diaph1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following diaph1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the diaph1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa54437 XM_031898780.1
Latest version!
Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa54438 XM_012959694.3
Latest version!
Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa54439 XM_031898781.1
Latest version!
Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa54440 XM_012959695.3
Latest version!
Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa28573 XM_012959693.2
Latest version!
Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OXa28574 XM_012959694.2
Latest version!
Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OXa28575 XM_012959695.2
Latest version!
Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa54437
    Clone ID Related Accession (Same CDS sequence) XM_031898780.1
    Accession Version XM_031898780.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3837bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein diaphanous homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
    AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA
    TTTACCTTTA AGCGGCTGAT GGTAGATGAG CTTGAACGAT TTACCAGTAT ACGGATGAAG
    AAAGAAAAGG AAAAGCCTTA TCCATCACAC AGAAACTCCA CCAACTTCCC AGATAACTCA
    AACCCCCTCC AAACCTTGCA GGAACTCGAA GATGATGTTG TGCTTCAGCT GTTTGAAAAA
    ATGCTGGATG ATATGAACCT CAATCCTGAG AAGCAGCAAC CGCTACGAGA AAAGGATATA
    ATGATAAAAA GAGAGATGGT TTCCCAATAT CTTCACACAT CCAAAGCTGG CATGAGCAAA
    AAGGAGAGTT CTCGTTCTGC ATTAATGTAC ATTCAAGACT TAAAATCCGG ACTAGGAGAC
    AATTCCCTCC TTTTGTGCTT AGAGTCACTA AGAGTATCTT TAAATAACAA TCCTGTCAGC
    TGGGTGCAGA ATTTTGGTGC AGAGGGTCTT TCGTGCCTTT TAGAAATTCT CAAAAGAATA
    CAGGATGAGC AAGCATCCTT GCGGGATGTT AGAAGCGAGC ATGAGATCAT CAGATGTCTG
    AAAGCTTTTA TGAATAACAA TTTTGGAATT AAGGCAATGC TGGGCACAGA AGATGGCATT
    TTGCTGTTAG CGAGAGCAGT AGATCGATCT GTCCCTGCAA TGATGATTGA TTCCTTGAAG
    CTGCTTTCTG CCCTGTGCAT CCTGCCACAA CCAGAGGACA TGCATGAGCG TGTTCTAGAA
    GCCCTTACGG AGAGAGCAGA GATGGAGGAA ATGGAACGAT TTAAACCTCT TCTGGATGGG
    TTAAACATTG CAAGTCCTGT GGCCTTGAAG GTGAGCTGTA TGCAGCTAAT CAATGCAGTG
    ATCAGCCAAG GAGAAGAGCT TGATTACAGA GTTCACATCC GAAGTGAATT GATGCGCTCG
    GGACTGGCCA AACTCCTAAA GGAACTACGT AATACAGAAA ATGAAGACCT GAAGGTACAA
    CTTGGTATTT TTGATGAGCA TTCTGAACTT GATTCTGATG ACTTGCGAGG ACGTTTGGAC
    GACATCCGCA TTGAAATGGA TGACTTCAGT GACGTCTTCC AAGTTCTTCT GAACACTGTC
    AAGGACTCCT CTGCAGAACC ATTATTTCTT TCCATCCTGC AGCATCTTCT TCTAATTAGA
    AATGATTATG ATGCCAGGCC GCAGTACTAT AAACTGATAG ATGAAGTTAT ATCACAGATA
    ATCCTTCAAA AAAATGGGGC TGATCCAGAT TTCAAATGCA AGCATGTAAA CATAGAAATC
    GAAAATCTGA TAGATCATTT TATCGACAAA AAGAAAGTAG AAACAAGCGA GTCAAAAGCA
    CGGGATCTGG TGAAAAAGTT GGACCTAGAG CTTACCGCAC GCCACGAACT CCAGGTGGAA
    ATGAAAGCCA AAGAGAGCGA CTTTGAGCTG AAAATAACAG AGCTGCAGGG GGAGAAGGAA
    TCTTTAGATG CAGAGAAGCA AGAAATCACT GCTGACAAGC AAAAAATGGA GACAGAACTC
    TCCCAACTTG TAGAGCAGGT TGAAAAATTG TCAAAGGAAC TTGAAGCTAC CAAAAAAGAA
    ATGGCATCAA TTTCCACATC TGCTGTTTTA ATCCCTGTAC CTTCTGCCTC TGGAAATGTG
    CCACCTCCAC CACCCCCTCC TCCTCCACTT CCAGGTGCAA CTGGTATTCC TCCACCTCCC
    CCACTTCCAG GTGGCTCTGG TATTCCTCCA CCACCCCCAC TTCCAGGTGG CTCTGGTATT
    GCTCCACCAC TTCCGGCTGG CTTTGGTATT CCTCCACCTC CCCCACTTCC GGGTGGCTTT
    GGTATTCCTC CACCTCCACC ACTTCCCGGT GGCTCTGGTA TTCCTCCACC TCCCCCACTT
    CCGGGTGGCT CTGGTATTCC TCCACCTCCC CCACTTCCGG GTGGCTCTGG TATTCCTCCA
    CCTCCCCCAC TTCCGGGTGG CTCTGGTATT CCTCCCCCAC CACCATTTCC GGGTGGCCCT
    GGAATTCCTC CCCCACCGCC ATTTCCAGGA GGCTTTGGAA TTCCTCCTCC TCCTCCTCCA
    TTTCCAGGTG GCCCTGGAAT TCCTCCTCCT CCTCCATTCC CTGGTGGCCC TGGGGTTCCA
    CCTCCACCTC CAGCATTTGG AGGTGGTGTT CCTCCACCAC CAGGACTTGG AGGCTGGGGG
    GCACCACCAC CCTTACCATT TGGCTTGGCT CCTAAGAAGA ATTATAAGCC AGAAGTGCAA
    CTGAAGCGAC CCAACTGGTC AAAGATCACT CCTGCTGAGC TTTCAGAGAA ATCGTTTTGG
    ACTCAGACAA AAGAAGATCG TTTTGAAAAA GAAGAGCTGT TTGCAAAAAT AACCCTCACT
    TTTTCTTCAC AGATCAAGTC TTCTAAAGCA AAAAAAGCAG AAGATGGTGA TGACAAAAAA
    GCAGCCCAGA AGAAAAAGAC AAAGGAGCTC AAAGTCTTGG ATTCCAAGAC AGCCCAAAAC
    CTATCAATCT TCCTTGGGTC GTTCCGCATG CCATACGAAG AGATCAAAAA TGTGATCCTA
    CAGGTCAATG AGGAAGTGCT GACTGAATCT ATGGTTTCAA ATCTGATCAA GCAGTTACCA
    GAACCAGATC AGTTACAGAT GTTAACAGAG TTCAAGAATG AGTACAAGGA TTTGGCAGAA
    CCAGAGCAGT TTGGAGTAGT GATGTGCAGT GTCCCCCGTT TGCGGCCACG ACTAAATGCC
    ATTCTCTTTA AACTTCTTTT TAATGAGCAA ATTGAAAATA TCAAGCCAGA CATTGTGTCA
    GTTACAGCTG CTTGCGAAGA AGTGCAAAAG TGCCAAAGCT TTGCTAGTCT CCTGGAAATC
    ACACTGCTGG TTGGGAACTT CATGAACGCA GGATCAAGGA ACGCTGATGC TTATGGCTTT
    GACATTAGCT TTCTCTGCAA GTTGAAAGAT ACCAAATCAG CGGACCAGAA GACCACACTG
    CTGCATTTCC TTGTTGAGAC TTGTGAGAAT GACTACCCTG ATGTACTGAA GTTTCCAGAT
    GAGATGGTTC ATGTAGAAAA GGCCAGTAGA GTGTCAGCTG AAACATTGCA AAAGAACCTG
    GATCAAATGA AAAAGCAGAT TGCAGACCTT CAAAGGGACA TTGATAGCTT CCCACCAACA
    GAAGATGAGA AGGATAAGTT TGTAGAAAAA ATGACCATAT TTGTCAAAGA AGCTCAAGAA
    CAGTATACTA AGCTTTGCAT GATGCACGAA AACATGCTGT CATTGTTCCA AGATTTGGGG
    AAATACTTTG TGTTTGACCC CAAGAAAACA TCCATTGAAG AGTTCTTCGC AGACCTGCAG
    AATTTCCGGA ACATGTTCTT GCACGCTCTG AAAGAGAACC AGAAGAGACG GGAAGCAGAA
    GAAAAGATGA AAAGAGCCAA ACTTGCCAAA GAGAAGGCAG AGAAGGAACG GTTAGAGAAG
    CAGCAGAAGA AAGAACAACT GATTGATATG AATGCAGAGG GTGACGAAAC TGGCGTAATG
    GACAGCCTGC TTGAAGCTTT GAATTCGGGA GCTGCATTCA GAAGGAAAAG GGGTCCTCGG
    CAAGCGAACA GAAAGGCCGG CTGTGCCGTT ACCTCACTGC TAGCATCTGA GCTCACTAAA
    GAAGATGCAA TGACCTCAGT GCCCACTAAG AAGAAAAAAC CCGCTGAGAC GGATTTGATG
    GAAACAGATG TAGGACTGCA CGAAGATGCC CAGGATATGC TTACGCGAGC AAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754640.1
    CDS208..4044
    Translation

    Target ORF information:

    RefSeq Version XM_031898780.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898780.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
    AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA
    TTTACCTTTA AGCGGCTGAT GGTAGATGAG CTTGAACGAT TTACCAGTAT ACGGATGAAG
    AAAGAAAAGG AAAAGCCTTA TCCATCACAC AGAAACTCCA CCAACTTCCC AGATAACTCA
    AACCCCCTCC AAACCTTGCA GGAACTCGAA GATGATGTTG TGCTTCAGCT GTTTGAAAAA
    ATGCTGGATG ATATGAACCT CAATCCTGAG AAGCAGCAAC CGCTACGAGA AAAGGATATA
    ATGATAAAAA GAGAGATGGT TTCCCAATAT CTTCACACAT CCAAAGCTGG CATGAGCAAA
    AAGGAGAGTT CTCGTTCTGC ATTAATGTAC ATTCAAGACT TAAAATCCGG ACTAGGAGAC
    AATTCCCTCC TTTTGTGCTT AGAGTCACTA AGAGTATCTT TAAATAACAA TCCTGTCAGC
    TGGGTGCAGA ATTTTGGTGC AGAGGGTCTT TCGTGCCTTT TAGAAATTCT CAAAAGAATA
    CAGGATGAGC AAGCATCCTT GCGGGATGTT AGAAGCGAGC ATGAGATCAT CAGATGTCTG
    AAAGCTTTTA TGAATAACAA TTTTGGAATT AAGGCAATGC TGGGCACAGA AGATGGCATT
    TTGCTGTTAG CGAGAGCAGT AGATCGATCT GTCCCTGCAA TGATGATTGA TTCCTTGAAG
    CTGCTTTCTG CCCTGTGCAT CCTGCCACAA CCAGAGGACA TGCATGAGCG TGTTCTAGAA
    GCCCTTACGG AGAGAGCAGA GATGGAGGAA ATGGAACGAT TTAAACCTCT TCTGGATGGG
    TTAAACATTG CAAGTCCTGT GGCCTTGAAG GTGAGCTGTA TGCAGCTAAT CAATGCAGTG
    ATCAGCCAAG GAGAAGAGCT TGATTACAGA GTTCACATCC GAAGTGAATT GATGCGCTCG
    GGACTGGCCA AACTCCTAAA GGAACTACGT AATACAGAAA ATGAAGACCT GAAGGTACAA
    CTTGGTATTT TTGATGAGCA TTCTGAACTT GATTCTGATG ACTTGCGAGG ACGTTTGGAC
    GACATCCGCA TTGAAATGGA TGACTTCAGT GACGTCTTCC AAGTTCTTCT GAACACTGTC
    AAGGACTCCT CTGCAGAACC ATTATTTCTT TCCATCCTGC AGCATCTTCT TCTAATTAGA
    AATGATTATG ATGCCAGGCC GCAGTACTAT AAACTGATAG ATGAAGTTAT ATCACAGATA
    ATCCTTCAAA AAAATGGGGC TGATCCAGAT TTCAAATGCA AGCATGTAAA CATAGAAATC
    GAAAATCTGA TAGATCATTT TATCGACAAA AAGAAAGTAG AAACAAGCGA GTCAAAAGCA
    CGGGATCTGG TGAAAAAGTT GGACCTAGAG CTTACCGCAC GCCACGAACT CCAGGTGGAA
    ATGAAAGCCA AAGAGAGCGA CTTTGAGCTG AAAATAACAG AGCTGCAGGG GGAGAAGGAA
    TCTTTAGATG CAGAGAAGCA AGAAATCACT GCTGACAAGC AAAAAATGGA GACAGAACTC
    TCCCAACTTG TAGAGCAGGT TGAAAAATTG TCAAAGGAAC TTGAAGCTAC CAAAAAAGAA
    ATGGCATCAA TTTCCACATC TGCTGTTTTA ATCCCTGTAC CTTCTGCCTC TGGAAATGTG
    CCACCTCCAC CACCCCCTCC TCCTCCACTT CCAGGTGCAA CTGGTATTCC TCCACCTCCC
    CCACTTCCAG GTGGCTCTGG TATTCCTCCA CCACCCCCAC TTCCAGGTGG CTCTGGTATT
    GCTCCACCAC TTCCGGCTGG CTTTGGTATT CCTCCACCTC CCCCACTTCC GGGTGGCTTT
    GGTATTCCTC CACCTCCACC ACTTCCCGGT GGCTCTGGTA TTCCTCCACC TCCCCCACTT
    CCGGGTGGCT CTGGTATTCC TCCACCTCCC CCACTTCCGG GTGGCTCTGG TATTCCTCCA
    CCTCCCCCAC TTCCGGGTGG CTCTGGTATT CCTCCCCCAC CACCATTTCC GGGTGGCCCT
    GGAATTCCTC CCCCACCGCC ATTTCCAGGA GGCTTTGGAA TTCCTCCTCC TCCTCCTCCA
    TTTCCAGGTG GCCCTGGAAT TCCTCCTCCT CCTCCATTCC CTGGTGGCCC TGGGGTTCCA
    CCTCCACCTC CAGCATTTGG AGGTGGTGTT CCTCCACCAC CAGGACTTGG AGGCTGGGGG
    GCACCACCAC CCTTACCATT TGGCTTGGCT CCTAAGAAGA ATTATAAGCC AGAAGTGCAA
    CTGAAGCGAC CCAACTGGTC AAAGATCACT CCTGCTGAGC TTTCAGAGAA ATCGTTTTGG
    ACTCAGACAA AAGAAGATCG TTTTGAAAAA GAAGAGCTGT TTGCAAAAAT AACCCTCACT
    TTTTCTTCAC AGATCAAGTC TTCTAAAGCA AAAAAAGCAG AAGATGGTGA TGACAAAAAA
    GCAGCCCAGA AGAAAAAGAC AAAGGAGCTC AAAGTCTTGG ATTCCAAGAC AGCCCAAAAC
    CTATCAATCT TCCTTGGGTC GTTCCGCATG CCATACGAAG AGATCAAAAA TGTGATCCTA
    CAGGTCAATG AGGAAGTGCT GACTGAATCT ATGGTTTCAA ATCTGATCAA GCAGTTACCA
    GAACCAGATC AGTTACAGAT GTTAACAGAG TTCAAGAATG AGTACAAGGA TTTGGCAGAA
    CCAGAGCAGT TTGGAGTAGT GATGTGCAGT GTCCCCCGTT TGCGGCCACG ACTAAATGCC
    ATTCTCTTTA AACTTCTTTT TAATGAGCAA ATTGAAAATA TCAAGCCAGA CATTGTGTCA
    GTTACAGCTG CTTGCGAAGA AGTGCAAAAG TGCCAAAGCT TTGCTAGTCT CCTGGAAATC
    ACACTGCTGG TTGGGAACTT CATGAACGCA GGATCAAGGA ACGCTGATGC TTATGGCTTT
    GACATTAGCT TTCTCTGCAA GTTGAAAGAT ACCAAATCAG CGGACCAGAA GACCACACTG
    CTGCATTTCC TTGTTGAGAC TTGTGAGAAT GACTACCCTG ATGTACTGAA GTTTCCAGAT
    GAGATGGTTC ATGTAGAAAA GGCCAGTAGA GTGTCAGCTG AAACATTGCA AAAGAACCTG
    GATCAAATGA AAAAGCAGAT TGCAGACCTT CAAAGGGACA TTGATAGCTT CCCACCAACA
    GAAGATGAGA AGGATAAGTT TGTAGAAAAA ATGACCATAT TTGTCAAAGA AGCTCAAGAA
    CAGTATACTA AGCTTTGCAT GATGCACGAA AACATGCTGT CATTGTTCCA AGATTTGGGG
    AAATACTTTG TGTTTGACCC CAAGAAAACA TCCATTGAAG AGTTCTTCGC AGACCTGCAG
    AATTTCCGGA ACATGTTCTT GCACGCTCTG AAAGAGAACC AGAAGAGACG GGAAGCAGAA
    GAAAAGATGA AAAGAGCCAA ACTTGCCAAA GAGAAGGCAG AGAAGGAACG GTTAGAGAAG
    CAGCAGAAGA AAGAACAACT GATTGATATG AATGCAGAGG GTGACGAAAC TGGCGTAATG
    GACAGCCTGC TTGAAGCTTT GAATTCGGGA GCTGCATTCA GAAGGAAAAG GGGTCCTCGG
    CAAGCGAACA GAAAGGCCGG CTGTGCCGTT ACCTCACTGC TAGCATCTGA GCTCACTAAA
    GAAGATGCAA TGACCTCAGT GCCCACTAAG AAGAAAAAAC CCGCTGAGAC GGATTTGATG
    GAAACAGATG TAGGACTGCA CGAAGATGCC CAGGATATGC TTACGCGAGC AAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54438
    Clone ID Related Accession (Same CDS sequence) XM_012959694.3
    Accession Version XM_012959694.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3810bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein diaphanous homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959694.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
    AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA
    TTTCTTGAAC GATTTACCAG TATACGGATG AAGAAAGAAA AGGAAAAGCC TTATCCATCA
    CACAGAAACT CCACCAACTT CCCAGATAAC TCAAACCCCC TCCAAACCTT GCAGGAACTC
    GAAGATGATG TTGTGCTTCA GCTGTTTGAA AAAATGCTGG ATGATATGAA CCTCAATCCT
    GAGAAGCAGC AACCGCTACG AGAAAAGGAT ATAATGATAA AAAGAGAGAT GGTTTCCCAA
    TATCTTCACA CATCCAAAGC TGGCATGAGC AAAAAGGAGA GTTCTCGTTC TGCATTAATG
    TACATTCAAG ACTTAAAATC CGGACTAGGA GACAATTCCC TCCTTTTGTG CTTAGAGTCA
    CTAAGAGTAT CTTTAAATAA CAATCCTGTC AGCTGGGTGC AGAATTTTGG TGCAGAGGGT
    CTTTCGTGCC TTTTAGAAAT TCTCAAAAGA ATACAGGATG AGCAAGCATC CTTGCGGGAT
    GTTAGAAGCG AGCATGAGAT CATCAGATGT CTGAAAGCTT TTATGAATAA CAATTTTGGA
    ATTAAGGCAA TGCTGGGCAC AGAAGATGGC ATTTTGCTGT TAGCGAGAGC AGTAGATCGA
    TCTGTCCCTG CAATGATGAT TGATTCCTTG AAGCTGCTTT CTGCCCTGTG CATCCTGCCA
    CAACCAGAGG ACATGCATGA GCGTGTTCTA GAAGCCCTTA CGGAGAGAGC AGAGATGGAG
    GAAATGGAAC GATTTAAACC TCTTCTGGAT GGGTTAAACA TTGCAAGTCC TGTGGCCTTG
    AAGGTGAGCT GTATGCAGCT AATCAATGCA GTGATCAGCC AAGGAGAAGA GCTTGATTAC
    AGAGTTCACA TCCGAAGTGA ATTGATGCGC TCGGGACTGG CCAAACTCCT AAAGGAACTA
    CGTAATACAG AAAATGAAGA CCTGAAGGTA CAACTTGGTA TTTTTGATGA GCATTCTGAA
    CTTGATTCTG ATGACTTGCG AGGACGTTTG GACGACATCC GCATTGAAAT GGATGACTTC
    AGTGACGTCT TCCAAGTTCT TCTGAACACT GTCAAGGACT CCTCTGCAGA ACCATTATTT
    CTTTCCATCC TGCAGCATCT TCTTCTAATT AGAAATGATT ATGATGCCAG GCCGCAGTAC
    TATAAACTGA TAGATGAAGT TATATCACAG ATAATCCTTC AAAAAAATGG GGCTGATCCA
    GATTTCAAAT GCAAGCATGT AAACATAGAA ATCGAAAATC TGATAGATCA TTTTATCGAC
    AAAAAGAAAG TAGAAACAAG CGAGTCAAAA GCACGGGATC TGGTGAAAAA GTTGGACCTA
    GAGCTTACCG CACGCCACGA ACTCCAGGTG GAAATGAAAG CCAAAGAGAG CGACTTTGAG
    CTGAAAATAA CAGAGCTGCA GGGGGAGAAG GAATCTTTAG ATGCAGAGAA GCAAGAAATC
    ACTGCTGACA AGCAAAAAAT GGAGACAGAA CTCTCCCAAC TTGTAGAGCA GGTTGAAAAA
    TTGTCAAAGG AACTTGAAGC TACCAAAAAA GAAATGGCAT CAATTTCCAC ATCTGCTGTT
    TTAATCCCTG TACCTTCTGC CTCTGGAAAT GTGCCACCTC CACCACCCCC TCCTCCTCCA
    CTTCCAGGTG CAACTGGTAT TCCTCCACCT CCCCCACTTC CAGGTGGCTC TGGTATTCCT
    CCACCACCCC CACTTCCAGG TGGCTCTGGT ATTGCTCCAC CACTTCCGGC TGGCTTTGGT
    ATTCCTCCAC CTCCCCCACT TCCGGGTGGC TTTGGTATTC CTCCACCTCC ACCACTTCCC
    GGTGGCTCTG GTATTCCTCC ACCTCCCCCA CTTCCGGGTG GCTCTGGTAT TCCTCCACCT
    CCCCCACTTC CGGGTGGCTC TGGTATTCCT CCACCTCCCC CACTTCCGGG TGGCTCTGGT
    ATTCCTCCCC CACCACCATT TCCGGGTGGC CCTGGAATTC CTCCCCCACC GCCATTTCCA
    GGAGGCTTTG GAATTCCTCC TCCTCCTCCT CCATTTCCAG GTGGCCCTGG AATTCCTCCT
    CCTCCTCCAT TCCCTGGTGG CCCTGGGGTT CCACCTCCAC CTCCAGCATT TGGAGGTGGT
    GTTCCTCCAC CACCAGGACT TGGAGGCTGG GGGGCACCAC CACCCTTACC ATTTGGCTTG
    GCTCCTAAGA AGAATTATAA GCCAGAAGTG CAACTGAAGC GACCCAACTG GTCAAAGATC
    ACTCCTGCTG AGCTTTCAGA GAAATCGTTT TGGACTCAGA CAAAAGAAGA TCGTTTTGAA
    AAAGAAGAGC TGTTTGCAAA AATAACCCTC ACTTTTTCTT CACAGATCAA GTCTTCTAAA
    GCAAAAAAAG CAGAAGATGG TGATGACAAA AAAGCAGCCC AGAAGAAAAA GACAAAGGAG
    CTCAAAGTCT TGGATTCCAA GACAGCCCAA AACCTATCAA TCTTCCTTGG GTCGTTCCGC
    ATGCCATACG AAGAGATCAA AAATGTGATC CTACAGGTCA ATGAGGAAGT GCTGACTGAA
    TCTATGGTTT CAAATCTGAT CAAGCAGTTA CCAGAACCAG ATCAGTTACA GATGTTAACA
    GAGTTCAAGA ATGAGTACAA GGATTTGGCA GAACCAGAGC AGTTTGGAGT AGTGATGTGC
    AGTGTCCCCC GTTTGCGGCC ACGACTAAAT GCCATTCTCT TTAAACTTCT TTTTAATGAG
    CAAATTGAAA ATATCAAGCC AGACATTGTG TCAGTTACAG CTGCTTGCGA AGAAGTGCAA
    AAGTGCCAAA GCTTTGCTAG TCTCCTGGAA ATCACACTGC TGGTTGGGAA CTTCATGAAC
    GCAGGATCAA GGAACGCTGA TGCTTATGGC TTTGACATTA GCTTTCTCTG CAAGTTGAAA
    GATACCAAAT CAGCGGACCA GAAGACCACA CTGCTGCATT TCCTTGTTGA GACTTGTGAG
    AATGACTACC CTGATGTACT GAAGTTTCCA GATGAGATGG TTCATGTAGA AAAGGCCAGT
    AGAGTGTCAG CTGAAACATT GCAAAAGAAC CTGGATCAAA TGAAAAAGCA GATTGCAGAC
    CTTCAAAGGG ACATTGATAG CTTCCCACCA ACAGAAGATG AGAAGGATAA GTTTGTAGAA
    AAAATGACAA TATTTGTCAA AGAAGCTCAA GAACAGTATA CTAAGCTTTG CATGATGCAC
    GAAAACATGC TGTCATTGTT CCAAGATTTG GGGAAATACT TTGTGTTTGA CCCCAAGAAA
    ACATCCATTG AAGAGTTCTT CGCAGACCTG CAGAATTTCC GGAACATGTT CTTGCACGCT
    CTGAAAGAGA ACCAGAAGAG ACGGGAAGCA GAAGAAAAGA TGAAAAGAGC CAAACTTGCC
    AAAGAGAAGG CAGAGAAGGA ACGGTTAGAG AAGCAGCAGA AGAAAGAACA ACTGATTGAT
    ATGAATGCAG AGGGTGACGA AACTGGCGTA ATGGACAGCC TGCTTGAAGC TTTGAATTCG
    GGAGCTGCAT TCAGAAGGAA AAGGGGTCCT CGGCAAGCGA ACAGAAAGGC CGGCTGTGCC
    GTTACCTCAC TGCTAGCATC TGAGCTCACT AAAGAAGATG CAATGACCTC AGTGCCCACT
    AAGAAGAAAA AACCCGCTGA GACGGATTTG ATGGAAACAG ATGTAGGACT GCACGAAGAT
    GCCCAGGATA TGCTTACGCG AGCAAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815148.2
    CDS316..4125
    Translation

    Target ORF information:

    RefSeq Version XM_012959694.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959694.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
    AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA
    TTTCTTGAAC GATTTACCAG TATACGGATG AAGAAAGAAA AGGAAAAGCC TTATCCATCA
    CACAGAAACT CCACCAACTT CCCAGATAAC TCAAACCCCC TCCAAACCTT GCAGGAACTC
    GAAGATGATG TTGTGCTTCA GCTGTTTGAA AAAATGCTGG ATGATATGAA CCTCAATCCT
    GAGAAGCAGC AACCGCTACG AGAAAAGGAT ATAATGATAA AAAGAGAGAT GGTTTCCCAA
    TATCTTCACA CATCCAAAGC TGGCATGAGC AAAAAGGAGA GTTCTCGTTC TGCATTAATG
    TACATTCAAG ACTTAAAATC CGGACTAGGA GACAATTCCC TCCTTTTGTG CTTAGAGTCA
    CTAAGAGTAT CTTTAAATAA CAATCCTGTC AGCTGGGTGC AGAATTTTGG TGCAGAGGGT
    CTTTCGTGCC TTTTAGAAAT TCTCAAAAGA ATACAGGATG AGCAAGCATC CTTGCGGGAT
    GTTAGAAGCG AGCATGAGAT CATCAGATGT CTGAAAGCTT TTATGAATAA CAATTTTGGA
    ATTAAGGCAA TGCTGGGCAC AGAAGATGGC ATTTTGCTGT TAGCGAGAGC AGTAGATCGA
    TCTGTCCCTG CAATGATGAT TGATTCCTTG AAGCTGCTTT CTGCCCTGTG CATCCTGCCA
    CAACCAGAGG ACATGCATGA GCGTGTTCTA GAAGCCCTTA CGGAGAGAGC AGAGATGGAG
    GAAATGGAAC GATTTAAACC TCTTCTGGAT GGGTTAAACA TTGCAAGTCC TGTGGCCTTG
    AAGGTGAGCT GTATGCAGCT AATCAATGCA GTGATCAGCC AAGGAGAAGA GCTTGATTAC
    AGAGTTCACA TCCGAAGTGA ATTGATGCGC TCGGGACTGG CCAAACTCCT AAAGGAACTA
    CGTAATACAG AAAATGAAGA CCTGAAGGTA CAACTTGGTA TTTTTGATGA GCATTCTGAA
    CTTGATTCTG ATGACTTGCG AGGACGTTTG GACGACATCC GCATTGAAAT GGATGACTTC
    AGTGACGTCT TCCAAGTTCT TCTGAACACT GTCAAGGACT CCTCTGCAGA ACCATTATTT
    CTTTCCATCC TGCAGCATCT TCTTCTAATT AGAAATGATT ATGATGCCAG GCCGCAGTAC
    TATAAACTGA TAGATGAAGT TATATCACAG ATAATCCTTC AAAAAAATGG GGCTGATCCA
    GATTTCAAAT GCAAGCATGT AAACATAGAA ATCGAAAATC TGATAGATCA TTTTATCGAC
    AAAAAGAAAG TAGAAACAAG CGAGTCAAAA GCACGGGATC TGGTGAAAAA GTTGGACCTA
    GAGCTTACCG CACGCCACGA ACTCCAGGTG GAAATGAAAG CCAAAGAGAG CGACTTTGAG
    CTGAAAATAA CAGAGCTGCA GGGGGAGAAG GAATCTTTAG ATGCAGAGAA GCAAGAAATC
    ACTGCTGACA AGCAAAAAAT GGAGACAGAA CTCTCCCAAC TTGTAGAGCA GGTTGAAAAA
    TTGTCAAAGG AACTTGAAGC TACCAAAAAA GAAATGGCAT CAATTTCCAC ATCTGCTGTT
    TTAATCCCTG TACCTTCTGC CTCTGGAAAT GTGCCACCTC CACCACCCCC TCCTCCTCCA
    CTTCCAGGTG CAACTGGTAT TCCTCCACCT CCCCCACTTC CAGGTGGCTC TGGTATTCCT
    CCACCACCCC CACTTCCAGG TGGCTCTGGT ATTGCTCCAC CACTTCCGGC TGGCTTTGGT
    ATTCCTCCAC CTCCCCCACT TCCGGGTGGC TTTGGTATTC CTCCACCTCC ACCACTTCCC
    GGTGGCTCTG GTATTCCTCC ACCTCCCCCA CTTCCGGGTG GCTCTGGTAT TCCTCCACCT
    CCCCCACTTC CGGGTGGCTC TGGTATTCCT CCACCTCCCC CACTTCCGGG TGGCTCTGGT
    ATTCCTCCCC CACCACCATT TCCGGGTGGC CCTGGAATTC CTCCCCCACC GCCATTTCCA
    GGAGGCTTTG GAATTCCTCC TCCTCCTCCT CCATTTCCAG GTGGCCCTGG AATTCCTCCT
    CCTCCTCCAT TCCCTGGTGG CCCTGGGGTT CCACCTCCAC CTCCAGCATT TGGAGGTGGT
    GTTCCTCCAC CACCAGGACT TGGAGGCTGG GGGGCACCAC CACCCTTACC ATTTGGCTTG
    GCTCCTAAGA AGAATTATAA GCCAGAAGTG CAACTGAAGC GACCCAACTG GTCAAAGATC
    ACTCCTGCTG AGCTTTCAGA GAAATCGTTT TGGACTCAGA CAAAAGAAGA TCGTTTTGAA
    AAAGAAGAGC TGTTTGCAAA AATAACCCTC ACTTTTTCTT CACAGATCAA GTCTTCTAAA
    GCAAAAAAAG CAGAAGATGG TGATGACAAA AAAGCAGCCC AGAAGAAAAA GACAAAGGAG
    CTCAAAGTCT TGGATTCCAA GACAGCCCAA AACCTATCAA TCTTCCTTGG GTCGTTCCGC
    ATGCCATACG AAGAGATCAA AAATGTGATC CTACAGGTCA ATGAGGAAGT GCTGACTGAA
    TCTATGGTTT CAAATCTGAT CAAGCAGTTA CCAGAACCAG ATCAGTTACA GATGTTAACA
    GAGTTCAAGA ATGAGTACAA GGATTTGGCA GAACCAGAGC AGTTTGGAGT AGTGATGTGC
    AGTGTCCCCC GTTTGCGGCC ACGACTAAAT GCCATTCTCT TTAAACTTCT TTTTAATGAG
    CAAATTGAAA ATATCAAGCC AGACATTGTG TCAGTTACAG CTGCTTGCGA AGAAGTGCAA
    AAGTGCCAAA GCTTTGCTAG TCTCCTGGAA ATCACACTGC TGGTTGGGAA CTTCATGAAC
    GCAGGATCAA GGAACGCTGA TGCTTATGGC TTTGACATTA GCTTTCTCTG CAAGTTGAAA
    GATACCAAAT CAGCGGACCA GAAGACCACA CTGCTGCATT TCCTTGTTGA GACTTGTGAG
    AATGACTACC CTGATGTACT GAAGTTTCCA GATGAGATGG TTCATGTAGA AAAGGCCAGT
    AGAGTGTCAG CTGAAACATT GCAAAAGAAC CTGGATCAAA TGAAAAAGCA GATTGCAGAC
    CTTCAAAGGG ACATTGATAG CTTCCCACCA ACAGAAGATG AGAAGGATAA GTTTGTAGAA
    AAAATGACAA TATTTGTCAA AGAAGCTCAA GAACAGTATA CTAAGCTTTG CATGATGCAC
    GAAAACATGC TGTCATTGTT CCAAGATTTG GGGAAATACT TTGTGTTTGA CCCCAAGAAA
    ACATCCATTG AAGAGTTCTT CGCAGACCTG CAGAATTTCC GGAACATGTT CTTGCACGCT
    CTGAAAGAGA ACCAGAAGAG ACGGGAAGCA GAAGAAAAGA TGAAAAGAGC CAAACTTGCC
    AAAGAGAAGG CAGAGAAGGA ACGGTTAGAG AAGCAGCAGA AGAAAGAACA ACTGATTGAT
    ATGAATGCAG AGGGTGACGA AACTGGCGTA ATGGACAGCC TGCTTGAAGC TTTGAATTCG
    GGAGCTGCAT TCAGAAGGAA AAGGGGTCCT CGGCAAGCGA ACAGAAAGGC CGGCTGTGCC
    GTTACCTCAC TGCTAGCATC TGAGCTCACT AAAGAAGATG CAATGACCTC AGTGCCCACT
    AAGAAGAAAA AACCCGCTGA GACGGATTTG ATGGAAACAG ATGTAGGACT GCACGAAGAT
    GCCCAGGATA TGCTTACGCG AGCAAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54439
    Clone ID Related Accession (Same CDS sequence) XM_031898781.1
    Accession Version XM_031898781.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3807bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein diaphanous homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
    AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA
    TTTACCTTTA AGCGGCTGAT GGTAGATGAG CTTGAACGAT TTACCAGTAT ACGGATGAAG
    AAAGAAAAGG AAAAGCCTTA TCCATCACAC AGAAACTCCA CCAACTTCCC AGATAACTCA
    AACCCCCTCC AAACCTTGCA GGAACTCGAA GATGATGTTG TGCTTCAGCT GTTTGAAAAA
    ATGCTGGATG ATATGAACCT CAATCCTGAG AAGCAGCAAC CGCTACGAGA AAAGGATATA
    ATGATAAAAA GAGAGATGGT TTCCCAATAT CTTCACACAT CCAAAGCTGG CATGAGCAAA
    AAGGAGAGTT CTCGTTCTGC ATTAATGTAC ATTCAAGACT TAAAATCCGG ACTAGGAGAC
    AATTCCCTCC TTTTGTGCTT AGAGTCACTA AGAGTATCTT TAAATAACAA TCCTGTCAGC
    TGGGTGCAGA ATTTTGGTGC AGAGGGTCTT TCGTGCCTTT TAGAAATTCT CAAAAGAATA
    CAGGATGAGC AAGCATCCTT GCGGGATGTT AGAAGCGAGC ATGAGATCAT CAGATGTCTG
    AAAGCTTTTA TGAATAACAA TTTTGGAATT AAGGCAATGC TGGGCACAGA AGATGGCATT
    TTGCTGTTAG CGAGAGCAGT AGATCGATCT GTCCCTGCAA TGATGATTGA TTCCTTGAAG
    CTGCTTTCTG CCCTGTGCAT CCTGCCACAA CCAGAGGACA TGCATGAGCG TGTTCTAGAA
    GCCCTTACGG AGAGAGCAGA GATGGAGGAA ATGGAACGAT TTAAACCTCT TCTGGATGGG
    TTAAACATTG CAAGTCCTGT GGCCTTGAAG GTGAGCTGTA TGCAGCTAAT CAATGCAGTG
    ATCAGCCAAG GAGAAGAGCT TGATTACAGA GTTCACATCC GAAGTGAATT GATGCGCTCG
    GGACTGGCCA AACTCCTAAA GGAACTACGT AATACAGAAA ATGAAGACCT GAAGGTACAA
    CTTGGTATTT TTGATGAGCA TTCTGAACTT GATTCTGATG ACTTGCGAGG ACGTTTGGAC
    GACATCCGCA TTGAAATGGA TGACTTCAGT GACGTCTTCC AAGTTCTTCT GAACACTGTC
    AAGGACTCCT CTGCAGAACC ATTATTTCTT TCCATCCTGC AGCATCTTCT TCTAATTAGA
    AATGATTATG ATGCCAGGCC GCAGTACTAT AAACTGATAG ATGAAGTTAT ATCACAGATA
    ATCCTTCAAA AAAATGGGGC TGATCCAGAT TTCAAATGCA AGCATGTAAA CATAGAAATC
    GAAAATCTGA TAGATCATTT TATCGACAAA AAGAAAGTAG AAACAAGCGA GTCAAAAGCA
    CGGGATCTGG TGAAAAAGTT GGACCTAGAG CTTACCGCAC GCCACGAACT CCAGGTGGAA
    ATGAAAGCCA AAGAGAGCGA CTTTGAGCTG AAAATAACAG AGCTGCAGGG GGAGAAGGAA
    TCTTTAGATG CAGAGAAGCA AGAAATCACT GCTGACAAGC AAAAAATGGA GACAGAACTC
    TCCCAACTTG TAGAGCAGGT TGAAAAATTG TCAAAGGAAC TTGAAGCTAC CAAAAAAGAA
    ATGGCATCAA TTTCCACATC TGCTGTTTTA ATCCCTGTAC CTTCTGCCTC TGGAAATGTG
    CCACCTCCAC CACCCCCTCC TCCTCCACTT CCAGGTGCAA CTGGTATTCC TCCACCTCCC
    CCACTTCCAG GTGGCTCTGG TATTCCTCCA CCACCCCCAC TTCCAGGTGG CTCTGGTATT
    CCTCCACCTC CCCCACTTCC GGGTGGCTTT GGTATTCCTC CACCTCCACC ACTTCCCGGT
    GGCTCTGGTA TTCCTCCACC TCCCCCACTT CCGGGTGGCT CTGGTATTCC TCCACCTCCC
    CCACTTCCGG GTGGCTCTGG TATTCCTCCA CCTCCCCCAC TTCCGGGTGG CTCTGGTATT
    CCTCCCCCAC CACCATTTCC GGGTGGCCCT GGAATTCCTC CCCCACCGCC ATTTCCAGGA
    GGCTTTGGAA TTCCTCCTCC TCCTCCTCCA TTTCCAGGTG GCCCTGGAAT TCCTCCTCCT
    CCTCCATTCC CTGGTGGCCC TGGGGTTCCA CCTCCACCTC CAGCATTTGG AGGTGGTGTT
    CCTCCACCAC CAGGACTTGG AGGCTGGGGG GCACCACCAC CCTTACCATT TGGCTTGGCT
    CCTAAGAAGA ATTATAAGCC AGAAGTGCAA CTGAAGCGAC CCAACTGGTC AAAGATCACT
    CCTGCTGAGC TTTCAGAGAA ATCGTTTTGG ACTCAGACAA AAGAAGATCG TTTTGAAAAA
    GAAGAGCTGT TTGCAAAAAT AACCCTCACT TTTTCTTCAC AGATCAAGTC TTCTAAAGCA
    AAAAAAGCAG AAGATGGTGA TGACAAAAAA GCAGCCCAGA AGAAAAAGAC AAAGGAGCTC
    AAAGTCTTGG ATTCCAAGAC AGCCCAAAAC CTATCAATCT TCCTTGGGTC GTTCCGCATG
    CCATACGAAG AGATCAAAAA TGTGATCCTA CAGGTCAATG AGGAAGTGCT GACTGAATCT
    ATGGTTTCAA ATCTGATCAA GCAGTTACCA GAACCAGATC AGTTACAGAT GTTAACAGAG
    TTCAAGAATG AGTACAAGGA TTTGGCAGAA CCAGAGCAGT TTGGAGTAGT GATGTGCAGT
    GTCCCCCGTT TGCGGCCACG ACTAAATGCC ATTCTCTTTA AACTTCTTTT TAATGAGCAA
    ATTGAAAATA TCAAGCCAGA CATTGTGTCA GTTACAGCTG CTTGCGAAGA AGTGCAAAAG
    TGCCAAAGCT TTGCTAGTCT CCTGGAAATC ACACTGCTGG TTGGGAACTT CATGAACGCA
    GGATCAAGGA ACGCTGATGC TTATGGCTTT GACATTAGCT TTCTCTGCAA GTTGAAAGAT
    ACCAAATCAG CGGACCAGAA GACCACACTG CTGCATTTCC TTGTTGAGAC TTGTGAGAAT
    GACTACCCTG ATGTACTGAA GTTTCCAGAT GAGATGGTTC ATGTAGAAAA GGCCAGTAGA
    GTGTCAGCTG AAACATTGCA AAAGAACCTG GATCAAATGA AAAAGCAGAT TGCAGACCTT
    CAAAGGGACA TTGATAGCTT CCCACCAACA GAAGATGAGA AGGATAAGTT TGTAGAAAAA
    ATGACAATAT TTGTCAAAGA AGCTCAAGAA CAGTATACTA AGCTTTGCAT GATGCACGAA
    AACATGCTGT CATTGTTCCA AGATTTGGGG AAATACTTTG TGTTTGACCC CAAGAAAACA
    TCCATTGAAG AGTTCTTCGC AGACCTGCAG AATTTCCGGA ACATGTTCTT GCACGCTCTG
    AAAGAGAACC AGAAGAGACG GGAAGCAGAA GAAAAGATGA AAAGAGCCAA ACTTGCCAAA
    GAGAAGGCAG AGAAGGAACG GTTAGAGAAG CAGCAGAAGA AAGAACAACT GATTGATATG
    AATGCAGAGG GTGACGAAAC TGGCGTAATG GACAGCCTGC TTGAAGCTTT GAATTCGGGA
    GCTGCATTCA GAAGGAAAAG GGGTCCTCGG CAAGCGAACA GAAAGGCCGG CTGTGCCGTT
    ACCTCACTGC TAGCATCTGA GCTCACTAAA GAAGATGCAA TGACCTCAGT GCCCACTAAG
    AAGAAAAAAC CCGCTGAGAC GGATTTGATG GAAACAGATG TAGGACTGCA CGAAGATGCC
    CAGGATATGC TTACGCGAGC AAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754641.1
    CDS208..4014
    Translation

    Target ORF information:

    RefSeq Version XM_031898781.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898781.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
    AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA
    TTTACCTTTA AGCGGCTGAT GGTAGATGAG CTTGAACGAT TTACCAGTAT ACGGATGAAG
    AAAGAAAAGG AAAAGCCTTA TCCATCACAC AGAAACTCCA CCAACTTCCC AGATAACTCA
    AACCCCCTCC AAACCTTGCA GGAACTCGAA GATGATGTTG TGCTTCAGCT GTTTGAAAAA
    ATGCTGGATG ATATGAACCT CAATCCTGAG AAGCAGCAAC CGCTACGAGA AAAGGATATA
    ATGATAAAAA GAGAGATGGT TTCCCAATAT CTTCACACAT CCAAAGCTGG CATGAGCAAA
    AAGGAGAGTT CTCGTTCTGC ATTAATGTAC ATTCAAGACT TAAAATCCGG ACTAGGAGAC
    AATTCCCTCC TTTTGTGCTT AGAGTCACTA AGAGTATCTT TAAATAACAA TCCTGTCAGC
    TGGGTGCAGA ATTTTGGTGC AGAGGGTCTT TCGTGCCTTT TAGAAATTCT CAAAAGAATA
    CAGGATGAGC AAGCATCCTT GCGGGATGTT AGAAGCGAGC ATGAGATCAT CAGATGTCTG
    AAAGCTTTTA TGAATAACAA TTTTGGAATT AAGGCAATGC TGGGCACAGA AGATGGCATT
    TTGCTGTTAG CGAGAGCAGT AGATCGATCT GTCCCTGCAA TGATGATTGA TTCCTTGAAG
    CTGCTTTCTG CCCTGTGCAT CCTGCCACAA CCAGAGGACA TGCATGAGCG TGTTCTAGAA
    GCCCTTACGG AGAGAGCAGA GATGGAGGAA ATGGAACGAT TTAAACCTCT TCTGGATGGG
    TTAAACATTG CAAGTCCTGT GGCCTTGAAG GTGAGCTGTA TGCAGCTAAT CAATGCAGTG
    ATCAGCCAAG GAGAAGAGCT TGATTACAGA GTTCACATCC GAAGTGAATT GATGCGCTCG
    GGACTGGCCA AACTCCTAAA GGAACTACGT AATACAGAAA ATGAAGACCT GAAGGTACAA
    CTTGGTATTT TTGATGAGCA TTCTGAACTT GATTCTGATG ACTTGCGAGG ACGTTTGGAC
    GACATCCGCA TTGAAATGGA TGACTTCAGT GACGTCTTCC AAGTTCTTCT GAACACTGTC
    AAGGACTCCT CTGCAGAACC ATTATTTCTT TCCATCCTGC AGCATCTTCT TCTAATTAGA
    AATGATTATG ATGCCAGGCC GCAGTACTAT AAACTGATAG ATGAAGTTAT ATCACAGATA
    ATCCTTCAAA AAAATGGGGC TGATCCAGAT TTCAAATGCA AGCATGTAAA CATAGAAATC
    GAAAATCTGA TAGATCATTT TATCGACAAA AAGAAAGTAG AAACAAGCGA GTCAAAAGCA
    CGGGATCTGG TGAAAAAGTT GGACCTAGAG CTTACCGCAC GCCACGAACT CCAGGTGGAA
    ATGAAAGCCA AAGAGAGCGA CTTTGAGCTG AAAATAACAG AGCTGCAGGG GGAGAAGGAA
    TCTTTAGATG CAGAGAAGCA AGAAATCACT GCTGACAAGC AAAAAATGGA GACAGAACTC
    TCCCAACTTG TAGAGCAGGT TGAAAAATTG TCAAAGGAAC TTGAAGCTAC CAAAAAAGAA
    ATGGCATCAA TTTCCACATC TGCTGTTTTA ATCCCTGTAC CTTCTGCCTC TGGAAATGTG
    CCACCTCCAC CACCCCCTCC TCCTCCACTT CCAGGTGCAA CTGGTATTCC TCCACCTCCC
    CCACTTCCAG GTGGCTCTGG TATTCCTCCA CCACCCCCAC TTCCAGGTGG CTCTGGTATT
    CCTCCACCTC CCCCACTTCC GGGTGGCTTT GGTATTCCTC CACCTCCACC ACTTCCCGGT
    GGCTCTGGTA TTCCTCCACC TCCCCCACTT CCGGGTGGCT CTGGTATTCC TCCACCTCCC
    CCACTTCCGG GTGGCTCTGG TATTCCTCCA CCTCCCCCAC TTCCGGGTGG CTCTGGTATT
    CCTCCCCCAC CACCATTTCC GGGTGGCCCT GGAATTCCTC CCCCACCGCC ATTTCCAGGA
    GGCTTTGGAA TTCCTCCTCC TCCTCCTCCA TTTCCAGGTG GCCCTGGAAT TCCTCCTCCT
    CCTCCATTCC CTGGTGGCCC TGGGGTTCCA CCTCCACCTC CAGCATTTGG AGGTGGTGTT
    CCTCCACCAC CAGGACTTGG AGGCTGGGGG GCACCACCAC CCTTACCATT TGGCTTGGCT
    CCTAAGAAGA ATTATAAGCC AGAAGTGCAA CTGAAGCGAC CCAACTGGTC AAAGATCACT
    CCTGCTGAGC TTTCAGAGAA ATCGTTTTGG ACTCAGACAA AAGAAGATCG TTTTGAAAAA
    GAAGAGCTGT TTGCAAAAAT AACCCTCACT TTTTCTTCAC AGATCAAGTC TTCTAAAGCA
    AAAAAAGCAG AAGATGGTGA TGACAAAAAA GCAGCCCAGA AGAAAAAGAC AAAGGAGCTC
    AAAGTCTTGG ATTCCAAGAC AGCCCAAAAC CTATCAATCT TCCTTGGGTC GTTCCGCATG
    CCATACGAAG AGATCAAAAA TGTGATCCTA CAGGTCAATG AGGAAGTGCT GACTGAATCT
    ATGGTTTCAA ATCTGATCAA GCAGTTACCA GAACCAGATC AGTTACAGAT GTTAACAGAG
    TTCAAGAATG AGTACAAGGA TTTGGCAGAA CCAGAGCAGT TTGGAGTAGT GATGTGCAGT
    GTCCCCCGTT TGCGGCCACG ACTAAATGCC ATTCTCTTTA AACTTCTTTT TAATGAGCAA
    ATTGAAAATA TCAAGCCAGA CATTGTGTCA GTTACAGCTG CTTGCGAAGA AGTGCAAAAG
    TGCCAAAGCT TTGCTAGTCT CCTGGAAATC ACACTGCTGG TTGGGAACTT CATGAACGCA
    GGATCAAGGA ACGCTGATGC TTATGGCTTT GACATTAGCT TTCTCTGCAA GTTGAAAGAT
    ACCAAATCAG CGGACCAGAA GACCACACTG CTGCATTTCC TTGTTGAGAC TTGTGAGAAT
    GACTACCCTG ATGTACTGAA GTTTCCAGAT GAGATGGTTC ATGTAGAAAA GGCCAGTAGA
    GTGTCAGCTG AAACATTGCA AAAGAACCTG GATCAAATGA AAAAGCAGAT TGCAGACCTT
    CAAAGGGACA TTGATAGCTT CCCACCAACA GAAGATGAGA AGGATAAGTT TGTAGAAAAA
    ATGACAATAT TTGTCAAAGA AGCTCAAGAA CAGTATACTA AGCTTTGCAT GATGCACGAA
    AACATGCTGT CATTGTTCCA AGATTTGGGG AAATACTTTG TGTTTGACCC CAAGAAAACA
    TCCATTGAAG AGTTCTTCGC AGACCTGCAG AATTTCCGGA ACATGTTCTT GCACGCTCTG
    AAAGAGAACC AGAAGAGACG GGAAGCAGAA GAAAAGATGA AAAGAGCCAA ACTTGCCAAA
    GAGAAGGCAG AGAAGGAACG GTTAGAGAAG CAGCAGAAGA AAGAACAACT GATTGATATG
    AATGCAGAGG GTGACGAAAC TGGCGTAATG GACAGCCTGC TTGAAGCTTT GAATTCGGGA
    GCTGCATTCA GAAGGAAAAG GGGTCCTCGG CAAGCGAACA GAAAGGCCGG CTGTGCCGTT
    ACCTCACTGC TAGCATCTGA GCTCACTAAA GAAGATGCAA TGACCTCAGT GCCCACTAAG
    AAGAAAAAAC CCGCTGAGAC GGATTTGATG GAAACAGATG TAGGACTGCA CGAAGATGCC
    CAGGATATGC TTACGCGAGC AAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54440
    Clone ID Related Accession (Same CDS sequence) XM_012959695.3
    Accession Version XM_012959695.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein diaphanous homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959695.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
    AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA
    TTTACCTTTA AGCGGCTGAT GGTAGATGAG CTTGAACGAT TTACCAGTAT ACGGATGAAG
    AAAGAAAAGG AAAAGCCTTA TCCATCACAC AGAAACTCCA CCAACTTCCC AGATAACTCA
    AACCCCCTCC AAACCTTGCA GGAACTCGAA GATGATGTTG TGCTTCAGCT GTTTGAAAAA
    ATGCTGGATG ATATGAACCT CAATCCTGAG AAGCAGCAAC CGCTACGAGA AAAGGATATA
    ATGATAAAAA GAGAGATGGT TTCCCAATAT CTTCACACAT CCAAAGCTGG CATGAGCAAA
    AAGGAGAGTT CTCGTTCTGC ATTAATGTAC ATTCAAGACT TAAAATCCGG ACTAGGAGAC
    AATTCCCTCC TTTTGTGCTT AGAGTCACTA AGAGTATCTT TAAATAACAA TCCTGTCAGC
    TGGGTGCAGA ATTTTGGTGC AGAGGGTCTT TCGTGCCTTT TAGAAATTCT CAAAAGAATA
    CAGGATGAGC AAGCATCCTT GCGGGATGTT AGAAGCGAGC ATGAGATCAT CAGATGTCTG
    AAAGCTTTTA TGAATAACAA TTTTGGAATT AAGGCAATGC TGGGCACAGA AGATGGCATT
    TTGCTGTTAG CGAGAGCAGT AGATCGATCT GTCCCTGCAA TGATGATTGA TTCCTTGAAG
    CTGCTTTCTG CCCTGTGCAT CCTGCCACAA CCAGAGGACA TGCATGAGCG TGTTCTAGAA
    GCCCTTACGG AGAGAGCAGA GATGGAGGAA ATGGAACGAT TTAAACCTCT TCTGGATGGG
    TTAAACATTG CAAGTCCTGT GGCCTTGAAG GTGAGCTGTA TGCAGCTAAT CAATGCAGTG
    ATCAGCCAAG GAGAAGAGCT TGATTACAGA GTTCACATCC GAAGTGAATT GATGCGCTCG
    GGACTGGCCA AACTCCTAAA GGAACTACGT AATACAGAAA ATGAAGACCT GAAGGTACAA
    CTTGGTATTT TTGATGAGCA TTCTGAACTT GATTCTGATG ACTTGCGAGG ACGTTTGGAC
    GACATCCGCA TTGAAATGGA TGACTTCAGT GACGTCTTCC AAGTTCTTCT GAACACTGTC
    AAGGACTCCT CTGCAGAACC ATTATTTCTT TCCATCCTGC AGCATCTTCT TCTAATTAGA
    AATGATTATG ATGCCAGGCC GCAGTACTAT AAACTGATAG ATGAAGTTAT ATCACAGATA
    ATCCTTCAAA AAAATGGGGC TGATCCAGAT TTCAAATGCA AGCATGTAAA CATAGAAATC
    GAAAATCTGA TAGATCATTT TATCGACAAA AAGAAAGTAG AAACAAGCGA GTCAAAAGCA
    CGGGATCTGG TGAAAAAGTT GGACCTAGAG CTTACCGCAC GCCACGAACT CCAGGTGGAA
    ATGAAAGCCA AAGAGAGCGA CTTTGAGCTG AAAATAACAG AGCTGCAGGG GGAGAAGGAA
    TCTTTAGATG CAGAGAAGCA AGAAATCACT GCTGACAAGC AAAAAATGGA GACAGAACTC
    TCCCAACTTG TAGAGCAGGT TGAAAAATTG TCAAAGGAAC TTGAAGCTAC CAAAAAAGAA
    ATGGCATCAA TTTCCACATC TGCTGTTTTA ATCCCTGTAC CTTCTGCCTC TGGAAATGTG
    CCACCTCCAC CACCCCCTCC TCCTCCACTT CCAGGTGCAA CTGGTATTCC TCCACCTCCC
    CCACTTCCAG GTGGCTCTGG TATTCCTCCA CCACCCCCAC TTCCAGGTGG CTCTGGTATT
    GCTCCACCAC TTCCGGCTGG CTTTGGTATT CCTCCACCTC CCCCACTTCC GGGTGGCTTT
    GGTATTCCTC CACCTCCACC ACTTCCCGGT GGCTCTGGTA TTCCTCCACC TCCCCCACTT
    CCGGGTGGCT CTGGTATTCC TCCACCTCCC CCACTTCCGG GTGGCTCTGG TATTCCTCCA
    CCTCCCCCAC TTCCGGGTGG CTCTGGTATT CCTCCCCCAC CACCATTTCC GGGTGGCCCT
    GGAATTCCTC CCCCACCGCC ATTTCCAGGA GGCTTTGGAA TTCCTCCTCC TCCTCCTCCA
    TTTCCAGGTG GCCCTGGAAT TCCTCCTCCT CCTCCATTCC CTGGTGGCCC TGGGGTTCCA
    CCTCCACCTC CAGCATTTGG AGGTGGTGTT CCTCCACCAC CAGGACTTGG AGGCTGGGGG
    GCACCACCAC CCTTACCATT TGGCTTGGCT CCTAAGAAGA ATTATAAGCC AGAAGTGCAA
    CTGAAGCGAC CCAACTGGTC AAAGATCACT CCTGCTGAGC TTTCAGAGAA ATCGTTTTGG
    ACTCAGACAA AAGAAGATCG TTTTGAAAAA GAAGAGCTGT TTGCAAAAAT AACCCTCACT
    TTTTCTTCAC AGATCAAGTC TTCTAAAGCA AAAAAAGCAG AAGATGGTGA TGACAAAAAA
    GCAGCCCAGA AGAAAAAGAC AAAGGAGCTC AAAGTCTTGG ATTCCAAGAC AGCCCAAAAC
    CTATCAATCT TCCTTGGGTC GTTCCGCATG CCATACGAAG AGATCAAAAA TGTGATCCTA
    CAGGTCAATG AGGAAGTGCT GACTGAATCT ATGGTTTCAA ATCTGATCAA GCAGTTACCA
    GAACCAGATC AGTTACAGAT GTTAACAGAG TTCAAGAATG AGTACAAGGA TTTGGCAGAA
    CCAGAGCAGT TTGGAGTAGT GATGTGCAGT GTCCCCCGTT TGCGGCCACG ACTAAATGCC
    ATTCTCTTTA AACTTCTTTT TAATGAGCAA ATTGAAAATA TCAAGCCAGA CATTGTGTCA
    GTTACAGCTG CTTGCGAAGA AGTGCAAAAG TGCCAAAGCT TTGCTAGTCT CCTGGAAATC
    ACACTGCTGG TTGGGAACTT CATGAACGCA GGATCAAGGA ACGCTGATGC TTATGGCTTT
    GACATTAGCT TTCTCTGCAA GTTGAAAGAT ACCAAATCAG CGGACCAGAA GACCACACTG
    CTGCATTTCC TTGTTGAGAC TTGTGAGAAT GACTACCCTG ATGTACTGAA GTTTCCAGAT
    GAGATGGTTC ATGTAGAAAA GGCCAGTAGA GTGTCAGCTG AAACATTGCA AAAGAACCTG
    GATCAAATGA AAAAGCAGAT TGCAGACCTT CAAAGGGACA TTGATAGCTT CCCACCAACA
    GAAGATGAGA AGGATAAGTT TGTAGAAAAA ATGACAATAT TTGTCAAAGA AGCTCAAGAA
    CAGTATACTA AGCTTTGCAT GATGCACGAA AACATGCTGT CATTGTTCCA AGATTTGGGG
    AAATACTTTG TGTTTGACCC CAAGAAAACA TCCATTGAAG AGTTCTTCGC AGACCTGCAG
    AATTTCCGGA ACATGTTCTT GCACGCTCTG AAAGAGAACC AGAAGAGACG GGAAGCAGAA
    GAAAAGATGA AAAGAGCCAA ACTTGCCAAA GAGAAGGCAG AGAAGGAACG GTTAGAGAAG
    CAGCAGAAGA AAGAACAACT GATTGATATG AATGCAGAGG GTGACGAAAC TGGCGTAATG
    GACAGCCTGC TTGAAGCTTT GAATTCGGGA GCTGCATTCA GAAGGAAAAG GGGTCCTCGG
    CAAGACTCTC TCCTGTCCCT CAGTGAGGAG GAGGAGCAGG CAGCGGACTT GCGAACAGAA
    AGGCCGGCTG TGCCGTTACC TCACTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815149.2
    CDS208..3957
    Translation

    Target ORF information:

    RefSeq Version XM_012959695.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959695.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
    AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA
    TTTACCTTTA AGCGGCTGAT GGTAGATGAG CTTGAACGAT TTACCAGTAT ACGGATGAAG
    AAAGAAAAGG AAAAGCCTTA TCCATCACAC AGAAACTCCA CCAACTTCCC AGATAACTCA
    AACCCCCTCC AAACCTTGCA GGAACTCGAA GATGATGTTG TGCTTCAGCT GTTTGAAAAA
    ATGCTGGATG ATATGAACCT CAATCCTGAG AAGCAGCAAC CGCTACGAGA AAAGGATATA
    ATGATAAAAA GAGAGATGGT TTCCCAATAT CTTCACACAT CCAAAGCTGG CATGAGCAAA
    AAGGAGAGTT CTCGTTCTGC ATTAATGTAC ATTCAAGACT TAAAATCCGG ACTAGGAGAC
    AATTCCCTCC TTTTGTGCTT AGAGTCACTA AGAGTATCTT TAAATAACAA TCCTGTCAGC
    TGGGTGCAGA ATTTTGGTGC AGAGGGTCTT TCGTGCCTTT TAGAAATTCT CAAAAGAATA
    CAGGATGAGC AAGCATCCTT GCGGGATGTT AGAAGCGAGC ATGAGATCAT CAGATGTCTG
    AAAGCTTTTA TGAATAACAA TTTTGGAATT AAGGCAATGC TGGGCACAGA AGATGGCATT
    TTGCTGTTAG CGAGAGCAGT AGATCGATCT GTCCCTGCAA TGATGATTGA TTCCTTGAAG
    CTGCTTTCTG CCCTGTGCAT CCTGCCACAA CCAGAGGACA TGCATGAGCG TGTTCTAGAA
    GCCCTTACGG AGAGAGCAGA GATGGAGGAA ATGGAACGAT TTAAACCTCT TCTGGATGGG
    TTAAACATTG CAAGTCCTGT GGCCTTGAAG GTGAGCTGTA TGCAGCTAAT CAATGCAGTG
    ATCAGCCAAG GAGAAGAGCT TGATTACAGA GTTCACATCC GAAGTGAATT GATGCGCTCG
    GGACTGGCCA AACTCCTAAA GGAACTACGT AATACAGAAA ATGAAGACCT GAAGGTACAA
    CTTGGTATTT TTGATGAGCA TTCTGAACTT GATTCTGATG ACTTGCGAGG ACGTTTGGAC
    GACATCCGCA TTGAAATGGA TGACTTCAGT GACGTCTTCC AAGTTCTTCT GAACACTGTC
    AAGGACTCCT CTGCAGAACC ATTATTTCTT TCCATCCTGC AGCATCTTCT TCTAATTAGA
    AATGATTATG ATGCCAGGCC GCAGTACTAT AAACTGATAG ATGAAGTTAT ATCACAGATA
    ATCCTTCAAA AAAATGGGGC TGATCCAGAT TTCAAATGCA AGCATGTAAA CATAGAAATC
    GAAAATCTGA TAGATCATTT TATCGACAAA AAGAAAGTAG AAACAAGCGA GTCAAAAGCA
    CGGGATCTGG TGAAAAAGTT GGACCTAGAG CTTACCGCAC GCCACGAACT CCAGGTGGAA
    ATGAAAGCCA AAGAGAGCGA CTTTGAGCTG AAAATAACAG AGCTGCAGGG GGAGAAGGAA
    TCTTTAGATG CAGAGAAGCA AGAAATCACT GCTGACAAGC AAAAAATGGA GACAGAACTC
    TCCCAACTTG TAGAGCAGGT TGAAAAATTG TCAAAGGAAC TTGAAGCTAC CAAAAAAGAA
    ATGGCATCAA TTTCCACATC TGCTGTTTTA ATCCCTGTAC CTTCTGCCTC TGGAAATGTG
    CCACCTCCAC CACCCCCTCC TCCTCCACTT CCAGGTGCAA CTGGTATTCC TCCACCTCCC
    CCACTTCCAG GTGGCTCTGG TATTCCTCCA CCACCCCCAC TTCCAGGTGG CTCTGGTATT
    GCTCCACCAC TTCCGGCTGG CTTTGGTATT CCTCCACCTC CCCCACTTCC GGGTGGCTTT
    GGTATTCCTC CACCTCCACC ACTTCCCGGT GGCTCTGGTA TTCCTCCACC TCCCCCACTT
    CCGGGTGGCT CTGGTATTCC TCCACCTCCC CCACTTCCGG GTGGCTCTGG TATTCCTCCA
    CCTCCCCCAC TTCCGGGTGG CTCTGGTATT CCTCCCCCAC CACCATTTCC GGGTGGCCCT
    GGAATTCCTC CCCCACCGCC ATTTCCAGGA GGCTTTGGAA TTCCTCCTCC TCCTCCTCCA
    TTTCCAGGTG GCCCTGGAAT TCCTCCTCCT CCTCCATTCC CTGGTGGCCC TGGGGTTCCA
    CCTCCACCTC CAGCATTTGG AGGTGGTGTT CCTCCACCAC CAGGACTTGG AGGCTGGGGG
    GCACCACCAC CCTTACCATT TGGCTTGGCT CCTAAGAAGA ATTATAAGCC AGAAGTGCAA
    CTGAAGCGAC CCAACTGGTC AAAGATCACT CCTGCTGAGC TTTCAGAGAA ATCGTTTTGG
    ACTCAGACAA AAGAAGATCG TTTTGAAAAA GAAGAGCTGT TTGCAAAAAT AACCCTCACT
    TTTTCTTCAC AGATCAAGTC TTCTAAAGCA AAAAAAGCAG AAGATGGTGA TGACAAAAAA
    GCAGCCCAGA AGAAAAAGAC AAAGGAGCTC AAAGTCTTGG ATTCCAAGAC AGCCCAAAAC
    CTATCAATCT TCCTTGGGTC GTTCCGCATG CCATACGAAG AGATCAAAAA TGTGATCCTA
    CAGGTCAATG AGGAAGTGCT GACTGAATCT ATGGTTTCAA ATCTGATCAA GCAGTTACCA
    GAACCAGATC AGTTACAGAT GTTAACAGAG TTCAAGAATG AGTACAAGGA TTTGGCAGAA
    CCAGAGCAGT TTGGAGTAGT GATGTGCAGT GTCCCCCGTT TGCGGCCACG ACTAAATGCC
    ATTCTCTTTA AACTTCTTTT TAATGAGCAA ATTGAAAATA TCAAGCCAGA CATTGTGTCA
    GTTACAGCTG CTTGCGAAGA AGTGCAAAAG TGCCAAAGCT TTGCTAGTCT CCTGGAAATC
    ACACTGCTGG TTGGGAACTT CATGAACGCA GGATCAAGGA ACGCTGATGC TTATGGCTTT
    GACATTAGCT TTCTCTGCAA GTTGAAAGAT ACCAAATCAG CGGACCAGAA GACCACACTG
    CTGCATTTCC TTGTTGAGAC TTGTGAGAAT GACTACCCTG ATGTACTGAA GTTTCCAGAT
    GAGATGGTTC ATGTAGAAAA GGCCAGTAGA GTGTCAGCTG AAACATTGCA AAAGAACCTG
    GATCAAATGA AAAAGCAGAT TGCAGACCTT CAAAGGGACA TTGATAGCTT CCCACCAACA
    GAAGATGAGA AGGATAAGTT TGTAGAAAAA ATGACAATAT TTGTCAAAGA AGCTCAAGAA
    CAGTATACTA AGCTTTGCAT GATGCACGAA AACATGCTGT CATTGTTCCA AGATTTGGGG
    AAATACTTTG TGTTTGACCC CAAGAAAACA TCCATTGAAG AGTTCTTCGC AGACCTGCAG
    AATTTCCGGA ACATGTTCTT GCACGCTCTG AAAGAGAACC AGAAGAGACG GGAAGCAGAA
    GAAAAGATGA AAAGAGCCAA ACTTGCCAAA GAGAAGGCAG AGAAGGAACG GTTAGAGAAG
    CAGCAGAAGA AAGAACAACT GATTGATATG AATGCAGAGG GTGACGAAAC TGGCGTAATG
    GACAGCCTGC TTGAAGCTTT GAATTCGGGA GCTGCATTCA GAAGGAAAAG GGGTCCTCGG
    CAAGACTCTC TCCTGTCCCT CAGTGAGGAG GAGGAGCAGG CAGCGGACTT GCGAACAGAA
    AGGCCGGCTG TGCCGTTACC TCACTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa28573
    Clone ID Related Accession (Same CDS sequence) XM_012959693.2
    Accession Version XM_012959693.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3909bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein diaphanous homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959693.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
    AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA
    TTTACCTTTA AGCGGCTGAT GGTAGATGAG CTTGAACGAT TTACCAGTAT ACGGATGAAG
    AAAGAAAAGG AAAAGCCTTA TCCATCACAC AGAAACTCCA CCAACTTCCC AGATAACTCA
    AACCCCCTCC AAACCTTGCA GGAACTCGAA GATGATGTTG TGCTTCAGCT GTTTGAAAAA
    ATGCTGGATG ATATGAACCT CAATCCTGAG AAGCAGCAAC CGCTACGAGA AAAGGATATA
    ATGATAAAAA GAGAGATGGT TTCCCAATAT CTTCACACAT CCAAAGCTGG CATGAGCAAA
    AAGGAGAGTT CTCGTTCTGC ATTAATGTAC ATTCAAGACT TAAAATCCGG ACTAGGAGAC
    AATTCCCTCC TTTTGTGCTT AGAGTCACTA AGAGTATCTT TAAATAACAA TCCTGTCAGC
    TGGGTGCAGA ATTTTGGTGC AGAGGGTCTT TCGTGCCTTT TAGAAATTCT CAAAAGAATA
    CAGGATGAGC AAGCATCCTT GCGGGATGTT AGAAGCGAGC ATGAGATCAT CAGATGTCTG
    AAAGCTTTTA TGAATAACAA TTTTGGAATT AAGGCAATGC TGGGCACAGA AGATGGCATT
    TTGCTGTTAG CGAGAGCAGT AGATCGATCT GTCCCTGCAA TGATGATTGA TTCCTTGAAG
    CTGCTTTCTG CCCTGTGCAT CCTGCCACAA CCAGAGGACA TGCATGAGCG TGTTCTAGAA
    GCCCTTACGG AGAGAGCAGA GATGGAGGAA ATGGAACGAT TTAAACCTCT TCTGGATGGG
    TTAAACATTG CAAGTCCTGT GGCCTTGAAG GTGAGCTGTA TGCAGCTAAT CAATGCAGTG
    ATCAGCCAAG GAGAAGAGCT TGATTACAGA GTTCACATCC GAAGTGAATT GATGCGCTCG
    GGACTGGCCA AACTCCTAAA GGAACTACGT AATACAGAAA ATGAAGACCT GAAGGTACAA
    CTTGGTATTT TTGATGAGCA TTCTGAACTT GATTCTGATG ACTTGCGAGG ACGTTTGGAC
    GACATCCGCA TTGAAATGGA TGACTTCAGT GACGTCTTCC AAGTTCTTCT GAACACTGTC
    AAGGACTCCT CTGCAGAACC ATTATTTCTT TCCATCCTGC AGCATCTTCT TCTAATTAGA
    AATGATTATG ATGCCAGGCC GCAGTACTAT AAACTGATAG ATGAAGTTAT ATCACAGATA
    ATCCTTCAAA AAAATGGGGC TGATCCAGAT TTCAAATGCA AGCATGTAAA CATAGAAATC
    GAAAATCTGA TAGATCATTT TATCGACAAA AAGAAAGTAG AAACAAGCGA GTCAAAAGCA
    CGGGATCTGG TGAAAAAGTT GGACCTAGAG CTTACCGCAC GCCACGAACT CCAGGTGGAA
    ATGAAAGCCA AAGAGAGCGA CTTTGAGCTG AAAATAACAG AGCTGCAGGG GGAGAAGGAA
    TCTTTAGATG CAGAGAAGCA AGAAATCACT GCTGACAAGC AAAAAATGGA GACAGAACTC
    TCCCAACTTG TAGAGCAGGT TGAAAAATTG TCAAAGGAAC TTGAAGCTAC CAAAAAAGAA
    ATGGCATCAA TTTCCACATC TGCTGTTTTA ATCCCTGTAC CTTCTGCCTC TGGAAATGTG
    CCACCTCCAC CACCCCCTCC TCCTCCACTT CCAGGTGCAA CTGGTATTCC TCCACCCCCC
    CCACTTCCAG GTGGCTCTGG TATTCCTCCA CCTCCCCCAC TTCCAGGTGG CTCTGGTATT
    CCTCCACCAC CCCCACTTCC AGGTGGCTCT GGTATTTCTC CACCACTTCC GGGTGGCTTT
    GGTATTCCTC CACCTCCCCC ACTTCCGGGT GGCTTTGGTA TTCCTCCACC TCCACCACTT
    CCCGGTGGCT CTGGTATTCC TCCACCTCCC CCACTTCCGG GTGGCTCTGG TATTCCTCCA
    CCTCCCCCAC TTCCGGGTGG CTCTGGTATT CCTCCACCTC CCCCACTTCC GGGTGGCTCT
    GGTATTCCTC CCCCACCACC ACTTCCGGGT GGCTCTGGTA TTCCTCCCCC ACCACCATTT
    CCGGGTGGCC CTGGAATTCC TCCCCCACCG CCATTTCCAG GAGGCTTTGG AATTCCTCCT
    CCTCCTCCTC CATTTCCAGG TGGCCCTGGA ATTCCTCCTC CTCCTCCATT CCCTGGTGGC
    CCTGGGGTTC CACCTCCACC TCCAGCATTT GGAGGTGGTG TTCCTCCACC ACCAGGACTT
    GGAGGCTGGG GGGCACCACC ACCCTTACCA TTTGGCTTGG CTCCTAAGAA GAATTATAAG
    CCAGAAGTGC AACTGAAGCG ACCCAACTGG TCAAAGATCA CTCCTGCTGA GCTTTCAGAG
    AAATCGTTTT GGACTCAGAC AAAAGAAGAT CGTTTTGAAA AAGAAGAGCT GTTTGCAAAA
    ATAACCCTCA CTTTTTCTTC ACAGATCAAG TCTTCTAAAG CAAAAAAAGC AGAAGATGGT
    GATGACAAAA AAGCAGCCCA GAAGAAAAAG ACAAAGGAGC TCAAAGTCTT GGATTCCAAG
    ACAGCCCAAA ACCTATCAAT CTTCCTTGGG TCGTTCCGCA TGCCATACGA AGAGATCAAA
    AATGTGATCC TACAGGTCAA TGAGGAAGTG CTGACTGAAT CTATGGTTTC AAATCTGATC
    AAGCAGTTAC CAGAACCAGA TCAGTTACAG ATGTTAACAG AGTTCAAGAA TGAGTACAAG
    GATTTGGCAG AACCAGAGCA GTTTGGAGTA GTGATGTGCA GTGTCCCCCG TTTGCGGCCA
    CGACTAAATG CCATTCTCTT TAAACTTCTT TTTAATGAGC AAATTGAAAA TATCAAGCCA
    GACATTGTGT CAGTTACAGC TGCTTGCGAA GAAGTGCAAA AGTGCCAAAG CTTTGCTAGT
    CTCCTGGAAA TCACACTGCT GGTTGGGAAC TTCATGAACG CAGGATCAAG GAACGCTGAT
    GCTTATGGCT TTGACATTAG CTTTCTCTGC AAGTTGAAAG ATACCAAATC AGCGGACCAG
    AAGACCACAC TGCTGCATTT CCTTGTTGAG ACTTGTGAGA ATGACTACCC TGATGTACTG
    AAGTTTCCAG ATGAGATGGT TCATGTAGAA AAGGCCAGTA GAGTGTCAGC TGAAACATTG
    CAAAAGAACC TGGATCAAAT GAAAAAGCAG ATTGCAGACC TTCAAAGGGA CATTGATAGC
    TTCCCACCAA CAGAAGATGA GAAGGATAAG TTTGTAGAAA AAATGACAAT ATTTGTCAAA
    GAAGCTCAAG AACAGTATAC TAAGCTTTGC ATGATGCACG AAAACATGCT GTCATTGTTC
    CAAGATTTGG GGAAATACTT TGTGTTTGAC CCCAAGAAAA CATCCATTGA AGAGTTCTTC
    GCAGACCTGC AGAATTTCCG GAACATGTTC TTGCACGCTC TGAAAGAGAA CCAGAAGAGA
    CGGGAAGCAG AAGAAAAGAT GAAAAGAGCC AAACTTGCCA AAGAGAAGGC AGAGAAGGAA
    CGGTTAGAGA AGCAGCAGAA GAAAGAACAA CTGATTGATA TGAATGCAGA GGGTGACGAA
    ACTGGCGTAA TGGACAGCCT GCTTGAAGCT TTGAATTCGG GAGCTGCATT CAGAAGGAAA
    AGGGGTCCTC GGCAAGCGAA CAGAAAGGCC GGCTGTGCCG TTACCTCACT GCTAGCATCT
    GAGCTCACTA AAGAAGATGC AATGACCTCA GTGCCCACTA AGAAGAAAAA ACCCGCTGAG
    ACGGATTTGA TGGAAACAGA TGTAGGACTG CACGAAGATG CCCAGGATAT GCTTACGCGA
    GCAAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815147.1
    CDS575..4483
    Translation

    Target ORF information:

    RefSeq Version XM_012959693.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959693.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
    AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA
    TTTACCTTTA AGCGGCTGAT GGTAGATGAG CTTGAACGAT TTACCAGTAT ACGGATGAAG
    AAAGAAAAGG AAAAGCCTTA TCCATCACAC AGAAACTCCA CCAACTTCCC AGATAACTCA
    AACCCCCTCC AAACCTTGCA GGAACTCGAA GATGATGTTG TGCTTCAGCT GTTTGAAAAA
    ATGCTGGATG ATATGAACCT CAATCCTGAG AAGCAGCAAC CGCTACGAGA AAAGGATATA
    ATGATAAAAA GAGAGATGGT TTCCCAATAT CTTCACACAT CCAAAGCTGG CATGAGCAAA
    AAGGAGAGTT CTCGTTCTGC ATTAATGTAC ATTCAAGACT TAAAATCCGG ACTAGGAGAC
    AATTCCCTCC TTTTGTGCTT AGAGTCACTA AGAGTATCTT TAAATAACAA TCCTGTCAGC
    TGGGTGCAGA ATTTTGGTGC AGAGGGTCTT TCGTGCCTTT TAGAAATTCT CAAAAGAATA
    CAGGATGAGC AAGCATCCTT GCGGGATGTT AGAAGCGAGC ATGAGATCAT CAGATGTCTG
    AAAGCTTTTA TGAATAACAA TTTTGGAATT AAGGCAATGC TGGGCACAGA AGATGGCATT
    TTGCTGTTAG CGAGAGCAGT AGATCGATCT GTCCCTGCAA TGATGATTGA TTCCTTGAAG
    CTGCTTTCTG CCCTGTGCAT CCTGCCACAA CCAGAGGACA TGCATGAGCG TGTTCTAGAA
    GCCCTTACGG AGAGAGCAGA GATGGAGGAA ATGGAACGAT TTAAACCTCT TCTGGATGGG
    TTAAACATTG CAAGTCCTGT GGCCTTGAAG GTGAGCTGTA TGCAGCTAAT CAATGCAGTG
    ATCAGCCAAG GAGAAGAGCT TGATTACAGA GTTCACATCC GAAGTGAATT GATGCGCTCG
    GGACTGGCCA AACTCCTAAA GGAACTACGT AATACAGAAA ATGAAGACCT GAAGGTACAA
    CTTGGTATTT TTGATGAGCA TTCTGAACTT GATTCTGATG ACTTGCGAGG ACGTTTGGAC
    GACATCCGCA TTGAAATGGA TGACTTCAGT GACGTCTTCC AAGTTCTTCT GAACACTGTC
    AAGGACTCCT CTGCAGAACC ATTATTTCTT TCCATCCTGC AGCATCTTCT TCTAATTAGA
    AATGATTATG ATGCCAGGCC GCAGTACTAT AAACTGATAG ATGAAGTTAT ATCACAGATA
    ATCCTTCAAA AAAATGGGGC TGATCCAGAT TTCAAATGCA AGCATGTAAA CATAGAAATC
    GAAAATCTGA TAGATCATTT TATCGACAAA AAGAAAGTAG AAACAAGCGA GTCAAAAGCA
    CGGGATCTGG TGAAAAAGTT GGACCTAGAG CTTACCGCAC GCCACGAACT CCAGGTGGAA
    ATGAAAGCCA AAGAGAGCGA CTTTGAGCTG AAAATAACAG AGCTGCAGGG GGAGAAGGAA
    TCTTTAGATG CAGAGAAGCA AGAAATCACT GCTGACAAGC AAAAAATGGA GACAGAACTC
    TCCCAACTTG TAGAGCAGGT TGAAAAATTG TCAAAGGAAC TTGAAGCTAC CAAAAAAGAA
    ATGGCATCAA TTTCCACATC TGCTGTTTTA ATCCCTGTAC CTTCTGCCTC TGGAAATGTG
    CCACCTCCAC CACCCCCTCC TCCTCCACTT CCAGGTGCAA CTGGTATTCC TCCACCCCCC
    CCACTTCCAG GTGGCTCTGG TATTCCTCCA CCTCCCCCAC TTCCAGGTGG CTCTGGTATT
    CCTCCACCAC CCCCACTTCC AGGTGGCTCT GGTATTTCTC CACCACTTCC GGGTGGCTTT
    GGTATTCCTC CACCTCCCCC ACTTCCGGGT GGCTTTGGTA TTCCTCCACC TCCACCACTT
    CCCGGTGGCT CTGGTATTCC TCCACCTCCC CCACTTCCGG GTGGCTCTGG TATTCCTCCA
    CCTCCCCCAC TTCCGGGTGG CTCTGGTATT CCTCCACCTC CCCCACTTCC GGGTGGCTCT
    GGTATTCCTC CCCCACCACC ACTTCCGGGT GGCTCTGGTA TTCCTCCCCC ACCACCATTT
    CCGGGTGGCC CTGGAATTCC TCCCCCACCG CCATTTCCAG GAGGCTTTGG AATTCCTCCT
    CCTCCTCCTC CATTTCCAGG TGGCCCTGGA ATTCCTCCTC CTCCTCCATT CCCTGGTGGC
    CCTGGGGTTC CACCTCCACC TCCAGCATTT GGAGGTGGTG TTCCTCCACC ACCAGGACTT
    GGAGGCTGGG GGGCACCACC ACCCTTACCA TTTGGCTTGG CTCCTAAGAA GAATTATAAG
    CCAGAAGTGC AACTGAAGCG ACCCAACTGG TCAAAGATCA CTCCTGCTGA GCTTTCAGAG
    AAATCGTTTT GGACTCAGAC AAAAGAAGAT CGTTTTGAAA AAGAAGAGCT GTTTGCAAAA
    ATAACCCTCA CTTTTTCTTC ACAGATCAAG TCTTCTAAAG CAAAAAAAGC AGAAGATGGT
    GATGACAAAA AAGCAGCCCA GAAGAAAAAG ACAAAGGAGC TCAAAGTCTT GGATTCCAAG
    ACAGCCCAAA ACCTATCAAT CTTCCTTGGG TCGTTCCGCA TGCCATACGA AGAGATCAAA
    AATGTGATCC TACAGGTCAA TGAGGAAGTG CTGACTGAAT CTATGGTTTC AAATCTGATC
    AAGCAGTTAC CAGAACCAGA TCAGTTACAG ATGTTAACAG AGTTCAAGAA TGAGTACAAG
    GATTTGGCAG AACCAGAGCA GTTTGGAGTA GTGATGTGCA GTGTCCCCCG TTTGCGGCCA
    CGACTAAATG CCATTCTCTT TAAACTTCTT TTTAATGAGC AAATTGAAAA TATCAAGCCA
    GACATTGTGT CAGTTACAGC TGCTTGCGAA GAAGTGCAAA AGTGCCAAAG CTTTGCTAGT
    CTCCTGGAAA TCACACTGCT GGTTGGGAAC TTCATGAACG CAGGATCAAG GAACGCTGAT
    GCTTATGGCT TTGACATTAG CTTTCTCTGC AAGTTGAAAG ATACCAAATC AGCGGACCAG
    AAGACCACAC TGCTGCATTT CCTTGTTGAG ACTTGTGAGA ATGACTACCC TGATGTACTG
    AAGTTTCCAG ATGAGATGGT TCATGTAGAA AAGGCCAGTA GAGTGTCAGC TGAAACATTG
    CAAAAGAACC TGGATCAAAT GAAAAAGCAG ATTGCAGACC TTCAAAGGGA CATTGATAGC
    TTCCCACCAA CAGAAGATGA GAAGGATAAG TTTGTAGAAA AAATGACAAT ATTTGTCAAA
    GAAGCTCAAG AACAGTATAC TAAGCTTTGC ATGATGCACG AAAACATGCT GTCATTGTTC
    CAAGATTTGG GGAAATACTT TGTGTTTGAC CCCAAGAAAA CATCCATTGA AGAGTTCTTC
    GCAGACCTGC AGAATTTCCG GAACATGTTC TTGCACGCTC TGAAAGAGAA CCAGAAGAGA
    CGGGAAGCAG AAGAAAAGAT GAAAAGAGCC AAACTTGCCA AAGAGAAGGC AGAGAAGGAA
    CGGTTAGAGA AGCAGCAGAA GAAAGAACAA CTGATTGATA TGAATGCAGA GGGTGACGAA
    ACTGGCGTAA TGGACAGCCT GCTTGAAGCT TTGAATTCGG GAGCTGCATT CAGAAGGAAA
    AGGGGTCCTC GGCAAGCGAA CAGAAAGGCC GGCTGTGCCG TTACCTCACT GCTAGCATCT
    GAGCTCACTA AAGAAGATGC AATGACCTCA GTGCCCACTA AGAAGAAAAA ACCCGCTGAG
    ACGGATTTGA TGGAAACAGA TGTAGGACTG CACGAAGATG CCCAGGATAT GCTTACGCGA
    GCAAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa28574
    Clone ID Related Accession (Same CDS sequence) XM_012959694.2
    Accession Version XM_012959694.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3882bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein diaphanous homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959694.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
    AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA
    TTTCTTGAAC GATTTACCAG TATACGGATG AAGAAAGAAA AGGAAAAGCC TTATCCATCA
    CACAGAAACT CCACCAACTT CCCAGATAAC TCAAACCCCC TCCAAACCTT GCAGGAACTC
    GAAGATGATG TTGTGCTTCA GCTGTTTGAA AAAATGCTGG ATGATATGAA CCTCAATCCT
    GAGAAGCAGC AACCGCTACG AGAAAAGGAT ATAATGATAA AAAGAGAGAT GGTTTCCCAA
    TATCTTCACA CATCCAAAGC TGGCATGAGC AAAAAGGAGA GTTCTCGTTC TGCATTAATG
    TACATTCAAG ACTTAAAATC CGGACTAGGA GACAATTCCC TCCTTTTGTG CTTAGAGTCA
    CTAAGAGTAT CTTTAAATAA CAATCCTGTC AGCTGGGTGC AGAATTTTGG TGCAGAGGGT
    CTTTCGTGCC TTTTAGAAAT TCTCAAAAGA ATACAGGATG AGCAAGCATC CTTGCGGGAT
    GTTAGAAGCG AGCATGAGAT CATCAGATGT CTGAAAGCTT TTATGAATAA CAATTTTGGA
    ATTAAGGCAA TGCTGGGCAC AGAAGATGGC ATTTTGCTGT TAGCGAGAGC AGTAGATCGA
    TCTGTCCCTG CAATGATGAT TGATTCCTTG AAGCTGCTTT CTGCCCTGTG CATCCTGCCA
    CAACCAGAGG ACATGCATGA GCGTGTTCTA GAAGCCCTTA CGGAGAGAGC AGAGATGGAG
    GAAATGGAAC GATTTAAACC TCTTCTGGAT GGGTTAAACA TTGCAAGTCC TGTGGCCTTG
    AAGGTGAGCT GTATGCAGCT AATCAATGCA GTGATCAGCC AAGGAGAAGA GCTTGATTAC
    AGAGTTCACA TCCGAAGTGA ATTGATGCGC TCGGGACTGG CCAAACTCCT AAAGGAACTA
    CGTAATACAG AAAATGAAGA CCTGAAGGTA CAACTTGGTA TTTTTGATGA GCATTCTGAA
    CTTGATTCTG ATGACTTGCG AGGACGTTTG GACGACATCC GCATTGAAAT GGATGACTTC
    AGTGACGTCT TCCAAGTTCT TCTGAACACT GTCAAGGACT CCTCTGCAGA ACCATTATTT
    CTTTCCATCC TGCAGCATCT TCTTCTAATT AGAAATGATT ATGATGCCAG GCCGCAGTAC
    TATAAACTGA TAGATGAAGT TATATCACAG ATAATCCTTC AAAAAAATGG GGCTGATCCA
    GATTTCAAAT GCAAGCATGT AAACATAGAA ATCGAAAATC TGATAGATCA TTTTATCGAC
    AAAAAGAAAG TAGAAACAAG CGAGTCAAAA GCACGGGATC TGGTGAAAAA GTTGGACCTA
    GAGCTTACCG CACGCCACGA ACTCCAGGTG GAAATGAAAG CCAAAGAGAG CGACTTTGAG
    CTGAAAATAA CAGAGCTGCA GGGGGAGAAG GAATCTTTAG ATGCAGAGAA GCAAGAAATC
    ACTGCTGACA AGCAAAAAAT GGAGACAGAA CTCTCCCAAC TTGTAGAGCA GGTTGAAAAA
    TTGTCAAAGG AACTTGAAGC TACCAAAAAA GAAATGGCAT CAATTTCCAC ATCTGCTGTT
    TTAATCCCTG TACCTTCTGC CTCTGGAAAT GTGCCACCTC CACCACCCCC TCCTCCTCCA
    CTTCCAGGTG CAACTGGTAT TCCTCCACCC CCCCCACTTC CAGGTGGCTC TGGTATTCCT
    CCACCTCCCC CACTTCCAGG TGGCTCTGGT ATTCCTCCAC CACCCCCACT TCCAGGTGGC
    TCTGGTATTT CTCCACCACT TCCGGGTGGC TTTGGTATTC CTCCACCTCC CCCACTTCCG
    GGTGGCTTTG GTATTCCTCC ACCTCCACCA CTTCCCGGTG GCTCTGGTAT TCCTCCACCT
    CCCCCACTTC CGGGTGGCTC TGGTATTCCT CCACCTCCCC CACTTCCGGG TGGCTCTGGT
    ATTCCTCCAC CTCCCCCACT TCCGGGTGGC TCTGGTATTC CTCCCCCACC ACCACTTCCG
    GGTGGCTCTG GTATTCCTCC CCCACCACCA TTTCCGGGTG GCCCTGGAAT TCCTCCCCCA
    CCGCCATTTC CAGGAGGCTT TGGAATTCCT CCTCCTCCTC CTCCATTTCC AGGTGGCCCT
    GGAATTCCTC CTCCTCCTCC ATTCCCTGGT GGCCCTGGGG TTCCACCTCC ACCTCCAGCA
    TTTGGAGGTG GTGTTCCTCC ACCACCAGGA CTTGGAGGCT GGGGGGCACC ACCACCCTTA
    CCATTTGGCT TGGCTCCTAA GAAGAATTAT AAGCCAGAAG TGCAACTGAA GCGACCCAAC
    TGGTCAAAGA TCACTCCTGC TGAGCTTTCA GAGAAATCGT TTTGGACTCA GACAAAAGAA
    GATCGTTTTG AAAAAGAAGA GCTGTTTGCA AAAATAACCC TCACTTTTTC TTCACAGATC
    AAGTCTTCTA AAGCAAAAAA AGCAGAAGAT GGTGATGACA AAAAAGCAGC CCAGAAGAAA
    AAGACAAAGG AGCTCAAAGT CTTGGATTCC AAGACAGCCC AAAACCTATC AATCTTCCTT
    GGGTCGTTCC GCATGCCATA CGAAGAGATC AAAAATGTGA TCCTACAGGT CAATGAGGAA
    GTGCTGACTG AATCTATGGT TTCAAATCTG ATCAAGCAGT TACCAGAACC AGATCAGTTA
    CAGATGTTAA CAGAGTTCAA GAATGAGTAC AAGGATTTGG CAGAACCAGA GCAGTTTGGA
    GTAGTGATGT GCAGTGTCCC CCGTTTGCGG CCACGACTAA ATGCCATTCT CTTTAAACTT
    CTTTTTAATG AGCAAATTGA AAATATCAAG CCAGACATTG TGTCAGTTAC AGCTGCTTGC
    GAAGAAGTGC AAAAGTGCCA AAGCTTTGCT AGTCTCCTGG AAATCACACT GCTGGTTGGG
    AACTTCATGA ACGCAGGATC AAGGAACGCT GATGCTTATG GCTTTGACAT TAGCTTTCTC
    TGCAAGTTGA AAGATACCAA ATCAGCGGAC CAGAAGACCA CACTGCTGCA TTTCCTTGTT
    GAGACTTGTG AGAATGACTA CCCTGATGTA CTGAAGTTTC CAGATGAGAT GGTTCATGTA
    GAAAAGGCCA GTAGAGTGTC AGCTGAAACA TTGCAAAAGA ACCTGGATCA AATGAAAAAG
    CAGATTGCAG ACCTTCAAAG GGACATTGAT AGCTTCCCAC CAACAGAAGA TGAGAAGGAT
    AAGTTTGTAG AAAAAATGAC AATATTTGTC AAAGAAGCTC AAGAACAGTA TACTAAGCTT
    TGCATGATGC ACGAAAACAT GCTGTCATTG TTCCAAGATT TGGGGAAATA CTTTGTGTTT
    GACCCCAAGA AAACATCCAT TGAAGAGTTC TTCGCAGACC TGCAGAATTT CCGGAACATG
    TTCTTGCACG CTCTGAAAGA GAACCAGAAG AGACGGGAAG CAGAAGAAAA GATGAAAAGA
    GCCAAACTTG CCAAAGAGAA GGCAGAGAAG GAACGGTTAG AGAAGCAGCA GAAGAAAGAA
    CAACTGATTG ATATGAATGC AGAGGGTGAC GAAACTGGCG TAATGGACAG CCTGCTTGAA
    GCTTTGAATT CGGGAGCTGC ATTCAGAAGG AAAAGGGGTC CTCGGCAAGC GAACAGAAAG
    GCCGGCTGTG CCGTTACCTC ACTGCTAGCA TCTGAGCTCA CTAAAGAAGA TGCAATGACC
    TCAGTGCCCA CTAAGAAGAA AAAACCCGCT GAGACGGATT TGATGGAAAC AGATGTAGGA
    CTGCACGAAG ATGCCCAGGA TATGCTTACG CGAGCAAGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815148.1
    CDS573..4454
    Translation

    Target ORF information:

    RefSeq Version XM_012959694.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959694.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
    AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA
    TTTCTTGAAC GATTTACCAG TATACGGATG AAGAAAGAAA AGGAAAAGCC TTATCCATCA
    CACAGAAACT CCACCAACTT CCCAGATAAC TCAAACCCCC TCCAAACCTT GCAGGAACTC
    GAAGATGATG TTGTGCTTCA GCTGTTTGAA AAAATGCTGG ATGATATGAA CCTCAATCCT
    GAGAAGCAGC AACCGCTACG AGAAAAGGAT ATAATGATAA AAAGAGAGAT GGTTTCCCAA
    TATCTTCACA CATCCAAAGC TGGCATGAGC AAAAAGGAGA GTTCTCGTTC TGCATTAATG
    TACATTCAAG ACTTAAAATC CGGACTAGGA GACAATTCCC TCCTTTTGTG CTTAGAGTCA
    CTAAGAGTAT CTTTAAATAA CAATCCTGTC AGCTGGGTGC AGAATTTTGG TGCAGAGGGT
    CTTTCGTGCC TTTTAGAAAT TCTCAAAAGA ATACAGGATG AGCAAGCATC CTTGCGGGAT
    GTTAGAAGCG AGCATGAGAT CATCAGATGT CTGAAAGCTT TTATGAATAA CAATTTTGGA
    ATTAAGGCAA TGCTGGGCAC AGAAGATGGC ATTTTGCTGT TAGCGAGAGC AGTAGATCGA
    TCTGTCCCTG CAATGATGAT TGATTCCTTG AAGCTGCTTT CTGCCCTGTG CATCCTGCCA
    CAACCAGAGG ACATGCATGA GCGTGTTCTA GAAGCCCTTA CGGAGAGAGC AGAGATGGAG
    GAAATGGAAC GATTTAAACC TCTTCTGGAT GGGTTAAACA TTGCAAGTCC TGTGGCCTTG
    AAGGTGAGCT GTATGCAGCT AATCAATGCA GTGATCAGCC AAGGAGAAGA GCTTGATTAC
    AGAGTTCACA TCCGAAGTGA ATTGATGCGC TCGGGACTGG CCAAACTCCT AAAGGAACTA
    CGTAATACAG AAAATGAAGA CCTGAAGGTA CAACTTGGTA TTTTTGATGA GCATTCTGAA
    CTTGATTCTG ATGACTTGCG AGGACGTTTG GACGACATCC GCATTGAAAT GGATGACTTC
    AGTGACGTCT TCCAAGTTCT TCTGAACACT GTCAAGGACT CCTCTGCAGA ACCATTATTT
    CTTTCCATCC TGCAGCATCT TCTTCTAATT AGAAATGATT ATGATGCCAG GCCGCAGTAC
    TATAAACTGA TAGATGAAGT TATATCACAG ATAATCCTTC AAAAAAATGG GGCTGATCCA
    GATTTCAAAT GCAAGCATGT AAACATAGAA ATCGAAAATC TGATAGATCA TTTTATCGAC
    AAAAAGAAAG TAGAAACAAG CGAGTCAAAA GCACGGGATC TGGTGAAAAA GTTGGACCTA
    GAGCTTACCG CACGCCACGA ACTCCAGGTG GAAATGAAAG CCAAAGAGAG CGACTTTGAG
    CTGAAAATAA CAGAGCTGCA GGGGGAGAAG GAATCTTTAG ATGCAGAGAA GCAAGAAATC
    ACTGCTGACA AGCAAAAAAT GGAGACAGAA CTCTCCCAAC TTGTAGAGCA GGTTGAAAAA
    TTGTCAAAGG AACTTGAAGC TACCAAAAAA GAAATGGCAT CAATTTCCAC ATCTGCTGTT
    TTAATCCCTG TACCTTCTGC CTCTGGAAAT GTGCCACCTC CACCACCCCC TCCTCCTCCA
    CTTCCAGGTG CAACTGGTAT TCCTCCACCC CCCCCACTTC CAGGTGGCTC TGGTATTCCT
    CCACCTCCCC CACTTCCAGG TGGCTCTGGT ATTCCTCCAC CACCCCCACT TCCAGGTGGC
    TCTGGTATTT CTCCACCACT TCCGGGTGGC TTTGGTATTC CTCCACCTCC CCCACTTCCG
    GGTGGCTTTG GTATTCCTCC ACCTCCACCA CTTCCCGGTG GCTCTGGTAT TCCTCCACCT
    CCCCCACTTC CGGGTGGCTC TGGTATTCCT CCACCTCCCC CACTTCCGGG TGGCTCTGGT
    ATTCCTCCAC CTCCCCCACT TCCGGGTGGC TCTGGTATTC CTCCCCCACC ACCACTTCCG
    GGTGGCTCTG GTATTCCTCC CCCACCACCA TTTCCGGGTG GCCCTGGAAT TCCTCCCCCA
    CCGCCATTTC CAGGAGGCTT TGGAATTCCT CCTCCTCCTC CTCCATTTCC AGGTGGCCCT
    GGAATTCCTC CTCCTCCTCC ATTCCCTGGT GGCCCTGGGG TTCCACCTCC ACCTCCAGCA
    TTTGGAGGTG GTGTTCCTCC ACCACCAGGA CTTGGAGGCT GGGGGGCACC ACCACCCTTA
    CCATTTGGCT TGGCTCCTAA GAAGAATTAT AAGCCAGAAG TGCAACTGAA GCGACCCAAC
    TGGTCAAAGA TCACTCCTGC TGAGCTTTCA GAGAAATCGT TTTGGACTCA GACAAAAGAA
    GATCGTTTTG AAAAAGAAGA GCTGTTTGCA AAAATAACCC TCACTTTTTC TTCACAGATC
    AAGTCTTCTA AAGCAAAAAA AGCAGAAGAT GGTGATGACA AAAAAGCAGC CCAGAAGAAA
    AAGACAAAGG AGCTCAAAGT CTTGGATTCC AAGACAGCCC AAAACCTATC AATCTTCCTT
    GGGTCGTTCC GCATGCCATA CGAAGAGATC AAAAATGTGA TCCTACAGGT CAATGAGGAA
    GTGCTGACTG AATCTATGGT TTCAAATCTG ATCAAGCAGT TACCAGAACC AGATCAGTTA
    CAGATGTTAA CAGAGTTCAA GAATGAGTAC AAGGATTTGG CAGAACCAGA GCAGTTTGGA
    GTAGTGATGT GCAGTGTCCC CCGTTTGCGG CCACGACTAA ATGCCATTCT CTTTAAACTT
    CTTTTTAATG AGCAAATTGA AAATATCAAG CCAGACATTG TGTCAGTTAC AGCTGCTTGC
    GAAGAAGTGC AAAAGTGCCA AAGCTTTGCT AGTCTCCTGG AAATCACACT GCTGGTTGGG
    AACTTCATGA ACGCAGGATC AAGGAACGCT GATGCTTATG GCTTTGACAT TAGCTTTCTC
    TGCAAGTTGA AAGATACCAA ATCAGCGGAC CAGAAGACCA CACTGCTGCA TTTCCTTGTT
    GAGACTTGTG AGAATGACTA CCCTGATGTA CTGAAGTTTC CAGATGAGAT GGTTCATGTA
    GAAAAGGCCA GTAGAGTGTC AGCTGAAACA TTGCAAAAGA ACCTGGATCA AATGAAAAAG
    CAGATTGCAG ACCTTCAAAG GGACATTGAT AGCTTCCCAC CAACAGAAGA TGAGAAGGAT
    AAGTTTGTAG AAAAAATGAC AATATTTGTC AAAGAAGCTC AAGAACAGTA TACTAAGCTT
    TGCATGATGC ACGAAAACAT GCTGTCATTG TTCCAAGATT TGGGGAAATA CTTTGTGTTT
    GACCCCAAGA AAACATCCAT TGAAGAGTTC TTCGCAGACC TGCAGAATTT CCGGAACATG
    TTCTTGCACG CTCTGAAAGA GAACCAGAAG AGACGGGAAG CAGAAGAAAA GATGAAAAGA
    GCCAAACTTG CCAAAGAGAA GGCAGAGAAG GAACGGTTAG AGAAGCAGCA GAAGAAAGAA
    CAACTGATTG ATATGAATGC AGAGGGTGAC GAAACTGGCG TAATGGACAG CCTGCTTGAA
    GCTTTGAATT CGGGAGCTGC ATTCAGAAGG AAAAGGGGTC CTCGGCAAGC GAACAGAAAG
    GCCGGCTGTG CCGTTACCTC ACTGCTAGCA TCTGAGCTCA CTAAAGAAGA TGCAATGACC
    TCAGTGCCCA CTAAGAAGAA AAAACCCGCT GAGACGGATT TGATGGAAAC AGATGTAGGA
    CTGCACGAAG ATGCCCAGGA TATGCTTACG CGAGCAAGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa28575
    Clone ID Related Accession (Same CDS sequence) XM_012959695.2
    Accession Version XM_012959695.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3882bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein diaphanous homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959695.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
    AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA
    TTTACCTTTA AGCGGCTGAT GGTAGATGAG CTTGAACGAT TTACCAGTAT ACGGATGAAG
    AAAGAAAAGG AAAAGCCTTA TCCATCACAC AGAAACTCCA CCAACTTCCC AGATAACTCA
    AACCCCCTCC AAACCTTGCA GGAACTCGAA GATGATGTTG TGCTTCAGCT GTTTGAAAAA
    ATGCTGGATG ATATGAACCT CAATCCTGAG AAGCAGCAAC CGCTACGAGA AAAGGATATA
    ATGATAAAAA GAGAGATGGT TTCCCAATAT CTTCACACAT CCAAAGCTGG CATGAGCAAA
    AAGGAGAGTT CTCGTTCTGC ATTAATGTAC ATTCAAGACT TAAAATCCGG ACTAGGAGAC
    AATTCCCTCC TTTTGTGCTT AGAGTCACTA AGAGTATCTT TAAATAACAA TCCTGTCAGC
    TGGGTGCAGA ATTTTGGTGC AGAGGGTCTT TCGTGCCTTT TAGAAATTCT CAAAAGAATA
    CAGGATGAGC AAGCATCCTT GCGGGATGTT AGAAGCGAGC ATGAGATCAT CAGATGTCTG
    AAAGCTTTTA TGAATAACAA TTTTGGAATT AAGGCAATGC TGGGCACAGA AGATGGCATT
    TTGCTGTTAG CGAGAGCAGT AGATCGATCT GTCCCTGCAA TGATGATTGA TTCCTTGAAG
    CTGCTTTCTG CCCTGTGCAT CCTGCCACAA CCAGAGGACA TGCATGAGCG TGTTCTAGAA
    GCCCTTACGG AGAGAGCAGA GATGGAGGAA ATGGAACGAT TTAAACCTCT TCTGGATGGG
    TTAAACATTG CAAGTCCTGT GGCCTTGAAG GTGAGCTGTA TGCAGCTAAT CAATGCAGTG
    ATCAGCCAAG GAGAAGAGCT TGATTACAGA GTTCACATCC GAAGTGAATT GATGCGCTCG
    GGACTGGCCA AACTCCTAAA GGAACTACGT AATACAGAAA ATGAAGACCT GAAGGTACAA
    CTTGGTATTT TTGATGAGCA TTCTGAACTT GATTCTGATG ACTTGCGAGG ACGTTTGGAC
    GACATCCGCA TTGAAATGGA TGACTTCAGT GACGTCTTCC AAGTTCTTCT GAACACTGTC
    AAGGACTCCT CTGCAGAACC ATTATTTCTT TCCATCCTGC AGCATCTTCT TCTAATTAGA
    AATGATTATG ATGCCAGGCC GCAGTACTAT AAACTGATAG ATGAAGTTAT ATCACAGATA
    ATCCTTCAAA AAAATGGGGC TGATCCAGAT TTCAAATGCA AGCATGTAAA CATAGAAATC
    GAAAATCTGA TAGATCATTT TATCGACAAA AAGAAAGTAG AAACAAGCGA GTCAAAAGCA
    CGGGATCTGG TGAAAAAGTT GGACCTAGAG CTTACCGCAC GCCACGAACT CCAGGTGGAA
    ATGAAAGCCA AAGAGAGCGA CTTTGAGCTG AAAATAACAG AGCTGCAGGG GGAGAAGGAA
    TCTTTAGATG CAGAGAAGCA AGAAATCACT GCTGACAAGC AAAAAATGGA GACAGAACTC
    TCCCAACTTG TAGAGCAGGT TGAAAAATTG TCAAAGGAAC TTGAAGCTAC CAAAAAAGAA
    ATGGCATCAA TTTCCACATC TGCTGTTTTA ATCCCTGTAC CTTCTGCCTC TGGAAATGTG
    CCACCTCCAC CACCCCCTCC TCCTCCACTT CCAGGTGCAA CTGGTATTCC TCCACCCCCC
    CCACTTCCAG GTGGCTCTGG TATTCCTCCA CCTCCCCCAC TTCCAGGTGG CTCTGGTATT
    CCTCCACCAC CCCCACTTCC AGGTGGCTCT GGTATTTCTC CACCACTTCC GGGTGGCTTT
    GGTATTCCTC CACCTCCCCC ACTTCCGGGT GGCTTTGGTA TTCCTCCACC TCCACCACTT
    CCCGGTGGCT CTGGTATTCC TCCACCTCCC CCACTTCCGG GTGGCTCTGG TATTCCTCCA
    CCTCCCCCAC TTCCGGGTGG CTCTGGTATT CCTCCACCTC CCCCACTTCC GGGTGGCTCT
    GGTATTCCTC CCCCACCACC ACTTCCGGGT GGCTCTGGTA TTCCTCCCCC ACCACCATTT
    CCGGGTGGCC CTGGAATTCC TCCCCCACCG CCATTTCCAG GAGGCTTTGG AATTCCTCCT
    CCTCCTCCTC CATTTCCAGG TGGCCCTGGA ATTCCTCCTC CTCCTCCATT CCCTGGTGGC
    CCTGGGGTTC CACCTCCACC TCCAGCATTT GGAGGTGGTG TTCCTCCACC ACCAGGACTT
    GGAGGCTGGG GGGCACCACC ACCCTTACCA TTTGGCTTGG CTCCTAAGAA GAATTATAAG
    CCAGAAGTGC AACTGAAGCG ACCCAACTGG TCAAAGATCA CTCCTGCTGA GCTTTCAGAG
    AAATCGTTTT GGACTCAGAC AAAAGAAGAT CGTTTTGAAA AAGAAGAGCT GTTTGCAAAA
    ATAACCCTCA CTTTTTCTTC ACAGATCAAG TCTTCTAAAG CAAAAAAAGC AGAAGATGGT
    GATGACAAAA AAGCAGCCCA GAAGAAAAAG ACAAAGGAGC TCAAAGTCTT GGATTCCAAG
    ACAGCCCAAA ACCTATCAAT CTTCCTTGGG TCGTTCCGCA TGCCATACGA AGAGATCAAA
    AATGTGATCC TACAGGTCAA TGAGGAAGTG CTGACTGAAT CTATGGTTTC AAATCTGATC
    AAGCAGTTAC CAGAACCAGA TCAGTTACAG ATGTTAACAG AGTTCAAGAA TGAGTACAAG
    GATTTGGCAG AACCAGAGCA GTTTGGAGTA GTGATGTGCA GTGTCCCCCG TTTGCGGCCA
    CGACTAAATG CCATTCTCTT TAAACTTCTT TTTAATGAGC AAATTGAAAA TATCAAGCCA
    GACATTGTGT CAGTTACAGC TGCTTGCGAA GAAGTGCAAA AGTGCCAAAG CTTTGCTAGT
    CTCCTGGAAA TCACACTGCT GGTTGGGAAC TTCATGAACG CAGGATCAAG GAACGCTGAT
    GCTTATGGCT TTGACATTAG CTTTCTCTGC AAGTTGAAAG ATACCAAATC AGCGGACCAG
    AAGACCACAC TGCTGCATTT CCTTGTTGAG ACTTGTGAGA ATGACTACCC TGATGTACTG
    AAGTTTCCAG ATGAGATGGT TCATGTAGAA AAGGCCAGTA GAGTGTCAGC TGAAACATTG
    CAAAAGAACC TGGATCAAAT GAAAAAGCAG ATTGCAGACC TTCAAAGGGA CATTGATAGC
    TTCCCACCAA CAGAAGATGA GAAGGATAAG TTTGTAGAAA AAATGACAAT ATTTGTCAAA
    GAAGCTCAAG AACAGTATAC TAAGCTTTGC ATGATGCACG AAAACATGCT GTCATTGTTC
    CAAGATTTGG GGAAATACTT TGTGTTTGAC CCCAAGAAAA CATCCATTGA AGAGTTCTTC
    GCAGACCTGC AGAATTTCCG GAACATGTTC TTGCACGCTC TGAAAGAGAA CCAGAAGAGA
    CGGGAAGCAG AAGAAAAGAT GAAAAGAGCC AAACTTGCCA AAGAGAAGGC AGAGAAGGAA
    CGGTTAGAGA AGCAGCAGAA GAAAGAACAA CTGATTGATA TGAATGCAGA GGGTGACGAA
    ACTGGCGTAA TGGACAGCCT GCTTGAAGCT TTGAATTCGG GAGCTGCATT CAGAAGGAAA
    AGGGGTCCTC GGCAAGACTC TCTCCTGTCC CTCAGTGAGG AGGAGGAGCA GGCAGCGGAC
    TTGGTAACTG TTGGGTGCCT TCGCGTGTCA TGTATTTGTG CTAACAACCC CCTTTTAACT
    GCACACATCC GGCATTTCCC TGTTCATTTT ATATTCATTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815149.1
    CDS208..4089
    Translation

    Target ORF information:

    RefSeq Version XM_012959695.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959695.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
    AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA
    TTTACCTTTA AGCGGCTGAT GGTAGATGAG CTTGAACGAT TTACCAGTAT ACGGATGAAG
    AAAGAAAAGG AAAAGCCTTA TCCATCACAC AGAAACTCCA CCAACTTCCC AGATAACTCA
    AACCCCCTCC AAACCTTGCA GGAACTCGAA GATGATGTTG TGCTTCAGCT GTTTGAAAAA
    ATGCTGGATG ATATGAACCT CAATCCTGAG AAGCAGCAAC CGCTACGAGA AAAGGATATA
    ATGATAAAAA GAGAGATGGT TTCCCAATAT CTTCACACAT CCAAAGCTGG CATGAGCAAA
    AAGGAGAGTT CTCGTTCTGC ATTAATGTAC ATTCAAGACT TAAAATCCGG ACTAGGAGAC
    AATTCCCTCC TTTTGTGCTT AGAGTCACTA AGAGTATCTT TAAATAACAA TCCTGTCAGC
    TGGGTGCAGA ATTTTGGTGC AGAGGGTCTT TCGTGCCTTT TAGAAATTCT CAAAAGAATA
    CAGGATGAGC AAGCATCCTT GCGGGATGTT AGAAGCGAGC ATGAGATCAT CAGATGTCTG
    AAAGCTTTTA TGAATAACAA TTTTGGAATT AAGGCAATGC TGGGCACAGA AGATGGCATT
    TTGCTGTTAG CGAGAGCAGT AGATCGATCT GTCCCTGCAA TGATGATTGA TTCCTTGAAG
    CTGCTTTCTG CCCTGTGCAT CCTGCCACAA CCAGAGGACA TGCATGAGCG TGTTCTAGAA
    GCCCTTACGG AGAGAGCAGA GATGGAGGAA ATGGAACGAT TTAAACCTCT TCTGGATGGG
    TTAAACATTG CAAGTCCTGT GGCCTTGAAG GTGAGCTGTA TGCAGCTAAT CAATGCAGTG
    ATCAGCCAAG GAGAAGAGCT TGATTACAGA GTTCACATCC GAAGTGAATT GATGCGCTCG
    GGACTGGCCA AACTCCTAAA GGAACTACGT AATACAGAAA ATGAAGACCT GAAGGTACAA
    CTTGGTATTT TTGATGAGCA TTCTGAACTT GATTCTGATG ACTTGCGAGG ACGTTTGGAC
    GACATCCGCA TTGAAATGGA TGACTTCAGT GACGTCTTCC AAGTTCTTCT GAACACTGTC
    AAGGACTCCT CTGCAGAACC ATTATTTCTT TCCATCCTGC AGCATCTTCT TCTAATTAGA
    AATGATTATG ATGCCAGGCC GCAGTACTAT AAACTGATAG ATGAAGTTAT ATCACAGATA
    ATCCTTCAAA AAAATGGGGC TGATCCAGAT TTCAAATGCA AGCATGTAAA CATAGAAATC
    GAAAATCTGA TAGATCATTT TATCGACAAA AAGAAAGTAG AAACAAGCGA GTCAAAAGCA
    CGGGATCTGG TGAAAAAGTT GGACCTAGAG CTTACCGCAC GCCACGAACT CCAGGTGGAA
    ATGAAAGCCA AAGAGAGCGA CTTTGAGCTG AAAATAACAG AGCTGCAGGG GGAGAAGGAA
    TCTTTAGATG CAGAGAAGCA AGAAATCACT GCTGACAAGC AAAAAATGGA GACAGAACTC
    TCCCAACTTG TAGAGCAGGT TGAAAAATTG TCAAAGGAAC TTGAAGCTAC CAAAAAAGAA
    ATGGCATCAA TTTCCACATC TGCTGTTTTA ATCCCTGTAC CTTCTGCCTC TGGAAATGTG
    CCACCTCCAC CACCCCCTCC TCCTCCACTT CCAGGTGCAA CTGGTATTCC TCCACCCCCC
    CCACTTCCAG GTGGCTCTGG TATTCCTCCA CCTCCCCCAC TTCCAGGTGG CTCTGGTATT
    CCTCCACCAC CCCCACTTCC AGGTGGCTCT GGTATTTCTC CACCACTTCC GGGTGGCTTT
    GGTATTCCTC CACCTCCCCC ACTTCCGGGT GGCTTTGGTA TTCCTCCACC TCCACCACTT
    CCCGGTGGCT CTGGTATTCC TCCACCTCCC CCACTTCCGG GTGGCTCTGG TATTCCTCCA
    CCTCCCCCAC TTCCGGGTGG CTCTGGTATT CCTCCACCTC CCCCACTTCC GGGTGGCTCT
    GGTATTCCTC CCCCACCACC ACTTCCGGGT GGCTCTGGTA TTCCTCCCCC ACCACCATTT
    CCGGGTGGCC CTGGAATTCC TCCCCCACCG CCATTTCCAG GAGGCTTTGG AATTCCTCCT
    CCTCCTCCTC CATTTCCAGG TGGCCCTGGA ATTCCTCCTC CTCCTCCATT CCCTGGTGGC
    CCTGGGGTTC CACCTCCACC TCCAGCATTT GGAGGTGGTG TTCCTCCACC ACCAGGACTT
    GGAGGCTGGG GGGCACCACC ACCCTTACCA TTTGGCTTGG CTCCTAAGAA GAATTATAAG
    CCAGAAGTGC AACTGAAGCG ACCCAACTGG TCAAAGATCA CTCCTGCTGA GCTTTCAGAG
    AAATCGTTTT GGACTCAGAC AAAAGAAGAT CGTTTTGAAA AAGAAGAGCT GTTTGCAAAA
    ATAACCCTCA CTTTTTCTTC ACAGATCAAG TCTTCTAAAG CAAAAAAAGC AGAAGATGGT
    GATGACAAAA AAGCAGCCCA GAAGAAAAAG ACAAAGGAGC TCAAAGTCTT GGATTCCAAG
    ACAGCCCAAA ACCTATCAAT CTTCCTTGGG TCGTTCCGCA TGCCATACGA AGAGATCAAA
    AATGTGATCC TACAGGTCAA TGAGGAAGTG CTGACTGAAT CTATGGTTTC AAATCTGATC
    AAGCAGTTAC CAGAACCAGA TCAGTTACAG ATGTTAACAG AGTTCAAGAA TGAGTACAAG
    GATTTGGCAG AACCAGAGCA GTTTGGAGTA GTGATGTGCA GTGTCCCCCG TTTGCGGCCA
    CGACTAAATG CCATTCTCTT TAAACTTCTT TTTAATGAGC AAATTGAAAA TATCAAGCCA
    GACATTGTGT CAGTTACAGC TGCTTGCGAA GAAGTGCAAA AGTGCCAAAG CTTTGCTAGT
    CTCCTGGAAA TCACACTGCT GGTTGGGAAC TTCATGAACG CAGGATCAAG GAACGCTGAT
    GCTTATGGCT TTGACATTAG CTTTCTCTGC AAGTTGAAAG ATACCAAATC AGCGGACCAG
    AAGACCACAC TGCTGCATTT CCTTGTTGAG ACTTGTGAGA ATGACTACCC TGATGTACTG
    AAGTTTCCAG ATGAGATGGT TCATGTAGAA AAGGCCAGTA GAGTGTCAGC TGAAACATTG
    CAAAAGAACC TGGATCAAAT GAAAAAGCAG ATTGCAGACC TTCAAAGGGA CATTGATAGC
    TTCCCACCAA CAGAAGATGA GAAGGATAAG TTTGTAGAAA AAATGACAAT ATTTGTCAAA
    GAAGCTCAAG AACAGTATAC TAAGCTTTGC ATGATGCACG AAAACATGCT GTCATTGTTC
    CAAGATTTGG GGAAATACTT TGTGTTTGAC CCCAAGAAAA CATCCATTGA AGAGTTCTTC
    GCAGACCTGC AGAATTTCCG GAACATGTTC TTGCACGCTC TGAAAGAGAA CCAGAAGAGA
    CGGGAAGCAG AAGAAAAGAT GAAAAGAGCC AAACTTGCCA AAGAGAAGGC AGAGAAGGAA
    CGGTTAGAGA AGCAGCAGAA GAAAGAACAA CTGATTGATA TGAATGCAGA GGGTGACGAA
    ACTGGCGTAA TGGACAGCCT GCTTGAAGCT TTGAATTCGG GAGCTGCATT CAGAAGGAAA
    AGGGGTCCTC GGCAAGACTC TCTCCTGTCC CTCAGTGAGG AGGAGGAGCA GGCAGCGGAC
    TTGGTAACTG TTGGGTGCCT TCGCGTGTCA TGTATTTGTG CTAACAACCC CCTTTTAACT
    GCACACATCC GGCATTTCCC TGTTCATTTT ATATTCATTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.