rnf20 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following rnf20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rnf20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa04115 XM_012953362.2
Latest version!
Xenopus tropicalis ring finger protein 20 (rnf20), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa04115 XM_004910890.3
Latest version!
Xenopus tropicalis ring finger protein 20 (rnf20), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa04115 XM_002941865.4
Latest version!
Xenopus tropicalis ring finger protein 20 (rnf20), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa04116 XM_004910891.3
Latest version!
Xenopus tropicalis ring finger protein 20 (rnf20), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa45797 XM_018095910.1
Latest version!
Xenopus tropicalis ring finger protein 20 (rnf20), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OXa62100 XM_012953362.3
Latest version!
Xenopus tropicalis ring finger protein 20 (rnf20), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa62100 XM_004910890.4
Latest version!
Xenopus tropicalis ring finger protein 20 (rnf20), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa62100 XM_002941865.5
Latest version!
Xenopus tropicalis ring finger protein 20 (rnf20), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa62101 XM_004910891.4
Latest version!
Xenopus tropicalis ring finger protein 20 (rnf20), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa62102 XM_018095910.2
Latest version!
Xenopus tropicalis ring finger protein 20 (rnf20), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa04115
    Clone ID Related Accession (Same CDS sequence) XM_012953362.2 , XM_002941865.4 , XM_004910890.3
    Accession Version XM_012953362.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase BRE1A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012953362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGGC CTGGAAGTAA ACGTACTGTG GGGGATGTAG GTTCTTTAGG ACCCCCTGAA 
    AAAAAGGCAG CAGTGGAAGA TTCTGGAACC ACTGTCGAGA CGATTAAACT AGGAAGTGTG
    TCTTCAACAG AGGAGCAAGA TCTGAGGACC CTTCAACTGA AAAACAAGAA GTTGGCTGGA
    ATGTTAGACC AGAGACAGGC CATAGAAGAT GAACTGCGTG ATCGTATTGA GACTTTAGAA
    AGGCGGCAAG CAACAGATGA TGCTTCTCTT CTCATCATAA ACCGATACTG GAGTCAGTTT
    GATGAAAATA TTGGCATTTT TTTGGGTCGT TATGACTTGG ATCAAGACTT GGGGGAGTTT
    CTGACAGAAC GCAAAGCATT GGTTCTGCCA GAGCCAGAGC CAGATTCTGA CAGCAATCCA
    GAACGCAAAG AAAATGAAAG GGGCGAAGGA CTATGGGAAG CTCCTCTTTC CTTTTTGGCC
    ACTCTGGCCA GCAGCAGCAG TGAAGAAATT GAATCTCAGC TACAAGAACG TGTAGAATCT
    TCACGACGTG CAGTCTCTCG TATTGTTTTG GTGTATGACC GACTGCATGA CCAACTGGAC
    CATCTTGCCA AGAAACTGAA TAGCACTGAT CCATCTCACG TGGAAGAGGC TGTCAGGGAT
    TTAAACTCCT TGCTCTCAAA TGAGAATGCA CGATTGCAGG AGATTTGTAA CTTGCTACAA
    GAGAAACAGC AGAATATGTC CCAAGAGTTC CTCCAGTTGC AGTCACGATT AGAGAGTGCT
    GAATCTCGAG TCTTAGTTCT AGATGGTCAC ATTGAAGATC TTCAGTGGGA CATTGATAAA
    ATTCGGAAAA GGGAACAGCG CTTAAACAGA CATTTATCTG AAGTTCTTGA GAGGGTGAAC
    TCGAAAGGTT ATAAGGTTTA TGGAGCAGGA AGCAGTCTCT ATGGGGGAAC AATCACTATA
    AATTCTCGCA AGTTTGAAGA AATGACTTCT GAAGTTGATT TGAATAAAGA GCTGGCTGTA
    AATCGACTGC AAGAGCTTGA AAAACTGAGG CAGGATTTAC AAGAAGTGAC TTCAGAAAAT
    CAGGATTTAC AGGCTGAGTT AGCCAGTGCA GTTGAAGAGA ATGTGCGGTT GTCTCCAGAG
    TATCGCTGCA TGCAGTCTCA ATTCTCTGTA TTGTACAATG AATCACTGCA GCTAAAAACT
    CAGCTAGACG AGGCTCGTTC ACTTCTACAT GGAACACGGT CCAATCATCA GCGTCAGCTA
    GAGCTTATTG AGCGTGATGA AATTTCCCTC CAGAAAAAGG TGCGCACTGA GGTTATTCAG
    TTAGAGGACA CCCTTGCTCA GGTGCGCAAG GAATATGAAA TGCTGAGGAT AGAGTTTGAG
    CAGACCCTGG CAGCCAATGA GCAAGCAGGG CCTATTAACC GAGAAATGAG ACATTTAATC
    AGCAGTCTAC AGAATCATAA TCACCAACTT AAAGGAGAGG TATTGCGGTA TAAAAGACGT
    CTGAGAGAGA TCCAAGGGGA TATAAGCAAA ATGCGTTCTC GCAGTAGCAG TAGTCTTTTC
    CTTCTGCCAT CACAGTCCAG CACTGAGGAC ACTCGTGAAG AGACAGCTGA AATTAAGACT
    GAACCTGAAG ATACAACCAC AAATGCTTCT GCCCTTTCTC AGCCAGAGGT AGTTCCAAAG
    AGGGAGGAGG AGGAGGTACA GCCACCACTG CAACAACCAC AGCGGGATAG AAGAGAGAGG
    GAAAGGGAGC GTGAAAGAGA TCGTGGAAGA GAGAAGGAGA GAGGGGATAC TTCAAAGGAG
    AAACCAAAGC ATGAGGCAGA CACTAAGCGC AAAGAGGCAG AGACGGTCAA ACAGATGAAG
    GCAGAGCTAA AGAGAGTCCA GGAATCTCTA CGAGACATGA AGCTGCTATT AGATATGTAT
    CGTTCGGCAC CAAAGGAACA AAGAGACAAA GTGCAGCTGA TGGCAGCGGA GAGAAAAAGT
    AAAGCAGAGT TGGAGGAGCT TCGTCTTCGG CTTAGAGAGC TGGAAGAAAG GGAACGCAGG
    GACAGCAAGA AGATGGCAGA CGAAGATGCC TTACGTAGAA TGAGAACAGC TGAGGAGCAG
    ACAGAGGTTC TTCAAAAAAG ACTATCAGTG GCTAAGCAGG AGGAAGAGGC CCTGCTTTCA
    GAAATGGATG TCACAGGACA GGCATTTGAA GACATGCAGG AGCAAAATAT TCGTCTTATG
    CAGCAATTGA GGGAGAAGGA TGATGCAAAC TTTAAACTTA TGTCTGAGAG AATCAAATCA
    AACCAAATCC ATAAGCTGCT CAAGGAAGAA AAGGAGGAGC TGGCAGACCA ACTTCTTACA
    CTGCGGACAC AGGTGGATGC ACAACTCCAG GTTCTAAGGA AGCTGGAAGA GAAGGAACAT
    TTACTTCAGA GTAGCATTAA TACTGGAGAG AAAGAACTTG CTCTCCGAAC CCAGGCTCTG
    GATCTGTGCA AACGTAAGAC AACAGAATCC TCTCAGCAAG CAGAAGAATT GAGAGCTCAG
    CTGGAGCTGT CTCAAAAGAA GCTGCAAGAT CTACGTGAGG AAATAATTGA GAACAGTTCA
    AGTCGAGAGA AGGATGCTTT TAATTATAAA AGAGCTCAGG AAGATATCTC TCGCCTTCGA
    AAGAAATTGG AATCTACAAA GAAACCTGAT CTGGTGCCAA CTTGCGATAA GATTTTGATG
    GAGGAAATCA AAGAGTATAA GGCCCGACTT ACGTGCCCAT GCTGCAACTC TCGTAAAATG
    GATGCCGTTC TAACAAAATG TTTTCATGTG TTCTGCTTCG AGTGTGTTAA GACTCGCTAT
    GATACAAGGC AGCGAAAATG TCCCAAGTGC AATGCAGCTT TTGGAGCAAA TGACTTCCAT
    CGGATTTACA TTGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012808816.1
    CDS115..3012
    Translation

    Target ORF information:

    RefSeq Version XM_012953362.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger protein 20 (rnf20), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012953362.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGGC CTGGAAGTAA ACGTACTGTG GGGGATGTAG GTTCTTTAGG ACCCCCTGAA 
    AAAAAGGCAG CAGTGGAAGA TTCTGGAACC ACTGTCGAGA CGATTAAACT AGGAAGTGTG
    TCTTCAACAG AGGAGCAAGA TCTGAGGACC CTTCAACTGA AAAACAAGAA GTTGGCTGGA
    ATGTTAGACC AGAGACAGGC CATAGAAGAT GAACTGCGTG ATCGTATTGA GACTTTAGAA
    AGGCGGCAAG CAACAGATGA TGCTTCTCTT CTCATCATAA ACCGATACTG GAGTCAGTTT
    GATGAAAATA TTGGCATTTT TTTGGGTCGT TATGACTTGG ATCAAGACTT GGGGGAGTTT
    CTGACAGAAC GCAAAGCATT GGTTCTGCCA GAGCCAGAGC CAGATTCTGA CAGCAATCCA
    GAACGCAAAG AAAATGAAAG GGGCGAAGGA CTATGGGAAG CTCCTCTTTC CTTTTTGGCC
    ACTCTGGCCA GCAGCAGCAG TGAAGAAATT GAATCTCAGC TACAAGAACG TGTAGAATCT
    TCACGACGTG CAGTCTCTCG TATTGTTTTG GTGTATGACC GACTGCATGA CCAACTGGAC
    CATCTTGCCA AGAAACTGAA TAGCACTGAT CCATCTCACG TGGAAGAGGC TGTCAGGGAT
    TTAAACTCCT TGCTCTCAAA TGAGAATGCA CGATTGCAGG AGATTTGTAA CTTGCTACAA
    GAGAAACAGC AGAATATGTC CCAAGAGTTC CTCCAGTTGC AGTCACGATT AGAGAGTGCT
    GAATCTCGAG TCTTAGTTCT AGATGGTCAC ATTGAAGATC TTCAGTGGGA CATTGATAAA
    ATTCGGAAAA GGGAACAGCG CTTAAACAGA CATTTATCTG AAGTTCTTGA GAGGGTGAAC
    TCGAAAGGTT ATAAGGTTTA TGGAGCAGGA AGCAGTCTCT ATGGGGGAAC AATCACTATA
    AATTCTCGCA AGTTTGAAGA AATGACTTCT GAAGTTGATT TGAATAAAGA GCTGGCTGTA
    AATCGACTGC AAGAGCTTGA AAAACTGAGG CAGGATTTAC AAGAAGTGAC TTCAGAAAAT
    CAGGATTTAC AGGCTGAGTT AGCCAGTGCA GTTGAAGAGA ATGTGCGGTT GTCTCCAGAG
    TATCGCTGCA TGCAGTCTCA ATTCTCTGTA TTGTACAATG AATCACTGCA GCTAAAAACT
    CAGCTAGACG AGGCTCGTTC ACTTCTACAT GGAACACGGT CCAATCATCA GCGTCAGCTA
    GAGCTTATTG AGCGTGATGA AATTTCCCTC CAGAAAAAGG TGCGCACTGA GGTTATTCAG
    TTAGAGGACA CCCTTGCTCA GGTGCGCAAG GAATATGAAA TGCTGAGGAT AGAGTTTGAG
    CAGACCCTGG CAGCCAATGA GCAAGCAGGG CCTATTAACC GAGAAATGAG ACATTTAATC
    AGCAGTCTAC AGAATCATAA TCACCAACTT AAAGGAGAGG TATTGCGGTA TAAAAGACGT
    CTGAGAGAGA TCCAAGGGGA TATAAGCAAA ATGCGTTCTC GCAGTAGCAG TAGTCTTTTC
    CTTCTGCCAT CACAGTCCAG CACTGAGGAC ACTCGTGAAG AGACAGCTGA AATTAAGACT
    GAACCTGAAG ATACAACCAC AAATGCTTCT GCCCTTTCTC AGCCAGAGGT AGTTCCAAAG
    AGGGAGGAGG AGGAGGTACA GCCACCACTG CAACAACCAC AGCGGGATAG AAGAGAGAGG
    GAAAGGGAGC GTGAAAGAGA TCGTGGAAGA GAGAAGGAGA GAGGGGATAC TTCAAAGGAG
    AAACCAAAGC ATGAGGCAGA CACTAAGCGC AAAGAGGCAG AGACGGTCAA ACAGATGAAG
    GCAGAGCTAA AGAGAGTCCA GGAATCTCTA CGAGACATGA AGCTGCTATT AGATATGTAT
    CGTTCGGCAC CAAAGGAACA AAGAGACAAA GTGCAGCTGA TGGCAGCGGA GAGAAAAAGT
    AAAGCAGAGT TGGAGGAGCT TCGTCTTCGG CTTAGAGAGC TGGAAGAAAG GGAACGCAGG
    GACAGCAAGA AGATGGCAGA CGAAGATGCC TTACGTAGAA TGAGAACAGC TGAGGAGCAG
    ACAGAGGTTC TTCAAAAAAG ACTATCAGTG GCTAAGCAGG AGGAAGAGGC CCTGCTTTCA
    GAAATGGATG TCACAGGACA GGCATTTGAA GACATGCAGG AGCAAAATAT TCGTCTTATG
    CAGCAATTGA GGGAGAAGGA TGATGCAAAC TTTAAACTTA TGTCTGAGAG AATCAAATCA
    AACCAAATCC ATAAGCTGCT CAAGGAAGAA AAGGAGGAGC TGGCAGACCA ACTTCTTACA
    CTGCGGACAC AGGTGGATGC ACAACTCCAG GTTCTAAGGA AGCTGGAAGA GAAGGAACAT
    TTACTTCAGA GTAGCATTAA TACTGGAGAG AAAGAACTTG CTCTCCGAAC CCAGGCTCTG
    GATCTGTGCA AACGTAAGAC AACAGAATCC TCTCAGCAAG CAGAAGAATT GAGAGCTCAG
    CTGGAGCTGT CTCAAAAGAA GCTGCAAGAT CTACGTGAGG AAATAATTGA GAACAGTTCA
    AGTCGAGAGA AGGATGCTTT TAATTATAAA AGAGCTCAGG AAGATATCTC TCGCCTTCGA
    AAGAAATTGG AATCTACAAA GAAACCTGAT CTGGTGCCAA CTTGCGATAA GATTTTGATG
    GAGGAAATCA AAGAGTATAA GGCCCGACTT ACGTGCCCAT GCTGCAACTC TCGTAAAATG
    GATGCCGTTC TAACAAAATG TTTTCATGTG TTCTGCTTCG AGTGTGTTAA GACTCGCTAT
    GATACAAGGC AGCGAAAATG TCCCAAGTGC AATGCAGCTT TTGGAGCAAA TGACTTCCAT
    CGGATTTACA TTGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04115
    Clone ID Related Accession (Same CDS sequence) XM_012953362.2 , XM_002941865.4 , XM_004910890.3
    Accession Version XM_002941865.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase BRE1A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002941865.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGGC CTGGAAGTAA ACGTACTGTG GGGGATGTAG GTTCTTTAGG ACCCCCTGAA 
    AAAAAGGCAG CAGTGGAAGA TTCTGGAACC ACTGTCGAGA CGATTAAACT AGGAAGTGTG
    TCTTCAACAG AGGAGCAAGA TCTGAGGACC CTTCAACTGA AAAACAAGAA GTTGGCTGGA
    ATGTTAGACC AGAGACAGGC CATAGAAGAT GAACTGCGTG ATCGTATTGA GACTTTAGAA
    AGGCGGCAAG CAACAGATGA TGCTTCTCTT CTCATCATAA ACCGATACTG GAGTCAGTTT
    GATGAAAATA TTGGCATTTT TTTGGGTCGT TATGACTTGG ATCAAGACTT GGGGGAGTTT
    CTGACAGAAC GCAAAGCATT GGTTCTGCCA GAGCCAGAGC CAGATTCTGA CAGCAATCCA
    GAACGCAAAG AAAATGAAAG GGGCGAAGGA CTATGGGAAG CTCCTCTTTC CTTTTTGGCC
    ACTCTGGCCA GCAGCAGCAG TGAAGAAATT GAATCTCAGC TACAAGAACG TGTAGAATCT
    TCACGACGTG CAGTCTCTCG TATTGTTTTG GTGTATGACC GACTGCATGA CCAACTGGAC
    CATCTTGCCA AGAAACTGAA TAGCACTGAT CCATCTCACG TGGAAGAGGC TGTCAGGGAT
    TTAAACTCCT TGCTCTCAAA TGAGAATGCA CGATTGCAGG AGATTTGTAA CTTGCTACAA
    GAGAAACAGC AGAATATGTC CCAAGAGTTC CTCCAGTTGC AGTCACGATT AGAGAGTGCT
    GAATCTCGAG TCTTAGTTCT AGATGGTCAC ATTGAAGATC TTCAGTGGGA CATTGATAAA
    ATTCGGAAAA GGGAACAGCG CTTAAACAGA CATTTATCTG AAGTTCTTGA GAGGGTGAAC
    TCGAAAGGTT ATAAGGTTTA TGGAGCAGGA AGCAGTCTCT ATGGGGGAAC AATCACTATA
    AATTCTCGCA AGTTTGAAGA AATGACTTCT GAAGTTGATT TGAATAAAGA GCTGGCTGTA
    AATCGACTGC AAGAGCTTGA AAAACTGAGG CAGGATTTAC AAGAAGTGAC TTCAGAAAAT
    CAGGATTTAC AGGCTGAGTT AGCCAGTGCA GTTGAAGAGA ATGTGCGGTT GTCTCCAGAG
    TATCGCTGCA TGCAGTCTCA ATTCTCTGTA TTGTACAATG AATCACTGCA GCTAAAAACT
    CAGCTAGACG AGGCTCGTTC ACTTCTACAT GGAACACGGT CCAATCATCA GCGTCAGCTA
    GAGCTTATTG AGCGTGATGA AATTTCCCTC CAGAAAAAGG TGCGCACTGA GGTTATTCAG
    TTAGAGGACA CCCTTGCTCA GGTGCGCAAG GAATATGAAA TGCTGAGGAT AGAGTTTGAG
    CAGACCCTGG CAGCCAATGA GCAAGCAGGG CCTATTAACC GAGAAATGAG ACATTTAATC
    AGCAGTCTAC AGAATCATAA TCACCAACTT AAAGGAGAGG TATTGCGGTA TAAAAGACGT
    CTGAGAGAGA TCCAAGGGGA TATAAGCAAA ATGCGTTCTC GCAGTAGCAG TAGTCTTTTC
    CTTCTGCCAT CACAGTCCAG CACTGAGGAC ACTCGTGAAG AGACAGCTGA AATTAAGACT
    GAACCTGAAG ATACAACCAC AAATGCTTCT GCCCTTTCTC AGCCAGAGGT AGTTCCAAAG
    AGGGAGGAGG AGGAGGTACA GCCACCACTG CAACAACCAC AGCGGGATAG AAGAGAGAGG
    GAAAGGGAGC GTGAAAGAGA TCGTGGAAGA GAGAAGGAGA GAGGGGATAC TTCAAAGGAG
    AAACCAAAGC ATGAGGCAGA CACTAAGCGC AAAGAGGCAG AGACGGTCAA ACAGATGAAG
    GCAGAGCTAA AGAGAGTCCA GGAATCTCTA CGAGACATGA AGCTGCTATT AGATATGTAT
    CGTTCGGCAC CAAAGGAACA AAGAGACAAA GTGCAGCTGA TGGCAGCGGA GAGAAAAAGT
    AAAGCAGAGT TGGAGGAGCT TCGTCTTCGG CTTAGAGAGC TGGAAGAAAG GGAACGCAGG
    GACAGCAAGA AGATGGCAGA CGAAGATGCC TTACGTAGAA TGAGAACAGC TGAGGAGCAG
    ACAGAGGTTC TTCAAAAAAG ACTATCAGTG GCTAAGCAGG AGGAAGAGGC CCTGCTTTCA
    GAAATGGATG TCACAGGACA GGCATTTGAA GACATGCAGG AGCAAAATAT TCGTCTTATG
    CAGCAATTGA GGGAGAAGGA TGATGCAAAC TTTAAACTTA TGTCTGAGAG AATCAAATCA
    AACCAAATCC ATAAGCTGCT CAAGGAAGAA AAGGAGGAGC TGGCAGACCA ACTTCTTACA
    CTGCGGACAC AGGTGGATGC ACAACTCCAG GTTCTAAGGA AGCTGGAAGA GAAGGAACAT
    TTACTTCAGA GTAGCATTAA TACTGGAGAG AAAGAACTTG CTCTCCGAAC CCAGGCTCTG
    GATCTGTGCA AACGTAAGAC AACAGAATCC TCTCAGCAAG CAGAAGAATT GAGAGCTCAG
    CTGGAGCTGT CTCAAAAGAA GCTGCAAGAT CTACGTGAGG AAATAATTGA GAACAGTTCA
    AGTCGAGAGA AGGATGCTTT TAATTATAAA AGAGCTCAGG AAGATATCTC TCGCCTTCGA
    AAGAAATTGG AATCTACAAA GAAACCTGAT CTGGTGCCAA CTTGCGATAA GATTTTGATG
    GAGGAAATCA AAGAGTATAA GGCCCGACTT ACGTGCCCAT GCTGCAACTC TCGTAAAATG
    GATGCCGTTC TAACAAAATG TTTTCATGTG TTCTGCTTCG AGTGTGTTAA GACTCGCTAT
    GATACAAGGC AGCGAAAATG TCCCAAGTGC AATGCAGCTT TTGGAGCAAA TGACTTCCAT
    CGGATTTACA TTGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002941911.1
    CDS164..3061
    Translation

    Target ORF information:

    RefSeq Version XM_002941865.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger protein 20 (rnf20), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002941865.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGGC CTGGAAGTAA ACGTACTGTG GGGGATGTAG GTTCTTTAGG ACCCCCTGAA 
    AAAAAGGCAG CAGTGGAAGA TTCTGGAACC ACTGTCGAGA CGATTAAACT AGGAAGTGTG
    TCTTCAACAG AGGAGCAAGA TCTGAGGACC CTTCAACTGA AAAACAAGAA GTTGGCTGGA
    ATGTTAGACC AGAGACAGGC CATAGAAGAT GAACTGCGTG ATCGTATTGA GACTTTAGAA
    AGGCGGCAAG CAACAGATGA TGCTTCTCTT CTCATCATAA ACCGATACTG GAGTCAGTTT
    GATGAAAATA TTGGCATTTT TTTGGGTCGT TATGACTTGG ATCAAGACTT GGGGGAGTTT
    CTGACAGAAC GCAAAGCATT GGTTCTGCCA GAGCCAGAGC CAGATTCTGA CAGCAATCCA
    GAACGCAAAG AAAATGAAAG GGGCGAAGGA CTATGGGAAG CTCCTCTTTC CTTTTTGGCC
    ACTCTGGCCA GCAGCAGCAG TGAAGAAATT GAATCTCAGC TACAAGAACG TGTAGAATCT
    TCACGACGTG CAGTCTCTCG TATTGTTTTG GTGTATGACC GACTGCATGA CCAACTGGAC
    CATCTTGCCA AGAAACTGAA TAGCACTGAT CCATCTCACG TGGAAGAGGC TGTCAGGGAT
    TTAAACTCCT TGCTCTCAAA TGAGAATGCA CGATTGCAGG AGATTTGTAA CTTGCTACAA
    GAGAAACAGC AGAATATGTC CCAAGAGTTC CTCCAGTTGC AGTCACGATT AGAGAGTGCT
    GAATCTCGAG TCTTAGTTCT AGATGGTCAC ATTGAAGATC TTCAGTGGGA CATTGATAAA
    ATTCGGAAAA GGGAACAGCG CTTAAACAGA CATTTATCTG AAGTTCTTGA GAGGGTGAAC
    TCGAAAGGTT ATAAGGTTTA TGGAGCAGGA AGCAGTCTCT ATGGGGGAAC AATCACTATA
    AATTCTCGCA AGTTTGAAGA AATGACTTCT GAAGTTGATT TGAATAAAGA GCTGGCTGTA
    AATCGACTGC AAGAGCTTGA AAAACTGAGG CAGGATTTAC AAGAAGTGAC TTCAGAAAAT
    CAGGATTTAC AGGCTGAGTT AGCCAGTGCA GTTGAAGAGA ATGTGCGGTT GTCTCCAGAG
    TATCGCTGCA TGCAGTCTCA ATTCTCTGTA TTGTACAATG AATCACTGCA GCTAAAAACT
    CAGCTAGACG AGGCTCGTTC ACTTCTACAT GGAACACGGT CCAATCATCA GCGTCAGCTA
    GAGCTTATTG AGCGTGATGA AATTTCCCTC CAGAAAAAGG TGCGCACTGA GGTTATTCAG
    TTAGAGGACA CCCTTGCTCA GGTGCGCAAG GAATATGAAA TGCTGAGGAT AGAGTTTGAG
    CAGACCCTGG CAGCCAATGA GCAAGCAGGG CCTATTAACC GAGAAATGAG ACATTTAATC
    AGCAGTCTAC AGAATCATAA TCACCAACTT AAAGGAGAGG TATTGCGGTA TAAAAGACGT
    CTGAGAGAGA TCCAAGGGGA TATAAGCAAA ATGCGTTCTC GCAGTAGCAG TAGTCTTTTC
    CTTCTGCCAT CACAGTCCAG CACTGAGGAC ACTCGTGAAG AGACAGCTGA AATTAAGACT
    GAACCTGAAG ATACAACCAC AAATGCTTCT GCCCTTTCTC AGCCAGAGGT AGTTCCAAAG
    AGGGAGGAGG AGGAGGTACA GCCACCACTG CAACAACCAC AGCGGGATAG AAGAGAGAGG
    GAAAGGGAGC GTGAAAGAGA TCGTGGAAGA GAGAAGGAGA GAGGGGATAC TTCAAAGGAG
    AAACCAAAGC ATGAGGCAGA CACTAAGCGC AAAGAGGCAG AGACGGTCAA ACAGATGAAG
    GCAGAGCTAA AGAGAGTCCA GGAATCTCTA CGAGACATGA AGCTGCTATT AGATATGTAT
    CGTTCGGCAC CAAAGGAACA AAGAGACAAA GTGCAGCTGA TGGCAGCGGA GAGAAAAAGT
    AAAGCAGAGT TGGAGGAGCT TCGTCTTCGG CTTAGAGAGC TGGAAGAAAG GGAACGCAGG
    GACAGCAAGA AGATGGCAGA CGAAGATGCC TTACGTAGAA TGAGAACAGC TGAGGAGCAG
    ACAGAGGTTC TTCAAAAAAG ACTATCAGTG GCTAAGCAGG AGGAAGAGGC CCTGCTTTCA
    GAAATGGATG TCACAGGACA GGCATTTGAA GACATGCAGG AGCAAAATAT TCGTCTTATG
    CAGCAATTGA GGGAGAAGGA TGATGCAAAC TTTAAACTTA TGTCTGAGAG AATCAAATCA
    AACCAAATCC ATAAGCTGCT CAAGGAAGAA AAGGAGGAGC TGGCAGACCA ACTTCTTACA
    CTGCGGACAC AGGTGGATGC ACAACTCCAG GTTCTAAGGA AGCTGGAAGA GAAGGAACAT
    TTACTTCAGA GTAGCATTAA TACTGGAGAG AAAGAACTTG CTCTCCGAAC CCAGGCTCTG
    GATCTGTGCA AACGTAAGAC AACAGAATCC TCTCAGCAAG CAGAAGAATT GAGAGCTCAG
    CTGGAGCTGT CTCAAAAGAA GCTGCAAGAT CTACGTGAGG AAATAATTGA GAACAGTTCA
    AGTCGAGAGA AGGATGCTTT TAATTATAAA AGAGCTCAGG AAGATATCTC TCGCCTTCGA
    AAGAAATTGG AATCTACAAA GAAACCTGAT CTGGTGCCAA CTTGCGATAA GATTTTGATG
    GAGGAAATCA AAGAGTATAA GGCCCGACTT ACGTGCCCAT GCTGCAACTC TCGTAAAATG
    GATGCCGTTC TAACAAAATG TTTTCATGTG TTCTGCTTCG AGTGTGTTAA GACTCGCTAT
    GATACAAGGC AGCGAAAATG TCCCAAGTGC AATGCAGCTT TTGGAGCAAA TGACTTCCAT
    CGGATTTACA TTGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04115
    Clone ID Related Accession (Same CDS sequence) XM_012953362.2 , XM_002941865.4 , XM_004910890.3
    Accession Version XM_004910890.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase BRE1A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910890.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGGC CTGGAAGTAA ACGTACTGTG GGGGATGTAG GTTCTTTAGG ACCCCCTGAA 
    AAAAAGGCAG CAGTGGAAGA TTCTGGAACC ACTGTCGAGA CGATTAAACT AGGAAGTGTG
    TCTTCAACAG AGGAGCAAGA TCTGAGGACC CTTCAACTGA AAAACAAGAA GTTGGCTGGA
    ATGTTAGACC AGAGACAGGC CATAGAAGAT GAACTGCGTG ATCGTATTGA GACTTTAGAA
    AGGCGGCAAG CAACAGATGA TGCTTCTCTT CTCATCATAA ACCGATACTG GAGTCAGTTT
    GATGAAAATA TTGGCATTTT TTTGGGTCGT TATGACTTGG ATCAAGACTT GGGGGAGTTT
    CTGACAGAAC GCAAAGCATT GGTTCTGCCA GAGCCAGAGC CAGATTCTGA CAGCAATCCA
    GAACGCAAAG AAAATGAAAG GGGCGAAGGA CTATGGGAAG CTCCTCTTTC CTTTTTGGCC
    ACTCTGGCCA GCAGCAGCAG TGAAGAAATT GAATCTCAGC TACAAGAACG TGTAGAATCT
    TCACGACGTG CAGTCTCTCG TATTGTTTTG GTGTATGACC GACTGCATGA CCAACTGGAC
    CATCTTGCCA AGAAACTGAA TAGCACTGAT CCATCTCACG TGGAAGAGGC TGTCAGGGAT
    TTAAACTCCT TGCTCTCAAA TGAGAATGCA CGATTGCAGG AGATTTGTAA CTTGCTACAA
    GAGAAACAGC AGAATATGTC CCAAGAGTTC CTCCAGTTGC AGTCACGATT AGAGAGTGCT
    GAATCTCGAG TCTTAGTTCT AGATGGTCAC ATTGAAGATC TTCAGTGGGA CATTGATAAA
    ATTCGGAAAA GGGAACAGCG CTTAAACAGA CATTTATCTG AAGTTCTTGA GAGGGTGAAC
    TCGAAAGGTT ATAAGGTTTA TGGAGCAGGA AGCAGTCTCT ATGGGGGAAC AATCACTATA
    AATTCTCGCA AGTTTGAAGA AATGACTTCT GAAGTTGATT TGAATAAAGA GCTGGCTGTA
    AATCGACTGC AAGAGCTTGA AAAACTGAGG CAGGATTTAC AAGAAGTGAC TTCAGAAAAT
    CAGGATTTAC AGGCTGAGTT AGCCAGTGCA GTTGAAGAGA ATGTGCGGTT GTCTCCAGAG
    TATCGCTGCA TGCAGTCTCA ATTCTCTGTA TTGTACAATG AATCACTGCA GCTAAAAACT
    CAGCTAGACG AGGCTCGTTC ACTTCTACAT GGAACACGGT CCAATCATCA GCGTCAGCTA
    GAGCTTATTG AGCGTGATGA AATTTCCCTC CAGAAAAAGG TGCGCACTGA GGTTATTCAG
    TTAGAGGACA CCCTTGCTCA GGTGCGCAAG GAATATGAAA TGCTGAGGAT AGAGTTTGAG
    CAGACCCTGG CAGCCAATGA GCAAGCAGGG CCTATTAACC GAGAAATGAG ACATTTAATC
    AGCAGTCTAC AGAATCATAA TCACCAACTT AAAGGAGAGG TATTGCGGTA TAAAAGACGT
    CTGAGAGAGA TCCAAGGGGA TATAAGCAAA ATGCGTTCTC GCAGTAGCAG TAGTCTTTTC
    CTTCTGCCAT CACAGTCCAG CACTGAGGAC ACTCGTGAAG AGACAGCTGA AATTAAGACT
    GAACCTGAAG ATACAACCAC AAATGCTTCT GCCCTTTCTC AGCCAGAGGT AGTTCCAAAG
    AGGGAGGAGG AGGAGGTACA GCCACCACTG CAACAACCAC AGCGGGATAG AAGAGAGAGG
    GAAAGGGAGC GTGAAAGAGA TCGTGGAAGA GAGAAGGAGA GAGGGGATAC TTCAAAGGAG
    AAACCAAAGC ATGAGGCAGA CACTAAGCGC AAAGAGGCAG AGACGGTCAA ACAGATGAAG
    GCAGAGCTAA AGAGAGTCCA GGAATCTCTA CGAGACATGA AGCTGCTATT AGATATGTAT
    CGTTCGGCAC CAAAGGAACA AAGAGACAAA GTGCAGCTGA TGGCAGCGGA GAGAAAAAGT
    AAAGCAGAGT TGGAGGAGCT TCGTCTTCGG CTTAGAGAGC TGGAAGAAAG GGAACGCAGG
    GACAGCAAGA AGATGGCAGA CGAAGATGCC TTACGTAGAA TGAGAACAGC TGAGGAGCAG
    ACAGAGGTTC TTCAAAAAAG ACTATCAGTG GCTAAGCAGG AGGAAGAGGC CCTGCTTTCA
    GAAATGGATG TCACAGGACA GGCATTTGAA GACATGCAGG AGCAAAATAT TCGTCTTATG
    CAGCAATTGA GGGAGAAGGA TGATGCAAAC TTTAAACTTA TGTCTGAGAG AATCAAATCA
    AACCAAATCC ATAAGCTGCT CAAGGAAGAA AAGGAGGAGC TGGCAGACCA ACTTCTTACA
    CTGCGGACAC AGGTGGATGC ACAACTCCAG GTTCTAAGGA AGCTGGAAGA GAAGGAACAT
    TTACTTCAGA GTAGCATTAA TACTGGAGAG AAAGAACTTG CTCTCCGAAC CCAGGCTCTG
    GATCTGTGCA AACGTAAGAC AACAGAATCC TCTCAGCAAG CAGAAGAATT GAGAGCTCAG
    CTGGAGCTGT CTCAAAAGAA GCTGCAAGAT CTACGTGAGG AAATAATTGA GAACAGTTCA
    AGTCGAGAGA AGGATGCTTT TAATTATAAA AGAGCTCAGG AAGATATCTC TCGCCTTCGA
    AAGAAATTGG AATCTACAAA GAAACCTGAT CTGGTGCCAA CTTGCGATAA GATTTTGATG
    GAGGAAATCA AAGAGTATAA GGCCCGACTT ACGTGCCCAT GCTGCAACTC TCGTAAAATG
    GATGCCGTTC TAACAAAATG TTTTCATGTG TTCTGCTTCG AGTGTGTTAA GACTCGCTAT
    GATACAAGGC AGCGAAAATG TCCCAAGTGC AATGCAGCTT TTGGAGCAAA TGACTTCCAT
    CGGATTTACA TTGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910947.1
    CDS125..3022
    Translation

    Target ORF information:

    RefSeq Version XM_004910890.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger protein 20 (rnf20), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910890.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGGC CTGGAAGTAA ACGTACTGTG GGGGATGTAG GTTCTTTAGG ACCCCCTGAA 
    AAAAAGGCAG CAGTGGAAGA TTCTGGAACC ACTGTCGAGA CGATTAAACT AGGAAGTGTG
    TCTTCAACAG AGGAGCAAGA TCTGAGGACC CTTCAACTGA AAAACAAGAA GTTGGCTGGA
    ATGTTAGACC AGAGACAGGC CATAGAAGAT GAACTGCGTG ATCGTATTGA GACTTTAGAA
    AGGCGGCAAG CAACAGATGA TGCTTCTCTT CTCATCATAA ACCGATACTG GAGTCAGTTT
    GATGAAAATA TTGGCATTTT TTTGGGTCGT TATGACTTGG ATCAAGACTT GGGGGAGTTT
    CTGACAGAAC GCAAAGCATT GGTTCTGCCA GAGCCAGAGC CAGATTCTGA CAGCAATCCA
    GAACGCAAAG AAAATGAAAG GGGCGAAGGA CTATGGGAAG CTCCTCTTTC CTTTTTGGCC
    ACTCTGGCCA GCAGCAGCAG TGAAGAAATT GAATCTCAGC TACAAGAACG TGTAGAATCT
    TCACGACGTG CAGTCTCTCG TATTGTTTTG GTGTATGACC GACTGCATGA CCAACTGGAC
    CATCTTGCCA AGAAACTGAA TAGCACTGAT CCATCTCACG TGGAAGAGGC TGTCAGGGAT
    TTAAACTCCT TGCTCTCAAA TGAGAATGCA CGATTGCAGG AGATTTGTAA CTTGCTACAA
    GAGAAACAGC AGAATATGTC CCAAGAGTTC CTCCAGTTGC AGTCACGATT AGAGAGTGCT
    GAATCTCGAG TCTTAGTTCT AGATGGTCAC ATTGAAGATC TTCAGTGGGA CATTGATAAA
    ATTCGGAAAA GGGAACAGCG CTTAAACAGA CATTTATCTG AAGTTCTTGA GAGGGTGAAC
    TCGAAAGGTT ATAAGGTTTA TGGAGCAGGA AGCAGTCTCT ATGGGGGAAC AATCACTATA
    AATTCTCGCA AGTTTGAAGA AATGACTTCT GAAGTTGATT TGAATAAAGA GCTGGCTGTA
    AATCGACTGC AAGAGCTTGA AAAACTGAGG CAGGATTTAC AAGAAGTGAC TTCAGAAAAT
    CAGGATTTAC AGGCTGAGTT AGCCAGTGCA GTTGAAGAGA ATGTGCGGTT GTCTCCAGAG
    TATCGCTGCA TGCAGTCTCA ATTCTCTGTA TTGTACAATG AATCACTGCA GCTAAAAACT
    CAGCTAGACG AGGCTCGTTC ACTTCTACAT GGAACACGGT CCAATCATCA GCGTCAGCTA
    GAGCTTATTG AGCGTGATGA AATTTCCCTC CAGAAAAAGG TGCGCACTGA GGTTATTCAG
    TTAGAGGACA CCCTTGCTCA GGTGCGCAAG GAATATGAAA TGCTGAGGAT AGAGTTTGAG
    CAGACCCTGG CAGCCAATGA GCAAGCAGGG CCTATTAACC GAGAAATGAG ACATTTAATC
    AGCAGTCTAC AGAATCATAA TCACCAACTT AAAGGAGAGG TATTGCGGTA TAAAAGACGT
    CTGAGAGAGA TCCAAGGGGA TATAAGCAAA ATGCGTTCTC GCAGTAGCAG TAGTCTTTTC
    CTTCTGCCAT CACAGTCCAG CACTGAGGAC ACTCGTGAAG AGACAGCTGA AATTAAGACT
    GAACCTGAAG ATACAACCAC AAATGCTTCT GCCCTTTCTC AGCCAGAGGT AGTTCCAAAG
    AGGGAGGAGG AGGAGGTACA GCCACCACTG CAACAACCAC AGCGGGATAG AAGAGAGAGG
    GAAAGGGAGC GTGAAAGAGA TCGTGGAAGA GAGAAGGAGA GAGGGGATAC TTCAAAGGAG
    AAACCAAAGC ATGAGGCAGA CACTAAGCGC AAAGAGGCAG AGACGGTCAA ACAGATGAAG
    GCAGAGCTAA AGAGAGTCCA GGAATCTCTA CGAGACATGA AGCTGCTATT AGATATGTAT
    CGTTCGGCAC CAAAGGAACA AAGAGACAAA GTGCAGCTGA TGGCAGCGGA GAGAAAAAGT
    AAAGCAGAGT TGGAGGAGCT TCGTCTTCGG CTTAGAGAGC TGGAAGAAAG GGAACGCAGG
    GACAGCAAGA AGATGGCAGA CGAAGATGCC TTACGTAGAA TGAGAACAGC TGAGGAGCAG
    ACAGAGGTTC TTCAAAAAAG ACTATCAGTG GCTAAGCAGG AGGAAGAGGC CCTGCTTTCA
    GAAATGGATG TCACAGGACA GGCATTTGAA GACATGCAGG AGCAAAATAT TCGTCTTATG
    CAGCAATTGA GGGAGAAGGA TGATGCAAAC TTTAAACTTA TGTCTGAGAG AATCAAATCA
    AACCAAATCC ATAAGCTGCT CAAGGAAGAA AAGGAGGAGC TGGCAGACCA ACTTCTTACA
    CTGCGGACAC AGGTGGATGC ACAACTCCAG GTTCTAAGGA AGCTGGAAGA GAAGGAACAT
    TTACTTCAGA GTAGCATTAA TACTGGAGAG AAAGAACTTG CTCTCCGAAC CCAGGCTCTG
    GATCTGTGCA AACGTAAGAC AACAGAATCC TCTCAGCAAG CAGAAGAATT GAGAGCTCAG
    CTGGAGCTGT CTCAAAAGAA GCTGCAAGAT CTACGTGAGG AAATAATTGA GAACAGTTCA
    AGTCGAGAGA AGGATGCTTT TAATTATAAA AGAGCTCAGG AAGATATCTC TCGCCTTCGA
    AAGAAATTGG AATCTACAAA GAAACCTGAT CTGGTGCCAA CTTGCGATAA GATTTTGATG
    GAGGAAATCA AAGAGTATAA GGCCCGACTT ACGTGCCCAT GCTGCAACTC TCGTAAAATG
    GATGCCGTTC TAACAAAATG TTTTCATGTG TTCTGCTTCG AGTGTGTTAA GACTCGCTAT
    GATACAAGGC AGCGAAAATG TCCCAAGTGC AATGCAGCTT TTGGAGCAAA TGACTTCCAT
    CGGATTTACA TTGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04116
    Clone ID Related Accession (Same CDS sequence) XM_004910891.3
    Accession Version XM_004910891.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2853bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase BRE1A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910891.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTCAGGGC CTGGAAGTAA ACGTACTGTG GGGGATGTAG GTTCTTTAGG ACCCCCTGAA 
    AAAAAGGCAG CAGTGGAAGA TTCTGGAACC ACTGTCGAGA CGATTAAACT AGGAAGTGTG
    TCTTCAACAG AGGAGCAAGA TCTGAGGACC CTTCAACTGA AAAACAAGAA GTTGGCTGGA
    ATGTTAGACC AGAGACAGGC CATAGAAGAT GAACTGCGTG ATCGTATTGA GACTTTAGAA
    AGGCGGCAAG CAACAGATGA TGCTTCTCTT CTCATCATAA ACCGATACTG GAGTCAGTTT
    GATGAAAATA TTGGCATTTT TTTGGGTCGT TATGACTTGG ATCAAGACTT GGGGGAGTTT
    CTGACAGAAC GCAAAGCATT GGTTCTGCCA GAGCCAGAGC CAGATTCTGA CAGCAATCCA
    GAACGCAAAG AAAATGAAAG GGGCGAAGGA CTATGGGAAG CTCCTCTTTC CTTTTTGGCC
    ACTCTGGCCA GCAGCAGCAG TGAAGAAATT GAATCTCAGC TACAAGAACG TGTAGAATCT
    TCACGACGTG CAGTCTCTCG TATTGTTTTG GTGTATGACC GACTGCATGA CCAACTGGAC
    CATCTTGCCA AGAAACTGAA TAGCACTGAT CCATCTCACG TGGAAGAGGC TGTCAGGGAT
    TTAAACTCCT TGCTCTCAAA TGAGAATGCA CGATTGCAGG AGATTTGTAA CTTGCTACAA
    GAGAAACAGC AGAATATGTC CCAAGAGTTC CTCCAGTTGC AGTCACGATT AGAGAGTGCT
    GAATCTCGAG TCTTAGTTCT AGATGGTCAC ATTGAAGATC TTCAGTGGGA CATTGATAAA
    ATTCGGAAAA GGGAACAGCG CTTAAACAGA CATTTATCTG AAGTTCTTGA GAGGGTGAAC
    TCGAAAGGTT ATAAGGTTTA TGGAGCAGGA AGCAGTCTCT ATGGGGGAAC AATCACTATA
    AATTCTCGCA AGTTTGAAGA AATGACTTCT GAAGTTGATT TGAATAAAGA GCTGGCTGTA
    AATCGACTGC AAGAGCTTGA AAAACTGAGG CAGGATTTAC AAGAAGTGAC TTCAGAAAAT
    CAGGATTTAC AGGCTGAGTT AGCCAGTGCA GTTGAAGAGA ATGTGCGGTT GTCTCCAGAG
    TATCGCTGCA TGCAGTCTCA ATTCTCTGTA TTGTACAATG AATCACTGCA GCTAAAAACT
    CAGCTAGACG AGGCTCGTTC ACTTCTACAT GGAACACGGT CCAATCATCA GCGTCAGCTA
    GAGCTTATTG AGCGTGATGA AATTTCCCTC CAGAAAAAGG TGCGCACTGA GGTTATTCAG
    TTAGAGGACA CCCTTGCTCA GGTGCGCAAG GAATATGAAA TGCTGAGGAT AGAGTTTGAG
    CAGACCCTGG CAGCCAATGA GCAAGCAGGG CCTATTAACC GAGAAATGAG ACATTTAATC
    AGCAGTCTAC AGAATCATAA TCACCAACTT AAAGGAGAGG TATTGCGGTA TAAAAGACGT
    CTGAGAGAGA TCCAAGGGGA TATAAGCAAA TCCAGCACTG AGGACACTCG TGAAGAGACA
    GCTGAAATTA AGACTGAACC TGAAGATACA ACCACAAATG CTTCTGCCCT TTCTCAGCCA
    GAGGTAGTTC CAAAGAGGGA GGAGGAGGAG GTACAGCCAC CACTGCAACA ACCACAGCGG
    GATAGAAGAG AGAGGGAAAG GGAGCGTGAA AGAGATCGTG GAAGAGAGAA GGAGAGAGGG
    GATACTTCAA AGGAGAAACC AAAGCATGAG GCAGACACTA AGCGCAAAGA GGCAGAGACG
    GTCAAACAGA TGAAGGCAGA GCTAAAGAGA GTCCAGGAAT CTCTACGAGA CATGAAGCTG
    CTATTAGATA TGTATCGTTC GGCACCAAAG GAACAAAGAG ACAAAGTGCA GCTGATGGCA
    GCGGAGAGAA AAAGTAAAGC AGAGTTGGAG GAGCTTCGTC TTCGGCTTAG AGAGCTGGAA
    GAAAGGGAAC GCAGGGACAG CAAGAAGATG GCAGACGAAG ATGCCTTACG TAGAATGAGA
    ACAGCTGAGG AGCAGACAGA GGTTCTTCAA AAAAGACTAT CAGTGGCTAA GCAGGAGGAA
    GAGGCCCTGC TTTCAGAAAT GGATGTCACA GGACAGGCAT TTGAAGACAT GCAGGAGCAA
    AATATTCGTC TTATGCAGCA ATTGAGGGAG AAGGATGATG CAAACTTTAA ACTTATGTCT
    GAGAGAATCA AATCAAACCA AATCCATAAG CTGCTCAAGG AAGAAAAGGA GGAGCTGGCA
    GACCAACTTC TTACACTGCG GACACAGGTG GATGCACAAC TCCAGGTTCT AAGGAAGCTG
    GAAGAGAAGG AACATTTACT TCAGAGTAGC ATTAATACTG GAGAGAAAGA ACTTGCTCTC
    CGAACCCAGG CTCTGGATCT GTGCAAACGT AAGACAACAG AATCCTCTCA GCAAGCAGAA
    GAATTGAGAG CTCAGCTGGA GCTGTCTCAA AAGAAGCTGC AAGATCTACG TGAGGAAATA
    ATTGAGAACA GTTCAAGTCG AGAGAAGGAT GCTTTTAATT ATAAAAGAGC TCAGGAAGAT
    ATCTCTCGCC TTCGAAAGAA ATTGGAATCT ACAAAGAAAC CTGATCTGGT GCCAACTTGC
    GATAAGATTT TGATGGAGGA AATCAAAGAG TATAAGGCCC GACTTACGTG CCCATGCTGC
    AACTCTCGTA AAATGGATGC CGTTCTAACA AAATGTTTTC ATGTGTTCTG CTTCGAGTGT
    GTTAAGACTC GCTATGATAC AAGGCAGCGA AAATGTCCCA AGTGCAATGC AGCTTTTGGA
    GCAAATGACT TCCATCGGAT TTACATTGGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910948.1
    CDS165..3017
    Translation

    Target ORF information:

    RefSeq Version XM_004910891.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger protein 20 (rnf20), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910891.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTCAGGGC CTGGAAGTAA ACGTACTGTG GGGGATGTAG GTTCTTTAGG ACCCCCTGAA 
    AAAAAGGCAG CAGTGGAAGA TTCTGGAACC ACTGTCGAGA CGATTAAACT AGGAAGTGTG
    TCTTCAACAG AGGAGCAAGA TCTGAGGACC CTTCAACTGA AAAACAAGAA GTTGGCTGGA
    ATGTTAGACC AGAGACAGGC CATAGAAGAT GAACTGCGTG ATCGTATTGA GACTTTAGAA
    AGGCGGCAAG CAACAGATGA TGCTTCTCTT CTCATCATAA ACCGATACTG GAGTCAGTTT
    GATGAAAATA TTGGCATTTT TTTGGGTCGT TATGACTTGG ATCAAGACTT GGGGGAGTTT
    CTGACAGAAC GCAAAGCATT GGTTCTGCCA GAGCCAGAGC CAGATTCTGA CAGCAATCCA
    GAACGCAAAG AAAATGAAAG GGGCGAAGGA CTATGGGAAG CTCCTCTTTC CTTTTTGGCC
    ACTCTGGCCA GCAGCAGCAG TGAAGAAATT GAATCTCAGC TACAAGAACG TGTAGAATCT
    TCACGACGTG CAGTCTCTCG TATTGTTTTG GTGTATGACC GACTGCATGA CCAACTGGAC
    CATCTTGCCA AGAAACTGAA TAGCACTGAT CCATCTCACG TGGAAGAGGC TGTCAGGGAT
    TTAAACTCCT TGCTCTCAAA TGAGAATGCA CGATTGCAGG AGATTTGTAA CTTGCTACAA
    GAGAAACAGC AGAATATGTC CCAAGAGTTC CTCCAGTTGC AGTCACGATT AGAGAGTGCT
    GAATCTCGAG TCTTAGTTCT AGATGGTCAC ATTGAAGATC TTCAGTGGGA CATTGATAAA
    ATTCGGAAAA GGGAACAGCG CTTAAACAGA CATTTATCTG AAGTTCTTGA GAGGGTGAAC
    TCGAAAGGTT ATAAGGTTTA TGGAGCAGGA AGCAGTCTCT ATGGGGGAAC AATCACTATA
    AATTCTCGCA AGTTTGAAGA AATGACTTCT GAAGTTGATT TGAATAAAGA GCTGGCTGTA
    AATCGACTGC AAGAGCTTGA AAAACTGAGG CAGGATTTAC AAGAAGTGAC TTCAGAAAAT
    CAGGATTTAC AGGCTGAGTT AGCCAGTGCA GTTGAAGAGA ATGTGCGGTT GTCTCCAGAG
    TATCGCTGCA TGCAGTCTCA ATTCTCTGTA TTGTACAATG AATCACTGCA GCTAAAAACT
    CAGCTAGACG AGGCTCGTTC ACTTCTACAT GGAACACGGT CCAATCATCA GCGTCAGCTA
    GAGCTTATTG AGCGTGATGA AATTTCCCTC CAGAAAAAGG TGCGCACTGA GGTTATTCAG
    TTAGAGGACA CCCTTGCTCA GGTGCGCAAG GAATATGAAA TGCTGAGGAT AGAGTTTGAG
    CAGACCCTGG CAGCCAATGA GCAAGCAGGG CCTATTAACC GAGAAATGAG ACATTTAATC
    AGCAGTCTAC AGAATCATAA TCACCAACTT AAAGGAGAGG TATTGCGGTA TAAAAGACGT
    CTGAGAGAGA TCCAAGGGGA TATAAGCAAA TCCAGCACTG AGGACACTCG TGAAGAGACA
    GCTGAAATTA AGACTGAACC TGAAGATACA ACCACAAATG CTTCTGCCCT TTCTCAGCCA
    GAGGTAGTTC CAAAGAGGGA GGAGGAGGAG GTACAGCCAC CACTGCAACA ACCACAGCGG
    GATAGAAGAG AGAGGGAAAG GGAGCGTGAA AGAGATCGTG GAAGAGAGAA GGAGAGAGGG
    GATACTTCAA AGGAGAAACC AAAGCATGAG GCAGACACTA AGCGCAAAGA GGCAGAGACG
    GTCAAACAGA TGAAGGCAGA GCTAAAGAGA GTCCAGGAAT CTCTACGAGA CATGAAGCTG
    CTATTAGATA TGTATCGTTC GGCACCAAAG GAACAAAGAG ACAAAGTGCA GCTGATGGCA
    GCGGAGAGAA AAAGTAAAGC AGAGTTGGAG GAGCTTCGTC TTCGGCTTAG AGAGCTGGAA
    GAAAGGGAAC GCAGGGACAG CAAGAAGATG GCAGACGAAG ATGCCTTACG TAGAATGAGA
    ACAGCTGAGG AGCAGACAGA GGTTCTTCAA AAAAGACTAT CAGTGGCTAA GCAGGAGGAA
    GAGGCCCTGC TTTCAGAAAT GGATGTCACA GGACAGGCAT TTGAAGACAT GCAGGAGCAA
    AATATTCGTC TTATGCAGCA ATTGAGGGAG AAGGATGATG CAAACTTTAA ACTTATGTCT
    GAGAGAATCA AATCAAACCA AATCCATAAG CTGCTCAAGG AAGAAAAGGA GGAGCTGGCA
    GACCAACTTC TTACACTGCG GACACAGGTG GATGCACAAC TCCAGGTTCT AAGGAAGCTG
    GAAGAGAAGG AACATTTACT TCAGAGTAGC ATTAATACTG GAGAGAAAGA ACTTGCTCTC
    CGAACCCAGG CTCTGGATCT GTGCAAACGT AAGACAACAG AATCCTCTCA GCAAGCAGAA
    GAATTGAGAG CTCAGCTGGA GCTGTCTCAA AAGAAGCTGC AAGATCTACG TGAGGAAATA
    ATTGAGAACA GTTCAAGTCG AGAGAAGGAT GCTTTTAATT ATAAAAGAGC TCAGGAAGAT
    ATCTCTCGCC TTCGAAAGAA ATTGGAATCT ACAAAGAAAC CTGATCTGGT GCCAACTTGC
    GATAAGATTT TGATGGAGGA AATCAAAGAG TATAAGGCCC GACTTACGTG CCCATGCTGC
    AACTCTCGTA AAATGGATGC CGTTCTAACA AAATGTTTTC ATGTGTTCTG CTTCGAGTGT
    GTTAAGACTC GCTATGATAC AAGGCAGCGA AAATGTCCCA AGTGCAATGC AGCTTTTGGA
    GCAAATGACT TCCATCGGAT TTACATTGGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa45797
    Clone ID Related Accession (Same CDS sequence) XM_018095910.1
    Accession Version XM_018095910.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase BRE1A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTCAGGGC CTGGAAGTAA ACGTACTGTG GGGGATGTAG GTTCTTTAGG ACCCCCTGAA 
    AAAAAGGCAG CAGTGGAAGA TTCTGGAACC ACTGTCGAGA CGATTAAACT AGGAAGTGTG
    TCTTCAACAG AGGAGCAAGA TCTGAGGACC CTTCAACTGA AAAACAAGAA GTTGGCTGGA
    ATGTTAGACC AGAGACAGGC CATAGAAGAT GAACTGCGTG ATCGTATTGA GACTTTAGAA
    AGGCGGCAAG CAACAGATGA TGCTTCTCTT CTCATCATAA ACCGATACTG GAGTCAGTTT
    GATGAAAATA TTGGCATTTT TTTGGGTCGT TATGACTTGG ATCAAGACTT GGGGGAGTTT
    CTGACAGAAC GCAAAGCATT GGTTCTGCCA GAGCCAGAGC CAGATTCTGA CAGCAATCCA
    GAACGCAAAG AAAATGAAAG GGGCGAAGGA CTATGGGAAG CTCCTCTTTC CTTTTTGGCC
    ACTCTGGCCA GCAGCAGCAG TGAAGAAATT GAATCTCAGC TACAAGAACG TGTAGAATCT
    TCACGACGTG CAGTCTCTCG TATTGTTTTG GTGTATGACC GACTGCATGA CCAACTGGAC
    CATCTTGCCA AGAAACTGAA TAGCACTGAT CCATCTCACG TGGAAGAGGC TGTCAGGGAT
    TTAAACTCCT TGCTCTCAAA TGAGAATGCA CGATTGCAGG AGATTTGTAA CTTGCTACAA
    GAGAAACAGC AGAATATGTC CCAAGAGTTC CTCCAGTTGC AGTCACGATT AGAGAGTGCT
    GAATCTCGAG TCTTAGTTCT AGATGGTCAC ATTGAAGATC TTCAGTGGGA CATTGATAAA
    ATTCGGAAAA GGGAACAGCG CTTAAACAGA CATTTATCTG AAGTTCTTGA GAGGGTGAAC
    TCGAAAGGTT ATAAGGTTTA TGGAGCAGGA AGCAGTCTCT ATGGGGGAAC AATCACTATA
    AATTCTCGCA AGTTTGAAGA AATGACTTCT GAAGTTGATT TGAATAAAGA GCTGGCTGTA
    AATCGACTGC AAGAGCTTGA AAAACTGAGG CAGGATTTAC AAGAAGTGAC TTCAGAAAAT
    CAGGATTTAC AGGCTGAGTT AGCCAGTGCA GTTGAAGAGA ATGTGCGGTT GTCTCCAGAG
    TATCGCTGCA TGCAGTCTCA ATTCTCTGTA TTGTACAATG AATCACTGCA GCTAAAAACT
    CAGCTAGACG AGGCTCGTTC ACTTCTACAT GGAACACGGT CCAATCATCA GCGTCAGCTA
    GAGCTTATTG AGCGTGATGA AATTTCCCTC CAGAAAAAGG TGCGCACTGA GGTTATTCAG
    TTAGAGGACA CCCTTGCTCA GGTGCGCAAG GAATATGAAA TGCTGAGGAT AGAGTTTGAG
    CAGACCCTGG CAGCCAATGA GCAAGCAGGG CCTATTAACC GAGAAATGAG ACATTTAATC
    AGCAGTCTAC AGAATCATAA TCACCAACTT AAAGGAGAGG TATTGCGGTA TAAAAGACGT
    CTGAGAGAGA TCCAAGGGGA TATAAGCAAA ATGCGTTCTC GCAGTAGCAG TAGTCTTTTC
    CTTCTGCCAT CACAGTCCAG CACTGAGGAC ACTCGTGAAG AGACAGCTGA AATTAAGACT
    GAACCTGAAG ATACAACCAC AAATGCTTCT GCCCTTTCTC AGCCAGAGGT AGTTCCAAAG
    AGGGAGGAGG AGGAGGTACA GCCACCACTG CAACAACCAC AGCGGGATAG AAGAGAGAGG
    GAAAGGGAGC GTGAAAGAGA TCGTGGAAGA GAGAAGGAGA GAGGGGATAC TTCAAAGGAG
    AAACCAAAGC ATGAGGCAGA CACTAAGCGC AAAGAGGCAG AGACGGTCAA ACAGATGAAG
    GCAGAGCTAA AGAGAGTCCA GGAATCTCTA CGAGACATGA AGCTGCTATT AGATATGTAT
    CGTTCGGCAC CAAAGGAACA AAGAGACAAA GTGCAGCTGA TGGCAGCGGA GAGAAAAAGT
    AAAGCAGAGT TGGAGGAGCT TCGTCTTCGG CTTAGAGAGC TGGAAGAAAG GGAACGCAGG
    GACAGCAAGA AGATGGCAGA CGAAGATGCC TTACGTAGAA TGAGAACAGC TGAGGAGCAG
    ACAGAGGTTC TTCAAAAAAG ACTATCAGTG GCTAAGCAGG AGGAAGAGGC CCTGCTTTCA
    GAAATGGATG TCACAGGACA GGCATTTGAA GACATGCAGG AGCAAAATAT TCGTCTTATG
    CAGCAATTGA GGGAGAAGGA TGATGCAAAC TTTAAACTTA TGTCTGAGAG AATCAAATCA
    AACCAAATCC ATAAGCTGCT CAAGGAAGAA AAGGAGGAGC TGGCAGACCA ACTTCTTACA
    CTGCGGACAC AGGTGGATGC ACAACTCCAG GTTCTAAGGA AGCTGGAAGA GAAGGAACAT
    TTACTTCAGA GTAGCATTAA TACTGGAGAG AAAGAACTTG CTCTCCGAAC CCAGGCTCTG
    GATCTGTGCA AACGTAAGAC AACAGAATCC TCTCAGCAAG CAGAAGAATT GAGAGCTCAG
    CTGGAGCTGT CTCAAAAGAA GCTGCAAGAT CTACGTGAGG AAATAATTGA GAACAGTTCA
    AGTCGAGAGA AGGATGCTTT TAATTATAAA AGAGCTCAGA AAATCTCTGC AGAGATGTAC
    ACATTTAGGA AAGGAGAAGT TGCCAAAGAC TGCTGCAGTT ACAAAGGGAA ACAGGACTTT
    TTGAGACTAT GGACAATGAA GATATCTCTC GCCTTCGAAA GAAATTGGAA TCTACAAAGA
    AACCTGATCT GGTGCCAACT TGCGATAAGA TTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951399.1
    CDS165..2960
    Translation

    Target ORF information:

    RefSeq Version XM_018095910.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger protein 20 (rnf20), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095910.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTCAGGGC CTGGAAGTAA ACGTACTGTG GGGGATGTAG GTTCTTTAGG ACCCCCTGAA 
    AAAAAGGCAG CAGTGGAAGA TTCTGGAACC ACTGTCGAGA CGATTAAACT AGGAAGTGTG
    TCTTCAACAG AGGAGCAAGA TCTGAGGACC CTTCAACTGA AAAACAAGAA GTTGGCTGGA
    ATGTTAGACC AGAGACAGGC CATAGAAGAT GAACTGCGTG ATCGTATTGA GACTTTAGAA
    AGGCGGCAAG CAACAGATGA TGCTTCTCTT CTCATCATAA ACCGATACTG GAGTCAGTTT
    GATGAAAATA TTGGCATTTT TTTGGGTCGT TATGACTTGG ATCAAGACTT GGGGGAGTTT
    CTGACAGAAC GCAAAGCATT GGTTCTGCCA GAGCCAGAGC CAGATTCTGA CAGCAATCCA
    GAACGCAAAG AAAATGAAAG GGGCGAAGGA CTATGGGAAG CTCCTCTTTC CTTTTTGGCC
    ACTCTGGCCA GCAGCAGCAG TGAAGAAATT GAATCTCAGC TACAAGAACG TGTAGAATCT
    TCACGACGTG CAGTCTCTCG TATTGTTTTG GTGTATGACC GACTGCATGA CCAACTGGAC
    CATCTTGCCA AGAAACTGAA TAGCACTGAT CCATCTCACG TGGAAGAGGC TGTCAGGGAT
    TTAAACTCCT TGCTCTCAAA TGAGAATGCA CGATTGCAGG AGATTTGTAA CTTGCTACAA
    GAGAAACAGC AGAATATGTC CCAAGAGTTC CTCCAGTTGC AGTCACGATT AGAGAGTGCT
    GAATCTCGAG TCTTAGTTCT AGATGGTCAC ATTGAAGATC TTCAGTGGGA CATTGATAAA
    ATTCGGAAAA GGGAACAGCG CTTAAACAGA CATTTATCTG AAGTTCTTGA GAGGGTGAAC
    TCGAAAGGTT ATAAGGTTTA TGGAGCAGGA AGCAGTCTCT ATGGGGGAAC AATCACTATA
    AATTCTCGCA AGTTTGAAGA AATGACTTCT GAAGTTGATT TGAATAAAGA GCTGGCTGTA
    AATCGACTGC AAGAGCTTGA AAAACTGAGG CAGGATTTAC AAGAAGTGAC TTCAGAAAAT
    CAGGATTTAC AGGCTGAGTT AGCCAGTGCA GTTGAAGAGA ATGTGCGGTT GTCTCCAGAG
    TATCGCTGCA TGCAGTCTCA ATTCTCTGTA TTGTACAATG AATCACTGCA GCTAAAAACT
    CAGCTAGACG AGGCTCGTTC ACTTCTACAT GGAACACGGT CCAATCATCA GCGTCAGCTA
    GAGCTTATTG AGCGTGATGA AATTTCCCTC CAGAAAAAGG TGCGCACTGA GGTTATTCAG
    TTAGAGGACA CCCTTGCTCA GGTGCGCAAG GAATATGAAA TGCTGAGGAT AGAGTTTGAG
    CAGACCCTGG CAGCCAATGA GCAAGCAGGG CCTATTAACC GAGAAATGAG ACATTTAATC
    AGCAGTCTAC AGAATCATAA TCACCAACTT AAAGGAGAGG TATTGCGGTA TAAAAGACGT
    CTGAGAGAGA TCCAAGGGGA TATAAGCAAA ATGCGTTCTC GCAGTAGCAG TAGTCTTTTC
    CTTCTGCCAT CACAGTCCAG CACTGAGGAC ACTCGTGAAG AGACAGCTGA AATTAAGACT
    GAACCTGAAG ATACAACCAC AAATGCTTCT GCCCTTTCTC AGCCAGAGGT AGTTCCAAAG
    AGGGAGGAGG AGGAGGTACA GCCACCACTG CAACAACCAC AGCGGGATAG AAGAGAGAGG
    GAAAGGGAGC GTGAAAGAGA TCGTGGAAGA GAGAAGGAGA GAGGGGATAC TTCAAAGGAG
    AAACCAAAGC ATGAGGCAGA CACTAAGCGC AAAGAGGCAG AGACGGTCAA ACAGATGAAG
    GCAGAGCTAA AGAGAGTCCA GGAATCTCTA CGAGACATGA AGCTGCTATT AGATATGTAT
    CGTTCGGCAC CAAAGGAACA AAGAGACAAA GTGCAGCTGA TGGCAGCGGA GAGAAAAAGT
    AAAGCAGAGT TGGAGGAGCT TCGTCTTCGG CTTAGAGAGC TGGAAGAAAG GGAACGCAGG
    GACAGCAAGA AGATGGCAGA CGAAGATGCC TTACGTAGAA TGAGAACAGC TGAGGAGCAG
    ACAGAGGTTC TTCAAAAAAG ACTATCAGTG GCTAAGCAGG AGGAAGAGGC CCTGCTTTCA
    GAAATGGATG TCACAGGACA GGCATTTGAA GACATGCAGG AGCAAAATAT TCGTCTTATG
    CAGCAATTGA GGGAGAAGGA TGATGCAAAC TTTAAACTTA TGTCTGAGAG AATCAAATCA
    AACCAAATCC ATAAGCTGCT CAAGGAAGAA AAGGAGGAGC TGGCAGACCA ACTTCTTACA
    CTGCGGACAC AGGTGGATGC ACAACTCCAG GTTCTAAGGA AGCTGGAAGA GAAGGAACAT
    TTACTTCAGA GTAGCATTAA TACTGGAGAG AAAGAACTTG CTCTCCGAAC CCAGGCTCTG
    GATCTGTGCA AACGTAAGAC AACAGAATCC TCTCAGCAAG CAGAAGAATT GAGAGCTCAG
    CTGGAGCTGT CTCAAAAGAA GCTGCAAGAT CTACGTGAGG AAATAATTGA GAACAGTTCA
    AGTCGAGAGA AGGATGCTTT TAATTATAAA AGAGCTCAGA AAATCTCTGC AGAGATGTAC
    ACATTTAGGA AAGGAGAAGT TGCCAAAGAC TGCTGCAGTT ACAAAGGGAA ACAGGACTTT
    TTGAGACTAT GGACAATGAA GATATCTCTC GCCTTCGAAA GAAATTGGAA TCTACAAAGA
    AACCTGATCT GGTGCCAACT TGCGATAAGA TTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62100
    Clone ID Related Accession (Same CDS sequence) XM_002941865.5 , XM_004910890.4 , XM_012953362.3
    Accession Version XM_002941865.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase BRE1A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002941865.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGGC CTGGAAGTAA ACGTACTGTG GGGGATGTAG GTTCTTTAGG ACCCCCTGAA 
    AAAAAGGCAG CAGTGGAAGA TTCTGGAACC ACTGTCGAGA CGATTAAACT AGGAAGTGTG
    TCTTCAACAG AGGAGCAAGA TCTGAGGACC CTTCAACTGA AAAACAAGAA GTTGGCTGGA
    ATGTTAGACC AGAGACAGGC CATAGAAGAT GAACTGCGTG ATCGTATTGA GACTTTAGAA
    AGGCGGCAAG CAACAGATGA TGCTTCTCTT CTCATCATAA ACCGATACTG GAGTCAGTTT
    GATGAAAATA TTGGCATTTT TTTGGGTCGT TATGACTTGG ATCAAGACTT GGGGGAGTTT
    CTGACAGAAC GCAAAGCATT GGTTCTGCCA GAGCCAGAGC CAGATTCTGA CAGCAATCCA
    GAACGCAAAG AAAATGAAAG GGGCGAAGGA CTATGGGAAG CTCCTCTTTC CTTTTTGGCC
    ACTCTGGCCA GCAGCAGCAG TGAAGAAATT GAATCTCAGC TACAAGAACG TGTAGAATCT
    TCACGACGTG CAGTCTCTCG TATTGTTTTG GTGTATGACC GACTGCATGA CCAACTGGAC
    CATCTTGCCA AGAAACTGAA TAGCACTGAT CCATCTCACG TGGAAGAGGC TGTCAGGGAT
    TTAAACTCCT TGCTCTCAAA TGAGAATGCA CGATTGCAGG AGATTTGTAA CTTGCTACAA
    GAGAAACAGC AGAATATGTC CCAAGAGTTC CTCCAGTTGC AGTCACGATT AGAGAGTGCT
    GAATCTCGAG TCTTAGTTCT AGATGGTCAC ATTGAAGATC TTCAGTGGGA CATTGATAAA
    ATTCGGAAAA GGGAACAGCG CTTAAACAGA CATTTATCTG AAGTTCTTGA GAGGGTGAAC
    TCGAAAGGTT ATAAGGTTTA TGGAGCAGGA AGCAGTCTCT ATGGGGGAAC AATCACTATA
    AATTCTCGCA AGTTTGAAGA AATGACTTCT GAAGTTGATT TGAATAAAGA GCTGGCTGTA
    AATCGACTGC AAGAGCTTGA AAAACTGAGG CAGGATTTAC AAGAAGTGAC TTCAGAAAAT
    CAGGATTTAC AGGCTGAGTT AGCCAGTGCA GTTGAAGAGA ATGTGCGGTT GTCTCCAGAG
    TATCGCTGCA TGCAGTCTCA ATTCTCTGTA TTGTACAATG AATCACTGCA GCTAAAAACT
    CAGCTAGACG AGGCTCGTTC ACTTCTACAT GGAACACGGT CCAATCATCA GCGTCAGCTA
    GAGCTTATTG AGCGTGATGA AATTTCCCTC CAGAAAAAGG TGCGCACTGA GGTTATTCAG
    TTAGAGGACA CCCTTGCTCA GGTGCGCAAG GAATATGAAA TGCTGAGGAT AGAGTTTGAG
    CAGACCCTGG CAGCCAATGA GCAAGCAGGG CCTATTAACC GAGAAATGAG ACATTTAATC
    AGCAGTCTAC AGAATCATAA TCACCAACTT AAAGGAGAGG TATTGCGGTA TAAAAGACGT
    CTGAGAGAGA TCCAAGGGGA TATAAGCAAA ATGCGTTCTC GCAGTAGCAG TAGTCTTTTC
    CTTCTGCCAT CACAGTCCAG CACTGAGGAC ACTCGTGAAG AGACAGCTGA AATTAAGACT
    GAACCTGAAG ATACAACCAC AAATGCTTCT GCCCTTTCTC AGCCAGAGGT AGTTCCAAAG
    AGGGAGGAGG AGGAGGTACA GCCACCACTG CAACAACCAC AGCGGGATAG AAGAGAGAGG
    GAAAGGGAGC GTGAAAGAGA TCGTGGAAGA GAGAAGGAGA GAGGGGATAC TTCAAAGGAG
    AAACCAAAGC ATGAGGCAGA CACTAAGCGC AAAGAGGCAG AGACGGTCAA ACAGATGAAG
    GCAGAGCTAA AGAGAGTCCA GGAATCTCTA CGAGACATGA AGCTGCTATT AGATATGTAT
    CGTTCGGCAC CAAAGGAACA AAGAGACAAA GTGCAGCTGA TGGCAGCGGA GAAAAAAAGT
    AAAGCAGAGT TGGAGGAGCT TCGTCTTCGG CTTAGAGAGC TGGAAGAAAG GGAACGCAGG
    GACAGCAAGA AGATGGCAGA CGAAGATGCC TTACGTAGAA TGAGAACAGC TGAGGAGCAG
    ACAGAGGTTC TTCAAAAAAG ACTATCAGTG GCTAAGCAGG AGGAAGAGGC CCTGCTTTCA
    GAAATGGATG TCACAGGACA GGCATTTGAA GACATGCAGG AGCAAAATAT TCGTCTTATG
    CAGCAATTGA GGGAGAAGGA TGATGCAAAC TTTAAACTTA TGTCTGAGAG AATCAAATCA
    AACCAAATCC ATAAGCTGCT CAAGGAAGAA AAGGAGGAGC TGGCAGACCA ACTTCTTACA
    CTGCGGACAC AGGTGGATGC ACAACTCCAG GTTCTAAGGA AGCTGGAAGA GAAGGAACAT
    TTACTTCAGA GTAGCATTAA TACTGGAGAG AAAGAACTTG CTCTCCGAAC CCAGGCTCTG
    GATCTGTGCA AACGTAAGAC AACAGAATCC TCTCAGCAAG CAGAAGAATT GAGAGCTCAG
    CTGGAGCTGT CTCAAAAGAA GCTGCAAGAT CTACGTGAGG AAATAATTGA GAACAGTTCA
    AGTCGAGAGA AGGATGCTTT TAATTATAAA AGAGCTCAGG AAGATATCTC TCGCCTTCGA
    AAGAAATTGG AATCTACAAA GAAACCTGAT CTGGTGCCAA CTTGCGATAA GATTTTGATG
    GAGGAAATCA AAGAGTATAA GGCCCGACTT ACGTGCCCAT GCTGCAACTC TCGTAAAATG
    GATGCCGTTC TAACAAAATG TTTTCATGTG TTCTGCTTCG AGTGTGTTAA GACTCGCTAT
    GATACAAGGC AGCGAAAATG TCCCAAGTGC AATGCAGCTT TTGGAGCAAA TGACTTCCAT
    CGGATTTACA TTGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002941911.2
    CDS160..3057
    Translation

    Target ORF information:

    RefSeq Version XM_002941865.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger protein 20 (rnf20), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002941865.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGGC CTGGAAGTAA ACGTACTGTG GGGGATGTAG GTTCTTTAGG ACCCCCTGAA 
    AAAAAGGCAG CAGTGGAAGA TTCTGGAACC ACTGTCGAGA CGATTAAACT AGGAAGTGTG
    TCTTCAACAG AGGAGCAAGA TCTGAGGACC CTTCAACTGA AAAACAAGAA GTTGGCTGGA
    ATGTTAGACC AGAGACAGGC CATAGAAGAT GAACTGCGTG ATCGTATTGA GACTTTAGAA
    AGGCGGCAAG CAACAGATGA TGCTTCTCTT CTCATCATAA ACCGATACTG GAGTCAGTTT
    GATGAAAATA TTGGCATTTT TTTGGGTCGT TATGACTTGG ATCAAGACTT GGGGGAGTTT
    CTGACAGAAC GCAAAGCATT GGTTCTGCCA GAGCCAGAGC CAGATTCTGA CAGCAATCCA
    GAACGCAAAG AAAATGAAAG GGGCGAAGGA CTATGGGAAG CTCCTCTTTC CTTTTTGGCC
    ACTCTGGCCA GCAGCAGCAG TGAAGAAATT GAATCTCAGC TACAAGAACG TGTAGAATCT
    TCACGACGTG CAGTCTCTCG TATTGTTTTG GTGTATGACC GACTGCATGA CCAACTGGAC
    CATCTTGCCA AGAAACTGAA TAGCACTGAT CCATCTCACG TGGAAGAGGC TGTCAGGGAT
    TTAAACTCCT TGCTCTCAAA TGAGAATGCA CGATTGCAGG AGATTTGTAA CTTGCTACAA
    GAGAAACAGC AGAATATGTC CCAAGAGTTC CTCCAGTTGC AGTCACGATT AGAGAGTGCT
    GAATCTCGAG TCTTAGTTCT AGATGGTCAC ATTGAAGATC TTCAGTGGGA CATTGATAAA
    ATTCGGAAAA GGGAACAGCG CTTAAACAGA CATTTATCTG AAGTTCTTGA GAGGGTGAAC
    TCGAAAGGTT ATAAGGTTTA TGGAGCAGGA AGCAGTCTCT ATGGGGGAAC AATCACTATA
    AATTCTCGCA AGTTTGAAGA AATGACTTCT GAAGTTGATT TGAATAAAGA GCTGGCTGTA
    AATCGACTGC AAGAGCTTGA AAAACTGAGG CAGGATTTAC AAGAAGTGAC TTCAGAAAAT
    CAGGATTTAC AGGCTGAGTT AGCCAGTGCA GTTGAAGAGA ATGTGCGGTT GTCTCCAGAG
    TATCGCTGCA TGCAGTCTCA ATTCTCTGTA TTGTACAATG AATCACTGCA GCTAAAAACT
    CAGCTAGACG AGGCTCGTTC ACTTCTACAT GGAACACGGT CCAATCATCA GCGTCAGCTA
    GAGCTTATTG AGCGTGATGA AATTTCCCTC CAGAAAAAGG TGCGCACTGA GGTTATTCAG
    TTAGAGGACA CCCTTGCTCA GGTGCGCAAG GAATATGAAA TGCTGAGGAT AGAGTTTGAG
    CAGACCCTGG CAGCCAATGA GCAAGCAGGG CCTATTAACC GAGAAATGAG ACATTTAATC
    AGCAGTCTAC AGAATCATAA TCACCAACTT AAAGGAGAGG TATTGCGGTA TAAAAGACGT
    CTGAGAGAGA TCCAAGGGGA TATAAGCAAA ATGCGTTCTC GCAGTAGCAG TAGTCTTTTC
    CTTCTGCCAT CACAGTCCAG CACTGAGGAC ACTCGTGAAG AGACAGCTGA AATTAAGACT
    GAACCTGAAG ATACAACCAC AAATGCTTCT GCCCTTTCTC AGCCAGAGGT AGTTCCAAAG
    AGGGAGGAGG AGGAGGTACA GCCACCACTG CAACAACCAC AGCGGGATAG AAGAGAGAGG
    GAAAGGGAGC GTGAAAGAGA TCGTGGAAGA GAGAAGGAGA GAGGGGATAC TTCAAAGGAG
    AAACCAAAGC ATGAGGCAGA CACTAAGCGC AAAGAGGCAG AGACGGTCAA ACAGATGAAG
    GCAGAGCTAA AGAGAGTCCA GGAATCTCTA CGAGACATGA AGCTGCTATT AGATATGTAT
    CGTTCGGCAC CAAAGGAACA AAGAGACAAA GTGCAGCTGA TGGCAGCGGA GAAAAAAAGT
    AAAGCAGAGT TGGAGGAGCT TCGTCTTCGG CTTAGAGAGC TGGAAGAAAG GGAACGCAGG
    GACAGCAAGA AGATGGCAGA CGAAGATGCC TTACGTAGAA TGAGAACAGC TGAGGAGCAG
    ACAGAGGTTC TTCAAAAAAG ACTATCAGTG GCTAAGCAGG AGGAAGAGGC CCTGCTTTCA
    GAAATGGATG TCACAGGACA GGCATTTGAA GACATGCAGG AGCAAAATAT TCGTCTTATG
    CAGCAATTGA GGGAGAAGGA TGATGCAAAC TTTAAACTTA TGTCTGAGAG AATCAAATCA
    AACCAAATCC ATAAGCTGCT CAAGGAAGAA AAGGAGGAGC TGGCAGACCA ACTTCTTACA
    CTGCGGACAC AGGTGGATGC ACAACTCCAG GTTCTAAGGA AGCTGGAAGA GAAGGAACAT
    TTACTTCAGA GTAGCATTAA TACTGGAGAG AAAGAACTTG CTCTCCGAAC CCAGGCTCTG
    GATCTGTGCA AACGTAAGAC AACAGAATCC TCTCAGCAAG CAGAAGAATT GAGAGCTCAG
    CTGGAGCTGT CTCAAAAGAA GCTGCAAGAT CTACGTGAGG AAATAATTGA GAACAGTTCA
    AGTCGAGAGA AGGATGCTTT TAATTATAAA AGAGCTCAGG AAGATATCTC TCGCCTTCGA
    AAGAAATTGG AATCTACAAA GAAACCTGAT CTGGTGCCAA CTTGCGATAA GATTTTGATG
    GAGGAAATCA AAGAGTATAA GGCCCGACTT ACGTGCCCAT GCTGCAACTC TCGTAAAATG
    GATGCCGTTC TAACAAAATG TTTTCATGTG TTCTGCTTCG AGTGTGTTAA GACTCGCTAT
    GATACAAGGC AGCGAAAATG TCCCAAGTGC AATGCAGCTT TTGGAGCAAA TGACTTCCAT
    CGGATTTACA TTGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62100
    Clone ID Related Accession (Same CDS sequence) XM_002941865.5 , XM_004910890.4 , XM_012953362.3
    Accession Version XM_004910890.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase BRE1A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910890.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGGC CTGGAAGTAA ACGTACTGTG GGGGATGTAG GTTCTTTAGG ACCCCCTGAA 
    AAAAAGGCAG CAGTGGAAGA TTCTGGAACC ACTGTCGAGA CGATTAAACT AGGAAGTGTG
    TCTTCAACAG AGGAGCAAGA TCTGAGGACC CTTCAACTGA AAAACAAGAA GTTGGCTGGA
    ATGTTAGACC AGAGACAGGC CATAGAAGAT GAACTGCGTG ATCGTATTGA GACTTTAGAA
    AGGCGGCAAG CAACAGATGA TGCTTCTCTT CTCATCATAA ACCGATACTG GAGTCAGTTT
    GATGAAAATA TTGGCATTTT TTTGGGTCGT TATGACTTGG ATCAAGACTT GGGGGAGTTT
    CTGACAGAAC GCAAAGCATT GGTTCTGCCA GAGCCAGAGC CAGATTCTGA CAGCAATCCA
    GAACGCAAAG AAAATGAAAG GGGCGAAGGA CTATGGGAAG CTCCTCTTTC CTTTTTGGCC
    ACTCTGGCCA GCAGCAGCAG TGAAGAAATT GAATCTCAGC TACAAGAACG TGTAGAATCT
    TCACGACGTG CAGTCTCTCG TATTGTTTTG GTGTATGACC GACTGCATGA CCAACTGGAC
    CATCTTGCCA AGAAACTGAA TAGCACTGAT CCATCTCACG TGGAAGAGGC TGTCAGGGAT
    TTAAACTCCT TGCTCTCAAA TGAGAATGCA CGATTGCAGG AGATTTGTAA CTTGCTACAA
    GAGAAACAGC AGAATATGTC CCAAGAGTTC CTCCAGTTGC AGTCACGATT AGAGAGTGCT
    GAATCTCGAG TCTTAGTTCT AGATGGTCAC ATTGAAGATC TTCAGTGGGA CATTGATAAA
    ATTCGGAAAA GGGAACAGCG CTTAAACAGA CATTTATCTG AAGTTCTTGA GAGGGTGAAC
    TCGAAAGGTT ATAAGGTTTA TGGAGCAGGA AGCAGTCTCT ATGGGGGAAC AATCACTATA
    AATTCTCGCA AGTTTGAAGA AATGACTTCT GAAGTTGATT TGAATAAAGA GCTGGCTGTA
    AATCGACTGC AAGAGCTTGA AAAACTGAGG CAGGATTTAC AAGAAGTGAC TTCAGAAAAT
    CAGGATTTAC AGGCTGAGTT AGCCAGTGCA GTTGAAGAGA ATGTGCGGTT GTCTCCAGAG
    TATCGCTGCA TGCAGTCTCA ATTCTCTGTA TTGTACAATG AATCACTGCA GCTAAAAACT
    CAGCTAGACG AGGCTCGTTC ACTTCTACAT GGAACACGGT CCAATCATCA GCGTCAGCTA
    GAGCTTATTG AGCGTGATGA AATTTCCCTC CAGAAAAAGG TGCGCACTGA GGTTATTCAG
    TTAGAGGACA CCCTTGCTCA GGTGCGCAAG GAATATGAAA TGCTGAGGAT AGAGTTTGAG
    CAGACCCTGG CAGCCAATGA GCAAGCAGGG CCTATTAACC GAGAAATGAG ACATTTAATC
    AGCAGTCTAC AGAATCATAA TCACCAACTT AAAGGAGAGG TATTGCGGTA TAAAAGACGT
    CTGAGAGAGA TCCAAGGGGA TATAAGCAAA ATGCGTTCTC GCAGTAGCAG TAGTCTTTTC
    CTTCTGCCAT CACAGTCCAG CACTGAGGAC ACTCGTGAAG AGACAGCTGA AATTAAGACT
    GAACCTGAAG ATACAACCAC AAATGCTTCT GCCCTTTCTC AGCCAGAGGT AGTTCCAAAG
    AGGGAGGAGG AGGAGGTACA GCCACCACTG CAACAACCAC AGCGGGATAG AAGAGAGAGG
    GAAAGGGAGC GTGAAAGAGA TCGTGGAAGA GAGAAGGAGA GAGGGGATAC TTCAAAGGAG
    AAACCAAAGC ATGAGGCAGA CACTAAGCGC AAAGAGGCAG AGACGGTCAA ACAGATGAAG
    GCAGAGCTAA AGAGAGTCCA GGAATCTCTA CGAGACATGA AGCTGCTATT AGATATGTAT
    CGTTCGGCAC CAAAGGAACA AAGAGACAAA GTGCAGCTGA TGGCAGCGGA GAAAAAAAGT
    AAAGCAGAGT TGGAGGAGCT TCGTCTTCGG CTTAGAGAGC TGGAAGAAAG GGAACGCAGG
    GACAGCAAGA AGATGGCAGA CGAAGATGCC TTACGTAGAA TGAGAACAGC TGAGGAGCAG
    ACAGAGGTTC TTCAAAAAAG ACTATCAGTG GCTAAGCAGG AGGAAGAGGC CCTGCTTTCA
    GAAATGGATG TCACAGGACA GGCATTTGAA GACATGCAGG AGCAAAATAT TCGTCTTATG
    CAGCAATTGA GGGAGAAGGA TGATGCAAAC TTTAAACTTA TGTCTGAGAG AATCAAATCA
    AACCAAATCC ATAAGCTGCT CAAGGAAGAA AAGGAGGAGC TGGCAGACCA ACTTCTTACA
    CTGCGGACAC AGGTGGATGC ACAACTCCAG GTTCTAAGGA AGCTGGAAGA GAAGGAACAT
    TTACTTCAGA GTAGCATTAA TACTGGAGAG AAAGAACTTG CTCTCCGAAC CCAGGCTCTG
    GATCTGTGCA AACGTAAGAC AACAGAATCC TCTCAGCAAG CAGAAGAATT GAGAGCTCAG
    CTGGAGCTGT CTCAAAAGAA GCTGCAAGAT CTACGTGAGG AAATAATTGA GAACAGTTCA
    AGTCGAGAGA AGGATGCTTT TAATTATAAA AGAGCTCAGG AAGATATCTC TCGCCTTCGA
    AAGAAATTGG AATCTACAAA GAAACCTGAT CTGGTGCCAA CTTGCGATAA GATTTTGATG
    GAGGAAATCA AAGAGTATAA GGCCCGACTT ACGTGCCCAT GCTGCAACTC TCGTAAAATG
    GATGCCGTTC TAACAAAATG TTTTCATGTG TTCTGCTTCG AGTGTGTTAA GACTCGCTAT
    GATACAAGGC AGCGAAAATG TCCCAAGTGC AATGCAGCTT TTGGAGCAAA TGACTTCCAT
    CGGATTTACA TTGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910947.2
    CDS123..3020
    Translation

    Target ORF information:

    RefSeq Version XM_004910890.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger protein 20 (rnf20), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910890.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGGC CTGGAAGTAA ACGTACTGTG GGGGATGTAG GTTCTTTAGG ACCCCCTGAA 
    AAAAAGGCAG CAGTGGAAGA TTCTGGAACC ACTGTCGAGA CGATTAAACT AGGAAGTGTG
    TCTTCAACAG AGGAGCAAGA TCTGAGGACC CTTCAACTGA AAAACAAGAA GTTGGCTGGA
    ATGTTAGACC AGAGACAGGC CATAGAAGAT GAACTGCGTG ATCGTATTGA GACTTTAGAA
    AGGCGGCAAG CAACAGATGA TGCTTCTCTT CTCATCATAA ACCGATACTG GAGTCAGTTT
    GATGAAAATA TTGGCATTTT TTTGGGTCGT TATGACTTGG ATCAAGACTT GGGGGAGTTT
    CTGACAGAAC GCAAAGCATT GGTTCTGCCA GAGCCAGAGC CAGATTCTGA CAGCAATCCA
    GAACGCAAAG AAAATGAAAG GGGCGAAGGA CTATGGGAAG CTCCTCTTTC CTTTTTGGCC
    ACTCTGGCCA GCAGCAGCAG TGAAGAAATT GAATCTCAGC TACAAGAACG TGTAGAATCT
    TCACGACGTG CAGTCTCTCG TATTGTTTTG GTGTATGACC GACTGCATGA CCAACTGGAC
    CATCTTGCCA AGAAACTGAA TAGCACTGAT CCATCTCACG TGGAAGAGGC TGTCAGGGAT
    TTAAACTCCT TGCTCTCAAA TGAGAATGCA CGATTGCAGG AGATTTGTAA CTTGCTACAA
    GAGAAACAGC AGAATATGTC CCAAGAGTTC CTCCAGTTGC AGTCACGATT AGAGAGTGCT
    GAATCTCGAG TCTTAGTTCT AGATGGTCAC ATTGAAGATC TTCAGTGGGA CATTGATAAA
    ATTCGGAAAA GGGAACAGCG CTTAAACAGA CATTTATCTG AAGTTCTTGA GAGGGTGAAC
    TCGAAAGGTT ATAAGGTTTA TGGAGCAGGA AGCAGTCTCT ATGGGGGAAC AATCACTATA
    AATTCTCGCA AGTTTGAAGA AATGACTTCT GAAGTTGATT TGAATAAAGA GCTGGCTGTA
    AATCGACTGC AAGAGCTTGA AAAACTGAGG CAGGATTTAC AAGAAGTGAC TTCAGAAAAT
    CAGGATTTAC AGGCTGAGTT AGCCAGTGCA GTTGAAGAGA ATGTGCGGTT GTCTCCAGAG
    TATCGCTGCA TGCAGTCTCA ATTCTCTGTA TTGTACAATG AATCACTGCA GCTAAAAACT
    CAGCTAGACG AGGCTCGTTC ACTTCTACAT GGAACACGGT CCAATCATCA GCGTCAGCTA
    GAGCTTATTG AGCGTGATGA AATTTCCCTC CAGAAAAAGG TGCGCACTGA GGTTATTCAG
    TTAGAGGACA CCCTTGCTCA GGTGCGCAAG GAATATGAAA TGCTGAGGAT AGAGTTTGAG
    CAGACCCTGG CAGCCAATGA GCAAGCAGGG CCTATTAACC GAGAAATGAG ACATTTAATC
    AGCAGTCTAC AGAATCATAA TCACCAACTT AAAGGAGAGG TATTGCGGTA TAAAAGACGT
    CTGAGAGAGA TCCAAGGGGA TATAAGCAAA ATGCGTTCTC GCAGTAGCAG TAGTCTTTTC
    CTTCTGCCAT CACAGTCCAG CACTGAGGAC ACTCGTGAAG AGACAGCTGA AATTAAGACT
    GAACCTGAAG ATACAACCAC AAATGCTTCT GCCCTTTCTC AGCCAGAGGT AGTTCCAAAG
    AGGGAGGAGG AGGAGGTACA GCCACCACTG CAACAACCAC AGCGGGATAG AAGAGAGAGG
    GAAAGGGAGC GTGAAAGAGA TCGTGGAAGA GAGAAGGAGA GAGGGGATAC TTCAAAGGAG
    AAACCAAAGC ATGAGGCAGA CACTAAGCGC AAAGAGGCAG AGACGGTCAA ACAGATGAAG
    GCAGAGCTAA AGAGAGTCCA GGAATCTCTA CGAGACATGA AGCTGCTATT AGATATGTAT
    CGTTCGGCAC CAAAGGAACA AAGAGACAAA GTGCAGCTGA TGGCAGCGGA GAAAAAAAGT
    AAAGCAGAGT TGGAGGAGCT TCGTCTTCGG CTTAGAGAGC TGGAAGAAAG GGAACGCAGG
    GACAGCAAGA AGATGGCAGA CGAAGATGCC TTACGTAGAA TGAGAACAGC TGAGGAGCAG
    ACAGAGGTTC TTCAAAAAAG ACTATCAGTG GCTAAGCAGG AGGAAGAGGC CCTGCTTTCA
    GAAATGGATG TCACAGGACA GGCATTTGAA GACATGCAGG AGCAAAATAT TCGTCTTATG
    CAGCAATTGA GGGAGAAGGA TGATGCAAAC TTTAAACTTA TGTCTGAGAG AATCAAATCA
    AACCAAATCC ATAAGCTGCT CAAGGAAGAA AAGGAGGAGC TGGCAGACCA ACTTCTTACA
    CTGCGGACAC AGGTGGATGC ACAACTCCAG GTTCTAAGGA AGCTGGAAGA GAAGGAACAT
    TTACTTCAGA GTAGCATTAA TACTGGAGAG AAAGAACTTG CTCTCCGAAC CCAGGCTCTG
    GATCTGTGCA AACGTAAGAC AACAGAATCC TCTCAGCAAG CAGAAGAATT GAGAGCTCAG
    CTGGAGCTGT CTCAAAAGAA GCTGCAAGAT CTACGTGAGG AAATAATTGA GAACAGTTCA
    AGTCGAGAGA AGGATGCTTT TAATTATAAA AGAGCTCAGG AAGATATCTC TCGCCTTCGA
    AAGAAATTGG AATCTACAAA GAAACCTGAT CTGGTGCCAA CTTGCGATAA GATTTTGATG
    GAGGAAATCA AAGAGTATAA GGCCCGACTT ACGTGCCCAT GCTGCAACTC TCGTAAAATG
    GATGCCGTTC TAACAAAATG TTTTCATGTG TTCTGCTTCG AGTGTGTTAA GACTCGCTAT
    GATACAAGGC AGCGAAAATG TCCCAAGTGC AATGCAGCTT TTGGAGCAAA TGACTTCCAT
    CGGATTTACA TTGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62100
    Clone ID Related Accession (Same CDS sequence) XM_002941865.5 , XM_004910890.4 , XM_012953362.3
    Accession Version XM_012953362.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase BRE1A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012953362.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGGC CTGGAAGTAA ACGTACTGTG GGGGATGTAG GTTCTTTAGG ACCCCCTGAA 
    AAAAAGGCAG CAGTGGAAGA TTCTGGAACC ACTGTCGAGA CGATTAAACT AGGAAGTGTG
    TCTTCAACAG AGGAGCAAGA TCTGAGGACC CTTCAACTGA AAAACAAGAA GTTGGCTGGA
    ATGTTAGACC AGAGACAGGC CATAGAAGAT GAACTGCGTG ATCGTATTGA GACTTTAGAA
    AGGCGGCAAG CAACAGATGA TGCTTCTCTT CTCATCATAA ACCGATACTG GAGTCAGTTT
    GATGAAAATA TTGGCATTTT TTTGGGTCGT TATGACTTGG ATCAAGACTT GGGGGAGTTT
    CTGACAGAAC GCAAAGCATT GGTTCTGCCA GAGCCAGAGC CAGATTCTGA CAGCAATCCA
    GAACGCAAAG AAAATGAAAG GGGCGAAGGA CTATGGGAAG CTCCTCTTTC CTTTTTGGCC
    ACTCTGGCCA GCAGCAGCAG TGAAGAAATT GAATCTCAGC TACAAGAACG TGTAGAATCT
    TCACGACGTG CAGTCTCTCG TATTGTTTTG GTGTATGACC GACTGCATGA CCAACTGGAC
    CATCTTGCCA AGAAACTGAA TAGCACTGAT CCATCTCACG TGGAAGAGGC TGTCAGGGAT
    TTAAACTCCT TGCTCTCAAA TGAGAATGCA CGATTGCAGG AGATTTGTAA CTTGCTACAA
    GAGAAACAGC AGAATATGTC CCAAGAGTTC CTCCAGTTGC AGTCACGATT AGAGAGTGCT
    GAATCTCGAG TCTTAGTTCT AGATGGTCAC ATTGAAGATC TTCAGTGGGA CATTGATAAA
    ATTCGGAAAA GGGAACAGCG CTTAAACAGA CATTTATCTG AAGTTCTTGA GAGGGTGAAC
    TCGAAAGGTT ATAAGGTTTA TGGAGCAGGA AGCAGTCTCT ATGGGGGAAC AATCACTATA
    AATTCTCGCA AGTTTGAAGA AATGACTTCT GAAGTTGATT TGAATAAAGA GCTGGCTGTA
    AATCGACTGC AAGAGCTTGA AAAACTGAGG CAGGATTTAC AAGAAGTGAC TTCAGAAAAT
    CAGGATTTAC AGGCTGAGTT AGCCAGTGCA GTTGAAGAGA ATGTGCGGTT GTCTCCAGAG
    TATCGCTGCA TGCAGTCTCA ATTCTCTGTA TTGTACAATG AATCACTGCA GCTAAAAACT
    CAGCTAGACG AGGCTCGTTC ACTTCTACAT GGAACACGGT CCAATCATCA GCGTCAGCTA
    GAGCTTATTG AGCGTGATGA AATTTCCCTC CAGAAAAAGG TGCGCACTGA GGTTATTCAG
    TTAGAGGACA CCCTTGCTCA GGTGCGCAAG GAATATGAAA TGCTGAGGAT AGAGTTTGAG
    CAGACCCTGG CAGCCAATGA GCAAGCAGGG CCTATTAACC GAGAAATGAG ACATTTAATC
    AGCAGTCTAC AGAATCATAA TCACCAACTT AAAGGAGAGG TATTGCGGTA TAAAAGACGT
    CTGAGAGAGA TCCAAGGGGA TATAAGCAAA ATGCGTTCTC GCAGTAGCAG TAGTCTTTTC
    CTTCTGCCAT CACAGTCCAG CACTGAGGAC ACTCGTGAAG AGACAGCTGA AATTAAGACT
    GAACCTGAAG ATACAACCAC AAATGCTTCT GCCCTTTCTC AGCCAGAGGT AGTTCCAAAG
    AGGGAGGAGG AGGAGGTACA GCCACCACTG CAACAACCAC AGCGGGATAG AAGAGAGAGG
    GAAAGGGAGC GTGAAAGAGA TCGTGGAAGA GAGAAGGAGA GAGGGGATAC TTCAAAGGAG
    AAACCAAAGC ATGAGGCAGA CACTAAGCGC AAAGAGGCAG AGACGGTCAA ACAGATGAAG
    GCAGAGCTAA AGAGAGTCCA GGAATCTCTA CGAGACATGA AGCTGCTATT AGATATGTAT
    CGTTCGGCAC CAAAGGAACA AAGAGACAAA GTGCAGCTGA TGGCAGCGGA GAAAAAAAGT
    AAAGCAGAGT TGGAGGAGCT TCGTCTTCGG CTTAGAGAGC TGGAAGAAAG GGAACGCAGG
    GACAGCAAGA AGATGGCAGA CGAAGATGCC TTACGTAGAA TGAGAACAGC TGAGGAGCAG
    ACAGAGGTTC TTCAAAAAAG ACTATCAGTG GCTAAGCAGG AGGAAGAGGC CCTGCTTTCA
    GAAATGGATG TCACAGGACA GGCATTTGAA GACATGCAGG AGCAAAATAT TCGTCTTATG
    CAGCAATTGA GGGAGAAGGA TGATGCAAAC TTTAAACTTA TGTCTGAGAG AATCAAATCA
    AACCAAATCC ATAAGCTGCT CAAGGAAGAA AAGGAGGAGC TGGCAGACCA ACTTCTTACA
    CTGCGGACAC AGGTGGATGC ACAACTCCAG GTTCTAAGGA AGCTGGAAGA GAAGGAACAT
    TTACTTCAGA GTAGCATTAA TACTGGAGAG AAAGAACTTG CTCTCCGAAC CCAGGCTCTG
    GATCTGTGCA AACGTAAGAC AACAGAATCC TCTCAGCAAG CAGAAGAATT GAGAGCTCAG
    CTGGAGCTGT CTCAAAAGAA GCTGCAAGAT CTACGTGAGG AAATAATTGA GAACAGTTCA
    AGTCGAGAGA AGGATGCTTT TAATTATAAA AGAGCTCAGG AAGATATCTC TCGCCTTCGA
    AAGAAATTGG AATCTACAAA GAAACCTGAT CTGGTGCCAA CTTGCGATAA GATTTTGATG
    GAGGAAATCA AAGAGTATAA GGCCCGACTT ACGTGCCCAT GCTGCAACTC TCGTAAAATG
    GATGCCGTTC TAACAAAATG TTTTCATGTG TTCTGCTTCG AGTGTGTTAA GACTCGCTAT
    GATACAAGGC AGCGAAAATG TCCCAAGTGC AATGCAGCTT TTGGAGCAAA TGACTTCCAT
    CGGATTTACA TTGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012808816.2
    CDS115..3012
    Translation

    Target ORF information:

    RefSeq Version XM_012953362.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger protein 20 (rnf20), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012953362.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGGC CTGGAAGTAA ACGTACTGTG GGGGATGTAG GTTCTTTAGG ACCCCCTGAA 
    AAAAAGGCAG CAGTGGAAGA TTCTGGAACC ACTGTCGAGA CGATTAAACT AGGAAGTGTG
    TCTTCAACAG AGGAGCAAGA TCTGAGGACC CTTCAACTGA AAAACAAGAA GTTGGCTGGA
    ATGTTAGACC AGAGACAGGC CATAGAAGAT GAACTGCGTG ATCGTATTGA GACTTTAGAA
    AGGCGGCAAG CAACAGATGA TGCTTCTCTT CTCATCATAA ACCGATACTG GAGTCAGTTT
    GATGAAAATA TTGGCATTTT TTTGGGTCGT TATGACTTGG ATCAAGACTT GGGGGAGTTT
    CTGACAGAAC GCAAAGCATT GGTTCTGCCA GAGCCAGAGC CAGATTCTGA CAGCAATCCA
    GAACGCAAAG AAAATGAAAG GGGCGAAGGA CTATGGGAAG CTCCTCTTTC CTTTTTGGCC
    ACTCTGGCCA GCAGCAGCAG TGAAGAAATT GAATCTCAGC TACAAGAACG TGTAGAATCT
    TCACGACGTG CAGTCTCTCG TATTGTTTTG GTGTATGACC GACTGCATGA CCAACTGGAC
    CATCTTGCCA AGAAACTGAA TAGCACTGAT CCATCTCACG TGGAAGAGGC TGTCAGGGAT
    TTAAACTCCT TGCTCTCAAA TGAGAATGCA CGATTGCAGG AGATTTGTAA CTTGCTACAA
    GAGAAACAGC AGAATATGTC CCAAGAGTTC CTCCAGTTGC AGTCACGATT AGAGAGTGCT
    GAATCTCGAG TCTTAGTTCT AGATGGTCAC ATTGAAGATC TTCAGTGGGA CATTGATAAA
    ATTCGGAAAA GGGAACAGCG CTTAAACAGA CATTTATCTG AAGTTCTTGA GAGGGTGAAC
    TCGAAAGGTT ATAAGGTTTA TGGAGCAGGA AGCAGTCTCT ATGGGGGAAC AATCACTATA
    AATTCTCGCA AGTTTGAAGA AATGACTTCT GAAGTTGATT TGAATAAAGA GCTGGCTGTA
    AATCGACTGC AAGAGCTTGA AAAACTGAGG CAGGATTTAC AAGAAGTGAC TTCAGAAAAT
    CAGGATTTAC AGGCTGAGTT AGCCAGTGCA GTTGAAGAGA ATGTGCGGTT GTCTCCAGAG
    TATCGCTGCA TGCAGTCTCA ATTCTCTGTA TTGTACAATG AATCACTGCA GCTAAAAACT
    CAGCTAGACG AGGCTCGTTC ACTTCTACAT GGAACACGGT CCAATCATCA GCGTCAGCTA
    GAGCTTATTG AGCGTGATGA AATTTCCCTC CAGAAAAAGG TGCGCACTGA GGTTATTCAG
    TTAGAGGACA CCCTTGCTCA GGTGCGCAAG GAATATGAAA TGCTGAGGAT AGAGTTTGAG
    CAGACCCTGG CAGCCAATGA GCAAGCAGGG CCTATTAACC GAGAAATGAG ACATTTAATC
    AGCAGTCTAC AGAATCATAA TCACCAACTT AAAGGAGAGG TATTGCGGTA TAAAAGACGT
    CTGAGAGAGA TCCAAGGGGA TATAAGCAAA ATGCGTTCTC GCAGTAGCAG TAGTCTTTTC
    CTTCTGCCAT CACAGTCCAG CACTGAGGAC ACTCGTGAAG AGACAGCTGA AATTAAGACT
    GAACCTGAAG ATACAACCAC AAATGCTTCT GCCCTTTCTC AGCCAGAGGT AGTTCCAAAG
    AGGGAGGAGG AGGAGGTACA GCCACCACTG CAACAACCAC AGCGGGATAG AAGAGAGAGG
    GAAAGGGAGC GTGAAAGAGA TCGTGGAAGA GAGAAGGAGA GAGGGGATAC TTCAAAGGAG
    AAACCAAAGC ATGAGGCAGA CACTAAGCGC AAAGAGGCAG AGACGGTCAA ACAGATGAAG
    GCAGAGCTAA AGAGAGTCCA GGAATCTCTA CGAGACATGA AGCTGCTATT AGATATGTAT
    CGTTCGGCAC CAAAGGAACA AAGAGACAAA GTGCAGCTGA TGGCAGCGGA GAAAAAAAGT
    AAAGCAGAGT TGGAGGAGCT TCGTCTTCGG CTTAGAGAGC TGGAAGAAAG GGAACGCAGG
    GACAGCAAGA AGATGGCAGA CGAAGATGCC TTACGTAGAA TGAGAACAGC TGAGGAGCAG
    ACAGAGGTTC TTCAAAAAAG ACTATCAGTG GCTAAGCAGG AGGAAGAGGC CCTGCTTTCA
    GAAATGGATG TCACAGGACA GGCATTTGAA GACATGCAGG AGCAAAATAT TCGTCTTATG
    CAGCAATTGA GGGAGAAGGA TGATGCAAAC TTTAAACTTA TGTCTGAGAG AATCAAATCA
    AACCAAATCC ATAAGCTGCT CAAGGAAGAA AAGGAGGAGC TGGCAGACCA ACTTCTTACA
    CTGCGGACAC AGGTGGATGC ACAACTCCAG GTTCTAAGGA AGCTGGAAGA GAAGGAACAT
    TTACTTCAGA GTAGCATTAA TACTGGAGAG AAAGAACTTG CTCTCCGAAC CCAGGCTCTG
    GATCTGTGCA AACGTAAGAC AACAGAATCC TCTCAGCAAG CAGAAGAATT GAGAGCTCAG
    CTGGAGCTGT CTCAAAAGAA GCTGCAAGAT CTACGTGAGG AAATAATTGA GAACAGTTCA
    AGTCGAGAGA AGGATGCTTT TAATTATAAA AGAGCTCAGG AAGATATCTC TCGCCTTCGA
    AAGAAATTGG AATCTACAAA GAAACCTGAT CTGGTGCCAA CTTGCGATAA GATTTTGATG
    GAGGAAATCA AAGAGTATAA GGCCCGACTT ACGTGCCCAT GCTGCAACTC TCGTAAAATG
    GATGCCGTTC TAACAAAATG TTTTCATGTG TTCTGCTTCG AGTGTGTTAA GACTCGCTAT
    GATACAAGGC AGCGAAAATG TCCCAAGTGC AATGCAGCTT TTGGAGCAAA TGACTTCCAT
    CGGATTTACA TTGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62101
    Clone ID Related Accession (Same CDS sequence) XM_004910891.4
    Accession Version XM_004910891.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2853bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase BRE1A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910891.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTCAGGGC CTGGAAGTAA ACGTACTGTG GGGGATGTAG GTTCTTTAGG ACCCCCTGAA 
    AAAAAGGCAG CAGTGGAAGA TTCTGGAACC ACTGTCGAGA CGATTAAACT AGGAAGTGTG
    TCTTCAACAG AGGAGCAAGA TCTGAGGACC CTTCAACTGA AAAACAAGAA GTTGGCTGGA
    ATGTTAGACC AGAGACAGGC CATAGAAGAT GAACTGCGTG ATCGTATTGA GACTTTAGAA
    AGGCGGCAAG CAACAGATGA TGCTTCTCTT CTCATCATAA ACCGATACTG GAGTCAGTTT
    GATGAAAATA TTGGCATTTT TTTGGGTCGT TATGACTTGG ATCAAGACTT GGGGGAGTTT
    CTGACAGAAC GCAAAGCATT GGTTCTGCCA GAGCCAGAGC CAGATTCTGA CAGCAATCCA
    GAACGCAAAG AAAATGAAAG GGGCGAAGGA CTATGGGAAG CTCCTCTTTC CTTTTTGGCC
    ACTCTGGCCA GCAGCAGCAG TGAAGAAATT GAATCTCAGC TACAAGAACG TGTAGAATCT
    TCACGACGTG CAGTCTCTCG TATTGTTTTG GTGTATGACC GACTGCATGA CCAACTGGAC
    CATCTTGCCA AGAAACTGAA TAGCACTGAT CCATCTCACG TGGAAGAGGC TGTCAGGGAT
    TTAAACTCCT TGCTCTCAAA TGAGAATGCA CGATTGCAGG AGATTTGTAA CTTGCTACAA
    GAGAAACAGC AGAATATGTC CCAAGAGTTC CTCCAGTTGC AGTCACGATT AGAGAGTGCT
    GAATCTCGAG TCTTAGTTCT AGATGGTCAC ATTGAAGATC TTCAGTGGGA CATTGATAAA
    ATTCGGAAAA GGGAACAGCG CTTAAACAGA CATTTATCTG AAGTTCTTGA GAGGGTGAAC
    TCGAAAGGTT ATAAGGTTTA TGGAGCAGGA AGCAGTCTCT ATGGGGGAAC AATCACTATA
    AATTCTCGCA AGTTTGAAGA AATGACTTCT GAAGTTGATT TGAATAAAGA GCTGGCTGTA
    AATCGACTGC AAGAGCTTGA AAAACTGAGG CAGGATTTAC AAGAAGTGAC TTCAGAAAAT
    CAGGATTTAC AGGCTGAGTT AGCCAGTGCA GTTGAAGAGA ATGTGCGGTT GTCTCCAGAG
    TATCGCTGCA TGCAGTCTCA ATTCTCTGTA TTGTACAATG AATCACTGCA GCTAAAAACT
    CAGCTAGACG AGGCTCGTTC ACTTCTACAT GGAACACGGT CCAATCATCA GCGTCAGCTA
    GAGCTTATTG AGCGTGATGA AATTTCCCTC CAGAAAAAGG TGCGCACTGA GGTTATTCAG
    TTAGAGGACA CCCTTGCTCA GGTGCGCAAG GAATATGAAA TGCTGAGGAT AGAGTTTGAG
    CAGACCCTGG CAGCCAATGA GCAAGCAGGG CCTATTAACC GAGAAATGAG ACATTTAATC
    AGCAGTCTAC AGAATCATAA TCACCAACTT AAAGGAGAGG TATTGCGGTA TAAAAGACGT
    CTGAGAGAGA TCCAAGGGGA TATAAGCAAA TCCAGCACTG AGGACACTCG TGAAGAGACA
    GCTGAAATTA AGACTGAACC TGAAGATACA ACCACAAATG CTTCTGCCCT TTCTCAGCCA
    GAGGTAGTTC CAAAGAGGGA GGAGGAGGAG GTACAGCCAC CACTGCAACA ACCACAGCGG
    GATAGAAGAG AGAGGGAAAG GGAGCGTGAA AGAGATCGTG GAAGAGAGAA GGAGAGAGGG
    GATACTTCAA AGGAGAAACC AAAGCATGAG GCAGACACTA AGCGCAAAGA GGCAGAGACG
    GTCAAACAGA TGAAGGCAGA GCTAAAGAGA GTCCAGGAAT CTCTACGAGA CATGAAGCTG
    CTATTAGATA TGTATCGTTC GGCACCAAAG GAACAAAGAG ACAAAGTGCA GCTGATGGCA
    GCGGAGAAAA AAAGTAAAGC AGAGTTGGAG GAGCTTCGTC TTCGGCTTAG AGAGCTGGAA
    GAAAGGGAAC GCAGGGACAG CAAGAAGATG GCAGACGAAG ATGCCTTACG TAGAATGAGA
    ACAGCTGAGG AGCAGACAGA GGTTCTTCAA AAAAGACTAT CAGTGGCTAA GCAGGAGGAA
    GAGGCCCTGC TTTCAGAAAT GGATGTCACA GGACAGGCAT TTGAAGACAT GCAGGAGCAA
    AATATTCGTC TTATGCAGCA ATTGAGGGAG AAGGATGATG CAAACTTTAA ACTTATGTCT
    GAGAGAATCA AATCAAACCA AATCCATAAG CTGCTCAAGG AAGAAAAGGA GGAGCTGGCA
    GACCAACTTC TTACACTGCG GACACAGGTG GATGCACAAC TCCAGGTTCT AAGGAAGCTG
    GAAGAGAAGG AACATTTACT TCAGAGTAGC ATTAATACTG GAGAGAAAGA ACTTGCTCTC
    CGAACCCAGG CTCTGGATCT GTGCAAACGT AAGACAACAG AATCCTCTCA GCAAGCAGAA
    GAATTGAGAG CTCAGCTGGA GCTGTCTCAA AAGAAGCTGC AAGATCTACG TGAGGAAATA
    ATTGAGAACA GTTCAAGTCG AGAGAAGGAT GCTTTTAATT ATAAAAGAGC TCAGGAAGAT
    ATCTCTCGCC TTCGAAAGAA ATTGGAATCT ACAAAGAAAC CTGATCTGGT GCCAACTTGC
    GATAAGATTT TGATGGAGGA AATCAAAGAG TATAAGGCCC GACTTACGTG CCCATGCTGC
    AACTCTCGTA AAATGGATGC CGTTCTAACA AAATGTTTTC ATGTGTTCTG CTTCGAGTGT
    GTTAAGACTC GCTATGATAC AAGGCAGCGA AAATGTCCCA AGTGCAATGC AGCTTTTGGA
    GCAAATGACT TCCATCGGAT TTACATTGGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910948.2
    CDS160..3012
    Translation

    Target ORF information:

    RefSeq Version XM_004910891.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger protein 20 (rnf20), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910891.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTCAGGGC CTGGAAGTAA ACGTACTGTG GGGGATGTAG GTTCTTTAGG ACCCCCTGAA 
    AAAAAGGCAG CAGTGGAAGA TTCTGGAACC ACTGTCGAGA CGATTAAACT AGGAAGTGTG
    TCTTCAACAG AGGAGCAAGA TCTGAGGACC CTTCAACTGA AAAACAAGAA GTTGGCTGGA
    ATGTTAGACC AGAGACAGGC CATAGAAGAT GAACTGCGTG ATCGTATTGA GACTTTAGAA
    AGGCGGCAAG CAACAGATGA TGCTTCTCTT CTCATCATAA ACCGATACTG GAGTCAGTTT
    GATGAAAATA TTGGCATTTT TTTGGGTCGT TATGACTTGG ATCAAGACTT GGGGGAGTTT
    CTGACAGAAC GCAAAGCATT GGTTCTGCCA GAGCCAGAGC CAGATTCTGA CAGCAATCCA
    GAACGCAAAG AAAATGAAAG GGGCGAAGGA CTATGGGAAG CTCCTCTTTC CTTTTTGGCC
    ACTCTGGCCA GCAGCAGCAG TGAAGAAATT GAATCTCAGC TACAAGAACG TGTAGAATCT
    TCACGACGTG CAGTCTCTCG TATTGTTTTG GTGTATGACC GACTGCATGA CCAACTGGAC
    CATCTTGCCA AGAAACTGAA TAGCACTGAT CCATCTCACG TGGAAGAGGC TGTCAGGGAT
    TTAAACTCCT TGCTCTCAAA TGAGAATGCA CGATTGCAGG AGATTTGTAA CTTGCTACAA
    GAGAAACAGC AGAATATGTC CCAAGAGTTC CTCCAGTTGC AGTCACGATT AGAGAGTGCT
    GAATCTCGAG TCTTAGTTCT AGATGGTCAC ATTGAAGATC TTCAGTGGGA CATTGATAAA
    ATTCGGAAAA GGGAACAGCG CTTAAACAGA CATTTATCTG AAGTTCTTGA GAGGGTGAAC
    TCGAAAGGTT ATAAGGTTTA TGGAGCAGGA AGCAGTCTCT ATGGGGGAAC AATCACTATA
    AATTCTCGCA AGTTTGAAGA AATGACTTCT GAAGTTGATT TGAATAAAGA GCTGGCTGTA
    AATCGACTGC AAGAGCTTGA AAAACTGAGG CAGGATTTAC AAGAAGTGAC TTCAGAAAAT
    CAGGATTTAC AGGCTGAGTT AGCCAGTGCA GTTGAAGAGA ATGTGCGGTT GTCTCCAGAG
    TATCGCTGCA TGCAGTCTCA ATTCTCTGTA TTGTACAATG AATCACTGCA GCTAAAAACT
    CAGCTAGACG AGGCTCGTTC ACTTCTACAT GGAACACGGT CCAATCATCA GCGTCAGCTA
    GAGCTTATTG AGCGTGATGA AATTTCCCTC CAGAAAAAGG TGCGCACTGA GGTTATTCAG
    TTAGAGGACA CCCTTGCTCA GGTGCGCAAG GAATATGAAA TGCTGAGGAT AGAGTTTGAG
    CAGACCCTGG CAGCCAATGA GCAAGCAGGG CCTATTAACC GAGAAATGAG ACATTTAATC
    AGCAGTCTAC AGAATCATAA TCACCAACTT AAAGGAGAGG TATTGCGGTA TAAAAGACGT
    CTGAGAGAGA TCCAAGGGGA TATAAGCAAA TCCAGCACTG AGGACACTCG TGAAGAGACA
    GCTGAAATTA AGACTGAACC TGAAGATACA ACCACAAATG CTTCTGCCCT TTCTCAGCCA
    GAGGTAGTTC CAAAGAGGGA GGAGGAGGAG GTACAGCCAC CACTGCAACA ACCACAGCGG
    GATAGAAGAG AGAGGGAAAG GGAGCGTGAA AGAGATCGTG GAAGAGAGAA GGAGAGAGGG
    GATACTTCAA AGGAGAAACC AAAGCATGAG GCAGACACTA AGCGCAAAGA GGCAGAGACG
    GTCAAACAGA TGAAGGCAGA GCTAAAGAGA GTCCAGGAAT CTCTACGAGA CATGAAGCTG
    CTATTAGATA TGTATCGTTC GGCACCAAAG GAACAAAGAG ACAAAGTGCA GCTGATGGCA
    GCGGAGAAAA AAAGTAAAGC AGAGTTGGAG GAGCTTCGTC TTCGGCTTAG AGAGCTGGAA
    GAAAGGGAAC GCAGGGACAG CAAGAAGATG GCAGACGAAG ATGCCTTACG TAGAATGAGA
    ACAGCTGAGG AGCAGACAGA GGTTCTTCAA AAAAGACTAT CAGTGGCTAA GCAGGAGGAA
    GAGGCCCTGC TTTCAGAAAT GGATGTCACA GGACAGGCAT TTGAAGACAT GCAGGAGCAA
    AATATTCGTC TTATGCAGCA ATTGAGGGAG AAGGATGATG CAAACTTTAA ACTTATGTCT
    GAGAGAATCA AATCAAACCA AATCCATAAG CTGCTCAAGG AAGAAAAGGA GGAGCTGGCA
    GACCAACTTC TTACACTGCG GACACAGGTG GATGCACAAC TCCAGGTTCT AAGGAAGCTG
    GAAGAGAAGG AACATTTACT TCAGAGTAGC ATTAATACTG GAGAGAAAGA ACTTGCTCTC
    CGAACCCAGG CTCTGGATCT GTGCAAACGT AAGACAACAG AATCCTCTCA GCAAGCAGAA
    GAATTGAGAG CTCAGCTGGA GCTGTCTCAA AAGAAGCTGC AAGATCTACG TGAGGAAATA
    ATTGAGAACA GTTCAAGTCG AGAGAAGGAT GCTTTTAATT ATAAAAGAGC TCAGGAAGAT
    ATCTCTCGCC TTCGAAAGAA ATTGGAATCT ACAAAGAAAC CTGATCTGGT GCCAACTTGC
    GATAAGATTT TGATGGAGGA AATCAAAGAG TATAAGGCCC GACTTACGTG CCCATGCTGC
    AACTCTCGTA AAATGGATGC CGTTCTAACA AAATGTTTTC ATGTGTTCTG CTTCGAGTGT
    GTTAAGACTC GCTATGATAC AAGGCAGCGA AAATGTCCCA AGTGCAATGC AGCTTTTGGA
    GCAAATGACT TCCATCGGAT TTACATTGGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62102
    Clone ID Related Accession (Same CDS sequence) XM_018095910.2
    Accession Version XM_018095910.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase BRE1A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018095910.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTCAGGGC CTGGAAGTAA ACGTACTGTG GGGGATGTAG GTTCTTTAGG ACCCCCTGAA 
    AAAAAGGCAG CAGTGGAAGA TTCTGGAACC ACTGTCGAGA CGATTAAACT AGGAAGTGTG
    TCTTCAACAG AGGAGCAAGA TCTGAGGACC CTTCAACTGA AAAACAAGAA GTTGGCTGGA
    ATGTTAGACC AGAGACAGGC CATAGAAGAT GAACTGCGTG ATCGTATTGA GACTTTAGAA
    AGGCGGCAAG CAACAGATGA TGCTTCTCTT CTCATCATAA ACCGATACTG GAGTCAGTTT
    GATGAAAATA TTGGCATTTT TTTGGGTCGT TATGACTTGG ATCAAGACTT GGGGGAGTTT
    CTGACAGAAC GCAAAGCATT GGTTCTGCCA GAGCCAGAGC CAGATTCTGA CAGCAATCCA
    GAACGCAAAG AAAATGAAAG GGGCGAAGGA CTATGGGAAG CTCCTCTTTC CTTTTTGGCC
    ACTCTGGCCA GCAGCAGCAG TGAAGAAATT GAATCTCAGC TACAAGAACG TGTAGAATCT
    TCACGACGTG CAGTCTCTCG TATTGTTTTG GTGTATGACC GACTGCATGA CCAACTGGAC
    CATCTTGCCA AGAAACTGAA TAGCACTGAT CCATCTCACG TGGAAGAGGC TGTCAGGGAT
    TTAAACTCCT TGCTCTCAAA TGAGAATGCA CGATTGCAGG AGATTTGTAA CTTGCTACAA
    GAGAAACAGC AGAATATGTC CCAAGAGTTC CTCCAGTTGC AGTCACGATT AGAGAGTGCT
    GAATCTCGAG TCTTAGTTCT AGATGGTCAC ATTGAAGATC TTCAGTGGGA CATTGATAAA
    ATTCGGAAAA GGGAACAGCG CTTAAACAGA CATTTATCTG AAGTTCTTGA GAGGGTGAAC
    TCGAAAGGTT ATAAGGTTTA TGGAGCAGGA AGCAGTCTCT ATGGGGGAAC AATCACTATA
    AATTCTCGCA AGTTTGAAGA AATGACTTCT GAAGTTGATT TGAATAAAGA GCTGGCTGTA
    AATCGACTGC AAGAGCTTGA AAAACTGAGG CAGGATTTAC AAGAAGTGAC TTCAGAAAAT
    CAGGATTTAC AGGCTGAGTT AGCCAGTGCA GTTGAAGAGA ATGTGCGGTT GTCTCCAGAG
    TATCGCTGCA TGCAGTCTCA ATTCTCTGTA TTGTACAATG AATCACTGCA GCTAAAAACT
    CAGCTAGACG AGGCTCGTTC ACTTCTACAT GGAACACGGT CCAATCATCA GCGTCAGCTA
    GAGCTTATTG AGCGTGATGA AATTTCCCTC CAGAAAAAGG TGCGCACTGA GGTTATTCAG
    TTAGAGGACA CCCTTGCTCA GGTGCGCAAG GAATATGAAA TGCTGAGGAT AGAGTTTGAG
    CAGACCCTGG CAGCCAATGA GCAAGCAGGG CCTATTAACC GAGAAATGAG ACATTTAATC
    AGCAGTCTAC AGAATCATAA TCACCAACTT AAAGGAGAGG TATTGCGGTA TAAAAGACGT
    CTGAGAGAGA TCCAAGGGGA TATAAGCAAA ATGCGTTCTC GCAGTAGCAG TAGTCTTTTC
    CTTCTGCCAT CACAGTCCAG CACTGAGGAC ACTCGTGAAG AGACAGCTGA AATTAAGACT
    GAACCTGAAG ATACAACCAC AAATGCTTCT GCCCTTTCTC AGCCAGAGGT AGTTCCAAAG
    AGGGAGGAGG AGGAGGTACA GCCACCACTG CAACAACCAC AGCGGGATAG AAGAGAGAGG
    GAAAGGGAGC GTGAAAGAGA TCGTGGAAGA GAGAAGGAGA GAGGGGATAC TTCAAAGGAG
    AAACCAAAGC ATGAGGCAGA CACTAAGCGC AAAGAGGCAG AGACGGTCAA ACAGATGAAG
    GCAGAGCTAA AGAGAGTCCA GGAATCTCTA CGAGACATGA AGCTGCTATT AGATATGTAT
    CGTTCGGCAC CAAAGGAACA AAGAGACAAA GTGCAGCTGA TGGCAGCGGA GAAAAAAAGT
    AAAGCAGAGT TGGAGGAGCT TCGTCTTCGG CTTAGAGAGC TGGAAGAAAG GGAACGCAGG
    GACAGCAAGA AGATGGCAGA CGAAGATGCC TTACGTAGAA TGAGAACAGC TGAGGAGCAG
    ACAGAGGTTC TTCAAAAAAG ACTATCAGTG GCTAAGCAGG AGGAAGAGGC CCTGCTTTCA
    GAAATGGATG TCACAGGACA GGCATTTGAA GACATGCAGG AGCAAAATAT TCGTCTTATG
    CAGCAATTGA GGGAGAAGGA TGATGCAAAC TTTAAACTTA TGTCTGAGAG AATCAAATCA
    AACCAAATCC ATAAGCTGCT CAAGGAAGAA AAGGAGGAGC TGGCAGACCA ACTTCTTACA
    CTGCGGACAC AGGTGGATGC ACAACTCCAG GTTCTAAGGA AGCTGGAAGA GAAGGAACAT
    TTACTTCAGA GTAGCATTAA TACTGGAGAG AAAGAACTTG CTCTCCGAAC CCAGGCTCTG
    GATCTGTGCA AACGTAAGAC AACAGAATCC TCTCAGCAAG CAGAAGAATT GAGAGCTCAG
    CTGGAGCTGT CTCAAAAGAA GCTGCAAGAT CTACGTGAGG AAATAATTGA GAACAGTTCA
    AGTCGAGAGA AGGATGCTTT TAATTATAAA AGAGCTCAGA AAATCTCTGC AGAGATGTAC
    ACATTTAGGA AAGGAGAAGT TGCCAAAGAC TGCTGCAGTT ACAAAGGGAA ACAGGACTTT
    TTGAGACTAT GGACAATGAA GATATCTCTC GCCTTCGAAA GAAATTGGAA TCTACAAAGA
    AACCTGATCT GGTGCCAACT TGCGATAAGA TTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951399.2
    CDS160..2955
    Translation

    Target ORF information:

    RefSeq Version XM_018095910.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger protein 20 (rnf20), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095910.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTCAGGGC CTGGAAGTAA ACGTACTGTG GGGGATGTAG GTTCTTTAGG ACCCCCTGAA 
    AAAAAGGCAG CAGTGGAAGA TTCTGGAACC ACTGTCGAGA CGATTAAACT AGGAAGTGTG
    TCTTCAACAG AGGAGCAAGA TCTGAGGACC CTTCAACTGA AAAACAAGAA GTTGGCTGGA
    ATGTTAGACC AGAGACAGGC CATAGAAGAT GAACTGCGTG ATCGTATTGA GACTTTAGAA
    AGGCGGCAAG CAACAGATGA TGCTTCTCTT CTCATCATAA ACCGATACTG GAGTCAGTTT
    GATGAAAATA TTGGCATTTT TTTGGGTCGT TATGACTTGG ATCAAGACTT GGGGGAGTTT
    CTGACAGAAC GCAAAGCATT GGTTCTGCCA GAGCCAGAGC CAGATTCTGA CAGCAATCCA
    GAACGCAAAG AAAATGAAAG GGGCGAAGGA CTATGGGAAG CTCCTCTTTC CTTTTTGGCC
    ACTCTGGCCA GCAGCAGCAG TGAAGAAATT GAATCTCAGC TACAAGAACG TGTAGAATCT
    TCACGACGTG CAGTCTCTCG TATTGTTTTG GTGTATGACC GACTGCATGA CCAACTGGAC
    CATCTTGCCA AGAAACTGAA TAGCACTGAT CCATCTCACG TGGAAGAGGC TGTCAGGGAT
    TTAAACTCCT TGCTCTCAAA TGAGAATGCA CGATTGCAGG AGATTTGTAA CTTGCTACAA
    GAGAAACAGC AGAATATGTC CCAAGAGTTC CTCCAGTTGC AGTCACGATT AGAGAGTGCT
    GAATCTCGAG TCTTAGTTCT AGATGGTCAC ATTGAAGATC TTCAGTGGGA CATTGATAAA
    ATTCGGAAAA GGGAACAGCG CTTAAACAGA CATTTATCTG AAGTTCTTGA GAGGGTGAAC
    TCGAAAGGTT ATAAGGTTTA TGGAGCAGGA AGCAGTCTCT ATGGGGGAAC AATCACTATA
    AATTCTCGCA AGTTTGAAGA AATGACTTCT GAAGTTGATT TGAATAAAGA GCTGGCTGTA
    AATCGACTGC AAGAGCTTGA AAAACTGAGG CAGGATTTAC AAGAAGTGAC TTCAGAAAAT
    CAGGATTTAC AGGCTGAGTT AGCCAGTGCA GTTGAAGAGA ATGTGCGGTT GTCTCCAGAG
    TATCGCTGCA TGCAGTCTCA ATTCTCTGTA TTGTACAATG AATCACTGCA GCTAAAAACT
    CAGCTAGACG AGGCTCGTTC ACTTCTACAT GGAACACGGT CCAATCATCA GCGTCAGCTA
    GAGCTTATTG AGCGTGATGA AATTTCCCTC CAGAAAAAGG TGCGCACTGA GGTTATTCAG
    TTAGAGGACA CCCTTGCTCA GGTGCGCAAG GAATATGAAA TGCTGAGGAT AGAGTTTGAG
    CAGACCCTGG CAGCCAATGA GCAAGCAGGG CCTATTAACC GAGAAATGAG ACATTTAATC
    AGCAGTCTAC AGAATCATAA TCACCAACTT AAAGGAGAGG TATTGCGGTA TAAAAGACGT
    CTGAGAGAGA TCCAAGGGGA TATAAGCAAA ATGCGTTCTC GCAGTAGCAG TAGTCTTTTC
    CTTCTGCCAT CACAGTCCAG CACTGAGGAC ACTCGTGAAG AGACAGCTGA AATTAAGACT
    GAACCTGAAG ATACAACCAC AAATGCTTCT GCCCTTTCTC AGCCAGAGGT AGTTCCAAAG
    AGGGAGGAGG AGGAGGTACA GCCACCACTG CAACAACCAC AGCGGGATAG AAGAGAGAGG
    GAAAGGGAGC GTGAAAGAGA TCGTGGAAGA GAGAAGGAGA GAGGGGATAC TTCAAAGGAG
    AAACCAAAGC ATGAGGCAGA CACTAAGCGC AAAGAGGCAG AGACGGTCAA ACAGATGAAG
    GCAGAGCTAA AGAGAGTCCA GGAATCTCTA CGAGACATGA AGCTGCTATT AGATATGTAT
    CGTTCGGCAC CAAAGGAACA AAGAGACAAA GTGCAGCTGA TGGCAGCGGA GAAAAAAAGT
    AAAGCAGAGT TGGAGGAGCT TCGTCTTCGG CTTAGAGAGC TGGAAGAAAG GGAACGCAGG
    GACAGCAAGA AGATGGCAGA CGAAGATGCC TTACGTAGAA TGAGAACAGC TGAGGAGCAG
    ACAGAGGTTC TTCAAAAAAG ACTATCAGTG GCTAAGCAGG AGGAAGAGGC CCTGCTTTCA
    GAAATGGATG TCACAGGACA GGCATTTGAA GACATGCAGG AGCAAAATAT TCGTCTTATG
    CAGCAATTGA GGGAGAAGGA TGATGCAAAC TTTAAACTTA TGTCTGAGAG AATCAAATCA
    AACCAAATCC ATAAGCTGCT CAAGGAAGAA AAGGAGGAGC TGGCAGACCA ACTTCTTACA
    CTGCGGACAC AGGTGGATGC ACAACTCCAG GTTCTAAGGA AGCTGGAAGA GAAGGAACAT
    TTACTTCAGA GTAGCATTAA TACTGGAGAG AAAGAACTTG CTCTCCGAAC CCAGGCTCTG
    GATCTGTGCA AACGTAAGAC AACAGAATCC TCTCAGCAAG CAGAAGAATT GAGAGCTCAG
    CTGGAGCTGT CTCAAAAGAA GCTGCAAGAT CTACGTGAGG AAATAATTGA GAACAGTTCA
    AGTCGAGAGA AGGATGCTTT TAATTATAAA AGAGCTCAGA AAATCTCTGC AGAGATGTAC
    ACATTTAGGA AAGGAGAAGT TGCCAAAGAC TGCTGCAGTT ACAAAGGGAA ACAGGACTTT
    TTGAGACTAT GGACAATGAA GATATCTCTC GCCTTCGAAA GAAATTGGAA TCTACAAAGA
    AACCTGATCT GGTGCCAACT TGCGATAAGA TTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.