arhgap32 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012967128.1	XP_012822582.1	rho GTPase-activating protein 32 isoform X7
XM_018095928.2	XP_017951417.2	rho GTPase-activating protein 32 isoform X2
XM_018095931.2	XP_017951420.2	rho GTPase-activating protein 32 isoform X7
XM_004916166.3	XP_004916223.1	rho GTPase-activating protein 32 isoform X3
XM_004916168.3	XP_004916225.1	rho GTPase-activating protein 32 isoform X6
XM_004916165.4	XP_004916222.2	rho GTPase-activating protein 32 isoform X1
XM_004916165.3	XP_004916222.1	rho GTPase-activating protein 32 isoform X1
XM_004916169.3	XP_004916226.1	rho GTPase-activating protein 32 isoform X9
XM_018095931.1	XP_017951420.1	rho GTPase-activating protein 32 isoform X8
XM_004916164.4	XP_004916221.2	rho GTPase-activating protein 32 isoform X1
XM_031905577.1	XP_031761437.1	rho GTPase-activating protein 32 isoform X5
XM_012967128.2	XP_012822582.2	rho GTPase-activating protein 32 isoform X6
XM_004916170.4	XP_004916227.2	rho GTPase-activating protein 32 isoform X8
XM_004916164.3	XP_004916221.1	rho GTPase-activating protein 32 isoform X1
XM_018095928.1	XP_017951417.1	rho GTPase-activating protein 32 isoform X2
XM_018095929.1	XP_017951418.1	rho GTPase-activating protein 32 isoform X4
XM_018095930.1	XP_017951419.1	rho GTPase-activating protein 32 isoform X5
XM_004916170.3	XP_004916227.1	rho GTPase-activating protein 32 isoform X9
XM_031905576.1	XP_031761436.1	rho GTPase-activating protein 32 isoform X1
XM_004916166.4	XP_004916223.2	rho GTPase-activating protein 32 isoform X3
XM_018095929.2	XP_017951418.2	rho GTPase-activating protein 32 isoform X4
XM_004916168.4	XP_004916225.2	rho GTPase-activating protein 32 isoform X5
XM_004916169.4	XP_004916226.2	rho GTPase-activating protein 32 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa45813	XM_018095928.1 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa45814	XM_018095929.1 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa45815	XM_018095931.1 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa61961	XM_004916165.4 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa61961	XM_004916164.4 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa61961	XM_031905576.1 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa61962	XM_018095928.2 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa61963	XM_004916166.4 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa61964	XM_018095929.2 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa61965	XM_004916168.4 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa61965	XM_031905577.1 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa61966	XM_012967128.2 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa61967	XM_018095931.2 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa61968	XM_004916170.4 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa61968	XM_004916169.4 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa13195	XM_004916165.3 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa13195	XM_004916164.3 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa13196	XM_004916166.3 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa13197	XM_018095930.1 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa13198	XM_004916168.3 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa13199	XM_004916170.3 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa13199	XM_004916169.3 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa28809	XM_012967128.1 Latest version!	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

arhgap32 ( XM_018095928.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951417.1 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa45813
Clone ID Related Accession (Same CDS sequence)	XM_018095928.1
Accession Version	XM_018095928.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGATTTG 
GAGATGGATA GTTTAAGGCC TGGAACATCG TGCTGTTCTG AGGTATTAAG TCACTCGCAG 
CTAAGCTCAG TGTCCCGGAA GCACGGCAAG CTCATCACAT TTCTAAGAAC CTTCATGAAG 
TCCAGACCCA GCAAACAGAA ACTGAAGCAG AGAGGGATCC TGAGGGAGCG AGTGTTTGGT 
TGTGACCTTG GGGAACATCT GCTTAACTCT GGTCAGGATG TCCCCCAAGT CCTCAGGAGC 
TGCACAGAGT TTATAGAAAA ACATGGCATT GTTGATGGAA TCTACCGTCT GTCTGGGATT 
GCATCTAACA TCCAGAAACT ACGTCATGAA TTTGACTCTG AACAGATTCC AGACCTGACT 
AAAGATGTGT ACATCCAGGA CATTCACTGT GTTGGCTCTC TCTGTAAACT CTACTTCCGG 
GAACTCCCAA ACCCTCTGCT CACATATCAG TTGTATGAAA AGTTCTCTGA TGCTGTGTCA 
GCGGCTACTG ATGAAGAGAG ACTGGTGAAA ATTCATGATG TTATTCAACA GCTTCCCCCA 
CCTCATTACA GAACACTGGA GTTCCTTATG CGCCACTTAT CAAGACTTGC GACATACTGC 
TCTATCACCA ATATGCACAC TAAAAATTTG GCAATTGTCT GGGCCCCTAA CCTGCTCCGG 
TCAAAACAGA TTGAGTCTGC ATGTTTTAGC GGTACAGCAG CTTTCATGGA AGTGCGCATA 
CAGTCTGTGG TAGTTGAATT TATTCTAAAC CATGTGGAGG TTCTCTTTAG TCCTAAACTT 
AGTTCTGTGA TAAGGGATGG TGCTGGTCAC ACTTCTTTGT CTCGGCCCAA GTCACTGTTG 
GTGTCATCTC CATCTACAAA ACTACTAAGC CTAGAGGAGG CTCAAGCAAG GACACAGGCC 
CAAATCAATT CCCCTGTTGT GGCTGACAGC AAGTACATTG AGGTGGGAGA GGGACCAGCT 
GCTTTGCAGG GCAGATTCCA CACTGTAATT GACTTCCCTT CTGAAAGGAA AAGACCGCCA 
ACCAAAATGA AAAAATCTCC TGTTGGAAGC TGGCGTTCTT TTTTTAGCAT AGGCAAATCA 
TCATCTTCAG CCTCCATGTC TAAGCGGAAA CTTCAGCGTA ATCCAAGTGA GCCTTTGGAA 
ATGAAATCCA TGGCATTTGC AGGTGGAAGA GATAGCAGCA CCATGCGCTC AGCTAAAAGC 
GAGGAGTCTC TCAGTTCCTT GCATGCAGCA GATGGAGAGT CGAAGCTATT TCGTCCACGT 
CGTCCTCGTT CCAGCAGCGA TGCTCTGTCT GCTTCATATA ATGGGGACCT TCTGGATAGC 
TGCAACCGTT GCAACTCCTA TGATAATCTG CCCCGAGACC AAGACAGTGA TGGAGATGAG 
GGTTTGATTC ATGTGCCTGC TATGCTGTCA GGTCGCTCTC CTGAGGATGA AGAACTCAGC 
CCACCAGACA TTGGTATGGC CAGTTTGGAC TTTGACCCAA TGTCTTTTCA GTGCAGCCCT 
CCCCAGCTAG AATCTGAAGG ACCAGACATT TTTTTTCAGC TTGACATTTG TGCTAGCAAT 
GGCAAGGATA AGGTGCAAAG TGGTGCACAA AACCTAGGCG GTGTCTCCAG CTCCGAACCT 
CACTCTCCTT TCCAAGAGAA AGTCGTTAGT CCATTTCTAT CGCCTGACCG CAGTTCTAGT 
ACAGATAAAG TATCCAAAAC TACATCATTT GCAGAAAAAG TTGTGCAGGC CTTGTCACCT 
AAAGTGGGGC GCAAAGCAGT TCGATCTCCT CCATTAACCA TCTCAGAGCC TGTCACCATC 
TCTCTCCCTA GTCGGGTATC GGAAATGATT GGAAGTATGC AAGGCAATAC TGCTGCTTCC 
CAGTCTTTAA TTTGGTACAG AGAAAACAGT TTAAATTCTA GAGAAACTGA AATGAATTAT 
GTTTGTAAAT CGGTGGATGC CAACCTTCCA TCCTTATTGC CAGCAACAGA TCCATGGTCT 
ACAGTTACTC CTGTAATTAC TCAATGGAAC CCAGATGGAT TACAGAGAGA AGACAAAGCA 
GGACCATTTA CTCATGGCTT TCCCCAGCCA AATAGTGACT TGGAACAAGT AGAAAACTTC 
AACCTGGAAC TTAGCACAAC AAATGTTGCT GCAGCATATC AGAAAGAAGA TACAGAATTG 
TCGAGAGCAA ATCAGAACAA ATCCCACTCT TCTGTTTCTC CCCCTCAAGG AGCTTCTGCT 
TCAGAATCTC CACAGGAGCT AGCCCCTGTC AGTAGTGTTT CTATCATTCC ACCTCCACCT 
CCTCCAAAGA ATCCTGCACG CATGCTGGCA TTAGCACTGG CTGAATCTGC TCAACAGGCA 
TCTGCTAATA AAAGACCTCT TTATATGCAG TTCATGGATC TACCCCCCCA TCCTCCTCCA 
CCATCAGTAC TAGAGGACAA ACCACTTCTT GAGTTATCTC CAAAAATGTC TCCAGAGCAC 
CTCGTATCTG GTCCTGCAAC ACCAGCGACC AGTCCTCCAG TCATATCCAA TAAAACATCT 
CCAGCTACTC CACCAAGCAC AACTTCATCA TTTTCTATCA CAACAATTCA TCACAGTCCT 
GTGAAAAACC ATAGTCCTCT GGCAGGACAG ATGCCTACAG CAGTCACTCC TGGACTCAGT 
CCTTCATCAA ACCAGGTTCC TGTATCCTTT CTATCTGATG AAATGCTACC TTCTGAAACA 
AATAAAATGA ATTCTGTCAT ACTGAGTGAA GCATTTACAA CAGGAATTCC TGTTACTCCA 
CCATCTGAAA AACCCAAGGA AATTTCTAGA CCACCACATC TTCACCAGCG TTCTGAATCC 
TTTCCTTCAC ATCCTGCATA TAGCAATGCA AAGCCCACTC CACCAGTTAG AACAATGGAT 
AGTCGACTGG CAACTGCCAT GCATTCCAAC TTCAATGATG CCATCACTGC TAATAACTAC 
CACTCCTTCC TGAACACCAT GATGCTTCCT TCTTCTCTAG AGGATGTGCT ACCCAGACAC 
AACTATTCTC ATTCTTCTCA TCTCAAGACA GAAAATATAG ATGAACAGGG AGTTAATTAC 
AGACAACCTT ATTTATCCAA CACCAAGCAA GAGGACCCAG CTGATGTGGG AGATTTATAC 
AGACAGCAAT ATATTTCTAC TGGAAAGTCT GAGAATGCTG AGATAGGAAA CACTTACCGG 
CACCCATATC CTTCCAATAC TGAGCCAGAA AGTCTAAAGG AGCCACTAGA TCCTTTTCTG 
CATCACAAGT CAGCAGTTGC TCCAAAGACA TATAGAGCAG AAACACTTCT ACCACATATG 
CCTCCTCAAG TTTATGGTTC AAGATGTGAT ACTCCATCCT CAACATTTTA TGGCACCTAC 
ATAAATCAGG CCAAACACCC CCGCACCAGG AACAGACCTG ATTATATGTC CTCTATGAGC 
CCAGGTCTCC GTAGCTACTC AGAAGACACA TCTTCCTACC CTACTATTCG AAGGGTACAA 
TCTCTGCATG TGCCTATGCA ACCTCCGCCA CCTCCTATCC GCACTGTTCC AATTTCAAGA 
ACAGAGGTTC CCCCAGATGA TGATCCAGCA TACTGCCCAA GACCAGTTTA TCAATACAAA 
CAATGCCCCC AATTTAACTC TCAGTCAGAT TACCATGTTA CCCAGCTGCA GCCTTACTTT 
GAAAATGGAC GAGTTCATTA TCGCTACAGT CCCTATTCAG GGGGCCCAAG TTATTTTTCA 
GCAGATAGCA ATTTCTATGA TATGGACCCC TACAGCACCT TACGTCTGCG ACAACTTCCT 
CTCTACCCAA GCCGGGACTT TGCTTCTTAC ACCACAAGAT TGCAGCCCAA AAACACATAT 
CGGTCACAAG GATTGCCCCC ATATCCCAGA GGAACTCTAC GGGAACACAA TTTTATCAGT 
AGAGATGTTC CTCCTGCACT ACCACCTGAA CATCCACGAC CTCTTCACAT TTCTTGGGAC 
ATGGAAGACA TGGACAGATA CAGACTGCAA TCCCTGCGAC GAGAGAACAG GGCACGTCAG 
AGAGCTAAAG GACCAGTAAT GTCTCAGTAT GATAATGTTA CACCAACCTT AGTGGAGGAT 
GTAGCAGGCT TAGATGTCAT ACATCTCCGA AGCCGTTCTG ATCCTGGTAA GACTCCTGGT 
CTTTTAAGTG TGGCTGAGAA CAAAGACGTC CGTTACCCAG GAAGGGCAGA AGGTGATGAG 
CATACAACAT ATCCACCTCC TGTTTTTGGA AATGGCCATC AAGATAAGCC ATCTCTTCCT 
CAAAAGCAAA GTGGATCAAG CCGGGTTCGA ATGCAACATG AAATATCTAC TGAGCAGCGG 
ACTCAACCTT CTCAAGATAC TTTGCATAGG CAACCCACAG AGGGACGAAA TGGTCCTCCA 
ATTCCTCCTG TGGACTACAG CTCTAAAGGA ATGCCAAATC CCCCAGACCC TGCATCATAT 
CATTCCTCTG CAAACAAATA TAACTTGACA CAACAGGAAC CCATGCGACT TATTCACAAA 
GAGCTTCGGC TCCCTGAGGA TATTGAGCAT TCACATACCA GGCCAGCTGA CAACCAACAC 
CGTGACTGTT ACAGGGAGGA GCCAGCTCAG TTTCCCCTTA CTGCACCTCC TCCTAAACCA 
GAGAGGAGCC ATAGCCTTCG AGGTCATAAT CCAGATGTTT TAGAAAGAGA CCCTAGTATC 
TTCTACCCCT ACCAAACGCT TCAAGCCAAG AGACACAGCA CAATTAATGC AGTGTCACAA 
TATGATAATC TAGCTGATTA CCATTCTATA CCCCATCATC GAACCACTAA CCAGTCTACT 
CCAAATGCCT TTAACCTCCC ACATGGACGG ACCTATGCTA CTGCCCTGGG CCAGGGTGCC 
TTTCTAGCTG CTGAACTCGG CTTGCAAAGG CCTGAGACAA AAATCCATGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951417.1
CDS	3018..8954
Translation	MEGGTDAAAFAAFPSIHAVPSTSGRMATGGKLDELERIMKSSLHLEEDDFVPELPRSIHPRERPDWEETISAMARGADIPVPGEIPTMPTEIPLRSCGSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGSIQLTLTEDQNEVSRNGCESKELVFLVQIACQGRSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLSELPRSDSVKDSPELVTQMLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINVPAIAAAHVIKRYNAQAPDELSFEVGDIVSVIDMPPKELTTWWRGKHGFQVGFFPSECVELINDKVPQSMTNSVPKPDLEMDSLRPGTSCCSEVLSHSQLSSVSRKHGKLITFLRTFMKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSGAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_018095928.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095928.1

ORF Insert Sequence:

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGATTTG 
GAGATGGATA GTTTAAGGCC TGGAACATCG TGCTGTTCTG AGGTATTAAG TCACTCGCAG 
CTAAGCTCAG TGTCCCGGAA GCACGGCAAG CTCATCACAT TTCTAAGAAC CTTCATGAAG 
TCCAGACCCA GCAAACAGAA ACTGAAGCAG AGAGGGATCC TGAGGGAGCG AGTGTTTGGT 
TGTGACCTTG GGGAACATCT GCTTAACTCT GGTCAGGATG TCCCCCAAGT CCTCAGGAGC 
TGCACAGAGT TTATAGAAAA ACATGGCATT GTTGATGGAA TCTACCGTCT GTCTGGGATT 
GCATCTAACA TCCAGAAACT ACGTCATGAA TTTGACTCTG AACAGATTCC AGACCTGACT 
AAAGATGTGT ACATCCAGGA CATTCACTGT GTTGGCTCTC TCTGTAAACT CTACTTCCGG 
GAACTCCCAA ACCCTCTGCT CACATATCAG TTGTATGAAA AGTTCTCTGA TGCTGTGTCA 
GCGGCTACTG ATGAAGAGAG ACTGGTGAAA ATTCATGATG TTATTCAACA GCTTCCCCCA 
CCTCATTACA GAACACTGGA GTTCCTTATG CGCCACTTAT CAAGACTTGC GACATACTGC 
TCTATCACCA ATATGCACAC TAAAAATTTG GCAATTGTCT GGGCCCCTAA CCTGCTCCGG 
TCAAAACAGA TTGAGTCTGC ATGTTTTAGC GGTACAGCAG CTTTCATGGA AGTGCGCATA 
CAGTCTGTGG TAGTTGAATT TATTCTAAAC CATGTGGAGG TTCTCTTTAG TCCTAAACTT 
AGTTCTGTGA TAAGGGATGG TGCTGGTCAC ACTTCTTTGT CTCGGCCCAA GTCACTGTTG 
GTGTCATCTC CATCTACAAA ACTACTAAGC CTAGAGGAGG CTCAAGCAAG GACACAGGCC 
CAAATCAATT CCCCTGTTGT GGCTGACAGC AAGTACATTG AGGTGGGAGA GGGACCAGCT 
GCTTTGCAGG GCAGATTCCA CACTGTAATT GACTTCCCTT CTGAAAGGAA AAGACCGCCA 
ACCAAAATGA AAAAATCTCC TGTTGGAAGC TGGCGTTCTT TTTTTAGCAT AGGCAAATCA 
TCATCTTCAG CCTCCATGTC TAAGCGGAAA CTTCAGCGTA ATCCAAGTGA GCCTTTGGAA 
ATGAAATCCA TGGCATTTGC AGGTGGAAGA GATAGCAGCA CCATGCGCTC AGCTAAAAGC 
GAGGAGTCTC TCAGTTCCTT GCATGCAGCA GATGGAGAGT CGAAGCTATT TCGTCCACGT 
CGTCCTCGTT CCAGCAGCGA TGCTCTGTCT GCTTCATATA ATGGGGACCT TCTGGATAGC 
TGCAACCGTT GCAACTCCTA TGATAATCTG CCCCGAGACC AAGACAGTGA TGGAGATGAG 
GGTTTGATTC ATGTGCCTGC TATGCTGTCA GGTCGCTCTC CTGAGGATGA AGAACTCAGC 
CCACCAGACA TTGGTATGGC CAGTTTGGAC TTTGACCCAA TGTCTTTTCA GTGCAGCCCT 
CCCCAGCTAG AATCTGAAGG ACCAGACATT TTTTTTCAGC TTGACATTTG TGCTAGCAAT 
GGCAAGGATA AGGTGCAAAG TGGTGCACAA AACCTAGGCG GTGTCTCCAG CTCCGAACCT 
CACTCTCCTT TCCAAGAGAA AGTCGTTAGT CCATTTCTAT CGCCTGACCG CAGTTCTAGT 
ACAGATAAAG TATCCAAAAC TACATCATTT GCAGAAAAAG TTGTGCAGGC CTTGTCACCT 
AAAGTGGGGC GCAAAGCAGT TCGATCTCCT CCATTAACCA TCTCAGAGCC TGTCACCATC 
TCTCTCCCTA GTCGGGTATC GGAAATGATT GGAAGTATGC AAGGCAATAC TGCTGCTTCC 
CAGTCTTTAA TTTGGTACAG AGAAAACAGT TTAAATTCTA GAGAAACTGA AATGAATTAT 
GTTTGTAAAT CGGTGGATGC CAACCTTCCA TCCTTATTGC CAGCAACAGA TCCATGGTCT 
ACAGTTACTC CTGTAATTAC TCAATGGAAC CCAGATGGAT TACAGAGAGA AGACAAAGCA 
GGACCATTTA CTCATGGCTT TCCCCAGCCA AATAGTGACT TGGAACAAGT AGAAAACTTC 
AACCTGGAAC TTAGCACAAC AAATGTTGCT GCAGCATATC AGAAAGAAGA TACAGAATTG 
TCGAGAGCAA ATCAGAACAA ATCCCACTCT TCTGTTTCTC CCCCTCAAGG AGCTTCTGCT 
TCAGAATCTC CACAGGAGCT AGCCCCTGTC AGTAGTGTTT CTATCATTCC ACCTCCACCT 
CCTCCAAAGA ATCCTGCACG CATGCTGGCA TTAGCACTGG CTGAATCTGC TCAACAGGCA 
TCTGCTAATA AAAGACCTCT TTATATGCAG TTCATGGATC TACCCCCCCA TCCTCCTCCA 
CCATCAGTAC TAGAGGACAA ACCACTTCTT GAGTTATCTC CAAAAATGTC TCCAGAGCAC 
CTCGTATCTG GTCCTGCAAC ACCAGCGACC AGTCCTCCAG TCATATCCAA TAAAACATCT 
CCAGCTACTC CACCAAGCAC AACTTCATCA TTTTCTATCA CAACAATTCA TCACAGTCCT 
GTGAAAAACC ATAGTCCTCT GGCAGGACAG ATGCCTACAG CAGTCACTCC TGGACTCAGT 
CCTTCATCAA ACCAGGTTCC TGTATCCTTT CTATCTGATG AAATGCTACC TTCTGAAACA 
AATAAAATGA ATTCTGTCAT ACTGAGTGAA GCATTTACAA CAGGAATTCC TGTTACTCCA 
CCATCTGAAA AACCCAAGGA AATTTCTAGA CCACCACATC TTCACCAGCG TTCTGAATCC 
TTTCCTTCAC ATCCTGCATA TAGCAATGCA AAGCCCACTC CACCAGTTAG AACAATGGAT 
AGTCGACTGG CAACTGCCAT GCATTCCAAC TTCAATGATG CCATCACTGC TAATAACTAC 
CACTCCTTCC TGAACACCAT GATGCTTCCT TCTTCTCTAG AGGATGTGCT ACCCAGACAC 
AACTATTCTC ATTCTTCTCA TCTCAAGACA GAAAATATAG ATGAACAGGG AGTTAATTAC 
AGACAACCTT ATTTATCCAA CACCAAGCAA GAGGACCCAG CTGATGTGGG AGATTTATAC 
AGACAGCAAT ATATTTCTAC TGGAAAGTCT GAGAATGCTG AGATAGGAAA CACTTACCGG 
CACCCATATC CTTCCAATAC TGAGCCAGAA AGTCTAAAGG AGCCACTAGA TCCTTTTCTG 
CATCACAAGT CAGCAGTTGC TCCAAAGACA TATAGAGCAG AAACACTTCT ACCACATATG 
CCTCCTCAAG TTTATGGTTC AAGATGTGAT ACTCCATCCT CAACATTTTA TGGCACCTAC 
ATAAATCAGG CCAAACACCC CCGCACCAGG AACAGACCTG ATTATATGTC CTCTATGAGC 
CCAGGTCTCC GTAGCTACTC AGAAGACACA TCTTCCTACC CTACTATTCG AAGGGTACAA 
TCTCTGCATG TGCCTATGCA ACCTCCGCCA CCTCCTATCC GCACTGTTCC AATTTCAAGA 
ACAGAGGTTC CCCCAGATGA TGATCCAGCA TACTGCCCAA GACCAGTTTA TCAATACAAA 
CAATGCCCCC AATTTAACTC TCAGTCAGAT TACCATGTTA CCCAGCTGCA GCCTTACTTT 
GAAAATGGAC GAGTTCATTA TCGCTACAGT CCCTATTCAG GGGGCCCAAG TTATTTTTCA 
GCAGATAGCA ATTTCTATGA TATGGACCCC TACAGCACCT TACGTCTGCG ACAACTTCCT 
CTCTACCCAA GCCGGGACTT TGCTTCTTAC ACCACAAGAT TGCAGCCCAA AAACACATAT 
CGGTCACAAG GATTGCCCCC ATATCCCAGA GGAACTCTAC GGGAACACAA TTTTATCAGT 
AGAGATGTTC CTCCTGCACT ACCACCTGAA CATCCACGAC CTCTTCACAT TTCTTGGGAC 
ATGGAAGACA TGGACAGATA CAGACTGCAA TCCCTGCGAC GAGAGAACAG GGCACGTCAG 
AGAGCTAAAG GACCAGTAAT GTCTCAGTAT GATAATGTTA CACCAACCTT AGTGGAGGAT 
GTAGCAGGCT TAGATGTCAT ACATCTCCGA AGCCGTTCTG ATCCTGGTAA GACTCCTGGT 
CTTTTAAGTG TGGCTGAGAA CAAAGACGTC CGTTACCCAG GAAGGGCAGA AGGTGATGAG 
CATACAACAT ATCCACCTCC TGTTTTTGGA AATGGCCATC AAGATAAGCC ATCTCTTCCT 
CAAAAGCAAA GTGGATCAAG CCGGGTTCGA ATGCAACATG AAATATCTAC TGAGCAGCGG 
ACTCAACCTT CTCAAGATAC TTTGCATAGG CAACCCACAG AGGGACGAAA TGGTCCTCCA 
ATTCCTCCTG TGGACTACAG CTCTAAAGGA ATGCCAAATC CCCCAGACCC TGCATCATAT 
CATTCCTCTG CAAACAAATA TAACTTGACA CAACAGGAAC CCATGCGACT TATTCACAAA 
GAGCTTCGGC TCCCTGAGGA TATTGAGCAT TCACATACCA GGCCAGCTGA CAACCAACAC 
CGTGACTGTT ACAGGGAGGA GCCAGCTCAG TTTCCCCTTA CTGCACCTCC TCCTAAACCA 
GAGAGGAGCC ATAGCCTTCG AGGTCATAAT CCAGATGTTT TAGAAAGAGA CCCTAGTATC 
TTCTACCCCT ACCAAACGCT TCAAGCCAAG AGACACAGCA CAATTAATGC AGTGTCACAA 
TATGATAATC TAGCTGATTA CCATTCTATA CCCCATCATC GAACCACTAA CCAGTCTACT 
CCAAATGCCT TTAACCTCCC ACATGGACGG ACCTATGCTA CTGCCCTGGG CCAGGGTGCC 
TTTCTAGCTG CTGAACTCGG CTTGCAAAGG CCTGAGACAA AAATCCATGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_018095929.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951418.1 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X5, mRNA.

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CloneID	OXa45814
Clone ID Related Accession (Same CDS sequence)	XM_018095929.1
Accession Version	XM_018095929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGAA TAATGAAGTC TTCACTGCAC TTAGAGGAGG ATGATTTTGT ACCCGAGTTG 
CCAAGGAGTA TCCATCCCCG GGAGAGGCCG GACTGGGAGG AGACTATTAG TGCCATGGCA 
CGAGGGGCAG ACATCCCAGT CCCAGGGGAA ATACCGACGA TGCCTACAGA AATCCCTCTT 
CGCTCCTGTG GCAGTACTGC CAGCATGAAG GTGAAAAATG TCAAGAAGTT ATCCTTCACG 
AAAGGGCATT TTCCTAAACT TGCAGAATGT GCACATTTCC ACTATGAAAA TGTAGACTTT 
GGCAGCATAC AGCTCACACT CACAGAAGAT CAAAATGAAG TGAGCAGAAA TGGCTGTGAA 
TCCAAGGAAC TTGTTTTCCT AGTACAGATT GCTTGTCAGG GCCGTAGCTG GATAGTTAAG 
CGGAGTTATG AAGATTTTCG GGTTCTGGAT AAACACCTTC ATCTGTGCAT ATATGATAGG 
CGCTTCTCTC AGCTCTCAGA GCTGCCCCGT TCCGACTCCG TAAAGGATAG TCCAGAGTTG 
GTCACACAGA TGCTGATGGC TTACCTATCG CGGCTGTCAG CCATCGCAGG TAACAAGATC 
AATTGTGGGC CAGCTCTGAC GTGGATGGAG ATCGATAATA AAGGCAATCA TTTGCTTGTG 
CACGAAGAGT CCTCCATAAA TGTTCCAGCC ATCGCTGCTG CCCATGTCAT AAAGCGATAT 
AATGCTCAGG CCCCAGATGA ACTCTCTTTT GAGGTGGGAG ACATAGTATC TGTAATTGAC 
ATGCCACCAA AAGAACTGAC CACGTGGTGG AGAGGAAAGC ATGGATTTCA AGTGGGTTTC 
TTTCCAAGTG AATGTGTGGA ATTAATAAAT GATAAAGTCC CCCAATCAAT GACAAACTCT 
GTGCCTAAAC CAGTCTCCCC TGCGCATGGA ATGAGGCCGG CCTCCTGGAG CTACTTTCCC 
CCCTATTTGG AGATGGATAG TTTAAGGCCT GGAACATCGT GCTGTTCTGA GGTATTAAGT 
CACTCGCAGC TAAGCTCAGT GTCCCGGAAG CACGGCAAGC TCATCACATT TCTAAGAACC 
TTCATGAAGT CCAGACCCAG CAAACAGAAA CTGAAGCAGA GAGGGATCCT GAGGGAGCGA 
GTGTTTGGTT GTGACCTTGG GGAACATCTG CTTAACTCTG GTCAGGATGT CCCCCAAGTC 
CTCAGGAGCT GCACAGAGTT TATAGAAAAA CATGGCATTG TTGATGGAAT CTACCGTCTG 
TCTGGGATTG CATCTAACAT CCAGAAACTA CGTCATGAAT TTGACTCTGA ACAGATTCCA 
GACCTGACTA AAGATGTGTA CATCCAGGAC ATTCACTGTG TTGGCTCTCT CTGTAAACTC 
TACTTCCGGG AACTCCCAAA CCCTCTGCTC ACATATCAGT TGTATGAAAA GTTCTCTGAT 
GCTGTGTCAG CGGCTACTGA TGAAGAGAGA CTGGTGAAAA TTCATGATGT TATTCAACAG 
CTTCCCCCAC CTCATTACAG AACACTGGAG TTCCTTATGC GCCACTTATC AAGACTTGCG 
ACATACTGCT CTATCACCAA TATGCACACT AAAAATTTGG CAATTGTCTG GGCCCCTAAC 
CTGCTCCGGT CAAAACAGAT TGAGTCTGCA TGTTTTAGCG GTACAGCAGC TTTCATGGAA 
GTGCGCATAC AGTCTGTGGT AGTTGAATTT ATTCTAAACC ATGTGGAGGT TCTCTTTAGT 
CCTAAACTTA GTTCTGTGAT AAGGGATGGT GCTGGTCACA CTTCTTTGTC TCGGCCCAAG 
TCACTGTTGG TGTCATCTCC ATCTACAAAA CTACTAAGCC TAGAGGAGGC TCAAGCAAGG 
ACACAGGCCC AAATCAATTC CCCTGTTGTG GCTGACAGCA AGTACATTGA GGTGGGAGAG 
GGACCAGCTG CTTTGCAGGG CAGATTCCAC ACTGTAATTG ACTTCCCTTC TGAAAGGAAA 
AGACCGCCAA CCAAAATGAA AAAATCTCCT GTTGGAAGCT GGCGTTCTTT TTTTAGCATA 
GGCAAATCAT CATCTTCAGC CTCCATGTCT AAGCGGAAAC TTCAGCGTAA TCCAAGTGAG 
CCTTTGGAAA TGAAATCCAT GGCATTTGCA GGTGGAAGAG ATAGCAGCAC CATGCGCTCA 
GCTAAAAGCG AGGAGTCTCT CAGTTCCTTG CATGCAGCAG ATGGAGAGTC GAAGCTATTT 
CGTCCACGTC GTCCTCGTTC CAGCAGCGAT GCTCTGTCTG CTTCATATAA TGGGGACCTT 
CTGGATAGCT GCAACCGTTG CAACTCCTAT GATAATCTGC CCCGAGACCA AGACAGTGAT 
GGAGATGAGG GTTTGATTCA TGTGCCTGCT ATGCTGTCAG GTCGCTCTCC TGAGGATGAA 
GAACTCAGCC CACCAGACAT TGGTATGGCC AGTTTGGACT TTGACCCAAT GTCTTTTCAG 
TGCAGCCCTC CCCAGCTAGA ATCTGAAGGA CCAGACATTT TTTTTCAGCT TGACATTTGT 
GCTAGCAATG GCAAGGATAA GGTGCAAAGT GGTGCACAAA ACCTAGGCGG TGTCTCCAGC 
TCCGAACCTC ACTCTCCTTT CCAAGAGAAA GTCGTTAGTC CATTTCTATC GCCTGACCGC 
AGTTCTAGTA CAGATAAAGT ATCCAAAACT ACATCATTTG CAGAAAAAGT TGTGCAGGCC 
TTGTCACCTA AAGTGGGGCG CAAAGCAGTT CGATCTCCTC CATTAACCAT CTCAGAGCCT 
GTCACCATCT CTCTCCCTAG TCGGGTATCG GAAATGATTG GAAGTATGCA AGGCAATACT 
GCTGCTTCCC AGTCTTTAAT TTGGTACAGA GAAAACAGTT TAAATTCTAG AGAAACTGAA 
ATGAATTATG TTTGTAAATC GGTGGATGCC AACCTTCCAT CCTTATTGCC AGCAACAGAT 
CCATGGTCTA CAGTTACTCC TGTAATTACT CAATGGAACC CAGATGGATT ACAGAGAGAA 
GACAAAGCAG GACCATTTAC TCATGGCTTT CCCCAGCCAA ATAGTGACTT GGAACAAGTA 
GAAAACTTCA ACCTGGAACT TAGCACAACA AATGTTGCTG CAGCATATCA GAAAGAAGAT 
ACAGAATTGT CGAGAGCAAA TCAGAACAAA TCCCACTCTT CTGTTTCTCC CCCTCAAGGA 
GCTTCTGCTT CAGAATCTCC ACAGGAGCTA GCCCCTGTCA GTAGTGTTTC TATCATTCCA 
CCTCCACCTC CTCCAAAGAA TCCTGCACGC ATGCTGGCAT TAGCACTGGC TGAATCTGCT 
CAACAGGCAT CTGCTAATAA AAGACCTCTT TATATGCAGT TCATGGATCT ACCCCCCCAT 
CCTCCTCCAC CATCAGTACT AGAGGACAAA CCACTTCTTG AGTTATCTCC AAAAATGTCT 
CCAGAGCACC TCGTATCTGG TCCTGCAACA CCAGCGACCA GTCCTCCAGT CATATCCAAT 
AAAACATCTC CAGCTACTCC ACCAAGCACA ACTTCATCAT TTTCTATCAC AACAATTCAT 
CACAGTCCTG TGAAAAACCA TAGTCCTCTG GCAGGACAGA TGCCTACAGC AGTCACTCCT 
GGACTCAGTC CTTCATCAAA CCAGGTTCCT GTATCCTTTC TATCTGATGA AATGCTACCT 
TCTGAAACAA ATAAAATGAA TTCTGTCATA CTGAGTGAAG CATTTACAAC AGGAATTCCT 
GTTACTCCAC CATCTGAAAA ACCCAAGGAA ATTTCTAGAC CACCACATCT TCACCAGCGT 
TCTGAATCCT TTCCTTCACA TCCTGCATAT AGCAATGCAA AGCCCACTCC ACCAGTTAGA 
ACAATGGATA GTCGACTGGC AACTGCCATG CATTCCAACT TCAATGATGC CATCACTGCT 
AATAACTACC ACTCCTTCCT GAACACCATG ATGCTTCCTT CTTCTCTAGA GGATGTGCTA 
CCCAGACACA ACTATTCTCA TTCTTCTCAT CTCAAGACAG AAAATATAGA TGAACAGGGA 
GTTAATTACA GACAACCTTA TTTATCCAAC ACCAAGCAAG AGGACCCAGC TGATGTGGGA 
GATTTATACA GACAGCAATA TATTTCTACT GGAAAGTCTG AGAATGCTGA GATAGGAAAC 
ACTTACCGGC ACCCATATCC TTCCAATACT GAGCCAGAAA GTCTAAAGGA GCCACTAGAT 
CCTTTTCTGC ATCACAAGTC AGCAGTTGCT CCAAAGACAT ATAGAGCAGA AACACTTCTA 
CCACATATGC CTCCTCAAGT TTATGGTTCA AGATGTGATA CTCCATCCTC AACATTTTAT 
GGCACCTACA TAAATCAGGC CAAACACCCC CGCACCAGGA ACAGACCTGA TTATATGTCC 
TCTATGAGCC CAGGTCTCCG TAGCTACTCA GAAGACACAT CTTCCTACCC TACTATTCGA 
AGGGTACAAT CTCTGCATGT GCCTATGCAA CCTCCGCCAC CTCCTATCCG CACTGTTCCA 
ATTTCAAGAA CAGAGGTTCC CCCAGATGAT GATCCAGCAT ACTGCCCAAG ACCAGTTTAT 
CAATACAAAC AATGCCCCCA ATTTAACTCT CAGTCAGATT ACCATGTTAC CCAGCTGCAG 
CCTTACTTTG AAAATGGACG AGTTCATTAT CGCTACAGTC CCTATTCAGG GGGCCCAAGT 
TATTTTTCAG CAGATAGCAA TTTCTATGAT ATGGACCCCT ACAGCACCTT ACGTCTGCGA 
CAACTTCCTC TCTACCCAAG CCGGGACTTT GCTTCTTACA CCACAAGATT GCAGCCCAAA 
AACACATATC GGTCACAAGG ATTGCCCCCA TATCCCAGAG GAACTCTACG GGAACACAAT 
TTTATCAGTA GAGATGTTCC TCCTGCACTA CCACCTGAAC ATCCACGACC TCTTCACATT 
TCTTGGGACA TGGAAGACAT GGACAGATAC AGACTGCAAT CCCTGCGACG AGAGAACAGG 
GCACGTCAGA GAGCTAAAGG ACCAGTAATG TCTCAGTATG ATAATGTTAC ACCAACCTTA 
GTGGAGGATG TAGCAGGCTT AGATGTCATA CATCTCCGAA GCCGTTCTGA TCCTGGTAAG 
ACTCCTGGTC TTTTAAGTGT GGCTGAGAAC AAAGACGTCC GTTACCCAGG AAGGGCAGAA 
GGTGATGAGC ATACAACATA TCCACCTCCT GTTTTTGGAA ATGGCCATCA AGATAAGCCA 
TCTCTTCCTC AAAAGCAAAG TGGATCAAGC CGGGTTCGAA TGCAACATGA AATATCTACT 
GAGCAGCGGA CTCAACCTTC TCAAGATACT TTGCATAGGC AACCCACAGA GGGACGAAAT 
GGTCCTCCAA TTCCTCCTGT GGACTACAGC TCTAAAGGAA TGCCAAATCC CCCAGACCCT 
GCATCATATC ATTCCTCTGC AAACAAATAT AACTTGACAC AACAGGAACC CATGCGACTT 
ATTCACAAAG AGCTTCGGCT CCCTGAGGAT ATTGAGCATT CACATACCAG GCCAGCTGAC 
AACCAACACC GTGACTGTTA CAGGGAGGAG CCAGCTCAGT TTCCCCTTAC TGCACCTCCT 
CCTAAACCAG AGAGGAGCCA TAGCCTTCGA GGTCATAATC CAGATGTTTT AGAAAGAGAC 
CCTAGTATCT TCTACCCCTA CCAAACGCTT CAAGCCAAGA GACACAGCAC AATTAATGCA 
GTGTCACAAT ATGATAATCT AGCTGATTAC CATTCTATAC CCCATCATCG AACCACTAAC 
CAGTCTACTC CAAATGCCTT TAACCTCCCA CATGGACGGA CCTATGCTAC TGCCCTGGGC 
CAGGGTGCCT TTCTAGCTGC TGAACTCGGC TTGCAAAGGC CTGAGACAAA AATCCATGTG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951418.1
CDS	201..6086
Translation	MYRIMKSSLHLEEDDFVPELPRSIHPRERPDWEETISAMARGADIPVPGEIPTMPTEIPLRSCGSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGSIQLTLTEDQNEVSRNGCESKELVFLVQIACQGRSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLSELPRSDSVKDSPELVTQMLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINVPAIAAAHVIKRYNAQAPDELSFEVGDIVSVIDMPPKELTTWWRGKHGFQVGFFPSECVELINDKVPQSMTNSVPKPVSPAHGMRPASWSYFPPYLEMDSLRPGTSCCSEVLSHSQLSSVSRKHGKLITFLRTFMKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSGAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_018095929.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095929.1

ORF Insert Sequence:

ATGTACAGAA TAATGAAGTC TTCACTGCAC TTAGAGGAGG ATGATTTTGT ACCCGAGTTG 
CCAAGGAGTA TCCATCCCCG GGAGAGGCCG GACTGGGAGG AGACTATTAG TGCCATGGCA 
CGAGGGGCAG ACATCCCAGT CCCAGGGGAA ATACCGACGA TGCCTACAGA AATCCCTCTT 
CGCTCCTGTG GCAGTACTGC CAGCATGAAG GTGAAAAATG TCAAGAAGTT ATCCTTCACG 
AAAGGGCATT TTCCTAAACT TGCAGAATGT GCACATTTCC ACTATGAAAA TGTAGACTTT 
GGCAGCATAC AGCTCACACT CACAGAAGAT CAAAATGAAG TGAGCAGAAA TGGCTGTGAA 
TCCAAGGAAC TTGTTTTCCT AGTACAGATT GCTTGTCAGG GCCGTAGCTG GATAGTTAAG 
CGGAGTTATG AAGATTTTCG GGTTCTGGAT AAACACCTTC ATCTGTGCAT ATATGATAGG 
CGCTTCTCTC AGCTCTCAGA GCTGCCCCGT TCCGACTCCG TAAAGGATAG TCCAGAGTTG 
GTCACACAGA TGCTGATGGC TTACCTATCG CGGCTGTCAG CCATCGCAGG TAACAAGATC 
AATTGTGGGC CAGCTCTGAC GTGGATGGAG ATCGATAATA AAGGCAATCA TTTGCTTGTG 
CACGAAGAGT CCTCCATAAA TGTTCCAGCC ATCGCTGCTG CCCATGTCAT AAAGCGATAT 
AATGCTCAGG CCCCAGATGA ACTCTCTTTT GAGGTGGGAG ACATAGTATC TGTAATTGAC 
ATGCCACCAA AAGAACTGAC CACGTGGTGG AGAGGAAAGC ATGGATTTCA AGTGGGTTTC 
TTTCCAAGTG AATGTGTGGA ATTAATAAAT GATAAAGTCC CCCAATCAAT GACAAACTCT 
GTGCCTAAAC CAGTCTCCCC TGCGCATGGA ATGAGGCCGG CCTCCTGGAG CTACTTTCCC 
CCCTATTTGG AGATGGATAG TTTAAGGCCT GGAACATCGT GCTGTTCTGA GGTATTAAGT 
CACTCGCAGC TAAGCTCAGT GTCCCGGAAG CACGGCAAGC TCATCACATT TCTAAGAACC 
TTCATGAAGT CCAGACCCAG CAAACAGAAA CTGAAGCAGA GAGGGATCCT GAGGGAGCGA 
GTGTTTGGTT GTGACCTTGG GGAACATCTG CTTAACTCTG GTCAGGATGT CCCCCAAGTC 
CTCAGGAGCT GCACAGAGTT TATAGAAAAA CATGGCATTG TTGATGGAAT CTACCGTCTG 
TCTGGGATTG CATCTAACAT CCAGAAACTA CGTCATGAAT TTGACTCTGA ACAGATTCCA 
GACCTGACTA AAGATGTGTA CATCCAGGAC ATTCACTGTG TTGGCTCTCT CTGTAAACTC 
TACTTCCGGG AACTCCCAAA CCCTCTGCTC ACATATCAGT TGTATGAAAA GTTCTCTGAT 
GCTGTGTCAG CGGCTACTGA TGAAGAGAGA CTGGTGAAAA TTCATGATGT TATTCAACAG 
CTTCCCCCAC CTCATTACAG AACACTGGAG TTCCTTATGC GCCACTTATC AAGACTTGCG 
ACATACTGCT CTATCACCAA TATGCACACT AAAAATTTGG CAATTGTCTG GGCCCCTAAC 
CTGCTCCGGT CAAAACAGAT TGAGTCTGCA TGTTTTAGCG GTACAGCAGC TTTCATGGAA 
GTGCGCATAC AGTCTGTGGT AGTTGAATTT ATTCTAAACC ATGTGGAGGT TCTCTTTAGT 
CCTAAACTTA GTTCTGTGAT AAGGGATGGT GCTGGTCACA CTTCTTTGTC TCGGCCCAAG 
TCACTGTTGG TGTCATCTCC ATCTACAAAA CTACTAAGCC TAGAGGAGGC TCAAGCAAGG 
ACACAGGCCC AAATCAATTC CCCTGTTGTG GCTGACAGCA AGTACATTGA GGTGGGAGAG 
GGACCAGCTG CTTTGCAGGG CAGATTCCAC ACTGTAATTG ACTTCCCTTC TGAAAGGAAA 
AGACCGCCAA CCAAAATGAA AAAATCTCCT GTTGGAAGCT GGCGTTCTTT TTTTAGCATA 
GGCAAATCAT CATCTTCAGC CTCCATGTCT AAGCGGAAAC TTCAGCGTAA TCCAAGTGAG 
CCTTTGGAAA TGAAATCCAT GGCATTTGCA GGTGGAAGAG ATAGCAGCAC CATGCGCTCA 
GCTAAAAGCG AGGAGTCTCT CAGTTCCTTG CATGCAGCAG ATGGAGAGTC GAAGCTATTT 
CGTCCACGTC GTCCTCGTTC CAGCAGCGAT GCTCTGTCTG CTTCATATAA TGGGGACCTT 
CTGGATAGCT GCAACCGTTG CAACTCCTAT GATAATCTGC CCCGAGACCA AGACAGTGAT 
GGAGATGAGG GTTTGATTCA TGTGCCTGCT ATGCTGTCAG GTCGCTCTCC TGAGGATGAA 
GAACTCAGCC CACCAGACAT TGGTATGGCC AGTTTGGACT TTGACCCAAT GTCTTTTCAG 
TGCAGCCCTC CCCAGCTAGA ATCTGAAGGA CCAGACATTT TTTTTCAGCT TGACATTTGT 
GCTAGCAATG GCAAGGATAA GGTGCAAAGT GGTGCACAAA ACCTAGGCGG TGTCTCCAGC 
TCCGAACCTC ACTCTCCTTT CCAAGAGAAA GTCGTTAGTC CATTTCTATC GCCTGACCGC 
AGTTCTAGTA CAGATAAAGT ATCCAAAACT ACATCATTTG CAGAAAAAGT TGTGCAGGCC 
TTGTCACCTA AAGTGGGGCG CAAAGCAGTT CGATCTCCTC CATTAACCAT CTCAGAGCCT 
GTCACCATCT CTCTCCCTAG TCGGGTATCG GAAATGATTG GAAGTATGCA AGGCAATACT 
GCTGCTTCCC AGTCTTTAAT TTGGTACAGA GAAAACAGTT TAAATTCTAG AGAAACTGAA 
ATGAATTATG TTTGTAAATC GGTGGATGCC AACCTTCCAT CCTTATTGCC AGCAACAGAT 
CCATGGTCTA CAGTTACTCC TGTAATTACT CAATGGAACC CAGATGGATT ACAGAGAGAA 
GACAAAGCAG GACCATTTAC TCATGGCTTT CCCCAGCCAA ATAGTGACTT GGAACAAGTA 
GAAAACTTCA ACCTGGAACT TAGCACAACA AATGTTGCTG CAGCATATCA GAAAGAAGAT 
ACAGAATTGT CGAGAGCAAA TCAGAACAAA TCCCACTCTT CTGTTTCTCC CCCTCAAGGA 
GCTTCTGCTT CAGAATCTCC ACAGGAGCTA GCCCCTGTCA GTAGTGTTTC TATCATTCCA 
CCTCCACCTC CTCCAAAGAA TCCTGCACGC ATGCTGGCAT TAGCACTGGC TGAATCTGCT 
CAACAGGCAT CTGCTAATAA AAGACCTCTT TATATGCAGT TCATGGATCT ACCCCCCCAT 
CCTCCTCCAC CATCAGTACT AGAGGACAAA CCACTTCTTG AGTTATCTCC AAAAATGTCT 
CCAGAGCACC TCGTATCTGG TCCTGCAACA CCAGCGACCA GTCCTCCAGT CATATCCAAT 
AAAACATCTC CAGCTACTCC ACCAAGCACA ACTTCATCAT TTTCTATCAC AACAATTCAT 
CACAGTCCTG TGAAAAACCA TAGTCCTCTG GCAGGACAGA TGCCTACAGC AGTCACTCCT 
GGACTCAGTC CTTCATCAAA CCAGGTTCCT GTATCCTTTC TATCTGATGA AATGCTACCT 
TCTGAAACAA ATAAAATGAA TTCTGTCATA CTGAGTGAAG CATTTACAAC AGGAATTCCT 
GTTACTCCAC CATCTGAAAA ACCCAAGGAA ATTTCTAGAC CACCACATCT TCACCAGCGT 
TCTGAATCCT TTCCTTCACA TCCTGCATAT AGCAATGCAA AGCCCACTCC ACCAGTTAGA 
ACAATGGATA GTCGACTGGC AACTGCCATG CATTCCAACT TCAATGATGC CATCACTGCT 
AATAACTACC ACTCCTTCCT GAACACCATG ATGCTTCCTT CTTCTCTAGA GGATGTGCTA 
CCCAGACACA ACTATTCTCA TTCTTCTCAT CTCAAGACAG AAAATATAGA TGAACAGGGA 
GTTAATTACA GACAACCTTA TTTATCCAAC ACCAAGCAAG AGGACCCAGC TGATGTGGGA 
GATTTATACA GACAGCAATA TATTTCTACT GGAAAGTCTG AGAATGCTGA GATAGGAAAC 
ACTTACCGGC ACCCATATCC TTCCAATACT GAGCCAGAAA GTCTAAAGGA GCCACTAGAT 
CCTTTTCTGC ATCACAAGTC AGCAGTTGCT CCAAAGACAT ATAGAGCAGA AACACTTCTA 
CCACATATGC CTCCTCAAGT TTATGGTTCA AGATGTGATA CTCCATCCTC AACATTTTAT 
GGCACCTACA TAAATCAGGC CAAACACCCC CGCACCAGGA ACAGACCTGA TTATATGTCC 
TCTATGAGCC CAGGTCTCCG TAGCTACTCA GAAGACACAT CTTCCTACCC TACTATTCGA 
AGGGTACAAT CTCTGCATGT GCCTATGCAA CCTCCGCCAC CTCCTATCCG CACTGTTCCA 
ATTTCAAGAA CAGAGGTTCC CCCAGATGAT GATCCAGCAT ACTGCCCAAG ACCAGTTTAT 
CAATACAAAC AATGCCCCCA ATTTAACTCT CAGTCAGATT ACCATGTTAC CCAGCTGCAG 
CCTTACTTTG AAAATGGACG AGTTCATTAT CGCTACAGTC CCTATTCAGG GGGCCCAAGT 
TATTTTTCAG CAGATAGCAA TTTCTATGAT ATGGACCCCT ACAGCACCTT ACGTCTGCGA 
CAACTTCCTC TCTACCCAAG CCGGGACTTT GCTTCTTACA CCACAAGATT GCAGCCCAAA 
AACACATATC GGTCACAAGG ATTGCCCCCA TATCCCAGAG GAACTCTACG GGAACACAAT 
TTTATCAGTA GAGATGTTCC TCCTGCACTA CCACCTGAAC ATCCACGACC TCTTCACATT 
TCTTGGGACA TGGAAGACAT GGACAGATAC AGACTGCAAT CCCTGCGACG AGAGAACAGG 
GCACGTCAGA GAGCTAAAGG ACCAGTAATG TCTCAGTATG ATAATGTTAC ACCAACCTTA 
GTGGAGGATG TAGCAGGCTT AGATGTCATA CATCTCCGAA GCCGTTCTGA TCCTGGTAAG 
ACTCCTGGTC TTTTAAGTGT GGCTGAGAAC AAAGACGTCC GTTACCCAGG AAGGGCAGAA 
GGTGATGAGC ATACAACATA TCCACCTCCT GTTTTTGGAA ATGGCCATCA AGATAAGCCA 
TCTCTTCCTC AAAAGCAAAG TGGATCAAGC CGGGTTCGAA TGCAACATGA AATATCTACT 
GAGCAGCGGA CTCAACCTTC TCAAGATACT TTGCATAGGC AACCCACAGA GGGACGAAAT 
GGTCCTCCAA TTCCTCCTGT GGACTACAGC TCTAAAGGAA TGCCAAATCC CCCAGACCCT 
GCATCATATC ATTCCTCTGC AAACAAATAT AACTTGACAC AACAGGAACC CATGCGACTT 
ATTCACAAAG AGCTTCGGCT CCCTGAGGAT ATTGAGCATT CACATACCAG GCCAGCTGAC 
AACCAACACC GTGACTGTTA CAGGGAGGAG CCAGCTCAGT TTCCCCTTAC TGCACCTCCT 
CCTAAACCAG AGAGGAGCCA TAGCCTTCGA GGTCATAATC CAGATGTTTT AGAAAGAGAC 
CCTAGTATCT TCTACCCCTA CCAAACGCTT CAAGCCAAGA GACACAGCAC AATTAATGCA 
GTGTCACAAT ATGATAATCT AGCTGATTAC CATTCTATAC CCCATCATCG AACCACTAAC 
CAGTCTACTC CAAATGCCTT TAACCTCCCA CATGGACGGA CCTATGCTAC TGCCCTGGGC 
CAGGGTGCCT TTCTAGCTGC TGAACTCGGC TTGCAAAGGC CTGAGACAAA AATCCATGTG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_018095931.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951420.1 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X9, mRNA.

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CloneID	OXa45815
Clone ID Related Accession (Same CDS sequence)	XM_018095931.1
Accession Version	XM_018095931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATGG CTTACCTATC GCGGCTGTCA GCCATCGCAG GTAACAAGAT CAATTGTGGG 
CCAGCTCTGA CGTGGATGGA GATCGATAAT AAAGGCAATC ATTTGCTTGT GCACGAAGAG 
TCCTCCATAA ATGTTCCAGC CATCGCTGCT GCCCATGTCA TAAAGCGATA TAATGCTCAG 
GCCCCAGATG AACTCTCTTT TGAGGTGGGA GACATAGTAT CTGTAATTGA CATGCCACCA 
AAAGAACTGA CCACGTGGTG GAGAGGAAAG CATGGATTTC AAGTGGGTTT CTTTCCAAGT 
GAATGTGTGG AATTAATAAA TGATAAAGTC CCCCAATCAA TGACAAACTC TGTGCCTAAA 
CCAGATTTGG AGATGGATAG TTTAAGGCCT GGAACATCGT GCTGTTCTGA GGTATTAAGT 
CACTCGCAGC TAAGCTCAGT GTCCCGGAAG CACGGCAAGC TCATCACATT TCTAAGAACC 
TTCATGAAGT CCAGACCCAG CAAACAGAAA CTGAAGCAGA GAGGGATCCT GAGGGAGCGA 
GTGTTTGGTT GTGACCTTGG GGAACATCTG CTTAACTCTG GTCAGGATGT CCCCCAAGTC 
CTCAGGAGCT GCACAGAGTT TATAGAAAAA CATGGCATTG TTGATGGAAT CTACCGTCTG 
TCTGGGATTG CATCTAACAT CCAGAAACTA CGTCATGAAT TTGACTCTGA ACAGATTCCA 
GACCTGACTA AAGATGTGTA CATCCAGGAC ATTCACTGTG TTGGCTCTCT CTGTAAACTC 
TACTTCCGGG AACTCCCAAA CCCTCTGCTC ACATATCAGT TGTATGAAAA GTTCTCTGAT 
GCTGTGTCAG CGGCTACTGA TGAAGAGAGA CTGGTGAAAA TTCATGATGT TATTCAACAG 
CTTCCCCCAC CTCATTACAG AACACTGGAG TTCCTTATGC GCCACTTATC AAGACTTGCG 
ACATACTGCT CTATCACCAA TATGCACACT AAAAATTTGG CAATTGTCTG GGCCCCTAAC 
CTGCTCCGGT CAAAACAGAT TGAGTCTGCA TGTTTTAGCG GTACAGCAGC TTTCATGGAA 
GTGCGCATAC AGTCTGTGGT AGTTGAATTT ATTCTAAACC ATGTGGAGGT TCTCTTTAGT 
CCTAAACTTA GTTCTGTGAT AAGGGATGGT GCTGGTCACA CTTCTTTGTC TCGGCCCAAG 
TCACTGTTGG TGTCATCTCC ATCTACAAAA CTACTAAGCC TAGAGGAGGC TCAAGCAAGG 
ACACAGGCCC AAATCAATTC CCCTGTTGTG GCTGACAGCA AGTACATTGA GGTGGGAGAG 
GGACCAGCTG CTTTGCAGGG CAGATTCCAC ACTGTAATTG ACTTCCCTTC TGAAAGGAAA 
AGACCGCCAA CCAAAATGAA AAAATCTCCT GTTGGAAGCT GGCGTTCTTT TTTTAGCATA 
GGCAAATCAT CATCTTCAGC CTCCATGTCT AAGCGGAAAC TTCAGCGTAA TCCAAGTGAG 
CCTTTGGAAA TGAAATCCAT GGCATTTGCA GGTGGAAGAG ATAGCAGCAC CATGCGCTCA 
GCTAAAAGCG AGGAGTCTCT CAGTTCCTTG CATGCAGCAG ATGGAGAGTC GAAGCTATTT 
CGTCCACGTC GTCCTCGTTC CAGCAGCGAT GCTCTGTCTG CTTCATATAA TGGGGACCTT 
CTGGATAGCT GCAACCGTTG CAACTCCTAT GATAATCTGC CCCGAGACCA AGACAGTGAT 
GGAGATGAGG GTTTGATTCA TGTGCCTGCT ATGCTGTCAG GTCGCTCTCC TGAGGATGAA 
GAACTCAGCC CACCAGACAT TGGTATGGCC AGTTTGGACT TTGACCCAAT GTCTTTTCAG 
TGCAGCCCTC CCCAGCTAGA ATCTGAAGGA CCAGACATTT TTTTTCAGCT TGACATTTGT 
GCTAGCAATG GCAAGGATAA GGTGCAAAGT GGTGCACAAA ACCTAGGCGG TGTCTCCAGC 
TCCGAACCTC ACTCTCCTTT CCAAGAGAAA GTCGTTAGTC CATTTCTATC GCCTGACCGC 
AGTTCTAGTA CAGATAAAGT ATCCAAAACT ACATCATTTG CAGAAAAAGT TGTGCAGGCC 
TTGTCACCTA AAGTGGGGCG CAAAGCAGTT CGATCTCCTC CATTAACCAT CTCAGAGCCT 
GTCACCATCT CTCTCCCTAG TCGGGTATCG GAAATGATTG GAAGTATGCA AGGCAATACT 
GCTGCTTCCC AGTCTTTAAT TTGGTACAGA GAAAACAGTT TAAATTCTAG AGAAACTGAA 
ATGAATTATG TTTGTAAATC GGTGGATGCC AACCTTCCAT CCTTATTGCC AGCAACAGAT 
CCATGGTCTA CAGTTACTCC TGTAATTACT CAATGGAACC CAGATGGATT ACAGAGAGAA 
GACAAAGCAG GACCATTTAC TCATGGCTTT CCCCAGCCAA ATAGTGACTT GGAACAAGTA 
GAAAACTTCA ACCTGGAACT TAGCACAACA AATGTTGCTG CAGCATATCA GAAAGAAGAT 
ACAGAATTGT CGAGAGCAAA TCAGAACAAA TCCCACTCTT CTGTTTCTCC CCCTCAAGGA 
GCTTCTGCTT CAGAATCTCC ACAGGAGCTA GCCCCTGTCA GTAGTGTTTC TATCATTCCA 
CCTCCACCTC CTCCAAAGAA TCCTGCACGC ATGCTGGCAT TAGCACTGGC TGAATCTGCT 
CAACAGGCAT CTGCTAATAA AAGACCTCTT TATATGCAGT TCATGGATCT ACCCCCCCAT 
CCTCCTCCAC CATCAGTACT AGAGGACAAA CCACTTCTTG AGTTATCTCC AAAAATGTCT 
CCAGAGCACC TCGTATCTGG TCCTGCAACA CCAGCGACCA GTCCTCCAGT CATATCCAAT 
AAAACATCTC CAGCTACTCC ACCAAGCACA ACTTCATCAT TTTCTATCAC AACAATTCAT 
CACAGTCCTG TGAAAAACCA TAGTCCTCTG GCAGGACAGA TGCCTACAGC AGTCACTCCT 
GGACTCAGTC CTTCATCAAA CCAGGTTCCT GTATCCTTTC TATCTGATGA AATGCTACCT 
TCTGAAACAA ATAAAATGAA TTCTGTCATA CTGAGTGAAG CATTTACAAC AGGAATTCCT 
GTTACTCCAC CATCTGAAAA ACCCAAGGAA ATTTCTAGAC CACCACATCT TCACCAGCGT 
TCTGAATCCT TTCCTTCACA TCCTGCATAT AGCAATGCAA AGCCCACTCC ACCAGTTAGA 
ACAATGGATA GTCGACTGGC AACTGCCATG CATTCCAACT TCAATGATGC CATCACTGCT 
AATAACTACC ACTCCTTCCT GAACACCATG ATGCTTCCTT CTTCTCTAGA GGATGTGCTA 
CCCAGACACA ACTATTCTCA TTCTTCTCAT CTCAAGACAG AAAATATAGA TGAACAGGGA 
GTTAATTACA GACAACCTTA TTTATCCAAC ACCAAGCAAG AGGACCCAGC TGATGTGGGA 
GATTTATACA GACAGCAATA TATTTCTACT GGAAAGTCTG AGAATGCTGA GATAGGAAAC 
ACTTACCGGC ACCCATATCC TTCCAATACT GAGCCAGAAA GTCTAAAGGA GCCACTAGAT 
CCTTTTCTGC ATCACAAGTC AGCAGTTGCT CCAAAGACAT ATAGAGCAGA AACACTTCTA 
CCACATATGC CTCCTCAAGT TTATGGTTCA AGATGTGATA CTCCATCCTC AACATTTTAT 
GGCACCTACA TAAATCAGGC CAAACACCCC CGCACCAGGA ACAGACCTGA TTATATGTCC 
TCTATGAGCC CAGGTCTCCG TAGCTACTCA GAAGACACAT CTTCCTACCC TACTATTCGA 
AGGGTACAAT CTCTGCATGT GCCTATGCAA CCTCCGCCAC CTCCTATCCG CACTGTTCCA 
ATTTCAAGAA CAGAGGTTCC CCCAGATGAT GATCCAGCAT ACTGCCCAAG ACCAGTTTAT 
CAATACAAAC AATGCCCCCA ATTTAACTCT CAGTCAGATT ACCATGTTAC CCAGCTGCAG 
CCTTACTTTG AAAATGGACG AGTTCATTAT CGCTACAGTC CCTATTCAGG GGGCCCAAGT 
TATTTTTCAG CAGATAGCAA TTTCTATGAT ATGGACCCCT ACAGCACCTT ACGTCTGCGA 
CAACTTCCTC TCTACCCAAG CCGGGACTTT GCTTCTTACA CCACAAGATT GCAGCCCAAA 
AACACATATC GGTCACAAGG ATTGCCCCCA TATCCCAGAG GAACTCTACG GGAACACAAT 
TTTATCAGTA GAGATGTTCC TCCTGCACTA CCACCTGAAC ATCCACGACC TCTTCACATT 
TCTTGGGACA TGGAAGACAT GGACAGATAC AGACTGCAAT CCCTGCGACG AGAGAACAGG 
GCACGTCAGA GAGCTAAAGG ACCAGTAATG TCTCAGTATG ATAATGTTAC ACCAACCTTA 
GTGGAGGATG TAGCAGGCTT AGATGTCATA CATCTCCGAA GCCGTTCTGA TCCTGGTAAG 
ACTCCTGGTC TTTTAAGTGT GGCTGAGAAC AAAGACGTCC GTTACCCAGG AAGGGCAGAA 
GGTGATGAGC ATACAACATA TCCACCTCCT GTTTTTGGAA ATGGCCATCA AGATAAGCCA 
TCTCTTCCTC AAAAGCAAAG TGGATCAAGC CGGGTTCGAA TGCAACATGA AATATCTACT 
GAGCAGCGGA CTCAACCTTC TCAAGATACT TTGCATAGGC AACCCACAGA GGGACGAAAT 
GGTCCTCCAA TTCCTCCTGT GGACTACAGC TCTAAAGGAA TGCCAAATCC CCCAGACCCT 
GCATCATATC ATTCCTCTGC AAACAAATAT AACTTGACAC AACAGGAACC CATGCGACTT 
ATTCACAAAG AGCTTCGGCT CCCTGAGGAT ATTGAGCATT CACATACCAG GCCAGCTGAC 
AACCAACACC GTGACTGTTA CAGGGAGGAG CCAGCTCAGT TTCCCCTTAC TGCACCTCCT 
CCTAAACCAG AGAGGAGCCA TAGCCTTCGA GGTCATAATC CAGATGTTTT AGAAAGAGAC 
CCTAGTATCT TCTACCCCTA CCAAACGCTT CAAGCCAAGA GACACAGCAC AATTAATGCA 
GTGTCACAAT ATGATAATCT AGCTGATTAC CATTCTATAC CCCATCATCG AACCACTAAC 
CAGTCTACTC CAAATGCCTT TAACCTCCCA CATGGACGGA CCTATGCTAC TGCCCTGGGC 
CAGGGTGCCT TTCTAGCTGC TGAACTCGGC TTGCAAAGGC CTGAGACAAA AATCCATGTG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951420.1
CDS	592..5877
Translation	MLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINVPAIAAAHVIKRYNAQAPDELSFEVGDIVSVIDMPPKELTTWWRGKHGFQVGFFPSECVELINDKVPQSMTNSVPKPDLEMDSLRPGTSCCSEVLSHSQLSSVSRKHGKLITFLRTFMKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSGAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_018095931.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095931.1

ORF Insert Sequence:

ATGCTGATGG CTTACCTATC GCGGCTGTCA GCCATCGCAG GTAACAAGAT CAATTGTGGG 
CCAGCTCTGA CGTGGATGGA GATCGATAAT AAAGGCAATC ATTTGCTTGT GCACGAAGAG 
TCCTCCATAA ATGTTCCAGC CATCGCTGCT GCCCATGTCA TAAAGCGATA TAATGCTCAG 
GCCCCAGATG AACTCTCTTT TGAGGTGGGA GACATAGTAT CTGTAATTGA CATGCCACCA 
AAAGAACTGA CCACGTGGTG GAGAGGAAAG CATGGATTTC AAGTGGGTTT CTTTCCAAGT 
GAATGTGTGG AATTAATAAA TGATAAAGTC CCCCAATCAA TGACAAACTC TGTGCCTAAA 
CCAGATTTGG AGATGGATAG TTTAAGGCCT GGAACATCGT GCTGTTCTGA GGTATTAAGT 
CACTCGCAGC TAAGCTCAGT GTCCCGGAAG CACGGCAAGC TCATCACATT TCTAAGAACC 
TTCATGAAGT CCAGACCCAG CAAACAGAAA CTGAAGCAGA GAGGGATCCT GAGGGAGCGA 
GTGTTTGGTT GTGACCTTGG GGAACATCTG CTTAACTCTG GTCAGGATGT CCCCCAAGTC 
CTCAGGAGCT GCACAGAGTT TATAGAAAAA CATGGCATTG TTGATGGAAT CTACCGTCTG 
TCTGGGATTG CATCTAACAT CCAGAAACTA CGTCATGAAT TTGACTCTGA ACAGATTCCA 
GACCTGACTA AAGATGTGTA CATCCAGGAC ATTCACTGTG TTGGCTCTCT CTGTAAACTC 
TACTTCCGGG AACTCCCAAA CCCTCTGCTC ACATATCAGT TGTATGAAAA GTTCTCTGAT 
GCTGTGTCAG CGGCTACTGA TGAAGAGAGA CTGGTGAAAA TTCATGATGT TATTCAACAG 
CTTCCCCCAC CTCATTACAG AACACTGGAG TTCCTTATGC GCCACTTATC AAGACTTGCG 
ACATACTGCT CTATCACCAA TATGCACACT AAAAATTTGG CAATTGTCTG GGCCCCTAAC 
CTGCTCCGGT CAAAACAGAT TGAGTCTGCA TGTTTTAGCG GTACAGCAGC TTTCATGGAA 
GTGCGCATAC AGTCTGTGGT AGTTGAATTT ATTCTAAACC ATGTGGAGGT TCTCTTTAGT 
CCTAAACTTA GTTCTGTGAT AAGGGATGGT GCTGGTCACA CTTCTTTGTC TCGGCCCAAG 
TCACTGTTGG TGTCATCTCC ATCTACAAAA CTACTAAGCC TAGAGGAGGC TCAAGCAAGG 
ACACAGGCCC AAATCAATTC CCCTGTTGTG GCTGACAGCA AGTACATTGA GGTGGGAGAG 
GGACCAGCTG CTTTGCAGGG CAGATTCCAC ACTGTAATTG ACTTCCCTTC TGAAAGGAAA 
AGACCGCCAA CCAAAATGAA AAAATCTCCT GTTGGAAGCT GGCGTTCTTT TTTTAGCATA 
GGCAAATCAT CATCTTCAGC CTCCATGTCT AAGCGGAAAC TTCAGCGTAA TCCAAGTGAG 
CCTTTGGAAA TGAAATCCAT GGCATTTGCA GGTGGAAGAG ATAGCAGCAC CATGCGCTCA 
GCTAAAAGCG AGGAGTCTCT CAGTTCCTTG CATGCAGCAG ATGGAGAGTC GAAGCTATTT 
CGTCCACGTC GTCCTCGTTC CAGCAGCGAT GCTCTGTCTG CTTCATATAA TGGGGACCTT 
CTGGATAGCT GCAACCGTTG CAACTCCTAT GATAATCTGC CCCGAGACCA AGACAGTGAT 
GGAGATGAGG GTTTGATTCA TGTGCCTGCT ATGCTGTCAG GTCGCTCTCC TGAGGATGAA 
GAACTCAGCC CACCAGACAT TGGTATGGCC AGTTTGGACT TTGACCCAAT GTCTTTTCAG 
TGCAGCCCTC CCCAGCTAGA ATCTGAAGGA CCAGACATTT TTTTTCAGCT TGACATTTGT 
GCTAGCAATG GCAAGGATAA GGTGCAAAGT GGTGCACAAA ACCTAGGCGG TGTCTCCAGC 
TCCGAACCTC ACTCTCCTTT CCAAGAGAAA GTCGTTAGTC CATTTCTATC GCCTGACCGC 
AGTTCTAGTA CAGATAAAGT ATCCAAAACT ACATCATTTG CAGAAAAAGT TGTGCAGGCC 
TTGTCACCTA AAGTGGGGCG CAAAGCAGTT CGATCTCCTC CATTAACCAT CTCAGAGCCT 
GTCACCATCT CTCTCCCTAG TCGGGTATCG GAAATGATTG GAAGTATGCA AGGCAATACT 
GCTGCTTCCC AGTCTTTAAT TTGGTACAGA GAAAACAGTT TAAATTCTAG AGAAACTGAA 
ATGAATTATG TTTGTAAATC GGTGGATGCC AACCTTCCAT CCTTATTGCC AGCAACAGAT 
CCATGGTCTA CAGTTACTCC TGTAATTACT CAATGGAACC CAGATGGATT ACAGAGAGAA 
GACAAAGCAG GACCATTTAC TCATGGCTTT CCCCAGCCAA ATAGTGACTT GGAACAAGTA 
GAAAACTTCA ACCTGGAACT TAGCACAACA AATGTTGCTG CAGCATATCA GAAAGAAGAT 
ACAGAATTGT CGAGAGCAAA TCAGAACAAA TCCCACTCTT CTGTTTCTCC CCCTCAAGGA 
GCTTCTGCTT CAGAATCTCC ACAGGAGCTA GCCCCTGTCA GTAGTGTTTC TATCATTCCA 
CCTCCACCTC CTCCAAAGAA TCCTGCACGC ATGCTGGCAT TAGCACTGGC TGAATCTGCT 
CAACAGGCAT CTGCTAATAA AAGACCTCTT TATATGCAGT TCATGGATCT ACCCCCCCAT 
CCTCCTCCAC CATCAGTACT AGAGGACAAA CCACTTCTTG AGTTATCTCC AAAAATGTCT 
CCAGAGCACC TCGTATCTGG TCCTGCAACA CCAGCGACCA GTCCTCCAGT CATATCCAAT 
AAAACATCTC CAGCTACTCC ACCAAGCACA ACTTCATCAT TTTCTATCAC AACAATTCAT 
CACAGTCCTG TGAAAAACCA TAGTCCTCTG GCAGGACAGA TGCCTACAGC AGTCACTCCT 
GGACTCAGTC CTTCATCAAA CCAGGTTCCT GTATCCTTTC TATCTGATGA AATGCTACCT 
TCTGAAACAA ATAAAATGAA TTCTGTCATA CTGAGTGAAG CATTTACAAC AGGAATTCCT 
GTTACTCCAC CATCTGAAAA ACCCAAGGAA ATTTCTAGAC CACCACATCT TCACCAGCGT 
TCTGAATCCT TTCCTTCACA TCCTGCATAT AGCAATGCAA AGCCCACTCC ACCAGTTAGA 
ACAATGGATA GTCGACTGGC AACTGCCATG CATTCCAACT TCAATGATGC CATCACTGCT 
AATAACTACC ACTCCTTCCT GAACACCATG ATGCTTCCTT CTTCTCTAGA GGATGTGCTA 
CCCAGACACA ACTATTCTCA TTCTTCTCAT CTCAAGACAG AAAATATAGA TGAACAGGGA 
GTTAATTACA GACAACCTTA TTTATCCAAC ACCAAGCAAG AGGACCCAGC TGATGTGGGA 
GATTTATACA GACAGCAATA TATTTCTACT GGAAAGTCTG AGAATGCTGA GATAGGAAAC 
ACTTACCGGC ACCCATATCC TTCCAATACT GAGCCAGAAA GTCTAAAGGA GCCACTAGAT 
CCTTTTCTGC ATCACAAGTC AGCAGTTGCT CCAAAGACAT ATAGAGCAGA AACACTTCTA 
CCACATATGC CTCCTCAAGT TTATGGTTCA AGATGTGATA CTCCATCCTC AACATTTTAT 
GGCACCTACA TAAATCAGGC CAAACACCCC CGCACCAGGA ACAGACCTGA TTATATGTCC 
TCTATGAGCC CAGGTCTCCG TAGCTACTCA GAAGACACAT CTTCCTACCC TACTATTCGA 
AGGGTACAAT CTCTGCATGT GCCTATGCAA CCTCCGCCAC CTCCTATCCG CACTGTTCCA 
ATTTCAAGAA CAGAGGTTCC CCCAGATGAT GATCCAGCAT ACTGCCCAAG ACCAGTTTAT 
CAATACAAAC AATGCCCCCA ATTTAACTCT CAGTCAGATT ACCATGTTAC CCAGCTGCAG 
CCTTACTTTG AAAATGGACG AGTTCATTAT CGCTACAGTC CCTATTCAGG GGGCCCAAGT 
TATTTTTCAG CAGATAGCAA TTTCTATGAT ATGGACCCCT ACAGCACCTT ACGTCTGCGA 
CAACTTCCTC TCTACCCAAG CCGGGACTTT GCTTCTTACA CCACAAGATT GCAGCCCAAA 
AACACATATC GGTCACAAGG ATTGCCCCCA TATCCCAGAG GAACTCTACG GGAACACAAT 
TTTATCAGTA GAGATGTTCC TCCTGCACTA CCACCTGAAC ATCCACGACC TCTTCACATT 
TCTTGGGACA TGGAAGACAT GGACAGATAC AGACTGCAAT CCCTGCGACG AGAGAACAGG 
GCACGTCAGA GAGCTAAAGG ACCAGTAATG TCTCAGTATG ATAATGTTAC ACCAACCTTA 
GTGGAGGATG TAGCAGGCTT AGATGTCATA CATCTCCGAA GCCGTTCTGA TCCTGGTAAG 
ACTCCTGGTC TTTTAAGTGT GGCTGAGAAC AAAGACGTCC GTTACCCAGG AAGGGCAGAA 
GGTGATGAGC ATACAACATA TCCACCTCCT GTTTTTGGAA ATGGCCATCA AGATAAGCCA 
TCTCTTCCTC AAAAGCAAAG TGGATCAAGC CGGGTTCGAA TGCAACATGA AATATCTACT 
GAGCAGCGGA CTCAACCTTC TCAAGATACT TTGCATAGGC AACCCACAGA GGGACGAAAT 
GGTCCTCCAA TTCCTCCTGT GGACTACAGC TCTAAAGGAA TGCCAAATCC CCCAGACCCT 
GCATCATATC ATTCCTCTGC AAACAAATAT AACTTGACAC AACAGGAACC CATGCGACTT 
ATTCACAAAG AGCTTCGGCT CCCTGAGGAT ATTGAGCATT CACATACCAG GCCAGCTGAC 
AACCAACACC GTGACTGTTA CAGGGAGGAG CCAGCTCAGT TTCCCCTTAC TGCACCTCCT 
CCTAAACCAG AGAGGAGCCA TAGCCTTCGA GGTCATAATC CAGATGTTTT AGAAAGAGAC 
CCTAGTATCT TCTACCCCTA CCAAACGCTT CAAGCCAAGA GACACAGCAC AATTAATGCA 
GTGTCACAAT ATGATAATCT AGCTGATTAC CATTCTATAC CCCATCATCG AACCACTAAC 
CAGTCTACTC CAAATGCCTT TAACCTCCCA CATGGACGGA CCTATGCTAC TGCCCTGGGC 
CAGGGTGCCT TTCTAGCTGC TGAACTCGGC TTGCAAAGGC CTGAGACAAA AATCCATGTG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_004916165.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916222.2 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X3, mRNA.

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CloneID	OXa61961
Clone ID Related Accession (Same CDS sequence)	XM_004916165.4 , XM_004916164.4 , XM_031905576.1
Accession Version	XM_004916165.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916165.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGTCTCC 
CCTGCGCATG GAATGAGGCC GGCCTCCTGG AGCTACTTTC CCCCCTATTT GGAGATGGAT 
AGTTTAAGGC CTGGAACATC GTGCTGTTCT GAGGTATTAA GTCACTCGCA GCTAAGCTCA 
GTGTCCCGGA AGCACGGCAA GCTCATCACA TTTCTAAGAA CCTTCATGAA GTCCAGACCC 
AGCAAACAGA AACTGAAGCA GAGAGGGATC CTGAGGGAGC GAGTGTTTGG TTGTGACCTT 
GGGGAACATC TGCTTAACTC TGGTCAGGAT GTCCCCCAAG TCCTCAGGAG CTGCACAGAG 
TTTATAGAAA AACATGGCAT TGTTGATGGA ATCTACCGTC TGTCTGGGAT TGCATCTAAC 
ATCCAGAAAC TACGTCATGA ATTTGACTCT GAACAGATTC CAGACCTGAC TAAAGATGTG 
TACATCCAGG ACATTCACTG TGTTGGCTCT CTCTGTAAAC TCTACTTCCG GGAACTCCCA 
AACCCTCTGC TCACATATCA GTTGTATGAA AAGTTCTCTG ATGCTGTGTC AGCGGCTACT 
GATGAAGAGA GACTGGTGAA AATTCATGAT GTTATTCAAC AGCTTCCCCC ACCTCATTAC 
AGAACACTGG AGTTCCTTAT GCGCCACTTA TCAAGACTTG CGACATACTG CTCTATCACC 
AATATGCACA CTAAAAATTT GGCAATTGTC TGGGCCCCTA ACCTGCTCCG GTCAAAACAG 
ATTGAGTCTG CATGTTTTAG CGGTACAGCA GCTTTCATGG AAGTGCGCAT ACAGTCTGTG 
GTAGTTGAAT TTATTCTAAA CCATGTGGAG GTTCTCTTTA GTCCTAAACT TAGTTCTGTG 
ATAAGGGATG GTGCTGGTCA CACTTCTTTG TCTCGGCCCA AGTCACTGTT GGTGTCATCT 
CCATCTACAA AACTACTAAG CCTAGAGGAG GCTCAAGCAA GGACACAGGC CCAAATCAAT 
TCCCCTGTTG TGGCTGACAG CAAGTACATT GAGGTGGGAG AGGGACCAGC TGCTTTGCAG 
GGCAGATTCC ACACTGTAAT TGACTTCCCT TCTGAAAGGA AAAGACCGCC AACCAAAATG 
AAAAAATCTC CTGTTGGAAG CTGGCGTTCT TTTTTTAGCA TAGGCAAATC ATCATCTTCA 
GCCTCCATGT CTAAGCGGAA ACTTCAGCGT AATCCAAGTG AGCCTTTGGA AATGAAATCC 
ATGGCATTTG CAGGTGGAAG AGATAGCAGC ACCATGCGCT CAGCTAAAAG CGAGGAGTCT 
CTCAGTTCCT TGCATGCAGC AGATGGAGAG TCGAAGCTAT TTCGTCCACG TCGTCCTCGT 
TCCAGCAGCG ATGCTCTGTC TGCTTCATAT AATGGGGACC TTCTGGATAG CTGCAACCGT 
TGCAACTCCT ATGATAATCT GCCCCGAGAC CAAGACAGTG ATGGAGATGA GGGTTTGATT 
CATGTGCCTG CTATGCTGTC AGGTCGCTCT CCTGAGGATG AAGAACTCAG CCCACCAGAC 
ATTGGTATGG CCAGTTTGGA CTTTGACCCA ATGTCTTTTC AGTGCAGCCC TCCCCAGCTA 
GAATCTGAAG GACCAGACAT TTTTTTTCAG CTTGACATTT GTGCTAGCAA TGGCAAGGAT 
AAGGTGCAAA GTGATGCACA AAACCTAGGC GGTGTCTCCA GCTCCGAACC TCACTCTCCT 
TTCCAAGAGA AAGTCGTTAG TCCATTTCTA TCGCCTGACC GCAGTTCTAG TACAGATAAA 
GTATCCAAAA CTACATCATT TGCAGAAAAA GTTGTGCAGG CCTTGTCACC TAAAGTGGGG 
CGCAAAGCAG TTCGATCTCC TCCATTAACC ATCTCAGAGC CTGTCACCAT CTCTCTCCCT 
AGTCGGGTAT CGGAAATGAT TGGAAGTATG CAAGGCAATA CTGCTGCTTC CCAGTCTTTA 
ATTTGGTACA GAGAAAACAG TTTAAATTCT AGAGAAACTG AAATGAATTA TGTTTGTAAA 
TCGGTGGATG CCAACCTTCC ATCCTTATTG CCAGCAACAG ATCCATGGTC TACAGTTACT 
CCTGTAATTA CTCAATGGAA CCCAGATGGA TTACAGAGAG AAGACAAAGC AGGACCATTT 
ACTCATGGCT TTCCCCAGCC AAATAGTGAC TTGGAACAAG TAGAAAACTT CAACCTGGAA 
CTTAGCACAA CAAATGTTGC TGCAGCATAT CAGAAAGAAG ATACAGAATT GTCGAGAGCA 
AATCAGAACA AATCCCACTC TTCTGTTTCT CCCCCTCAAG GAGCTTCTGC TTCAGAATCT 
CCACAGGAGC TAGCCCCTGT CAGTAGTGTT TCTATCATTC CACCTCCACC TCCTCCAAAG 
AATCCTGCAC GCATGCTGGC ATTAGCACTG GCTGAATCTG CTCAACAGGC ATCTGCTAAT 
AAAAGACCTC TTTATATGCA GTTCATGGAT CTACCCCCCC ATCCTCCTCC ACCATCAGTA 
CTAGAGGACA AACCACTTCT TGAGTTATCT CCAAAAATGT CTCCAGAGCA CCTTGTATCT 
GGTCCTGCAA CACCAGCGAC CAGTCCTCCA GTCATATCCA ATAAAACATC TCCAGCTACT 
CCACCAAGCA CAACTTCATC ATTTTCTATC ACAACAATTC ATCACAGTCC TGTGAAAAAC 
CATAGTCCTC TGGCAGGACA GATGCCTACA GCAGTCACTC CTGGACTCAG TCCTTCATCA 
AACCAGGTTC CTGTATCCTT TCTATCTGAT GAAATGCTAC CTTCTGAAAC AAATAAAATG 
AATTCTGTCA TACTGAGTGA AGCATTTACA ACAGGAATTC CTGTTACTCC ACCATCTGAA 
AAACCCAAGG AAATTTCTAG ACCACCACAT CTTCACCAGC GTTCTGAATC CTTTCCTTCA 
CATCCTGCAT ATAGCAATGC AAAGCCCACT CCACCAGTTA GAACAATGGA TAGTCGACTG 
GCAACTGCCA TGCATTCCAA CTTCAATGAT GCCATCACTG CTAATAACTA CCACTCCTTC 
CTGAACACCA TGATGCTTCC TTCTTCTCTA GAGGATGTGC TACCCAGACA CAACTATTCT 
CATTCTTCTC ATCTCAAGAC AGAAAATATA GATGAACAGG GAGTTAATTA CAGACAACCT 
TATTTATCCA ACACCAAGCA AGAGGACCCA GCTGATGTGG GAGATTTATA CAGACAGCAA 
TATATTTCTA CTGGAAAGTC TGAGAATGCT GAGATAGGAA ACACTTACCG GCACCCATAT 
CCTTCCAATA CTGAGCCAGA AAGTCTAAAG GAGCCACTAG ATCCTTTTCT GCATCACAAG 
TCAGCAGTTG CTCCAAAGAC ATATAGAGCA GAAACACTTC TACCACATAT GCCTCCTCAA 
GTTTATGGTT CAAGATGTGA TACTCCATCC TCAACATTTT ATGGCACCTA CATAAATCAG 
GCCAAACACC CCCGCACCAG GAACAGACCT GATTATATGT CCTCTATGAG CCCAGGTCTC 
CGTAGCTACT CAGAAGACAC ATCTTCCTAC CCTACTATTC GAAGGGTACA ATCTCTGCAT 
GTGCCTATGC AACCTCCGCC ACCTCCTATC CGCACTGTTC CAATTTCAAG AACAGAGGTT 
CCCCCAGATG ATGATCCAGC ATACTGCCCA AGACCAGTTT ATCAATACAA ACAATGCCCC 
CAATTTAACT CTCAGTCAGA TTACCATGTT ACCCAGCTGC AGCCTTACTT TGAAAATGGA 
CGAGTTCATT ATCGCTACAG TCCCTATTCA GGGGGCCCAA GTTATTTTTC AGCAGATAGC 
AATTTCTATG ATATGGACCC CTACAGCACC TTACGTCTGC GACAACTTCC TCTCTACCCA 
AGCCGGGACT TTGCTTCTTA CACCACAAGA TTGCAGCCCA AAAACACATA TCGGTCACAA 
GGATTGCCCC CATATCCCAG AGGAACTCTA CGGGAACACA ATTTTATCAG TAGAGATGTT 
CCTCCTGCAC TACCACCTGA ACATCCACGA CCTCTTCACA TTTCTTGGGA CATGGAAGAC 
ATGGACAGAT ACAGACTGCA ATCCCTGCGA CGAGAGAACA GGGCACGTCA GAGAGCTAAA 
GGACCAGTAA TGTCTCAGTA TGATAATGTT ACACCAACCT TAGTGGAGGA TGTAGCAGGC 
TTAGATGTCA TACATCTCCG AAGCCGTTCT GATCCTGGTA AGACTCCTGG TCTTTTAAGT 
GTGGCTGAGA ACAAAGACGT CCGTTACCCA GGAAGGGCAG AAGGTGATGA GCATACAACA 
TATCCACCTC CTGTTTTTGG AAATGGCCAT CAAGATAAGC CATCTCTTCC TCAAAAGCAA 
AGTGGATCAA GCCGGGTTCG AATGCAACAT GAAATATCTA CTGAGCAGCG GACTCAACCT 
TCTCAAGATA CTTTGCATAG GCAACCCACA GAGGGACGAA ATGGTCCTCC AATTCCTCCT 
GTGGACTACA GCTCTAAAGG AATGCCAAAT CCCCCAGACC CTGCATCATA TCATTCCTCT 
GCAAACAAAT ATAACTTGAC ACAACAGGAA CCCATGCGAC TTATTCACAA AGAGCTTCGG 
CTCCCTGAGG ATATTGAGCA TTCACATACC AGGCCAGCTG ACAACCAACA CCGTGACTGT 
TACAGGGAGG AGCCAGCTCA GTTTCCCCTT ACTGCACCTC CTCCTAAACC AGAGAGGAGC 
CATAGCCTTC GAGGTCATAA TCCAGATGTT TTAGAAAGAG ACCCTAGTAT CTTCTACCCC 
TACCAAACGC TTCAAGCCAA GAGACACAGC ACAATTAATG CAGTGTCACA ATATGATAAT 
CTAGCTGATT ACCATTCTAT ACCCCATCAT CGAACCACTA ACCAGTCTAC TCCAAATGCC 
TTTAACCTCC CACATGGACG GACCTATGCT ACTGCCCTGG GCCAGGGTGC CTTTCTAGCT 
GCTGAACTCG GCTTGCAAAG GCCTGAGACA AAAATCCATG TGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916222.2
CDS	1225..7212
Translation	MEGGTDAAAFAAFPSIHAVPSTSGRMATGGKLDELERIMKSSLHLEEDDFVPELPRSIHPRERPDWEETISAMARGADIPVPGEIPTMPTEIPLRSCGSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGSIQLTLTEDQNEVSRNGCESKELVFLVQIACQGRSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLSELPRSDSVKDSPELVTQMLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINVPAIAAAHVIKRYNAQAPDELSFEVGDIVSVIDMPPKELTTWWRGKHGFQVGFFPSECVELINDKVPQSMTNSVPKPVSPAHGMRPASWSYFPPYLEMDSLRPGTSCCSEVLSHSQLSSVSRKHGKLITFLRTFMKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSDAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_004916165.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916165.4

ORF Insert Sequence:

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGTCTCC 
CCTGCGCATG GAATGAGGCC GGCCTCCTGG AGCTACTTTC CCCCCTATTT GGAGATGGAT 
AGTTTAAGGC CTGGAACATC GTGCTGTTCT GAGGTATTAA GTCACTCGCA GCTAAGCTCA 
GTGTCCCGGA AGCACGGCAA GCTCATCACA TTTCTAAGAA CCTTCATGAA GTCCAGACCC 
AGCAAACAGA AACTGAAGCA GAGAGGGATC CTGAGGGAGC GAGTGTTTGG TTGTGACCTT 
GGGGAACATC TGCTTAACTC TGGTCAGGAT GTCCCCCAAG TCCTCAGGAG CTGCACAGAG 
TTTATAGAAA AACATGGCAT TGTTGATGGA ATCTACCGTC TGTCTGGGAT TGCATCTAAC 
ATCCAGAAAC TACGTCATGA ATTTGACTCT GAACAGATTC CAGACCTGAC TAAAGATGTG 
TACATCCAGG ACATTCACTG TGTTGGCTCT CTCTGTAAAC TCTACTTCCG GGAACTCCCA 
AACCCTCTGC TCACATATCA GTTGTATGAA AAGTTCTCTG ATGCTGTGTC AGCGGCTACT 
GATGAAGAGA GACTGGTGAA AATTCATGAT GTTATTCAAC AGCTTCCCCC ACCTCATTAC 
AGAACACTGG AGTTCCTTAT GCGCCACTTA TCAAGACTTG CGACATACTG CTCTATCACC 
AATATGCACA CTAAAAATTT GGCAATTGTC TGGGCCCCTA ACCTGCTCCG GTCAAAACAG 
ATTGAGTCTG CATGTTTTAG CGGTACAGCA GCTTTCATGG AAGTGCGCAT ACAGTCTGTG 
GTAGTTGAAT TTATTCTAAA CCATGTGGAG GTTCTCTTTA GTCCTAAACT TAGTTCTGTG 
ATAAGGGATG GTGCTGGTCA CACTTCTTTG TCTCGGCCCA AGTCACTGTT GGTGTCATCT 
CCATCTACAA AACTACTAAG CCTAGAGGAG GCTCAAGCAA GGACACAGGC CCAAATCAAT 
TCCCCTGTTG TGGCTGACAG CAAGTACATT GAGGTGGGAG AGGGACCAGC TGCTTTGCAG 
GGCAGATTCC ACACTGTAAT TGACTTCCCT TCTGAAAGGA AAAGACCGCC AACCAAAATG 
AAAAAATCTC CTGTTGGAAG CTGGCGTTCT TTTTTTAGCA TAGGCAAATC ATCATCTTCA 
GCCTCCATGT CTAAGCGGAA ACTTCAGCGT AATCCAAGTG AGCCTTTGGA AATGAAATCC 
ATGGCATTTG CAGGTGGAAG AGATAGCAGC ACCATGCGCT CAGCTAAAAG CGAGGAGTCT 
CTCAGTTCCT TGCATGCAGC AGATGGAGAG TCGAAGCTAT TTCGTCCACG TCGTCCTCGT 
TCCAGCAGCG ATGCTCTGTC TGCTTCATAT AATGGGGACC TTCTGGATAG CTGCAACCGT 
TGCAACTCCT ATGATAATCT GCCCCGAGAC CAAGACAGTG ATGGAGATGA GGGTTTGATT 
CATGTGCCTG CTATGCTGTC AGGTCGCTCT CCTGAGGATG AAGAACTCAG CCCACCAGAC 
ATTGGTATGG CCAGTTTGGA CTTTGACCCA ATGTCTTTTC AGTGCAGCCC TCCCCAGCTA 
GAATCTGAAG GACCAGACAT TTTTTTTCAG CTTGACATTT GTGCTAGCAA TGGCAAGGAT 
AAGGTGCAAA GTGATGCACA AAACCTAGGC GGTGTCTCCA GCTCCGAACC TCACTCTCCT 
TTCCAAGAGA AAGTCGTTAG TCCATTTCTA TCGCCTGACC GCAGTTCTAG TACAGATAAA 
GTATCCAAAA CTACATCATT TGCAGAAAAA GTTGTGCAGG CCTTGTCACC TAAAGTGGGG 
CGCAAAGCAG TTCGATCTCC TCCATTAACC ATCTCAGAGC CTGTCACCAT CTCTCTCCCT 
AGTCGGGTAT CGGAAATGAT TGGAAGTATG CAAGGCAATA CTGCTGCTTC CCAGTCTTTA 
ATTTGGTACA GAGAAAACAG TTTAAATTCT AGAGAAACTG AAATGAATTA TGTTTGTAAA 
TCGGTGGATG CCAACCTTCC ATCCTTATTG CCAGCAACAG ATCCATGGTC TACAGTTACT 
CCTGTAATTA CTCAATGGAA CCCAGATGGA TTACAGAGAG AAGACAAAGC AGGACCATTT 
ACTCATGGCT TTCCCCAGCC AAATAGTGAC TTGGAACAAG TAGAAAACTT CAACCTGGAA 
CTTAGCACAA CAAATGTTGC TGCAGCATAT CAGAAAGAAG ATACAGAATT GTCGAGAGCA 
AATCAGAACA AATCCCACTC TTCTGTTTCT CCCCCTCAAG GAGCTTCTGC TTCAGAATCT 
CCACAGGAGC TAGCCCCTGT CAGTAGTGTT TCTATCATTC CACCTCCACC TCCTCCAAAG 
AATCCTGCAC GCATGCTGGC ATTAGCACTG GCTGAATCTG CTCAACAGGC ATCTGCTAAT 
AAAAGACCTC TTTATATGCA GTTCATGGAT CTACCCCCCC ATCCTCCTCC ACCATCAGTA 
CTAGAGGACA AACCACTTCT TGAGTTATCT CCAAAAATGT CTCCAGAGCA CCTTGTATCT 
GGTCCTGCAA CACCAGCGAC CAGTCCTCCA GTCATATCCA ATAAAACATC TCCAGCTACT 
CCACCAAGCA CAACTTCATC ATTTTCTATC ACAACAATTC ATCACAGTCC TGTGAAAAAC 
CATAGTCCTC TGGCAGGACA GATGCCTACA GCAGTCACTC CTGGACTCAG TCCTTCATCA 
AACCAGGTTC CTGTATCCTT TCTATCTGAT GAAATGCTAC CTTCTGAAAC AAATAAAATG 
AATTCTGTCA TACTGAGTGA AGCATTTACA ACAGGAATTC CTGTTACTCC ACCATCTGAA 
AAACCCAAGG AAATTTCTAG ACCACCACAT CTTCACCAGC GTTCTGAATC CTTTCCTTCA 
CATCCTGCAT ATAGCAATGC AAAGCCCACT CCACCAGTTA GAACAATGGA TAGTCGACTG 
GCAACTGCCA TGCATTCCAA CTTCAATGAT GCCATCACTG CTAATAACTA CCACTCCTTC 
CTGAACACCA TGATGCTTCC TTCTTCTCTA GAGGATGTGC TACCCAGACA CAACTATTCT 
CATTCTTCTC ATCTCAAGAC AGAAAATATA GATGAACAGG GAGTTAATTA CAGACAACCT 
TATTTATCCA ACACCAAGCA AGAGGACCCA GCTGATGTGG GAGATTTATA CAGACAGCAA 
TATATTTCTA CTGGAAAGTC TGAGAATGCT GAGATAGGAA ACACTTACCG GCACCCATAT 
CCTTCCAATA CTGAGCCAGA AAGTCTAAAG GAGCCACTAG ATCCTTTTCT GCATCACAAG 
TCAGCAGTTG CTCCAAAGAC ATATAGAGCA GAAACACTTC TACCACATAT GCCTCCTCAA 
GTTTATGGTT CAAGATGTGA TACTCCATCC TCAACATTTT ATGGCACCTA CATAAATCAG 
GCCAAACACC CCCGCACCAG GAACAGACCT GATTATATGT CCTCTATGAG CCCAGGTCTC 
CGTAGCTACT CAGAAGACAC ATCTTCCTAC CCTACTATTC GAAGGGTACA ATCTCTGCAT 
GTGCCTATGC AACCTCCGCC ACCTCCTATC CGCACTGTTC CAATTTCAAG AACAGAGGTT 
CCCCCAGATG ATGATCCAGC ATACTGCCCA AGACCAGTTT ATCAATACAA ACAATGCCCC 
CAATTTAACT CTCAGTCAGA TTACCATGTT ACCCAGCTGC AGCCTTACTT TGAAAATGGA 
CGAGTTCATT ATCGCTACAG TCCCTATTCA GGGGGCCCAA GTTATTTTTC AGCAGATAGC 
AATTTCTATG ATATGGACCC CTACAGCACC TTACGTCTGC GACAACTTCC TCTCTACCCA 
AGCCGGGACT TTGCTTCTTA CACCACAAGA TTGCAGCCCA AAAACACATA TCGGTCACAA 
GGATTGCCCC CATATCCCAG AGGAACTCTA CGGGAACACA ATTTTATCAG TAGAGATGTT 
CCTCCTGCAC TACCACCTGA ACATCCACGA CCTCTTCACA TTTCTTGGGA CATGGAAGAC 
ATGGACAGAT ACAGACTGCA ATCCCTGCGA CGAGAGAACA GGGCACGTCA GAGAGCTAAA 
GGACCAGTAA TGTCTCAGTA TGATAATGTT ACACCAACCT TAGTGGAGGA TGTAGCAGGC 
TTAGATGTCA TACATCTCCG AAGCCGTTCT GATCCTGGTA AGACTCCTGG TCTTTTAAGT 
GTGGCTGAGA ACAAAGACGT CCGTTACCCA GGAAGGGCAG AAGGTGATGA GCATACAACA 
TATCCACCTC CTGTTTTTGG AAATGGCCAT CAAGATAAGC CATCTCTTCC TCAAAAGCAA 
AGTGGATCAA GCCGGGTTCG AATGCAACAT GAAATATCTA CTGAGCAGCG GACTCAACCT 
TCTCAAGATA CTTTGCATAG GCAACCCACA GAGGGACGAA ATGGTCCTCC AATTCCTCCT 
GTGGACTACA GCTCTAAAGG AATGCCAAAT CCCCCAGACC CTGCATCATA TCATTCCTCT 
GCAAACAAAT ATAACTTGAC ACAACAGGAA CCCATGCGAC TTATTCACAA AGAGCTTCGG 
CTCCCTGAGG ATATTGAGCA TTCACATACC AGGCCAGCTG ACAACCAACA CCGTGACTGT 
TACAGGGAGG AGCCAGCTCA GTTTCCCCTT ACTGCACCTC CTCCTAAACC AGAGAGGAGC 
CATAGCCTTC GAGGTCATAA TCCAGATGTT TTAGAAAGAG ACCCTAGTAT CTTCTACCCC 
TACCAAACGC TTCAAGCCAA GAGACACAGC ACAATTAATG CAGTGTCACA ATATGATAAT 
CTAGCTGATT ACCATTCTAT ACCCCATCAT CGAACCACTA ACCAGTCTAC TCCAAATGCC 
TTTAACCTCC CACATGGACG GACCTATGCT ACTGCCCTGG GCCAGGGTGC CTTTCTAGCT 
GCTGAACTCG GCTTGCAAAG GCCTGAGACA AAAATCCATG TGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_004916164.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916221.2 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X2, mRNA.

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CloneID	OXa61961
Clone ID Related Accession (Same CDS sequence)	XM_004916165.4 , XM_004916164.4 , XM_031905576.1
Accession Version	XM_004916164.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916164.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGTCTCC 
CCTGCGCATG GAATGAGGCC GGCCTCCTGG AGCTACTTTC CCCCCTATTT GGAGATGGAT 
AGTTTAAGGC CTGGAACATC GTGCTGTTCT GAGGTATTAA GTCACTCGCA GCTAAGCTCA 
GTGTCCCGGA AGCACGGCAA GCTCATCACA TTTCTAAGAA CCTTCATGAA GTCCAGACCC 
AGCAAACAGA AACTGAAGCA GAGAGGGATC CTGAGGGAGC GAGTGTTTGG TTGTGACCTT 
GGGGAACATC TGCTTAACTC TGGTCAGGAT GTCCCCCAAG TCCTCAGGAG CTGCACAGAG 
TTTATAGAAA AACATGGCAT TGTTGATGGA ATCTACCGTC TGTCTGGGAT TGCATCTAAC 
ATCCAGAAAC TACGTCATGA ATTTGACTCT GAACAGATTC CAGACCTGAC TAAAGATGTG 
TACATCCAGG ACATTCACTG TGTTGGCTCT CTCTGTAAAC TCTACTTCCG GGAACTCCCA 
AACCCTCTGC TCACATATCA GTTGTATGAA AAGTTCTCTG ATGCTGTGTC AGCGGCTACT 
GATGAAGAGA GACTGGTGAA AATTCATGAT GTTATTCAAC AGCTTCCCCC ACCTCATTAC 
AGAACACTGG AGTTCCTTAT GCGCCACTTA TCAAGACTTG CGACATACTG CTCTATCACC 
AATATGCACA CTAAAAATTT GGCAATTGTC TGGGCCCCTA ACCTGCTCCG GTCAAAACAG 
ATTGAGTCTG CATGTTTTAG CGGTACAGCA GCTTTCATGG AAGTGCGCAT ACAGTCTGTG 
GTAGTTGAAT TTATTCTAAA CCATGTGGAG GTTCTCTTTA GTCCTAAACT TAGTTCTGTG 
ATAAGGGATG GTGCTGGTCA CACTTCTTTG TCTCGGCCCA AGTCACTGTT GGTGTCATCT 
CCATCTACAA AACTACTAAG CCTAGAGGAG GCTCAAGCAA GGACACAGGC CCAAATCAAT 
TCCCCTGTTG TGGCTGACAG CAAGTACATT GAGGTGGGAG AGGGACCAGC TGCTTTGCAG 
GGCAGATTCC ACACTGTAAT TGACTTCCCT TCTGAAAGGA AAAGACCGCC AACCAAAATG 
AAAAAATCTC CTGTTGGAAG CTGGCGTTCT TTTTTTAGCA TAGGCAAATC ATCATCTTCA 
GCCTCCATGT CTAAGCGGAA ACTTCAGCGT AATCCAAGTG AGCCTTTGGA AATGAAATCC 
ATGGCATTTG CAGGTGGAAG AGATAGCAGC ACCATGCGCT CAGCTAAAAG CGAGGAGTCT 
CTCAGTTCCT TGCATGCAGC AGATGGAGAG TCGAAGCTAT TTCGTCCACG TCGTCCTCGT 
TCCAGCAGCG ATGCTCTGTC TGCTTCATAT AATGGGGACC TTCTGGATAG CTGCAACCGT 
TGCAACTCCT ATGATAATCT GCCCCGAGAC CAAGACAGTG ATGGAGATGA GGGTTTGATT 
CATGTGCCTG CTATGCTGTC AGGTCGCTCT CCTGAGGATG AAGAACTCAG CCCACCAGAC 
ATTGGTATGG CCAGTTTGGA CTTTGACCCA ATGTCTTTTC AGTGCAGCCC TCCCCAGCTA 
GAATCTGAAG GACCAGACAT TTTTTTTCAG CTTGACATTT GTGCTAGCAA TGGCAAGGAT 
AAGGTGCAAA GTGATGCACA AAACCTAGGC GGTGTCTCCA GCTCCGAACC TCACTCTCCT 
TTCCAAGAGA AAGTCGTTAG TCCATTTCTA TCGCCTGACC GCAGTTCTAG TACAGATAAA 
GTATCCAAAA CTACATCATT TGCAGAAAAA GTTGTGCAGG CCTTGTCACC TAAAGTGGGG 
CGCAAAGCAG TTCGATCTCC TCCATTAACC ATCTCAGAGC CTGTCACCAT CTCTCTCCCT 
AGTCGGGTAT CGGAAATGAT TGGAAGTATG CAAGGCAATA CTGCTGCTTC CCAGTCTTTA 
ATTTGGTACA GAGAAAACAG TTTAAATTCT AGAGAAACTG AAATGAATTA TGTTTGTAAA 
TCGGTGGATG CCAACCTTCC ATCCTTATTG CCAGCAACAG ATCCATGGTC TACAGTTACT 
CCTGTAATTA CTCAATGGAA CCCAGATGGA TTACAGAGAG AAGACAAAGC AGGACCATTT 
ACTCATGGCT TTCCCCAGCC AAATAGTGAC TTGGAACAAG TAGAAAACTT CAACCTGGAA 
CTTAGCACAA CAAATGTTGC TGCAGCATAT CAGAAAGAAG ATACAGAATT GTCGAGAGCA 
AATCAGAACA AATCCCACTC TTCTGTTTCT CCCCCTCAAG GAGCTTCTGC TTCAGAATCT 
CCACAGGAGC TAGCCCCTGT CAGTAGTGTT TCTATCATTC CACCTCCACC TCCTCCAAAG 
AATCCTGCAC GCATGCTGGC ATTAGCACTG GCTGAATCTG CTCAACAGGC ATCTGCTAAT 
AAAAGACCTC TTTATATGCA GTTCATGGAT CTACCCCCCC ATCCTCCTCC ACCATCAGTA 
CTAGAGGACA AACCACTTCT TGAGTTATCT CCAAAAATGT CTCCAGAGCA CCTTGTATCT 
GGTCCTGCAA CACCAGCGAC CAGTCCTCCA GTCATATCCA ATAAAACATC TCCAGCTACT 
CCACCAAGCA CAACTTCATC ATTTTCTATC ACAACAATTC ATCACAGTCC TGTGAAAAAC 
CATAGTCCTC TGGCAGGACA GATGCCTACA GCAGTCACTC CTGGACTCAG TCCTTCATCA 
AACCAGGTTC CTGTATCCTT TCTATCTGAT GAAATGCTAC CTTCTGAAAC AAATAAAATG 
AATTCTGTCA TACTGAGTGA AGCATTTACA ACAGGAATTC CTGTTACTCC ACCATCTGAA 
AAACCCAAGG AAATTTCTAG ACCACCACAT CTTCACCAGC GTTCTGAATC CTTTCCTTCA 
CATCCTGCAT ATAGCAATGC AAAGCCCACT CCACCAGTTA GAACAATGGA TAGTCGACTG 
GCAACTGCCA TGCATTCCAA CTTCAATGAT GCCATCACTG CTAATAACTA CCACTCCTTC 
CTGAACACCA TGATGCTTCC TTCTTCTCTA GAGGATGTGC TACCCAGACA CAACTATTCT 
CATTCTTCTC ATCTCAAGAC AGAAAATATA GATGAACAGG GAGTTAATTA CAGACAACCT 
TATTTATCCA ACACCAAGCA AGAGGACCCA GCTGATGTGG GAGATTTATA CAGACAGCAA 
TATATTTCTA CTGGAAAGTC TGAGAATGCT GAGATAGGAA ACACTTACCG GCACCCATAT 
CCTTCCAATA CTGAGCCAGA AAGTCTAAAG GAGCCACTAG ATCCTTTTCT GCATCACAAG 
TCAGCAGTTG CTCCAAAGAC ATATAGAGCA GAAACACTTC TACCACATAT GCCTCCTCAA 
GTTTATGGTT CAAGATGTGA TACTCCATCC TCAACATTTT ATGGCACCTA CATAAATCAG 
GCCAAACACC CCCGCACCAG GAACAGACCT GATTATATGT CCTCTATGAG CCCAGGTCTC 
CGTAGCTACT CAGAAGACAC ATCTTCCTAC CCTACTATTC GAAGGGTACA ATCTCTGCAT 
GTGCCTATGC AACCTCCGCC ACCTCCTATC CGCACTGTTC CAATTTCAAG AACAGAGGTT 
CCCCCAGATG ATGATCCAGC ATACTGCCCA AGACCAGTTT ATCAATACAA ACAATGCCCC 
CAATTTAACT CTCAGTCAGA TTACCATGTT ACCCAGCTGC AGCCTTACTT TGAAAATGGA 
CGAGTTCATT ATCGCTACAG TCCCTATTCA GGGGGCCCAA GTTATTTTTC AGCAGATAGC 
AATTTCTATG ATATGGACCC CTACAGCACC TTACGTCTGC GACAACTTCC TCTCTACCCA 
AGCCGGGACT TTGCTTCTTA CACCACAAGA TTGCAGCCCA AAAACACATA TCGGTCACAA 
GGATTGCCCC CATATCCCAG AGGAACTCTA CGGGAACACA ATTTTATCAG TAGAGATGTT 
CCTCCTGCAC TACCACCTGA ACATCCACGA CCTCTTCACA TTTCTTGGGA CATGGAAGAC 
ATGGACAGAT ACAGACTGCA ATCCCTGCGA CGAGAGAACA GGGCACGTCA GAGAGCTAAA 
GGACCAGTAA TGTCTCAGTA TGATAATGTT ACACCAACCT TAGTGGAGGA TGTAGCAGGC 
TTAGATGTCA TACATCTCCG AAGCCGTTCT GATCCTGGTA AGACTCCTGG TCTTTTAAGT 
GTGGCTGAGA ACAAAGACGT CCGTTACCCA GGAAGGGCAG AAGGTGATGA GCATACAACA 
TATCCACCTC CTGTTTTTGG AAATGGCCAT CAAGATAAGC CATCTCTTCC TCAAAAGCAA 
AGTGGATCAA GCCGGGTTCG AATGCAACAT GAAATATCTA CTGAGCAGCG GACTCAACCT 
TCTCAAGATA CTTTGCATAG GCAACCCACA GAGGGACGAA ATGGTCCTCC AATTCCTCCT 
GTGGACTACA GCTCTAAAGG AATGCCAAAT CCCCCAGACC CTGCATCATA TCATTCCTCT 
GCAAACAAAT ATAACTTGAC ACAACAGGAA CCCATGCGAC TTATTCACAA AGAGCTTCGG 
CTCCCTGAGG ATATTGAGCA TTCACATACC AGGCCAGCTG ACAACCAACA CCGTGACTGT 
TACAGGGAGG AGCCAGCTCA GTTTCCCCTT ACTGCACCTC CTCCTAAACC AGAGAGGAGC 
CATAGCCTTC GAGGTCATAA TCCAGATGTT TTAGAAAGAG ACCCTAGTAT CTTCTACCCC 
TACCAAACGC TTCAAGCCAA GAGACACAGC ACAATTAATG CAGTGTCACA ATATGATAAT 
CTAGCTGATT ACCATTCTAT ACCCCATCAT CGAACCACTA ACCAGTCTAC TCCAAATGCC 
TTTAACCTCC CACATGGACG GACCTATGCT ACTGCCCTGG GCCAGGGTGC CTTTCTAGCT 
GCTGAACTCG GCTTGCAAAG GCCTGAGACA AAAATCCATG TGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916221.2
CDS	1265..7252
Translation	MEGGTDAAAFAAFPSIHAVPSTSGRMATGGKLDELERIMKSSLHLEEDDFVPELPRSIHPRERPDWEETISAMARGADIPVPGEIPTMPTEIPLRSCGSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGSIQLTLTEDQNEVSRNGCESKELVFLVQIACQGRSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLSELPRSDSVKDSPELVTQMLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINVPAIAAAHVIKRYNAQAPDELSFEVGDIVSVIDMPPKELTTWWRGKHGFQVGFFPSECVELINDKVPQSMTNSVPKPVSPAHGMRPASWSYFPPYLEMDSLRPGTSCCSEVLSHSQLSSVSRKHGKLITFLRTFMKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSDAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_004916164.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916164.4

ORF Insert Sequence:

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGTCTCC 
CCTGCGCATG GAATGAGGCC GGCCTCCTGG AGCTACTTTC CCCCCTATTT GGAGATGGAT 
AGTTTAAGGC CTGGAACATC GTGCTGTTCT GAGGTATTAA GTCACTCGCA GCTAAGCTCA 
GTGTCCCGGA AGCACGGCAA GCTCATCACA TTTCTAAGAA CCTTCATGAA GTCCAGACCC 
AGCAAACAGA AACTGAAGCA GAGAGGGATC CTGAGGGAGC GAGTGTTTGG TTGTGACCTT 
GGGGAACATC TGCTTAACTC TGGTCAGGAT GTCCCCCAAG TCCTCAGGAG CTGCACAGAG 
TTTATAGAAA AACATGGCAT TGTTGATGGA ATCTACCGTC TGTCTGGGAT TGCATCTAAC 
ATCCAGAAAC TACGTCATGA ATTTGACTCT GAACAGATTC CAGACCTGAC TAAAGATGTG 
TACATCCAGG ACATTCACTG TGTTGGCTCT CTCTGTAAAC TCTACTTCCG GGAACTCCCA 
AACCCTCTGC TCACATATCA GTTGTATGAA AAGTTCTCTG ATGCTGTGTC AGCGGCTACT 
GATGAAGAGA GACTGGTGAA AATTCATGAT GTTATTCAAC AGCTTCCCCC ACCTCATTAC 
AGAACACTGG AGTTCCTTAT GCGCCACTTA TCAAGACTTG CGACATACTG CTCTATCACC 
AATATGCACA CTAAAAATTT GGCAATTGTC TGGGCCCCTA ACCTGCTCCG GTCAAAACAG 
ATTGAGTCTG CATGTTTTAG CGGTACAGCA GCTTTCATGG AAGTGCGCAT ACAGTCTGTG 
GTAGTTGAAT TTATTCTAAA CCATGTGGAG GTTCTCTTTA GTCCTAAACT TAGTTCTGTG 
ATAAGGGATG GTGCTGGTCA CACTTCTTTG TCTCGGCCCA AGTCACTGTT GGTGTCATCT 
CCATCTACAA AACTACTAAG CCTAGAGGAG GCTCAAGCAA GGACACAGGC CCAAATCAAT 
TCCCCTGTTG TGGCTGACAG CAAGTACATT GAGGTGGGAG AGGGACCAGC TGCTTTGCAG 
GGCAGATTCC ACACTGTAAT TGACTTCCCT TCTGAAAGGA AAAGACCGCC AACCAAAATG 
AAAAAATCTC CTGTTGGAAG CTGGCGTTCT TTTTTTAGCA TAGGCAAATC ATCATCTTCA 
GCCTCCATGT CTAAGCGGAA ACTTCAGCGT AATCCAAGTG AGCCTTTGGA AATGAAATCC 
ATGGCATTTG CAGGTGGAAG AGATAGCAGC ACCATGCGCT CAGCTAAAAG CGAGGAGTCT 
CTCAGTTCCT TGCATGCAGC AGATGGAGAG TCGAAGCTAT TTCGTCCACG TCGTCCTCGT 
TCCAGCAGCG ATGCTCTGTC TGCTTCATAT AATGGGGACC TTCTGGATAG CTGCAACCGT 
TGCAACTCCT ATGATAATCT GCCCCGAGAC CAAGACAGTG ATGGAGATGA GGGTTTGATT 
CATGTGCCTG CTATGCTGTC AGGTCGCTCT CCTGAGGATG AAGAACTCAG CCCACCAGAC 
ATTGGTATGG CCAGTTTGGA CTTTGACCCA ATGTCTTTTC AGTGCAGCCC TCCCCAGCTA 
GAATCTGAAG GACCAGACAT TTTTTTTCAG CTTGACATTT GTGCTAGCAA TGGCAAGGAT 
AAGGTGCAAA GTGATGCACA AAACCTAGGC GGTGTCTCCA GCTCCGAACC TCACTCTCCT 
TTCCAAGAGA AAGTCGTTAG TCCATTTCTA TCGCCTGACC GCAGTTCTAG TACAGATAAA 
GTATCCAAAA CTACATCATT TGCAGAAAAA GTTGTGCAGG CCTTGTCACC TAAAGTGGGG 
CGCAAAGCAG TTCGATCTCC TCCATTAACC ATCTCAGAGC CTGTCACCAT CTCTCTCCCT 
AGTCGGGTAT CGGAAATGAT TGGAAGTATG CAAGGCAATA CTGCTGCTTC CCAGTCTTTA 
ATTTGGTACA GAGAAAACAG TTTAAATTCT AGAGAAACTG AAATGAATTA TGTTTGTAAA 
TCGGTGGATG CCAACCTTCC ATCCTTATTG CCAGCAACAG ATCCATGGTC TACAGTTACT 
CCTGTAATTA CTCAATGGAA CCCAGATGGA TTACAGAGAG AAGACAAAGC AGGACCATTT 
ACTCATGGCT TTCCCCAGCC AAATAGTGAC TTGGAACAAG TAGAAAACTT CAACCTGGAA 
CTTAGCACAA CAAATGTTGC TGCAGCATAT CAGAAAGAAG ATACAGAATT GTCGAGAGCA 
AATCAGAACA AATCCCACTC TTCTGTTTCT CCCCCTCAAG GAGCTTCTGC TTCAGAATCT 
CCACAGGAGC TAGCCCCTGT CAGTAGTGTT TCTATCATTC CACCTCCACC TCCTCCAAAG 
AATCCTGCAC GCATGCTGGC ATTAGCACTG GCTGAATCTG CTCAACAGGC ATCTGCTAAT 
AAAAGACCTC TTTATATGCA GTTCATGGAT CTACCCCCCC ATCCTCCTCC ACCATCAGTA 
CTAGAGGACA AACCACTTCT TGAGTTATCT CCAAAAATGT CTCCAGAGCA CCTTGTATCT 
GGTCCTGCAA CACCAGCGAC CAGTCCTCCA GTCATATCCA ATAAAACATC TCCAGCTACT 
CCACCAAGCA CAACTTCATC ATTTTCTATC ACAACAATTC ATCACAGTCC TGTGAAAAAC 
CATAGTCCTC TGGCAGGACA GATGCCTACA GCAGTCACTC CTGGACTCAG TCCTTCATCA 
AACCAGGTTC CTGTATCCTT TCTATCTGAT GAAATGCTAC CTTCTGAAAC AAATAAAATG 
AATTCTGTCA TACTGAGTGA AGCATTTACA ACAGGAATTC CTGTTACTCC ACCATCTGAA 
AAACCCAAGG AAATTTCTAG ACCACCACAT CTTCACCAGC GTTCTGAATC CTTTCCTTCA 
CATCCTGCAT ATAGCAATGC AAAGCCCACT CCACCAGTTA GAACAATGGA TAGTCGACTG 
GCAACTGCCA TGCATTCCAA CTTCAATGAT GCCATCACTG CTAATAACTA CCACTCCTTC 
CTGAACACCA TGATGCTTCC TTCTTCTCTA GAGGATGTGC TACCCAGACA CAACTATTCT 
CATTCTTCTC ATCTCAAGAC AGAAAATATA GATGAACAGG GAGTTAATTA CAGACAACCT 
TATTTATCCA ACACCAAGCA AGAGGACCCA GCTGATGTGG GAGATTTATA CAGACAGCAA 
TATATTTCTA CTGGAAAGTC TGAGAATGCT GAGATAGGAA ACACTTACCG GCACCCATAT 
CCTTCCAATA CTGAGCCAGA AAGTCTAAAG GAGCCACTAG ATCCTTTTCT GCATCACAAG 
TCAGCAGTTG CTCCAAAGAC ATATAGAGCA GAAACACTTC TACCACATAT GCCTCCTCAA 
GTTTATGGTT CAAGATGTGA TACTCCATCC TCAACATTTT ATGGCACCTA CATAAATCAG 
GCCAAACACC CCCGCACCAG GAACAGACCT GATTATATGT CCTCTATGAG CCCAGGTCTC 
CGTAGCTACT CAGAAGACAC ATCTTCCTAC CCTACTATTC GAAGGGTACA ATCTCTGCAT 
GTGCCTATGC AACCTCCGCC ACCTCCTATC CGCACTGTTC CAATTTCAAG AACAGAGGTT 
CCCCCAGATG ATGATCCAGC ATACTGCCCA AGACCAGTTT ATCAATACAA ACAATGCCCC 
CAATTTAACT CTCAGTCAGA TTACCATGTT ACCCAGCTGC AGCCTTACTT TGAAAATGGA 
CGAGTTCATT ATCGCTACAG TCCCTATTCA GGGGGCCCAA GTTATTTTTC AGCAGATAGC 
AATTTCTATG ATATGGACCC CTACAGCACC TTACGTCTGC GACAACTTCC TCTCTACCCA 
AGCCGGGACT TTGCTTCTTA CACCACAAGA TTGCAGCCCA AAAACACATA TCGGTCACAA 
GGATTGCCCC CATATCCCAG AGGAACTCTA CGGGAACACA ATTTTATCAG TAGAGATGTT 
CCTCCTGCAC TACCACCTGA ACATCCACGA CCTCTTCACA TTTCTTGGGA CATGGAAGAC 
ATGGACAGAT ACAGACTGCA ATCCCTGCGA CGAGAGAACA GGGCACGTCA GAGAGCTAAA 
GGACCAGTAA TGTCTCAGTA TGATAATGTT ACACCAACCT TAGTGGAGGA TGTAGCAGGC 
TTAGATGTCA TACATCTCCG AAGCCGTTCT GATCCTGGTA AGACTCCTGG TCTTTTAAGT 
GTGGCTGAGA ACAAAGACGT CCGTTACCCA GGAAGGGCAG AAGGTGATGA GCATACAACA 
TATCCACCTC CTGTTTTTGG AAATGGCCAT CAAGATAAGC CATCTCTTCC TCAAAAGCAA 
AGTGGATCAA GCCGGGTTCG AATGCAACAT GAAATATCTA CTGAGCAGCG GACTCAACCT 
TCTCAAGATA CTTTGCATAG GCAACCCACA GAGGGACGAA ATGGTCCTCC AATTCCTCCT 
GTGGACTACA GCTCTAAAGG AATGCCAAAT CCCCCAGACC CTGCATCATA TCATTCCTCT 
GCAAACAAAT ATAACTTGAC ACAACAGGAA CCCATGCGAC TTATTCACAA AGAGCTTCGG 
CTCCCTGAGG ATATTGAGCA TTCACATACC AGGCCAGCTG ACAACCAACA CCGTGACTGT 
TACAGGGAGG AGCCAGCTCA GTTTCCCCTT ACTGCACCTC CTCCTAAACC AGAGAGGAGC 
CATAGCCTTC GAGGTCATAA TCCAGATGTT TTAGAAAGAG ACCCTAGTAT CTTCTACCCC 
TACCAAACGC TTCAAGCCAA GAGACACAGC ACAATTAATG CAGTGTCACA ATATGATAAT 
CTAGCTGATT ACCATTCTAT ACCCCATCAT CGAACCACTA ACCAGTCTAC TCCAAATGCC 
TTTAACCTCC CACATGGACG GACCTATGCT ACTGCCCTGG GCCAGGGTGC CTTTCTAGCT 
GCTGAACTCG GCTTGCAAAG GCCTGAGACA AAAATCCATG TGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_031905576.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761436.1 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X1, mRNA.

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CloneID	OXa61961
Clone ID Related Accession (Same CDS sequence)	XM_004916165.4 , XM_004916164.4 , XM_031905576.1
Accession Version	XM_031905576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGTCTCC 
CCTGCGCATG GAATGAGGCC GGCCTCCTGG AGCTACTTTC CCCCCTATTT GGAGATGGAT 
AGTTTAAGGC CTGGAACATC GTGCTGTTCT GAGGTATTAA GTCACTCGCA GCTAAGCTCA 
GTGTCCCGGA AGCACGGCAA GCTCATCACA TTTCTAAGAA CCTTCATGAA GTCCAGACCC 
AGCAAACAGA AACTGAAGCA GAGAGGGATC CTGAGGGAGC GAGTGTTTGG TTGTGACCTT 
GGGGAACATC TGCTTAACTC TGGTCAGGAT GTCCCCCAAG TCCTCAGGAG CTGCACAGAG 
TTTATAGAAA AACATGGCAT TGTTGATGGA ATCTACCGTC TGTCTGGGAT TGCATCTAAC 
ATCCAGAAAC TACGTCATGA ATTTGACTCT GAACAGATTC CAGACCTGAC TAAAGATGTG 
TACATCCAGG ACATTCACTG TGTTGGCTCT CTCTGTAAAC TCTACTTCCG GGAACTCCCA 
AACCCTCTGC TCACATATCA GTTGTATGAA AAGTTCTCTG ATGCTGTGTC AGCGGCTACT 
GATGAAGAGA GACTGGTGAA AATTCATGAT GTTATTCAAC AGCTTCCCCC ACCTCATTAC 
AGAACACTGG AGTTCCTTAT GCGCCACTTA TCAAGACTTG CGACATACTG CTCTATCACC 
AATATGCACA CTAAAAATTT GGCAATTGTC TGGGCCCCTA ACCTGCTCCG GTCAAAACAG 
ATTGAGTCTG CATGTTTTAG CGGTACAGCA GCTTTCATGG AAGTGCGCAT ACAGTCTGTG 
GTAGTTGAAT TTATTCTAAA CCATGTGGAG GTTCTCTTTA GTCCTAAACT TAGTTCTGTG 
ATAAGGGATG GTGCTGGTCA CACTTCTTTG TCTCGGCCCA AGTCACTGTT GGTGTCATCT 
CCATCTACAA AACTACTAAG CCTAGAGGAG GCTCAAGCAA GGACACAGGC CCAAATCAAT 
TCCCCTGTTG TGGCTGACAG CAAGTACATT GAGGTGGGAG AGGGACCAGC TGCTTTGCAG 
GGCAGATTCC ACACTGTAAT TGACTTCCCT TCTGAAAGGA AAAGACCGCC AACCAAAATG 
AAAAAATCTC CTGTTGGAAG CTGGCGTTCT TTTTTTAGCA TAGGCAAATC ATCATCTTCA 
GCCTCCATGT CTAAGCGGAA ACTTCAGCGT AATCCAAGTG AGCCTTTGGA AATGAAATCC 
ATGGCATTTG CAGGTGGAAG AGATAGCAGC ACCATGCGCT CAGCTAAAAG CGAGGAGTCT 
CTCAGTTCCT TGCATGCAGC AGATGGAGAG TCGAAGCTAT TTCGTCCACG TCGTCCTCGT 
TCCAGCAGCG ATGCTCTGTC TGCTTCATAT AATGGGGACC TTCTGGATAG CTGCAACCGT 
TGCAACTCCT ATGATAATCT GCCCCGAGAC CAAGACAGTG ATGGAGATGA GGGTTTGATT 
CATGTGCCTG CTATGCTGTC AGGTCGCTCT CCTGAGGATG AAGAACTCAG CCCACCAGAC 
ATTGGTATGG CCAGTTTGGA CTTTGACCCA ATGTCTTTTC AGTGCAGCCC TCCCCAGCTA 
GAATCTGAAG GACCAGACAT TTTTTTTCAG CTTGACATTT GTGCTAGCAA TGGCAAGGAT 
AAGGTGCAAA GTGATGCACA AAACCTAGGC GGTGTCTCCA GCTCCGAACC TCACTCTCCT 
TTCCAAGAGA AAGTCGTTAG TCCATTTCTA TCGCCTGACC GCAGTTCTAG TACAGATAAA 
GTATCCAAAA CTACATCATT TGCAGAAAAA GTTGTGCAGG CCTTGTCACC TAAAGTGGGG 
CGCAAAGCAG TTCGATCTCC TCCATTAACC ATCTCAGAGC CTGTCACCAT CTCTCTCCCT 
AGTCGGGTAT CGGAAATGAT TGGAAGTATG CAAGGCAATA CTGCTGCTTC CCAGTCTTTA 
ATTTGGTACA GAGAAAACAG TTTAAATTCT AGAGAAACTG AAATGAATTA TGTTTGTAAA 
TCGGTGGATG CCAACCTTCC ATCCTTATTG CCAGCAACAG ATCCATGGTC TACAGTTACT 
CCTGTAATTA CTCAATGGAA CCCAGATGGA TTACAGAGAG AAGACAAAGC AGGACCATTT 
ACTCATGGCT TTCCCCAGCC AAATAGTGAC TTGGAACAAG TAGAAAACTT CAACCTGGAA 
CTTAGCACAA CAAATGTTGC TGCAGCATAT CAGAAAGAAG ATACAGAATT GTCGAGAGCA 
AATCAGAACA AATCCCACTC TTCTGTTTCT CCCCCTCAAG GAGCTTCTGC TTCAGAATCT 
CCACAGGAGC TAGCCCCTGT CAGTAGTGTT TCTATCATTC CACCTCCACC TCCTCCAAAG 
AATCCTGCAC GCATGCTGGC ATTAGCACTG GCTGAATCTG CTCAACAGGC ATCTGCTAAT 
AAAAGACCTC TTTATATGCA GTTCATGGAT CTACCCCCCC ATCCTCCTCC ACCATCAGTA 
CTAGAGGACA AACCACTTCT TGAGTTATCT CCAAAAATGT CTCCAGAGCA CCTTGTATCT 
GGTCCTGCAA CACCAGCGAC CAGTCCTCCA GTCATATCCA ATAAAACATC TCCAGCTACT 
CCACCAAGCA CAACTTCATC ATTTTCTATC ACAACAATTC ATCACAGTCC TGTGAAAAAC 
CATAGTCCTC TGGCAGGACA GATGCCTACA GCAGTCACTC CTGGACTCAG TCCTTCATCA 
AACCAGGTTC CTGTATCCTT TCTATCTGAT GAAATGCTAC CTTCTGAAAC AAATAAAATG 
AATTCTGTCA TACTGAGTGA AGCATTTACA ACAGGAATTC CTGTTACTCC ACCATCTGAA 
AAACCCAAGG AAATTTCTAG ACCACCACAT CTTCACCAGC GTTCTGAATC CTTTCCTTCA 
CATCCTGCAT ATAGCAATGC AAAGCCCACT CCACCAGTTA GAACAATGGA TAGTCGACTG 
GCAACTGCCA TGCATTCCAA CTTCAATGAT GCCATCACTG CTAATAACTA CCACTCCTTC 
CTGAACACCA TGATGCTTCC TTCTTCTCTA GAGGATGTGC TACCCAGACA CAACTATTCT 
CATTCTTCTC ATCTCAAGAC AGAAAATATA GATGAACAGG GAGTTAATTA CAGACAACCT 
TATTTATCCA ACACCAAGCA AGAGGACCCA GCTGATGTGG GAGATTTATA CAGACAGCAA 
TATATTTCTA CTGGAAAGTC TGAGAATGCT GAGATAGGAA ACACTTACCG GCACCCATAT 
CCTTCCAATA CTGAGCCAGA AAGTCTAAAG GAGCCACTAG ATCCTTTTCT GCATCACAAG 
TCAGCAGTTG CTCCAAAGAC ATATAGAGCA GAAACACTTC TACCACATAT GCCTCCTCAA 
GTTTATGGTT CAAGATGTGA TACTCCATCC TCAACATTTT ATGGCACCTA CATAAATCAG 
GCCAAACACC CCCGCACCAG GAACAGACCT GATTATATGT CCTCTATGAG CCCAGGTCTC 
CGTAGCTACT CAGAAGACAC ATCTTCCTAC CCTACTATTC GAAGGGTACA ATCTCTGCAT 
GTGCCTATGC AACCTCCGCC ACCTCCTATC CGCACTGTTC CAATTTCAAG AACAGAGGTT 
CCCCCAGATG ATGATCCAGC ATACTGCCCA AGACCAGTTT ATCAATACAA ACAATGCCCC 
CAATTTAACT CTCAGTCAGA TTACCATGTT ACCCAGCTGC AGCCTTACTT TGAAAATGGA 
CGAGTTCATT ATCGCTACAG TCCCTATTCA GGGGGCCCAA GTTATTTTTC AGCAGATAGC 
AATTTCTATG ATATGGACCC CTACAGCACC TTACGTCTGC GACAACTTCC TCTCTACCCA 
AGCCGGGACT TTGCTTCTTA CACCACAAGA TTGCAGCCCA AAAACACATA TCGGTCACAA 
GGATTGCCCC CATATCCCAG AGGAACTCTA CGGGAACACA ATTTTATCAG TAGAGATGTT 
CCTCCTGCAC TACCACCTGA ACATCCACGA CCTCTTCACA TTTCTTGGGA CATGGAAGAC 
ATGGACAGAT ACAGACTGCA ATCCCTGCGA CGAGAGAACA GGGCACGTCA GAGAGCTAAA 
GGACCAGTAA TGTCTCAGTA TGATAATGTT ACACCAACCT TAGTGGAGGA TGTAGCAGGC 
TTAGATGTCA TACATCTCCG AAGCCGTTCT GATCCTGGTA AGACTCCTGG TCTTTTAAGT 
GTGGCTGAGA ACAAAGACGT CCGTTACCCA GGAAGGGCAG AAGGTGATGA GCATACAACA 
TATCCACCTC CTGTTTTTGG AAATGGCCAT CAAGATAAGC CATCTCTTCC TCAAAAGCAA 
AGTGGATCAA GCCGGGTTCG AATGCAACAT GAAATATCTA CTGAGCAGCG GACTCAACCT 
TCTCAAGATA CTTTGCATAG GCAACCCACA GAGGGACGAA ATGGTCCTCC AATTCCTCCT 
GTGGACTACA GCTCTAAAGG AATGCCAAAT CCCCCAGACC CTGCATCATA TCATTCCTCT 
GCAAACAAAT ATAACTTGAC ACAACAGGAA CCCATGCGAC TTATTCACAA AGAGCTTCGG 
CTCCCTGAGG ATATTGAGCA TTCACATACC AGGCCAGCTG ACAACCAACA CCGTGACTGT 
TACAGGGAGG AGCCAGCTCA GTTTCCCCTT ACTGCACCTC CTCCTAAACC AGAGAGGAGC 
CATAGCCTTC GAGGTCATAA TCCAGATGTT TTAGAAAGAG ACCCTAGTAT CTTCTACCCC 
TACCAAACGC TTCAAGCCAA GAGACACAGC ACAATTAATG CAGTGTCACA ATATGATAAT 
CTAGCTGATT ACCATTCTAT ACCCCATCAT CGAACCACTA ACCAGTCTAC TCCAAATGCC 
TTTAACCTCC CACATGGACG GACCTATGCT ACTGCCCTGG GCCAGGGTGC CTTTCTAGCT 
GCTGAACTCG GCTTGCAAAG GCCTGAGACA AAAATCCATG TGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761436.1
CDS	1380..7367
Translation	MEGGTDAAAFAAFPSIHAVPSTSGRMATGGKLDELERIMKSSLHLEEDDFVPELPRSIHPRERPDWEETISAMARGADIPVPGEIPTMPTEIPLRSCGSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGSIQLTLTEDQNEVSRNGCESKELVFLVQIACQGRSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLSELPRSDSVKDSPELVTQMLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINVPAIAAAHVIKRYNAQAPDELSFEVGDIVSVIDMPPKELTTWWRGKHGFQVGFFPSECVELINDKVPQSMTNSVPKPVSPAHGMRPASWSYFPPYLEMDSLRPGTSCCSEVLSHSQLSSVSRKHGKLITFLRTFMKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSDAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_031905576.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905576.1

ORF Insert Sequence:

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGTCTCC 
CCTGCGCATG GAATGAGGCC GGCCTCCTGG AGCTACTTTC CCCCCTATTT GGAGATGGAT 
AGTTTAAGGC CTGGAACATC GTGCTGTTCT GAGGTATTAA GTCACTCGCA GCTAAGCTCA 
GTGTCCCGGA AGCACGGCAA GCTCATCACA TTTCTAAGAA CCTTCATGAA GTCCAGACCC 
AGCAAACAGA AACTGAAGCA GAGAGGGATC CTGAGGGAGC GAGTGTTTGG TTGTGACCTT 
GGGGAACATC TGCTTAACTC TGGTCAGGAT GTCCCCCAAG TCCTCAGGAG CTGCACAGAG 
TTTATAGAAA AACATGGCAT TGTTGATGGA ATCTACCGTC TGTCTGGGAT TGCATCTAAC 
ATCCAGAAAC TACGTCATGA ATTTGACTCT GAACAGATTC CAGACCTGAC TAAAGATGTG 
TACATCCAGG ACATTCACTG TGTTGGCTCT CTCTGTAAAC TCTACTTCCG GGAACTCCCA 
AACCCTCTGC TCACATATCA GTTGTATGAA AAGTTCTCTG ATGCTGTGTC AGCGGCTACT 
GATGAAGAGA GACTGGTGAA AATTCATGAT GTTATTCAAC AGCTTCCCCC ACCTCATTAC 
AGAACACTGG AGTTCCTTAT GCGCCACTTA TCAAGACTTG CGACATACTG CTCTATCACC 
AATATGCACA CTAAAAATTT GGCAATTGTC TGGGCCCCTA ACCTGCTCCG GTCAAAACAG 
ATTGAGTCTG CATGTTTTAG CGGTACAGCA GCTTTCATGG AAGTGCGCAT ACAGTCTGTG 
GTAGTTGAAT TTATTCTAAA CCATGTGGAG GTTCTCTTTA GTCCTAAACT TAGTTCTGTG 
ATAAGGGATG GTGCTGGTCA CACTTCTTTG TCTCGGCCCA AGTCACTGTT GGTGTCATCT 
CCATCTACAA AACTACTAAG CCTAGAGGAG GCTCAAGCAA GGACACAGGC CCAAATCAAT 
TCCCCTGTTG TGGCTGACAG CAAGTACATT GAGGTGGGAG AGGGACCAGC TGCTTTGCAG 
GGCAGATTCC ACACTGTAAT TGACTTCCCT TCTGAAAGGA AAAGACCGCC AACCAAAATG 
AAAAAATCTC CTGTTGGAAG CTGGCGTTCT TTTTTTAGCA TAGGCAAATC ATCATCTTCA 
GCCTCCATGT CTAAGCGGAA ACTTCAGCGT AATCCAAGTG AGCCTTTGGA AATGAAATCC 
ATGGCATTTG CAGGTGGAAG AGATAGCAGC ACCATGCGCT CAGCTAAAAG CGAGGAGTCT 
CTCAGTTCCT TGCATGCAGC AGATGGAGAG TCGAAGCTAT TTCGTCCACG TCGTCCTCGT 
TCCAGCAGCG ATGCTCTGTC TGCTTCATAT AATGGGGACC TTCTGGATAG CTGCAACCGT 
TGCAACTCCT ATGATAATCT GCCCCGAGAC CAAGACAGTG ATGGAGATGA GGGTTTGATT 
CATGTGCCTG CTATGCTGTC AGGTCGCTCT CCTGAGGATG AAGAACTCAG CCCACCAGAC 
ATTGGTATGG CCAGTTTGGA CTTTGACCCA ATGTCTTTTC AGTGCAGCCC TCCCCAGCTA 
GAATCTGAAG GACCAGACAT TTTTTTTCAG CTTGACATTT GTGCTAGCAA TGGCAAGGAT 
AAGGTGCAAA GTGATGCACA AAACCTAGGC GGTGTCTCCA GCTCCGAACC TCACTCTCCT 
TTCCAAGAGA AAGTCGTTAG TCCATTTCTA TCGCCTGACC GCAGTTCTAG TACAGATAAA 
GTATCCAAAA CTACATCATT TGCAGAAAAA GTTGTGCAGG CCTTGTCACC TAAAGTGGGG 
CGCAAAGCAG TTCGATCTCC TCCATTAACC ATCTCAGAGC CTGTCACCAT CTCTCTCCCT 
AGTCGGGTAT CGGAAATGAT TGGAAGTATG CAAGGCAATA CTGCTGCTTC CCAGTCTTTA 
ATTTGGTACA GAGAAAACAG TTTAAATTCT AGAGAAACTG AAATGAATTA TGTTTGTAAA 
TCGGTGGATG CCAACCTTCC ATCCTTATTG CCAGCAACAG ATCCATGGTC TACAGTTACT 
CCTGTAATTA CTCAATGGAA CCCAGATGGA TTACAGAGAG AAGACAAAGC AGGACCATTT 
ACTCATGGCT TTCCCCAGCC AAATAGTGAC TTGGAACAAG TAGAAAACTT CAACCTGGAA 
CTTAGCACAA CAAATGTTGC TGCAGCATAT CAGAAAGAAG ATACAGAATT GTCGAGAGCA 
AATCAGAACA AATCCCACTC TTCTGTTTCT CCCCCTCAAG GAGCTTCTGC TTCAGAATCT 
CCACAGGAGC TAGCCCCTGT CAGTAGTGTT TCTATCATTC CACCTCCACC TCCTCCAAAG 
AATCCTGCAC GCATGCTGGC ATTAGCACTG GCTGAATCTG CTCAACAGGC ATCTGCTAAT 
AAAAGACCTC TTTATATGCA GTTCATGGAT CTACCCCCCC ATCCTCCTCC ACCATCAGTA 
CTAGAGGACA AACCACTTCT TGAGTTATCT CCAAAAATGT CTCCAGAGCA CCTTGTATCT 
GGTCCTGCAA CACCAGCGAC CAGTCCTCCA GTCATATCCA ATAAAACATC TCCAGCTACT 
CCACCAAGCA CAACTTCATC ATTTTCTATC ACAACAATTC ATCACAGTCC TGTGAAAAAC 
CATAGTCCTC TGGCAGGACA GATGCCTACA GCAGTCACTC CTGGACTCAG TCCTTCATCA 
AACCAGGTTC CTGTATCCTT TCTATCTGAT GAAATGCTAC CTTCTGAAAC AAATAAAATG 
AATTCTGTCA TACTGAGTGA AGCATTTACA ACAGGAATTC CTGTTACTCC ACCATCTGAA 
AAACCCAAGG AAATTTCTAG ACCACCACAT CTTCACCAGC GTTCTGAATC CTTTCCTTCA 
CATCCTGCAT ATAGCAATGC AAAGCCCACT CCACCAGTTA GAACAATGGA TAGTCGACTG 
GCAACTGCCA TGCATTCCAA CTTCAATGAT GCCATCACTG CTAATAACTA CCACTCCTTC 
CTGAACACCA TGATGCTTCC TTCTTCTCTA GAGGATGTGC TACCCAGACA CAACTATTCT 
CATTCTTCTC ATCTCAAGAC AGAAAATATA GATGAACAGG GAGTTAATTA CAGACAACCT 
TATTTATCCA ACACCAAGCA AGAGGACCCA GCTGATGTGG GAGATTTATA CAGACAGCAA 
TATATTTCTA CTGGAAAGTC TGAGAATGCT GAGATAGGAA ACACTTACCG GCACCCATAT 
CCTTCCAATA CTGAGCCAGA AAGTCTAAAG GAGCCACTAG ATCCTTTTCT GCATCACAAG 
TCAGCAGTTG CTCCAAAGAC ATATAGAGCA GAAACACTTC TACCACATAT GCCTCCTCAA 
GTTTATGGTT CAAGATGTGA TACTCCATCC TCAACATTTT ATGGCACCTA CATAAATCAG 
GCCAAACACC CCCGCACCAG GAACAGACCT GATTATATGT CCTCTATGAG CCCAGGTCTC 
CGTAGCTACT CAGAAGACAC ATCTTCCTAC CCTACTATTC GAAGGGTACA ATCTCTGCAT 
GTGCCTATGC AACCTCCGCC ACCTCCTATC CGCACTGTTC CAATTTCAAG AACAGAGGTT 
CCCCCAGATG ATGATCCAGC ATACTGCCCA AGACCAGTTT ATCAATACAA ACAATGCCCC 
CAATTTAACT CTCAGTCAGA TTACCATGTT ACCCAGCTGC AGCCTTACTT TGAAAATGGA 
CGAGTTCATT ATCGCTACAG TCCCTATTCA GGGGGCCCAA GTTATTTTTC AGCAGATAGC 
AATTTCTATG ATATGGACCC CTACAGCACC TTACGTCTGC GACAACTTCC TCTCTACCCA 
AGCCGGGACT TTGCTTCTTA CACCACAAGA TTGCAGCCCA AAAACACATA TCGGTCACAA 
GGATTGCCCC CATATCCCAG AGGAACTCTA CGGGAACACA ATTTTATCAG TAGAGATGTT 
CCTCCTGCAC TACCACCTGA ACATCCACGA CCTCTTCACA TTTCTTGGGA CATGGAAGAC 
ATGGACAGAT ACAGACTGCA ATCCCTGCGA CGAGAGAACA GGGCACGTCA GAGAGCTAAA 
GGACCAGTAA TGTCTCAGTA TGATAATGTT ACACCAACCT TAGTGGAGGA TGTAGCAGGC 
TTAGATGTCA TACATCTCCG AAGCCGTTCT GATCCTGGTA AGACTCCTGG TCTTTTAAGT 
GTGGCTGAGA ACAAAGACGT CCGTTACCCA GGAAGGGCAG AAGGTGATGA GCATACAACA 
TATCCACCTC CTGTTTTTGG AAATGGCCAT CAAGATAAGC CATCTCTTCC TCAAAAGCAA 
AGTGGATCAA GCCGGGTTCG AATGCAACAT GAAATATCTA CTGAGCAGCG GACTCAACCT 
TCTCAAGATA CTTTGCATAG GCAACCCACA GAGGGACGAA ATGGTCCTCC AATTCCTCCT 
GTGGACTACA GCTCTAAAGG AATGCCAAAT CCCCCAGACC CTGCATCATA TCATTCCTCT 
GCAAACAAAT ATAACTTGAC ACAACAGGAA CCCATGCGAC TTATTCACAA AGAGCTTCGG 
CTCCCTGAGG ATATTGAGCA TTCACATACC AGGCCAGCTG ACAACCAACA CCGTGACTGT 
TACAGGGAGG AGCCAGCTCA GTTTCCCCTT ACTGCACCTC CTCCTAAACC AGAGAGGAGC 
CATAGCCTTC GAGGTCATAA TCCAGATGTT TTAGAAAGAG ACCCTAGTAT CTTCTACCCC 
TACCAAACGC TTCAAGCCAA GAGACACAGC ACAATTAATG CAGTGTCACA ATATGATAAT 
CTAGCTGATT ACCATTCTAT ACCCCATCAT CGAACCACTA ACCAGTCTAC TCCAAATGCC 
TTTAACCTCC CACATGGACG GACCTATGCT ACTGCCCTGG GCCAGGGTGC CTTTCTAGCT 
GCTGAACTCG GCTTGCAAAG GCCTGAGACA AAAATCCATG TGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_018095928.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951417.2 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X4, mRNA.

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CloneID	OXa61962
Clone ID Related Accession (Same CDS sequence)	XM_018095928.2
Accession Version	XM_018095928.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018095928.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGATTTG 
GAGATGGATA GTTTAAGGCC TGGAACATCG TGCTGTTCTG AGGTATTAAG TCACTCGCAG 
CTAAGCTCAG TGTCCCGGAA GCACGGCAAG CTCATCACAT TTCTAAGAAC CTTCATGAAG 
TCCAGACCCA GCAAACAGAA ACTGAAGCAG AGAGGGATCC TGAGGGAGCG AGTGTTTGGT 
TGTGACCTTG GGGAACATCT GCTTAACTCT GGTCAGGATG TCCCCCAAGT CCTCAGGAGC 
TGCACAGAGT TTATAGAAAA ACATGGCATT GTTGATGGAA TCTACCGTCT GTCTGGGATT 
GCATCTAACA TCCAGAAACT ACGTCATGAA TTTGACTCTG AACAGATTCC AGACCTGACT 
AAAGATGTGT ACATCCAGGA CATTCACTGT GTTGGCTCTC TCTGTAAACT CTACTTCCGG 
GAACTCCCAA ACCCTCTGCT CACATATCAG TTGTATGAAA AGTTCTCTGA TGCTGTGTCA 
GCGGCTACTG ATGAAGAGAG ACTGGTGAAA ATTCATGATG TTATTCAACA GCTTCCCCCA 
CCTCATTACA GAACACTGGA GTTCCTTATG CGCCACTTAT CAAGACTTGC GACATACTGC 
TCTATCACCA ATATGCACAC TAAAAATTTG GCAATTGTCT GGGCCCCTAA CCTGCTCCGG 
TCAAAACAGA TTGAGTCTGC ATGTTTTAGC GGTACAGCAG CTTTCATGGA AGTGCGCATA 
CAGTCTGTGG TAGTTGAATT TATTCTAAAC CATGTGGAGG TTCTCTTTAG TCCTAAACTT 
AGTTCTGTGA TAAGGGATGG TGCTGGTCAC ACTTCTTTGT CTCGGCCCAA GTCACTGTTG 
GTGTCATCTC CATCTACAAA ACTACTAAGC CTAGAGGAGG CTCAAGCAAG GACACAGGCC 
CAAATCAATT CCCCTGTTGT GGCTGACAGC AAGTACATTG AGGTGGGAGA GGGACCAGCT 
GCTTTGCAGG GCAGATTCCA CACTGTAATT GACTTCCCTT CTGAAAGGAA AAGACCGCCA 
ACCAAAATGA AAAAATCTCC TGTTGGAAGC TGGCGTTCTT TTTTTAGCAT AGGCAAATCA 
TCATCTTCAG CCTCCATGTC TAAGCGGAAA CTTCAGCGTA ATCCAAGTGA GCCTTTGGAA 
ATGAAATCCA TGGCATTTGC AGGTGGAAGA GATAGCAGCA CCATGCGCTC AGCTAAAAGC 
GAGGAGTCTC TCAGTTCCTT GCATGCAGCA GATGGAGAGT CGAAGCTATT TCGTCCACGT 
CGTCCTCGTT CCAGCAGCGA TGCTCTGTCT GCTTCATATA ATGGGGACCT TCTGGATAGC 
TGCAACCGTT GCAACTCCTA TGATAATCTG CCCCGAGACC AAGACAGTGA TGGAGATGAG 
GGTTTGATTC ATGTGCCTGC TATGCTGTCA GGTCGCTCTC CTGAGGATGA AGAACTCAGC 
CCACCAGACA TTGGTATGGC CAGTTTGGAC TTTGACCCAA TGTCTTTTCA GTGCAGCCCT 
CCCCAGCTAG AATCTGAAGG ACCAGACATT TTTTTTCAGC TTGACATTTG TGCTAGCAAT 
GGCAAGGATA AGGTGCAAAG TGATGCACAA AACCTAGGCG GTGTCTCCAG CTCCGAACCT 
CACTCTCCTT TCCAAGAGAA AGTCGTTAGT CCATTTCTAT CGCCTGACCG CAGTTCTAGT 
ACAGATAAAG TATCCAAAAC TACATCATTT GCAGAAAAAG TTGTGCAGGC CTTGTCACCT 
AAAGTGGGGC GCAAAGCAGT TCGATCTCCT CCATTAACCA TCTCAGAGCC TGTCACCATC 
TCTCTCCCTA GTCGGGTATC GGAAATGATT GGAAGTATGC AAGGCAATAC TGCTGCTTCC 
CAGTCTTTAA TTTGGTACAG AGAAAACAGT TTAAATTCTA GAGAAACTGA AATGAATTAT 
GTTTGTAAAT CGGTGGATGC CAACCTTCCA TCCTTATTGC CAGCAACAGA TCCATGGTCT 
ACAGTTACTC CTGTAATTAC TCAATGGAAC CCAGATGGAT TACAGAGAGA AGACAAAGCA 
GGACCATTTA CTCATGGCTT TCCCCAGCCA AATAGTGACT TGGAACAAGT AGAAAACTTC 
AACCTGGAAC TTAGCACAAC AAATGTTGCT GCAGCATATC AGAAAGAAGA TACAGAATTG 
TCGAGAGCAA ATCAGAACAA ATCCCACTCT TCTGTTTCTC CCCCTCAAGG AGCTTCTGCT 
TCAGAATCTC CACAGGAGCT AGCCCCTGTC AGTAGTGTTT CTATCATTCC ACCTCCACCT 
CCTCCAAAGA ATCCTGCACG CATGCTGGCA TTAGCACTGG CTGAATCTGC TCAACAGGCA 
TCTGCTAATA AAAGACCTCT TTATATGCAG TTCATGGATC TACCCCCCCA TCCTCCTCCA 
CCATCAGTAC TAGAGGACAA ACCACTTCTT GAGTTATCTC CAAAAATGTC TCCAGAGCAC 
CTTGTATCTG GTCCTGCAAC ACCAGCGACC AGTCCTCCAG TCATATCCAA TAAAACATCT 
CCAGCTACTC CACCAAGCAC AACTTCATCA TTTTCTATCA CAACAATTCA TCACAGTCCT 
GTGAAAAACC ATAGTCCTCT GGCAGGACAG ATGCCTACAG CAGTCACTCC TGGACTCAGT 
CCTTCATCAA ACCAGGTTCC TGTATCCTTT CTATCTGATG AAATGCTACC TTCTGAAACA 
AATAAAATGA ATTCTGTCAT ACTGAGTGAA GCATTTACAA CAGGAATTCC TGTTACTCCA 
CCATCTGAAA AACCCAAGGA AATTTCTAGA CCACCACATC TTCACCAGCG TTCTGAATCC 
TTTCCTTCAC ATCCTGCATA TAGCAATGCA AAGCCCACTC CACCAGTTAG AACAATGGAT 
AGTCGACTGG CAACTGCCAT GCATTCCAAC TTCAATGATG CCATCACTGC TAATAACTAC 
CACTCCTTCC TGAACACCAT GATGCTTCCT TCTTCTCTAG AGGATGTGCT ACCCAGACAC 
AACTATTCTC ATTCTTCTCA TCTCAAGACA GAAAATATAG ATGAACAGGG AGTTAATTAC 
AGACAACCTT ATTTATCCAA CACCAAGCAA GAGGACCCAG CTGATGTGGG AGATTTATAC 
AGACAGCAAT ATATTTCTAC TGGAAAGTCT GAGAATGCTG AGATAGGAAA CACTTACCGG 
CACCCATATC CTTCCAATAC TGAGCCAGAA AGTCTAAAGG AGCCACTAGA TCCTTTTCTG 
CATCACAAGT CAGCAGTTGC TCCAAAGACA TATAGAGCAG AAACACTTCT ACCACATATG 
CCTCCTCAAG TTTATGGTTC AAGATGTGAT ACTCCATCCT CAACATTTTA TGGCACCTAC 
ATAAATCAGG CCAAACACCC CCGCACCAGG AACAGACCTG ATTATATGTC CTCTATGAGC 
CCAGGTCTCC GTAGCTACTC AGAAGACACA TCTTCCTACC CTACTATTCG AAGGGTACAA 
TCTCTGCATG TGCCTATGCA ACCTCCGCCA CCTCCTATCC GCACTGTTCC AATTTCAAGA 
ACAGAGGTTC CCCCAGATGA TGATCCAGCA TACTGCCCAA GACCAGTTTA TCAATACAAA 
CAATGCCCCC AATTTAACTC TCAGTCAGAT TACCATGTTA CCCAGCTGCA GCCTTACTTT 
GAAAATGGAC GAGTTCATTA TCGCTACAGT CCCTATTCAG GGGGCCCAAG TTATTTTTCA 
GCAGATAGCA ATTTCTATGA TATGGACCCC TACAGCACCT TACGTCTGCG ACAACTTCCT 
CTCTACCCAA GCCGGGACTT TGCTTCTTAC ACCACAAGAT TGCAGCCCAA AAACACATAT 
CGGTCACAAG GATTGCCCCC ATATCCCAGA GGAACTCTAC GGGAACACAA TTTTATCAGT 
AGAGATGTTC CTCCTGCACT ACCACCTGAA CATCCACGAC CTCTTCACAT TTCTTGGGAC 
ATGGAAGACA TGGACAGATA CAGACTGCAA TCCCTGCGAC GAGAGAACAG GGCACGTCAG 
AGAGCTAAAG GACCAGTAAT GTCTCAGTAT GATAATGTTA CACCAACCTT AGTGGAGGAT 
GTAGCAGGCT TAGATGTCAT ACATCTCCGA AGCCGTTCTG ATCCTGGTAA GACTCCTGGT 
CTTTTAAGTG TGGCTGAGAA CAAAGACGTC CGTTACCCAG GAAGGGCAGA AGGTGATGAG 
CATACAACAT ATCCACCTCC TGTTTTTGGA AATGGCCATC AAGATAAGCC ATCTCTTCCT 
CAAAAGCAAA GTGGATCAAG CCGGGTTCGA ATGCAACATG AAATATCTAC TGAGCAGCGG 
ACTCAACCTT CTCAAGATAC TTTGCATAGG CAACCCACAG AGGGACGAAA TGGTCCTCCA 
ATTCCTCCTG TGGACTACAG CTCTAAAGGA ATGCCAAATC CCCCAGACCC TGCATCATAT 
CATTCCTCTG CAAACAAATA TAACTTGACA CAACAGGAAC CCATGCGACT TATTCACAAA 
GAGCTTCGGC TCCCTGAGGA TATTGAGCAT TCACATACCA GGCCAGCTGA CAACCAACAC 
CGTGACTGTT ACAGGGAGGA GCCAGCTCAG TTTCCCCTTA CTGCACCTCC TCCTAAACCA 
GAGAGGAGCC ATAGCCTTCG AGGTCATAAT CCAGATGTTT TAGAAAGAGA CCCTAGTATC 
TTCTACCCCT ACCAAACGCT TCAAGCCAAG AGACACAGCA CAATTAATGC AGTGTCACAA 
TATGATAATC TAGCTGATTA CCATTCTATA CCCCATCATC GAACCACTAA CCAGTCTACT 
CCAAATGCCT TTAACCTCCC ACATGGACGG ACCTATGCTA CTGCCCTGGG CCAGGGTGCC 
TTTCTAGCTG CTGAACTCGG CTTGCAAAGG CCTGAGACAA AAATCCATGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951417.2
CDS	1380..7316
Translation	MEGGTDAAAFAAFPSIHAVPSTSGRMATGGKLDELERIMKSSLHLEEDDFVPELPRSIHPRERPDWEETISAMARGADIPVPGEIPTMPTEIPLRSCGSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGSIQLTLTEDQNEVSRNGCESKELVFLVQIACQGRSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLSELPRSDSVKDSPELVTQMLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINVPAIAAAHVIKRYNAQAPDELSFEVGDIVSVIDMPPKELTTWWRGKHGFQVGFFPSECVELINDKVPQSMTNSVPKPDLEMDSLRPGTSCCSEVLSHSQLSSVSRKHGKLITFLRTFMKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSDAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_018095928.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095928.2

ORF Insert Sequence:

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGATTTG 
GAGATGGATA GTTTAAGGCC TGGAACATCG TGCTGTTCTG AGGTATTAAG TCACTCGCAG 
CTAAGCTCAG TGTCCCGGAA GCACGGCAAG CTCATCACAT TTCTAAGAAC CTTCATGAAG 
TCCAGACCCA GCAAACAGAA ACTGAAGCAG AGAGGGATCC TGAGGGAGCG AGTGTTTGGT 
TGTGACCTTG GGGAACATCT GCTTAACTCT GGTCAGGATG TCCCCCAAGT CCTCAGGAGC 
TGCACAGAGT TTATAGAAAA ACATGGCATT GTTGATGGAA TCTACCGTCT GTCTGGGATT 
GCATCTAACA TCCAGAAACT ACGTCATGAA TTTGACTCTG AACAGATTCC AGACCTGACT 
AAAGATGTGT ACATCCAGGA CATTCACTGT GTTGGCTCTC TCTGTAAACT CTACTTCCGG 
GAACTCCCAA ACCCTCTGCT CACATATCAG TTGTATGAAA AGTTCTCTGA TGCTGTGTCA 
GCGGCTACTG ATGAAGAGAG ACTGGTGAAA ATTCATGATG TTATTCAACA GCTTCCCCCA 
CCTCATTACA GAACACTGGA GTTCCTTATG CGCCACTTAT CAAGACTTGC GACATACTGC 
TCTATCACCA ATATGCACAC TAAAAATTTG GCAATTGTCT GGGCCCCTAA CCTGCTCCGG 
TCAAAACAGA TTGAGTCTGC ATGTTTTAGC GGTACAGCAG CTTTCATGGA AGTGCGCATA 
CAGTCTGTGG TAGTTGAATT TATTCTAAAC CATGTGGAGG TTCTCTTTAG TCCTAAACTT 
AGTTCTGTGA TAAGGGATGG TGCTGGTCAC ACTTCTTTGT CTCGGCCCAA GTCACTGTTG 
GTGTCATCTC CATCTACAAA ACTACTAAGC CTAGAGGAGG CTCAAGCAAG GACACAGGCC 
CAAATCAATT CCCCTGTTGT GGCTGACAGC AAGTACATTG AGGTGGGAGA GGGACCAGCT 
GCTTTGCAGG GCAGATTCCA CACTGTAATT GACTTCCCTT CTGAAAGGAA AAGACCGCCA 
ACCAAAATGA AAAAATCTCC TGTTGGAAGC TGGCGTTCTT TTTTTAGCAT AGGCAAATCA 
TCATCTTCAG CCTCCATGTC TAAGCGGAAA CTTCAGCGTA ATCCAAGTGA GCCTTTGGAA 
ATGAAATCCA TGGCATTTGC AGGTGGAAGA GATAGCAGCA CCATGCGCTC AGCTAAAAGC 
GAGGAGTCTC TCAGTTCCTT GCATGCAGCA GATGGAGAGT CGAAGCTATT TCGTCCACGT 
CGTCCTCGTT CCAGCAGCGA TGCTCTGTCT GCTTCATATA ATGGGGACCT TCTGGATAGC 
TGCAACCGTT GCAACTCCTA TGATAATCTG CCCCGAGACC AAGACAGTGA TGGAGATGAG 
GGTTTGATTC ATGTGCCTGC TATGCTGTCA GGTCGCTCTC CTGAGGATGA AGAACTCAGC 
CCACCAGACA TTGGTATGGC CAGTTTGGAC TTTGACCCAA TGTCTTTTCA GTGCAGCCCT 
CCCCAGCTAG AATCTGAAGG ACCAGACATT TTTTTTCAGC TTGACATTTG TGCTAGCAAT 
GGCAAGGATA AGGTGCAAAG TGATGCACAA AACCTAGGCG GTGTCTCCAG CTCCGAACCT 
CACTCTCCTT TCCAAGAGAA AGTCGTTAGT CCATTTCTAT CGCCTGACCG CAGTTCTAGT 
ACAGATAAAG TATCCAAAAC TACATCATTT GCAGAAAAAG TTGTGCAGGC CTTGTCACCT 
AAAGTGGGGC GCAAAGCAGT TCGATCTCCT CCATTAACCA TCTCAGAGCC TGTCACCATC 
TCTCTCCCTA GTCGGGTATC GGAAATGATT GGAAGTATGC AAGGCAATAC TGCTGCTTCC 
CAGTCTTTAA TTTGGTACAG AGAAAACAGT TTAAATTCTA GAGAAACTGA AATGAATTAT 
GTTTGTAAAT CGGTGGATGC CAACCTTCCA TCCTTATTGC CAGCAACAGA TCCATGGTCT 
ACAGTTACTC CTGTAATTAC TCAATGGAAC CCAGATGGAT TACAGAGAGA AGACAAAGCA 
GGACCATTTA CTCATGGCTT TCCCCAGCCA AATAGTGACT TGGAACAAGT AGAAAACTTC 
AACCTGGAAC TTAGCACAAC AAATGTTGCT GCAGCATATC AGAAAGAAGA TACAGAATTG 
TCGAGAGCAA ATCAGAACAA ATCCCACTCT TCTGTTTCTC CCCCTCAAGG AGCTTCTGCT 
TCAGAATCTC CACAGGAGCT AGCCCCTGTC AGTAGTGTTT CTATCATTCC ACCTCCACCT 
CCTCCAAAGA ATCCTGCACG CATGCTGGCA TTAGCACTGG CTGAATCTGC TCAACAGGCA 
TCTGCTAATA AAAGACCTCT TTATATGCAG TTCATGGATC TACCCCCCCA TCCTCCTCCA 
CCATCAGTAC TAGAGGACAA ACCACTTCTT GAGTTATCTC CAAAAATGTC TCCAGAGCAC 
CTTGTATCTG GTCCTGCAAC ACCAGCGACC AGTCCTCCAG TCATATCCAA TAAAACATCT 
CCAGCTACTC CACCAAGCAC AACTTCATCA TTTTCTATCA CAACAATTCA TCACAGTCCT 
GTGAAAAACC ATAGTCCTCT GGCAGGACAG ATGCCTACAG CAGTCACTCC TGGACTCAGT 
CCTTCATCAA ACCAGGTTCC TGTATCCTTT CTATCTGATG AAATGCTACC TTCTGAAACA 
AATAAAATGA ATTCTGTCAT ACTGAGTGAA GCATTTACAA CAGGAATTCC TGTTACTCCA 
CCATCTGAAA AACCCAAGGA AATTTCTAGA CCACCACATC TTCACCAGCG TTCTGAATCC 
TTTCCTTCAC ATCCTGCATA TAGCAATGCA AAGCCCACTC CACCAGTTAG AACAATGGAT 
AGTCGACTGG CAACTGCCAT GCATTCCAAC TTCAATGATG CCATCACTGC TAATAACTAC 
CACTCCTTCC TGAACACCAT GATGCTTCCT TCTTCTCTAG AGGATGTGCT ACCCAGACAC 
AACTATTCTC ATTCTTCTCA TCTCAAGACA GAAAATATAG ATGAACAGGG AGTTAATTAC 
AGACAACCTT ATTTATCCAA CACCAAGCAA GAGGACCCAG CTGATGTGGG AGATTTATAC 
AGACAGCAAT ATATTTCTAC TGGAAAGTCT GAGAATGCTG AGATAGGAAA CACTTACCGG 
CACCCATATC CTTCCAATAC TGAGCCAGAA AGTCTAAAGG AGCCACTAGA TCCTTTTCTG 
CATCACAAGT CAGCAGTTGC TCCAAAGACA TATAGAGCAG AAACACTTCT ACCACATATG 
CCTCCTCAAG TTTATGGTTC AAGATGTGAT ACTCCATCCT CAACATTTTA TGGCACCTAC 
ATAAATCAGG CCAAACACCC CCGCACCAGG AACAGACCTG ATTATATGTC CTCTATGAGC 
CCAGGTCTCC GTAGCTACTC AGAAGACACA TCTTCCTACC CTACTATTCG AAGGGTACAA 
TCTCTGCATG TGCCTATGCA ACCTCCGCCA CCTCCTATCC GCACTGTTCC AATTTCAAGA 
ACAGAGGTTC CCCCAGATGA TGATCCAGCA TACTGCCCAA GACCAGTTTA TCAATACAAA 
CAATGCCCCC AATTTAACTC TCAGTCAGAT TACCATGTTA CCCAGCTGCA GCCTTACTTT 
GAAAATGGAC GAGTTCATTA TCGCTACAGT CCCTATTCAG GGGGCCCAAG TTATTTTTCA 
GCAGATAGCA ATTTCTATGA TATGGACCCC TACAGCACCT TACGTCTGCG ACAACTTCCT 
CTCTACCCAA GCCGGGACTT TGCTTCTTAC ACCACAAGAT TGCAGCCCAA AAACACATAT 
CGGTCACAAG GATTGCCCCC ATATCCCAGA GGAACTCTAC GGGAACACAA TTTTATCAGT 
AGAGATGTTC CTCCTGCACT ACCACCTGAA CATCCACGAC CTCTTCACAT TTCTTGGGAC 
ATGGAAGACA TGGACAGATA CAGACTGCAA TCCCTGCGAC GAGAGAACAG GGCACGTCAG 
AGAGCTAAAG GACCAGTAAT GTCTCAGTAT GATAATGTTA CACCAACCTT AGTGGAGGAT 
GTAGCAGGCT TAGATGTCAT ACATCTCCGA AGCCGTTCTG ATCCTGGTAA GACTCCTGGT 
CTTTTAAGTG TGGCTGAGAA CAAAGACGTC CGTTACCCAG GAAGGGCAGA AGGTGATGAG 
CATACAACAT ATCCACCTCC TGTTTTTGGA AATGGCCATC AAGATAAGCC ATCTCTTCCT 
CAAAAGCAAA GTGGATCAAG CCGGGTTCGA ATGCAACATG AAATATCTAC TGAGCAGCGG 
ACTCAACCTT CTCAAGATAC TTTGCATAGG CAACCCACAG AGGGACGAAA TGGTCCTCCA 
ATTCCTCCTG TGGACTACAG CTCTAAAGGA ATGCCAAATC CCCCAGACCC TGCATCATAT 
CATTCCTCTG CAAACAAATA TAACTTGACA CAACAGGAAC CCATGCGACT TATTCACAAA 
GAGCTTCGGC TCCCTGAGGA TATTGAGCAT TCACATACCA GGCCAGCTGA CAACCAACAC 
CGTGACTGTT ACAGGGAGGA GCCAGCTCAG TTTCCCCTTA CTGCACCTCC TCCTAAACCA 
GAGAGGAGCC ATAGCCTTCG AGGTCATAAT CCAGATGTTT TAGAAAGAGA CCCTAGTATC 
TTCTACCCCT ACCAAACGCT TCAAGCCAAG AGACACAGCA CAATTAATGC AGTGTCACAA 
TATGATAATC TAGCTGATTA CCATTCTATA CCCCATCATC GAACCACTAA CCAGTCTACT 
CCAAATGCCT TTAACCTCCC ACATGGACGG ACCTATGCTA CTGCCCTGGG CCAGGGTGCC 
TTTCTAGCTG CTGAACTCGG CTTGCAAAGG CCTGAGACAA AAATCCATGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_004916166.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916223.2 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X5, mRNA.

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CloneID	OXa61963
Clone ID Related Accession (Same CDS sequence)	XM_004916166.4
Accession Version	XM_004916166.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916166.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGTCTCC 
CCTGCGCATG GAATGAGGCC GGCCTCCTGG AGCTACTTTC CCCCCTTGTC CCGGAAGCAC 
GGCAAGCTCA TCACATTTCT AAGAACCTTC ATGAAGTCCA GACCCAGCAA ACAGAAACTG 
AAGCAGAGAG GGATCCTGAG GGAGCGAGTG TTTGGTTGTG ACCTTGGGGA ACATCTGCTT 
AACTCTGGTC AGGATGTCCC CCAAGTCCTC AGGAGCTGCA CAGAGTTTAT AGAAAAACAT 
GGCATTGTTG ATGGAATCTA CCGTCTGTCT GGGATTGCAT CTAACATCCA GAAACTACGT 
CATGAATTTG ACTCTGAACA GATTCCAGAC CTGACTAAAG ATGTGTACAT CCAGGACATT 
CACTGTGTTG GCTCTCTCTG TAAACTCTAC TTCCGGGAAC TCCCAAACCC TCTGCTCACA 
TATCAGTTGT ATGAAAAGTT CTCTGATGCT GTGTCAGCGG CTACTGATGA AGAGAGACTG 
GTGAAAATTC ATGATGTTAT TCAACAGCTT CCCCCACCTC ATTACAGAAC ACTGGAGTTC 
CTTATGCGCC ACTTATCAAG ACTTGCGACA TACTGCTCTA TCACCAATAT GCACACTAAA 
AATTTGGCAA TTGTCTGGGC CCCTAACCTG CTCCGGTCAA AACAGATTGA GTCTGCATGT 
TTTAGCGGTA CAGCAGCTTT CATGGAAGTG CGCATACAGT CTGTGGTAGT TGAATTTATT 
CTAAACCATG TGGAGGTTCT CTTTAGTCCT AAACTTAGTT CTGTGATAAG GGATGGTGCT 
GGTCACACTT CTTTGTCTCG GCCCAAGTCA CTGTTGGTGT CATCTCCATC TACAAAACTA 
CTAAGCCTAG AGGAGGCTCA AGCAAGGACA CAGGCCCAAA TCAATTCCCC TGTTGTGGCT 
GACAGCAAGT ACATTGAGGT GGGAGAGGGA CCAGCTGCTT TGCAGGGCAG ATTCCACACT 
GTAATTGACT TCCCTTCTGA AAGGAAAAGA CCGCCAACCA AAATGAAAAA ATCTCCTGTT 
GGAAGCTGGC GTTCTTTTTT TAGCATAGGC AAATCATCAT CTTCAGCCTC CATGTCTAAG 
CGGAAACTTC AGCGTAATCC AAGTGAGCCT TTGGAAATGA AATCCATGGC ATTTGCAGGT 
GGAAGAGATA GCAGCACCAT GCGCTCAGCT AAAAGCGAGG AGTCTCTCAG TTCCTTGCAT 
GCAGCAGATG GAGAGTCGAA GCTATTTCGT CCACGTCGTC CTCGTTCCAG CAGCGATGCT 
CTGTCTGCTT CATATAATGG GGACCTTCTG GATAGCTGCA ACCGTTGCAA CTCCTATGAT 
AATCTGCCCC GAGACCAAGA CAGTGATGGA GATGAGGGTT TGATTCATGT GCCTGCTATG 
CTGTCAGGTC GCTCTCCTGA GGATGAAGAA CTCAGCCCAC CAGACATTGG TATGGCCAGT 
TTGGACTTTG ACCCAATGTC TTTTCAGTGC AGCCCTCCCC AGCTAGAATC TGAAGGACCA 
GACATTTTTT TTCAGCTTGA CATTTGTGCT AGCAATGGCA AGGATAAGGT GCAAAGTGAT 
GCACAAAACC TAGGCGGTGT CTCCAGCTCC GAACCTCACT CTCCTTTCCA AGAGAAAGTC 
GTTAGTCCAT TTCTATCGCC TGACCGCAGT TCTAGTACAG ATAAAGTATC CAAAACTACA 
TCATTTGCAG AAAAAGTTGT GCAGGCCTTG TCACCTAAAG TGGGGCGCAA AGCAGTTCGA 
TCTCCTCCAT TAACCATCTC AGAGCCTGTC ACCATCTCTC TCCCTAGTCG GGTATCGGAA 
ATGATTGGAA GTATGCAAGG CAATACTGCT GCTTCCCAGT CTTTAATTTG GTACAGAGAA 
AACAGTTTAA ATTCTAGAGA AACTGAAATG AATTATGTTT GTAAATCGGT GGATGCCAAC 
CTTCCATCCT TATTGCCAGC AACAGATCCA TGGTCTACAG TTACTCCTGT AATTACTCAA 
TGGAACCCAG ATGGATTACA GAGAGAAGAC AAAGCAGGAC CATTTACTCA TGGCTTTCCC 
CAGCCAAATA GTGACTTGGA ACAAGTAGAA AACTTCAACC TGGAACTTAG CACAACAAAT 
GTTGCTGCAG CATATCAGAA AGAAGATACA GAATTGTCGA GAGCAAATCA GAACAAATCC 
CACTCTTCTG TTTCTCCCCC TCAAGGAGCT TCTGCTTCAG AATCTCCACA GGAGCTAGCC 
CCTGTCAGTA GTGTTTCTAT CATTCCACCT CCACCTCCTC CAAAGAATCC TGCACGCATG 
CTGGCATTAG CACTGGCTGA ATCTGCTCAA CAGGCATCTG CTAATAAAAG ACCTCTTTAT 
ATGCAGTTCA TGGATCTACC CCCCCATCCT CCTCCACCAT CAGTACTAGA GGACAAACCA 
CTTCTTGAGT TATCTCCAAA AATGTCTCCA GAGCACCTTG TATCTGGTCC TGCAACACCA 
GCGACCAGTC CTCCAGTCAT ATCCAATAAA ACATCTCCAG CTACTCCACC AAGCACAACT 
TCATCATTTT CTATCACAAC AATTCATCAC AGTCCTGTGA AAAACCATAG TCCTCTGGCA 
GGACAGATGC CTACAGCAGT CACTCCTGGA CTCAGTCCTT CATCAAACCA GGTTCCTGTA 
TCCTTTCTAT CTGATGAAAT GCTACCTTCT GAAACAAATA AAATGAATTC TGTCATACTG 
AGTGAAGCAT TTACAACAGG AATTCCTGTT ACTCCACCAT CTGAAAAACC CAAGGAAATT 
TCTAGACCAC CACATCTTCA CCAGCGTTCT GAATCCTTTC CTTCACATCC TGCATATAGC 
AATGCAAAGC CCACTCCACC AGTTAGAACA ATGGATAGTC GACTGGCAAC TGCCATGCAT 
TCCAACTTCA ATGATGCCAT CACTGCTAAT AACTACCACT CCTTCCTGAA CACCATGATG 
CTTCCTTCTT CTCTAGAGGA TGTGCTACCC AGACACAACT ATTCTCATTC TTCTCATCTC 
AAGACAGAAA ATATAGATGA ACAGGGAGTT AATTACAGAC AACCTTATTT ATCCAACACC 
AAGCAAGAGG ACCCAGCTGA TGTGGGAGAT TTATACAGAC AGCAATATAT TTCTACTGGA 
AAGTCTGAGA ATGCTGAGAT AGGAAACACT TACCGGCACC CATATCCTTC CAATACTGAG 
CCAGAAAGTC TAAAGGAGCC ACTAGATCCT TTTCTGCATC ACAAGTCAGC AGTTGCTCCA 
AAGACATATA GAGCAGAAAC ACTTCTACCA CATATGCCTC CTCAAGTTTA TGGTTCAAGA 
TGTGATACTC CATCCTCAAC ATTTTATGGC ACCTACATAA ATCAGGCCAA ACACCCCCGC 
ACCAGGAACA GACCTGATTA TATGTCCTCT ATGAGCCCAG GTCTCCGTAG CTACTCAGAA 
GACACATCTT CCTACCCTAC TATTCGAAGG GTACAATCTC TGCATGTGCC TATGCAACCT 
CCGCCACCTC CTATCCGCAC TGTTCCAATT TCAAGAACAG AGGTTCCCCC AGATGATGAT 
CCAGCATACT GCCCAAGACC AGTTTATCAA TACAAACAAT GCCCCCAATT TAACTCTCAG 
TCAGATTACC ATGTTACCCA GCTGCAGCCT TACTTTGAAA ATGGACGAGT TCATTATCGC 
TACAGTCCCT ATTCAGGGGG CCCAAGTTAT TTTTCAGCAG ATAGCAATTT CTATGATATG 
GACCCCTACA GCACCTTACG TCTGCGACAA CTTCCTCTCT ACCCAAGCCG GGACTTTGCT 
TCTTACACCA CAAGATTGCA GCCCAAAAAC ACATATCGGT CACAAGGATT GCCCCCATAT 
CCCAGAGGAA CTCTACGGGA ACACAATTTT ATCAGTAGAG ATGTTCCTCC TGCACTACCA 
CCTGAACATC CACGACCTCT TCACATTTCT TGGGACATGG AAGACATGGA CAGATACAGA 
CTGCAATCCC TGCGACGAGA GAACAGGGCA CGTCAGAGAG CTAAAGGACC AGTAATGTCT 
CAGTATGATA ATGTTACACC AACCTTAGTG GAGGATGTAG CAGGCTTAGA TGTCATACAT 
CTCCGAAGCC GTTCTGATCC TGGTAAGACT CCTGGTCTTT TAAGTGTGGC TGAGAACAAA 
GACGTCCGTT ACCCAGGAAG GGCAGAAGGT GATGAGCATA CAACATATCC ACCTCCTGTT 
TTTGGAAATG GCCATCAAGA TAAGCCATCT CTTCCTCAAA AGCAAAGTGG ATCAAGCCGG 
GTTCGAATGC AACATGAAAT ATCTACTGAG CAGCGGACTC AACCTTCTCA AGATACTTTG 
CATAGGCAAC CCACAGAGGG ACGAAATGGT CCTCCAATTC CTCCTGTGGA CTACAGCTCT 
AAAGGAATGC CAAATCCCCC AGACCCTGCA TCATATCATT CCTCTGCAAA CAAATATAAC 
TTGACACAAC AGGAACCCAT GCGACTTATT CACAAAGAGC TTCGGCTCCC TGAGGATATT 
GAGCATTCAC ATACCAGGCC AGCTGACAAC CAACACCGTG ACTGTTACAG GGAGGAGCCA 
GCTCAGTTTC CCCTTACTGC ACCTCCTCCT AAACCAGAGA GGAGCCATAG CCTTCGAGGT 
CATAATCCAG ATGTTTTAGA AAGAGACCCT AGTATCTTCT ACCCCTACCA AACGCTTCAA 
GCCAAGAGAC ACAGCACAAT TAATGCAGTG TCACAATATG ATAATCTAGC TGATTACCAT 
TCTATACCCC ATCATCGAAC CACTAACCAG TCTACTCCAA ATGCCTTTAA CCTCCCACAT 
GGACGGACCT ATGCTACTGC CCTGGGCCAG GGTGCCTTTC TAGCTGCTGA ACTCGGCTTG 
CAAAGGCCTG AGACAAAAAT CCATGTGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916223.2
CDS	1380..7292
Translation	MEGGTDAAAFAAFPSIHAVPSTSGRMATGGKLDELERIMKSSLHLEEDDFVPELPRSIHPRERPDWEETISAMARGADIPVPGEIPTMPTEIPLRSCGSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGSIQLTLTEDQNEVSRNGCESKELVFLVQIACQGRSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLSELPRSDSVKDSPELVTQMLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINVPAIAAAHVIKRYNAQAPDELSFEVGDIVSVIDMPPKELTTWWRGKHGFQVGFFPSECVELINDKVPQSMTNSVPKPVSPAHGMRPASWSYFPPLSRKHGKLITFLRTFMKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSDAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_004916166.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916166.4

ORF Insert Sequence:

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGTCTCC 
CCTGCGCATG GAATGAGGCC GGCCTCCTGG AGCTACTTTC CCCCCTTGTC CCGGAAGCAC 
GGCAAGCTCA TCACATTTCT AAGAACCTTC ATGAAGTCCA GACCCAGCAA ACAGAAACTG 
AAGCAGAGAG GGATCCTGAG GGAGCGAGTG TTTGGTTGTG ACCTTGGGGA ACATCTGCTT 
AACTCTGGTC AGGATGTCCC CCAAGTCCTC AGGAGCTGCA CAGAGTTTAT AGAAAAACAT 
GGCATTGTTG ATGGAATCTA CCGTCTGTCT GGGATTGCAT CTAACATCCA GAAACTACGT 
CATGAATTTG ACTCTGAACA GATTCCAGAC CTGACTAAAG ATGTGTACAT CCAGGACATT 
CACTGTGTTG GCTCTCTCTG TAAACTCTAC TTCCGGGAAC TCCCAAACCC TCTGCTCACA 
TATCAGTTGT ATGAAAAGTT CTCTGATGCT GTGTCAGCGG CTACTGATGA AGAGAGACTG 
GTGAAAATTC ATGATGTTAT TCAACAGCTT CCCCCACCTC ATTACAGAAC ACTGGAGTTC 
CTTATGCGCC ACTTATCAAG ACTTGCGACA TACTGCTCTA TCACCAATAT GCACACTAAA 
AATTTGGCAA TTGTCTGGGC CCCTAACCTG CTCCGGTCAA AACAGATTGA GTCTGCATGT 
TTTAGCGGTA CAGCAGCTTT CATGGAAGTG CGCATACAGT CTGTGGTAGT TGAATTTATT 
CTAAACCATG TGGAGGTTCT CTTTAGTCCT AAACTTAGTT CTGTGATAAG GGATGGTGCT 
GGTCACACTT CTTTGTCTCG GCCCAAGTCA CTGTTGGTGT CATCTCCATC TACAAAACTA 
CTAAGCCTAG AGGAGGCTCA AGCAAGGACA CAGGCCCAAA TCAATTCCCC TGTTGTGGCT 
GACAGCAAGT ACATTGAGGT GGGAGAGGGA CCAGCTGCTT TGCAGGGCAG ATTCCACACT 
GTAATTGACT TCCCTTCTGA AAGGAAAAGA CCGCCAACCA AAATGAAAAA ATCTCCTGTT 
GGAAGCTGGC GTTCTTTTTT TAGCATAGGC AAATCATCAT CTTCAGCCTC CATGTCTAAG 
CGGAAACTTC AGCGTAATCC AAGTGAGCCT TTGGAAATGA AATCCATGGC ATTTGCAGGT 
GGAAGAGATA GCAGCACCAT GCGCTCAGCT AAAAGCGAGG AGTCTCTCAG TTCCTTGCAT 
GCAGCAGATG GAGAGTCGAA GCTATTTCGT CCACGTCGTC CTCGTTCCAG CAGCGATGCT 
CTGTCTGCTT CATATAATGG GGACCTTCTG GATAGCTGCA ACCGTTGCAA CTCCTATGAT 
AATCTGCCCC GAGACCAAGA CAGTGATGGA GATGAGGGTT TGATTCATGT GCCTGCTATG 
CTGTCAGGTC GCTCTCCTGA GGATGAAGAA CTCAGCCCAC CAGACATTGG TATGGCCAGT 
TTGGACTTTG ACCCAATGTC TTTTCAGTGC AGCCCTCCCC AGCTAGAATC TGAAGGACCA 
GACATTTTTT TTCAGCTTGA CATTTGTGCT AGCAATGGCA AGGATAAGGT GCAAAGTGAT 
GCACAAAACC TAGGCGGTGT CTCCAGCTCC GAACCTCACT CTCCTTTCCA AGAGAAAGTC 
GTTAGTCCAT TTCTATCGCC TGACCGCAGT TCTAGTACAG ATAAAGTATC CAAAACTACA 
TCATTTGCAG AAAAAGTTGT GCAGGCCTTG TCACCTAAAG TGGGGCGCAA AGCAGTTCGA 
TCTCCTCCAT TAACCATCTC AGAGCCTGTC ACCATCTCTC TCCCTAGTCG GGTATCGGAA 
ATGATTGGAA GTATGCAAGG CAATACTGCT GCTTCCCAGT CTTTAATTTG GTACAGAGAA 
AACAGTTTAA ATTCTAGAGA AACTGAAATG AATTATGTTT GTAAATCGGT GGATGCCAAC 
CTTCCATCCT TATTGCCAGC AACAGATCCA TGGTCTACAG TTACTCCTGT AATTACTCAA 
TGGAACCCAG ATGGATTACA GAGAGAAGAC AAAGCAGGAC CATTTACTCA TGGCTTTCCC 
CAGCCAAATA GTGACTTGGA ACAAGTAGAA AACTTCAACC TGGAACTTAG CACAACAAAT 
GTTGCTGCAG CATATCAGAA AGAAGATACA GAATTGTCGA GAGCAAATCA GAACAAATCC 
CACTCTTCTG TTTCTCCCCC TCAAGGAGCT TCTGCTTCAG AATCTCCACA GGAGCTAGCC 
CCTGTCAGTA GTGTTTCTAT CATTCCACCT CCACCTCCTC CAAAGAATCC TGCACGCATG 
CTGGCATTAG CACTGGCTGA ATCTGCTCAA CAGGCATCTG CTAATAAAAG ACCTCTTTAT 
ATGCAGTTCA TGGATCTACC CCCCCATCCT CCTCCACCAT CAGTACTAGA GGACAAACCA 
CTTCTTGAGT TATCTCCAAA AATGTCTCCA GAGCACCTTG TATCTGGTCC TGCAACACCA 
GCGACCAGTC CTCCAGTCAT ATCCAATAAA ACATCTCCAG CTACTCCACC AAGCACAACT 
TCATCATTTT CTATCACAAC AATTCATCAC AGTCCTGTGA AAAACCATAG TCCTCTGGCA 
GGACAGATGC CTACAGCAGT CACTCCTGGA CTCAGTCCTT CATCAAACCA GGTTCCTGTA 
TCCTTTCTAT CTGATGAAAT GCTACCTTCT GAAACAAATA AAATGAATTC TGTCATACTG 
AGTGAAGCAT TTACAACAGG AATTCCTGTT ACTCCACCAT CTGAAAAACC CAAGGAAATT 
TCTAGACCAC CACATCTTCA CCAGCGTTCT GAATCCTTTC CTTCACATCC TGCATATAGC 
AATGCAAAGC CCACTCCACC AGTTAGAACA ATGGATAGTC GACTGGCAAC TGCCATGCAT 
TCCAACTTCA ATGATGCCAT CACTGCTAAT AACTACCACT CCTTCCTGAA CACCATGATG 
CTTCCTTCTT CTCTAGAGGA TGTGCTACCC AGACACAACT ATTCTCATTC TTCTCATCTC 
AAGACAGAAA ATATAGATGA ACAGGGAGTT AATTACAGAC AACCTTATTT ATCCAACACC 
AAGCAAGAGG ACCCAGCTGA TGTGGGAGAT TTATACAGAC AGCAATATAT TTCTACTGGA 
AAGTCTGAGA ATGCTGAGAT AGGAAACACT TACCGGCACC CATATCCTTC CAATACTGAG 
CCAGAAAGTC TAAAGGAGCC ACTAGATCCT TTTCTGCATC ACAAGTCAGC AGTTGCTCCA 
AAGACATATA GAGCAGAAAC ACTTCTACCA CATATGCCTC CTCAAGTTTA TGGTTCAAGA 
TGTGATACTC CATCCTCAAC ATTTTATGGC ACCTACATAA ATCAGGCCAA ACACCCCCGC 
ACCAGGAACA GACCTGATTA TATGTCCTCT ATGAGCCCAG GTCTCCGTAG CTACTCAGAA 
GACACATCTT CCTACCCTAC TATTCGAAGG GTACAATCTC TGCATGTGCC TATGCAACCT 
CCGCCACCTC CTATCCGCAC TGTTCCAATT TCAAGAACAG AGGTTCCCCC AGATGATGAT 
CCAGCATACT GCCCAAGACC AGTTTATCAA TACAAACAAT GCCCCCAATT TAACTCTCAG 
TCAGATTACC ATGTTACCCA GCTGCAGCCT TACTTTGAAA ATGGACGAGT TCATTATCGC 
TACAGTCCCT ATTCAGGGGG CCCAAGTTAT TTTTCAGCAG ATAGCAATTT CTATGATATG 
GACCCCTACA GCACCTTACG TCTGCGACAA CTTCCTCTCT ACCCAAGCCG GGACTTTGCT 
TCTTACACCA CAAGATTGCA GCCCAAAAAC ACATATCGGT CACAAGGATT GCCCCCATAT 
CCCAGAGGAA CTCTACGGGA ACACAATTTT ATCAGTAGAG ATGTTCCTCC TGCACTACCA 
CCTGAACATC CACGACCTCT TCACATTTCT TGGGACATGG AAGACATGGA CAGATACAGA 
CTGCAATCCC TGCGACGAGA GAACAGGGCA CGTCAGAGAG CTAAAGGACC AGTAATGTCT 
CAGTATGATA ATGTTACACC AACCTTAGTG GAGGATGTAG CAGGCTTAGA TGTCATACAT 
CTCCGAAGCC GTTCTGATCC TGGTAAGACT CCTGGTCTTT TAAGTGTGGC TGAGAACAAA 
GACGTCCGTT ACCCAGGAAG GGCAGAAGGT GATGAGCATA CAACATATCC ACCTCCTGTT 
TTTGGAAATG GCCATCAAGA TAAGCCATCT CTTCCTCAAA AGCAAAGTGG ATCAAGCCGG 
GTTCGAATGC AACATGAAAT ATCTACTGAG CAGCGGACTC AACCTTCTCA AGATACTTTG 
CATAGGCAAC CCACAGAGGG ACGAAATGGT CCTCCAATTC CTCCTGTGGA CTACAGCTCT 
AAAGGAATGC CAAATCCCCC AGACCCTGCA TCATATCATT CCTCTGCAAA CAAATATAAC 
TTGACACAAC AGGAACCCAT GCGACTTATT CACAAAGAGC TTCGGCTCCC TGAGGATATT 
GAGCATTCAC ATACCAGGCC AGCTGACAAC CAACACCGTG ACTGTTACAG GGAGGAGCCA 
GCTCAGTTTC CCCTTACTGC ACCTCCTCCT AAACCAGAGA GGAGCCATAG CCTTCGAGGT 
CATAATCCAG ATGTTTTAGA AAGAGACCCT AGTATCTTCT ACCCCTACCA AACGCTTCAA 
GCCAAGAGAC ACAGCACAAT TAATGCAGTG TCACAATATG ATAATCTAGC TGATTACCAT 
TCTATACCCC ATCATCGAAC CACTAACCAG TCTACTCCAA ATGCCTTTAA CCTCCCACAT 
GGACGGACCT ATGCTACTGC CCTGGGCCAG GGTGCCTTTC TAGCTGCTGA ACTCGGCTTG 
CAAAGGCCTG AGACAAAAAT CCATGTGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_018095929.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951418.2 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X6, mRNA.

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CloneID	OXa61964
Clone ID Related Accession (Same CDS sequence)	XM_018095929.2
Accession Version	XM_018095929.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018095929.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGAA TAATGAAGTC TTCACTGCAC TTAGAGGAGG ATGATTTTGT ACCCGAGTTG 
CCAAGGAGTA TCCATCCCCG GGAGAGGCCG GACTGGGAGG AGACTATTAG TGCCATGGCA 
CGAGGGGCAG ACATCCCAGT CCCAGGGGAA ATACCGACGA TGCCTACAGA AATCCCTCTT 
CGCTCCTGTG GCAGTACTGC CAGCATGAAG GTGAAAAATG TCAAGAAGTT ATCCTTCACG 
AAAGGGCATT TTCCTAAACT TGCAGAATGT GCACATTTCC ACTATGAAAA TGTAGACTTT 
GGCAGCATAC AGCTCACACT CACAGAAGAT CAAAATGAAG TGAGCAGAAA TGGCTGTGAA 
TCCAAGGAAC TTGTTTTCCT AGTACAGATT GCTTGTCAGG GCCGTAGCTG GATAGTTAAG 
CGGAGTTATG AAGATTTTCG GGTTCTGGAT AAACACCTTC ATCTGTGCAT ATATGATAGG 
CGCTTCTCTC AGCTCTCAGA GCTGCCCCGT TCCGACTCCG TAAAGGATAG TCCAGAGTTG 
GTCACACAGA TGCTGATGGC TTACCTATCG CGGCTGTCAG CCATCGCAGG TAACAAGATC 
AATTGTGGGC CAGCTCTGAC GTGGATGGAG ATCGATAATA AAGGCAATCA TTTGCTTGTG 
CACGAAGAGT CCTCCATAAA TGTTCCAGCC ATCGCTGCTG CCCATGTCAT AAAGCGATAT 
AATGCTCAGG CCCCAGATGA ACTCTCTTTT GAGGTGGGAG ACATAGTATC TGTAATTGAC 
ATGCCACCAA AAGAACTGAC CACGTGGTGG AGAGGAAAGC ATGGATTTCA AGTGGGTTTC 
TTTCCAAGTG AATGTGTGGA ATTAATAAAT GATAAAGTCC CCCAATCAAT GACAAACTCT 
GTGCCTAAAC CAGTCTCCCC TGCGCATGGA ATGAGGCCGG CCTCCTGGAG CTACTTTCCC 
CCCTATTTGG AGATGGATAG TTTAAGGCCT GGAACATCGT GCTGTTCTGA GGTATTAAGT 
CACTCGCAGC TAAGCTCAGT GTCCCGGAAG CACGGCAAGC TCATCACATT TCTAAGAACC 
TTCATGAAGT CCAGACCCAG CAAACAGAAA CTGAAGCAGA GAGGGATCCT GAGGGAGCGA 
GTGTTTGGTT GTGACCTTGG GGAACATCTG CTTAACTCTG GTCAGGATGT CCCCCAAGTC 
CTCAGGAGCT GCACAGAGTT TATAGAAAAA CATGGCATTG TTGATGGAAT CTACCGTCTG 
TCTGGGATTG CATCTAACAT CCAGAAACTA CGTCATGAAT TTGACTCTGA ACAGATTCCA 
GACCTGACTA AAGATGTGTA CATCCAGGAC ATTCACTGTG TTGGCTCTCT CTGTAAACTC 
TACTTCCGGG AACTCCCAAA CCCTCTGCTC ACATATCAGT TGTATGAAAA GTTCTCTGAT 
GCTGTGTCAG CGGCTACTGA TGAAGAGAGA CTGGTGAAAA TTCATGATGT TATTCAACAG 
CTTCCCCCAC CTCATTACAG AACACTGGAG TTCCTTATGC GCCACTTATC AAGACTTGCG 
ACATACTGCT CTATCACCAA TATGCACACT AAAAATTTGG CAATTGTCTG GGCCCCTAAC 
CTGCTCCGGT CAAAACAGAT TGAGTCTGCA TGTTTTAGCG GTACAGCAGC TTTCATGGAA 
GTGCGCATAC AGTCTGTGGT AGTTGAATTT ATTCTAAACC ATGTGGAGGT TCTCTTTAGT 
CCTAAACTTA GTTCTGTGAT AAGGGATGGT GCTGGTCACA CTTCTTTGTC TCGGCCCAAG 
TCACTGTTGG TGTCATCTCC ATCTACAAAA CTACTAAGCC TAGAGGAGGC TCAAGCAAGG 
ACACAGGCCC AAATCAATTC CCCTGTTGTG GCTGACAGCA AGTACATTGA GGTGGGAGAG 
GGACCAGCTG CTTTGCAGGG CAGATTCCAC ACTGTAATTG ACTTCCCTTC TGAAAGGAAA 
AGACCGCCAA CCAAAATGAA AAAATCTCCT GTTGGAAGCT GGCGTTCTTT TTTTAGCATA 
GGCAAATCAT CATCTTCAGC CTCCATGTCT AAGCGGAAAC TTCAGCGTAA TCCAAGTGAG 
CCTTTGGAAA TGAAATCCAT GGCATTTGCA GGTGGAAGAG ATAGCAGCAC CATGCGCTCA 
GCTAAAAGCG AGGAGTCTCT CAGTTCCTTG CATGCAGCAG ATGGAGAGTC GAAGCTATTT 
CGTCCACGTC GTCCTCGTTC CAGCAGCGAT GCTCTGTCTG CTTCATATAA TGGGGACCTT 
CTGGATAGCT GCAACCGTTG CAACTCCTAT GATAATCTGC CCCGAGACCA AGACAGTGAT 
GGAGATGAGG GTTTGATTCA TGTGCCTGCT ATGCTGTCAG GTCGCTCTCC TGAGGATGAA 
GAACTCAGCC CACCAGACAT TGGTATGGCC AGTTTGGACT TTGACCCAAT GTCTTTTCAG 
TGCAGCCCTC CCCAGCTAGA ATCTGAAGGA CCAGACATTT TTTTTCAGCT TGACATTTGT 
GCTAGCAATG GCAAGGATAA GGTGCAAAGT GATGCACAAA ACCTAGGCGG TGTCTCCAGC 
TCCGAACCTC ACTCTCCTTT CCAAGAGAAA GTCGTTAGTC CATTTCTATC GCCTGACCGC 
AGTTCTAGTA CAGATAAAGT ATCCAAAACT ACATCATTTG CAGAAAAAGT TGTGCAGGCC 
TTGTCACCTA AAGTGGGGCG CAAAGCAGTT CGATCTCCTC CATTAACCAT CTCAGAGCCT 
GTCACCATCT CTCTCCCTAG TCGGGTATCG GAAATGATTG GAAGTATGCA AGGCAATACT 
GCTGCTTCCC AGTCTTTAAT TTGGTACAGA GAAAACAGTT TAAATTCTAG AGAAACTGAA 
ATGAATTATG TTTGTAAATC GGTGGATGCC AACCTTCCAT CCTTATTGCC AGCAACAGAT 
CCATGGTCTA CAGTTACTCC TGTAATTACT CAATGGAACC CAGATGGATT ACAGAGAGAA 
GACAAAGCAG GACCATTTAC TCATGGCTTT CCCCAGCCAA ATAGTGACTT GGAACAAGTA 
GAAAACTTCA ACCTGGAACT TAGCACAACA AATGTTGCTG CAGCATATCA GAAAGAAGAT 
ACAGAATTGT CGAGAGCAAA TCAGAACAAA TCCCACTCTT CTGTTTCTCC CCCTCAAGGA 
GCTTCTGCTT CAGAATCTCC ACAGGAGCTA GCCCCTGTCA GTAGTGTTTC TATCATTCCA 
CCTCCACCTC CTCCAAAGAA TCCTGCACGC ATGCTGGCAT TAGCACTGGC TGAATCTGCT 
CAACAGGCAT CTGCTAATAA AAGACCTCTT TATATGCAGT TCATGGATCT ACCCCCCCAT 
CCTCCTCCAC CATCAGTACT AGAGGACAAA CCACTTCTTG AGTTATCTCC AAAAATGTCT 
CCAGAGCACC TTGTATCTGG TCCTGCAACA CCAGCGACCA GTCCTCCAGT CATATCCAAT 
AAAACATCTC CAGCTACTCC ACCAAGCACA ACTTCATCAT TTTCTATCAC AACAATTCAT 
CACAGTCCTG TGAAAAACCA TAGTCCTCTG GCAGGACAGA TGCCTACAGC AGTCACTCCT 
GGACTCAGTC CTTCATCAAA CCAGGTTCCT GTATCCTTTC TATCTGATGA AATGCTACCT 
TCTGAAACAA ATAAAATGAA TTCTGTCATA CTGAGTGAAG CATTTACAAC AGGAATTCCT 
GTTACTCCAC CATCTGAAAA ACCCAAGGAA ATTTCTAGAC CACCACATCT TCACCAGCGT 
TCTGAATCCT TTCCTTCACA TCCTGCATAT AGCAATGCAA AGCCCACTCC ACCAGTTAGA 
ACAATGGATA GTCGACTGGC AACTGCCATG CATTCCAACT TCAATGATGC CATCACTGCT 
AATAACTACC ACTCCTTCCT GAACACCATG ATGCTTCCTT CTTCTCTAGA GGATGTGCTA 
CCCAGACACA ACTATTCTCA TTCTTCTCAT CTCAAGACAG AAAATATAGA TGAACAGGGA 
GTTAATTACA GACAACCTTA TTTATCCAAC ACCAAGCAAG AGGACCCAGC TGATGTGGGA 
GATTTATACA GACAGCAATA TATTTCTACT GGAAAGTCTG AGAATGCTGA GATAGGAAAC 
ACTTACCGGC ACCCATATCC TTCCAATACT GAGCCAGAAA GTCTAAAGGA GCCACTAGAT 
CCTTTTCTGC ATCACAAGTC AGCAGTTGCT CCAAAGACAT ATAGAGCAGA AACACTTCTA 
CCACATATGC CTCCTCAAGT TTATGGTTCA AGATGTGATA CTCCATCCTC AACATTTTAT 
GGCACCTACA TAAATCAGGC CAAACACCCC CGCACCAGGA ACAGACCTGA TTATATGTCC 
TCTATGAGCC CAGGTCTCCG TAGCTACTCA GAAGACACAT CTTCCTACCC TACTATTCGA 
AGGGTACAAT CTCTGCATGT GCCTATGCAA CCTCCGCCAC CTCCTATCCG CACTGTTCCA 
ATTTCAAGAA CAGAGGTTCC CCCAGATGAT GATCCAGCAT ACTGCCCAAG ACCAGTTTAT 
CAATACAAAC AATGCCCCCA ATTTAACTCT CAGTCAGATT ACCATGTTAC CCAGCTGCAG 
CCTTACTTTG AAAATGGACG AGTTCATTAT CGCTACAGTC CCTATTCAGG GGGCCCAAGT 
TATTTTTCAG CAGATAGCAA TTTCTATGAT ATGGACCCCT ACAGCACCTT ACGTCTGCGA 
CAACTTCCTC TCTACCCAAG CCGGGACTTT GCTTCTTACA CCACAAGATT GCAGCCCAAA 
AACACATATC GGTCACAAGG ATTGCCCCCA TATCCCAGAG GAACTCTACG GGAACACAAT 
TTTATCAGTA GAGATGTTCC TCCTGCACTA CCACCTGAAC ATCCACGACC TCTTCACATT 
TCTTGGGACA TGGAAGACAT GGACAGATAC AGACTGCAAT CCCTGCGACG AGAGAACAGG 
GCACGTCAGA GAGCTAAAGG ACCAGTAATG TCTCAGTATG ATAATGTTAC ACCAACCTTA 
GTGGAGGATG TAGCAGGCTT AGATGTCATA CATCTCCGAA GCCGTTCTGA TCCTGGTAAG 
ACTCCTGGTC TTTTAAGTGT GGCTGAGAAC AAAGACGTCC GTTACCCAGG AAGGGCAGAA 
GGTGATGAGC ATACAACATA TCCACCTCCT GTTTTTGGAA ATGGCCATCA AGATAAGCCA 
TCTCTTCCTC AAAAGCAAAG TGGATCAAGC CGGGTTCGAA TGCAACATGA AATATCTACT 
GAGCAGCGGA CTCAACCTTC TCAAGATACT TTGCATAGGC AACCCACAGA GGGACGAAAT 
GGTCCTCCAA TTCCTCCTGT GGACTACAGC TCTAAAGGAA TGCCAAATCC CCCAGACCCT 
GCATCATATC ATTCCTCTGC AAACAAATAT AACTTGACAC AACAGGAACC CATGCGACTT 
ATTCACAAAG AGCTTCGGCT CCCTGAGGAT ATTGAGCATT CACATACCAG GCCAGCTGAC 
AACCAACACC GTGACTGTTA CAGGGAGGAG CCAGCTCAGT TTCCCCTTAC TGCACCTCCT 
CCTAAACCAG AGAGGAGCCA TAGCCTTCGA GGTCATAATC CAGATGTTTT AGAAAGAGAC 
CCTAGTATCT TCTACCCCTA CCAAACGCTT CAAGCCAAGA GACACAGCAC AATTAATGCA 
GTGTCACAAT ATGATAATCT AGCTGATTAC CATTCTATAC CCCATCATCG AACCACTAAC 
CAGTCTACTC CAAATGCCTT TAACCTCCCA CATGGACGGA CCTATGCTAC TGCCCTGGGC 
CAGGGTGCCT TTCTAGCTGC TGAACTCGGC TTGCAAAGGC CTGAGACAAA AATCCATGTG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951418.2
CDS	343..6228
Translation	MYRIMKSSLHLEEDDFVPELPRSIHPRERPDWEETISAMARGADIPVPGEIPTMPTEIPLRSCGSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGSIQLTLTEDQNEVSRNGCESKELVFLVQIACQGRSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLSELPRSDSVKDSPELVTQMLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINVPAIAAAHVIKRYNAQAPDELSFEVGDIVSVIDMPPKELTTWWRGKHGFQVGFFPSECVELINDKVPQSMTNSVPKPVSPAHGMRPASWSYFPPYLEMDSLRPGTSCCSEVLSHSQLSSVSRKHGKLITFLRTFMKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSDAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_018095929.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095929.2

ORF Insert Sequence:

ATGTACAGAA TAATGAAGTC TTCACTGCAC TTAGAGGAGG ATGATTTTGT ACCCGAGTTG 
CCAAGGAGTA TCCATCCCCG GGAGAGGCCG GACTGGGAGG AGACTATTAG TGCCATGGCA 
CGAGGGGCAG ACATCCCAGT CCCAGGGGAA ATACCGACGA TGCCTACAGA AATCCCTCTT 
CGCTCCTGTG GCAGTACTGC CAGCATGAAG GTGAAAAATG TCAAGAAGTT ATCCTTCACG 
AAAGGGCATT TTCCTAAACT TGCAGAATGT GCACATTTCC ACTATGAAAA TGTAGACTTT 
GGCAGCATAC AGCTCACACT CACAGAAGAT CAAAATGAAG TGAGCAGAAA TGGCTGTGAA 
TCCAAGGAAC TTGTTTTCCT AGTACAGATT GCTTGTCAGG GCCGTAGCTG GATAGTTAAG 
CGGAGTTATG AAGATTTTCG GGTTCTGGAT AAACACCTTC ATCTGTGCAT ATATGATAGG 
CGCTTCTCTC AGCTCTCAGA GCTGCCCCGT TCCGACTCCG TAAAGGATAG TCCAGAGTTG 
GTCACACAGA TGCTGATGGC TTACCTATCG CGGCTGTCAG CCATCGCAGG TAACAAGATC 
AATTGTGGGC CAGCTCTGAC GTGGATGGAG ATCGATAATA AAGGCAATCA TTTGCTTGTG 
CACGAAGAGT CCTCCATAAA TGTTCCAGCC ATCGCTGCTG CCCATGTCAT AAAGCGATAT 
AATGCTCAGG CCCCAGATGA ACTCTCTTTT GAGGTGGGAG ACATAGTATC TGTAATTGAC 
ATGCCACCAA AAGAACTGAC CACGTGGTGG AGAGGAAAGC ATGGATTTCA AGTGGGTTTC 
TTTCCAAGTG AATGTGTGGA ATTAATAAAT GATAAAGTCC CCCAATCAAT GACAAACTCT 
GTGCCTAAAC CAGTCTCCCC TGCGCATGGA ATGAGGCCGG CCTCCTGGAG CTACTTTCCC 
CCCTATTTGG AGATGGATAG TTTAAGGCCT GGAACATCGT GCTGTTCTGA GGTATTAAGT 
CACTCGCAGC TAAGCTCAGT GTCCCGGAAG CACGGCAAGC TCATCACATT TCTAAGAACC 
TTCATGAAGT CCAGACCCAG CAAACAGAAA CTGAAGCAGA GAGGGATCCT GAGGGAGCGA 
GTGTTTGGTT GTGACCTTGG GGAACATCTG CTTAACTCTG GTCAGGATGT CCCCCAAGTC 
CTCAGGAGCT GCACAGAGTT TATAGAAAAA CATGGCATTG TTGATGGAAT CTACCGTCTG 
TCTGGGATTG CATCTAACAT CCAGAAACTA CGTCATGAAT TTGACTCTGA ACAGATTCCA 
GACCTGACTA AAGATGTGTA CATCCAGGAC ATTCACTGTG TTGGCTCTCT CTGTAAACTC 
TACTTCCGGG AACTCCCAAA CCCTCTGCTC ACATATCAGT TGTATGAAAA GTTCTCTGAT 
GCTGTGTCAG CGGCTACTGA TGAAGAGAGA CTGGTGAAAA TTCATGATGT TATTCAACAG 
CTTCCCCCAC CTCATTACAG AACACTGGAG TTCCTTATGC GCCACTTATC AAGACTTGCG 
ACATACTGCT CTATCACCAA TATGCACACT AAAAATTTGG CAATTGTCTG GGCCCCTAAC 
CTGCTCCGGT CAAAACAGAT TGAGTCTGCA TGTTTTAGCG GTACAGCAGC TTTCATGGAA 
GTGCGCATAC AGTCTGTGGT AGTTGAATTT ATTCTAAACC ATGTGGAGGT TCTCTTTAGT 
CCTAAACTTA GTTCTGTGAT AAGGGATGGT GCTGGTCACA CTTCTTTGTC TCGGCCCAAG 
TCACTGTTGG TGTCATCTCC ATCTACAAAA CTACTAAGCC TAGAGGAGGC TCAAGCAAGG 
ACACAGGCCC AAATCAATTC CCCTGTTGTG GCTGACAGCA AGTACATTGA GGTGGGAGAG 
GGACCAGCTG CTTTGCAGGG CAGATTCCAC ACTGTAATTG ACTTCCCTTC TGAAAGGAAA 
AGACCGCCAA CCAAAATGAA AAAATCTCCT GTTGGAAGCT GGCGTTCTTT TTTTAGCATA 
GGCAAATCAT CATCTTCAGC CTCCATGTCT AAGCGGAAAC TTCAGCGTAA TCCAAGTGAG 
CCTTTGGAAA TGAAATCCAT GGCATTTGCA GGTGGAAGAG ATAGCAGCAC CATGCGCTCA 
GCTAAAAGCG AGGAGTCTCT CAGTTCCTTG CATGCAGCAG ATGGAGAGTC GAAGCTATTT 
CGTCCACGTC GTCCTCGTTC CAGCAGCGAT GCTCTGTCTG CTTCATATAA TGGGGACCTT 
CTGGATAGCT GCAACCGTTG CAACTCCTAT GATAATCTGC CCCGAGACCA AGACAGTGAT 
GGAGATGAGG GTTTGATTCA TGTGCCTGCT ATGCTGTCAG GTCGCTCTCC TGAGGATGAA 
GAACTCAGCC CACCAGACAT TGGTATGGCC AGTTTGGACT TTGACCCAAT GTCTTTTCAG 
TGCAGCCCTC CCCAGCTAGA ATCTGAAGGA CCAGACATTT TTTTTCAGCT TGACATTTGT 
GCTAGCAATG GCAAGGATAA GGTGCAAAGT GATGCACAAA ACCTAGGCGG TGTCTCCAGC 
TCCGAACCTC ACTCTCCTTT CCAAGAGAAA GTCGTTAGTC CATTTCTATC GCCTGACCGC 
AGTTCTAGTA CAGATAAAGT ATCCAAAACT ACATCATTTG CAGAAAAAGT TGTGCAGGCC 
TTGTCACCTA AAGTGGGGCG CAAAGCAGTT CGATCTCCTC CATTAACCAT CTCAGAGCCT 
GTCACCATCT CTCTCCCTAG TCGGGTATCG GAAATGATTG GAAGTATGCA AGGCAATACT 
GCTGCTTCCC AGTCTTTAAT TTGGTACAGA GAAAACAGTT TAAATTCTAG AGAAACTGAA 
ATGAATTATG TTTGTAAATC GGTGGATGCC AACCTTCCAT CCTTATTGCC AGCAACAGAT 
CCATGGTCTA CAGTTACTCC TGTAATTACT CAATGGAACC CAGATGGATT ACAGAGAGAA 
GACAAAGCAG GACCATTTAC TCATGGCTTT CCCCAGCCAA ATAGTGACTT GGAACAAGTA 
GAAAACTTCA ACCTGGAACT TAGCACAACA AATGTTGCTG CAGCATATCA GAAAGAAGAT 
ACAGAATTGT CGAGAGCAAA TCAGAACAAA TCCCACTCTT CTGTTTCTCC CCCTCAAGGA 
GCTTCTGCTT CAGAATCTCC ACAGGAGCTA GCCCCTGTCA GTAGTGTTTC TATCATTCCA 
CCTCCACCTC CTCCAAAGAA TCCTGCACGC ATGCTGGCAT TAGCACTGGC TGAATCTGCT 
CAACAGGCAT CTGCTAATAA AAGACCTCTT TATATGCAGT TCATGGATCT ACCCCCCCAT 
CCTCCTCCAC CATCAGTACT AGAGGACAAA CCACTTCTTG AGTTATCTCC AAAAATGTCT 
CCAGAGCACC TTGTATCTGG TCCTGCAACA CCAGCGACCA GTCCTCCAGT CATATCCAAT 
AAAACATCTC CAGCTACTCC ACCAAGCACA ACTTCATCAT TTTCTATCAC AACAATTCAT 
CACAGTCCTG TGAAAAACCA TAGTCCTCTG GCAGGACAGA TGCCTACAGC AGTCACTCCT 
GGACTCAGTC CTTCATCAAA CCAGGTTCCT GTATCCTTTC TATCTGATGA AATGCTACCT 
TCTGAAACAA ATAAAATGAA TTCTGTCATA CTGAGTGAAG CATTTACAAC AGGAATTCCT 
GTTACTCCAC CATCTGAAAA ACCCAAGGAA ATTTCTAGAC CACCACATCT TCACCAGCGT 
TCTGAATCCT TTCCTTCACA TCCTGCATAT AGCAATGCAA AGCCCACTCC ACCAGTTAGA 
ACAATGGATA GTCGACTGGC AACTGCCATG CATTCCAACT TCAATGATGC CATCACTGCT 
AATAACTACC ACTCCTTCCT GAACACCATG ATGCTTCCTT CTTCTCTAGA GGATGTGCTA 
CCCAGACACA ACTATTCTCA TTCTTCTCAT CTCAAGACAG AAAATATAGA TGAACAGGGA 
GTTAATTACA GACAACCTTA TTTATCCAAC ACCAAGCAAG AGGACCCAGC TGATGTGGGA 
GATTTATACA GACAGCAATA TATTTCTACT GGAAAGTCTG AGAATGCTGA GATAGGAAAC 
ACTTACCGGC ACCCATATCC TTCCAATACT GAGCCAGAAA GTCTAAAGGA GCCACTAGAT 
CCTTTTCTGC ATCACAAGTC AGCAGTTGCT CCAAAGACAT ATAGAGCAGA AACACTTCTA 
CCACATATGC CTCCTCAAGT TTATGGTTCA AGATGTGATA CTCCATCCTC AACATTTTAT 
GGCACCTACA TAAATCAGGC CAAACACCCC CGCACCAGGA ACAGACCTGA TTATATGTCC 
TCTATGAGCC CAGGTCTCCG TAGCTACTCA GAAGACACAT CTTCCTACCC TACTATTCGA 
AGGGTACAAT CTCTGCATGT GCCTATGCAA CCTCCGCCAC CTCCTATCCG CACTGTTCCA 
ATTTCAAGAA CAGAGGTTCC CCCAGATGAT GATCCAGCAT ACTGCCCAAG ACCAGTTTAT 
CAATACAAAC AATGCCCCCA ATTTAACTCT CAGTCAGATT ACCATGTTAC CCAGCTGCAG 
CCTTACTTTG AAAATGGACG AGTTCATTAT CGCTACAGTC CCTATTCAGG GGGCCCAAGT 
TATTTTTCAG CAGATAGCAA TTTCTATGAT ATGGACCCCT ACAGCACCTT ACGTCTGCGA 
CAACTTCCTC TCTACCCAAG CCGGGACTTT GCTTCTTACA CCACAAGATT GCAGCCCAAA 
AACACATATC GGTCACAAGG ATTGCCCCCA TATCCCAGAG GAACTCTACG GGAACACAAT 
TTTATCAGTA GAGATGTTCC TCCTGCACTA CCACCTGAAC ATCCACGACC TCTTCACATT 
TCTTGGGACA TGGAAGACAT GGACAGATAC AGACTGCAAT CCCTGCGACG AGAGAACAGG 
GCACGTCAGA GAGCTAAAGG ACCAGTAATG TCTCAGTATG ATAATGTTAC ACCAACCTTA 
GTGGAGGATG TAGCAGGCTT AGATGTCATA CATCTCCGAA GCCGTTCTGA TCCTGGTAAG 
ACTCCTGGTC TTTTAAGTGT GGCTGAGAAC AAAGACGTCC GTTACCCAGG AAGGGCAGAA 
GGTGATGAGC ATACAACATA TCCACCTCCT GTTTTTGGAA ATGGCCATCA AGATAAGCCA 
TCTCTTCCTC AAAAGCAAAG TGGATCAAGC CGGGTTCGAA TGCAACATGA AATATCTACT 
GAGCAGCGGA CTCAACCTTC TCAAGATACT TTGCATAGGC AACCCACAGA GGGACGAAAT 
GGTCCTCCAA TTCCTCCTGT GGACTACAGC TCTAAAGGAA TGCCAAATCC CCCAGACCCT 
GCATCATATC ATTCCTCTGC AAACAAATAT AACTTGACAC AACAGGAACC CATGCGACTT 
ATTCACAAAG AGCTTCGGCT CCCTGAGGAT ATTGAGCATT CACATACCAG GCCAGCTGAC 
AACCAACACC GTGACTGTTA CAGGGAGGAG CCAGCTCAGT TTCCCCTTAC TGCACCTCCT 
CCTAAACCAG AGAGGAGCCA TAGCCTTCGA GGTCATAATC CAGATGTTTT AGAAAGAGAC 
CCTAGTATCT TCTACCCCTA CCAAACGCTT CAAGCCAAGA GACACAGCAC AATTAATGCA 
GTGTCACAAT ATGATAATCT AGCTGATTAC CATTCTATAC CCCATCATCG AACCACTAAC 
CAGTCTACTC CAAATGCCTT TAACCTCCCA CATGGACGGA CCTATGCTAC TGCCCTGGGC 
CAGGGTGCCT TTCTAGCTGC TGAACTCGGC TTGCAAAGGC CTGAGACAAA AATCCATGTG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_031905577.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761437.1 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X7, mRNA.

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CloneID	OXa61965
Clone ID Related Accession (Same CDS sequence)	XM_031905577.1 , XM_004916168.4
Accession Version	XM_031905577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGTGTCC 
CGGAAGCACG GCAAGCTCAT CACATTTCTA AGAACCTTCA TGAAGTCCAG ACCCAGCAAA 
CAGAAACTGA AGCAGAGAGG GATCCTGAGG GAGCGAGTGT TTGGTTGTGA CCTTGGGGAA 
CATCTGCTTA ACTCTGGTCA GGATGTCCCC CAAGTCCTCA GGAGCTGCAC AGAGTTTATA 
GAAAAACATG GCATTGTTGA TGGAATCTAC CGTCTGTCTG GGATTGCATC TAACATCCAG 
AAACTACGTC ATGAATTTGA CTCTGAACAG ATTCCAGACC TGACTAAAGA TGTGTACATC 
CAGGACATTC ACTGTGTTGG CTCTCTCTGT AAACTCTACT TCCGGGAACT CCCAAACCCT 
CTGCTCACAT ATCAGTTGTA TGAAAAGTTC TCTGATGCTG TGTCAGCGGC TACTGATGAA 
GAGAGACTGG TGAAAATTCA TGATGTTATT CAACAGCTTC CCCCACCTCA TTACAGAACA 
CTGGAGTTCC TTATGCGCCA CTTATCAAGA CTTGCGACAT ACTGCTCTAT CACCAATATG 
CACACTAAAA ATTTGGCAAT TGTCTGGGCC CCTAACCTGC TCCGGTCAAA ACAGATTGAG 
TCTGCATGTT TTAGCGGTAC AGCAGCTTTC ATGGAAGTGC GCATACAGTC TGTGGTAGTT 
GAATTTATTC TAAACCATGT GGAGGTTCTC TTTAGTCCTA AACTTAGTTC TGTGATAAGG 
GATGGTGCTG GTCACACTTC TTTGTCTCGG CCCAAGTCAC TGTTGGTGTC ATCTCCATCT 
ACAAAACTAC TAAGCCTAGA GGAGGCTCAA GCAAGGACAC AGGCCCAAAT CAATTCCCCT 
GTTGTGGCTG ACAGCAAGTA CATTGAGGTG GGAGAGGGAC CAGCTGCTTT GCAGGGCAGA 
TTCCACACTG TAATTGACTT CCCTTCTGAA AGGAAAAGAC CGCCAACCAA AATGAAAAAA 
TCTCCTGTTG GAAGCTGGCG TTCTTTTTTT AGCATAGGCA AATCATCATC TTCAGCCTCC 
ATGTCTAAGC GGAAACTTCA GCGTAATCCA AGTGAGCCTT TGGAAATGAA ATCCATGGCA 
TTTGCAGGTG GAAGAGATAG CAGCACCATG CGCTCAGCTA AAAGCGAGGA GTCTCTCAGT 
TCCTTGCATG CAGCAGATGG AGAGTCGAAG CTATTTCGTC CACGTCGTCC TCGTTCCAGC 
AGCGATGCTC TGTCTGCTTC ATATAATGGG GACCTTCTGG ATAGCTGCAA CCGTTGCAAC 
TCCTATGATA ATCTGCCCCG AGACCAAGAC AGTGATGGAG ATGAGGGTTT GATTCATGTG 
CCTGCTATGC TGTCAGGTCG CTCTCCTGAG GATGAAGAAC TCAGCCCACC AGACATTGGT 
ATGGCCAGTT TGGACTTTGA CCCAATGTCT TTTCAGTGCA GCCCTCCCCA GCTAGAATCT 
GAAGGACCAG ACATTTTTTT TCAGCTTGAC ATTTGTGCTA GCAATGGCAA GGATAAGGTG 
CAAAGTGATG CACAAAACCT AGGCGGTGTC TCCAGCTCCG AACCTCACTC TCCTTTCCAA 
GAGAAAGTCG TTAGTCCATT TCTATCGCCT GACCGCAGTT CTAGTACAGA TAAAGTATCC 
AAAACTACAT CATTTGCAGA AAAAGTTGTG CAGGCCTTGT CACCTAAAGT GGGGCGCAAA 
GCAGTTCGAT CTCCTCCATT AACCATCTCA GAGCCTGTCA CCATCTCTCT CCCTAGTCGG 
GTATCGGAAA TGATTGGAAG TATGCAAGGC AATACTGCTG CTTCCCAGTC TTTAATTTGG 
TACAGAGAAA ACAGTTTAAA TTCTAGAGAA ACTGAAATGA ATTATGTTTG TAAATCGGTG 
GATGCCAACC TTCCATCCTT ATTGCCAGCA ACAGATCCAT GGTCTACAGT TACTCCTGTA 
ATTACTCAAT GGAACCCAGA TGGATTACAG AGAGAAGACA AAGCAGGACC ATTTACTCAT 
GGCTTTCCCC AGCCAAATAG TGACTTGGAA CAAGTAGAAA ACTTCAACCT GGAACTTAGC 
ACAACAAATG TTGCTGCAGC ATATCAGAAA GAAGATACAG AATTGTCGAG AGCAAATCAG 
AACAAATCCC ACTCTTCTGT TTCTCCCCCT CAAGGAGCTT CTGCTTCAGA ATCTCCACAG 
GAGCTAGCCC CTGTCAGTAG TGTTTCTATC ATTCCACCTC CACCTCCTCC AAAGAATCCT 
GCACGCATGC TGGCATTAGC ACTGGCTGAA TCTGCTCAAC AGGCATCTGC TAATAAAAGA 
CCTCTTTATA TGCAGTTCAT GGATCTACCC CCCCATCCTC CTCCACCATC AGTACTAGAG 
GACAAACCAC TTCTTGAGTT ATCTCCAAAA ATGTCTCCAG AGCACCTTGT ATCTGGTCCT 
GCAACACCAG CGACCAGTCC TCCAGTCATA TCCAATAAAA CATCTCCAGC TACTCCACCA 
AGCACAACTT CATCATTTTC TATCACAACA ATTCATCACA GTCCTGTGAA AAACCATAGT 
CCTCTGGCAG GACAGATGCC TACAGCAGTC ACTCCTGGAC TCAGTCCTTC ATCAAACCAG 
GTTCCTGTAT CCTTTCTATC TGATGAAATG CTACCTTCTG AAACAAATAA AATGAATTCT 
GTCATACTGA GTGAAGCATT TACAACAGGA ATTCCTGTTA CTCCACCATC TGAAAAACCC 
AAGGAAATTT CTAGACCACC ACATCTTCAC CAGCGTTCTG AATCCTTTCC TTCACATCCT 
GCATATAGCA ATGCAAAGCC CACTCCACCA GTTAGAACAA TGGATAGTCG ACTGGCAACT 
GCCATGCATT CCAACTTCAA TGATGCCATC ACTGCTAATA ACTACCACTC CTTCCTGAAC 
ACCATGATGC TTCCTTCTTC TCTAGAGGAT GTGCTACCCA GACACAACTA TTCTCATTCT 
TCTCATCTCA AGACAGAAAA TATAGATGAA CAGGGAGTTA ATTACAGACA ACCTTATTTA 
TCCAACACCA AGCAAGAGGA CCCAGCTGAT GTGGGAGATT TATACAGACA GCAATATATT 
TCTACTGGAA AGTCTGAGAA TGCTGAGATA GGAAACACTT ACCGGCACCC ATATCCTTCC 
AATACTGAGC CAGAAAGTCT AAAGGAGCCA CTAGATCCTT TTCTGCATCA CAAGTCAGCA 
GTTGCTCCAA AGACATATAG AGCAGAAACA CTTCTACCAC ATATGCCTCC TCAAGTTTAT 
GGTTCAAGAT GTGATACTCC ATCCTCAACA TTTTATGGCA CCTACATAAA TCAGGCCAAA 
CACCCCCGCA CCAGGAACAG ACCTGATTAT ATGTCCTCTA TGAGCCCAGG TCTCCGTAGC 
TACTCAGAAG ACACATCTTC CTACCCTACT ATTCGAAGGG TACAATCTCT GCATGTGCCT 
ATGCAACCTC CGCCACCTCC TATCCGCACT GTTCCAATTT CAAGAACAGA GGTTCCCCCA 
GATGATGATC CAGCATACTG CCCAAGACCA GTTTATCAAT ACAAACAATG CCCCCAATTT 
AACTCTCAGT CAGATTACCA TGTTACCCAG CTGCAGCCTT ACTTTGAAAA TGGACGAGTT 
CATTATCGCT ACAGTCCCTA TTCAGGGGGC CCAAGTTATT TTTCAGCAGA TAGCAATTTC 
TATGATATGG ACCCCTACAG CACCTTACGT CTGCGACAAC TTCCTCTCTA CCCAAGCCGG 
GACTTTGCTT CTTACACCAC AAGATTGCAG CCCAAAAACA CATATCGGTC ACAAGGATTG 
CCCCCATATC CCAGAGGAAC TCTACGGGAA CACAATTTTA TCAGTAGAGA TGTTCCTCCT 
GCACTACCAC CTGAACATCC ACGACCTCTT CACATTTCTT GGGACATGGA AGACATGGAC 
AGATACAGAC TGCAATCCCT GCGACGAGAG AACAGGGCAC GTCAGAGAGC TAAAGGACCA 
GTAATGTCTC AGTATGATAA TGTTACACCA ACCTTAGTGG AGGATGTAGC AGGCTTAGAT 
GTCATACATC TCCGAAGCCG TTCTGATCCT GGTAAGACTC CTGGTCTTTT AAGTGTGGCT 
GAGAACAAAG ACGTCCGTTA CCCAGGAAGG GCAGAAGGTG ATGAGCATAC AACATATCCA 
CCTCCTGTTT TTGGAAATGG CCATCAAGAT AAGCCATCTC TTCCTCAAAA GCAAAGTGGA 
TCAAGCCGGG TTCGAATGCA ACATGAAATA TCTACTGAGC AGCGGACTCA ACCTTCTCAA 
GATACTTTGC ATAGGCAACC CACAGAGGGA CGAAATGGTC CTCCAATTCC TCCTGTGGAC 
TACAGCTCTA AAGGAATGCC AAATCCCCCA GACCCTGCAT CATATCATTC CTCTGCAAAC 
AAATATAACT TGACACAACA GGAACCCATG CGACTTATTC ACAAAGAGCT TCGGCTCCCT 
GAGGATATTG AGCATTCACA TACCAGGCCA GCTGACAACC AACACCGTGA CTGTTACAGG 
GAGGAGCCAG CTCAGTTTCC CCTTACTGCA CCTCCTCCTA AACCAGAGAG GAGCCATAGC 
CTTCGAGGTC ATAATCCAGA TGTTTTAGAA AGAGACCCTA GTATCTTCTA CCCCTACCAA 
ACGCTTCAAG CCAAGAGACA CAGCACAATT AATGCAGTGT CACAATATGA TAATCTAGCT 
GATTACCATT CTATACCCCA TCATCGAACC ACTAACCAGT CTACTCCAAA TGCCTTTAAC 
CTCCCACATG GACGGACCTA TGCTACTGCC CTGGGCCAGG GTGCCTTTCT AGCTGCTGAA 
CTCGGCTTGC AAAGGCCTGA GACAAAAATC CATGTGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761437.1
CDS	1380..7241
Translation	MEGGTDAAAFAAFPSIHAVPSTSGRMATGGKLDELERIMKSSLHLEEDDFVPELPRSIHPRERPDWEETISAMARGADIPVPGEIPTMPTEIPLRSCGSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGSIQLTLTEDQNEVSRNGCESKELVFLVQIACQGRSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLSELPRSDSVKDSPELVTQMLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINVPAIAAAHVIKRYNAQAPDELSFEVGDIVSVIDMPPKELTTWWRGKHGFQVGFFPSECVELINDKVPQSMTNSVPKPVSRKHGKLITFLRTFMKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSDAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_031905577.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905577.1

ORF Insert Sequence:

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGTGTCC 
CGGAAGCACG GCAAGCTCAT CACATTTCTA AGAACCTTCA TGAAGTCCAG ACCCAGCAAA 
CAGAAACTGA AGCAGAGAGG GATCCTGAGG GAGCGAGTGT TTGGTTGTGA CCTTGGGGAA 
CATCTGCTTA ACTCTGGTCA GGATGTCCCC CAAGTCCTCA GGAGCTGCAC AGAGTTTATA 
GAAAAACATG GCATTGTTGA TGGAATCTAC CGTCTGTCTG GGATTGCATC TAACATCCAG 
AAACTACGTC ATGAATTTGA CTCTGAACAG ATTCCAGACC TGACTAAAGA TGTGTACATC 
CAGGACATTC ACTGTGTTGG CTCTCTCTGT AAACTCTACT TCCGGGAACT CCCAAACCCT 
CTGCTCACAT ATCAGTTGTA TGAAAAGTTC TCTGATGCTG TGTCAGCGGC TACTGATGAA 
GAGAGACTGG TGAAAATTCA TGATGTTATT CAACAGCTTC CCCCACCTCA TTACAGAACA 
CTGGAGTTCC TTATGCGCCA CTTATCAAGA CTTGCGACAT ACTGCTCTAT CACCAATATG 
CACACTAAAA ATTTGGCAAT TGTCTGGGCC CCTAACCTGC TCCGGTCAAA ACAGATTGAG 
TCTGCATGTT TTAGCGGTAC AGCAGCTTTC ATGGAAGTGC GCATACAGTC TGTGGTAGTT 
GAATTTATTC TAAACCATGT GGAGGTTCTC TTTAGTCCTA AACTTAGTTC TGTGATAAGG 
GATGGTGCTG GTCACACTTC TTTGTCTCGG CCCAAGTCAC TGTTGGTGTC ATCTCCATCT 
ACAAAACTAC TAAGCCTAGA GGAGGCTCAA GCAAGGACAC AGGCCCAAAT CAATTCCCCT 
GTTGTGGCTG ACAGCAAGTA CATTGAGGTG GGAGAGGGAC CAGCTGCTTT GCAGGGCAGA 
TTCCACACTG TAATTGACTT CCCTTCTGAA AGGAAAAGAC CGCCAACCAA AATGAAAAAA 
TCTCCTGTTG GAAGCTGGCG TTCTTTTTTT AGCATAGGCA AATCATCATC TTCAGCCTCC 
ATGTCTAAGC GGAAACTTCA GCGTAATCCA AGTGAGCCTT TGGAAATGAA ATCCATGGCA 
TTTGCAGGTG GAAGAGATAG CAGCACCATG CGCTCAGCTA AAAGCGAGGA GTCTCTCAGT 
TCCTTGCATG CAGCAGATGG AGAGTCGAAG CTATTTCGTC CACGTCGTCC TCGTTCCAGC 
AGCGATGCTC TGTCTGCTTC ATATAATGGG GACCTTCTGG ATAGCTGCAA CCGTTGCAAC 
TCCTATGATA ATCTGCCCCG AGACCAAGAC AGTGATGGAG ATGAGGGTTT GATTCATGTG 
CCTGCTATGC TGTCAGGTCG CTCTCCTGAG GATGAAGAAC TCAGCCCACC AGACATTGGT 
ATGGCCAGTT TGGACTTTGA CCCAATGTCT TTTCAGTGCA GCCCTCCCCA GCTAGAATCT 
GAAGGACCAG ACATTTTTTT TCAGCTTGAC ATTTGTGCTA GCAATGGCAA GGATAAGGTG 
CAAAGTGATG CACAAAACCT AGGCGGTGTC TCCAGCTCCG AACCTCACTC TCCTTTCCAA 
GAGAAAGTCG TTAGTCCATT TCTATCGCCT GACCGCAGTT CTAGTACAGA TAAAGTATCC 
AAAACTACAT CATTTGCAGA AAAAGTTGTG CAGGCCTTGT CACCTAAAGT GGGGCGCAAA 
GCAGTTCGAT CTCCTCCATT AACCATCTCA GAGCCTGTCA CCATCTCTCT CCCTAGTCGG 
GTATCGGAAA TGATTGGAAG TATGCAAGGC AATACTGCTG CTTCCCAGTC TTTAATTTGG 
TACAGAGAAA ACAGTTTAAA TTCTAGAGAA ACTGAAATGA ATTATGTTTG TAAATCGGTG 
GATGCCAACC TTCCATCCTT ATTGCCAGCA ACAGATCCAT GGTCTACAGT TACTCCTGTA 
ATTACTCAAT GGAACCCAGA TGGATTACAG AGAGAAGACA AAGCAGGACC ATTTACTCAT 
GGCTTTCCCC AGCCAAATAG TGACTTGGAA CAAGTAGAAA ACTTCAACCT GGAACTTAGC 
ACAACAAATG TTGCTGCAGC ATATCAGAAA GAAGATACAG AATTGTCGAG AGCAAATCAG 
AACAAATCCC ACTCTTCTGT TTCTCCCCCT CAAGGAGCTT CTGCTTCAGA ATCTCCACAG 
GAGCTAGCCC CTGTCAGTAG TGTTTCTATC ATTCCACCTC CACCTCCTCC AAAGAATCCT 
GCACGCATGC TGGCATTAGC ACTGGCTGAA TCTGCTCAAC AGGCATCTGC TAATAAAAGA 
CCTCTTTATA TGCAGTTCAT GGATCTACCC CCCCATCCTC CTCCACCATC AGTACTAGAG 
GACAAACCAC TTCTTGAGTT ATCTCCAAAA ATGTCTCCAG AGCACCTTGT ATCTGGTCCT 
GCAACACCAG CGACCAGTCC TCCAGTCATA TCCAATAAAA CATCTCCAGC TACTCCACCA 
AGCACAACTT CATCATTTTC TATCACAACA ATTCATCACA GTCCTGTGAA AAACCATAGT 
CCTCTGGCAG GACAGATGCC TACAGCAGTC ACTCCTGGAC TCAGTCCTTC ATCAAACCAG 
GTTCCTGTAT CCTTTCTATC TGATGAAATG CTACCTTCTG AAACAAATAA AATGAATTCT 
GTCATACTGA GTGAAGCATT TACAACAGGA ATTCCTGTTA CTCCACCATC TGAAAAACCC 
AAGGAAATTT CTAGACCACC ACATCTTCAC CAGCGTTCTG AATCCTTTCC TTCACATCCT 
GCATATAGCA ATGCAAAGCC CACTCCACCA GTTAGAACAA TGGATAGTCG ACTGGCAACT 
GCCATGCATT CCAACTTCAA TGATGCCATC ACTGCTAATA ACTACCACTC CTTCCTGAAC 
ACCATGATGC TTCCTTCTTC TCTAGAGGAT GTGCTACCCA GACACAACTA TTCTCATTCT 
TCTCATCTCA AGACAGAAAA TATAGATGAA CAGGGAGTTA ATTACAGACA ACCTTATTTA 
TCCAACACCA AGCAAGAGGA CCCAGCTGAT GTGGGAGATT TATACAGACA GCAATATATT 
TCTACTGGAA AGTCTGAGAA TGCTGAGATA GGAAACACTT ACCGGCACCC ATATCCTTCC 
AATACTGAGC CAGAAAGTCT AAAGGAGCCA CTAGATCCTT TTCTGCATCA CAAGTCAGCA 
GTTGCTCCAA AGACATATAG AGCAGAAACA CTTCTACCAC ATATGCCTCC TCAAGTTTAT 
GGTTCAAGAT GTGATACTCC ATCCTCAACA TTTTATGGCA CCTACATAAA TCAGGCCAAA 
CACCCCCGCA CCAGGAACAG ACCTGATTAT ATGTCCTCTA TGAGCCCAGG TCTCCGTAGC 
TACTCAGAAG ACACATCTTC CTACCCTACT ATTCGAAGGG TACAATCTCT GCATGTGCCT 
ATGCAACCTC CGCCACCTCC TATCCGCACT GTTCCAATTT CAAGAACAGA GGTTCCCCCA 
GATGATGATC CAGCATACTG CCCAAGACCA GTTTATCAAT ACAAACAATG CCCCCAATTT 
AACTCTCAGT CAGATTACCA TGTTACCCAG CTGCAGCCTT ACTTTGAAAA TGGACGAGTT 
CATTATCGCT ACAGTCCCTA TTCAGGGGGC CCAAGTTATT TTTCAGCAGA TAGCAATTTC 
TATGATATGG ACCCCTACAG CACCTTACGT CTGCGACAAC TTCCTCTCTA CCCAAGCCGG 
GACTTTGCTT CTTACACCAC AAGATTGCAG CCCAAAAACA CATATCGGTC ACAAGGATTG 
CCCCCATATC CCAGAGGAAC TCTACGGGAA CACAATTTTA TCAGTAGAGA TGTTCCTCCT 
GCACTACCAC CTGAACATCC ACGACCTCTT CACATTTCTT GGGACATGGA AGACATGGAC 
AGATACAGAC TGCAATCCCT GCGACGAGAG AACAGGGCAC GTCAGAGAGC TAAAGGACCA 
GTAATGTCTC AGTATGATAA TGTTACACCA ACCTTAGTGG AGGATGTAGC AGGCTTAGAT 
GTCATACATC TCCGAAGCCG TTCTGATCCT GGTAAGACTC CTGGTCTTTT AAGTGTGGCT 
GAGAACAAAG ACGTCCGTTA CCCAGGAAGG GCAGAAGGTG ATGAGCATAC AACATATCCA 
CCTCCTGTTT TTGGAAATGG CCATCAAGAT AAGCCATCTC TTCCTCAAAA GCAAAGTGGA 
TCAAGCCGGG TTCGAATGCA ACATGAAATA TCTACTGAGC AGCGGACTCA ACCTTCTCAA 
GATACTTTGC ATAGGCAACC CACAGAGGGA CGAAATGGTC CTCCAATTCC TCCTGTGGAC 
TACAGCTCTA AAGGAATGCC AAATCCCCCA GACCCTGCAT CATATCATTC CTCTGCAAAC 
AAATATAACT TGACACAACA GGAACCCATG CGACTTATTC ACAAAGAGCT TCGGCTCCCT 
GAGGATATTG AGCATTCACA TACCAGGCCA GCTGACAACC AACACCGTGA CTGTTACAGG 
GAGGAGCCAG CTCAGTTTCC CCTTACTGCA CCTCCTCCTA AACCAGAGAG GAGCCATAGC 
CTTCGAGGTC ATAATCCAGA TGTTTTAGAA AGAGACCCTA GTATCTTCTA CCCCTACCAA 
ACGCTTCAAG CCAAGAGACA CAGCACAATT AATGCAGTGT CACAATATGA TAATCTAGCT 
GATTACCATT CTATACCCCA TCATCGAACC ACTAACCAGT CTACTCCAAA TGCCTTTAAC 
CTCCCACATG GACGGACCTA TGCTACTGCC CTGGGCCAGG GTGCCTTTCT AGCTGCTGAA 
CTCGGCTTGC AAAGGCCTGA GACAAAAATC CATGTGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_004916168.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916225.2 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OXa61965
Clone ID Related Accession (Same CDS sequence)	XM_031905577.1 , XM_004916168.4
Accession Version	XM_004916168.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916168.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGTGTCC 
CGGAAGCACG GCAAGCTCAT CACATTTCTA AGAACCTTCA TGAAGTCCAG ACCCAGCAAA 
CAGAAACTGA AGCAGAGAGG GATCCTGAGG GAGCGAGTGT TTGGTTGTGA CCTTGGGGAA 
CATCTGCTTA ACTCTGGTCA GGATGTCCCC CAAGTCCTCA GGAGCTGCAC AGAGTTTATA 
GAAAAACATG GCATTGTTGA TGGAATCTAC CGTCTGTCTG GGATTGCATC TAACATCCAG 
AAACTACGTC ATGAATTTGA CTCTGAACAG ATTCCAGACC TGACTAAAGA TGTGTACATC 
CAGGACATTC ACTGTGTTGG CTCTCTCTGT AAACTCTACT TCCGGGAACT CCCAAACCCT 
CTGCTCACAT ATCAGTTGTA TGAAAAGTTC TCTGATGCTG TGTCAGCGGC TACTGATGAA 
GAGAGACTGG TGAAAATTCA TGATGTTATT CAACAGCTTC CCCCACCTCA TTACAGAACA 
CTGGAGTTCC TTATGCGCCA CTTATCAAGA CTTGCGACAT ACTGCTCTAT CACCAATATG 
CACACTAAAA ATTTGGCAAT TGTCTGGGCC CCTAACCTGC TCCGGTCAAA ACAGATTGAG 
TCTGCATGTT TTAGCGGTAC AGCAGCTTTC ATGGAAGTGC GCATACAGTC TGTGGTAGTT 
GAATTTATTC TAAACCATGT GGAGGTTCTC TTTAGTCCTA AACTTAGTTC TGTGATAAGG 
GATGGTGCTG GTCACACTTC TTTGTCTCGG CCCAAGTCAC TGTTGGTGTC ATCTCCATCT 
ACAAAACTAC TAAGCCTAGA GGAGGCTCAA GCAAGGACAC AGGCCCAAAT CAATTCCCCT 
GTTGTGGCTG ACAGCAAGTA CATTGAGGTG GGAGAGGGAC CAGCTGCTTT GCAGGGCAGA 
TTCCACACTG TAATTGACTT CCCTTCTGAA AGGAAAAGAC CGCCAACCAA AATGAAAAAA 
TCTCCTGTTG GAAGCTGGCG TTCTTTTTTT AGCATAGGCA AATCATCATC TTCAGCCTCC 
ATGTCTAAGC GGAAACTTCA GCGTAATCCA AGTGAGCCTT TGGAAATGAA ATCCATGGCA 
TTTGCAGGTG GAAGAGATAG CAGCACCATG CGCTCAGCTA AAAGCGAGGA GTCTCTCAGT 
TCCTTGCATG CAGCAGATGG AGAGTCGAAG CTATTTCGTC CACGTCGTCC TCGTTCCAGC 
AGCGATGCTC TGTCTGCTTC ATATAATGGG GACCTTCTGG ATAGCTGCAA CCGTTGCAAC 
TCCTATGATA ATCTGCCCCG AGACCAAGAC AGTGATGGAG ATGAGGGTTT GATTCATGTG 
CCTGCTATGC TGTCAGGTCG CTCTCCTGAG GATGAAGAAC TCAGCCCACC AGACATTGGT 
ATGGCCAGTT TGGACTTTGA CCCAATGTCT TTTCAGTGCA GCCCTCCCCA GCTAGAATCT 
GAAGGACCAG ACATTTTTTT TCAGCTTGAC ATTTGTGCTA GCAATGGCAA GGATAAGGTG 
CAAAGTGATG CACAAAACCT AGGCGGTGTC TCCAGCTCCG AACCTCACTC TCCTTTCCAA 
GAGAAAGTCG TTAGTCCATT TCTATCGCCT GACCGCAGTT CTAGTACAGA TAAAGTATCC 
AAAACTACAT CATTTGCAGA AAAAGTTGTG CAGGCCTTGT CACCTAAAGT GGGGCGCAAA 
GCAGTTCGAT CTCCTCCATT AACCATCTCA GAGCCTGTCA CCATCTCTCT CCCTAGTCGG 
GTATCGGAAA TGATTGGAAG TATGCAAGGC AATACTGCTG CTTCCCAGTC TTTAATTTGG 
TACAGAGAAA ACAGTTTAAA TTCTAGAGAA ACTGAAATGA ATTATGTTTG TAAATCGGTG 
GATGCCAACC TTCCATCCTT ATTGCCAGCA ACAGATCCAT GGTCTACAGT TACTCCTGTA 
ATTACTCAAT GGAACCCAGA TGGATTACAG AGAGAAGACA AAGCAGGACC ATTTACTCAT 
GGCTTTCCCC AGCCAAATAG TGACTTGGAA CAAGTAGAAA ACTTCAACCT GGAACTTAGC 
ACAACAAATG TTGCTGCAGC ATATCAGAAA GAAGATACAG AATTGTCGAG AGCAAATCAG 
AACAAATCCC ACTCTTCTGT TTCTCCCCCT CAAGGAGCTT CTGCTTCAGA ATCTCCACAG 
GAGCTAGCCC CTGTCAGTAG TGTTTCTATC ATTCCACCTC CACCTCCTCC AAAGAATCCT 
GCACGCATGC TGGCATTAGC ACTGGCTGAA TCTGCTCAAC AGGCATCTGC TAATAAAAGA 
CCTCTTTATA TGCAGTTCAT GGATCTACCC CCCCATCCTC CTCCACCATC AGTACTAGAG 
GACAAACCAC TTCTTGAGTT ATCTCCAAAA ATGTCTCCAG AGCACCTTGT ATCTGGTCCT 
GCAACACCAG CGACCAGTCC TCCAGTCATA TCCAATAAAA CATCTCCAGC TACTCCACCA 
AGCACAACTT CATCATTTTC TATCACAACA ATTCATCACA GTCCTGTGAA AAACCATAGT 
CCTCTGGCAG GACAGATGCC TACAGCAGTC ACTCCTGGAC TCAGTCCTTC ATCAAACCAG 
GTTCCTGTAT CCTTTCTATC TGATGAAATG CTACCTTCTG AAACAAATAA AATGAATTCT 
GTCATACTGA GTGAAGCATT TACAACAGGA ATTCCTGTTA CTCCACCATC TGAAAAACCC 
AAGGAAATTT CTAGACCACC ACATCTTCAC CAGCGTTCTG AATCCTTTCC TTCACATCCT 
GCATATAGCA ATGCAAAGCC CACTCCACCA GTTAGAACAA TGGATAGTCG ACTGGCAACT 
GCCATGCATT CCAACTTCAA TGATGCCATC ACTGCTAATA ACTACCACTC CTTCCTGAAC 
ACCATGATGC TTCCTTCTTC TCTAGAGGAT GTGCTACCCA GACACAACTA TTCTCATTCT 
TCTCATCTCA AGACAGAAAA TATAGATGAA CAGGGAGTTA ATTACAGACA ACCTTATTTA 
TCCAACACCA AGCAAGAGGA CCCAGCTGAT GTGGGAGATT TATACAGACA GCAATATATT 
TCTACTGGAA AGTCTGAGAA TGCTGAGATA GGAAACACTT ACCGGCACCC ATATCCTTCC 
AATACTGAGC CAGAAAGTCT AAAGGAGCCA CTAGATCCTT TTCTGCATCA CAAGTCAGCA 
GTTGCTCCAA AGACATATAG AGCAGAAACA CTTCTACCAC ATATGCCTCC TCAAGTTTAT 
GGTTCAAGAT GTGATACTCC ATCCTCAACA TTTTATGGCA CCTACATAAA TCAGGCCAAA 
CACCCCCGCA CCAGGAACAG ACCTGATTAT ATGTCCTCTA TGAGCCCAGG TCTCCGTAGC 
TACTCAGAAG ACACATCTTC CTACCCTACT ATTCGAAGGG TACAATCTCT GCATGTGCCT 
ATGCAACCTC CGCCACCTCC TATCCGCACT GTTCCAATTT CAAGAACAGA GGTTCCCCCA 
GATGATGATC CAGCATACTG CCCAAGACCA GTTTATCAAT ACAAACAATG CCCCCAATTT 
AACTCTCAGT CAGATTACCA TGTTACCCAG CTGCAGCCTT ACTTTGAAAA TGGACGAGTT 
CATTATCGCT ACAGTCCCTA TTCAGGGGGC CCAAGTTATT TTTCAGCAGA TAGCAATTTC 
TATGATATGG ACCCCTACAG CACCTTACGT CTGCGACAAC TTCCTCTCTA CCCAAGCCGG 
GACTTTGCTT CTTACACCAC AAGATTGCAG CCCAAAAACA CATATCGGTC ACAAGGATTG 
CCCCCATATC CCAGAGGAAC TCTACGGGAA CACAATTTTA TCAGTAGAGA TGTTCCTCCT 
GCACTACCAC CTGAACATCC ACGACCTCTT CACATTTCTT GGGACATGGA AGACATGGAC 
AGATACAGAC TGCAATCCCT GCGACGAGAG AACAGGGCAC GTCAGAGAGC TAAAGGACCA 
GTAATGTCTC AGTATGATAA TGTTACACCA ACCTTAGTGG AGGATGTAGC AGGCTTAGAT 
GTCATACATC TCCGAAGCCG TTCTGATCCT GGTAAGACTC CTGGTCTTTT AAGTGTGGCT 
GAGAACAAAG ACGTCCGTTA CCCAGGAAGG GCAGAAGGTG ATGAGCATAC AACATATCCA 
CCTCCTGTTT TTGGAAATGG CCATCAAGAT AAGCCATCTC TTCCTCAAAA GCAAAGTGGA 
TCAAGCCGGG TTCGAATGCA ACATGAAATA TCTACTGAGC AGCGGACTCA ACCTTCTCAA 
GATACTTTGC ATAGGCAACC CACAGAGGGA CGAAATGGTC CTCCAATTCC TCCTGTGGAC 
TACAGCTCTA AAGGAATGCC AAATCCCCCA GACCCTGCAT CATATCATTC CTCTGCAAAC 
AAATATAACT TGACACAACA GGAACCCATG CGACTTATTC ACAAAGAGCT TCGGCTCCCT 
GAGGATATTG AGCATTCACA TACCAGGCCA GCTGACAACC AACACCGTGA CTGTTACAGG 
GAGGAGCCAG CTCAGTTTCC CCTTACTGCA CCTCCTCCTA AACCAGAGAG GAGCCATAGC 
CTTCGAGGTC ATAATCCAGA TGTTTTAGAA AGAGACCCTA GTATCTTCTA CCCCTACCAA 
ACGCTTCAAG CCAAGAGACA CAGCACAATT AATGCAGTGT CACAATATGA TAATCTAGCT 
GATTACCATT CTATACCCCA TCATCGAACC ACTAACCAGT CTACTCCAAA TGCCTTTAAC 
CTCCCACATG GACGGACCTA TGCTACTGCC CTGGGCCAGG GTGCCTTTCT AGCTGCTGAA 
CTCGGCTTGC AAAGGCCTGA GACAAAAATC CATGTGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916225.2
CDS	1225..7086
Translation	MEGGTDAAAFAAFPSIHAVPSTSGRMATGGKLDELERIMKSSLHLEEDDFVPELPRSIHPRERPDWEETISAMARGADIPVPGEIPTMPTEIPLRSCGSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGSIQLTLTEDQNEVSRNGCESKELVFLVQIACQGRSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLSELPRSDSVKDSPELVTQMLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINVPAIAAAHVIKRYNAQAPDELSFEVGDIVSVIDMPPKELTTWWRGKHGFQVGFFPSECVELINDKVPQSMTNSVPKPVSRKHGKLITFLRTFMKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSDAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_004916168.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916168.4

ORF Insert Sequence:

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGTGTCC 
CGGAAGCACG GCAAGCTCAT CACATTTCTA AGAACCTTCA TGAAGTCCAG ACCCAGCAAA 
CAGAAACTGA AGCAGAGAGG GATCCTGAGG GAGCGAGTGT TTGGTTGTGA CCTTGGGGAA 
CATCTGCTTA ACTCTGGTCA GGATGTCCCC CAAGTCCTCA GGAGCTGCAC AGAGTTTATA 
GAAAAACATG GCATTGTTGA TGGAATCTAC CGTCTGTCTG GGATTGCATC TAACATCCAG 
AAACTACGTC ATGAATTTGA CTCTGAACAG ATTCCAGACC TGACTAAAGA TGTGTACATC 
CAGGACATTC ACTGTGTTGG CTCTCTCTGT AAACTCTACT TCCGGGAACT CCCAAACCCT 
CTGCTCACAT ATCAGTTGTA TGAAAAGTTC TCTGATGCTG TGTCAGCGGC TACTGATGAA 
GAGAGACTGG TGAAAATTCA TGATGTTATT CAACAGCTTC CCCCACCTCA TTACAGAACA 
CTGGAGTTCC TTATGCGCCA CTTATCAAGA CTTGCGACAT ACTGCTCTAT CACCAATATG 
CACACTAAAA ATTTGGCAAT TGTCTGGGCC CCTAACCTGC TCCGGTCAAA ACAGATTGAG 
TCTGCATGTT TTAGCGGTAC AGCAGCTTTC ATGGAAGTGC GCATACAGTC TGTGGTAGTT 
GAATTTATTC TAAACCATGT GGAGGTTCTC TTTAGTCCTA AACTTAGTTC TGTGATAAGG 
GATGGTGCTG GTCACACTTC TTTGTCTCGG CCCAAGTCAC TGTTGGTGTC ATCTCCATCT 
ACAAAACTAC TAAGCCTAGA GGAGGCTCAA GCAAGGACAC AGGCCCAAAT CAATTCCCCT 
GTTGTGGCTG ACAGCAAGTA CATTGAGGTG GGAGAGGGAC CAGCTGCTTT GCAGGGCAGA 
TTCCACACTG TAATTGACTT CCCTTCTGAA AGGAAAAGAC CGCCAACCAA AATGAAAAAA 
TCTCCTGTTG GAAGCTGGCG TTCTTTTTTT AGCATAGGCA AATCATCATC TTCAGCCTCC 
ATGTCTAAGC GGAAACTTCA GCGTAATCCA AGTGAGCCTT TGGAAATGAA ATCCATGGCA 
TTTGCAGGTG GAAGAGATAG CAGCACCATG CGCTCAGCTA AAAGCGAGGA GTCTCTCAGT 
TCCTTGCATG CAGCAGATGG AGAGTCGAAG CTATTTCGTC CACGTCGTCC TCGTTCCAGC 
AGCGATGCTC TGTCTGCTTC ATATAATGGG GACCTTCTGG ATAGCTGCAA CCGTTGCAAC 
TCCTATGATA ATCTGCCCCG AGACCAAGAC AGTGATGGAG ATGAGGGTTT GATTCATGTG 
CCTGCTATGC TGTCAGGTCG CTCTCCTGAG GATGAAGAAC TCAGCCCACC AGACATTGGT 
ATGGCCAGTT TGGACTTTGA CCCAATGTCT TTTCAGTGCA GCCCTCCCCA GCTAGAATCT 
GAAGGACCAG ACATTTTTTT TCAGCTTGAC ATTTGTGCTA GCAATGGCAA GGATAAGGTG 
CAAAGTGATG CACAAAACCT AGGCGGTGTC TCCAGCTCCG AACCTCACTC TCCTTTCCAA 
GAGAAAGTCG TTAGTCCATT TCTATCGCCT GACCGCAGTT CTAGTACAGA TAAAGTATCC 
AAAACTACAT CATTTGCAGA AAAAGTTGTG CAGGCCTTGT CACCTAAAGT GGGGCGCAAA 
GCAGTTCGAT CTCCTCCATT AACCATCTCA GAGCCTGTCA CCATCTCTCT CCCTAGTCGG 
GTATCGGAAA TGATTGGAAG TATGCAAGGC AATACTGCTG CTTCCCAGTC TTTAATTTGG 
TACAGAGAAA ACAGTTTAAA TTCTAGAGAA ACTGAAATGA ATTATGTTTG TAAATCGGTG 
GATGCCAACC TTCCATCCTT ATTGCCAGCA ACAGATCCAT GGTCTACAGT TACTCCTGTA 
ATTACTCAAT GGAACCCAGA TGGATTACAG AGAGAAGACA AAGCAGGACC ATTTACTCAT 
GGCTTTCCCC AGCCAAATAG TGACTTGGAA CAAGTAGAAA ACTTCAACCT GGAACTTAGC 
ACAACAAATG TTGCTGCAGC ATATCAGAAA GAAGATACAG AATTGTCGAG AGCAAATCAG 
AACAAATCCC ACTCTTCTGT TTCTCCCCCT CAAGGAGCTT CTGCTTCAGA ATCTCCACAG 
GAGCTAGCCC CTGTCAGTAG TGTTTCTATC ATTCCACCTC CACCTCCTCC AAAGAATCCT 
GCACGCATGC TGGCATTAGC ACTGGCTGAA TCTGCTCAAC AGGCATCTGC TAATAAAAGA 
CCTCTTTATA TGCAGTTCAT GGATCTACCC CCCCATCCTC CTCCACCATC AGTACTAGAG 
GACAAACCAC TTCTTGAGTT ATCTCCAAAA ATGTCTCCAG AGCACCTTGT ATCTGGTCCT 
GCAACACCAG CGACCAGTCC TCCAGTCATA TCCAATAAAA CATCTCCAGC TACTCCACCA 
AGCACAACTT CATCATTTTC TATCACAACA ATTCATCACA GTCCTGTGAA AAACCATAGT 
CCTCTGGCAG GACAGATGCC TACAGCAGTC ACTCCTGGAC TCAGTCCTTC ATCAAACCAG 
GTTCCTGTAT CCTTTCTATC TGATGAAATG CTACCTTCTG AAACAAATAA AATGAATTCT 
GTCATACTGA GTGAAGCATT TACAACAGGA ATTCCTGTTA CTCCACCATC TGAAAAACCC 
AAGGAAATTT CTAGACCACC ACATCTTCAC CAGCGTTCTG AATCCTTTCC TTCACATCCT 
GCATATAGCA ATGCAAAGCC CACTCCACCA GTTAGAACAA TGGATAGTCG ACTGGCAACT 
GCCATGCATT CCAACTTCAA TGATGCCATC ACTGCTAATA ACTACCACTC CTTCCTGAAC 
ACCATGATGC TTCCTTCTTC TCTAGAGGAT GTGCTACCCA GACACAACTA TTCTCATTCT 
TCTCATCTCA AGACAGAAAA TATAGATGAA CAGGGAGTTA ATTACAGACA ACCTTATTTA 
TCCAACACCA AGCAAGAGGA CCCAGCTGAT GTGGGAGATT TATACAGACA GCAATATATT 
TCTACTGGAA AGTCTGAGAA TGCTGAGATA GGAAACACTT ACCGGCACCC ATATCCTTCC 
AATACTGAGC CAGAAAGTCT AAAGGAGCCA CTAGATCCTT TTCTGCATCA CAAGTCAGCA 
GTTGCTCCAA AGACATATAG AGCAGAAACA CTTCTACCAC ATATGCCTCC TCAAGTTTAT 
GGTTCAAGAT GTGATACTCC ATCCTCAACA TTTTATGGCA CCTACATAAA TCAGGCCAAA 
CACCCCCGCA CCAGGAACAG ACCTGATTAT ATGTCCTCTA TGAGCCCAGG TCTCCGTAGC 
TACTCAGAAG ACACATCTTC CTACCCTACT ATTCGAAGGG TACAATCTCT GCATGTGCCT 
ATGCAACCTC CGCCACCTCC TATCCGCACT GTTCCAATTT CAAGAACAGA GGTTCCCCCA 
GATGATGATC CAGCATACTG CCCAAGACCA GTTTATCAAT ACAAACAATG CCCCCAATTT 
AACTCTCAGT CAGATTACCA TGTTACCCAG CTGCAGCCTT ACTTTGAAAA TGGACGAGTT 
CATTATCGCT ACAGTCCCTA TTCAGGGGGC CCAAGTTATT TTTCAGCAGA TAGCAATTTC 
TATGATATGG ACCCCTACAG CACCTTACGT CTGCGACAAC TTCCTCTCTA CCCAAGCCGG 
GACTTTGCTT CTTACACCAC AAGATTGCAG CCCAAAAACA CATATCGGTC ACAAGGATTG 
CCCCCATATC CCAGAGGAAC TCTACGGGAA CACAATTTTA TCAGTAGAGA TGTTCCTCCT 
GCACTACCAC CTGAACATCC ACGACCTCTT CACATTTCTT GGGACATGGA AGACATGGAC 
AGATACAGAC TGCAATCCCT GCGACGAGAG AACAGGGCAC GTCAGAGAGC TAAAGGACCA 
GTAATGTCTC AGTATGATAA TGTTACACCA ACCTTAGTGG AGGATGTAGC AGGCTTAGAT 
GTCATACATC TCCGAAGCCG TTCTGATCCT GGTAAGACTC CTGGTCTTTT AAGTGTGGCT 
GAGAACAAAG ACGTCCGTTA CCCAGGAAGG GCAGAAGGTG ATGAGCATAC AACATATCCA 
CCTCCTGTTT TTGGAAATGG CCATCAAGAT AAGCCATCTC TTCCTCAAAA GCAAAGTGGA 
TCAAGCCGGG TTCGAATGCA ACATGAAATA TCTACTGAGC AGCGGACTCA ACCTTCTCAA 
GATACTTTGC ATAGGCAACC CACAGAGGGA CGAAATGGTC CTCCAATTCC TCCTGTGGAC 
TACAGCTCTA AAGGAATGCC AAATCCCCCA GACCCTGCAT CATATCATTC CTCTGCAAAC 
AAATATAACT TGACACAACA GGAACCCATG CGACTTATTC ACAAAGAGCT TCGGCTCCCT 
GAGGATATTG AGCATTCACA TACCAGGCCA GCTGACAACC AACACCGTGA CTGTTACAGG 
GAGGAGCCAG CTCAGTTTCC CCTTACTGCA CCTCCTCCTA AACCAGAGAG GAGCCATAGC 
CTTCGAGGTC ATAATCCAGA TGTTTTAGAA AGAGACCCTA GTATCTTCTA CCCCTACCAA 
ACGCTTCAAG CCAAGAGACA CAGCACAATT AATGCAGTGT CACAATATGA TAATCTAGCT 
GATTACCATT CTATACCCCA TCATCGAACC ACTAACCAGT CTACTCCAAA TGCCTTTAAC 
CTCCCACATG GACGGACCTA TGCTACTGCC CTGGGCCAGG GTGCCTTTCT AGCTGCTGAA 
CTCGGCTTGC AAAGGCCTGA GACAAAAATC CATGTGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_012967128.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822582.2 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X9, mRNA.

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CloneID	OXa61966
Clone ID Related Accession (Same CDS sequence)	XM_012967128.2
Accession Version	XM_012967128.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012967128.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGAA TAATGAAGTC TTCACTGCAC TTAGAGGAGG ATGATTTTGT ACCCGAGTTG 
CCAAGGAGTA TCCATCCCCG GGAGAGGCCG GACTGGGAGG AGACTATTAG TGCCATGGCA 
CGAGGGGCAG ACATCCCAGT CCCAGGGGAA ATACCGACGA TGCCTACAGA AATCCCTCTT 
CGCTCCTGTG GCAGTACTGC CAGCATGAAG GTGAAAAATG TCAAGAAGTT ATCCTTCACG 
AAAGGGCATT TTCCTAAACT TGCAGAATGT GCACATTTCC ACTATGAAAA TGTAGACTTT 
GGCAGCATAC AGCTCACACT CACAGAAGAT CAAAATGAAG TGAGCAGAAA TGGCTGTGAA 
TCCAAGGAAC TTGTTTTCCT AGTACAGATT GCTTGTCAGG GCCGTAGCTG GATAGTTAAG 
CGGAGTTATG AAGATTTTCG GGTTCTGGAT AAACACCTTC ATCTGTGCAT ATATGATAGG 
CGCTTCTCTC AGCTCTCAGA GCTGCCCCGT TCCGACTCCG TAAAGGATAG TCCAGAGTTG 
GTCACACAGA TGCTGATGGC TTACCTATCG CGGCTGTCAG CCATCGCAGG TAACAAGATC 
AATTGTGGGC CAGCTCTGAC GTGGATGGAG ATCGATAATA AAGGCAATCA TTTGCTTGTG 
CACGAAGAGT CCTCCATAAA TGTTCCAGCC ATCGCTGCTG CCCATGTCAT AAAGCGATAT 
AATGCTCAGG CCCCAGATGA ACTCTCTTTT GAGGTGGGAG ACATAGTATC TGTAATTGAC 
ATGCCACCAA AAGAACTGAC CACGTGGTGG AGAGGAAAGC ATGGATTTCA AGTGGGTTTC 
TTTCCAAGTG AATGTGTGGA ATTAATAAAT GATAAAGTCC CCCAATCAAT GACAAACTCT 
GTGCCTAAAC CAGTGTCCCG GAAGCACGGC AAGCTCATCA CATTTCTAAG AACCTTCATG 
AAGTCCAGAC CCAGCAAACA GAAACTGAAG CAGAGAGGGA TCCTGAGGGA GCGAGTGTTT 
GGTTGTGACC TTGGGGAACA TCTGCTTAAC TCTGGTCAGG ATGTCCCCCA AGTCCTCAGG 
AGCTGCACAG AGTTTATAGA AAAACATGGC ATTGTTGATG GAATCTACCG TCTGTCTGGG 
ATTGCATCTA ACATCCAGAA ACTACGTCAT GAATTTGACT CTGAACAGAT TCCAGACCTG 
ACTAAAGATG TGTACATCCA GGACATTCAC TGTGTTGGCT CTCTCTGTAA ACTCTACTTC 
CGGGAACTCC CAAACCCTCT GCTCACATAT CAGTTGTATG AAAAGTTCTC TGATGCTGTG 
TCAGCGGCTA CTGATGAAGA GAGACTGGTG AAAATTCATG ATGTTATTCA ACAGCTTCCC 
CCACCTCATT ACAGAACACT GGAGTTCCTT ATGCGCCACT TATCAAGACT TGCGACATAC 
TGCTCTATCA CCAATATGCA CACTAAAAAT TTGGCAATTG TCTGGGCCCC TAACCTGCTC 
CGGTCAAAAC AGATTGAGTC TGCATGTTTT AGCGGTACAG CAGCTTTCAT GGAAGTGCGC 
ATACAGTCTG TGGTAGTTGA ATTTATTCTA AACCATGTGG AGGTTCTCTT TAGTCCTAAA 
CTTAGTTCTG TGATAAGGGA TGGTGCTGGT CACACTTCTT TGTCTCGGCC CAAGTCACTG 
TTGGTGTCAT CTCCATCTAC AAAACTACTA AGCCTAGAGG AGGCTCAAGC AAGGACACAG 
GCCCAAATCA ATTCCCCTGT TGTGGCTGAC AGCAAGTACA TTGAGGTGGG AGAGGGACCA 
GCTGCTTTGC AGGGCAGATT CCACACTGTA ATTGACTTCC CTTCTGAAAG GAAAAGACCG 
CCAACCAAAA TGAAAAAATC TCCTGTTGGA AGCTGGCGTT CTTTTTTTAG CATAGGCAAA 
TCATCATCTT CAGCCTCCAT GTCTAAGCGG AAACTTCAGC GTAATCCAAG TGAGCCTTTG 
GAAATGAAAT CCATGGCATT TGCAGGTGGA AGAGATAGCA GCACCATGCG CTCAGCTAAA 
AGCGAGGAGT CTCTCAGTTC CTTGCATGCA GCAGATGGAG AGTCGAAGCT ATTTCGTCCA 
CGTCGTCCTC GTTCCAGCAG CGATGCTCTG TCTGCTTCAT ATAATGGGGA CCTTCTGGAT 
AGCTGCAACC GTTGCAACTC CTATGATAAT CTGCCCCGAG ACCAAGACAG TGATGGAGAT 
GAGGGTTTGA TTCATGTGCC TGCTATGCTG TCAGGTCGCT CTCCTGAGGA TGAAGAACTC 
AGCCCACCAG ACATTGGTAT GGCCAGTTTG GACTTTGACC CAATGTCTTT TCAGTGCAGC 
CCTCCCCAGC TAGAATCTGA AGGACCAGAC ATTTTTTTTC AGCTTGACAT TTGTGCTAGC 
AATGGCAAGG ATAAGGTGCA AAGTGATGCA CAAAACCTAG GCGGTGTCTC CAGCTCCGAA 
CCTCACTCTC CTTTCCAAGA GAAAGTCGTT AGTCCATTTC TATCGCCTGA CCGCAGTTCT 
AGTACAGATA AAGTATCCAA AACTACATCA TTTGCAGAAA AAGTTGTGCA GGCCTTGTCA 
CCTAAAGTGG GGCGCAAAGC AGTTCGATCT CCTCCATTAA CCATCTCAGA GCCTGTCACC 
ATCTCTCTCC CTAGTCGGGT ATCGGAAATG ATTGGAAGTA TGCAAGGCAA TACTGCTGCT 
TCCCAGTCTT TAATTTGGTA CAGAGAAAAC AGTTTAAATT CTAGAGAAAC TGAAATGAAT 
TATGTTTGTA AATCGGTGGA TGCCAACCTT CCATCCTTAT TGCCAGCAAC AGATCCATGG 
TCTACAGTTA CTCCTGTAAT TACTCAATGG AACCCAGATG GATTACAGAG AGAAGACAAA 
GCAGGACCAT TTACTCATGG CTTTCCCCAG CCAAATAGTG ACTTGGAACA AGTAGAAAAC 
TTCAACCTGG AACTTAGCAC AACAAATGTT GCTGCAGCAT ATCAGAAAGA AGATACAGAA 
TTGTCGAGAG CAAATCAGAA CAAATCCCAC TCTTCTGTTT CTCCCCCTCA AGGAGCTTCT 
GCTTCAGAAT CTCCACAGGA GCTAGCCCCT GTCAGTAGTG TTTCTATCAT TCCACCTCCA 
CCTCCTCCAA AGAATCCTGC ACGCATGCTG GCATTAGCAC TGGCTGAATC TGCTCAACAG 
GCATCTGCTA ATAAAAGACC TCTTTATATG CAGTTCATGG ATCTACCCCC CCATCCTCCT 
CCACCATCAG TACTAGAGGA CAAACCACTT CTTGAGTTAT CTCCAAAAAT GTCTCCAGAG 
CACCTTGTAT CTGGTCCTGC AACACCAGCG ACCAGTCCTC CAGTCATATC CAATAAAACA 
TCTCCAGCTA CTCCACCAAG CACAACTTCA TCATTTTCTA TCACAACAAT TCATCACAGT 
CCTGTGAAAA ACCATAGTCC TCTGGCAGGA CAGATGCCTA CAGCAGTCAC TCCTGGACTC 
AGTCCTTCAT CAAACCAGGT TCCTGTATCC TTTCTATCTG ATGAAATGCT ACCTTCTGAA 
ACAAATAAAA TGAATTCTGT CATACTGAGT GAAGCATTTA CAACAGGAAT TCCTGTTACT 
CCACCATCTG AAAAACCCAA GGAAATTTCT AGACCACCAC ATCTTCACCA GCGTTCTGAA 
TCCTTTCCTT CACATCCTGC ATATAGCAAT GCAAAGCCCA CTCCACCAGT TAGAACAATG 
GATAGTCGAC TGGCAACTGC CATGCATTCC AACTTCAATG ATGCCATCAC TGCTAATAAC 
TACCACTCCT TCCTGAACAC CATGATGCTT CCTTCTTCTC TAGAGGATGT GCTACCCAGA 
CACAACTATT CTCATTCTTC TCATCTCAAG ACAGAAAATA TAGATGAACA GGGAGTTAAT 
TACAGACAAC CTTATTTATC CAACACCAAG CAAGAGGACC CAGCTGATGT GGGAGATTTA 
TACAGACAGC AATATATTTC TACTGGAAAG TCTGAGAATG CTGAGATAGG AAACACTTAC 
CGGCACCCAT ATCCTTCCAA TACTGAGCCA GAAAGTCTAA AGGAGCCACT AGATCCTTTT 
CTGCATCACA AGTCAGCAGT TGCTCCAAAG ACATATAGAG CAGAAACACT TCTACCACAT 
ATGCCTCCTC AAGTTTATGG TTCAAGATGT GATACTCCAT CCTCAACATT TTATGGCACC 
TACATAAATC AGGCCAAACA CCCCCGCACC AGGAACAGAC CTGATTATAT GTCCTCTATG 
AGCCCAGGTC TCCGTAGCTA CTCAGAAGAC ACATCTTCCT ACCCTACTAT TCGAAGGGTA 
CAATCTCTGC ATGTGCCTAT GCAACCTCCG CCACCTCCTA TCCGCACTGT TCCAATTTCA 
AGAACAGAGG TTCCCCCAGA TGATGATCCA GCATACTGCC CAAGACCAGT TTATCAATAC 
AAACAATGCC CCCAATTTAA CTCTCAGTCA GATTACCATG TTACCCAGCT GCAGCCTTAC 
TTTGAAAATG GACGAGTTCA TTATCGCTAC AGTCCCTATT CAGGGGGCCC AAGTTATTTT 
TCAGCAGATA GCAATTTCTA TGATATGGAC CCCTACAGCA CCTTACGTCT GCGACAACTT 
CCTCTCTACC CAAGCCGGGA CTTTGCTTCT TACACCACAA GATTGCAGCC CAAAAACACA 
TATCGGTCAC AAGGATTGCC CCCATATCCC AGAGGAACTC TACGGGAACA CAATTTTATC 
AGTAGAGATG TTCCTCCTGC ACTACCACCT GAACATCCAC GACCTCTTCA CATTTCTTGG 
GACATGGAAG ACATGGACAG ATACAGACTG CAATCCCTGC GACGAGAGAA CAGGGCACGT 
CAGAGAGCTA AAGGACCAGT AATGTCTCAG TATGATAATG TTACACCAAC CTTAGTGGAG 
GATGTAGCAG GCTTAGATGT CATACATCTC CGAAGCCGTT CTGATCCTGG TAAGACTCCT 
GGTCTTTTAA GTGTGGCTGA GAACAAAGAC GTCCGTTACC CAGGAAGGGC AGAAGGTGAT 
GAGCATACAA CATATCCACC TCCTGTTTTT GGAAATGGCC ATCAAGATAA GCCATCTCTT 
CCTCAAAAGC AAAGTGGATC AAGCCGGGTT CGAATGCAAC ATGAAATATC TACTGAGCAG 
CGGACTCAAC CTTCTCAAGA TACTTTGCAT AGGCAACCCA CAGAGGGACG AAATGGTCCT 
CCAATTCCTC CTGTGGACTA CAGCTCTAAA GGAATGCCAA ATCCCCCAGA CCCTGCATCA 
TATCATTCCT CTGCAAACAA ATATAACTTG ACACAACAGG AACCCATGCG ACTTATTCAC 
AAAGAGCTTC GGCTCCCTGA GGATATTGAG CATTCACATA CCAGGCCAGC TGACAACCAA 
CACCGTGACT GTTACAGGGA GGAGCCAGCT CAGTTTCCCC TTACTGCACC TCCTCCTAAA 
CCAGAGAGGA GCCATAGCCT TCGAGGTCAT AATCCAGATG TTTTAGAAAG AGACCCTAGT 
ATCTTCTACC CCTACCAAAC GCTTCAAGCC AAGAGACACA GCACAATTAA TGCAGTGTCA 
CAATATGATA ATCTAGCTGA TTACCATTCT ATACCCCATC ATCGAACCAC TAACCAGTCT 
ACTCCAAATG CCTTTAACCT CCCACATGGA CGGACCTATG CTACTGCCCT GGGCCAGGGT 
GCCTTTCTAG CTGCTGAACT CGGCTTGCAA AGGCCTGAGA CAAAAATCCA TGTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822582.2
CDS	343..6102
Translation	MYRIMKSSLHLEEDDFVPELPRSIHPRERPDWEETISAMARGADIPVPGEIPTMPTEIPLRSCGSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGSIQLTLTEDQNEVSRNGCESKELVFLVQIACQGRSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLSELPRSDSVKDSPELVTQMLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINVPAIAAAHVIKRYNAQAPDELSFEVGDIVSVIDMPPKELTTWWRGKHGFQVGFFPSECVELINDKVPQSMTNSVPKPVSRKHGKLITFLRTFMKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSDAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_012967128.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967128.2

ORF Insert Sequence:

ATGTACAGAA TAATGAAGTC TTCACTGCAC TTAGAGGAGG ATGATTTTGT ACCCGAGTTG 
CCAAGGAGTA TCCATCCCCG GGAGAGGCCG GACTGGGAGG AGACTATTAG TGCCATGGCA 
CGAGGGGCAG ACATCCCAGT CCCAGGGGAA ATACCGACGA TGCCTACAGA AATCCCTCTT 
CGCTCCTGTG GCAGTACTGC CAGCATGAAG GTGAAAAATG TCAAGAAGTT ATCCTTCACG 
AAAGGGCATT TTCCTAAACT TGCAGAATGT GCACATTTCC ACTATGAAAA TGTAGACTTT 
GGCAGCATAC AGCTCACACT CACAGAAGAT CAAAATGAAG TGAGCAGAAA TGGCTGTGAA 
TCCAAGGAAC TTGTTTTCCT AGTACAGATT GCTTGTCAGG GCCGTAGCTG GATAGTTAAG 
CGGAGTTATG AAGATTTTCG GGTTCTGGAT AAACACCTTC ATCTGTGCAT ATATGATAGG 
CGCTTCTCTC AGCTCTCAGA GCTGCCCCGT TCCGACTCCG TAAAGGATAG TCCAGAGTTG 
GTCACACAGA TGCTGATGGC TTACCTATCG CGGCTGTCAG CCATCGCAGG TAACAAGATC 
AATTGTGGGC CAGCTCTGAC GTGGATGGAG ATCGATAATA AAGGCAATCA TTTGCTTGTG 
CACGAAGAGT CCTCCATAAA TGTTCCAGCC ATCGCTGCTG CCCATGTCAT AAAGCGATAT 
AATGCTCAGG CCCCAGATGA ACTCTCTTTT GAGGTGGGAG ACATAGTATC TGTAATTGAC 
ATGCCACCAA AAGAACTGAC CACGTGGTGG AGAGGAAAGC ATGGATTTCA AGTGGGTTTC 
TTTCCAAGTG AATGTGTGGA ATTAATAAAT GATAAAGTCC CCCAATCAAT GACAAACTCT 
GTGCCTAAAC CAGTGTCCCG GAAGCACGGC AAGCTCATCA CATTTCTAAG AACCTTCATG 
AAGTCCAGAC CCAGCAAACA GAAACTGAAG CAGAGAGGGA TCCTGAGGGA GCGAGTGTTT 
GGTTGTGACC TTGGGGAACA TCTGCTTAAC TCTGGTCAGG ATGTCCCCCA AGTCCTCAGG 
AGCTGCACAG AGTTTATAGA AAAACATGGC ATTGTTGATG GAATCTACCG TCTGTCTGGG 
ATTGCATCTA ACATCCAGAA ACTACGTCAT GAATTTGACT CTGAACAGAT TCCAGACCTG 
ACTAAAGATG TGTACATCCA GGACATTCAC TGTGTTGGCT CTCTCTGTAA ACTCTACTTC 
CGGGAACTCC CAAACCCTCT GCTCACATAT CAGTTGTATG AAAAGTTCTC TGATGCTGTG 
TCAGCGGCTA CTGATGAAGA GAGACTGGTG AAAATTCATG ATGTTATTCA ACAGCTTCCC 
CCACCTCATT ACAGAACACT GGAGTTCCTT ATGCGCCACT TATCAAGACT TGCGACATAC 
TGCTCTATCA CCAATATGCA CACTAAAAAT TTGGCAATTG TCTGGGCCCC TAACCTGCTC 
CGGTCAAAAC AGATTGAGTC TGCATGTTTT AGCGGTACAG CAGCTTTCAT GGAAGTGCGC 
ATACAGTCTG TGGTAGTTGA ATTTATTCTA AACCATGTGG AGGTTCTCTT TAGTCCTAAA 
CTTAGTTCTG TGATAAGGGA TGGTGCTGGT CACACTTCTT TGTCTCGGCC CAAGTCACTG 
TTGGTGTCAT CTCCATCTAC AAAACTACTA AGCCTAGAGG AGGCTCAAGC AAGGACACAG 
GCCCAAATCA ATTCCCCTGT TGTGGCTGAC AGCAAGTACA TTGAGGTGGG AGAGGGACCA 
GCTGCTTTGC AGGGCAGATT CCACACTGTA ATTGACTTCC CTTCTGAAAG GAAAAGACCG 
CCAACCAAAA TGAAAAAATC TCCTGTTGGA AGCTGGCGTT CTTTTTTTAG CATAGGCAAA 
TCATCATCTT CAGCCTCCAT GTCTAAGCGG AAACTTCAGC GTAATCCAAG TGAGCCTTTG 
GAAATGAAAT CCATGGCATT TGCAGGTGGA AGAGATAGCA GCACCATGCG CTCAGCTAAA 
AGCGAGGAGT CTCTCAGTTC CTTGCATGCA GCAGATGGAG AGTCGAAGCT ATTTCGTCCA 
CGTCGTCCTC GTTCCAGCAG CGATGCTCTG TCTGCTTCAT ATAATGGGGA CCTTCTGGAT 
AGCTGCAACC GTTGCAACTC CTATGATAAT CTGCCCCGAG ACCAAGACAG TGATGGAGAT 
GAGGGTTTGA TTCATGTGCC TGCTATGCTG TCAGGTCGCT CTCCTGAGGA TGAAGAACTC 
AGCCCACCAG ACATTGGTAT GGCCAGTTTG GACTTTGACC CAATGTCTTT TCAGTGCAGC 
CCTCCCCAGC TAGAATCTGA AGGACCAGAC ATTTTTTTTC AGCTTGACAT TTGTGCTAGC 
AATGGCAAGG ATAAGGTGCA AAGTGATGCA CAAAACCTAG GCGGTGTCTC CAGCTCCGAA 
CCTCACTCTC CTTTCCAAGA GAAAGTCGTT AGTCCATTTC TATCGCCTGA CCGCAGTTCT 
AGTACAGATA AAGTATCCAA AACTACATCA TTTGCAGAAA AAGTTGTGCA GGCCTTGTCA 
CCTAAAGTGG GGCGCAAAGC AGTTCGATCT CCTCCATTAA CCATCTCAGA GCCTGTCACC 
ATCTCTCTCC CTAGTCGGGT ATCGGAAATG ATTGGAAGTA TGCAAGGCAA TACTGCTGCT 
TCCCAGTCTT TAATTTGGTA CAGAGAAAAC AGTTTAAATT CTAGAGAAAC TGAAATGAAT 
TATGTTTGTA AATCGGTGGA TGCCAACCTT CCATCCTTAT TGCCAGCAAC AGATCCATGG 
TCTACAGTTA CTCCTGTAAT TACTCAATGG AACCCAGATG GATTACAGAG AGAAGACAAA 
GCAGGACCAT TTACTCATGG CTTTCCCCAG CCAAATAGTG ACTTGGAACA AGTAGAAAAC 
TTCAACCTGG AACTTAGCAC AACAAATGTT GCTGCAGCAT ATCAGAAAGA AGATACAGAA 
TTGTCGAGAG CAAATCAGAA CAAATCCCAC TCTTCTGTTT CTCCCCCTCA AGGAGCTTCT 
GCTTCAGAAT CTCCACAGGA GCTAGCCCCT GTCAGTAGTG TTTCTATCAT TCCACCTCCA 
CCTCCTCCAA AGAATCCTGC ACGCATGCTG GCATTAGCAC TGGCTGAATC TGCTCAACAG 
GCATCTGCTA ATAAAAGACC TCTTTATATG CAGTTCATGG ATCTACCCCC CCATCCTCCT 
CCACCATCAG TACTAGAGGA CAAACCACTT CTTGAGTTAT CTCCAAAAAT GTCTCCAGAG 
CACCTTGTAT CTGGTCCTGC AACACCAGCG ACCAGTCCTC CAGTCATATC CAATAAAACA 
TCTCCAGCTA CTCCACCAAG CACAACTTCA TCATTTTCTA TCACAACAAT TCATCACAGT 
CCTGTGAAAA ACCATAGTCC TCTGGCAGGA CAGATGCCTA CAGCAGTCAC TCCTGGACTC 
AGTCCTTCAT CAAACCAGGT TCCTGTATCC TTTCTATCTG ATGAAATGCT ACCTTCTGAA 
ACAAATAAAA TGAATTCTGT CATACTGAGT GAAGCATTTA CAACAGGAAT TCCTGTTACT 
CCACCATCTG AAAAACCCAA GGAAATTTCT AGACCACCAC ATCTTCACCA GCGTTCTGAA 
TCCTTTCCTT CACATCCTGC ATATAGCAAT GCAAAGCCCA CTCCACCAGT TAGAACAATG 
GATAGTCGAC TGGCAACTGC CATGCATTCC AACTTCAATG ATGCCATCAC TGCTAATAAC 
TACCACTCCT TCCTGAACAC CATGATGCTT CCTTCTTCTC TAGAGGATGT GCTACCCAGA 
CACAACTATT CTCATTCTTC TCATCTCAAG ACAGAAAATA TAGATGAACA GGGAGTTAAT 
TACAGACAAC CTTATTTATC CAACACCAAG CAAGAGGACC CAGCTGATGT GGGAGATTTA 
TACAGACAGC AATATATTTC TACTGGAAAG TCTGAGAATG CTGAGATAGG AAACACTTAC 
CGGCACCCAT ATCCTTCCAA TACTGAGCCA GAAAGTCTAA AGGAGCCACT AGATCCTTTT 
CTGCATCACA AGTCAGCAGT TGCTCCAAAG ACATATAGAG CAGAAACACT TCTACCACAT 
ATGCCTCCTC AAGTTTATGG TTCAAGATGT GATACTCCAT CCTCAACATT TTATGGCACC 
TACATAAATC AGGCCAAACA CCCCCGCACC AGGAACAGAC CTGATTATAT GTCCTCTATG 
AGCCCAGGTC TCCGTAGCTA CTCAGAAGAC ACATCTTCCT ACCCTACTAT TCGAAGGGTA 
CAATCTCTGC ATGTGCCTAT GCAACCTCCG CCACCTCCTA TCCGCACTGT TCCAATTTCA 
AGAACAGAGG TTCCCCCAGA TGATGATCCA GCATACTGCC CAAGACCAGT TTATCAATAC 
AAACAATGCC CCCAATTTAA CTCTCAGTCA GATTACCATG TTACCCAGCT GCAGCCTTAC 
TTTGAAAATG GACGAGTTCA TTATCGCTAC AGTCCCTATT CAGGGGGCCC AAGTTATTTT 
TCAGCAGATA GCAATTTCTA TGATATGGAC CCCTACAGCA CCTTACGTCT GCGACAACTT 
CCTCTCTACC CAAGCCGGGA CTTTGCTTCT TACACCACAA GATTGCAGCC CAAAAACACA 
TATCGGTCAC AAGGATTGCC CCCATATCCC AGAGGAACTC TACGGGAACA CAATTTTATC 
AGTAGAGATG TTCCTCCTGC ACTACCACCT GAACATCCAC GACCTCTTCA CATTTCTTGG 
GACATGGAAG ACATGGACAG ATACAGACTG CAATCCCTGC GACGAGAGAA CAGGGCACGT 
CAGAGAGCTA AAGGACCAGT AATGTCTCAG TATGATAATG TTACACCAAC CTTAGTGGAG 
GATGTAGCAG GCTTAGATGT CATACATCTC CGAAGCCGTT CTGATCCTGG TAAGACTCCT 
GGTCTTTTAA GTGTGGCTGA GAACAAAGAC GTCCGTTACC CAGGAAGGGC AGAAGGTGAT 
GAGCATACAA CATATCCACC TCCTGTTTTT GGAAATGGCC ATCAAGATAA GCCATCTCTT 
CCTCAAAAGC AAAGTGGATC AAGCCGGGTT CGAATGCAAC ATGAAATATC TACTGAGCAG 
CGGACTCAAC CTTCTCAAGA TACTTTGCAT AGGCAACCCA CAGAGGGACG AAATGGTCCT 
CCAATTCCTC CTGTGGACTA CAGCTCTAAA GGAATGCCAA ATCCCCCAGA CCCTGCATCA 
TATCATTCCT CTGCAAACAA ATATAACTTG ACACAACAGG AACCCATGCG ACTTATTCAC 
AAAGAGCTTC GGCTCCCTGA GGATATTGAG CATTCACATA CCAGGCCAGC TGACAACCAA 
CACCGTGACT GTTACAGGGA GGAGCCAGCT CAGTTTCCCC TTACTGCACC TCCTCCTAAA 
CCAGAGAGGA GCCATAGCCT TCGAGGTCAT AATCCAGATG TTTTAGAAAG AGACCCTAGT 
ATCTTCTACC CCTACCAAAC GCTTCAAGCC AAGAGACACA GCACAATTAA TGCAGTGTCA 
CAATATGATA ATCTAGCTGA TTACCATTCT ATACCCCATC ATCGAACCAC TAACCAGTCT 
ACTCCAAATG CCTTTAACCT CCCACATGGA CGGACCTATG CTACTGCCCT GGGCCAGGGT 
GCCTTTCTAG CTGCTGAACT CGGCTTGCAA AGGCCTGAGA CAAAAATCCA TGTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_018095931.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951420.2 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X10, mRNA.

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CloneID	OXa61967
Clone ID Related Accession (Same CDS sequence)	XM_018095931.2
Accession Version	XM_018095931.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018095931.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATGG CTTACCTATC GCGGCTGTCA GCCATCGCAG GTAACAAGAT CAATTGTGGG 
CCAGCTCTGA CGTGGATGGA GATCGATAAT AAAGGCAATC ATTTGCTTGT GCACGAAGAG 
TCCTCCATAA ATGTTCCAGC CATCGCTGCT GCCCATGTCA TAAAGCGATA TAATGCTCAG 
GCCCCAGATG AACTCTCTTT TGAGGTGGGA GACATAGTAT CTGTAATTGA CATGCCACCA 
AAAGAACTGA CCACGTGGTG GAGAGGAAAG CATGGATTTC AAGTGGGTTT CTTTCCAAGT 
GAATGTGTGG AATTAATAAA TGATAAAGTC CCCCAATCAA TGACAAACTC TGTGCCTAAA 
CCAGATTTGG AGATGGATAG TTTAAGGCCT GGAACATCGT GCTGTTCTGA GGTATTAAGT 
CACTCGCAGC TAAGCTCAGT GTCCCGGAAG CACGGCAAGC TCATCACATT TCTAAGAACC 
TTCATGAAGT CCAGACCCAG CAAACAGAAA CTGAAGCAGA GAGGGATCCT GAGGGAGCGA 
GTGTTTGGTT GTGACCTTGG GGAACATCTG CTTAACTCTG GTCAGGATGT CCCCCAAGTC 
CTCAGGAGCT GCACAGAGTT TATAGAAAAA CATGGCATTG TTGATGGAAT CTACCGTCTG 
TCTGGGATTG CATCTAACAT CCAGAAACTA CGTCATGAAT TTGACTCTGA ACAGATTCCA 
GACCTGACTA AAGATGTGTA CATCCAGGAC ATTCACTGTG TTGGCTCTCT CTGTAAACTC 
TACTTCCGGG AACTCCCAAA CCCTCTGCTC ACATATCAGT TGTATGAAAA GTTCTCTGAT 
GCTGTGTCAG CGGCTACTGA TGAAGAGAGA CTGGTGAAAA TTCATGATGT TATTCAACAG 
CTTCCCCCAC CTCATTACAG AACACTGGAG TTCCTTATGC GCCACTTATC AAGACTTGCG 
ACATACTGCT CTATCACCAA TATGCACACT AAAAATTTGG CAATTGTCTG GGCCCCTAAC 
CTGCTCCGGT CAAAACAGAT TGAGTCTGCA TGTTTTAGCG GTACAGCAGC TTTCATGGAA 
GTGCGCATAC AGTCTGTGGT AGTTGAATTT ATTCTAAACC ATGTGGAGGT TCTCTTTAGT 
CCTAAACTTA GTTCTGTGAT AAGGGATGGT GCTGGTCACA CTTCTTTGTC TCGGCCCAAG 
TCACTGTTGG TGTCATCTCC ATCTACAAAA CTACTAAGCC TAGAGGAGGC TCAAGCAAGG 
ACACAGGCCC AAATCAATTC CCCTGTTGTG GCTGACAGCA AGTACATTGA GGTGGGAGAG 
GGACCAGCTG CTTTGCAGGG CAGATTCCAC ACTGTAATTG ACTTCCCTTC TGAAAGGAAA 
AGACCGCCAA CCAAAATGAA AAAATCTCCT GTTGGAAGCT GGCGTTCTTT TTTTAGCATA 
GGCAAATCAT CATCTTCAGC CTCCATGTCT AAGCGGAAAC TTCAGCGTAA TCCAAGTGAG 
CCTTTGGAAA TGAAATCCAT GGCATTTGCA GGTGGAAGAG ATAGCAGCAC CATGCGCTCA 
GCTAAAAGCG AGGAGTCTCT CAGTTCCTTG CATGCAGCAG ATGGAGAGTC GAAGCTATTT 
CGTCCACGTC GTCCTCGTTC CAGCAGCGAT GCTCTGTCTG CTTCATATAA TGGGGACCTT 
CTGGATAGCT GCAACCGTTG CAACTCCTAT GATAATCTGC CCCGAGACCA AGACAGTGAT 
GGAGATGAGG GTTTGATTCA TGTGCCTGCT ATGCTGTCAG GTCGCTCTCC TGAGGATGAA 
GAACTCAGCC CACCAGACAT TGGTATGGCC AGTTTGGACT TTGACCCAAT GTCTTTTCAG 
TGCAGCCCTC CCCAGCTAGA ATCTGAAGGA CCAGACATTT TTTTTCAGCT TGACATTTGT 
GCTAGCAATG GCAAGGATAA GGTGCAAAGT GATGCACAAA ACCTAGGCGG TGTCTCCAGC 
TCCGAACCTC ACTCTCCTTT CCAAGAGAAA GTCGTTAGTC CATTTCTATC GCCTGACCGC 
AGTTCTAGTA CAGATAAAGT ATCCAAAACT ACATCATTTG CAGAAAAAGT TGTGCAGGCC 
TTGTCACCTA AAGTGGGGCG CAAAGCAGTT CGATCTCCTC CATTAACCAT CTCAGAGCCT 
GTCACCATCT CTCTCCCTAG TCGGGTATCG GAAATGATTG GAAGTATGCA AGGCAATACT 
GCTGCTTCCC AGTCTTTAAT TTGGTACAGA GAAAACAGTT TAAATTCTAG AGAAACTGAA 
ATGAATTATG TTTGTAAATC GGTGGATGCC AACCTTCCAT CCTTATTGCC AGCAACAGAT 
CCATGGTCTA CAGTTACTCC TGTAATTACT CAATGGAACC CAGATGGATT ACAGAGAGAA 
GACAAAGCAG GACCATTTAC TCATGGCTTT CCCCAGCCAA ATAGTGACTT GGAACAAGTA 
GAAAACTTCA ACCTGGAACT TAGCACAACA AATGTTGCTG CAGCATATCA GAAAGAAGAT 
ACAGAATTGT CGAGAGCAAA TCAGAACAAA TCCCACTCTT CTGTTTCTCC CCCTCAAGGA 
GCTTCTGCTT CAGAATCTCC ACAGGAGCTA GCCCCTGTCA GTAGTGTTTC TATCATTCCA 
CCTCCACCTC CTCCAAAGAA TCCTGCACGC ATGCTGGCAT TAGCACTGGC TGAATCTGCT 
CAACAGGCAT CTGCTAATAA AAGACCTCTT TATATGCAGT TCATGGATCT ACCCCCCCAT 
CCTCCTCCAC CATCAGTACT AGAGGACAAA CCACTTCTTG AGTTATCTCC AAAAATGTCT 
CCAGAGCACC TTGTATCTGG TCCTGCAACA CCAGCGACCA GTCCTCCAGT CATATCCAAT 
AAAACATCTC CAGCTACTCC ACCAAGCACA ACTTCATCAT TTTCTATCAC AACAATTCAT 
CACAGTCCTG TGAAAAACCA TAGTCCTCTG GCAGGACAGA TGCCTACAGC AGTCACTCCT 
GGACTCAGTC CTTCATCAAA CCAGGTTCCT GTATCCTTTC TATCTGATGA AATGCTACCT 
TCTGAAACAA ATAAAATGAA TTCTGTCATA CTGAGTGAAG CATTTACAAC AGGAATTCCT 
GTTACTCCAC CATCTGAAAA ACCCAAGGAA ATTTCTAGAC CACCACATCT TCACCAGCGT 
TCTGAATCCT TTCCTTCACA TCCTGCATAT AGCAATGCAA AGCCCACTCC ACCAGTTAGA 
ACAATGGATA GTCGACTGGC AACTGCCATG CATTCCAACT TCAATGATGC CATCACTGCT 
AATAACTACC ACTCCTTCCT GAACACCATG ATGCTTCCTT CTTCTCTAGA GGATGTGCTA 
CCCAGACACA ACTATTCTCA TTCTTCTCAT CTCAAGACAG AAAATATAGA TGAACAGGGA 
GTTAATTACA GACAACCTTA TTTATCCAAC ACCAAGCAAG AGGACCCAGC TGATGTGGGA 
GATTTATACA GACAGCAATA TATTTCTACT GGAAAGTCTG AGAATGCTGA GATAGGAAAC 
ACTTACCGGC ACCCATATCC TTCCAATACT GAGCCAGAAA GTCTAAAGGA GCCACTAGAT 
CCTTTTCTGC ATCACAAGTC AGCAGTTGCT CCAAAGACAT ATAGAGCAGA AACACTTCTA 
CCACATATGC CTCCTCAAGT TTATGGTTCA AGATGTGATA CTCCATCCTC AACATTTTAT 
GGCACCTACA TAAATCAGGC CAAACACCCC CGCACCAGGA ACAGACCTGA TTATATGTCC 
TCTATGAGCC CAGGTCTCCG TAGCTACTCA GAAGACACAT CTTCCTACCC TACTATTCGA 
AGGGTACAAT CTCTGCATGT GCCTATGCAA CCTCCGCCAC CTCCTATCCG CACTGTTCCA 
ATTTCAAGAA CAGAGGTTCC CCCAGATGAT GATCCAGCAT ACTGCCCAAG ACCAGTTTAT 
CAATACAAAC AATGCCCCCA ATTTAACTCT CAGTCAGATT ACCATGTTAC CCAGCTGCAG 
CCTTACTTTG AAAATGGACG AGTTCATTAT CGCTACAGTC CCTATTCAGG GGGCCCAAGT 
TATTTTTCAG CAGATAGCAA TTTCTATGAT ATGGACCCCT ACAGCACCTT ACGTCTGCGA 
CAACTTCCTC TCTACCCAAG CCGGGACTTT GCTTCTTACA CCACAAGATT GCAGCCCAAA 
AACACATATC GGTCACAAGG ATTGCCCCCA TATCCCAGAG GAACTCTACG GGAACACAAT 
TTTATCAGTA GAGATGTTCC TCCTGCACTA CCACCTGAAC ATCCACGACC TCTTCACATT 
TCTTGGGACA TGGAAGACAT GGACAGATAC AGACTGCAAT CCCTGCGACG AGAGAACAGG 
GCACGTCAGA GAGCTAAAGG ACCAGTAATG TCTCAGTATG ATAATGTTAC ACCAACCTTA 
GTGGAGGATG TAGCAGGCTT AGATGTCATA CATCTCCGAA GCCGTTCTGA TCCTGGTAAG 
ACTCCTGGTC TTTTAAGTGT GGCTGAGAAC AAAGACGTCC GTTACCCAGG AAGGGCAGAA 
GGTGATGAGC ATACAACATA TCCACCTCCT GTTTTTGGAA ATGGCCATCA AGATAAGCCA 
TCTCTTCCTC AAAAGCAAAG TGGATCAAGC CGGGTTCGAA TGCAACATGA AATATCTACT 
GAGCAGCGGA CTCAACCTTC TCAAGATACT TTGCATAGGC AACCCACAGA GGGACGAAAT 
GGTCCTCCAA TTCCTCCTGT GGACTACAGC TCTAAAGGAA TGCCAAATCC CCCAGACCCT 
GCATCATATC ATTCCTCTGC AAACAAATAT AACTTGACAC AACAGGAACC CATGCGACTT 
ATTCACAAAG AGCTTCGGCT CCCTGAGGAT ATTGAGCATT CACATACCAG GCCAGCTGAC 
AACCAACACC GTGACTGTTA CAGGGAGGAG CCAGCTCAGT TTCCCCTTAC TGCACCTCCT 
CCTAAACCAG AGAGGAGCCA TAGCCTTCGA GGTCATAATC CAGATGTTTT AGAAAGAGAC 
CCTAGTATCT TCTACCCCTA CCAAACGCTT CAAGCCAAGA GACACAGCAC AATTAATGCA 
GTGTCACAAT ATGATAATCT AGCTGATTAC CATTCTATAC CCCATCATCG AACCACTAAC 
CAGTCTACTC CAAATGCCTT TAACCTCCCA CATGGACGGA CCTATGCTAC TGCCCTGGGC 
CAGGGTGCCT TTCTAGCTGC TGAACTCGGC TTGCAAAGGC CTGAGACAAA AATCCATGTG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951420.2
CDS	597..5882
Translation	MLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINVPAIAAAHVIKRYNAQAPDELSFEVGDIVSVIDMPPKELTTWWRGKHGFQVGFFPSECVELINDKVPQSMTNSVPKPDLEMDSLRPGTSCCSEVLSHSQLSSVSRKHGKLITFLRTFMKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSDAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_018095931.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095931.2

ORF Insert Sequence:

ATGCTGATGG CTTACCTATC GCGGCTGTCA GCCATCGCAG GTAACAAGAT CAATTGTGGG 
CCAGCTCTGA CGTGGATGGA GATCGATAAT AAAGGCAATC ATTTGCTTGT GCACGAAGAG 
TCCTCCATAA ATGTTCCAGC CATCGCTGCT GCCCATGTCA TAAAGCGATA TAATGCTCAG 
GCCCCAGATG AACTCTCTTT TGAGGTGGGA GACATAGTAT CTGTAATTGA CATGCCACCA 
AAAGAACTGA CCACGTGGTG GAGAGGAAAG CATGGATTTC AAGTGGGTTT CTTTCCAAGT 
GAATGTGTGG AATTAATAAA TGATAAAGTC CCCCAATCAA TGACAAACTC TGTGCCTAAA 
CCAGATTTGG AGATGGATAG TTTAAGGCCT GGAACATCGT GCTGTTCTGA GGTATTAAGT 
CACTCGCAGC TAAGCTCAGT GTCCCGGAAG CACGGCAAGC TCATCACATT TCTAAGAACC 
TTCATGAAGT CCAGACCCAG CAAACAGAAA CTGAAGCAGA GAGGGATCCT GAGGGAGCGA 
GTGTTTGGTT GTGACCTTGG GGAACATCTG CTTAACTCTG GTCAGGATGT CCCCCAAGTC 
CTCAGGAGCT GCACAGAGTT TATAGAAAAA CATGGCATTG TTGATGGAAT CTACCGTCTG 
TCTGGGATTG CATCTAACAT CCAGAAACTA CGTCATGAAT TTGACTCTGA ACAGATTCCA 
GACCTGACTA AAGATGTGTA CATCCAGGAC ATTCACTGTG TTGGCTCTCT CTGTAAACTC 
TACTTCCGGG AACTCCCAAA CCCTCTGCTC ACATATCAGT TGTATGAAAA GTTCTCTGAT 
GCTGTGTCAG CGGCTACTGA TGAAGAGAGA CTGGTGAAAA TTCATGATGT TATTCAACAG 
CTTCCCCCAC CTCATTACAG AACACTGGAG TTCCTTATGC GCCACTTATC AAGACTTGCG 
ACATACTGCT CTATCACCAA TATGCACACT AAAAATTTGG CAATTGTCTG GGCCCCTAAC 
CTGCTCCGGT CAAAACAGAT TGAGTCTGCA TGTTTTAGCG GTACAGCAGC TTTCATGGAA 
GTGCGCATAC AGTCTGTGGT AGTTGAATTT ATTCTAAACC ATGTGGAGGT TCTCTTTAGT 
CCTAAACTTA GTTCTGTGAT AAGGGATGGT GCTGGTCACA CTTCTTTGTC TCGGCCCAAG 
TCACTGTTGG TGTCATCTCC ATCTACAAAA CTACTAAGCC TAGAGGAGGC TCAAGCAAGG 
ACACAGGCCC AAATCAATTC CCCTGTTGTG GCTGACAGCA AGTACATTGA GGTGGGAGAG 
GGACCAGCTG CTTTGCAGGG CAGATTCCAC ACTGTAATTG ACTTCCCTTC TGAAAGGAAA 
AGACCGCCAA CCAAAATGAA AAAATCTCCT GTTGGAAGCT GGCGTTCTTT TTTTAGCATA 
GGCAAATCAT CATCTTCAGC CTCCATGTCT AAGCGGAAAC TTCAGCGTAA TCCAAGTGAG 
CCTTTGGAAA TGAAATCCAT GGCATTTGCA GGTGGAAGAG ATAGCAGCAC CATGCGCTCA 
GCTAAAAGCG AGGAGTCTCT CAGTTCCTTG CATGCAGCAG ATGGAGAGTC GAAGCTATTT 
CGTCCACGTC GTCCTCGTTC CAGCAGCGAT GCTCTGTCTG CTTCATATAA TGGGGACCTT 
CTGGATAGCT GCAACCGTTG CAACTCCTAT GATAATCTGC CCCGAGACCA AGACAGTGAT 
GGAGATGAGG GTTTGATTCA TGTGCCTGCT ATGCTGTCAG GTCGCTCTCC TGAGGATGAA 
GAACTCAGCC CACCAGACAT TGGTATGGCC AGTTTGGACT TTGACCCAAT GTCTTTTCAG 
TGCAGCCCTC CCCAGCTAGA ATCTGAAGGA CCAGACATTT TTTTTCAGCT TGACATTTGT 
GCTAGCAATG GCAAGGATAA GGTGCAAAGT GATGCACAAA ACCTAGGCGG TGTCTCCAGC 
TCCGAACCTC ACTCTCCTTT CCAAGAGAAA GTCGTTAGTC CATTTCTATC GCCTGACCGC 
AGTTCTAGTA CAGATAAAGT ATCCAAAACT ACATCATTTG CAGAAAAAGT TGTGCAGGCC 
TTGTCACCTA AAGTGGGGCG CAAAGCAGTT CGATCTCCTC CATTAACCAT CTCAGAGCCT 
GTCACCATCT CTCTCCCTAG TCGGGTATCG GAAATGATTG GAAGTATGCA AGGCAATACT 
GCTGCTTCCC AGTCTTTAAT TTGGTACAGA GAAAACAGTT TAAATTCTAG AGAAACTGAA 
ATGAATTATG TTTGTAAATC GGTGGATGCC AACCTTCCAT CCTTATTGCC AGCAACAGAT 
CCATGGTCTA CAGTTACTCC TGTAATTACT CAATGGAACC CAGATGGATT ACAGAGAGAA 
GACAAAGCAG GACCATTTAC TCATGGCTTT CCCCAGCCAA ATAGTGACTT GGAACAAGTA 
GAAAACTTCA ACCTGGAACT TAGCACAACA AATGTTGCTG CAGCATATCA GAAAGAAGAT 
ACAGAATTGT CGAGAGCAAA TCAGAACAAA TCCCACTCTT CTGTTTCTCC CCCTCAAGGA 
GCTTCTGCTT CAGAATCTCC ACAGGAGCTA GCCCCTGTCA GTAGTGTTTC TATCATTCCA 
CCTCCACCTC CTCCAAAGAA TCCTGCACGC ATGCTGGCAT TAGCACTGGC TGAATCTGCT 
CAACAGGCAT CTGCTAATAA AAGACCTCTT TATATGCAGT TCATGGATCT ACCCCCCCAT 
CCTCCTCCAC CATCAGTACT AGAGGACAAA CCACTTCTTG AGTTATCTCC AAAAATGTCT 
CCAGAGCACC TTGTATCTGG TCCTGCAACA CCAGCGACCA GTCCTCCAGT CATATCCAAT 
AAAACATCTC CAGCTACTCC ACCAAGCACA ACTTCATCAT TTTCTATCAC AACAATTCAT 
CACAGTCCTG TGAAAAACCA TAGTCCTCTG GCAGGACAGA TGCCTACAGC AGTCACTCCT 
GGACTCAGTC CTTCATCAAA CCAGGTTCCT GTATCCTTTC TATCTGATGA AATGCTACCT 
TCTGAAACAA ATAAAATGAA TTCTGTCATA CTGAGTGAAG CATTTACAAC AGGAATTCCT 
GTTACTCCAC CATCTGAAAA ACCCAAGGAA ATTTCTAGAC CACCACATCT TCACCAGCGT 
TCTGAATCCT TTCCTTCACA TCCTGCATAT AGCAATGCAA AGCCCACTCC ACCAGTTAGA 
ACAATGGATA GTCGACTGGC AACTGCCATG CATTCCAACT TCAATGATGC CATCACTGCT 
AATAACTACC ACTCCTTCCT GAACACCATG ATGCTTCCTT CTTCTCTAGA GGATGTGCTA 
CCCAGACACA ACTATTCTCA TTCTTCTCAT CTCAAGACAG AAAATATAGA TGAACAGGGA 
GTTAATTACA GACAACCTTA TTTATCCAAC ACCAAGCAAG AGGACCCAGC TGATGTGGGA 
GATTTATACA GACAGCAATA TATTTCTACT GGAAAGTCTG AGAATGCTGA GATAGGAAAC 
ACTTACCGGC ACCCATATCC TTCCAATACT GAGCCAGAAA GTCTAAAGGA GCCACTAGAT 
CCTTTTCTGC ATCACAAGTC AGCAGTTGCT CCAAAGACAT ATAGAGCAGA AACACTTCTA 
CCACATATGC CTCCTCAAGT TTATGGTTCA AGATGTGATA CTCCATCCTC AACATTTTAT 
GGCACCTACA TAAATCAGGC CAAACACCCC CGCACCAGGA ACAGACCTGA TTATATGTCC 
TCTATGAGCC CAGGTCTCCG TAGCTACTCA GAAGACACAT CTTCCTACCC TACTATTCGA 
AGGGTACAAT CTCTGCATGT GCCTATGCAA CCTCCGCCAC CTCCTATCCG CACTGTTCCA 
ATTTCAAGAA CAGAGGTTCC CCCAGATGAT GATCCAGCAT ACTGCCCAAG ACCAGTTTAT 
CAATACAAAC AATGCCCCCA ATTTAACTCT CAGTCAGATT ACCATGTTAC CCAGCTGCAG 
CCTTACTTTG AAAATGGACG AGTTCATTAT CGCTACAGTC CCTATTCAGG GGGCCCAAGT 
TATTTTTCAG CAGATAGCAA TTTCTATGAT ATGGACCCCT ACAGCACCTT ACGTCTGCGA 
CAACTTCCTC TCTACCCAAG CCGGGACTTT GCTTCTTACA CCACAAGATT GCAGCCCAAA 
AACACATATC GGTCACAAGG ATTGCCCCCA TATCCCAGAG GAACTCTACG GGAACACAAT 
TTTATCAGTA GAGATGTTCC TCCTGCACTA CCACCTGAAC ATCCACGACC TCTTCACATT 
TCTTGGGACA TGGAAGACAT GGACAGATAC AGACTGCAAT CCCTGCGACG AGAGAACAGG 
GCACGTCAGA GAGCTAAAGG ACCAGTAATG TCTCAGTATG ATAATGTTAC ACCAACCTTA 
GTGGAGGATG TAGCAGGCTT AGATGTCATA CATCTCCGAA GCCGTTCTGA TCCTGGTAAG 
ACTCCTGGTC TTTTAAGTGT GGCTGAGAAC AAAGACGTCC GTTACCCAGG AAGGGCAGAA 
GGTGATGAGC ATACAACATA TCCACCTCCT GTTTTTGGAA ATGGCCATCA AGATAAGCCA 
TCTCTTCCTC AAAAGCAAAG TGGATCAAGC CGGGTTCGAA TGCAACATGA AATATCTACT 
GAGCAGCGGA CTCAACCTTC TCAAGATACT TTGCATAGGC AACCCACAGA GGGACGAAAT 
GGTCCTCCAA TTCCTCCTGT GGACTACAGC TCTAAAGGAA TGCCAAATCC CCCAGACCCT 
GCATCATATC ATTCCTCTGC AAACAAATAT AACTTGACAC AACAGGAACC CATGCGACTT 
ATTCACAAAG AGCTTCGGCT CCCTGAGGAT ATTGAGCATT CACATACCAG GCCAGCTGAC 
AACCAACACC GTGACTGTTA CAGGGAGGAG CCAGCTCAGT TTCCCCTTAC TGCACCTCCT 
CCTAAACCAG AGAGGAGCCA TAGCCTTCGA GGTCATAATC CAGATGTTTT AGAAAGAGAC 
CCTAGTATCT TCTACCCCTA CCAAACGCTT CAAGCCAAGA GACACAGCAC AATTAATGCA 
GTGTCACAAT ATGATAATCT AGCTGATTAC CATTCTATAC CCCATCATCG AACCACTAAC 
CAGTCTACTC CAAATGCCTT TAACCTCCCA CATGGACGGA CCTATGCTAC TGCCCTGGGC 
CAGGGTGCCT TTCTAGCTGC TGAACTCGGC TTGCAAAGGC CTGAGACAAA AATCCATGTG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_004916170.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916227.2 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X12, mRNA.

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CloneID	OXa61968
Clone ID Related Accession (Same CDS sequence)	XM_004916170.4 , XM_004916169.4
Accession Version	XM_004916170.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916170.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCCA GACCCAGCAA ACAGAAACTG AAGCAGAGAG GGATCCTGAG GGAGCGAGTG 
TTTGGTTGTG ACCTTGGGGA ACATCTGCTT AACTCTGGTC AGGATGTCCC CCAAGTCCTC 
AGGAGCTGCA CAGAGTTTAT AGAAAAACAT GGCATTGTTG ATGGAATCTA CCGTCTGTCT 
GGGATTGCAT CTAACATCCA GAAACTACGT CATGAATTTG ACTCTGAACA GATTCCAGAC 
CTGACTAAAG ATGTGTACAT CCAGGACATT CACTGTGTTG GCTCTCTCTG TAAACTCTAC 
TTCCGGGAAC TCCCAAACCC TCTGCTCACA TATCAGTTGT ATGAAAAGTT CTCTGATGCT 
GTGTCAGCGG CTACTGATGA AGAGAGACTG GTGAAAATTC ATGATGTTAT TCAACAGCTT 
CCCCCACCTC ATTACAGAAC ACTGGAGTTC CTTATGCGCC ACTTATCAAG ACTTGCGACA 
TACTGCTCTA TCACCAATAT GCACACTAAA AATTTGGCAA TTGTCTGGGC CCCTAACCTG 
CTCCGGTCAA AACAGATTGA GTCTGCATGT TTTAGCGGTA CAGCAGCTTT CATGGAAGTG 
CGCATACAGT CTGTGGTAGT TGAATTTATT CTAAACCATG TGGAGGTTCT CTTTAGTCCT 
AAACTTAGTT CTGTGATAAG GGATGGTGCT GGTCACACTT CTTTGTCTCG GCCCAAGTCA 
CTGTTGGTGT CATCTCCATC TACAAAACTA CTAAGCCTAG AGGAGGCTCA AGCAAGGACA 
CAGGCCCAAA TCAATTCCCC TGTTGTGGCT GACAGCAAGT ACATTGAGGT GGGAGAGGGA 
CCAGCTGCTT TGCAGGGCAG ATTCCACACT GTAATTGACT TCCCTTCTGA AAGGAAAAGA 
CCGCCAACCA AAATGAAAAA ATCTCCTGTT GGAAGCTGGC GTTCTTTTTT TAGCATAGGC 
AAATCATCAT CTTCAGCCTC CATGTCTAAG CGGAAACTTC AGCGTAATCC AAGTGAGCCT 
TTGGAAATGA AATCCATGGC ATTTGCAGGT GGAAGAGATA GCAGCACCAT GCGCTCAGCT 
AAAAGCGAGG AGTCTCTCAG TTCCTTGCAT GCAGCAGATG GAGAGTCGAA GCTATTTCGT 
CCACGTCGTC CTCGTTCCAG CAGCGATGCT CTGTCTGCTT CATATAATGG GGACCTTCTG 
GATAGCTGCA ACCGTTGCAA CTCCTATGAT AATCTGCCCC GAGACCAAGA CAGTGATGGA 
GATGAGGGTT TGATTCATGT GCCTGCTATG CTGTCAGGTC GCTCTCCTGA GGATGAAGAA 
CTCAGCCCAC CAGACATTGG TATGGCCAGT TTGGACTTTG ACCCAATGTC TTTTCAGTGC 
AGCCCTCCCC AGCTAGAATC TGAAGGACCA GACATTTTTT TTCAGCTTGA CATTTGTGCT 
AGCAATGGCA AGGATAAGGT GCAAAGTGAT GCACAAAACC TAGGCGGTGT CTCCAGCTCC 
GAACCTCACT CTCCTTTCCA AGAGAAAGTC GTTAGTCCAT TTCTATCGCC TGACCGCAGT 
TCTAGTACAG ATAAAGTATC CAAAACTACA TCATTTGCAG AAAAAGTTGT GCAGGCCTTG 
TCACCTAAAG TGGGGCGCAA AGCAGTTCGA TCTCCTCCAT TAACCATCTC AGAGCCTGTC 
ACCATCTCTC TCCCTAGTCG GGTATCGGAA ATGATTGGAA GTATGCAAGG CAATACTGCT 
GCTTCCCAGT CTTTAATTTG GTACAGAGAA AACAGTTTAA ATTCTAGAGA AACTGAAATG 
AATTATGTTT GTAAATCGGT GGATGCCAAC CTTCCATCCT TATTGCCAGC AACAGATCCA 
TGGTCTACAG TTACTCCTGT AATTACTCAA TGGAACCCAG ATGGATTACA GAGAGAAGAC 
AAAGCAGGAC CATTTACTCA TGGCTTTCCC CAGCCAAATA GTGACTTGGA ACAAGTAGAA 
AACTTCAACC TGGAACTTAG CACAACAAAT GTTGCTGCAG CATATCAGAA AGAAGATACA 
GAATTGTCGA GAGCAAATCA GAACAAATCC CACTCTTCTG TTTCTCCCCC TCAAGGAGCT 
TCTGCTTCAG AATCTCCACA GGAGCTAGCC CCTGTCAGTA GTGTTTCTAT CATTCCACCT 
CCACCTCCTC CAAAGAATCC TGCACGCATG CTGGCATTAG CACTGGCTGA ATCTGCTCAA 
CAGGCATCTG CTAATAAAAG ACCTCTTTAT ATGCAGTTCA TGGATCTACC CCCCCATCCT 
CCTCCACCAT CAGTACTAGA GGACAAACCA CTTCTTGAGT TATCTCCAAA AATGTCTCCA 
GAGCACCTTG TATCTGGTCC TGCAACACCA GCGACCAGTC CTCCAGTCAT ATCCAATAAA 
ACATCTCCAG CTACTCCACC AAGCACAACT TCATCATTTT CTATCACAAC AATTCATCAC 
AGTCCTGTGA AAAACCATAG TCCTCTGGCA GGACAGATGC CTACAGCAGT CACTCCTGGA 
CTCAGTCCTT CATCAAACCA GGTTCCTGTA TCCTTTCTAT CTGATGAAAT GCTACCTTCT 
GAAACAAATA AAATGAATTC TGTCATACTG AGTGAAGCAT TTACAACAGG AATTCCTGTT 
ACTCCACCAT CTGAAAAACC CAAGGAAATT TCTAGACCAC CACATCTTCA CCAGCGTTCT 
GAATCCTTTC CTTCACATCC TGCATATAGC AATGCAAAGC CCACTCCACC AGTTAGAACA 
ATGGATAGTC GACTGGCAAC TGCCATGCAT TCCAACTTCA ATGATGCCAT CACTGCTAAT 
AACTACCACT CCTTCCTGAA CACCATGATG CTTCCTTCTT CTCTAGAGGA TGTGCTACCC 
AGACACAACT ATTCTCATTC TTCTCATCTC AAGACAGAAA ATATAGATGA ACAGGGAGTT 
AATTACAGAC AACCTTATTT ATCCAACACC AAGCAAGAGG ACCCAGCTGA TGTGGGAGAT 
TTATACAGAC AGCAATATAT TTCTACTGGA AAGTCTGAGA ATGCTGAGAT AGGAAACACT 
TACCGGCACC CATATCCTTC CAATACTGAG CCAGAAAGTC TAAAGGAGCC ACTAGATCCT 
TTTCTGCATC ACAAGTCAGC AGTTGCTCCA AAGACATATA GAGCAGAAAC ACTTCTACCA 
CATATGCCTC CTCAAGTTTA TGGTTCAAGA TGTGATACTC CATCCTCAAC ATTTTATGGC 
ACCTACATAA ATCAGGCCAA ACACCCCCGC ACCAGGAACA GACCTGATTA TATGTCCTCT 
ATGAGCCCAG GTCTCCGTAG CTACTCAGAA GACACATCTT CCTACCCTAC TATTCGAAGG 
GTACAATCTC TGCATGTGCC TATGCAACCT CCGCCACCTC CTATCCGCAC TGTTCCAATT 
TCAAGAACAG AGGTTCCCCC AGATGATGAT CCAGCATACT GCCCAAGACC AGTTTATCAA 
TACAAACAAT GCCCCCAATT TAACTCTCAG TCAGATTACC ATGTTACCCA GCTGCAGCCT 
TACTTTGAAA ATGGACGAGT TCATTATCGC TACAGTCCCT ATTCAGGGGG CCCAAGTTAT 
TTTTCAGCAG ATAGCAATTT CTATGATATG GACCCCTACA GCACCTTACG TCTGCGACAA 
CTTCCTCTCT ACCCAAGCCG GGACTTTGCT TCTTACACCA CAAGATTGCA GCCCAAAAAC 
ACATATCGGT CACAAGGATT GCCCCCATAT CCCAGAGGAA CTCTACGGGA ACACAATTTT 
ATCAGTAGAG ATGTTCCTCC TGCACTACCA CCTGAACATC CACGACCTCT TCACATTTCT 
TGGGACATGG AAGACATGGA CAGATACAGA CTGCAATCCC TGCGACGAGA GAACAGGGCA 
CGTCAGAGAG CTAAAGGACC AGTAATGTCT CAGTATGATA ATGTTACACC AACCTTAGTG 
GAGGATGTAG CAGGCTTAGA TGTCATACAT CTCCGAAGCC GTTCTGATCC TGGTAAGACT 
CCTGGTCTTT TAAGTGTGGC TGAGAACAAA GACGTCCGTT ACCCAGGAAG GGCAGAAGGT 
GATGAGCATA CAACATATCC ACCTCCTGTT TTTGGAAATG GCCATCAAGA TAAGCCATCT 
CTTCCTCAAA AGCAAAGTGG ATCAAGCCGG GTTCGAATGC AACATGAAAT ATCTACTGAG 
CAGCGGACTC AACCTTCTCA AGATACTTTG CATAGGCAAC CCACAGAGGG ACGAAATGGT 
CCTCCAATTC CTCCTGTGGA CTACAGCTCT AAAGGAATGC CAAATCCCCC AGACCCTGCA 
TCATATCATT CCTCTGCAAA CAAATATAAC TTGACACAAC AGGAACCCAT GCGACTTATT 
CACAAAGAGC TTCGGCTCCC TGAGGATATT GAGCATTCAC ATACCAGGCC AGCTGACAAC 
CAACACCGTG ACTGTTACAG GGAGGAGCCA GCTCAGTTTC CCCTTACTGC ACCTCCTCCT 
AAACCAGAGA GGAGCCATAG CCTTCGAGGT CATAATCCAG ATGTTTTAGA AAGAGACCCT 
AGTATCTTCT ACCCCTACCA AACGCTTCAA GCCAAGAGAC ACAGCACAAT TAATGCAGTG 
TCACAATATG ATAATCTAGC TGATTACCAT TCTATACCCC ATCATCGAAC CACTAACCAG 
TCTACTCCAA ATGCCTTTAA CCTCCCACAT GGACGGACCT ATGCTACTGC CCTGGGCCAG 
GGTGCCTTTC TAGCTGCTGA ACTCGGCTTG CAAAGGCCTG AGACAAAAAT CCATGTGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916227.2
CDS	133..4935
Translation	MKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSDAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_004916170.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916170.4

ORF Insert Sequence:

ATGAAGTCCA GACCCAGCAA ACAGAAACTG AAGCAGAGAG GGATCCTGAG GGAGCGAGTG 
TTTGGTTGTG ACCTTGGGGA ACATCTGCTT AACTCTGGTC AGGATGTCCC CCAAGTCCTC 
AGGAGCTGCA CAGAGTTTAT AGAAAAACAT GGCATTGTTG ATGGAATCTA CCGTCTGTCT 
GGGATTGCAT CTAACATCCA GAAACTACGT CATGAATTTG ACTCTGAACA GATTCCAGAC 
CTGACTAAAG ATGTGTACAT CCAGGACATT CACTGTGTTG GCTCTCTCTG TAAACTCTAC 
TTCCGGGAAC TCCCAAACCC TCTGCTCACA TATCAGTTGT ATGAAAAGTT CTCTGATGCT 
GTGTCAGCGG CTACTGATGA AGAGAGACTG GTGAAAATTC ATGATGTTAT TCAACAGCTT 
CCCCCACCTC ATTACAGAAC ACTGGAGTTC CTTATGCGCC ACTTATCAAG ACTTGCGACA 
TACTGCTCTA TCACCAATAT GCACACTAAA AATTTGGCAA TTGTCTGGGC CCCTAACCTG 
CTCCGGTCAA AACAGATTGA GTCTGCATGT TTTAGCGGTA CAGCAGCTTT CATGGAAGTG 
CGCATACAGT CTGTGGTAGT TGAATTTATT CTAAACCATG TGGAGGTTCT CTTTAGTCCT 
AAACTTAGTT CTGTGATAAG GGATGGTGCT GGTCACACTT CTTTGTCTCG GCCCAAGTCA 
CTGTTGGTGT CATCTCCATC TACAAAACTA CTAAGCCTAG AGGAGGCTCA AGCAAGGACA 
CAGGCCCAAA TCAATTCCCC TGTTGTGGCT GACAGCAAGT ACATTGAGGT GGGAGAGGGA 
CCAGCTGCTT TGCAGGGCAG ATTCCACACT GTAATTGACT TCCCTTCTGA AAGGAAAAGA 
CCGCCAACCA AAATGAAAAA ATCTCCTGTT GGAAGCTGGC GTTCTTTTTT TAGCATAGGC 
AAATCATCAT CTTCAGCCTC CATGTCTAAG CGGAAACTTC AGCGTAATCC AAGTGAGCCT 
TTGGAAATGA AATCCATGGC ATTTGCAGGT GGAAGAGATA GCAGCACCAT GCGCTCAGCT 
AAAAGCGAGG AGTCTCTCAG TTCCTTGCAT GCAGCAGATG GAGAGTCGAA GCTATTTCGT 
CCACGTCGTC CTCGTTCCAG CAGCGATGCT CTGTCTGCTT CATATAATGG GGACCTTCTG 
GATAGCTGCA ACCGTTGCAA CTCCTATGAT AATCTGCCCC GAGACCAAGA CAGTGATGGA 
GATGAGGGTT TGATTCATGT GCCTGCTATG CTGTCAGGTC GCTCTCCTGA GGATGAAGAA 
CTCAGCCCAC CAGACATTGG TATGGCCAGT TTGGACTTTG ACCCAATGTC TTTTCAGTGC 
AGCCCTCCCC AGCTAGAATC TGAAGGACCA GACATTTTTT TTCAGCTTGA CATTTGTGCT 
AGCAATGGCA AGGATAAGGT GCAAAGTGAT GCACAAAACC TAGGCGGTGT CTCCAGCTCC 
GAACCTCACT CTCCTTTCCA AGAGAAAGTC GTTAGTCCAT TTCTATCGCC TGACCGCAGT 
TCTAGTACAG ATAAAGTATC CAAAACTACA TCATTTGCAG AAAAAGTTGT GCAGGCCTTG 
TCACCTAAAG TGGGGCGCAA AGCAGTTCGA TCTCCTCCAT TAACCATCTC AGAGCCTGTC 
ACCATCTCTC TCCCTAGTCG GGTATCGGAA ATGATTGGAA GTATGCAAGG CAATACTGCT 
GCTTCCCAGT CTTTAATTTG GTACAGAGAA AACAGTTTAA ATTCTAGAGA AACTGAAATG 
AATTATGTTT GTAAATCGGT GGATGCCAAC CTTCCATCCT TATTGCCAGC AACAGATCCA 
TGGTCTACAG TTACTCCTGT AATTACTCAA TGGAACCCAG ATGGATTACA GAGAGAAGAC 
AAAGCAGGAC CATTTACTCA TGGCTTTCCC CAGCCAAATA GTGACTTGGA ACAAGTAGAA 
AACTTCAACC TGGAACTTAG CACAACAAAT GTTGCTGCAG CATATCAGAA AGAAGATACA 
GAATTGTCGA GAGCAAATCA GAACAAATCC CACTCTTCTG TTTCTCCCCC TCAAGGAGCT 
TCTGCTTCAG AATCTCCACA GGAGCTAGCC CCTGTCAGTA GTGTTTCTAT CATTCCACCT 
CCACCTCCTC CAAAGAATCC TGCACGCATG CTGGCATTAG CACTGGCTGA ATCTGCTCAA 
CAGGCATCTG CTAATAAAAG ACCTCTTTAT ATGCAGTTCA TGGATCTACC CCCCCATCCT 
CCTCCACCAT CAGTACTAGA GGACAAACCA CTTCTTGAGT TATCTCCAAA AATGTCTCCA 
GAGCACCTTG TATCTGGTCC TGCAACACCA GCGACCAGTC CTCCAGTCAT ATCCAATAAA 
ACATCTCCAG CTACTCCACC AAGCACAACT TCATCATTTT CTATCACAAC AATTCATCAC 
AGTCCTGTGA AAAACCATAG TCCTCTGGCA GGACAGATGC CTACAGCAGT CACTCCTGGA 
CTCAGTCCTT CATCAAACCA GGTTCCTGTA TCCTTTCTAT CTGATGAAAT GCTACCTTCT 
GAAACAAATA AAATGAATTC TGTCATACTG AGTGAAGCAT TTACAACAGG AATTCCTGTT 
ACTCCACCAT CTGAAAAACC CAAGGAAATT TCTAGACCAC CACATCTTCA CCAGCGTTCT 
GAATCCTTTC CTTCACATCC TGCATATAGC AATGCAAAGC CCACTCCACC AGTTAGAACA 
ATGGATAGTC GACTGGCAAC TGCCATGCAT TCCAACTTCA ATGATGCCAT CACTGCTAAT 
AACTACCACT CCTTCCTGAA CACCATGATG CTTCCTTCTT CTCTAGAGGA TGTGCTACCC 
AGACACAACT ATTCTCATTC TTCTCATCTC AAGACAGAAA ATATAGATGA ACAGGGAGTT 
AATTACAGAC AACCTTATTT ATCCAACACC AAGCAAGAGG ACCCAGCTGA TGTGGGAGAT 
TTATACAGAC AGCAATATAT TTCTACTGGA AAGTCTGAGA ATGCTGAGAT AGGAAACACT 
TACCGGCACC CATATCCTTC CAATACTGAG CCAGAAAGTC TAAAGGAGCC ACTAGATCCT 
TTTCTGCATC ACAAGTCAGC AGTTGCTCCA AAGACATATA GAGCAGAAAC ACTTCTACCA 
CATATGCCTC CTCAAGTTTA TGGTTCAAGA TGTGATACTC CATCCTCAAC ATTTTATGGC 
ACCTACATAA ATCAGGCCAA ACACCCCCGC ACCAGGAACA GACCTGATTA TATGTCCTCT 
ATGAGCCCAG GTCTCCGTAG CTACTCAGAA GACACATCTT CCTACCCTAC TATTCGAAGG 
GTACAATCTC TGCATGTGCC TATGCAACCT CCGCCACCTC CTATCCGCAC TGTTCCAATT 
TCAAGAACAG AGGTTCCCCC AGATGATGAT CCAGCATACT GCCCAAGACC AGTTTATCAA 
TACAAACAAT GCCCCCAATT TAACTCTCAG TCAGATTACC ATGTTACCCA GCTGCAGCCT 
TACTTTGAAA ATGGACGAGT TCATTATCGC TACAGTCCCT ATTCAGGGGG CCCAAGTTAT 
TTTTCAGCAG ATAGCAATTT CTATGATATG GACCCCTACA GCACCTTACG TCTGCGACAA 
CTTCCTCTCT ACCCAAGCCG GGACTTTGCT TCTTACACCA CAAGATTGCA GCCCAAAAAC 
ACATATCGGT CACAAGGATT GCCCCCATAT CCCAGAGGAA CTCTACGGGA ACACAATTTT 
ATCAGTAGAG ATGTTCCTCC TGCACTACCA CCTGAACATC CACGACCTCT TCACATTTCT 
TGGGACATGG AAGACATGGA CAGATACAGA CTGCAATCCC TGCGACGAGA GAACAGGGCA 
CGTCAGAGAG CTAAAGGACC AGTAATGTCT CAGTATGATA ATGTTACACC AACCTTAGTG 
GAGGATGTAG CAGGCTTAGA TGTCATACAT CTCCGAAGCC GTTCTGATCC TGGTAAGACT 
CCTGGTCTTT TAAGTGTGGC TGAGAACAAA GACGTCCGTT ACCCAGGAAG GGCAGAAGGT 
GATGAGCATA CAACATATCC ACCTCCTGTT TTTGGAAATG GCCATCAAGA TAAGCCATCT 
CTTCCTCAAA AGCAAAGTGG ATCAAGCCGG GTTCGAATGC AACATGAAAT ATCTACTGAG 
CAGCGGACTC AACCTTCTCA AGATACTTTG CATAGGCAAC CCACAGAGGG ACGAAATGGT 
CCTCCAATTC CTCCTGTGGA CTACAGCTCT AAAGGAATGC CAAATCCCCC AGACCCTGCA 
TCATATCATT CCTCTGCAAA CAAATATAAC TTGACACAAC AGGAACCCAT GCGACTTATT 
CACAAAGAGC TTCGGCTCCC TGAGGATATT GAGCATTCAC ATACCAGGCC AGCTGACAAC 
CAACACCGTG ACTGTTACAG GGAGGAGCCA GCTCAGTTTC CCCTTACTGC ACCTCCTCCT 
AAACCAGAGA GGAGCCATAG CCTTCGAGGT CATAATCCAG ATGTTTTAGA AAGAGACCCT 
AGTATCTTCT ACCCCTACCA AACGCTTCAA GCCAAGAGAC ACAGCACAAT TAATGCAGTG 
TCACAATATG ATAATCTAGC TGATTACCAT TCTATACCCC ATCATCGAAC CACTAACCAG 
TCTACTCCAA ATGCCTTTAA CCTCCCACAT GGACGGACCT ATGCTACTGC CCTGGGCCAG 
GGTGCCTTTC TAGCTGCTGA ACTCGGCTTG CAAAGGCCTG AGACAAAAAT CCATGTGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_004916169.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916226.2 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X11, mRNA.

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CloneID	OXa61968
Clone ID Related Accession (Same CDS sequence)	XM_004916170.4 , XM_004916169.4
Accession Version	XM_004916169.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916169.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCCA GACCCAGCAA ACAGAAACTG AAGCAGAGAG GGATCCTGAG GGAGCGAGTG 
TTTGGTTGTG ACCTTGGGGA ACATCTGCTT AACTCTGGTC AGGATGTCCC CCAAGTCCTC 
AGGAGCTGCA CAGAGTTTAT AGAAAAACAT GGCATTGTTG ATGGAATCTA CCGTCTGTCT 
GGGATTGCAT CTAACATCCA GAAACTACGT CATGAATTTG ACTCTGAACA GATTCCAGAC 
CTGACTAAAG ATGTGTACAT CCAGGACATT CACTGTGTTG GCTCTCTCTG TAAACTCTAC 
TTCCGGGAAC TCCCAAACCC TCTGCTCACA TATCAGTTGT ATGAAAAGTT CTCTGATGCT 
GTGTCAGCGG CTACTGATGA AGAGAGACTG GTGAAAATTC ATGATGTTAT TCAACAGCTT 
CCCCCACCTC ATTACAGAAC ACTGGAGTTC CTTATGCGCC ACTTATCAAG ACTTGCGACA 
TACTGCTCTA TCACCAATAT GCACACTAAA AATTTGGCAA TTGTCTGGGC CCCTAACCTG 
CTCCGGTCAA AACAGATTGA GTCTGCATGT TTTAGCGGTA CAGCAGCTTT CATGGAAGTG 
CGCATACAGT CTGTGGTAGT TGAATTTATT CTAAACCATG TGGAGGTTCT CTTTAGTCCT 
AAACTTAGTT CTGTGATAAG GGATGGTGCT GGTCACACTT CTTTGTCTCG GCCCAAGTCA 
CTGTTGGTGT CATCTCCATC TACAAAACTA CTAAGCCTAG AGGAGGCTCA AGCAAGGACA 
CAGGCCCAAA TCAATTCCCC TGTTGTGGCT GACAGCAAGT ACATTGAGGT GGGAGAGGGA 
CCAGCTGCTT TGCAGGGCAG ATTCCACACT GTAATTGACT TCCCTTCTGA AAGGAAAAGA 
CCGCCAACCA AAATGAAAAA ATCTCCTGTT GGAAGCTGGC GTTCTTTTTT TAGCATAGGC 
AAATCATCAT CTTCAGCCTC CATGTCTAAG CGGAAACTTC AGCGTAATCC AAGTGAGCCT 
TTGGAAATGA AATCCATGGC ATTTGCAGGT GGAAGAGATA GCAGCACCAT GCGCTCAGCT 
AAAAGCGAGG AGTCTCTCAG TTCCTTGCAT GCAGCAGATG GAGAGTCGAA GCTATTTCGT 
CCACGTCGTC CTCGTTCCAG CAGCGATGCT CTGTCTGCTT CATATAATGG GGACCTTCTG 
GATAGCTGCA ACCGTTGCAA CTCCTATGAT AATCTGCCCC GAGACCAAGA CAGTGATGGA 
GATGAGGGTT TGATTCATGT GCCTGCTATG CTGTCAGGTC GCTCTCCTGA GGATGAAGAA 
CTCAGCCCAC CAGACATTGG TATGGCCAGT TTGGACTTTG ACCCAATGTC TTTTCAGTGC 
AGCCCTCCCC AGCTAGAATC TGAAGGACCA GACATTTTTT TTCAGCTTGA CATTTGTGCT 
AGCAATGGCA AGGATAAGGT GCAAAGTGAT GCACAAAACC TAGGCGGTGT CTCCAGCTCC 
GAACCTCACT CTCCTTTCCA AGAGAAAGTC GTTAGTCCAT TTCTATCGCC TGACCGCAGT 
TCTAGTACAG ATAAAGTATC CAAAACTACA TCATTTGCAG AAAAAGTTGT GCAGGCCTTG 
TCACCTAAAG TGGGGCGCAA AGCAGTTCGA TCTCCTCCAT TAACCATCTC AGAGCCTGTC 
ACCATCTCTC TCCCTAGTCG GGTATCGGAA ATGATTGGAA GTATGCAAGG CAATACTGCT 
GCTTCCCAGT CTTTAATTTG GTACAGAGAA AACAGTTTAA ATTCTAGAGA AACTGAAATG 
AATTATGTTT GTAAATCGGT GGATGCCAAC CTTCCATCCT TATTGCCAGC AACAGATCCA 
TGGTCTACAG TTACTCCTGT AATTACTCAA TGGAACCCAG ATGGATTACA GAGAGAAGAC 
AAAGCAGGAC CATTTACTCA TGGCTTTCCC CAGCCAAATA GTGACTTGGA ACAAGTAGAA 
AACTTCAACC TGGAACTTAG CACAACAAAT GTTGCTGCAG CATATCAGAA AGAAGATACA 
GAATTGTCGA GAGCAAATCA GAACAAATCC CACTCTTCTG TTTCTCCCCC TCAAGGAGCT 
TCTGCTTCAG AATCTCCACA GGAGCTAGCC CCTGTCAGTA GTGTTTCTAT CATTCCACCT 
CCACCTCCTC CAAAGAATCC TGCACGCATG CTGGCATTAG CACTGGCTGA ATCTGCTCAA 
CAGGCATCTG CTAATAAAAG ACCTCTTTAT ATGCAGTTCA TGGATCTACC CCCCCATCCT 
CCTCCACCAT CAGTACTAGA GGACAAACCA CTTCTTGAGT TATCTCCAAA AATGTCTCCA 
GAGCACCTTG TATCTGGTCC TGCAACACCA GCGACCAGTC CTCCAGTCAT ATCCAATAAA 
ACATCTCCAG CTACTCCACC AAGCACAACT TCATCATTTT CTATCACAAC AATTCATCAC 
AGTCCTGTGA AAAACCATAG TCCTCTGGCA GGACAGATGC CTACAGCAGT CACTCCTGGA 
CTCAGTCCTT CATCAAACCA GGTTCCTGTA TCCTTTCTAT CTGATGAAAT GCTACCTTCT 
GAAACAAATA AAATGAATTC TGTCATACTG AGTGAAGCAT TTACAACAGG AATTCCTGTT 
ACTCCACCAT CTGAAAAACC CAAGGAAATT TCTAGACCAC CACATCTTCA CCAGCGTTCT 
GAATCCTTTC CTTCACATCC TGCATATAGC AATGCAAAGC CCACTCCACC AGTTAGAACA 
ATGGATAGTC GACTGGCAAC TGCCATGCAT TCCAACTTCA ATGATGCCAT CACTGCTAAT 
AACTACCACT CCTTCCTGAA CACCATGATG CTTCCTTCTT CTCTAGAGGA TGTGCTACCC 
AGACACAACT ATTCTCATTC TTCTCATCTC AAGACAGAAA ATATAGATGA ACAGGGAGTT 
AATTACAGAC AACCTTATTT ATCCAACACC AAGCAAGAGG ACCCAGCTGA TGTGGGAGAT 
TTATACAGAC AGCAATATAT TTCTACTGGA AAGTCTGAGA ATGCTGAGAT AGGAAACACT 
TACCGGCACC CATATCCTTC CAATACTGAG CCAGAAAGTC TAAAGGAGCC ACTAGATCCT 
TTTCTGCATC ACAAGTCAGC AGTTGCTCCA AAGACATATA GAGCAGAAAC ACTTCTACCA 
CATATGCCTC CTCAAGTTTA TGGTTCAAGA TGTGATACTC CATCCTCAAC ATTTTATGGC 
ACCTACATAA ATCAGGCCAA ACACCCCCGC ACCAGGAACA GACCTGATTA TATGTCCTCT 
ATGAGCCCAG GTCTCCGTAG CTACTCAGAA GACACATCTT CCTACCCTAC TATTCGAAGG 
GTACAATCTC TGCATGTGCC TATGCAACCT CCGCCACCTC CTATCCGCAC TGTTCCAATT 
TCAAGAACAG AGGTTCCCCC AGATGATGAT CCAGCATACT GCCCAAGACC AGTTTATCAA 
TACAAACAAT GCCCCCAATT TAACTCTCAG TCAGATTACC ATGTTACCCA GCTGCAGCCT 
TACTTTGAAA ATGGACGAGT TCATTATCGC TACAGTCCCT ATTCAGGGGG CCCAAGTTAT 
TTTTCAGCAG ATAGCAATTT CTATGATATG GACCCCTACA GCACCTTACG TCTGCGACAA 
CTTCCTCTCT ACCCAAGCCG GGACTTTGCT TCTTACACCA CAAGATTGCA GCCCAAAAAC 
ACATATCGGT CACAAGGATT GCCCCCATAT CCCAGAGGAA CTCTACGGGA ACACAATTTT 
ATCAGTAGAG ATGTTCCTCC TGCACTACCA CCTGAACATC CACGACCTCT TCACATTTCT 
TGGGACATGG AAGACATGGA CAGATACAGA CTGCAATCCC TGCGACGAGA GAACAGGGCA 
CGTCAGAGAG CTAAAGGACC AGTAATGTCT CAGTATGATA ATGTTACACC AACCTTAGTG 
GAGGATGTAG CAGGCTTAGA TGTCATACAT CTCCGAAGCC GTTCTGATCC TGGTAAGACT 
CCTGGTCTTT TAAGTGTGGC TGAGAACAAA GACGTCCGTT ACCCAGGAAG GGCAGAAGGT 
GATGAGCATA CAACATATCC ACCTCCTGTT TTTGGAAATG GCCATCAAGA TAAGCCATCT 
CTTCCTCAAA AGCAAAGTGG ATCAAGCCGG GTTCGAATGC AACATGAAAT ATCTACTGAG 
CAGCGGACTC AACCTTCTCA AGATACTTTG CATAGGCAAC CCACAGAGGG ACGAAATGGT 
CCTCCAATTC CTCCTGTGGA CTACAGCTCT AAAGGAATGC CAAATCCCCC AGACCCTGCA 
TCATATCATT CCTCTGCAAA CAAATATAAC TTGACACAAC AGGAACCCAT GCGACTTATT 
CACAAAGAGC TTCGGCTCCC TGAGGATATT GAGCATTCAC ATACCAGGCC AGCTGACAAC 
CAACACCGTG ACTGTTACAG GGAGGAGCCA GCTCAGTTTC CCCTTACTGC ACCTCCTCCT 
AAACCAGAGA GGAGCCATAG CCTTCGAGGT CATAATCCAG ATGTTTTAGA AAGAGACCCT 
AGTATCTTCT ACCCCTACCA AACGCTTCAA GCCAAGAGAC ACAGCACAAT TAATGCAGTG 
TCACAATATG ATAATCTAGC TGATTACCAT TCTATACCCC ATCATCGAAC CACTAACCAG 
TCTACTCCAA ATGCCTTTAA CCTCCCACAT GGACGGACCT ATGCTACTGC CCTGGGCCAG 
GGTGCCTTTC TAGCTGCTGA ACTCGGCTTG CAAAGGCCTG AGACAAAAAT CCATGTGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916226.2
CDS	321..5123
Translation	MKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSDAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_004916169.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916169.4

ORF Insert Sequence:

ATGAAGTCCA GACCCAGCAA ACAGAAACTG AAGCAGAGAG GGATCCTGAG GGAGCGAGTG 
TTTGGTTGTG ACCTTGGGGA ACATCTGCTT AACTCTGGTC AGGATGTCCC CCAAGTCCTC 
AGGAGCTGCA CAGAGTTTAT AGAAAAACAT GGCATTGTTG ATGGAATCTA CCGTCTGTCT 
GGGATTGCAT CTAACATCCA GAAACTACGT CATGAATTTG ACTCTGAACA GATTCCAGAC 
CTGACTAAAG ATGTGTACAT CCAGGACATT CACTGTGTTG GCTCTCTCTG TAAACTCTAC 
TTCCGGGAAC TCCCAAACCC TCTGCTCACA TATCAGTTGT ATGAAAAGTT CTCTGATGCT 
GTGTCAGCGG CTACTGATGA AGAGAGACTG GTGAAAATTC ATGATGTTAT TCAACAGCTT 
CCCCCACCTC ATTACAGAAC ACTGGAGTTC CTTATGCGCC ACTTATCAAG ACTTGCGACA 
TACTGCTCTA TCACCAATAT GCACACTAAA AATTTGGCAA TTGTCTGGGC CCCTAACCTG 
CTCCGGTCAA AACAGATTGA GTCTGCATGT TTTAGCGGTA CAGCAGCTTT CATGGAAGTG 
CGCATACAGT CTGTGGTAGT TGAATTTATT CTAAACCATG TGGAGGTTCT CTTTAGTCCT 
AAACTTAGTT CTGTGATAAG GGATGGTGCT GGTCACACTT CTTTGTCTCG GCCCAAGTCA 
CTGTTGGTGT CATCTCCATC TACAAAACTA CTAAGCCTAG AGGAGGCTCA AGCAAGGACA 
CAGGCCCAAA TCAATTCCCC TGTTGTGGCT GACAGCAAGT ACATTGAGGT GGGAGAGGGA 
CCAGCTGCTT TGCAGGGCAG ATTCCACACT GTAATTGACT TCCCTTCTGA AAGGAAAAGA 
CCGCCAACCA AAATGAAAAA ATCTCCTGTT GGAAGCTGGC GTTCTTTTTT TAGCATAGGC 
AAATCATCAT CTTCAGCCTC CATGTCTAAG CGGAAACTTC AGCGTAATCC AAGTGAGCCT 
TTGGAAATGA AATCCATGGC ATTTGCAGGT GGAAGAGATA GCAGCACCAT GCGCTCAGCT 
AAAAGCGAGG AGTCTCTCAG TTCCTTGCAT GCAGCAGATG GAGAGTCGAA GCTATTTCGT 
CCACGTCGTC CTCGTTCCAG CAGCGATGCT CTGTCTGCTT CATATAATGG GGACCTTCTG 
GATAGCTGCA ACCGTTGCAA CTCCTATGAT AATCTGCCCC GAGACCAAGA CAGTGATGGA 
GATGAGGGTT TGATTCATGT GCCTGCTATG CTGTCAGGTC GCTCTCCTGA GGATGAAGAA 
CTCAGCCCAC CAGACATTGG TATGGCCAGT TTGGACTTTG ACCCAATGTC TTTTCAGTGC 
AGCCCTCCCC AGCTAGAATC TGAAGGACCA GACATTTTTT TTCAGCTTGA CATTTGTGCT 
AGCAATGGCA AGGATAAGGT GCAAAGTGAT GCACAAAACC TAGGCGGTGT CTCCAGCTCC 
GAACCTCACT CTCCTTTCCA AGAGAAAGTC GTTAGTCCAT TTCTATCGCC TGACCGCAGT 
TCTAGTACAG ATAAAGTATC CAAAACTACA TCATTTGCAG AAAAAGTTGT GCAGGCCTTG 
TCACCTAAAG TGGGGCGCAA AGCAGTTCGA TCTCCTCCAT TAACCATCTC AGAGCCTGTC 
ACCATCTCTC TCCCTAGTCG GGTATCGGAA ATGATTGGAA GTATGCAAGG CAATACTGCT 
GCTTCCCAGT CTTTAATTTG GTACAGAGAA AACAGTTTAA ATTCTAGAGA AACTGAAATG 
AATTATGTTT GTAAATCGGT GGATGCCAAC CTTCCATCCT TATTGCCAGC AACAGATCCA 
TGGTCTACAG TTACTCCTGT AATTACTCAA TGGAACCCAG ATGGATTACA GAGAGAAGAC 
AAAGCAGGAC CATTTACTCA TGGCTTTCCC CAGCCAAATA GTGACTTGGA ACAAGTAGAA 
AACTTCAACC TGGAACTTAG CACAACAAAT GTTGCTGCAG CATATCAGAA AGAAGATACA 
GAATTGTCGA GAGCAAATCA GAACAAATCC CACTCTTCTG TTTCTCCCCC TCAAGGAGCT 
TCTGCTTCAG AATCTCCACA GGAGCTAGCC CCTGTCAGTA GTGTTTCTAT CATTCCACCT 
CCACCTCCTC CAAAGAATCC TGCACGCATG CTGGCATTAG CACTGGCTGA ATCTGCTCAA 
CAGGCATCTG CTAATAAAAG ACCTCTTTAT ATGCAGTTCA TGGATCTACC CCCCCATCCT 
CCTCCACCAT CAGTACTAGA GGACAAACCA CTTCTTGAGT TATCTCCAAA AATGTCTCCA 
GAGCACCTTG TATCTGGTCC TGCAACACCA GCGACCAGTC CTCCAGTCAT ATCCAATAAA 
ACATCTCCAG CTACTCCACC AAGCACAACT TCATCATTTT CTATCACAAC AATTCATCAC 
AGTCCTGTGA AAAACCATAG TCCTCTGGCA GGACAGATGC CTACAGCAGT CACTCCTGGA 
CTCAGTCCTT CATCAAACCA GGTTCCTGTA TCCTTTCTAT CTGATGAAAT GCTACCTTCT 
GAAACAAATA AAATGAATTC TGTCATACTG AGTGAAGCAT TTACAACAGG AATTCCTGTT 
ACTCCACCAT CTGAAAAACC CAAGGAAATT TCTAGACCAC CACATCTTCA CCAGCGTTCT 
GAATCCTTTC CTTCACATCC TGCATATAGC AATGCAAAGC CCACTCCACC AGTTAGAACA 
ATGGATAGTC GACTGGCAAC TGCCATGCAT TCCAACTTCA ATGATGCCAT CACTGCTAAT 
AACTACCACT CCTTCCTGAA CACCATGATG CTTCCTTCTT CTCTAGAGGA TGTGCTACCC 
AGACACAACT ATTCTCATTC TTCTCATCTC AAGACAGAAA ATATAGATGA ACAGGGAGTT 
AATTACAGAC AACCTTATTT ATCCAACACC AAGCAAGAGG ACCCAGCTGA TGTGGGAGAT 
TTATACAGAC AGCAATATAT TTCTACTGGA AAGTCTGAGA ATGCTGAGAT AGGAAACACT 
TACCGGCACC CATATCCTTC CAATACTGAG CCAGAAAGTC TAAAGGAGCC ACTAGATCCT 
TTTCTGCATC ACAAGTCAGC AGTTGCTCCA AAGACATATA GAGCAGAAAC ACTTCTACCA 
CATATGCCTC CTCAAGTTTA TGGTTCAAGA TGTGATACTC CATCCTCAAC ATTTTATGGC 
ACCTACATAA ATCAGGCCAA ACACCCCCGC ACCAGGAACA GACCTGATTA TATGTCCTCT 
ATGAGCCCAG GTCTCCGTAG CTACTCAGAA GACACATCTT CCTACCCTAC TATTCGAAGG 
GTACAATCTC TGCATGTGCC TATGCAACCT CCGCCACCTC CTATCCGCAC TGTTCCAATT 
TCAAGAACAG AGGTTCCCCC AGATGATGAT CCAGCATACT GCCCAAGACC AGTTTATCAA 
TACAAACAAT GCCCCCAATT TAACTCTCAG TCAGATTACC ATGTTACCCA GCTGCAGCCT 
TACTTTGAAA ATGGACGAGT TCATTATCGC TACAGTCCCT ATTCAGGGGG CCCAAGTTAT 
TTTTCAGCAG ATAGCAATTT CTATGATATG GACCCCTACA GCACCTTACG TCTGCGACAA 
CTTCCTCTCT ACCCAAGCCG GGACTTTGCT TCTTACACCA CAAGATTGCA GCCCAAAAAC 
ACATATCGGT CACAAGGATT GCCCCCATAT CCCAGAGGAA CTCTACGGGA ACACAATTTT 
ATCAGTAGAG ATGTTCCTCC TGCACTACCA CCTGAACATC CACGACCTCT TCACATTTCT 
TGGGACATGG AAGACATGGA CAGATACAGA CTGCAATCCC TGCGACGAGA GAACAGGGCA 
CGTCAGAGAG CTAAAGGACC AGTAATGTCT CAGTATGATA ATGTTACACC AACCTTAGTG 
GAGGATGTAG CAGGCTTAGA TGTCATACAT CTCCGAAGCC GTTCTGATCC TGGTAAGACT 
CCTGGTCTTT TAAGTGTGGC TGAGAACAAA GACGTCCGTT ACCCAGGAAG GGCAGAAGGT 
GATGAGCATA CAACATATCC ACCTCCTGTT TTTGGAAATG GCCATCAAGA TAAGCCATCT 
CTTCCTCAAA AGCAAAGTGG ATCAAGCCGG GTTCGAATGC AACATGAAAT ATCTACTGAG 
CAGCGGACTC AACCTTCTCA AGATACTTTG CATAGGCAAC CCACAGAGGG ACGAAATGGT 
CCTCCAATTC CTCCTGTGGA CTACAGCTCT AAAGGAATGC CAAATCCCCC AGACCCTGCA 
TCATATCATT CCTCTGCAAA CAAATATAAC TTGACACAAC AGGAACCCAT GCGACTTATT 
CACAAAGAGC TTCGGCTCCC TGAGGATATT GAGCATTCAC ATACCAGGCC AGCTGACAAC 
CAACACCGTG ACTGTTACAG GGAGGAGCCA GCTCAGTTTC CCCTTACTGC ACCTCCTCCT 
AAACCAGAGA GGAGCCATAG CCTTCGAGGT CATAATCCAG ATGTTTTAGA AAGAGACCCT 
AGTATCTTCT ACCCCTACCA AACGCTTCAA GCCAAGAGAC ACAGCACAAT TAATGCAGTG 
TCACAATATG ATAATCTAGC TGATTACCAT TCTATACCCC ATCATCGAAC CACTAACCAG 
TCTACTCCAA ATGCCTTTAA CCTCCCACAT GGACGGACCT ATGCTACTGC CCTGGGCCAG 
GGTGCCTTTC TAGCTGCTGA ACTCGGCTTG CAAAGGCCTG AGACAAAAAT CCATGTGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_004916165.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916222.1 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X2, mRNA.

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CloneID	OXa13195
Clone ID Related Accession (Same CDS sequence)	XM_004916165.3 , XM_004916164.3
Accession Version	XM_004916165.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916165.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGTCTCC 
CCTGCGCATG GAATGAGGCC GGCCTCCTGG AGCTACTTTC CCCCCTATTT GGAGATGGAT 
AGTTTAAGGC CTGGAACATC GTGCTGTTCT GAGGTATTAA GTCACTCGCA GCTAAGCTCA 
GTGTCCCGGA AGCACGGCAA GCTCATCACA TTTCTAAGAA CCTTCATGAA GTCCAGACCC 
AGCAAACAGA AACTGAAGCA GAGAGGGATC CTGAGGGAGC GAGTGTTTGG TTGTGACCTT 
GGGGAACATC TGCTTAACTC TGGTCAGGAT GTCCCCCAAG TCCTCAGGAG CTGCACAGAG 
TTTATAGAAA AACATGGCAT TGTTGATGGA ATCTACCGTC TGTCTGGGAT TGCATCTAAC 
ATCCAGAAAC TACGTCATGA ATTTGACTCT GAACAGATTC CAGACCTGAC TAAAGATGTG 
TACATCCAGG ACATTCACTG TGTTGGCTCT CTCTGTAAAC TCTACTTCCG GGAACTCCCA 
AACCCTCTGC TCACATATCA GTTGTATGAA AAGTTCTCTG ATGCTGTGTC AGCGGCTACT 
GATGAAGAGA GACTGGTGAA AATTCATGAT GTTATTCAAC AGCTTCCCCC ACCTCATTAC 
AGAACACTGG AGTTCCTTAT GCGCCACTTA TCAAGACTTG CGACATACTG CTCTATCACC 
AATATGCACA CTAAAAATTT GGCAATTGTC TGGGCCCCTA ACCTGCTCCG GTCAAAACAG 
ATTGAGTCTG CATGTTTTAG CGGTACAGCA GCTTTCATGG AAGTGCGCAT ACAGTCTGTG 
GTAGTTGAAT TTATTCTAAA CCATGTGGAG GTTCTCTTTA GTCCTAAACT TAGTTCTGTG 
ATAAGGGATG GTGCTGGTCA CACTTCTTTG TCTCGGCCCA AGTCACTGTT GGTGTCATCT 
CCATCTACAA AACTACTAAG CCTAGAGGAG GCTCAAGCAA GGACACAGGC CCAAATCAAT 
TCCCCTGTTG TGGCTGACAG CAAGTACATT GAGGTGGGAG AGGGACCAGC TGCTTTGCAG 
GGCAGATTCC ACACTGTAAT TGACTTCCCT TCTGAAAGGA AAAGACCGCC AACCAAAATG 
AAAAAATCTC CTGTTGGAAG CTGGCGTTCT TTTTTTAGCA TAGGCAAATC ATCATCTTCA 
GCCTCCATGT CTAAGCGGAA ACTTCAGCGT AATCCAAGTG AGCCTTTGGA AATGAAATCC 
ATGGCATTTG CAGGTGGAAG AGATAGCAGC ACCATGCGCT CAGCTAAAAG CGAGGAGTCT 
CTCAGTTCCT TGCATGCAGC AGATGGAGAG TCGAAGCTAT TTCGTCCACG TCGTCCTCGT 
TCCAGCAGCG ATGCTCTGTC TGCTTCATAT AATGGGGACC TTCTGGATAG CTGCAACCGT 
TGCAACTCCT ATGATAATCT GCCCCGAGAC CAAGACAGTG ATGGAGATGA GGGTTTGATT 
CATGTGCCTG CTATGCTGTC AGGTCGCTCT CCTGAGGATG AAGAACTCAG CCCACCAGAC 
ATTGGTATGG CCAGTTTGGA CTTTGACCCA ATGTCTTTTC AGTGCAGCCC TCCCCAGCTA 
GAATCTGAAG GACCAGACAT TTTTTTTCAG CTTGACATTT GTGCTAGCAA TGGCAAGGAT 
AAGGTGCAAA GTGGTGCACA AAACCTAGGC GGTGTCTCCA GCTCCGAACC TCACTCTCCT 
TTCCAAGAGA AAGTCGTTAG TCCATTTCTA TCGCCTGACC GCAGTTCTAG TACAGATAAA 
GTATCCAAAA CTACATCATT TGCAGAAAAA GTTGTGCAGG CCTTGTCACC TAAAGTGGGG 
CGCAAAGCAG TTCGATCTCC TCCATTAACC ATCTCAGAGC CTGTCACCAT CTCTCTCCCT 
AGTCGGGTAT CGGAAATGAT TGGAAGTATG CAAGGCAATA CTGCTGCTTC CCAGTCTTTA 
ATTTGGTACA GAGAAAACAG TTTAAATTCT AGAGAAACTG AAATGAATTA TGTTTGTAAA 
TCGGTGGATG CCAACCTTCC ATCCTTATTG CCAGCAACAG ATCCATGGTC TACAGTTACT 
CCTGTAATTA CTCAATGGAA CCCAGATGGA TTACAGAGAG AAGACAAAGC AGGACCATTT 
ACTCATGGCT TTCCCCAGCC AAATAGTGAC TTGGAACAAG TAGAAAACTT CAACCTGGAA 
CTTAGCACAA CAAATGTTGC TGCAGCATAT CAGAAAGAAG ATACAGAATT GTCGAGAGCA 
AATCAGAACA AATCCCACTC TTCTGTTTCT CCCCCTCAAG GAGCTTCTGC TTCAGAATCT 
CCACAGGAGC TAGCCCCTGT CAGTAGTGTT TCTATCATTC CACCTCCACC TCCTCCAAAG 
AATCCTGCAC GCATGCTGGC ATTAGCACTG GCTGAATCTG CTCAACAGGC ATCTGCTAAT 
AAAAGACCTC TTTATATGCA GTTCATGGAT CTACCCCCCC ATCCTCCTCC ACCATCAGTA 
CTAGAGGACA AACCACTTCT TGAGTTATCT CCAAAAATGT CTCCAGAGCA CCTCGTATCT 
GGTCCTGCAA CACCAGCGAC CAGTCCTCCA GTCATATCCA ATAAAACATC TCCAGCTACT 
CCACCAAGCA CAACTTCATC ATTTTCTATC ACAACAATTC ATCACAGTCC TGTGAAAAAC 
CATAGTCCTC TGGCAGGACA GATGCCTACA GCAGTCACTC CTGGACTCAG TCCTTCATCA 
AACCAGGTTC CTGTATCCTT TCTATCTGAT GAAATGCTAC CTTCTGAAAC AAATAAAATG 
AATTCTGTCA TACTGAGTGA AGCATTTACA ACAGGAATTC CTGTTACTCC ACCATCTGAA 
AAACCCAAGG AAATTTCTAG ACCACCACAT CTTCACCAGC GTTCTGAATC CTTTCCTTCA 
CATCCTGCAT ATAGCAATGC AAAGCCCACT CCACCAGTTA GAACAATGGA TAGTCGACTG 
GCAACTGCCA TGCATTCCAA CTTCAATGAT GCCATCACTG CTAATAACTA CCACTCCTTC 
CTGAACACCA TGATGCTTCC TTCTTCTCTA GAGGATGTGC TACCCAGACA CAACTATTCT 
CATTCTTCTC ATCTCAAGAC AGAAAATATA GATGAACAGG GAGTTAATTA CAGACAACCT 
TATTTATCCA ACACCAAGCA AGAGGACCCA GCTGATGTGG GAGATTTATA CAGACAGCAA 
TATATTTCTA CTGGAAAGTC TGAGAATGCT GAGATAGGAA ACACTTACCG GCACCCATAT 
CCTTCCAATA CTGAGCCAGA AAGTCTAAAG GAGCCACTAG ATCCTTTTCT GCATCACAAG 
TCAGCAGTTG CTCCAAAGAC ATATAGAGCA GAAACACTTC TACCACATAT GCCTCCTCAA 
GTTTATGGTT CAAGATGTGA TACTCCATCC TCAACATTTT ATGGCACCTA CATAAATCAG 
GCCAAACACC CCCGCACCAG GAACAGACCT GATTATATGT CCTCTATGAG CCCAGGTCTC 
CGTAGCTACT CAGAAGACAC ATCTTCCTAC CCTACTATTC GAAGGGTACA ATCTCTGCAT 
GTGCCTATGC AACCTCCGCC ACCTCCTATC CGCACTGTTC CAATTTCAAG AACAGAGGTT 
CCCCCAGATG ATGATCCAGC ATACTGCCCA AGACCAGTTT ATCAATACAA ACAATGCCCC 
CAATTTAACT CTCAGTCAGA TTACCATGTT ACCCAGCTGC AGCCTTACTT TGAAAATGGA 
CGAGTTCATT ATCGCTACAG TCCCTATTCA GGGGGCCCAA GTTATTTTTC AGCAGATAGC 
AATTTCTATG ATATGGACCC CTACAGCACC TTACGTCTGC GACAACTTCC TCTCTACCCA 
AGCCGGGACT TTGCTTCTTA CACCACAAGA TTGCAGCCCA AAAACACATA TCGGTCACAA 
GGATTGCCCC CATATCCCAG AGGAACTCTA CGGGAACACA ATTTTATCAG TAGAGATGTT 
CCTCCTGCAC TACCACCTGA ACATCCACGA CCTCTTCACA TTTCTTGGGA CATGGAAGAC 
ATGGACAGAT ACAGACTGCA ATCCCTGCGA CGAGAGAACA GGGCACGTCA GAGAGCTAAA 
GGACCAGTAA TGTCTCAGTA TGATAATGTT ACACCAACCT TAGTGGAGGA TGTAGCAGGC 
TTAGATGTCA TACATCTCCG AAGCCGTTCT GATCCTGGTA AGACTCCTGG TCTTTTAAGT 
GTGGCTGAGA ACAAAGACGT CCGTTACCCA GGAAGGGCAG AAGGTGATGA GCATACAACA 
TATCCACCTC CTGTTTTTGG AAATGGCCAT CAAGATAAGC CATCTCTTCC TCAAAAGCAA 
AGTGGATCAA GCCGGGTTCG AATGCAACAT GAAATATCTA CTGAGCAGCG GACTCAACCT 
TCTCAAGATA CTTTGCATAG GCAACCCACA GAGGGACGAA ATGGTCCTCC AATTCCTCCT 
GTGGACTACA GCTCTAAAGG AATGCCAAAT CCCCCAGACC CTGCATCATA TCATTCCTCT 
GCAAACAAAT ATAACTTGAC ACAACAGGAA CCCATGCGAC TTATTCACAA AGAGCTTCGG 
CTCCCTGAGG ATATTGAGCA TTCACATACC AGGCCAGCTG ACAACCAACA CCGTGACTGT 
TACAGGGAGG AGCCAGCTCA GTTTCCCCTT ACTGCACCTC CTCCTAAACC AGAGAGGAGC 
CATAGCCTTC GAGGTCATAA TCCAGATGTT TTAGAAAGAG ACCCTAGTAT CTTCTACCCC 
TACCAAACGC TTCAAGCCAA GAGACACAGC ACAATTAATG CAGTGTCACA ATATGATAAT 
CTAGCTGATT ACCATTCTAT ACCCCATCAT CGAACCACTA ACCAGTCTAC TCCAAATGCC 
TTTAACCTCC CACATGGACG GACCTATGCT ACTGCCCTGG GCCAGGGTGC CTTTCTAGCT 
GCTGAACTCG GCTTGCAAAG GCCTGAGACA AAAATCCATG TGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916222.1
CDS	2863..8850
Translation	MEGGTDAAAFAAFPSIHAVPSTSGRMATGGKLDELERIMKSSLHLEEDDFVPELPRSIHPRERPDWEETISAMARGADIPVPGEIPTMPTEIPLRSCGSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGSIQLTLTEDQNEVSRNGCESKELVFLVQIACQGRSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLSELPRSDSVKDSPELVTQMLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINVPAIAAAHVIKRYNAQAPDELSFEVGDIVSVIDMPPKELTTWWRGKHGFQVGFFPSECVELINDKVPQSMTNSVPKPVSPAHGMRPASWSYFPPYLEMDSLRPGTSCCSEVLSHSQLSSVSRKHGKLITFLRTFMKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSGAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_004916165.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916165.3

ORF Insert Sequence:

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGTCTCC 
CCTGCGCATG GAATGAGGCC GGCCTCCTGG AGCTACTTTC CCCCCTATTT GGAGATGGAT 
AGTTTAAGGC CTGGAACATC GTGCTGTTCT GAGGTATTAA GTCACTCGCA GCTAAGCTCA 
GTGTCCCGGA AGCACGGCAA GCTCATCACA TTTCTAAGAA CCTTCATGAA GTCCAGACCC 
AGCAAACAGA AACTGAAGCA GAGAGGGATC CTGAGGGAGC GAGTGTTTGG TTGTGACCTT 
GGGGAACATC TGCTTAACTC TGGTCAGGAT GTCCCCCAAG TCCTCAGGAG CTGCACAGAG 
TTTATAGAAA AACATGGCAT TGTTGATGGA ATCTACCGTC TGTCTGGGAT TGCATCTAAC 
ATCCAGAAAC TACGTCATGA ATTTGACTCT GAACAGATTC CAGACCTGAC TAAAGATGTG 
TACATCCAGG ACATTCACTG TGTTGGCTCT CTCTGTAAAC TCTACTTCCG GGAACTCCCA 
AACCCTCTGC TCACATATCA GTTGTATGAA AAGTTCTCTG ATGCTGTGTC AGCGGCTACT 
GATGAAGAGA GACTGGTGAA AATTCATGAT GTTATTCAAC AGCTTCCCCC ACCTCATTAC 
AGAACACTGG AGTTCCTTAT GCGCCACTTA TCAAGACTTG CGACATACTG CTCTATCACC 
AATATGCACA CTAAAAATTT GGCAATTGTC TGGGCCCCTA ACCTGCTCCG GTCAAAACAG 
ATTGAGTCTG CATGTTTTAG CGGTACAGCA GCTTTCATGG AAGTGCGCAT ACAGTCTGTG 
GTAGTTGAAT TTATTCTAAA CCATGTGGAG GTTCTCTTTA GTCCTAAACT TAGTTCTGTG 
ATAAGGGATG GTGCTGGTCA CACTTCTTTG TCTCGGCCCA AGTCACTGTT GGTGTCATCT 
CCATCTACAA AACTACTAAG CCTAGAGGAG GCTCAAGCAA GGACACAGGC CCAAATCAAT 
TCCCCTGTTG TGGCTGACAG CAAGTACATT GAGGTGGGAG AGGGACCAGC TGCTTTGCAG 
GGCAGATTCC ACACTGTAAT TGACTTCCCT TCTGAAAGGA AAAGACCGCC AACCAAAATG 
AAAAAATCTC CTGTTGGAAG CTGGCGTTCT TTTTTTAGCA TAGGCAAATC ATCATCTTCA 
GCCTCCATGT CTAAGCGGAA ACTTCAGCGT AATCCAAGTG AGCCTTTGGA AATGAAATCC 
ATGGCATTTG CAGGTGGAAG AGATAGCAGC ACCATGCGCT CAGCTAAAAG CGAGGAGTCT 
CTCAGTTCCT TGCATGCAGC AGATGGAGAG TCGAAGCTAT TTCGTCCACG TCGTCCTCGT 
TCCAGCAGCG ATGCTCTGTC TGCTTCATAT AATGGGGACC TTCTGGATAG CTGCAACCGT 
TGCAACTCCT ATGATAATCT GCCCCGAGAC CAAGACAGTG ATGGAGATGA GGGTTTGATT 
CATGTGCCTG CTATGCTGTC AGGTCGCTCT CCTGAGGATG AAGAACTCAG CCCACCAGAC 
ATTGGTATGG CCAGTTTGGA CTTTGACCCA ATGTCTTTTC AGTGCAGCCC TCCCCAGCTA 
GAATCTGAAG GACCAGACAT TTTTTTTCAG CTTGACATTT GTGCTAGCAA TGGCAAGGAT 
AAGGTGCAAA GTGGTGCACA AAACCTAGGC GGTGTCTCCA GCTCCGAACC TCACTCTCCT 
TTCCAAGAGA AAGTCGTTAG TCCATTTCTA TCGCCTGACC GCAGTTCTAG TACAGATAAA 
GTATCCAAAA CTACATCATT TGCAGAAAAA GTTGTGCAGG CCTTGTCACC TAAAGTGGGG 
CGCAAAGCAG TTCGATCTCC TCCATTAACC ATCTCAGAGC CTGTCACCAT CTCTCTCCCT 
AGTCGGGTAT CGGAAATGAT TGGAAGTATG CAAGGCAATA CTGCTGCTTC CCAGTCTTTA 
ATTTGGTACA GAGAAAACAG TTTAAATTCT AGAGAAACTG AAATGAATTA TGTTTGTAAA 
TCGGTGGATG CCAACCTTCC ATCCTTATTG CCAGCAACAG ATCCATGGTC TACAGTTACT 
CCTGTAATTA CTCAATGGAA CCCAGATGGA TTACAGAGAG AAGACAAAGC AGGACCATTT 
ACTCATGGCT TTCCCCAGCC AAATAGTGAC TTGGAACAAG TAGAAAACTT CAACCTGGAA 
CTTAGCACAA CAAATGTTGC TGCAGCATAT CAGAAAGAAG ATACAGAATT GTCGAGAGCA 
AATCAGAACA AATCCCACTC TTCTGTTTCT CCCCCTCAAG GAGCTTCTGC TTCAGAATCT 
CCACAGGAGC TAGCCCCTGT CAGTAGTGTT TCTATCATTC CACCTCCACC TCCTCCAAAG 
AATCCTGCAC GCATGCTGGC ATTAGCACTG GCTGAATCTG CTCAACAGGC ATCTGCTAAT 
AAAAGACCTC TTTATATGCA GTTCATGGAT CTACCCCCCC ATCCTCCTCC ACCATCAGTA 
CTAGAGGACA AACCACTTCT TGAGTTATCT CCAAAAATGT CTCCAGAGCA CCTCGTATCT 
GGTCCTGCAA CACCAGCGAC CAGTCCTCCA GTCATATCCA ATAAAACATC TCCAGCTACT 
CCACCAAGCA CAACTTCATC ATTTTCTATC ACAACAATTC ATCACAGTCC TGTGAAAAAC 
CATAGTCCTC TGGCAGGACA GATGCCTACA GCAGTCACTC CTGGACTCAG TCCTTCATCA 
AACCAGGTTC CTGTATCCTT TCTATCTGAT GAAATGCTAC CTTCTGAAAC AAATAAAATG 
AATTCTGTCA TACTGAGTGA AGCATTTACA ACAGGAATTC CTGTTACTCC ACCATCTGAA 
AAACCCAAGG AAATTTCTAG ACCACCACAT CTTCACCAGC GTTCTGAATC CTTTCCTTCA 
CATCCTGCAT ATAGCAATGC AAAGCCCACT CCACCAGTTA GAACAATGGA TAGTCGACTG 
GCAACTGCCA TGCATTCCAA CTTCAATGAT GCCATCACTG CTAATAACTA CCACTCCTTC 
CTGAACACCA TGATGCTTCC TTCTTCTCTA GAGGATGTGC TACCCAGACA CAACTATTCT 
CATTCTTCTC ATCTCAAGAC AGAAAATATA GATGAACAGG GAGTTAATTA CAGACAACCT 
TATTTATCCA ACACCAAGCA AGAGGACCCA GCTGATGTGG GAGATTTATA CAGACAGCAA 
TATATTTCTA CTGGAAAGTC TGAGAATGCT GAGATAGGAA ACACTTACCG GCACCCATAT 
CCTTCCAATA CTGAGCCAGA AAGTCTAAAG GAGCCACTAG ATCCTTTTCT GCATCACAAG 
TCAGCAGTTG CTCCAAAGAC ATATAGAGCA GAAACACTTC TACCACATAT GCCTCCTCAA 
GTTTATGGTT CAAGATGTGA TACTCCATCC TCAACATTTT ATGGCACCTA CATAAATCAG 
GCCAAACACC CCCGCACCAG GAACAGACCT GATTATATGT CCTCTATGAG CCCAGGTCTC 
CGTAGCTACT CAGAAGACAC ATCTTCCTAC CCTACTATTC GAAGGGTACA ATCTCTGCAT 
GTGCCTATGC AACCTCCGCC ACCTCCTATC CGCACTGTTC CAATTTCAAG AACAGAGGTT 
CCCCCAGATG ATGATCCAGC ATACTGCCCA AGACCAGTTT ATCAATACAA ACAATGCCCC 
CAATTTAACT CTCAGTCAGA TTACCATGTT ACCCAGCTGC AGCCTTACTT TGAAAATGGA 
CGAGTTCATT ATCGCTACAG TCCCTATTCA GGGGGCCCAA GTTATTTTTC AGCAGATAGC 
AATTTCTATG ATATGGACCC CTACAGCACC TTACGTCTGC GACAACTTCC TCTCTACCCA 
AGCCGGGACT TTGCTTCTTA CACCACAAGA TTGCAGCCCA AAAACACATA TCGGTCACAA 
GGATTGCCCC CATATCCCAG AGGAACTCTA CGGGAACACA ATTTTATCAG TAGAGATGTT 
CCTCCTGCAC TACCACCTGA ACATCCACGA CCTCTTCACA TTTCTTGGGA CATGGAAGAC 
ATGGACAGAT ACAGACTGCA ATCCCTGCGA CGAGAGAACA GGGCACGTCA GAGAGCTAAA 
GGACCAGTAA TGTCTCAGTA TGATAATGTT ACACCAACCT TAGTGGAGGA TGTAGCAGGC 
TTAGATGTCA TACATCTCCG AAGCCGTTCT GATCCTGGTA AGACTCCTGG TCTTTTAAGT 
GTGGCTGAGA ACAAAGACGT CCGTTACCCA GGAAGGGCAG AAGGTGATGA GCATACAACA 
TATCCACCTC CTGTTTTTGG AAATGGCCAT CAAGATAAGC CATCTCTTCC TCAAAAGCAA 
AGTGGATCAA GCCGGGTTCG AATGCAACAT GAAATATCTA CTGAGCAGCG GACTCAACCT 
TCTCAAGATA CTTTGCATAG GCAACCCACA GAGGGACGAA ATGGTCCTCC AATTCCTCCT 
GTGGACTACA GCTCTAAAGG AATGCCAAAT CCCCCAGACC CTGCATCATA TCATTCCTCT 
GCAAACAAAT ATAACTTGAC ACAACAGGAA CCCATGCGAC TTATTCACAA AGAGCTTCGG 
CTCCCTGAGG ATATTGAGCA TTCACATACC AGGCCAGCTG ACAACCAACA CCGTGACTGT 
TACAGGGAGG AGCCAGCTCA GTTTCCCCTT ACTGCACCTC CTCCTAAACC AGAGAGGAGC 
CATAGCCTTC GAGGTCATAA TCCAGATGTT TTAGAAAGAG ACCCTAGTAT CTTCTACCCC 
TACCAAACGC TTCAAGCCAA GAGACACAGC ACAATTAATG CAGTGTCACA ATATGATAAT 
CTAGCTGATT ACCATTCTAT ACCCCATCAT CGAACCACTA ACCAGTCTAC TCCAAATGCC 
TTTAACCTCC CACATGGACG GACCTATGCT ACTGCCCTGG GCCAGGGTGC CTTTCTAGCT 
GCTGAACTCG GCTTGCAAAG GCCTGAGACA AAAATCCATG TGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_004916164.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916221.1 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X1, mRNA.

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CloneID	OXa13195
Clone ID Related Accession (Same CDS sequence)	XM_004916165.3 , XM_004916164.3
Accession Version	XM_004916164.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916164.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGTCTCC 
CCTGCGCATG GAATGAGGCC GGCCTCCTGG AGCTACTTTC CCCCCTATTT GGAGATGGAT 
AGTTTAAGGC CTGGAACATC GTGCTGTTCT GAGGTATTAA GTCACTCGCA GCTAAGCTCA 
GTGTCCCGGA AGCACGGCAA GCTCATCACA TTTCTAAGAA CCTTCATGAA GTCCAGACCC 
AGCAAACAGA AACTGAAGCA GAGAGGGATC CTGAGGGAGC GAGTGTTTGG TTGTGACCTT 
GGGGAACATC TGCTTAACTC TGGTCAGGAT GTCCCCCAAG TCCTCAGGAG CTGCACAGAG 
TTTATAGAAA AACATGGCAT TGTTGATGGA ATCTACCGTC TGTCTGGGAT TGCATCTAAC 
ATCCAGAAAC TACGTCATGA ATTTGACTCT GAACAGATTC CAGACCTGAC TAAAGATGTG 
TACATCCAGG ACATTCACTG TGTTGGCTCT CTCTGTAAAC TCTACTTCCG GGAACTCCCA 
AACCCTCTGC TCACATATCA GTTGTATGAA AAGTTCTCTG ATGCTGTGTC AGCGGCTACT 
GATGAAGAGA GACTGGTGAA AATTCATGAT GTTATTCAAC AGCTTCCCCC ACCTCATTAC 
AGAACACTGG AGTTCCTTAT GCGCCACTTA TCAAGACTTG CGACATACTG CTCTATCACC 
AATATGCACA CTAAAAATTT GGCAATTGTC TGGGCCCCTA ACCTGCTCCG GTCAAAACAG 
ATTGAGTCTG CATGTTTTAG CGGTACAGCA GCTTTCATGG AAGTGCGCAT ACAGTCTGTG 
GTAGTTGAAT TTATTCTAAA CCATGTGGAG GTTCTCTTTA GTCCTAAACT TAGTTCTGTG 
ATAAGGGATG GTGCTGGTCA CACTTCTTTG TCTCGGCCCA AGTCACTGTT GGTGTCATCT 
CCATCTACAA AACTACTAAG CCTAGAGGAG GCTCAAGCAA GGACACAGGC CCAAATCAAT 
TCCCCTGTTG TGGCTGACAG CAAGTACATT GAGGTGGGAG AGGGACCAGC TGCTTTGCAG 
GGCAGATTCC ACACTGTAAT TGACTTCCCT TCTGAAAGGA AAAGACCGCC AACCAAAATG 
AAAAAATCTC CTGTTGGAAG CTGGCGTTCT TTTTTTAGCA TAGGCAAATC ATCATCTTCA 
GCCTCCATGT CTAAGCGGAA ACTTCAGCGT AATCCAAGTG AGCCTTTGGA AATGAAATCC 
ATGGCATTTG CAGGTGGAAG AGATAGCAGC ACCATGCGCT CAGCTAAAAG CGAGGAGTCT 
CTCAGTTCCT TGCATGCAGC AGATGGAGAG TCGAAGCTAT TTCGTCCACG TCGTCCTCGT 
TCCAGCAGCG ATGCTCTGTC TGCTTCATAT AATGGGGACC TTCTGGATAG CTGCAACCGT 
TGCAACTCCT ATGATAATCT GCCCCGAGAC CAAGACAGTG ATGGAGATGA GGGTTTGATT 
CATGTGCCTG CTATGCTGTC AGGTCGCTCT CCTGAGGATG AAGAACTCAG CCCACCAGAC 
ATTGGTATGG CCAGTTTGGA CTTTGACCCA ATGTCTTTTC AGTGCAGCCC TCCCCAGCTA 
GAATCTGAAG GACCAGACAT TTTTTTTCAG CTTGACATTT GTGCTAGCAA TGGCAAGGAT 
AAGGTGCAAA GTGGTGCACA AAACCTAGGC GGTGTCTCCA GCTCCGAACC TCACTCTCCT 
TTCCAAGAGA AAGTCGTTAG TCCATTTCTA TCGCCTGACC GCAGTTCTAG TACAGATAAA 
GTATCCAAAA CTACATCATT TGCAGAAAAA GTTGTGCAGG CCTTGTCACC TAAAGTGGGG 
CGCAAAGCAG TTCGATCTCC TCCATTAACC ATCTCAGAGC CTGTCACCAT CTCTCTCCCT 
AGTCGGGTAT CGGAAATGAT TGGAAGTATG CAAGGCAATA CTGCTGCTTC CCAGTCTTTA 
ATTTGGTACA GAGAAAACAG TTTAAATTCT AGAGAAACTG AAATGAATTA TGTTTGTAAA 
TCGGTGGATG CCAACCTTCC ATCCTTATTG CCAGCAACAG ATCCATGGTC TACAGTTACT 
CCTGTAATTA CTCAATGGAA CCCAGATGGA TTACAGAGAG AAGACAAAGC AGGACCATTT 
ACTCATGGCT TTCCCCAGCC AAATAGTGAC TTGGAACAAG TAGAAAACTT CAACCTGGAA 
CTTAGCACAA CAAATGTTGC TGCAGCATAT CAGAAAGAAG ATACAGAATT GTCGAGAGCA 
AATCAGAACA AATCCCACTC TTCTGTTTCT CCCCCTCAAG GAGCTTCTGC TTCAGAATCT 
CCACAGGAGC TAGCCCCTGT CAGTAGTGTT TCTATCATTC CACCTCCACC TCCTCCAAAG 
AATCCTGCAC GCATGCTGGC ATTAGCACTG GCTGAATCTG CTCAACAGGC ATCTGCTAAT 
AAAAGACCTC TTTATATGCA GTTCATGGAT CTACCCCCCC ATCCTCCTCC ACCATCAGTA 
CTAGAGGACA AACCACTTCT TGAGTTATCT CCAAAAATGT CTCCAGAGCA CCTCGTATCT 
GGTCCTGCAA CACCAGCGAC CAGTCCTCCA GTCATATCCA ATAAAACATC TCCAGCTACT 
CCACCAAGCA CAACTTCATC ATTTTCTATC ACAACAATTC ATCACAGTCC TGTGAAAAAC 
CATAGTCCTC TGGCAGGACA GATGCCTACA GCAGTCACTC CTGGACTCAG TCCTTCATCA 
AACCAGGTTC CTGTATCCTT TCTATCTGAT GAAATGCTAC CTTCTGAAAC AAATAAAATG 
AATTCTGTCA TACTGAGTGA AGCATTTACA ACAGGAATTC CTGTTACTCC ACCATCTGAA 
AAACCCAAGG AAATTTCTAG ACCACCACAT CTTCACCAGC GTTCTGAATC CTTTCCTTCA 
CATCCTGCAT ATAGCAATGC AAAGCCCACT CCACCAGTTA GAACAATGGA TAGTCGACTG 
GCAACTGCCA TGCATTCCAA CTTCAATGAT GCCATCACTG CTAATAACTA CCACTCCTTC 
CTGAACACCA TGATGCTTCC TTCTTCTCTA GAGGATGTGC TACCCAGACA CAACTATTCT 
CATTCTTCTC ATCTCAAGAC AGAAAATATA GATGAACAGG GAGTTAATTA CAGACAACCT 
TATTTATCCA ACACCAAGCA AGAGGACCCA GCTGATGTGG GAGATTTATA CAGACAGCAA 
TATATTTCTA CTGGAAAGTC TGAGAATGCT GAGATAGGAA ACACTTACCG GCACCCATAT 
CCTTCCAATA CTGAGCCAGA AAGTCTAAAG GAGCCACTAG ATCCTTTTCT GCATCACAAG 
TCAGCAGTTG CTCCAAAGAC ATATAGAGCA GAAACACTTC TACCACATAT GCCTCCTCAA 
GTTTATGGTT CAAGATGTGA TACTCCATCC TCAACATTTT ATGGCACCTA CATAAATCAG 
GCCAAACACC CCCGCACCAG GAACAGACCT GATTATATGT CCTCTATGAG CCCAGGTCTC 
CGTAGCTACT CAGAAGACAC ATCTTCCTAC CCTACTATTC GAAGGGTACA ATCTCTGCAT 
GTGCCTATGC AACCTCCGCC ACCTCCTATC CGCACTGTTC CAATTTCAAG AACAGAGGTT 
CCCCCAGATG ATGATCCAGC ATACTGCCCA AGACCAGTTT ATCAATACAA ACAATGCCCC 
CAATTTAACT CTCAGTCAGA TTACCATGTT ACCCAGCTGC AGCCTTACTT TGAAAATGGA 
CGAGTTCATT ATCGCTACAG TCCCTATTCA GGGGGCCCAA GTTATTTTTC AGCAGATAGC 
AATTTCTATG ATATGGACCC CTACAGCACC TTACGTCTGC GACAACTTCC TCTCTACCCA 
AGCCGGGACT TTGCTTCTTA CACCACAAGA TTGCAGCCCA AAAACACATA TCGGTCACAA 
GGATTGCCCC CATATCCCAG AGGAACTCTA CGGGAACACA ATTTTATCAG TAGAGATGTT 
CCTCCTGCAC TACCACCTGA ACATCCACGA CCTCTTCACA TTTCTTGGGA CATGGAAGAC 
ATGGACAGAT ACAGACTGCA ATCCCTGCGA CGAGAGAACA GGGCACGTCA GAGAGCTAAA 
GGACCAGTAA TGTCTCAGTA TGATAATGTT ACACCAACCT TAGTGGAGGA TGTAGCAGGC 
TTAGATGTCA TACATCTCCG AAGCCGTTCT GATCCTGGTA AGACTCCTGG TCTTTTAAGT 
GTGGCTGAGA ACAAAGACGT CCGTTACCCA GGAAGGGCAG AAGGTGATGA GCATACAACA 
TATCCACCTC CTGTTTTTGG AAATGGCCAT CAAGATAAGC CATCTCTTCC TCAAAAGCAA 
AGTGGATCAA GCCGGGTTCG AATGCAACAT GAAATATCTA CTGAGCAGCG GACTCAACCT 
TCTCAAGATA CTTTGCATAG GCAACCCACA GAGGGACGAA ATGGTCCTCC AATTCCTCCT 
GTGGACTACA GCTCTAAAGG AATGCCAAAT CCCCCAGACC CTGCATCATA TCATTCCTCT 
GCAAACAAAT ATAACTTGAC ACAACAGGAA CCCATGCGAC TTATTCACAA AGAGCTTCGG 
CTCCCTGAGG ATATTGAGCA TTCACATACC AGGCCAGCTG ACAACCAACA CCGTGACTGT 
TACAGGGAGG AGCCAGCTCA GTTTCCCCTT ACTGCACCTC CTCCTAAACC AGAGAGGAGC 
CATAGCCTTC GAGGTCATAA TCCAGATGTT TTAGAAAGAG ACCCTAGTAT CTTCTACCCC 
TACCAAACGC TTCAAGCCAA GAGACACAGC ACAATTAATG CAGTGTCACA ATATGATAAT 
CTAGCTGATT ACCATTCTAT ACCCCATCAT CGAACCACTA ACCAGTCTAC TCCAAATGCC 
TTTAACCTCC CACATGGACG GACCTATGCT ACTGCCCTGG GCCAGGGTGC CTTTCTAGCT 
GCTGAACTCG GCTTGCAAAG GCCTGAGACA AAAATCCATG TGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916221.1
CDS	2903..8890
Translation	MEGGTDAAAFAAFPSIHAVPSTSGRMATGGKLDELERIMKSSLHLEEDDFVPELPRSIHPRERPDWEETISAMARGADIPVPGEIPTMPTEIPLRSCGSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGSIQLTLTEDQNEVSRNGCESKELVFLVQIACQGRSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLSELPRSDSVKDSPELVTQMLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINVPAIAAAHVIKRYNAQAPDELSFEVGDIVSVIDMPPKELTTWWRGKHGFQVGFFPSECVELINDKVPQSMTNSVPKPVSPAHGMRPASWSYFPPYLEMDSLRPGTSCCSEVLSHSQLSSVSRKHGKLITFLRTFMKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSGAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_004916164.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916164.3

ORF Insert Sequence:

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGTCTCC 
CCTGCGCATG GAATGAGGCC GGCCTCCTGG AGCTACTTTC CCCCCTATTT GGAGATGGAT 
AGTTTAAGGC CTGGAACATC GTGCTGTTCT GAGGTATTAA GTCACTCGCA GCTAAGCTCA 
GTGTCCCGGA AGCACGGCAA GCTCATCACA TTTCTAAGAA CCTTCATGAA GTCCAGACCC 
AGCAAACAGA AACTGAAGCA GAGAGGGATC CTGAGGGAGC GAGTGTTTGG TTGTGACCTT 
GGGGAACATC TGCTTAACTC TGGTCAGGAT GTCCCCCAAG TCCTCAGGAG CTGCACAGAG 
TTTATAGAAA AACATGGCAT TGTTGATGGA ATCTACCGTC TGTCTGGGAT TGCATCTAAC 
ATCCAGAAAC TACGTCATGA ATTTGACTCT GAACAGATTC CAGACCTGAC TAAAGATGTG 
TACATCCAGG ACATTCACTG TGTTGGCTCT CTCTGTAAAC TCTACTTCCG GGAACTCCCA 
AACCCTCTGC TCACATATCA GTTGTATGAA AAGTTCTCTG ATGCTGTGTC AGCGGCTACT 
GATGAAGAGA GACTGGTGAA AATTCATGAT GTTATTCAAC AGCTTCCCCC ACCTCATTAC 
AGAACACTGG AGTTCCTTAT GCGCCACTTA TCAAGACTTG CGACATACTG CTCTATCACC 
AATATGCACA CTAAAAATTT GGCAATTGTC TGGGCCCCTA ACCTGCTCCG GTCAAAACAG 
ATTGAGTCTG CATGTTTTAG CGGTACAGCA GCTTTCATGG AAGTGCGCAT ACAGTCTGTG 
GTAGTTGAAT TTATTCTAAA CCATGTGGAG GTTCTCTTTA GTCCTAAACT TAGTTCTGTG 
ATAAGGGATG GTGCTGGTCA CACTTCTTTG TCTCGGCCCA AGTCACTGTT GGTGTCATCT 
CCATCTACAA AACTACTAAG CCTAGAGGAG GCTCAAGCAA GGACACAGGC CCAAATCAAT 
TCCCCTGTTG TGGCTGACAG CAAGTACATT GAGGTGGGAG AGGGACCAGC TGCTTTGCAG 
GGCAGATTCC ACACTGTAAT TGACTTCCCT TCTGAAAGGA AAAGACCGCC AACCAAAATG 
AAAAAATCTC CTGTTGGAAG CTGGCGTTCT TTTTTTAGCA TAGGCAAATC ATCATCTTCA 
GCCTCCATGT CTAAGCGGAA ACTTCAGCGT AATCCAAGTG AGCCTTTGGA AATGAAATCC 
ATGGCATTTG CAGGTGGAAG AGATAGCAGC ACCATGCGCT CAGCTAAAAG CGAGGAGTCT 
CTCAGTTCCT TGCATGCAGC AGATGGAGAG TCGAAGCTAT TTCGTCCACG TCGTCCTCGT 
TCCAGCAGCG ATGCTCTGTC TGCTTCATAT AATGGGGACC TTCTGGATAG CTGCAACCGT 
TGCAACTCCT ATGATAATCT GCCCCGAGAC CAAGACAGTG ATGGAGATGA GGGTTTGATT 
CATGTGCCTG CTATGCTGTC AGGTCGCTCT CCTGAGGATG AAGAACTCAG CCCACCAGAC 
ATTGGTATGG CCAGTTTGGA CTTTGACCCA ATGTCTTTTC AGTGCAGCCC TCCCCAGCTA 
GAATCTGAAG GACCAGACAT TTTTTTTCAG CTTGACATTT GTGCTAGCAA TGGCAAGGAT 
AAGGTGCAAA GTGGTGCACA AAACCTAGGC GGTGTCTCCA GCTCCGAACC TCACTCTCCT 
TTCCAAGAGA AAGTCGTTAG TCCATTTCTA TCGCCTGACC GCAGTTCTAG TACAGATAAA 
GTATCCAAAA CTACATCATT TGCAGAAAAA GTTGTGCAGG CCTTGTCACC TAAAGTGGGG 
CGCAAAGCAG TTCGATCTCC TCCATTAACC ATCTCAGAGC CTGTCACCAT CTCTCTCCCT 
AGTCGGGTAT CGGAAATGAT TGGAAGTATG CAAGGCAATA CTGCTGCTTC CCAGTCTTTA 
ATTTGGTACA GAGAAAACAG TTTAAATTCT AGAGAAACTG AAATGAATTA TGTTTGTAAA 
TCGGTGGATG CCAACCTTCC ATCCTTATTG CCAGCAACAG ATCCATGGTC TACAGTTACT 
CCTGTAATTA CTCAATGGAA CCCAGATGGA TTACAGAGAG AAGACAAAGC AGGACCATTT 
ACTCATGGCT TTCCCCAGCC AAATAGTGAC TTGGAACAAG TAGAAAACTT CAACCTGGAA 
CTTAGCACAA CAAATGTTGC TGCAGCATAT CAGAAAGAAG ATACAGAATT GTCGAGAGCA 
AATCAGAACA AATCCCACTC TTCTGTTTCT CCCCCTCAAG GAGCTTCTGC TTCAGAATCT 
CCACAGGAGC TAGCCCCTGT CAGTAGTGTT TCTATCATTC CACCTCCACC TCCTCCAAAG 
AATCCTGCAC GCATGCTGGC ATTAGCACTG GCTGAATCTG CTCAACAGGC ATCTGCTAAT 
AAAAGACCTC TTTATATGCA GTTCATGGAT CTACCCCCCC ATCCTCCTCC ACCATCAGTA 
CTAGAGGACA AACCACTTCT TGAGTTATCT CCAAAAATGT CTCCAGAGCA CCTCGTATCT 
GGTCCTGCAA CACCAGCGAC CAGTCCTCCA GTCATATCCA ATAAAACATC TCCAGCTACT 
CCACCAAGCA CAACTTCATC ATTTTCTATC ACAACAATTC ATCACAGTCC TGTGAAAAAC 
CATAGTCCTC TGGCAGGACA GATGCCTACA GCAGTCACTC CTGGACTCAG TCCTTCATCA 
AACCAGGTTC CTGTATCCTT TCTATCTGAT GAAATGCTAC CTTCTGAAAC AAATAAAATG 
AATTCTGTCA TACTGAGTGA AGCATTTACA ACAGGAATTC CTGTTACTCC ACCATCTGAA 
AAACCCAAGG AAATTTCTAG ACCACCACAT CTTCACCAGC GTTCTGAATC CTTTCCTTCA 
CATCCTGCAT ATAGCAATGC AAAGCCCACT CCACCAGTTA GAACAATGGA TAGTCGACTG 
GCAACTGCCA TGCATTCCAA CTTCAATGAT GCCATCACTG CTAATAACTA CCACTCCTTC 
CTGAACACCA TGATGCTTCC TTCTTCTCTA GAGGATGTGC TACCCAGACA CAACTATTCT 
CATTCTTCTC ATCTCAAGAC AGAAAATATA GATGAACAGG GAGTTAATTA CAGACAACCT 
TATTTATCCA ACACCAAGCA AGAGGACCCA GCTGATGTGG GAGATTTATA CAGACAGCAA 
TATATTTCTA CTGGAAAGTC TGAGAATGCT GAGATAGGAA ACACTTACCG GCACCCATAT 
CCTTCCAATA CTGAGCCAGA AAGTCTAAAG GAGCCACTAG ATCCTTTTCT GCATCACAAG 
TCAGCAGTTG CTCCAAAGAC ATATAGAGCA GAAACACTTC TACCACATAT GCCTCCTCAA 
GTTTATGGTT CAAGATGTGA TACTCCATCC TCAACATTTT ATGGCACCTA CATAAATCAG 
GCCAAACACC CCCGCACCAG GAACAGACCT GATTATATGT CCTCTATGAG CCCAGGTCTC 
CGTAGCTACT CAGAAGACAC ATCTTCCTAC CCTACTATTC GAAGGGTACA ATCTCTGCAT 
GTGCCTATGC AACCTCCGCC ACCTCCTATC CGCACTGTTC CAATTTCAAG AACAGAGGTT 
CCCCCAGATG ATGATCCAGC ATACTGCCCA AGACCAGTTT ATCAATACAA ACAATGCCCC 
CAATTTAACT CTCAGTCAGA TTACCATGTT ACCCAGCTGC AGCCTTACTT TGAAAATGGA 
CGAGTTCATT ATCGCTACAG TCCCTATTCA GGGGGCCCAA GTTATTTTTC AGCAGATAGC 
AATTTCTATG ATATGGACCC CTACAGCACC TTACGTCTGC GACAACTTCC TCTCTACCCA 
AGCCGGGACT TTGCTTCTTA CACCACAAGA TTGCAGCCCA AAAACACATA TCGGTCACAA 
GGATTGCCCC CATATCCCAG AGGAACTCTA CGGGAACACA ATTTTATCAG TAGAGATGTT 
CCTCCTGCAC TACCACCTGA ACATCCACGA CCTCTTCACA TTTCTTGGGA CATGGAAGAC 
ATGGACAGAT ACAGACTGCA ATCCCTGCGA CGAGAGAACA GGGCACGTCA GAGAGCTAAA 
GGACCAGTAA TGTCTCAGTA TGATAATGTT ACACCAACCT TAGTGGAGGA TGTAGCAGGC 
TTAGATGTCA TACATCTCCG AAGCCGTTCT GATCCTGGTA AGACTCCTGG TCTTTTAAGT 
GTGGCTGAGA ACAAAGACGT CCGTTACCCA GGAAGGGCAG AAGGTGATGA GCATACAACA 
TATCCACCTC CTGTTTTTGG AAATGGCCAT CAAGATAAGC CATCTCTTCC TCAAAAGCAA 
AGTGGATCAA GCCGGGTTCG AATGCAACAT GAAATATCTA CTGAGCAGCG GACTCAACCT 
TCTCAAGATA CTTTGCATAG GCAACCCACA GAGGGACGAA ATGGTCCTCC AATTCCTCCT 
GTGGACTACA GCTCTAAAGG AATGCCAAAT CCCCCAGACC CTGCATCATA TCATTCCTCT 
GCAAACAAAT ATAACTTGAC ACAACAGGAA CCCATGCGAC TTATTCACAA AGAGCTTCGG 
CTCCCTGAGG ATATTGAGCA TTCACATACC AGGCCAGCTG ACAACCAACA CCGTGACTGT 
TACAGGGAGG AGCCAGCTCA GTTTCCCCTT ACTGCACCTC CTCCTAAACC AGAGAGGAGC 
CATAGCCTTC GAGGTCATAA TCCAGATGTT TTAGAAAGAG ACCCTAGTAT CTTCTACCCC 
TACCAAACGC TTCAAGCCAA GAGACACAGC ACAATTAATG CAGTGTCACA ATATGATAAT 
CTAGCTGATT ACCATTCTAT ACCCCATCAT CGAACCACTA ACCAGTCTAC TCCAAATGCC 
TTTAACCTCC CACATGGACG GACCTATGCT ACTGCCCTGG GCCAGGGTGC CTTTCTAGCT 
GCTGAACTCG GCTTGCAAAG GCCTGAGACA AAAATCCATG TGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_004916166.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916223.1 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X4, mRNA.

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CloneID	OXa13196
Clone ID Related Accession (Same CDS sequence)	XM_004916166.3
Accession Version	XM_004916166.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916166.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGTCTCC 
CCTGCGCATG GAATGAGGCC GGCCTCCTGG AGCTACTTTC CCCCCTTGTC CCGGAAGCAC 
GGCAAGCTCA TCACATTTCT AAGAACCTTC ATGAAGTCCA GACCCAGCAA ACAGAAACTG 
AAGCAGAGAG GGATCCTGAG GGAGCGAGTG TTTGGTTGTG ACCTTGGGGA ACATCTGCTT 
AACTCTGGTC AGGATGTCCC CCAAGTCCTC AGGAGCTGCA CAGAGTTTAT AGAAAAACAT 
GGCATTGTTG ATGGAATCTA CCGTCTGTCT GGGATTGCAT CTAACATCCA GAAACTACGT 
CATGAATTTG ACTCTGAACA GATTCCAGAC CTGACTAAAG ATGTGTACAT CCAGGACATT 
CACTGTGTTG GCTCTCTCTG TAAACTCTAC TTCCGGGAAC TCCCAAACCC TCTGCTCACA 
TATCAGTTGT ATGAAAAGTT CTCTGATGCT GTGTCAGCGG CTACTGATGA AGAGAGACTG 
GTGAAAATTC ATGATGTTAT TCAACAGCTT CCCCCACCTC ATTACAGAAC ACTGGAGTTC 
CTTATGCGCC ACTTATCAAG ACTTGCGACA TACTGCTCTA TCACCAATAT GCACACTAAA 
AATTTGGCAA TTGTCTGGGC CCCTAACCTG CTCCGGTCAA AACAGATTGA GTCTGCATGT 
TTTAGCGGTA CAGCAGCTTT CATGGAAGTG CGCATACAGT CTGTGGTAGT TGAATTTATT 
CTAAACCATG TGGAGGTTCT CTTTAGTCCT AAACTTAGTT CTGTGATAAG GGATGGTGCT 
GGTCACACTT CTTTGTCTCG GCCCAAGTCA CTGTTGGTGT CATCTCCATC TACAAAACTA 
CTAAGCCTAG AGGAGGCTCA AGCAAGGACA CAGGCCCAAA TCAATTCCCC TGTTGTGGCT 
GACAGCAAGT ACATTGAGGT GGGAGAGGGA CCAGCTGCTT TGCAGGGCAG ATTCCACACT 
GTAATTGACT TCCCTTCTGA AAGGAAAAGA CCGCCAACCA AAATGAAAAA ATCTCCTGTT 
GGAAGCTGGC GTTCTTTTTT TAGCATAGGC AAATCATCAT CTTCAGCCTC CATGTCTAAG 
CGGAAACTTC AGCGTAATCC AAGTGAGCCT TTGGAAATGA AATCCATGGC ATTTGCAGGT 
GGAAGAGATA GCAGCACCAT GCGCTCAGCT AAAAGCGAGG AGTCTCTCAG TTCCTTGCAT 
GCAGCAGATG GAGAGTCGAA GCTATTTCGT CCACGTCGTC CTCGTTCCAG CAGCGATGCT 
CTGTCTGCTT CATATAATGG GGACCTTCTG GATAGCTGCA ACCGTTGCAA CTCCTATGAT 
AATCTGCCCC GAGACCAAGA CAGTGATGGA GATGAGGGTT TGATTCATGT GCCTGCTATG 
CTGTCAGGTC GCTCTCCTGA GGATGAAGAA CTCAGCCCAC CAGACATTGG TATGGCCAGT 
TTGGACTTTG ACCCAATGTC TTTTCAGTGC AGCCCTCCCC AGCTAGAATC TGAAGGACCA 
GACATTTTTT TTCAGCTTGA CATTTGTGCT AGCAATGGCA AGGATAAGGT GCAAAGTGGT 
GCACAAAACC TAGGCGGTGT CTCCAGCTCC GAACCTCACT CTCCTTTCCA AGAGAAAGTC 
GTTAGTCCAT TTCTATCGCC TGACCGCAGT TCTAGTACAG ATAAAGTATC CAAAACTACA 
TCATTTGCAG AAAAAGTTGT GCAGGCCTTG TCACCTAAAG TGGGGCGCAA AGCAGTTCGA 
TCTCCTCCAT TAACCATCTC AGAGCCTGTC ACCATCTCTC TCCCTAGTCG GGTATCGGAA 
ATGATTGGAA GTATGCAAGG CAATACTGCT GCTTCCCAGT CTTTAATTTG GTACAGAGAA 
AACAGTTTAA ATTCTAGAGA AACTGAAATG AATTATGTTT GTAAATCGGT GGATGCCAAC 
CTTCCATCCT TATTGCCAGC AACAGATCCA TGGTCTACAG TTACTCCTGT AATTACTCAA 
TGGAACCCAG ATGGATTACA GAGAGAAGAC AAAGCAGGAC CATTTACTCA TGGCTTTCCC 
CAGCCAAATA GTGACTTGGA ACAAGTAGAA AACTTCAACC TGGAACTTAG CACAACAAAT 
GTTGCTGCAG CATATCAGAA AGAAGATACA GAATTGTCGA GAGCAAATCA GAACAAATCC 
CACTCTTCTG TTTCTCCCCC TCAAGGAGCT TCTGCTTCAG AATCTCCACA GGAGCTAGCC 
CCTGTCAGTA GTGTTTCTAT CATTCCACCT CCACCTCCTC CAAAGAATCC TGCACGCATG 
CTGGCATTAG CACTGGCTGA ATCTGCTCAA CAGGCATCTG CTAATAAAAG ACCTCTTTAT 
ATGCAGTTCA TGGATCTACC CCCCCATCCT CCTCCACCAT CAGTACTAGA GGACAAACCA 
CTTCTTGAGT TATCTCCAAA AATGTCTCCA GAGCACCTCG TATCTGGTCC TGCAACACCA 
GCGACCAGTC CTCCAGTCAT ATCCAATAAA ACATCTCCAG CTACTCCACC AAGCACAACT 
TCATCATTTT CTATCACAAC AATTCATCAC AGTCCTGTGA AAAACCATAG TCCTCTGGCA 
GGACAGATGC CTACAGCAGT CACTCCTGGA CTCAGTCCTT CATCAAACCA GGTTCCTGTA 
TCCTTTCTAT CTGATGAAAT GCTACCTTCT GAAACAAATA AAATGAATTC TGTCATACTG 
AGTGAAGCAT TTACAACAGG AATTCCTGTT ACTCCACCAT CTGAAAAACC CAAGGAAATT 
TCTAGACCAC CACATCTTCA CCAGCGTTCT GAATCCTTTC CTTCACATCC TGCATATAGC 
AATGCAAAGC CCACTCCACC AGTTAGAACA ATGGATAGTC GACTGGCAAC TGCCATGCAT 
TCCAACTTCA ATGATGCCAT CACTGCTAAT AACTACCACT CCTTCCTGAA CACCATGATG 
CTTCCTTCTT CTCTAGAGGA TGTGCTACCC AGACACAACT ATTCTCATTC TTCTCATCTC 
AAGACAGAAA ATATAGATGA ACAGGGAGTT AATTACAGAC AACCTTATTT ATCCAACACC 
AAGCAAGAGG ACCCAGCTGA TGTGGGAGAT TTATACAGAC AGCAATATAT TTCTACTGGA 
AAGTCTGAGA ATGCTGAGAT AGGAAACACT TACCGGCACC CATATCCTTC CAATACTGAG 
CCAGAAAGTC TAAAGGAGCC ACTAGATCCT TTTCTGCATC ACAAGTCAGC AGTTGCTCCA 
AAGACATATA GAGCAGAAAC ACTTCTACCA CATATGCCTC CTCAAGTTTA TGGTTCAAGA 
TGTGATACTC CATCCTCAAC ATTTTATGGC ACCTACATAA ATCAGGCCAA ACACCCCCGC 
ACCAGGAACA GACCTGATTA TATGTCCTCT ATGAGCCCAG GTCTCCGTAG CTACTCAGAA 
GACACATCTT CCTACCCTAC TATTCGAAGG GTACAATCTC TGCATGTGCC TATGCAACCT 
CCGCCACCTC CTATCCGCAC TGTTCCAATT TCAAGAACAG AGGTTCCCCC AGATGATGAT 
CCAGCATACT GCCCAAGACC AGTTTATCAA TACAAACAAT GCCCCCAATT TAACTCTCAG 
TCAGATTACC ATGTTACCCA GCTGCAGCCT TACTTTGAAA ATGGACGAGT TCATTATCGC 
TACAGTCCCT ATTCAGGGGG CCCAAGTTAT TTTTCAGCAG ATAGCAATTT CTATGATATG 
GACCCCTACA GCACCTTACG TCTGCGACAA CTTCCTCTCT ACCCAAGCCG GGACTTTGCT 
TCTTACACCA CAAGATTGCA GCCCAAAAAC ACATATCGGT CACAAGGATT GCCCCCATAT 
CCCAGAGGAA CTCTACGGGA ACACAATTTT ATCAGTAGAG ATGTTCCTCC TGCACTACCA 
CCTGAACATC CACGACCTCT TCACATTTCT TGGGACATGG AAGACATGGA CAGATACAGA 
CTGCAATCCC TGCGACGAGA GAACAGGGCA CGTCAGAGAG CTAAAGGACC AGTAATGTCT 
CAGTATGATA ATGTTACACC AACCTTAGTG GAGGATGTAG CAGGCTTAGA TGTCATACAT 
CTCCGAAGCC GTTCTGATCC TGGTAAGACT CCTGGTCTTT TAAGTGTGGC TGAGAACAAA 
GACGTCCGTT ACCCAGGAAG GGCAGAAGGT GATGAGCATA CAACATATCC ACCTCCTGTT 
TTTGGAAATG GCCATCAAGA TAAGCCATCT CTTCCTCAAA AGCAAAGTGG ATCAAGCCGG 
GTTCGAATGC AACATGAAAT ATCTACTGAG CAGCGGACTC AACCTTCTCA AGATACTTTG 
CATAGGCAAC CCACAGAGGG ACGAAATGGT CCTCCAATTC CTCCTGTGGA CTACAGCTCT 
AAAGGAATGC CAAATCCCCC AGACCCTGCA TCATATCATT CCTCTGCAAA CAAATATAAC 
TTGACACAAC AGGAACCCAT GCGACTTATT CACAAAGAGC TTCGGCTCCC TGAGGATATT 
GAGCATTCAC ATACCAGGCC AGCTGACAAC CAACACCGTG ACTGTTACAG GGAGGAGCCA 
GCTCAGTTTC CCCTTACTGC ACCTCCTCCT AAACCAGAGA GGAGCCATAG CCTTCGAGGT 
CATAATCCAG ATGTTTTAGA AAGAGACCCT AGTATCTTCT ACCCCTACCA AACGCTTCAA 
GCCAAGAGAC ACAGCACAAT TAATGCAGTG TCACAATATG ATAATCTAGC TGATTACCAT 
TCTATACCCC ATCATCGAAC CACTAACCAG TCTACTCCAA ATGCCTTTAA CCTCCCACAT 
GGACGGACCT ATGCTACTGC CCTGGGCCAG GGTGCCTTTC TAGCTGCTGA ACTCGGCTTG 
CAAAGGCCTG AGACAAAAAT CCATGTGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916223.1
CDS	3018..8930
Translation	MEGGTDAAAFAAFPSIHAVPSTSGRMATGGKLDELERIMKSSLHLEEDDFVPELPRSIHPRERPDWEETISAMARGADIPVPGEIPTMPTEIPLRSCGSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGSIQLTLTEDQNEVSRNGCESKELVFLVQIACQGRSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLSELPRSDSVKDSPELVTQMLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINVPAIAAAHVIKRYNAQAPDELSFEVGDIVSVIDMPPKELTTWWRGKHGFQVGFFPSECVELINDKVPQSMTNSVPKPVSPAHGMRPASWSYFPPLSRKHGKLITFLRTFMKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSGAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_004916166.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916166.3

ORF Insert Sequence:

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGTCTCC 
CCTGCGCATG GAATGAGGCC GGCCTCCTGG AGCTACTTTC CCCCCTTGTC CCGGAAGCAC 
GGCAAGCTCA TCACATTTCT AAGAACCTTC ATGAAGTCCA GACCCAGCAA ACAGAAACTG 
AAGCAGAGAG GGATCCTGAG GGAGCGAGTG TTTGGTTGTG ACCTTGGGGA ACATCTGCTT 
AACTCTGGTC AGGATGTCCC CCAAGTCCTC AGGAGCTGCA CAGAGTTTAT AGAAAAACAT 
GGCATTGTTG ATGGAATCTA CCGTCTGTCT GGGATTGCAT CTAACATCCA GAAACTACGT 
CATGAATTTG ACTCTGAACA GATTCCAGAC CTGACTAAAG ATGTGTACAT CCAGGACATT 
CACTGTGTTG GCTCTCTCTG TAAACTCTAC TTCCGGGAAC TCCCAAACCC TCTGCTCACA 
TATCAGTTGT ATGAAAAGTT CTCTGATGCT GTGTCAGCGG CTACTGATGA AGAGAGACTG 
GTGAAAATTC ATGATGTTAT TCAACAGCTT CCCCCACCTC ATTACAGAAC ACTGGAGTTC 
CTTATGCGCC ACTTATCAAG ACTTGCGACA TACTGCTCTA TCACCAATAT GCACACTAAA 
AATTTGGCAA TTGTCTGGGC CCCTAACCTG CTCCGGTCAA AACAGATTGA GTCTGCATGT 
TTTAGCGGTA CAGCAGCTTT CATGGAAGTG CGCATACAGT CTGTGGTAGT TGAATTTATT 
CTAAACCATG TGGAGGTTCT CTTTAGTCCT AAACTTAGTT CTGTGATAAG GGATGGTGCT 
GGTCACACTT CTTTGTCTCG GCCCAAGTCA CTGTTGGTGT CATCTCCATC TACAAAACTA 
CTAAGCCTAG AGGAGGCTCA AGCAAGGACA CAGGCCCAAA TCAATTCCCC TGTTGTGGCT 
GACAGCAAGT ACATTGAGGT GGGAGAGGGA CCAGCTGCTT TGCAGGGCAG ATTCCACACT 
GTAATTGACT TCCCTTCTGA AAGGAAAAGA CCGCCAACCA AAATGAAAAA ATCTCCTGTT 
GGAAGCTGGC GTTCTTTTTT TAGCATAGGC AAATCATCAT CTTCAGCCTC CATGTCTAAG 
CGGAAACTTC AGCGTAATCC AAGTGAGCCT TTGGAAATGA AATCCATGGC ATTTGCAGGT 
GGAAGAGATA GCAGCACCAT GCGCTCAGCT AAAAGCGAGG AGTCTCTCAG TTCCTTGCAT 
GCAGCAGATG GAGAGTCGAA GCTATTTCGT CCACGTCGTC CTCGTTCCAG CAGCGATGCT 
CTGTCTGCTT CATATAATGG GGACCTTCTG GATAGCTGCA ACCGTTGCAA CTCCTATGAT 
AATCTGCCCC GAGACCAAGA CAGTGATGGA GATGAGGGTT TGATTCATGT GCCTGCTATG 
CTGTCAGGTC GCTCTCCTGA GGATGAAGAA CTCAGCCCAC CAGACATTGG TATGGCCAGT 
TTGGACTTTG ACCCAATGTC TTTTCAGTGC AGCCCTCCCC AGCTAGAATC TGAAGGACCA 
GACATTTTTT TTCAGCTTGA CATTTGTGCT AGCAATGGCA AGGATAAGGT GCAAAGTGGT 
GCACAAAACC TAGGCGGTGT CTCCAGCTCC GAACCTCACT CTCCTTTCCA AGAGAAAGTC 
GTTAGTCCAT TTCTATCGCC TGACCGCAGT TCTAGTACAG ATAAAGTATC CAAAACTACA 
TCATTTGCAG AAAAAGTTGT GCAGGCCTTG TCACCTAAAG TGGGGCGCAA AGCAGTTCGA 
TCTCCTCCAT TAACCATCTC AGAGCCTGTC ACCATCTCTC TCCCTAGTCG GGTATCGGAA 
ATGATTGGAA GTATGCAAGG CAATACTGCT GCTTCCCAGT CTTTAATTTG GTACAGAGAA 
AACAGTTTAA ATTCTAGAGA AACTGAAATG AATTATGTTT GTAAATCGGT GGATGCCAAC 
CTTCCATCCT TATTGCCAGC AACAGATCCA TGGTCTACAG TTACTCCTGT AATTACTCAA 
TGGAACCCAG ATGGATTACA GAGAGAAGAC AAAGCAGGAC CATTTACTCA TGGCTTTCCC 
CAGCCAAATA GTGACTTGGA ACAAGTAGAA AACTTCAACC TGGAACTTAG CACAACAAAT 
GTTGCTGCAG CATATCAGAA AGAAGATACA GAATTGTCGA GAGCAAATCA GAACAAATCC 
CACTCTTCTG TTTCTCCCCC TCAAGGAGCT TCTGCTTCAG AATCTCCACA GGAGCTAGCC 
CCTGTCAGTA GTGTTTCTAT CATTCCACCT CCACCTCCTC CAAAGAATCC TGCACGCATG 
CTGGCATTAG CACTGGCTGA ATCTGCTCAA CAGGCATCTG CTAATAAAAG ACCTCTTTAT 
ATGCAGTTCA TGGATCTACC CCCCCATCCT CCTCCACCAT CAGTACTAGA GGACAAACCA 
CTTCTTGAGT TATCTCCAAA AATGTCTCCA GAGCACCTCG TATCTGGTCC TGCAACACCA 
GCGACCAGTC CTCCAGTCAT ATCCAATAAA ACATCTCCAG CTACTCCACC AAGCACAACT 
TCATCATTTT CTATCACAAC AATTCATCAC AGTCCTGTGA AAAACCATAG TCCTCTGGCA 
GGACAGATGC CTACAGCAGT CACTCCTGGA CTCAGTCCTT CATCAAACCA GGTTCCTGTA 
TCCTTTCTAT CTGATGAAAT GCTACCTTCT GAAACAAATA AAATGAATTC TGTCATACTG 
AGTGAAGCAT TTACAACAGG AATTCCTGTT ACTCCACCAT CTGAAAAACC CAAGGAAATT 
TCTAGACCAC CACATCTTCA CCAGCGTTCT GAATCCTTTC CTTCACATCC TGCATATAGC 
AATGCAAAGC CCACTCCACC AGTTAGAACA ATGGATAGTC GACTGGCAAC TGCCATGCAT 
TCCAACTTCA ATGATGCCAT CACTGCTAAT AACTACCACT CCTTCCTGAA CACCATGATG 
CTTCCTTCTT CTCTAGAGGA TGTGCTACCC AGACACAACT ATTCTCATTC TTCTCATCTC 
AAGACAGAAA ATATAGATGA ACAGGGAGTT AATTACAGAC AACCTTATTT ATCCAACACC 
AAGCAAGAGG ACCCAGCTGA TGTGGGAGAT TTATACAGAC AGCAATATAT TTCTACTGGA 
AAGTCTGAGA ATGCTGAGAT AGGAAACACT TACCGGCACC CATATCCTTC CAATACTGAG 
CCAGAAAGTC TAAAGGAGCC ACTAGATCCT TTTCTGCATC ACAAGTCAGC AGTTGCTCCA 
AAGACATATA GAGCAGAAAC ACTTCTACCA CATATGCCTC CTCAAGTTTA TGGTTCAAGA 
TGTGATACTC CATCCTCAAC ATTTTATGGC ACCTACATAA ATCAGGCCAA ACACCCCCGC 
ACCAGGAACA GACCTGATTA TATGTCCTCT ATGAGCCCAG GTCTCCGTAG CTACTCAGAA 
GACACATCTT CCTACCCTAC TATTCGAAGG GTACAATCTC TGCATGTGCC TATGCAACCT 
CCGCCACCTC CTATCCGCAC TGTTCCAATT TCAAGAACAG AGGTTCCCCC AGATGATGAT 
CCAGCATACT GCCCAAGACC AGTTTATCAA TACAAACAAT GCCCCCAATT TAACTCTCAG 
TCAGATTACC ATGTTACCCA GCTGCAGCCT TACTTTGAAA ATGGACGAGT TCATTATCGC 
TACAGTCCCT ATTCAGGGGG CCCAAGTTAT TTTTCAGCAG ATAGCAATTT CTATGATATG 
GACCCCTACA GCACCTTACG TCTGCGACAA CTTCCTCTCT ACCCAAGCCG GGACTTTGCT 
TCTTACACCA CAAGATTGCA GCCCAAAAAC ACATATCGGT CACAAGGATT GCCCCCATAT 
CCCAGAGGAA CTCTACGGGA ACACAATTTT ATCAGTAGAG ATGTTCCTCC TGCACTACCA 
CCTGAACATC CACGACCTCT TCACATTTCT TGGGACATGG AAGACATGGA CAGATACAGA 
CTGCAATCCC TGCGACGAGA GAACAGGGCA CGTCAGAGAG CTAAAGGACC AGTAATGTCT 
CAGTATGATA ATGTTACACC AACCTTAGTG GAGGATGTAG CAGGCTTAGA TGTCATACAT 
CTCCGAAGCC GTTCTGATCC TGGTAAGACT CCTGGTCTTT TAAGTGTGGC TGAGAACAAA 
GACGTCCGTT ACCCAGGAAG GGCAGAAGGT GATGAGCATA CAACATATCC ACCTCCTGTT 
TTTGGAAATG GCCATCAAGA TAAGCCATCT CTTCCTCAAA AGCAAAGTGG ATCAAGCCGG 
GTTCGAATGC AACATGAAAT ATCTACTGAG CAGCGGACTC AACCTTCTCA AGATACTTTG 
CATAGGCAAC CCACAGAGGG ACGAAATGGT CCTCCAATTC CTCCTGTGGA CTACAGCTCT 
AAAGGAATGC CAAATCCCCC AGACCCTGCA TCATATCATT CCTCTGCAAA CAAATATAAC 
TTGACACAAC AGGAACCCAT GCGACTTATT CACAAAGAGC TTCGGCTCCC TGAGGATATT 
GAGCATTCAC ATACCAGGCC AGCTGACAAC CAACACCGTG ACTGTTACAG GGAGGAGCCA 
GCTCAGTTTC CCCTTACTGC ACCTCCTCCT AAACCAGAGA GGAGCCATAG CCTTCGAGGT 
CATAATCCAG ATGTTTTAGA AAGAGACCCT AGTATCTTCT ACCCCTACCA AACGCTTCAA 
GCCAAGAGAC ACAGCACAAT TAATGCAGTG TCACAATATG ATAATCTAGC TGATTACCAT 
TCTATACCCC ATCATCGAAC CACTAACCAG TCTACTCCAA ATGCCTTTAA CCTCCCACAT 
GGACGGACCT ATGCTACTGC CCTGGGCCAG GGTGCCTTTC TAGCTGCTGA ACTCGGCTTG 
CAAAGGCCTG AGACAAAAAT CCATGTGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_018095930.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951419.1 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X6, mRNA.

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CloneID	OXa13197
Clone ID Related Accession (Same CDS sequence)	XM_018095930.1
Accession Version	XM_018095930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCTT CACTGCACTT AGAGGAGGAT GATTTTGTAC CCGAGTTGCC AAGGAGTATC 
CATCCCCGGG AGAGGCCGGA CTGGGAGGAG ACTATTAGTG CCATGGCACG AGGGGCAGAC 
ATCCCAGTCC CAGGGGAAAT ACCGACGATG CCTACAGAAA TCCCTCTTCG CTCCTGTGGC 
AGTACTGCCA GCATGAAGGT GAAAAATGTC AAGAAGTTAT CCTTCACGAA AGGGCATTTT 
CCTAAACTTG CAGAATGTGC ACATTTCCAC TATGAAAATG TAGACTTTGG CAGCATACAG 
CTCACACTCA CAGAAGATCA AAATGAAGTG AGCAGAAATG GCTGTGAATC CAAGGAACTT 
GTTTTCCTAG TACAGATTGC TTGTCAGGGC CGTAGCTGGA TAGTTAAGCG GAGTTATGAA 
GATTTTCGGG TTCTGGATAA ACACCTTCAT CTGTGCATAT ATGATAGGCG CTTCTCTCAG 
CTCTCAGAGC TGCCCCGTTC CGACTCCGTA AAGGATAGTC CAGAGTTGGT CACACAGATG 
CTGATGGCTT ACCTATCGCG GCTGTCAGCC ATCGCAGGTA ACAAGATCAA TTGTGGGCCA 
GCTCTGACGT GGATGGAGAT CGATAATAAA GGCAATCATT TGCTTGTGCA CGAAGAGTCC 
TCCATAAATG TTCCAGCCAT CGCTGCTGCC CATGTCATAA AGCGATATAA TGCTCAGGCC 
CCAGATGAAC TCTCTTTTGA GGTGGGAGAC ATAGTATCTG TAATTGACAT GCCACCAAAA 
GAACTGACCA CGTGGTGGAG AGGAAAGCAT GGATTTCAAG TGGGTTTCTT TCCAAGTGAA 
TGTGTGGAAT TAATAAATGA TAAAGTCCCC CAATCAATGA CAAACTCTGT GCCTAAACCA 
GTCTCCCCTG CGCATGGAAT GAGGCCGGCC TCCTGGAGCT ACTTTCCCCC CTATTTGGAG 
ATGGATAGTT TAAGGCCTGG AACATCGTGC TGTTCTGAGG TATTAAGTCA CTCGCAGCTA 
AGCTCAGTGT CCCGGAAGCA CGGCAAGCTC ATCACATTTC TAAGAACCTT CATGAAGTCC 
AGACCCAGCA AACAGAAACT GAAGCAGAGA GGGATCCTGA GGGAGCGAGT GTTTGGTTGT 
GACCTTGGGG AACATCTGCT TAACTCTGGT CAGGATGTCC CCCAAGTCCT CAGGAGCTGC 
ACAGAGTTTA TAGAAAAACA TGGCATTGTT GATGGAATCT ACCGTCTGTC TGGGATTGCA 
TCTAACATCC AGAAACTACG TCATGAATTT GACTCTGAAC AGATTCCAGA CCTGACTAAA 
GATGTGTACA TCCAGGACAT TCACTGTGTT GGCTCTCTCT GTAAACTCTA CTTCCGGGAA 
CTCCCAAACC CTCTGCTCAC ATATCAGTTG TATGAAAAGT TCTCTGATGC TGTGTCAGCG 
GCTACTGATG AAGAGAGACT GGTGAAAATT CATGATGTTA TTCAACAGCT TCCCCCACCT 
CATTACAGAA CACTGGAGTT CCTTATGCGC CACTTATCAA GACTTGCGAC ATACTGCTCT 
ATCACCAATA TGCACACTAA AAATTTGGCA ATTGTCTGGG CCCCTAACCT GCTCCGGTCA 
AAACAGATTG AGTCTGCATG TTTTAGCGGT ACAGCAGCTT TCATGGAAGT GCGCATACAG 
TCTGTGGTAG TTGAATTTAT TCTAAACCAT GTGGAGGTTC TCTTTAGTCC TAAACTTAGT 
TCTGTGATAA GGGATGGTGC TGGTCACACT TCTTTGTCTC GGCCCAAGTC ACTGTTGGTG 
TCATCTCCAT CTACAAAACT ACTAAGCCTA GAGGAGGCTC AAGCAAGGAC ACAGGCCCAA 
ATCAATTCCC CTGTTGTGGC TGACAGCAAG TACATTGAGG TGGGAGAGGG ACCAGCTGCT 
TTGCAGGGCA GATTCCACAC TGTAATTGAC TTCCCTTCTG AAAGGAAAAG ACCGCCAACC 
AAAATGAAAA AATCTCCTGT TGGAAGCTGG CGTTCTTTTT TTAGCATAGG CAAATCATCA 
TCTTCAGCCT CCATGTCTAA GCGGAAACTT CAGCGTAATC CAAGTGAGCC TTTGGAAATG 
AAATCCATGG CATTTGCAGG TGGAAGAGAT AGCAGCACCA TGCGCTCAGC TAAAAGCGAG 
GAGTCTCTCA GTTCCTTGCA TGCAGCAGAT GGAGAGTCGA AGCTATTTCG TCCACGTCGT 
CCTCGTTCCA GCAGCGATGC TCTGTCTGCT TCATATAATG GGGACCTTCT GGATAGCTGC 
AACCGTTGCA ACTCCTATGA TAATCTGCCC CGAGACCAAG ACAGTGATGG AGATGAGGGT 
TTGATTCATG TGCCTGCTAT GCTGTCAGGT CGCTCTCCTG AGGATGAAGA ACTCAGCCCA 
CCAGACATTG GTATGGCCAG TTTGGACTTT GACCCAATGT CTTTTCAGTG CAGCCCTCCC 
CAGCTAGAAT CTGAAGGACC AGACATTTTT TTTCAGCTTG ACATTTGTGC TAGCAATGGC 
AAGGATAAGG TGCAAAGTGG TGCACAAAAC CTAGGCGGTG TCTCCAGCTC CGAACCTCAC 
TCTCCTTTCC AAGAGAAAGT CGTTAGTCCA TTTCTATCGC CTGACCGCAG TTCTAGTACA 
GATAAAGTAT CCAAAACTAC ATCATTTGCA GAAAAAGTTG TGCAGGCCTT GTCACCTAAA 
GTGGGGCGCA AAGCAGTTCG ATCTCCTCCA TTAACCATCT CAGAGCCTGT CACCATCTCT 
CTCCCTAGTC GGGTATCGGA AATGATTGGA AGTATGCAAG GCAATACTGC TGCTTCCCAG 
TCTTTAATTT GGTACAGAGA AAACAGTTTA AATTCTAGAG AAACTGAAAT GAATTATGTT 
TGTAAATCGG TGGATGCCAA CCTTCCATCC TTATTGCCAG CAACAGATCC ATGGTCTACA 
GTTACTCCTG TAATTACTCA ATGGAACCCA GATGGATTAC AGAGAGAAGA CAAAGCAGGA 
CCATTTACTC ATGGCTTTCC CCAGCCAAAT AGTGACTTGG AACAAGTAGA AAACTTCAAC 
CTGGAACTTA GCACAACAAA TGTTGCTGCA GCATATCAGA AAGAAGATAC AGAATTGTCG 
AGAGCAAATC AGAACAAATC CCACTCTTCT GTTTCTCCCC CTCAAGGAGC TTCTGCTTCA 
GAATCTCCAC AGGAGCTAGC CCCTGTCAGT AGTGTTTCTA TCATTCCACC TCCACCTCCT 
CCAAAGAATC CTGCACGCAT GCTGGCATTA GCACTGGCTG AATCTGCTCA ACAGGCATCT 
GCTAATAAAA GACCTCTTTA TATGCAGTTC ATGGATCTAC CCCCCCATCC TCCTCCACCA 
TCAGTACTAG AGGACAAACC ACTTCTTGAG TTATCTCCAA AAATGTCTCC AGAGCACCTC 
GTATCTGGTC CTGCAACACC AGCGACCAGT CCTCCAGTCA TATCCAATAA AACATCTCCA 
GCTACTCCAC CAAGCACAAC TTCATCATTT TCTATCACAA CAATTCATCA CAGTCCTGTG 
AAAAACCATA GTCCTCTGGC AGGACAGATG CCTACAGCAG TCACTCCTGG ACTCAGTCCT 
TCATCAAACC AGGTTCCTGT ATCCTTTCTA TCTGATGAAA TGCTACCTTC TGAAACAAAT 
AAAATGAATT CTGTCATACT GAGTGAAGCA TTTACAACAG GAATTCCTGT TACTCCACCA 
TCTGAAAAAC CCAAGGAAAT TTCTAGACCA CCACATCTTC ACCAGCGTTC TGAATCCTTT 
CCTTCACATC CTGCATATAG CAATGCAAAG CCCACTCCAC CAGTTAGAAC AATGGATAGT 
CGACTGGCAA CTGCCATGCA TTCCAACTTC AATGATGCCA TCACTGCTAA TAACTACCAC 
TCCTTCCTGA ACACCATGAT GCTTCCTTCT TCTCTAGAGG ATGTGCTACC CAGACACAAC 
TATTCTCATT CTTCTCATCT CAAGACAGAA AATATAGATG AACAGGGAGT TAATTACAGA 
CAACCTTATT TATCCAACAC CAAGCAAGAG GACCCAGCTG ATGTGGGAGA TTTATACAGA 
CAGCAATATA TTTCTACTGG AAAGTCTGAG AATGCTGAGA TAGGAAACAC TTACCGGCAC 
CCATATCCTT CCAATACTGA GCCAGAAAGT CTAAAGGAGC CACTAGATCC TTTTCTGCAT 
CACAAGTCAG CAGTTGCTCC AAAGACATAT AGAGCAGAAA CACTTCTACC ACATATGCCT 
CCTCAAGTTT ATGGTTCAAG ATGTGATACT CCATCCTCAA CATTTTATGG CACCTACATA 
AATCAGGCCA AACACCCCCG CACCAGGAAC AGACCTGATT ATATGTCCTC TATGAGCCCA 
GGTCTCCGTA GCTACTCAGA AGACACATCT TCCTACCCTA CTATTCGAAG GGTACAATCT 
CTGCATGTGC CTATGCAACC TCCGCCACCT CCTATCCGCA CTGTTCCAAT TTCAAGAACA 
GAGGTTCCCC CAGATGATGA TCCAGCATAC TGCCCAAGAC CAGTTTATCA ATACAAACAA 
TGCCCCCAAT TTAACTCTCA GTCAGATTAC CATGTTACCC AGCTGCAGCC TTACTTTGAA 
AATGGACGAG TTCATTATCG CTACAGTCCC TATTCAGGGG GCCCAAGTTA TTTTTCAGCA 
GATAGCAATT TCTATGATAT GGACCCCTAC AGCACCTTAC GTCTGCGACA ACTTCCTCTC 
TACCCAAGCC GGGACTTTGC TTCTTACACC ACAAGATTGC AGCCCAAAAA CACATATCGG 
TCACAAGGAT TGCCCCCATA TCCCAGAGGA ACTCTACGGG AACACAATTT TATCAGTAGA 
GATGTTCCTC CTGCACTACC ACCTGAACAT CCACGACCTC TTCACATTTC TTGGGACATG 
GAAGACATGG ACAGATACAG ACTGCAATCC CTGCGACGAG AGAACAGGGC ACGTCAGAGA 
GCTAAAGGAC CAGTAATGTC TCAGTATGAT AATGTTACAC CAACCTTAGT GGAGGATGTA 
GCAGGCTTAG ATGTCATACA TCTCCGAAGC CGTTCTGATC CTGGTAAGAC TCCTGGTCTT 
TTAAGTGTGG CTGAGAACAA AGACGTCCGT TACCCAGGAA GGGCAGAAGG TGATGAGCAT 
ACAACATATC CACCTCCTGT TTTTGGAAAT GGCCATCAAG ATAAGCCATC TCTTCCTCAA 
AAGCAAAGTG GATCAAGCCG GGTTCGAATG CAACATGAAA TATCTACTGA GCAGCGGACT 
CAACCTTCTC AAGATACTTT GCATAGGCAA CCCACAGAGG GACGAAATGG TCCTCCAATT 
CCTCCTGTGG ACTACAGCTC TAAAGGAATG CCAAATCCCC CAGACCCTGC ATCATATCAT 
TCCTCTGCAA ACAAATATAA CTTGACACAA CAGGAACCCA TGCGACTTAT TCACAAAGAG 
CTTCGGCTCC CTGAGGATAT TGAGCATTCA CATACCAGGC CAGCTGACAA CCAACACCGT 
GACTGTTACA GGGAGGAGCC AGCTCAGTTT CCCCTTACTG CACCTCCTCC TAAACCAGAG 
AGGAGCCATA GCCTTCGAGG TCATAATCCA GATGTTTTAG AAAGAGACCC TAGTATCTTC 
TACCCCTACC AAACGCTTCA AGCCAAGAGA CACAGCACAA TTAATGCAGT GTCACAATAT 
GATAATCTAG CTGATTACCA TTCTATACCC CATCATCGAA CCACTAACCA GTCTACTCCA 
AATGCCTTTA ACCTCCCACA TGGACGGACC TATGCTACTG CCCTGGGCCA GGGTGCCTTT 
CTAGCTGCTG AACTCGGCTT GCAAAGGCCT GAGACAAAAA TCCATGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951419.1
CDS	2843..8716
Translation	MKSSLHLEEDDFVPELPRSIHPRERPDWEETISAMARGADIPVPGEIPTMPTEIPLRSCGSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGSIQLTLTEDQNEVSRNGCESKELVFLVQIACQGRSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLSELPRSDSVKDSPELVTQMLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINVPAIAAAHVIKRYNAQAPDELSFEVGDIVSVIDMPPKELTTWWRGKHGFQVGFFPSECVELINDKVPQSMTNSVPKPVSPAHGMRPASWSYFPPYLEMDSLRPGTSCCSEVLSHSQLSSVSRKHGKLITFLRTFMKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSGAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_018095930.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095930.1

ORF Insert Sequence:

ATGAAGTCTT CACTGCACTT AGAGGAGGAT GATTTTGTAC CCGAGTTGCC AAGGAGTATC 
CATCCCCGGG AGAGGCCGGA CTGGGAGGAG ACTATTAGTG CCATGGCACG AGGGGCAGAC 
ATCCCAGTCC CAGGGGAAAT ACCGACGATG CCTACAGAAA TCCCTCTTCG CTCCTGTGGC 
AGTACTGCCA GCATGAAGGT GAAAAATGTC AAGAAGTTAT CCTTCACGAA AGGGCATTTT 
CCTAAACTTG CAGAATGTGC ACATTTCCAC TATGAAAATG TAGACTTTGG CAGCATACAG 
CTCACACTCA CAGAAGATCA AAATGAAGTG AGCAGAAATG GCTGTGAATC CAAGGAACTT 
GTTTTCCTAG TACAGATTGC TTGTCAGGGC CGTAGCTGGA TAGTTAAGCG GAGTTATGAA 
GATTTTCGGG TTCTGGATAA ACACCTTCAT CTGTGCATAT ATGATAGGCG CTTCTCTCAG 
CTCTCAGAGC TGCCCCGTTC CGACTCCGTA AAGGATAGTC CAGAGTTGGT CACACAGATG 
CTGATGGCTT ACCTATCGCG GCTGTCAGCC ATCGCAGGTA ACAAGATCAA TTGTGGGCCA 
GCTCTGACGT GGATGGAGAT CGATAATAAA GGCAATCATT TGCTTGTGCA CGAAGAGTCC 
TCCATAAATG TTCCAGCCAT CGCTGCTGCC CATGTCATAA AGCGATATAA TGCTCAGGCC 
CCAGATGAAC TCTCTTTTGA GGTGGGAGAC ATAGTATCTG TAATTGACAT GCCACCAAAA 
GAACTGACCA CGTGGTGGAG AGGAAAGCAT GGATTTCAAG TGGGTTTCTT TCCAAGTGAA 
TGTGTGGAAT TAATAAATGA TAAAGTCCCC CAATCAATGA CAAACTCTGT GCCTAAACCA 
GTCTCCCCTG CGCATGGAAT GAGGCCGGCC TCCTGGAGCT ACTTTCCCCC CTATTTGGAG 
ATGGATAGTT TAAGGCCTGG AACATCGTGC TGTTCTGAGG TATTAAGTCA CTCGCAGCTA 
AGCTCAGTGT CCCGGAAGCA CGGCAAGCTC ATCACATTTC TAAGAACCTT CATGAAGTCC 
AGACCCAGCA AACAGAAACT GAAGCAGAGA GGGATCCTGA GGGAGCGAGT GTTTGGTTGT 
GACCTTGGGG AACATCTGCT TAACTCTGGT CAGGATGTCC CCCAAGTCCT CAGGAGCTGC 
ACAGAGTTTA TAGAAAAACA TGGCATTGTT GATGGAATCT ACCGTCTGTC TGGGATTGCA 
TCTAACATCC AGAAACTACG TCATGAATTT GACTCTGAAC AGATTCCAGA CCTGACTAAA 
GATGTGTACA TCCAGGACAT TCACTGTGTT GGCTCTCTCT GTAAACTCTA CTTCCGGGAA 
CTCCCAAACC CTCTGCTCAC ATATCAGTTG TATGAAAAGT TCTCTGATGC TGTGTCAGCG 
GCTACTGATG AAGAGAGACT GGTGAAAATT CATGATGTTA TTCAACAGCT TCCCCCACCT 
CATTACAGAA CACTGGAGTT CCTTATGCGC CACTTATCAA GACTTGCGAC ATACTGCTCT 
ATCACCAATA TGCACACTAA AAATTTGGCA ATTGTCTGGG CCCCTAACCT GCTCCGGTCA 
AAACAGATTG AGTCTGCATG TTTTAGCGGT ACAGCAGCTT TCATGGAAGT GCGCATACAG 
TCTGTGGTAG TTGAATTTAT TCTAAACCAT GTGGAGGTTC TCTTTAGTCC TAAACTTAGT 
TCTGTGATAA GGGATGGTGC TGGTCACACT TCTTTGTCTC GGCCCAAGTC ACTGTTGGTG 
TCATCTCCAT CTACAAAACT ACTAAGCCTA GAGGAGGCTC AAGCAAGGAC ACAGGCCCAA 
ATCAATTCCC CTGTTGTGGC TGACAGCAAG TACATTGAGG TGGGAGAGGG ACCAGCTGCT 
TTGCAGGGCA GATTCCACAC TGTAATTGAC TTCCCTTCTG AAAGGAAAAG ACCGCCAACC 
AAAATGAAAA AATCTCCTGT TGGAAGCTGG CGTTCTTTTT TTAGCATAGG CAAATCATCA 
TCTTCAGCCT CCATGTCTAA GCGGAAACTT CAGCGTAATC CAAGTGAGCC TTTGGAAATG 
AAATCCATGG CATTTGCAGG TGGAAGAGAT AGCAGCACCA TGCGCTCAGC TAAAAGCGAG 
GAGTCTCTCA GTTCCTTGCA TGCAGCAGAT GGAGAGTCGA AGCTATTTCG TCCACGTCGT 
CCTCGTTCCA GCAGCGATGC TCTGTCTGCT TCATATAATG GGGACCTTCT GGATAGCTGC 
AACCGTTGCA ACTCCTATGA TAATCTGCCC CGAGACCAAG ACAGTGATGG AGATGAGGGT 
TTGATTCATG TGCCTGCTAT GCTGTCAGGT CGCTCTCCTG AGGATGAAGA ACTCAGCCCA 
CCAGACATTG GTATGGCCAG TTTGGACTTT GACCCAATGT CTTTTCAGTG CAGCCCTCCC 
CAGCTAGAAT CTGAAGGACC AGACATTTTT TTTCAGCTTG ACATTTGTGC TAGCAATGGC 
AAGGATAAGG TGCAAAGTGG TGCACAAAAC CTAGGCGGTG TCTCCAGCTC CGAACCTCAC 
TCTCCTTTCC AAGAGAAAGT CGTTAGTCCA TTTCTATCGC CTGACCGCAG TTCTAGTACA 
GATAAAGTAT CCAAAACTAC ATCATTTGCA GAAAAAGTTG TGCAGGCCTT GTCACCTAAA 
GTGGGGCGCA AAGCAGTTCG ATCTCCTCCA TTAACCATCT CAGAGCCTGT CACCATCTCT 
CTCCCTAGTC GGGTATCGGA AATGATTGGA AGTATGCAAG GCAATACTGC TGCTTCCCAG 
TCTTTAATTT GGTACAGAGA AAACAGTTTA AATTCTAGAG AAACTGAAAT GAATTATGTT 
TGTAAATCGG TGGATGCCAA CCTTCCATCC TTATTGCCAG CAACAGATCC ATGGTCTACA 
GTTACTCCTG TAATTACTCA ATGGAACCCA GATGGATTAC AGAGAGAAGA CAAAGCAGGA 
CCATTTACTC ATGGCTTTCC CCAGCCAAAT AGTGACTTGG AACAAGTAGA AAACTTCAAC 
CTGGAACTTA GCACAACAAA TGTTGCTGCA GCATATCAGA AAGAAGATAC AGAATTGTCG 
AGAGCAAATC AGAACAAATC CCACTCTTCT GTTTCTCCCC CTCAAGGAGC TTCTGCTTCA 
GAATCTCCAC AGGAGCTAGC CCCTGTCAGT AGTGTTTCTA TCATTCCACC TCCACCTCCT 
CCAAAGAATC CTGCACGCAT GCTGGCATTA GCACTGGCTG AATCTGCTCA ACAGGCATCT 
GCTAATAAAA GACCTCTTTA TATGCAGTTC ATGGATCTAC CCCCCCATCC TCCTCCACCA 
TCAGTACTAG AGGACAAACC ACTTCTTGAG TTATCTCCAA AAATGTCTCC AGAGCACCTC 
GTATCTGGTC CTGCAACACC AGCGACCAGT CCTCCAGTCA TATCCAATAA AACATCTCCA 
GCTACTCCAC CAAGCACAAC TTCATCATTT TCTATCACAA CAATTCATCA CAGTCCTGTG 
AAAAACCATA GTCCTCTGGC AGGACAGATG CCTACAGCAG TCACTCCTGG ACTCAGTCCT 
TCATCAAACC AGGTTCCTGT ATCCTTTCTA TCTGATGAAA TGCTACCTTC TGAAACAAAT 
AAAATGAATT CTGTCATACT GAGTGAAGCA TTTACAACAG GAATTCCTGT TACTCCACCA 
TCTGAAAAAC CCAAGGAAAT TTCTAGACCA CCACATCTTC ACCAGCGTTC TGAATCCTTT 
CCTTCACATC CTGCATATAG CAATGCAAAG CCCACTCCAC CAGTTAGAAC AATGGATAGT 
CGACTGGCAA CTGCCATGCA TTCCAACTTC AATGATGCCA TCACTGCTAA TAACTACCAC 
TCCTTCCTGA ACACCATGAT GCTTCCTTCT TCTCTAGAGG ATGTGCTACC CAGACACAAC 
TATTCTCATT CTTCTCATCT CAAGACAGAA AATATAGATG AACAGGGAGT TAATTACAGA 
CAACCTTATT TATCCAACAC CAAGCAAGAG GACCCAGCTG ATGTGGGAGA TTTATACAGA 
CAGCAATATA TTTCTACTGG AAAGTCTGAG AATGCTGAGA TAGGAAACAC TTACCGGCAC 
CCATATCCTT CCAATACTGA GCCAGAAAGT CTAAAGGAGC CACTAGATCC TTTTCTGCAT 
CACAAGTCAG CAGTTGCTCC AAAGACATAT AGAGCAGAAA CACTTCTACC ACATATGCCT 
CCTCAAGTTT ATGGTTCAAG ATGTGATACT CCATCCTCAA CATTTTATGG CACCTACATA 
AATCAGGCCA AACACCCCCG CACCAGGAAC AGACCTGATT ATATGTCCTC TATGAGCCCA 
GGTCTCCGTA GCTACTCAGA AGACACATCT TCCTACCCTA CTATTCGAAG GGTACAATCT 
CTGCATGTGC CTATGCAACC TCCGCCACCT CCTATCCGCA CTGTTCCAAT TTCAAGAACA 
GAGGTTCCCC CAGATGATGA TCCAGCATAC TGCCCAAGAC CAGTTTATCA ATACAAACAA 
TGCCCCCAAT TTAACTCTCA GTCAGATTAC CATGTTACCC AGCTGCAGCC TTACTTTGAA 
AATGGACGAG TTCATTATCG CTACAGTCCC TATTCAGGGG GCCCAAGTTA TTTTTCAGCA 
GATAGCAATT TCTATGATAT GGACCCCTAC AGCACCTTAC GTCTGCGACA ACTTCCTCTC 
TACCCAAGCC GGGACTTTGC TTCTTACACC ACAAGATTGC AGCCCAAAAA CACATATCGG 
TCACAAGGAT TGCCCCCATA TCCCAGAGGA ACTCTACGGG AACACAATTT TATCAGTAGA 
GATGTTCCTC CTGCACTACC ACCTGAACAT CCACGACCTC TTCACATTTC TTGGGACATG 
GAAGACATGG ACAGATACAG ACTGCAATCC CTGCGACGAG AGAACAGGGC ACGTCAGAGA 
GCTAAAGGAC CAGTAATGTC TCAGTATGAT AATGTTACAC CAACCTTAGT GGAGGATGTA 
GCAGGCTTAG ATGTCATACA TCTCCGAAGC CGTTCTGATC CTGGTAAGAC TCCTGGTCTT 
TTAAGTGTGG CTGAGAACAA AGACGTCCGT TACCCAGGAA GGGCAGAAGG TGATGAGCAT 
ACAACATATC CACCTCCTGT TTTTGGAAAT GGCCATCAAG ATAAGCCATC TCTTCCTCAA 
AAGCAAAGTG GATCAAGCCG GGTTCGAATG CAACATGAAA TATCTACTGA GCAGCGGACT 
CAACCTTCTC AAGATACTTT GCATAGGCAA CCCACAGAGG GACGAAATGG TCCTCCAATT 
CCTCCTGTGG ACTACAGCTC TAAAGGAATG CCAAATCCCC CAGACCCTGC ATCATATCAT 
TCCTCTGCAA ACAAATATAA CTTGACACAA CAGGAACCCA TGCGACTTAT TCACAAAGAG 
CTTCGGCTCC CTGAGGATAT TGAGCATTCA CATACCAGGC CAGCTGACAA CCAACACCGT 
GACTGTTACA GGGAGGAGCC AGCTCAGTTT CCCCTTACTG CACCTCCTCC TAAACCAGAG 
AGGAGCCATA GCCTTCGAGG TCATAATCCA GATGTTTTAG AAAGAGACCC TAGTATCTTC 
TACCCCTACC AAACGCTTCA AGCCAAGAGA CACAGCACAA TTAATGCAGT GTCACAATAT 
GATAATCTAG CTGATTACCA TTCTATACCC CATCATCGAA CCACTAACCA GTCTACTCCA 
AATGCCTTTA ACCTCCCACA TGGACGGACC TATGCTACTG CCCTGGGCCA GGGTGCCTTT 
CTAGCTGCTG AACTCGGCTT GCAAAGGCCT GAGACAAAAA TCCATGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_004916168.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916225.1 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X7, mRNA.

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CloneID	OXa13198
Clone ID Related Accession (Same CDS sequence)	XM_004916168.3
Accession Version	XM_004916168.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916168.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGTGTCC 
CGGAAGCACG GCAAGCTCAT CACATTTCTA AGAACCTTCA TGAAGTCCAG ACCCAGCAAA 
CAGAAACTGA AGCAGAGAGG GATCCTGAGG GAGCGAGTGT TTGGTTGTGA CCTTGGGGAA 
CATCTGCTTA ACTCTGGTCA GGATGTCCCC CAAGTCCTCA GGAGCTGCAC AGAGTTTATA 
GAAAAACATG GCATTGTTGA TGGAATCTAC CGTCTGTCTG GGATTGCATC TAACATCCAG 
AAACTACGTC ATGAATTTGA CTCTGAACAG ATTCCAGACC TGACTAAAGA TGTGTACATC 
CAGGACATTC ACTGTGTTGG CTCTCTCTGT AAACTCTACT TCCGGGAACT CCCAAACCCT 
CTGCTCACAT ATCAGTTGTA TGAAAAGTTC TCTGATGCTG TGTCAGCGGC TACTGATGAA 
GAGAGACTGG TGAAAATTCA TGATGTTATT CAACAGCTTC CCCCACCTCA TTACAGAACA 
CTGGAGTTCC TTATGCGCCA CTTATCAAGA CTTGCGACAT ACTGCTCTAT CACCAATATG 
CACACTAAAA ATTTGGCAAT TGTCTGGGCC CCTAACCTGC TCCGGTCAAA ACAGATTGAG 
TCTGCATGTT TTAGCGGTAC AGCAGCTTTC ATGGAAGTGC GCATACAGTC TGTGGTAGTT 
GAATTTATTC TAAACCATGT GGAGGTTCTC TTTAGTCCTA AACTTAGTTC TGTGATAAGG 
GATGGTGCTG GTCACACTTC TTTGTCTCGG CCCAAGTCAC TGTTGGTGTC ATCTCCATCT 
ACAAAACTAC TAAGCCTAGA GGAGGCTCAA GCAAGGACAC AGGCCCAAAT CAATTCCCCT 
GTTGTGGCTG ACAGCAAGTA CATTGAGGTG GGAGAGGGAC CAGCTGCTTT GCAGGGCAGA 
TTCCACACTG TAATTGACTT CCCTTCTGAA AGGAAAAGAC CGCCAACCAA AATGAAAAAA 
TCTCCTGTTG GAAGCTGGCG TTCTTTTTTT AGCATAGGCA AATCATCATC TTCAGCCTCC 
ATGTCTAAGC GGAAACTTCA GCGTAATCCA AGTGAGCCTT TGGAAATGAA ATCCATGGCA 
TTTGCAGGTG GAAGAGATAG CAGCACCATG CGCTCAGCTA AAAGCGAGGA GTCTCTCAGT 
TCCTTGCATG CAGCAGATGG AGAGTCGAAG CTATTTCGTC CACGTCGTCC TCGTTCCAGC 
AGCGATGCTC TGTCTGCTTC ATATAATGGG GACCTTCTGG ATAGCTGCAA CCGTTGCAAC 
TCCTATGATA ATCTGCCCCG AGACCAAGAC AGTGATGGAG ATGAGGGTTT GATTCATGTG 
CCTGCTATGC TGTCAGGTCG CTCTCCTGAG GATGAAGAAC TCAGCCCACC AGACATTGGT 
ATGGCCAGTT TGGACTTTGA CCCAATGTCT TTTCAGTGCA GCCCTCCCCA GCTAGAATCT 
GAAGGACCAG ACATTTTTTT TCAGCTTGAC ATTTGTGCTA GCAATGGCAA GGATAAGGTG 
CAAAGTGGTG CACAAAACCT AGGCGGTGTC TCCAGCTCCG AACCTCACTC TCCTTTCCAA 
GAGAAAGTCG TTAGTCCATT TCTATCGCCT GACCGCAGTT CTAGTACAGA TAAAGTATCC 
AAAACTACAT CATTTGCAGA AAAAGTTGTG CAGGCCTTGT CACCTAAAGT GGGGCGCAAA 
GCAGTTCGAT CTCCTCCATT AACCATCTCA GAGCCTGTCA CCATCTCTCT CCCTAGTCGG 
GTATCGGAAA TGATTGGAAG TATGCAAGGC AATACTGCTG CTTCCCAGTC TTTAATTTGG 
TACAGAGAAA ACAGTTTAAA TTCTAGAGAA ACTGAAATGA ATTATGTTTG TAAATCGGTG 
GATGCCAACC TTCCATCCTT ATTGCCAGCA ACAGATCCAT GGTCTACAGT TACTCCTGTA 
ATTACTCAAT GGAACCCAGA TGGATTACAG AGAGAAGACA AAGCAGGACC ATTTACTCAT 
GGCTTTCCCC AGCCAAATAG TGACTTGGAA CAAGTAGAAA ACTTCAACCT GGAACTTAGC 
ACAACAAATG TTGCTGCAGC ATATCAGAAA GAAGATACAG AATTGTCGAG AGCAAATCAG 
AACAAATCCC ACTCTTCTGT TTCTCCCCCT CAAGGAGCTT CTGCTTCAGA ATCTCCACAG 
GAGCTAGCCC CTGTCAGTAG TGTTTCTATC ATTCCACCTC CACCTCCTCC AAAGAATCCT 
GCACGCATGC TGGCATTAGC ACTGGCTGAA TCTGCTCAAC AGGCATCTGC TAATAAAAGA 
CCTCTTTATA TGCAGTTCAT GGATCTACCC CCCCATCCTC CTCCACCATC AGTACTAGAG 
GACAAACCAC TTCTTGAGTT ATCTCCAAAA ATGTCTCCAG AGCACCTCGT ATCTGGTCCT 
GCAACACCAG CGACCAGTCC TCCAGTCATA TCCAATAAAA CATCTCCAGC TACTCCACCA 
AGCACAACTT CATCATTTTC TATCACAACA ATTCATCACA GTCCTGTGAA AAACCATAGT 
CCTCTGGCAG GACAGATGCC TACAGCAGTC ACTCCTGGAC TCAGTCCTTC ATCAAACCAG 
GTTCCTGTAT CCTTTCTATC TGATGAAATG CTACCTTCTG AAACAAATAA AATGAATTCT 
GTCATACTGA GTGAAGCATT TACAACAGGA ATTCCTGTTA CTCCACCATC TGAAAAACCC 
AAGGAAATTT CTAGACCACC ACATCTTCAC CAGCGTTCTG AATCCTTTCC TTCACATCCT 
GCATATAGCA ATGCAAAGCC CACTCCACCA GTTAGAACAA TGGATAGTCG ACTGGCAACT 
GCCATGCATT CCAACTTCAA TGATGCCATC ACTGCTAATA ACTACCACTC CTTCCTGAAC 
ACCATGATGC TTCCTTCTTC TCTAGAGGAT GTGCTACCCA GACACAACTA TTCTCATTCT 
TCTCATCTCA AGACAGAAAA TATAGATGAA CAGGGAGTTA ATTACAGACA ACCTTATTTA 
TCCAACACCA AGCAAGAGGA CCCAGCTGAT GTGGGAGATT TATACAGACA GCAATATATT 
TCTACTGGAA AGTCTGAGAA TGCTGAGATA GGAAACACTT ACCGGCACCC ATATCCTTCC 
AATACTGAGC CAGAAAGTCT AAAGGAGCCA CTAGATCCTT TTCTGCATCA CAAGTCAGCA 
GTTGCTCCAA AGACATATAG AGCAGAAACA CTTCTACCAC ATATGCCTCC TCAAGTTTAT 
GGTTCAAGAT GTGATACTCC ATCCTCAACA TTTTATGGCA CCTACATAAA TCAGGCCAAA 
CACCCCCGCA CCAGGAACAG ACCTGATTAT ATGTCCTCTA TGAGCCCAGG TCTCCGTAGC 
TACTCAGAAG ACACATCTTC CTACCCTACT ATTCGAAGGG TACAATCTCT GCATGTGCCT 
ATGCAACCTC CGCCACCTCC TATCCGCACT GTTCCAATTT CAAGAACAGA GGTTCCCCCA 
GATGATGATC CAGCATACTG CCCAAGACCA GTTTATCAAT ACAAACAATG CCCCCAATTT 
AACTCTCAGT CAGATTACCA TGTTACCCAG CTGCAGCCTT ACTTTGAAAA TGGACGAGTT 
CATTATCGCT ACAGTCCCTA TTCAGGGGGC CCAAGTTATT TTTCAGCAGA TAGCAATTTC 
TATGATATGG ACCCCTACAG CACCTTACGT CTGCGACAAC TTCCTCTCTA CCCAAGCCGG 
GACTTTGCTT CTTACACCAC AAGATTGCAG CCCAAAAACA CATATCGGTC ACAAGGATTG 
CCCCCATATC CCAGAGGAAC TCTACGGGAA CACAATTTTA TCAGTAGAGA TGTTCCTCCT 
GCACTACCAC CTGAACATCC ACGACCTCTT CACATTTCTT GGGACATGGA AGACATGGAC 
AGATACAGAC TGCAATCCCT GCGACGAGAG AACAGGGCAC GTCAGAGAGC TAAAGGACCA 
GTAATGTCTC AGTATGATAA TGTTACACCA ACCTTAGTGG AGGATGTAGC AGGCTTAGAT 
GTCATACATC TCCGAAGCCG TTCTGATCCT GGTAAGACTC CTGGTCTTTT AAGTGTGGCT 
GAGAACAAAG ACGTCCGTTA CCCAGGAAGG GCAGAAGGTG ATGAGCATAC AACATATCCA 
CCTCCTGTTT TTGGAAATGG CCATCAAGAT AAGCCATCTC TTCCTCAAAA GCAAAGTGGA 
TCAAGCCGGG TTCGAATGCA ACATGAAATA TCTACTGAGC AGCGGACTCA ACCTTCTCAA 
GATACTTTGC ATAGGCAACC CACAGAGGGA CGAAATGGTC CTCCAATTCC TCCTGTGGAC 
TACAGCTCTA AAGGAATGCC AAATCCCCCA GACCCTGCAT CATATCATTC CTCTGCAAAC 
AAATATAACT TGACACAACA GGAACCCATG CGACTTATTC ACAAAGAGCT TCGGCTCCCT 
GAGGATATTG AGCATTCACA TACCAGGCCA GCTGACAACC AACACCGTGA CTGTTACAGG 
GAGGAGCCAG CTCAGTTTCC CCTTACTGCA CCTCCTCCTA AACCAGAGAG GAGCCATAGC 
CTTCGAGGTC ATAATCCAGA TGTTTTAGAA AGAGACCCTA GTATCTTCTA CCCCTACCAA 
ACGCTTCAAG CCAAGAGACA CAGCACAATT AATGCAGTGT CACAATATGA TAATCTAGCT 
GATTACCATT CTATACCCCA TCATCGAACC ACTAACCAGT CTACTCCAAA TGCCTTTAAC 
CTCCCACATG GACGGACCTA TGCTACTGCC CTGGGCCAGG GTGCCTTTCT AGCTGCTGAA 
CTCGGCTTGC AAAGGCCTGA GACAAAAATC CATGTGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916225.1
CDS	2863..8724
Translation	MEGGTDAAAFAAFPSIHAVPSTSGRMATGGKLDELERIMKSSLHLEEDDFVPELPRSIHPRERPDWEETISAMARGADIPVPGEIPTMPTEIPLRSCGSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGSIQLTLTEDQNEVSRNGCESKELVFLVQIACQGRSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLSELPRSDSVKDSPELVTQMLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINVPAIAAAHVIKRYNAQAPDELSFEVGDIVSVIDMPPKELTTWWRGKHGFQVGFFPSECVELINDKVPQSMTNSVPKPVSRKHGKLITFLRTFMKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSGAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_004916168.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916168.3

ORF Insert Sequence:

ATGGAGGGAG GGACCGACGC CGCTGCCTTC GCTGCTTTTC CCAGTATCCA CGCAGTTCCT 
TCGACTTCTG GCAGGATGGC AACGGGGGGC AAGCTGGATG AATTAGAAAG AATAATGAAG 
TCTTCACTGC ACTTAGAGGA GGATGATTTT GTACCCGAGT TGCCAAGGAG TATCCATCCC 
CGGGAGAGGC CGGACTGGGA GGAGACTATT AGTGCCATGG CACGAGGGGC AGACATCCCA 
GTCCCAGGGG AAATACCGAC GATGCCTACA GAAATCCCTC TTCGCTCCTG TGGCAGTACT 
GCCAGCATGA AGGTGAAAAA TGTCAAGAAG TTATCCTTCA CGAAAGGGCA TTTTCCTAAA 
CTTGCAGAAT GTGCACATTT CCACTATGAA AATGTAGACT TTGGCAGCAT ACAGCTCACA 
CTCACAGAAG ATCAAAATGA AGTGAGCAGA AATGGCTGTG AATCCAAGGA ACTTGTTTTC 
CTAGTACAGA TTGCTTGTCA GGGCCGTAGC TGGATAGTTA AGCGGAGTTA TGAAGATTTT 
CGGGTTCTGG ATAAACACCT TCATCTGTGC ATATATGATA GGCGCTTCTC TCAGCTCTCA 
GAGCTGCCCC GTTCCGACTC CGTAAAGGAT AGTCCAGAGT TGGTCACACA GATGCTGATG 
GCTTACCTAT CGCGGCTGTC AGCCATCGCA GGTAACAAGA TCAATTGTGG GCCAGCTCTG 
ACGTGGATGG AGATCGATAA TAAAGGCAAT CATTTGCTTG TGCACGAAGA GTCCTCCATA 
AATGTTCCAG CCATCGCTGC TGCCCATGTC ATAAAGCGAT ATAATGCTCA GGCCCCAGAT 
GAACTCTCTT TTGAGGTGGG AGACATAGTA TCTGTAATTG ACATGCCACC AAAAGAACTG 
ACCACGTGGT GGAGAGGAAA GCATGGATTT CAAGTGGGTT TCTTTCCAAG TGAATGTGTG 
GAATTAATAA ATGATAAAGT CCCCCAATCA ATGACAAACT CTGTGCCTAA ACCAGTGTCC 
CGGAAGCACG GCAAGCTCAT CACATTTCTA AGAACCTTCA TGAAGTCCAG ACCCAGCAAA 
CAGAAACTGA AGCAGAGAGG GATCCTGAGG GAGCGAGTGT TTGGTTGTGA CCTTGGGGAA 
CATCTGCTTA ACTCTGGTCA GGATGTCCCC CAAGTCCTCA GGAGCTGCAC AGAGTTTATA 
GAAAAACATG GCATTGTTGA TGGAATCTAC CGTCTGTCTG GGATTGCATC TAACATCCAG 
AAACTACGTC ATGAATTTGA CTCTGAACAG ATTCCAGACC TGACTAAAGA TGTGTACATC 
CAGGACATTC ACTGTGTTGG CTCTCTCTGT AAACTCTACT TCCGGGAACT CCCAAACCCT 
CTGCTCACAT ATCAGTTGTA TGAAAAGTTC TCTGATGCTG TGTCAGCGGC TACTGATGAA 
GAGAGACTGG TGAAAATTCA TGATGTTATT CAACAGCTTC CCCCACCTCA TTACAGAACA 
CTGGAGTTCC TTATGCGCCA CTTATCAAGA CTTGCGACAT ACTGCTCTAT CACCAATATG 
CACACTAAAA ATTTGGCAAT TGTCTGGGCC CCTAACCTGC TCCGGTCAAA ACAGATTGAG 
TCTGCATGTT TTAGCGGTAC AGCAGCTTTC ATGGAAGTGC GCATACAGTC TGTGGTAGTT 
GAATTTATTC TAAACCATGT GGAGGTTCTC TTTAGTCCTA AACTTAGTTC TGTGATAAGG 
GATGGTGCTG GTCACACTTC TTTGTCTCGG CCCAAGTCAC TGTTGGTGTC ATCTCCATCT 
ACAAAACTAC TAAGCCTAGA GGAGGCTCAA GCAAGGACAC AGGCCCAAAT CAATTCCCCT 
GTTGTGGCTG ACAGCAAGTA CATTGAGGTG GGAGAGGGAC CAGCTGCTTT GCAGGGCAGA 
TTCCACACTG TAATTGACTT CCCTTCTGAA AGGAAAAGAC CGCCAACCAA AATGAAAAAA 
TCTCCTGTTG GAAGCTGGCG TTCTTTTTTT AGCATAGGCA AATCATCATC TTCAGCCTCC 
ATGTCTAAGC GGAAACTTCA GCGTAATCCA AGTGAGCCTT TGGAAATGAA ATCCATGGCA 
TTTGCAGGTG GAAGAGATAG CAGCACCATG CGCTCAGCTA AAAGCGAGGA GTCTCTCAGT 
TCCTTGCATG CAGCAGATGG AGAGTCGAAG CTATTTCGTC CACGTCGTCC TCGTTCCAGC 
AGCGATGCTC TGTCTGCTTC ATATAATGGG GACCTTCTGG ATAGCTGCAA CCGTTGCAAC 
TCCTATGATA ATCTGCCCCG AGACCAAGAC AGTGATGGAG ATGAGGGTTT GATTCATGTG 
CCTGCTATGC TGTCAGGTCG CTCTCCTGAG GATGAAGAAC TCAGCCCACC AGACATTGGT 
ATGGCCAGTT TGGACTTTGA CCCAATGTCT TTTCAGTGCA GCCCTCCCCA GCTAGAATCT 
GAAGGACCAG ACATTTTTTT TCAGCTTGAC ATTTGTGCTA GCAATGGCAA GGATAAGGTG 
CAAAGTGGTG CACAAAACCT AGGCGGTGTC TCCAGCTCCG AACCTCACTC TCCTTTCCAA 
GAGAAAGTCG TTAGTCCATT TCTATCGCCT GACCGCAGTT CTAGTACAGA TAAAGTATCC 
AAAACTACAT CATTTGCAGA AAAAGTTGTG CAGGCCTTGT CACCTAAAGT GGGGCGCAAA 
GCAGTTCGAT CTCCTCCATT AACCATCTCA GAGCCTGTCA CCATCTCTCT CCCTAGTCGG 
GTATCGGAAA TGATTGGAAG TATGCAAGGC AATACTGCTG CTTCCCAGTC TTTAATTTGG 
TACAGAGAAA ACAGTTTAAA TTCTAGAGAA ACTGAAATGA ATTATGTTTG TAAATCGGTG 
GATGCCAACC TTCCATCCTT ATTGCCAGCA ACAGATCCAT GGTCTACAGT TACTCCTGTA 
ATTACTCAAT GGAACCCAGA TGGATTACAG AGAGAAGACA AAGCAGGACC ATTTACTCAT 
GGCTTTCCCC AGCCAAATAG TGACTTGGAA CAAGTAGAAA ACTTCAACCT GGAACTTAGC 
ACAACAAATG TTGCTGCAGC ATATCAGAAA GAAGATACAG AATTGTCGAG AGCAAATCAG 
AACAAATCCC ACTCTTCTGT TTCTCCCCCT CAAGGAGCTT CTGCTTCAGA ATCTCCACAG 
GAGCTAGCCC CTGTCAGTAG TGTTTCTATC ATTCCACCTC CACCTCCTCC AAAGAATCCT 
GCACGCATGC TGGCATTAGC ACTGGCTGAA TCTGCTCAAC AGGCATCTGC TAATAAAAGA 
CCTCTTTATA TGCAGTTCAT GGATCTACCC CCCCATCCTC CTCCACCATC AGTACTAGAG 
GACAAACCAC TTCTTGAGTT ATCTCCAAAA ATGTCTCCAG AGCACCTCGT ATCTGGTCCT 
GCAACACCAG CGACCAGTCC TCCAGTCATA TCCAATAAAA CATCTCCAGC TACTCCACCA 
AGCACAACTT CATCATTTTC TATCACAACA ATTCATCACA GTCCTGTGAA AAACCATAGT 
CCTCTGGCAG GACAGATGCC TACAGCAGTC ACTCCTGGAC TCAGTCCTTC ATCAAACCAG 
GTTCCTGTAT CCTTTCTATC TGATGAAATG CTACCTTCTG AAACAAATAA AATGAATTCT 
GTCATACTGA GTGAAGCATT TACAACAGGA ATTCCTGTTA CTCCACCATC TGAAAAACCC 
AAGGAAATTT CTAGACCACC ACATCTTCAC CAGCGTTCTG AATCCTTTCC TTCACATCCT 
GCATATAGCA ATGCAAAGCC CACTCCACCA GTTAGAACAA TGGATAGTCG ACTGGCAACT 
GCCATGCATT CCAACTTCAA TGATGCCATC ACTGCTAATA ACTACCACTC CTTCCTGAAC 
ACCATGATGC TTCCTTCTTC TCTAGAGGAT GTGCTACCCA GACACAACTA TTCTCATTCT 
TCTCATCTCA AGACAGAAAA TATAGATGAA CAGGGAGTTA ATTACAGACA ACCTTATTTA 
TCCAACACCA AGCAAGAGGA CCCAGCTGAT GTGGGAGATT TATACAGACA GCAATATATT 
TCTACTGGAA AGTCTGAGAA TGCTGAGATA GGAAACACTT ACCGGCACCC ATATCCTTCC 
AATACTGAGC CAGAAAGTCT AAAGGAGCCA CTAGATCCTT TTCTGCATCA CAAGTCAGCA 
GTTGCTCCAA AGACATATAG AGCAGAAACA CTTCTACCAC ATATGCCTCC TCAAGTTTAT 
GGTTCAAGAT GTGATACTCC ATCCTCAACA TTTTATGGCA CCTACATAAA TCAGGCCAAA 
CACCCCCGCA CCAGGAACAG ACCTGATTAT ATGTCCTCTA TGAGCCCAGG TCTCCGTAGC 
TACTCAGAAG ACACATCTTC CTACCCTACT ATTCGAAGGG TACAATCTCT GCATGTGCCT 
ATGCAACCTC CGCCACCTCC TATCCGCACT GTTCCAATTT CAAGAACAGA GGTTCCCCCA 
GATGATGATC CAGCATACTG CCCAAGACCA GTTTATCAAT ACAAACAATG CCCCCAATTT 
AACTCTCAGT CAGATTACCA TGTTACCCAG CTGCAGCCTT ACTTTGAAAA TGGACGAGTT 
CATTATCGCT ACAGTCCCTA TTCAGGGGGC CCAAGTTATT TTTCAGCAGA TAGCAATTTC 
TATGATATGG ACCCCTACAG CACCTTACGT CTGCGACAAC TTCCTCTCTA CCCAAGCCGG 
GACTTTGCTT CTTACACCAC AAGATTGCAG CCCAAAAACA CATATCGGTC ACAAGGATTG 
CCCCCATATC CCAGAGGAAC TCTACGGGAA CACAATTTTA TCAGTAGAGA TGTTCCTCCT 
GCACTACCAC CTGAACATCC ACGACCTCTT CACATTTCTT GGGACATGGA AGACATGGAC 
AGATACAGAC TGCAATCCCT GCGACGAGAG AACAGGGCAC GTCAGAGAGC TAAAGGACCA 
GTAATGTCTC AGTATGATAA TGTTACACCA ACCTTAGTGG AGGATGTAGC AGGCTTAGAT 
GTCATACATC TCCGAAGCCG TTCTGATCCT GGTAAGACTC CTGGTCTTTT AAGTGTGGCT 
GAGAACAAAG ACGTCCGTTA CCCAGGAAGG GCAGAAGGTG ATGAGCATAC AACATATCCA 
CCTCCTGTTT TTGGAAATGG CCATCAAGAT AAGCCATCTC TTCCTCAAAA GCAAAGTGGA 
TCAAGCCGGG TTCGAATGCA ACATGAAATA TCTACTGAGC AGCGGACTCA ACCTTCTCAA 
GATACTTTGC ATAGGCAACC CACAGAGGGA CGAAATGGTC CTCCAATTCC TCCTGTGGAC 
TACAGCTCTA AAGGAATGCC AAATCCCCCA GACCCTGCAT CATATCATTC CTCTGCAAAC 
AAATATAACT TGACACAACA GGAACCCATG CGACTTATTC ACAAAGAGCT TCGGCTCCCT 
GAGGATATTG AGCATTCACA TACCAGGCCA GCTGACAACC AACACCGTGA CTGTTACAGG 
GAGGAGCCAG CTCAGTTTCC CCTTACTGCA CCTCCTCCTA AACCAGAGAG GAGCCATAGC 
CTTCGAGGTC ATAATCCAGA TGTTTTAGAA AGAGACCCTA GTATCTTCTA CCCCTACCAA 
ACGCTTCAAG CCAAGAGACA CAGCACAATT AATGCAGTGT CACAATATGA TAATCTAGCT 
GATTACCATT CTATACCCCA TCATCGAACC ACTAACCAGT CTACTCCAAA TGCCTTTAAC 
CTCCCACATG GACGGACCTA TGCTACTGCC CTGGGCCAGG GTGCCTTTCT AGCTGCTGAA 
CTCGGCTTGC AAAGGCCTGA GACAAAAATC CATGTGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_004916169.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916226.1 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X10, mRNA.

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CloneID	OXa13199
Clone ID Related Accession (Same CDS sequence)	XM_004916169.3 , XM_004916170.3
Accession Version	XM_004916169.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916169.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCCA GACCCAGCAA ACAGAAACTG AAGCAGAGAG GGATCCTGAG GGAGCGAGTG 
TTTGGTTGTG ACCTTGGGGA ACATCTGCTT AACTCTGGTC AGGATGTCCC CCAAGTCCTC 
AGGAGCTGCA CAGAGTTTAT AGAAAAACAT GGCATTGTTG ATGGAATCTA CCGTCTGTCT 
GGGATTGCAT CTAACATCCA GAAACTACGT CATGAATTTG ACTCTGAACA GATTCCAGAC 
CTGACTAAAG ATGTGTACAT CCAGGACATT CACTGTGTTG GCTCTCTCTG TAAACTCTAC 
TTCCGGGAAC TCCCAAACCC TCTGCTCACA TATCAGTTGT ATGAAAAGTT CTCTGATGCT 
GTGTCAGCGG CTACTGATGA AGAGAGACTG GTGAAAATTC ATGATGTTAT TCAACAGCTT 
CCCCCACCTC ATTACAGAAC ACTGGAGTTC CTTATGCGCC ACTTATCAAG ACTTGCGACA 
TACTGCTCTA TCACCAATAT GCACACTAAA AATTTGGCAA TTGTCTGGGC CCCTAACCTG 
CTCCGGTCAA AACAGATTGA GTCTGCATGT TTTAGCGGTA CAGCAGCTTT CATGGAAGTG 
CGCATACAGT CTGTGGTAGT TGAATTTATT CTAAACCATG TGGAGGTTCT CTTTAGTCCT 
AAACTTAGTT CTGTGATAAG GGATGGTGCT GGTCACACTT CTTTGTCTCG GCCCAAGTCA 
CTGTTGGTGT CATCTCCATC TACAAAACTA CTAAGCCTAG AGGAGGCTCA AGCAAGGACA 
CAGGCCCAAA TCAATTCCCC TGTTGTGGCT GACAGCAAGT ACATTGAGGT GGGAGAGGGA 
CCAGCTGCTT TGCAGGGCAG ATTCCACACT GTAATTGACT TCCCTTCTGA AAGGAAAAGA 
CCGCCAACCA AAATGAAAAA ATCTCCTGTT GGAAGCTGGC GTTCTTTTTT TAGCATAGGC 
AAATCATCAT CTTCAGCCTC CATGTCTAAG CGGAAACTTC AGCGTAATCC AAGTGAGCCT 
TTGGAAATGA AATCCATGGC ATTTGCAGGT GGAAGAGATA GCAGCACCAT GCGCTCAGCT 
AAAAGCGAGG AGTCTCTCAG TTCCTTGCAT GCAGCAGATG GAGAGTCGAA GCTATTTCGT 
CCACGTCGTC CTCGTTCCAG CAGCGATGCT CTGTCTGCTT CATATAATGG GGACCTTCTG 
GATAGCTGCA ACCGTTGCAA CTCCTATGAT AATCTGCCCC GAGACCAAGA CAGTGATGGA 
GATGAGGGTT TGATTCATGT GCCTGCTATG CTGTCAGGTC GCTCTCCTGA GGATGAAGAA 
CTCAGCCCAC CAGACATTGG TATGGCCAGT TTGGACTTTG ACCCAATGTC TTTTCAGTGC 
AGCCCTCCCC AGCTAGAATC TGAAGGACCA GACATTTTTT TTCAGCTTGA CATTTGTGCT 
AGCAATGGCA AGGATAAGGT GCAAAGTGGT GCACAAAACC TAGGCGGTGT CTCCAGCTCC 
GAACCTCACT CTCCTTTCCA AGAGAAAGTC GTTAGTCCAT TTCTATCGCC TGACCGCAGT 
TCTAGTACAG ATAAAGTATC CAAAACTACA TCATTTGCAG AAAAAGTTGT GCAGGCCTTG 
TCACCTAAAG TGGGGCGCAA AGCAGTTCGA TCTCCTCCAT TAACCATCTC AGAGCCTGTC 
ACCATCTCTC TCCCTAGTCG GGTATCGGAA ATGATTGGAA GTATGCAAGG CAATACTGCT 
GCTTCCCAGT CTTTAATTTG GTACAGAGAA AACAGTTTAA ATTCTAGAGA AACTGAAATG 
AATTATGTTT GTAAATCGGT GGATGCCAAC CTTCCATCCT TATTGCCAGC AACAGATCCA 
TGGTCTACAG TTACTCCTGT AATTACTCAA TGGAACCCAG ATGGATTACA GAGAGAAGAC 
AAAGCAGGAC CATTTACTCA TGGCTTTCCC CAGCCAAATA GTGACTTGGA ACAAGTAGAA 
AACTTCAACC TGGAACTTAG CACAACAAAT GTTGCTGCAG CATATCAGAA AGAAGATACA 
GAATTGTCGA GAGCAAATCA GAACAAATCC CACTCTTCTG TTTCTCCCCC TCAAGGAGCT 
TCTGCTTCAG AATCTCCACA GGAGCTAGCC CCTGTCAGTA GTGTTTCTAT CATTCCACCT 
CCACCTCCTC CAAAGAATCC TGCACGCATG CTGGCATTAG CACTGGCTGA ATCTGCTCAA 
CAGGCATCTG CTAATAAAAG ACCTCTTTAT ATGCAGTTCA TGGATCTACC CCCCCATCCT 
CCTCCACCAT CAGTACTAGA GGACAAACCA CTTCTTGAGT TATCTCCAAA AATGTCTCCA 
GAGCACCTCG TATCTGGTCC TGCAACACCA GCGACCAGTC CTCCAGTCAT ATCCAATAAA 
ACATCTCCAG CTACTCCACC AAGCACAACT TCATCATTTT CTATCACAAC AATTCATCAC 
AGTCCTGTGA AAAACCATAG TCCTCTGGCA GGACAGATGC CTACAGCAGT CACTCCTGGA 
CTCAGTCCTT CATCAAACCA GGTTCCTGTA TCCTTTCTAT CTGATGAAAT GCTACCTTCT 
GAAACAAATA AAATGAATTC TGTCATACTG AGTGAAGCAT TTACAACAGG AATTCCTGTT 
ACTCCACCAT CTGAAAAACC CAAGGAAATT TCTAGACCAC CACATCTTCA CCAGCGTTCT 
GAATCCTTTC CTTCACATCC TGCATATAGC AATGCAAAGC CCACTCCACC AGTTAGAACA 
ATGGATAGTC GACTGGCAAC TGCCATGCAT TCCAACTTCA ATGATGCCAT CACTGCTAAT 
AACTACCACT CCTTCCTGAA CACCATGATG CTTCCTTCTT CTCTAGAGGA TGTGCTACCC 
AGACACAACT ATTCTCATTC TTCTCATCTC AAGACAGAAA ATATAGATGA ACAGGGAGTT 
AATTACAGAC AACCTTATTT ATCCAACACC AAGCAAGAGG ACCCAGCTGA TGTGGGAGAT 
TTATACAGAC AGCAATATAT TTCTACTGGA AAGTCTGAGA ATGCTGAGAT AGGAAACACT 
TACCGGCACC CATATCCTTC CAATACTGAG CCAGAAAGTC TAAAGGAGCC ACTAGATCCT 
TTTCTGCATC ACAAGTCAGC AGTTGCTCCA AAGACATATA GAGCAGAAAC ACTTCTACCA 
CATATGCCTC CTCAAGTTTA TGGTTCAAGA TGTGATACTC CATCCTCAAC ATTTTATGGC 
ACCTACATAA ATCAGGCCAA ACACCCCCGC ACCAGGAACA GACCTGATTA TATGTCCTCT 
ATGAGCCCAG GTCTCCGTAG CTACTCAGAA GACACATCTT CCTACCCTAC TATTCGAAGG 
GTACAATCTC TGCATGTGCC TATGCAACCT CCGCCACCTC CTATCCGCAC TGTTCCAATT 
TCAAGAACAG AGGTTCCCCC AGATGATGAT CCAGCATACT GCCCAAGACC AGTTTATCAA 
TACAAACAAT GCCCCCAATT TAACTCTCAG TCAGATTACC ATGTTACCCA GCTGCAGCCT 
TACTTTGAAA ATGGACGAGT TCATTATCGC TACAGTCCCT ATTCAGGGGG CCCAAGTTAT 
TTTTCAGCAG ATAGCAATTT CTATGATATG GACCCCTACA GCACCTTACG TCTGCGACAA 
CTTCCTCTCT ACCCAAGCCG GGACTTTGCT TCTTACACCA CAAGATTGCA GCCCAAAAAC 
ACATATCGGT CACAAGGATT GCCCCCATAT CCCAGAGGAA CTCTACGGGA ACACAATTTT 
ATCAGTAGAG ATGTTCCTCC TGCACTACCA CCTGAACATC CACGACCTCT TCACATTTCT 
TGGGACATGG AAGACATGGA CAGATACAGA CTGCAATCCC TGCGACGAGA GAACAGGGCA 
CGTCAGAGAG CTAAAGGACC AGTAATGTCT CAGTATGATA ATGTTACACC AACCTTAGTG 
GAGGATGTAG CAGGCTTAGA TGTCATACAT CTCCGAAGCC GTTCTGATCC TGGTAAGACT 
CCTGGTCTTT TAAGTGTGGC TGAGAACAAA GACGTCCGTT ACCCAGGAAG GGCAGAAGGT 
GATGAGCATA CAACATATCC ACCTCCTGTT TTTGGAAATG GCCATCAAGA TAAGCCATCT 
CTTCCTCAAA AGCAAAGTGG ATCAAGCCGG GTTCGAATGC AACATGAAAT ATCTACTGAG 
CAGCGGACTC AACCTTCTCA AGATACTTTG CATAGGCAAC CCACAGAGGG ACGAAATGGT 
CCTCCAATTC CTCCTGTGGA CTACAGCTCT AAAGGAATGC CAAATCCCCC AGACCCTGCA 
TCATATCATT CCTCTGCAAA CAAATATAAC TTGACACAAC AGGAACCCAT GCGACTTATT 
CACAAAGAGC TTCGGCTCCC TGAGGATATT GAGCATTCAC ATACCAGGCC AGCTGACAAC 
CAACACCGTG ACTGTTACAG GGAGGAGCCA GCTCAGTTTC CCCTTACTGC ACCTCCTCCT 
AAACCAGAGA GGAGCCATAG CCTTCGAGGT CATAATCCAG ATGTTTTAGA AAGAGACCCT 
AGTATCTTCT ACCCCTACCA AACGCTTCAA GCCAAGAGAC ACAGCACAAT TAATGCAGTG 
TCACAATATG ATAATCTAGC TGATTACCAT TCTATACCCC ATCATCGAAC CACTAACCAG 
TCTACTCCAA ATGCCTTTAA CCTCCCACAT GGACGGACCT ATGCTACTGC CCTGGGCCAG 
GGTGCCTTTC TAGCTGCTGA ACTCGGCTTG CAAAGGCCTG AGACAAAAAT CCATGTGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916226.1
CDS	372..5174
Translation	MKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSGAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_004916169.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916169.3

ORF Insert Sequence:

ATGAAGTCCA GACCCAGCAA ACAGAAACTG AAGCAGAGAG GGATCCTGAG GGAGCGAGTG 
TTTGGTTGTG ACCTTGGGGA ACATCTGCTT AACTCTGGTC AGGATGTCCC CCAAGTCCTC 
AGGAGCTGCA CAGAGTTTAT AGAAAAACAT GGCATTGTTG ATGGAATCTA CCGTCTGTCT 
GGGATTGCAT CTAACATCCA GAAACTACGT CATGAATTTG ACTCTGAACA GATTCCAGAC 
CTGACTAAAG ATGTGTACAT CCAGGACATT CACTGTGTTG GCTCTCTCTG TAAACTCTAC 
TTCCGGGAAC TCCCAAACCC TCTGCTCACA TATCAGTTGT ATGAAAAGTT CTCTGATGCT 
GTGTCAGCGG CTACTGATGA AGAGAGACTG GTGAAAATTC ATGATGTTAT TCAACAGCTT 
CCCCCACCTC ATTACAGAAC ACTGGAGTTC CTTATGCGCC ACTTATCAAG ACTTGCGACA 
TACTGCTCTA TCACCAATAT GCACACTAAA AATTTGGCAA TTGTCTGGGC CCCTAACCTG 
CTCCGGTCAA AACAGATTGA GTCTGCATGT TTTAGCGGTA CAGCAGCTTT CATGGAAGTG 
CGCATACAGT CTGTGGTAGT TGAATTTATT CTAAACCATG TGGAGGTTCT CTTTAGTCCT 
AAACTTAGTT CTGTGATAAG GGATGGTGCT GGTCACACTT CTTTGTCTCG GCCCAAGTCA 
CTGTTGGTGT CATCTCCATC TACAAAACTA CTAAGCCTAG AGGAGGCTCA AGCAAGGACA 
CAGGCCCAAA TCAATTCCCC TGTTGTGGCT GACAGCAAGT ACATTGAGGT GGGAGAGGGA 
CCAGCTGCTT TGCAGGGCAG ATTCCACACT GTAATTGACT TCCCTTCTGA AAGGAAAAGA 
CCGCCAACCA AAATGAAAAA ATCTCCTGTT GGAAGCTGGC GTTCTTTTTT TAGCATAGGC 
AAATCATCAT CTTCAGCCTC CATGTCTAAG CGGAAACTTC AGCGTAATCC AAGTGAGCCT 
TTGGAAATGA AATCCATGGC ATTTGCAGGT GGAAGAGATA GCAGCACCAT GCGCTCAGCT 
AAAAGCGAGG AGTCTCTCAG TTCCTTGCAT GCAGCAGATG GAGAGTCGAA GCTATTTCGT 
CCACGTCGTC CTCGTTCCAG CAGCGATGCT CTGTCTGCTT CATATAATGG GGACCTTCTG 
GATAGCTGCA ACCGTTGCAA CTCCTATGAT AATCTGCCCC GAGACCAAGA CAGTGATGGA 
GATGAGGGTT TGATTCATGT GCCTGCTATG CTGTCAGGTC GCTCTCCTGA GGATGAAGAA 
CTCAGCCCAC CAGACATTGG TATGGCCAGT TTGGACTTTG ACCCAATGTC TTTTCAGTGC 
AGCCCTCCCC AGCTAGAATC TGAAGGACCA GACATTTTTT TTCAGCTTGA CATTTGTGCT 
AGCAATGGCA AGGATAAGGT GCAAAGTGGT GCACAAAACC TAGGCGGTGT CTCCAGCTCC 
GAACCTCACT CTCCTTTCCA AGAGAAAGTC GTTAGTCCAT TTCTATCGCC TGACCGCAGT 
TCTAGTACAG ATAAAGTATC CAAAACTACA TCATTTGCAG AAAAAGTTGT GCAGGCCTTG 
TCACCTAAAG TGGGGCGCAA AGCAGTTCGA TCTCCTCCAT TAACCATCTC AGAGCCTGTC 
ACCATCTCTC TCCCTAGTCG GGTATCGGAA ATGATTGGAA GTATGCAAGG CAATACTGCT 
GCTTCCCAGT CTTTAATTTG GTACAGAGAA AACAGTTTAA ATTCTAGAGA AACTGAAATG 
AATTATGTTT GTAAATCGGT GGATGCCAAC CTTCCATCCT TATTGCCAGC AACAGATCCA 
TGGTCTACAG TTACTCCTGT AATTACTCAA TGGAACCCAG ATGGATTACA GAGAGAAGAC 
AAAGCAGGAC CATTTACTCA TGGCTTTCCC CAGCCAAATA GTGACTTGGA ACAAGTAGAA 
AACTTCAACC TGGAACTTAG CACAACAAAT GTTGCTGCAG CATATCAGAA AGAAGATACA 
GAATTGTCGA GAGCAAATCA GAACAAATCC CACTCTTCTG TTTCTCCCCC TCAAGGAGCT 
TCTGCTTCAG AATCTCCACA GGAGCTAGCC CCTGTCAGTA GTGTTTCTAT CATTCCACCT 
CCACCTCCTC CAAAGAATCC TGCACGCATG CTGGCATTAG CACTGGCTGA ATCTGCTCAA 
CAGGCATCTG CTAATAAAAG ACCTCTTTAT ATGCAGTTCA TGGATCTACC CCCCCATCCT 
CCTCCACCAT CAGTACTAGA GGACAAACCA CTTCTTGAGT TATCTCCAAA AATGTCTCCA 
GAGCACCTCG TATCTGGTCC TGCAACACCA GCGACCAGTC CTCCAGTCAT ATCCAATAAA 
ACATCTCCAG CTACTCCACC AAGCACAACT TCATCATTTT CTATCACAAC AATTCATCAC 
AGTCCTGTGA AAAACCATAG TCCTCTGGCA GGACAGATGC CTACAGCAGT CACTCCTGGA 
CTCAGTCCTT CATCAAACCA GGTTCCTGTA TCCTTTCTAT CTGATGAAAT GCTACCTTCT 
GAAACAAATA AAATGAATTC TGTCATACTG AGTGAAGCAT TTACAACAGG AATTCCTGTT 
ACTCCACCAT CTGAAAAACC CAAGGAAATT TCTAGACCAC CACATCTTCA CCAGCGTTCT 
GAATCCTTTC CTTCACATCC TGCATATAGC AATGCAAAGC CCACTCCACC AGTTAGAACA 
ATGGATAGTC GACTGGCAAC TGCCATGCAT TCCAACTTCA ATGATGCCAT CACTGCTAAT 
AACTACCACT CCTTCCTGAA CACCATGATG CTTCCTTCTT CTCTAGAGGA TGTGCTACCC 
AGACACAACT ATTCTCATTC TTCTCATCTC AAGACAGAAA ATATAGATGA ACAGGGAGTT 
AATTACAGAC AACCTTATTT ATCCAACACC AAGCAAGAGG ACCCAGCTGA TGTGGGAGAT 
TTATACAGAC AGCAATATAT TTCTACTGGA AAGTCTGAGA ATGCTGAGAT AGGAAACACT 
TACCGGCACC CATATCCTTC CAATACTGAG CCAGAAAGTC TAAAGGAGCC ACTAGATCCT 
TTTCTGCATC ACAAGTCAGC AGTTGCTCCA AAGACATATA GAGCAGAAAC ACTTCTACCA 
CATATGCCTC CTCAAGTTTA TGGTTCAAGA TGTGATACTC CATCCTCAAC ATTTTATGGC 
ACCTACATAA ATCAGGCCAA ACACCCCCGC ACCAGGAACA GACCTGATTA TATGTCCTCT 
ATGAGCCCAG GTCTCCGTAG CTACTCAGAA GACACATCTT CCTACCCTAC TATTCGAAGG 
GTACAATCTC TGCATGTGCC TATGCAACCT CCGCCACCTC CTATCCGCAC TGTTCCAATT 
TCAAGAACAG AGGTTCCCCC AGATGATGAT CCAGCATACT GCCCAAGACC AGTTTATCAA 
TACAAACAAT GCCCCCAATT TAACTCTCAG TCAGATTACC ATGTTACCCA GCTGCAGCCT 
TACTTTGAAA ATGGACGAGT TCATTATCGC TACAGTCCCT ATTCAGGGGG CCCAAGTTAT 
TTTTCAGCAG ATAGCAATTT CTATGATATG GACCCCTACA GCACCTTACG TCTGCGACAA 
CTTCCTCTCT ACCCAAGCCG GGACTTTGCT TCTTACACCA CAAGATTGCA GCCCAAAAAC 
ACATATCGGT CACAAGGATT GCCCCCATAT CCCAGAGGAA CTCTACGGGA ACACAATTTT 
ATCAGTAGAG ATGTTCCTCC TGCACTACCA CCTGAACATC CACGACCTCT TCACATTTCT 
TGGGACATGG AAGACATGGA CAGATACAGA CTGCAATCCC TGCGACGAGA GAACAGGGCA 
CGTCAGAGAG CTAAAGGACC AGTAATGTCT CAGTATGATA ATGTTACACC AACCTTAGTG 
GAGGATGTAG CAGGCTTAGA TGTCATACAT CTCCGAAGCC GTTCTGATCC TGGTAAGACT 
CCTGGTCTTT TAAGTGTGGC TGAGAACAAA GACGTCCGTT ACCCAGGAAG GGCAGAAGGT 
GATGAGCATA CAACATATCC ACCTCCTGTT TTTGGAAATG GCCATCAAGA TAAGCCATCT 
CTTCCTCAAA AGCAAAGTGG ATCAAGCCGG GTTCGAATGC AACATGAAAT ATCTACTGAG 
CAGCGGACTC AACCTTCTCA AGATACTTTG CATAGGCAAC CCACAGAGGG ACGAAATGGT 
CCTCCAATTC CTCCTGTGGA CTACAGCTCT AAAGGAATGC CAAATCCCCC AGACCCTGCA 
TCATATCATT CCTCTGCAAA CAAATATAAC TTGACACAAC AGGAACCCAT GCGACTTATT 
CACAAAGAGC TTCGGCTCCC TGAGGATATT GAGCATTCAC ATACCAGGCC AGCTGACAAC 
CAACACCGTG ACTGTTACAG GGAGGAGCCA GCTCAGTTTC CCCTTACTGC ACCTCCTCCT 
AAACCAGAGA GGAGCCATAG CCTTCGAGGT CATAATCCAG ATGTTTTAGA AAGAGACCCT 
AGTATCTTCT ACCCCTACCA AACGCTTCAA GCCAAGAGAC ACAGCACAAT TAATGCAGTG 
TCACAATATG ATAATCTAGC TGATTACCAT TCTATACCCC ATCATCGAAC CACTAACCAG 
TCTACTCCAA ATGCCTTTAA CCTCCCACAT GGACGGACCT ATGCTACTGC CCTGGGCCAG 
GGTGCCTTTC TAGCTGCTGA ACTCGGCTTG CAAAGGCCTG AGACAAAAAT CCATGTGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_004916170.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916227.1 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X11, mRNA.

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CloneID	OXa13199
Clone ID Related Accession (Same CDS sequence)	XM_004916169.3 , XM_004916170.3
Accession Version	XM_004916170.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916170.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCCA GACCCAGCAA ACAGAAACTG AAGCAGAGAG GGATCCTGAG GGAGCGAGTG 
TTTGGTTGTG ACCTTGGGGA ACATCTGCTT AACTCTGGTC AGGATGTCCC CCAAGTCCTC 
AGGAGCTGCA CAGAGTTTAT AGAAAAACAT GGCATTGTTG ATGGAATCTA CCGTCTGTCT 
GGGATTGCAT CTAACATCCA GAAACTACGT CATGAATTTG ACTCTGAACA GATTCCAGAC 
CTGACTAAAG ATGTGTACAT CCAGGACATT CACTGTGTTG GCTCTCTCTG TAAACTCTAC 
TTCCGGGAAC TCCCAAACCC TCTGCTCACA TATCAGTTGT ATGAAAAGTT CTCTGATGCT 
GTGTCAGCGG CTACTGATGA AGAGAGACTG GTGAAAATTC ATGATGTTAT TCAACAGCTT 
CCCCCACCTC ATTACAGAAC ACTGGAGTTC CTTATGCGCC ACTTATCAAG ACTTGCGACA 
TACTGCTCTA TCACCAATAT GCACACTAAA AATTTGGCAA TTGTCTGGGC CCCTAACCTG 
CTCCGGTCAA AACAGATTGA GTCTGCATGT TTTAGCGGTA CAGCAGCTTT CATGGAAGTG 
CGCATACAGT CTGTGGTAGT TGAATTTATT CTAAACCATG TGGAGGTTCT CTTTAGTCCT 
AAACTTAGTT CTGTGATAAG GGATGGTGCT GGTCACACTT CTTTGTCTCG GCCCAAGTCA 
CTGTTGGTGT CATCTCCATC TACAAAACTA CTAAGCCTAG AGGAGGCTCA AGCAAGGACA 
CAGGCCCAAA TCAATTCCCC TGTTGTGGCT GACAGCAAGT ACATTGAGGT GGGAGAGGGA 
CCAGCTGCTT TGCAGGGCAG ATTCCACACT GTAATTGACT TCCCTTCTGA AAGGAAAAGA 
CCGCCAACCA AAATGAAAAA ATCTCCTGTT GGAAGCTGGC GTTCTTTTTT TAGCATAGGC 
AAATCATCAT CTTCAGCCTC CATGTCTAAG CGGAAACTTC AGCGTAATCC AAGTGAGCCT 
TTGGAAATGA AATCCATGGC ATTTGCAGGT GGAAGAGATA GCAGCACCAT GCGCTCAGCT 
AAAAGCGAGG AGTCTCTCAG TTCCTTGCAT GCAGCAGATG GAGAGTCGAA GCTATTTCGT 
CCACGTCGTC CTCGTTCCAG CAGCGATGCT CTGTCTGCTT CATATAATGG GGACCTTCTG 
GATAGCTGCA ACCGTTGCAA CTCCTATGAT AATCTGCCCC GAGACCAAGA CAGTGATGGA 
GATGAGGGTT TGATTCATGT GCCTGCTATG CTGTCAGGTC GCTCTCCTGA GGATGAAGAA 
CTCAGCCCAC CAGACATTGG TATGGCCAGT TTGGACTTTG ACCCAATGTC TTTTCAGTGC 
AGCCCTCCCC AGCTAGAATC TGAAGGACCA GACATTTTTT TTCAGCTTGA CATTTGTGCT 
AGCAATGGCA AGGATAAGGT GCAAAGTGGT GCACAAAACC TAGGCGGTGT CTCCAGCTCC 
GAACCTCACT CTCCTTTCCA AGAGAAAGTC GTTAGTCCAT TTCTATCGCC TGACCGCAGT 
TCTAGTACAG ATAAAGTATC CAAAACTACA TCATTTGCAG AAAAAGTTGT GCAGGCCTTG 
TCACCTAAAG TGGGGCGCAA AGCAGTTCGA TCTCCTCCAT TAACCATCTC AGAGCCTGTC 
ACCATCTCTC TCCCTAGTCG GGTATCGGAA ATGATTGGAA GTATGCAAGG CAATACTGCT 
GCTTCCCAGT CTTTAATTTG GTACAGAGAA AACAGTTTAA ATTCTAGAGA AACTGAAATG 
AATTATGTTT GTAAATCGGT GGATGCCAAC CTTCCATCCT TATTGCCAGC AACAGATCCA 
TGGTCTACAG TTACTCCTGT AATTACTCAA TGGAACCCAG ATGGATTACA GAGAGAAGAC 
AAAGCAGGAC CATTTACTCA TGGCTTTCCC CAGCCAAATA GTGACTTGGA ACAAGTAGAA 
AACTTCAACC TGGAACTTAG CACAACAAAT GTTGCTGCAG CATATCAGAA AGAAGATACA 
GAATTGTCGA GAGCAAATCA GAACAAATCC CACTCTTCTG TTTCTCCCCC TCAAGGAGCT 
TCTGCTTCAG AATCTCCACA GGAGCTAGCC CCTGTCAGTA GTGTTTCTAT CATTCCACCT 
CCACCTCCTC CAAAGAATCC TGCACGCATG CTGGCATTAG CACTGGCTGA ATCTGCTCAA 
CAGGCATCTG CTAATAAAAG ACCTCTTTAT ATGCAGTTCA TGGATCTACC CCCCCATCCT 
CCTCCACCAT CAGTACTAGA GGACAAACCA CTTCTTGAGT TATCTCCAAA AATGTCTCCA 
GAGCACCTCG TATCTGGTCC TGCAACACCA GCGACCAGTC CTCCAGTCAT ATCCAATAAA 
ACATCTCCAG CTACTCCACC AAGCACAACT TCATCATTTT CTATCACAAC AATTCATCAC 
AGTCCTGTGA AAAACCATAG TCCTCTGGCA GGACAGATGC CTACAGCAGT CACTCCTGGA 
CTCAGTCCTT CATCAAACCA GGTTCCTGTA TCCTTTCTAT CTGATGAAAT GCTACCTTCT 
GAAACAAATA AAATGAATTC TGTCATACTG AGTGAAGCAT TTACAACAGG AATTCCTGTT 
ACTCCACCAT CTGAAAAACC CAAGGAAATT TCTAGACCAC CACATCTTCA CCAGCGTTCT 
GAATCCTTTC CTTCACATCC TGCATATAGC AATGCAAAGC CCACTCCACC AGTTAGAACA 
ATGGATAGTC GACTGGCAAC TGCCATGCAT TCCAACTTCA ATGATGCCAT CACTGCTAAT 
AACTACCACT CCTTCCTGAA CACCATGATG CTTCCTTCTT CTCTAGAGGA TGTGCTACCC 
AGACACAACT ATTCTCATTC TTCTCATCTC AAGACAGAAA ATATAGATGA ACAGGGAGTT 
AATTACAGAC AACCTTATTT ATCCAACACC AAGCAAGAGG ACCCAGCTGA TGTGGGAGAT 
TTATACAGAC AGCAATATAT TTCTACTGGA AAGTCTGAGA ATGCTGAGAT AGGAAACACT 
TACCGGCACC CATATCCTTC CAATACTGAG CCAGAAAGTC TAAAGGAGCC ACTAGATCCT 
TTTCTGCATC ACAAGTCAGC AGTTGCTCCA AAGACATATA GAGCAGAAAC ACTTCTACCA 
CATATGCCTC CTCAAGTTTA TGGTTCAAGA TGTGATACTC CATCCTCAAC ATTTTATGGC 
ACCTACATAA ATCAGGCCAA ACACCCCCGC ACCAGGAACA GACCTGATTA TATGTCCTCT 
ATGAGCCCAG GTCTCCGTAG CTACTCAGAA GACACATCTT CCTACCCTAC TATTCGAAGG 
GTACAATCTC TGCATGTGCC TATGCAACCT CCGCCACCTC CTATCCGCAC TGTTCCAATT 
TCAAGAACAG AGGTTCCCCC AGATGATGAT CCAGCATACT GCCCAAGACC AGTTTATCAA 
TACAAACAAT GCCCCCAATT TAACTCTCAG TCAGATTACC ATGTTACCCA GCTGCAGCCT 
TACTTTGAAA ATGGACGAGT TCATTATCGC TACAGTCCCT ATTCAGGGGG CCCAAGTTAT 
TTTTCAGCAG ATAGCAATTT CTATGATATG GACCCCTACA GCACCTTACG TCTGCGACAA 
CTTCCTCTCT ACCCAAGCCG GGACTTTGCT TCTTACACCA CAAGATTGCA GCCCAAAAAC 
ACATATCGGT CACAAGGATT GCCCCCATAT CCCAGAGGAA CTCTACGGGA ACACAATTTT 
ATCAGTAGAG ATGTTCCTCC TGCACTACCA CCTGAACATC CACGACCTCT TCACATTTCT 
TGGGACATGG AAGACATGGA CAGATACAGA CTGCAATCCC TGCGACGAGA GAACAGGGCA 
CGTCAGAGAG CTAAAGGACC AGTAATGTCT CAGTATGATA ATGTTACACC AACCTTAGTG 
GAGGATGTAG CAGGCTTAGA TGTCATACAT CTCCGAAGCC GTTCTGATCC TGGTAAGACT 
CCTGGTCTTT TAAGTGTGGC TGAGAACAAA GACGTCCGTT ACCCAGGAAG GGCAGAAGGT 
GATGAGCATA CAACATATCC ACCTCCTGTT TTTGGAAATG GCCATCAAGA TAAGCCATCT 
CTTCCTCAAA AGCAAAGTGG ATCAAGCCGG GTTCGAATGC AACATGAAAT ATCTACTGAG 
CAGCGGACTC AACCTTCTCA AGATACTTTG CATAGGCAAC CCACAGAGGG ACGAAATGGT 
CCTCCAATTC CTCCTGTGGA CTACAGCTCT AAAGGAATGC CAAATCCCCC AGACCCTGCA 
TCATATCATT CCTCTGCAAA CAAATATAAC TTGACACAAC AGGAACCCAT GCGACTTATT 
CACAAAGAGC TTCGGCTCCC TGAGGATATT GAGCATTCAC ATACCAGGCC AGCTGACAAC 
CAACACCGTG ACTGTTACAG GGAGGAGCCA GCTCAGTTTC CCCTTACTGC ACCTCCTCCT 
AAACCAGAGA GGAGCCATAG CCTTCGAGGT CATAATCCAG ATGTTTTAGA AAGAGACCCT 
AGTATCTTCT ACCCCTACCA AACGCTTCAA GCCAAGAGAC ACAGCACAAT TAATGCAGTG 
TCACAATATG ATAATCTAGC TGATTACCAT TCTATACCCC ATCATCGAAC CACTAACCAG 
TCTACTCCAA ATGCCTTTAA CCTCCCACAT GGACGGACCT ATGCTACTGC CCTGGGCCAG 
GGTGCCTTTC TAGCTGCTGA ACTCGGCTTG CAAAGGCCTG AGACAAAAAT CCATGTGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916227.1
CDS	130..4932
Translation	MKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSGAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_004916170.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916170.3

ORF Insert Sequence:

ATGAAGTCCA GACCCAGCAA ACAGAAACTG AAGCAGAGAG GGATCCTGAG GGAGCGAGTG 
TTTGGTTGTG ACCTTGGGGA ACATCTGCTT AACTCTGGTC AGGATGTCCC CCAAGTCCTC 
AGGAGCTGCA CAGAGTTTAT AGAAAAACAT GGCATTGTTG ATGGAATCTA CCGTCTGTCT 
GGGATTGCAT CTAACATCCA GAAACTACGT CATGAATTTG ACTCTGAACA GATTCCAGAC 
CTGACTAAAG ATGTGTACAT CCAGGACATT CACTGTGTTG GCTCTCTCTG TAAACTCTAC 
TTCCGGGAAC TCCCAAACCC TCTGCTCACA TATCAGTTGT ATGAAAAGTT CTCTGATGCT 
GTGTCAGCGG CTACTGATGA AGAGAGACTG GTGAAAATTC ATGATGTTAT TCAACAGCTT 
CCCCCACCTC ATTACAGAAC ACTGGAGTTC CTTATGCGCC ACTTATCAAG ACTTGCGACA 
TACTGCTCTA TCACCAATAT GCACACTAAA AATTTGGCAA TTGTCTGGGC CCCTAACCTG 
CTCCGGTCAA AACAGATTGA GTCTGCATGT TTTAGCGGTA CAGCAGCTTT CATGGAAGTG 
CGCATACAGT CTGTGGTAGT TGAATTTATT CTAAACCATG TGGAGGTTCT CTTTAGTCCT 
AAACTTAGTT CTGTGATAAG GGATGGTGCT GGTCACACTT CTTTGTCTCG GCCCAAGTCA 
CTGTTGGTGT CATCTCCATC TACAAAACTA CTAAGCCTAG AGGAGGCTCA AGCAAGGACA 
CAGGCCCAAA TCAATTCCCC TGTTGTGGCT GACAGCAAGT ACATTGAGGT GGGAGAGGGA 
CCAGCTGCTT TGCAGGGCAG ATTCCACACT GTAATTGACT TCCCTTCTGA AAGGAAAAGA 
CCGCCAACCA AAATGAAAAA ATCTCCTGTT GGAAGCTGGC GTTCTTTTTT TAGCATAGGC 
AAATCATCAT CTTCAGCCTC CATGTCTAAG CGGAAACTTC AGCGTAATCC AAGTGAGCCT 
TTGGAAATGA AATCCATGGC ATTTGCAGGT GGAAGAGATA GCAGCACCAT GCGCTCAGCT 
AAAAGCGAGG AGTCTCTCAG TTCCTTGCAT GCAGCAGATG GAGAGTCGAA GCTATTTCGT 
CCACGTCGTC CTCGTTCCAG CAGCGATGCT CTGTCTGCTT CATATAATGG GGACCTTCTG 
GATAGCTGCA ACCGTTGCAA CTCCTATGAT AATCTGCCCC GAGACCAAGA CAGTGATGGA 
GATGAGGGTT TGATTCATGT GCCTGCTATG CTGTCAGGTC GCTCTCCTGA GGATGAAGAA 
CTCAGCCCAC CAGACATTGG TATGGCCAGT TTGGACTTTG ACCCAATGTC TTTTCAGTGC 
AGCCCTCCCC AGCTAGAATC TGAAGGACCA GACATTTTTT TTCAGCTTGA CATTTGTGCT 
AGCAATGGCA AGGATAAGGT GCAAAGTGGT GCACAAAACC TAGGCGGTGT CTCCAGCTCC 
GAACCTCACT CTCCTTTCCA AGAGAAAGTC GTTAGTCCAT TTCTATCGCC TGACCGCAGT 
TCTAGTACAG ATAAAGTATC CAAAACTACA TCATTTGCAG AAAAAGTTGT GCAGGCCTTG 
TCACCTAAAG TGGGGCGCAA AGCAGTTCGA TCTCCTCCAT TAACCATCTC AGAGCCTGTC 
ACCATCTCTC TCCCTAGTCG GGTATCGGAA ATGATTGGAA GTATGCAAGG CAATACTGCT 
GCTTCCCAGT CTTTAATTTG GTACAGAGAA AACAGTTTAA ATTCTAGAGA AACTGAAATG 
AATTATGTTT GTAAATCGGT GGATGCCAAC CTTCCATCCT TATTGCCAGC AACAGATCCA 
TGGTCTACAG TTACTCCTGT AATTACTCAA TGGAACCCAG ATGGATTACA GAGAGAAGAC 
AAAGCAGGAC CATTTACTCA TGGCTTTCCC CAGCCAAATA GTGACTTGGA ACAAGTAGAA 
AACTTCAACC TGGAACTTAG CACAACAAAT GTTGCTGCAG CATATCAGAA AGAAGATACA 
GAATTGTCGA GAGCAAATCA GAACAAATCC CACTCTTCTG TTTCTCCCCC TCAAGGAGCT 
TCTGCTTCAG AATCTCCACA GGAGCTAGCC CCTGTCAGTA GTGTTTCTAT CATTCCACCT 
CCACCTCCTC CAAAGAATCC TGCACGCATG CTGGCATTAG CACTGGCTGA ATCTGCTCAA 
CAGGCATCTG CTAATAAAAG ACCTCTTTAT ATGCAGTTCA TGGATCTACC CCCCCATCCT 
CCTCCACCAT CAGTACTAGA GGACAAACCA CTTCTTGAGT TATCTCCAAA AATGTCTCCA 
GAGCACCTCG TATCTGGTCC TGCAACACCA GCGACCAGTC CTCCAGTCAT ATCCAATAAA 
ACATCTCCAG CTACTCCACC AAGCACAACT TCATCATTTT CTATCACAAC AATTCATCAC 
AGTCCTGTGA AAAACCATAG TCCTCTGGCA GGACAGATGC CTACAGCAGT CACTCCTGGA 
CTCAGTCCTT CATCAAACCA GGTTCCTGTA TCCTTTCTAT CTGATGAAAT GCTACCTTCT 
GAAACAAATA AAATGAATTC TGTCATACTG AGTGAAGCAT TTACAACAGG AATTCCTGTT 
ACTCCACCAT CTGAAAAACC CAAGGAAATT TCTAGACCAC CACATCTTCA CCAGCGTTCT 
GAATCCTTTC CTTCACATCC TGCATATAGC AATGCAAAGC CCACTCCACC AGTTAGAACA 
ATGGATAGTC GACTGGCAAC TGCCATGCAT TCCAACTTCA ATGATGCCAT CACTGCTAAT 
AACTACCACT CCTTCCTGAA CACCATGATG CTTCCTTCTT CTCTAGAGGA TGTGCTACCC 
AGACACAACT ATTCTCATTC TTCTCATCTC AAGACAGAAA ATATAGATGA ACAGGGAGTT 
AATTACAGAC AACCTTATTT ATCCAACACC AAGCAAGAGG ACCCAGCTGA TGTGGGAGAT 
TTATACAGAC AGCAATATAT TTCTACTGGA AAGTCTGAGA ATGCTGAGAT AGGAAACACT 
TACCGGCACC CATATCCTTC CAATACTGAG CCAGAAAGTC TAAAGGAGCC ACTAGATCCT 
TTTCTGCATC ACAAGTCAGC AGTTGCTCCA AAGACATATA GAGCAGAAAC ACTTCTACCA 
CATATGCCTC CTCAAGTTTA TGGTTCAAGA TGTGATACTC CATCCTCAAC ATTTTATGGC 
ACCTACATAA ATCAGGCCAA ACACCCCCGC ACCAGGAACA GACCTGATTA TATGTCCTCT 
ATGAGCCCAG GTCTCCGTAG CTACTCAGAA GACACATCTT CCTACCCTAC TATTCGAAGG 
GTACAATCTC TGCATGTGCC TATGCAACCT CCGCCACCTC CTATCCGCAC TGTTCCAATT 
TCAAGAACAG AGGTTCCCCC AGATGATGAT CCAGCATACT GCCCAAGACC AGTTTATCAA 
TACAAACAAT GCCCCCAATT TAACTCTCAG TCAGATTACC ATGTTACCCA GCTGCAGCCT 
TACTTTGAAA ATGGACGAGT TCATTATCGC TACAGTCCCT ATTCAGGGGG CCCAAGTTAT 
TTTTCAGCAG ATAGCAATTT CTATGATATG GACCCCTACA GCACCTTACG TCTGCGACAA 
CTTCCTCTCT ACCCAAGCCG GGACTTTGCT TCTTACACCA CAAGATTGCA GCCCAAAAAC 
ACATATCGGT CACAAGGATT GCCCCCATAT CCCAGAGGAA CTCTACGGGA ACACAATTTT 
ATCAGTAGAG ATGTTCCTCC TGCACTACCA CCTGAACATC CACGACCTCT TCACATTTCT 
TGGGACATGG AAGACATGGA CAGATACAGA CTGCAATCCC TGCGACGAGA GAACAGGGCA 
CGTCAGAGAG CTAAAGGACC AGTAATGTCT CAGTATGATA ATGTTACACC AACCTTAGTG 
GAGGATGTAG CAGGCTTAGA TGTCATACAT CTCCGAAGCC GTTCTGATCC TGGTAAGACT 
CCTGGTCTTT TAAGTGTGGC TGAGAACAAA GACGTCCGTT ACCCAGGAAG GGCAGAAGGT 
GATGAGCATA CAACATATCC ACCTCCTGTT TTTGGAAATG GCCATCAAGA TAAGCCATCT 
CTTCCTCAAA AGCAAAGTGG ATCAAGCCGG GTTCGAATGC AACATGAAAT ATCTACTGAG 
CAGCGGACTC AACCTTCTCA AGATACTTTG CATAGGCAAC CCACAGAGGG ACGAAATGGT 
CCTCCAATTC CTCCTGTGGA CTACAGCTCT AAAGGAATGC CAAATCCCCC AGACCCTGCA 
TCATATCATT CCTCTGCAAA CAAATATAAC TTGACACAAC AGGAACCCAT GCGACTTATT 
CACAAAGAGC TTCGGCTCCC TGAGGATATT GAGCATTCAC ATACCAGGCC AGCTGACAAC 
CAACACCGTG ACTGTTACAG GGAGGAGCCA GCTCAGTTTC CCCTTACTGC ACCTCCTCCT 
AAACCAGAGA GGAGCCATAG CCTTCGAGGT CATAATCCAG ATGTTTTAGA AAGAGACCCT 
AGTATCTTCT ACCCCTACCA AACGCTTCAA GCCAAGAGAC ACAGCACAAT TAATGCAGTG 
TCACAATATG ATAATCTAGC TGATTACCAT TCTATACCCC ATCATCGAAC CACTAACCAG 
TCTACTCCAA ATGCCTTTAA CCTCCCACAT GGACGGACCT ATGCTACTGC CCTGGGCCAG 
GGTGCCTTTC TAGCTGCTGA ACTCGGCTTG CAAAGGCCTG AGACAAAAAT CCATGTGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32 ( XM_012967128.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822582.1 Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X8, mRNA.

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CloneID	OXa28809
Clone ID Related Accession (Same CDS sequence)	XM_012967128.1
Accession Version	XM_012967128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 32 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGAA TAATGAAGTC TTCACTGCAC TTAGAGGAGG ATGATTTTGT ACCCGAGTTG 
CCAAGGAGTA TCCATCCCCG GGAGAGGCCG GACTGGGAGG AGACTATTAG TGCCATGGCA 
CGAGGGGCAG ACATCCCAGT CCCAGGGGAA ATACCGACGA TGCCTACAGA AATCCCTCTT 
CGCTCCTGTG GCAGTACTGC CAGCATGAAG GTGAAAAATG TCAAGAAGTT ATCCTTCACG 
AAAGGGCATT TTCCTAAACT TGCAGAATGT GCACATTTCC ACTATGAAAA TGTAGACTTT 
GGCAGCATAC AGCTCACACT CACAGAAGAT CAAAATGAAG TGAGCAGAAA TGGCTGTGAA 
TCCAAGGAAC TTGTTTTCCT AGTACAGATT GCTTGTCAGG GCCGTAGCTG GATAGTTAAG 
CGGAGTTATG AAGATTTTCG GGTTCTGGAT AAACACCTTC ATCTGTGCAT ATATGATAGG 
CGCTTCTCTC AGCTCTCAGA GCTGCCCCGT TCCGACTCCG TAAAGGATAG TCCAGAGTTG 
GTCACACAGA TGCTGATGGC TTACCTATCG CGGCTGTCAG CCATCGCAGG TAACAAGATC 
AATTGTGGGC CAGCTCTGAC GTGGATGGAG ATCGATAATA AAGGCAATCA TTTGCTTGTG 
CACGAAGAGT CCTCCATAAA TGTTCCAGCC ATCGCTGCTG CCCATGTCAT AAAGCGATAT 
AATGCTCAGG CCCCAGATGA ACTCTCTTTT GAGGTGGGAG ACATAGTATC TGTAATTGAC 
ATGCCACCAA AAGAACTGAC CACGTGGTGG AGAGGAAAGC ATGGATTTCA AGTGGGTTTC 
TTTCCAAGTG AATGTGTGGA ATTAATAAAT GATAAAGTCC CCCAATCAAT GACAAACTCT 
GTGCCTAAAC CAGTGTCCCG GAAGCACGGC AAGCTCATCA CATTTCTAAG AACCTTCATG 
AAGTCCAGAC CCAGCAAACA GAAACTGAAG CAGAGAGGGA TCCTGAGGGA GCGAGTGTTT 
GGTTGTGACC TTGGGGAACA TCTGCTTAAC TCTGGTCAGG ATGTCCCCCA AGTCCTCAGG 
AGCTGCACAG AGTTTATAGA AAAACATGGC ATTGTTGATG GAATCTACCG TCTGTCTGGG 
ATTGCATCTA ACATCCAGAA ACTACGTCAT GAATTTGACT CTGAACAGAT TCCAGACCTG 
ACTAAAGATG TGTACATCCA GGACATTCAC TGTGTTGGCT CTCTCTGTAA ACTCTACTTC 
CGGGAACTCC CAAACCCTCT GCTCACATAT CAGTTGTATG AAAAGTTCTC TGATGCTGTG 
TCAGCGGCTA CTGATGAAGA GAGACTGGTG AAAATTCATG ATGTTATTCA ACAGCTTCCC 
CCACCTCATT ACAGAACACT GGAGTTCCTT ATGCGCCACT TATCAAGACT TGCGACATAC 
TGCTCTATCA CCAATATGCA CACTAAAAAT TTGGCAATTG TCTGGGCCCC TAACCTGCTC 
CGGTCAAAAC AGATTGAGTC TGCATGTTTT AGCGGTACAG CAGCTTTCAT GGAAGTGCGC 
ATACAGTCTG TGGTAGTTGA ATTTATTCTA AACCATGTGG AGGTTCTCTT TAGTCCTAAA 
CTTAGTTCTG TGATAAGGGA TGGTGCTGGT CACACTTCTT TGTCTCGGCC CAAGTCACTG 
TTGGTGTCAT CTCCATCTAC AAAACTACTA AGCCTAGAGG AGGCTCAAGC AAGGACACAG 
GCCCAAATCA ATTCCCCTGT TGTGGCTGAC AGCAAGTACA TTGAGGTGGG AGAGGGACCA 
GCTGCTTTGC AGGGCAGATT CCACACTGTA ATTGACTTCC CTTCTGAAAG GAAAAGACCG 
CCAACCAAAA TGAAAAAATC TCCTGTTGGA AGCTGGCGTT CTTTTTTTAG CATAGGCAAA 
TCATCATCTT CAGCCTCCAT GTCTAAGCGG AAACTTCAGC GTAATCCAAG TGAGCCTTTG 
GAAATGAAAT CCATGGCATT TGCAGGTGGA AGAGATAGCA GCACCATGCG CTCAGCTAAA 
AGCGAGGAGT CTCTCAGTTC CTTGCATGCA GCAGATGGAG AGTCGAAGCT ATTTCGTCCA 
CGTCGTCCTC GTTCCAGCAG CGATGCTCTG TCTGCTTCAT ATAATGGGGA CCTTCTGGAT 
AGCTGCAACC GTTGCAACTC CTATGATAAT CTGCCCCGAG ACCAAGACAG TGATGGAGAT 
GAGGGTTTGA TTCATGTGCC TGCTATGCTG TCAGGTCGCT CTCCTGAGGA TGAAGAACTC 
AGCCCACCAG ACATTGGTAT GGCCAGTTTG GACTTTGACC CAATGTCTTT TCAGTGCAGC 
CCTCCCCAGC TAGAATCTGA AGGACCAGAC ATTTTTTTTC AGCTTGACAT TTGTGCTAGC 
AATGGCAAGG ATAAGGTGCA AAGTGGTGCA CAAAACCTAG GCGGTGTCTC CAGCTCCGAA 
CCTCACTCTC CTTTCCAAGA GAAAGTCGTT AGTCCATTTC TATCGCCTGA CCGCAGTTCT 
AGTACAGATA AAGTATCCAA AACTACATCA TTTGCAGAAA AAGTTGTGCA GGCCTTGTCA 
CCTAAAGTGG GGCGCAAAGC AGTTCGATCT CCTCCATTAA CCATCTCAGA GCCTGTCACC 
ATCTCTCTCC CTAGTCGGGT ATCGGAAATG ATTGGAAGTA TGCAAGGCAA TACTGCTGCT 
TCCCAGTCTT TAATTTGGTA CAGAGAAAAC AGTTTAAATT CTAGAGAAAC TGAAATGAAT 
TATGTTTGTA AATCGGTGGA TGCCAACCTT CCATCCTTAT TGCCAGCAAC AGATCCATGG 
TCTACAGTTA CTCCTGTAAT TACTCAATGG AACCCAGATG GATTACAGAG AGAAGACAAA 
GCAGGACCAT TTACTCATGG CTTTCCCCAG CCAAATAGTG ACTTGGAACA AGTAGAAAAC 
TTCAACCTGG AACTTAGCAC AACAAATGTT GCTGCAGCAT ATCAGAAAGA AGATACAGAA 
TTGTCGAGAG CAAATCAGAA CAAATCCCAC TCTTCTGTTT CTCCCCCTCA AGGAGCTTCT 
GCTTCAGAAT CTCCACAGGA GCTAGCCCCT GTCAGTAGTG TTTCTATCAT TCCACCTCCA 
CCTCCTCCAA AGAATCCTGC ACGCATGCTG GCATTAGCAC TGGCTGAATC TGCTCAACAG 
GCATCTGCTA ATAAAAGACC TCTTTATATG CAGTTCATGG ATCTACCCCC CCATCCTCCT 
CCACCATCAG TACTAGAGGA CAAACCACTT CTTGAGTTAT CTCCAAAAAT GTCTCCAGAG 
CACCTCGTAT CTGGTCCTGC AACACCAGCG ACCAGTCCTC CAGTCATATC CAATAAAACA 
TCTCCAGCTA CTCCACCAAG CACAACTTCA TCATTTTCTA TCACAACAAT TCATCACAGT 
CCTGTGAAAA ACCATAGTCC TCTGGCAGGA CAGATGCCTA CAGCAGTCAC TCCTGGACTC 
AGTCCTTCAT CAAACCAGGT TCCTGTATCC TTTCTATCTG ATGAAATGCT ACCTTCTGAA 
ACAAATAAAA TGAATTCTGT CATACTGAGT GAAGCATTTA CAACAGGAAT TCCTGTTACT 
CCACCATCTG AAAAACCCAA GGAAATTTCT AGACCACCAC ATCTTCACCA GCGTTCTGAA 
TCCTTTCCTT CACATCCTGC ATATAGCAAT GCAAAGCCCA CTCCACCAGT TAGAACAATG 
GATAGTCGAC TGGCAACTGC CATGCATTCC AACTTCAATG ATGCCATCAC TGCTAATAAC 
TACCACTCCT TCCTGAACAC CATGATGCTT CCTTCTTCTC TAGAGGATGT GCTACCCAGA 
CACAACTATT CTCATTCTTC TCATCTCAAG ACAGAAAATA TAGATGAACA GGGAGTTAAT 
TACAGACAAC CTTATTTATC CAACACCAAG CAAGAGGACC CAGCTGATGT GGGAGATTTA 
TACAGACAGC AATATATTTC TACTGGAAAG TCTGAGAATG CTGAGATAGG AAACACTTAC 
CGGCACCCAT ATCCTTCCAA TACTGAGCCA GAAAGTCTAA AGGAGCCACT AGATCCTTTT 
CTGCATCACA AGTCAGCAGT TGCTCCAAAG ACATATAGAG CAGAAACACT TCTACCACAT 
ATGCCTCCTC AAGTTTATGG TTCAAGATGT GATACTCCAT CCTCAACATT TTATGGCACC 
TACATAAATC AGGCCAAACA CCCCCGCACC AGGAACAGAC CTGATTATAT GTCCTCTATG 
AGCCCAGGTC TCCGTAGCTA CTCAGAAGAC ACATCTTCCT ACCCTACTAT TCGAAGGGTA 
CAATCTCTGC ATGTGCCTAT GCAACCTCCG CCACCTCCTA TCCGCACTGT TCCAATTTCA 
AGAACAGAGG TTCCCCCAGA TGATGATCCA GCATACTGCC CAAGACCAGT TTATCAATAC 
AAACAATGCC CCCAATTTAA CTCTCAGTCA GATTACCATG TTACCCAGCT GCAGCCTTAC 
TTTGAAAATG GACGAGTTCA TTATCGCTAC AGTCCCTATT CAGGGGGCCC AAGTTATTTT 
TCAGCAGATA GCAATTTCTA TGATATGGAC CCCTACAGCA CCTTACGTCT GCGACAACTT 
CCTCTCTACC CAAGCCGGGA CTTTGCTTCT TACACCACAA GATTGCAGCC CAAAAACACA 
TATCGGTCAC AAGGATTGCC CCCATATCCC AGAGGAACTC TACGGGAACA CAATTTTATC 
AGTAGAGATG TTCCTCCTGC ACTACCACCT GAACATCCAC GACCTCTTCA CATTTCTTGG 
GACATGGAAG ACATGGACAG ATACAGACTG CAATCCCTGC GACGAGAGAA CAGGGCACGT 
CAGAGAGCTA AAGGACCAGT AATGTCTCAG TATGATAATG TTACACCAAC CTTAGTGGAG 
GATGTAGCAG GCTTAGATGT CATACATCTC CGAAGCCGTT CTGATCCTGG TAAGACTCCT 
GGTCTTTTAA GTGTGGCTGA GAACAAAGAC GTCCGTTACC CAGGAAGGGC AGAAGGTGAT 
GAGCATACAA CATATCCACC TCCTGTTTTT GGAAATGGCC ATCAAGATAA GCCATCTCTT 
CCTCAAAAGC AAAGTGGATC AAGCCGGGTT CGAATGCAAC ATGAAATATC TACTGAGCAG 
CGGACTCAAC CTTCTCAAGA TACTTTGCAT AGGCAACCCA CAGAGGGACG AAATGGTCCT 
CCAATTCCTC CTGTGGACTA CAGCTCTAAA GGAATGCCAA ATCCCCCAGA CCCTGCATCA 
TATCATTCCT CTGCAAACAA ATATAACTTG ACACAACAGG AACCCATGCG ACTTATTCAC 
AAAGAGCTTC GGCTCCCTGA GGATATTGAG CATTCACATA CCAGGCCAGC TGACAACCAA 
CACCGTGACT GTTACAGGGA GGAGCCAGCT CAGTTTCCCC TTACTGCACC TCCTCCTAAA 
CCAGAGAGGA GCCATAGCCT TCGAGGTCAT AATCCAGATG TTTTAGAAAG AGACCCTAGT 
ATCTTCTACC CCTACCAAAC GCTTCAAGCC AAGAGACACA GCACAATTAA TGCAGTGTCA 
CAATATGATA ATCTAGCTGA TTACCATTCT ATACCCCATC ATCGAACCAC TAACCAGTCT 
ACTCCAAATG CCTTTAACCT CCCACATGGA CGGACCTATG CTACTGCCCT GGGCCAGGGT 
GCCTTTCTAG CTGCTGAACT CGGCTTGCAA AGGCCTGAGA CAAAAATCCA TGTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822582.1
CDS	201..5960
Translation	MYRIMKSSLHLEEDDFVPELPRSIHPRERPDWEETISAMARGADIPVPGEIPTMPTEIPLRSCGSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGSIQLTLTEDQNEVSRNGCESKELVFLVQIACQGRSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLSELPRSDSVKDSPELVTQMLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINVPAIAAAHVIKRYNAQAPDELSFEVGDIVSVIDMPPKELTTWWRGKHGFQVGFFPSECVELINDKVPQSMTNSVPKPVSRKHGKLITFLRTFMKSRPSKQKLKQRGILRERVFGCDLGEHLLNSGQDVPQVLRSCTEFIEKHGIVDGIYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLVKIHDVIQQLPPPHYRTLEFLMRHLSRLATYCSITNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVEVLFSPKLSSVIRDGAGHTSLSRPKSLLVSSPSTKLLSLEEAQARTQAQINSPVVADSKYIEVGEGPAALQGRFHTVIDFPSERKRPPTKMKKSPVGSWRSFFSIGKSSSSASMSKRKLQRNPSEPLEMKSMAFAGGRDSSTMRSAKSEESLSSLHAADGESKLFRPRRPRSSSDALSASYNGDLLDSCNRCNSYDNLPRDQDSDGDEGLIHVPAMLSGRSPEDEELSPPDIGMASLDFDPMSFQCSPPQLESEGPDIFFQLDICASNGKDKVQSGAQNLGGVSSSEPHSPFQEKVVSPFLSPDRSSSTDKVSKTTSFAEKVVQALSPKVGRKAVRSPPLTISEPVTISLPSRVSEMIGSMQGNTAASQSLIWYRENSLNSRETEMNYVCKSVDANLPSLLPATDPWSTVTPVITQWNPDGLQREDKAGPFTHGFPQPNSDLEQVENFNLELSTTNVAAAYQKEDTELSRANQNKSHSSVSPPQGASASESPQELAPVSSVSIIPPPPPPKNPARMLALALAESAQQASANKRPLYMQFMDLPPHPPPPSVLEDKPLLELSPKMSPEHLVSGPATPATSPPVISNKTSPATPPSTTSSFSITTIHHSPVKNHSPLAGQMPTAVTPGLSPSSNQVPVSFLSDEMLPSETNKMNSVILSEAFTTGIPVTPPSEKPKEISRPPHLHQRSESFPSHPAYSNAKPTPPVRTMDSRLATAMHSNFNDAITANNYHSFLNTMMLPSSLEDVLPRHNYSHSSHLKTENIDEQGVNYRQPYLSNTKQEDPADVGDLYRQQYISTGKSENAEIGNTYRHPYPSNTEPESLKEPLDPFLHHKSAVAPKTYRAETLLPHMPPQVYGSRCDTPSSTFYGTYINQAKHPRTRNRPDYMSSMSPGLRSYSEDTSSYPTIRRVQSLHVPMQPPPPPIRTVPISRTEVPPDDDPAYCPRPVYQYKQCPQFNSQSDYHVTQLQPYFENGRVHYRYSPYSGGPSYFSADSNFYDMDPYSTLRLRQLPLYPSRDFASYTTRLQPKNTYRSQGLPPYPRGTLREHNFISRDVPPALPPEHPRPLHISWDMEDMDRYRLQSLRRENRARQRAKGPVMSQYDNVTPTLVEDVAGLDVIHLRSRSDPGKTPGLLSVAENKDVRYPGRAEGDEHTTYPPPVFGNGHQDKPSLPQKQSGSSRVRMQHEISTEQRTQPSQDTLHRQPTEGRNGPPIPPVDYSSKGMPNPPDPASYHSSANKYNLTQQEPMRLIHKELRLPEDIEHSHTRPADNQHRDCYREEPAQFPLTAPPPKPERSHSLRGHNPDVLERDPSIFYPYQTLQAKRHSTINAVSQYDNLADYHSIPHHRTTNQSTPNAFNLPHGRTYATALGQGAFLAAELGLQRPETKIHVE

Target ORF information:

RefSeq Version	XM_012967128.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 32 (arhgap32), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967128.1

ORF Insert Sequence:

ATGTACAGAA TAATGAAGTC TTCACTGCAC TTAGAGGAGG ATGATTTTGT ACCCGAGTTG 
CCAAGGAGTA TCCATCCCCG GGAGAGGCCG GACTGGGAGG AGACTATTAG TGCCATGGCA 
CGAGGGGCAG ACATCCCAGT CCCAGGGGAA ATACCGACGA TGCCTACAGA AATCCCTCTT 
CGCTCCTGTG GCAGTACTGC CAGCATGAAG GTGAAAAATG TCAAGAAGTT ATCCTTCACG 
AAAGGGCATT TTCCTAAACT TGCAGAATGT GCACATTTCC ACTATGAAAA TGTAGACTTT 
GGCAGCATAC AGCTCACACT CACAGAAGAT CAAAATGAAG TGAGCAGAAA TGGCTGTGAA 
TCCAAGGAAC TTGTTTTCCT AGTACAGATT GCTTGTCAGG GCCGTAGCTG GATAGTTAAG 
CGGAGTTATG AAGATTTTCG GGTTCTGGAT AAACACCTTC ATCTGTGCAT ATATGATAGG 
CGCTTCTCTC AGCTCTCAGA GCTGCCCCGT TCCGACTCCG TAAAGGATAG TCCAGAGTTG 
GTCACACAGA TGCTGATGGC TTACCTATCG CGGCTGTCAG CCATCGCAGG TAACAAGATC 
AATTGTGGGC CAGCTCTGAC GTGGATGGAG ATCGATAATA AAGGCAATCA TTTGCTTGTG 
CACGAAGAGT CCTCCATAAA TGTTCCAGCC ATCGCTGCTG CCCATGTCAT AAAGCGATAT 
AATGCTCAGG CCCCAGATGA ACTCTCTTTT GAGGTGGGAG ACATAGTATC TGTAATTGAC 
ATGCCACCAA AAGAACTGAC CACGTGGTGG AGAGGAAAGC ATGGATTTCA AGTGGGTTTC 
TTTCCAAGTG AATGTGTGGA ATTAATAAAT GATAAAGTCC CCCAATCAAT GACAAACTCT 
GTGCCTAAAC CAGTGTCCCG GAAGCACGGC AAGCTCATCA CATTTCTAAG AACCTTCATG 
AAGTCCAGAC CCAGCAAACA GAAACTGAAG CAGAGAGGGA TCCTGAGGGA GCGAGTGTTT 
GGTTGTGACC TTGGGGAACA TCTGCTTAAC TCTGGTCAGG ATGTCCCCCA AGTCCTCAGG 
AGCTGCACAG AGTTTATAGA AAAACATGGC ATTGTTGATG GAATCTACCG TCTGTCTGGG 
ATTGCATCTA ACATCCAGAA ACTACGTCAT GAATTTGACT CTGAACAGAT TCCAGACCTG 
ACTAAAGATG TGTACATCCA GGACATTCAC TGTGTTGGCT CTCTCTGTAA ACTCTACTTC 
CGGGAACTCC CAAACCCTCT GCTCACATAT CAGTTGTATG AAAAGTTCTC TGATGCTGTG 
TCAGCGGCTA CTGATGAAGA GAGACTGGTG AAAATTCATG ATGTTATTCA ACAGCTTCCC 
CCACCTCATT ACAGAACACT GGAGTTCCTT ATGCGCCACT TATCAAGACT TGCGACATAC 
TGCTCTATCA CCAATATGCA CACTAAAAAT TTGGCAATTG TCTGGGCCCC TAACCTGCTC 
CGGTCAAAAC AGATTGAGTC TGCATGTTTT AGCGGTACAG CAGCTTTCAT GGAAGTGCGC 
ATACAGTCTG TGGTAGTTGA ATTTATTCTA AACCATGTGG AGGTTCTCTT TAGTCCTAAA 
CTTAGTTCTG TGATAAGGGA TGGTGCTGGT CACACTTCTT TGTCTCGGCC CAAGTCACTG 
TTGGTGTCAT CTCCATCTAC AAAACTACTA AGCCTAGAGG AGGCTCAAGC AAGGACACAG 
GCCCAAATCA ATTCCCCTGT TGTGGCTGAC AGCAAGTACA TTGAGGTGGG AGAGGGACCA 
GCTGCTTTGC AGGGCAGATT CCACACTGTA ATTGACTTCC CTTCTGAAAG GAAAAGACCG 
CCAACCAAAA TGAAAAAATC TCCTGTTGGA AGCTGGCGTT CTTTTTTTAG CATAGGCAAA 
TCATCATCTT CAGCCTCCAT GTCTAAGCGG AAACTTCAGC GTAATCCAAG TGAGCCTTTG 
GAAATGAAAT CCATGGCATT TGCAGGTGGA AGAGATAGCA GCACCATGCG CTCAGCTAAA 
AGCGAGGAGT CTCTCAGTTC CTTGCATGCA GCAGATGGAG AGTCGAAGCT ATTTCGTCCA 
CGTCGTCCTC GTTCCAGCAG CGATGCTCTG TCTGCTTCAT ATAATGGGGA CCTTCTGGAT 
AGCTGCAACC GTTGCAACTC CTATGATAAT CTGCCCCGAG ACCAAGACAG TGATGGAGAT 
GAGGGTTTGA TTCATGTGCC TGCTATGCTG TCAGGTCGCT CTCCTGAGGA TGAAGAACTC 
AGCCCACCAG ACATTGGTAT GGCCAGTTTG GACTTTGACC CAATGTCTTT TCAGTGCAGC 
CCTCCCCAGC TAGAATCTGA AGGACCAGAC ATTTTTTTTC AGCTTGACAT TTGTGCTAGC 
AATGGCAAGG ATAAGGTGCA AAGTGGTGCA CAAAACCTAG GCGGTGTCTC CAGCTCCGAA 
CCTCACTCTC CTTTCCAAGA GAAAGTCGTT AGTCCATTTC TATCGCCTGA CCGCAGTTCT 
AGTACAGATA AAGTATCCAA AACTACATCA TTTGCAGAAA AAGTTGTGCA GGCCTTGTCA 
CCTAAAGTGG GGCGCAAAGC AGTTCGATCT CCTCCATTAA CCATCTCAGA GCCTGTCACC 
ATCTCTCTCC CTAGTCGGGT ATCGGAAATG ATTGGAAGTA TGCAAGGCAA TACTGCTGCT 
TCCCAGTCTT TAATTTGGTA CAGAGAAAAC AGTTTAAATT CTAGAGAAAC TGAAATGAAT 
TATGTTTGTA AATCGGTGGA TGCCAACCTT CCATCCTTAT TGCCAGCAAC AGATCCATGG 
TCTACAGTTA CTCCTGTAAT TACTCAATGG AACCCAGATG GATTACAGAG AGAAGACAAA 
GCAGGACCAT TTACTCATGG CTTTCCCCAG CCAAATAGTG ACTTGGAACA AGTAGAAAAC 
TTCAACCTGG AACTTAGCAC AACAAATGTT GCTGCAGCAT ATCAGAAAGA AGATACAGAA 
TTGTCGAGAG CAAATCAGAA CAAATCCCAC TCTTCTGTTT CTCCCCCTCA AGGAGCTTCT 
GCTTCAGAAT CTCCACAGGA GCTAGCCCCT GTCAGTAGTG TTTCTATCAT TCCACCTCCA 
CCTCCTCCAA AGAATCCTGC ACGCATGCTG GCATTAGCAC TGGCTGAATC TGCTCAACAG 
GCATCTGCTA ATAAAAGACC TCTTTATATG CAGTTCATGG ATCTACCCCC CCATCCTCCT 
CCACCATCAG TACTAGAGGA CAAACCACTT CTTGAGTTAT CTCCAAAAAT GTCTCCAGAG 
CACCTCGTAT CTGGTCCTGC AACACCAGCG ACCAGTCCTC CAGTCATATC CAATAAAACA 
TCTCCAGCTA CTCCACCAAG CACAACTTCA TCATTTTCTA TCACAACAAT TCATCACAGT 
CCTGTGAAAA ACCATAGTCC TCTGGCAGGA CAGATGCCTA CAGCAGTCAC TCCTGGACTC 
AGTCCTTCAT CAAACCAGGT TCCTGTATCC TTTCTATCTG ATGAAATGCT ACCTTCTGAA 
ACAAATAAAA TGAATTCTGT CATACTGAGT GAAGCATTTA CAACAGGAAT TCCTGTTACT 
CCACCATCTG AAAAACCCAA GGAAATTTCT AGACCACCAC ATCTTCACCA GCGTTCTGAA 
TCCTTTCCTT CACATCCTGC ATATAGCAAT GCAAAGCCCA CTCCACCAGT TAGAACAATG 
GATAGTCGAC TGGCAACTGC CATGCATTCC AACTTCAATG ATGCCATCAC TGCTAATAAC 
TACCACTCCT TCCTGAACAC CATGATGCTT CCTTCTTCTC TAGAGGATGT GCTACCCAGA 
CACAACTATT CTCATTCTTC TCATCTCAAG ACAGAAAATA TAGATGAACA GGGAGTTAAT 
TACAGACAAC CTTATTTATC CAACACCAAG CAAGAGGACC CAGCTGATGT GGGAGATTTA 
TACAGACAGC AATATATTTC TACTGGAAAG TCTGAGAATG CTGAGATAGG AAACACTTAC 
CGGCACCCAT ATCCTTCCAA TACTGAGCCA GAAAGTCTAA AGGAGCCACT AGATCCTTTT 
CTGCATCACA AGTCAGCAGT TGCTCCAAAG ACATATAGAG CAGAAACACT TCTACCACAT 
ATGCCTCCTC AAGTTTATGG TTCAAGATGT GATACTCCAT CCTCAACATT TTATGGCACC 
TACATAAATC AGGCCAAACA CCCCCGCACC AGGAACAGAC CTGATTATAT GTCCTCTATG 
AGCCCAGGTC TCCGTAGCTA CTCAGAAGAC ACATCTTCCT ACCCTACTAT TCGAAGGGTA 
CAATCTCTGC ATGTGCCTAT GCAACCTCCG CCACCTCCTA TCCGCACTGT TCCAATTTCA 
AGAACAGAGG TTCCCCCAGA TGATGATCCA GCATACTGCC CAAGACCAGT TTATCAATAC 
AAACAATGCC CCCAATTTAA CTCTCAGTCA GATTACCATG TTACCCAGCT GCAGCCTTAC 
TTTGAAAATG GACGAGTTCA TTATCGCTAC AGTCCCTATT CAGGGGGCCC AAGTTATTTT 
TCAGCAGATA GCAATTTCTA TGATATGGAC CCCTACAGCA CCTTACGTCT GCGACAACTT 
CCTCTCTACC CAAGCCGGGA CTTTGCTTCT TACACCACAA GATTGCAGCC CAAAAACACA 
TATCGGTCAC AAGGATTGCC CCCATATCCC AGAGGAACTC TACGGGAACA CAATTTTATC 
AGTAGAGATG TTCCTCCTGC ACTACCACCT GAACATCCAC GACCTCTTCA CATTTCTTGG 
GACATGGAAG ACATGGACAG ATACAGACTG CAATCCCTGC GACGAGAGAA CAGGGCACGT 
CAGAGAGCTA AAGGACCAGT AATGTCTCAG TATGATAATG TTACACCAAC CTTAGTGGAG 
GATGTAGCAG GCTTAGATGT CATACATCTC CGAAGCCGTT CTGATCCTGG TAAGACTCCT 
GGTCTTTTAA GTGTGGCTGA GAACAAAGAC GTCCGTTACC CAGGAAGGGC AGAAGGTGAT 
GAGCATACAA CATATCCACC TCCTGTTTTT GGAAATGGCC ATCAAGATAA GCCATCTCTT 
CCTCAAAAGC AAAGTGGATC AAGCCGGGTT CGAATGCAAC ATGAAATATC TACTGAGCAG 
CGGACTCAAC CTTCTCAAGA TACTTTGCAT AGGCAACCCA CAGAGGGACG AAATGGTCCT 
CCAATTCCTC CTGTGGACTA CAGCTCTAAA GGAATGCCAA ATCCCCCAGA CCCTGCATCA 
TATCATTCCT CTGCAAACAA ATATAACTTG ACACAACAGG AACCCATGCG ACTTATTCAC 
AAAGAGCTTC GGCTCCCTGA GGATATTGAG CATTCACATA CCAGGCCAGC TGACAACCAA 
CACCGTGACT GTTACAGGGA GGAGCCAGCT CAGTTTCCCC TTACTGCACC TCCTCCTAAA 
CCAGAGAGGA GCCATAGCCT TCGAGGTCAT AATCCAGATG TTTTAGAAAG AGACCCTAGT 
ATCTTCTACC CCTACCAAAC GCTTCAAGCC AAGAGACACA GCACAATTAA TGCAGTGTCA 
CAATATGATA ATCTAGCTGA TTACCATTCT ATACCCCATC ATCGAACCAC TAACCAGTCT 
ACTCCAAATG CCTTTAACCT CCCACATGGA CGGACCTATG CTACTGCCCT GGGCCAGGGT 
GCCTTTCTAG CTGCTGAACT CGGCTTGCAA AGGCCTGAGA CAAAAATCCA TGTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	arhgap32
Entrez Gene ID	734056
Full Name	Rho GTPase activating protein 32
Synonyms	gc-gap,grit,p200rhogap,p250gap,px-rics,rics
General protein information	Preferred Names Rho GTPase activating protein 32 Names rho GTPase-activating protein 32
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Bos taurus (cattle)	ARHGAP32	NP_001179016.1
Danio rerio (zebrafish)	im:7150060	XP_005161318.1
Pan troglodytes (chimpanzee)	ARHGAP32	XP_003313461.2
Canis lupus familiaris (dog)	ARHGAP32	XP_546401.3
Mus musculus (house mouse)	Arhgap32	NP_001182561.1
Xenopus tropicalis (tropical clawed frog)	arhgap32	XP_004916220.1
Homo sapiens (human)	ARHGAP32	NP_001136157.1
Rattus norvegicus (Norway rat)	Arhgap32	XP_006242835.1
	ARHGAP32	XP_001111190.2
Gallus gallus (chicken)	ARHGAP32	XP_004948019.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

arhgap32 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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