ddx4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ddx4
Entrez Gene ID	549577
Full Name	DEAD-box helicase 4
Synonyms	vasa,xvlg1
General protein information	Preferred Names DEAD-box helicase 4 Names probable ATP-dependent RNA helicase DDX4 DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012962855.2	XP_012818309.1	probable ATP-dependent RNA helicase DDX4 isoform X1
XM_012962855.3	XP_012818309.1	probable ATP-dependent RNA helicase DDX4 isoform X1
XM_012962857.3	XP_012818311.1	probable ATP-dependent RNA helicase DDX4 isoform X2
XM_018093500.1	XP_017948989.1	probable ATP-dependent RNA helicase DDX4 isoform X3
XM_012962857.2	XP_012818311.1	probable ATP-dependent RNA helicase DDX4 isoform X2
NM_001016823.2	NP_001016823.1	probable ATP-dependent RNA helicase DDX4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DDX4	XP_517757.3
Canis lupus familiaris (dog)	DDX4	XP_005617450.1
Gallus gallus (chicken)	DDX4	NP_990039.1
Homo sapiens (human)	DDX4	NP_077726.1
Bos taurus (cattle)	DDX4	NP_001007820.1
	DDX4	XP_001099856.2
Mus musculus (house mouse)	Ddx4	NP_001139357.1
Rattus norvegicus (Norway rat)	Ddx4	NP_001071115.1
Danio rerio (zebrafish)	vasa	NP_571132.1
Drosophila melanogaster (fruit fly)	vas	NP_001260458.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008578	XP_314684.3
Xenopus tropicalis (tropical clawed frog)	ddx4	NP_001016823.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ddx4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ddx4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa20454	NM_001016823.2 Latest version!	Xenopus tropicalis DEAD-box helicase 4 (ddx4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa27917	XM_012962857.2 Latest version!	Xenopus tropicalis DEAD-box helicase 4 (ddx4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa27916	XM_012962855.2 Latest version!	Xenopus tropicalis DEAD-box helicase 4 (ddx4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa41819	XM_018093500.1 Latest version!	Xenopus tropicalis DEAD-box helicase 4 (ddx4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OXa47909	XM_012962855.3 Latest version!	Xenopus tropicalis DEAD-box helicase 4 (ddx4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa47910	XM_012962857.3 Latest version!	Xenopus tropicalis DEAD-box helicase 4 (ddx4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ddx4 ( NM_001016823.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001016823.1 Xenopus tropicalis DEAD-box helicase 4 (ddx4), mRNA.

Price & Availability↑

CloneID	OXa20454
Clone ID Related Accession (Same CDS sequence)	NM_001016823.2
Accession Version	NM_001016823.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR855532.2. On Oct 14, 2005 this sequence version replaced NM_001016823.1. ##Evidence-Data-START## Transcript exon combination :: CR855532.2, BC157772.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGAAA ACTGGGATAC AGAAATTTCA ACTGAAAAAC CAACTTACAT CCCGAATTTC 
AGTTCCTTGG AGACAGACAA TACAGACAAT TACACTGCCT ACTCCAACAA TGATATCACC 
AATCAAAATT ATCAATCTGA AAGAAGTTTT GGAAATCGAG GAGGTTATCG ATCTGAAAGG 
GGTGGGCCTA CTAATTTTAA CCGAGGAAGT CGAATAGGAA GGGGAAGAGG GTTTGATAGT 
AATCGAAATG ACCAATATTC AGATTCTTCT GAGAGAGATG GATATAATGC TAACGAGGAT 
ACACAGAAAC CTTTTCGAGG AAGAGGTGAT TGGAATAATT CTGGTGAAGG AGAGGAGCGT 
CCTCGTGGTT TTGGGAGAGG TGGTTTTAAC AACTTTGACT CTGATACTGG TGGAAGAGGA 
CATAGAGGCG GCCGTGGTGG CAGGGGTGGA TACAAAGGAA GAAATGAAGA AGTTGGTGTT 
GAATCTGGAA AAAATCAAGA AGAGGGAACA GAAAAAGAAG TAAAAAAGGT GATCTATGTT 
CCCCTACCGC CATCTGATAG TGAAGATGAT ATATTCCGGC ACTACCAGTC TGGAATCAAT 
TTTGATAAAT ATGATGAGAT TCTTGTTGAT GTGACAGGAA AGGATGTTCC TCCAGCCATA 
TTGACTTTTG AAGAAGCTAA CTTTTGTGAG ACACTAAGCA GGAATGTCAC TAAAGCTGGA 
TATGTAAAAC TAACACCAGT GCAGAAACAC AGTATACCTA TTATATTGGC TGGTCGTGAT 
TTAATGGCTT GTGCCCAGAC CGGTTCTGGT AAAACTGCTG CTTTTCTGTT GCCAATCCTA 
AGTCATATGA TGAATGAAGG CATTACAGCT AGCCAGTTTT TACCACTTCA GGAGCCGCAA 
GCCATAATTA TTGCCCCTAC CAGAGAACTT ATTAACCAGA TATATCTCGA TGCCCGAAAA 
TTTTCATATG GAACATGTGT TCGTCCAGTA GTTGTATATG GAGGTATACA TCCTGTGCAT 
GCAATGAGGG ATGTTGAAAG AGGGTGCAAT ATACTTTGTG CAACCCCTGG AAGATTGATG 
GACATAATAG GTAGAGAAAA GATTGGCTTA AGTAAGCTAA AATATCTAGT TCTGGATGAA 
GCGGATCGCA TGTTGGATAT GGGGTTTGCC CCAGTAATGG AGAACTTAAT AGGAAGTCCA 
GGAATGCCAG CAAAAGAGGA GCGACAAACA CTGATGTTCA GTGCTACCTA TCCTGCTGAA 
ATCCAGAGGC TGGCTTCGAA GTTTCTGAAA TCTGATCATT TGTTTGTCGT GGTCGGATTA 
GTTGGAGGAG CTTGTAGTGA TGTGGAACAA ACAATCCTTG AAATACAAGA ATATCGAAAA 
AGGGACAAGC TGGTTGAAAT TCTGCAAAGC TCAGGTAATG AGCGCACAAT GATTTTTGTA 
AATACAAAAA AGAAAGCAGA TGTCATTGCT GGTTACCTTT GTCAAGAACA TTTTCCGACA 
ACAAGCATTC ACGGTGATAG AGAACAATGT CAAAGAGAGG AAGCTATCCG GGATTTCAGA 
AGTGGGAAGT GTCCTGTTAT TGTCTGTACA GCAGTTGCTG CGAGAGGCTT AGATATTGAA 
AATGTTCAAC ATGTGATAAA TTATGACGTT CCTAAGGAAA TCGACGAGTA TGTCCATAGA 
ATTGGTCGCA CTGGTCGCTG TGGTAACGTT GGAAAGGCAA CTTCATTTTT TAATGTTAAT 
GAAGACCATG TTGTTGCTCG TCCCCTTGTG AAAATCCTTA CTGATGCTCA TCAAGAAGTC 
CCTGCTTGGT TAGAAGAAAT TGCCTTTGGT GGCCATGGAG CTTTGAACAG CTTATATGCA 
GCAGACTCTA TGGGTGGAGA GGCTGGAGAA AAGTACGTCA GTGCTCCTTC ATCTGCACAA 
GAGGAGGAGG CCAGCTGGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001016823.1
CDS	85..2028
Translation	MEENWDTEISTEKPTYIPNFSSLETDNTDNYTAYSNNDITNQNYQSERSFGNRGGYRSERGGPTNFNRGSRIGRGRGFDSNRNDQYSDSSERDGYNANEDTQKPFRGRGDWNNSGEGEERPRGFGRGGFNNFDSDTGGRGHRGGRGGRGGYKGRNEEVGVESGKNQEEGTEKEVKKVIYVPLPPSDSEDDIFRHYQSGINFDKYDEILVDVTGKDVPPAILTFEEANFCETLSRNVTKAGYVKLTPVQKHSIPIILAGRDLMACAQTGSGKTAAFLLPILSHMMNEGITASQFLPLQEPQAIIIAPTRELINQIYLDARKFSYGTCVRPVVVYGGIHPVHAMRDVERGCNILCATPGRLMDIIGREKIGLSKLKYLVLDEADRMLDMGFAPVMENLIGSPGMPAKEERQTLMFSATYPAEIQRLASKFLKSDHLFVVVGLVGGACSDVEQTILEIQEYRKRDKLVEILQSSGNERTMIFVNTKKKADVIAGYLCQEHFPTTSIHGDREQCQREEAIRDFRSGKCPVIVCTAVAARGLDIENVQHVINYDVPKEIDEYVHRIGRTGRCGNVGKATSFFNVNEDHVVARPLVKILTDAHQEVPAWLEEIAFGGHGALNSLYAADSMGGEAGEKYVSAPSSAQEEEASWD

Target ORF information:

RefSeq Version	NM_001016823.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAD-box helicase 4 (ddx4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001016823.2

ORF Insert Sequence:

ATGGAGGAAA ACTGGGATAC AGAAATTTCA ACTGAAAAAC CAACTTACAT CCCGAATTTC 
AGTTCCTTGG AGACAGACAA TACAGACAAT TACACTGCCT ACTCCAACAA TGATATCACC 
AATCAAAATT ATCAATCTGA AAGAAGTTTT GGAAATCGAG GAGGTTATCG ATCTGAAAGG 
GGTGGGCCTA CTAATTTTAA CCGAGGAAGT CGAATAGGAA GGGGAAGAGG GTTTGATAGT 
AATCGAAATG ACCAATATTC AGATTCTTCT GAGAGAGATG GATATAATGC TAACGAGGAT 
ACACAGAAAC CTTTTCGAGG AAGAGGTGAT TGGAATAATT CTGGTGAAGG AGAGGAGCGT 
CCTCGTGGTT TTGGGAGAGG TGGTTTTAAC AACTTTGACT CTGATACTGG TGGAAGAGGA 
CATAGAGGCG GCCGTGGTGG CAGGGGTGGA TACAAAGGAA GAAATGAAGA AGTTGGTGTT 
GAATCTGGAA AAAATCAAGA AGAGGGAACA GAAAAAGAAG TAAAAAAGGT GATCTATGTT 
CCCCTACCGC CATCTGATAG TGAAGATGAT ATATTCCGGC ACTACCAGTC TGGAATCAAT 
TTTGATAAAT ATGATGAGAT TCTTGTTGAT GTGACAGGAA AGGATGTTCC TCCAGCCATA 
TTGACTTTTG AAGAAGCTAA CTTTTGTGAG ACACTAAGCA GGAATGTCAC TAAAGCTGGA 
TATGTAAAAC TAACACCAGT GCAGAAACAC AGTATACCTA TTATATTGGC TGGTCGTGAT 
TTAATGGCTT GTGCCCAGAC CGGTTCTGGT AAAACTGCTG CTTTTCTGTT GCCAATCCTA 
AGTCATATGA TGAATGAAGG CATTACAGCT AGCCAGTTTT TACCACTTCA GGAGCCGCAA 
GCCATAATTA TTGCCCCTAC CAGAGAACTT ATTAACCAGA TATATCTCGA TGCCCGAAAA 
TTTTCATATG GAACATGTGT TCGTCCAGTA GTTGTATATG GAGGTATACA TCCTGTGCAT 
GCAATGAGGG ATGTTGAAAG AGGGTGCAAT ATACTTTGTG CAACCCCTGG AAGATTGATG 
GACATAATAG GTAGAGAAAA GATTGGCTTA AGTAAGCTAA AATATCTAGT TCTGGATGAA 
GCGGATCGCA TGTTGGATAT GGGGTTTGCC CCAGTAATGG AGAACTTAAT AGGAAGTCCA 
GGAATGCCAG CAAAAGAGGA GCGACAAACA CTGATGTTCA GTGCTACCTA TCCTGCTGAA 
ATCCAGAGGC TGGCTTCGAA GTTTCTGAAA TCTGATCATT TGTTTGTCGT GGTCGGATTA 
GTTGGAGGAG CTTGTAGTGA TGTGGAACAA ACAATCCTTG AAATACAAGA ATATCGAAAA 
AGGGACAAGC TGGTTGAAAT TCTGCAAAGC TCAGGTAATG AGCGCACAAT GATTTTTGTA 
AATACAAAAA AGAAAGCAGA TGTCATTGCT GGTTACCTTT GTCAAGAACA TTTTCCGACA 
ACAAGCATTC ACGGTGATAG AGAACAATGT CAAAGAGAGG AAGCTATCCG GGATTTCAGA 
AGTGGGAAGT GTCCTGTTAT TGTCTGTACA GCAGTTGCTG CGAGAGGCTT AGATATTGAA 
AATGTTCAAC ATGTGATAAA TTATGACGTT CCTAAGGAAA TCGACGAGTA TGTCCATAGA 
ATTGGTCGCA CTGGTCGCTG TGGTAACGTT GGAAAGGCAA CTTCATTTTT TAATGTTAAT 
GAAGACCATG TTGTTGCTCG TCCCCTTGTG AAAATCCTTA CTGATGCTCA TCAAGAAGTC 
CCTGCTTGGT TAGAAGAAAT TGCCTTTGGT GGCCATGGAG CTTTGAACAG CTTATATGCA 
GCAGACTCTA TGGGTGGAGA GGCTGGAGAA AAGTACGTCA GTGCTCCTTC ATCTGCACAA 
GAGGAGGAGG CCAGCTGGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx4 ( XM_012962857.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818311.1 Xenopus tropicalis DEAD-box helicase 4 (ddx4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa27917
Clone ID Related Accession (Same CDS sequence)	XM_012962857.2
Accession Version	XM_012962857.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962857.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA ACTGGGATAC AGAAATTTCA ACTGAAAAAC CAACTTACAT CCCGAATTTC 
AGTTCCTTGG AGACAGACAA TACAGACAAT TACACTGCCT ACTCCAACAA TGATATCACC 
AATCAAAATT ATCAATCTGA AAGAAGTTTT GGAAATCGAG GAGGTTATCG ATCTGAAAGG 
GGTGGGCCTA CTAATTTTAA CCGAGGAAGT CGAATAGGAA GGGGAAGAGG GTTTGATAGT 
AATCGAAATG ACCAATATTC AGATTCTTCT GAGAGAGATG GATATAATGC TAACGAGGAT 
ACACAGAAAC CTTTTCGAGG AAGAGGTGGA TTTAGAAATG AAAATGGACA GCAAAGAAAC 
TTCGATAGAG GAGATTGGAA TAATTCTGGT GAAGGAGAGG AGCGTCCTCG TGGTTTTGGG 
AGAGGTGGTT TTAACAACTT TGACTCTGAT ACTGGTGGAA GAGGACATAG AGGCGGCCGT 
GGTGGCAGGG GTGGATACAA AGGAAGAAAT GAAGAAGTTG GTGTTGAATC TGGAAAAAAT 
CAAGAAGAGG GAACAGAAAA AGAAGTAAAA AAGGTGATCT ATGTTCCCCT ACCGCCATCT 
GATAGTGAAG ATGATATATT CCGGCACTAC CAGTCTGGAA TCAATTTTGA TAAATATGAT 
GAGATTCTTG TTGATGTGAC AGGAAAGGAT GTTCCTCCAG CCATATTGAC TTTTGAAGAA 
GCTAACTTTT GTGAGACACT AAGCAGGAAT GTCACTAAAG CTGGATATGT AAAACTAACA 
CCAGTGCAGA AACACAGTAT ACCTATTATA TTGGCTGGTC GTGATTTAAT GGCTTGTGCC 
CAGACCGGTT CTGGTAAAAC TGCTGCTTTT CTGTTGCCAA TCCTAAGTCA TATGATGAAT 
GAAGGCATTA CAGCTAGCCA GTTTTTACCA CTTCAGGAGC CGCAAGCCAT AATTATTGCC 
CCTACCAGAG AACTTATTAA CCAGATATAT CTCGATGCCC GAAAATTTTC ATATGGAACC 
TGTGTTCGTC CAGTAGTTGT ATATGGAGGT ATACATCCTG TGCATGCAAT GAGGGATGTT 
GAAAGAGGGT GCAATATACT TTGTGCAACC CCTGGAAGAT TGATGGACAT AATAGGTAGA 
GAAAAGATTG GCTTAAGTAA GCTAAAATAT CTAGTTCTGG ATGAAGCGGA TCGCATGTTG 
GATATGGGAT TTGCCCCAGT AATGGAGAAC TTAATAGGAA GTCCAGGAAT GCCAGCAAAA 
GAGGAGCGAC AAACACTGAT GTTCAGTGCT ACCTATCCTG CTGAAATCCA GAGGCTGGCT 
TCGAAGTTTC TGAAATCTGA TCATTTGTTT GTCGTGGTCG GATTAGTTGG AGGAGCTTGT 
AGTGATGTGG AACAAACAAT CCTTGAAATA CAAGAATATC GAAAAAGGGA CAAGCTGGTT 
GAAATTCTGC AAAGCTCAGG TAATGAGCGC ACAATGATTT TTGTAAATAC AAAAAAGAAA 
GCAGATGTCA TTGCTGGTTA CCTTTGTCAA GAACATTTTC CGACAACAAG CATTCACGGT 
GATAGAGAAC AATGTCAAAG AGAGGAAGCT ATCCGGGATT TCAGAAGTGG GAAGTGTCCT 
GTTATTGTCT GTACAGCAGT TGCTGCGAGA GGTTTAGATA TTGAAAATGT TCAACATGTG 
ATAAATTATG ACGTTCCTAA GGAAATCGAC GAGTATGTCC ATAGAATTGG TCGCACTGGT 
CGCTGTGGTA ACGTTGGAAA GGCAACTTCA TTTTTTAATG TTAATGAAGA CCATGTTGTT 
GCTCGTCCCC TTGTGAAAAT CCTTACTGAT GCTCATCAAG AAGTCCCTGC TTGGTTAGAA 
GAAATTGCCT TTGGTGGCCA TGGAGCTTTG AACAGCTTAT ATGCAGCAGA CTCTATGGGT 
GGAGAGGCTG GAGAAAAGTA CGTCAGTGCT CCTTCATCTG CACAAGAGGA GGAGGCCAGC 
TGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818311.1
CDS	209..2197
Translation	MEENWDTEISTEKPTYIPNFSSLETDNTDNYTAYSNNDITNQNYQSERSFGNRGGYRSERGGPTNFNRGSRIGRGRGFDSNRNDQYSDSSERDGYNANEDTQKPFRGRGGFRNENGQQRNFDRGDWNNSGEGEERPRGFGRGGFNNFDSDTGGRGHRGGRGGRGGYKGRNEEVGVESGKNQEEGTEKEVKKVIYVPLPPSDSEDDIFRHYQSGINFDKYDEILVDVTGKDVPPAILTFEEANFCETLSRNVTKAGYVKLTPVQKHSIPIILAGRDLMACAQTGSGKTAAFLLPILSHMMNEGITASQFLPLQEPQAIIIAPTRELINQIYLDARKFSYGTCVRPVVVYGGIHPVHAMRDVERGCNILCATPGRLMDIIGREKIGLSKLKYLVLDEADRMLDMGFAPVMENLIGSPGMPAKEERQTLMFSATYPAEIQRLASKFLKSDHLFVVVGLVGGACSDVEQTILEIQEYRKRDKLVEILQSSGNERTMIFVNTKKKADVIAGYLCQEHFPTTSIHGDREQCQREEAIRDFRSGKCPVIVCTAVAARGLDIENVQHVINYDVPKEIDEYVHRIGRTGRCGNVGKATSFFNVNEDHVVARPLVKILTDAHQEVPAWLEEIAFGGHGALNSLYAADSMGGEAGEKYVSAPSSAQEEEASWD

Target ORF information:

RefSeq Version	XM_012962857.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAD-box helicase 4 (ddx4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962857.2

ORF Insert Sequence:

ATGGAGGAAA ACTGGGATAC AGAAATTTCA ACTGAAAAAC CAACTTACAT CCCGAATTTC 
AGTTCCTTGG AGACAGACAA TACAGACAAT TACACTGCCT ACTCCAACAA TGATATCACC 
AATCAAAATT ATCAATCTGA AAGAAGTTTT GGAAATCGAG GAGGTTATCG ATCTGAAAGG 
GGTGGGCCTA CTAATTTTAA CCGAGGAAGT CGAATAGGAA GGGGAAGAGG GTTTGATAGT 
AATCGAAATG ACCAATATTC AGATTCTTCT GAGAGAGATG GATATAATGC TAACGAGGAT 
ACACAGAAAC CTTTTCGAGG AAGAGGTGGA TTTAGAAATG AAAATGGACA GCAAAGAAAC 
TTCGATAGAG GAGATTGGAA TAATTCTGGT GAAGGAGAGG AGCGTCCTCG TGGTTTTGGG 
AGAGGTGGTT TTAACAACTT TGACTCTGAT ACTGGTGGAA GAGGACATAG AGGCGGCCGT 
GGTGGCAGGG GTGGATACAA AGGAAGAAAT GAAGAAGTTG GTGTTGAATC TGGAAAAAAT 
CAAGAAGAGG GAACAGAAAA AGAAGTAAAA AAGGTGATCT ATGTTCCCCT ACCGCCATCT 
GATAGTGAAG ATGATATATT CCGGCACTAC CAGTCTGGAA TCAATTTTGA TAAATATGAT 
GAGATTCTTG TTGATGTGAC AGGAAAGGAT GTTCCTCCAG CCATATTGAC TTTTGAAGAA 
GCTAACTTTT GTGAGACACT AAGCAGGAAT GTCACTAAAG CTGGATATGT AAAACTAACA 
CCAGTGCAGA AACACAGTAT ACCTATTATA TTGGCTGGTC GTGATTTAAT GGCTTGTGCC 
CAGACCGGTT CTGGTAAAAC TGCTGCTTTT CTGTTGCCAA TCCTAAGTCA TATGATGAAT 
GAAGGCATTA CAGCTAGCCA GTTTTTACCA CTTCAGGAGC CGCAAGCCAT AATTATTGCC 
CCTACCAGAG AACTTATTAA CCAGATATAT CTCGATGCCC GAAAATTTTC ATATGGAACC 
TGTGTTCGTC CAGTAGTTGT ATATGGAGGT ATACATCCTG TGCATGCAAT GAGGGATGTT 
GAAAGAGGGT GCAATATACT TTGTGCAACC CCTGGAAGAT TGATGGACAT AATAGGTAGA 
GAAAAGATTG GCTTAAGTAA GCTAAAATAT CTAGTTCTGG ATGAAGCGGA TCGCATGTTG 
GATATGGGAT TTGCCCCAGT AATGGAGAAC TTAATAGGAA GTCCAGGAAT GCCAGCAAAA 
GAGGAGCGAC AAACACTGAT GTTCAGTGCT ACCTATCCTG CTGAAATCCA GAGGCTGGCT 
TCGAAGTTTC TGAAATCTGA TCATTTGTTT GTCGTGGTCG GATTAGTTGG AGGAGCTTGT 
AGTGATGTGG AACAAACAAT CCTTGAAATA CAAGAATATC GAAAAAGGGA CAAGCTGGTT 
GAAATTCTGC AAAGCTCAGG TAATGAGCGC ACAATGATTT TTGTAAATAC AAAAAAGAAA 
GCAGATGTCA TTGCTGGTTA CCTTTGTCAA GAACATTTTC CGACAACAAG CATTCACGGT 
GATAGAGAAC AATGTCAAAG AGAGGAAGCT ATCCGGGATT TCAGAAGTGG GAAGTGTCCT 
GTTATTGTCT GTACAGCAGT TGCTGCGAGA GGTTTAGATA TTGAAAATGT TCAACATGTG 
ATAAATTATG ACGTTCCTAA GGAAATCGAC GAGTATGTCC ATAGAATTGG TCGCACTGGT 
CGCTGTGGTA ACGTTGGAAA GGCAACTTCA TTTTTTAATG TTAATGAAGA CCATGTTGTT 
GCTCGTCCCC TTGTGAAAAT CCTTACTGAT GCTCATCAAG AAGTCCCTGC TTGGTTAGAA 
GAAATTGCCT TTGGTGGCCA TGGAGCTTTG AACAGCTTAT ATGCAGCAGA CTCTATGGGT 
GGAGAGGCTG GAGAAAAGTA CGTCAGTGCT CCTTCATCTG CACAAGAGGA GGAGGCCAGC 
TGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx4 ( XM_012962855.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818309.1 Xenopus tropicalis DEAD-box helicase 4 (ddx4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa27916
Clone ID Related Accession (Same CDS sequence)	XM_012962855.2
Accession Version	XM_012962855.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962855.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA ACTGGGATAC AGAAATTTCA ACTGAAAAAC CAACTTACAT CCCGAATTTC 
AGTTCCTTGG AGACAGACAA TACAGACAAT TACACTGCCT ACTCCAACAA TGATATCACC 
AATCAAAATT ATCAATCTGA AAGAAGTTTT GGAAATCGAG GAGGTTATCG ATCTGAAAGG 
GGTGGGCCTA CTAATTTTAA CCGAGGAAGT CGAATAGGAA GGGGAAGAGG GTTTGATAGT 
AATCGAAATG ACCAATATTC AGATTCTTCT GAGAGAGATG GATATAATGC TAACGAGGAT 
ACACAGAAAC CTTTTCGAGG AAGAGGTGGG TTCAGAAATG AGAATGGACA GCGGCGAGGT 
TTTGGAGACA GAGGAGGATT TAGAAATGAA AATGGACAGC AAAGAAACTT CGATAGAGGA 
GATTGGAATA ATTCTGGTGA AGGAGAGGAG CGTCCTCGTG GTTTTGGGAG AGGTGGTTTT 
AACAACTTTG ACTCTGATAC TGGTGGAAGA GGACATAGAG GCGGCCGTGG TGGCAGGGGT 
GGATACAAAG GAAGAAATGA AGAAGTTGGT GTTGAATCTG GAAAAAATCA AGAAGAGGGA 
ACAGAAAAAG AAGTAAAAAA GGTGATCTAT GTTCCCCTAC CGCCATCTGA TAGTGAAGAT 
GATATATTCC GGCACTACCA GTCTGGAATC AATTTTGATA AATATGATGA GATTCTTGTT 
GATGTGACAG GAAAGGATGT TCCTCCAGCC ATATTGACTT TTGAAGAAGC TAACTTTTGT 
GAGACACTAA GCAGGAATGT CACTAAAGCT GGATATGTAA AACTAACACC AGTGCAGAAA 
CACAGTATAC CTATTATATT GGCTGGTCGT GATTTAATGG CTTGTGCCCA GACCGGTTCT 
GGTAAAACTG CTGCTTTTCT GTTGCCAATC CTAAGTCATA TGATGAATGA AGGCATTACA 
GCTAGCCAGT TTTTACCACT TCAGGAGCCG CAAGCCATAA TTATTGCCCC TACCAGAGAA 
CTTATTAACC AGATATATCT CGATGCCCGA AAATTTTCAT ATGGAACCTG TGTTCGTCCA 
GTAGTTGTAT ATGGAGGTAT ACATCCTGTG CATGCAATGA GGGATGTTGA AAGAGGGTGC 
AATATACTTT GTGCAACCCC TGGAAGATTG ATGGACATAA TAGGTAGAGA AAAGATTGGC 
TTAAGTAAGC TAAAATATCT AGTTCTGGAT GAAGCGGATC GCATGTTGGA TATGGGATTT 
GCCCCAGTAA TGGAGAACTT AATAGGAAGT CCAGGAATGC CAGCAAAAGA GGAGCGACAA 
ACACTGATGT TCAGTGCTAC CTATCCTGCT GAAATCCAGA GGCTGGCTTC GAAGTTTCTG 
AAATCTGATC ATTTGTTTGT CGTGGTCGGA TTAGTTGGAG GAGCTTGTAG TGATGTGGAA 
CAAACAATCC TTGAAATACA AGAATATCGA AAAAGGGACA AGCTGGTTGA AATTCTGCAA 
AGCTCAGGTA ATGAGCGCAC AATGATTTTT GTAAATACAA AAAAGAAAGC AGATGTCATT 
GCTGGTTACC TTTGTCAAGA ACATTTTCCG ACAACAAGCA TTCACGGTGA TAGAGAACAA 
TGTCAAAGAG AGGAAGCTAT CCGGGATTTC AGAAGTGGGA AGTGTCCTGT TATTGTCTGT 
ACAGCAGTTG CTGCGAGAGG TTTAGATATT GAAAATGTTC AACATGTGAT AAATTATGAC 
GTTCCTAAGG AAATCGACGA GTATGTCCAT AGAATTGGTC GCACTGGTCG CTGTGGTAAC 
GTTGGAAAGG CAACTTCATT TTTTAATGTT AATGAAGACC ATGTTGTTGC TCGTCCCCTT 
GTGAAAATCC TTACTGATGC TCATCAAGAA GTCCCTGCTT GGTTAGAAGA AATTGCCTTT 
GGTGGCCATG GAGCTTTGAA CAGCTTATAT GCAGCAGACT CTATGGGTGG AGAGGCTGGA 
GAAAAGTACG TCAGTGCTCC TTCATCTGCA CAAGAGGAGG AGGCCAGCTG GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818309.1
CDS	209..2245
Translation	MEENWDTEISTEKPTYIPNFSSLETDNTDNYTAYSNNDITNQNYQSERSFGNRGGYRSERGGPTNFNRGSRIGRGRGFDSNRNDQYSDSSERDGYNANEDTQKPFRGRGGFRNENGQRRGFGDRGGFRNENGQQRNFDRGDWNNSGEGEERPRGFGRGGFNNFDSDTGGRGHRGGRGGRGGYKGRNEEVGVESGKNQEEGTEKEVKKVIYVPLPPSDSEDDIFRHYQSGINFDKYDEILVDVTGKDVPPAILTFEEANFCETLSRNVTKAGYVKLTPVQKHSIPIILAGRDLMACAQTGSGKTAAFLLPILSHMMNEGITASQFLPLQEPQAIIIAPTRELINQIYLDARKFSYGTCVRPVVVYGGIHPVHAMRDVERGCNILCATPGRLMDIIGREKIGLSKLKYLVLDEADRMLDMGFAPVMENLIGSPGMPAKEERQTLMFSATYPAEIQRLASKFLKSDHLFVVVGLVGGACSDVEQTILEIQEYRKRDKLVEILQSSGNERTMIFVNTKKKADVIAGYLCQEHFPTTSIHGDREQCQREEAIRDFRSGKCPVIVCTAVAARGLDIENVQHVINYDVPKEIDEYVHRIGRTGRCGNVGKATSFFNVNEDHVVARPLVKILTDAHQEVPAWLEEIAFGGHGALNSLYAADSMGGEAGEKYVSAPSSAQEEEASWD

Target ORF information:

RefSeq Version	XM_012962855.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAD-box helicase 4 (ddx4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962855.2

ORF Insert Sequence:

ATGGAGGAAA ACTGGGATAC AGAAATTTCA ACTGAAAAAC CAACTTACAT CCCGAATTTC 
AGTTCCTTGG AGACAGACAA TACAGACAAT TACACTGCCT ACTCCAACAA TGATATCACC 
AATCAAAATT ATCAATCTGA AAGAAGTTTT GGAAATCGAG GAGGTTATCG ATCTGAAAGG 
GGTGGGCCTA CTAATTTTAA CCGAGGAAGT CGAATAGGAA GGGGAAGAGG GTTTGATAGT 
AATCGAAATG ACCAATATTC AGATTCTTCT GAGAGAGATG GATATAATGC TAACGAGGAT 
ACACAGAAAC CTTTTCGAGG AAGAGGTGGG TTCAGAAATG AGAATGGACA GCGGCGAGGT 
TTTGGAGACA GAGGAGGATT TAGAAATGAA AATGGACAGC AAAGAAACTT CGATAGAGGA 
GATTGGAATA ATTCTGGTGA AGGAGAGGAG CGTCCTCGTG GTTTTGGGAG AGGTGGTTTT 
AACAACTTTG ACTCTGATAC TGGTGGAAGA GGACATAGAG GCGGCCGTGG TGGCAGGGGT 
GGATACAAAG GAAGAAATGA AGAAGTTGGT GTTGAATCTG GAAAAAATCA AGAAGAGGGA 
ACAGAAAAAG AAGTAAAAAA GGTGATCTAT GTTCCCCTAC CGCCATCTGA TAGTGAAGAT 
GATATATTCC GGCACTACCA GTCTGGAATC AATTTTGATA AATATGATGA GATTCTTGTT 
GATGTGACAG GAAAGGATGT TCCTCCAGCC ATATTGACTT TTGAAGAAGC TAACTTTTGT 
GAGACACTAA GCAGGAATGT CACTAAAGCT GGATATGTAA AACTAACACC AGTGCAGAAA 
CACAGTATAC CTATTATATT GGCTGGTCGT GATTTAATGG CTTGTGCCCA GACCGGTTCT 
GGTAAAACTG CTGCTTTTCT GTTGCCAATC CTAAGTCATA TGATGAATGA AGGCATTACA 
GCTAGCCAGT TTTTACCACT TCAGGAGCCG CAAGCCATAA TTATTGCCCC TACCAGAGAA 
CTTATTAACC AGATATATCT CGATGCCCGA AAATTTTCAT ATGGAACCTG TGTTCGTCCA 
GTAGTTGTAT ATGGAGGTAT ACATCCTGTG CATGCAATGA GGGATGTTGA AAGAGGGTGC 
AATATACTTT GTGCAACCCC TGGAAGATTG ATGGACATAA TAGGTAGAGA AAAGATTGGC 
TTAAGTAAGC TAAAATATCT AGTTCTGGAT GAAGCGGATC GCATGTTGGA TATGGGATTT 
GCCCCAGTAA TGGAGAACTT AATAGGAAGT CCAGGAATGC CAGCAAAAGA GGAGCGACAA 
ACACTGATGT TCAGTGCTAC CTATCCTGCT GAAATCCAGA GGCTGGCTTC GAAGTTTCTG 
AAATCTGATC ATTTGTTTGT CGTGGTCGGA TTAGTTGGAG GAGCTTGTAG TGATGTGGAA 
CAAACAATCC TTGAAATACA AGAATATCGA AAAAGGGACA AGCTGGTTGA AATTCTGCAA 
AGCTCAGGTA ATGAGCGCAC AATGATTTTT GTAAATACAA AAAAGAAAGC AGATGTCATT 
GCTGGTTACC TTTGTCAAGA ACATTTTCCG ACAACAAGCA TTCACGGTGA TAGAGAACAA 
TGTCAAAGAG AGGAAGCTAT CCGGGATTTC AGAAGTGGGA AGTGTCCTGT TATTGTCTGT 
ACAGCAGTTG CTGCGAGAGG TTTAGATATT GAAAATGTTC AACATGTGAT AAATTATGAC 
GTTCCTAAGG AAATCGACGA GTATGTCCAT AGAATTGGTC GCACTGGTCG CTGTGGTAAC 
GTTGGAAAGG CAACTTCATT TTTTAATGTT AATGAAGACC ATGTTGTTGC TCGTCCCCTT 
GTGAAAATCC TTACTGATGC TCATCAAGAA GTCCCTGCTT GGTTAGAAGA AATTGCCTTT 
GGTGGCCATG GAGCTTTGAA CAGCTTATAT GCAGCAGACT CTATGGGTGG AGAGGCTGGA 
GAAAAGTACG TCAGTGCTCC TTCATCTGCA CAAGAGGAGG AGGCCAGCTG GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx4 ( XM_018093500.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948989.1 Xenopus tropicalis DEAD-box helicase 4 (ddx4), transcript variant X3, mRNA.

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CloneID	OXa41819
Clone ID Related Accession (Same CDS sequence)	XM_018093500.1
Accession Version	XM_018093500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA ACTGGGATAC AGAAATTTCA ACTGAAAAAC CAACTTACAT CCCGAATTTC 
AGTTCCTTGG AGACAGACAA TACAGACAAT TACACTGCCT ACTGTTATCG ATCTGAAAGG 
GGTGGGCCTA CTAATTTTAA CCGAGGAAGT CGAATAGGAA GGGGAAGAGG GTTTGATAGT 
AATCGAAATG ACCAATATTC AGATTCTTCT GAGAGAGATG GATATAATGC TAACGAGGAT 
ACACAGAAAC CTTTTCGAGG AAGAGGTGGG TTCAGAAATG AGAATGGACA GCGGCGAGGT 
TTTGGAGACA GAGGAGGATT TAGAAATGAA AATGGACAGC AAAGAAACTT CGATAGAGGA 
GATTGGAATA ATTCTGGTGA AGGAGAGGAG CGTCCTCGTG GTTTTGGGAG AGGTGGTTTT 
AACAACTTTG ACTCTGATAC TGGTGGAAGA GGACATAGAG GCGGCCGTGG TGGCAGGGGT 
GGATACAAAG GAAGAAATGA AGAAGTTGGT GTTGAATCTG GAAAAAATCA AGAAGAGGGA 
ACAGAAAAAG AAGTAAAAAA GGTGATCTAT GTTCCCCTAC CGCCATCTGA TAGTGAAGAT 
GATATATTCC GGCACTACCA GTCTGGAATC AATTTTGATA AATATGATGA GATTCTTGTT 
GATGTGACAG GAAAGGATGT TCCTCCAGCC ATATTGACTT TTGAAGAAGC TAACTTTTGT 
GAGACACTAA GCAGGAATGT CACTAAAGCT GGATATGTAA AACTAACACC AGTGCAGAAA 
CACAGTATAC CTATTATATT GGCTGGTCGT GATTTAATGG CTTGTGCCCA GACCGGTTCT 
GGTAAAACTG CTGCTTTTCT GTTGCCAATC CTAAGTCATA TGATGAATGA AGGCATTACA 
GCTAGCCAGT TTTTACCACT TCAGGAGCCG CAAGCCATAA TTATTGCCCC TACCAGAGAA 
CTTATTAACC AGATATATCT CGATGCCCGA AAATTTTCAT ATGGAACCTG TGTTCGTCCA 
GTAGTTGTAT ATGGAGGTAT ACATCCTGTG CATGCAATGA GGGATGTTGA AAGAGGGTGC 
AATATACTTT GTGCAACCCC TGGAAGATTG ATGGACATAA TAGGTAGAGA AAAGATTGGC 
TTAAGTAAGC TAAAATATCT AGTTCTGGAT GAAGCGGATC GCATGTTGGA TATGGGATTT 
GCCCCAGTAA TGGAGAACTT AATAGGAAGT CCAGGAATGC CAGCAAAAGA GGAGCGACAA 
ACACTGATGT TCAGTGCTAC CTATCCTGCT GAAATCCAGA GGCTGGCTTC GAAGTTTCTG 
AAATCTGATC ATTTGTTTGT CGTGGTCGGA TTAGTTGGAG GAGCTTGTAG TGATGTGGAA 
CAAACAATCC TTGAAATACA AGAATATCGA AAAAGGGACA AGCTGGTTGA AATTCTGCAA 
AGCTCAGGTA ATGAGCGCAC AATGATTTTT GTAAATACAA AAAAGAAAGC AGATGTCATT 
GCTGGTTACC TTTGTCAAGA ACATTTTCCG ACAACAAGCA TTCACGGTGA TAGAGAACAA 
TGTCAAAGAG AGGAAGCTAT CCGGGATTTC AGAAGTGGGA AGTGTCCTGT TATTGTCTGT 
ACAGCAGTTG CTGCGAGAGG TTTAGATATT GAAAATGTTC AACATGTGAT AAATTATGAC 
GTTCCTAAGG AAATCGACGA GTATGTCCAT AGAATTGGTC GCACTGGTCG CTGTGGTAAC 
GTTGGAAAGG CAACTTCATT TTTTAATGTT AATGAAGACC ATGTTGTTGC TCGTCCCCTT 
GTGAAAATCC TTACTGATGC TCATCAAGAA GTCCCTGCTT GGTTAGAAGA AATTGCCTTT 
GGTGGCCATG GAGCTTTGAA CAGCTTATAT GCAGCAGACT CTATGGGTGG AGAGGCTGGA 
GAAAAGTACG TCAGTGCTCC TTCATCTGCA CAAGAGGAGG AGGCCAGCTG GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948989.1
CDS	209..2185
Translation	MEENWDTEISTEKPTYIPNFSSLETDNTDNYTAYCYRSERGGPTNFNRGSRIGRGRGFDSNRNDQYSDSSERDGYNANEDTQKPFRGRGGFRNENGQRRGFGDRGGFRNENGQQRNFDRGDWNNSGEGEERPRGFGRGGFNNFDSDTGGRGHRGGRGGRGGYKGRNEEVGVESGKNQEEGTEKEVKKVIYVPLPPSDSEDDIFRHYQSGINFDKYDEILVDVTGKDVPPAILTFEEANFCETLSRNVTKAGYVKLTPVQKHSIPIILAGRDLMACAQTGSGKTAAFLLPILSHMMNEGITASQFLPLQEPQAIIIAPTRELINQIYLDARKFSYGTCVRPVVVYGGIHPVHAMRDVERGCNILCATPGRLMDIIGREKIGLSKLKYLVLDEADRMLDMGFAPVMENLIGSPGMPAKEERQTLMFSATYPAEIQRLASKFLKSDHLFVVVGLVGGACSDVEQTILEIQEYRKRDKLVEILQSSGNERTMIFVNTKKKADVIAGYLCQEHFPTTSIHGDREQCQREEAIRDFRSGKCPVIVCTAVAARGLDIENVQHVINYDVPKEIDEYVHRIGRTGRCGNVGKATSFFNVNEDHVVARPLVKILTDAHQEVPAWLEEIAFGGHGALNSLYAADSMGGEAGEKYVSAPSSAQEEEASWD

Target ORF information:

RefSeq Version	XM_018093500.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAD-box helicase 4 (ddx4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093500.1

ORF Insert Sequence:

ATGGAGGAAA ACTGGGATAC AGAAATTTCA ACTGAAAAAC CAACTTACAT CCCGAATTTC 
AGTTCCTTGG AGACAGACAA TACAGACAAT TACACTGCCT ACTGTTATCG ATCTGAAAGG 
GGTGGGCCTA CTAATTTTAA CCGAGGAAGT CGAATAGGAA GGGGAAGAGG GTTTGATAGT 
AATCGAAATG ACCAATATTC AGATTCTTCT GAGAGAGATG GATATAATGC TAACGAGGAT 
ACACAGAAAC CTTTTCGAGG AAGAGGTGGG TTCAGAAATG AGAATGGACA GCGGCGAGGT 
TTTGGAGACA GAGGAGGATT TAGAAATGAA AATGGACAGC AAAGAAACTT CGATAGAGGA 
GATTGGAATA ATTCTGGTGA AGGAGAGGAG CGTCCTCGTG GTTTTGGGAG AGGTGGTTTT 
AACAACTTTG ACTCTGATAC TGGTGGAAGA GGACATAGAG GCGGCCGTGG TGGCAGGGGT 
GGATACAAAG GAAGAAATGA AGAAGTTGGT GTTGAATCTG GAAAAAATCA AGAAGAGGGA 
ACAGAAAAAG AAGTAAAAAA GGTGATCTAT GTTCCCCTAC CGCCATCTGA TAGTGAAGAT 
GATATATTCC GGCACTACCA GTCTGGAATC AATTTTGATA AATATGATGA GATTCTTGTT 
GATGTGACAG GAAAGGATGT TCCTCCAGCC ATATTGACTT TTGAAGAAGC TAACTTTTGT 
GAGACACTAA GCAGGAATGT CACTAAAGCT GGATATGTAA AACTAACACC AGTGCAGAAA 
CACAGTATAC CTATTATATT GGCTGGTCGT GATTTAATGG CTTGTGCCCA GACCGGTTCT 
GGTAAAACTG CTGCTTTTCT GTTGCCAATC CTAAGTCATA TGATGAATGA AGGCATTACA 
GCTAGCCAGT TTTTACCACT TCAGGAGCCG CAAGCCATAA TTATTGCCCC TACCAGAGAA 
CTTATTAACC AGATATATCT CGATGCCCGA AAATTTTCAT ATGGAACCTG TGTTCGTCCA 
GTAGTTGTAT ATGGAGGTAT ACATCCTGTG CATGCAATGA GGGATGTTGA AAGAGGGTGC 
AATATACTTT GTGCAACCCC TGGAAGATTG ATGGACATAA TAGGTAGAGA AAAGATTGGC 
TTAAGTAAGC TAAAATATCT AGTTCTGGAT GAAGCGGATC GCATGTTGGA TATGGGATTT 
GCCCCAGTAA TGGAGAACTT AATAGGAAGT CCAGGAATGC CAGCAAAAGA GGAGCGACAA 
ACACTGATGT TCAGTGCTAC CTATCCTGCT GAAATCCAGA GGCTGGCTTC GAAGTTTCTG 
AAATCTGATC ATTTGTTTGT CGTGGTCGGA TTAGTTGGAG GAGCTTGTAG TGATGTGGAA 
CAAACAATCC TTGAAATACA AGAATATCGA AAAAGGGACA AGCTGGTTGA AATTCTGCAA 
AGCTCAGGTA ATGAGCGCAC AATGATTTTT GTAAATACAA AAAAGAAAGC AGATGTCATT 
GCTGGTTACC TTTGTCAAGA ACATTTTCCG ACAACAAGCA TTCACGGTGA TAGAGAACAA 
TGTCAAAGAG AGGAAGCTAT CCGGGATTTC AGAAGTGGGA AGTGTCCTGT TATTGTCTGT 
ACAGCAGTTG CTGCGAGAGG TTTAGATATT GAAAATGTTC AACATGTGAT AAATTATGAC 
GTTCCTAAGG AAATCGACGA GTATGTCCAT AGAATTGGTC GCACTGGTCG CTGTGGTAAC 
GTTGGAAAGG CAACTTCATT TTTTAATGTT AATGAAGACC ATGTTGTTGC TCGTCCCCTT 
GTGAAAATCC TTACTGATGC TCATCAAGAA GTCCCTGCTT GGTTAGAAGA AATTGCCTTT 
GGTGGCCATG GAGCTTTGAA CAGCTTATAT GCAGCAGACT CTATGGGTGG AGAGGCTGGA 
GAAAAGTACG TCAGTGCTCC TTCATCTGCA CAAGAGGAGG AGGCCAGCTG GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx4 ( XM_012962855.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818309.1 Xenopus tropicalis DEAD-box helicase 4 (ddx4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa47909
Clone ID Related Accession (Same CDS sequence)	XM_012962855.3
Accession Version	XM_012962855.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962855.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA ACTGGGATAC AGAAATTTCA ACTGAAAAAC CAACTTACAT CCCGAATTTC 
AGTTCCTTGG AGACAGACAA TACAGACAAT TACACTGCCT ACTCCAACAA TGATATCACC 
AATCAAAATT ATCAATCTGA AAGAAGTTTT GGAAATCGAG GAGGTTATCG ATCTGAAAGG 
GGTGGGCCTA CTAATTTTAA CCGAGGAAGT CGAATAGGAA GGGGAAGAGG GTTTGATAGT 
AATCGAAATG ACCAATATTC AGATTCTTCT GAGAGAGATG GATATAATGC TAACGAGGAT 
ACACAGAAAC CTTTTCGAGG AAGAGGTGGG TTCAGAAATG AGAATGGACA GCGGCGAGGT 
TTTGGAGACA GAGGAGGATT TAGAAATGAA AATGGACAGC AAAGAAACTT CGATAGAGGA 
GATTGGAATA ATTCTGGTGA AGGAGAGGAG CGTCCTCGTG GTTTTGGGAG AGGTGGTTTT 
AACAACTTTG ACTCTGATAC TGGTGGAAGA GGACATAGAG GCGGCCGTGG TGGCAGGGGT 
GGATACAAAG GAAGAAATGA AGAAGTTGGT GTTGAATCTG GAAAAAATCA AGAAGAGGGA 
ACAGAAAAAG AAGTAAAAAA GGTGATCTAT GTTCCCCTAC CGCCATCTGA TAGTGAAGAT 
GATATATTCC GGCACTACCA GTCTGGAATC AATTTTGATA AATATGATGA GATTCTTGTT 
GATGTGACAG GAAAGGATGT TCCTCCAGCC ATATTGACTT TTGAAGAAGC TAACTTTTGT 
GAGACACTAA GCAGGAATGT CACTAAAGCT GGATATGTAA AACTAACACC AGTGCAGAAA 
CACAGTATAC CTATTATATT GGCTGGTCGT GATTTAATGG CTTGTGCCCA GACCGGTTCT 
GGTAAAACTG CTGCTTTTCT GTTGCCAATC CTAAGTCATA TGATGAATGA AGGCATTACA 
GCTAGCCAGT TTTTACCACT TCAGGAGCCG CAAGCCATAA TTATTGCCCC TACCAGAGAA 
CTTATTAACC AGATATATCT CGATGCCCGA AAATTTTCAT ATGGAACATG TGTTCGTCCA 
GTAGTTGTAT ATGGAGGTAT ACATCCTGTG CATGCAATGA GGGATGTTGA AAGAGGGTGC 
AATATACTTT GTGCAACCCC TGGAAGATTG ATGGACATAA TAGGTAGAGA AAAGATTGGC 
TTAAGTAAGC TAAAATATCT AGTTCTGGAT GAAGCGGATC GCATGTTGGA TATGGGGTTT 
GCCCCAGTAA TGGAGAACTT AATAGGAAGT CCAGGAATGC CAGCAAAAGA GGAGCGACAA 
ACACTGATGT TCAGTGCTAC CTATCCTGCT GAAATCCAGA GGCTGGCTTC GAAGTTTCTG 
AAATCTGATC ATTTGTTTGT CGTGGTCGGA TTAGTTGGAG GAGCTTGTAG TGATGTGGAA 
CAAACAATCC TTGAAATACA AGAATATCGA AAAAGGGACA AGCTGGTTGA AATTCTGCAA 
AGCTCAGGTA ATGAGCGCAC AATGATTTTT GTAAATACAA AAAAGAAAGC AGATGTCATT 
GCTGGTTACC TTTGTCAAGA ACATTTTCCG ACAACAAGCA TTCACGGTGA TAGAGAACAA 
TGTCAAAGAG AGGAAGCTAT CCGGGATTTC AGAAGTGGGA AGTGTCCTGT TATTGTCTGT 
ACAGCAGTTG CTGCGAGAGG CTTAGATATT GAAAATGTTC AACATGTGAT AAATTATGAC 
GTTCCTAAGG AAATCGACGA GTATGTCCAT AGAATTGGTC GCACTGGTCG CTGTGGTAAC 
GTTGGAAAGG CAACTTCATT TTTTAATGTT AATGAAGACC ATGTTGTTGC TCGTCCCCTT 
GTGAAAATCC TTACTGATGC TCATCAAGAA GTCCCTGCTT GGTTAGAAGA AATTGCCTTT 
GGTGGCCATG GAGCTTTGAA CAGCTTATAT GCAGCAGACT CTATGGGTGG AGAGGCTGGA 
GAAAAGTACG TCAGTGCTCC TTCATCTGCA CAAGAGGAGG AGGCCAGCTG GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818309.1
CDS	173..2209
Translation	MEENWDTEISTEKPTYIPNFSSLETDNTDNYTAYSNNDITNQNYQSERSFGNRGGYRSERGGPTNFNRGSRIGRGRGFDSNRNDQYSDSSERDGYNANEDTQKPFRGRGGFRNENGQRRGFGDRGGFRNENGQQRNFDRGDWNNSGEGEERPRGFGRGGFNNFDSDTGGRGHRGGRGGRGGYKGRNEEVGVESGKNQEEGTEKEVKKVIYVPLPPSDSEDDIFRHYQSGINFDKYDEILVDVTGKDVPPAILTFEEANFCETLSRNVTKAGYVKLTPVQKHSIPIILAGRDLMACAQTGSGKTAAFLLPILSHMMNEGITASQFLPLQEPQAIIIAPTRELINQIYLDARKFSYGTCVRPVVVYGGIHPVHAMRDVERGCNILCATPGRLMDIIGREKIGLSKLKYLVLDEADRMLDMGFAPVMENLIGSPGMPAKEERQTLMFSATYPAEIQRLASKFLKSDHLFVVVGLVGGACSDVEQTILEIQEYRKRDKLVEILQSSGNERTMIFVNTKKKADVIAGYLCQEHFPTTSIHGDREQCQREEAIRDFRSGKCPVIVCTAVAARGLDIENVQHVINYDVPKEIDEYVHRIGRTGRCGNVGKATSFFNVNEDHVVARPLVKILTDAHQEVPAWLEEIAFGGHGALNSLYAADSMGGEAGEKYVSAPSSAQEEEASWD

Target ORF information:

RefSeq Version	XM_012962855.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAD-box helicase 4 (ddx4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962855.3

ORF Insert Sequence:

ATGGAGGAAA ACTGGGATAC AGAAATTTCA ACTGAAAAAC CAACTTACAT CCCGAATTTC 
AGTTCCTTGG AGACAGACAA TACAGACAAT TACACTGCCT ACTCCAACAA TGATATCACC 
AATCAAAATT ATCAATCTGA AAGAAGTTTT GGAAATCGAG GAGGTTATCG ATCTGAAAGG 
GGTGGGCCTA CTAATTTTAA CCGAGGAAGT CGAATAGGAA GGGGAAGAGG GTTTGATAGT 
AATCGAAATG ACCAATATTC AGATTCTTCT GAGAGAGATG GATATAATGC TAACGAGGAT 
ACACAGAAAC CTTTTCGAGG AAGAGGTGGG TTCAGAAATG AGAATGGACA GCGGCGAGGT 
TTTGGAGACA GAGGAGGATT TAGAAATGAA AATGGACAGC AAAGAAACTT CGATAGAGGA 
GATTGGAATA ATTCTGGTGA AGGAGAGGAG CGTCCTCGTG GTTTTGGGAG AGGTGGTTTT 
AACAACTTTG ACTCTGATAC TGGTGGAAGA GGACATAGAG GCGGCCGTGG TGGCAGGGGT 
GGATACAAAG GAAGAAATGA AGAAGTTGGT GTTGAATCTG GAAAAAATCA AGAAGAGGGA 
ACAGAAAAAG AAGTAAAAAA GGTGATCTAT GTTCCCCTAC CGCCATCTGA TAGTGAAGAT 
GATATATTCC GGCACTACCA GTCTGGAATC AATTTTGATA AATATGATGA GATTCTTGTT 
GATGTGACAG GAAAGGATGT TCCTCCAGCC ATATTGACTT TTGAAGAAGC TAACTTTTGT 
GAGACACTAA GCAGGAATGT CACTAAAGCT GGATATGTAA AACTAACACC AGTGCAGAAA 
CACAGTATAC CTATTATATT GGCTGGTCGT GATTTAATGG CTTGTGCCCA GACCGGTTCT 
GGTAAAACTG CTGCTTTTCT GTTGCCAATC CTAAGTCATA TGATGAATGA AGGCATTACA 
GCTAGCCAGT TTTTACCACT TCAGGAGCCG CAAGCCATAA TTATTGCCCC TACCAGAGAA 
CTTATTAACC AGATATATCT CGATGCCCGA AAATTTTCAT ATGGAACATG TGTTCGTCCA 
GTAGTTGTAT ATGGAGGTAT ACATCCTGTG CATGCAATGA GGGATGTTGA AAGAGGGTGC 
AATATACTTT GTGCAACCCC TGGAAGATTG ATGGACATAA TAGGTAGAGA AAAGATTGGC 
TTAAGTAAGC TAAAATATCT AGTTCTGGAT GAAGCGGATC GCATGTTGGA TATGGGGTTT 
GCCCCAGTAA TGGAGAACTT AATAGGAAGT CCAGGAATGC CAGCAAAAGA GGAGCGACAA 
ACACTGATGT TCAGTGCTAC CTATCCTGCT GAAATCCAGA GGCTGGCTTC GAAGTTTCTG 
AAATCTGATC ATTTGTTTGT CGTGGTCGGA TTAGTTGGAG GAGCTTGTAG TGATGTGGAA 
CAAACAATCC TTGAAATACA AGAATATCGA AAAAGGGACA AGCTGGTTGA AATTCTGCAA 
AGCTCAGGTA ATGAGCGCAC AATGATTTTT GTAAATACAA AAAAGAAAGC AGATGTCATT 
GCTGGTTACC TTTGTCAAGA ACATTTTCCG ACAACAAGCA TTCACGGTGA TAGAGAACAA 
TGTCAAAGAG AGGAAGCTAT CCGGGATTTC AGAAGTGGGA AGTGTCCTGT TATTGTCTGT 
ACAGCAGTTG CTGCGAGAGG CTTAGATATT GAAAATGTTC AACATGTGAT AAATTATGAC 
GTTCCTAAGG AAATCGACGA GTATGTCCAT AGAATTGGTC GCACTGGTCG CTGTGGTAAC 
GTTGGAAAGG CAACTTCATT TTTTAATGTT AATGAAGACC ATGTTGTTGC TCGTCCCCTT 
GTGAAAATCC TTACTGATGC TCATCAAGAA GTCCCTGCTT GGTTAGAAGA AATTGCCTTT 
GGTGGCCATG GAGCTTTGAA CAGCTTATAT GCAGCAGACT CTATGGGTGG AGAGGCTGGA 
GAAAAGTACG TCAGTGCTCC TTCATCTGCA CAAGAGGAGG AGGCCAGCTG GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx4 ( XM_012962857.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818311.1 Xenopus tropicalis DEAD-box helicase 4 (ddx4), transcript variant X2, mRNA.

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CloneID	OXa47910
Clone ID Related Accession (Same CDS sequence)	XM_012962857.3
Accession Version	XM_012962857.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962857.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA ACTGGGATAC AGAAATTTCA ACTGAAAAAC CAACTTACAT CCCGAATTTC 
AGTTCCTTGG AGACAGACAA TACAGACAAT TACACTGCCT ACTCCAACAA TGATATCACC 
AATCAAAATT ATCAATCTGA AAGAAGTTTT GGAAATCGAG GAGGTTATCG ATCTGAAAGG 
GGTGGGCCTA CTAATTTTAA CCGAGGAAGT CGAATAGGAA GGGGAAGAGG GTTTGATAGT 
AATCGAAATG ACCAATATTC AGATTCTTCT GAGAGAGATG GATATAATGC TAACGAGGAT 
ACACAGAAAC CTTTTCGAGG AAGAGGTGGA TTTAGAAATG AAAATGGACA GCAAAGAAAC 
TTCGATAGAG GAGATTGGAA TAATTCTGGT GAAGGAGAGG AGCGTCCTCG TGGTTTTGGG 
AGAGGTGGTT TTAACAACTT TGACTCTGAT ACTGGTGGAA GAGGACATAG AGGCGGCCGT 
GGTGGCAGGG GTGGATACAA AGGAAGAAAT GAAGAAGTTG GTGTTGAATC TGGAAAAAAT 
CAAGAAGAGG GAACAGAAAA AGAAGTAAAA AAGGTGATCT ATGTTCCCCT ACCGCCATCT 
GATAGTGAAG ATGATATATT CCGGCACTAC CAGTCTGGAA TCAATTTTGA TAAATATGAT 
GAGATTCTTG TTGATGTGAC AGGAAAGGAT GTTCCTCCAG CCATATTGAC TTTTGAAGAA 
GCTAACTTTT GTGAGACACT AAGCAGGAAT GTCACTAAAG CTGGATATGT AAAACTAACA 
CCAGTGCAGA AACACAGTAT ACCTATTATA TTGGCTGGTC GTGATTTAAT GGCTTGTGCC 
CAGACCGGTT CTGGTAAAAC TGCTGCTTTT CTGTTGCCAA TCCTAAGTCA TATGATGAAT 
GAAGGCATTA CAGCTAGCCA GTTTTTACCA CTTCAGGAGC CGCAAGCCAT AATTATTGCC 
CCTACCAGAG AACTTATTAA CCAGATATAT CTCGATGCCC GAAAATTTTC ATATGGAACA 
TGTGTTCGTC CAGTAGTTGT ATATGGAGGT ATACATCCTG TGCATGCAAT GAGGGATGTT 
GAAAGAGGGT GCAATATACT TTGTGCAACC CCTGGAAGAT TGATGGACAT AATAGGTAGA 
GAAAAGATTG GCTTAAGTAA GCTAAAATAT CTAGTTCTGG ATGAAGCGGA TCGCATGTTG 
GATATGGGGT TTGCCCCAGT AATGGAGAAC TTAATAGGAA GTCCAGGAAT GCCAGCAAAA 
GAGGAGCGAC AAACACTGAT GTTCAGTGCT ACCTATCCTG CTGAAATCCA GAGGCTGGCT 
TCGAAGTTTC TGAAATCTGA TCATTTGTTT GTCGTGGTCG GATTAGTTGG AGGAGCTTGT 
AGTGATGTGG AACAAACAAT CCTTGAAATA CAAGAATATC GAAAAAGGGA CAAGCTGGTT 
GAAATTCTGC AAAGCTCAGG TAATGAGCGC ACAATGATTT TTGTAAATAC AAAAAAGAAA 
GCAGATGTCA TTGCTGGTTA CCTTTGTCAA GAACATTTTC CGACAACAAG CATTCACGGT 
GATAGAGAAC AATGTCAAAG AGAGGAAGCT ATCCGGGATT TCAGAAGTGG GAAGTGTCCT 
GTTATTGTCT GTACAGCAGT TGCTGCGAGA GGCTTAGATA TTGAAAATGT TCAACATGTG 
ATAAATTATG ACGTTCCTAA GGAAATCGAC GAGTATGTCC ATAGAATTGG TCGCACTGGT 
CGCTGTGGTA ACGTTGGAAA GGCAACTTCA TTTTTTAATG TTAATGAAGA CCATGTTGTT 
GCTCGTCCCC TTGTGAAAAT CCTTACTGAT GCTCATCAAG AAGTCCCTGC TTGGTTAGAA 
GAAATTGCCT TTGGTGGCCA TGGAGCTTTG AACAGCTTAT ATGCAGCAGA CTCTATGGGT 
GGAGAGGCTG GAGAAAAGTA CGTCAGTGCT CCTTCATCTG CACAAGAGGA GGAGGCCAGC 
TGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818311.1
CDS	174..2162
Translation	MEENWDTEISTEKPTYIPNFSSLETDNTDNYTAYSNNDITNQNYQSERSFGNRGGYRSERGGPTNFNRGSRIGRGRGFDSNRNDQYSDSSERDGYNANEDTQKPFRGRGGFRNENGQQRNFDRGDWNNSGEGEERPRGFGRGGFNNFDSDTGGRGHRGGRGGRGGYKGRNEEVGVESGKNQEEGTEKEVKKVIYVPLPPSDSEDDIFRHYQSGINFDKYDEILVDVTGKDVPPAILTFEEANFCETLSRNVTKAGYVKLTPVQKHSIPIILAGRDLMACAQTGSGKTAAFLLPILSHMMNEGITASQFLPLQEPQAIIIAPTRELINQIYLDARKFSYGTCVRPVVVYGGIHPVHAMRDVERGCNILCATPGRLMDIIGREKIGLSKLKYLVLDEADRMLDMGFAPVMENLIGSPGMPAKEERQTLMFSATYPAEIQRLASKFLKSDHLFVVVGLVGGACSDVEQTILEIQEYRKRDKLVEILQSSGNERTMIFVNTKKKADVIAGYLCQEHFPTTSIHGDREQCQREEAIRDFRSGKCPVIVCTAVAARGLDIENVQHVINYDVPKEIDEYVHRIGRTGRCGNVGKATSFFNVNEDHVVARPLVKILTDAHQEVPAWLEEIAFGGHGALNSLYAADSMGGEAGEKYVSAPSSAQEEEASWD

Target ORF information:

RefSeq Version	XM_012962857.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAD-box helicase 4 (ddx4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962857.3

ORF Insert Sequence:

ATGGAGGAAA ACTGGGATAC AGAAATTTCA ACTGAAAAAC CAACTTACAT CCCGAATTTC 
AGTTCCTTGG AGACAGACAA TACAGACAAT TACACTGCCT ACTCCAACAA TGATATCACC 
AATCAAAATT ATCAATCTGA AAGAAGTTTT GGAAATCGAG GAGGTTATCG ATCTGAAAGG 
GGTGGGCCTA CTAATTTTAA CCGAGGAAGT CGAATAGGAA GGGGAAGAGG GTTTGATAGT 
AATCGAAATG ACCAATATTC AGATTCTTCT GAGAGAGATG GATATAATGC TAACGAGGAT 
ACACAGAAAC CTTTTCGAGG AAGAGGTGGA TTTAGAAATG AAAATGGACA GCAAAGAAAC 
TTCGATAGAG GAGATTGGAA TAATTCTGGT GAAGGAGAGG AGCGTCCTCG TGGTTTTGGG 
AGAGGTGGTT TTAACAACTT TGACTCTGAT ACTGGTGGAA GAGGACATAG AGGCGGCCGT 
GGTGGCAGGG GTGGATACAA AGGAAGAAAT GAAGAAGTTG GTGTTGAATC TGGAAAAAAT 
CAAGAAGAGG GAACAGAAAA AGAAGTAAAA AAGGTGATCT ATGTTCCCCT ACCGCCATCT 
GATAGTGAAG ATGATATATT CCGGCACTAC CAGTCTGGAA TCAATTTTGA TAAATATGAT 
GAGATTCTTG TTGATGTGAC AGGAAAGGAT GTTCCTCCAG CCATATTGAC TTTTGAAGAA 
GCTAACTTTT GTGAGACACT AAGCAGGAAT GTCACTAAAG CTGGATATGT AAAACTAACA 
CCAGTGCAGA AACACAGTAT ACCTATTATA TTGGCTGGTC GTGATTTAAT GGCTTGTGCC 
CAGACCGGTT CTGGTAAAAC TGCTGCTTTT CTGTTGCCAA TCCTAAGTCA TATGATGAAT 
GAAGGCATTA CAGCTAGCCA GTTTTTACCA CTTCAGGAGC CGCAAGCCAT AATTATTGCC 
CCTACCAGAG AACTTATTAA CCAGATATAT CTCGATGCCC GAAAATTTTC ATATGGAACA 
TGTGTTCGTC CAGTAGTTGT ATATGGAGGT ATACATCCTG TGCATGCAAT GAGGGATGTT 
GAAAGAGGGT GCAATATACT TTGTGCAACC CCTGGAAGAT TGATGGACAT AATAGGTAGA 
GAAAAGATTG GCTTAAGTAA GCTAAAATAT CTAGTTCTGG ATGAAGCGGA TCGCATGTTG 
GATATGGGGT TTGCCCCAGT AATGGAGAAC TTAATAGGAA GTCCAGGAAT GCCAGCAAAA 
GAGGAGCGAC AAACACTGAT GTTCAGTGCT ACCTATCCTG CTGAAATCCA GAGGCTGGCT 
TCGAAGTTTC TGAAATCTGA TCATTTGTTT GTCGTGGTCG GATTAGTTGG AGGAGCTTGT 
AGTGATGTGG AACAAACAAT CCTTGAAATA CAAGAATATC GAAAAAGGGA CAAGCTGGTT 
GAAATTCTGC AAAGCTCAGG TAATGAGCGC ACAATGATTT TTGTAAATAC AAAAAAGAAA 
GCAGATGTCA TTGCTGGTTA CCTTTGTCAA GAACATTTTC CGACAACAAG CATTCACGGT 
GATAGAGAAC AATGTCAAAG AGAGGAAGCT ATCCGGGATT TCAGAAGTGG GAAGTGTCCT 
GTTATTGTCT GTACAGCAGT TGCTGCGAGA GGCTTAGATA TTGAAAATGT TCAACATGTG 
ATAAATTATG ACGTTCCTAA GGAAATCGAC GAGTATGTCC ATAGAATTGG TCGCACTGGT 
CGCTGTGGTA ACGTTGGAAA GGCAACTTCA TTTTTTAATG TTAATGAAGA CCATGTTGTT 
GCTCGTCCCC TTGTGAAAAT CCTTACTGAT GCTCATCAAG AAGTCCCTGC TTGGTTAGAA 
GAAATTGCCT TTGGTGGCCA TGGAGCTTTG AACAGCTTAT ATGCAGCAGA CTCTATGGGT 
GGAGAGGCTG GAGAAAAGTA CGTCAGTGCT CCTTCATCTG CACAAGAGGA GGAGGCCAGC 
TGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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ddx4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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