAGAT AGCCGAGTAT 
CCGAATTTCA AGTACGATCG AGTGAATACT CCAGATCTAA GTGGCATCCG GACACAGAGT 
TCCATGAATT TGGCAGGAAG TAGTACATCC AGATACAATA CTGGCATTCC AAGTTTAGCA 
GGGTCTTATA TTGAGCTTGA CTTATCAGCA CTATTATACT CATTCCCGGA GGAGGACAGG 
CAAGAAGAAC TGAAGCAGGT GGAATATGCC GTTCTACGGA ACCTCACTGT CCTGCGTAAG 
ATCTACAAAT TGTACAGCCA GCTGGGAAGT GATGGCTCCC CTGACAATAC CCTTCTCATG 
ACAAAGCTGC AGTTCTGGAG GTTTCTGAAG GATTGCAAGT TTCATCACTA CAGTATCACA 
CTTTCGGAAA TGGACCGTAT CCTAACCGTA GAGAATACTG ATGCTGAAGA GGTTCATTCG 
CCCCATTCCA CAATGCTTCT TCGAACCTTT TTGACTAATA TTATCTACTT GGCATACCAC 
ATCTGTCAAA CAGAAAACCA AGAGAAGAAC ATGTCCCTTG CTGACTGCTT TACTAAAGTC 
ATGGTGCAGA ATATACTTCC CCATGCAACT AATATCAAAG GGTTTCTATT TCCTGATGCT 
CAGAAGGCTT CCTACGCCAT TTCTTATATT GAAAAGTCTT GGCATATCTA CAGAATCTAC 
TGCATGCCAA ACACTTTCCA CCCCTATGAA CCGACCATGA AAATGAGGCA CTTTCTGTTG 
CTAATGAATG ATTTAAAAAT CACAGGACTG ACAGCTACAA ATATTGTTGA CATTTTGGCA 
GCAGATGACC CATGTGTGCG TATTGACCAG GAAGTGAACC TGGAATTGGA GCTAACGTTT 
CTCGAGTTCT TTGAGGCGCT CCTTGGCTGT GCTTCCATAT GTGTTACTGA AGAGCAGATA 
AAGCAGTCAG AGAGCGAATT GTTGAAGAGA GAAGAGTCTC AGGGGAGCTC GATTGGGGAA 
GTCACAGCTT ATCTAAAAGA AGGCCAACCA GAGCATGCTT TTCAGCCCCA AGCTGACATA 
CCGCAGGACC TCTTTCTGAG AAGCTCCATG AACCTTAAGG ATAAGAGACA GAGCCCACCT 
ATTCCAGTCT CTTCTATAAA GAAGCAGCAG AGAGAGGCCA GGGCAGAGAA GTGGTTCCAT 
CAGACTGACA TATTTTTCCT GAAGAAGTTC TTTCCAGCAC ATGAACACAA AGAACGACTG 
AAGGAAGAAA TTCCCAAAAT CAGGGAGAAG CAGGCTGAAG AAGCCAGACG ACAGCAGATT 
AGAGAAGAGG AAGAAGCAAG GCTTAAAGCA GAAAGGGAGG CAGAAGAGGC CAAAAGGGTT 
GAACAGGAGT TGTTGGAAAA GGCCATGGCT GCAATGGAAG CAGAACAGGC AGCAAAGGCA 
CAAGAAGAAG AACAGAAAAG TCCTCAGCCT CCAGTCACTC CTCATGATGA CGCTATCATT 
ATCCCACCAC CATCTGGCAC AAAGACTGCA ACTGCAAGCA AAAAGAAGAG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004918035.1
CDS	146..2662
Translation	MGKMKKKDSKKGERERSAQDTPEECSFKLSPVSITPSEEQHSHSSSMKSIPQEEHMKQTEPPVVTPATDVYEEPVLTQLIVEKYEGELLNGLYEGEGVAYFKDGNIYRGMFSEGMMHGKGKYTWKDGLTYEGDFYMNFPMGHGIYKWSDGSQYEGEVYKAIRHGHGIFTSSNQKVSYVGDWHKGTRHGKGVIYYNKEGTSWYEGDWISNKKEGWGVQCFISGNIYEGQWKNNIFHGMGKMRWLTSNEEYAGQWENGIQNGSGTHTWFLRRIPGTQYSLRNEYVGNFVNGIRQGQGQFYYANGAMYDGEWKNNKKHGLGKFVFKNGQIYVGEFVEDQIAEYPNFKYDRVNTPDLSGIRTQSSMNLAGSSTSRYNTGIPSLAGSYIELDLSALLYSFPEEDRQEELKQVEYAVLRNLTVLRKIYKLYSQLGSDGSPDNTLLMTKLQFWRFLKDCKFHHYSITLSEMDRILTVENTDAEEVHSPHSTMLLRTFLTNIIYLAYHICQTENQEKNMSLADCFTKVMVQNILPHATNIKGFLFPDAQKASYAISYIEKSWHIYRIYCMPNTFHPYEPTMKMRHFLLLMNDLKITGLTATNIVDILAADDPCVRIDQEVNLELELTFLEFFEALLGCASICVTEEQIKQSESELLKREESQGSSIGEVTAYLKEGQPEHAFQPQADIPQDLFLRSSMNLKDKRQSPPIPVSSIKKQQREARAEKWFHQTDIFFLKKFFPAHEHKERLKEEIPKIREKQAEEARRQQIREEEEARLKAEREAEEAKRVEQELLEKAMAAMEAEQAAKAQEEEQKSPQPPVTPHDDAIIIPPPSGTKTATASKKKRK

Target ORF information:

RefSeq Version	XM_004917978.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis radial spoke head 10 homolog B (Chlamydomonas) (rsph10b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917978.4

ORF Insert Sequence:

ATGGGAAAAA TGAAGAAAAA AGACAGCAAA AAGGGAGAGA GGGAGAGATC TGCACAAGAC 
ACCCCTGAGG AATGCAGCTT TAAGTTATCT CCAGTTTCCA TCACTCCATC TGAAGAACAA 
CATTCCCATT CCTCTTCCAT GAAATCCATT CCCCAGGAAG AACACATGAA GCAGACTGAA 
CCTCCTGTTG TGACTCCTGC AACAGATGTG TATGAAGAAC CTGTGCTAAC TCAACTTATT 
GTTGAAAAAT ATGAAGGAGA ATTGCTAAAT GGATTGTATG AAGGGGAAGG TGTTGCATAT 
TTTAAAGATG GAAATATTTA TAGAGGAATG TTTTCTGAAG GGATGATGCA TGGCAAAGGA 
AAATACACAT GGAAAGATGG ACTGACATAT GAGGGAGACT TTTACATGAA CTTTCCCATG 
GGACACGGTA TATATAAATG GTCTGATGGC AGCCAATATG AAGGAGAAGT GTATAAGGCA 
ATAAGACATG GGCATGGCAT TTTCACATCC AGTAATCAGA AGGTTTCGTA TGTTGGAGAC 
TGGCATAAAG GCACTCGGCA TGGGAAGGGA GTTATTTACT ATAATAAAGA GGGAACCTCA 
TGGTACGAAG GAGACTGGAT ATCTAACAAG AAGGAGGGCT GGGGAGTGCA GTGCTTTATC 
TCTGGCAACA TTTATGAAGG TCAATGGAAA AACAATATAT TTCATGGGAT GGGGAAAATG 
AGATGGCTGA CATCAAACGA AGAGTATGCA GGGCAGTGGG AAAACGGCAT CCAGAACGGC 
TCTGGTACCC ACACTTGGTT TTTACGGAGG ATTCCAGGTA CACAGTACTC GCTGAGGAAT 
GAATATGTTG GAAACTTTGT CAATGGCATA CGCCAAGGGC AGGGGCAGTT TTATTATGCA 
AATGGTGCTA TGTATGATGG AGAGTGGAAG AATAACAAGA AACACGGACT GGGAAAGTTT 
GTGTTTAAGA ATGGGCAGAT TTATGTTGGA GAGTTTGTGG AGGATCAGAT AGCCGAGTAT 
CCGAATTTCA AGTACGATCG AGTGAATACT CCAGATCTAA GTGGCATCCG GACACAGAGT 
TCCATGAATT TGGCAGGAAG TAGTACATCC AGATACAATA CTGGCATTCC AAGTTTAGCA 
GGGTCTTATA TTGAGCTTGA CTTATCAGCA CTATTATACT CATTCCCGGA GGAGGACAGG 
CAAGAAGAAC TGAAGCAGGT GGAATATGCC GTTCTACGGA ACCTCACTGT CCTGCGTAAG 
ATCTACAAAT TGTACAGCCA GCTGGGAAGT GATGGCTCCC CTGACAATAC CCTTCTCATG 
ACAAAGCTGC AGTTCTGGAG GTTTCTGAAG GATTGCAAGT TTCATCACTA CAGTATCACA 
CTTTCGGAAA TGGACCGTAT CCTAACCGTA GAGAATACTG ATGCTGAAGA GGTTCATTCG 
CCCCATTCCA CAATGCTTCT TCGAACCTTT TTGACTAATA TTATCTACTT GGCATACCAC 
ATCTGTCAAA CAGAAAACCA AGAGAAGAAC ATGTCCCTTG CTGACTGCTT TACTAAAGTC 
ATGGTGCAGA ATATACTTCC CCATGCAACT AATATCAAAG GGTTTCTATT TCCTGATGCT 
CAGAAGGCTT CCTACGCCAT TTCTTATATT GAAAAGTCTT GGCATATCTA CAGAATCTAC 
TGCATGCCAA ACACTTTCCA CCCCTATGAA CCGACCATGA AAATGAGGCA CTTTCTGTTG 
CTAATGAATG ATTTAAAAAT CACAGGACTG ACAGCTACAA ATATTGTTGA CATTTTGGCA 
GCAGATGACC CATGTGTGCG TATTGACCAG GAAGTGAACC TGGAATTGGA GCTAACGTTT 
CTCGAGTTCT TTGAGGCGCT CCTTGGCTGT GCTTCCATAT GTGTTACTGA AGAGCAGATA 
AAGCAGTCAG AGAGCGAATT GTTGAAGAGA GAAGAGTCTC AGGGGAGCTC GATTGGGGAA 
GTCACAGCTT ATCTAAAAGA AGGCCAACCA GAGCATGCTT TTCAGCCCCA AGCTGACATA 
CCGCAGGACC TCTTTCTGAG AAGCTCCATG AACCTTAAGG ATAAGAGACA GAGCCCACCT 
ATTCCAGTCT CTTCTATAAA GAAGCAGCAG AGAGAGGCCA GGGCAGAGAA GTGGTTCCAT 
CAGACTGACA TATTTTTCCT GAAGAAGTTC TTTCCAGCAC ATGAACACAA AGAACGACTG 
AAGGAAGAAA TTCCCAAAAT CAGGGAGAAG CAGGCTGAAG AAGCCAGACG ACAGCAGATT 
AGAGAAGAGG AAGAAGCAAG GCTTAAAGCA GAAAGGGAGG CAGAAGAGGC CAAAAGGGTT 
GAACAGGAGT TGTTGGAAAA GGCCATGGCT GCAATGGAAG CAGAACAGGC AGCAAAGGCA 
CAAGAAGAAG AACAGAAAAG TCCTCAGCCT CCAGTCACTC CTCATGATGA CGCTATCATT 
ATCCCACCAC CATCTGGCAC AAAGACTGCA ACTGCAAGCA AAAAGAAGAG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rsph10b ( XM_002931975.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932021.2 Xenopus tropicalis radial spoke head 10 homolog B (Chlamydomonas) (rsph10b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa16355
Clone ID Related Accession (Same CDS sequence)	XM_002931975.4 , XM_002931975.5
Accession Version	XM_002931975.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	radial spoke head 10 homolog B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002931975.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAAA TGAAGAAAAA AGACAGCAAA AAGGGAGAGA GGGAGAGATC TGCACAAGAC 
ACCCCTGAGG AATGCAGCTT TAAGTTATCT CCAGTTTCCA TCACTCCATC TGAAGAACAA 
CATTCCCATT CCTCTTCCAT GAAATCCATT CCCCAGGAAG AACACATGAA GCAGACTGAA 
CCTCCTGTTG TGACTCCTGC AACAGATGTG TATGAAGAAC CTGTGCTAAC TCAACTTATT 
GTTGAAAAAT ATGAAGGAGA ATTGCTAAAT GGATTGTATG AAGGGGAAGG TGTTGCATAT 
TTTAAAGATG GAAATATTTA TAGAGGAATG TTTTCTGAAG GGATGATGCA TGGCAAAGGA 
AAATACACAT GGAAAGATGG ACTGACATAT GAGGGAGACT TTTACATGAA CTTTCCCATG 
GGACACGGTA TATATAAATG GTCTGATGGC AGCCAATATG AAGGAGAAGT GTATAAGGCA 
ATAAGACATG GGCATGGCAT TTTCACATCC AGTAATCAGA AGGTTTCGTA TGTTGGAGAC 
TGGCATAAAG GCACTCGGCA TGGGAAGGGA GTTATTTACT ATAATAAAGA GGGAACCTCA 
TGGTACGAAG GAGACTGGAT ATCTAACAAG AAGGAGGGCT GGGGAGTGCA GTGCTTTATC 
TCTGGCAACA TTTATGAAGG TCAATGGAAA AACAATATAT TTCATGGGAT GGGGAAAATG 
AGATGGCTGA CATCAAACGA AGAGTATGCA GGGCAGTGGG AAAACGGCAT CCAGAACGGC 
TCTGGTACCC ACACTTGGTT TTTACGGAGG ATTCCAGGTA CACAGTACTC GCTGAGGAAT 
GAATATGTTG GAAACTTTGT CAATGGCATA CGCCAAGGGC AGGGGCAGTT TTATTATGCA 
AATGGTGCTA TGTATGATGG AGAGTGGAAG AATAACAAGA AACACGGACT GGGAAAGTTT 
GTGTTTAAGA ATGGGCAGAT TTATGTTGGA GAGTTTGTGG AGGATCAGAT AGCCGAGTAT 
CCGAATTTCA AGTACGATCG AGTGAATACT CCAGATCTAA GTGGCATCCG GACACAGAGT 
TCCATGAATT TGGGAAGTAG TACATCCAGA TACAATACTG GCATTCCAAG TTTAGCAGGG 
TCTTATATTG AGCTTGACTT ATCAGCACTA TTATACTCAT TCCCGGAGGA GGACAGGCAA 
GAAGAACTGA AGCAGGTGGA ATATGCCGTT CTACGGAACC TCACTGTCCT GCGTAAGATC 
TACAAATTGT ACAGCCAGCT GGGAAGTGAT GGCTCCCCTG ACAATACCCT TCTCATGACA 
AAGCTGCAGT TCTGGAGGTT TCTGAAGGAT TGCAAGTTTC ATCACTACAG TATCACACTT 
TCGGAAATGG ACCGTATCCT AACCGTAGAG AATACTGATG CTGAAGAGGT TCATTCGCCC 
CATTCCACAA TGCTTCTTCG AACCTTTTTG ACTAATATTA TCTACTTGGC ATACCACATC 
TGTCAAACAG AAAACCAAGA GAAGAACATG TCCCTTGCTG ACTGCTTTAC TAAAGTCATG 
GTGCAGAATA TACTTCCCCA TGCAACTAAT ATCAAAGGGT TTCTATTTCC TGATGCTCAG 
AAGGCTTCCT ACGCCATTTC TTATATTGAA AAGTCTTGGC ATATCTACAG AATCTACTGC 
ATGCCAAACA CTTTCCACCC CTATGAACCG ACCATGAAAA TGAGGCACTT TCTGTTGCTA 
ATGAATGATT TAAAAATCAC AGGACTGACA GCTACAAATA TTGTTGACAT TTTGGCAGCA 
GATGACCCAT GTGTGCGTAT TGACCAGGAA GTGAACCTGG AATTGGAGCT AACGTTTCTC 
GAGTTCTTTG AGGCGCTCCT TGGCTGTGCT TCCATATGTG TTACTGAAGA GCAGATAAAG 
CAGTCAGAGA GCGAATTGTT GAAGAGAGAA GAGTCTCAGG GGAGCTCGAT TGGGGAAGTC 
ACAGCTTATC TAAAAGAAGG CCAACCAGAG CATGCTTTTC AGCCCCAAGC TGACATACCG 
CAGGACCTCT TTCTGAGAAG CTCCATGAAC CTTAAGGATA AGAGACAGAG CCCACCTATT 
CCAGTCTCTT CTATAAAGAA GCAGCAGAGA GAGGCCAGGG CAGAGAAGTG GTTCCATCAG 
ACTGACATAT TTTTCCTGAA GAAGTTCTTT CCAGCACATG AACACAAAGA ACGACTGAAG 
GAAGAAATTC CCAAAATCAG GGAGAAGCAG GCTGAAGAAG CCAGACGACA GCAGATTAGA 
GAAGAGGAAG AAGCAAGGCT TAAAGCAGAA AGGGAGGCAG AAGAGGCCAA AAGGGTTGAA 
CAGGAGTTGT TGGAAAAGGC CATGGCTGCA ATGGAAGCAG AACAGGCAGC AAAGGCACAA 
GAAGAAGAAC AGAAAAGTCC TCAGCCTCCA GTCACTCCTC ATGATGACGC TATCATTATC 
CCACCACCAT CTGGCACAAA GACTGCAACT GCAAGCAAAA AGAAGAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932021.2
CDS	146..2659
Translation	MGKMKKKDSKKGERERSAQDTPEECSFKLSPVSITPSEEQHSHSSSMKSIPQEEHMKQTEPPVVTPATDVYEEPVLTQLIVEKYEGELLNGLYEGEGVAYFKDGNIYRGMFSEGMMHGKGKYTWKDGLTYEGDFYMNFPMGHGIYKWSDGSQYEGEVYKAIRHGHGIFTSSNQKVSYVGDWHKGTRHGKGVIYYNKEGTSWYEGDWISNKKEGWGVQCFISGNIYEGQWKNNIFHGMGKMRWLTSNEEYAGQWENGIQNGSGTHTWFLRRIPGTQYSLRNEYVGNFVNGIRQGQGQFYYANGAMYDGEWKNNKKHGLGKFVFKNGQIYVGEFVEDQIAEYPNFKYDRVNTPDLSGIRTQSSMNLGSSTSRYNTGIPSLAGSYIELDLSALLYSFPEEDRQEELKQVEYAVLRNLTVLRKIYKLYSQLGSDGSPDNTLLMTKLQFWRFLKDCKFHHYSITLSEMDRILTVENTDAEEVHSPHSTMLLRTFLTNIIYLAYHICQTENQEKNMSLADCFTKVMVQNILPHATNIKGFLFPDAQKASYAISYIEKSWHIYRIYCMPNTFHPYEPTMKMRHFLLLMNDLKITGLTATNIVDILAADDPCVRIDQEVNLELELTFLEFFEALLGCASICVTEEQIKQSESELLKREESQGSSIGEVTAYLKEGQPEHAFQPQADIPQDLFLRSSMNLKDKRQSPPIPVSSIKKQQREARAEKWFHQTDIFFLKKFFPAHEHKERLKEEIPKIREKQAEEARRQQIREEEEARLKAEREAEEAKRVEQELLEKAMAAMEAEQAAKAQEEEQKSPQPPVTPHDDAIIIPPPSGTKTATASKKKRK

Target ORF information:

RefSeq Version	XM_002931975.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis radial spoke head 10 homolog B (Chlamydomonas) (rsph10b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931975.5

ORF Insert Sequence:

ATGGGAAAAA TGAAGAAAAA AGACAGCAAA AAGGGAGAGA GGGAGAGATC TGCACAAGAC 
ACCCCTGAGG AATGCAGCTT TAAGTTATCT CCAGTTTCCA TCACTCCATC TGAAGAACAA 
CATTCCCATT CCTCTTCCAT GAAATCCATT CCCCAGGAAG AACACATGAA GCAGACTGAA 
CCTCCTGTTG TGACTCCTGC AACAGATGTG TATGAAGAAC CTGTGCTAAC TCAACTTATT 
GTTGAAAAAT ATGAAGGAGA ATTGCTAAAT GGATTGTATG AAGGGGAAGG TGTTGCATAT 
TTTAAAGATG GAAATATTTA TAGAGGAATG TTTTCTGAAG GGATGATGCA TGGCAAAGGA 
AAATACACAT GGAAAGATGG ACTGACATAT GAGGGAGACT TTTACATGAA CTTTCCCATG 
GGACACGGTA TATATAAATG GTCTGATGGC AGCCAATATG AAGGAGAAGT GTATAAGGCA 
ATAAGACATG GGCATGGCAT TTTCACATCC AGTAATCAGA AGGTTTCGTA TGTTGGAGAC 
TGGCATAAAG GCACTCGGCA TGGGAAGGGA GTTATTTACT ATAATAAAGA GGGAACCTCA 
TGGTACGAAG GAGACTGGAT ATCTAACAAG AAGGAGGGCT GGGGAGTGCA GTGCTTTATC 
TCTGGCAACA TTTATGAAGG TCAATGGAAA AACAATATAT TTCATGGGAT GGGGAAAATG 
AGATGGCTGA CATCAAACGA AGAGTATGCA GGGCAGTGGG AAAACGGCAT CCAGAACGGC 
TCTGGTACCC ACACTTGGTT TTTACGGAGG ATTCCAGGTA CACAGTACTC GCTGAGGAAT 
GAATATGTTG GAAACTTTGT CAATGGCATA CGCCAAGGGC AGGGGCAGTT TTATTATGCA 
AATGGTGCTA TGTATGATGG AGAGTGGAAG AATAACAAGA AACACGGACT GGGAAAGTTT 
GTGTTTAAGA ATGGGCAGAT TTATGTTGGA GAGTTTGTGG AGGATCAGAT AGCCGAGTAT 
CCGAATTTCA AGTACGATCG AGTGAATACT CCAGATCTAA GTGGCATCCG GACACAGAGT 
TCCATGAATT TGGGAAGTAG TACATCCAGA TACAATACTG GCATTCCAAG TTTAGCAGGG 
TCTTATATTG AGCTTGACTT ATCAGCACTA TTATACTCAT TCCCGGAGGA GGACAGGCAA 
GAAGAACTGA AGCAGGTGGA ATATGCCGTT CTACGGAACC TCACTGTCCT GCGTAAGATC 
TACAAATTGT ACAGCCAGCT GGGAAGTGAT GGCTCCCCTG ACAATACCCT TCTCATGACA 
AAGCTGCAGT TCTGGAGGTT TCTGAAGGAT TGCAAGTTTC ATCACTACAG TATCACACTT 
TCGGAAATGG ACCGTATCCT AACCGTAGAG AATACTGATG CTGAAGAGGT TCATTCGCCC 
CATTCCACAA TGCTTCTTCG AACCTTTTTG ACTAATATTA TCTACTTGGC ATACCACATC 
TGTCAAACAG AAAACCAAGA GAAGAACATG TCCCTTGCTG ACTGCTTTAC TAAAGTCATG 
GTGCAGAATA TACTTCCCCA TGCAACTAAT ATCAAAGGGT TTCTATTTCC TGATGCTCAG 
AAGGCTTCCT ACGCCATTTC TTATATTGAA AAGTCTTGGC ATATCTACAG AATCTACTGC 
ATGCCAAACA CTTTCCACCC CTATGAACCG ACCATGAAAA TGAGGCACTT TCTGTTGCTA 
ATGAATGATT TAAAAATCAC AGGACTGACA GCTACAAATA TTGTTGACAT TTTGGCAGCA 
GATGACCCAT GTGTGCGTAT TGACCAGGAA GTGAACCTGG AATTGGAGCT AACGTTTCTC 
GAGTTCTTTG AGGCGCTCCT TGGCTGTGCT TCCATATGTG TTACTGAAGA GCAGATAAAG 
CAGTCAGAGA GCGAATTGTT GAAGAGAGAA GAGTCTCAGG GGAGCTCGAT TGGGGAAGTC 
ACAGCTTATC TAAAAGAAGG CCAACCAGAG CATGCTTTTC AGCCCCAAGC TGACATACCG 
CAGGACCTCT TTCTGAGAAG CTCCATGAAC CTTAAGGATA AGAGACAGAG CCCACCTATT 
CCAGTCTCTT CTATAAAGAA GCAGCAGAGA GAGGCCAGGG CAGAGAAGTG GTTCCATCAG 
ACTGACATAT TTTTCCTGAA GAAGTTCTTT CCAGCACATG AACACAAAGA ACGACTGAAG 
GAAGAAATTC CCAAAATCAG GGAGAAGCAG GCTGAAGAAG CCAGACGACA GCAGATTAGA 
GAAGAGGAAG AAGCAAGGCT TAAAGCAGAA AGGGAGGCAG AAGAGGCCAA AAGGGTTGAA 
CAGGAGTTGT TGGAAAAGGC CATGGCTGCA ATGGAAGCAG AACAGGCAGC AAAGGCACAA 
GAAGAAGAAC AGAAAAGTCC TCAGCCTCCA GTCACTCCTC ATGATGACGC TATCATTATC 
CCACCACCAT CTGGCACAAA GACTGCAACT GCAAGCAAAA AGAAGAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rsph10b ( XM_012971058.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826512.1 Xenopus tropicalis radial spoke head 10 homolog B (Chlamydomonas) (rsph10b), transcript variant X3, mRNA.

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CloneID	OXa28952
Clone ID Related Accession (Same CDS sequence)	XM_012971058.2 , XM_012971058.3
Accession Version	XM_012971058.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	radial spoke head 10 homolog B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971058.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAAA TGAAGAAAAA AGACAGCAAA AAGGGAGAGA GGGAGAGATC TGCACAAGAC 
ACCCCTGAGG AATGCAGCTT TAAGTTATCT CCAGTTTCCA TCACTCCATC TGAAGAACAA 
CATTCCCATT CCTCTTCCAT GAAATCCATT CCCCAGGAAG AACACATGAA GCAGACTGAA 
CCTCCTGTTG TGACTCCTGC AACAGATGTG TATGAAGAAC CTGTGCTAAC TCAACTTATT 
GTTGAAAAAT ATGAAGGAGA ATTGCTAAAT GGATTGTATG AAGGGGAAGG TGTTGCATAT 
TTTAAAGATG GAAATATTTA TAGAGGAATG TTTTCTGAAG GGATGATGCA TGGCAAAGGA 
AAATACACAT GGAAAGATGG ACTGACATAT GAGGGAGACT TTTACATGAA CTTTCCCATG 
GGACACGGTA TATATAAATG GTCTGATGGC AGCCAATATG AAGGAGAAGT GTATAAGGCA 
ATAAGACATG GGCATGGCAT TTTCACATCC AGTAATCAGA AGGTTTCGTA TGTTGGAGAC 
TGGCATAAAG GCACTCGGCA TGGGAAGGGA GTTATTTACT ATAATAAAGA GGGAACCTCA 
TGGTACGAAG GAGACTGGAT ATCTAACAAG AAGGAGGGCT GGGGAGTGCA GTGCTTTATC 
TCTGGCAACA TTTATGAAGG TCAATGGAAA AACAATATAT TTCATGGGAT GGGGAAAATG 
AGATGGCTGA CATCAAACGA AGAGTATGCA GGGCAGTGGG AAAACGGCAT CCAGAACGGC 
TCTGGTACCC ACACTTGGTT TTTACGGAGG ATTCCAGGTA CACAGTACTC GCTGAGGAAT 
GAATATGTTG GAAACTTTGT CAATGGCATA CGCCAAGGGC AGGGGCAGTT TTATTATGCA 
AATGGTGCTA TGTATGATGG AGAGTGGAAG AATAACAAGA AACACGGACT GGGAAAGTTT 
GTGTTTAAGA ATGGGCAGAT TTATGTTGGA GAGTTTGTGG AGGATCAGAT AGCCGAGTAT 
CCGAATTTCA AGTACGATCG AGTGAATACT CCAGATCTAA GTGGCATCCG GACACAGAGT 
TCCATGAATT TGGCAGGAAG TAGTACATCC AGATACAATA CTGGCATTCC AAGTTTAGCA 
GGGTCTTATA TTGAGCTTGA CTTATCAGCA CTATTATACT CATTCCCGGA GGAGGACAGG 
CAAGAAGAAC TGAAGCAGGT GGAATATGCC GTTCTACGGA ACCTCACTGT CCTGCGTAAG 
ATCTACAAAT TGTACAGCCA GCTGGGAAGT GATGGCTCCC CTGACAATAC CCTTCTCATG 
ACAAAGCTGC AGTTCTGGAG GTTTCTGAAG GATTGCAAGT TTCATCACTA CAGTATCACA 
CTTTCGGAAA TGGACCGTAT CCTAACCGAG AATACTGATG CTGAAGAGGT TCATTCGCCC 
CATTCCACAA TGCTTCTTCG AACCTTTTTG ACTAATATTA TCTACTTGGC ATACCACATC 
TGTCAAACAG AAAACCAAGA GAAGAACATG TCCCTTGCTG ACTGCTTTAC TAAAGTCATG 
GTGCAGAATA TACTTCCCCA TGCAACTAAT ATCAAAGGGT TTCTATTTCC TGATGCTCAG 
AAGGCTTCCT ACGCCATTTC TTATATTGAA AAGTCTTGGC ATATCTACAG AATCTACTGC 
ATGCCAAACA CTTTCCACCC CTATGAACCG ACCATGAAAA TGAGGCACTT TCTGTTGCTA 
ATGAATGATT TAAAAATCAC AGGACTGACA GCTACAAATA TTGTTGACAT TTTGGCAGCA 
GATGACCCAT GTGTGCGTAT TGACCAGGAA GTGAACCTGG AATTGGAGCT AACGTTTCTC 
GAGTTCTTTG AGGCGCTCCT TGGCTGTGCT TCCATATGTG TTACTGAAGA GCAGATAAAG 
CAGTCAGAGA GCGAATTGTT GAAGAGAGAA GAGTCTCAGG GGAGCTCGAT TGGGGAAGTC 
ACAGCTTATC TAAAAGAAGG CCAACCAGAG CATGCTTTTC AGCCCCAAGC TGACATACCG 
CAGGACCTCT TTCTGAGAAG CTCCATGAAC CTTAAGGATA AGAGACAGAG CCCACCTATT 
CCAGTCTCTT CTATAAAGAA GCAGCAGAGA GAGGCCAGGG CAGAGAAGTG GTTCCATCAG 
ACTGACATAT TTTTCCTGAA GAAGTTCTTT CCAGCACATG AACACAAAGA ACGACTGAAG 
GAAGAAATTC CCAAAATCAG GGAGAAGCAG GCTGAAGAAG CCAGACGACA GCAGATTAGA 
GAAGAGGAAG AAGCAAGGCT TAAAGCAGAA AGGGAGGCAG AAGAGGCCAA AAGGGTTGAA 
CAGGAGTTGT TGGAAAAGGC CATGGCTGCA ATGGAAGCAG AACAGGCAGC AAAGGCACAA 
GAAGAAGAAC AGAAAAGTCC TCAGCCTCCA GTCACTCCTC ATGATGACGC TATCATTATC 
CCACCACCAT CTGGCACAAA GACTGCAACT GCAAGCAAAA AGAAGAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826512.1
CDS	146..2659
Translation	MGKMKKKDSKKGERERSAQDTPEECSFKLSPVSITPSEEQHSHSSSMKSIPQEEHMKQTEPPVVTPATDVYEEPVLTQLIVEKYEGELLNGLYEGEGVAYFKDGNIYRGMFSEGMMHGKGKYTWKDGLTYEGDFYMNFPMGHGIYKWSDGSQYEGEVYKAIRHGHGIFTSSNQKVSYVGDWHKGTRHGKGVIYYNKEGTSWYEGDWISNKKEGWGVQCFISGNIYEGQWKNNIFHGMGKMRWLTSNEEYAGQWENGIQNGSGTHTWFLRRIPGTQYSLRNEYVGNFVNGIRQGQGQFYYANGAMYDGEWKNNKKHGLGKFVFKNGQIYVGEFVEDQIAEYPNFKYDRVNTPDLSGIRTQSSMNLAGSSTSRYNTGIPSLAGSYIELDLSALLYSFPEEDRQEELKQVEYAVLRNLTVLRKIYKLYSQLGSDGSPDNTLLMTKLQFWRFLKDCKFHHYSITLSEMDRILTENTDAEEVHSPHSTMLLRTFLTNIIYLAYHICQTENQEKNMSLADCFTKVMVQNILPHATNIKGFLFPDAQKASYAISYIEKSWHIYRIYCMPNTFHPYEPTMKMRHFLLLMNDLKITGLTATNIVDILAADDPCVRIDQEVNLELELTFLEFFEALLGCASICVTEEQIKQSESELLKREESQGSSIGEVTAYLKEGQPEHAFQPQADIPQDLFLRSSMNLKDKRQSPPIPVSSIKKQQREARAEKWFHQTDIFFLKKFFPAHEHKERLKEEIPKIREKQAEEARRQQIREEEEARLKAEREAEEAKRVEQELLEKAMAAMEAEQAAKAQEEEQKSPQPPVTPHDDAIIIPPPSGTKTATASKKKRK

Target ORF information:

RefSeq Version	XM_012971058.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis radial spoke head 10 homolog B (Chlamydomonas) (rsph10b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971058.3

ORF Insert Sequence:

ATGGGAAAAA TGAAGAAAAA AGACAGCAAA AAGGGAGAGA GGGAGAGATC TGCACAAGAC 
ACCCCTGAGG AATGCAGCTT TAAGTTATCT CCAGTTTCCA TCACTCCATC TGAAGAACAA 
CATTCCCATT CCTCTTCCAT GAAATCCATT CCCCAGGAAG AACACATGAA GCAGACTGAA 
CCTCCTGTTG TGACTCCTGC AACAGATGTG TATGAAGAAC CTGTGCTAAC TCAACTTATT 
GTTGAAAAAT ATGAAGGAGA ATTGCTAAAT GGATTGTATG AAGGGGAAGG TGTTGCATAT 
TTTAAAGATG GAAATATTTA TAGAGGAATG TTTTCTGAAG GGATGATGCA TGGCAAAGGA 
AAATACACAT GGAAAGATGG ACTGACATAT GAGGGAGACT TTTACATGAA CTTTCCCATG 
GGACACGGTA TATATAAATG GTCTGATGGC AGCCAATATG AAGGAGAAGT GTATAAGGCA 
ATAAGACATG GGCATGGCAT TTTCACATCC AGTAATCAGA AGGTTTCGTA TGTTGGAGAC 
TGGCATAAAG GCACTCGGCA TGGGAAGGGA GTTATTTACT ATAATAAAGA GGGAACCTCA 
TGGTACGAAG GAGACTGGAT ATCTAACAAG AAGGAGGGCT GGGGAGTGCA GTGCTTTATC 
TCTGGCAACA TTTATGAAGG TCAATGGAAA AACAATATAT TTCATGGGAT GGGGAAAATG 
AGATGGCTGA CATCAAACGA AGAGTATGCA GGGCAGTGGG AAAACGGCAT CCAGAACGGC 
TCTGGTACCC ACACTTGGTT TTTACGGAGG ATTCCAGGTA CACAGTACTC GCTGAGGAAT 
GAATATGTTG GAAACTTTGT CAATGGCATA CGCCAAGGGC AGGGGCAGTT TTATTATGCA 
AATGGTGCTA TGTATGATGG AGAGTGGAAG AATAACAAGA AACACGGACT GGGAAAGTTT 
GTGTTTAAGA ATGGGCAGAT TTATGTTGGA GAGTTTGTGG AGGATCAGAT AGCCGAGTAT 
CCGAATTTCA AGTACGATCG AGTGAATACT CCAGATCTAA GTGGCATCCG GACACAGAGT 
TCCATGAATT TGGCAGGAAG TAGTACATCC AGATACAATA CTGGCATTCC AAGTTTAGCA 
GGGTCTTATA TTGAGCTTGA CTTATCAGCA CTATTATACT CATTCCCGGA GGAGGACAGG 
CAAGAAGAAC TGAAGCAGGT GGAATATGCC GTTCTACGGA ACCTCACTGT CCTGCGTAAG 
ATCTACAAAT TGTACAGCCA GCTGGGAAGT GATGGCTCCC CTGACAATAC CCTTCTCATG 
ACAAAGCTGC AGTTCTGGAG GTTTCTGAAG GATTGCAAGT TTCATCACTA CAGTATCACA 
CTTTCGGAAA TGGACCGTAT CCTAACCGAG AATACTGATG CTGAAGAGGT TCATTCGCCC 
CATTCCACAA TGCTTCTTCG AACCTTTTTG ACTAATATTA TCTACTTGGC ATACCACATC 
TGTCAAACAG AAAACCAAGA GAAGAACATG TCCCTTGCTG ACTGCTTTAC TAAAGTCATG 
GTGCAGAATA TACTTCCCCA TGCAACTAAT ATCAAAGGGT TTCTATTTCC TGATGCTCAG 
AAGGCTTCCT ACGCCATTTC TTATATTGAA AAGTCTTGGC ATATCTACAG AATCTACTGC 
ATGCCAAACA CTTTCCACCC CTATGAACCG ACCATGAAAA TGAGGCACTT TCTGTTGCTA 
ATGAATGATT TAAAAATCAC AGGACTGACA GCTACAAATA TTGTTGACAT TTTGGCAGCA 
GATGACCCAT GTGTGCGTAT TGACCAGGAA GTGAACCTGG AATTGGAGCT AACGTTTCTC 
GAGTTCTTTG AGGCGCTCCT TGGCTGTGCT TCCATATGTG TTACTGAAGA GCAGATAAAG 
CAGTCAGAGA GCGAATTGTT GAAGAGAGAA GAGTCTCAGG GGAGCTCGAT TGGGGAAGTC 
ACAGCTTATC TAAAAGAAGG CCAACCAGAG CATGCTTTTC AGCCCCAAGC TGACATACCG 
CAGGACCTCT TTCTGAGAAG CTCCATGAAC CTTAAGGATA AGAGACAGAG CCCACCTATT 
CCAGTCTCTT CTATAAAGAA GCAGCAGAGA GAGGCCAGGG CAGAGAAGTG GTTCCATCAG 
ACTGACATAT TTTTCCTGAA GAAGTTCTTT CCAGCACATG AACACAAAGA ACGACTGAAG 
GAAGAAATTC CCAAAATCAG GGAGAAGCAG GCTGAAGAAG CCAGACGACA GCAGATTAGA 
GAAGAGGAAG AAGCAAGGCT TAAAGCAGAA AGGGAGGCAG AAGAGGCCAA AAGGGTTGAA 
CAGGAGTTGT TGGAAAAGGC CATGGCTGCA ATGGAAGCAG AACAGGCAGC AAAGGCACAA 
GAAGAAGAAC AGAAAAGTCC TCAGCCTCCA GTCACTCCTC ATGATGACGC TATCATTATC 
CCACCACCAT CTGGCACAAA GACTGCAACT GCAAGCAAAA AGAAGAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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rsph10b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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