ORF » Species Summary » Xenopus tropicalis » pigt cDNA ORF clone

pigt cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following pigt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pigt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa21118 NM_001017115.2
Latest version!		 Xenopus tropicalis phosphatidylinositol glycan anchor biosynthesis class T (pigt), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OXa28072 XM_012971797.2
Latest version!		 Xenopus tropicalis phosphatidylinositol glycan anchor biosynthesis class T (pigt), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OXa50129 XM_012971797.3
Latest version!		 Xenopus tropicalis phosphatidylinositol glycan anchor biosynthesis class T (pigt), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    pigt ( NM_001017115.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001017115.1 Xenopus tropicalis phosphatidylinositol glycan anchor biosynthesis class T (pigt), mRNA.
    Price & Availability↑
    CloneID OXa21118
    Clone ID Related Accession (Same CDS sequence) NM_001017115.2
    Accession Version NM_001017115.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GPI transamidase component PIG-T precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR761188.2. On Oct 14, 2005 this sequence version replaced NM_001017115.1. ##Evidence-Data-START## Transcript exon combination :: CR761188.2, BC099698.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCCGGCG CTGTGCTTGT AGCCGCTGCT CTGTGGGCCA GCGTTTGGGC CGGGCAGTGT 
    GCCGAGCCGC CTGGGAGTGA TGTCTTCCGA GAGGAGCTGC TCCTCACACC GCTGCGCTCC 
    GGGGACATCG CCGCCACCTT TCAGTTTCGG ACCCGCCTGG ATGCCGACCT GAGGAAGGAG 
    AAGGTCTCCC ACTACAGGAT CTTCCCTAAA GTGCTGGGCC AGCTCATCTC TAAGTACTCC 
    ATTCAGGAGC TGCACCTTTC CTTCACCCAG GGATTCTGGC GTACAAAGGC ATGGGGCCCA 
    CCTCTCCTGC AGGCCCCCAC TGGAGCAGAA CTCTGGGTCT GGTTTCAGGA TTCTGTTACT 
    GATGTTAATA AAAGTTGGAA AGAGCTCAGT AACATCTTAT CGGGTATTTT CTGTGCCTCT 
    CTGAACTTCA TCGACTCTAC TAACACAGTT ACTCCGACTG CATCCTTCAA GCCCCTTGGA 
    GTGGCCAACA TTACAGATCA TCACCTGCTC AGATATGCCA CGCTTCCCAG AGAGATAGTT 
    TGCACAGAAA ATCTAACTCC ATGGAAGAAA CTGCTGCCTT GTGGGTCCAA GGCCGGCCTG 
    GCATCTCTAC TGAGGGCTGA ACGCTTGTAT CACAGCAGTT ACCACTCCCA GTCTGTGCAT 
    ATAAGGCCAA TTTGCCAGAA TGCAGATTGT TCAAGTGTCT CCTGGGAGTT AAGGCAGGCC 
    CTTACTGTGG TGTTCGATAT GTATTCTAGT GGCCAGGGAA AAAGAGATTG GTCCATATTT 
    AAAATGTTTT CCCGTACTGT AACTGAGTCG TGTCCTCTGG CATCACAAAG TAAAATCTAT 
    GTTGACGTTT CTGGCTCGGG AAAGGAAGTT GATCTCTTTG AGCTGAACCC TCCTACCTCC 
    TTGGAAAGAC AGGTGGTGGT TCACGGAGAC CGCAGAGTGT ATGCAGAATA CGACCTTTCC 
    CAGCAGGGCA CATTCTCCAC ATCCAGAAAC CTTAATGTAG CCCTTAAGTG GAAAGGGCGG 
    ACCGCTGAGT CAGCAGGCCA GCCACAGTCC CCCCTCTTTC ATGCCGAACG CTACGTGAGT 
    GGATATGGGT TGCAGTCTGG AGTGATCAAC ACTATGGTAT TTAACAATCA CCCGTATAGA 
    GCCTACCCTG TTATGCTGCT GGAGACAGTG CCTTGGTACC TTCGCCTGTA TGTGCACACA 
    CTGACAATCA CTACCAGGGG CAAGGAGAAC AAACCCAGCT ACATTCATTA TGAACCGGCA 
    AAGGATCGCC AGCGCCCACA CTTGTTAGAG ATGCTGATAC AGCTGCCTCC AAACTCCATT 
    ACTAAAATCA GCATCCAGTT TGAGAGGGCG CTACTGAAAT GGACGGAATA CCCCCCTGAT 
    CCTAACCATG GCTTCTACAT CAGTCCCTCT GTTCTGAGCG TGCTCATTCC CAGTGTTATT 
    AACCAGAAGA CGGACGAACT AGAGGAAAGC ACACTGTTCA GCACTCTGTT TCCATCATCA 
    GATGGTTCAA GCTACTTCAT GCGTCTGTAC ACAGAACCCC TACTGGTTAA CCTTCCCACA 
    CCAGACTTCA GCATGCCCTA CAATGTCATC TGCCTAACTT GCACTGTGGT GGCCGTGGGC 
    TACGGCTCTT TTTATAATCT GCTCACCAGG ACATTCCAGG TTGAAGATAG AAAAGGAGGT 
    GGCCTGGCTA AGACTATAGC CAATCTGATA CGCAGGTTGC GTGGGGTACC ACCTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001017115.1
    CDS13..1752
    Translation

    Target ORF information:

    RefSeq Version NM_001017115.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphatidylinositol glycan anchor biosynthesis class T (pigt), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001017115.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCCGGCG CTGTGCTTGT AGCCGCTGCT CTGTGGGCCA GCGTTTGGGC CGGGCAGTGT 
    GCCGAGCCGC CTGGGAGTGA TGTCTTCCGA GAGGAGCTGC TCCTCACACC GCTGCGCTCC 
    GGGGACATCG CCGCCACCTT TCAGTTTCGG ACCCGCCTGG ATGCCGACCT GAGGAAGGAG 
    AAGGTCTCCC ACTACAGGAT CTTCCCTAAA GTGCTGGGCC AGCTCATCTC TAAGTACTCC 
    ATTCAGGAGC TGCACCTTTC CTTCACCCAG GGATTCTGGC GTACAAAGGC ATGGGGCCCA 
    CCTCTCCTGC AGGCCCCCAC TGGAGCAGAA CTCTGGGTCT GGTTTCAGGA TTCTGTTACT 
    GATGTTAATA AAAGTTGGAA AGAGCTCAGT AACATCTTAT CGGGTATTTT CTGTGCCTCT 
    CTGAACTTCA TCGACTCTAC TAACACAGTT ACTCCGACTG CATCCTTCAA GCCCCTTGGA 
    GTGGCCAACA TTACAGATCA TCACCTGCTC AGATATGCCA CGCTTCCCAG AGAGATAGTT 
    TGCACAGAAA ATCTAACTCC ATGGAAGAAA CTGCTGCCTT GTGGGTCCAA GGCCGGCCTG 
    GCATCTCTAC TGAGGGCTGA ACGCTTGTAT CACAGCAGTT ACCACTCCCA GTCTGTGCAT 
    ATAAGGCCAA TTTGCCAGAA TGCAGATTGT TCAAGTGTCT CCTGGGAGTT AAGGCAGGCC 
    CTTACTGTGG TGTTCGATAT GTATTCTAGT GGCCAGGGAA AAAGAGATTG GTCCATATTT 
    AAAATGTTTT CCCGTACTGT AACTGAGTCG TGTCCTCTGG CATCACAAAG TAAAATCTAT 
    GTTGACGTTT CTGGCTCGGG AAAGGAAGTT GATCTCTTTG AGCTGAACCC TCCTACCTCC 
    TTGGAAAGAC AGGTGGTGGT TCACGGAGAC CGCAGAGTGT ATGCAGAATA CGACCTTTCC 
    CAGCAGGGCA CATTCTCCAC ATCCAGAAAC CTTAATGTAG CCCTTAAGTG GAAAGGGCGG 
    ACCGCTGAGT CAGCAGGCCA GCCACAGTCC CCCCTCTTTC ATGCCGAACG CTACGTGAGT 
    GGATATGGGT TGCAGTCTGG AGTGATCAAC ACTATGGTAT TTAACAATCA CCCGTATAGA 
    GCCTACCCTG TTATGCTGCT GGAGACAGTG CCTTGGTACC TTCGCCTGTA TGTGCACACA 
    CTGACAATCA CTACCAGGGG CAAGGAGAAC AAACCCAGCT ACATTCATTA TGAACCGGCA 
    AAGGATCGCC AGCGCCCACA CTTGTTAGAG ATGCTGATAC AGCTGCCTCC AAACTCCATT 
    ACTAAAATCA GCATCCAGTT TGAGAGGGCG CTACTGAAAT GGACGGAATA CCCCCCTGAT 
    CCTAACCATG GCTTCTACAT CAGTCCCTCT GTTCTGAGCG TGCTCATTCC CAGTGTTATT 
    AACCAGAAGA CGGACGAACT AGAGGAAAGC ACACTGTTCA GCACTCTGTT TCCATCATCA 
    GATGGTTCAA GCTACTTCAT GCGTCTGTAC ACAGAACCCC TACTGGTTAA CCTTCCCACA 
    CCAGACTTCA GCATGCCCTA CAATGTCATC TGCCTAACTT GCACTGTGGT GGCCGTGGGC 
    TACGGCTCTT TTTATAATCT GCTCACCAGG ACATTCCAGG TTGAAGATAG AAAAGGAGGT 
    GGCCTGGCTA AGACTATAGC CAATCTGATA CGCAGGTTGC GTGGGGTACC ACCTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    pigt ( XM_012971797.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827251.1 Xenopus tropicalis phosphatidylinositol glycan anchor biosynthesis class T (pigt), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OXa28072
    Clone ID Related Accession (Same CDS sequence) XM_012971797.2
    Accession Version XM_012971797.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1737bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GPI transamidase component PIG-T isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971797.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCCGGCG CTGTGCTTGT AGCCGCTGCT CTGTGGGCCA GCGTTTGGGC CGGGCAGTGT 
    GCCGAGCCGC CTGGGAGTGA TGTCTTCCGA GAGGAGCTGC TCCTCACACC GCTGCGCTCC 
    GGGGACATCG CCGCCACCTT TCAGTTTCGG ACCCGCCTGG ATGCCGACCT GAGGAAGGAG 
    AAGGTCTCCC ACTACAGGAT CTTCCCTAAA GTGCTGGGCC AGCTCATCTC TAAGTACTCC 
    ATTCAGGAGC TGCACCTTTC CTTCACCCAG GGATTCTGGC GTACAAAGGC ATGGGGCCCA 
    CCTCTCCTGC AGGCCCCCAC TGGAGCAGAA CTCTGGGTCT GGTTTCAGGA TTCTGTTACT 
    GATGTTAATA AAAGTTGGAA AGAGCTCAGT AACATCTTAT CGGGTATTTT CTGTGCCTCT 
    CTGAACTTCA TCGACTCTAC TAACACAGTT ACTCCGACTG CATCCTTCAA GCCCCTTGGA 
    GTGGCCAACA TTACAGATCA TCACCTGCTC AGATATGCCA CGCTTCCCAG AGAGATAGTT 
    TGCACAGAAA ATCTAACTCC ATGGAAGAAA CTGCTGCCTT GTGGGTCCAA GGCCGGCCTG 
    GCATCTCTAC TGAGGGCTGA ACGCTTGTAT CACAGCAGTT ACCACTCCCA GTCTGTGCAT 
    ATAAGGCCAA TTTGCCAGAA TGCAGATTGT TCAAGTGTCT CCTGGGAGTT AAGGCAGGCC 
    CTTACTGTGG TGTTCGATAT GTATTCTAGT GGCCAGGGAA AAAGAGATTG GTCCATATTT 
    AAAATGTTTT CCCGTACTGT AACTGAGTCG TGTCCTCTGG CATCACAAAG TAAAATCTAT 
    GTTGACGTTT CTGGCTCGGG AAAGGAAGTT GATCTCTTTG AGCTGAACCC TCCTACCTCC 
    TTGGAAAGAC AGGTGGTGGT TCACGGAGAC CGCAGAGTGT ATGCAGAATA CGACCTTTCC 
    CAGCAGGGCA CATTCTCCAC ATCCAGAAAC CTTAATGTAG CCCTTAAGTG GAAAGGGCGG 
    ACCGCTGAGT CAGGCCAGCC ACAGTCCCCC CTCTTTCATG CCGAACGCTA CGTGAGTGGA 
    TATGGGTTGC AGTCTGGAGT GATCAACACT ATGGTATTTA ACAATCACCC GTATAGAGCC 
    TACCCTGTTA TGCTGCTGGA GACAGTGCCT TGGTACCTTC GCCTGTATGT GCACACACTG 
    ACAATCACTA CCAGGGGCAA GGAGAACAAA CCCAGCTACA TTCATTATGA ACCGGCAAAG 
    GATCGCCAGC GCCCACACTT GTTAGAGATG CTGATACAGC TGCCTCCAAA CTCCATTACT 
    AAAATCAGCA TCCAGTTTGA GAGGGCGCTA CTGAAATGGA CGGAATACCC CCCTGATCCT 
    AACCATGGCT TCTACATCAG TCCCTCTGTT CTGAGCGTGC TCATTCCCAG TGTTATTAAC 
    CAGAAGACGG ACGAACTAGA GGAAAGCACA CTGTTCAGCA CTCTGTTTCC ATCATCAGAT 
    GGTTCAAGCT ACTTCATGCG TCTGTACACA GAACCCCTAC TGGTTAACCT TCCCACACCA 
    GACTTCAGCA TGCCCTACAA TGTCATCTGC CTAACTTGCA CTGTGGTGGC CGTGGGCTAC 
    GGCTCTTTTT ATAATCTGCT CACCAGGACA TTCCAGGTTG AAGATAGAAA AGGAGGTGGC 
    CTGGCTAAGA CTATAGCCAA TCTGATACGC AGGTTGCGTG GGGTACCACC TTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012827251.1
    CDS90..1826
    Translation

    Target ORF information:

    RefSeq Version XM_012971797.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphatidylinositol glycan anchor biosynthesis class T (pigt), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012971797.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCCGGCG CTGTGCTTGT AGCCGCTGCT CTGTGGGCCA GCGTTTGGGC CGGGCAGTGT 
    GCCGAGCCGC CTGGGAGTGA TGTCTTCCGA GAGGAGCTGC TCCTCACACC GCTGCGCTCC 
    GGGGACATCG CCGCCACCTT TCAGTTTCGG ACCCGCCTGG ATGCCGACCT GAGGAAGGAG 
    AAGGTCTCCC ACTACAGGAT CTTCCCTAAA GTGCTGGGCC AGCTCATCTC TAAGTACTCC 
    ATTCAGGAGC TGCACCTTTC CTTCACCCAG GGATTCTGGC GTACAAAGGC ATGGGGCCCA 
    CCTCTCCTGC AGGCCCCCAC TGGAGCAGAA CTCTGGGTCT GGTTTCAGGA TTCTGTTACT 
    GATGTTAATA AAAGTTGGAA AGAGCTCAGT AACATCTTAT CGGGTATTTT CTGTGCCTCT 
    CTGAACTTCA TCGACTCTAC TAACACAGTT ACTCCGACTG CATCCTTCAA GCCCCTTGGA 
    GTGGCCAACA TTACAGATCA TCACCTGCTC AGATATGCCA CGCTTCCCAG AGAGATAGTT 
    TGCACAGAAA ATCTAACTCC ATGGAAGAAA CTGCTGCCTT GTGGGTCCAA GGCCGGCCTG 
    GCATCTCTAC TGAGGGCTGA ACGCTTGTAT CACAGCAGTT ACCACTCCCA GTCTGTGCAT 
    ATAAGGCCAA TTTGCCAGAA TGCAGATTGT TCAAGTGTCT CCTGGGAGTT AAGGCAGGCC 
    CTTACTGTGG TGTTCGATAT GTATTCTAGT GGCCAGGGAA AAAGAGATTG GTCCATATTT 
    AAAATGTTTT CCCGTACTGT AACTGAGTCG TGTCCTCTGG CATCACAAAG TAAAATCTAT 
    GTTGACGTTT CTGGCTCGGG AAAGGAAGTT GATCTCTTTG AGCTGAACCC TCCTACCTCC 
    TTGGAAAGAC AGGTGGTGGT TCACGGAGAC CGCAGAGTGT ATGCAGAATA CGACCTTTCC 
    CAGCAGGGCA CATTCTCCAC ATCCAGAAAC CTTAATGTAG CCCTTAAGTG GAAAGGGCGG 
    ACCGCTGAGT CAGGCCAGCC ACAGTCCCCC CTCTTTCATG CCGAACGCTA CGTGAGTGGA 
    TATGGGTTGC AGTCTGGAGT GATCAACACT ATGGTATTTA ACAATCACCC GTATAGAGCC 
    TACCCTGTTA TGCTGCTGGA GACAGTGCCT TGGTACCTTC GCCTGTATGT GCACACACTG 
    ACAATCACTA CCAGGGGCAA GGAGAACAAA CCCAGCTACA TTCATTATGA ACCGGCAAAG 
    GATCGCCAGC GCCCACACTT GTTAGAGATG CTGATACAGC TGCCTCCAAA CTCCATTACT 
    AAAATCAGCA TCCAGTTTGA GAGGGCGCTA CTGAAATGGA CGGAATACCC CCCTGATCCT 
    AACCATGGCT TCTACATCAG TCCCTCTGTT CTGAGCGTGC TCATTCCCAG TGTTATTAAC 
    CAGAAGACGG ACGAACTAGA GGAAAGCACA CTGTTCAGCA CTCTGTTTCC ATCATCAGAT 
    GGTTCAAGCT ACTTCATGCG TCTGTACACA GAACCCCTAC TGGTTAACCT TCCCACACCA 
    GACTTCAGCA TGCCCTACAA TGTCATCTGC CTAACTTGCA CTGTGGTGGC CGTGGGCTAC 
    GGCTCTTTTT ATAATCTGCT CACCAGGACA TTCCAGGTTG AAGATAGAAA AGGAGGTGGC 
    CTGGCTAAGA CTATAGCCAA TCTGATACGC AGGTTGCGTG GGGTACCACC TTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    pigt ( XM_012971797.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827251.2 Xenopus tropicalis phosphatidylinositol glycan anchor biosynthesis class T (pigt), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OXa50129
    Clone ID Related Accession (Same CDS sequence) XM_012971797.3
    Accession Version XM_012971797.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1737bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GPI transamidase component PIG-T isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971797.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCCGGCG CTGTGCTTGT AGCCGCTGCT CTGTGGGCCA GCGTTTGGGC CGGGCAGTGT 
    GCCGAGCCGC CTGGGAGTGA TGTCTTCCGA GAGGAGCTGC TCCTCACACC GCTGCGCTCC 
    GGGGACATCG CCGCCACCTT TCAGTTTCGG ACCCGCCTGG ATGCCGACCT GAGGAAGGAG 
    AAGGTCTCCC ACTACAGGAT CTTCCCTAAA GTGCTGGGCC AGCTCATCTC TAAGTACTCC 
    ATTCAGGAGC TGCACCTTTC ATTCACCCAG GGATTCTGGC GTACAAAGGC ATGGGGCCCA 
    CCTCTCCTGC AGGCCCCTAC TGGAGCAGAA CTCTGGGTCT GGTTTCAGGA TTCTGTTACT 
    GATGTTAATA AAAGTTGGAA AGAGCTCAGT AACATCTTAT CGGGTATTTT CTGTGCCTCT 
    CTGAACTTCA TCGACTCTAC TAACACAGTT ACTCCGACTG CATCCTTCAA GCCCCTTGGA 
    GTGGCCAACA TTACAGATCA TCACCTGCTC AGATATGCCA CGCTTCCCAG AGAGATAGTT 
    TGCACAGAAA ATCTAACTCC ATGGAAGAAA CTGCTGCCTT GTGGGTCCAA GGCCGGCCTG 
    GCATCTCTAC TGAGGGCTGA ACGCTTGTAT CACAGCAGTT ACCACTCCCA GTCTGTGCAT 
    ATAAGGCCAA TTTGCCAGAA TGCAGATTGT TCAAGTGTCT CCTGGGAGTT AAGGCAGGCC 
    CTTACTGTGG TGTTCGATAT GTATTCTAGT GGCCAGGGAA AAAGAGATTG GTCCATATTT 
    AAAATGTTTT CCCGTACTGT AACTGAGTCG TGTCCTCTGG CATCACAAAG TAAAATCTAT 
    GTTGACGTTT CTGGCTGGGG AAAGGAAGTT GATCTCTTTG AGCTGAACCC TCCTACCTCC 
    TTGGAAAGAC AGGTGGTGGT TCACGGAGAC CGCAGAGTGT ATGCAGAATA CGACCTTTCC 
    CAGCAGGGCA CATTCTCCAC ATCCAGAAAC CTTAATGTAG CCCTTAAGTG GAAAGGGCGG 
    ACCGCTGAGT CAGGCCAGCC ACAGTCCCCC CTCTTTCATG CCGAACGCTA CGTGAGTGGA 
    TATGGGTTGC AGTCTGGAGT GATCAACACT ATGGTATTTA ACAATCACCC GTATAGAGCC 
    TACCCTGTTA TGCTGCTGGA GACAGTGCCT TGGTACCTTC GCCTGTATGT GCACACACTG 
    ACAATCACTA CCAGGGGCAA GGAGAACAAA CCCAGCTACA TCCATTATGA ACCGGCAAAG 
    GATCGCCAGC GCCCACACTT GTTAGAGATG CTGATACAGC TGCCTCCAAA CTCCATTACT 
    AAAATCAGCA TCCAGTTTGA GAGGGCGCTA CTGAAATGGA CGGAATACCC CCCTGATCCT 
    AACCATGGCT TCTACATCAG TCCCTCTGTT CTGAGCGTGC TCATTCCCAG TGTTATTAAC 
    CAGAAGACGG ACGAACTAGA GGAAAGCACA CTGTTCAGCA CTCTGTTTCC ATCATCAGAT 
    GGTTCAAGCT ACTTCATGCG TCTGTACACA GAACCCCTAC TGGTTAACCT TCCCACACCA 
    GACTTCAGCA TGCCCTACAA TGTCATCTGC CTAACTTGCA CCGTGGTGGC CGTGGGCTAC 
    GGCTCTTTTT ATAATCTGCT CACCAGGACA TTCCAGGTTG AAGATAGAAA AGGAGGTGGC 
    CTGGCTAAGA CTATAGCCAA TCTGATACGC AGGTTGCGTG GGGTACCACC TTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012827251.2
    CDS81..1817
    Translation

    Target ORF information:

    RefSeq Version XM_012971797.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphatidylinositol glycan anchor biosynthesis class T (pigt), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012971797.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCCGGCG CTGTGCTTGT AGCCGCTGCT CTGTGGGCCA GCGTTTGGGC CGGGCAGTGT 
    GCCGAGCCGC CTGGGAGTGA TGTCTTCCGA GAGGAGCTGC TCCTCACACC GCTGCGCTCC 
    GGGGACATCG CCGCCACCTT TCAGTTTCGG ACCCGCCTGG ATGCCGACCT GAGGAAGGAG 
    AAGGTCTCCC ACTACAGGAT CTTCCCTAAA GTGCTGGGCC AGCTCATCTC TAAGTACTCC 
    ATTCAGGAGC TGCACCTTTC ATTCACCCAG GGATTCTGGC GTACAAAGGC ATGGGGCCCA 
    CCTCTCCTGC AGGCCCCTAC TGGAGCAGAA CTCTGGGTCT GGTTTCAGGA TTCTGTTACT 
    GATGTTAATA AAAGTTGGAA AGAGCTCAGT AACATCTTAT CGGGTATTTT CTGTGCCTCT 
    CTGAACTTCA TCGACTCTAC TAACACAGTT ACTCCGACTG CATCCTTCAA GCCCCTTGGA 
    GTGGCCAACA TTACAGATCA TCACCTGCTC AGATATGCCA CGCTTCCCAG AGAGATAGTT 
    TGCACAGAAA ATCTAACTCC ATGGAAGAAA CTGCTGCCTT GTGGGTCCAA GGCCGGCCTG 
    GCATCTCTAC TGAGGGCTGA ACGCTTGTAT CACAGCAGTT ACCACTCCCA GTCTGTGCAT 
    ATAAGGCCAA TTTGCCAGAA TGCAGATTGT TCAAGTGTCT CCTGGGAGTT AAGGCAGGCC 
    CTTACTGTGG TGTTCGATAT GTATTCTAGT GGCCAGGGAA AAAGAGATTG GTCCATATTT 
    AAAATGTTTT CCCGTACTGT AACTGAGTCG TGTCCTCTGG CATCACAAAG TAAAATCTAT 
    GTTGACGTTT CTGGCTGGGG AAAGGAAGTT GATCTCTTTG AGCTGAACCC TCCTACCTCC 
    TTGGAAAGAC AGGTGGTGGT TCACGGAGAC CGCAGAGTGT ATGCAGAATA CGACCTTTCC 
    CAGCAGGGCA CATTCTCCAC ATCCAGAAAC CTTAATGTAG CCCTTAAGTG GAAAGGGCGG 
    ACCGCTGAGT CAGGCCAGCC ACAGTCCCCC CTCTTTCATG CCGAACGCTA CGTGAGTGGA 
    TATGGGTTGC AGTCTGGAGT GATCAACACT ATGGTATTTA ACAATCACCC GTATAGAGCC 
    TACCCTGTTA TGCTGCTGGA GACAGTGCCT TGGTACCTTC GCCTGTATGT GCACACACTG 
    ACAATCACTA CCAGGGGCAA GGAGAACAAA CCCAGCTACA TCCATTATGA ACCGGCAAAG 
    GATCGCCAGC GCCCACACTT GTTAGAGATG CTGATACAGC TGCCTCCAAA CTCCATTACT 
    AAAATCAGCA TCCAGTTTGA GAGGGCGCTA CTGAAATGGA CGGAATACCC CCCTGATCCT 
    AACCATGGCT TCTACATCAG TCCCTCTGTT CTGAGCGTGC TCATTCCCAG TGTTATTAAC 
    CAGAAGACGG ACGAACTAGA GGAAAGCACA CTGTTCAGCA CTCTGTTTCC ATCATCAGAT 
    GGTTCAAGCT ACTTCATGCG TCTGTACACA GAACCCCTAC TGGTTAACCT TCCCACACCA 
    GACTTCAGCA TGCCCTACAA TGTCATCTGC CTAACTTGCA CCGTGGTGGC CGTGGGCTAC 
    GGCTCTTTTT ATAATCTGCT CACCAGGACA TTCCAGGTTG AAGATAGAAA AGGAGGTGGC 
    CTGGCTAAGA CTATAGCCAA TCTGATACGC AGGTTGCGTG GGGTACCACC TTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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