ddx54 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ddx54
Entrez Gene ID	733994
Full Name	DEAD-box helicase 54
General protein information	Preferred Names DEAD-box helicase 54 Names ATP-dependent RNA helicase DDX54 DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018089863.1	XP_017945352.1	ATP-dependent RNA helicase DDX54 isoform X2
XM_002934334.4	XP_002934380.1	ATP-dependent RNA helicase DDX54 isoform X1
XM_018089863.2	XP_017945352.1	ATP-dependent RNA helicase DDX54 isoform X2
XM_002934334.5	XP_002934380.1	ATP-dependent RNA helicase DDX54 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ddx54	NP_082317.1
Danio rerio (zebrafish)	ddx54	NP_775375.1
	DDX54	XP_001111246.1
Gallus gallus (chicken)	DDX54	XP_003642273.2
Caenorhabditis elegans (roundworm)	Y94H6A.5	NP_741347.2
Saccharomyces cerevisiae (baker's yeast)	DBP10	NP_010253.2
Homo sapiens (human)	DDX54	NP_001104792.1
Pan troglodytes (chimpanzee)	DDX54	XP_001152281.1
Bos taurus (cattle)	DDX54	XP_005218062.1
Neurospora crassa	NCU07712	XP_962298.1
Arabidopsis thaliana (thale cress)	AT1G77030	NP_177829.5
Xenopus tropicalis (tropical clawed frog)	ddx54	XP_002934380.1
Canis lupus familiaris (dog)	DDX54	XP_005636568.1
Rattus norvegicus (Norway rat)	Ddx54	NP_001178477.1
Drosophila melanogaster (fruit fly)	CG32344	NP_612028.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007511	XP_308366.3
Kluyveromyces lactis	KLLA0F24684g	XP_456179.1
Eremothecium gossypii	AGOS_AEL086W	NP_984775.1
Schizosaccharomyces pombe (fission yeast)	dbp10	NP_592919.1
Magnaporthe oryzae (rice blast fungus)	MGG_04179	XP_003719645.1
Oryza sativa (rice)	Os08g0416100	NP_001061808.1

Gene Ontology

The following ddx54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ddx54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa03708	XM_002934334.5 Latest version!	Xenopus tropicalis DEAD-box helicase 54 (ddx54), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa42139	XM_018089863.2 Latest version!	Xenopus tropicalis DEAD-box helicase 54 (ddx54), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OXa42139	XM_018089863.1	Xenopus tropicalis DEAD-box helicase 54 (ddx54), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OXa03708	XM_002934334.4	Xenopus tropicalis DEAD-box helicase 54 (ddx54), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ddx54 ( XM_002934334.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934380.1 Xenopus tropicalis DEAD-box helicase 54 (ddx54), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa03708
Clone ID Related Accession (Same CDS sequence)	XM_002934334.4 , XM_002934334.5
Accession Version	XM_002934334.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-dependent RNA helicase DDX54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934334.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGA AAAAACGTAA GGGACAGCGA TCTCGGAAAT GCTGTCAAAA CCAAGAGAGC 
GACGAAAGCG ACACAGTGGG ATTTCGTGTC AAAGCTGCAC CCCAAAAGGA GGATGGAGTA 
AAACTTCCAG CATTTCCCAC TGCAGATGCA ACTTCAGATA CAGAACTGGA CACCAGAGAA 
ATGGTGCGAG CACAGAATAA GAAGAAAAAG AAATCTGGCG GGTTTCAGTC CATGGGTCTT 
AGTTACCCAG TCTTTAAAGG AGTGATGAAG AAAGGTTACA AGGTTCCTAC TCCCATTCAG 
AGAAAGGTGA TTCCAGTCAT TCTTGATGGC AAGGATGTGG TAGCAATGGC TAGAACGGGC 
AGTGGAAAGA CTGCCTGTTT CCTTATCCCT ATGTTTGAGA AGTTAAAGGC TCACAGTGCC 
CAAACAGGAG TTCGTGGGCT TATCCTTTCA CCAACTAGAG AGCTGGCCTT GCAGACTCTG 
AAATTCACAA AGGAGCTTGG TAAATTCACT GGTCTGAAGA CTGCTCTGAT TTTAGGTGGT 
GACAAAATGG AAGATCAGTT TGCTGCTCTG CATGAAAATC CAGATATAAT TATTGCCACT 
CCTGGTCGAC TGATGCATGT GGCAATTGAG ATGAACCTGA AACTTCGCAC TGTGGAATAT 
GTGGTGTTTG ACGAAGCTGA CAGACTCTTT GAGATGGGAT TTGCAGAGCA GCTGCAAGAG 
ATAATCTCTC GACTACCAGA GACACGCCAG ACACTCCTTT TTTCCGCCAC TCTGCCCAAG 
ATGCTTCTAG AGTTTGCCCG AGCTGGCCTT ACTGAGCCTG TCTTAATACG TTTGGATGTT 
GATACAAAGC TGAGTGACCA GCTGAAGTTG TCCTTCTTCA ATGTGCGAGT AGAGGACAAA 
CCTGCTGTGC TTCTGCATCT CCTGCGCTGT GTTGTAAAGC CTCAAGAACA AACAGTCATT 
TTTGTTGCCA CTAAGCACCA TGCAGAATAC CTGAGAGAGT TGCTGGACAT GCAGGGAATT 
CCATGCTCTC ACATATACAG TGCTCTGGAT CAGACAGCCA GGAAGATAAA CCTGGGCCTT 
TTTTTGCATG GGAAGGTGCG TGCACTGCTT GTCACAGATG TGGCTGCTCG AGGTATTGAC 
ATACCAATGT TAGACAACGT CATAAACTAC AACTTCCCTC CGAAAGCCAA GCTTTTCCTG 
CATCGTGTTG GGCGTGTGGC AAGAGCAGGG CGAAGTGGGA CTGCTTACTC TCTTATTGCT 
CCTGATGAAA TTCCCTATGT GTATGATCTC CATCTCTTTC TTGGGCGGCC GCTTAAACTG 
GCAACAGCAC AAATGATGGA GACAGGAGCA GACACAGATG GGGTACTGGG GCGAGTACCA 
CAGAGTCTGA TTGATGATGA AGATGCCTTG CTTATTACGG ATCGTGAGCG ATCGCTGGAG 
CTGCAGAACT TGCACAACGT GGCAGAGAAT GCCTACAAAC AGTACATTAA ATCCAGGCCA 
ACTCCCTCTC CCGAGTCCAT CAAACGAGTC AAGGATGCTA ACTATAACCT GTTGGGGGTT 
CACCCACTCT TTTGGTCTCA GCTTGGTGAA GAAGAGATGC AGAGGCTGAA GTTTGTGGAT 
GGCATCAAAT CTTACAAATC TAAAGCAACC ATATTTGAAA TTAACGCTAC TAATAAAACC 
AATGCAAGTG AAGTTATGCG AGCCAAGAGA AGTCATGATG GGAAGGTCAT CGCTAAGTTC 
CAGCGCCTGC AGGAGGAGAA AAGTGCAACA GTGCAGACTT TGACCAGTGG CATCTCCAGC 
CTTAGCTGCA AACAAGAGAG TGATGAGGAG AACATGGAGG GAGTATTTTC GCAAGTGCTT 
GGAAAAAGGA AAATTACTGA CAAAGATGAA GCTGAAGGCA ACAAGAAAAG GAAAGCAGGA 
ACTTCTCATG ACACTGACTA TTACATCCCA TACAGACCAA AGGATTTTGA GAGTGAGAGG 
GGGCTCAGCA TTGGGGCCAG CAGCTTTGAG CAGGCAGCTG CAGGGGCTGT GTTGGACCTA 
TTGGGAGACC AGACGGAAGA TCTCAGGAAG AACAAACAAG TCATGAAGTG GGATAGAAAG 
AAAAAAAAGT TTGTGGGAAC AGGTGGACCA GAAGATAAAA AGAAAATTAA GACTGAGAGT 
GGACGACTCA TTAGTAGCTC CTATAAAAAA AACCTATATG AAGGCTGGAA GAAGAAATAC 
AAGATTGATG ACCAAGATTC TGAGGAAGAG GACGAGGAAA GAGATCAGAG GGCAGCTGCG 
GGGCGCAGGA AAGGGCGAGG TGGGCGTGGC CCCCAAAGAA GCTCTCATTC AAGTTTAGGG 
CCACAGGGGA AAGCCAAATC AGAGCTGCGT AGCAAACAGC AAATCCTCAA GACGAGAAAG 
GCACAAGCTA AACAGAACTT CTTGCAAAAA GGCGGTTTGA AGAAGATGAA AAACAAGACA 
AGACACCTAG CCAATCATAT GAAGAAATCT GCTTTTGGAA GAGGGAAAAG CCAAGGTTTC 
AAGAAAGGAA AATCAAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934380.1
CDS	140..2683
Translation	MAQKKRKGQRSRKCCQNQESDESDTVGFRVKAAPQKEDGVKLPAFPTADATSDTELDTREMVRAQNKKKKKSGGFQSMGLSYPVFKGVMKKGYKVPTPIQRKVIPVILDGKDVVAMARTGSGKTACFLIPMFEKLKAHSAQTGVRGLILSPTRELALQTLKFTKELGKFTGLKTALILGGDKMEDQFAALHENPDIIIATPGRLMHVAIEMNLKLRTVEYVVFDEADRLFEMGFAEQLQEIISRLPETRQTLLFSATLPKMLLEFARAGLTEPVLIRLDVDTKLSDQLKLSFFNVRVEDKPAVLLHLLRCVVKPQEQTVIFVATKHHAEYLRELLDMQGIPCSHIYSALDQTARKINLGLFLHGKVRALLVTDVAARGIDIPMLDNVINYNFPPKAKLFLHRVGRVARAGRSGTAYSLIAPDEIPYVYDLHLFLGRPLKLATAQMMETGADTDGVLGRVPQSLIDDEDALLITDRERSLELQNLHNVAENAYKQYIKSRPTPSPESIKRVKDANYNLLGVHPLFWSQLGEEEMQRLKFVDGIKSYKSKATIFEINATNKTNASEVMRAKRSHDGKVIAKFQRLQEEKSATVQTLTSGISSLSCKQESDEENMEGVFSQVLGKRKITDKDEAEGNKKRKAGTSHDTDYYIPYRPKDFESERGLSIGASSFEQAAAGAVLDLLGDQTEDLRKNKQVMKWDRKKKKFVGTGGPEDKKKIKTESGRLISSSYKKNLYEGWKKKYKIDDQDSEEEDEERDQRAAAGRRKGRGGRGPQRSSHSSLGPQGKAKSELRSKQQILKTRKAQAKQNFLQKGGLKKMKNKTRHLANHMKKSAFGRGKSQGFKKGKSRK

Target ORF information:

RefSeq Version	XM_002934334.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAD-box helicase 54 (ddx54), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934334.4

ORF Insert Sequence:

ATGGCTCAGA AAAAACGTAA GGGACAGCGA TCTCGGAAAT GCTGTCAAAA CCAAGAGAGC 
GACGAAAGCG ACACAGTGGG ATTTCGTGTC AAAGCTGCAC CCCAAAAGGA GGATGGAGTA 
AAACTTCCAG CATTTCCCAC TGCAGATGCA ACTTCAGATA CAGAACTGGA CACCAGAGAA 
ATGGTGCGAG CACAGAATAA GAAGAAAAAG AAATCTGGCG GGTTTCAGTC CATGGGTCTT 
AGTTACCCAG TCTTTAAAGG AGTGATGAAG AAAGGTTACA AGGTTCCTAC TCCCATTCAG 
AGAAAGGTGA TTCCAGTCAT TCTTGATGGC AAGGATGTGG TAGCAATGGC TAGAACGGGC 
AGTGGAAAGA CTGCCTGTTT CCTTATCCCT ATGTTTGAGA AGTTAAAGGC TCACAGTGCC 
CAAACAGGAG TTCGTGGGCT TATCCTTTCA CCAACTAGAG AGCTGGCCTT GCAGACTCTG 
AAATTCACAA AGGAGCTTGG TAAATTCACT GGTCTGAAGA CTGCTCTGAT TTTAGGTGGT 
GACAAAATGG AAGATCAGTT TGCTGCTCTG CATGAAAATC CAGATATAAT TATTGCCACT 
CCTGGTCGAC TGATGCATGT GGCAATTGAG ATGAACCTGA AACTTCGCAC TGTGGAATAT 
GTGGTGTTTG ACGAAGCTGA CAGACTCTTT GAGATGGGAT TTGCAGAGCA GCTGCAAGAG 
ATAATCTCTC GACTACCAGA GACACGCCAG ACACTCCTTT TTTCCGCCAC TCTGCCCAAG 
ATGCTTCTAG AGTTTGCCCG AGCTGGCCTT ACTGAGCCTG TCTTAATACG TTTGGATGTT 
GATACAAAGC TGAGTGACCA GCTGAAGTTG TCCTTCTTCA ATGTGCGAGT AGAGGACAAA 
CCTGCTGTGC TTCTGCATCT CCTGCGCTGT GTTGTAAAGC CTCAAGAACA AACAGTCATT 
TTTGTTGCCA CTAAGCACCA TGCAGAATAC CTGAGAGAGT TGCTGGACAT GCAGGGAATT 
CCATGCTCTC ACATATACAG TGCTCTGGAT CAGACAGCCA GGAAGATAAA CCTGGGCCTT 
TTTTTGCATG GGAAGGTGCG TGCACTGCTT GTCACAGATG TGGCTGCTCG AGGTATTGAC 
ATACCAATGT TAGACAACGT CATAAACTAC AACTTCCCTC CGAAAGCCAA GCTTTTCCTG 
CATCGTGTTG GGCGTGTGGC AAGAGCAGGG CGAAGTGGGA CTGCTTACTC TCTTATTGCT 
CCTGATGAAA TTCCCTATGT GTATGATCTC CATCTCTTTC TTGGGCGGCC GCTTAAACTG 
GCAACAGCAC AAATGATGGA GACAGGAGCA GACACAGATG GGGTACTGGG GCGAGTACCA 
CAGAGTCTGA TTGATGATGA AGATGCCTTG CTTATTACGG ATCGTGAGCG ATCGCTGGAG 
CTGCAGAACT TGCACAACGT GGCAGAGAAT GCCTACAAAC AGTACATTAA ATCCAGGCCA 
ACTCCCTCTC CCGAGTCCAT CAAACGAGTC AAGGATGCTA ACTATAACCT GTTGGGGGTT 
CACCCACTCT TTTGGTCTCA GCTTGGTGAA GAAGAGATGC AGAGGCTGAA GTTTGTGGAT 
GGCATCAAAT CTTACAAATC TAAAGCAACC ATATTTGAAA TTAACGCTAC TAATAAAACC 
AATGCAAGTG AAGTTATGCG AGCCAAGAGA AGTCATGATG GGAAGGTCAT CGCTAAGTTC 
CAGCGCCTGC AGGAGGAGAA AAGTGCAACA GTGCAGACTT TGACCAGTGG CATCTCCAGC 
CTTAGCTGCA AACAAGAGAG TGATGAGGAG AACATGGAGG GAGTATTTTC GCAAGTGCTT 
GGAAAAAGGA AAATTACTGA CAAAGATGAA GCTGAAGGCA ACAAGAAAAG GAAAGCAGGA 
ACTTCTCATG ACACTGACTA TTACATCCCA TACAGACCAA AGGATTTTGA GAGTGAGAGG 
GGGCTCAGCA TTGGGGCCAG CAGCTTTGAG CAGGCAGCTG CAGGGGCTGT GTTGGACCTA 
TTGGGAGACC AGACGGAAGA TCTCAGGAAG AACAAACAAG TCATGAAGTG GGATAGAAAG 
AAAAAAAAGT TTGTGGGAAC AGGTGGACCA GAAGATAAAA AGAAAATTAA GACTGAGAGT 
GGACGACTCA TTAGTAGCTC CTATAAAAAA AACCTATATG AAGGCTGGAA GAAGAAATAC 
AAGATTGATG ACCAAGATTC TGAGGAAGAG GACGAGGAAA GAGATCAGAG GGCAGCTGCG 
GGGCGCAGGA AAGGGCGAGG TGGGCGTGGC CCCCAAAGAA GCTCTCATTC AAGTTTAGGG 
CCACAGGGGA AAGCCAAATC AGAGCTGCGT AGCAAACAGC AAATCCTCAA GACGAGAAAG 
GCACAAGCTA AACAGAACTT CTTGCAAAAA GGCGGTTTGA AGAAGATGAA AAACAAGACA 
AGACACCTAG CCAATCATAT GAAGAAATCT GCTTTTGGAA GAGGGAAAAG CCAAGGTTTC 
AAGAAAGGAA AATCAAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx54 ( XM_002934334.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934380.1 Xenopus tropicalis DEAD-box helicase 54 (ddx54), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa03708
Clone ID Related Accession (Same CDS sequence)	XM_002934334.4 , XM_002934334.5
Accession Version	XM_002934334.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-dependent RNA helicase DDX54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934334.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGA AAAAACGTAA GGGACAGCGA TCTCGGAAAT GCTGTCAAAA CCAAGAGAGC 
GACGAAAGCG ACACAGTGGG ATTTCGTGTC AAAGCTGCAC CCCAAAAGGA GGATGGAGTA 
AAACTTCCAG CATTTCCCAC TGCAGATGCA ACTTCAGATA CAGAACTGGA CACCAGAGAA 
ATGGTGCGAG CACAGAATAA GAAGAAAAAG AAATCTGGCG GGTTTCAGTC CATGGGTCTT 
AGTTACCCAG TCTTTAAAGG AGTGATGAAG AAAGGTTACA AGGTTCCTAC TCCCATTCAG 
AGAAAGGTGA TTCCAGTCAT TCTTGATGGC AAGGATGTGG TAGCAATGGC TAGAACGGGC 
AGTGGAAAGA CTGCCTGTTT CCTTATCCCT ATGTTTGAGA AGTTAAAGGC TCACAGTGCC 
CAAACAGGAG TTCGTGGGCT TATCCTTTCA CCAACTAGAG AGCTGGCCTT GCAGACTCTG 
AAATTCACAA AGGAGCTTGG TAAATTCACT GGTCTGAAGA CTGCTCTGAT TTTAGGTGGT 
GACAAAATGG AAGATCAGTT TGCTGCTCTG CATGAAAATC CAGATATAAT TATTGCCACT 
CCTGGTCGAC TGATGCATGT GGCAATTGAG ATGAACCTGA AACTTCGCAC TGTGGAATAT 
GTGGTGTTTG ACGAAGCTGA CAGACTCTTT GAGATGGGAT TTGCAGAGCA GCTGCAAGAG 
ATAATCTCTC GACTACCAGA GACACGCCAG ACACTCCTTT TTTCCGCCAC TCTGCCCAAG 
ATGCTTCTAG AGTTTGCCCG AGCTGGCCTT ACTGAGCCTG TCTTAATACG TTTGGATGTT 
GATACAAAGC TGAGTGACCA GCTGAAGTTG TCCTTCTTCA ATGTGCGAGT AGAGGACAAA 
CCTGCTGTGC TTCTGCATCT CCTGCGCTGT GTTGTAAAGC CTCAAGAACA AACAGTCATT 
TTTGTTGCCA CTAAGCACCA TGCAGAATAC CTGAGAGAGT TGCTGGACAT GCAGGGAATT 
CCATGCTCTC ACATATACAG TGCTCTGGAT CAGACAGCCA GGAAGATAAA CCTGGGCCTT 
TTTTTGCATG GGAAGGTGCG TGCACTGCTT GTCACAGATG TGGCTGCTCG AGGTATTGAC 
ATACCAATGT TAGACAACGT CATAAACTAC AACTTCCCTC CGAAAGCCAA GCTTTTCCTG 
CATCGTGTTG GGCGTGTGGC AAGAGCAGGG CGAAGTGGGA CTGCTTACTC TCTTATTGCT 
CCTGATGAAA TTCCCTATGT GTATGATCTC CATCTCTTTC TTGGGCGGCC GCTTAAACTG 
GCAACAGCAC AAATGATGGA GACAGGAGCA GACACAGATG GGGTACTGGG GCGAGTACCA 
CAGAGTCTGA TTGATGATGA AGATGCCTTG CTTATTACGG ATCGTGAGCG ATCGCTGGAG 
CTGCAGAACT TGCACAACGT GGCAGAGAAT GCCTACAAAC AGTACATTAA ATCCAGGCCA 
ACTCCCTCTC CCGAGTCCAT CAAACGAGTC AAGGATGCTA ACTATAACCT GTTGGGGGTT 
CACCCACTCT TTTGGTCTCA GCTTGGTGAA GAAGAGATGC AGAGGCTGAA GTTTGTGGAT 
GGCATCAAAT CTTACAAATC TAAAGCAACC ATATTTGAAA TTAACGCTAC TAATAAAACC 
AATGCAAGTG AAGTTATGCG AGCCAAGAGA AGTCATGATG GGAAGGTCAT CGCTAAGTTC 
CAGCGCCTGC AGGAGGAGAA AAGTGCAACA GTGCAGACTT TGACCAGTGG CATCTCCAGC 
CTTAGCTGCA AACAAGAGAG TGATGAGGAG AACATGGAGG GAGTATTTTC GCAAGTGCTT 
GGAAAAAGGA AAATTACTGA CAAAGATGAA GCTGAAGGCA ACAAGAAAAG GAAAGCAGGA 
ACTTCTCATG ACACTGACTA TTACATCCCA TACAGACCAA AGGATTTTGA GAGTGAGAGG 
GGGCTCAGCA TTGGGGCCAG CAGCTTTGAG CAGGCAGCTG CAGGGGCTGT GTTGGACCTA 
TTGGGAGACC AGACGGAAGA TCTCAGGAAG AACAAACAAG TCATGAAGTG GGATAGAAAG 
AAAAAAAAGT TTGTGGGAAC AGGTGGACCA GAAGATAAAA AGAAAATTAA GACTGAGAGT 
GGACGACTCA TTAGTAGCTC CTATAAAAAA AACCTATATG AAGGCTGGAA GAAGAAATAC 
AAGATTGATG ACCAAGATTC TGAGGAAGAG GACGAGGAAA GAGATCAGAG GGCAGCTGCG 
GGGCGCAGGA AAGGGCGAGG TGGGCGTGGC CCCCAAAGAA GCTCTCATTC AAGTTTAGGG 
CCACAGGGGA AAGCCAAATC AGAGCTGCGT AGCAAACAGC AAATCCTCAA GACGAGAAAG 
GCACAAGCTA AACAGAACTT CTTGCAAAAA GGCGGTTTGA AGAAGATGAA AAACAAGACA 
AGACACCTAG CCAATCATAT GAAGAAATCT GCTTTTGGAA GAGGGAAAAG CCAAGGTTTC 
AAGAAAGGAA AATCAAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934380.1
CDS	140..2683
Translation	MAQKKRKGQRSRKCCQNQESDESDTVGFRVKAAPQKEDGVKLPAFPTADATSDTELDTREMVRAQNKKKKKSGGFQSMGLSYPVFKGVMKKGYKVPTPIQRKVIPVILDGKDVVAMARTGSGKTACFLIPMFEKLKAHSAQTGVRGLILSPTRELALQTLKFTKELGKFTGLKTALILGGDKMEDQFAALHENPDIIIATPGRLMHVAIEMNLKLRTVEYVVFDEADRLFEMGFAEQLQEIISRLPETRQTLLFSATLPKMLLEFARAGLTEPVLIRLDVDTKLSDQLKLSFFNVRVEDKPAVLLHLLRCVVKPQEQTVIFVATKHHAEYLRELLDMQGIPCSHIYSALDQTARKINLGLFLHGKVRALLVTDVAARGIDIPMLDNVINYNFPPKAKLFLHRVGRVARAGRSGTAYSLIAPDEIPYVYDLHLFLGRPLKLATAQMMETGADTDGVLGRVPQSLIDDEDALLITDRERSLELQNLHNVAENAYKQYIKSRPTPSPESIKRVKDANYNLLGVHPLFWSQLGEEEMQRLKFVDGIKSYKSKATIFEINATNKTNASEVMRAKRSHDGKVIAKFQRLQEEKSATVQTLTSGISSLSCKQESDEENMEGVFSQVLGKRKITDKDEAEGNKKRKAGTSHDTDYYIPYRPKDFESERGLSIGASSFEQAAAGAVLDLLGDQTEDLRKNKQVMKWDRKKKKFVGTGGPEDKKKIKTESGRLISSSYKKNLYEGWKKKYKIDDQDSEEEDEERDQRAAAGRRKGRGGRGPQRSSHSSLGPQGKAKSELRSKQQILKTRKAQAKQNFLQKGGLKKMKNKTRHLANHMKKSAFGRGKSQGFKKGKSRK

Target ORF information:

RefSeq Version	XM_002934334.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAD-box helicase 54 (ddx54), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934334.5

ORF Insert Sequence:

ATGGCTCAGA AAAAACGTAA GGGACAGCGA TCTCGGAAAT GCTGTCAAAA CCAAGAGAGC 
GACGAAAGCG ACACAGTGGG ATTTCGTGTC AAAGCTGCAC CCCAAAAGGA GGATGGAGTA 
AAACTTCCAG CATTTCCCAC TGCAGATGCA ACTTCAGATA CAGAACTGGA CACCAGAGAA 
ATGGTGCGAG CACAGAATAA GAAGAAAAAG AAATCTGGCG GGTTTCAGTC CATGGGTCTT 
AGTTACCCAG TCTTTAAAGG AGTGATGAAG AAAGGTTACA AGGTTCCTAC TCCCATTCAG 
AGAAAGGTGA TTCCAGTCAT TCTTGATGGC AAGGATGTGG TAGCAATGGC TAGAACGGGC 
AGTGGAAAGA CTGCCTGTTT CCTTATCCCT ATGTTTGAGA AGTTAAAGGC TCACAGTGCC 
CAAACAGGAG TTCGTGGGCT TATCCTTTCA CCAACTAGAG AGCTGGCCTT GCAGACTCTG 
AAATTCACAA AGGAGCTTGG TAAATTCACT GGTCTGAAGA CTGCTCTGAT TTTAGGTGGT 
GACAAAATGG AAGATCAGTT TGCTGCTCTG CATGAAAATC CAGATATAAT TATTGCCACT 
CCTGGTCGAC TGATGCATGT GGCAATTGAG ATGAACCTGA AACTTCGCAC TGTGGAATAT 
GTGGTGTTTG ACGAAGCTGA CAGACTCTTT GAGATGGGAT TTGCAGAGCA GCTGCAAGAG 
ATAATCTCTC GACTACCAGA GACACGCCAG ACACTCCTTT TTTCCGCCAC TCTGCCCAAG 
ATGCTTCTAG AGTTTGCCCG AGCTGGCCTT ACTGAGCCTG TCTTAATACG TTTGGATGTT 
GATACAAAGC TGAGTGACCA GCTGAAGTTG TCCTTCTTCA ATGTGCGAGT AGAGGACAAA 
CCTGCTGTGC TTCTGCATCT CCTGCGCTGT GTTGTAAAGC CTCAAGAACA AACAGTCATT 
TTTGTTGCCA CTAAGCACCA TGCAGAATAC CTGAGAGAGT TGCTGGACAT GCAGGGAATT 
CCATGCTCTC ACATATACAG TGCTCTGGAT CAGACAGCCA GGAAGATAAA CCTGGGCCTT 
TTTTTGCATG GGAAGGTGCG TGCACTGCTT GTCACAGATG TGGCTGCTCG AGGTATTGAC 
ATACCAATGT TAGACAACGT CATAAACTAC AACTTCCCTC CGAAAGCCAA GCTTTTCCTG 
CATCGTGTTG GGCGTGTGGC AAGAGCAGGG CGAAGTGGGA CTGCTTACTC TCTTATTGCT 
CCTGATGAAA TTCCCTATGT GTATGATCTC CATCTCTTTC TTGGGCGGCC GCTTAAACTG 
GCAACAGCAC AAATGATGGA GACAGGAGCA GACACAGATG GGGTACTGGG GCGAGTACCA 
CAGAGTCTGA TTGATGATGA AGATGCCTTG CTTATTACGG ATCGTGAGCG ATCGCTGGAG 
CTGCAGAACT TGCACAACGT GGCAGAGAAT GCCTACAAAC AGTACATTAA ATCCAGGCCA 
ACTCCCTCTC CCGAGTCCAT CAAACGAGTC AAGGATGCTA ACTATAACCT GTTGGGGGTT 
CACCCACTCT TTTGGTCTCA GCTTGGTGAA GAAGAGATGC AGAGGCTGAA GTTTGTGGAT 
GGCATCAAAT CTTACAAATC TAAAGCAACC ATATTTGAAA TTAACGCTAC TAATAAAACC 
AATGCAAGTG AAGTTATGCG AGCCAAGAGA AGTCATGATG GGAAGGTCAT CGCTAAGTTC 
CAGCGCCTGC AGGAGGAGAA AAGTGCAACA GTGCAGACTT TGACCAGTGG CATCTCCAGC 
CTTAGCTGCA AACAAGAGAG TGATGAGGAG AACATGGAGG GAGTATTTTC GCAAGTGCTT 
GGAAAAAGGA AAATTACTGA CAAAGATGAA GCTGAAGGCA ACAAGAAAAG GAAAGCAGGA 
ACTTCTCATG ACACTGACTA TTACATCCCA TACAGACCAA AGGATTTTGA GAGTGAGAGG 
GGGCTCAGCA TTGGGGCCAG CAGCTTTGAG CAGGCAGCTG CAGGGGCTGT GTTGGACCTA 
TTGGGAGACC AGACGGAAGA TCTCAGGAAG AACAAACAAG TCATGAAGTG GGATAGAAAG 
AAAAAAAAGT TTGTGGGAAC AGGTGGACCA GAAGATAAAA AGAAAATTAA GACTGAGAGT 
GGACGACTCA TTAGTAGCTC CTATAAAAAA AACCTATATG AAGGCTGGAA GAAGAAATAC 
AAGATTGATG ACCAAGATTC TGAGGAAGAG GACGAGGAAA GAGATCAGAG GGCAGCTGCG 
GGGCGCAGGA AAGGGCGAGG TGGGCGTGGC CCCCAAAGAA GCTCTCATTC AAGTTTAGGG 
CCACAGGGGA AAGCCAAATC AGAGCTGCGT AGCAAACAGC AAATCCTCAA GACGAGAAAG 
GCACAAGCTA AACAGAACTT CTTGCAAAAA GGCGGTTTGA AGAAGATGAA AAACAAGACA 
AGACACCTAG CCAATCATAT GAAGAAATCT GCTTTTGGAA GAGGGAAAAG CCAAGGTTTC 
AAGAAAGGAA AATCAAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx54 ( XM_018089863.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945352.1 Xenopus tropicalis DEAD-box helicase 54 (ddx54), transcript variant X2, mRNA.

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CloneID	OXa42139
Clone ID Related Accession (Same CDS sequence)	XM_018089863.1 , XM_018089863.2
Accession Version	XM_018089863.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-dependent RNA helicase DDX54 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCT GTTCTAGAAA ATGTGAAAAA CTTCCAGCAT TTCCCACTGC AGATGCAACT 
TCAGATACAG AACTGGACAC CAGAGAAATG GTGCGAGCAC AGAATAAGAA GAAAAAGAAA 
TCTGGCGGGT TTCAGTCCAT GGGTCTTAGT TACCCAGTCT TTAAAGGAGT GATGAAGAAA 
GGTTACAAGG TTCCTACTCC CATTCAGAGA AAGGTGATTC CAGTCATTCT TGATGGCAAG 
GATGTGGTAG CAATGGCTAG AACGGGCAGT GGAAAGACTG CCTGTTTCCT TATCCCTATG 
TTTGAGAAGT TAAAGGCTCA CAGTGCCCAA ACAGGAGTTC GTGGGCTTAT CCTTTCACCA 
ACTAGAGAGC TGGCCTTGCA GACTCTGAAA TTCACAAAGG AGCTTGGTAA ATTCACTGGT 
CTGAAGACTG CTCTGATTTT AGGTGGTGAC AAAATGGAAG ATCAGTTTGC TGCTCTGCAT 
GAAAATCCAG ATATAATTAT TGCCACTCCT GGTCGACTGA TGCATGTGGC AATTGAGATG 
AACCTGAAAC TTCGCACTGT GGAATATGTG GTGTTTGACG AAGCTGACAG ACTCTTTGAG 
ATGGGATTTG CAGAGCAGCT GCAAGAGATA ATCTCTCGAC TACCAGAGAC ACGCCAGACA 
CTCCTTTTTT CCGCCACTCT GCCCAAGATG CTTCTAGAGT TTGCCCGAGC TGGCCTTACT 
GAGCCTGTCT TAATACGTTT GGATGTTGAT ACAAAGCTGA GTGACCAGCT GAAGTTGTCC 
TTCTTCAATG TGCGAGTAGA GGACAAACCT GCTGTGCTTC TGCATCTCCT GCGCTGTGTT 
GTAAAGCCTC AAGAACAAAC AGTCATTTTT GTTGCCACTA AGCACCATGC AGAATACCTG 
AGAGAGTTGC TGGACATGCA GGGAATTCCA TGCTCTCACA TATACAGTGC TCTGGATCAG 
ACAGCCAGGA AGATAAACCT GGGCCTTTTT TTGCATGGGA AGGTGCGTGC ACTGCTTGTC 
ACAGATGTGG CTGCTCGAGG TATTGACATA CCAATGTTAG ACAACGTCAT AAACTACAAC 
TTCCCTCCGA AAGCCAAGCT TTTCCTGCAT CGTGTTGGGC GTGTGGCAAG AGCAGGGCGA 
AGTGGGACTG CTTACTCTCT TATTGCTCCT GATGAAATTC CCTATGTGTA TGATCTCCAT 
CTCTTTCTTG GGCGGCCGCT TAAACTGGCA ACAGCACAAA TGATGGAGAC AGGAGCAGAC 
ACAGATGGGG TACTGGGGCG AGTACCACAG AGTCTGATTG ATGATGAAGA TGCCTTGCTT 
ATTACGGATC GTGAGCGATC GCTGGAGCTG CAGAACTTGC ACAACGTGGC AGAGAATGCC 
TACAAACAGT ACATTAAATC CAGGCCAACT CCCTCTCCCG AGTCCATCAA ACGAGTCAAG 
GATGCTAACT ATAACCTGTT GGGGGTTCAC CCACTCTTTT GGTCTCAGCT TGGTGAAGAA 
GAGATGCAGA GGCTGAAGTT TGTGGATGGC ATCAAATCTT ACAAATCTAA AGCAACCATA 
TTTGAAATTA ACGCTACTAA TAAAACCAAT GCAAGTGAAG TTATGCGAGC CAAGAGAAGT 
CATGATGGGA AGGTCATCGC TAAGTTCCAG CGCCTGCAGG AGGAGAAAAG TGCAACAGTG 
CAGACTTTGA CCAGTGGCAT CTCCAGCCTT AGCTGCAAAC AAGAGAGTGA TGAGGAGAAC 
ATGGAGGGAG TATTTTCGCA AGTGCTTGGA AAAAGGAAAA TTACTGACAA AGATGAAGCT 
GAAGGCAACA AGAAAAGGAA AGCAGGAACT TCTCATGACA CTGACTATTA CATCCCATAC 
AGACCAAAGG ATTTTGAGAG TGAGAGGGGG CTCAGCATTG GGGCCAGCAG CTTTGAGCAG 
GCAGCTGCAG GGGCTGTGTT GGACCTATTG GGAGACCAGA CGGAAGATCT CAGGAAGAAC 
AAACAAGTCA TGAAGTGGGA TAGAAAGAAA AAAAAGTTTG TGGGAACAGG TGGACCAGAA 
GATAAAAAGA AAATTAAGAC TGAGAGTGGA CGACTCATTA GTAGCTCCTA TAAAAAAAAC 
CTATATGAAG GCTGGAAGAA GAAATACAAG ATTGATGACC AAGATTCTGA GGAAGAGGAC 
GAGGAAAGAG ATCAGAGGGC AGCTGCGGGG CGCAGGAAAG GGCGAGGTGG GCGTGGCCCC 
CAAAGAAGCT CTCATTCAAG TTTAGGGCCA CAGGGGAAAG CCAAATCAGA GCTGCGTAGC 
AAACAGCAAA TCCTCAAGAC GAGAAAGGCA CAAGCTAAAC AGAACTTCTT GCAAAAAGGC 
GGTTTGAAGA AGATGAAAAA CAAGACAAGA CACCTAGCCA ATCATATGAA GAAATCTGCT 
TTTGGAAGAG GGAAAAGCCA AGGTTTCAAG AAAGGAAAAT CAAGAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945352.1
CDS	6..2456
Translation	MAACSRKCEKLPAFPTADATSDTELDTREMVRAQNKKKKKSGGFQSMGLSYPVFKGVMKKGYKVPTPIQRKVIPVILDGKDVVAMARTGSGKTACFLIPMFEKLKAHSAQTGVRGLILSPTRELALQTLKFTKELGKFTGLKTALILGGDKMEDQFAALHENPDIIIATPGRLMHVAIEMNLKLRTVEYVVFDEADRLFEMGFAEQLQEIISRLPETRQTLLFSATLPKMLLEFARAGLTEPVLIRLDVDTKLSDQLKLSFFNVRVEDKPAVLLHLLRCVVKPQEQTVIFVATKHHAEYLRELLDMQGIPCSHIYSALDQTARKINLGLFLHGKVRALLVTDVAARGIDIPMLDNVINYNFPPKAKLFLHRVGRVARAGRSGTAYSLIAPDEIPYVYDLHLFLGRPLKLATAQMMETGADTDGVLGRVPQSLIDDEDALLITDRERSLELQNLHNVAENAYKQYIKSRPTPSPESIKRVKDANYNLLGVHPLFWSQLGEEEMQRLKFVDGIKSYKSKATIFEINATNKTNASEVMRAKRSHDGKVIAKFQRLQEEKSATVQTLTSGISSLSCKQESDEENMEGVFSQVLGKRKITDKDEAEGNKKRKAGTSHDTDYYIPYRPKDFESERGLSIGASSFEQAAAGAVLDLLGDQTEDLRKNKQVMKWDRKKKKFVGTGGPEDKKKIKTESGRLISSSYKKNLYEGWKKKYKIDDQDSEEEDEERDQRAAAGRRKGRGGRGPQRSSHSSLGPQGKAKSELRSKQQILKTRKAQAKQNFLQKGGLKKMKNKTRHLANHMKKSAFGRGKSQGFKKGKSRK

Target ORF information:

RefSeq Version	XM_018089863.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAD-box helicase 54 (ddx54), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089863.1

ORF Insert Sequence:

ATGGCTGCCT GTTCTAGAAA ATGTGAAAAA CTTCCAGCAT TTCCCACTGC AGATGCAACT 
TCAGATACAG AACTGGACAC CAGAGAAATG GTGCGAGCAC AGAATAAGAA GAAAAAGAAA 
TCTGGCGGGT TTCAGTCCAT GGGTCTTAGT TACCCAGTCT TTAAAGGAGT GATGAAGAAA 
GGTTACAAGG TTCCTACTCC CATTCAGAGA AAGGTGATTC CAGTCATTCT TGATGGCAAG 
GATGTGGTAG CAATGGCTAG AACGGGCAGT GGAAAGACTG CCTGTTTCCT TATCCCTATG 
TTTGAGAAGT TAAAGGCTCA CAGTGCCCAA ACAGGAGTTC GTGGGCTTAT CCTTTCACCA 
ACTAGAGAGC TGGCCTTGCA GACTCTGAAA TTCACAAAGG AGCTTGGTAA ATTCACTGGT 
CTGAAGACTG CTCTGATTTT AGGTGGTGAC AAAATGGAAG ATCAGTTTGC TGCTCTGCAT 
GAAAATCCAG ATATAATTAT TGCCACTCCT GGTCGACTGA TGCATGTGGC AATTGAGATG 
AACCTGAAAC TTCGCACTGT GGAATATGTG GTGTTTGACG AAGCTGACAG ACTCTTTGAG 
ATGGGATTTG CAGAGCAGCT GCAAGAGATA ATCTCTCGAC TACCAGAGAC ACGCCAGACA 
CTCCTTTTTT CCGCCACTCT GCCCAAGATG CTTCTAGAGT TTGCCCGAGC TGGCCTTACT 
GAGCCTGTCT TAATACGTTT GGATGTTGAT ACAAAGCTGA GTGACCAGCT GAAGTTGTCC 
TTCTTCAATG TGCGAGTAGA GGACAAACCT GCTGTGCTTC TGCATCTCCT GCGCTGTGTT 
GTAAAGCCTC AAGAACAAAC AGTCATTTTT GTTGCCACTA AGCACCATGC AGAATACCTG 
AGAGAGTTGC TGGACATGCA GGGAATTCCA TGCTCTCACA TATACAGTGC TCTGGATCAG 
ACAGCCAGGA AGATAAACCT GGGCCTTTTT TTGCATGGGA AGGTGCGTGC ACTGCTTGTC 
ACAGATGTGG CTGCTCGAGG TATTGACATA CCAATGTTAG ACAACGTCAT AAACTACAAC 
TTCCCTCCGA AAGCCAAGCT TTTCCTGCAT CGTGTTGGGC GTGTGGCAAG AGCAGGGCGA 
AGTGGGACTG CTTACTCTCT TATTGCTCCT GATGAAATTC CCTATGTGTA TGATCTCCAT 
CTCTTTCTTG GGCGGCCGCT TAAACTGGCA ACAGCACAAA TGATGGAGAC AGGAGCAGAC 
ACAGATGGGG TACTGGGGCG AGTACCACAG AGTCTGATTG ATGATGAAGA TGCCTTGCTT 
ATTACGGATC GTGAGCGATC GCTGGAGCTG CAGAACTTGC ACAACGTGGC AGAGAATGCC 
TACAAACAGT ACATTAAATC CAGGCCAACT CCCTCTCCCG AGTCCATCAA ACGAGTCAAG 
GATGCTAACT ATAACCTGTT GGGGGTTCAC CCACTCTTTT GGTCTCAGCT TGGTGAAGAA 
GAGATGCAGA GGCTGAAGTT TGTGGATGGC ATCAAATCTT ACAAATCTAA AGCAACCATA 
TTTGAAATTA ACGCTACTAA TAAAACCAAT GCAAGTGAAG TTATGCGAGC CAAGAGAAGT 
CATGATGGGA AGGTCATCGC TAAGTTCCAG CGCCTGCAGG AGGAGAAAAG TGCAACAGTG 
CAGACTTTGA CCAGTGGCAT CTCCAGCCTT AGCTGCAAAC AAGAGAGTGA TGAGGAGAAC 
ATGGAGGGAG TATTTTCGCA AGTGCTTGGA AAAAGGAAAA TTACTGACAA AGATGAAGCT 
GAAGGCAACA AGAAAAGGAA AGCAGGAACT TCTCATGACA CTGACTATTA CATCCCATAC 
AGACCAAAGG ATTTTGAGAG TGAGAGGGGG CTCAGCATTG GGGCCAGCAG CTTTGAGCAG 
GCAGCTGCAG GGGCTGTGTT GGACCTATTG GGAGACCAGA CGGAAGATCT CAGGAAGAAC 
AAACAAGTCA TGAAGTGGGA TAGAAAGAAA AAAAAGTTTG TGGGAACAGG TGGACCAGAA 
GATAAAAAGA AAATTAAGAC TGAGAGTGGA CGACTCATTA GTAGCTCCTA TAAAAAAAAC 
CTATATGAAG GCTGGAAGAA GAAATACAAG ATTGATGACC AAGATTCTGA GGAAGAGGAC 
GAGGAAAGAG ATCAGAGGGC AGCTGCGGGG CGCAGGAAAG GGCGAGGTGG GCGTGGCCCC 
CAAAGAAGCT CTCATTCAAG TTTAGGGCCA CAGGGGAAAG CCAAATCAGA GCTGCGTAGC 
AAACAGCAAA TCCTCAAGAC GAGAAAGGCA CAAGCTAAAC AGAACTTCTT GCAAAAAGGC 
GGTTTGAAGA AGATGAAAAA CAAGACAAGA CACCTAGCCA ATCATATGAA GAAATCTGCT 
TTTGGAAGAG GGAAAAGCCA AGGTTTCAAG AAAGGAAAAT CAAGAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx54 ( XM_018089863.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945352.1 Xenopus tropicalis DEAD-box helicase 54 (ddx54), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa42139
Clone ID Related Accession (Same CDS sequence)	XM_018089863.1 , XM_018089863.2
Accession Version	XM_018089863.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-dependent RNA helicase DDX54 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018089863.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCT GTTCTAGAAA ATGTGAAAAA CTTCCAGCAT TTCCCACTGC AGATGCAACT 
TCAGATACAG AACTGGACAC CAGAGAAATG GTGCGAGCAC AGAATAAGAA GAAAAAGAAA 
TCTGGCGGGT TTCAGTCCAT GGGTCTTAGT TACCCAGTCT TTAAAGGAGT GATGAAGAAA 
GGTTACAAGG TTCCTACTCC CATTCAGAGA AAGGTGATTC CAGTCATTCT TGATGGCAAG 
GATGTGGTAG CAATGGCTAG AACGGGCAGT GGAAAGACTG CCTGTTTCCT TATCCCTATG 
TTTGAGAAGT TAAAGGCTCA CAGTGCCCAA ACAGGAGTTC GTGGGCTTAT CCTTTCACCA 
ACTAGAGAGC TGGCCTTGCA GACTCTGAAA TTCACAAAGG AGCTTGGTAA ATTCACTGGT 
CTGAAGACTG CTCTGATTTT AGGTGGTGAC AAAATGGAAG ATCAGTTTGC TGCTCTGCAT 
GAAAATCCAG ATATAATTAT TGCCACTCCT GGTCGACTGA TGCATGTGGC AATTGAGATG 
AACCTGAAAC TTCGCACTGT GGAATATGTG GTGTTTGACG AAGCTGACAG ACTCTTTGAG 
ATGGGATTTG CAGAGCAGCT GCAAGAGATA ATCTCTCGAC TACCAGAGAC ACGCCAGACA 
CTCCTTTTTT CCGCCACTCT GCCCAAGATG CTTCTAGAGT TTGCCCGAGC TGGCCTTACT 
GAGCCTGTCT TAATACGTTT GGATGTTGAT ACAAAGCTGA GTGACCAGCT GAAGTTGTCC 
TTCTTCAATG TGCGAGTAGA GGACAAACCT GCTGTGCTTC TGCATCTCCT GCGCTGTGTT 
GTAAAGCCTC AAGAACAAAC AGTCATTTTT GTTGCCACTA AGCACCATGC AGAATACCTG 
AGAGAGTTGC TGGACATGCA GGGAATTCCA TGCTCTCACA TATACAGTGC TCTGGATCAG 
ACAGCCAGGA AGATAAACCT GGGCCTTTTT TTGCATGGGA AGGTGCGTGC ACTGCTTGTC 
ACAGATGTGG CTGCTCGAGG TATTGACATA CCAATGTTAG ACAACGTCAT AAACTACAAC 
TTCCCTCCGA AAGCCAAGCT TTTCCTGCAT CGTGTTGGGC GTGTGGCAAG AGCAGGGCGA 
AGTGGGACTG CTTACTCTCT TATTGCTCCT GATGAAATTC CCTATGTGTA TGATCTCCAT 
CTCTTTCTTG GGCGGCCGCT TAAACTGGCA ACAGCACAAA TGATGGAGAC AGGAGCAGAC 
ACAGATGGGG TACTGGGGCG AGTACCACAG AGTCTGATTG ATGATGAAGA TGCCTTGCTT 
ATTACGGATC GTGAGCGATC GCTGGAGCTG CAGAACTTGC ACAACGTGGC AGAGAATGCC 
TACAAACAGT ACATTAAATC CAGGCCAACT CCCTCTCCCG AGTCCATCAA ACGAGTCAAG 
GATGCTAACT ATAACCTGTT GGGGGTTCAC CCACTCTTTT GGTCTCAGCT TGGTGAAGAA 
GAGATGCAGA GGCTGAAGTT TGTGGATGGC ATCAAATCTT ACAAATCTAA AGCAACCATA 
TTTGAAATTA ACGCTACTAA TAAAACCAAT GCAAGTGAAG TTATGCGAGC CAAGAGAAGT 
CATGATGGGA AGGTCATCGC TAAGTTCCAG CGCCTGCAGG AGGAGAAAAG TGCAACAGTG 
CAGACTTTGA CCAGTGGCAT CTCCAGCCTT AGCTGCAAAC AAGAGAGTGA TGAGGAGAAC 
ATGGAGGGAG TATTTTCGCA AGTGCTTGGA AAAAGGAAAA TTACTGACAA AGATGAAGCT 
GAAGGCAACA AGAAAAGGAA AGCAGGAACT TCTCATGACA CTGACTATTA CATCCCATAC 
AGACCAAAGG ATTTTGAGAG TGAGAGGGGG CTCAGCATTG GGGCCAGCAG CTTTGAGCAG 
GCAGCTGCAG GGGCTGTGTT GGACCTATTG GGAGACCAGA CGGAAGATCT CAGGAAGAAC 
AAACAAGTCA TGAAGTGGGA TAGAAAGAAA AAAAAGTTTG TGGGAACAGG TGGACCAGAA 
GATAAAAAGA AAATTAAGAC TGAGAGTGGA CGACTCATTA GTAGCTCCTA TAAAAAAAAC 
CTATATGAAG GCTGGAAGAA GAAATACAAG ATTGATGACC AAGATTCTGA GGAAGAGGAC 
GAGGAAAGAG ATCAGAGGGC AGCTGCGGGG CGCAGGAAAG GGCGAGGTGG GCGTGGCCCC 
CAAAGAAGCT CTCATTCAAG TTTAGGGCCA CAGGGGAAAG CCAAATCAGA GCTGCGTAGC 
AAACAGCAAA TCCTCAAGAC GAGAAAGGCA CAAGCTAAAC AGAACTTCTT GCAAAAAGGC 
GGTTTGAAGA AGATGAAAAA CAAGACAAGA CACCTAGCCA ATCATATGAA GAAATCTGCT 
TTTGGAAGAG GGAAAAGCCA AGGTTTCAAG AAAGGAAAAT CAAGAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945352.1
CDS	6..2456
Translation	MAACSRKCEKLPAFPTADATSDTELDTREMVRAQNKKKKKSGGFQSMGLSYPVFKGVMKKGYKVPTPIQRKVIPVILDGKDVVAMARTGSGKTACFLIPMFEKLKAHSAQTGVRGLILSPTRELALQTLKFTKELGKFTGLKTALILGGDKMEDQFAALHENPDIIIATPGRLMHVAIEMNLKLRTVEYVVFDEADRLFEMGFAEQLQEIISRLPETRQTLLFSATLPKMLLEFARAGLTEPVLIRLDVDTKLSDQLKLSFFNVRVEDKPAVLLHLLRCVVKPQEQTVIFVATKHHAEYLRELLDMQGIPCSHIYSALDQTARKINLGLFLHGKVRALLVTDVAARGIDIPMLDNVINYNFPPKAKLFLHRVGRVARAGRSGTAYSLIAPDEIPYVYDLHLFLGRPLKLATAQMMETGADTDGVLGRVPQSLIDDEDALLITDRERSLELQNLHNVAENAYKQYIKSRPTPSPESIKRVKDANYNLLGVHPLFWSQLGEEEMQRLKFVDGIKSYKSKATIFEINATNKTNASEVMRAKRSHDGKVIAKFQRLQEEKSATVQTLTSGISSLSCKQESDEENMEGVFSQVLGKRKITDKDEAEGNKKRKAGTSHDTDYYIPYRPKDFESERGLSIGASSFEQAAAGAVLDLLGDQTEDLRKNKQVMKWDRKKKKFVGTGGPEDKKKIKTESGRLISSSYKKNLYEGWKKKYKIDDQDSEEEDEERDQRAAAGRRKGRGGRGPQRSSHSSLGPQGKAKSELRSKQQILKTRKAQAKQNFLQKGGLKKMKNKTRHLANHMKKSAFGRGKSQGFKKGKSRK

Target ORF information:

RefSeq Version	XM_018089863.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAD-box helicase 54 (ddx54), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089863.2

ORF Insert Sequence:

ATGGCTGCCT GTTCTAGAAA ATGTGAAAAA CTTCCAGCAT TTCCCACTGC AGATGCAACT 
TCAGATACAG AACTGGACAC CAGAGAAATG GTGCGAGCAC AGAATAAGAA GAAAAAGAAA 
TCTGGCGGGT TTCAGTCCAT GGGTCTTAGT TACCCAGTCT TTAAAGGAGT GATGAAGAAA 
GGTTACAAGG TTCCTACTCC CATTCAGAGA AAGGTGATTC CAGTCATTCT TGATGGCAAG 
GATGTGGTAG CAATGGCTAG AACGGGCAGT GGAAAGACTG CCTGTTTCCT TATCCCTATG 
TTTGAGAAGT TAAAGGCTCA CAGTGCCCAA ACAGGAGTTC GTGGGCTTAT CCTTTCACCA 
ACTAGAGAGC TGGCCTTGCA GACTCTGAAA TTCACAAAGG AGCTTGGTAA ATTCACTGGT 
CTGAAGACTG CTCTGATTTT AGGTGGTGAC AAAATGGAAG ATCAGTTTGC TGCTCTGCAT 
GAAAATCCAG ATATAATTAT TGCCACTCCT GGTCGACTGA TGCATGTGGC AATTGAGATG 
AACCTGAAAC TTCGCACTGT GGAATATGTG GTGTTTGACG AAGCTGACAG ACTCTTTGAG 
ATGGGATTTG CAGAGCAGCT GCAAGAGATA ATCTCTCGAC TACCAGAGAC ACGCCAGACA 
CTCCTTTTTT CCGCCACTCT GCCCAAGATG CTTCTAGAGT TTGCCCGAGC TGGCCTTACT 
GAGCCTGTCT TAATACGTTT GGATGTTGAT ACAAAGCTGA GTGACCAGCT GAAGTTGTCC 
TTCTTCAATG TGCGAGTAGA GGACAAACCT GCTGTGCTTC TGCATCTCCT GCGCTGTGTT 
GTAAAGCCTC AAGAACAAAC AGTCATTTTT GTTGCCACTA AGCACCATGC AGAATACCTG 
AGAGAGTTGC TGGACATGCA GGGAATTCCA TGCTCTCACA TATACAGTGC TCTGGATCAG 
ACAGCCAGGA AGATAAACCT GGGCCTTTTT TTGCATGGGA AGGTGCGTGC ACTGCTTGTC 
ACAGATGTGG CTGCTCGAGG TATTGACATA CCAATGTTAG ACAACGTCAT AAACTACAAC 
TTCCCTCCGA AAGCCAAGCT TTTCCTGCAT CGTGTTGGGC GTGTGGCAAG AGCAGGGCGA 
AGTGGGACTG CTTACTCTCT TATTGCTCCT GATGAAATTC CCTATGTGTA TGATCTCCAT 
CTCTTTCTTG GGCGGCCGCT TAAACTGGCA ACAGCACAAA TGATGGAGAC AGGAGCAGAC 
ACAGATGGGG TACTGGGGCG AGTACCACAG AGTCTGATTG ATGATGAAGA TGCCTTGCTT 
ATTACGGATC GTGAGCGATC GCTGGAGCTG CAGAACTTGC ACAACGTGGC AGAGAATGCC 
TACAAACAGT ACATTAAATC CAGGCCAACT CCCTCTCCCG AGTCCATCAA ACGAGTCAAG 
GATGCTAACT ATAACCTGTT GGGGGTTCAC CCACTCTTTT GGTCTCAGCT TGGTGAAGAA 
GAGATGCAGA GGCTGAAGTT TGTGGATGGC ATCAAATCTT ACAAATCTAA AGCAACCATA 
TTTGAAATTA ACGCTACTAA TAAAACCAAT GCAAGTGAAG TTATGCGAGC CAAGAGAAGT 
CATGATGGGA AGGTCATCGC TAAGTTCCAG CGCCTGCAGG AGGAGAAAAG TGCAACAGTG 
CAGACTTTGA CCAGTGGCAT CTCCAGCCTT AGCTGCAAAC AAGAGAGTGA TGAGGAGAAC 
ATGGAGGGAG TATTTTCGCA AGTGCTTGGA AAAAGGAAAA TTACTGACAA AGATGAAGCT 
GAAGGCAACA AGAAAAGGAA AGCAGGAACT TCTCATGACA CTGACTATTA CATCCCATAC 
AGACCAAAGG ATTTTGAGAG TGAGAGGGGG CTCAGCATTG GGGCCAGCAG CTTTGAGCAG 
GCAGCTGCAG GGGCTGTGTT GGACCTATTG GGAGACCAGA CGGAAGATCT CAGGAAGAAC 
AAACAAGTCA TGAAGTGGGA TAGAAAGAAA AAAAAGTTTG TGGGAACAGG TGGACCAGAA 
GATAAAAAGA AAATTAAGAC TGAGAGTGGA CGACTCATTA GTAGCTCCTA TAAAAAAAAC 
CTATATGAAG GCTGGAAGAA GAAATACAAG ATTGATGACC AAGATTCTGA GGAAGAGGAC 
GAGGAAAGAG ATCAGAGGGC AGCTGCGGGG CGCAGGAAAG GGCGAGGTGG GCGTGGCCCC 
CAAAGAAGCT CTCATTCAAG TTTAGGGCCA CAGGGGAAAG CCAAATCAGA GCTGCGTAGC 
AAACAGCAAA TCCTCAAGAC GAGAAAGGCA CAAGCTAAAC AGAACTTCTT GCAAAAAGGC 
GGTTTGAAGA AGATGAAAAA CAAGACAAGA CACCTAGCCA ATCATATGAA GAAATCTGCT 
TTTGGAAGAG GGAAAAGCCA AGGTTTCAAG AAAGGAAAAT CAAGAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ddx54 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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