fn1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following fn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa28344 XM_012970493.3
Latest version!
Xenopus tropicalis fibronectin 1 (fn1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa28345 XM_012970494.3
Latest version!
Xenopus tropicalis fibronectin 1 (fn1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa28346 XM_012970495.3
Latest version!
Xenopus tropicalis fibronectin 1 (fn1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa28347 XM_012970496.3
Latest version!
Xenopus tropicalis fibronectin 1 (fn1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa46596 XM_018097141.1
Latest version!
Xenopus tropicalis fibronectin 1 (fn1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OXa25257 NM_001030524.1
Latest version!
Xenopus tropicalis fibronectin 1 (fn1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
View Old Accession Versions >>
OXa28347 XM_012970496.2 Xenopus tropicalis fibronectin 1 (fn1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OXa28344 XM_012970493.2 Xenopus tropicalis fibronectin 1 (fn1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OXa28345 XM_012970494.2 Xenopus tropicalis fibronectin 1 (fn1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OXa28346 XM_012970495.2 Xenopus tropicalis fibronectin 1 (fn1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa28344
    Clone ID Related Accession (Same CDS sequence) XM_012970493.3 , XM_012970493.2
    Accession Version XM_012970493.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product fibronectin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970493.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCGCTGGG GGGCCCTGAC CGGGCTGCTC CTAGCCCTGT GCCTGTGTGC AGTGGTACGT 
    TCCGCCCCAT CAAGCAAGAA GCGCAGGCAG GCGCAGCAGC AGCAAGTAGT GCAACCCCAT
    GGATCACCGG ACAGCCAGCA GAAAGGATGT TATGACAATG GCAAGTACTA CCAAATCAAC
    CAGCAATGGG AGCGCACCTA CCTAGGGAAC ACACTGGTGT GTACTTGTTA TGGCGGAGGC
    AGAGGTTTCA ACTGCGAAAG CAAACCAGAG TCTGAAGAGA CCTGCTTTGA CAAATACACT
    GGTGTTACTT ACCGGGTCGG TGAAACATAT GAGCGACCAA AGGATAACAT GATCTGGGAC
    TGTACGTGTA TTGGAGCTGG AAGGGGACGC ATTAGCTGTA CAATTGCAAA TCGTTGCCAT
    GAAGGAGGTC AGTCTTATAA GATTGGGGAC ACCTGGCGCA GGCCACATGA AACTGGGGGG
    TATATGCTAG AATGTGTCTG CCTTGGAAAT GGTAAAGGAG AATGGACCTG CAAGCCCGTT
    GCTGAGAGAT GCTATGATAA CACAGCTGGG ATATCCTATG TAGTTGGTCA GACCTGGGAA
    AAGCCTTATC AGGGCTGGAT GATGGTGGAC TGTACATGCT TGGGAGAAGG CAGTGGACGA
    ATCACATGTT CTTCCAAAAA CAGATGCAAT GACCAGGACA CCAGAACTTC TTATAGAATT
    GGAAATTCAT GGAGTAAGAC TGACACCAGA GGAAATCTCT TGCAATGTAT CTGTACTGGC
    AATGGGCGAG GAGAGTGGAA ATGTGATAGA CATTCCTCTG CACAAGCAAC TGGTACTGGC
    TCAAACCCCA TCACAAACAT TCAAACTACC CTCTTCCAGC CTGATTCAGA GCTGGAGCCC
    TATGGTCACT GTGTGACTGA CAATGGGGTC TTGTATTCTT TGGGTATGAG ATGGCTGAAG
    GCACAGGGCA GCAAGCAGAT GCTCTGTACC TGCCTTGGTA ATGGTGTGAG CTGTGAAGAA
    ACTGTTGAAA CCATCACATT TGGTGGTAAT GCCAATGGTG AGCCATGTGC CATTCCTTTC
    ACTCATGATG GCAAGACCTA TTATTCCTGC ACAGGAGAGG GTCGTCAGGA TGGAAAATTG
    TGGTGTGCAA CAACCTCCAA CTATGACACT GACAAGAAGT ATTCATTCTG CAATGAGCAG
    CGTGCACTTG TACAGACACG TGGTGGTAAC TCCAATGGAG CTCTTTGCAA CTTTCCATTT
    TTGTACAACA ACCGCAACTA TACAGACTGC ACCTCTGAAG GTCGGAGGGA CAGTATGAAG
    TGGTGCGGGA CTACTGCCAA CTATGATGCC GATCAAAAAT TTGGATTCTG TCCCATGGCA
    GCTCATGAGG AAATATGCAC TACTAATGAG GGAGTCATGT ATAGAGTTGG TGATCAGTGG
    GACAAGCAGC ATGATCAAGG CCACATGATG CGATGTACTT GTGTTGGAAA TGGTCGTGGG
    GAATGGACTT GTGTGGCTTA TTCTCAGCTT AAAGACCAGT GCATTGTGGA TGGGATAACT
    TATGATGTGA ACAGTTCTTT TACGAAACGC CATGAGGAAG GACACATGAT GAACTGTACC
    TGCTATGGGC AGGGTCGTGG CAGATGGAAG TGCGATGCTA TAGACCAATG CCAGGATACT
    GAAACCCGAC AGTTCTATCA GATTGGAGAT TCCTGGGAAA AGCATTTGCA GGGAGTACGG
    TACCAGTGTT ACTGCTATGG CAAAGGAATT GGTGAATGGC ATTGCCAGCC TCTATCAACA
    TCCCCAGCTG GGACTGGACC CGTTCAGGTT ATTATCACAG AGAGTTCCAA TTTCCCTAAT
    TCTCACCCTA TACAGTGGAA TGCCCCACAG CCATCACACA TTAAGAATTA CATCCTGCGC
    TGGAAGCCTA AACTGAAGAC TGGCCCATGG AAACAAGCCA CTATTCCAGG ACACCTGAAC
    TCTTATACCA TCTCTGGCTT GAAACCTGGA ATCCTGTATG AGGGGCAATT GATCAGCATT
    CTGCAGTATG GAAACAGGGA GGTCACAACC TTTGACTTTA CCACCACTTC TACCATTCAC
    CGCTCCAGTC AAACTGAGAC AGGAGAAACT ACCCCACTTC CACCATTGGT GGCCACATCT
    GAGTCTGTAA CAGAAATTAC TGCTAGCAGT TTTCTGGTAT CCTGGGTCTC TGCATCTGAC
    ACAGTGTCTG GCTTCCGTGT TGAATATGAG CTGAGTGAGG ATGGAGATGA GAAACGCTAC
    CTAGAACTTC CAAACACTGC CACTTCAGTC AGTATCCCAG ACCTCCTCCC AGGCCGTAGA
    TACAATGTGA ATGTGTATCA GATAACTGAA GAGGGTGAAA AGAGCCTCAT CCTATCAACT
    ACTCAGACAA CTGCACCTGA TGCACCCCCC GACCATAATG TGGAAAATGT TGATGACACC
    TCCATCCTGA TCAAATGGAC CAAACCTCAA GCTCCCGTCA CAGGATACAG GGTTGTATAC
    AGTCCATCTG TAGAAGGCAG CAGCACAGAG TTAAACCTCC CAAGTACTGC AACTTCAGTG
    AGCCTGACTG AGCTGCTCCC AGGAATTGAA TACAATATTA CAATCTATGC AGTGGAAGAC
    AGTTTAGAAA GTGTTCCCAT CTTTATTCAG CAAGGGACAA CTGGAACTCC ACAGACAGTT
    ATAGTACCAT CTCCCACTGA CTTACAATTA GTGGAAGTTA CCGATATAAA AATTACCATC
    ATGTGGAACC CCCCACAAAG TGAGGTTTCT GGATACCGAG TTGTTGTGAA ACCTGTAAGT
    CCCTCTGACC GTGATGTGCA GAATCTCCCC GTGAGCAGGA ACACTTTTGC CGAGGTGGTT
    AACTTGCACC CTGGCAGGAC CTATACCTTT GAGGTGTATG CAGTTAATCG TGGACAAGAG
    AGTGAACCCC TGGCTGGAGA CTTCACTACC AAACTCGATG CTCCATCAGA CTTGCAGTTT
    ACAGATGTAA CTGACTCTAC AGTTGTTATT ATCTGGAGAC CACCCCAAGC CAAAATTGGT
    CGTTACCTGC TGTCTGTAGG TCAAACCCGG GGAGGCCAGC CGAGCCAATT TCACATTAAC
    CCCTCTGCCA CAAACCACAA GCTGGAGAGT CTCTCCCCAG GCACAGAGTA CACCATTTCA
    CTTGTTGCCC TCAAAGGCAA TCAGCAAAGT GCTAGCACTA CTGGTGTCTT TTCAACATTG
    GAACCTGTGG GATCAATTCC ACCTTACAAC ACTGAAGTCA CAGAGACTAC CATAGTGGTA
    ACTTGGACCC CTGTGCCCAG AATCGGGTTT AAGCTGGATG TTCGACCCAG CCAAGGTGGG
    GAGGCACCTC GGGAAGTCAT ATCCGAGTCT GGCAGCATTG TGATCTCTGG CCTAACCCCT
    GGAGTCGAAT ATATGTACAG CATATCTGTT CTTAAGGATG GCGTTGAGAG GGACATTCCC
    ATCACCAAGA CTGTTGTCAC ACCTGTCGCT CCTCCAACCA ACTTGCGCCT CCAACCAAGC
    AGAGATTCTG CTACACTTAC TGTTTACTGG GACAGTAGCA TTTCACCAGG AATCACTGGA
    TACAGAATTA CCACTGCACC CACTCCAATG CAGGTTGGCA ATTCCCTGGA AGAGGAGGTT
    GGTCCAACTC AGACATACTG CCTCTTTGAG AACCTGAGCC CTGGTGTAGA GTACAATGTC
    AGCGTGTATG CAGTAAAAGG GGAAGAGGAG AGTTCACCTC TCTCCCAGAT CTTTCTTCAA
    GAGATACCCC AACTGACTGA CCTAAAGTAT GACGATGTAA CTGACACAAG CATTGACCTG
    AGGTGGACGC CCCTTAATTC CTCCACCATT ATCGGCTACC GAATCACGGT GGTTGCGGCG
    GGAGAATCCG TCCCTATCTA CGAGGAATTT GTGGGCCCCA CAGATGGGTA TTACAAAGTT
    TCAGGATTGG AACCCGGCAT TGACTATGAG ATCAGCGTGA TAACACTTAT TAGCGGCGGA
    GAGAGCGCCC CGACCACAAT CGTACAGCAC ACGGCTGTTC CACCTCCAAC TAATTTGCGC
    TTCACCAATG TTGGCCCTGA CACCATGCGC GTCACCTGGT CACCTCCAAC TTCTATCGAA
    CTATCAAGTT TCCTGGTACG ATATTCTCCC GTGAAGAAGC CAGATGATGT GACAGAACTC
    TCCCTCTCTC CATCTACCAA CATGGTGGTT TTATCAAACT TGCTTCCCTT CACCGAATAT
    TTAGTCAGCG TTCATTCTGT TTATGAGGAG CGAGAGAGTA GTGCCCTCAA TGGTGTAGCT
    AAGACTTACT TGGACTCTCC AACTGGCATT GACTTTTCTG AAATTACTCC CAATTCATTT
    ACTGTGCACT GGATTGCTCC ACGTGCCCCT ATCACTGGCT ACAGAATTCG GTACCAGTTA
    GAAAGTGGAG CTGGGCGACC CAAGGAGGAG CGGGTACCTC CATCCCGGAA CTCTATTACC
    CTTACCCACC TCATTCCAGG TTCAGAATAC ATTGTCAGTA TCATTGCATT CAATGGTCAA
    CAGGAGAGTT TGCCACTTTC TGGACAGCAA GCCACTGTAT CTGATGTCCC AACTGATTTG
    GAAGTGACCT CCTCCTCTCC TAACACACTC ACCATCTCTT GGGAAGCACC TTCAGTGAAT
    GTACGTTACT ACAGGATCAC CCATTCCCAG ACAGGAGGTC ATGGTCCAGA GAAGGAGTTT
    ACAGTTCCAG GAACATCCAA CACTGCAACC ATCAGAGGGT TAAATCCAGG AGTAAGCTAC
    ACCATCACTG TTTATGCAGT GACTGGGCGA GGGGATAGTC CTGCCTCTAG CAAACCACTT
    ACCATTGTTC ACATGACAGA TGTTGACCAA CCAACTGACA TGGCTGTCAC AGATATCCAG
    GATCACAGCA TTCATGTCAA ATGGTCTCCT CCTCCAGGCC CAGTCACTGG TTACAGGGTC
    ACTAGTGTCC CAAAAAGTGG CCAAGGAGAA ACCTTTTCCC AGGTCGTTTC TCCAGATCAA
    ACTGAAGTGA CGATTGTGGG CCTGCAACCA GCTGTGGAGT ATGTGGTGAG CATATACTCA
    CAAGGTGAAA ATGGCGAGAG CGAGCCTCTG GTTGAAACTG CAGTTACAAC AATTCCAGCA
    CCAACAAATC TTCAGTTCTC TCAAGTGACA CCAAGTGGCT TCTCTCTTAG CTGGCATGCA
    CCCACTATCC ATCTCAGTGG GTACCTTGTA CGTGTCAACC CTAAAGAGAA AACTGGGCCT
    ATGAAGGAAG TTAGACTTTC ACCTGGTGTG ACTGCTACAA CTGTAACTGG TCTGATGGTG
    GCCACAAAAT ATGAAGTTAA TGTGTATGCT CTTAAGGATA CCCTGACAAG TCAGCCACTG
    CAGGGTTTAA TCTCTACACT TGATAATGTA AGTCCACCTC GAAGACCTCG TGTTCAAGAT
    GTGACCGAGA CCACAGTTAC ACTGTCCTGG CGCACTAAAA CAGAGACCAT CACTGGCTTC
    CAGATTGATG CTATACCTGC CGGAGGTCAG AATCCCATCA GGAGAACAGT TGATGCAGAT
    CTCCGTTCTT TTACTATCAC AGGTCTGCAG CCTGGCACAG ACTACAAGAT CTATCTGTAC
    ACCCTAAATG ACAATGCTCG TAGCTCTCCT GTGATTGTTG ATGTCACCAC AGCTGTGGAC
    TCTCCATCCA ACCTGCGTTT CCTGACCACA ACTAGCAACT CCCTGCTGTT CACTTGGCAG
    CCACCTCGTG CTCGCATCAC TGGCTATATC ATTCGTTATG AGAAAGCTGG AGGGCTACTG
    AAAGAGCACC TCCCACGCCT CCAAGCAGGA ACCACAGAGT CTACCATTAC CAACCTGGAG
    CCAGGCACAG AATATATTGT CTATATTATT GCTGTGAGAA ACAATCAGAA GAGTGAGCCT
    CTTGTGGGAC GTAAGAGAAC AGACGAACTG CCTCGCCTGG TTACACTTCC ACACCCGGGC
    AAAGGCCCTG AAATCCTTGA TGTTCCCACT GATGAAGAGA ACACACCCCA CATCACACAA
    ATCAAGTTGG ACAATGGTAA TGGTATCCAA CTGCCAGGCT CTAATGGACA GCAGCCCAGT
    TCTGGCAATG AAGGGCAGCT TATAGAAGAA CATGGTTTCA GAAGTCCCCT TGCACCCACT
    ACTGCTGTGC CAGTGAGGCC AGGGAAATTC ACCCCTGGGC ACTACCCACA GGAGAGGGTT
    GATATTGAGC TAGACACAGA GTTCCCTGTG CAGCGTGGAG ATCTTGATGG TCCTTACCCT
    CATGGCTTAG GACCACAGCT AAATGATTCT GGCGTCCAAG AAGTGGCATC CCATACTACC
    ATTTCATGGA GACCTGAGCT GGAAACCACT GAATATATCA TCTCTTGCCA TCCTATTGAA
    CATGATGAGG CCCCATTGCA GTTTAGGGTC CCTGGAACCT CCTCTAGTGC CACATTAAAT
    GGTCTGACTC AAGGAGCCAC TTATAACATC ATTGTGGAAG CGCAGAAGGG GTCTGATAAA
    CATAAGGTCT TCGAGAAGCG TGTAACTGTT GGCAGTCCTG GTTCCCCTGA GGGGGTTTTA
    CAGCCAGCTG AGGACACCTG TTATGACACA TTCAATGGAG CACACTATTC TGTGGGACAG
    GAATGGGAAC GCATGTCAGA ATCTGGATTC AGGCTCTGGT GCAAGTGCCT GGGCTATGGA
    AGTGGACACT TCAGATGTGA CTCCTCCAAA TGGTGCCATG ATAATGGTGT CAATCACAGG
    GTCGGGGAGA AATGGGACCG ACGAGGAGAG AATGGTCAGA TGATGAGTTG TACTTGTCTT
    GGCAATGGAA AAGGAGAATT TAAGTGTGAA CCTCATGAAG CCACGTGCTA TGATGAAGGG
    AAGATGTACA ATGTTGGAGA GCAGTGGCAG AAGGAATATT TAGGGGCAAT CTGTTCATGC
    ACATGCTATG GAGGACAGCA AGGGTGGAGA TGTGACAATT GCCGCAGACC AGGTGCTGTT
    CTTCCCGATG GCACTGCTGG GCATACAGTC TCTCAGTTTG CCCAGAGATA CCAACAGAAC
    TATAATTTGA ACTGCCCCAT TGAGTGCTAC CTTCCCCTCC AAGCAGATAT ACAGCATTCG
    GAACAGACCC AGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825947.1
    CDS222..7379
    Translation

    Target ORF information:

    RefSeq Version XM_012970493.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis fibronectin 1 (fn1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970493.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCGCTGGG GGGCCCTGAC CGGGCTGCTC CTAGCCCTGT GCCTGTGTGC AGTGGTACGT 
    TCCGCCCCAT CAAGCAAGAA GCGCAGGCAG GCGCAGCAGC AGCAAGTAGT GCAACCCCAT
    GGATCACCGG ACAGCCAGCA GAAAGGATGT TATGACAATG GCAAGTACTA CCAAATCAAC
    CAGCAATGGG AGCGCACCTA CCTAGGGAAC ACACTGGTGT GTACTTGTTA TGGCGGAGGC
    AGAGGTTTCA ACTGCGAAAG CAAACCAGAG TCTGAAGAGA CCTGCTTTGA CAAATACACT
    GGTGTTACTT ACCGGGTCGG TGAAACATAT GAGCGACCAA AGGATAACAT GATCTGGGAC
    TGTACGTGTA TTGGAGCTGG AAGGGGACGC ATTAGCTGTA CAATTGCAAA TCGTTGCCAT
    GAAGGAGGTC AGTCTTATAA GATTGGGGAC ACCTGGCGCA GGCCACATGA AACTGGGGGG
    TATATGCTAG AATGTGTCTG CCTTGGAAAT GGTAAAGGAG AATGGACCTG CAAGCCCGTT
    GCTGAGAGAT GCTATGATAA CACAGCTGGG ATATCCTATG TAGTTGGTCA GACCTGGGAA
    AAGCCTTATC AGGGCTGGAT GATGGTGGAC TGTACATGCT TGGGAGAAGG CAGTGGACGA
    ATCACATGTT CTTCCAAAAA CAGATGCAAT GACCAGGACA CCAGAACTTC TTATAGAATT
    GGAAATTCAT GGAGTAAGAC TGACACCAGA GGAAATCTCT TGCAATGTAT CTGTACTGGC
    AATGGGCGAG GAGAGTGGAA ATGTGATAGA CATTCCTCTG CACAAGCAAC TGGTACTGGC
    TCAAACCCCA TCACAAACAT TCAAACTACC CTCTTCCAGC CTGATTCAGA GCTGGAGCCC
    TATGGTCACT GTGTGACTGA CAATGGGGTC TTGTATTCTT TGGGTATGAG ATGGCTGAAG
    GCACAGGGCA GCAAGCAGAT GCTCTGTACC TGCCTTGGTA ATGGTGTGAG CTGTGAAGAA
    ACTGTTGAAA CCATCACATT TGGTGGTAAT GCCAATGGTG AGCCATGTGC CATTCCTTTC
    ACTCATGATG GCAAGACCTA TTATTCCTGC ACAGGAGAGG GTCGTCAGGA TGGAAAATTG
    TGGTGTGCAA CAACCTCCAA CTATGACACT GACAAGAAGT ATTCATTCTG CAATGAGCAG
    CGTGCACTTG TACAGACACG TGGTGGTAAC TCCAATGGAG CTCTTTGCAA CTTTCCATTT
    TTGTACAACA ACCGCAACTA TACAGACTGC ACCTCTGAAG GTCGGAGGGA CAGTATGAAG
    TGGTGCGGGA CTACTGCCAA CTATGATGCC GATCAAAAAT TTGGATTCTG TCCCATGGCA
    GCTCATGAGG AAATATGCAC TACTAATGAG GGAGTCATGT ATAGAGTTGG TGATCAGTGG
    GACAAGCAGC ATGATCAAGG CCACATGATG CGATGTACTT GTGTTGGAAA TGGTCGTGGG
    GAATGGACTT GTGTGGCTTA TTCTCAGCTT AAAGACCAGT GCATTGTGGA TGGGATAACT
    TATGATGTGA ACAGTTCTTT TACGAAACGC CATGAGGAAG GACACATGAT GAACTGTACC
    TGCTATGGGC AGGGTCGTGG CAGATGGAAG TGCGATGCTA TAGACCAATG CCAGGATACT
    GAAACCCGAC AGTTCTATCA GATTGGAGAT TCCTGGGAAA AGCATTTGCA GGGAGTACGG
    TACCAGTGTT ACTGCTATGG CAAAGGAATT GGTGAATGGC ATTGCCAGCC TCTATCAACA
    TCCCCAGCTG GGACTGGACC CGTTCAGGTT ATTATCACAG AGAGTTCCAA TTTCCCTAAT
    TCTCACCCTA TACAGTGGAA TGCCCCACAG CCATCACACA TTAAGAATTA CATCCTGCGC
    TGGAAGCCTA AACTGAAGAC TGGCCCATGG AAACAAGCCA CTATTCCAGG ACACCTGAAC
    TCTTATACCA TCTCTGGCTT GAAACCTGGA ATCCTGTATG AGGGGCAATT GATCAGCATT
    CTGCAGTATG GAAACAGGGA GGTCACAACC TTTGACTTTA CCACCACTTC TACCATTCAC
    CGCTCCAGTC AAACTGAGAC AGGAGAAACT ACCCCACTTC CACCATTGGT GGCCACATCT
    GAGTCTGTAA CAGAAATTAC TGCTAGCAGT TTTCTGGTAT CCTGGGTCTC TGCATCTGAC
    ACAGTGTCTG GCTTCCGTGT TGAATATGAG CTGAGTGAGG ATGGAGATGA GAAACGCTAC
    CTAGAACTTC CAAACACTGC CACTTCAGTC AGTATCCCAG ACCTCCTCCC AGGCCGTAGA
    TACAATGTGA ATGTGTATCA GATAACTGAA GAGGGTGAAA AGAGCCTCAT CCTATCAACT
    ACTCAGACAA CTGCACCTGA TGCACCCCCC GACCATAATG TGGAAAATGT TGATGACACC
    TCCATCCTGA TCAAATGGAC CAAACCTCAA GCTCCCGTCA CAGGATACAG GGTTGTATAC
    AGTCCATCTG TAGAAGGCAG CAGCACAGAG TTAAACCTCC CAAGTACTGC AACTTCAGTG
    AGCCTGACTG AGCTGCTCCC AGGAATTGAA TACAATATTA CAATCTATGC AGTGGAAGAC
    AGTTTAGAAA GTGTTCCCAT CTTTATTCAG CAAGGGACAA CTGGAACTCC ACAGACAGTT
    ATAGTACCAT CTCCCACTGA CTTACAATTA GTGGAAGTTA CCGATATAAA AATTACCATC
    ATGTGGAACC CCCCACAAAG TGAGGTTTCT GGATACCGAG TTGTTGTGAA ACCTGTAAGT
    CCCTCTGACC GTGATGTGCA GAATCTCCCC GTGAGCAGGA ACACTTTTGC CGAGGTGGTT
    AACTTGCACC CTGGCAGGAC CTATACCTTT GAGGTGTATG CAGTTAATCG TGGACAAGAG
    AGTGAACCCC TGGCTGGAGA CTTCACTACC AAACTCGATG CTCCATCAGA CTTGCAGTTT
    ACAGATGTAA CTGACTCTAC AGTTGTTATT ATCTGGAGAC CACCCCAAGC CAAAATTGGT
    CGTTACCTGC TGTCTGTAGG TCAAACCCGG GGAGGCCAGC CGAGCCAATT TCACATTAAC
    CCCTCTGCCA CAAACCACAA GCTGGAGAGT CTCTCCCCAG GCACAGAGTA CACCATTTCA
    CTTGTTGCCC TCAAAGGCAA TCAGCAAAGT GCTAGCACTA CTGGTGTCTT TTCAACATTG
    GAACCTGTGG GATCAATTCC ACCTTACAAC ACTGAAGTCA CAGAGACTAC CATAGTGGTA
    ACTTGGACCC CTGTGCCCAG AATCGGGTTT AAGCTGGATG TTCGACCCAG CCAAGGTGGG
    GAGGCACCTC GGGAAGTCAT ATCCGAGTCT GGCAGCATTG TGATCTCTGG CCTAACCCCT
    GGAGTCGAAT ATATGTACAG CATATCTGTT CTTAAGGATG GCGTTGAGAG GGACATTCCC
    ATCACCAAGA CTGTTGTCAC ACCTGTCGCT CCTCCAACCA ACTTGCGCCT CCAACCAAGC
    AGAGATTCTG CTACACTTAC TGTTTACTGG GACAGTAGCA TTTCACCAGG AATCACTGGA
    TACAGAATTA CCACTGCACC CACTCCAATG CAGGTTGGCA ATTCCCTGGA AGAGGAGGTT
    GGTCCAACTC AGACATACTG CCTCTTTGAG AACCTGAGCC CTGGTGTAGA GTACAATGTC
    AGCGTGTATG CAGTAAAAGG GGAAGAGGAG AGTTCACCTC TCTCCCAGAT CTTTCTTCAA
    GAGATACCCC AACTGACTGA CCTAAAGTAT GACGATGTAA CTGACACAAG CATTGACCTG
    AGGTGGACGC CCCTTAATTC CTCCACCATT ATCGGCTACC GAATCACGGT GGTTGCGGCG
    GGAGAATCCG TCCCTATCTA CGAGGAATTT GTGGGCCCCA CAGATGGGTA TTACAAAGTT
    TCAGGATTGG AACCCGGCAT TGACTATGAG ATCAGCGTGA TAACACTTAT TAGCGGCGGA
    GAGAGCGCCC CGACCACAAT CGTACAGCAC ACGGCTGTTC CACCTCCAAC TAATTTGCGC
    TTCACCAATG TTGGCCCTGA CACCATGCGC GTCACCTGGT CACCTCCAAC TTCTATCGAA
    CTATCAAGTT TCCTGGTACG ATATTCTCCC GTGAAGAAGC CAGATGATGT GACAGAACTC
    TCCCTCTCTC CATCTACCAA CATGGTGGTT TTATCAAACT TGCTTCCCTT CACCGAATAT
    TTAGTCAGCG TTCATTCTGT TTATGAGGAG CGAGAGAGTA GTGCCCTCAA TGGTGTAGCT
    AAGACTTACT TGGACTCTCC AACTGGCATT GACTTTTCTG AAATTACTCC CAATTCATTT
    ACTGTGCACT GGATTGCTCC ACGTGCCCCT ATCACTGGCT ACAGAATTCG GTACCAGTTA
    GAAAGTGGAG CTGGGCGACC CAAGGAGGAG CGGGTACCTC CATCCCGGAA CTCTATTACC
    CTTACCCACC TCATTCCAGG TTCAGAATAC ATTGTCAGTA TCATTGCATT CAATGGTCAA
    CAGGAGAGTT TGCCACTTTC TGGACAGCAA GCCACTGTAT CTGATGTCCC AACTGATTTG
    GAAGTGACCT CCTCCTCTCC TAACACACTC ACCATCTCTT GGGAAGCACC TTCAGTGAAT
    GTACGTTACT ACAGGATCAC CCATTCCCAG ACAGGAGGTC ATGGTCCAGA GAAGGAGTTT
    ACAGTTCCAG GAACATCCAA CACTGCAACC ATCAGAGGGT TAAATCCAGG AGTAAGCTAC
    ACCATCACTG TTTATGCAGT GACTGGGCGA GGGGATAGTC CTGCCTCTAG CAAACCACTT
    ACCATTGTTC ACATGACAGA TGTTGACCAA CCAACTGACA TGGCTGTCAC AGATATCCAG
    GATCACAGCA TTCATGTCAA ATGGTCTCCT CCTCCAGGCC CAGTCACTGG TTACAGGGTC
    ACTAGTGTCC CAAAAAGTGG CCAAGGAGAA ACCTTTTCCC AGGTCGTTTC TCCAGATCAA
    ACTGAAGTGA CGATTGTGGG CCTGCAACCA GCTGTGGAGT ATGTGGTGAG CATATACTCA
    CAAGGTGAAA ATGGCGAGAG CGAGCCTCTG GTTGAAACTG CAGTTACAAC AATTCCAGCA
    CCAACAAATC TTCAGTTCTC TCAAGTGACA CCAAGTGGCT TCTCTCTTAG CTGGCATGCA
    CCCACTATCC ATCTCAGTGG GTACCTTGTA CGTGTCAACC CTAAAGAGAA AACTGGGCCT
    ATGAAGGAAG TTAGACTTTC ACCTGGTGTG ACTGCTACAA CTGTAACTGG TCTGATGGTG
    GCCACAAAAT ATGAAGTTAA TGTGTATGCT CTTAAGGATA CCCTGACAAG TCAGCCACTG
    CAGGGTTTAA TCTCTACACT TGATAATGTA AGTCCACCTC GAAGACCTCG TGTTCAAGAT
    GTGACCGAGA CCACAGTTAC ACTGTCCTGG CGCACTAAAA CAGAGACCAT CACTGGCTTC
    CAGATTGATG CTATACCTGC CGGAGGTCAG AATCCCATCA GGAGAACAGT TGATGCAGAT
    CTCCGTTCTT TTACTATCAC AGGTCTGCAG CCTGGCACAG ACTACAAGAT CTATCTGTAC
    ACCCTAAATG ACAATGCTCG TAGCTCTCCT GTGATTGTTG ATGTCACCAC AGCTGTGGAC
    TCTCCATCCA ACCTGCGTTT CCTGACCACA ACTAGCAACT CCCTGCTGTT CACTTGGCAG
    CCACCTCGTG CTCGCATCAC TGGCTATATC ATTCGTTATG AGAAAGCTGG AGGGCTACTG
    AAAGAGCACC TCCCACGCCT CCAAGCAGGA ACCACAGAGT CTACCATTAC CAACCTGGAG
    CCAGGCACAG AATATATTGT CTATATTATT GCTGTGAGAA ACAATCAGAA GAGTGAGCCT
    CTTGTGGGAC GTAAGAGAAC AGACGAACTG CCTCGCCTGG TTACACTTCC ACACCCGGGC
    AAAGGCCCTG AAATCCTTGA TGTTCCCACT GATGAAGAGA ACACACCCCA CATCACACAA
    ATCAAGTTGG ACAATGGTAA TGGTATCCAA CTGCCAGGCT CTAATGGACA GCAGCCCAGT
    TCTGGCAATG AAGGGCAGCT TATAGAAGAA CATGGTTTCA GAAGTCCCCT TGCACCCACT
    ACTGCTGTGC CAGTGAGGCC AGGGAAATTC ACCCCTGGGC ACTACCCACA GGAGAGGGTT
    GATATTGAGC TAGACACAGA GTTCCCTGTG CAGCGTGGAG ATCTTGATGG TCCTTACCCT
    CATGGCTTAG GACCACAGCT AAATGATTCT GGCGTCCAAG AAGTGGCATC CCATACTACC
    ATTTCATGGA GACCTGAGCT GGAAACCACT GAATATATCA TCTCTTGCCA TCCTATTGAA
    CATGATGAGG CCCCATTGCA GTTTAGGGTC CCTGGAACCT CCTCTAGTGC CACATTAAAT
    GGTCTGACTC AAGGAGCCAC TTATAACATC ATTGTGGAAG CGCAGAAGGG GTCTGATAAA
    CATAAGGTCT TCGAGAAGCG TGTAACTGTT GGCAGTCCTG GTTCCCCTGA GGGGGTTTTA
    CAGCCAGCTG AGGACACCTG TTATGACACA TTCAATGGAG CACACTATTC TGTGGGACAG
    GAATGGGAAC GCATGTCAGA ATCTGGATTC AGGCTCTGGT GCAAGTGCCT GGGCTATGGA
    AGTGGACACT TCAGATGTGA CTCCTCCAAA TGGTGCCATG ATAATGGTGT CAATCACAGG
    GTCGGGGAGA AATGGGACCG ACGAGGAGAG AATGGTCAGA TGATGAGTTG TACTTGTCTT
    GGCAATGGAA AAGGAGAATT TAAGTGTGAA CCTCATGAAG CCACGTGCTA TGATGAAGGG
    AAGATGTACA ATGTTGGAGA GCAGTGGCAG AAGGAATATT TAGGGGCAAT CTGTTCATGC
    ACATGCTATG GAGGACAGCA AGGGTGGAGA TGTGACAATT GCCGCAGACC AGGTGCTGTT
    CTTCCCGATG GCACTGCTGG GCATACAGTC TCTCAGTTTG CCCAGAGATA CCAACAGAAC
    TATAATTTGA ACTGCCCCAT TGAGTGCTAC CTTCCCCTCC AAGCAGATAT ACAGCATTCG
    GAACAGACCC AGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa28345
    Clone ID Related Accession (Same CDS sequence) XM_012970494.3 , XM_012970494.2
    Accession Version XM_012970494.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product fibronectin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970494.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCGCTGGG GGGCCCTGAC CGGGCTGCTC CTAGCCCTGT GCCTGTGTGC AGTGGTACGT 
    TCCGCCCCAT CAAGCAAGAA GCGCAGGCAG GCGCAGCAGC AGCAAGTAGT GCAACCCCAT
    GGATCACCGG ACAGCCAGCA GAAAGGATGT TATGACAATG GCAAGTACTA CCAAATCAAC
    CAGCAATGGG AGCGCACCTA CCTAGGGAAC ACACTGGTGT GTACTTGTTA TGGCGGAGGC
    AGAGGTTTCA ACTGCGAAAG CAAACCAGAG TCTGAAGAGA CCTGCTTTGA CAAATACACT
    GGTGTTACTT ACCGGGTCGG TGAAACATAT GAGCGACCAA AGGATAACAT GATCTGGGAC
    TGTACGTGTA TTGGAGCTGG AAGGGGACGC ATTAGCTGTA CAATTGCAAA TCGTTGCCAT
    GAAGGAGGTC AGTCTTATAA GATTGGGGAC ACCTGGCGCA GGCCACATGA AACTGGGGGG
    TATATGCTAG AATGTGTCTG CCTTGGAAAT GGTAAAGGAG AATGGACCTG CAAGCCCGTT
    GCTGAGAGAT GCTATGATAA CACAGCTGGG ATATCCTATG TAGTTGGTCA GACCTGGGAA
    AAGCCTTATC AGGGCTGGAT GATGGTGGAC TGTACATGCT TGGGAGAAGG CAGTGGACGA
    ATCACATGTT CTTCCAAAAA CAGATGCAAT GACCAGGACA CCAGAACTTC TTATAGAATT
    GGAAATTCAT GGAGTAAGAC TGACACCAGA GGAAATCTCT TGCAATGTAT CTGTACTGGC
    AATGGGCGAG GAGAGTGGAA ATGTGATAGA CATTCCTCTG CACAAGCAAC TGGTACTGGC
    TCAAACCCCA TCACAAACAT TCAAACTACC CTCTTCCAGC CTGATTCAGA GCTGGAGCCC
    TATGGTCACT GTGTGACTGA CAATGGGGTC TTGTATTCTT TGGGTATGAG ATGGCTGAAG
    GCACAGGGCA GCAAGCAGAT GCTCTGTACC TGCCTTGGTA ATGGTGTGAG CTGTGAAGAA
    ACTGTTGAAA CCATCACATT TGGTGGTAAT GCCAATGGTG AGCCATGTGC CATTCCTTTC
    ACTCATGATG GCAAGACCTA TTATTCCTGC ACAGGAGAGG GTCGTCAGGA TGGAAAATTG
    TGGTGTGCAA CAACCTCCAA CTATGACACT GACAAGAAGT ATTCATTCTG CAATGAGCAG
    CGTGCACTTG TACAGACACG TGGTGGTAAC TCCAATGGAG CTCTTTGCAA CTTTCCATTT
    TTGTACAACA ACCGCAACTA TACAGACTGC ACCTCTGAAG GTCGGAGGGA CAGTATGAAG
    TGGTGCGGGA CTACTGCCAA CTATGATGCC GATCAAAAAT TTGGATTCTG TCCCATGGCA
    GCTCATGAGG AAATATGCAC TACTAATGAG GGAGTCATGT ATAGAGTTGG TGATCAGTGG
    GACAAGCAGC ATGATCAAGG CCACATGATG CGATGTACTT GTGTTGGAAA TGGTCGTGGG
    GAATGGACTT GTGTGGCTTA TTCTCAGCTT AAAGACCAGT GCATTGTGGA TGGGATAACT
    TATGATGTGA ACAGTTCTTT TACGAAACGC CATGAGGAAG GACACATGAT GAACTGTACC
    TGCTATGGGC AGGGTCGTGG CAGATGGAAG TGCGATGCTA TAGACCAATG CCAGGATACT
    GAAACCCGAC AGTTCTATCA GATTGGAGAT TCCTGGGAAA AGCATTTGCA GGGAGTACGG
    TACCAGTGTT ACTGCTATGG CAAAGGAATT GGTGAATGGC ATTGCCAGCC TCTATCAACA
    TCCCCAGCTG GGACTGGACC CGTTCAGGTT ATTATCACAG AGAGTTCCAA TTTCCCTAAT
    TCTCACCCTA TACAGTGGAA TGCCCCACAG CCATCACACA TTAAGAATTA CATCCTGCGC
    TGGAAGCCTA AACTGAAGAC TGGCCCATGG AAACAAGCCA CTATTCCAGG ACACCTGAAC
    TCTTATACCA TCTCTGGCTT GAAACCTGGA ATCCTGTATG AGGGGCAATT GATCAGCATT
    CTGCAGTATG GAAACAGGGA GGTCACAACC TTTGACTTTA CCACCACTTC TACCATTCAC
    CGCTCCAGTC AAACTGAGAC AGGAGAAACT ACCCCACTTC CACCATTGGT GGCCACATCT
    GAGTCTGTAA CAGAAATTAC TGCTAGCAGT TTTCTGGTAT CCTGGGTCTC TGCATCTGAC
    ACAGTGTCTG GCTTCCGTGT TGAATATGAG CTGAGTGAGG ATGGAGATGA GAAACGCTAC
    CTAGAACTTC CAAACACTGC CACTTCAGTC AGTATCCCAG ACCTCCTCCC AGGCCGTAGA
    TACAATGTGA ATGTGTATCA GATAACTGAA GAGGGTGAAA AGAGCCTCAT CCTATCAACT
    ACTCAGACAA CTGCACCTGA TGCACCCCCC GACCATAATG TGGAAAATGT TGATGACACC
    TCCATCCTGA TCAAATGGAC CAAACCTCAA GCTCCCGTCA CAGGATACAG GGTTGTATAC
    AGTCCATCTG TAGAAGGCAG CAGCACAGAG TTAAACCTCC CAAGTACTGC AACTTCAGTG
    AGCCTGACTG AGCTGCTCCC AGGAATTGAA TACAATATTA CAATCTATGC AGTGGAAGAC
    AGTTTAGAAA GTGTTCCCAT CTTTATTCAG CAAGGGACAA CTGGAACTCC ACAGACAGTT
    ATAGTACCAT CTCCCACTGA CTTACAATTA GTGGAAGTTA CCGATATAAA AATTACCATC
    ATGTGGAACC CCCCACAAAG TGAGGTTTCT GGATACCGAG TTGTTGTGAA ACCTGTAAGT
    CCCTCTGACC GTGATGTGCA GAATCTCCCC GTGAGCAGGA ACACTTTTGC CGAGGTGGTT
    AACTTGCACC CTGGCAGGAC CTATACCTTT GAGGTGTATG CAGTTAATCG TGGACAAGAG
    AGTGAACCCC TGGCTGGAGA CTTCACTACC AAACTCGATG CTCCATCAGA CTTGCAGTTT
    ACAGATGTAA CTGACTCTAC AGTTGTTATT ATCTGGAGAC CACCCCAAGC CAAAATTGGT
    CGTTACCTGC TGTCTGTAGG TCAAACCCGG GGAGGCCAGC CGAGCCAATT TCACATTAAC
    CCCTCTGCCA CAAACCACAA GCTGGAGAGT CTCTCCCCAG GCACAGAGTA CACCATTTCA
    CTTGTTGCCC TCAAAGGCAA TCAGCAAAGT GCTAGCACTA CTGGTGTCTT TTCAACATTG
    GAACCTGTGG GATCAATTCC ACCTTACAAC ACTGAAGTCA CAGAGACTAC CATAGTGGTA
    ACTTGGACCC CTGTGCCCAG AATCGGGTTT AAGCTGGATG TTCGACCCAG CCAAGGTGGG
    GAGGCACCTC GGGAAGTCAT ATCCGAGTCT GGCAGCATTG TGATCTCTGG CCTAACCCCT
    GGAGTCGAAT ATATGTACAG CATATCTGTT CTTAAGGATG GCGTTGAGAG GGACATTCCC
    ATCACCAAGA CTGTTGTCAC ACCTGTCGCT CCTCCAACCA ACTTGCGCCT CCAACCAAGC
    AGAGATTCTG CTACACTTAC TGTTTACTGG GACAGTAGCA TTTCACCAGG AATCACTGGA
    TACAGAATTA CCACTGCACC CACTCCAATG CAGGTTGGCA ATTCCCTGGA AGAGGAGGTT
    GGTCCAACTC AGACATACTG CCTCTTTGAG AACCTGAGCC CTGGTGTAGA GTACAATGTC
    AGCGTGTATG CAGTAAAAGG GGAAGAGGAG AGTTCACCTC TCTCCCAGAT CTTTCTTCAA
    GCTGTTCCAC CTCCAACTAA TTTGCGCTTC ACCAATGTTG GCCCTGACAC CATGCGCGTC
    ACCTGGTCAC CTCCAACTTC TATCGAACTA TCAAGTTTCC TGGTACGATA TTCTCCCGTG
    AAGAAGCCAG ATGATGTGAC AGAACTCTCC CTCTCTCCAT CTACCAACAT GGTGGTTTTA
    TCAAACTTGC TTCCCTTCAC CGAATATTTA GTCAGCGTTC ATTCTGTTTA TGAGGAGCGA
    GAGAGTAGTG CCCTCAATGG TGTAGCTAAG ACTTACTTGG ACTCTCCAAC TGGCATTGAC
    TTTTCTGAAA TTACTCCCAA TTCATTTACT GTGCACTGGA TTGCTCCACG TGCCCCTATC
    ACTGGCTACA GAATTCGGTA CCAGTTAGAA AGTGGAGCTG GGCGACCCAA GGAGGAGCGG
    GTACCTCCAT CCCGGAACTC TATTACCCTT ACCCACCTCA TTCCAGGTTC AGAATACATT
    GTCAGTATCA TTGCATTCAA TGGTCAACAG GAGAGTTTGC CACTTTCTGG ACAGCAAGCC
    ACTGTATCTG ATGTCCCAAC TGATTTGGAA GTGACCTCCT CCTCTCCTAA CACACTCACC
    ATCTCTTGGG AAGCACCTTC AGTGAATGTA CGTTACTACA GGATCACCCA TTCCCAGACA
    GGAGGTCATG GTCCAGAGAA GGAGTTTACA GTTCCAGGAA CATCCAACAC TGCAACCATC
    AGAGGGTTAA ATCCAGGAGT AAGCTACACC ATCACTGTTT ATGCAGTGAC TGGGCGAGGG
    GATAGTCCTG CCTCTAGCAA ACCACTTACC ATTGTTCACA TGACAGATGT TGACCAACCA
    ACTGACATGG CTGTCACAGA TATCCAGGAT CACAGCATTC ATGTCAAATG GTCTCCTCCT
    CCAGGCCCAG TCACTGGTTA CAGGGTCACT AGTGTCCCAA AAAGTGGCCA AGGAGAAACC
    TTTTCCCAGG TCGTTTCTCC AGATCAAACT GAAGTGACGA TTGTGGGCCT GCAACCAGCT
    GTGGAGTATG TGGTGAGCAT ATACTCACAA GGTGAAAATG GCGAGAGCGA GCCTCTGGTT
    GAAACTGCAG TTACAAACAT TGACCATCCC AAAGGACTGA CATTCACTGA TGTGGGTGTT
    GATTCCATTA GACTGGCTTG GGAGGTCCCA GATGGGCAGG TCACCCGCTA CAGGGTTACC
    TACTCAAGTC CTGAGGAGGG AGTAAAAGAG TTGTTCCCAG CTCCAGAAGG GGATGATGAC
    ACAGCAGAAT TGCACGGTCT GAGGCCGGGC ACAGAATACA CTGTGAGCAT CGTGGCATTG
    CACGATGACA TGGAGAGCAA GCCGATGATT GGAATCCAGA GCACAGCAAT TCCAGCACCA
    ACAAATCTTC AGTTCTCTCA AGTGACACCA AGTGGCTTCT CTCTTAGCTG GCATGCACCC
    ACTATCCATC TCAGTGGGTA CCTTGTACGT GTCAACCCTA AAGAGAAAAC TGGGCCTATG
    AAGGAAGTTA GACTTTCACC TGGTGTGACT GCTACAACTG TAACTGGTCT GATGGTGGCC
    ACAAAATATG AAGTTAATGT GTATGCTCTT AAGGATACCC TGACAAGTCA GCCACTGCAG
    GGTTTAATCT CTACACTTGA TAATGTAAGT CCACCTCGAA GACCTCGTGT TCAAGATGTG
    ACCGAGACCA CAGTTACACT GTCCTGGCGC ACTAAAACAG AGACCATCAC TGGCTTCCAG
    ATTGATGCTA TACCTGCCGG AGGTCAGAAT CCCATCAGGA GAACAGTTGA TGCAGATCTC
    CGTTCTTTTA CTATCACAGG TCTGCAGCCT GGCACAGACT ACAAGATCTA TCTGTACACC
    CTAAATGACA ATGCTCGTAG CTCTCCTGTG ATTGTTGATG TCACCACAGC TGTGGACTCT
    CCATCCAACC TGCGTTTCCT GACCACAACT AGCAACTCCC TGCTGTTCAC TTGGCAGCCA
    CCTCGTGCTC GCATCACTGG CTATATCATT CGTTATGAGA AAGCTGGAGG GCTACTGAAA
    GAGCACCTCC CACGCCTCCA AGCAGGAACC ACAGAGTCTA CCATTACCAA CCTGGAGCCA
    GGCACAGAAT ATATTGTCTA TATTATTGCT GTGAGAAACA ATCAGAAGAG TGAGCCTCTT
    GTGGGACGTA AGAGAACAGA CGAACTGCCT CGCCTGGTTA CACTTCCACA CCCGGGCAAA
    GGCCCTGAAA TCCTTGATGT TCCCACTGAT GAAGAGAACA CACCCCACAT CACACAAATC
    AAGTTGGACA ATGGTAATGG TATCCAACTG CCAGGCTCTA ATGGACAGCA GCCCAGTTCT
    GGCAATGAAG GGCAGCTTAT AGAAGAACAT GGTTTCAGAA GTCCCCTTGC ACCCACTACT
    GCTGTGCCAG TGAGGCCAGG GAAATTCACC CCTGGGCACT ACCCACAGGA GAGGGTTGAT
    ATTGAGCTAG ACACAGAGTT CCCTGTGCAG CGTGGAGATC TTGATGGTCC TTACCCTCAT
    GGCTTAGGAC CACAGCTAAA TGATTCTGGC GTCCAAGAAG TGGCATCCCA TACTACCATT
    TCATGGAGAC CTGAGCTGGA AACCACTGAA TATATCATCT CTTGCCATCC TATTGAACAT
    GATGAGGCCC CATTGCAGTT TAGGGTCCCT GGAACCTCCT CTAGTGCCAC ATTAAATGGT
    CTGACTCAAG GAGCCACTTA TAACATCATT GTGGAAGCGC AGAAGGGGTC TGATAAACAT
    AAGGTCTTCG AGAAGCGTGT AACTGTTGGC AGTCCTGGTT CCCCTGAGGG GGTTTTACAG
    CCAGCTGAGG ACACCTGTTA TGACACATTC AATGGAGCAC ACTATTCTGT GGGACAGGAA
    TGGGAACGCA TGTCAGAATC TGGATTCAGG CTCTGGTGCA AGTGCCTGGG CTATGGAAGT
    GGACACTTCA GATGTGACTC CTCCAAATGG TGCCATGATA ATGGTGTCAA TCACAGGGTC
    GGGGAGAAAT GGGACCGACG AGGAGAGAAT GGTCAGATGA TGAGTTGTAC TTGTCTTGGC
    AATGGAAAAG GAGAATTTAA GTGTGAACCT CATGAAGCCA CGTGCTATGA TGAAGGGAAG
    ATGTACAATG TTGGAGAGCA GTGGCAGAAG GAATATTTAG GGGCAATCTG TTCATGCACA
    TGCTATGGAG GACAGCAAGG GTGGAGATGT GACAATTGCC GCAGACCAGG TGCTGTTCTT
    CCCGATGGCA CTGCTGGGCA TACAGTCTCT CAGTTTGCCC AGAGATACCA ACAGAACTAT
    AATTTGAACT GCCCCATTGA GTGCTACCTT CCCCTCCAAG CAGATATACA GCATTCGGAA
    CAGACCCAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825948.1
    CDS222..7376
    Translation

    Target ORF information:

    RefSeq Version XM_012970494.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis fibronectin 1 (fn1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970494.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCGCTGGG GGGCCCTGAC CGGGCTGCTC CTAGCCCTGT GCCTGTGTGC AGTGGTACGT 
    TCCGCCCCAT CAAGCAAGAA GCGCAGGCAG GCGCAGCAGC AGCAAGTAGT GCAACCCCAT
    GGATCACCGG ACAGCCAGCA GAAAGGATGT TATGACAATG GCAAGTACTA CCAAATCAAC
    CAGCAATGGG AGCGCACCTA CCTAGGGAAC ACACTGGTGT GTACTTGTTA TGGCGGAGGC
    AGAGGTTTCA ACTGCGAAAG CAAACCAGAG TCTGAAGAGA CCTGCTTTGA CAAATACACT
    GGTGTTACTT ACCGGGTCGG TGAAACATAT GAGCGACCAA AGGATAACAT GATCTGGGAC
    TGTACGTGTA TTGGAGCTGG AAGGGGACGC ATTAGCTGTA CAATTGCAAA TCGTTGCCAT
    GAAGGAGGTC AGTCTTATAA GATTGGGGAC ACCTGGCGCA GGCCACATGA AACTGGGGGG
    TATATGCTAG AATGTGTCTG CCTTGGAAAT GGTAAAGGAG AATGGACCTG CAAGCCCGTT
    GCTGAGAGAT GCTATGATAA CACAGCTGGG ATATCCTATG TAGTTGGTCA GACCTGGGAA
    AAGCCTTATC AGGGCTGGAT GATGGTGGAC TGTACATGCT TGGGAGAAGG CAGTGGACGA
    ATCACATGTT CTTCCAAAAA CAGATGCAAT GACCAGGACA CCAGAACTTC TTATAGAATT
    GGAAATTCAT GGAGTAAGAC TGACACCAGA GGAAATCTCT TGCAATGTAT CTGTACTGGC
    AATGGGCGAG GAGAGTGGAA ATGTGATAGA CATTCCTCTG CACAAGCAAC TGGTACTGGC
    TCAAACCCCA TCACAAACAT TCAAACTACC CTCTTCCAGC CTGATTCAGA GCTGGAGCCC
    TATGGTCACT GTGTGACTGA CAATGGGGTC TTGTATTCTT TGGGTATGAG ATGGCTGAAG
    GCACAGGGCA GCAAGCAGAT GCTCTGTACC TGCCTTGGTA ATGGTGTGAG CTGTGAAGAA
    ACTGTTGAAA CCATCACATT TGGTGGTAAT GCCAATGGTG AGCCATGTGC CATTCCTTTC
    ACTCATGATG GCAAGACCTA TTATTCCTGC ACAGGAGAGG GTCGTCAGGA TGGAAAATTG
    TGGTGTGCAA CAACCTCCAA CTATGACACT GACAAGAAGT ATTCATTCTG CAATGAGCAG
    CGTGCACTTG TACAGACACG TGGTGGTAAC TCCAATGGAG CTCTTTGCAA CTTTCCATTT
    TTGTACAACA ACCGCAACTA TACAGACTGC ACCTCTGAAG GTCGGAGGGA CAGTATGAAG
    TGGTGCGGGA CTACTGCCAA CTATGATGCC GATCAAAAAT TTGGATTCTG TCCCATGGCA
    GCTCATGAGG AAATATGCAC TACTAATGAG GGAGTCATGT ATAGAGTTGG TGATCAGTGG
    GACAAGCAGC ATGATCAAGG CCACATGATG CGATGTACTT GTGTTGGAAA TGGTCGTGGG
    GAATGGACTT GTGTGGCTTA TTCTCAGCTT AAAGACCAGT GCATTGTGGA TGGGATAACT
    TATGATGTGA ACAGTTCTTT TACGAAACGC CATGAGGAAG GACACATGAT GAACTGTACC
    TGCTATGGGC AGGGTCGTGG CAGATGGAAG TGCGATGCTA TAGACCAATG CCAGGATACT
    GAAACCCGAC AGTTCTATCA GATTGGAGAT TCCTGGGAAA AGCATTTGCA GGGAGTACGG
    TACCAGTGTT ACTGCTATGG CAAAGGAATT GGTGAATGGC ATTGCCAGCC TCTATCAACA
    TCCCCAGCTG GGACTGGACC CGTTCAGGTT ATTATCACAG AGAGTTCCAA TTTCCCTAAT
    TCTCACCCTA TACAGTGGAA TGCCCCACAG CCATCACACA TTAAGAATTA CATCCTGCGC
    TGGAAGCCTA AACTGAAGAC TGGCCCATGG AAACAAGCCA CTATTCCAGG ACACCTGAAC
    TCTTATACCA TCTCTGGCTT GAAACCTGGA ATCCTGTATG AGGGGCAATT GATCAGCATT
    CTGCAGTATG GAAACAGGGA GGTCACAACC TTTGACTTTA CCACCACTTC TACCATTCAC
    CGCTCCAGTC AAACTGAGAC AGGAGAAACT ACCCCACTTC CACCATTGGT GGCCACATCT
    GAGTCTGTAA CAGAAATTAC TGCTAGCAGT TTTCTGGTAT CCTGGGTCTC TGCATCTGAC
    ACAGTGTCTG GCTTCCGTGT TGAATATGAG CTGAGTGAGG ATGGAGATGA GAAACGCTAC
    CTAGAACTTC CAAACACTGC CACTTCAGTC AGTATCCCAG ACCTCCTCCC AGGCCGTAGA
    TACAATGTGA ATGTGTATCA GATAACTGAA GAGGGTGAAA AGAGCCTCAT CCTATCAACT
    ACTCAGACAA CTGCACCTGA TGCACCCCCC GACCATAATG TGGAAAATGT TGATGACACC
    TCCATCCTGA TCAAATGGAC CAAACCTCAA GCTCCCGTCA CAGGATACAG GGTTGTATAC
    AGTCCATCTG TAGAAGGCAG CAGCACAGAG TTAAACCTCC CAAGTACTGC AACTTCAGTG
    AGCCTGACTG AGCTGCTCCC AGGAATTGAA TACAATATTA CAATCTATGC AGTGGAAGAC
    AGTTTAGAAA GTGTTCCCAT CTTTATTCAG CAAGGGACAA CTGGAACTCC ACAGACAGTT
    ATAGTACCAT CTCCCACTGA CTTACAATTA GTGGAAGTTA CCGATATAAA AATTACCATC
    ATGTGGAACC CCCCACAAAG TGAGGTTTCT GGATACCGAG TTGTTGTGAA ACCTGTAAGT
    CCCTCTGACC GTGATGTGCA GAATCTCCCC GTGAGCAGGA ACACTTTTGC CGAGGTGGTT
    AACTTGCACC CTGGCAGGAC CTATACCTTT GAGGTGTATG CAGTTAATCG TGGACAAGAG
    AGTGAACCCC TGGCTGGAGA CTTCACTACC AAACTCGATG CTCCATCAGA CTTGCAGTTT
    ACAGATGTAA CTGACTCTAC AGTTGTTATT ATCTGGAGAC CACCCCAAGC CAAAATTGGT
    CGTTACCTGC TGTCTGTAGG TCAAACCCGG GGAGGCCAGC CGAGCCAATT TCACATTAAC
    CCCTCTGCCA CAAACCACAA GCTGGAGAGT CTCTCCCCAG GCACAGAGTA CACCATTTCA
    CTTGTTGCCC TCAAAGGCAA TCAGCAAAGT GCTAGCACTA CTGGTGTCTT TTCAACATTG
    GAACCTGTGG GATCAATTCC ACCTTACAAC ACTGAAGTCA CAGAGACTAC CATAGTGGTA
    ACTTGGACCC CTGTGCCCAG AATCGGGTTT AAGCTGGATG TTCGACCCAG CCAAGGTGGG
    GAGGCACCTC GGGAAGTCAT ATCCGAGTCT GGCAGCATTG TGATCTCTGG CCTAACCCCT
    GGAGTCGAAT ATATGTACAG CATATCTGTT CTTAAGGATG GCGTTGAGAG GGACATTCCC
    ATCACCAAGA CTGTTGTCAC ACCTGTCGCT CCTCCAACCA ACTTGCGCCT CCAACCAAGC
    AGAGATTCTG CTACACTTAC TGTTTACTGG GACAGTAGCA TTTCACCAGG AATCACTGGA
    TACAGAATTA CCACTGCACC CACTCCAATG CAGGTTGGCA ATTCCCTGGA AGAGGAGGTT
    GGTCCAACTC AGACATACTG CCTCTTTGAG AACCTGAGCC CTGGTGTAGA GTACAATGTC
    AGCGTGTATG CAGTAAAAGG GGAAGAGGAG AGTTCACCTC TCTCCCAGAT CTTTCTTCAA
    GCTGTTCCAC CTCCAACTAA TTTGCGCTTC ACCAATGTTG GCCCTGACAC CATGCGCGTC
    ACCTGGTCAC CTCCAACTTC TATCGAACTA TCAAGTTTCC TGGTACGATA TTCTCCCGTG
    AAGAAGCCAG ATGATGTGAC AGAACTCTCC CTCTCTCCAT CTACCAACAT GGTGGTTTTA
    TCAAACTTGC TTCCCTTCAC CGAATATTTA GTCAGCGTTC ATTCTGTTTA TGAGGAGCGA
    GAGAGTAGTG CCCTCAATGG TGTAGCTAAG ACTTACTTGG ACTCTCCAAC TGGCATTGAC
    TTTTCTGAAA TTACTCCCAA TTCATTTACT GTGCACTGGA TTGCTCCACG TGCCCCTATC
    ACTGGCTACA GAATTCGGTA CCAGTTAGAA AGTGGAGCTG GGCGACCCAA GGAGGAGCGG
    GTACCTCCAT CCCGGAACTC TATTACCCTT ACCCACCTCA TTCCAGGTTC AGAATACATT
    GTCAGTATCA TTGCATTCAA TGGTCAACAG GAGAGTTTGC CACTTTCTGG ACAGCAAGCC
    ACTGTATCTG ATGTCCCAAC TGATTTGGAA GTGACCTCCT CCTCTCCTAA CACACTCACC
    ATCTCTTGGG AAGCACCTTC AGTGAATGTA CGTTACTACA GGATCACCCA TTCCCAGACA
    GGAGGTCATG GTCCAGAGAA GGAGTTTACA GTTCCAGGAA CATCCAACAC TGCAACCATC
    AGAGGGTTAA ATCCAGGAGT AAGCTACACC ATCACTGTTT ATGCAGTGAC TGGGCGAGGG
    GATAGTCCTG CCTCTAGCAA ACCACTTACC ATTGTTCACA TGACAGATGT TGACCAACCA
    ACTGACATGG CTGTCACAGA TATCCAGGAT CACAGCATTC ATGTCAAATG GTCTCCTCCT
    CCAGGCCCAG TCACTGGTTA CAGGGTCACT AGTGTCCCAA AAAGTGGCCA AGGAGAAACC
    TTTTCCCAGG TCGTTTCTCC AGATCAAACT GAAGTGACGA TTGTGGGCCT GCAACCAGCT
    GTGGAGTATG TGGTGAGCAT ATACTCACAA GGTGAAAATG GCGAGAGCGA GCCTCTGGTT
    GAAACTGCAG TTACAAACAT TGACCATCCC AAAGGACTGA CATTCACTGA TGTGGGTGTT
    GATTCCATTA GACTGGCTTG GGAGGTCCCA GATGGGCAGG TCACCCGCTA CAGGGTTACC
    TACTCAAGTC CTGAGGAGGG AGTAAAAGAG TTGTTCCCAG CTCCAGAAGG GGATGATGAC
    ACAGCAGAAT TGCACGGTCT GAGGCCGGGC ACAGAATACA CTGTGAGCAT CGTGGCATTG
    CACGATGACA TGGAGAGCAA GCCGATGATT GGAATCCAGA GCACAGCAAT TCCAGCACCA
    ACAAATCTTC AGTTCTCTCA AGTGACACCA AGTGGCTTCT CTCTTAGCTG GCATGCACCC
    ACTATCCATC TCAGTGGGTA CCTTGTACGT GTCAACCCTA AAGAGAAAAC TGGGCCTATG
    AAGGAAGTTA GACTTTCACC TGGTGTGACT GCTACAACTG TAACTGGTCT GATGGTGGCC
    ACAAAATATG AAGTTAATGT GTATGCTCTT AAGGATACCC TGACAAGTCA GCCACTGCAG
    GGTTTAATCT CTACACTTGA TAATGTAAGT CCACCTCGAA GACCTCGTGT TCAAGATGTG
    ACCGAGACCA CAGTTACACT GTCCTGGCGC ACTAAAACAG AGACCATCAC TGGCTTCCAG
    ATTGATGCTA TACCTGCCGG AGGTCAGAAT CCCATCAGGA GAACAGTTGA TGCAGATCTC
    CGTTCTTTTA CTATCACAGG TCTGCAGCCT GGCACAGACT ACAAGATCTA TCTGTACACC
    CTAAATGACA ATGCTCGTAG CTCTCCTGTG ATTGTTGATG TCACCACAGC TGTGGACTCT
    CCATCCAACC TGCGTTTCCT GACCACAACT AGCAACTCCC TGCTGTTCAC TTGGCAGCCA
    CCTCGTGCTC GCATCACTGG CTATATCATT CGTTATGAGA AAGCTGGAGG GCTACTGAAA
    GAGCACCTCC CACGCCTCCA AGCAGGAACC ACAGAGTCTA CCATTACCAA CCTGGAGCCA
    GGCACAGAAT ATATTGTCTA TATTATTGCT GTGAGAAACA ATCAGAAGAG TGAGCCTCTT
    GTGGGACGTA AGAGAACAGA CGAACTGCCT CGCCTGGTTA CACTTCCACA CCCGGGCAAA
    GGCCCTGAAA TCCTTGATGT TCCCACTGAT GAAGAGAACA CACCCCACAT CACACAAATC
    AAGTTGGACA ATGGTAATGG TATCCAACTG CCAGGCTCTA ATGGACAGCA GCCCAGTTCT
    GGCAATGAAG GGCAGCTTAT AGAAGAACAT GGTTTCAGAA GTCCCCTTGC ACCCACTACT
    GCTGTGCCAG TGAGGCCAGG GAAATTCACC CCTGGGCACT ACCCACAGGA GAGGGTTGAT
    ATTGAGCTAG ACACAGAGTT CCCTGTGCAG CGTGGAGATC TTGATGGTCC TTACCCTCAT
    GGCTTAGGAC CACAGCTAAA TGATTCTGGC GTCCAAGAAG TGGCATCCCA TACTACCATT
    TCATGGAGAC CTGAGCTGGA AACCACTGAA TATATCATCT CTTGCCATCC TATTGAACAT
    GATGAGGCCC CATTGCAGTT TAGGGTCCCT GGAACCTCCT CTAGTGCCAC ATTAAATGGT
    CTGACTCAAG GAGCCACTTA TAACATCATT GTGGAAGCGC AGAAGGGGTC TGATAAACAT
    AAGGTCTTCG AGAAGCGTGT AACTGTTGGC AGTCCTGGTT CCCCTGAGGG GGTTTTACAG
    CCAGCTGAGG ACACCTGTTA TGACACATTC AATGGAGCAC ACTATTCTGT GGGACAGGAA
    TGGGAACGCA TGTCAGAATC TGGATTCAGG CTCTGGTGCA AGTGCCTGGG CTATGGAAGT
    GGACACTTCA GATGTGACTC CTCCAAATGG TGCCATGATA ATGGTGTCAA TCACAGGGTC
    GGGGAGAAAT GGGACCGACG AGGAGAGAAT GGTCAGATGA TGAGTTGTAC TTGTCTTGGC
    AATGGAAAAG GAGAATTTAA GTGTGAACCT CATGAAGCCA CGTGCTATGA TGAAGGGAAG
    ATGTACAATG TTGGAGAGCA GTGGCAGAAG GAATATTTAG GGGCAATCTG TTCATGCACA
    TGCTATGGAG GACAGCAAGG GTGGAGATGT GACAATTGCC GCAGACCAGG TGCTGTTCTT
    CCCGATGGCA CTGCTGGGCA TACAGTCTCT CAGTTTGCCC AGAGATACCA ACAGAACTAT
    AATTTGAACT GCCCCATTGA GTGCTACCTT CCCCTCCAAG CAGATATACA GCATTCGGAA
    CAGACCCAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa28346
    Clone ID Related Accession (Same CDS sequence) XM_012970495.3 , XM_012970495.2
    Accession Version XM_012970495.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product fibronectin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970495.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
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    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
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    3421
    3481
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    4081
    4141
    4201
    4261
    4321
    4381
    4441
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    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCGCTGGG GGGCCCTGAC CGGGCTGCTC CTAGCCCTGT GCCTGTGTGC AGTGGTACGT 
    TCCGCCCCAT CAAGCAAGAA GCGCAGGCAG GCGCAGCAGC AGCAAGTAGT GCAACCCCAT
    GGATCACCGG ACAGCCAGCA GAAAGGATGT TATGACAATG GCAAGTACTA CCAAATCAAC
    CAGCAATGGG AGCGCACCTA CCTAGGGAAC ACACTGGTGT GTACTTGTTA TGGCGGAGGC
    AGAGGTTTCA ACTGCGAAAG CAAACCAGAG TCTGAAGAGA CCTGCTTTGA CAAATACACT
    GGTGTTACTT ACCGGGTCGG TGAAACATAT GAGCGACCAA AGGATAACAT GATCTGGGAC
    TGTACGTGTA TTGGAGCTGG AAGGGGACGC ATTAGCTGTA CAATTGCAAA TCGTTGCCAT
    GAAGGAGGTC AGTCTTATAA GATTGGGGAC ACCTGGCGCA GGCCACATGA AACTGGGGGG
    TATATGCTAG AATGTGTCTG CCTTGGAAAT GGTAAAGGAG AATGGACCTG CAAGCCCGTT
    GCTGAGAGAT GCTATGATAA CACAGCTGGG ATATCCTATG TAGTTGGTCA GACCTGGGAA
    AAGCCTTATC AGGGCTGGAT GATGGTGGAC TGTACATGCT TGGGAGAAGG CAGTGGACGA
    ATCACATGTT CTTCCAAAAA CAGATGCAAT GACCAGGACA CCAGAACTTC TTATAGAATT
    GGAAATTCAT GGAGTAAGAC TGACACCAGA GGAAATCTCT TGCAATGTAT CTGTACTGGC
    AATGGGCGAG GAGAGTGGAA ATGTGATAGA CATTCCTCTG CACAAGCAAC TGGTACTGGC
    TCAAACCCCA TCACAAACAT TCAAACTACC CTCTTCCAGC CTGATTCAGA GCTGGAGCCC
    TATGGTCACT GTGTGACTGA CAATGGGGTC TTGTATTCTT TGGGTATGAG ATGGCTGAAG
    GCACAGGGCA GCAAGCAGAT GCTCTGTACC TGCCTTGGTA ATGGTGTGAG CTGTGAAGAA
    ACTGTTGAAA CCATCACATT TGGTGGTAAT GCCAATGGTG AGCCATGTGC CATTCCTTTC
    ACTCATGATG GCAAGACCTA TTATTCCTGC ACAGGAGAGG GTCGTCAGGA TGGAAAATTG
    TGGTGTGCAA CAACCTCCAA CTATGACACT GACAAGAAGT ATTCATTCTG CAATGAGCAG
    CGTGCACTTG TACAGACACG TGGTGGTAAC TCCAATGGAG CTCTTTGCAA CTTTCCATTT
    TTGTACAACA ACCGCAACTA TACAGACTGC ACCTCTGAAG GTCGGAGGGA CAGTATGAAG
    TGGTGCGGGA CTACTGCCAA CTATGATGCC GATCAAAAAT TTGGATTCTG TCCCATGGCA
    GCTCATGAGG AAATATGCAC TACTAATGAG GGAGTCATGT ATAGAGTTGG TGATCAGTGG
    GACAAGCAGC ATGATCAAGG CCACATGATG CGATGTACTT GTGTTGGAAA TGGTCGTGGG
    GAATGGACTT GTGTGGCTTA TTCTCAGCTT AAAGACCAGT GCATTGTGGA TGGGATAACT
    TATGATGTGA ACAGTTCTTT TACGAAACGC CATGAGGAAG GACACATGAT GAACTGTACC
    TGCTATGGGC AGGGTCGTGG CAGATGGAAG TGCGATGCTA TAGACCAATG CCAGGATACT
    GAAACCCGAC AGTTCTATCA GATTGGAGAT TCCTGGGAAA AGCATTTGCA GGGAGTACGG
    TACCAGTGTT ACTGCTATGG CAAAGGAATT GGTGAATGGC ATTGCCAGCC TCTATCAACA
    TCCCCAGCTG GGACTGGACC CGTTCAGGTT ATTATCACAG AGAGTTCCAA TTTCCCTAAT
    TCTCACCCTA TACAGTGGAA TGCCCCACAG CCATCACACA TTAAGAATTA CATCCTGCGC
    TGGAAGCCTA AACTGAAGAC TGGCCCATGG AAACAAGCCA CTATTCCAGG ACACCTGAAC
    TCTTATACCA TCTCTGGCTT GAAACCTGGA ATCCTGTATG AGGGGCAATT GATCAGCATT
    CTGCAGTATG GAAACAGGGA GGTCACAACC TTTGACTTTA CCACCACTTC TACCATTCAC
    CGCTCCAGTC AAACTGAGAC AGGAGAAACT ACCCCACTTC CACCATTGGT GGCCACATCT
    GAGTCTGTAA CAGAAATTAC TGCTAGCAGT TTTCTGGTAT CCTGGGTCTC TGCATCTGAC
    ACAGTGTCTG GCTTCCGTGT TGAATATGAG CTGAGTGAGG ATGGAGATGA GAAACGCTAC
    CTAGAACTTC CAAACACTGC CACTTCAGTC AGTATCCCAG ACCTCCTCCC AGGCCGTAGA
    TACAATGTGA ATGTGTATCA GATAACTGAA GAGGGTGAAA AGAGCCTCAT CCTATCAACT
    ACTCAGACAA CTGCACCTGA TGCACCCCCC GACCATAATG TGGAAAATGT TGATGACACC
    TCCATCCTGA TCAAATGGAC CAAACCTCAA GCTCCCGTCA CAGGATACAG GGTTGTATAC
    AGTCCATCTG TAGAAGGCAG CAGCACAGAG TTAAACCTCC CAAGTACTGC AACTTCAGTG
    AGCCTGACTG AGCTGCTCCC AGGAATTGAA TACAATATTA CAATCTATGC AGTGGAAGAC
    AGTTTAGAAA GTGTTCCCAT CTTTATTCAG CAAGGGACAA CTGGAACTCC ACAGACAGTT
    ATAGTACCAT CTCCCACTGA CTTACAATTA GTGGAAGTTA CCGATATAAA AATTACCATC
    ATGTGGAACC CCCCACAAAG TGAGGTTTCT GGATACCGAG TTGTTGTGAA ACCTGTAAGT
    CCCTCTGACC GTGATGTGCA GAATCTCCCC GTGAGCAGGA ACACTTTTGC CGAGGTGGTT
    AACTTGCACC CTGGCAGGAC CTATACCTTT GAGGTGTATG CAGTTAATCG TGGACAAGAG
    AGTGAACCCC TGGCTGGAGA CTTCACTACC AAACTCGATG CTCCATCAGA CTTGCAGTTT
    ACAGATGTAA CTGACTCTAC AGTTGTTATT ATCTGGAGAC CACCCCAAGC CAAAATTGGT
    CGTTACCTGC TGTCTGTAGG TCAAACCCGG GGAGGCCAGC CGAGCCAATT TCACATTAAC
    CCCTCTGCCA CAAACCACAA GCTGGAGAGT CTCTCCCCAG GCACAGAGTA CACCATTTCA
    CTTGTTGCCC TCAAAGGCAA TCAGCAAAGT GCTAGCACTA CTGGTGTCTT TTCAACATTG
    GAACCTGTGG GATCAATTCC ACCTTACAAC ACTGAAGTCA CAGAGACTAC CATAGTGGTA
    ACTTGGACCC CTGTGCCCAG AATCGGGTTT AAGCTGGATG TTCGACCCAG CCAAGGTGGG
    GAGGCACCTC GGGAAGTCAT ATCCGAGTCT GGCAGCATTG TGATCTCTGG CCTAACCCCT
    GGAGTCGAAT ATATGTACAG CATATCTGTT CTTAAGGATG GCGTTGAGAG GGACATTCCC
    ATCACCAAGA CTGTTGTCAC ACCTGTCGCT CCTCCAACCA ACTTGCGCCT CCAACCAAGC
    AGAGATTCTG CTACACTTAC TGTTTACTGG GACAGTAGCA TTTCACCAGG AATCACTGGA
    TACAGAATTA CCACTGCACC CACTCCAATG CAGGTTGGCA ATTCCCTGGA AGAGGAGGTT
    GGTCCAACTC AGACATACTG CCTCTTTGAG AACCTGAGCC CTGGTGTAGA GTACAATGTC
    AGCGTGTATG CAGTAAAAGG GGAAGAGGAG AGTTCACCTC TCTCCCAGAT CTTTCTTCAA
    GAGATACCCC AACTGACTGA CCTAAAGTAT GACGATGTAA CTGACACAAG CATTGACCTG
    AGGTGGACGC CCCTTAATTC CTCCACCATT ATCGGCTACC GAATCACGGT GGTTGCGGCG
    GGAGAATCCG TCCCTATCTA CGAGGAATTT GTGGGCCCCA CAGATGGGTA TTACAAAGTT
    TCAGGATTGG AACCCGGCAT TGACTATGAG ATCAGCGTGA TAACACTTAT TAGCGGCGGA
    GAGAGCGCCC CGACCACAAT CGTACAGCAC ACGGCTGTTC CACCTCCAAC TAATTTGCGC
    TTCACCAATG TTGGCCCTGA CACCATGCGC GTCACCTGGT CACCTCCAAC TTCTATCGAA
    CTATCAAGTT TCCTGGTACG ATATTCTCCC GTGAAGAAGC CAGATGATGT GACAGAACTC
    TCCCTCTCTC CATCTACCAA CATGGTGGTT TTATCAAACT TGCTTCCCTT CACCGAATAT
    TTAGTCAGCG TTCATTCTGT TTATGAGGAG CGAGAGAGTA GTGCCCTCAA TGGTGTAGCT
    AAGACTTACT TGGACTCTCC AACTGGCATT GACTTTTCTG AAATTACTCC CAATTCATTT
    ACTGTGCACT GGATTGCTCC ACGTGCCCCT ATCACTGGCT ACAGAATTCG GTACCAGTTA
    GAAAGTGGAG CTGGGCGACC CAAGGAGGAG CGGGTACCTC CATCCCGGAA CTCTATTACC
    CTTACCCACC TCATTCCAGG TTCAGAATAC ATTGTCAGTA TCATTGCATT CAATGGTCAA
    CAGGAGAGTT TGCCACTTTC TGGACAGCAA GCCACTGTAT CTGATGTCCC AACTGATTTG
    GAAGTGACCT CCTCCTCTCC TAACACACTC ACCATCTCTT GGGAAGCACC TTCAGTGAAT
    GTACGTTACT ACAGGATCAC CCATTCCCAG ACAGGAGGTC ATGGTCCAGA GAAGGAGTTT
    ACAGTTCCAG GAACATCCAA CACTGCAACC ATCAGAGGGT TAAATCCAGG AGTAAGCTAC
    ACCATCACTG TTTATGCAGT GACTGGGCGA GGGGATAGTC CTGCCTCTAG CAAACCACTT
    ACCATTGTTC ACATGACAGA TGTTGACCAA CCAACTGACA TGGCTGTCAC AGATATCCAG
    GATCACAGCA TTCATGTCAA ATGGTCTCCT CCTCCAGGCC CAGTCACTGG TTACAGGGTC
    ACTAGTGTCC CAAAAAGTGG CCAAGGAGAA ACCTTTTCCC AGGTCGTTTC TCCAGATCAA
    ACTGAAGTGA CGATTGTGGG CCTGCAACCA GCTGTGGAGT ATGTGGTGAG CATATACTCA
    CAAGGTGAAA ATGGCGAGAG CGAGCCTCTG GTTGAAACTG CAGTTACAAA CATTGACCAT
    CCCAAAGGAC TGACATTCAC TGATGTGGGT GTTGATTCCA TTAGACTGGC TTGGGAGGTC
    CCAGATGGGC AGGTCACCCG CTACAGGGTT ACCTACTCAA GTCCTGAGGA GGGAGTAAAA
    GAGTTGTTCC CAGCTCCAGA AGGGGATGAT GACACAGCAG AATTGCACGG TCTGAGGCCG
    GGCACAGAAT ACACTGTGAG CATCGTGGCA TTGCACGATG ACATGGAGAG CAAGCCGATG
    ATTGGAATCC AGAGCACAGC AATTCCAGCA CCAACAAATC TTCAGTTCTC TCAAGTGACA
    CCAAGTGGCT TCTCTCTTAG CTGGCATGCA CCCACTATCC ATCTCAGTGG GTACCTTGTA
    CGTGTCAACC CTAAAGAGAA AACTGGGCCT ATGAAGGAAG TTAGACTTTC ACCTGGTGTG
    ACTGCTACAA CTGTAACTGG TCTGATGGTG GCCACAAAAT ATGAAGTTAA TGTGTATGCT
    CTTAAGGATA CCCTGACAAG TCAGCCACTG CAGGGTTTAA TCTCTACACT TGATAATGTA
    AGTCCACCTC GAAGACCTCG TGTTCAAGAT GTGACCGAGA CCACAGTTAC ACTGTCCTGG
    CGCACTAAAA CAGAGACCAT CACTGGCTTC CAGATTGATG CTATACCTGC CGGAGGTCAG
    AATCCCATCA GGAGAACAGT TGATGCAGAT CTCCGTTCTT TTACTATCAC AGGTCTGCAG
    CCTGGCACAG ACTACAAGAT CTATCTGTAC ACCCTAAATG ACAATGCTCG TAGCTCTCCT
    GTGATTGTTG ATGTCACCAC AGCTGTGGAC TCTCCATCCA ACCTGCGTTT CCTGACCACA
    ACTAGCAACT CCCTGCTGTT CACTTGGCAG CCACCTCGTG CTCGCATCAC TGGCTATATC
    ATTCGTTATG AGAAAGCTGG AGGGCTACTG AAAGAGCACC TCCCACGCCT CCAAGCAGGA
    ACCACAGAGT CTACCATTAC CAACCTGGAG CCAGGCACAG AATATATTGT CTATATTATT
    GCTGTGAGAA ACAATCAGAA GAGTGAGCCT CTTGTGGGAC GTAAGAGAAC AGGACCACAG
    CTAAATGATT CTGGCGTCCA AGAAGTGGCA TCCCATACTA CCATTTCATG GAGACCTGAG
    CTGGAAACCA CTGAATATAT CATCTCTTGC CATCCTATTG AACATGATGA GGCCCCATTG
    CAGTTTAGGG TCCCTGGAAC CTCCTCTAGT GCCACATTAA ATGGTCTGAC TCAAGGAGCC
    ACTTATAACA TCATTGTGGA AGCGCAGAAG GGGTCTGATA AACATAAGGT CTTCGAGAAG
    CGTGTAACTG TTGGCAGTCC TGGTTCCCCT GAGGGGGTTT TACAGCCAGC TGAGGACACC
    TGTTATGACA CATTCAATGG AGCACACTAT TCTGTGGGAC AGGAATGGGA ACGCATGTCA
    GAATCTGGAT TCAGGCTCTG GTGCAAGTGC CTGGGCTATG GAAGTGGACA CTTCAGATGT
    GACTCCTCCA AATGGTGCCA TGATAATGGT GTCAATCACA GGGTCGGGGA GAAATGGGAC
    CGACGAGGAG AGAATGGTCA GATGATGAGT TGTACTTGTC TTGGCAATGG AAAAGGAGAA
    TTTAAGTGTG AACCTCATGA AGCCACGTGC TATGATGAAG GGAAGATGTA CAATGTTGGA
    GAGCAGTGGC AGAAGGAATA TTTAGGGGCA ATCTGTTCAT GCACATGCTA TGGAGGACAG
    CAAGGGTGGA GATGTGACAA TTGCCGCAGA CCAGGTGCTG TTCTTCCCGA TGGCACTGCT
    GGGCATACAG TCTCTCAGTT TGCCCAGAGA TACCAACAGA ACTATAATTT GAACTGCCCC
    ATTGAGTGCT ACCTTCCCCT CCAAGCAGAT ATACAGCATT CGGAACAGAC CCAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825949.1
    CDS222..7301
    Translation

    Target ORF information:

    RefSeq Version XM_012970495.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis fibronectin 1 (fn1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970495.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCGCTGGG GGGCCCTGAC CGGGCTGCTC CTAGCCCTGT GCCTGTGTGC AGTGGTACGT 
    TCCGCCCCAT CAAGCAAGAA GCGCAGGCAG GCGCAGCAGC AGCAAGTAGT GCAACCCCAT
    GGATCACCGG ACAGCCAGCA GAAAGGATGT TATGACAATG GCAAGTACTA CCAAATCAAC
    CAGCAATGGG AGCGCACCTA CCTAGGGAAC ACACTGGTGT GTACTTGTTA TGGCGGAGGC
    AGAGGTTTCA ACTGCGAAAG CAAACCAGAG TCTGAAGAGA CCTGCTTTGA CAAATACACT
    GGTGTTACTT ACCGGGTCGG TGAAACATAT GAGCGACCAA AGGATAACAT GATCTGGGAC
    TGTACGTGTA TTGGAGCTGG AAGGGGACGC ATTAGCTGTA CAATTGCAAA TCGTTGCCAT
    GAAGGAGGTC AGTCTTATAA GATTGGGGAC ACCTGGCGCA GGCCACATGA AACTGGGGGG
    TATATGCTAG AATGTGTCTG CCTTGGAAAT GGTAAAGGAG AATGGACCTG CAAGCCCGTT
    GCTGAGAGAT GCTATGATAA CACAGCTGGG ATATCCTATG TAGTTGGTCA GACCTGGGAA
    AAGCCTTATC AGGGCTGGAT GATGGTGGAC TGTACATGCT TGGGAGAAGG CAGTGGACGA
    ATCACATGTT CTTCCAAAAA CAGATGCAAT GACCAGGACA CCAGAACTTC TTATAGAATT
    GGAAATTCAT GGAGTAAGAC TGACACCAGA GGAAATCTCT TGCAATGTAT CTGTACTGGC
    AATGGGCGAG GAGAGTGGAA ATGTGATAGA CATTCCTCTG CACAAGCAAC TGGTACTGGC
    TCAAACCCCA TCACAAACAT TCAAACTACC CTCTTCCAGC CTGATTCAGA GCTGGAGCCC
    TATGGTCACT GTGTGACTGA CAATGGGGTC TTGTATTCTT TGGGTATGAG ATGGCTGAAG
    GCACAGGGCA GCAAGCAGAT GCTCTGTACC TGCCTTGGTA ATGGTGTGAG CTGTGAAGAA
    ACTGTTGAAA CCATCACATT TGGTGGTAAT GCCAATGGTG AGCCATGTGC CATTCCTTTC
    ACTCATGATG GCAAGACCTA TTATTCCTGC ACAGGAGAGG GTCGTCAGGA TGGAAAATTG
    TGGTGTGCAA CAACCTCCAA CTATGACACT GACAAGAAGT ATTCATTCTG CAATGAGCAG
    CGTGCACTTG TACAGACACG TGGTGGTAAC TCCAATGGAG CTCTTTGCAA CTTTCCATTT
    TTGTACAACA ACCGCAACTA TACAGACTGC ACCTCTGAAG GTCGGAGGGA CAGTATGAAG
    TGGTGCGGGA CTACTGCCAA CTATGATGCC GATCAAAAAT TTGGATTCTG TCCCATGGCA
    GCTCATGAGG AAATATGCAC TACTAATGAG GGAGTCATGT ATAGAGTTGG TGATCAGTGG
    GACAAGCAGC ATGATCAAGG CCACATGATG CGATGTACTT GTGTTGGAAA TGGTCGTGGG
    GAATGGACTT GTGTGGCTTA TTCTCAGCTT AAAGACCAGT GCATTGTGGA TGGGATAACT
    TATGATGTGA ACAGTTCTTT TACGAAACGC CATGAGGAAG GACACATGAT GAACTGTACC
    TGCTATGGGC AGGGTCGTGG CAGATGGAAG TGCGATGCTA TAGACCAATG CCAGGATACT
    GAAACCCGAC AGTTCTATCA GATTGGAGAT TCCTGGGAAA AGCATTTGCA GGGAGTACGG
    TACCAGTGTT ACTGCTATGG CAAAGGAATT GGTGAATGGC ATTGCCAGCC TCTATCAACA
    TCCCCAGCTG GGACTGGACC CGTTCAGGTT ATTATCACAG AGAGTTCCAA TTTCCCTAAT
    TCTCACCCTA TACAGTGGAA TGCCCCACAG CCATCACACA TTAAGAATTA CATCCTGCGC
    TGGAAGCCTA AACTGAAGAC TGGCCCATGG AAACAAGCCA CTATTCCAGG ACACCTGAAC
    TCTTATACCA TCTCTGGCTT GAAACCTGGA ATCCTGTATG AGGGGCAATT GATCAGCATT
    CTGCAGTATG GAAACAGGGA GGTCACAACC TTTGACTTTA CCACCACTTC TACCATTCAC
    CGCTCCAGTC AAACTGAGAC AGGAGAAACT ACCCCACTTC CACCATTGGT GGCCACATCT
    GAGTCTGTAA CAGAAATTAC TGCTAGCAGT TTTCTGGTAT CCTGGGTCTC TGCATCTGAC
    ACAGTGTCTG GCTTCCGTGT TGAATATGAG CTGAGTGAGG ATGGAGATGA GAAACGCTAC
    CTAGAACTTC CAAACACTGC CACTTCAGTC AGTATCCCAG ACCTCCTCCC AGGCCGTAGA
    TACAATGTGA ATGTGTATCA GATAACTGAA GAGGGTGAAA AGAGCCTCAT CCTATCAACT
    ACTCAGACAA CTGCACCTGA TGCACCCCCC GACCATAATG TGGAAAATGT TGATGACACC
    TCCATCCTGA TCAAATGGAC CAAACCTCAA GCTCCCGTCA CAGGATACAG GGTTGTATAC
    AGTCCATCTG TAGAAGGCAG CAGCACAGAG TTAAACCTCC CAAGTACTGC AACTTCAGTG
    AGCCTGACTG AGCTGCTCCC AGGAATTGAA TACAATATTA CAATCTATGC AGTGGAAGAC
    AGTTTAGAAA GTGTTCCCAT CTTTATTCAG CAAGGGACAA CTGGAACTCC ACAGACAGTT
    ATAGTACCAT CTCCCACTGA CTTACAATTA GTGGAAGTTA CCGATATAAA AATTACCATC
    ATGTGGAACC CCCCACAAAG TGAGGTTTCT GGATACCGAG TTGTTGTGAA ACCTGTAAGT
    CCCTCTGACC GTGATGTGCA GAATCTCCCC GTGAGCAGGA ACACTTTTGC CGAGGTGGTT
    AACTTGCACC CTGGCAGGAC CTATACCTTT GAGGTGTATG CAGTTAATCG TGGACAAGAG
    AGTGAACCCC TGGCTGGAGA CTTCACTACC AAACTCGATG CTCCATCAGA CTTGCAGTTT
    ACAGATGTAA CTGACTCTAC AGTTGTTATT ATCTGGAGAC CACCCCAAGC CAAAATTGGT
    CGTTACCTGC TGTCTGTAGG TCAAACCCGG GGAGGCCAGC CGAGCCAATT TCACATTAAC
    CCCTCTGCCA CAAACCACAA GCTGGAGAGT CTCTCCCCAG GCACAGAGTA CACCATTTCA
    CTTGTTGCCC TCAAAGGCAA TCAGCAAAGT GCTAGCACTA CTGGTGTCTT TTCAACATTG
    GAACCTGTGG GATCAATTCC ACCTTACAAC ACTGAAGTCA CAGAGACTAC CATAGTGGTA
    ACTTGGACCC CTGTGCCCAG AATCGGGTTT AAGCTGGATG TTCGACCCAG CCAAGGTGGG
    GAGGCACCTC GGGAAGTCAT ATCCGAGTCT GGCAGCATTG TGATCTCTGG CCTAACCCCT
    GGAGTCGAAT ATATGTACAG CATATCTGTT CTTAAGGATG GCGTTGAGAG GGACATTCCC
    ATCACCAAGA CTGTTGTCAC ACCTGTCGCT CCTCCAACCA ACTTGCGCCT CCAACCAAGC
    AGAGATTCTG CTACACTTAC TGTTTACTGG GACAGTAGCA TTTCACCAGG AATCACTGGA
    TACAGAATTA CCACTGCACC CACTCCAATG CAGGTTGGCA ATTCCCTGGA AGAGGAGGTT
    GGTCCAACTC AGACATACTG CCTCTTTGAG AACCTGAGCC CTGGTGTAGA GTACAATGTC
    AGCGTGTATG CAGTAAAAGG GGAAGAGGAG AGTTCACCTC TCTCCCAGAT CTTTCTTCAA
    GAGATACCCC AACTGACTGA CCTAAAGTAT GACGATGTAA CTGACACAAG CATTGACCTG
    AGGTGGACGC CCCTTAATTC CTCCACCATT ATCGGCTACC GAATCACGGT GGTTGCGGCG
    GGAGAATCCG TCCCTATCTA CGAGGAATTT GTGGGCCCCA CAGATGGGTA TTACAAAGTT
    TCAGGATTGG AACCCGGCAT TGACTATGAG ATCAGCGTGA TAACACTTAT TAGCGGCGGA
    GAGAGCGCCC CGACCACAAT CGTACAGCAC ACGGCTGTTC CACCTCCAAC TAATTTGCGC
    TTCACCAATG TTGGCCCTGA CACCATGCGC GTCACCTGGT CACCTCCAAC TTCTATCGAA
    CTATCAAGTT TCCTGGTACG ATATTCTCCC GTGAAGAAGC CAGATGATGT GACAGAACTC
    TCCCTCTCTC CATCTACCAA CATGGTGGTT TTATCAAACT TGCTTCCCTT CACCGAATAT
    TTAGTCAGCG TTCATTCTGT TTATGAGGAG CGAGAGAGTA GTGCCCTCAA TGGTGTAGCT
    AAGACTTACT TGGACTCTCC AACTGGCATT GACTTTTCTG AAATTACTCC CAATTCATTT
    ACTGTGCACT GGATTGCTCC ACGTGCCCCT ATCACTGGCT ACAGAATTCG GTACCAGTTA
    GAAAGTGGAG CTGGGCGACC CAAGGAGGAG CGGGTACCTC CATCCCGGAA CTCTATTACC
    CTTACCCACC TCATTCCAGG TTCAGAATAC ATTGTCAGTA TCATTGCATT CAATGGTCAA
    CAGGAGAGTT TGCCACTTTC TGGACAGCAA GCCACTGTAT CTGATGTCCC AACTGATTTG
    GAAGTGACCT CCTCCTCTCC TAACACACTC ACCATCTCTT GGGAAGCACC TTCAGTGAAT
    GTACGTTACT ACAGGATCAC CCATTCCCAG ACAGGAGGTC ATGGTCCAGA GAAGGAGTTT
    ACAGTTCCAG GAACATCCAA CACTGCAACC ATCAGAGGGT TAAATCCAGG AGTAAGCTAC
    ACCATCACTG TTTATGCAGT GACTGGGCGA GGGGATAGTC CTGCCTCTAG CAAACCACTT
    ACCATTGTTC ACATGACAGA TGTTGACCAA CCAACTGACA TGGCTGTCAC AGATATCCAG
    GATCACAGCA TTCATGTCAA ATGGTCTCCT CCTCCAGGCC CAGTCACTGG TTACAGGGTC
    ACTAGTGTCC CAAAAAGTGG CCAAGGAGAA ACCTTTTCCC AGGTCGTTTC TCCAGATCAA
    ACTGAAGTGA CGATTGTGGG CCTGCAACCA GCTGTGGAGT ATGTGGTGAG CATATACTCA
    CAAGGTGAAA ATGGCGAGAG CGAGCCTCTG GTTGAAACTG CAGTTACAAA CATTGACCAT
    CCCAAAGGAC TGACATTCAC TGATGTGGGT GTTGATTCCA TTAGACTGGC TTGGGAGGTC
    CCAGATGGGC AGGTCACCCG CTACAGGGTT ACCTACTCAA GTCCTGAGGA GGGAGTAAAA
    GAGTTGTTCC CAGCTCCAGA AGGGGATGAT GACACAGCAG AATTGCACGG TCTGAGGCCG
    GGCACAGAAT ACACTGTGAG CATCGTGGCA TTGCACGATG ACATGGAGAG CAAGCCGATG
    ATTGGAATCC AGAGCACAGC AATTCCAGCA CCAACAAATC TTCAGTTCTC TCAAGTGACA
    CCAAGTGGCT TCTCTCTTAG CTGGCATGCA CCCACTATCC ATCTCAGTGG GTACCTTGTA
    CGTGTCAACC CTAAAGAGAA AACTGGGCCT ATGAAGGAAG TTAGACTTTC ACCTGGTGTG
    ACTGCTACAA CTGTAACTGG TCTGATGGTG GCCACAAAAT ATGAAGTTAA TGTGTATGCT
    CTTAAGGATA CCCTGACAAG TCAGCCACTG CAGGGTTTAA TCTCTACACT TGATAATGTA
    AGTCCACCTC GAAGACCTCG TGTTCAAGAT GTGACCGAGA CCACAGTTAC ACTGTCCTGG
    CGCACTAAAA CAGAGACCAT CACTGGCTTC CAGATTGATG CTATACCTGC CGGAGGTCAG
    AATCCCATCA GGAGAACAGT TGATGCAGAT CTCCGTTCTT TTACTATCAC AGGTCTGCAG
    CCTGGCACAG ACTACAAGAT CTATCTGTAC ACCCTAAATG ACAATGCTCG TAGCTCTCCT
    GTGATTGTTG ATGTCACCAC AGCTGTGGAC TCTCCATCCA ACCTGCGTTT CCTGACCACA
    ACTAGCAACT CCCTGCTGTT CACTTGGCAG CCACCTCGTG CTCGCATCAC TGGCTATATC
    ATTCGTTATG AGAAAGCTGG AGGGCTACTG AAAGAGCACC TCCCACGCCT CCAAGCAGGA
    ACCACAGAGT CTACCATTAC CAACCTGGAG CCAGGCACAG AATATATTGT CTATATTATT
    GCTGTGAGAA ACAATCAGAA GAGTGAGCCT CTTGTGGGAC GTAAGAGAAC AGGACCACAG
    CTAAATGATT CTGGCGTCCA AGAAGTGGCA TCCCATACTA CCATTTCATG GAGACCTGAG
    CTGGAAACCA CTGAATATAT CATCTCTTGC CATCCTATTG AACATGATGA GGCCCCATTG
    CAGTTTAGGG TCCCTGGAAC CTCCTCTAGT GCCACATTAA ATGGTCTGAC TCAAGGAGCC
    ACTTATAACA TCATTGTGGA AGCGCAGAAG GGGTCTGATA AACATAAGGT CTTCGAGAAG
    CGTGTAACTG TTGGCAGTCC TGGTTCCCCT GAGGGGGTTT TACAGCCAGC TGAGGACACC
    TGTTATGACA CATTCAATGG AGCACACTAT TCTGTGGGAC AGGAATGGGA ACGCATGTCA
    GAATCTGGAT TCAGGCTCTG GTGCAAGTGC CTGGGCTATG GAAGTGGACA CTTCAGATGT
    GACTCCTCCA AATGGTGCCA TGATAATGGT GTCAATCACA GGGTCGGGGA GAAATGGGAC
    CGACGAGGAG AGAATGGTCA GATGATGAGT TGTACTTGTC TTGGCAATGG AAAAGGAGAA
    TTTAAGTGTG AACCTCATGA AGCCACGTGC TATGATGAAG GGAAGATGTA CAATGTTGGA
    GAGCAGTGGC AGAAGGAATA TTTAGGGGCA ATCTGTTCAT GCACATGCTA TGGAGGACAG
    CAAGGGTGGA GATGTGACAA TTGCCGCAGA CCAGGTGCTG TTCTTCCCGA TGGCACTGCT
    GGGCATACAG TCTCTCAGTT TGCCCAGAGA TACCAACAGA ACTATAATTT GAACTGCCCC
    ATTGAGTGCT ACCTTCCCCT CCAAGCAGAT ATACAGCATT CGGAACAGAC CCAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa28347
    Clone ID Related Accession (Same CDS sequence) XM_012970496.2 , XM_012970496.3
    Accession Version XM_012970496.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product fibronectin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970496.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGCGCTGGG GGGCCCTGAC CGGGCTGCTC CTAGCCCTGT GCCTGTGTGC AGTGGTACGT 
    TCCGCCCCAT CAAGCAAGAA GCGCAGGCAG GCGCAGCAGC AGCAAGTAGT GCAACCCCAT
    GGATCACCGG ACAGCCAGCA GAAAGGATGT TATGACAATG GCAAGTACTA CCAAATCAAC
    CAGCAATGGG AGCGCACCTA CCTAGGGAAC ACACTGGTGT GTACTTGTTA TGGCGGAGGC
    AGAGGTTTCA ACTGCGAAAG CAAACCAGAG TCTGAAGAGA CCTGCTTTGA CAAATACACT
    GGTGTTACTT ACCGGGTCGG TGAAACATAT GAGCGACCAA AGGATAACAT GATCTGGGAC
    TGTACGTGTA TTGGAGCTGG AAGGGGACGC ATTAGCTGTA CAATTGCAAA TCGTTGCCAT
    GAAGGAGGTC AGTCTTATAA GATTGGGGAC ACCTGGCGCA GGCCACATGA AACTGGGGGG
    TATATGCTAG AATGTGTCTG CCTTGGAAAT GGTAAAGGAG AATGGACCTG CAAGCCCGTT
    GCTGAGAGAT GCTATGATAA CACAGCTGGG ATATCCTATG TAGTTGGTCA GACCTGGGAA
    AAGCCTTATC AGGGCTGGAT GATGGTGGAC TGTACATGCT TGGGAGAAGG CAGTGGACGA
    ATCACATGTT CTTCCAAAAA CAGATGCAAT GACCAGGACA CCAGAACTTC TTATAGAATT
    GGAAATTCAT GGAGTAAGAC TGACACCAGA GGAAATCTCT TGCAATGTAT CTGTACTGGC
    AATGGGCGAG GAGAGTGGAA ATGTGATAGA CATTCCTCTG CACAAGCAAC TGGTACTGGC
    TCAAACCCCA TCACAAACAT TCAAACTACC CTCTTCCAGC CTGATTCAGA GCTGGAGCCC
    TATGGTCACT GTGTGACTGA CAATGGGGTC TTGTATTCTT TGGGTATGAG ATGGCTGAAG
    GCACAGGGCA GCAAGCAGAT GCTCTGTACC TGCCTTGGTA ATGGTGTGAG CTGTGAAGAA
    ACTGTTGAAA CCATCACATT TGGTGGTAAT GCCAATGGTG AGCCATGTGC CATTCCTTTC
    ACTCATGATG GCAAGACCTA TTATTCCTGC ACAGGAGAGG GTCGTCAGGA TGGAAAATTG
    TGGTGTGCAA CAACCTCCAA CTATGACACT GACAAGAAGT ATTCATTCTG CAATGAGCAG
    CGTGCACTTG TACAGACACG TGGTGGTAAC TCCAATGGAG CTCTTTGCAA CTTTCCATTT
    TTGTACAACA ACCGCAACTA TACAGACTGC ACCTCTGAAG GTCGGAGGGA CAGTATGAAG
    TGGTGCGGGA CTACTGCCAA CTATGATGCC GATCAAAAAT TTGGATTCTG TCCCATGGCA
    GCTCATGAGG AAATATGCAC TACTAATGAG GGAGTCATGT ATAGAGTTGG TGATCAGTGG
    GACAAGCAGC ATGATCAAGG CCACATGATG CGATGTACTT GTGTTGGAAA TGGTCGTGGG
    GAATGGACTT GTGTGGCTTA TTCTCAGCTT AAAGACCAGT GCATTGTGGA TGGGATAACT
    TATGATGTGA ACAGTTCTTT TACGAAACGC CATGAGGAAG GACACATGAT GAACTGTACC
    TGCTATGGGC AGGGTCGTGG CAGATGGAAG TGCGATGCTA TAGACCAATG CCAGGATACT
    GAAACCCGAC AGTTCTATCA GATTGGAGAT TCCTGGGAAA AGCATTTGCA GGGAGTACGG
    TACCAGTGTT ACTGCTATGG CAAAGGAATT GGTGAATGGC ATTGCCAGCC TCTATCAACA
    TCCCCAGCTG GGACTGGACC CGTTCAGGTT ATTATCACAG AGAGTTCCAA TTTCCCTAAT
    TCTCACCCTA TACAGTGGAA TGCCCCACAG CCATCACACA TTAAGAATTA CATCCTGCGC
    TGGAAGCCTA AACTGAAGAC TGGCCCATGG AAACAAGCCA CTATTCCAGG ACACCTGAAC
    TCTTATACCA TCTCTGGCTT GAAACCTGGA ATCCTGTATG AGGGGCAATT GATCAGCATT
    CTGCAGTATG GAAACAGGGA GGTCACAACC TTTGACTTTA CCACCACTTC TACCATTCAC
    CGCTCCAGTC AAACTGAGAC AGGAGAAACT ACCCCACTTC CACCATTGGT GGCCACATCT
    GAGTCTGTAA CAGAAATTAC TGCTAGCAGT TTTCTGGTAT CCTGGGTCTC TGCATCTGAC
    ACAGTGTCTG GCTTCCGTGT TGAATATGAG CTGAGTGAGG ATGGAGATGA GAAACGCTAC
    CTAGAACTTC CAAACACTGC CACTTCAGTC AGTATCCCAG ACCTCCTCCC AGGCCGTAGA
    TACAATGTGA ATGTGTATCA GATAACTGAA GAGGGTGAAA AGAGCCTCAT CCTATCAACT
    ACTCAGACAA CTGCACCTGA TGCACCCCCC GACCATAATG TGGAAAATGT TGATGACACC
    TCCATCCTGA TCAAATGGAC CAAACCTCAA GCTCCCGTCA CAGGATACAG GGTTGTATAC
    AGTCCATCTG TAGAAGGCAG CAGCACAGAG TTAAACCTCC CAAGTACTGC AACTTCAGTG
    AGCCTGACTG AGCTGCTCCC AGGAATTGAA TACAATATTA CAATCTATGC AGTGGAAGAC
    AGTTTAGAAA GTGTTCCCAT CTTTATTCAG CAAGGGACAA CTGGAACTCC ACAGACAGTT
    ATAGTACCAT CTCCCACTGA CTTACAATTA GTGGAAGTTA CCGATATAAA AATTACCATC
    ATGTGGAACC CCCCACAAAG TGAGGTTTCT GGATACCGAG TTGTTGTGAA ACCTGTAAGT
    CCCTCTGACC GTGATGTGCA GAATCTCCCC GTGAGCAGGA ACACTTTTGC CGAGGTGGTT
    AACTTGCACC CTGGCAGGAC CTATACCTTT GAGGTGTATG CAGTTAATCG TGGACAAGAG
    AGTGAACCCC TGGCTGGAGA CTTCACTACC AAACTCGATG CTCCATCAGA CTTGCAGTTT
    ACAGATGTAA CTGACTCTAC AGTTGTTATT ATCTGGAGAC CACCCCAAGC CAAAATTGGT
    CGTTACCTGC TGTCTGTAGG TCAAACCCGG GGAGGCCAGC CGAGCCAATT TCACATTAAC
    CCCTCTGCCA CAAACCACAA GCTGGAGAGT CTCTCCCCAG GCACAGAGTA CACCATTTCA
    CTTGTTGCCC TCAAAGGCAA TCAGCAAAGT GCTAGCACTA CTGGTGTCTT TTCAACATTG
    GAACCTGTGG GATCAATTCC ACCTTACAAC ACTGAAGTCA CAGAGACTAC CATAGTGGTA
    ACTTGGACCC CTGTGCCCAG AATCGGGTTT AAGCTGGATG TTCGACCCAG CCAAGGTGGG
    GAGGCACCTC GGGAAGTCAT ATCCGAGTCT GGCAGCATTG TGATCTCTGG CCTAACCCCT
    GGAGTCGAAT ATATGTACAG CATATCTGTT CTTAAGGATG GCGTTGAGAG GGACATTCCC
    ATCACCAAGA CTGTTGTCAC ACCTGTCGCT CCTCCAACCA ACTTGCGCCT CCAACCAAGC
    AGAGATTCTG CTACACTTAC TGTTTACTGG GACAGTAGCA TTTCACCAGG AATCACTGGA
    TACAGAATTA CCACTGCACC CACTCCAATG CAGGTTGGCA ATTCCCTGGA AGAGGAGGTT
    GGTCCAACTC AGACATACTG CCTCTTTGAG AACCTGAGCC CTGGTGTAGA GTACAATGTC
    AGCGTGTATG CAGTAAAAGG GGAAGAGGAG AGTTCACCTC TCTCCCAGAT CTTTCTTCAA
    GCTGTTCCAC CTCCAACTAA TTTGCGCTTC ACCAATGTTG GCCCTGACAC CATGCGCGTC
    ACCTGGTCAC CTCCAACTTC TATCGAACTA TCAAGTTTCC TGGTACGATA TTCTCCCGTG
    AAGAAGCCAG ATGATGTGAC AGAACTCTCC CTCTCTCCAT CTACCAACAT GGTGGTTTTA
    TCAAACTTGC TTCCCTTCAC CGAATATTTA GTCAGCGTTC ATTCTGTTTA TGAGGAGCGA
    GAGAGTAGTG CCCTCAATGG TGTAGCTAAG ACTTACTTGG ACTCTCCAAC TGGCATTGAC
    TTTTCTGAAA TTACTCCCAA TTCATTTACT GTGCACTGGA TTGCTCCACG TGCCCCTATC
    ACTGGCTACA GAATTCGGTA CCAGTTAGAA AGTGGAGCTG GGCGACCCAA GGAGGAGCGG
    GTACCTCCAT CCCGGAACTC TATTACCCTT ACCCACCTCA TTCCAGGTTC AGAATACATT
    GTCAGTATCA TTGCATTCAA TGGTCAACAG GAGAGTTTGC CACTTTCTGG ACAGCAAGCC
    ACTGTATCTG ATGTCCCAAC TGATTTGGAA GTGACCTCCT CCTCTCCTAA CACACTCACC
    ATCTCTTGGG AAGCACCTTC AGTGAATGTA CGTTACTACA GGATCACCCA TTCCCAGACA
    GGAGGTCATG GTCCAGAGAA GGAGTTTACA GTTCCAGGAA CATCCAACAC TGCAACCATC
    AGAGGGTTAA ATCCAGGAGT AAGCTACACC ATCACTGTTT ATGCAGTGAC TGGGCGAGGG
    GATAGTCCTG CCTCTAGCAA ACCACTTACC ATTGTTCACA TGACAGATGT TGACCAACCA
    ACTGACATGG CTGTCACAGA TATCCAGGAT CACAGCATTC ATGTCAAATG GTCTCCTCCT
    CCAGGCCCAG TCACTGGTTA CAGGGTCACT AGTGTCCCAA AAAGTGGCCA AGGAGAAACC
    TTTTCCCAGG TCGTTTCTCC AGATCAAACT GAAGTGACGA TTGTGGGCCT GCAACCAGCT
    GTGGAGTATG TGGTGAGCAT ATACTCACAA GGTGAAAATG GCGAGAGCGA GCCTCTGGTT
    GAAACTGCAG TTACAACAAT TCCAGCACCA ACAAATCTTC AGTTCTCTCA AGTGACACCA
    AGTGGCTTCT CTCTTAGCTG GCATGCACCC ACTATCCATC TCAGTGGGTA CCTTGTACGT
    GTCAACCCTA AAGAGAAAAC TGGGCCTATG AAGGAAGTTA GACTTTCACC TGGTGTGACT
    GCTACAACTG TAACTGGTCT GATGGTGGCC ACAAAATATG AAGTTAATGT GTATGCTCTT
    AAGGATACCC TGACAAGTCA GCCACTGCAG GGTTTAATCT CTACACTTGA TAATGTAAGT
    CCACCTCGAA GACCTCGTGT TCAAGATGTG ACCGAGACCA CAGTTACACT GTCCTGGCGC
    ACTAAAACAG AGACCATCAC TGGCTTCCAG ATTGATGCTA TACCTGCCGG AGGTCAGAAT
    CCCATCAGGA GAACAGTTGA TGCAGATCTC CGTTCTTTTA CTATCACAGG TCTGCAGCCT
    GGCACAGACT ACAAGATCTA TCTGTACACC CTAAATGACA ATGCTCGTAG CTCTCCTGTG
    ATTGTTGATG TCACCACAGC TGTGGACTCT CCATCCAACC TGCGTTTCCT GACCACAACT
    AGCAACTCCC TGCTGTTCAC TTGGCAGCCA CCTCGTGCTC GCATCACTGG CTATATCATT
    CGTTATGAGA AAGCTGGAGG GCTACTGAAA GAGCACCTCC CACGCCTCCA AGCAGGAACC
    ACAGAGTCTA CCATTACCAA CCTGGAGCCA GGCACAGAAT ATATTGTCTA TATTATTGCT
    GTGAGAAACA ATCAGAAGAG TGAGCCTCTT GTGGGACGTA AGAGAACAGA CGAACTGCCT
    CGCCTGGTTA CACTTCCACA CCCGGGCAAA GGCCCTGAAA TCCTTGATGT TCCCACTGAT
    GAAGAGAACA CACCCCACAT CACACAAATC AAGTTGGACA ATGGTAATGG TATCCAACTG
    CCAGGCTCTA ATGGACAGCA GCCCAGTTCT GGCAATGAAG GGCAGCTTAT AGAAGAACAT
    GGTTTCAGAA GTCCCCTTGC ACCCACTACT GCTGTGCCAG TGAGGCCAGG GAAATTCACC
    CCTGGGCACT ACCCACAGGA GAGGGTTGAT ATTGAGCTAG ACACAGAGTT CCCTGTGCAG
    CGTGGAGATC TTGATGGTCC TTACCCTCAT GGCTTAGGAC CACAGCTAAA TGATTCTGGC
    GTCCAAGAAG TGGCATCCCA TACTACCATT TCATGGAGAC CTGAGCTGGA AACCACTGAA
    TATATCATCT CTTGCCATCC TATTGAACAT GATGAGGCCC CATTGCAGTT TAGGGTCCCT
    GGAACCTCCT CTAGTGCCAC ATTAAATGGT CTGACTCAAG GAGCCACTTA TAACATCATT
    GTGGAAGCGC AGAAGGGGTC TGATAAACAT AAGGTCTTCG AGAAGCGTGT AACTGTTGGC
    AGTCCTGGTT CCCCTGAGGG GGTTTTACAG CCAGCTGAGG ACACCTGTTA TGACACATTC
    AATGGAGCAC ACTATTCTGT GGGACAGGAA TGGGAACGCA TGTCAGAATC TGGATTCAGG
    CTCTGGTGCA AGTGCCTGGG CTATGGAAGT GGACACTTCA GATGTGACTC CTCCAAATGG
    TGCCATGATA ATGGTGTCAA TCACAGGGTC GGGGAGAAAT GGGACCGACG AGGAGAGAAT
    GGTCAGATGA TGAGTTGTAC TTGTCTTGGC AATGGAAAAG GAGAATTTAA GTGTGAACCT
    CATGAAGCCA CGTGCTATGA TGAAGGGAAG ATGTACAATG TTGGAGAGCA GTGGCAGAAG
    GAATATTTAG GGGCAATCTG TTCATGCACA TGCTATGGAG GACAGCAAGG GTGGAGATGT
    GACAATTGCC GCAGACCAGG TGCTGTTCTT CCCGATGGCA CTGCTGGGCA TACAGTCTCT
    CAGTTTGCCC AGAGATACCA ACAGAACTAT AATTTGAACT GCCCCATTGA GTGCTACCTT
    CCCCTCCAAG CAGATATACA GCATTCGGAA CAGACCCAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825950.1
    CDS222..7106
    Translation

    Target ORF information:

    RefSeq Version XM_012970496.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis fibronectin 1 (fn1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970496.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGCGCTGGG GGGCCCTGAC CGGGCTGCTC CTAGCCCTGT GCCTGTGTGC AGTGGTACGT 
    TCCGCCCCAT CAAGCAAGAA GCGCAGGCAG GCGCAGCAGC AGCAAGTAGT GCAACCCCAT
    GGATCACCGG ACAGCCAGCA GAAAGGATGT TATGACAATG GCAAGTACTA CCAAATCAAC
    CAGCAATGGG AGCGCACCTA CCTAGGGAAC ACACTGGTGT GTACTTGTTA TGGCGGAGGC
    AGAGGTTTCA ACTGCGAAAG CAAACCAGAG TCTGAAGAGA CCTGCTTTGA CAAATACACT
    GGTGTTACTT ACCGGGTCGG TGAAACATAT GAGCGACCAA AGGATAACAT GATCTGGGAC
    TGTACGTGTA TTGGAGCTGG AAGGGGACGC ATTAGCTGTA CAATTGCAAA TCGTTGCCAT
    GAAGGAGGTC AGTCTTATAA GATTGGGGAC ACCTGGCGCA GGCCACATGA AACTGGGGGG
    TATATGCTAG AATGTGTCTG CCTTGGAAAT GGTAAAGGAG AATGGACCTG CAAGCCCGTT
    GCTGAGAGAT GCTATGATAA CACAGCTGGG ATATCCTATG TAGTTGGTCA GACCTGGGAA
    AAGCCTTATC AGGGCTGGAT GATGGTGGAC TGTACATGCT TGGGAGAAGG CAGTGGACGA
    ATCACATGTT CTTCCAAAAA CAGATGCAAT GACCAGGACA CCAGAACTTC TTATAGAATT
    GGAAATTCAT GGAGTAAGAC TGACACCAGA GGAAATCTCT TGCAATGTAT CTGTACTGGC
    AATGGGCGAG GAGAGTGGAA ATGTGATAGA CATTCCTCTG CACAAGCAAC TGGTACTGGC
    TCAAACCCCA TCACAAACAT TCAAACTACC CTCTTCCAGC CTGATTCAGA GCTGGAGCCC
    TATGGTCACT GTGTGACTGA CAATGGGGTC TTGTATTCTT TGGGTATGAG ATGGCTGAAG
    GCACAGGGCA GCAAGCAGAT GCTCTGTACC TGCCTTGGTA ATGGTGTGAG CTGTGAAGAA
    ACTGTTGAAA CCATCACATT TGGTGGTAAT GCCAATGGTG AGCCATGTGC CATTCCTTTC
    ACTCATGATG GCAAGACCTA TTATTCCTGC ACAGGAGAGG GTCGTCAGGA TGGAAAATTG
    TGGTGTGCAA CAACCTCCAA CTATGACACT GACAAGAAGT ATTCATTCTG CAATGAGCAG
    CGTGCACTTG TACAGACACG TGGTGGTAAC TCCAATGGAG CTCTTTGCAA CTTTCCATTT
    TTGTACAACA ACCGCAACTA TACAGACTGC ACCTCTGAAG GTCGGAGGGA CAGTATGAAG
    TGGTGCGGGA CTACTGCCAA CTATGATGCC GATCAAAAAT TTGGATTCTG TCCCATGGCA
    GCTCATGAGG AAATATGCAC TACTAATGAG GGAGTCATGT ATAGAGTTGG TGATCAGTGG
    GACAAGCAGC ATGATCAAGG CCACATGATG CGATGTACTT GTGTTGGAAA TGGTCGTGGG
    GAATGGACTT GTGTGGCTTA TTCTCAGCTT AAAGACCAGT GCATTGTGGA TGGGATAACT
    TATGATGTGA ACAGTTCTTT TACGAAACGC CATGAGGAAG GACACATGAT GAACTGTACC
    TGCTATGGGC AGGGTCGTGG CAGATGGAAG TGCGATGCTA TAGACCAATG CCAGGATACT
    GAAACCCGAC AGTTCTATCA GATTGGAGAT TCCTGGGAAA AGCATTTGCA GGGAGTACGG
    TACCAGTGTT ACTGCTATGG CAAAGGAATT GGTGAATGGC ATTGCCAGCC TCTATCAACA
    TCCCCAGCTG GGACTGGACC CGTTCAGGTT ATTATCACAG AGAGTTCCAA TTTCCCTAAT
    TCTCACCCTA TACAGTGGAA TGCCCCACAG CCATCACACA TTAAGAATTA CATCCTGCGC
    TGGAAGCCTA AACTGAAGAC TGGCCCATGG AAACAAGCCA CTATTCCAGG ACACCTGAAC
    TCTTATACCA TCTCTGGCTT GAAACCTGGA ATCCTGTATG AGGGGCAATT GATCAGCATT
    CTGCAGTATG GAAACAGGGA GGTCACAACC TTTGACTTTA CCACCACTTC TACCATTCAC
    CGCTCCAGTC AAACTGAGAC AGGAGAAACT ACCCCACTTC CACCATTGGT GGCCACATCT
    GAGTCTGTAA CAGAAATTAC TGCTAGCAGT TTTCTGGTAT CCTGGGTCTC TGCATCTGAC
    ACAGTGTCTG GCTTCCGTGT TGAATATGAG CTGAGTGAGG ATGGAGATGA GAAACGCTAC
    CTAGAACTTC CAAACACTGC CACTTCAGTC AGTATCCCAG ACCTCCTCCC AGGCCGTAGA
    TACAATGTGA ATGTGTATCA GATAACTGAA GAGGGTGAAA AGAGCCTCAT CCTATCAACT
    ACTCAGACAA CTGCACCTGA TGCACCCCCC GACCATAATG TGGAAAATGT TGATGACACC
    TCCATCCTGA TCAAATGGAC CAAACCTCAA GCTCCCGTCA CAGGATACAG GGTTGTATAC
    AGTCCATCTG TAGAAGGCAG CAGCACAGAG TTAAACCTCC CAAGTACTGC AACTTCAGTG
    AGCCTGACTG AGCTGCTCCC AGGAATTGAA TACAATATTA CAATCTATGC AGTGGAAGAC
    AGTTTAGAAA GTGTTCCCAT CTTTATTCAG CAAGGGACAA CTGGAACTCC ACAGACAGTT
    ATAGTACCAT CTCCCACTGA CTTACAATTA GTGGAAGTTA CCGATATAAA AATTACCATC
    ATGTGGAACC CCCCACAAAG TGAGGTTTCT GGATACCGAG TTGTTGTGAA ACCTGTAAGT
    CCCTCTGACC GTGATGTGCA GAATCTCCCC GTGAGCAGGA ACACTTTTGC CGAGGTGGTT
    AACTTGCACC CTGGCAGGAC CTATACCTTT GAGGTGTATG CAGTTAATCG TGGACAAGAG
    AGTGAACCCC TGGCTGGAGA CTTCACTACC AAACTCGATG CTCCATCAGA CTTGCAGTTT
    ACAGATGTAA CTGACTCTAC AGTTGTTATT ATCTGGAGAC CACCCCAAGC CAAAATTGGT
    CGTTACCTGC TGTCTGTAGG TCAAACCCGG GGAGGCCAGC CGAGCCAATT TCACATTAAC
    CCCTCTGCCA CAAACCACAA GCTGGAGAGT CTCTCCCCAG GCACAGAGTA CACCATTTCA
    CTTGTTGCCC TCAAAGGCAA TCAGCAAAGT GCTAGCACTA CTGGTGTCTT TTCAACATTG
    GAACCTGTGG GATCAATTCC ACCTTACAAC ACTGAAGTCA CAGAGACTAC CATAGTGGTA
    ACTTGGACCC CTGTGCCCAG AATCGGGTTT AAGCTGGATG TTCGACCCAG CCAAGGTGGG
    GAGGCACCTC GGGAAGTCAT ATCCGAGTCT GGCAGCATTG TGATCTCTGG CCTAACCCCT
    GGAGTCGAAT ATATGTACAG CATATCTGTT CTTAAGGATG GCGTTGAGAG GGACATTCCC
    ATCACCAAGA CTGTTGTCAC ACCTGTCGCT CCTCCAACCA ACTTGCGCCT CCAACCAAGC
    AGAGATTCTG CTACACTTAC TGTTTACTGG GACAGTAGCA TTTCACCAGG AATCACTGGA
    TACAGAATTA CCACTGCACC CACTCCAATG CAGGTTGGCA ATTCCCTGGA AGAGGAGGTT
    GGTCCAACTC AGACATACTG CCTCTTTGAG AACCTGAGCC CTGGTGTAGA GTACAATGTC
    AGCGTGTATG CAGTAAAAGG GGAAGAGGAG AGTTCACCTC TCTCCCAGAT CTTTCTTCAA
    GCTGTTCCAC CTCCAACTAA TTTGCGCTTC ACCAATGTTG GCCCTGACAC CATGCGCGTC
    ACCTGGTCAC CTCCAACTTC TATCGAACTA TCAAGTTTCC TGGTACGATA TTCTCCCGTG
    AAGAAGCCAG ATGATGTGAC AGAACTCTCC CTCTCTCCAT CTACCAACAT GGTGGTTTTA
    TCAAACTTGC TTCCCTTCAC CGAATATTTA GTCAGCGTTC ATTCTGTTTA TGAGGAGCGA
    GAGAGTAGTG CCCTCAATGG TGTAGCTAAG ACTTACTTGG ACTCTCCAAC TGGCATTGAC
    TTTTCTGAAA TTACTCCCAA TTCATTTACT GTGCACTGGA TTGCTCCACG TGCCCCTATC
    ACTGGCTACA GAATTCGGTA CCAGTTAGAA AGTGGAGCTG GGCGACCCAA GGAGGAGCGG
    GTACCTCCAT CCCGGAACTC TATTACCCTT ACCCACCTCA TTCCAGGTTC AGAATACATT
    GTCAGTATCA TTGCATTCAA TGGTCAACAG GAGAGTTTGC CACTTTCTGG ACAGCAAGCC
    ACTGTATCTG ATGTCCCAAC TGATTTGGAA GTGACCTCCT CCTCTCCTAA CACACTCACC
    ATCTCTTGGG AAGCACCTTC AGTGAATGTA CGTTACTACA GGATCACCCA TTCCCAGACA
    GGAGGTCATG GTCCAGAGAA GGAGTTTACA GTTCCAGGAA CATCCAACAC TGCAACCATC
    AGAGGGTTAA ATCCAGGAGT AAGCTACACC ATCACTGTTT ATGCAGTGAC TGGGCGAGGG
    GATAGTCCTG CCTCTAGCAA ACCACTTACC ATTGTTCACA TGACAGATGT TGACCAACCA
    ACTGACATGG CTGTCACAGA TATCCAGGAT CACAGCATTC ATGTCAAATG GTCTCCTCCT
    CCAGGCCCAG TCACTGGTTA CAGGGTCACT AGTGTCCCAA AAAGTGGCCA AGGAGAAACC
    TTTTCCCAGG TCGTTTCTCC AGATCAAACT GAAGTGACGA TTGTGGGCCT GCAACCAGCT
    GTGGAGTATG TGGTGAGCAT ATACTCACAA GGTGAAAATG GCGAGAGCGA GCCTCTGGTT
    GAAACTGCAG TTACAACAAT TCCAGCACCA ACAAATCTTC AGTTCTCTCA AGTGACACCA
    AGTGGCTTCT CTCTTAGCTG GCATGCACCC ACTATCCATC TCAGTGGGTA CCTTGTACGT
    GTCAACCCTA AAGAGAAAAC TGGGCCTATG AAGGAAGTTA GACTTTCACC TGGTGTGACT
    GCTACAACTG TAACTGGTCT GATGGTGGCC ACAAAATATG AAGTTAATGT GTATGCTCTT
    AAGGATACCC TGACAAGTCA GCCACTGCAG GGTTTAATCT CTACACTTGA TAATGTAAGT
    CCACCTCGAA GACCTCGTGT TCAAGATGTG ACCGAGACCA CAGTTACACT GTCCTGGCGC
    ACTAAAACAG AGACCATCAC TGGCTTCCAG ATTGATGCTA TACCTGCCGG AGGTCAGAAT
    CCCATCAGGA GAACAGTTGA TGCAGATCTC CGTTCTTTTA CTATCACAGG TCTGCAGCCT
    GGCACAGACT ACAAGATCTA TCTGTACACC CTAAATGACA ATGCTCGTAG CTCTCCTGTG
    ATTGTTGATG TCACCACAGC TGTGGACTCT CCATCCAACC TGCGTTTCCT GACCACAACT
    AGCAACTCCC TGCTGTTCAC TTGGCAGCCA CCTCGTGCTC GCATCACTGG CTATATCATT
    CGTTATGAGA AAGCTGGAGG GCTACTGAAA GAGCACCTCC CACGCCTCCA AGCAGGAACC
    ACAGAGTCTA CCATTACCAA CCTGGAGCCA GGCACAGAAT ATATTGTCTA TATTATTGCT
    GTGAGAAACA ATCAGAAGAG TGAGCCTCTT GTGGGACGTA AGAGAACAGA CGAACTGCCT
    CGCCTGGTTA CACTTCCACA CCCGGGCAAA GGCCCTGAAA TCCTTGATGT TCCCACTGAT
    GAAGAGAACA CACCCCACAT CACACAAATC AAGTTGGACA ATGGTAATGG TATCCAACTG
    CCAGGCTCTA ATGGACAGCA GCCCAGTTCT GGCAATGAAG GGCAGCTTAT AGAAGAACAT
    GGTTTCAGAA GTCCCCTTGC ACCCACTACT GCTGTGCCAG TGAGGCCAGG GAAATTCACC
    CCTGGGCACT ACCCACAGGA GAGGGTTGAT ATTGAGCTAG ACACAGAGTT CCCTGTGCAG
    CGTGGAGATC TTGATGGTCC TTACCCTCAT GGCTTAGGAC CACAGCTAAA TGATTCTGGC
    GTCCAAGAAG TGGCATCCCA TACTACCATT TCATGGAGAC CTGAGCTGGA AACCACTGAA
    TATATCATCT CTTGCCATCC TATTGAACAT GATGAGGCCC CATTGCAGTT TAGGGTCCCT
    GGAACCTCCT CTAGTGCCAC ATTAAATGGT CTGACTCAAG GAGCCACTTA TAACATCATT
    GTGGAAGCGC AGAAGGGGTC TGATAAACAT AAGGTCTTCG AGAAGCGTGT AACTGTTGGC
    AGTCCTGGTT CCCCTGAGGG GGTTTTACAG CCAGCTGAGG ACACCTGTTA TGACACATTC
    AATGGAGCAC ACTATTCTGT GGGACAGGAA TGGGAACGCA TGTCAGAATC TGGATTCAGG
    CTCTGGTGCA AGTGCCTGGG CTATGGAAGT GGACACTTCA GATGTGACTC CTCCAAATGG
    TGCCATGATA ATGGTGTCAA TCACAGGGTC GGGGAGAAAT GGGACCGACG AGGAGAGAAT
    GGTCAGATGA TGAGTTGTAC TTGTCTTGGC AATGGAAAAG GAGAATTTAA GTGTGAACCT
    CATGAAGCCA CGTGCTATGA TGAAGGGAAG ATGTACAATG TTGGAGAGCA GTGGCAGAAG
    GAATATTTAG GGGCAATCTG TTCATGCACA TGCTATGGAG GACAGCAAGG GTGGAGATGT
    GACAATTGCC GCAGACCAGG TGCTGTTCTT CCCGATGGCA CTGCTGGGCA TACAGTCTCT
    CAGTTTGCCC AGAGATACCA ACAGAACTAT AATTTGAACT GCCCCATTGA GTGCTACCTT
    CCCCTCCAAG CAGATATACA GCATTCGGAA CAGACCCAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46596
    Clone ID Related Accession (Same CDS sequence) XM_018097141.1
    Accession Version XM_018097141.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7161bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product fibronectin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCGCTGGG GGGCCCTGAC CGGGCTGCTC CTAGCCCTGT GCCTGTGTGC AGTGGTACGT 
    TCCGCCCCAT CAAGCAAGAA GCGCAGGCAG GCGCAGCAGC AGCAAGTAGT GCAACCCCAT
    GGATCACCGG ACAGCCAGCA GAAAGGATGT TATGACAATG GCAAGTACTA CCAAATCAAC
    CAGCAATGGG AGCGCACCTA CCTAGGGAAC ACACTGGTGT GTACTTGTTA TGGCGGAGGC
    AGAGGTTTCA ACTGCGAAAG CAAACCAGAG TCTGAAGAGA CCTGCTTTGA CAAATACACT
    GGTGTTACTT ACCGGGTCGG TGAAACATAT GAGCGACCAA AGGATAACAT GATCTGGGAC
    TGTACGTGTA TTGGAGCTGG AAGGGGACGC ATTAGCTGTA CAATTGCAAA TCGTTGCCAT
    GAAGGAGGTC AGTCTTATAA GATTGGGGAC ACCTGGCGCA GGCCACATGA AACTGGGGGG
    TATATGCTAG AATGTGTCTG CCTTGGAAAT GGTAAAGGAG AATGGACCTG CAAGCCCGTT
    GCTGAGAGAT GCTATGATAA CACAGCTGGG ATATCCTATG TAGTTGGTCA GACCTGGGAA
    AAGCCTTATC AGGGCTGGAT GATGGTGGAC TGTACATGCT TGGGAGAAGG CAGTGGACGA
    ATCACATGTT CTTCCAAAAA CAGATGCAAT GACCAGGACA CCAGAACTTC TTATAGAATT
    GGAAATTCAT GGAGTAAGAC TGACACCAGA GGAAATCTCT TGCAATGTAT CTGTACTGGC
    AATGGGCGAG GAGAGTGGAA ATGTGATAGA CATTCCTCTG CACAAGCAAC TGGTACTGGC
    TCAAACCCCA TCACAAACAT TCAAACTACC CTCTTCCAGC CTGATTCAGA GCTGGAGCCC
    TATGGTCACT GTGTGACTGA CAATGGGGTC TTGTATTCTT TGGGTATGAG ATGGCTGAAG
    GCACAGGGCA GCAAGCAGAT GCTCTGTACC TGCCTTGGTA ATGGTGTGAG CTGTGAAGAA
    ACTGTTGAAA CCATCACATT TGGTGGTAAT GCCAATGGTG AGCCATGTGC CATTCCTTTC
    ACTCATGATG GCAAGACCTA TTATTCCTGC ACAGGAGAGG GTCGTCAGGA TGGAAAATTG
    TGGTGTGCAA CAACCTCCAA CTATGACACT GACAAGAAGT ATTCATTCTG CAATGAGCAG
    CGTGCACTTG TACAGACACG TGGTGGTAAC TCCAATGGAG CTCTTTGCAA CTTTCCATTT
    TTGTACAACA ACCGCAACTA TACAGACTGC ACCTCTGAAG GTCGGAGGGA CAGTATGAAG
    TGGTGCGGGA CTACTGCCAA CTATGATGCC GATCAAAAAT TTGGATTCTG TCCCATGGCA
    GCTCATGAGG AAATATGCAC TACTAATGAG GGAGTCATGT ATAGAGTTGG TGATCAGTGG
    GACAAGCAGC ATGATCAAGG CCACATGATG CGATGTACTT GTGTTGGAAA TGGTCGTGGG
    GAATGGACTT GTGTGGCTTA TTCTCAGCTT AAAGACCAGT GCATTGTGGA TGGGATAACT
    TATGATGTGA ACAGTTCTTT TACGAAACGC CATGAGGAAG GACACATGAT GAACTGTACC
    TGCTATGGGC AGGGTCGTGG CAGATGGAAG TGCGATGCTA TAGACCAATG CCAGGATACT
    GAAACCCGAC AGTTCTATCA GATTGGAGAT TCCTGGGAAA AGCATTTGCA GGGAGTACGG
    TACCAGTGTT ACTGCTATGG CAAAGGAATT GGTGAATGGC ATTGCCAGCC TCTATCAACA
    TCCCCAGCTG GGACTGGACC CGTTCAGGTT ATTATCACAG AGAGTTCCAA TTTCCCTAAT
    TCTCACCCTA TACAGTGGAA TGCCCCACAG CCATCACACA TTAAGAATTA CATCCTGCGC
    TGGAAGCCTA AACTGAAGAC TGGCCCATGG AAACAAGCCA CTATTCCAGG ACACCTGAAC
    TCTTATACCA TCTCTGGCTT GAAACCTGGA ATCCTGTATG AGGGGCAATT GATCAGCATT
    CTGCAGTATG GAAACAGGGA GGTCACAACC TTTGACTTTA CCACCACTTC TACCATTCAC
    CGCTCCAGTC AAACTGAGAC AGGAGAAACT ACCCCACTTC CACCATTGGT GGCCACATCT
    GAGTCTGTAA CAGAAATTAC TGCTAGCAGT TTTCTGGTAT CCTGGGTCTC TGCATCTGAC
    ACAGTGTCTG GCTTCCGTGT TGAATATGAG CTGAGTGAGG ATGGAGATGA GAAACGCTAC
    CTAGAACTTC CAAACACTGC CACTTCAGTC AGTATCCCAG ACCTCCTCCC AGGCCGTAGA
    TACAATGTGA ATGTGTATCA GATAACTGAA GAGGGTGAAA AGAGCCTCAT CCTATCAACT
    ACTCAGACAA CTGCACCTGA TGCACCCCCC GACCATAATG TGGAAAATGT TGATGACACC
    TCCATCCTGA TCAAATGGAC CAAACCTCAA GCTCCCGTCA CAGGATACAG GGTTGTATAC
    AGTCCATCTG TAGAAGGCAG CAGCACAGAG TTAAACCTCC CAAGTACTGC AACTTCAGTG
    AGCCTGACTG AGCTGCTCCC AGGAATTGAA TACAATATTA CAATCTATGC AGTGGAAGAC
    AGTTTAGAAA GTGTTCCCAT CTTTATTCAG CAAGGGACAA CTGGAACTCC ACAGACAGTT
    ATAGTACCAT CTCCCACTGA CTTACAATTA GTGGAAGTTA CCGATATAAA AATTACCATC
    ATGTGGAACC CCCCACAAAG TGAGGTTTCT GGATACCGAG TTGTTGTGAA ACCTGTAAGT
    CCCTCTGACC GTGATGTGCA GAATCTCCCC GTGAGCAGGA ACACTTTTGC CGAGGTGGTT
    AACTTGCACC CTGGCAGGAC CTATACCTTT GAGGTGTATG CAGTTAATCG TGGACAAGAG
    AGTGAACCCC TGGCTGGAGA CTTCACTACC ATGGAACCTG TGGGATCAAT TCCACCTTAC
    AACACTGAAG TCACAGAGAC TACCATAGTG GTAACTTGGA CCCCTGTGCC CAGAATCGGG
    TTTAAGCTGG ATGTTCGACC CAGCCAAGGT GGGGAGGCAC CTCGGGAAGT CATATCCGAG
    TCTGGCAGCA TTGTGATCTC TGGCCTAACC CCTGGAGTCG AATATATGTA CAGCATATCT
    GTTCTTAAGG ATGGCGTTGA GAGGGACATT CCCATCACCA AGACTGTTGT CACACCTGTC
    GCTCCTCCAA CCAACTTGCG CCTCCAACCA AGCAGAGATT CTGCTACACT TACTGTTTAC
    TGGGACAGTA GCATTTCACC AGGAATCACT GGATACAGAA TTACCACTGC ACCCACTCCA
    ATGCAGGTTG GCAATTCCCT GGAAGAGGAG GTTGGTCCAA CTCAGACATA CTGCCTCTTT
    GAGAACCTGA GCCCTGGTGT AGAGTACAAT GTCAGCGTGT ATGCAGTAAA AGGGGAAGAG
    GAGAGTTCAC CTCTCTCCCA GATCTTTCTT CAAGAGATAC CCCAACTGAC TGACCTAAAG
    TATGACGATG TAACTGACAC AAGCATTGAC CTGAGGTGGA CGCCCCTTAA TTCCTCCACC
    ATTATCGGCT ACCGAATCAC GGTGGTTGCG GCGGGAGAAT CCGTCCCTAT CTACGAGGAA
    TTTGTGGGCC CCACAGATGG GTATTACAAA GTTTCAGGAT TGGAACCCGG CATTGACTAT
    GAGATCAGCG TGATAACACT TATTAGCGGC GGAGAGAGCG CCCCGACCAC AATCGTACAG
    CACACGGCTG TTCCACCTCC AACTAATTTG CGCTTCACCA ATGTTGGCCC TGACACCATG
    CGCGTCACCT GGTCACCTCC AACTTCTATC GAACTATCAA GTTTCCTGGT ACGATATTCT
    CCCGTGAAGA AGCCAGATGA TGTGACAGAA CTCTCCCTCT CTCCATCTAC CAACATGGTG
    GTTTTATCAA ACTTGCTTCC CTTCACCGAA TATTTAGTCA GCGTTCATTC TGTTTATGAG
    GAGCGAGAGA GTAGTGCCCT CAATGGTGTA GCTAAGACTT ACTTGGACTC TCCAACTGGC
    ATTGACTTTT CTGAAATTAC TCCCAATTCA TTTACTGTGC ACTGGATTGC TCCACGTGCC
    CCTATCACTG GCTACAGAAT TCGGTACCAG TTAGAAAGTG GAGCTGGGCG ACCCAAGGAG
    GAGCGGGTAC CTCCATCCCG GAACTCTATT ACCCTTACCC ACCTCATTCC AGGTTCAGAA
    TACATTGTCA GTATCATTGC ATTCAATGGT CAACAGGAGA GTTTGCCACT TTCTGGACAG
    CAAGCCACTG TATCTGATGT CCCAACTGAT TTGGAAGTGA CCTCCTCCTC TCCTAACACA
    CTCACCATCT CTTGGGAAGC ACCTTCAGTG AATGTACGTT ACTACAGGAT CACCCATTCC
    CAGACAGGAG GTCATGGTCC AGAGAAGGAG TTTACAGTTC CAGGAACATC CAACACTGCA
    ACCATCAGAG GGTTAAATCC AGGAGTAAGC TACACCATCA CTGTTTATGC AGTGACTGGG
    CGAGGGGATA GTCCTGCCTC TAGCAAACCA CTTACCATTG TTCACATGAC AGATGTTGAC
    CAACCAACTG ACATGGCTGT CACAGATATC CAGGATCACA GCATTCATGT CAAATGGTCT
    CCTCCTCCAG GCCCAGTCAC TGGTTACAGG GTCACTAGTG TCCCAAAAAG TGGCCAAGGA
    GAAACCTTTT CCCAGGTCGT TTCTCCAGAT CAAACTGAAG TGACGATTGT GGGCCTGCAA
    CCAGCTGTGG AGTATGTGGT GAGCATATAC TCACAAGGTG AAAATGGCGA GAGCGAGCCT
    CTGGTTGAAA CTGCAGTTAC AAACATTGAC CATCCCAAAG GACTGACATT CACTGATGTG
    GGTGTTGATT CCATTAGACT GGCTTGGGAG GTCCCAGATG GGCAGGTCAC CCGCTACAGG
    GTTACCTACT CAAGTCCTGA GGAGGGAGTA AAAGAGTTGT TCCCAGCTCC AGAAGGGGAT
    GATGACACAG CAGAATTGCA CGGTCTGAGG CCGGGCACAG AATACACTGT GAGCATCGTG
    GCATTGCACG ATGACATGGA GAGCAAGCCG ATGATTGGAA TCCAGAGCAC AGCAATTCCA
    GCACCAACAA ATCTTCAGTT CTCTCAAGTG ACACCAAGTG GCTTCTCTCT TAGCTGGCAT
    GCACCCACTA TCCATCTCAG TGGGTACCTT GTACGTGTCA ACCCTAAAGA GAAAACTGGG
    CCTATGAAGG AAGTTAGACT TTCACCTGGT GTGACTGCTA CAACTGTAAC TGGTCTGATG
    GTGGCCACAA AATATGAAGT TAATGTGTAT GCTCTTAAGG ATACCCTGAC AAGTCAGCCA
    CTGCAGGGTT TAATCTCTAC ACTTGATAAT GTAAGTCCAC CTCGAAGACC TCGTGTTCAA
    GATGTGACCG AGACCACAGT TACACTGTCC TGGCGCACTA AAACAGAGAC CATCACTGGC
    TTCCAGATTG ATGCTATACC TGCCGGAGGT CAGAATCCCA TCAGGAGAAC AGTTGATGCA
    GATCTCCGTT CTTTTACTAT CACAGGTCTG CAGCCTGGCA CAGACTACAA GATCTATCTG
    TACACCCTAA ATGACAATGC TCGTAGCTCT CCTGTGATTG TTGATGTCAC CACAGCTGTG
    GACTCTCCAT CCAACCTGCG TTTCCTGACC ACAACTAGCA ACTCCCTGCT GTTCACTTGG
    CAGCCACCTC GTGCTCGCAT CACTGGCTAT ATCATTCGTT ATGAGAAAGC TGGAGGGCTA
    CTGAAAGAGC ACCTCCCACG CCTCCAAGCA GGAACCACAG AGTCTACCAT TACCAACCTG
    GAGCCAGGCA CAGAATATAT TGTCTATATT ATTGCTGTGA GAAACAATCA GAAGAGTGAG
    CCTCTTGTGG GACGTAAGAG AACAGACGAA CTGCCTCGCC TGGTTACACT TCCACACCCG
    GGCAAAGGCC CTGAAATCCT TGATGTTCCC ACTGATGAAG AGAACACACC CCACATCACA
    CAAATCAAGT TGGACAATGG TAATGGTATC CAACTGCCAG GCTCTAATGG ACAGCAGCCC
    AGTTCTGGCA ATGAAGGGCA GCTTATAGAA GAACATGGTT TCAGAAGTCC CCTTGCACCC
    ACTACTGCTG TGCCAGTGAG GCCAGGGAAA TTCACCCCTG GGCACTACCC ACAGGAGAGG
    GTTGATATTG AGCTAGACAC AGAGTTCCCT GTGCAGCGTG GAGATCTTGA TGGTCCTTAC
    CCTCATGGCT TAGGACCACA GCTAAATGAT TCTGGCGTCC AAGAAGTGGC ATCCCATACT
    ACCATTTCAT GGAGACCTGA GCTGGAAACC ACTGAATATA TCATCTCTTG CCATCCTATT
    GAACATGATG AGGCCCCATT GCAGTTTAGG GTCCCTGGAA CCTCCTCTAG TGCCACATTA
    AATGGTCTGA CTCAAGGAGC CACTTATAAC ATCATTGTGG AAGCGCAGAA GGGGTCTGAT
    AAACATAAGG TCTTCGAGAA GCGTGTAACT GTTGGCAGTC CTGGTTCCCC TGAGGGGGTT
    TTACAGCCAG CTGAGGACAC CTGTTATGAC ACATTCAATG GAGCACACTA TTCTGTGGGA
    CAGGAATGGG AACGCATGTC AGAATCTGGA TTCAGGCTCT GGTGCAAGTG CCTGGGCTAT
    GGAAGTGGAC ACTTCAGATG TGACTCCTCC AAATGGTGCC ATGATAATGG TGTCAATCAC
    AGGGTCGGGG AGAAATGGGA CCGACGAGGA GAGAATGGTC AGATGATGAG TTGTACTTGT
    CTTGGCAATG GAAAAGGAGA ATTTAAGTGT GAACCTCATG AAGCCACGTG CTATGATGAA
    GGGAAGATGT ACAATGTTGG AGAGCAGTGG CAGAAGGAAT ATTTAGGGGC AATCTGTTCA
    TGCACATGCT ATGGAGGACA GCAAGGGTGG AGATGTGACA ATTGCCGCAG ACCAGGTGCT
    GTTCTTCCCG ATGGCACTGC TGGGCATACA GTCTCTCAGT TTGCCCAGAG ATACCAACAG
    AACTATAATT TGAACTGCCC CATTGAGTGC TACCTTCCCC TCCAAGCAGA TATACAGCAT
    TCGGAACAGA CCCAGAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952630.1
    CDS222..7382
    Translation

    Target ORF information:

    RefSeq Version XM_018097141.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis fibronectin 1 (fn1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018097141.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCGCTGGG GGGCCCTGAC CGGGCTGCTC CTAGCCCTGT GCCTGTGTGC AGTGGTACGT 
    TCCGCCCCAT CAAGCAAGAA GCGCAGGCAG GCGCAGCAGC AGCAAGTAGT GCAACCCCAT
    GGATCACCGG ACAGCCAGCA GAAAGGATGT TATGACAATG GCAAGTACTA CCAAATCAAC
    CAGCAATGGG AGCGCACCTA CCTAGGGAAC ACACTGGTGT GTACTTGTTA TGGCGGAGGC
    AGAGGTTTCA ACTGCGAAAG CAAACCAGAG TCTGAAGAGA CCTGCTTTGA CAAATACACT
    GGTGTTACTT ACCGGGTCGG TGAAACATAT GAGCGACCAA AGGATAACAT GATCTGGGAC
    TGTACGTGTA TTGGAGCTGG AAGGGGACGC ATTAGCTGTA CAATTGCAAA TCGTTGCCAT
    GAAGGAGGTC AGTCTTATAA GATTGGGGAC ACCTGGCGCA GGCCACATGA AACTGGGGGG
    TATATGCTAG AATGTGTCTG CCTTGGAAAT GGTAAAGGAG AATGGACCTG CAAGCCCGTT
    GCTGAGAGAT GCTATGATAA CACAGCTGGG ATATCCTATG TAGTTGGTCA GACCTGGGAA
    AAGCCTTATC AGGGCTGGAT GATGGTGGAC TGTACATGCT TGGGAGAAGG CAGTGGACGA
    ATCACATGTT CTTCCAAAAA CAGATGCAAT GACCAGGACA CCAGAACTTC TTATAGAATT
    GGAAATTCAT GGAGTAAGAC TGACACCAGA GGAAATCTCT TGCAATGTAT CTGTACTGGC
    AATGGGCGAG GAGAGTGGAA ATGTGATAGA CATTCCTCTG CACAAGCAAC TGGTACTGGC
    TCAAACCCCA TCACAAACAT TCAAACTACC CTCTTCCAGC CTGATTCAGA GCTGGAGCCC
    TATGGTCACT GTGTGACTGA CAATGGGGTC TTGTATTCTT TGGGTATGAG ATGGCTGAAG
    GCACAGGGCA GCAAGCAGAT GCTCTGTACC TGCCTTGGTA ATGGTGTGAG CTGTGAAGAA
    ACTGTTGAAA CCATCACATT TGGTGGTAAT GCCAATGGTG AGCCATGTGC CATTCCTTTC
    ACTCATGATG GCAAGACCTA TTATTCCTGC ACAGGAGAGG GTCGTCAGGA TGGAAAATTG
    TGGTGTGCAA CAACCTCCAA CTATGACACT GACAAGAAGT ATTCATTCTG CAATGAGCAG
    CGTGCACTTG TACAGACACG TGGTGGTAAC TCCAATGGAG CTCTTTGCAA CTTTCCATTT
    TTGTACAACA ACCGCAACTA TACAGACTGC ACCTCTGAAG GTCGGAGGGA CAGTATGAAG
    TGGTGCGGGA CTACTGCCAA CTATGATGCC GATCAAAAAT TTGGATTCTG TCCCATGGCA
    GCTCATGAGG AAATATGCAC TACTAATGAG GGAGTCATGT ATAGAGTTGG TGATCAGTGG
    GACAAGCAGC ATGATCAAGG CCACATGATG CGATGTACTT GTGTTGGAAA TGGTCGTGGG
    GAATGGACTT GTGTGGCTTA TTCTCAGCTT AAAGACCAGT GCATTGTGGA TGGGATAACT
    TATGATGTGA ACAGTTCTTT TACGAAACGC CATGAGGAAG GACACATGAT GAACTGTACC
    TGCTATGGGC AGGGTCGTGG CAGATGGAAG TGCGATGCTA TAGACCAATG CCAGGATACT
    GAAACCCGAC AGTTCTATCA GATTGGAGAT TCCTGGGAAA AGCATTTGCA GGGAGTACGG
    TACCAGTGTT ACTGCTATGG CAAAGGAATT GGTGAATGGC ATTGCCAGCC TCTATCAACA
    TCCCCAGCTG GGACTGGACC CGTTCAGGTT ATTATCACAG AGAGTTCCAA TTTCCCTAAT
    TCTCACCCTA TACAGTGGAA TGCCCCACAG CCATCACACA TTAAGAATTA CATCCTGCGC
    TGGAAGCCTA AACTGAAGAC TGGCCCATGG AAACAAGCCA CTATTCCAGG ACACCTGAAC
    TCTTATACCA TCTCTGGCTT GAAACCTGGA ATCCTGTATG AGGGGCAATT GATCAGCATT
    CTGCAGTATG GAAACAGGGA GGTCACAACC TTTGACTTTA CCACCACTTC TACCATTCAC
    CGCTCCAGTC AAACTGAGAC AGGAGAAACT ACCCCACTTC CACCATTGGT GGCCACATCT
    GAGTCTGTAA CAGAAATTAC TGCTAGCAGT TTTCTGGTAT CCTGGGTCTC TGCATCTGAC
    ACAGTGTCTG GCTTCCGTGT TGAATATGAG CTGAGTGAGG ATGGAGATGA GAAACGCTAC
    CTAGAACTTC CAAACACTGC CACTTCAGTC AGTATCCCAG ACCTCCTCCC AGGCCGTAGA
    TACAATGTGA ATGTGTATCA GATAACTGAA GAGGGTGAAA AGAGCCTCAT CCTATCAACT
    ACTCAGACAA CTGCACCTGA TGCACCCCCC GACCATAATG TGGAAAATGT TGATGACACC
    TCCATCCTGA TCAAATGGAC CAAACCTCAA GCTCCCGTCA CAGGATACAG GGTTGTATAC
    AGTCCATCTG TAGAAGGCAG CAGCACAGAG TTAAACCTCC CAAGTACTGC AACTTCAGTG
    AGCCTGACTG AGCTGCTCCC AGGAATTGAA TACAATATTA CAATCTATGC AGTGGAAGAC
    AGTTTAGAAA GTGTTCCCAT CTTTATTCAG CAAGGGACAA CTGGAACTCC ACAGACAGTT
    ATAGTACCAT CTCCCACTGA CTTACAATTA GTGGAAGTTA CCGATATAAA AATTACCATC
    ATGTGGAACC CCCCACAAAG TGAGGTTTCT GGATACCGAG TTGTTGTGAA ACCTGTAAGT
    CCCTCTGACC GTGATGTGCA GAATCTCCCC GTGAGCAGGA ACACTTTTGC CGAGGTGGTT
    AACTTGCACC CTGGCAGGAC CTATACCTTT GAGGTGTATG CAGTTAATCG TGGACAAGAG
    AGTGAACCCC TGGCTGGAGA CTTCACTACC ATGGAACCTG TGGGATCAAT TCCACCTTAC
    AACACTGAAG TCACAGAGAC TACCATAGTG GTAACTTGGA CCCCTGTGCC CAGAATCGGG
    TTTAAGCTGG ATGTTCGACC CAGCCAAGGT GGGGAGGCAC CTCGGGAAGT CATATCCGAG
    TCTGGCAGCA TTGTGATCTC TGGCCTAACC CCTGGAGTCG AATATATGTA CAGCATATCT
    GTTCTTAAGG ATGGCGTTGA GAGGGACATT CCCATCACCA AGACTGTTGT CACACCTGTC
    GCTCCTCCAA CCAACTTGCG CCTCCAACCA AGCAGAGATT CTGCTACACT TACTGTTTAC
    TGGGACAGTA GCATTTCACC AGGAATCACT GGATACAGAA TTACCACTGC ACCCACTCCA
    ATGCAGGTTG GCAATTCCCT GGAAGAGGAG GTTGGTCCAA CTCAGACATA CTGCCTCTTT
    GAGAACCTGA GCCCTGGTGT AGAGTACAAT GTCAGCGTGT ATGCAGTAAA AGGGGAAGAG
    GAGAGTTCAC CTCTCTCCCA GATCTTTCTT CAAGAGATAC CCCAACTGAC TGACCTAAAG
    TATGACGATG TAACTGACAC AAGCATTGAC CTGAGGTGGA CGCCCCTTAA TTCCTCCACC
    ATTATCGGCT ACCGAATCAC GGTGGTTGCG GCGGGAGAAT CCGTCCCTAT CTACGAGGAA
    TTTGTGGGCC CCACAGATGG GTATTACAAA GTTTCAGGAT TGGAACCCGG CATTGACTAT
    GAGATCAGCG TGATAACACT TATTAGCGGC GGAGAGAGCG CCCCGACCAC AATCGTACAG
    CACACGGCTG TTCCACCTCC AACTAATTTG CGCTTCACCA ATGTTGGCCC TGACACCATG
    CGCGTCACCT GGTCACCTCC AACTTCTATC GAACTATCAA GTTTCCTGGT ACGATATTCT
    CCCGTGAAGA AGCCAGATGA TGTGACAGAA CTCTCCCTCT CTCCATCTAC CAACATGGTG
    GTTTTATCAA ACTTGCTTCC CTTCACCGAA TATTTAGTCA GCGTTCATTC TGTTTATGAG
    GAGCGAGAGA GTAGTGCCCT CAATGGTGTA GCTAAGACTT ACTTGGACTC TCCAACTGGC
    ATTGACTTTT CTGAAATTAC TCCCAATTCA TTTACTGTGC ACTGGATTGC TCCACGTGCC
    CCTATCACTG GCTACAGAAT TCGGTACCAG TTAGAAAGTG GAGCTGGGCG ACCCAAGGAG
    GAGCGGGTAC CTCCATCCCG GAACTCTATT ACCCTTACCC ACCTCATTCC AGGTTCAGAA
    TACATTGTCA GTATCATTGC ATTCAATGGT CAACAGGAGA GTTTGCCACT TTCTGGACAG
    CAAGCCACTG TATCTGATGT CCCAACTGAT TTGGAAGTGA CCTCCTCCTC TCCTAACACA
    CTCACCATCT CTTGGGAAGC ACCTTCAGTG AATGTACGTT ACTACAGGAT CACCCATTCC
    CAGACAGGAG GTCATGGTCC AGAGAAGGAG TTTACAGTTC CAGGAACATC CAACACTGCA
    ACCATCAGAG GGTTAAATCC AGGAGTAAGC TACACCATCA CTGTTTATGC AGTGACTGGG
    CGAGGGGATA GTCCTGCCTC TAGCAAACCA CTTACCATTG TTCACATGAC AGATGTTGAC
    CAACCAACTG ACATGGCTGT CACAGATATC CAGGATCACA GCATTCATGT CAAATGGTCT
    CCTCCTCCAG GCCCAGTCAC TGGTTACAGG GTCACTAGTG TCCCAAAAAG TGGCCAAGGA
    GAAACCTTTT CCCAGGTCGT TTCTCCAGAT CAAACTGAAG TGACGATTGT GGGCCTGCAA
    CCAGCTGTGG AGTATGTGGT GAGCATATAC TCACAAGGTG AAAATGGCGA GAGCGAGCCT
    CTGGTTGAAA CTGCAGTTAC AAACATTGAC CATCCCAAAG GACTGACATT CACTGATGTG
    GGTGTTGATT CCATTAGACT GGCTTGGGAG GTCCCAGATG GGCAGGTCAC CCGCTACAGG
    GTTACCTACT CAAGTCCTGA GGAGGGAGTA AAAGAGTTGT TCCCAGCTCC AGAAGGGGAT
    GATGACACAG CAGAATTGCA CGGTCTGAGG CCGGGCACAG AATACACTGT GAGCATCGTG
    GCATTGCACG ATGACATGGA GAGCAAGCCG ATGATTGGAA TCCAGAGCAC AGCAATTCCA
    GCACCAACAA ATCTTCAGTT CTCTCAAGTG ACACCAAGTG GCTTCTCTCT TAGCTGGCAT
    GCACCCACTA TCCATCTCAG TGGGTACCTT GTACGTGTCA ACCCTAAAGA GAAAACTGGG
    CCTATGAAGG AAGTTAGACT TTCACCTGGT GTGACTGCTA CAACTGTAAC TGGTCTGATG
    GTGGCCACAA AATATGAAGT TAATGTGTAT GCTCTTAAGG ATACCCTGAC AAGTCAGCCA
    CTGCAGGGTT TAATCTCTAC ACTTGATAAT GTAAGTCCAC CTCGAAGACC TCGTGTTCAA
    GATGTGACCG AGACCACAGT TACACTGTCC TGGCGCACTA AAACAGAGAC CATCACTGGC
    TTCCAGATTG ATGCTATACC TGCCGGAGGT CAGAATCCCA TCAGGAGAAC AGTTGATGCA
    GATCTCCGTT CTTTTACTAT CACAGGTCTG CAGCCTGGCA CAGACTACAA GATCTATCTG
    TACACCCTAA ATGACAATGC TCGTAGCTCT CCTGTGATTG TTGATGTCAC CACAGCTGTG
    GACTCTCCAT CCAACCTGCG TTTCCTGACC ACAACTAGCA ACTCCCTGCT GTTCACTTGG
    CAGCCACCTC GTGCTCGCAT CACTGGCTAT ATCATTCGTT ATGAGAAAGC TGGAGGGCTA
    CTGAAAGAGC ACCTCCCACG CCTCCAAGCA GGAACCACAG AGTCTACCAT TACCAACCTG
    GAGCCAGGCA CAGAATATAT TGTCTATATT ATTGCTGTGA GAAACAATCA GAAGAGTGAG
    CCTCTTGTGG GACGTAAGAG AACAGACGAA CTGCCTCGCC TGGTTACACT TCCACACCCG
    GGCAAAGGCC CTGAAATCCT TGATGTTCCC ACTGATGAAG AGAACACACC CCACATCACA
    CAAATCAAGT TGGACAATGG TAATGGTATC CAACTGCCAG GCTCTAATGG ACAGCAGCCC
    AGTTCTGGCA ATGAAGGGCA GCTTATAGAA GAACATGGTT TCAGAAGTCC CCTTGCACCC
    ACTACTGCTG TGCCAGTGAG GCCAGGGAAA TTCACCCCTG GGCACTACCC ACAGGAGAGG
    GTTGATATTG AGCTAGACAC AGAGTTCCCT GTGCAGCGTG GAGATCTTGA TGGTCCTTAC
    CCTCATGGCT TAGGACCACA GCTAAATGAT TCTGGCGTCC AAGAAGTGGC ATCCCATACT
    ACCATTTCAT GGAGACCTGA GCTGGAAACC ACTGAATATA TCATCTCTTG CCATCCTATT
    GAACATGATG AGGCCCCATT GCAGTTTAGG GTCCCTGGAA CCTCCTCTAG TGCCACATTA
    AATGGTCTGA CTCAAGGAGC CACTTATAAC ATCATTGTGG AAGCGCAGAA GGGGTCTGAT
    AAACATAAGG TCTTCGAGAA GCGTGTAACT GTTGGCAGTC CTGGTTCCCC TGAGGGGGTT
    TTACAGCCAG CTGAGGACAC CTGTTATGAC ACATTCAATG GAGCACACTA TTCTGTGGGA
    CAGGAATGGG AACGCATGTC AGAATCTGGA TTCAGGCTCT GGTGCAAGTG CCTGGGCTAT
    GGAAGTGGAC ACTTCAGATG TGACTCCTCC AAATGGTGCC ATGATAATGG TGTCAATCAC
    AGGGTCGGGG AGAAATGGGA CCGACGAGGA GAGAATGGTC AGATGATGAG TTGTACTTGT
    CTTGGCAATG GAAAAGGAGA ATTTAAGTGT GAACCTCATG AAGCCACGTG CTATGATGAA
    GGGAAGATGT ACAATGTTGG AGAGCAGTGG CAGAAGGAAT ATTTAGGGGC AATCTGTTCA
    TGCACATGCT ATGGAGGACA GCAAGGGTGG AGATGTGACA ATTGCCGCAG ACCAGGTGCT
    GTTCTTCCCG ATGGCACTGC TGGGCATACA GTCTCTCAGT TTGCCCAGAG ATACCAACAG
    AACTATAATT TGAACTGCCC CATTGAGTGC TACCTTCCCC TCCAAGCAGA TATACAGCAT
    TCGGAACAGA CCCAGAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa25257
    Clone ID Related Accession (Same CDS sequence) NM_001030524.1
    Accession Version NM_001030524.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7428bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product fibronectin precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC095906.1. ##Evidence-Data-START## Transcript exon combination :: BC095906.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCGCTGGG GGGCCCTGAC CGGGCTGCTC CTAGCCCTGT GCCTGTGTGC AGTGGTACGT 
    TCCGCCCCAT CAAGCAAGAA GCGCAGGCAG GCGCAGCAGC AGCAAGTAGT GCAACCCCAT
    GGATCACCGG ACAGCCAGCA GAAAGGATGT TATGACAATG GCAAGTACTA CCAAATCAAC
    CAGCAATGGG AGCGCACCTA CCTAGGGAAC ACACTGGTGT GTACTTGTTA TGGCGGAGGC
    AGAGGTTTCA ACTGCGAAAG CAAACCAGAG TCTGAAGAGA CCTGCTTTGA CAAATACACT
    GGTGTTACTT ACCGGGTCGG TGAAACATAT GAGCGACCAA AGGATAACAT GATCTGGGAC
    TGTACGTGTA TTGGAGCTGG AAGGGGACGC ATTAGCTGTA CAATTGCAAA TCGTTGCCAT
    GAAGGAGGTC AGTCTTATAA GATTGGGGAC ACCTGGCGCA GGCCACATGA AACTGGGGGG
    TATATGCTAG AATGTGTCTG CCTTGGAAAT GGTAAAGGAG AATGGACCTG CAAGCCCGTT
    GCTGAGAGAT GCTATGATAA CACAGCTGGG ATATCCTATG TAGTTGGTCA GACCTGGGAA
    AAGCCTTATC AGGGCTGGAT GATGGTGGAC TGTACATGCT TGGGAGAAGG CAGTGGACGA
    ATCACATGTT CTTCCAAAAA CAGATGCAAT GACCAGGACA CCAGAACTTC TTATAGAATT
    GGAAATTCAT GGAGTAAGAC TGACACCAGA GGAAATCTCT TGCAATGTAT CTGTACTGGC
    AATGGGCGAG GAGAGTGGAA ATGTGATAGA CATTCCTCTG CACAAGCAAC TGGTACTGGC
    TCAAACCCCA TCACAAACAT TCAAACTACC CTCTTCCAGC CTGATTCAGA GCTGGAGCCC
    TATGGTCACT GTGTGACTGA CAATGGGGTC TTGTATTCTT TGGGTATGAG ATGGCTGAAG
    GCACAGGGCA GCAAGCAGAT GCTCTGTACC TGCCTTGGTA ATGGTGTGAG CTGTGAAGAA
    ACTGTTGAAA CCATCACATT TGGTGGTAAT GCCAATGGTG AGCCATGTGC CATTCCTTTC
    ACTCATGATG GCAAGACCTA TTATTCCTGC ACAGGAGAGG GTCGTCAGGA TGGAAAATTG
    TGGTGTGCAA CAACCTCCAA CTATGACACT GACAAGAAGT ATTCATTCTG CAATGAGCAG
    CGTGCACTTG TACAGACACG TGGTGGTAAC TCCAATGGAG CTCTTTGCAA CTTTCCATTT
    TTGTACAACA ACCGCAACTA TACAGACTGC ACCTCTGAAG GTCGGAGGGA CAGTATGAAG
    TGGTGCGGGA CTACTGCCAA CTATGATGCC GATCAAAAAT TTGGATTCTG TCCCATGGCA
    GCTCATGAGG AAATATGCAC TACTAATGAG GGAGTCATGT ATAGAGTTGG TGATCAGTGG
    GACAAGCAGC ATGATCAAGG CCACATGATG CGATGTACTT GTGTTGGAAA TGGTCGTGGG
    GAATGGACTT GTGTGGCTTA TTCTCAGCTT AAAGACCAGT GCATTGTGGA TGGGATAACT
    TATGATGTGA ACAGTTCTTT TACGAAACGC CATGAGGAAG GACACATGAT GAACTGTACC
    TGCTATGGGC AGGGTCGTGG CAGATGGAAG TGCGATGCTA TAGACCAATG CCAGGATACT
    GAAACCCGAC AGTTCTATCA GATTGGAGAT TCCTGGGAAA AGCATTTGCA GGGAGTACGG
    TACCAGTGTT ACTGCTATGG CAAAGGAATT GGTGAATGGC ATTGCCAGCC TCTATCAACA
    TCCCCAGCTG GGACTGGACC CGTTCAGGTT ATTATCACAG AGAGTTCCAA TTTCCCTAAT
    TCTCACCCTA TACAGTGGAA TGCCCCACAG CCATCACACA TTAAGAATTA CATCCTGCGC
    TGGAAGCCTA AACTGAAGAC TGGCCCATGG AAACAAGCCA CTATTCCAGG ACACCTGAAC
    TCTTATACCA TCTCTGGCTT GAAACCTGGA ATCCTGTATG AGGGGCAATT GATCAGCATT
    CTGCAGTATG GAAACAGGGA GGTCACAACC TTTGACTTTA CCACCACTTC TACCATTCAC
    CGCTCCAGTC AAACTGAGAC AGGAGAAACT ACCCCACTTC CACCATTGGT GGCCACATCT
    GAGTCTGTAA CAGAAATTAC TGCTAGCAGT TTTCTGGTAT CCTGGGTCTC TGCATCTGAC
    ACAGTGTCTG GCTTCCGTGT TGAATATGAG CTGAGTGAGG ATGGAGATGA GAAACGCTAC
    CTAGAACTTC CAAACACTGC CACTTCAGTC AGTATCCCAG ACCTCCTCCC AGGCCGTAGA
    TACAATGTGA ATGTGTATCA GATAACTGAA GAGGGTGAAA AGAGCCTCAT CCTATCAACT
    ACTCAGACAA CTGCACCTGA TGCACCCCCC GACCATAATG TGGAAAATGT TGATGACACC
    TCCATCCTGA TCAAATGGAC CAAACCTCAA GCTCCCGTCA CAGGATACAG GGTTGTATAC
    AGTCCATCTG TAGAAGGCAG CAGCACAGAG TTAAACCTCC CAAGTACTGC AACTTCAGTG
    AGCCTGACTG AGCTGCTCCC AGGAATTGAA TACAATATTA CAATCTATGC AGTGGAAGAC
    AGTTTAGAAA GTGTTCCCAT CTTTATTCAG CAAGGGACAA CTGGAACTCC ACAGACAGTT
    ATAGTACCAT CTCCCACTGA CTTACAATTA GTGGAAGTTA CCGATATAAA AATTACCATC
    ATGTGGAACC CCCCACAAAG TGAGGTTTCT GGATACCGAG TTGTTGTGAA ACCTGTAAGT
    CCCTCTGACC GTGATGTGCA GAATCTCCCC GTGAGCAGGA ACACTTTTGC CGAGGTGGTT
    AACTTGCACC CTGGCAGGAC CTATACCTTT GAGGTGTATG CAGTTAATCG TGGACAAGAG
    AGTGAACCCC TGGCTGGAGA CTTCACTACC AAACTCGATG CTCCATCAGA CTTGCAGTTT
    ACAGATGTAA CTGACTCTAC AGTTGTTATT ATCTGGAGAC CACCCCAAGC CAAAATTGGT
    CGTTACCTGC TGTCTGTAGG TCAAACCCGG GGAGGCCAGC CGAGCCAATT TCACATTAAC
    CCCTCTGCCA CAAACCACAA GCTGGAGAGT CTCTCCCCAG GCACAGAGTA CACCATTTCA
    CTTGTTGCCC TCAAAGGCAA TCAGCAAAGT GCTAGCACTA CTGGTGTCTT TTCAACATTG
    GAACCTGTGG GATCAATTCC ACCTTACAAC ACTGAAGTCA CAGAGACTAC CATAGTGGTA
    ACTTGGACCC CTGTGCCCAG AATCGGGTTT AAGCTGGATG TTCGACCCAG CCAAGGTGGG
    GAGGCACCTC GGGAAGTCAT ATCCGAGTCT GGCAGCATTG TGATCTCTGG CCTAACCCCT
    GGAGTCGAAT ATATGTACAG CATATCTGTT CTTAAGGATG GCGTTGAGAG GGACATTCCC
    ATCACCAAGA CTGTTGTCAC ACCTGTCGCT CCTCCAACCA ACTTGCGCCT CCAACCAAGC
    AGAGATTCTG CTACACTTAC TGTTTACTGG GACAGTAGCA TTTCACCAGG AATCACTGGA
    TACAGAATTA CCACTGCACC CACTCCAATG CAGGTTGGCA ATTCCCTGGA AGAGGAGGTT
    GGTCCAACTC AGACATACTG CCTCTTTGAG AACCTGAGCC CTGGTGTAGA GTACAATGTC
    AGCGTGTATG CAGTAAAAGG GGAAGAGGAG AGTTCACCTC TCTCCCAGAT CTTTCTTCAA
    GAGATACCCC AACTGACTGA CCTAAAGTAT GACGATGTAA CTGACACAAG CATTGACCTG
    AGGTGGACGC CCCTTAATTC CTCCACCATT ATCGGCTACC GAATCACGGT GGTTGCGGCG
    GGAGAATCCG TCCCTATCTA CGAGGAATTT GTGGGCCCCA CAGATGGGTA TTACAAAGTT
    TCAGGATTGG AACCCGGCAT TGACTATGAG ATCAGCGTGA TAACACTTAT TAGCGGCGGA
    GAGAGCGCCC CGACCACAAT CGTACAGCAC ACGGCTGTTC CACCTCCAAC TAATTTGCGC
    TTCACCAATG TTGGCCCTGA CACCATGCGC GTCACCTGGT CACCTCCAAC TTCTATCGAA
    CTATCAAGTT TCCTGGTACG ATATTCTCCC GTGAAGAAGC CAGATGATGT GACAGAACTC
    TCCCTCTCTC CATCTACCAA CATGGTGGTT TTATCAAACT TGCTTCCCTT CACCGAATAT
    TTAGTCAGCG TTCATTCTGT TTATGAGGAG CGAGAGAGTA GTGCCCTCAA TGGTGTAGCT
    AAGACTTACT TGGACTCTCC AACTGGCATT GACTTTTCTG AAATTACTCC CAATTCATTT
    ACTGTGCACT GGATTGCTCC ACGTGCCCCT ATCACTGGCT ACAGAATTCG GTACCAGTTA
    GAAAGTGGAG CTGGGCGACC CAAGGAGGAG CGGGTACCTC CATCCCGGAA CTCTATTACC
    CTTACCCACC TCATTCCAGG TTCAGAATAC ATTGTCAGTA TCATTGCATT CAATGGTCAA
    CAGGAGAGTT TGCCACTTTC TGGACAGCAA GCCACTGTAT CTGATGTCCC AACTGATTTG
    GAAGTGACCT CCTCCTCTCC TAACACACTC ACCATCTCTT GGGAAGCACC TTCAGTGAAT
    GTACGTTACT ACAGGATCAC CCATTCCCAG ACAGGAGGTC ATGGTCCAGA GAAGGAGTTT
    ACAGTTCCAG GAACATCCAA CACTGCAACC ATCAGAGGGT TAAATCCAGG AGTAAGCTAC
    ACCATCACTG TTTATGCAGT GACTGGGCGA GGGGATAGTC CTGCCTCTAG CAAACCACTT
    ACCATTGTTC ACATGACAGA TGTTGACCAA CCAACTGACA TGGCTGTCAC AGATATCCAG
    GATCACAGCA TTCATGTCAA ATGGTCTCCT CCTCCAGGCC CAGTCACTGG TTACAGGGTC
    ACTAGTGTCC CAAAAAGTGG CCAAGGAGAA ACCTTTTCCC AGGTCGTTTC TCCAGATCAA
    ACTGAAGTGA CGATTGTGGG CCTGCAACCA GCTGTGGAGT ATGTGGTGAG CATATACTCA
    CAAGGTGAAA ATGGCGAGAG CGAGCCTCTG GTTGAAACTG CAGTTACAAA CATTGACCAT
    CCCAAAGGAC TGACATTCAC TGATGTGGGT GTTGATTCCA TTAGACTGGC TTGGGAGGTC
    CCAGATGGGC AGGTCACCCG CTACAGGGTT ACCTACTCAA GTCCTGAGGA GGGAGTAAAA
    GAGTTGTTCC CAGCTCCAGA AGGGGATGAT GACACAGCAG AATTGCACGG TCTGAGGCCG
    GGCACAGAAT ACACTGTGAG CATCGTGGCA TTGCACGATG ACATGGAGAG CAAGCCGATG
    ATTGGAATCC AGAGCACAGC AATTCCAGCA CCAACAAATC TTCAGTTCTC TCAAGTGACA
    CCAAGTGGCT TCTCTCTTAG CTGGCATGCA CCCACTATCC ATCTCAGTGG GTACCTTGTA
    CGTGTCAACC CTAAAGAGAA AACTGGGCCT ATGAAGGAAG TTAGACTTTC ACCTGGTGTG
    ACTGCTACAA CTGTAACTGG TCTGATGGTG GCCACAAAAT ATGAAGTTAA TGTGTATGCT
    CTTAAGGATA CCCTGACAAG TCAGCCACTG CAGGGTTTAA TCTCTACACT TGATAATGTA
    AGTCCACCTC GAAGACCTCG TGTTCAAGAT GTGACCGAGA CCACAGTTAC ACTGTCCTGG
    CGCACTAAAA CAGAGACCAT CACTGGCTTC CAGATTGATG CTATACCTGC CGGAGGTCAG
    AATCCCATCA GGAGAACAGT TGATGCAGAT CTCCGTTCTT TTACTATCAC AGGTCTGCAG
    CCTGGCACAG ACTACAAGAT CTATCTGTAC ACCCTAAATG ACAATGCTCG TAGCTCTCCT
    GTGATTGTTG ATGTCACCAC AGCTGTGGAC TCTCCATCCA ACCTGCGTTT CCTGACCACA
    ACTAGCAACT CCCTGCTGTT CACTTGGCAG CCACCTCGTG CTCGCATCAC TGGCTATATC
    ATTCGTTATG AGAAAGCTGG AGGGCTACTG AAAGAGCACC TCCCACGCCT CCAAGCAGGA
    ACCACAGAGT CTACCATTAC CAACCTGGAG CCAGGCACAG AATATATTGT CTATATTATT
    GCTGTGAGAA ACAATCAGAA GAGTGAGCCT CTTGTGGGAC GTAAGAGAAC AGACGAACTG
    CCTCGCCTGG TTACACTTCC ACACCCGGGC AAAGGCCCTG AAATCCTTGA TGTTCCCACT
    GATGAAGAGA ACACACCCCA CATCACACAA ATCAAGTTGG ACAATGGTAA TGGTATCCAA
    CTGCCAGGCT CTAATGGACA GCAGCCCAGT TCTGGCAATG AAGGGCAGCT TATAGAAGAA
    CATGGTTTCA GAAGTCCCCT TGCACCCACT ACTGCTGTGC CAGTGAGGCC AGGGAAATTC
    ACCCCTGGGC ACTACCCACA GGAGAGGGTT GATATTGAGC TAGACACAGA GTTCCCTGTG
    CAGCGTGGAG ATCTTGATGG TCCTTACCCT CATGGCTTAG GACCACAGCT AAATGATTCT
    GGCGTCCAAG AAGTGGCATC CCATACTACC ATTTCATGGA GACCTGAGCT GGAAACCACT
    GAATATATCA TCTCTTGCCA TCCTATTGAA CATGATGAGG CCCCATTGCA GTTTAGGGTC
    CCTGGAACCT CCTCTAGTGC CACATTAAAT GGTCTGACTC AAGGAGCCAC TTATAACATC
    ATTGTGGAAG CGCAGAAGGG GTCTGATAAA CATAAGGTCT TCGAGAAGCG TGTAACTGTT
    GGCAGTCCTG GTTCCCCTGA GGGGGTTTTA CAGCCAGCTG AGGACACCTG TTATGACACA
    TTCAATGGAG CACACTATTC TGTGGGACAG GAATGGGAAC GCATGTCAGA ATCTGGATTC
    AGGCTCTGGT GCAAGTGCCT GGGCTATGGA AGTGGACACT TCAGATGTGA CTCCTCCAAA
    TGGTGCCATG ATAATGGTGT CAATCACAGG GTCGGGGAGA AATGGGACCG ACGAGGAGAG
    AATGGTCAGA TGATGAGTTG TACTTGTCTT GGCAATGGAA AAGGAGAATT TAAGTGTGAA
    CCTCATGAAG CCACGTGCTA TGATGAAGGG AAGATGTACA ATGTTGGAGA GCAGTGGCAG
    AAGGAATATT TAGGGGCAAT CTGTTCATGC ACATGCTATG GAGGACAGCA AGGGTGGAGA
    TGTGACAATT GCCGCAGACC AGGTGCTGTT CTTCCCGATG GCACTGCTGG GCATACAGTC
    TCTCAGTTTG CCCAGAGATA CCAACAGAAC TATAATTTGA ACTGCCCCAT TGAGTGCTAC
    CTTCCCCTCC AAGCAGATAT ACAGCATTCG GAACAGACCC AGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001025695.1
    CDS194..7621
    Translation

    Target ORF information:

    RefSeq Version NM_001030524.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis fibronectin 1 (fn1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001030524.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCGCTGGG GGGCCCTGAC CGGGCTGCTC CTAGCCCTGT GCCTGTGTGC AGTGGTACGT 
    TCCGCCCCAT CAAGCAAGAA GCGCAGGCAG GCGCAGCAGC AGCAAGTAGT GCAACCCCAT
    GGATCACCGG ACAGCCAGCA GAAAGGATGT TATGACAATG GCAAGTACTA CCAAATCAAC
    CAGCAATGGG AGCGCACCTA CCTAGGGAAC ACACTGGTGT GTACTTGTTA TGGCGGAGGC
    AGAGGTTTCA ACTGCGAAAG CAAACCAGAG TCTGAAGAGA CCTGCTTTGA CAAATACACT
    GGTGTTACTT ACCGGGTCGG TGAAACATAT GAGCGACCAA AGGATAACAT GATCTGGGAC
    TGTACGTGTA TTGGAGCTGG AAGGGGACGC ATTAGCTGTA CAATTGCAAA TCGTTGCCAT
    GAAGGAGGTC AGTCTTATAA GATTGGGGAC ACCTGGCGCA GGCCACATGA AACTGGGGGG
    TATATGCTAG AATGTGTCTG CCTTGGAAAT GGTAAAGGAG AATGGACCTG CAAGCCCGTT
    GCTGAGAGAT GCTATGATAA CACAGCTGGG ATATCCTATG TAGTTGGTCA GACCTGGGAA
    AAGCCTTATC AGGGCTGGAT GATGGTGGAC TGTACATGCT TGGGAGAAGG CAGTGGACGA
    ATCACATGTT CTTCCAAAAA CAGATGCAAT GACCAGGACA CCAGAACTTC TTATAGAATT
    GGAAATTCAT GGAGTAAGAC TGACACCAGA GGAAATCTCT TGCAATGTAT CTGTACTGGC
    AATGGGCGAG GAGAGTGGAA ATGTGATAGA CATTCCTCTG CACAAGCAAC TGGTACTGGC
    TCAAACCCCA TCACAAACAT TCAAACTACC CTCTTCCAGC CTGATTCAGA GCTGGAGCCC
    TATGGTCACT GTGTGACTGA CAATGGGGTC TTGTATTCTT TGGGTATGAG ATGGCTGAAG
    GCACAGGGCA GCAAGCAGAT GCTCTGTACC TGCCTTGGTA ATGGTGTGAG CTGTGAAGAA
    ACTGTTGAAA CCATCACATT TGGTGGTAAT GCCAATGGTG AGCCATGTGC CATTCCTTTC
    ACTCATGATG GCAAGACCTA TTATTCCTGC ACAGGAGAGG GTCGTCAGGA TGGAAAATTG
    TGGTGTGCAA CAACCTCCAA CTATGACACT GACAAGAAGT ATTCATTCTG CAATGAGCAG
    CGTGCACTTG TACAGACACG TGGTGGTAAC TCCAATGGAG CTCTTTGCAA CTTTCCATTT
    TTGTACAACA ACCGCAACTA TACAGACTGC ACCTCTGAAG GTCGGAGGGA CAGTATGAAG
    TGGTGCGGGA CTACTGCCAA CTATGATGCC GATCAAAAAT TTGGATTCTG TCCCATGGCA
    GCTCATGAGG AAATATGCAC TACTAATGAG GGAGTCATGT ATAGAGTTGG TGATCAGTGG
    GACAAGCAGC ATGATCAAGG CCACATGATG CGATGTACTT GTGTTGGAAA TGGTCGTGGG
    GAATGGACTT GTGTGGCTTA TTCTCAGCTT AAAGACCAGT GCATTGTGGA TGGGATAACT
    TATGATGTGA ACAGTTCTTT TACGAAACGC CATGAGGAAG GACACATGAT GAACTGTACC
    TGCTATGGGC AGGGTCGTGG CAGATGGAAG TGCGATGCTA TAGACCAATG CCAGGATACT
    GAAACCCGAC AGTTCTATCA GATTGGAGAT TCCTGGGAAA AGCATTTGCA GGGAGTACGG
    TACCAGTGTT ACTGCTATGG CAAAGGAATT GGTGAATGGC ATTGCCAGCC TCTATCAACA
    TCCCCAGCTG GGACTGGACC CGTTCAGGTT ATTATCACAG AGAGTTCCAA TTTCCCTAAT
    TCTCACCCTA TACAGTGGAA TGCCCCACAG CCATCACACA TTAAGAATTA CATCCTGCGC
    TGGAAGCCTA AACTGAAGAC TGGCCCATGG AAACAAGCCA CTATTCCAGG ACACCTGAAC
    TCTTATACCA TCTCTGGCTT GAAACCTGGA ATCCTGTATG AGGGGCAATT GATCAGCATT
    CTGCAGTATG GAAACAGGGA GGTCACAACC TTTGACTTTA CCACCACTTC TACCATTCAC
    CGCTCCAGTC AAACTGAGAC AGGAGAAACT ACCCCACTTC CACCATTGGT GGCCACATCT
    GAGTCTGTAA CAGAAATTAC TGCTAGCAGT TTTCTGGTAT CCTGGGTCTC TGCATCTGAC
    ACAGTGTCTG GCTTCCGTGT TGAATATGAG CTGAGTGAGG ATGGAGATGA GAAACGCTAC
    CTAGAACTTC CAAACACTGC CACTTCAGTC AGTATCCCAG ACCTCCTCCC AGGCCGTAGA
    TACAATGTGA ATGTGTATCA GATAACTGAA GAGGGTGAAA AGAGCCTCAT CCTATCAACT
    ACTCAGACAA CTGCACCTGA TGCACCCCCC GACCATAATG TGGAAAATGT TGATGACACC
    TCCATCCTGA TCAAATGGAC CAAACCTCAA GCTCCCGTCA CAGGATACAG GGTTGTATAC
    AGTCCATCTG TAGAAGGCAG CAGCACAGAG TTAAACCTCC CAAGTACTGC AACTTCAGTG
    AGCCTGACTG AGCTGCTCCC AGGAATTGAA TACAATATTA CAATCTATGC AGTGGAAGAC
    AGTTTAGAAA GTGTTCCCAT CTTTATTCAG CAAGGGACAA CTGGAACTCC ACAGACAGTT
    ATAGTACCAT CTCCCACTGA CTTACAATTA GTGGAAGTTA CCGATATAAA AATTACCATC
    ATGTGGAACC CCCCACAAAG TGAGGTTTCT GGATACCGAG TTGTTGTGAA ACCTGTAAGT
    CCCTCTGACC GTGATGTGCA GAATCTCCCC GTGAGCAGGA ACACTTTTGC CGAGGTGGTT
    AACTTGCACC CTGGCAGGAC CTATACCTTT GAGGTGTATG CAGTTAATCG TGGACAAGAG
    AGTGAACCCC TGGCTGGAGA CTTCACTACC AAACTCGATG CTCCATCAGA CTTGCAGTTT
    ACAGATGTAA CTGACTCTAC AGTTGTTATT ATCTGGAGAC CACCCCAAGC CAAAATTGGT
    CGTTACCTGC TGTCTGTAGG TCAAACCCGG GGAGGCCAGC CGAGCCAATT TCACATTAAC
    CCCTCTGCCA CAAACCACAA GCTGGAGAGT CTCTCCCCAG GCACAGAGTA CACCATTTCA
    CTTGTTGCCC TCAAAGGCAA TCAGCAAAGT GCTAGCACTA CTGGTGTCTT TTCAACATTG
    GAACCTGTGG GATCAATTCC ACCTTACAAC ACTGAAGTCA CAGAGACTAC CATAGTGGTA
    ACTTGGACCC CTGTGCCCAG AATCGGGTTT AAGCTGGATG TTCGACCCAG CCAAGGTGGG
    GAGGCACCTC GGGAAGTCAT ATCCGAGTCT GGCAGCATTG TGATCTCTGG CCTAACCCCT
    GGAGTCGAAT ATATGTACAG CATATCTGTT CTTAAGGATG GCGTTGAGAG GGACATTCCC
    ATCACCAAGA CTGTTGTCAC ACCTGTCGCT CCTCCAACCA ACTTGCGCCT CCAACCAAGC
    AGAGATTCTG CTACACTTAC TGTTTACTGG GACAGTAGCA TTTCACCAGG AATCACTGGA
    TACAGAATTA CCACTGCACC CACTCCAATG CAGGTTGGCA ATTCCCTGGA AGAGGAGGTT
    GGTCCAACTC AGACATACTG CCTCTTTGAG AACCTGAGCC CTGGTGTAGA GTACAATGTC
    AGCGTGTATG CAGTAAAAGG GGAAGAGGAG AGTTCACCTC TCTCCCAGAT CTTTCTTCAA
    GAGATACCCC AACTGACTGA CCTAAAGTAT GACGATGTAA CTGACACAAG CATTGACCTG
    AGGTGGACGC CCCTTAATTC CTCCACCATT ATCGGCTACC GAATCACGGT GGTTGCGGCG
    GGAGAATCCG TCCCTATCTA CGAGGAATTT GTGGGCCCCA CAGATGGGTA TTACAAAGTT
    TCAGGATTGG AACCCGGCAT TGACTATGAG ATCAGCGTGA TAACACTTAT TAGCGGCGGA
    GAGAGCGCCC CGACCACAAT CGTACAGCAC ACGGCTGTTC CACCTCCAAC TAATTTGCGC
    TTCACCAATG TTGGCCCTGA CACCATGCGC GTCACCTGGT CACCTCCAAC TTCTATCGAA
    CTATCAAGTT TCCTGGTACG ATATTCTCCC GTGAAGAAGC CAGATGATGT GACAGAACTC
    TCCCTCTCTC CATCTACCAA CATGGTGGTT TTATCAAACT TGCTTCCCTT CACCGAATAT
    TTAGTCAGCG TTCATTCTGT TTATGAGGAG CGAGAGAGTA GTGCCCTCAA TGGTGTAGCT
    AAGACTTACT TGGACTCTCC AACTGGCATT GACTTTTCTG AAATTACTCC CAATTCATTT
    ACTGTGCACT GGATTGCTCC ACGTGCCCCT ATCACTGGCT ACAGAATTCG GTACCAGTTA
    GAAAGTGGAG CTGGGCGACC CAAGGAGGAG CGGGTACCTC CATCCCGGAA CTCTATTACC
    CTTACCCACC TCATTCCAGG TTCAGAATAC ATTGTCAGTA TCATTGCATT CAATGGTCAA
    CAGGAGAGTT TGCCACTTTC TGGACAGCAA GCCACTGTAT CTGATGTCCC AACTGATTTG
    GAAGTGACCT CCTCCTCTCC TAACACACTC ACCATCTCTT GGGAAGCACC TTCAGTGAAT
    GTACGTTACT ACAGGATCAC CCATTCCCAG ACAGGAGGTC ATGGTCCAGA GAAGGAGTTT
    ACAGTTCCAG GAACATCCAA CACTGCAACC ATCAGAGGGT TAAATCCAGG AGTAAGCTAC
    ACCATCACTG TTTATGCAGT GACTGGGCGA GGGGATAGTC CTGCCTCTAG CAAACCACTT
    ACCATTGTTC ACATGACAGA TGTTGACCAA CCAACTGACA TGGCTGTCAC AGATATCCAG
    GATCACAGCA TTCATGTCAA ATGGTCTCCT CCTCCAGGCC CAGTCACTGG TTACAGGGTC
    ACTAGTGTCC CAAAAAGTGG CCAAGGAGAA ACCTTTTCCC AGGTCGTTTC TCCAGATCAA
    ACTGAAGTGA CGATTGTGGG CCTGCAACCA GCTGTGGAGT ATGTGGTGAG CATATACTCA
    CAAGGTGAAA ATGGCGAGAG CGAGCCTCTG GTTGAAACTG CAGTTACAAA CATTGACCAT
    CCCAAAGGAC TGACATTCAC TGATGTGGGT GTTGATTCCA TTAGACTGGC TTGGGAGGTC
    CCAGATGGGC AGGTCACCCG CTACAGGGTT ACCTACTCAA GTCCTGAGGA GGGAGTAAAA
    GAGTTGTTCC CAGCTCCAGA AGGGGATGAT GACACAGCAG AATTGCACGG TCTGAGGCCG
    GGCACAGAAT ACACTGTGAG CATCGTGGCA TTGCACGATG ACATGGAGAG CAAGCCGATG
    ATTGGAATCC AGAGCACAGC AATTCCAGCA CCAACAAATC TTCAGTTCTC TCAAGTGACA
    CCAAGTGGCT TCTCTCTTAG CTGGCATGCA CCCACTATCC ATCTCAGTGG GTACCTTGTA
    CGTGTCAACC CTAAAGAGAA AACTGGGCCT ATGAAGGAAG TTAGACTTTC ACCTGGTGTG
    ACTGCTACAA CTGTAACTGG TCTGATGGTG GCCACAAAAT ATGAAGTTAA TGTGTATGCT
    CTTAAGGATA CCCTGACAAG TCAGCCACTG CAGGGTTTAA TCTCTACACT TGATAATGTA
    AGTCCACCTC GAAGACCTCG TGTTCAAGAT GTGACCGAGA CCACAGTTAC ACTGTCCTGG
    CGCACTAAAA CAGAGACCAT CACTGGCTTC CAGATTGATG CTATACCTGC CGGAGGTCAG
    AATCCCATCA GGAGAACAGT TGATGCAGAT CTCCGTTCTT TTACTATCAC AGGTCTGCAG
    CCTGGCACAG ACTACAAGAT CTATCTGTAC ACCCTAAATG ACAATGCTCG TAGCTCTCCT
    GTGATTGTTG ATGTCACCAC AGCTGTGGAC TCTCCATCCA ACCTGCGTTT CCTGACCACA
    ACTAGCAACT CCCTGCTGTT CACTTGGCAG CCACCTCGTG CTCGCATCAC TGGCTATATC
    ATTCGTTATG AGAAAGCTGG AGGGCTACTG AAAGAGCACC TCCCACGCCT CCAAGCAGGA
    ACCACAGAGT CTACCATTAC CAACCTGGAG CCAGGCACAG AATATATTGT CTATATTATT
    GCTGTGAGAA ACAATCAGAA GAGTGAGCCT CTTGTGGGAC GTAAGAGAAC AGACGAACTG
    CCTCGCCTGG TTACACTTCC ACACCCGGGC AAAGGCCCTG AAATCCTTGA TGTTCCCACT
    GATGAAGAGA ACACACCCCA CATCACACAA ATCAAGTTGG ACAATGGTAA TGGTATCCAA
    CTGCCAGGCT CTAATGGACA GCAGCCCAGT TCTGGCAATG AAGGGCAGCT TATAGAAGAA
    CATGGTTTCA GAAGTCCCCT TGCACCCACT ACTGCTGTGC CAGTGAGGCC AGGGAAATTC
    ACCCCTGGGC ACTACCCACA GGAGAGGGTT GATATTGAGC TAGACACAGA GTTCCCTGTG
    CAGCGTGGAG ATCTTGATGG TCCTTACCCT CATGGCTTAG GACCACAGCT AAATGATTCT
    GGCGTCCAAG AAGTGGCATC CCATACTACC ATTTCATGGA GACCTGAGCT GGAAACCACT
    GAATATATCA TCTCTTGCCA TCCTATTGAA CATGATGAGG CCCCATTGCA GTTTAGGGTC
    CCTGGAACCT CCTCTAGTGC CACATTAAAT GGTCTGACTC AAGGAGCCAC TTATAACATC
    ATTGTGGAAG CGCAGAAGGG GTCTGATAAA CATAAGGTCT TCGAGAAGCG TGTAACTGTT
    GGCAGTCCTG GTTCCCCTGA GGGGGTTTTA CAGCCAGCTG AGGACACCTG TTATGACACA
    TTCAATGGAG CACACTATTC TGTGGGACAG GAATGGGAAC GCATGTCAGA ATCTGGATTC
    AGGCTCTGGT GCAAGTGCCT GGGCTATGGA AGTGGACACT TCAGATGTGA CTCCTCCAAA
    TGGTGCCATG ATAATGGTGT CAATCACAGG GTCGGGGAGA AATGGGACCG ACGAGGAGAG
    AATGGTCAGA TGATGAGTTG TACTTGTCTT GGCAATGGAA AAGGAGAATT TAAGTGTGAA
    CCTCATGAAG CCACGTGCTA TGATGAAGGG AAGATGTACA ATGTTGGAGA GCAGTGGCAG
    AAGGAATATT TAGGGGCAAT CTGTTCATGC ACATGCTATG GAGGACAGCA AGGGTGGAGA
    TGTGACAATT GCCGCAGACC AGGTGCTGTT CTTCCCGATG GCACTGCTGG GCATACAGTC
    TCTCAGTTTG CCCAGAGATA CCAACAGAAC TATAATTTGA ACTGCCCCAT TGAGTGCTAC
    CTTCCCCTCC AAGCAGATAT ACAGCATTCG GAACAGACCC AGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa28344
    Clone ID Related Accession (Same CDS sequence) XM_012970493.3 , XM_012970493.2
    Accession Version XM_012970493.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product fibronectin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970493.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCGCTGGG GGGCCCTGAC CGGGCTGCTC CTAGCCCTGT GCCTGTGTGC AGTGGTACGT 
    TCCGCCCCAT CAAGCAAGAA GCGCAGGCAG GCGCAGCAGC AGCAAGTAGT GCAACCCCAT
    GGATCACCGG ACAGCCAGCA GAAAGGATGT TATGACAATG GCAAGTACTA CCAAATCAAC
    CAGCAATGGG AGCGCACCTA CCTAGGGAAC ACACTGGTGT GTACTTGTTA TGGCGGAGGC
    AGAGGTTTCA ACTGCGAAAG CAAACCAGAG TCTGAAGAGA CCTGCTTTGA CAAATACACT
    GGTGTTACTT ACCGGGTCGG TGAAACATAT GAGCGACCAA AGGATAACAT GATCTGGGAC
    TGTACGTGTA TTGGAGCTGG AAGGGGACGC ATTAGCTGTA CAATTGCAAA TCGTTGCCAT
    GAAGGAGGTC AGTCTTATAA GATTGGGGAC ACCTGGCGCA GGCCACATGA AACTGGGGGG
    TATATGCTAG AATGTGTCTG CCTTGGAAAT GGTAAAGGAG AATGGACCTG CAAGCCCGTT
    GCTGAGAGAT GCTATGATAA CACAGCTGGG ATATCCTATG TAGTTGGTCA GACCTGGGAA
    AAGCCTTATC AGGGCTGGAT GATGGTGGAC TGTACATGCT TGGGAGAAGG CAGTGGACGA
    ATCACATGTT CTTCCAAAAA CAGATGCAAT GACCAGGACA CCAGAACTTC TTATAGAATT
    GGAAATTCAT GGAGTAAGAC TGACACCAGA GGAAATCTCT TGCAATGTAT CTGTACTGGC
    AATGGGCGAG GAGAGTGGAA ATGTGATAGA CATTCCTCTG CACAAGCAAC TGGTACTGGC
    TCAAACCCCA TCACAAACAT TCAAACTACC CTCTTCCAGC CTGATTCAGA GCTGGAGCCC
    TATGGTCACT GTGTGACTGA CAATGGGGTC TTGTATTCTT TGGGTATGAG ATGGCTGAAG
    GCACAGGGCA GCAAGCAGAT GCTCTGTACC TGCCTTGGTA ATGGTGTGAG CTGTGAAGAA
    ACTGTTGAAA CCATCACATT TGGTGGTAAT GCCAATGGTG AGCCATGTGC CATTCCTTTC
    ACTCATGATG GCAAGACCTA TTATTCCTGC ACAGGAGAGG GTCGTCAGGA TGGAAAATTG
    TGGTGTGCAA CAACCTCCAA CTATGACACT GACAAGAAGT ATTCATTCTG CAATGAGCAG
    CGTGCACTTG TACAGACACG TGGTGGTAAC TCCAATGGAG CTCTTTGCAA CTTTCCATTT
    TTGTACAACA ACCGCAACTA TACAGACTGC ACCTCTGAAG GTCGGAGGGA CAGTATGAAG
    TGGTGCGGGA CTACTGCCAA CTATGATGCC GATCAAAAAT TTGGATTCTG TCCCATGGCA
    GCTCATGAGG AAATATGCAC TACTAATGAG GGAGTCATGT ATAGAGTTGG TGATCAGTGG
    GACAAGCAGC ATGATCAAGG CCACATGATG CGATGTACTT GTGTTGGAAA TGGTCGTGGG
    GAATGGACTT GTGTGGCTTA TTCTCAGCTT AAAGACCAGT GCATTGTGGA TGGGATAACT
    TATGATGTGA ACAGTTCTTT TACGAAACGC CATGAGGAAG GACACATGAT GAACTGTACC
    TGCTATGGGC AGGGTCGTGG CAGATGGAAG TGCGATGCTA TAGACCAATG CCAGGATACT
    GAAACCCGAC AGTTCTATCA GATTGGAGAT TCCTGGGAAA AGCATTTGCA GGGAGTACGG
    TACCAGTGTT ACTGCTATGG CAAAGGAATT GGTGAATGGC ATTGCCAGCC TCTATCAACA
    TCCCCAGCTG GGACTGGACC CGTTCAGGTT ATTATCACAG AGAGTTCCAA TTTCCCTAAT
    TCTCACCCTA TACAGTGGAA TGCCCCACAG CCATCACACA TTAAGAATTA CATCCTGCGC
    TGGAAGCCTA AACTGAAGAC TGGCCCATGG AAACAAGCCA CTATTCCAGG ACACCTGAAC
    TCTTATACCA TCTCTGGCTT GAAACCTGGA ATCCTGTATG AGGGGCAATT GATCAGCATT
    CTGCAGTATG GAAACAGGGA GGTCACAACC TTTGACTTTA CCACCACTTC TACCATTCAC
    CGCTCCAGTC AAACTGAGAC AGGAGAAACT ACCCCACTTC CACCATTGGT GGCCACATCT
    GAGTCTGTAA CAGAAATTAC TGCTAGCAGT TTTCTGGTAT CCTGGGTCTC TGCATCTGAC
    ACAGTGTCTG GCTTCCGTGT TGAATATGAG CTGAGTGAGG ATGGAGATGA GAAACGCTAC
    CTAGAACTTC CAAACACTGC CACTTCAGTC AGTATCCCAG ACCTCCTCCC AGGCCGTAGA
    TACAATGTGA ATGTGTATCA GATAACTGAA GAGGGTGAAA AGAGCCTCAT CCTATCAACT
    ACTCAGACAA CTGCACCTGA TGCACCCCCC GACCATAATG TGGAAAATGT TGATGACACC
    TCCATCCTGA TCAAATGGAC CAAACCTCAA GCTCCCGTCA CAGGATACAG GGTTGTATAC
    AGTCCATCTG TAGAAGGCAG CAGCACAGAG TTAAACCTCC CAAGTACTGC AACTTCAGTG
    AGCCTGACTG AGCTGCTCCC AGGAATTGAA TACAATATTA CAATCTATGC AGTGGAAGAC
    AGTTTAGAAA GTGTTCCCAT CTTTATTCAG CAAGGGACAA CTGGAACTCC ACAGACAGTT
    ATAGTACCAT CTCCCACTGA CTTACAATTA GTGGAAGTTA CCGATATAAA AATTACCATC
    ATGTGGAACC CCCCACAAAG TGAGGTTTCT GGATACCGAG TTGTTGTGAA ACCTGTAAGT
    CCCTCTGACC GTGATGTGCA GAATCTCCCC GTGAGCAGGA ACACTTTTGC CGAGGTGGTT
    AACTTGCACC CTGGCAGGAC CTATACCTTT GAGGTGTATG CAGTTAATCG TGGACAAGAG
    AGTGAACCCC TGGCTGGAGA CTTCACTACC AAACTCGATG CTCCATCAGA CTTGCAGTTT
    ACAGATGTAA CTGACTCTAC AGTTGTTATT ATCTGGAGAC CACCCCAAGC CAAAATTGGT
    CGTTACCTGC TGTCTGTAGG TCAAACCCGG GGAGGCCAGC CGAGCCAATT TCACATTAAC
    CCCTCTGCCA CAAACCACAA GCTGGAGAGT CTCTCCCCAG GCACAGAGTA CACCATTTCA
    CTTGTTGCCC TCAAAGGCAA TCAGCAAAGT GCTAGCACTA CTGGTGTCTT TTCAACATTG
    GAACCTGTGG GATCAATTCC ACCTTACAAC ACTGAAGTCA CAGAGACTAC CATAGTGGTA
    ACTTGGACCC CTGTGCCCAG AATCGGGTTT AAGCTGGATG TTCGACCCAG CCAAGGTGGG
    GAGGCACCTC GGGAAGTCAT ATCCGAGTCT GGCAGCATTG TGATCTCTGG CCTAACCCCT
    GGAGTCGAAT ATATGTACAG CATATCTGTT CTTAAGGATG GCGTTGAGAG GGACATTCCC
    ATCACCAAGA CTGTTGTCAC ACCTGTCGCT CCTCCAACCA ACTTGCGCCT CCAACCAAGC
    AGAGATTCTG CTACACTTAC TGTTTACTGG GACAGTAGCA TTTCACCAGG AATCACTGGA
    TACAGAATTA CCACTGCACC CACTCCAATG CAGGTTGGCA ATTCCCTGGA AGAGGAGGTT
    GGTCCAACTC AGACATACTG CCTCTTTGAG AACCTGAGCC CTGGTGTAGA GTACAATGTC
    AGCGTGTATG CAGTAAAAGG GGAAGAGGAG AGTTCACCTC TCTCCCAGAT CTTTCTTCAA
    GAGATACCCC AACTGACTGA CCTAAAGTAT GACGATGTAA CTGACACAAG CATTGACCTG
    AGGTGGACGC CCCTTAATTC CTCCACCATT ATCGGCTACC GAATCACGGT GGTTGCGGCG
    GGAGAATCCG TCCCTATCTA CGAGGAATTT GTGGGCCCCA CAGATGGGTA TTACAAAGTT
    TCAGGATTGG AACCCGGCAT TGACTATGAG ATCAGCGTGA TAACACTTAT TAGCGGCGGA
    GAGAGCGCCC CGACCACAAT CGTACAGCAC ACGGCTGTTC CACCTCCAAC TAATTTGCGC
    TTCACCAATG TTGGCCCTGA CACCATGCGC GTCACCTGGT CACCTCCAAC TTCTATCGAA
    CTATCAAGTT TCCTGGTACG ATATTCTCCC GTGAAGAAGC CAGATGATGT GACAGAACTC
    TCCCTCTCTC CATCTACCAA CATGGTGGTT TTATCAAACT TGCTTCCCTT CACCGAATAT
    TTAGTCAGCG TTCATTCTGT TTATGAGGAG CGAGAGAGTA GTGCCCTCAA TGGTGTAGCT
    AAGACTTACT TGGACTCTCC AACTGGCATT GACTTTTCTG AAATTACTCC CAATTCATTT
    ACTGTGCACT GGATTGCTCC ACGTGCCCCT ATCACTGGCT ACAGAATTCG GTACCAGTTA
    GAAAGTGGAG CTGGGCGACC CAAGGAGGAG CGGGTACCTC CATCCCGGAA CTCTATTACC
    CTTACCCACC TCATTCCAGG TTCAGAATAC ATTGTCAGTA TCATTGCATT CAATGGTCAA
    CAGGAGAGTT TGCCACTTTC TGGACAGCAA GCCACTGTAT CTGATGTCCC AACTGATTTG
    GAAGTGACCT CCTCCTCTCC TAACACACTC ACCATCTCTT GGGAAGCACC TTCAGTGAAT
    GTACGTTACT ACAGGATCAC CCATTCCCAG ACAGGAGGTC ATGGTCCAGA GAAGGAGTTT
    ACAGTTCCAG GAACATCCAA CACTGCAACC ATCAGAGGGT TAAATCCAGG AGTAAGCTAC
    ACCATCACTG TTTATGCAGT GACTGGGCGA GGGGATAGTC CTGCCTCTAG CAAACCACTT
    ACCATTGTTC ACATGACAGA TGTTGACCAA CCAACTGACA TGGCTGTCAC AGATATCCAG
    GATCACAGCA TTCATGTCAA ATGGTCTCCT CCTCCAGGCC CAGTCACTGG TTACAGGGTC
    ACTAGTGTCC CAAAAAGTGG CCAAGGAGAA ACCTTTTCCC AGGTCGTTTC TCCAGATCAA
    ACTGAAGTGA CGATTGTGGG CCTGCAACCA GCTGTGGAGT ATGTGGTGAG CATATACTCA
    CAAGGTGAAA ATGGCGAGAG CGAGCCTCTG GTTGAAACTG CAGTTACAAC AATTCCAGCA
    CCAACAAATC TTCAGTTCTC TCAAGTGACA CCAAGTGGCT TCTCTCTTAG CTGGCATGCA
    CCCACTATCC ATCTCAGTGG GTACCTTGTA CGTGTCAACC CTAAAGAGAA AACTGGGCCT
    ATGAAGGAAG TTAGACTTTC ACCTGGTGTG ACTGCTACAA CTGTAACTGG TCTGATGGTG
    GCCACAAAAT ATGAAGTTAA TGTGTATGCT CTTAAGGATA CCCTGACAAG TCAGCCACTG
    CAGGGTTTAA TCTCTACACT TGATAATGTA AGTCCACCTC GAAGACCTCG TGTTCAAGAT
    GTGACCGAGA CCACAGTTAC ACTGTCCTGG CGCACTAAAA CAGAGACCAT CACTGGCTTC
    CAGATTGATG CTATACCTGC CGGAGGTCAG AATCCCATCA GGAGAACAGT TGATGCAGAT
    CTCCGTTCTT TTACTATCAC AGGTCTGCAG CCTGGCACAG ACTACAAGAT CTATCTGTAC
    ACCCTAAATG ACAATGCTCG TAGCTCTCCT GTGATTGTTG ATGTCACCAC AGCTGTGGAC
    TCTCCATCCA ACCTGCGTTT CCTGACCACA ACTAGCAACT CCCTGCTGTT CACTTGGCAG
    CCACCTCGTG CTCGCATCAC TGGCTATATC ATTCGTTATG AGAAAGCTGG AGGGCTACTG
    AAAGAGCACC TCCCACGCCT CCAAGCAGGA ACCACAGAGT CTACCATTAC CAACCTGGAG
    CCAGGCACAG AATATATTGT CTATATTATT GCTGTGAGAA ACAATCAGAA GAGTGAGCCT
    CTTGTGGGAC GTAAGAGAAC AGACGAACTG CCTCGCCTGG TTACACTTCC ACACCCGGGC
    AAAGGCCCTG AAATCCTTGA TGTTCCCACT GATGAAGAGA ACACACCCCA CATCACACAA
    ATCAAGTTGG ACAATGGTAA TGGTATCCAA CTGCCAGGCT CTAATGGACA GCAGCCCAGT
    TCTGGCAATG AAGGGCAGCT TATAGAAGAA CATGGTTTCA GAAGTCCCCT TGCACCCACT
    ACTGCTGTGC CAGTGAGGCC AGGGAAATTC ACCCCTGGGC ACTACCCACA GGAGAGGGTT
    GATATTGAGC TAGACACAGA GTTCCCTGTG CAGCGTGGAG ATCTTGATGG TCCTTACCCT
    CATGGCTTAG GACCACAGCT AAATGATTCT GGCGTCCAAG AAGTGGCATC CCATACTACC
    ATTTCATGGA GACCTGAGCT GGAAACCACT GAATATATCA TCTCTTGCCA TCCTATTGAA
    CATGATGAGG CCCCATTGCA GTTTAGGGTC CCTGGAACCT CCTCTAGTGC CACATTAAAT
    GGTCTGACTC AAGGAGCCAC TTATAACATC ATTGTGGAAG CGCAGAAGGG GTCTGATAAA
    CATAAGGTCT TCGAGAAGCG TGTAACTGTT GGCAGTCCTG GTTCCCCTGA GGGGGTTTTA
    CAGCCAGCTG AGGACACCTG TTATGACACA TTCAATGGAG CACACTATTC TGTGGGACAG
    GAATGGGAAC GCATGTCAGA ATCTGGATTC AGGCTCTGGT GCAAGTGCCT GGGCTATGGA
    AGTGGACACT TCAGATGTGA CTCCTCCAAA TGGTGCCATG ATAATGGTGT CAATCACAGG
    GTCGGGGAGA AATGGGACCG ACGAGGAGAG AATGGTCAGA TGATGAGTTG TACTTGTCTT
    GGCAATGGAA AAGGAGAATT TAAGTGTGAA CCTCATGAAG CCACGTGCTA TGATGAAGGG
    AAGATGTACA ATGTTGGAGA GCAGTGGCAG AAGGAATATT TAGGGGCAAT CTGTTCATGC
    ACATGCTATG GAGGACAGCA AGGGTGGAGA TGTGACAATT GCCGCAGACC AGGTGCTGTT
    CTTCCCGATG GCACTGCTGG GCATACAGTC TCTCAGTTTG CCCAGAGATA CCAACAGAAC
    TATAATTTGA ACTGCCCCAT TGAGTGCTAC CTTCCCCTCC AAGCAGATAT ACAGCATTCG
    GAACAGACCC AGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825947.1
    CDS222..7379
    Translation

    Target ORF information:

    RefSeq Version XM_012970493.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis fibronectin 1 (fn1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970493.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCGCTGGG GGGCCCTGAC CGGGCTGCTC CTAGCCCTGT GCCTGTGTGC AGTGGTACGT 
    TCCGCCCCAT CAAGCAAGAA GCGCAGGCAG GCGCAGCAGC AGCAAGTAGT GCAACCCCAT
    GGATCACCGG ACAGCCAGCA GAAAGGATGT TATGACAATG GCAAGTACTA CCAAATCAAC
    CAGCAATGGG AGCGCACCTA CCTAGGGAAC ACACTGGTGT GTACTTGTTA TGGCGGAGGC
    AGAGGTTTCA ACTGCGAAAG CAAACCAGAG TCTGAAGAGA CCTGCTTTGA CAAATACACT
    GGTGTTACTT ACCGGGTCGG TGAAACATAT GAGCGACCAA AGGATAACAT GATCTGGGAC
    TGTACGTGTA TTGGAGCTGG AAGGGGACGC ATTAGCTGTA CAATTGCAAA TCGTTGCCAT
    GAAGGAGGTC AGTCTTATAA GATTGGGGAC ACCTGGCGCA GGCCACATGA AACTGGGGGG
    TATATGCTAG AATGTGTCTG CCTTGGAAAT GGTAAAGGAG AATGGACCTG CAAGCCCGTT
    GCTGAGAGAT GCTATGATAA CACAGCTGGG ATATCCTATG TAGTTGGTCA GACCTGGGAA
    AAGCCTTATC AGGGCTGGAT GATGGTGGAC TGTACATGCT TGGGAGAAGG CAGTGGACGA
    ATCACATGTT CTTCCAAAAA CAGATGCAAT GACCAGGACA CCAGAACTTC TTATAGAATT
    GGAAATTCAT GGAGTAAGAC TGACACCAGA GGAAATCTCT TGCAATGTAT CTGTACTGGC
    AATGGGCGAG GAGAGTGGAA ATGTGATAGA CATTCCTCTG CACAAGCAAC TGGTACTGGC
    TCAAACCCCA TCACAAACAT TCAAACTACC CTCTTCCAGC CTGATTCAGA GCTGGAGCCC
    TATGGTCACT GTGTGACTGA CAATGGGGTC TTGTATTCTT TGGGTATGAG ATGGCTGAAG
    GCACAGGGCA GCAAGCAGAT GCTCTGTACC TGCCTTGGTA ATGGTGTGAG CTGTGAAGAA
    ACTGTTGAAA CCATCACATT TGGTGGTAAT GCCAATGGTG AGCCATGTGC CATTCCTTTC
    ACTCATGATG GCAAGACCTA TTATTCCTGC ACAGGAGAGG GTCGTCAGGA TGGAAAATTG
    TGGTGTGCAA CAACCTCCAA CTATGACACT GACAAGAAGT ATTCATTCTG CAATGAGCAG
    CGTGCACTTG TACAGACACG TGGTGGTAAC TCCAATGGAG CTCTTTGCAA CTTTCCATTT
    TTGTACAACA ACCGCAACTA TACAGACTGC ACCTCTGAAG GTCGGAGGGA CAGTATGAAG
    TGGTGCGGGA CTACTGCCAA CTATGATGCC GATCAAAAAT TTGGATTCTG TCCCATGGCA
    GCTCATGAGG AAATATGCAC TACTAATGAG GGAGTCATGT ATAGAGTTGG TGATCAGTGG
    GACAAGCAGC ATGATCAAGG CCACATGATG CGATGTACTT GTGTTGGAAA TGGTCGTGGG
    GAATGGACTT GTGTGGCTTA TTCTCAGCTT AAAGACCAGT GCATTGTGGA TGGGATAACT
    TATGATGTGA ACAGTTCTTT TACGAAACGC CATGAGGAAG GACACATGAT GAACTGTACC
    TGCTATGGGC AGGGTCGTGG CAGATGGAAG TGCGATGCTA TAGACCAATG CCAGGATACT
    GAAACCCGAC AGTTCTATCA GATTGGAGAT TCCTGGGAAA AGCATTTGCA GGGAGTACGG
    TACCAGTGTT ACTGCTATGG CAAAGGAATT GGTGAATGGC ATTGCCAGCC TCTATCAACA
    TCCCCAGCTG GGACTGGACC CGTTCAGGTT ATTATCACAG AGAGTTCCAA TTTCCCTAAT
    TCTCACCCTA TACAGTGGAA TGCCCCACAG CCATCACACA TTAAGAATTA CATCCTGCGC
    TGGAAGCCTA AACTGAAGAC TGGCCCATGG AAACAAGCCA CTATTCCAGG ACACCTGAAC
    TCTTATACCA TCTCTGGCTT GAAACCTGGA ATCCTGTATG AGGGGCAATT GATCAGCATT
    CTGCAGTATG GAAACAGGGA GGTCACAACC TTTGACTTTA CCACCACTTC TACCATTCAC
    CGCTCCAGTC AAACTGAGAC AGGAGAAACT ACCCCACTTC CACCATTGGT GGCCACATCT
    GAGTCTGTAA CAGAAATTAC TGCTAGCAGT TTTCTGGTAT CCTGGGTCTC TGCATCTGAC
    ACAGTGTCTG GCTTCCGTGT TGAATATGAG CTGAGTGAGG ATGGAGATGA GAAACGCTAC
    CTAGAACTTC CAAACACTGC CACTTCAGTC AGTATCCCAG ACCTCCTCCC AGGCCGTAGA
    TACAATGTGA ATGTGTATCA GATAACTGAA GAGGGTGAAA AGAGCCTCAT CCTATCAACT
    ACTCAGACAA CTGCACCTGA TGCACCCCCC GACCATAATG TGGAAAATGT TGATGACACC
    TCCATCCTGA TCAAATGGAC CAAACCTCAA GCTCCCGTCA CAGGATACAG GGTTGTATAC
    AGTCCATCTG TAGAAGGCAG CAGCACAGAG TTAAACCTCC CAAGTACTGC AACTTCAGTG
    AGCCTGACTG AGCTGCTCCC AGGAATTGAA TACAATATTA CAATCTATGC AGTGGAAGAC
    AGTTTAGAAA GTGTTCCCAT CTTTATTCAG CAAGGGACAA CTGGAACTCC ACAGACAGTT
    ATAGTACCAT CTCCCACTGA CTTACAATTA GTGGAAGTTA CCGATATAAA AATTACCATC
    ATGTGGAACC CCCCACAAAG TGAGGTTTCT GGATACCGAG TTGTTGTGAA ACCTGTAAGT
    CCCTCTGACC GTGATGTGCA GAATCTCCCC GTGAGCAGGA ACACTTTTGC CGAGGTGGTT
    AACTTGCACC CTGGCAGGAC CTATACCTTT GAGGTGTATG CAGTTAATCG TGGACAAGAG
    AGTGAACCCC TGGCTGGAGA CTTCACTACC AAACTCGATG CTCCATCAGA CTTGCAGTTT
    ACAGATGTAA CTGACTCTAC AGTTGTTATT ATCTGGAGAC CACCCCAAGC CAAAATTGGT
    CGTTACCTGC TGTCTGTAGG TCAAACCCGG GGAGGCCAGC CGAGCCAATT TCACATTAAC
    CCCTCTGCCA CAAACCACAA GCTGGAGAGT CTCTCCCCAG GCACAGAGTA CACCATTTCA
    CTTGTTGCCC TCAAAGGCAA TCAGCAAAGT GCTAGCACTA CTGGTGTCTT TTCAACATTG
    GAACCTGTGG GATCAATTCC ACCTTACAAC ACTGAAGTCA CAGAGACTAC CATAGTGGTA
    ACTTGGACCC CTGTGCCCAG AATCGGGTTT AAGCTGGATG TTCGACCCAG CCAAGGTGGG
    GAGGCACCTC GGGAAGTCAT ATCCGAGTCT GGCAGCATTG TGATCTCTGG CCTAACCCCT
    GGAGTCGAAT ATATGTACAG CATATCTGTT CTTAAGGATG GCGTTGAGAG GGACATTCCC
    ATCACCAAGA CTGTTGTCAC ACCTGTCGCT CCTCCAACCA ACTTGCGCCT CCAACCAAGC
    AGAGATTCTG CTACACTTAC TGTTTACTGG GACAGTAGCA TTTCACCAGG AATCACTGGA
    TACAGAATTA CCACTGCACC CACTCCAATG CAGGTTGGCA ATTCCCTGGA AGAGGAGGTT
    GGTCCAACTC AGACATACTG CCTCTTTGAG AACCTGAGCC CTGGTGTAGA GTACAATGTC
    AGCGTGTATG CAGTAAAAGG GGAAGAGGAG AGTTCACCTC TCTCCCAGAT CTTTCTTCAA
    GAGATACCCC AACTGACTGA CCTAAAGTAT GACGATGTAA CTGACACAAG CATTGACCTG
    AGGTGGACGC CCCTTAATTC CTCCACCATT ATCGGCTACC GAATCACGGT GGTTGCGGCG
    GGAGAATCCG TCCCTATCTA CGAGGAATTT GTGGGCCCCA CAGATGGGTA TTACAAAGTT
    TCAGGATTGG AACCCGGCAT TGACTATGAG ATCAGCGTGA TAACACTTAT TAGCGGCGGA
    GAGAGCGCCC CGACCACAAT CGTACAGCAC ACGGCTGTTC CACCTCCAAC TAATTTGCGC
    TTCACCAATG TTGGCCCTGA CACCATGCGC GTCACCTGGT CACCTCCAAC TTCTATCGAA
    CTATCAAGTT TCCTGGTACG ATATTCTCCC GTGAAGAAGC CAGATGATGT GACAGAACTC
    TCCCTCTCTC CATCTACCAA CATGGTGGTT TTATCAAACT TGCTTCCCTT CACCGAATAT
    TTAGTCAGCG TTCATTCTGT TTATGAGGAG CGAGAGAGTA GTGCCCTCAA TGGTGTAGCT
    AAGACTTACT TGGACTCTCC AACTGGCATT GACTTTTCTG AAATTACTCC CAATTCATTT
    ACTGTGCACT GGATTGCTCC ACGTGCCCCT ATCACTGGCT ACAGAATTCG GTACCAGTTA
    GAAAGTGGAG CTGGGCGACC CAAGGAGGAG CGGGTACCTC CATCCCGGAA CTCTATTACC
    CTTACCCACC TCATTCCAGG TTCAGAATAC ATTGTCAGTA TCATTGCATT CAATGGTCAA
    CAGGAGAGTT TGCCACTTTC TGGACAGCAA GCCACTGTAT CTGATGTCCC AACTGATTTG
    GAAGTGACCT CCTCCTCTCC TAACACACTC ACCATCTCTT GGGAAGCACC TTCAGTGAAT
    GTACGTTACT ACAGGATCAC CCATTCCCAG ACAGGAGGTC ATGGTCCAGA GAAGGAGTTT
    ACAGTTCCAG GAACATCCAA CACTGCAACC ATCAGAGGGT TAAATCCAGG AGTAAGCTAC
    ACCATCACTG TTTATGCAGT GACTGGGCGA GGGGATAGTC CTGCCTCTAG CAAACCACTT
    ACCATTGTTC ACATGACAGA TGTTGACCAA CCAACTGACA TGGCTGTCAC AGATATCCAG
    GATCACAGCA TTCATGTCAA ATGGTCTCCT CCTCCAGGCC CAGTCACTGG TTACAGGGTC
    ACTAGTGTCC CAAAAAGTGG CCAAGGAGAA ACCTTTTCCC AGGTCGTTTC TCCAGATCAA
    ACTGAAGTGA CGATTGTGGG CCTGCAACCA GCTGTGGAGT ATGTGGTGAG CATATACTCA
    CAAGGTGAAA ATGGCGAGAG CGAGCCTCTG GTTGAAACTG CAGTTACAAC AATTCCAGCA
    CCAACAAATC TTCAGTTCTC TCAAGTGACA CCAAGTGGCT TCTCTCTTAG CTGGCATGCA
    CCCACTATCC ATCTCAGTGG GTACCTTGTA CGTGTCAACC CTAAAGAGAA AACTGGGCCT
    ATGAAGGAAG TTAGACTTTC ACCTGGTGTG ACTGCTACAA CTGTAACTGG TCTGATGGTG
    GCCACAAAAT ATGAAGTTAA TGTGTATGCT CTTAAGGATA CCCTGACAAG TCAGCCACTG
    CAGGGTTTAA TCTCTACACT TGATAATGTA AGTCCACCTC GAAGACCTCG TGTTCAAGAT
    GTGACCGAGA CCACAGTTAC ACTGTCCTGG CGCACTAAAA CAGAGACCAT CACTGGCTTC
    CAGATTGATG CTATACCTGC CGGAGGTCAG AATCCCATCA GGAGAACAGT TGATGCAGAT
    CTCCGTTCTT TTACTATCAC AGGTCTGCAG CCTGGCACAG ACTACAAGAT CTATCTGTAC
    ACCCTAAATG ACAATGCTCG TAGCTCTCCT GTGATTGTTG ATGTCACCAC AGCTGTGGAC
    TCTCCATCCA ACCTGCGTTT CCTGACCACA ACTAGCAACT CCCTGCTGTT CACTTGGCAG
    CCACCTCGTG CTCGCATCAC TGGCTATATC ATTCGTTATG AGAAAGCTGG AGGGCTACTG
    AAAGAGCACC TCCCACGCCT CCAAGCAGGA ACCACAGAGT CTACCATTAC CAACCTGGAG
    CCAGGCACAG AATATATTGT CTATATTATT GCTGTGAGAA ACAATCAGAA GAGTGAGCCT
    CTTGTGGGAC GTAAGAGAAC AGACGAACTG CCTCGCCTGG TTACACTTCC ACACCCGGGC
    AAAGGCCCTG AAATCCTTGA TGTTCCCACT GATGAAGAGA ACACACCCCA CATCACACAA
    ATCAAGTTGG ACAATGGTAA TGGTATCCAA CTGCCAGGCT CTAATGGACA GCAGCCCAGT
    TCTGGCAATG AAGGGCAGCT TATAGAAGAA CATGGTTTCA GAAGTCCCCT TGCACCCACT
    ACTGCTGTGC CAGTGAGGCC AGGGAAATTC ACCCCTGGGC ACTACCCACA GGAGAGGGTT
    GATATTGAGC TAGACACAGA GTTCCCTGTG CAGCGTGGAG ATCTTGATGG TCCTTACCCT
    CATGGCTTAG GACCACAGCT AAATGATTCT GGCGTCCAAG AAGTGGCATC CCATACTACC
    ATTTCATGGA GACCTGAGCT GGAAACCACT GAATATATCA TCTCTTGCCA TCCTATTGAA
    CATGATGAGG CCCCATTGCA GTTTAGGGTC CCTGGAACCT CCTCTAGTGC CACATTAAAT
    GGTCTGACTC AAGGAGCCAC TTATAACATC ATTGTGGAAG CGCAGAAGGG GTCTGATAAA
    CATAAGGTCT TCGAGAAGCG TGTAACTGTT GGCAGTCCTG GTTCCCCTGA GGGGGTTTTA
    CAGCCAGCTG AGGACACCTG TTATGACACA TTCAATGGAG CACACTATTC TGTGGGACAG
    GAATGGGAAC GCATGTCAGA ATCTGGATTC AGGCTCTGGT GCAAGTGCCT GGGCTATGGA
    AGTGGACACT TCAGATGTGA CTCCTCCAAA TGGTGCCATG ATAATGGTGT CAATCACAGG
    GTCGGGGAGA AATGGGACCG ACGAGGAGAG AATGGTCAGA TGATGAGTTG TACTTGTCTT
    GGCAATGGAA AAGGAGAATT TAAGTGTGAA CCTCATGAAG CCACGTGCTA TGATGAAGGG
    AAGATGTACA ATGTTGGAGA GCAGTGGCAG AAGGAATATT TAGGGGCAAT CTGTTCATGC
    ACATGCTATG GAGGACAGCA AGGGTGGAGA TGTGACAATT GCCGCAGACC AGGTGCTGTT
    CTTCCCGATG GCACTGCTGG GCATACAGTC TCTCAGTTTG CCCAGAGATA CCAACAGAAC
    TATAATTTGA ACTGCCCCAT TGAGTGCTAC CTTCCCCTCC AAGCAGATAT ACAGCATTCG
    GAACAGACCC AGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa28345
    Clone ID Related Accession (Same CDS sequence) XM_012970494.3 , XM_012970494.2
    Accession Version XM_012970494.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product fibronectin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970494.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCGCTGGG GGGCCCTGAC CGGGCTGCTC CTAGCCCTGT GCCTGTGTGC AGTGGTACGT 
    TCCGCCCCAT CAAGCAAGAA GCGCAGGCAG GCGCAGCAGC AGCAAGTAGT GCAACCCCAT
    GGATCACCGG ACAGCCAGCA GAAAGGATGT TATGACAATG GCAAGTACTA CCAAATCAAC
    CAGCAATGGG AGCGCACCTA CCTAGGGAAC ACACTGGTGT GTACTTGTTA TGGCGGAGGC
    AGAGGTTTCA ACTGCGAAAG CAAACCAGAG TCTGAAGAGA CCTGCTTTGA CAAATACACT
    GGTGTTACTT ACCGGGTCGG TGAAACATAT GAGCGACCAA AGGATAACAT GATCTGGGAC
    TGTACGTGTA TTGGAGCTGG AAGGGGACGC ATTAGCTGTA CAATTGCAAA TCGTTGCCAT
    GAAGGAGGTC AGTCTTATAA GATTGGGGAC ACCTGGCGCA GGCCACATGA AACTGGGGGG
    TATATGCTAG AATGTGTCTG CCTTGGAAAT GGTAAAGGAG AATGGACCTG CAAGCCCGTT
    GCTGAGAGAT GCTATGATAA CACAGCTGGG ATATCCTATG TAGTTGGTCA GACCTGGGAA
    AAGCCTTATC AGGGCTGGAT GATGGTGGAC TGTACATGCT TGGGAGAAGG CAGTGGACGA
    ATCACATGTT CTTCCAAAAA CAGATGCAAT GACCAGGACA CCAGAACTTC TTATAGAATT
    GGAAATTCAT GGAGTAAGAC TGACACCAGA GGAAATCTCT TGCAATGTAT CTGTACTGGC
    AATGGGCGAG GAGAGTGGAA ATGTGATAGA CATTCCTCTG CACAAGCAAC TGGTACTGGC
    TCAAACCCCA TCACAAACAT TCAAACTACC CTCTTCCAGC CTGATTCAGA GCTGGAGCCC
    TATGGTCACT GTGTGACTGA CAATGGGGTC TTGTATTCTT TGGGTATGAG ATGGCTGAAG
    GCACAGGGCA GCAAGCAGAT GCTCTGTACC TGCCTTGGTA ATGGTGTGAG CTGTGAAGAA
    ACTGTTGAAA CCATCACATT TGGTGGTAAT GCCAATGGTG AGCCATGTGC CATTCCTTTC
    ACTCATGATG GCAAGACCTA TTATTCCTGC ACAGGAGAGG GTCGTCAGGA TGGAAAATTG
    TGGTGTGCAA CAACCTCCAA CTATGACACT GACAAGAAGT ATTCATTCTG CAATGAGCAG
    CGTGCACTTG TACAGACACG TGGTGGTAAC TCCAATGGAG CTCTTTGCAA CTTTCCATTT
    TTGTACAACA ACCGCAACTA TACAGACTGC ACCTCTGAAG GTCGGAGGGA CAGTATGAAG
    TGGTGCGGGA CTACTGCCAA CTATGATGCC GATCAAAAAT TTGGATTCTG TCCCATGGCA
    GCTCATGAGG AAATATGCAC TACTAATGAG GGAGTCATGT ATAGAGTTGG TGATCAGTGG
    GACAAGCAGC ATGATCAAGG CCACATGATG CGATGTACTT GTGTTGGAAA TGGTCGTGGG
    GAATGGACTT GTGTGGCTTA TTCTCAGCTT AAAGACCAGT GCATTGTGGA TGGGATAACT
    TATGATGTGA ACAGTTCTTT TACGAAACGC CATGAGGAAG GACACATGAT GAACTGTACC
    TGCTATGGGC AGGGTCGTGG CAGATGGAAG TGCGATGCTA TAGACCAATG CCAGGATACT
    GAAACCCGAC AGTTCTATCA GATTGGAGAT TCCTGGGAAA AGCATTTGCA GGGAGTACGG
    TACCAGTGTT ACTGCTATGG CAAAGGAATT GGTGAATGGC ATTGCCAGCC TCTATCAACA
    TCCCCAGCTG GGACTGGACC CGTTCAGGTT ATTATCACAG AGAGTTCCAA TTTCCCTAAT
    TCTCACCCTA TACAGTGGAA TGCCCCACAG CCATCACACA TTAAGAATTA CATCCTGCGC
    TGGAAGCCTA AACTGAAGAC TGGCCCATGG AAACAAGCCA CTATTCCAGG ACACCTGAAC
    TCTTATACCA TCTCTGGCTT GAAACCTGGA ATCCTGTATG AGGGGCAATT GATCAGCATT
    CTGCAGTATG GAAACAGGGA GGTCACAACC TTTGACTTTA CCACCACTTC TACCATTCAC
    CGCTCCAGTC AAACTGAGAC AGGAGAAACT ACCCCACTTC CACCATTGGT GGCCACATCT
    GAGTCTGTAA CAGAAATTAC TGCTAGCAGT TTTCTGGTAT CCTGGGTCTC TGCATCTGAC
    ACAGTGTCTG GCTTCCGTGT TGAATATGAG CTGAGTGAGG ATGGAGATGA GAAACGCTAC
    CTAGAACTTC CAAACACTGC CACTTCAGTC AGTATCCCAG ACCTCCTCCC AGGCCGTAGA
    TACAATGTGA ATGTGTATCA GATAACTGAA GAGGGTGAAA AGAGCCTCAT CCTATCAACT
    ACTCAGACAA CTGCACCTGA TGCACCCCCC GACCATAATG TGGAAAATGT TGATGACACC
    TCCATCCTGA TCAAATGGAC CAAACCTCAA GCTCCCGTCA CAGGATACAG GGTTGTATAC
    AGTCCATCTG TAGAAGGCAG CAGCACAGAG TTAAACCTCC CAAGTACTGC AACTTCAGTG
    AGCCTGACTG AGCTGCTCCC AGGAATTGAA TACAATATTA CAATCTATGC AGTGGAAGAC
    AGTTTAGAAA GTGTTCCCAT CTTTATTCAG CAAGGGACAA CTGGAACTCC ACAGACAGTT
    ATAGTACCAT CTCCCACTGA CTTACAATTA GTGGAAGTTA CCGATATAAA AATTACCATC
    ATGTGGAACC CCCCACAAAG TGAGGTTTCT GGATACCGAG TTGTTGTGAA ACCTGTAAGT
    CCCTCTGACC GTGATGTGCA GAATCTCCCC GTGAGCAGGA ACACTTTTGC CGAGGTGGTT
    AACTTGCACC CTGGCAGGAC CTATACCTTT GAGGTGTATG CAGTTAATCG TGGACAAGAG
    AGTGAACCCC TGGCTGGAGA CTTCACTACC AAACTCGATG CTCCATCAGA CTTGCAGTTT
    ACAGATGTAA CTGACTCTAC AGTTGTTATT ATCTGGAGAC CACCCCAAGC CAAAATTGGT
    CGTTACCTGC TGTCTGTAGG TCAAACCCGG GGAGGCCAGC CGAGCCAATT TCACATTAAC
    CCCTCTGCCA CAAACCACAA GCTGGAGAGT CTCTCCCCAG GCACAGAGTA CACCATTTCA
    CTTGTTGCCC TCAAAGGCAA TCAGCAAAGT GCTAGCACTA CTGGTGTCTT TTCAACATTG
    GAACCTGTGG GATCAATTCC ACCTTACAAC ACTGAAGTCA CAGAGACTAC CATAGTGGTA
    ACTTGGACCC CTGTGCCCAG AATCGGGTTT AAGCTGGATG TTCGACCCAG CCAAGGTGGG
    GAGGCACCTC GGGAAGTCAT ATCCGAGTCT GGCAGCATTG TGATCTCTGG CCTAACCCCT
    GGAGTCGAAT ATATGTACAG CATATCTGTT CTTAAGGATG GCGTTGAGAG GGACATTCCC
    ATCACCAAGA CTGTTGTCAC ACCTGTCGCT CCTCCAACCA ACTTGCGCCT CCAACCAAGC
    AGAGATTCTG CTACACTTAC TGTTTACTGG GACAGTAGCA TTTCACCAGG AATCACTGGA
    TACAGAATTA CCACTGCACC CACTCCAATG CAGGTTGGCA ATTCCCTGGA AGAGGAGGTT
    GGTCCAACTC AGACATACTG CCTCTTTGAG AACCTGAGCC CTGGTGTAGA GTACAATGTC
    AGCGTGTATG CAGTAAAAGG GGAAGAGGAG AGTTCACCTC TCTCCCAGAT CTTTCTTCAA
    GCTGTTCCAC CTCCAACTAA TTTGCGCTTC ACCAATGTTG GCCCTGACAC CATGCGCGTC
    ACCTGGTCAC CTCCAACTTC TATCGAACTA TCAAGTTTCC TGGTACGATA TTCTCCCGTG
    AAGAAGCCAG ATGATGTGAC AGAACTCTCC CTCTCTCCAT CTACCAACAT GGTGGTTTTA
    TCAAACTTGC TTCCCTTCAC CGAATATTTA GTCAGCGTTC ATTCTGTTTA TGAGGAGCGA
    GAGAGTAGTG CCCTCAATGG TGTAGCTAAG ACTTACTTGG ACTCTCCAAC TGGCATTGAC
    TTTTCTGAAA TTACTCCCAA TTCATTTACT GTGCACTGGA TTGCTCCACG TGCCCCTATC
    ACTGGCTACA GAATTCGGTA CCAGTTAGAA AGTGGAGCTG GGCGACCCAA GGAGGAGCGG
    GTACCTCCAT CCCGGAACTC TATTACCCTT ACCCACCTCA TTCCAGGTTC AGAATACATT
    GTCAGTATCA TTGCATTCAA TGGTCAACAG GAGAGTTTGC CACTTTCTGG ACAGCAAGCC
    ACTGTATCTG ATGTCCCAAC TGATTTGGAA GTGACCTCCT CCTCTCCTAA CACACTCACC
    ATCTCTTGGG AAGCACCTTC AGTGAATGTA CGTTACTACA GGATCACCCA TTCCCAGACA
    GGAGGTCATG GTCCAGAGAA GGAGTTTACA GTTCCAGGAA CATCCAACAC TGCAACCATC
    AGAGGGTTAA ATCCAGGAGT AAGCTACACC ATCACTGTTT ATGCAGTGAC TGGGCGAGGG
    GATAGTCCTG CCTCTAGCAA ACCACTTACC ATTGTTCACA TGACAGATGT TGACCAACCA
    ACTGACATGG CTGTCACAGA TATCCAGGAT CACAGCATTC ATGTCAAATG GTCTCCTCCT
    CCAGGCCCAG TCACTGGTTA CAGGGTCACT AGTGTCCCAA AAAGTGGCCA AGGAGAAACC
    TTTTCCCAGG TCGTTTCTCC AGATCAAACT GAAGTGACGA TTGTGGGCCT GCAACCAGCT
    GTGGAGTATG TGGTGAGCAT ATACTCACAA GGTGAAAATG GCGAGAGCGA GCCTCTGGTT
    GAAACTGCAG TTACAAACAT TGACCATCCC AAAGGACTGA CATTCACTGA TGTGGGTGTT
    GATTCCATTA GACTGGCTTG GGAGGTCCCA GATGGGCAGG TCACCCGCTA CAGGGTTACC
    TACTCAAGTC CTGAGGAGGG AGTAAAAGAG TTGTTCCCAG CTCCAGAAGG GGATGATGAC
    ACAGCAGAAT TGCACGGTCT GAGGCCGGGC ACAGAATACA CTGTGAGCAT CGTGGCATTG
    CACGATGACA TGGAGAGCAA GCCGATGATT GGAATCCAGA GCACAGCAAT TCCAGCACCA
    ACAAATCTTC AGTTCTCTCA AGTGACACCA AGTGGCTTCT CTCTTAGCTG GCATGCACCC
    ACTATCCATC TCAGTGGGTA CCTTGTACGT GTCAACCCTA AAGAGAAAAC TGGGCCTATG
    AAGGAAGTTA GACTTTCACC TGGTGTGACT GCTACAACTG TAACTGGTCT GATGGTGGCC
    ACAAAATATG AAGTTAATGT GTATGCTCTT AAGGATACCC TGACAAGTCA GCCACTGCAG
    GGTTTAATCT CTACACTTGA TAATGTAAGT CCACCTCGAA GACCTCGTGT TCAAGATGTG
    ACCGAGACCA CAGTTACACT GTCCTGGCGC ACTAAAACAG AGACCATCAC TGGCTTCCAG
    ATTGATGCTA TACCTGCCGG AGGTCAGAAT CCCATCAGGA GAACAGTTGA TGCAGATCTC
    CGTTCTTTTA CTATCACAGG TCTGCAGCCT GGCACAGACT ACAAGATCTA TCTGTACACC
    CTAAATGACA ATGCTCGTAG CTCTCCTGTG ATTGTTGATG TCACCACAGC TGTGGACTCT
    CCATCCAACC TGCGTTTCCT GACCACAACT AGCAACTCCC TGCTGTTCAC TTGGCAGCCA
    CCTCGTGCTC GCATCACTGG CTATATCATT CGTTATGAGA AAGCTGGAGG GCTACTGAAA
    GAGCACCTCC CACGCCTCCA AGCAGGAACC ACAGAGTCTA CCATTACCAA CCTGGAGCCA
    GGCACAGAAT ATATTGTCTA TATTATTGCT GTGAGAAACA ATCAGAAGAG TGAGCCTCTT
    GTGGGACGTA AGAGAACAGA CGAACTGCCT CGCCTGGTTA CACTTCCACA CCCGGGCAAA
    GGCCCTGAAA TCCTTGATGT TCCCACTGAT GAAGAGAACA CACCCCACAT CACACAAATC
    AAGTTGGACA ATGGTAATGG TATCCAACTG CCAGGCTCTA ATGGACAGCA GCCCAGTTCT
    GGCAATGAAG GGCAGCTTAT AGAAGAACAT GGTTTCAGAA GTCCCCTTGC ACCCACTACT
    GCTGTGCCAG TGAGGCCAGG GAAATTCACC CCTGGGCACT ACCCACAGGA GAGGGTTGAT
    ATTGAGCTAG ACACAGAGTT CCCTGTGCAG CGTGGAGATC TTGATGGTCC TTACCCTCAT
    GGCTTAGGAC CACAGCTAAA TGATTCTGGC GTCCAAGAAG TGGCATCCCA TACTACCATT
    TCATGGAGAC CTGAGCTGGA AACCACTGAA TATATCATCT CTTGCCATCC TATTGAACAT
    GATGAGGCCC CATTGCAGTT TAGGGTCCCT GGAACCTCCT CTAGTGCCAC ATTAAATGGT
    CTGACTCAAG GAGCCACTTA TAACATCATT GTGGAAGCGC AGAAGGGGTC TGATAAACAT
    AAGGTCTTCG AGAAGCGTGT AACTGTTGGC AGTCCTGGTT CCCCTGAGGG GGTTTTACAG
    CCAGCTGAGG ACACCTGTTA TGACACATTC AATGGAGCAC ACTATTCTGT GGGACAGGAA
    TGGGAACGCA TGTCAGAATC TGGATTCAGG CTCTGGTGCA AGTGCCTGGG CTATGGAAGT
    GGACACTTCA GATGTGACTC CTCCAAATGG TGCCATGATA ATGGTGTCAA TCACAGGGTC
    GGGGAGAAAT GGGACCGACG AGGAGAGAAT GGTCAGATGA TGAGTTGTAC TTGTCTTGGC
    AATGGAAAAG GAGAATTTAA GTGTGAACCT CATGAAGCCA CGTGCTATGA TGAAGGGAAG
    ATGTACAATG TTGGAGAGCA GTGGCAGAAG GAATATTTAG GGGCAATCTG TTCATGCACA
    TGCTATGGAG GACAGCAAGG GTGGAGATGT GACAATTGCC GCAGACCAGG TGCTGTTCTT
    CCCGATGGCA CTGCTGGGCA TACAGTCTCT CAGTTTGCCC AGAGATACCA ACAGAACTAT
    AATTTGAACT GCCCCATTGA GTGCTACCTT CCCCTCCAAG CAGATATACA GCATTCGGAA
    CAGACCCAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825948.1
    CDS222..7376
    Translation

    Target ORF information:

    RefSeq Version XM_012970494.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis fibronectin 1 (fn1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970494.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCGCTGGG GGGCCCTGAC CGGGCTGCTC CTAGCCCTGT GCCTGTGTGC AGTGGTACGT 
    TCCGCCCCAT CAAGCAAGAA GCGCAGGCAG GCGCAGCAGC AGCAAGTAGT GCAACCCCAT
    GGATCACCGG ACAGCCAGCA GAAAGGATGT TATGACAATG GCAAGTACTA CCAAATCAAC
    CAGCAATGGG AGCGCACCTA CCTAGGGAAC ACACTGGTGT GTACTTGTTA TGGCGGAGGC
    AGAGGTTTCA ACTGCGAAAG CAAACCAGAG TCTGAAGAGA CCTGCTTTGA CAAATACACT
    GGTGTTACTT ACCGGGTCGG TGAAACATAT GAGCGACCAA AGGATAACAT GATCTGGGAC
    TGTACGTGTA TTGGAGCTGG AAGGGGACGC ATTAGCTGTA CAATTGCAAA TCGTTGCCAT
    GAAGGAGGTC AGTCTTATAA GATTGGGGAC ACCTGGCGCA GGCCACATGA AACTGGGGGG
    TATATGCTAG AATGTGTCTG CCTTGGAAAT GGTAAAGGAG AATGGACCTG CAAGCCCGTT
    GCTGAGAGAT GCTATGATAA CACAGCTGGG ATATCCTATG TAGTTGGTCA GACCTGGGAA
    AAGCCTTATC AGGGCTGGAT GATGGTGGAC TGTACATGCT TGGGAGAAGG CAGTGGACGA
    ATCACATGTT CTTCCAAAAA CAGATGCAAT GACCAGGACA CCAGAACTTC TTATAGAATT
    GGAAATTCAT GGAGTAAGAC TGACACCAGA GGAAATCTCT TGCAATGTAT CTGTACTGGC
    AATGGGCGAG GAGAGTGGAA ATGTGATAGA CATTCCTCTG CACAAGCAAC TGGTACTGGC
    TCAAACCCCA TCACAAACAT TCAAACTACC CTCTTCCAGC CTGATTCAGA GCTGGAGCCC
    TATGGTCACT GTGTGACTGA CAATGGGGTC TTGTATTCTT TGGGTATGAG ATGGCTGAAG
    GCACAGGGCA GCAAGCAGAT GCTCTGTACC TGCCTTGGTA ATGGTGTGAG CTGTGAAGAA
    ACTGTTGAAA CCATCACATT TGGTGGTAAT GCCAATGGTG AGCCATGTGC CATTCCTTTC
    ACTCATGATG GCAAGACCTA TTATTCCTGC ACAGGAGAGG GTCGTCAGGA TGGAAAATTG
    TGGTGTGCAA CAACCTCCAA CTATGACACT GACAAGAAGT ATTCATTCTG CAATGAGCAG
    CGTGCACTTG TACAGACACG TGGTGGTAAC TCCAATGGAG CTCTTTGCAA CTTTCCATTT
    TTGTACAACA ACCGCAACTA TACAGACTGC ACCTCTGAAG GTCGGAGGGA CAGTATGAAG
    TGGTGCGGGA CTACTGCCAA CTATGATGCC GATCAAAAAT TTGGATTCTG TCCCATGGCA
    GCTCATGAGG AAATATGCAC TACTAATGAG GGAGTCATGT ATAGAGTTGG TGATCAGTGG
    GACAAGCAGC ATGATCAAGG CCACATGATG CGATGTACTT GTGTTGGAAA TGGTCGTGGG
    GAATGGACTT GTGTGGCTTA TTCTCAGCTT AAAGACCAGT GCATTGTGGA TGGGATAACT
    TATGATGTGA ACAGTTCTTT TACGAAACGC CATGAGGAAG GACACATGAT GAACTGTACC
    TGCTATGGGC AGGGTCGTGG CAGATGGAAG TGCGATGCTA TAGACCAATG CCAGGATACT
    GAAACCCGAC AGTTCTATCA GATTGGAGAT TCCTGGGAAA AGCATTTGCA GGGAGTACGG
    TACCAGTGTT ACTGCTATGG CAAAGGAATT GGTGAATGGC ATTGCCAGCC TCTATCAACA
    TCCCCAGCTG GGACTGGACC CGTTCAGGTT ATTATCACAG AGAGTTCCAA TTTCCCTAAT
    TCTCACCCTA TACAGTGGAA TGCCCCACAG CCATCACACA TTAAGAATTA CATCCTGCGC
    TGGAAGCCTA AACTGAAGAC TGGCCCATGG AAACAAGCCA CTATTCCAGG ACACCTGAAC
    TCTTATACCA TCTCTGGCTT GAAACCTGGA ATCCTGTATG AGGGGCAATT GATCAGCATT
    CTGCAGTATG GAAACAGGGA GGTCACAACC TTTGACTTTA CCACCACTTC TACCATTCAC
    CGCTCCAGTC AAACTGAGAC AGGAGAAACT ACCCCACTTC CACCATTGGT GGCCACATCT
    GAGTCTGTAA CAGAAATTAC TGCTAGCAGT TTTCTGGTAT CCTGGGTCTC TGCATCTGAC
    ACAGTGTCTG GCTTCCGTGT TGAATATGAG CTGAGTGAGG ATGGAGATGA GAAACGCTAC
    CTAGAACTTC CAAACACTGC CACTTCAGTC AGTATCCCAG ACCTCCTCCC AGGCCGTAGA
    TACAATGTGA ATGTGTATCA GATAACTGAA GAGGGTGAAA AGAGCCTCAT CCTATCAACT
    ACTCAGACAA CTGCACCTGA TGCACCCCCC GACCATAATG TGGAAAATGT TGATGACACC
    TCCATCCTGA TCAAATGGAC CAAACCTCAA GCTCCCGTCA CAGGATACAG GGTTGTATAC
    AGTCCATCTG TAGAAGGCAG CAGCACAGAG TTAAACCTCC CAAGTACTGC AACTTCAGTG
    AGCCTGACTG AGCTGCTCCC AGGAATTGAA TACAATATTA CAATCTATGC AGTGGAAGAC
    AGTTTAGAAA GTGTTCCCAT CTTTATTCAG CAAGGGACAA CTGGAACTCC ACAGACAGTT
    ATAGTACCAT CTCCCACTGA CTTACAATTA GTGGAAGTTA CCGATATAAA AATTACCATC
    ATGTGGAACC CCCCACAAAG TGAGGTTTCT GGATACCGAG TTGTTGTGAA ACCTGTAAGT
    CCCTCTGACC GTGATGTGCA GAATCTCCCC GTGAGCAGGA ACACTTTTGC CGAGGTGGTT
    AACTTGCACC CTGGCAGGAC CTATACCTTT GAGGTGTATG CAGTTAATCG TGGACAAGAG
    AGTGAACCCC TGGCTGGAGA CTTCACTACC AAACTCGATG CTCCATCAGA CTTGCAGTTT
    ACAGATGTAA CTGACTCTAC AGTTGTTATT ATCTGGAGAC CACCCCAAGC CAAAATTGGT
    CGTTACCTGC TGTCTGTAGG TCAAACCCGG GGAGGCCAGC CGAGCCAATT TCACATTAAC
    CCCTCTGCCA CAAACCACAA GCTGGAGAGT CTCTCCCCAG GCACAGAGTA CACCATTTCA
    CTTGTTGCCC TCAAAGGCAA TCAGCAAAGT GCTAGCACTA CTGGTGTCTT TTCAACATTG
    GAACCTGTGG GATCAATTCC ACCTTACAAC ACTGAAGTCA CAGAGACTAC CATAGTGGTA
    ACTTGGACCC CTGTGCCCAG AATCGGGTTT AAGCTGGATG TTCGACCCAG CCAAGGTGGG
    GAGGCACCTC GGGAAGTCAT ATCCGAGTCT GGCAGCATTG TGATCTCTGG CCTAACCCCT
    GGAGTCGAAT ATATGTACAG CATATCTGTT CTTAAGGATG GCGTTGAGAG GGACATTCCC
    ATCACCAAGA CTGTTGTCAC ACCTGTCGCT CCTCCAACCA ACTTGCGCCT CCAACCAAGC
    AGAGATTCTG CTACACTTAC TGTTTACTGG GACAGTAGCA TTTCACCAGG AATCACTGGA
    TACAGAATTA CCACTGCACC CACTCCAATG CAGGTTGGCA ATTCCCTGGA AGAGGAGGTT
    GGTCCAACTC AGACATACTG CCTCTTTGAG AACCTGAGCC CTGGTGTAGA GTACAATGTC
    AGCGTGTATG CAGTAAAAGG GGAAGAGGAG AGTTCACCTC TCTCCCAGAT CTTTCTTCAA
    GCTGTTCCAC CTCCAACTAA TTTGCGCTTC ACCAATGTTG GCCCTGACAC CATGCGCGTC
    ACCTGGTCAC CTCCAACTTC TATCGAACTA TCAAGTTTCC TGGTACGATA TTCTCCCGTG
    AAGAAGCCAG ATGATGTGAC AGAACTCTCC CTCTCTCCAT CTACCAACAT GGTGGTTTTA
    TCAAACTTGC TTCCCTTCAC CGAATATTTA GTCAGCGTTC ATTCTGTTTA TGAGGAGCGA
    GAGAGTAGTG CCCTCAATGG TGTAGCTAAG ACTTACTTGG ACTCTCCAAC TGGCATTGAC
    TTTTCTGAAA TTACTCCCAA TTCATTTACT GTGCACTGGA TTGCTCCACG TGCCCCTATC
    ACTGGCTACA GAATTCGGTA CCAGTTAGAA AGTGGAGCTG GGCGACCCAA GGAGGAGCGG
    GTACCTCCAT CCCGGAACTC TATTACCCTT ACCCACCTCA TTCCAGGTTC AGAATACATT
    GTCAGTATCA TTGCATTCAA TGGTCAACAG GAGAGTTTGC CACTTTCTGG ACAGCAAGCC
    ACTGTATCTG ATGTCCCAAC TGATTTGGAA GTGACCTCCT CCTCTCCTAA CACACTCACC
    ATCTCTTGGG AAGCACCTTC AGTGAATGTA CGTTACTACA GGATCACCCA TTCCCAGACA
    GGAGGTCATG GTCCAGAGAA GGAGTTTACA GTTCCAGGAA CATCCAACAC TGCAACCATC
    AGAGGGTTAA ATCCAGGAGT AAGCTACACC ATCACTGTTT ATGCAGTGAC TGGGCGAGGG
    GATAGTCCTG CCTCTAGCAA ACCACTTACC ATTGTTCACA TGACAGATGT TGACCAACCA
    ACTGACATGG CTGTCACAGA TATCCAGGAT CACAGCATTC ATGTCAAATG GTCTCCTCCT
    CCAGGCCCAG TCACTGGTTA CAGGGTCACT AGTGTCCCAA AAAGTGGCCA AGGAGAAACC
    TTTTCCCAGG TCGTTTCTCC AGATCAAACT GAAGTGACGA TTGTGGGCCT GCAACCAGCT
    GTGGAGTATG TGGTGAGCAT ATACTCACAA GGTGAAAATG GCGAGAGCGA GCCTCTGGTT
    GAAACTGCAG TTACAAACAT TGACCATCCC AAAGGACTGA CATTCACTGA TGTGGGTGTT
    GATTCCATTA GACTGGCTTG GGAGGTCCCA GATGGGCAGG TCACCCGCTA CAGGGTTACC
    TACTCAAGTC CTGAGGAGGG AGTAAAAGAG TTGTTCCCAG CTCCAGAAGG GGATGATGAC
    ACAGCAGAAT TGCACGGTCT GAGGCCGGGC ACAGAATACA CTGTGAGCAT CGTGGCATTG
    CACGATGACA TGGAGAGCAA GCCGATGATT GGAATCCAGA GCACAGCAAT TCCAGCACCA
    ACAAATCTTC AGTTCTCTCA AGTGACACCA AGTGGCTTCT CTCTTAGCTG GCATGCACCC
    ACTATCCATC TCAGTGGGTA CCTTGTACGT GTCAACCCTA AAGAGAAAAC TGGGCCTATG
    AAGGAAGTTA GACTTTCACC TGGTGTGACT GCTACAACTG TAACTGGTCT GATGGTGGCC
    ACAAAATATG AAGTTAATGT GTATGCTCTT AAGGATACCC TGACAAGTCA GCCACTGCAG
    GGTTTAATCT CTACACTTGA TAATGTAAGT CCACCTCGAA GACCTCGTGT TCAAGATGTG
    ACCGAGACCA CAGTTACACT GTCCTGGCGC ACTAAAACAG AGACCATCAC TGGCTTCCAG
    ATTGATGCTA TACCTGCCGG AGGTCAGAAT CCCATCAGGA GAACAGTTGA TGCAGATCTC
    CGTTCTTTTA CTATCACAGG TCTGCAGCCT GGCACAGACT ACAAGATCTA TCTGTACACC
    CTAAATGACA ATGCTCGTAG CTCTCCTGTG ATTGTTGATG TCACCACAGC TGTGGACTCT
    CCATCCAACC TGCGTTTCCT GACCACAACT AGCAACTCCC TGCTGTTCAC TTGGCAGCCA
    CCTCGTGCTC GCATCACTGG CTATATCATT CGTTATGAGA AAGCTGGAGG GCTACTGAAA
    GAGCACCTCC CACGCCTCCA AGCAGGAACC ACAGAGTCTA CCATTACCAA CCTGGAGCCA
    GGCACAGAAT ATATTGTCTA TATTATTGCT GTGAGAAACA ATCAGAAGAG TGAGCCTCTT
    GTGGGACGTA AGAGAACAGA CGAACTGCCT CGCCTGGTTA CACTTCCACA CCCGGGCAAA
    GGCCCTGAAA TCCTTGATGT TCCCACTGAT GAAGAGAACA CACCCCACAT CACACAAATC
    AAGTTGGACA ATGGTAATGG TATCCAACTG CCAGGCTCTA ATGGACAGCA GCCCAGTTCT
    GGCAATGAAG GGCAGCTTAT AGAAGAACAT GGTTTCAGAA GTCCCCTTGC ACCCACTACT
    GCTGTGCCAG TGAGGCCAGG GAAATTCACC CCTGGGCACT ACCCACAGGA GAGGGTTGAT
    ATTGAGCTAG ACACAGAGTT CCCTGTGCAG CGTGGAGATC TTGATGGTCC TTACCCTCAT
    GGCTTAGGAC CACAGCTAAA TGATTCTGGC GTCCAAGAAG TGGCATCCCA TACTACCATT
    TCATGGAGAC CTGAGCTGGA AACCACTGAA TATATCATCT CTTGCCATCC TATTGAACAT
    GATGAGGCCC CATTGCAGTT TAGGGTCCCT GGAACCTCCT CTAGTGCCAC ATTAAATGGT
    CTGACTCAAG GAGCCACTTA TAACATCATT GTGGAAGCGC AGAAGGGGTC TGATAAACAT
    AAGGTCTTCG AGAAGCGTGT AACTGTTGGC AGTCCTGGTT CCCCTGAGGG GGTTTTACAG
    CCAGCTGAGG ACACCTGTTA TGACACATTC AATGGAGCAC ACTATTCTGT GGGACAGGAA
    TGGGAACGCA TGTCAGAATC TGGATTCAGG CTCTGGTGCA AGTGCCTGGG CTATGGAAGT
    GGACACTTCA GATGTGACTC CTCCAAATGG TGCCATGATA ATGGTGTCAA TCACAGGGTC
    GGGGAGAAAT GGGACCGACG AGGAGAGAAT GGTCAGATGA TGAGTTGTAC TTGTCTTGGC
    AATGGAAAAG GAGAATTTAA GTGTGAACCT CATGAAGCCA CGTGCTATGA TGAAGGGAAG
    ATGTACAATG TTGGAGAGCA GTGGCAGAAG GAATATTTAG GGGCAATCTG TTCATGCACA
    TGCTATGGAG GACAGCAAGG GTGGAGATGT GACAATTGCC GCAGACCAGG TGCTGTTCTT
    CCCGATGGCA CTGCTGGGCA TACAGTCTCT CAGTTTGCCC AGAGATACCA ACAGAACTAT
    AATTTGAACT GCCCCATTGA GTGCTACCTT CCCCTCCAAG CAGATATACA GCATTCGGAA
    CAGACCCAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa28346
    Clone ID Related Accession (Same CDS sequence) XM_012970495.3 , XM_012970495.2
    Accession Version XM_012970495.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product fibronectin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970495.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCGCTGGG GGGCCCTGAC CGGGCTGCTC CTAGCCCTGT GCCTGTGTGC AGTGGTACGT 
    TCCGCCCCAT CAAGCAAGAA GCGCAGGCAG GCGCAGCAGC AGCAAGTAGT GCAACCCCAT
    GGATCACCGG ACAGCCAGCA GAAAGGATGT TATGACAATG GCAAGTACTA CCAAATCAAC
    CAGCAATGGG AGCGCACCTA CCTAGGGAAC ACACTGGTGT GTACTTGTTA TGGCGGAGGC
    AGAGGTTTCA ACTGCGAAAG CAAACCAGAG TCTGAAGAGA CCTGCTTTGA CAAATACACT
    GGTGTTACTT ACCGGGTCGG TGAAACATAT GAGCGACCAA AGGATAACAT GATCTGGGAC
    TGTACGTGTA TTGGAGCTGG AAGGGGACGC ATTAGCTGTA CAATTGCAAA TCGTTGCCAT
    GAAGGAGGTC AGTCTTATAA GATTGGGGAC ACCTGGCGCA GGCCACATGA AACTGGGGGG
    TATATGCTAG AATGTGTCTG CCTTGGAAAT GGTAAAGGAG AATGGACCTG CAAGCCCGTT
    GCTGAGAGAT GCTATGATAA CACAGCTGGG ATATCCTATG TAGTTGGTCA GACCTGGGAA
    AAGCCTTATC AGGGCTGGAT GATGGTGGAC TGTACATGCT TGGGAGAAGG CAGTGGACGA
    ATCACATGTT CTTCCAAAAA CAGATGCAAT GACCAGGACA CCAGAACTTC TTATAGAATT
    GGAAATTCAT GGAGTAAGAC TGACACCAGA GGAAATCTCT TGCAATGTAT CTGTACTGGC
    AATGGGCGAG GAGAGTGGAA ATGTGATAGA CATTCCTCTG CACAAGCAAC TGGTACTGGC
    TCAAACCCCA TCACAAACAT TCAAACTACC CTCTTCCAGC CTGATTCAGA GCTGGAGCCC
    TATGGTCACT GTGTGACTGA CAATGGGGTC TTGTATTCTT TGGGTATGAG ATGGCTGAAG
    GCACAGGGCA GCAAGCAGAT GCTCTGTACC TGCCTTGGTA ATGGTGTGAG CTGTGAAGAA
    ACTGTTGAAA CCATCACATT TGGTGGTAAT GCCAATGGTG AGCCATGTGC CATTCCTTTC
    ACTCATGATG GCAAGACCTA TTATTCCTGC ACAGGAGAGG GTCGTCAGGA TGGAAAATTG
    TGGTGTGCAA CAACCTCCAA CTATGACACT GACAAGAAGT ATTCATTCTG CAATGAGCAG
    CGTGCACTTG TACAGACACG TGGTGGTAAC TCCAATGGAG CTCTTTGCAA CTTTCCATTT
    TTGTACAACA ACCGCAACTA TACAGACTGC ACCTCTGAAG GTCGGAGGGA CAGTATGAAG
    TGGTGCGGGA CTACTGCCAA CTATGATGCC GATCAAAAAT TTGGATTCTG TCCCATGGCA
    GCTCATGAGG AAATATGCAC TACTAATGAG GGAGTCATGT ATAGAGTTGG TGATCAGTGG
    GACAAGCAGC ATGATCAAGG CCACATGATG CGATGTACTT GTGTTGGAAA TGGTCGTGGG
    GAATGGACTT GTGTGGCTTA TTCTCAGCTT AAAGACCAGT GCATTGTGGA TGGGATAACT
    TATGATGTGA ACAGTTCTTT TACGAAACGC CATGAGGAAG GACACATGAT GAACTGTACC
    TGCTATGGGC AGGGTCGTGG CAGATGGAAG TGCGATGCTA TAGACCAATG CCAGGATACT
    GAAACCCGAC AGTTCTATCA GATTGGAGAT TCCTGGGAAA AGCATTTGCA GGGAGTACGG
    TACCAGTGTT ACTGCTATGG CAAAGGAATT GGTGAATGGC ATTGCCAGCC TCTATCAACA
    TCCCCAGCTG GGACTGGACC CGTTCAGGTT ATTATCACAG AGAGTTCCAA TTTCCCTAAT
    TCTCACCCTA TACAGTGGAA TGCCCCACAG CCATCACACA TTAAGAATTA CATCCTGCGC
    TGGAAGCCTA AACTGAAGAC TGGCCCATGG AAACAAGCCA CTATTCCAGG ACACCTGAAC
    TCTTATACCA TCTCTGGCTT GAAACCTGGA ATCCTGTATG AGGGGCAATT GATCAGCATT
    CTGCAGTATG GAAACAGGGA GGTCACAACC TTTGACTTTA CCACCACTTC TACCATTCAC
    CGCTCCAGTC AAACTGAGAC AGGAGAAACT ACCCCACTTC CACCATTGGT GGCCACATCT
    GAGTCTGTAA CAGAAATTAC TGCTAGCAGT TTTCTGGTAT CCTGGGTCTC TGCATCTGAC
    ACAGTGTCTG GCTTCCGTGT TGAATATGAG CTGAGTGAGG ATGGAGATGA GAAACGCTAC
    CTAGAACTTC CAAACACTGC CACTTCAGTC AGTATCCCAG ACCTCCTCCC AGGCCGTAGA
    TACAATGTGA ATGTGTATCA GATAACTGAA GAGGGTGAAA AGAGCCTCAT CCTATCAACT
    ACTCAGACAA CTGCACCTGA TGCACCCCCC GACCATAATG TGGAAAATGT TGATGACACC
    TCCATCCTGA TCAAATGGAC CAAACCTCAA GCTCCCGTCA CAGGATACAG GGTTGTATAC
    AGTCCATCTG TAGAAGGCAG CAGCACAGAG TTAAACCTCC CAAGTACTGC AACTTCAGTG
    AGCCTGACTG AGCTGCTCCC AGGAATTGAA TACAATATTA CAATCTATGC AGTGGAAGAC
    AGTTTAGAAA GTGTTCCCAT CTTTATTCAG CAAGGGACAA CTGGAACTCC ACAGACAGTT
    ATAGTACCAT CTCCCACTGA CTTACAATTA GTGGAAGTTA CCGATATAAA AATTACCATC
    ATGTGGAACC CCCCACAAAG TGAGGTTTCT GGATACCGAG TTGTTGTGAA ACCTGTAAGT
    CCCTCTGACC GTGATGTGCA GAATCTCCCC GTGAGCAGGA ACACTTTTGC CGAGGTGGTT
    AACTTGCACC CTGGCAGGAC CTATACCTTT GAGGTGTATG CAGTTAATCG TGGACAAGAG
    AGTGAACCCC TGGCTGGAGA CTTCACTACC AAACTCGATG CTCCATCAGA CTTGCAGTTT
    ACAGATGTAA CTGACTCTAC AGTTGTTATT ATCTGGAGAC CACCCCAAGC CAAAATTGGT
    CGTTACCTGC TGTCTGTAGG TCAAACCCGG GGAGGCCAGC CGAGCCAATT TCACATTAAC
    CCCTCTGCCA CAAACCACAA GCTGGAGAGT CTCTCCCCAG GCACAGAGTA CACCATTTCA
    CTTGTTGCCC TCAAAGGCAA TCAGCAAAGT GCTAGCACTA CTGGTGTCTT TTCAACATTG
    GAACCTGTGG GATCAATTCC ACCTTACAAC ACTGAAGTCA CAGAGACTAC CATAGTGGTA
    ACTTGGACCC CTGTGCCCAG AATCGGGTTT AAGCTGGATG TTCGACCCAG CCAAGGTGGG
    GAGGCACCTC GGGAAGTCAT ATCCGAGTCT GGCAGCATTG TGATCTCTGG CCTAACCCCT
    GGAGTCGAAT ATATGTACAG CATATCTGTT CTTAAGGATG GCGTTGAGAG GGACATTCCC
    ATCACCAAGA CTGTTGTCAC ACCTGTCGCT CCTCCAACCA ACTTGCGCCT CCAACCAAGC
    AGAGATTCTG CTACACTTAC TGTTTACTGG GACAGTAGCA TTTCACCAGG AATCACTGGA
    TACAGAATTA CCACTGCACC CACTCCAATG CAGGTTGGCA ATTCCCTGGA AGAGGAGGTT
    GGTCCAACTC AGACATACTG CCTCTTTGAG AACCTGAGCC CTGGTGTAGA GTACAATGTC
    AGCGTGTATG CAGTAAAAGG GGAAGAGGAG AGTTCACCTC TCTCCCAGAT CTTTCTTCAA
    GAGATACCCC AACTGACTGA CCTAAAGTAT GACGATGTAA CTGACACAAG CATTGACCTG
    AGGTGGACGC CCCTTAATTC CTCCACCATT ATCGGCTACC GAATCACGGT GGTTGCGGCG
    GGAGAATCCG TCCCTATCTA CGAGGAATTT GTGGGCCCCA CAGATGGGTA TTACAAAGTT
    TCAGGATTGG AACCCGGCAT TGACTATGAG ATCAGCGTGA TAACACTTAT TAGCGGCGGA
    GAGAGCGCCC CGACCACAAT CGTACAGCAC ACGGCTGTTC CACCTCCAAC TAATTTGCGC
    TTCACCAATG TTGGCCCTGA CACCATGCGC GTCACCTGGT CACCTCCAAC TTCTATCGAA
    CTATCAAGTT TCCTGGTACG ATATTCTCCC GTGAAGAAGC CAGATGATGT GACAGAACTC
    TCCCTCTCTC CATCTACCAA CATGGTGGTT TTATCAAACT TGCTTCCCTT CACCGAATAT
    TTAGTCAGCG TTCATTCTGT TTATGAGGAG CGAGAGAGTA GTGCCCTCAA TGGTGTAGCT
    AAGACTTACT TGGACTCTCC AACTGGCATT GACTTTTCTG AAATTACTCC CAATTCATTT
    ACTGTGCACT GGATTGCTCC ACGTGCCCCT ATCACTGGCT ACAGAATTCG GTACCAGTTA
    GAAAGTGGAG CTGGGCGACC CAAGGAGGAG CGGGTACCTC CATCCCGGAA CTCTATTACC
    CTTACCCACC TCATTCCAGG TTCAGAATAC ATTGTCAGTA TCATTGCATT CAATGGTCAA
    CAGGAGAGTT TGCCACTTTC TGGACAGCAA GCCACTGTAT CTGATGTCCC AACTGATTTG
    GAAGTGACCT CCTCCTCTCC TAACACACTC ACCATCTCTT GGGAAGCACC TTCAGTGAAT
    GTACGTTACT ACAGGATCAC CCATTCCCAG ACAGGAGGTC ATGGTCCAGA GAAGGAGTTT
    ACAGTTCCAG GAACATCCAA CACTGCAACC ATCAGAGGGT TAAATCCAGG AGTAAGCTAC
    ACCATCACTG TTTATGCAGT GACTGGGCGA GGGGATAGTC CTGCCTCTAG CAAACCACTT
    ACCATTGTTC ACATGACAGA TGTTGACCAA CCAACTGACA TGGCTGTCAC AGATATCCAG
    GATCACAGCA TTCATGTCAA ATGGTCTCCT CCTCCAGGCC CAGTCACTGG TTACAGGGTC
    ACTAGTGTCC CAAAAAGTGG CCAAGGAGAA ACCTTTTCCC AGGTCGTTTC TCCAGATCAA
    ACTGAAGTGA CGATTGTGGG CCTGCAACCA GCTGTGGAGT ATGTGGTGAG CATATACTCA
    CAAGGTGAAA ATGGCGAGAG CGAGCCTCTG GTTGAAACTG CAGTTACAAA CATTGACCAT
    CCCAAAGGAC TGACATTCAC TGATGTGGGT GTTGATTCCA TTAGACTGGC TTGGGAGGTC
    CCAGATGGGC AGGTCACCCG CTACAGGGTT ACCTACTCAA GTCCTGAGGA GGGAGTAAAA
    GAGTTGTTCC CAGCTCCAGA AGGGGATGAT GACACAGCAG AATTGCACGG TCTGAGGCCG
    GGCACAGAAT ACACTGTGAG CATCGTGGCA TTGCACGATG ACATGGAGAG CAAGCCGATG
    ATTGGAATCC AGAGCACAGC AATTCCAGCA CCAACAAATC TTCAGTTCTC TCAAGTGACA
    CCAAGTGGCT TCTCTCTTAG CTGGCATGCA CCCACTATCC ATCTCAGTGG GTACCTTGTA
    CGTGTCAACC CTAAAGAGAA AACTGGGCCT ATGAAGGAAG TTAGACTTTC ACCTGGTGTG
    ACTGCTACAA CTGTAACTGG TCTGATGGTG GCCACAAAAT ATGAAGTTAA TGTGTATGCT
    CTTAAGGATA CCCTGACAAG TCAGCCACTG CAGGGTTTAA TCTCTACACT TGATAATGTA
    AGTCCACCTC GAAGACCTCG TGTTCAAGAT GTGACCGAGA CCACAGTTAC ACTGTCCTGG
    CGCACTAAAA CAGAGACCAT CACTGGCTTC CAGATTGATG CTATACCTGC CGGAGGTCAG
    AATCCCATCA GGAGAACAGT TGATGCAGAT CTCCGTTCTT TTACTATCAC AGGTCTGCAG
    CCTGGCACAG ACTACAAGAT CTATCTGTAC ACCCTAAATG ACAATGCTCG TAGCTCTCCT
    GTGATTGTTG ATGTCACCAC AGCTGTGGAC TCTCCATCCA ACCTGCGTTT CCTGACCACA
    ACTAGCAACT CCCTGCTGTT CACTTGGCAG CCACCTCGTG CTCGCATCAC TGGCTATATC
    ATTCGTTATG AGAAAGCTGG AGGGCTACTG AAAGAGCACC TCCCACGCCT CCAAGCAGGA
    ACCACAGAGT CTACCATTAC CAACCTGGAG CCAGGCACAG AATATATTGT CTATATTATT
    GCTGTGAGAA ACAATCAGAA GAGTGAGCCT CTTGTGGGAC GTAAGAGAAC AGGACCACAG
    CTAAATGATT CTGGCGTCCA AGAAGTGGCA TCCCATACTA CCATTTCATG GAGACCTGAG
    CTGGAAACCA CTGAATATAT CATCTCTTGC CATCCTATTG AACATGATGA GGCCCCATTG
    CAGTTTAGGG TCCCTGGAAC CTCCTCTAGT GCCACATTAA ATGGTCTGAC TCAAGGAGCC
    ACTTATAACA TCATTGTGGA AGCGCAGAAG GGGTCTGATA AACATAAGGT CTTCGAGAAG
    CGTGTAACTG TTGGCAGTCC TGGTTCCCCT GAGGGGGTTT TACAGCCAGC TGAGGACACC
    TGTTATGACA CATTCAATGG AGCACACTAT TCTGTGGGAC AGGAATGGGA ACGCATGTCA
    GAATCTGGAT TCAGGCTCTG GTGCAAGTGC CTGGGCTATG GAAGTGGACA CTTCAGATGT
    GACTCCTCCA AATGGTGCCA TGATAATGGT GTCAATCACA GGGTCGGGGA GAAATGGGAC
    CGACGAGGAG AGAATGGTCA GATGATGAGT TGTACTTGTC TTGGCAATGG AAAAGGAGAA
    TTTAAGTGTG AACCTCATGA AGCCACGTGC TATGATGAAG GGAAGATGTA CAATGTTGGA
    GAGCAGTGGC AGAAGGAATA TTTAGGGGCA ATCTGTTCAT GCACATGCTA TGGAGGACAG
    CAAGGGTGGA GATGTGACAA TTGCCGCAGA CCAGGTGCTG TTCTTCCCGA TGGCACTGCT
    GGGCATACAG TCTCTCAGTT TGCCCAGAGA TACCAACAGA ACTATAATTT GAACTGCCCC
    ATTGAGTGCT ACCTTCCCCT CCAAGCAGAT ATACAGCATT CGGAACAGAC CCAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825949.1
    CDS223..7302
    Translation

    Target ORF information:

    RefSeq Version XM_012970495.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis fibronectin 1 (fn1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970495.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCGCTGGG GGGCCCTGAC CGGGCTGCTC CTAGCCCTGT GCCTGTGTGC AGTGGTACGT 
    TCCGCCCCAT CAAGCAAGAA GCGCAGGCAG GCGCAGCAGC AGCAAGTAGT GCAACCCCAT
    GGATCACCGG ACAGCCAGCA GAAAGGATGT TATGACAATG GCAAGTACTA CCAAATCAAC
    CAGCAATGGG AGCGCACCTA CCTAGGGAAC ACACTGGTGT GTACTTGTTA TGGCGGAGGC
    AGAGGTTTCA ACTGCGAAAG CAAACCAGAG TCTGAAGAGA CCTGCTTTGA CAAATACACT
    GGTGTTACTT ACCGGGTCGG TGAAACATAT GAGCGACCAA AGGATAACAT GATCTGGGAC
    TGTACGTGTA TTGGAGCTGG AAGGGGACGC ATTAGCTGTA CAATTGCAAA TCGTTGCCAT
    GAAGGAGGTC AGTCTTATAA GATTGGGGAC ACCTGGCGCA GGCCACATGA AACTGGGGGG
    TATATGCTAG AATGTGTCTG CCTTGGAAAT GGTAAAGGAG AATGGACCTG CAAGCCCGTT
    GCTGAGAGAT GCTATGATAA CACAGCTGGG ATATCCTATG TAGTTGGTCA GACCTGGGAA
    AAGCCTTATC AGGGCTGGAT GATGGTGGAC TGTACATGCT TGGGAGAAGG CAGTGGACGA
    ATCACATGTT CTTCCAAAAA CAGATGCAAT GACCAGGACA CCAGAACTTC TTATAGAATT
    GGAAATTCAT GGAGTAAGAC TGACACCAGA GGAAATCTCT TGCAATGTAT CTGTACTGGC
    AATGGGCGAG GAGAGTGGAA ATGTGATAGA CATTCCTCTG CACAAGCAAC TGGTACTGGC
    TCAAACCCCA TCACAAACAT TCAAACTACC CTCTTCCAGC CTGATTCAGA GCTGGAGCCC
    TATGGTCACT GTGTGACTGA CAATGGGGTC TTGTATTCTT TGGGTATGAG ATGGCTGAAG
    GCACAGGGCA GCAAGCAGAT GCTCTGTACC TGCCTTGGTA ATGGTGTGAG CTGTGAAGAA
    ACTGTTGAAA CCATCACATT TGGTGGTAAT GCCAATGGTG AGCCATGTGC CATTCCTTTC
    ACTCATGATG GCAAGACCTA TTATTCCTGC ACAGGAGAGG GTCGTCAGGA TGGAAAATTG
    TGGTGTGCAA CAACCTCCAA CTATGACACT GACAAGAAGT ATTCATTCTG CAATGAGCAG
    CGTGCACTTG TACAGACACG TGGTGGTAAC TCCAATGGAG CTCTTTGCAA CTTTCCATTT
    TTGTACAACA ACCGCAACTA TACAGACTGC ACCTCTGAAG GTCGGAGGGA CAGTATGAAG
    TGGTGCGGGA CTACTGCCAA CTATGATGCC GATCAAAAAT TTGGATTCTG TCCCATGGCA
    GCTCATGAGG AAATATGCAC TACTAATGAG GGAGTCATGT ATAGAGTTGG TGATCAGTGG
    GACAAGCAGC ATGATCAAGG CCACATGATG CGATGTACTT GTGTTGGAAA TGGTCGTGGG
    GAATGGACTT GTGTGGCTTA TTCTCAGCTT AAAGACCAGT GCATTGTGGA TGGGATAACT
    TATGATGTGA ACAGTTCTTT TACGAAACGC CATGAGGAAG GACACATGAT GAACTGTACC
    TGCTATGGGC AGGGTCGTGG CAGATGGAAG TGCGATGCTA TAGACCAATG CCAGGATACT
    GAAACCCGAC AGTTCTATCA GATTGGAGAT TCCTGGGAAA AGCATTTGCA GGGAGTACGG
    TACCAGTGTT ACTGCTATGG CAAAGGAATT GGTGAATGGC ATTGCCAGCC TCTATCAACA
    TCCCCAGCTG GGACTGGACC CGTTCAGGTT ATTATCACAG AGAGTTCCAA TTTCCCTAAT
    TCTCACCCTA TACAGTGGAA TGCCCCACAG CCATCACACA TTAAGAATTA CATCCTGCGC
    TGGAAGCCTA AACTGAAGAC TGGCCCATGG AAACAAGCCA CTATTCCAGG ACACCTGAAC
    TCTTATACCA TCTCTGGCTT GAAACCTGGA ATCCTGTATG AGGGGCAATT GATCAGCATT
    CTGCAGTATG GAAACAGGGA GGTCACAACC TTTGACTTTA CCACCACTTC TACCATTCAC
    CGCTCCAGTC AAACTGAGAC AGGAGAAACT ACCCCACTTC CACCATTGGT GGCCACATCT
    GAGTCTGTAA CAGAAATTAC TGCTAGCAGT TTTCTGGTAT CCTGGGTCTC TGCATCTGAC
    ACAGTGTCTG GCTTCCGTGT TGAATATGAG CTGAGTGAGG ATGGAGATGA GAAACGCTAC
    CTAGAACTTC CAAACACTGC CACTTCAGTC AGTATCCCAG ACCTCCTCCC AGGCCGTAGA
    TACAATGTGA ATGTGTATCA GATAACTGAA GAGGGTGAAA AGAGCCTCAT CCTATCAACT
    ACTCAGACAA CTGCACCTGA TGCACCCCCC GACCATAATG TGGAAAATGT TGATGACACC
    TCCATCCTGA TCAAATGGAC CAAACCTCAA GCTCCCGTCA CAGGATACAG GGTTGTATAC
    AGTCCATCTG TAGAAGGCAG CAGCACAGAG TTAAACCTCC CAAGTACTGC AACTTCAGTG
    AGCCTGACTG AGCTGCTCCC AGGAATTGAA TACAATATTA CAATCTATGC AGTGGAAGAC
    AGTTTAGAAA GTGTTCCCAT CTTTATTCAG CAAGGGACAA CTGGAACTCC ACAGACAGTT
    ATAGTACCAT CTCCCACTGA CTTACAATTA GTGGAAGTTA CCGATATAAA AATTACCATC
    ATGTGGAACC CCCCACAAAG TGAGGTTTCT GGATACCGAG TTGTTGTGAA ACCTGTAAGT
    CCCTCTGACC GTGATGTGCA GAATCTCCCC GTGAGCAGGA ACACTTTTGC CGAGGTGGTT
    AACTTGCACC CTGGCAGGAC CTATACCTTT GAGGTGTATG CAGTTAATCG TGGACAAGAG
    AGTGAACCCC TGGCTGGAGA CTTCACTACC AAACTCGATG CTCCATCAGA CTTGCAGTTT
    ACAGATGTAA CTGACTCTAC AGTTGTTATT ATCTGGAGAC CACCCCAAGC CAAAATTGGT
    CGTTACCTGC TGTCTGTAGG TCAAACCCGG GGAGGCCAGC CGAGCCAATT TCACATTAAC
    CCCTCTGCCA CAAACCACAA GCTGGAGAGT CTCTCCCCAG GCACAGAGTA CACCATTTCA
    CTTGTTGCCC TCAAAGGCAA TCAGCAAAGT GCTAGCACTA CTGGTGTCTT TTCAACATTG
    GAACCTGTGG GATCAATTCC ACCTTACAAC ACTGAAGTCA CAGAGACTAC CATAGTGGTA
    ACTTGGACCC CTGTGCCCAG AATCGGGTTT AAGCTGGATG TTCGACCCAG CCAAGGTGGG
    GAGGCACCTC GGGAAGTCAT ATCCGAGTCT GGCAGCATTG TGATCTCTGG CCTAACCCCT
    GGAGTCGAAT ATATGTACAG CATATCTGTT CTTAAGGATG GCGTTGAGAG GGACATTCCC
    ATCACCAAGA CTGTTGTCAC ACCTGTCGCT CCTCCAACCA ACTTGCGCCT CCAACCAAGC
    AGAGATTCTG CTACACTTAC TGTTTACTGG GACAGTAGCA TTTCACCAGG AATCACTGGA
    TACAGAATTA CCACTGCACC CACTCCAATG CAGGTTGGCA ATTCCCTGGA AGAGGAGGTT
    GGTCCAACTC AGACATACTG CCTCTTTGAG AACCTGAGCC CTGGTGTAGA GTACAATGTC
    AGCGTGTATG CAGTAAAAGG GGAAGAGGAG AGTTCACCTC TCTCCCAGAT CTTTCTTCAA
    GAGATACCCC AACTGACTGA CCTAAAGTAT GACGATGTAA CTGACACAAG CATTGACCTG
    AGGTGGACGC CCCTTAATTC CTCCACCATT ATCGGCTACC GAATCACGGT GGTTGCGGCG
    GGAGAATCCG TCCCTATCTA CGAGGAATTT GTGGGCCCCA CAGATGGGTA TTACAAAGTT
    TCAGGATTGG AACCCGGCAT TGACTATGAG ATCAGCGTGA TAACACTTAT TAGCGGCGGA
    GAGAGCGCCC CGACCACAAT CGTACAGCAC ACGGCTGTTC CACCTCCAAC TAATTTGCGC
    TTCACCAATG TTGGCCCTGA CACCATGCGC GTCACCTGGT CACCTCCAAC TTCTATCGAA
    CTATCAAGTT TCCTGGTACG ATATTCTCCC GTGAAGAAGC CAGATGATGT GACAGAACTC
    TCCCTCTCTC CATCTACCAA CATGGTGGTT TTATCAAACT TGCTTCCCTT CACCGAATAT
    TTAGTCAGCG TTCATTCTGT TTATGAGGAG CGAGAGAGTA GTGCCCTCAA TGGTGTAGCT
    AAGACTTACT TGGACTCTCC AACTGGCATT GACTTTTCTG AAATTACTCC CAATTCATTT
    ACTGTGCACT GGATTGCTCC ACGTGCCCCT ATCACTGGCT ACAGAATTCG GTACCAGTTA
    GAAAGTGGAG CTGGGCGACC CAAGGAGGAG CGGGTACCTC CATCCCGGAA CTCTATTACC
    CTTACCCACC TCATTCCAGG TTCAGAATAC ATTGTCAGTA TCATTGCATT CAATGGTCAA
    CAGGAGAGTT TGCCACTTTC TGGACAGCAA GCCACTGTAT CTGATGTCCC AACTGATTTG
    GAAGTGACCT CCTCCTCTCC TAACACACTC ACCATCTCTT GGGAAGCACC TTCAGTGAAT
    GTACGTTACT ACAGGATCAC CCATTCCCAG ACAGGAGGTC ATGGTCCAGA GAAGGAGTTT
    ACAGTTCCAG GAACATCCAA CACTGCAACC ATCAGAGGGT TAAATCCAGG AGTAAGCTAC
    ACCATCACTG TTTATGCAGT GACTGGGCGA GGGGATAGTC CTGCCTCTAG CAAACCACTT
    ACCATTGTTC ACATGACAGA TGTTGACCAA CCAACTGACA TGGCTGTCAC AGATATCCAG
    GATCACAGCA TTCATGTCAA ATGGTCTCCT CCTCCAGGCC CAGTCACTGG TTACAGGGTC
    ACTAGTGTCC CAAAAAGTGG CCAAGGAGAA ACCTTTTCCC AGGTCGTTTC TCCAGATCAA
    ACTGAAGTGA CGATTGTGGG CCTGCAACCA GCTGTGGAGT ATGTGGTGAG CATATACTCA
    CAAGGTGAAA ATGGCGAGAG CGAGCCTCTG GTTGAAACTG CAGTTACAAA CATTGACCAT
    CCCAAAGGAC TGACATTCAC TGATGTGGGT GTTGATTCCA TTAGACTGGC TTGGGAGGTC
    CCAGATGGGC AGGTCACCCG CTACAGGGTT ACCTACTCAA GTCCTGAGGA GGGAGTAAAA
    GAGTTGTTCC CAGCTCCAGA AGGGGATGAT GACACAGCAG AATTGCACGG TCTGAGGCCG
    GGCACAGAAT ACACTGTGAG CATCGTGGCA TTGCACGATG ACATGGAGAG CAAGCCGATG
    ATTGGAATCC AGAGCACAGC AATTCCAGCA CCAACAAATC TTCAGTTCTC TCAAGTGACA
    CCAAGTGGCT TCTCTCTTAG CTGGCATGCA CCCACTATCC ATCTCAGTGG GTACCTTGTA
    CGTGTCAACC CTAAAGAGAA AACTGGGCCT ATGAAGGAAG TTAGACTTTC ACCTGGTGTG
    ACTGCTACAA CTGTAACTGG TCTGATGGTG GCCACAAAAT ATGAAGTTAA TGTGTATGCT
    CTTAAGGATA CCCTGACAAG TCAGCCACTG CAGGGTTTAA TCTCTACACT TGATAATGTA
    AGTCCACCTC GAAGACCTCG TGTTCAAGAT GTGACCGAGA CCACAGTTAC ACTGTCCTGG
    CGCACTAAAA CAGAGACCAT CACTGGCTTC CAGATTGATG CTATACCTGC CGGAGGTCAG
    AATCCCATCA GGAGAACAGT TGATGCAGAT CTCCGTTCTT TTACTATCAC AGGTCTGCAG
    CCTGGCACAG ACTACAAGAT CTATCTGTAC ACCCTAAATG ACAATGCTCG TAGCTCTCCT
    GTGATTGTTG ATGTCACCAC AGCTGTGGAC TCTCCATCCA ACCTGCGTTT CCTGACCACA
    ACTAGCAACT CCCTGCTGTT CACTTGGCAG CCACCTCGTG CTCGCATCAC TGGCTATATC
    ATTCGTTATG AGAAAGCTGG AGGGCTACTG AAAGAGCACC TCCCACGCCT CCAAGCAGGA
    ACCACAGAGT CTACCATTAC CAACCTGGAG CCAGGCACAG AATATATTGT CTATATTATT
    GCTGTGAGAA ACAATCAGAA GAGTGAGCCT CTTGTGGGAC GTAAGAGAAC AGGACCACAG
    CTAAATGATT CTGGCGTCCA AGAAGTGGCA TCCCATACTA CCATTTCATG GAGACCTGAG
    CTGGAAACCA CTGAATATAT CATCTCTTGC CATCCTATTG AACATGATGA GGCCCCATTG
    CAGTTTAGGG TCCCTGGAAC CTCCTCTAGT GCCACATTAA ATGGTCTGAC TCAAGGAGCC
    ACTTATAACA TCATTGTGGA AGCGCAGAAG GGGTCTGATA AACATAAGGT CTTCGAGAAG
    CGTGTAACTG TTGGCAGTCC TGGTTCCCCT GAGGGGGTTT TACAGCCAGC TGAGGACACC
    TGTTATGACA CATTCAATGG AGCACACTAT TCTGTGGGAC AGGAATGGGA ACGCATGTCA
    GAATCTGGAT TCAGGCTCTG GTGCAAGTGC CTGGGCTATG GAAGTGGACA CTTCAGATGT
    GACTCCTCCA AATGGTGCCA TGATAATGGT GTCAATCACA GGGTCGGGGA GAAATGGGAC
    CGACGAGGAG AGAATGGTCA GATGATGAGT TGTACTTGTC TTGGCAATGG AAAAGGAGAA
    TTTAAGTGTG AACCTCATGA AGCCACGTGC TATGATGAAG GGAAGATGTA CAATGTTGGA
    GAGCAGTGGC AGAAGGAATA TTTAGGGGCA ATCTGTTCAT GCACATGCTA TGGAGGACAG
    CAAGGGTGGA GATGTGACAA TTGCCGCAGA CCAGGTGCTG TTCTTCCCGA TGGCACTGCT
    GGGCATACAG TCTCTCAGTT TGCCCAGAGA TACCAACAGA ACTATAATTT GAACTGCCCC
    ATTGAGTGCT ACCTTCCCCT CCAAGCAGAT ATACAGCATT CGGAACAGAC CCAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa28347
    Clone ID Related Accession (Same CDS sequence) XM_012970496.2 , XM_012970496.3
    Accession Version XM_012970496.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product fibronectin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970496.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGCGCTGGG GGGCCCTGAC CGGGCTGCTC CTAGCCCTGT GCCTGTGTGC AGTGGTACGT 
    TCCGCCCCAT CAAGCAAGAA GCGCAGGCAG GCGCAGCAGC AGCAAGTAGT GCAACCCCAT
    GGATCACCGG ACAGCCAGCA GAAAGGATGT TATGACAATG GCAAGTACTA CCAAATCAAC
    CAGCAATGGG AGCGCACCTA CCTAGGGAAC ACACTGGTGT GTACTTGTTA TGGCGGAGGC
    AGAGGTTTCA ACTGCGAAAG CAAACCAGAG TCTGAAGAGA CCTGCTTTGA CAAATACACT
    GGTGTTACTT ACCGGGTCGG TGAAACATAT GAGCGACCAA AGGATAACAT GATCTGGGAC
    TGTACGTGTA TTGGAGCTGG AAGGGGACGC ATTAGCTGTA CAATTGCAAA TCGTTGCCAT
    GAAGGAGGTC AGTCTTATAA GATTGGGGAC ACCTGGCGCA GGCCACATGA AACTGGGGGG
    TATATGCTAG AATGTGTCTG CCTTGGAAAT GGTAAAGGAG AATGGACCTG CAAGCCCGTT
    GCTGAGAGAT GCTATGATAA CACAGCTGGG ATATCCTATG TAGTTGGTCA GACCTGGGAA
    AAGCCTTATC AGGGCTGGAT GATGGTGGAC TGTACATGCT TGGGAGAAGG CAGTGGACGA
    ATCACATGTT CTTCCAAAAA CAGATGCAAT GACCAGGACA CCAGAACTTC TTATAGAATT
    GGAAATTCAT GGAGTAAGAC TGACACCAGA GGAAATCTCT TGCAATGTAT CTGTACTGGC
    AATGGGCGAG GAGAGTGGAA ATGTGATAGA CATTCCTCTG CACAAGCAAC TGGTACTGGC
    TCAAACCCCA TCACAAACAT TCAAACTACC CTCTTCCAGC CTGATTCAGA GCTGGAGCCC
    TATGGTCACT GTGTGACTGA CAATGGGGTC TTGTATTCTT TGGGTATGAG ATGGCTGAAG
    GCACAGGGCA GCAAGCAGAT GCTCTGTACC TGCCTTGGTA ATGGTGTGAG CTGTGAAGAA
    ACTGTTGAAA CCATCACATT TGGTGGTAAT GCCAATGGTG AGCCATGTGC CATTCCTTTC
    ACTCATGATG GCAAGACCTA TTATTCCTGC ACAGGAGAGG GTCGTCAGGA TGGAAAATTG
    TGGTGTGCAA CAACCTCCAA CTATGACACT GACAAGAAGT ATTCATTCTG CAATGAGCAG
    CGTGCACTTG TACAGACACG TGGTGGTAAC TCCAATGGAG CTCTTTGCAA CTTTCCATTT
    TTGTACAACA ACCGCAACTA TACAGACTGC ACCTCTGAAG GTCGGAGGGA CAGTATGAAG
    TGGTGCGGGA CTACTGCCAA CTATGATGCC GATCAAAAAT TTGGATTCTG TCCCATGGCA
    GCTCATGAGG AAATATGCAC TACTAATGAG GGAGTCATGT ATAGAGTTGG TGATCAGTGG
    GACAAGCAGC ATGATCAAGG CCACATGATG CGATGTACTT GTGTTGGAAA TGGTCGTGGG
    GAATGGACTT GTGTGGCTTA TTCTCAGCTT AAAGACCAGT GCATTGTGGA TGGGATAACT
    TATGATGTGA ACAGTTCTTT TACGAAACGC CATGAGGAAG GACACATGAT GAACTGTACC
    TGCTATGGGC AGGGTCGTGG CAGATGGAAG TGCGATGCTA TAGACCAATG CCAGGATACT
    GAAACCCGAC AGTTCTATCA GATTGGAGAT TCCTGGGAAA AGCATTTGCA GGGAGTACGG
    TACCAGTGTT ACTGCTATGG CAAAGGAATT GGTGAATGGC ATTGCCAGCC TCTATCAACA
    TCCCCAGCTG GGACTGGACC CGTTCAGGTT ATTATCACAG AGAGTTCCAA TTTCCCTAAT
    TCTCACCCTA TACAGTGGAA TGCCCCACAG CCATCACACA TTAAGAATTA CATCCTGCGC
    TGGAAGCCTA AACTGAAGAC TGGCCCATGG AAACAAGCCA CTATTCCAGG ACACCTGAAC
    TCTTATACCA TCTCTGGCTT GAAACCTGGA ATCCTGTATG AGGGGCAATT GATCAGCATT
    CTGCAGTATG GAAACAGGGA GGTCACAACC TTTGACTTTA CCACCACTTC TACCATTCAC
    CGCTCCAGTC AAACTGAGAC AGGAGAAACT ACCCCACTTC CACCATTGGT GGCCACATCT
    GAGTCTGTAA CAGAAATTAC TGCTAGCAGT TTTCTGGTAT CCTGGGTCTC TGCATCTGAC
    ACAGTGTCTG GCTTCCGTGT TGAATATGAG CTGAGTGAGG ATGGAGATGA GAAACGCTAC
    CTAGAACTTC CAAACACTGC CACTTCAGTC AGTATCCCAG ACCTCCTCCC AGGCCGTAGA
    TACAATGTGA ATGTGTATCA GATAACTGAA GAGGGTGAAA AGAGCCTCAT CCTATCAACT
    ACTCAGACAA CTGCACCTGA TGCACCCCCC GACCATAATG TGGAAAATGT TGATGACACC
    TCCATCCTGA TCAAATGGAC CAAACCTCAA GCTCCCGTCA CAGGATACAG GGTTGTATAC
    AGTCCATCTG TAGAAGGCAG CAGCACAGAG TTAAACCTCC CAAGTACTGC AACTTCAGTG
    AGCCTGACTG AGCTGCTCCC AGGAATTGAA TACAATATTA CAATCTATGC AGTGGAAGAC
    AGTTTAGAAA GTGTTCCCAT CTTTATTCAG CAAGGGACAA CTGGAACTCC ACAGACAGTT
    ATAGTACCAT CTCCCACTGA CTTACAATTA GTGGAAGTTA CCGATATAAA AATTACCATC
    ATGTGGAACC CCCCACAAAG TGAGGTTTCT GGATACCGAG TTGTTGTGAA ACCTGTAAGT
    CCCTCTGACC GTGATGTGCA GAATCTCCCC GTGAGCAGGA ACACTTTTGC CGAGGTGGTT
    AACTTGCACC CTGGCAGGAC CTATACCTTT GAGGTGTATG CAGTTAATCG TGGACAAGAG
    AGTGAACCCC TGGCTGGAGA CTTCACTACC AAACTCGATG CTCCATCAGA CTTGCAGTTT
    ACAGATGTAA CTGACTCTAC AGTTGTTATT ATCTGGAGAC CACCCCAAGC CAAAATTGGT
    CGTTACCTGC TGTCTGTAGG TCAAACCCGG GGAGGCCAGC CGAGCCAATT TCACATTAAC
    CCCTCTGCCA CAAACCACAA GCTGGAGAGT CTCTCCCCAG GCACAGAGTA CACCATTTCA
    CTTGTTGCCC TCAAAGGCAA TCAGCAAAGT GCTAGCACTA CTGGTGTCTT TTCAACATTG
    GAACCTGTGG GATCAATTCC ACCTTACAAC ACTGAAGTCA CAGAGACTAC CATAGTGGTA
    ACTTGGACCC CTGTGCCCAG AATCGGGTTT AAGCTGGATG TTCGACCCAG CCAAGGTGGG
    GAGGCACCTC GGGAAGTCAT ATCCGAGTCT GGCAGCATTG TGATCTCTGG CCTAACCCCT
    GGAGTCGAAT ATATGTACAG CATATCTGTT CTTAAGGATG GCGTTGAGAG GGACATTCCC
    ATCACCAAGA CTGTTGTCAC ACCTGTCGCT CCTCCAACCA ACTTGCGCCT CCAACCAAGC
    AGAGATTCTG CTACACTTAC TGTTTACTGG GACAGTAGCA TTTCACCAGG AATCACTGGA
    TACAGAATTA CCACTGCACC CACTCCAATG CAGGTTGGCA ATTCCCTGGA AGAGGAGGTT
    GGTCCAACTC AGACATACTG CCTCTTTGAG AACCTGAGCC CTGGTGTAGA GTACAATGTC
    AGCGTGTATG CAGTAAAAGG GGAAGAGGAG AGTTCACCTC TCTCCCAGAT CTTTCTTCAA
    GCTGTTCCAC CTCCAACTAA TTTGCGCTTC ACCAATGTTG GCCCTGACAC CATGCGCGTC
    ACCTGGTCAC CTCCAACTTC TATCGAACTA TCAAGTTTCC TGGTACGATA TTCTCCCGTG
    AAGAAGCCAG ATGATGTGAC AGAACTCTCC CTCTCTCCAT CTACCAACAT GGTGGTTTTA
    TCAAACTTGC TTCCCTTCAC CGAATATTTA GTCAGCGTTC ATTCTGTTTA TGAGGAGCGA
    GAGAGTAGTG CCCTCAATGG TGTAGCTAAG ACTTACTTGG ACTCTCCAAC TGGCATTGAC
    TTTTCTGAAA TTACTCCCAA TTCATTTACT GTGCACTGGA TTGCTCCACG TGCCCCTATC
    ACTGGCTACA GAATTCGGTA CCAGTTAGAA AGTGGAGCTG GGCGACCCAA GGAGGAGCGG
    GTACCTCCAT CCCGGAACTC TATTACCCTT ACCCACCTCA TTCCAGGTTC AGAATACATT
    GTCAGTATCA TTGCATTCAA TGGTCAACAG GAGAGTTTGC CACTTTCTGG ACAGCAAGCC
    ACTGTATCTG ATGTCCCAAC TGATTTGGAA GTGACCTCCT CCTCTCCTAA CACACTCACC
    ATCTCTTGGG AAGCACCTTC AGTGAATGTA CGTTACTACA GGATCACCCA TTCCCAGACA
    GGAGGTCATG GTCCAGAGAA GGAGTTTACA GTTCCAGGAA CATCCAACAC TGCAACCATC
    AGAGGGTTAA ATCCAGGAGT AAGCTACACC ATCACTGTTT ATGCAGTGAC TGGGCGAGGG
    GATAGTCCTG CCTCTAGCAA ACCACTTACC ATTGTTCACA TGACAGATGT TGACCAACCA
    ACTGACATGG CTGTCACAGA TATCCAGGAT CACAGCATTC ATGTCAAATG GTCTCCTCCT
    CCAGGCCCAG TCACTGGTTA CAGGGTCACT AGTGTCCCAA AAAGTGGCCA AGGAGAAACC
    TTTTCCCAGG TCGTTTCTCC AGATCAAACT GAAGTGACGA TTGTGGGCCT GCAACCAGCT
    GTGGAGTATG TGGTGAGCAT ATACTCACAA GGTGAAAATG GCGAGAGCGA GCCTCTGGTT
    GAAACTGCAG TTACAACAAT TCCAGCACCA ACAAATCTTC AGTTCTCTCA AGTGACACCA
    AGTGGCTTCT CTCTTAGCTG GCATGCACCC ACTATCCATC TCAGTGGGTA CCTTGTACGT
    GTCAACCCTA AAGAGAAAAC TGGGCCTATG AAGGAAGTTA GACTTTCACC TGGTGTGACT
    GCTACAACTG TAACTGGTCT GATGGTGGCC ACAAAATATG AAGTTAATGT GTATGCTCTT
    AAGGATACCC TGACAAGTCA GCCACTGCAG GGTTTAATCT CTACACTTGA TAATGTAAGT
    CCACCTCGAA GACCTCGTGT TCAAGATGTG ACCGAGACCA CAGTTACACT GTCCTGGCGC
    ACTAAAACAG AGACCATCAC TGGCTTCCAG ATTGATGCTA TACCTGCCGG AGGTCAGAAT
    CCCATCAGGA GAACAGTTGA TGCAGATCTC CGTTCTTTTA CTATCACAGG TCTGCAGCCT
    GGCACAGACT ACAAGATCTA TCTGTACACC CTAAATGACA ATGCTCGTAG CTCTCCTGTG
    ATTGTTGATG TCACCACAGC TGTGGACTCT CCATCCAACC TGCGTTTCCT GACCACAACT
    AGCAACTCCC TGCTGTTCAC TTGGCAGCCA CCTCGTGCTC GCATCACTGG CTATATCATT
    CGTTATGAGA AAGCTGGAGG GCTACTGAAA GAGCACCTCC CACGCCTCCA AGCAGGAACC
    ACAGAGTCTA CCATTACCAA CCTGGAGCCA GGCACAGAAT ATATTGTCTA TATTATTGCT
    GTGAGAAACA ATCAGAAGAG TGAGCCTCTT GTGGGACGTA AGAGAACAGA CGAACTGCCT
    CGCCTGGTTA CACTTCCACA CCCGGGCAAA GGCCCTGAAA TCCTTGATGT TCCCACTGAT
    GAAGAGAACA CACCCCACAT CACACAAATC AAGTTGGACA ATGGTAATGG TATCCAACTG
    CCAGGCTCTA ATGGACAGCA GCCCAGTTCT GGCAATGAAG GGCAGCTTAT AGAAGAACAT
    GGTTTCAGAA GTCCCCTTGC ACCCACTACT GCTGTGCCAG TGAGGCCAGG GAAATTCACC
    CCTGGGCACT ACCCACAGGA GAGGGTTGAT ATTGAGCTAG ACACAGAGTT CCCTGTGCAG
    CGTGGAGATC TTGATGGTCC TTACCCTCAT GGCTTAGGAC CACAGCTAAA TGATTCTGGC
    GTCCAAGAAG TGGCATCCCA TACTACCATT TCATGGAGAC CTGAGCTGGA AACCACTGAA
    TATATCATCT CTTGCCATCC TATTGAACAT GATGAGGCCC CATTGCAGTT TAGGGTCCCT
    GGAACCTCCT CTAGTGCCAC ATTAAATGGT CTGACTCAAG GAGCCACTTA TAACATCATT
    GTGGAAGCGC AGAAGGGGTC TGATAAACAT AAGGTCTTCG AGAAGCGTGT AACTGTTGGC
    AGTCCTGGTT CCCCTGAGGG GGTTTTACAG CCAGCTGAGG ACACCTGTTA TGACACATTC
    AATGGAGCAC ACTATTCTGT GGGACAGGAA TGGGAACGCA TGTCAGAATC TGGATTCAGG
    CTCTGGTGCA AGTGCCTGGG CTATGGAAGT GGACACTTCA GATGTGACTC CTCCAAATGG
    TGCCATGATA ATGGTGTCAA TCACAGGGTC GGGGAGAAAT GGGACCGACG AGGAGAGAAT
    GGTCAGATGA TGAGTTGTAC TTGTCTTGGC AATGGAAAAG GAGAATTTAA GTGTGAACCT
    CATGAAGCCA CGTGCTATGA TGAAGGGAAG ATGTACAATG TTGGAGAGCA GTGGCAGAAG
    GAATATTTAG GGGCAATCTG TTCATGCACA TGCTATGGAG GACAGCAAGG GTGGAGATGT
    GACAATTGCC GCAGACCAGG TGCTGTTCTT CCCGATGGCA CTGCTGGGCA TACAGTCTCT
    CAGTTTGCCC AGAGATACCA ACAGAACTAT AATTTGAACT GCCCCATTGA GTGCTACCTT
    CCCCTCCAAG CAGATATACA GCATTCGGAA CAGACCCAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825950.1
    CDS222..7106
    Translation

    Target ORF information:

    RefSeq Version XM_012970496.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis fibronectin 1 (fn1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970496.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGCGCTGGG GGGCCCTGAC CGGGCTGCTC CTAGCCCTGT GCCTGTGTGC AGTGGTACGT 
    TCCGCCCCAT CAAGCAAGAA GCGCAGGCAG GCGCAGCAGC AGCAAGTAGT GCAACCCCAT
    GGATCACCGG ACAGCCAGCA GAAAGGATGT TATGACAATG GCAAGTACTA CCAAATCAAC
    CAGCAATGGG AGCGCACCTA CCTAGGGAAC ACACTGGTGT GTACTTGTTA TGGCGGAGGC
    AGAGGTTTCA ACTGCGAAAG CAAACCAGAG TCTGAAGAGA CCTGCTTTGA CAAATACACT
    GGTGTTACTT ACCGGGTCGG TGAAACATAT GAGCGACCAA AGGATAACAT GATCTGGGAC
    TGTACGTGTA TTGGAGCTGG AAGGGGACGC ATTAGCTGTA CAATTGCAAA TCGTTGCCAT
    GAAGGAGGTC AGTCTTATAA GATTGGGGAC ACCTGGCGCA GGCCACATGA AACTGGGGGG
    TATATGCTAG AATGTGTCTG CCTTGGAAAT GGTAAAGGAG AATGGACCTG CAAGCCCGTT
    GCTGAGAGAT GCTATGATAA CACAGCTGGG ATATCCTATG TAGTTGGTCA GACCTGGGAA
    AAGCCTTATC AGGGCTGGAT GATGGTGGAC TGTACATGCT TGGGAGAAGG CAGTGGACGA
    ATCACATGTT CTTCCAAAAA CAGATGCAAT GACCAGGACA CCAGAACTTC TTATAGAATT
    GGAAATTCAT GGAGTAAGAC TGACACCAGA GGAAATCTCT TGCAATGTAT CTGTACTGGC
    AATGGGCGAG GAGAGTGGAA ATGTGATAGA CATTCCTCTG CACAAGCAAC TGGTACTGGC
    TCAAACCCCA TCACAAACAT TCAAACTACC CTCTTCCAGC CTGATTCAGA GCTGGAGCCC
    TATGGTCACT GTGTGACTGA CAATGGGGTC TTGTATTCTT TGGGTATGAG ATGGCTGAAG
    GCACAGGGCA GCAAGCAGAT GCTCTGTACC TGCCTTGGTA ATGGTGTGAG CTGTGAAGAA
    ACTGTTGAAA CCATCACATT TGGTGGTAAT GCCAATGGTG AGCCATGTGC CATTCCTTTC
    ACTCATGATG GCAAGACCTA TTATTCCTGC ACAGGAGAGG GTCGTCAGGA TGGAAAATTG
    TGGTGTGCAA CAACCTCCAA CTATGACACT GACAAGAAGT ATTCATTCTG CAATGAGCAG
    CGTGCACTTG TACAGACACG TGGTGGTAAC TCCAATGGAG CTCTTTGCAA CTTTCCATTT
    TTGTACAACA ACCGCAACTA TACAGACTGC ACCTCTGAAG GTCGGAGGGA CAGTATGAAG
    TGGTGCGGGA CTACTGCCAA CTATGATGCC GATCAAAAAT TTGGATTCTG TCCCATGGCA
    GCTCATGAGG AAATATGCAC TACTAATGAG GGAGTCATGT ATAGAGTTGG TGATCAGTGG
    GACAAGCAGC ATGATCAAGG CCACATGATG CGATGTACTT GTGTTGGAAA TGGTCGTGGG
    GAATGGACTT GTGTGGCTTA TTCTCAGCTT AAAGACCAGT GCATTGTGGA TGGGATAACT
    TATGATGTGA ACAGTTCTTT TACGAAACGC CATGAGGAAG GACACATGAT GAACTGTACC
    TGCTATGGGC AGGGTCGTGG CAGATGGAAG TGCGATGCTA TAGACCAATG CCAGGATACT
    GAAACCCGAC AGTTCTATCA GATTGGAGAT TCCTGGGAAA AGCATTTGCA GGGAGTACGG
    TACCAGTGTT ACTGCTATGG CAAAGGAATT GGTGAATGGC ATTGCCAGCC TCTATCAACA
    TCCCCAGCTG GGACTGGACC CGTTCAGGTT ATTATCACAG AGAGTTCCAA TTTCCCTAAT
    TCTCACCCTA TACAGTGGAA TGCCCCACAG CCATCACACA TTAAGAATTA CATCCTGCGC
    TGGAAGCCTA AACTGAAGAC TGGCCCATGG AAACAAGCCA CTATTCCAGG ACACCTGAAC
    TCTTATACCA TCTCTGGCTT GAAACCTGGA ATCCTGTATG AGGGGCAATT GATCAGCATT
    CTGCAGTATG GAAACAGGGA GGTCACAACC TTTGACTTTA CCACCACTTC TACCATTCAC
    CGCTCCAGTC AAACTGAGAC AGGAGAAACT ACCCCACTTC CACCATTGGT GGCCACATCT
    GAGTCTGTAA CAGAAATTAC TGCTAGCAGT TTTCTGGTAT CCTGGGTCTC TGCATCTGAC
    ACAGTGTCTG GCTTCCGTGT TGAATATGAG CTGAGTGAGG ATGGAGATGA GAAACGCTAC
    CTAGAACTTC CAAACACTGC CACTTCAGTC AGTATCCCAG ACCTCCTCCC AGGCCGTAGA
    TACAATGTGA ATGTGTATCA GATAACTGAA GAGGGTGAAA AGAGCCTCAT CCTATCAACT
    ACTCAGACAA CTGCACCTGA TGCACCCCCC GACCATAATG TGGAAAATGT TGATGACACC
    TCCATCCTGA TCAAATGGAC CAAACCTCAA GCTCCCGTCA CAGGATACAG GGTTGTATAC
    AGTCCATCTG TAGAAGGCAG CAGCACAGAG TTAAACCTCC CAAGTACTGC AACTTCAGTG
    AGCCTGACTG AGCTGCTCCC AGGAATTGAA TACAATATTA CAATCTATGC AGTGGAAGAC
    AGTTTAGAAA GTGTTCCCAT CTTTATTCAG CAAGGGACAA CTGGAACTCC ACAGACAGTT
    ATAGTACCAT CTCCCACTGA CTTACAATTA GTGGAAGTTA CCGATATAAA AATTACCATC
    ATGTGGAACC CCCCACAAAG TGAGGTTTCT GGATACCGAG TTGTTGTGAA ACCTGTAAGT
    CCCTCTGACC GTGATGTGCA GAATCTCCCC GTGAGCAGGA ACACTTTTGC CGAGGTGGTT
    AACTTGCACC CTGGCAGGAC CTATACCTTT GAGGTGTATG CAGTTAATCG TGGACAAGAG
    AGTGAACCCC TGGCTGGAGA CTTCACTACC AAACTCGATG CTCCATCAGA CTTGCAGTTT
    ACAGATGTAA CTGACTCTAC AGTTGTTATT ATCTGGAGAC CACCCCAAGC CAAAATTGGT
    CGTTACCTGC TGTCTGTAGG TCAAACCCGG GGAGGCCAGC CGAGCCAATT TCACATTAAC
    CCCTCTGCCA CAAACCACAA GCTGGAGAGT CTCTCCCCAG GCACAGAGTA CACCATTTCA
    CTTGTTGCCC TCAAAGGCAA TCAGCAAAGT GCTAGCACTA CTGGTGTCTT TTCAACATTG
    GAACCTGTGG GATCAATTCC ACCTTACAAC ACTGAAGTCA CAGAGACTAC CATAGTGGTA
    ACTTGGACCC CTGTGCCCAG AATCGGGTTT AAGCTGGATG TTCGACCCAG CCAAGGTGGG
    GAGGCACCTC GGGAAGTCAT ATCCGAGTCT GGCAGCATTG TGATCTCTGG CCTAACCCCT
    GGAGTCGAAT ATATGTACAG CATATCTGTT CTTAAGGATG GCGTTGAGAG GGACATTCCC
    ATCACCAAGA CTGTTGTCAC ACCTGTCGCT CCTCCAACCA ACTTGCGCCT CCAACCAAGC
    AGAGATTCTG CTACACTTAC TGTTTACTGG GACAGTAGCA TTTCACCAGG AATCACTGGA
    TACAGAATTA CCACTGCACC CACTCCAATG CAGGTTGGCA ATTCCCTGGA AGAGGAGGTT
    GGTCCAACTC AGACATACTG CCTCTTTGAG AACCTGAGCC CTGGTGTAGA GTACAATGTC
    AGCGTGTATG CAGTAAAAGG GGAAGAGGAG AGTTCACCTC TCTCCCAGAT CTTTCTTCAA
    GCTGTTCCAC CTCCAACTAA TTTGCGCTTC ACCAATGTTG GCCCTGACAC CATGCGCGTC
    ACCTGGTCAC CTCCAACTTC TATCGAACTA TCAAGTTTCC TGGTACGATA TTCTCCCGTG
    AAGAAGCCAG ATGATGTGAC AGAACTCTCC CTCTCTCCAT CTACCAACAT GGTGGTTTTA
    TCAAACTTGC TTCCCTTCAC CGAATATTTA GTCAGCGTTC ATTCTGTTTA TGAGGAGCGA
    GAGAGTAGTG CCCTCAATGG TGTAGCTAAG ACTTACTTGG ACTCTCCAAC TGGCATTGAC
    TTTTCTGAAA TTACTCCCAA TTCATTTACT GTGCACTGGA TTGCTCCACG TGCCCCTATC
    ACTGGCTACA GAATTCGGTA CCAGTTAGAA AGTGGAGCTG GGCGACCCAA GGAGGAGCGG
    GTACCTCCAT CCCGGAACTC TATTACCCTT ACCCACCTCA TTCCAGGTTC AGAATACATT
    GTCAGTATCA TTGCATTCAA TGGTCAACAG GAGAGTTTGC CACTTTCTGG ACAGCAAGCC
    ACTGTATCTG ATGTCCCAAC TGATTTGGAA GTGACCTCCT CCTCTCCTAA CACACTCACC
    ATCTCTTGGG AAGCACCTTC AGTGAATGTA CGTTACTACA GGATCACCCA TTCCCAGACA
    GGAGGTCATG GTCCAGAGAA GGAGTTTACA GTTCCAGGAA CATCCAACAC TGCAACCATC
    AGAGGGTTAA ATCCAGGAGT AAGCTACACC ATCACTGTTT ATGCAGTGAC TGGGCGAGGG
    GATAGTCCTG CCTCTAGCAA ACCACTTACC ATTGTTCACA TGACAGATGT TGACCAACCA
    ACTGACATGG CTGTCACAGA TATCCAGGAT CACAGCATTC ATGTCAAATG GTCTCCTCCT
    CCAGGCCCAG TCACTGGTTA CAGGGTCACT AGTGTCCCAA AAAGTGGCCA AGGAGAAACC
    TTTTCCCAGG TCGTTTCTCC AGATCAAACT GAAGTGACGA TTGTGGGCCT GCAACCAGCT
    GTGGAGTATG TGGTGAGCAT ATACTCACAA GGTGAAAATG GCGAGAGCGA GCCTCTGGTT
    GAAACTGCAG TTACAACAAT TCCAGCACCA ACAAATCTTC AGTTCTCTCA AGTGACACCA
    AGTGGCTTCT CTCTTAGCTG GCATGCACCC ACTATCCATC TCAGTGGGTA CCTTGTACGT
    GTCAACCCTA AAGAGAAAAC TGGGCCTATG AAGGAAGTTA GACTTTCACC TGGTGTGACT
    GCTACAACTG TAACTGGTCT GATGGTGGCC ACAAAATATG AAGTTAATGT GTATGCTCTT
    AAGGATACCC TGACAAGTCA GCCACTGCAG GGTTTAATCT CTACACTTGA TAATGTAAGT
    CCACCTCGAA GACCTCGTGT TCAAGATGTG ACCGAGACCA CAGTTACACT GTCCTGGCGC
    ACTAAAACAG AGACCATCAC TGGCTTCCAG ATTGATGCTA TACCTGCCGG AGGTCAGAAT
    CCCATCAGGA GAACAGTTGA TGCAGATCTC CGTTCTTTTA CTATCACAGG TCTGCAGCCT
    GGCACAGACT ACAAGATCTA TCTGTACACC CTAAATGACA ATGCTCGTAG CTCTCCTGTG
    ATTGTTGATG TCACCACAGC TGTGGACTCT CCATCCAACC TGCGTTTCCT GACCACAACT
    AGCAACTCCC TGCTGTTCAC TTGGCAGCCA CCTCGTGCTC GCATCACTGG CTATATCATT
    CGTTATGAGA AAGCTGGAGG GCTACTGAAA GAGCACCTCC CACGCCTCCA AGCAGGAACC
    ACAGAGTCTA CCATTACCAA CCTGGAGCCA GGCACAGAAT ATATTGTCTA TATTATTGCT
    GTGAGAAACA ATCAGAAGAG TGAGCCTCTT GTGGGACGTA AGAGAACAGA CGAACTGCCT
    CGCCTGGTTA CACTTCCACA CCCGGGCAAA GGCCCTGAAA TCCTTGATGT TCCCACTGAT
    GAAGAGAACA CACCCCACAT CACACAAATC AAGTTGGACA ATGGTAATGG TATCCAACTG
    CCAGGCTCTA ATGGACAGCA GCCCAGTTCT GGCAATGAAG GGCAGCTTAT AGAAGAACAT
    GGTTTCAGAA GTCCCCTTGC ACCCACTACT GCTGTGCCAG TGAGGCCAGG GAAATTCACC
    CCTGGGCACT ACCCACAGGA GAGGGTTGAT ATTGAGCTAG ACACAGAGTT CCCTGTGCAG
    CGTGGAGATC TTGATGGTCC TTACCCTCAT GGCTTAGGAC CACAGCTAAA TGATTCTGGC
    GTCCAAGAAG TGGCATCCCA TACTACCATT TCATGGAGAC CTGAGCTGGA AACCACTGAA
    TATATCATCT CTTGCCATCC TATTGAACAT GATGAGGCCC CATTGCAGTT TAGGGTCCCT
    GGAACCTCCT CTAGTGCCAC ATTAAATGGT CTGACTCAAG GAGCCACTTA TAACATCATT
    GTGGAAGCGC AGAAGGGGTC TGATAAACAT AAGGTCTTCG AGAAGCGTGT AACTGTTGGC
    AGTCCTGGTT CCCCTGAGGG GGTTTTACAG CCAGCTGAGG ACACCTGTTA TGACACATTC
    AATGGAGCAC ACTATTCTGT GGGACAGGAA TGGGAACGCA TGTCAGAATC TGGATTCAGG
    CTCTGGTGCA AGTGCCTGGG CTATGGAAGT GGACACTTCA GATGTGACTC CTCCAAATGG
    TGCCATGATA ATGGTGTCAA TCACAGGGTC GGGGAGAAAT GGGACCGACG AGGAGAGAAT
    GGTCAGATGA TGAGTTGTAC TTGTCTTGGC AATGGAAAAG GAGAATTTAA GTGTGAACCT
    CATGAAGCCA CGTGCTATGA TGAAGGGAAG ATGTACAATG TTGGAGAGCA GTGGCAGAAG
    GAATATTTAG GGGCAATCTG TTCATGCACA TGCTATGGAG GACAGCAAGG GTGGAGATGT
    GACAATTGCC GCAGACCAGG TGCTGTTCTT CCCGATGGCA CTGCTGGGCA TACAGTCTCT
    CAGTTTGCCC AGAGATACCA ACAGAACTAT AATTTGAACT GCCCCATTGA GTGCTACCTT
    CCCCTCCAAG CAGATATACA GCATTCGGAA CAGACCCAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.