tcirg1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	tcirg1
Entrez Gene ID	549603
Full Name	T cell immune regulator 1, ATPase H+ transporting V0 subunit a3
Synonyms	a3,atp6i,oc116,optb1,tirc7,vph1
General protein information	Preferred Names T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 Names V-type proton ATPase 116 kDa subunit a isoform 3 V-type proton ATPase 116 kDa subunit a T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3 T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031905099.1	XP_031760959.1	V-type proton ATPase 116 kDa subunit a isoform X4
XM_012953599.3	XP_012809053.3	V-type proton ATPase 116 kDa subunit a isoform X1
XM_031905097.1	XP_031760957.1	V-type proton ATPase 116 kDa subunit a isoform X2
XM_031905100.1	XP_031760960.1	V-type proton ATPase 116 kDa subunit a isoform X5
NM_001016849.2	NP_001016849.1	V-type proton ATPase 116 kDa subunit a
XM_031905098.1	XP_031760958.1	V-type proton ATPase 116 kDa subunit a isoform X3
XM_012953599.2	XP_012809053.2	V-type proton ATPase 116 kDa subunit a isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TCIRG1	NP_006010.2
Pan troglodytes (chimpanzee)	TCIRG1	XP_522295.3
Danio rerio (zebrafish)	zgc:55891	NP_998234.1
Danio rerio (zebrafish)	LOC100147923	XP_001922910.4
Xenopus tropicalis (tropical clawed frog)	tcirg1	NP_001016849.1
	TCIRG1	XP_001103418.2
Rattus norvegicus (Norway rat)	Tcirg1	NP_954520.2
Canis lupus familiaris (dog)	TCIRG1	XP_540812.1
Bos taurus (cattle)	TCIRG1	NP_001069102.1
Gallus gallus (chicken)	TCIRG1	NP_990053.1
Danio rerio (zebrafish)	tcirg1	XP_005160329.1
Mus musculus (house mouse)	Tcirg1	NP_001129563.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tcirg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tcirg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa20953	NM_001016849.2 Latest version!	Xenopus tropicalis T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 (tcirg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa45394	XM_012953599.2 Latest version!	Xenopus tropicalis T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 (tcirg1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OXa61458	XM_012953599.3 Latest version!	Xenopus tropicalis T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 (tcirg1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa61459	XM_031905097.1 Latest version!	Xenopus tropicalis T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 (tcirg1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa61460	XM_031905098.1 Latest version!	Xenopus tropicalis T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 (tcirg1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa61461	XM_031905099.1 Latest version!	Xenopus tropicalis T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 (tcirg1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa61462	XM_031905100.1 Latest version!	Xenopus tropicalis T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 (tcirg1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tcirg1 ( NM_001016849.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001016849.1 Xenopus tropicalis T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 (tcirg1), mRNA.

Price & Availability↑

CloneID	OXa20953
Clone ID Related Accession (Same CDS sequence)	NM_001016849.2
Accession Version	NM_001016849.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Xenopus tropicalis(tropical clawed frog)
Product	V-type proton ATPase 116 kDa subunit a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR855432.2. On or before Sep 30, 2006 this sequence version replaced NM_001015854.1, NM_001016849.1. ##Evidence-Data-START## Transcript exon combination :: CR855432.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGGGCTCGC TATTTCGGGG AGAAGAGATG TGCCTAGCAC AGTTATTTCT GCAGTCTGGT 
AGTGAGTATC AATGTGTTAG TGCACTGGGA GAACTTGGAC TAGTGGAATT CCGGGATCTC 
AATCAAAATG TCAATTCCTT TCAGCGCCGA TATGTATCAG AGATAAGACG TTGTGATGAA 
ATGGAGACAA CATTCTCATA CCTAGAACGA GAATTGCGTA AAGCTGGAGT ACAAGTTCCT 
GAGTCTGAAA TGTCTCCGCC TGCTCCCTTA CCGAGAGATG CCATCAGAAT GCAAGAAGAA 
TCTGAGCAGC TGGCAAAAGA GCTTAGAGAG GTGTCTCAAA ATCGACAGAC TCTACAAGAG 
CGTTTGAGAG AGCTACTGGA ATATGCCAAT ATCCTTCGGG AGAGCCAGAG ATTTACTGGG 
CCACTGCTGG AGTCTGAAGC ACAATGGAAG GACAGGTCTG AGGATGATCC ACTGCTGGAC 
CCTGCTGTAG TCAATAAACA AGACCTCAGA GTCAGCTTTA TGGCAGGTGT TATTCACCCA 
TGGCGCATGA ACTCATTTGA ACGTTTGTTG TGGCGGGCTT GCCGTGGTTA CCTGATTGTC 
AACTTTGTAG AAATGTCAGA GCCAATGGAA GATTTGGTGA CTGGTGAGAG TGTCACTCAA 
ATTATATTTC TAATTTCCTA CTGGGGCGAA AAAATTGGAG AGAAGATCAA AAAGATTGCA 
AACTGTTTTC ACTGTCATAT TTATCCGTAT GCTGATGATG AAACTTCACG CCTTGAGACA 
CTAAGAAATC TTCTGGTTCA GATTCAAGAC ATGCAAAAAG TGTTGTTACA GACAGAAGGT 
TACCTCTCAC AAGTACTTTC CCGAGCTGCA TCAGCTCTGC ACCACTGGAG AGTTAGTGTG 
CGAAAGATGA AACATATTTA TCTCATTCTG AACTTATGCA GTGTACGGGA GCGTTGCCTG 
ATTGGTGAAG TGTGGTGTCC TGTGGTTGAT CTTCCCCTTC TTCAAAGTGC ACTTACCCGG 
GCGTCGGAGA GCAGTGGAGG CGGTGGAGAG TCTTTCTGTC ATCGAATACC TTGTGCTTTT 
AGCCCTCCAA CTCTTATTCG TACCAATAAA TTCACTTCAG GTTTCCAGGG AATTGTTGAT 
GCTTATGGAG TAGCCACATA TCAGGAGGTG AATCCAGCTA TCTTCTCAAT CATAACGTTT 
CCATTCCTGT TTGCTGTGAT GTTTGGGGAT GTGGGACATG GTGCGATCAT GTTCCTCTTT 
GCTCTCTGGC TGGTTCTTGG TGAGAATGAT CCAAAACTTA GACGTTCTGA AGATGAGATA 
TTTTCAATGT GTTTCGGGGG GCGTTACCTT ATCCTGCTGA TGGGAGCACT CTCGGTATAC 
ACAGGGTTTG TGTATAATGA GTGTTTCAGT CGACCAGCTG TCATTTTTAA CTCAGGATGG 
AGTGTTGCTT CTATGGCAAG AGCCAACAAC TGGACGTCAG ACTCAATAAA TAAATTGCCT 
CCAATTCCTC TGAACCCCAA CATTACTGGT GTGTTTACTG CACCATATCC ATTTGGCATT 
GATCCTATCT GGAGTCTTGC AGTAAATCGA CTTACTTTCT TGAACTCCTT CAAGATGAAA 
ATGTCTGTTA TTTTGGGTGT TTGTCACATG GCATTTGGTG TCTGCCTAAG TGTCTTTAAC 
TTTGTTCACT TTAGGCAAAT ATACCGCATT TTCCTCATAA CCCTTCCGGA ATTGTTGTTT 
CTACTTTGTC TCTTTGGGTA CCTGGTTTTT ATGGTGGTGT ATAAATGGAT TGTGCTGACA 
GCTGAGGATG CTGAGTGGGC TCCAAGTATA CTTATACATT TTATAGACAT GTTCCTGTTT 
ACTCAGAACC CTGGAAACAG AGATTTGTAC CAGGGGCAGC AAGTTGTGCA GACAGTTTTA 
GTGATTGTTG CTATACTATG CATCCCAGTT CTGTTGCTGG GGGATCCAAT ATGTCTCCTT 
ATTCAGCATC GGAGTAAAAA AGACAGCTCT CATCAGAATC GGTGCTCGCT GAATAATGGG 
GATCGAGAAG CTCTACTGGA AGATGAGATT ACAGTACCCA CAGGACATGG ACATGGCAGT 
GAAAAGTTTG ATACAGCTGA AGTTTTCATG CATCAAATGA TTCATACTAT AGAGTACTGT 
CTGGGCTGCA TATCTAATAC AGCCTCTTAC CTTCGACTGT GGGCGCTCAG CTTGGCACAT 
GCACAGCTGT CTGAGGTCCT GTGGAATATG GTGATTAGAA TTGGATTCTC CAAGTTATCC 
TTAACATGGG GCATTGTTCT TGTTCCAATC TTTGCTTTTT TTGCTGTCCT GACAGTCGCC 
ATTTTGCTTA TTATGGAGGG ACTGTCTGCT TTTTTACATG CTCTCCGTCT GCACTGGGTG 
GAGTTCCAAA ACAAATTTTA TTCTGGTGAA GGATACAAAT TCTTTCCCTT TTGCTTTGAG 
ACAATGTATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001016849.1
CDS	39..2510
Translation	MGSLFRGEEMCLAQLFLQSGSEYQCVSALGELGLVEFRDLNQNVNSFQRRYVSEIRRCDEMETTFSYLERELRKAGVQVPESEMSPPAPLPRDAIRMQEESEQLAKELREVSQNRQTLQERLRELLEYANILRESQRFTGPLLESEAQWKDRSEDDPLLDPAVVNKQDLRVSFMAGVIHPWRMNSFERLLWRACRGYLIVNFVEMSEPMEDLVTGESVTQIIFLISYWGEKIGEKIKKIANCFHCHIYPYADDETSRLETLRNLLVQIQDMQKVLLQTEGYLSQVLSRAASALHHWRVSVRKMKHIYLILNLCSVRERCLIGEVWCPVVDLPLLQSALTRASESSGGGGESFCHRIPCAFSPPTLIRTNKFTSGFQGIVDAYGVATYQEVNPAIFSIITFPFLFAVMFGDVGHGAIMFLFALWLVLGENDPKLRRSEDEIFSMCFGGRYLILLMGALSVYTGFVYNECFSRPAVIFNSGWSVASMARANNWTSDSINKLPPIPLNPNITGVFTAPYPFGIDPIWSLAVNRLTFLNSFKMKMSVILGVCHMAFGVCLSVFNFVHFRQIYRIFLITLPELLFLLCLFGYLVFMVVYKWIVLTAEDAEWAPSILIHFIDMFLFTQNPGNRDLYQGQQVVQTVLVIVAILCIPVLLLGDPICLLIQHRSKKDSSHQNRCSLNNGDREALLEDEITVPTGHGHGSEKFDTAEVFMHQMIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWNMVIRIGFSKLSLTWGIVLVPIFAFFAVLTVAILLIMEGLSAFLHALRLHWVEFQNKFYSGEGYKFFPFCFETMY

Target ORF information:

RefSeq Version	NM_001016849.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 (tcirg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001016849.2

ORF Insert Sequence:

ATGGGCTCGC TATTTCGGGG AGAAGAGATG TGCCTAGCAC AGTTATTTCT GCAGTCTGGT 
AGTGAGTATC AATGTGTTAG TGCACTGGGA GAACTTGGAC TAGTGGAATT CCGGGATCTC 
AATCAAAATG TCAATTCCTT TCAGCGCCGA TATGTATCAG AGATAAGACG TTGTGATGAA 
ATGGAGACAA CATTCTCATA CCTAGAACGA GAATTGCGTA AAGCTGGAGT ACAAGTTCCT 
GAGTCTGAAA TGTCTCCGCC TGCTCCCTTA CCGAGAGATG CCATCAGAAT GCAAGAAGAA 
TCTGAGCAGC TGGCAAAAGA GCTTAGAGAG GTGTCTCAAA ATCGACAGAC TCTACAAGAG 
CGTTTGAGAG AGCTACTGGA ATATGCCAAT ATCCTTCGGG AGAGCCAGAG ATTTACTGGG 
CCACTGCTGG AGTCTGAAGC ACAATGGAAG GACAGGTCTG AGGATGATCC ACTGCTGGAC 
CCTGCTGTAG TCAATAAACA AGACCTCAGA GTCAGCTTTA TGGCAGGTGT TATTCACCCA 
TGGCGCATGA ACTCATTTGA ACGTTTGTTG TGGCGGGCTT GCCGTGGTTA CCTGATTGTC 
AACTTTGTAG AAATGTCAGA GCCAATGGAA GATTTGGTGA CTGGTGAGAG TGTCACTCAA 
ATTATATTTC TAATTTCCTA CTGGGGCGAA AAAATTGGAG AGAAGATCAA AAAGATTGCA 
AACTGTTTTC ACTGTCATAT TTATCCGTAT GCTGATGATG AAACTTCACG CCTTGAGACA 
CTAAGAAATC TTCTGGTTCA GATTCAAGAC ATGCAAAAAG TGTTGTTACA GACAGAAGGT 
TACCTCTCAC AAGTACTTTC CCGAGCTGCA TCAGCTCTGC ACCACTGGAG AGTTAGTGTG 
CGAAAGATGA AACATATTTA TCTCATTCTG AACTTATGCA GTGTACGGGA GCGTTGCCTG 
ATTGGTGAAG TGTGGTGTCC TGTGGTTGAT CTTCCCCTTC TTCAAAGTGC ACTTACCCGG 
GCGTCGGAGA GCAGTGGAGG CGGTGGAGAG TCTTTCTGTC ATCGAATACC TTGTGCTTTT 
AGCCCTCCAA CTCTTATTCG TACCAATAAA TTCACTTCAG GTTTCCAGGG AATTGTTGAT 
GCTTATGGAG TAGCCACATA TCAGGAGGTG AATCCAGCTA TCTTCTCAAT CATAACGTTT 
CCATTCCTGT TTGCTGTGAT GTTTGGGGAT GTGGGACATG GTGCGATCAT GTTCCTCTTT 
GCTCTCTGGC TGGTTCTTGG TGAGAATGAT CCAAAACTTA GACGTTCTGA AGATGAGATA 
TTTTCAATGT GTTTCGGGGG GCGTTACCTT ATCCTGCTGA TGGGAGCACT CTCGGTATAC 
ACAGGGTTTG TGTATAATGA GTGTTTCAGT CGACCAGCTG TCATTTTTAA CTCAGGATGG 
AGTGTTGCTT CTATGGCAAG AGCCAACAAC TGGACGTCAG ACTCAATAAA TAAATTGCCT 
CCAATTCCTC TGAACCCCAA CATTACTGGT GTGTTTACTG CACCATATCC ATTTGGCATT 
GATCCTATCT GGAGTCTTGC AGTAAATCGA CTTACTTTCT TGAACTCCTT CAAGATGAAA 
ATGTCTGTTA TTTTGGGTGT TTGTCACATG GCATTTGGTG TCTGCCTAAG TGTCTTTAAC 
TTTGTTCACT TTAGGCAAAT ATACCGCATT TTCCTCATAA CCCTTCCGGA ATTGTTGTTT 
CTACTTTGTC TCTTTGGGTA CCTGGTTTTT ATGGTGGTGT ATAAATGGAT TGTGCTGACA 
GCTGAGGATG CTGAGTGGGC TCCAAGTATA CTTATACATT TTATAGACAT GTTCCTGTTT 
ACTCAGAACC CTGGAAACAG AGATTTGTAC CAGGGGCAGC AAGTTGTGCA GACAGTTTTA 
GTGATTGTTG CTATACTATG CATCCCAGTT CTGTTGCTGG GGGATCCAAT ATGTCTCCTT 
ATTCAGCATC GGAGTAAAAA AGACAGCTCT CATCAGAATC GGTGCTCGCT GAATAATGGG 
GATCGAGAAG CTCTACTGGA AGATGAGATT ACAGTACCCA CAGGACATGG ACATGGCAGT 
GAAAAGTTTG ATACAGCTGA AGTTTTCATG CATCAAATGA TTCATACTAT AGAGTACTGT 
CTGGGCTGCA TATCTAATAC AGCCTCTTAC CTTCGACTGT GGGCGCTCAG CTTGGCACAT 
GCACAGCTGT CTGAGGTCCT GTGGAATATG GTGATTAGAA TTGGATTCTC CAAGTTATCC 
TTAACATGGG GCATTGTTCT TGTTCCAATC TTTGCTTTTT TTGCTGTCCT GACAGTCGCC 
ATTTTGCTTA TTATGGAGGG ACTGTCTGCT TTTTTACATG CTCTCCGTCT GCACTGGGTG 
GAGTTCCAAA ACAAATTTTA TTCTGGTGAA GGATACAAAT TCTTTCCCTT TTGCTTTGAG 
ACAATGTATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tcirg1 ( XM_012953599.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809053.2 Xenopus tropicalis T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 (tcirg1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa45394
Clone ID Related Accession (Same CDS sequence)	XM_012953599.2
Accession Version	XM_012953599.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	V-type proton ATPase 116 kDa subunit a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012953599.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCGC TATTTCGGGG AGAAGAGATG TGCCTAGCAC AGTTATTTCT GCAGTCTGGT 
AGTGAGTATC AATGTGTTAG TGCACTGGGA GAACTTGGAC TAGTGGAATT CCGGGATCTC 
AATCAAAATG TCAATTCCTT TCAGCGCCGA TATGTATCAG AGATAAGACG TTGTGATGAA 
ATGGAGACAA CATTCTCATA CCTAGAACGA GAATTGCGTA AAGCTGGAGT ACAAGTTCCT 
GAGTCTGAAA TGTCTCCGCC TGCTCCCTTA CCGAGAGATG CCATCAGAAT GCAAGAAGAA 
TCTGAGCAGC TGGCAAAAGA GCTTAGAGAG GTGTCTCAAA ATCGACAGAC TCTACAAGAG 
CGTTTGAGAG AGCTACTGGA ATATGCCAAT ATCCTTCGGG AGAGCCAGAG ATTTACTGGG 
CCACTGCTGG AGTCTGAAGC ACAATGGAAG GACAGGTCTG AGGATGATCC ACTGCTGGAC 
CCTGCTGTAG TCAATAAACA AGACCTCAGA GTCAGCTTTA TGGCAGGTGT TATTCACCCA 
TGGCGCATGA ACTCATTTGA ACGTTTGTTG TGGCGGGCTT GCCGTGGTTA CCTGATTGTC 
AACTTTGTAG AAATGTCAGA GCCAATGGAA GATTTGGTGA CTGGTGAGAG TGTCACTCAA 
ATTATATTTC TAATTTCCTA CTGGGGCGAA AAAATTGGAG AGAAGATCAA AAAGATTGCA 
AACTGTTTTC ACTGTCATAT TTATCCGTAT GCTGATGATG AAACTTCACG CCTTGAGACA 
CTAAGAAATC TTCTGGTTCA GATTCAAGAC ATGCAAAAAG TGTTGTTACA GACAGAAGGT 
TACCTCTCAC AAGTACTTTC CCGAGCTGCA TCAGCTCTGC ACCACTGGAG AGTTAGTGTG 
CGAAAGATGA AACATATTTA TCTCATTCTG AACTTATGCA GTGTACGGGA GCGTTGCCTG 
ATTGGTGAAG TGTGGTGTCC TGTGGTTGAT CTTCCCCTTC TTCAAAGTGC ACTTACCCGG 
GCGTCGGAGA GCAGTGGAGG CGGTGGAGAG TCTTTCTGTC ATCGAATACC TTGTGCTTTT 
AGCCCTCCAA CTCTTATTCG TACCAATAAA TTCACTTCAG GTTTCCAGGG AATTGTTGAT 
GCTTATGGAG TAGCCACATA TCAGGAGGTG AATCCAGCTA TCTTCTCAAT CATAACGTTT 
CCATTCCTGT TTGCTGTGAT GTTTGGGGAT GTGGGACATG GTGCGATCAT GTTCCTCTTT 
GCTCTCTGGC TGGTTCTTGG TGAGAATGAT CCAAAACTTA GACGTTCTGA AGATGAGATA 
TTTTCAATGT GTTTCGGGGG GCGTTACCTT ATCCTGCTGA TGGGAGCACT CTCGGTATAC 
ACAGGGTTTG TGTATAATGA GTGTTTCAGT CGACCAGCTG TCATTTTTAA CTCAGGATGG 
AGTGTTGCTT CTATGGCAAG AGCCAACAAC TGGACGTCAG ACTCAATAAA TAAATTGCCT 
CCAATTCCTC TGAACCCCAA CATTACTGGT GTGTTTACTG CACCATATCC ATTTGGCATT 
GATCCTATCT GGAGTCTTGC AGTAAATCGA CTTACTTTCT TGAACTCCTT CAAGATGAAA 
ATGTCTGTTA TTTTGGGTGT TTGTCACATG GCATTTGGTG TCTGCCTAAG TGTCTTTAAC 
TTTGTTCACT TTAGGCAAAT ATACCGCATT TTCCTCATAA CCCTTCCGGA ATTGTTGTTT 
CTACTTTGTC TCTTTGGGTA CCTGGTTTTT ATGGTGGTGT ATAAATGGAT TGTGCTGACA 
GCTGAGGATG CTGAGTGGGC TCCAAGTATA CTTATACATT TTATAGACAT GTTCCTGTTT 
ACTCAGAACC CTGGAAACAG AGATTTGTAC CAGGGGCAGC AAGTTGTGCA GACAGTTTTA 
GTGATTGTTG CTATACTATG CATCCCAGTT CTGTTGCTGG GGGATCCAAT ATGTCTCCTT 
ATTCAGCATC GGAGTAAAAA AGACAGCTCT CATCAGAATC GGTGCTCGCT GAATAATGGG 
GATCGAGAAG CTCTACTGGA AGATGAGATT ACAGTACCCA CAGGACATGG ACATGGCAGT 
GAAAAGTTTG ATACAGCTGA AGTTTTCATG CATCAAATGA TTCATACTAT AGAGTACTGT 
CTGGGCTGCA TATCTAATAC AGCCTCTTAC CTTCGACTGT GGGCGCTCAG CTTGGCACAT 
GCACAGCTGT CTGAGGTCCT GTGGAATATG GTGATTAGAA TTGGATTCTC CAAGTTATCC 
TTAACATGGG GCATTGTTCT TGTTCCAATC TTTGCTTTTT TTGCTGTCCT GACAGTCGCC 
ATTTTGCTTA TTATGGAGGG ACTGTCTGCT TTTTTACATG CTCTCCGTCT GCACTGGGGG 
GAGTTCCAAA ACAAATTTTA TTCTGGGGAA GGAAACAAAT TCTTTCCCTT TTGCTTTGAG 
ACAATGTATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809053.2
CDS	126..2597
Translation	MGSLFRGEEMCLAQLFLQSGSEYQCVSALGELGLVEFRDLNQNVNSFQRRYVSEIRRCDEMETTFSYLERELRKAGVQVPESEMSPPAPLPRDAIRMQEESEQLAKELREVSQNRQTLQERLRELLEYANILRESQRFTGPLLESEAQWKDRSEDDPLLDPAVVNKQDLRVSFMAGVIHPWRMNSFERLLWRACRGYLIVNFVEMSEPMEDLVTGESVTQIIFLISYWGEKIGEKIKKIANCFHCHIYPYADDETSRLETLRNLLVQIQDMQKVLLQTEGYLSQVLSRAASALHHWRVSVRKMKHIYLILNLCSVRERCLIGEVWCPVVDLPLLQSALTRASESSGGGGESFCHRIPCAFSPPTLIRTNKFTSGFQGIVDAYGVATYQEVNPAIFSIITFPFLFAVMFGDVGHGAIMFLFALWLVLGENDPKLRRSEDEIFSMCFGGRYLILLMGALSVYTGFVYNECFSRPAVIFNSGWSVASMARANNWTSDSINKLPPIPLNPNITGVFTAPYPFGIDPIWSLAVNRLTFLNSFKMKMSVILGVCHMAFGVCLSVFNFVHFRQIYRIFLITLPELLFLLCLFGYLVFMVVYKWIVLTAEDAEWAPSILIHFIDMFLFTQNPGNRDLYQGQQVVQTVLVIVAILCIPVLLLGDPICLLIQHRSKKDSSHQNRCSLNNGDREALLEDEITVPTGHGHGSEKFDTAEVFMHQMIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWNMVIRIGFSKLSLTWGIVLVPIFAFFAVLTVAILLIMEGLSAFLHALRLHWGEFQNKFYSGEGNKFFPFCFETMY

Target ORF information:

RefSeq Version	XM_012953599.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 (tcirg1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953599.2

ORF Insert Sequence:

ATGGGCTCGC TATTTCGGGG AGAAGAGATG TGCCTAGCAC AGTTATTTCT GCAGTCTGGT 
AGTGAGTATC AATGTGTTAG TGCACTGGGA GAACTTGGAC TAGTGGAATT CCGGGATCTC 
AATCAAAATG TCAATTCCTT TCAGCGCCGA TATGTATCAG AGATAAGACG TTGTGATGAA 
ATGGAGACAA CATTCTCATA CCTAGAACGA GAATTGCGTA AAGCTGGAGT ACAAGTTCCT 
GAGTCTGAAA TGTCTCCGCC TGCTCCCTTA CCGAGAGATG CCATCAGAAT GCAAGAAGAA 
TCTGAGCAGC TGGCAAAAGA GCTTAGAGAG GTGTCTCAAA ATCGACAGAC TCTACAAGAG 
CGTTTGAGAG AGCTACTGGA ATATGCCAAT ATCCTTCGGG AGAGCCAGAG ATTTACTGGG 
CCACTGCTGG AGTCTGAAGC ACAATGGAAG GACAGGTCTG AGGATGATCC ACTGCTGGAC 
CCTGCTGTAG TCAATAAACA AGACCTCAGA GTCAGCTTTA TGGCAGGTGT TATTCACCCA 
TGGCGCATGA ACTCATTTGA ACGTTTGTTG TGGCGGGCTT GCCGTGGTTA CCTGATTGTC 
AACTTTGTAG AAATGTCAGA GCCAATGGAA GATTTGGTGA CTGGTGAGAG TGTCACTCAA 
ATTATATTTC TAATTTCCTA CTGGGGCGAA AAAATTGGAG AGAAGATCAA AAAGATTGCA 
AACTGTTTTC ACTGTCATAT TTATCCGTAT GCTGATGATG AAACTTCACG CCTTGAGACA 
CTAAGAAATC TTCTGGTTCA GATTCAAGAC ATGCAAAAAG TGTTGTTACA GACAGAAGGT 
TACCTCTCAC AAGTACTTTC CCGAGCTGCA TCAGCTCTGC ACCACTGGAG AGTTAGTGTG 
CGAAAGATGA AACATATTTA TCTCATTCTG AACTTATGCA GTGTACGGGA GCGTTGCCTG 
ATTGGTGAAG TGTGGTGTCC TGTGGTTGAT CTTCCCCTTC TTCAAAGTGC ACTTACCCGG 
GCGTCGGAGA GCAGTGGAGG CGGTGGAGAG TCTTTCTGTC ATCGAATACC TTGTGCTTTT 
AGCCCTCCAA CTCTTATTCG TACCAATAAA TTCACTTCAG GTTTCCAGGG AATTGTTGAT 
GCTTATGGAG TAGCCACATA TCAGGAGGTG AATCCAGCTA TCTTCTCAAT CATAACGTTT 
CCATTCCTGT TTGCTGTGAT GTTTGGGGAT GTGGGACATG GTGCGATCAT GTTCCTCTTT 
GCTCTCTGGC TGGTTCTTGG TGAGAATGAT CCAAAACTTA GACGTTCTGA AGATGAGATA 
TTTTCAATGT GTTTCGGGGG GCGTTACCTT ATCCTGCTGA TGGGAGCACT CTCGGTATAC 
ACAGGGTTTG TGTATAATGA GTGTTTCAGT CGACCAGCTG TCATTTTTAA CTCAGGATGG 
AGTGTTGCTT CTATGGCAAG AGCCAACAAC TGGACGTCAG ACTCAATAAA TAAATTGCCT 
CCAATTCCTC TGAACCCCAA CATTACTGGT GTGTTTACTG CACCATATCC ATTTGGCATT 
GATCCTATCT GGAGTCTTGC AGTAAATCGA CTTACTTTCT TGAACTCCTT CAAGATGAAA 
ATGTCTGTTA TTTTGGGTGT TTGTCACATG GCATTTGGTG TCTGCCTAAG TGTCTTTAAC 
TTTGTTCACT TTAGGCAAAT ATACCGCATT TTCCTCATAA CCCTTCCGGA ATTGTTGTTT 
CTACTTTGTC TCTTTGGGTA CCTGGTTTTT ATGGTGGTGT ATAAATGGAT TGTGCTGACA 
GCTGAGGATG CTGAGTGGGC TCCAAGTATA CTTATACATT TTATAGACAT GTTCCTGTTT 
ACTCAGAACC CTGGAAACAG AGATTTGTAC CAGGGGCAGC AAGTTGTGCA GACAGTTTTA 
GTGATTGTTG CTATACTATG CATCCCAGTT CTGTTGCTGG GGGATCCAAT ATGTCTCCTT 
ATTCAGCATC GGAGTAAAAA AGACAGCTCT CATCAGAATC GGTGCTCGCT GAATAATGGG 
GATCGAGAAG CTCTACTGGA AGATGAGATT ACAGTACCCA CAGGACATGG ACATGGCAGT 
GAAAAGTTTG ATACAGCTGA AGTTTTCATG CATCAAATGA TTCATACTAT AGAGTACTGT 
CTGGGCTGCA TATCTAATAC AGCCTCTTAC CTTCGACTGT GGGCGCTCAG CTTGGCACAT 
GCACAGCTGT CTGAGGTCCT GTGGAATATG GTGATTAGAA TTGGATTCTC CAAGTTATCC 
TTAACATGGG GCATTGTTCT TGTTCCAATC TTTGCTTTTT TTGCTGTCCT GACAGTCGCC 
ATTTTGCTTA TTATGGAGGG ACTGTCTGCT TTTTTACATG CTCTCCGTCT GCACTGGGGG 
GAGTTCCAAA ACAAATTTTA TTCTGGGGAA GGAAACAAAT TCTTTCCCTT TTGCTTTGAG 
ACAATGTATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tcirg1 ( XM_012953599.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809053.3 Xenopus tropicalis T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 (tcirg1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa61458
Clone ID Related Accession (Same CDS sequence)	XM_012953599.3
Accession Version	XM_012953599.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	V-type proton ATPase 116 kDa subunit a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012953599.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGCC CTCTCTTTGC TTACTTCCCC ATTCCTCCAC CCACACTGTT GATACACAAG 
AAAGAGGAAG TGCCTTTGCT TGTGTTGTTG GAAAGTCGGC AGAAAGGTTT GTGCTTTTTC 
CTGAAAATGG GCTCGCTATT TCGGGGAGAA GAGATGTGCC TAGCACAGTT ATTTCTGCAG 
TCTGGTAGTG AGTATCAATG TGTTAGTGCA CTGGGAGAAC TTGGACTAGT GGAATTCCGG 
GATCTCAATC AAAATGTCAA TTCCTTTCAG CGCCGATATG TATCAGAGAT AAGACGTTGT 
GATGAAATGG AGACAACATT CTCATACCTA GAACGAGAAT TGCGTAAAGC TGGAGTACAA 
GTTCCTGAGT CTGAAATGTC TCCGCCTGCT CCCTTACCGA GAGATGCCAT CAGAATGCAA 
GAAGAATCTG AGCAGCTGGC AAAAGAGCTT AGAGAGGTGT CTCAAAATCG ACAGACTCTA 
CAAGAGCGTT TGAGAGAGCT ACTGGAATAT GCCAATATCC TTCGGGAGAG CCAGAGATTT 
ACTGGGCCAC TGCTGGAGTC TGAAGCACAA TGGAAGGACA GGTCTGAGGA TGATCCACTG 
CTGGACCCTG CTGTAGTCAA TAAACAAGAC CTCAGAGTCA GCTTTATGGC AGGTGTTATT 
CACCCATGGC GCATGAACTC ATTTGAACGT TTGTTGTGGC GGGCTTGCCG TGGTTACCTG 
ATTGTCAACT TTGTAGAAAT GTCAGAGCCA ATGGAAGATT TGGTGACTGG TGAGAGTGTC 
ACTCAAATTA TATTTCTAAT TTCCTACTGG GGCGAAAAAA TTGGAGAGAA GATCAAAAAG 
ATTGCAAACT GTTTTCACTG TCATATTTAT CCGTATGCTG ATGATGAAAC TTCACGCCTT 
GAGACACTAA GAAATCTTCT GGTTCAGATT CAAGACATGC AAAAAGTGTT GTTACAGACA 
GAAGGTTACC TCTCACAAGT ACTTTCCCGA GCTGCATCAG CTCTGCACCA CTGGAGAGTT 
AGTGTGCGAA AGATGAAACA TATTTATCTC ATTCTGAACT TATGCAGTGT ACGGGAGCGT 
TGCCTGATTG GTGAAGTGTG GTGTCCTGTG GTTGATCTTC CCCTTCTTCA AAGTGCACTT 
ACCCGGGCGT CGGAGAGCAG TGGAGGCGGT GGAGAGTCTT TCTGTCATCG AATACCTTGT 
GCTTTTAGCC CTCCAACTCT TATTCGTACC AATAAATTCA CTTCAGGTTT CCAGGGAATT 
GTTGATGCTT ATGGAGTAGC CACATATCAG GAGGTGAATC CAGCTATCTT CTCAATCATA 
ACGTTTCCAT TCCTGTTTGC TGTGATGTTT GGGGATGTGG GACATGGTGC GATCATGTTC 
CTCTTTGCTC TCTGGCTGGT TCTTGGTGAG AATGATCCAA AACTTAGACG TTCTGAAGAT 
GAGATATTTT CAATGTGTTT CGGGGGGCGT TACCTTATCC TGCTGATGGG AGCACTCTCG 
GTATACACAG GGTTTGTGTA TAATGAGTGT TTCAGTCGAC CAGCTGTCAT TTTTAACTCA 
GGATGGAGTG TTGCTTCTAT GGCAAGAGCC AACAACTGGA CGTCAGACTC AATAAATAAA 
TTGCCTCCAA TTCCTCTGAA CCCCAACATT ACTGGTGTGT TTACTGCACC ATATCCATTT 
GGCATTGATC CTATCTGGAG TCTTGCAGTA AATCGACTTA CTTTCTTGAA CTCCTTCAAG 
ATGAAAATGT CTGTTATTTT GGGTGTTTGT CACATGGCAT TTGGTGTCTG CCTAAGTGTC 
TTTAACTTTG TTCACTTTAG GCAAATATAC CGCATTTTCC TCATAACCCT TCCGGAATTG 
TTGTTTCTAC TTTGTCTCTT TGGGTACCTG GTTTTTATGG TGGTGTATAA ATGGATTGTG 
CTGACAGCTG AGGATGCTGA GTGGGCTCCA AGTATACTTA TACATTTTAT AGACATGTTC 
CTGTTTACTC AGAACCCTGG AAACAGAGAT TTGTACCAGG GGCAGCAAGT TGTGCAGACA 
GTTTTAGTGA TTGTTGCTAT ACTATGCATC CCAGTTCTGT TGCTGGGGGA TCCAATATGT 
CTCCTTATTC AGCATCGGAG TAAAAAAGAC AGCTCTCATC AGAATCGGTG CTCGCTGAAT 
AATGGGGATC GAGAAGCTCT ACTGGAAGAT GAGATTACAG TACCCACAGG ACATGGACAT 
GGCAGTGAAA AGTTTGATAC AGCTGAAGTT TTCATGCATC AAATGATTCA TACTATAGAG 
TACTGTCTGG GCTGCATATC TAATACAGCC TCTTACCTTC GACTGTGGGC GCTCAGCTTG 
GCACATGCAC AGCTGTCTGA GGTCCTGTGG AATATGGTGA TTAGAATTGG ATTCTCCAAG 
TTATCCTTAA CATGGGGCAT TGTTCTTGTT CCAATCTTTG CTTTTTTTGC TGTCCTGACA 
GTCGCCATTT TGCTTATTAT GGAGGGACTG TCTGCTTTTT TACATGCTCT CCGTCTGCAC 
TGGGTGGAGT TCCAAAACAA ATTTTATTCT GGTGAAGGAT ACAAATTCTT TCCCTTTTGC 
TTTGAGACAA TGTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809053.3
CDS	16..2613
Translation	MARPLFAYFPIPPPTLLIHKKEEVPLLVLLESRQKGLCFFLKMGSLFRGEEMCLAQLFLQSGSEYQCVSALGELGLVEFRDLNQNVNSFQRRYVSEIRRCDEMETTFSYLERELRKAGVQVPESEMSPPAPLPRDAIRMQEESEQLAKELREVSQNRQTLQERLRELLEYANILRESQRFTGPLLESEAQWKDRSEDDPLLDPAVVNKQDLRVSFMAGVIHPWRMNSFERLLWRACRGYLIVNFVEMSEPMEDLVTGESVTQIIFLISYWGEKIGEKIKKIANCFHCHIYPYADDETSRLETLRNLLVQIQDMQKVLLQTEGYLSQVLSRAASALHHWRVSVRKMKHIYLILNLCSVRERCLIGEVWCPVVDLPLLQSALTRASESSGGGGESFCHRIPCAFSPPTLIRTNKFTSGFQGIVDAYGVATYQEVNPAIFSIITFPFLFAVMFGDVGHGAIMFLFALWLVLGENDPKLRRSEDEIFSMCFGGRYLILLMGALSVYTGFVYNECFSRPAVIFNSGWSVASMARANNWTSDSINKLPPIPLNPNITGVFTAPYPFGIDPIWSLAVNRLTFLNSFKMKMSVILGVCHMAFGVCLSVFNFVHFRQIYRIFLITLPELLFLLCLFGYLVFMVVYKWIVLTAEDAEWAPSILIHFIDMFLFTQNPGNRDLYQGQQVVQTVLVIVAILCIPVLLLGDPICLLIQHRSKKDSSHQNRCSLNNGDREALLEDEITVPTGHGHGSEKFDTAEVFMHQMIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWNMVIRIGFSKLSLTWGIVLVPIFAFFAVLTVAILLIMEGLSAFLHALRLHWVEFQNKFYSGEGYKFFPFCFETMY

Target ORF information:

RefSeq Version	XM_012953599.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 (tcirg1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953599.3

ORF Insert Sequence:

ATGGCCCGCC CTCTCTTTGC TTACTTCCCC ATTCCTCCAC CCACACTGTT GATACACAAG 
AAAGAGGAAG TGCCTTTGCT TGTGTTGTTG GAAAGTCGGC AGAAAGGTTT GTGCTTTTTC 
CTGAAAATGG GCTCGCTATT TCGGGGAGAA GAGATGTGCC TAGCACAGTT ATTTCTGCAG 
TCTGGTAGTG AGTATCAATG TGTTAGTGCA CTGGGAGAAC TTGGACTAGT GGAATTCCGG 
GATCTCAATC AAAATGTCAA TTCCTTTCAG CGCCGATATG TATCAGAGAT AAGACGTTGT 
GATGAAATGG AGACAACATT CTCATACCTA GAACGAGAAT TGCGTAAAGC TGGAGTACAA 
GTTCCTGAGT CTGAAATGTC TCCGCCTGCT CCCTTACCGA GAGATGCCAT CAGAATGCAA 
GAAGAATCTG AGCAGCTGGC AAAAGAGCTT AGAGAGGTGT CTCAAAATCG ACAGACTCTA 
CAAGAGCGTT TGAGAGAGCT ACTGGAATAT GCCAATATCC TTCGGGAGAG CCAGAGATTT 
ACTGGGCCAC TGCTGGAGTC TGAAGCACAA TGGAAGGACA GGTCTGAGGA TGATCCACTG 
CTGGACCCTG CTGTAGTCAA TAAACAAGAC CTCAGAGTCA GCTTTATGGC AGGTGTTATT 
CACCCATGGC GCATGAACTC ATTTGAACGT TTGTTGTGGC GGGCTTGCCG TGGTTACCTG 
ATTGTCAACT TTGTAGAAAT GTCAGAGCCA ATGGAAGATT TGGTGACTGG TGAGAGTGTC 
ACTCAAATTA TATTTCTAAT TTCCTACTGG GGCGAAAAAA TTGGAGAGAA GATCAAAAAG 
ATTGCAAACT GTTTTCACTG TCATATTTAT CCGTATGCTG ATGATGAAAC TTCACGCCTT 
GAGACACTAA GAAATCTTCT GGTTCAGATT CAAGACATGC AAAAAGTGTT GTTACAGACA 
GAAGGTTACC TCTCACAAGT ACTTTCCCGA GCTGCATCAG CTCTGCACCA CTGGAGAGTT 
AGTGTGCGAA AGATGAAACA TATTTATCTC ATTCTGAACT TATGCAGTGT ACGGGAGCGT 
TGCCTGATTG GTGAAGTGTG GTGTCCTGTG GTTGATCTTC CCCTTCTTCA AAGTGCACTT 
ACCCGGGCGT CGGAGAGCAG TGGAGGCGGT GGAGAGTCTT TCTGTCATCG AATACCTTGT 
GCTTTTAGCC CTCCAACTCT TATTCGTACC AATAAATTCA CTTCAGGTTT CCAGGGAATT 
GTTGATGCTT ATGGAGTAGC CACATATCAG GAGGTGAATC CAGCTATCTT CTCAATCATA 
ACGTTTCCAT TCCTGTTTGC TGTGATGTTT GGGGATGTGG GACATGGTGC GATCATGTTC 
CTCTTTGCTC TCTGGCTGGT TCTTGGTGAG AATGATCCAA AACTTAGACG TTCTGAAGAT 
GAGATATTTT CAATGTGTTT CGGGGGGCGT TACCTTATCC TGCTGATGGG AGCACTCTCG 
GTATACACAG GGTTTGTGTA TAATGAGTGT TTCAGTCGAC CAGCTGTCAT TTTTAACTCA 
GGATGGAGTG TTGCTTCTAT GGCAAGAGCC AACAACTGGA CGTCAGACTC AATAAATAAA 
TTGCCTCCAA TTCCTCTGAA CCCCAACATT ACTGGTGTGT TTACTGCACC ATATCCATTT 
GGCATTGATC CTATCTGGAG TCTTGCAGTA AATCGACTTA CTTTCTTGAA CTCCTTCAAG 
ATGAAAATGT CTGTTATTTT GGGTGTTTGT CACATGGCAT TTGGTGTCTG CCTAAGTGTC 
TTTAACTTTG TTCACTTTAG GCAAATATAC CGCATTTTCC TCATAACCCT TCCGGAATTG 
TTGTTTCTAC TTTGTCTCTT TGGGTACCTG GTTTTTATGG TGGTGTATAA ATGGATTGTG 
CTGACAGCTG AGGATGCTGA GTGGGCTCCA AGTATACTTA TACATTTTAT AGACATGTTC 
CTGTTTACTC AGAACCCTGG AAACAGAGAT TTGTACCAGG GGCAGCAAGT TGTGCAGACA 
GTTTTAGTGA TTGTTGCTAT ACTATGCATC CCAGTTCTGT TGCTGGGGGA TCCAATATGT 
CTCCTTATTC AGCATCGGAG TAAAAAAGAC AGCTCTCATC AGAATCGGTG CTCGCTGAAT 
AATGGGGATC GAGAAGCTCT ACTGGAAGAT GAGATTACAG TACCCACAGG ACATGGACAT 
GGCAGTGAAA AGTTTGATAC AGCTGAAGTT TTCATGCATC AAATGATTCA TACTATAGAG 
TACTGTCTGG GCTGCATATC TAATACAGCC TCTTACCTTC GACTGTGGGC GCTCAGCTTG 
GCACATGCAC AGCTGTCTGA GGTCCTGTGG AATATGGTGA TTAGAATTGG ATTCTCCAAG 
TTATCCTTAA CATGGGGCAT TGTTCTTGTT CCAATCTTTG CTTTTTTTGC TGTCCTGACA 
GTCGCCATTT TGCTTATTAT GGAGGGACTG TCTGCTTTTT TACATGCTCT CCGTCTGCAC 
TGGGTGGAGT TCCAAAACAA ATTTTATTCT GGTGAAGGAT ACAAATTCTT TCCCTTTTGC 
TTTGAGACAA TGTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tcirg1 ( XM_031905097.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031760957.1 Xenopus tropicalis T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 (tcirg1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa61459
Clone ID Related Accession (Same CDS sequence)	XM_031905097.1
Accession Version	XM_031905097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	V-type proton ATPase 116 kDa subunit a isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGCC CTCTCTTTGC TTACTTCCCC ATTCCTCCAC CCACACTGTT GATACACAAG 
AAAGAGGAAG TGCCTTTGCT TGTGTTGTTG GAAAGTCGGC AGAAAGGTTT GTGCTTTTTC 
CTGAAAATGG GCTCGCTATT TCGGGGAGAA GAGATGTGCC TAGCACAGTT ATTTCTGCAG 
TCTGGTAGTG AGTATCAATG TGTTAGTGCA CTGGGAGAAC TTGGACTAGT GGAATTCCGG 
GATCTCAATC AAAATGTCAA TTCCTTTCAG CGCCGATATG TATCAGAGAT AAGACGTTGT 
GATGAAATGG AGACAACATT CTCATACCTA GAACGAGAAT TGCGTAAAGC TGGAGTACAA 
GTTCCTGAGT CTGAAATGTC TCCGCCTGCT CCCTTACCGA GAGATGCCAT CAGAATGCAA 
GAAGAATCTG AGCAGCTGGC AAAAGAGCTT AGAGAGGTGT CTCAAAATCG ACAGACTCTA 
CAAGAGCGTT TGAGAGAGCT ACTGGAATAT GCCAATATCC TTCGGGAGAG CCAGAGATTT 
ACTGGGCCAC TGCTGGAGTC TGAAGCACAA TGGAAGGACA GGTCTGAGGA TGATCCACTG 
CTGGACCCTG CTGTAGTCAA TAAACAAGAC CTCAGAGTCA GCTTTATGGC AGGTGTTATT 
CACCCATGGC GCATGAACTC ATTTGAACGT TTGTTGTGGC GGGCTTGCCG TGGTTACCTG 
ATTGTCAACT TTGTAGAAAT GTCAGAGCCA ATGGAAGATT TGGTGACTAT CAAAAAGATT 
GCAAACTGTT TTCACTGTCA TATTTATCCG TATGCTGATG ATGAAACTTC ACGCCTTGAG 
ACACTAAGAA ATCTTCTGGT TCAGATTCAA GACATGCAAA AAGTGTTGTT ACAGACAGAA 
GGTTACCTCT CACAAGTACT TTCCCGAGCT GCATCAGCTC TGCACCACTG GAGAGTTAGT 
GTGCGAAAGA TGAAACATAT TTATCTCATT CTGAACTTAT GCAGTGTACG GGAGCGTTGC 
CTGATTGGTG AAGTGTGGTG TCCTGTGGTT GATCTTCCCC TTCTTCAAAG TGCACTTACC 
CGGGCGTCGG AGAGCAGTGG AGGCGGTGGA GAGTCTTTCT GTCATCGAAT ACCTTGTGCT 
TTTAGCCCTC CAACTCTTAT TCGTACCAAT AAATTCACTT CAGGTTTCCA GGGAATTGTT 
GATGCTTATG GAGTAGCCAC ATATCAGGAG GTGAATCCAG CTATCTTCTC AATCATAACG 
TTTCCATTCC TGTTTGCTGT GATGTTTGGG GATGTGGGAC ATGGTGCGAT CATGTTCCTC 
TTTGCTCTCT GGCTGGTTCT TGGTGAGAAT GATCCAAAAC TTAGACGTTC TGAAGATGAG 
ATATTTTCAA TGTGTTTCGG GGGGCGTTAC CTTATCCTGC TGATGGGAGC ACTCTCGGTA 
TACACAGGGT TTGTGTATAA TGAGTGTTTC AGTCGACCAG CTGTCATTTT TAACTCAGGA 
TGGAGTGTTG CTTCTATGGC AAGAGCCAAC AACTGGACGT CAGACTCAAT AAATAAATTG 
CCTCCAATTC CTCTGAACCC CAACATTACT GGTGTGTTTA CTGCACCATA TCCATTTGGC 
ATTGATCCTA TCTGGAGTCT TGCAGTAAAT CGACTTACTT TCTTGAACTC CTTCAAGATG 
AAAATGTCTG TTATTTTGGG TGTTTGTCAC ATGGCATTTG GTGTCTGCCT AAGTGTCTTT 
AACTTTGTTC ACTTTAGGCA AATATACCGC ATTTTCCTCA TAACCCTTCC GGAATTGTTG 
TTTCTACTTT GTCTCTTTGG GTACCTGGTT TTTATGGTGG TGTATAAATG GATTGTGCTG 
ACAGCTGAGG ATGCTGAGTG GGCTCCAAGT ATACTTATAC ATTTTATAGA CATGTTCCTG 
TTTACTCAGA ACCCTGGAAA CAGAGATTTG TACCAGGGGC AGCAAGTTGT GCAGACAGTT 
TTAGTGATTG TTGCTATACT ATGCATCCCA GTTCTGTTGC TGGGGGATCC AATATGTCTC 
CTTATTCAGC ATCGGAGTAA AAAAGACAGC TCTCATCAGA ATCGGTGCTC GCTGAATAAT 
GGGGATCGAG AAGCTCTACT GGAAGATGAG ATTACAGTAC CCACAGGACA TGGACATGGC 
AGTGAAAAGT TTGATACAGC TGAAGTTTTC ATGCATCAAA TGATTCATAC TATAGAGTAC 
TGTCTGGGCT GCATATCTAA TACAGCCTCT TACCTTCGAC TGTGGGCGCT CAGCTTGGCA 
CATGCACAGC TGTCTGAGGT CCTGTGGAAT ATGGTGATTA GAATTGGATT CTCCAAGTTA 
TCCTTAACAT GGGGCATTGT TCTTGTTCCA ATCTTTGCTT TTTTTGCTGT CCTGACAGTC 
GCCATTTTGC TTATTATGGA GGGACTGTCT GCTTTTTTAC ATGCTCTCCG TCTGCACTGG 
GTGGAGTTCC AAAACAAATT TTATTCTGGT GAAGGATACA AATTCTTTCC CTTTTGCTTT 
GAGACAATGT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031760957.1
CDS	16..2550
Translation	MARPLFAYFPIPPPTLLIHKKEEVPLLVLLESRQKGLCFFLKMGSLFRGEEMCLAQLFLQSGSEYQCVSALGELGLVEFRDLNQNVNSFQRRYVSEIRRCDEMETTFSYLERELRKAGVQVPESEMSPPAPLPRDAIRMQEESEQLAKELREVSQNRQTLQERLRELLEYANILRESQRFTGPLLESEAQWKDRSEDDPLLDPAVVNKQDLRVSFMAGVIHPWRMNSFERLLWRACRGYLIVNFVEMSEPMEDLVTIKKIANCFHCHIYPYADDETSRLETLRNLLVQIQDMQKVLLQTEGYLSQVLSRAASALHHWRVSVRKMKHIYLILNLCSVRERCLIGEVWCPVVDLPLLQSALTRASESSGGGGESFCHRIPCAFSPPTLIRTNKFTSGFQGIVDAYGVATYQEVNPAIFSIITFPFLFAVMFGDVGHGAIMFLFALWLVLGENDPKLRRSEDEIFSMCFGGRYLILLMGALSVYTGFVYNECFSRPAVIFNSGWSVASMARANNWTSDSINKLPPIPLNPNITGVFTAPYPFGIDPIWSLAVNRLTFLNSFKMKMSVILGVCHMAFGVCLSVFNFVHFRQIYRIFLITLPELLFLLCLFGYLVFMVVYKWIVLTAEDAEWAPSILIHFIDMFLFTQNPGNRDLYQGQQVVQTVLVIVAILCIPVLLLGDPICLLIQHRSKKDSSHQNRCSLNNGDREALLEDEITVPTGHGHGSEKFDTAEVFMHQMIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWNMVIRIGFSKLSLTWGIVLVPIFAFFAVLTVAILLIMEGLSAFLHALRLHWVEFQNKFYSGEGYKFFPFCFETMY

Target ORF information:

RefSeq Version	XM_031905097.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 (tcirg1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905097.1

ORF Insert Sequence:

ATGGCCCGCC CTCTCTTTGC TTACTTCCCC ATTCCTCCAC CCACACTGTT GATACACAAG 
AAAGAGGAAG TGCCTTTGCT TGTGTTGTTG GAAAGTCGGC AGAAAGGTTT GTGCTTTTTC 
CTGAAAATGG GCTCGCTATT TCGGGGAGAA GAGATGTGCC TAGCACAGTT ATTTCTGCAG 
TCTGGTAGTG AGTATCAATG TGTTAGTGCA CTGGGAGAAC TTGGACTAGT GGAATTCCGG 
GATCTCAATC AAAATGTCAA TTCCTTTCAG CGCCGATATG TATCAGAGAT AAGACGTTGT 
GATGAAATGG AGACAACATT CTCATACCTA GAACGAGAAT TGCGTAAAGC TGGAGTACAA 
GTTCCTGAGT CTGAAATGTC TCCGCCTGCT CCCTTACCGA GAGATGCCAT CAGAATGCAA 
GAAGAATCTG AGCAGCTGGC AAAAGAGCTT AGAGAGGTGT CTCAAAATCG ACAGACTCTA 
CAAGAGCGTT TGAGAGAGCT ACTGGAATAT GCCAATATCC TTCGGGAGAG CCAGAGATTT 
ACTGGGCCAC TGCTGGAGTC TGAAGCACAA TGGAAGGACA GGTCTGAGGA TGATCCACTG 
CTGGACCCTG CTGTAGTCAA TAAACAAGAC CTCAGAGTCA GCTTTATGGC AGGTGTTATT 
CACCCATGGC GCATGAACTC ATTTGAACGT TTGTTGTGGC GGGCTTGCCG TGGTTACCTG 
ATTGTCAACT TTGTAGAAAT GTCAGAGCCA ATGGAAGATT TGGTGACTAT CAAAAAGATT 
GCAAACTGTT TTCACTGTCA TATTTATCCG TATGCTGATG ATGAAACTTC ACGCCTTGAG 
ACACTAAGAA ATCTTCTGGT TCAGATTCAA GACATGCAAA AAGTGTTGTT ACAGACAGAA 
GGTTACCTCT CACAAGTACT TTCCCGAGCT GCATCAGCTC TGCACCACTG GAGAGTTAGT 
GTGCGAAAGA TGAAACATAT TTATCTCATT CTGAACTTAT GCAGTGTACG GGAGCGTTGC 
CTGATTGGTG AAGTGTGGTG TCCTGTGGTT GATCTTCCCC TTCTTCAAAG TGCACTTACC 
CGGGCGTCGG AGAGCAGTGG AGGCGGTGGA GAGTCTTTCT GTCATCGAAT ACCTTGTGCT 
TTTAGCCCTC CAACTCTTAT TCGTACCAAT AAATTCACTT CAGGTTTCCA GGGAATTGTT 
GATGCTTATG GAGTAGCCAC ATATCAGGAG GTGAATCCAG CTATCTTCTC AATCATAACG 
TTTCCATTCC TGTTTGCTGT GATGTTTGGG GATGTGGGAC ATGGTGCGAT CATGTTCCTC 
TTTGCTCTCT GGCTGGTTCT TGGTGAGAAT GATCCAAAAC TTAGACGTTC TGAAGATGAG 
ATATTTTCAA TGTGTTTCGG GGGGCGTTAC CTTATCCTGC TGATGGGAGC ACTCTCGGTA 
TACACAGGGT TTGTGTATAA TGAGTGTTTC AGTCGACCAG CTGTCATTTT TAACTCAGGA 
TGGAGTGTTG CTTCTATGGC AAGAGCCAAC AACTGGACGT CAGACTCAAT AAATAAATTG 
CCTCCAATTC CTCTGAACCC CAACATTACT GGTGTGTTTA CTGCACCATA TCCATTTGGC 
ATTGATCCTA TCTGGAGTCT TGCAGTAAAT CGACTTACTT TCTTGAACTC CTTCAAGATG 
AAAATGTCTG TTATTTTGGG TGTTTGTCAC ATGGCATTTG GTGTCTGCCT AAGTGTCTTT 
AACTTTGTTC ACTTTAGGCA AATATACCGC ATTTTCCTCA TAACCCTTCC GGAATTGTTG 
TTTCTACTTT GTCTCTTTGG GTACCTGGTT TTTATGGTGG TGTATAAATG GATTGTGCTG 
ACAGCTGAGG ATGCTGAGTG GGCTCCAAGT ATACTTATAC ATTTTATAGA CATGTTCCTG 
TTTACTCAGA ACCCTGGAAA CAGAGATTTG TACCAGGGGC AGCAAGTTGT GCAGACAGTT 
TTAGTGATTG TTGCTATACT ATGCATCCCA GTTCTGTTGC TGGGGGATCC AATATGTCTC 
CTTATTCAGC ATCGGAGTAA AAAAGACAGC TCTCATCAGA ATCGGTGCTC GCTGAATAAT 
GGGGATCGAG AAGCTCTACT GGAAGATGAG ATTACAGTAC CCACAGGACA TGGACATGGC 
AGTGAAAAGT TTGATACAGC TGAAGTTTTC ATGCATCAAA TGATTCATAC TATAGAGTAC 
TGTCTGGGCT GCATATCTAA TACAGCCTCT TACCTTCGAC TGTGGGCGCT CAGCTTGGCA 
CATGCACAGC TGTCTGAGGT CCTGTGGAAT ATGGTGATTA GAATTGGATT CTCCAAGTTA 
TCCTTAACAT GGGGCATTGT TCTTGTTCCA ATCTTTGCTT TTTTTGCTGT CCTGACAGTC 
GCCATTTTGC TTATTATGGA GGGACTGTCT GCTTTTTTAC ATGCTCTCCG TCTGCACTGG 
GTGGAGTTCC AAAACAAATT TTATTCTGGT GAAGGATACA AATTCTTTCC CTTTTGCTTT 
GAGACAATGT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tcirg1 ( XM_031905098.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031760958.1 Xenopus tropicalis T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 (tcirg1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa61460
Clone ID Related Accession (Same CDS sequence)	XM_031905098.1
Accession Version	XM_031905098.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	V-type proton ATPase 116 kDa subunit a isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGCC CTCTCTTTGC TTACTTCCCC ATTCCTCCAC CCACACTGTT GATACACAAG 
AAAGAGGAAG TGCCTTTGCT TGTGTTGTTG GAAAGTCGGC AGAAAGGTTT GTGCTTTTTC 
CTGAAAATGG GCTCGCTATT TCGGGGAGAA GAGATGTGCC TAGCACAGTT ATTTCTGCAG 
TCTGGTAGTG AGTATCAATG TGTTAGTGCA CTGGGAGAAC TTGGACTAGT GGAATTCCGG 
GATCTCAATC AAAATGTCAA TTCCTTTCAG CGCCGATATG TATCAGAGAT AAGACGTTGT 
GATGAAATGG AGACAACATT CTCATACCTA GAACGAGAAT TGCGTAAAGC TGGAGTACAA 
GTTCCTGAGT CTGAAATGTC TCCGCCTGCT CCCTTACCGA GAGATGCCAT CAGAATGCAA 
GAAGAATCTG AGCAGCTGGC AAAAGAGCTT AGAGAGGTGT CTCAAAATCG ACAGACTCTA 
CAAGAGCGTT TGAGAGAGCT ACTGGAATAT GCCAATATCC TTCGGGAGAG CCAGAGATTT 
ACTGGGCCAC TGCTGGAGTC TGAAGCACAA TGGAAGGACA GGTCTGAGGA TGATCCACTG 
CTGGACCCTG CTGTAGTCAA TAAACAAGAC CTCAGAGTCA GCTTTATGGC AGGTGTTATT 
CACCCATGGC GCATGAACTC ATTTGAACGT TTGTTGTGGC GGGCTTGCCG TGGTTACCTG 
ATTGTCAACT TTGTAGAAAT GTCAGAGCCA ATGGAAGATT TGGTGACTGT GTTGTTACAG 
ACAGAAGGTT ACCTCTCACA AGTACTTTCC CGAGCTGCAT CAGCTCTGCA CCACTGGAGA 
GTTAGTGTGC GAAAGATGAA ACATATTTAT CTCATTCTGA ACTTATGCAG TGTACGGGAG 
CGTTGCCTGA TTGGTGAAGT GTGGTGTCCT GTGGTTGATC TTCCCCTTCT TCAAAGTGCA 
CTTACCCGGG CGTCGGAGAG CAGTGGAGGC GGTGGAGAGT CTTTCTGTCA TCGAATACCT 
TGTGCTTTTA GCCCTCCAAC TCTTATTCGT ACCAATAAAT TCACTTCAGG TTTCCAGGGA 
ATTGTTGATG CTTATGGAGT AGCCACATAT CAGGAGGTGA ATCCAGCTAT CTTCTCAATC 
ATAACGTTTC CATTCCTGTT TGCTGTGATG TTTGGGGATG TGGGACATGG TGCGATCATG 
TTCCTCTTTG CTCTCTGGCT GGTTCTTGGT GAGAATGATC CAAAACTTAG ACGTTCTGAA 
GATGAGATAT TTTCAATGTG TTTCGGGGGG CGTTACCTTA TCCTGCTGAT GGGAGCACTC 
TCGGTATACA CAGGGTTTGT GTATAATGAG TGTTTCAGTC GACCAGCTGT CATTTTTAAC 
TCAGGATGGA GTGTTGCTTC TATGGCAAGA GCCAACAACT GGACGTCAGA CTCAATAAAT 
AAATTGCCTC CAATTCCTCT GAACCCCAAC ATTACTGGTG TGTTTACTGC ACCATATCCA 
TTTGGCATTG ATCCTATCTG GAGTCTTGCA GTAAATCGAC TTACTTTCTT GAACTCCTTC 
AAGATGAAAA TGTCTGTTAT TTTGGGTGTT TGTCACATGG CATTTGGTGT CTGCCTAAGT 
GTCTTTAACT TTGTTCACTT TAGGCAAATA TACCGCATTT TCCTCATAAC CCTTCCGGAA 
TTGTTGTTTC TACTTTGTCT CTTTGGGTAC CTGGTTTTTA TGGTGGTGTA TAAATGGATT 
GTGCTGACAG CTGAGGATGC TGAGTGGGCT CCAAGTATAC TTATACATTT TATAGACATG 
TTCCTGTTTA CTCAGAACCC TGGAAACAGA GATTTGTACC AGGGGCAGCA AGTTGTGCAG 
ACAGTTTTAG TGATTGTTGC TATACTATGC ATCCCAGTTC TGTTGCTGGG GGATCCAATA 
TGTCTCCTTA TTCAGCATCG GAGTAAAAAA GACAGCTCTC ATCAGAATCG GTGCTCGCTG 
AATAATGGGG ATCGAGAAGC TCTACTGGAA GATGAGATTA CAGTACCCAC AGGACATGGA 
CATGGCAGTG AAAAGTTTGA TACAGCTGAA GTTTTCATGC ATCAAATGAT TCATACTATA 
GAGTACTGTC TGGGCTGCAT ATCTAATACA GCCTCTTACC TTCGACTGTG GGCGCTCAGC 
TTGGCACATG CACAGCTGTC TGAGGTCCTG TGGAATATGG TGATTAGAAT TGGATTCTCC 
AAGTTATCCT TAACATGGGG CATTGTTCTT GTTCCAATCT TTGCTTTTTT TGCTGTCCTG 
ACAGTCGCCA TTTTGCTTAT TATGGAGGGA CTGTCTGCTT TTTTACATGC TCTCCGTCTG 
CACTGGGTGG AGTTCCAAAA CAAATTTTAT TCTGGTGAAG GATACAAATT CTTTCCCTTT 
TGCTTTGAGA CAATGTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031760958.1
CDS	16..2436
Translation	MARPLFAYFPIPPPTLLIHKKEEVPLLVLLESRQKGLCFFLKMGSLFRGEEMCLAQLFLQSGSEYQCVSALGELGLVEFRDLNQNVNSFQRRYVSEIRRCDEMETTFSYLERELRKAGVQVPESEMSPPAPLPRDAIRMQEESEQLAKELREVSQNRQTLQERLRELLEYANILRESQRFTGPLLESEAQWKDRSEDDPLLDPAVVNKQDLRVSFMAGVIHPWRMNSFERLLWRACRGYLIVNFVEMSEPMEDLVTVLLQTEGYLSQVLSRAASALHHWRVSVRKMKHIYLILNLCSVRERCLIGEVWCPVVDLPLLQSALTRASESSGGGGESFCHRIPCAFSPPTLIRTNKFTSGFQGIVDAYGVATYQEVNPAIFSIITFPFLFAVMFGDVGHGAIMFLFALWLVLGENDPKLRRSEDEIFSMCFGGRYLILLMGALSVYTGFVYNECFSRPAVIFNSGWSVASMARANNWTSDSINKLPPIPLNPNITGVFTAPYPFGIDPIWSLAVNRLTFLNSFKMKMSVILGVCHMAFGVCLSVFNFVHFRQIYRIFLITLPELLFLLCLFGYLVFMVVYKWIVLTAEDAEWAPSILIHFIDMFLFTQNPGNRDLYQGQQVVQTVLVIVAILCIPVLLLGDPICLLIQHRSKKDSSHQNRCSLNNGDREALLEDEITVPTGHGHGSEKFDTAEVFMHQMIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWNMVIRIGFSKLSLTWGIVLVPIFAFFAVLTVAILLIMEGLSAFLHALRLHWVEFQNKFYSGEGYKFFPFCFETMY

Target ORF information:

RefSeq Version	XM_031905098.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 (tcirg1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905098.1

ORF Insert Sequence:

ATGGCCCGCC CTCTCTTTGC TTACTTCCCC ATTCCTCCAC CCACACTGTT GATACACAAG 
AAAGAGGAAG TGCCTTTGCT TGTGTTGTTG GAAAGTCGGC AGAAAGGTTT GTGCTTTTTC 
CTGAAAATGG GCTCGCTATT TCGGGGAGAA GAGATGTGCC TAGCACAGTT ATTTCTGCAG 
TCTGGTAGTG AGTATCAATG TGTTAGTGCA CTGGGAGAAC TTGGACTAGT GGAATTCCGG 
GATCTCAATC AAAATGTCAA TTCCTTTCAG CGCCGATATG TATCAGAGAT AAGACGTTGT 
GATGAAATGG AGACAACATT CTCATACCTA GAACGAGAAT TGCGTAAAGC TGGAGTACAA 
GTTCCTGAGT CTGAAATGTC TCCGCCTGCT CCCTTACCGA GAGATGCCAT CAGAATGCAA 
GAAGAATCTG AGCAGCTGGC AAAAGAGCTT AGAGAGGTGT CTCAAAATCG ACAGACTCTA 
CAAGAGCGTT TGAGAGAGCT ACTGGAATAT GCCAATATCC TTCGGGAGAG CCAGAGATTT 
ACTGGGCCAC TGCTGGAGTC TGAAGCACAA TGGAAGGACA GGTCTGAGGA TGATCCACTG 
CTGGACCCTG CTGTAGTCAA TAAACAAGAC CTCAGAGTCA GCTTTATGGC AGGTGTTATT 
CACCCATGGC GCATGAACTC ATTTGAACGT TTGTTGTGGC GGGCTTGCCG TGGTTACCTG 
ATTGTCAACT TTGTAGAAAT GTCAGAGCCA ATGGAAGATT TGGTGACTGT GTTGTTACAG 
ACAGAAGGTT ACCTCTCACA AGTACTTTCC CGAGCTGCAT CAGCTCTGCA CCACTGGAGA 
GTTAGTGTGC GAAAGATGAA ACATATTTAT CTCATTCTGA ACTTATGCAG TGTACGGGAG 
CGTTGCCTGA TTGGTGAAGT GTGGTGTCCT GTGGTTGATC TTCCCCTTCT TCAAAGTGCA 
CTTACCCGGG CGTCGGAGAG CAGTGGAGGC GGTGGAGAGT CTTTCTGTCA TCGAATACCT 
TGTGCTTTTA GCCCTCCAAC TCTTATTCGT ACCAATAAAT TCACTTCAGG TTTCCAGGGA 
ATTGTTGATG CTTATGGAGT AGCCACATAT CAGGAGGTGA ATCCAGCTAT CTTCTCAATC 
ATAACGTTTC CATTCCTGTT TGCTGTGATG TTTGGGGATG TGGGACATGG TGCGATCATG 
TTCCTCTTTG CTCTCTGGCT GGTTCTTGGT GAGAATGATC CAAAACTTAG ACGTTCTGAA 
GATGAGATAT TTTCAATGTG TTTCGGGGGG CGTTACCTTA TCCTGCTGAT GGGAGCACTC 
TCGGTATACA CAGGGTTTGT GTATAATGAG TGTTTCAGTC GACCAGCTGT CATTTTTAAC 
TCAGGATGGA GTGTTGCTTC TATGGCAAGA GCCAACAACT GGACGTCAGA CTCAATAAAT 
AAATTGCCTC CAATTCCTCT GAACCCCAAC ATTACTGGTG TGTTTACTGC ACCATATCCA 
TTTGGCATTG ATCCTATCTG GAGTCTTGCA GTAAATCGAC TTACTTTCTT GAACTCCTTC 
AAGATGAAAA TGTCTGTTAT TTTGGGTGTT TGTCACATGG CATTTGGTGT CTGCCTAAGT 
GTCTTTAACT TTGTTCACTT TAGGCAAATA TACCGCATTT TCCTCATAAC CCTTCCGGAA 
TTGTTGTTTC TACTTTGTCT CTTTGGGTAC CTGGTTTTTA TGGTGGTGTA TAAATGGATT 
GTGCTGACAG CTGAGGATGC TGAGTGGGCT CCAAGTATAC TTATACATTT TATAGACATG 
TTCCTGTTTA CTCAGAACCC TGGAAACAGA GATTTGTACC AGGGGCAGCA AGTTGTGCAG 
ACAGTTTTAG TGATTGTTGC TATACTATGC ATCCCAGTTC TGTTGCTGGG GGATCCAATA 
TGTCTCCTTA TTCAGCATCG GAGTAAAAAA GACAGCTCTC ATCAGAATCG GTGCTCGCTG 
AATAATGGGG ATCGAGAAGC TCTACTGGAA GATGAGATTA CAGTACCCAC AGGACATGGA 
CATGGCAGTG AAAAGTTTGA TACAGCTGAA GTTTTCATGC ATCAAATGAT TCATACTATA 
GAGTACTGTC TGGGCTGCAT ATCTAATACA GCCTCTTACC TTCGACTGTG GGCGCTCAGC 
TTGGCACATG CACAGCTGTC TGAGGTCCTG TGGAATATGG TGATTAGAAT TGGATTCTCC 
AAGTTATCCT TAACATGGGG CATTGTTCTT GTTCCAATCT TTGCTTTTTT TGCTGTCCTG 
ACAGTCGCCA TTTTGCTTAT TATGGAGGGA CTGTCTGCTT TTTTACATGC TCTCCGTCTG 
CACTGGGTGG AGTTCCAAAA CAAATTTTAT TCTGGTGAAG GATACAAATT CTTTCCCTTT 
TGCTTTGAGA CAATGTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tcirg1 ( XM_031905099.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031760959.1 Xenopus tropicalis T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 (tcirg1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa61461
Clone ID Related Accession (Same CDS sequence)	XM_031905099.1
Accession Version	XM_031905099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	V-type proton ATPase 116 kDa subunit a isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGCC CTCTCTTTGC TTACTTCCCC ATTCCTCCAC CCACACTGTT GATACACAAG 
AAAGAGGAAG TGCCTTTGCT TGTGTTGTTG GAAAGTCGGC AGAAAGGTTT GTGCTTTTTC 
CTGAAAATGG GCTCGCTATT TCGGGGAGAA GAGATGTGCC TAGCACAGTT ATTTCTGCAG 
TCTGGTAGTG AGTATCAATG TGTTAGTGCA CTGGGAGAAC TTGGACTAGT GGAATTCCGG 
GATCTCAATC AAAATGTCAA TTCCTTTCAG CGCCGATATG TATCAGAGAT AAGACGTTGT 
GATGAAATGG AGACAACATT CTCATACCTA GAACGAGAAT TGCGTAAAGC TGGAGTACAA 
GTTCCTGAGT CTGAAATGTC TCCGCCTGCT CCCTTACCGA GAGATGCCAT CAGAATGCAA 
GAAGAATCTG AGCAGCTGGC AAAAGAGCTT AGAGAGGTGT CTCAAAATCG ACAGACTCTA 
CAAGAGCGTT TGAGAGAGCT ACTGGAATAT GCCAATATCC TTCGGGAGAG CCAGAGATTT 
ACTGGGCCAC TGCTGGAGTC TGAAGCACAA TGGAAGGACA GGTCTGAGGA TGATCCACTG 
CTGGACCCTG CTGTAGTCAA TAAACAAGAC CTCAGAGTCA GCTTTATGGC AGGTGTTATT 
CACCCATGGC GCATGAACTC ATTTGAACGT TTGTTGTGGC GGGCTTGCCG TGGTTACCTG 
ATTGTCAACT TTGTAGAAAT GTCAGAGCCA ATGGAAGATT TGGTGACTGG TGAGAGTGTC 
ACTCAAATTA TATTTCTAAT TTCCTACTGG GGCGAAAAAA TTGGAGAGAA GATCAAAAAG 
ATTGCAAACT GTTTTCACTG TCATATTTAT CCGTATGCTG ATGATGAAAC TTCACGCCTT 
GAGACACTAA GAAATCTTCT GGTTCAGATT CAAGACATGC AAAAAGTGTT GTTACAGACA 
GAAGGTTACC TCTCACAAGT ACTTTCCCGA GCTGCATCAG CTCTGCACCA CTGGAGAGTT 
AGTGTGCGAA AGATGAAACA TATTTATCTC ATTCTGAACT TATGCAGTGT ACGGGAGCGT 
TGCCTGATTG GTGAAGTGTG GTGTCCTGTG GTTGATCTTC CCCTTCTTCA AAGTGCACTT 
ACCCGGGCGT CGGAGAGCAG TGGAGGCGGT GGAGAGTCTT TCTGTCATCG AATACCTTGT 
GCTTTTAGCC CTCCAACTCT TATTCGTACC AATAAATTCA CTTCAGGTTT CCAGGGAATT 
GTTGATGCTT ATGGAGTAGC CACATATCAG GAGGTGAATC CAGCTATCTT CTCAATCATA 
ACGTTTCCAT TCCTGTTTGC TGTGATGTTT GGGGATGTGG GACATGGTGC GATCATGTTC 
CTCTTTGCTC TCTGGCTGGT TCTTGGTGAG AATGATCCAA AACTTAGACG TTCTGAAGAT 
GAGATATTTT CAATGTGTTT CGGGGGGCGT TACCTTATCC TGCTGATGGG AGCACTCTCG 
GTATACACAG GGTTTGTGTA TAATGAGTGT TTCAGTCGAC CAGCTGTCAT TTTTAACTCA 
GGATGGAGTG TTGCTTCTAT GGCAAGAGCC AACAACTGGA CGTCAGACTC AATAAATAAA 
TTGCCTCCAA TTCCTCTGAA CCCCAACATT ACTGGTGTGT TTACTGCACC ATATCCATTT 
GGCATTGATC CTATCTGGAG TCTTGCAGTA AATCGACTTA CTTTCTTGAA CTCCTTCAAG 
ATGAAAATGT CTGTTATTTT GGGTGTTTGT CACATGGCAT TTGGTGTCTG CCTAAGTGTC 
TTTAACTTTG TTCACTTTAG GCAAATATAC CGCATTTTCC TCATAACCCT TCCGGAATTG 
TTGTTTCTAC TTTGTCTCTT TGGGTACCTG GTTTTTATGG TGGTGTATAA ATGGATTGTG 
CTGACAGCTG AGGATGCTGA GTGGGCTCCA AGTATACTTA TACATTTTAT AGACATGTTC 
CTGTTTACTC AGAACCCTGG AAACAGAGAT TTGTACCAGG GGCAGCAAGT TGTGCAGACA 
GTTTTAGTGA TTGTTGCTAT ACTATGCATC CCAGTTCTGT TGCTGGGGGA TCCAATATGT 
CTCCTTATTC AGCATCGGAG TAAAAAAGAC AGCTCTCATC AGAATCGGTG CTCGCTGAAT 
AATGGGGATC GAGAAGCTCT ACTGGAAGAT GAGATTACAG TACCCACAGG ACATGGACAT 
GGCAGTGAAA AGTTTGATAC AGCTGAAGTT TTCATGCATC AAATGATTCA TACTATAGAG 
TACTGTCTGG GCTGCATATC TAATACAGCC TCTTACCTTC GACTGTGGGC GCTCAGCTTG 
GCACATAGCT GTCTGAGGTC CTGTGGAATA TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031760959.1
CDS	16..2391
Translation	MARPLFAYFPIPPPTLLIHKKEEVPLLVLLESRQKGLCFFLKMGSLFRGEEMCLAQLFLQSGSEYQCVSALGELGLVEFRDLNQNVNSFQRRYVSEIRRCDEMETTFSYLERELRKAGVQVPESEMSPPAPLPRDAIRMQEESEQLAKELREVSQNRQTLQERLRELLEYANILRESQRFTGPLLESEAQWKDRSEDDPLLDPAVVNKQDLRVSFMAGVIHPWRMNSFERLLWRACRGYLIVNFVEMSEPMEDLVTGESVTQIIFLISYWGEKIGEKIKKIANCFHCHIYPYADDETSRLETLRNLLVQIQDMQKVLLQTEGYLSQVLSRAASALHHWRVSVRKMKHIYLILNLCSVRERCLIGEVWCPVVDLPLLQSALTRASESSGGGGESFCHRIPCAFSPPTLIRTNKFTSGFQGIVDAYGVATYQEVNPAIFSIITFPFLFAVMFGDVGHGAIMFLFALWLVLGENDPKLRRSEDEIFSMCFGGRYLILLMGALSVYTGFVYNECFSRPAVIFNSGWSVASMARANNWTSDSINKLPPIPLNPNITGVFTAPYPFGIDPIWSLAVNRLTFLNSFKMKMSVILGVCHMAFGVCLSVFNFVHFRQIYRIFLITLPELLFLLCLFGYLVFMVVYKWIVLTAEDAEWAPSILIHFIDMFLFTQNPGNRDLYQGQQVVQTVLVIVAILCIPVLLLGDPICLLIQHRSKKDSSHQNRCSLNNGDREALLEDEITVPTGHGHGSEKFDTAEVFMHQMIHTIEYCLGCISNTASYLRLWALSLAHSCLRSCGIW

Target ORF information:

RefSeq Version	XM_031905099.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 (tcirg1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905099.1

ORF Insert Sequence:

ATGGCCCGCC CTCTCTTTGC TTACTTCCCC ATTCCTCCAC CCACACTGTT GATACACAAG 
AAAGAGGAAG TGCCTTTGCT TGTGTTGTTG GAAAGTCGGC AGAAAGGTTT GTGCTTTTTC 
CTGAAAATGG GCTCGCTATT TCGGGGAGAA GAGATGTGCC TAGCACAGTT ATTTCTGCAG 
TCTGGTAGTG AGTATCAATG TGTTAGTGCA CTGGGAGAAC TTGGACTAGT GGAATTCCGG 
GATCTCAATC AAAATGTCAA TTCCTTTCAG CGCCGATATG TATCAGAGAT AAGACGTTGT 
GATGAAATGG AGACAACATT CTCATACCTA GAACGAGAAT TGCGTAAAGC TGGAGTACAA 
GTTCCTGAGT CTGAAATGTC TCCGCCTGCT CCCTTACCGA GAGATGCCAT CAGAATGCAA 
GAAGAATCTG AGCAGCTGGC AAAAGAGCTT AGAGAGGTGT CTCAAAATCG ACAGACTCTA 
CAAGAGCGTT TGAGAGAGCT ACTGGAATAT GCCAATATCC TTCGGGAGAG CCAGAGATTT 
ACTGGGCCAC TGCTGGAGTC TGAAGCACAA TGGAAGGACA GGTCTGAGGA TGATCCACTG 
CTGGACCCTG CTGTAGTCAA TAAACAAGAC CTCAGAGTCA GCTTTATGGC AGGTGTTATT 
CACCCATGGC GCATGAACTC ATTTGAACGT TTGTTGTGGC GGGCTTGCCG TGGTTACCTG 
ATTGTCAACT TTGTAGAAAT GTCAGAGCCA ATGGAAGATT TGGTGACTGG TGAGAGTGTC 
ACTCAAATTA TATTTCTAAT TTCCTACTGG GGCGAAAAAA TTGGAGAGAA GATCAAAAAG 
ATTGCAAACT GTTTTCACTG TCATATTTAT CCGTATGCTG ATGATGAAAC TTCACGCCTT 
GAGACACTAA GAAATCTTCT GGTTCAGATT CAAGACATGC AAAAAGTGTT GTTACAGACA 
GAAGGTTACC TCTCACAAGT ACTTTCCCGA GCTGCATCAG CTCTGCACCA CTGGAGAGTT 
AGTGTGCGAA AGATGAAACA TATTTATCTC ATTCTGAACT TATGCAGTGT ACGGGAGCGT 
TGCCTGATTG GTGAAGTGTG GTGTCCTGTG GTTGATCTTC CCCTTCTTCA AAGTGCACTT 
ACCCGGGCGT CGGAGAGCAG TGGAGGCGGT GGAGAGTCTT TCTGTCATCG AATACCTTGT 
GCTTTTAGCC CTCCAACTCT TATTCGTACC AATAAATTCA CTTCAGGTTT CCAGGGAATT 
GTTGATGCTT ATGGAGTAGC CACATATCAG GAGGTGAATC CAGCTATCTT CTCAATCATA 
ACGTTTCCAT TCCTGTTTGC TGTGATGTTT GGGGATGTGG GACATGGTGC GATCATGTTC 
CTCTTTGCTC TCTGGCTGGT TCTTGGTGAG AATGATCCAA AACTTAGACG TTCTGAAGAT 
GAGATATTTT CAATGTGTTT CGGGGGGCGT TACCTTATCC TGCTGATGGG AGCACTCTCG 
GTATACACAG GGTTTGTGTA TAATGAGTGT TTCAGTCGAC CAGCTGTCAT TTTTAACTCA 
GGATGGAGTG TTGCTTCTAT GGCAAGAGCC AACAACTGGA CGTCAGACTC AATAAATAAA 
TTGCCTCCAA TTCCTCTGAA CCCCAACATT ACTGGTGTGT TTACTGCACC ATATCCATTT 
GGCATTGATC CTATCTGGAG TCTTGCAGTA AATCGACTTA CTTTCTTGAA CTCCTTCAAG 
ATGAAAATGT CTGTTATTTT GGGTGTTTGT CACATGGCAT TTGGTGTCTG CCTAAGTGTC 
TTTAACTTTG TTCACTTTAG GCAAATATAC CGCATTTTCC TCATAACCCT TCCGGAATTG 
TTGTTTCTAC TTTGTCTCTT TGGGTACCTG GTTTTTATGG TGGTGTATAA ATGGATTGTG 
CTGACAGCTG AGGATGCTGA GTGGGCTCCA AGTATACTTA TACATTTTAT AGACATGTTC 
CTGTTTACTC AGAACCCTGG AAACAGAGAT TTGTACCAGG GGCAGCAAGT TGTGCAGACA 
GTTTTAGTGA TTGTTGCTAT ACTATGCATC CCAGTTCTGT TGCTGGGGGA TCCAATATGT 
CTCCTTATTC AGCATCGGAG TAAAAAAGAC AGCTCTCATC AGAATCGGTG CTCGCTGAAT 
AATGGGGATC GAGAAGCTCT ACTGGAAGAT GAGATTACAG TACCCACAGG ACATGGACAT 
GGCAGTGAAA AGTTTGATAC AGCTGAAGTT TTCATGCATC AAATGATTCA TACTATAGAG 
TACTGTCTGG GCTGCATATC TAATACAGCC TCTTACCTTC GACTGTGGGC GCTCAGCTTG 
GCACATAGCT GTCTGAGGTC CTGTGGAATA TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tcirg1 ( XM_031905100.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031760960.1 Xenopus tropicalis T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 (tcirg1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa61462
Clone ID Related Accession (Same CDS sequence)	XM_031905100.1
Accession Version	XM_031905100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	V-type proton ATPase 116 kDa subunit a isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCCGC CTGCTCCCTT ACCGAGAGAT GCCATCAGAA TGCAAGAAGA ATCTGAGCAG 
CTGGCAAAAG AGCTTAGAGA GGTGTCTCAA AATCGACAGA CTCTACAAGA GCGTTTGAGA 
GAGCTACTGG AATATGCCAA TATCCTTCGG GAGAGCCAGA GATTTACTGG GCCACTGCTG 
GAGTCTGAAG CACAATGGAA GGACAGGTCT GAGGATGATC CACTGCTGGA CCCTGCTGTA 
GTCAATAAAC AAGACCTCAG AGTCAGCTTT ATGGCAGGTG TTATTCACCC ATGGCGCATG 
AACTCATTTG AACGTTTGTT GTGGCGGGCT TGCCGTGGTT ACCTGATTGT CAACTTTGTA 
GAAATGTCAG AGCCAATGGA AGATTTGGTG ACTGGTGAGA GTGTCACTCA AATTATATTT 
CTAATTTCCT ACTGGGGCGA AAAAATTGGA GAGAAGATCA AAAAGATTGC AAACTGTTTT 
CACTGTCATA TTTATCCGTA TGCTGATGAT GAAACTTCAC GCCTTGAGAC ACTAAGAAAT 
CTTCTGGTTC AGATTCAAGA CATGCAAAAA GTGTTGTTAC AGACAGAAGG TTACCTCTCA 
CAAGTACTTT CCCGAGCTGC ATCAGCTCTG CACCACTGGA GAGTTAGTGT GCGAAAGATG 
AAACATATTT ATCTCATTCT GAACTTATGC AGTGTACGGG AGCGTTGCCT GATTGGTGAA 
GTGTGGTGTC CTGTGGTTGA TCTTCCCCTT CTTCAAAGTG CACTTACCCG GGCGTCGGAG 
AGCAGTGGAG GCGGTGGAGA GTCTTTCTGT CATCGAATAC CTTGTGCTTT TAGCCCTCCA 
ACTCTTATTC GTACCAATAA ATTCACTTCA GGTTTCCAGG GAATTGTTGA TGCTTATGGA 
GTAGCCACAT ATCAGGAGGT GAATCCAGCT ATCTTCTCAA TCATAACGTT TCCATTCCTG 
TTTGCTGTGA TGTTTGGGGA TGTGGGACAT GGTGCGATCA TGTTCCTCTT TGCTCTCTGG 
CTGGTTCTTG GTGAGAATGA TCCAAAACTT AGACGTTCTG AAGATGAGAT ATTTTCAATG 
TGTTTCGGGG GGCGTTACCT TATCCTGCTG ATGGGAGCAC TCTCGGTATA CACAGGGTTT 
GTGTATAATG AGTGTTTCAG TCGACCAGCT GTCATTTTTA ACTCAGGATG GAGTGTTGCT 
TCTATGGCAA GAGCCAACAA CTGGACGTCA GACTCAATAA ATAAATTGCC TCCAATTCCT 
CTGAACCCCA ACATTACTGG TGTGTTTACT GCACCATATC CATTTGGCAT TGATCCTATC 
TGGAGTCTTG CAGTAAATCG ACTTACTTTC TTGAACTCCT TCAAGATGAA AATGTCTGTT 
ATTTTGGGTG TTTGTCACAT GGCATTTGGT GTCTGCCTAA GTGTCTTTAA CTTTGTTCAC 
TTTAGGCAAA TATACCGCAT TTTCCTCATA ACCCTTCCGG AATTGTTGTT TCTACTTTGT 
CTCTTTGGGT ACCTGGTTTT TATGGTGGTG TATAAATGGA TTGTGCTGAC AGCTGAGGAT 
GCTGAGTGGG CTCCAAGTAT ACTTATACAT TTTATAGACA TGTTCCTGTT TACTCAGAAC 
CCTGGAAACA GAGATTTGTA CCAGGGGCAG CAAGTTGTGC AGACAGTTTT AGTGATTGTT 
GCTATACTAT GCATCCCAGT TCTGTTGCTG GGGGATCCAA TATGTCTCCT TATTCAGCAT 
CGGAGTAAAA AAGACAGCTC TCATCAGAAT CGGTGCTCGC TGAATAATGG GGATCGAGAA 
GCTCTACTGG AAGATGAGAT TACAGTACCC ACAGGACATG GACATGGCAG TGAAAAGTTT 
GATACAGCTG AAGTTTTCAT GCATCAAATG ATTCATACTA TAGAGTACTG TCTGGGCTGC 
ATATCTAATA CAGCCTCTTA CCTTCGACTG TGGGCGCTCA GCTTGGCACA TGCACAGCTG 
TCTGAGGTCC TGTGGAATAT GGTGATTAGA ATTGGATTCT CCAAGTTATC CTTAACATGG 
GGCATTGTTC TTGTTCCAAT CTTTGCTTTT TTTGCTGTCC TGACAGTCGC CATTTTGCTT 
ATTATGGAGG GACTGTCTGC TTTTTTACAT GCTCTCCGTC TGCACTGGGT GGAGTTCCAA 
AACAAATTTT ATTCTGGTGA AGGATACAAA TTCTTTCCCT TTTGCTTTGA GACAATGTAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031760960.1
CDS	315..2537
Translation	MSPPAPLPRDAIRMQEESEQLAKELREVSQNRQTLQERLRELLEYANILRESQRFTGPLLESEAQWKDRSEDDPLLDPAVVNKQDLRVSFMAGVIHPWRMNSFERLLWRACRGYLIVNFVEMSEPMEDLVTGESVTQIIFLISYWGEKIGEKIKKIANCFHCHIYPYADDETSRLETLRNLLVQIQDMQKVLLQTEGYLSQVLSRAASALHHWRVSVRKMKHIYLILNLCSVRERCLIGEVWCPVVDLPLLQSALTRASESSGGGGESFCHRIPCAFSPPTLIRTNKFTSGFQGIVDAYGVATYQEVNPAIFSIITFPFLFAVMFGDVGHGAIMFLFALWLVLGENDPKLRRSEDEIFSMCFGGRYLILLMGALSVYTGFVYNECFSRPAVIFNSGWSVASMARANNWTSDSINKLPPIPLNPNITGVFTAPYPFGIDPIWSLAVNRLTFLNSFKMKMSVILGVCHMAFGVCLSVFNFVHFRQIYRIFLITLPELLFLLCLFGYLVFMVVYKWIVLTAEDAEWAPSILIHFIDMFLFTQNPGNRDLYQGQQVVQTVLVIVAILCIPVLLLGDPICLLIQHRSKKDSSHQNRCSLNNGDREALLEDEITVPTGHGHGSEKFDTAEVFMHQMIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWNMVIRIGFSKLSLTWGIVLVPIFAFFAVLTVAILLIMEGLSAFLHALRLHWVEFQNKFYSGEGYKFFPFCFETMY

Target ORF information:

RefSeq Version	XM_031905100.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 (tcirg1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905100.1

ORF Insert Sequence:

ATGTCTCCGC CTGCTCCCTT ACCGAGAGAT GCCATCAGAA TGCAAGAAGA ATCTGAGCAG 
CTGGCAAAAG AGCTTAGAGA GGTGTCTCAA AATCGACAGA CTCTACAAGA GCGTTTGAGA 
GAGCTACTGG AATATGCCAA TATCCTTCGG GAGAGCCAGA GATTTACTGG GCCACTGCTG 
GAGTCTGAAG CACAATGGAA GGACAGGTCT GAGGATGATC CACTGCTGGA CCCTGCTGTA 
GTCAATAAAC AAGACCTCAG AGTCAGCTTT ATGGCAGGTG TTATTCACCC ATGGCGCATG 
AACTCATTTG AACGTTTGTT GTGGCGGGCT TGCCGTGGTT ACCTGATTGT CAACTTTGTA 
GAAATGTCAG AGCCAATGGA AGATTTGGTG ACTGGTGAGA GTGTCACTCA AATTATATTT 
CTAATTTCCT ACTGGGGCGA AAAAATTGGA GAGAAGATCA AAAAGATTGC AAACTGTTTT 
CACTGTCATA TTTATCCGTA TGCTGATGAT GAAACTTCAC GCCTTGAGAC ACTAAGAAAT 
CTTCTGGTTC AGATTCAAGA CATGCAAAAA GTGTTGTTAC AGACAGAAGG TTACCTCTCA 
CAAGTACTTT CCCGAGCTGC ATCAGCTCTG CACCACTGGA GAGTTAGTGT GCGAAAGATG 
AAACATATTT ATCTCATTCT GAACTTATGC AGTGTACGGG AGCGTTGCCT GATTGGTGAA 
GTGTGGTGTC CTGTGGTTGA TCTTCCCCTT CTTCAAAGTG CACTTACCCG GGCGTCGGAG 
AGCAGTGGAG GCGGTGGAGA GTCTTTCTGT CATCGAATAC CTTGTGCTTT TAGCCCTCCA 
ACTCTTATTC GTACCAATAA ATTCACTTCA GGTTTCCAGG GAATTGTTGA TGCTTATGGA 
GTAGCCACAT ATCAGGAGGT GAATCCAGCT ATCTTCTCAA TCATAACGTT TCCATTCCTG 
TTTGCTGTGA TGTTTGGGGA TGTGGGACAT GGTGCGATCA TGTTCCTCTT TGCTCTCTGG 
CTGGTTCTTG GTGAGAATGA TCCAAAACTT AGACGTTCTG AAGATGAGAT ATTTTCAATG 
TGTTTCGGGG GGCGTTACCT TATCCTGCTG ATGGGAGCAC TCTCGGTATA CACAGGGTTT 
GTGTATAATG AGTGTTTCAG TCGACCAGCT GTCATTTTTA ACTCAGGATG GAGTGTTGCT 
TCTATGGCAA GAGCCAACAA CTGGACGTCA GACTCAATAA ATAAATTGCC TCCAATTCCT 
CTGAACCCCA ACATTACTGG TGTGTTTACT GCACCATATC CATTTGGCAT TGATCCTATC 
TGGAGTCTTG CAGTAAATCG ACTTACTTTC TTGAACTCCT TCAAGATGAA AATGTCTGTT 
ATTTTGGGTG TTTGTCACAT GGCATTTGGT GTCTGCCTAA GTGTCTTTAA CTTTGTTCAC 
TTTAGGCAAA TATACCGCAT TTTCCTCATA ACCCTTCCGG AATTGTTGTT TCTACTTTGT 
CTCTTTGGGT ACCTGGTTTT TATGGTGGTG TATAAATGGA TTGTGCTGAC AGCTGAGGAT 
GCTGAGTGGG CTCCAAGTAT ACTTATACAT TTTATAGACA TGTTCCTGTT TACTCAGAAC 
CCTGGAAACA GAGATTTGTA CCAGGGGCAG CAAGTTGTGC AGACAGTTTT AGTGATTGTT 
GCTATACTAT GCATCCCAGT TCTGTTGCTG GGGGATCCAA TATGTCTCCT TATTCAGCAT 
CGGAGTAAAA AAGACAGCTC TCATCAGAAT CGGTGCTCGC TGAATAATGG GGATCGAGAA 
GCTCTACTGG AAGATGAGAT TACAGTACCC ACAGGACATG GACATGGCAG TGAAAAGTTT 
GATACAGCTG AAGTTTTCAT GCATCAAATG ATTCATACTA TAGAGTACTG TCTGGGCTGC 
ATATCTAATA CAGCCTCTTA CCTTCGACTG TGGGCGCTCA GCTTGGCACA TGCACAGCTG 
TCTGAGGTCC TGTGGAATAT GGTGATTAGA ATTGGATTCT CCAAGTTATC CTTAACATGG 
GGCATTGTTC TTGTTCCAAT CTTTGCTTTT TTTGCTGTCC TGACAGTCGC CATTTTGCTT 
ATTATGGAGG GACTGTCTGC TTTTTTACAT GCTCTCCGTC TGCACTGGGT GGAGTTCCAA 
AACAAATTTT ATTCTGGTGA AGGATACAAA TTCTTTCCCT TTTGCTTTGA GACAATGTAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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tcirg1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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