ORF » Species Summary » Xenopus tropicalis » spast cDNA ORF clone

spast cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following spast gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spast cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa00683 NM_001016453.2
Latest version!		 Xenopus tropicalis spastin (spast), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OXa27740 XM_012963010.3
Latest version!		 Xenopus tropicalis spastin (spast), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OXa27740 XM_012963010.2 Xenopus tropicalis spastin (spast), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    spast ( NM_001016453.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001016453.1 Xenopus tropicalis spastin (spast), mRNA.
    Price & Availability↑
    CloneID OXa00683
    Clone ID Related Accession (Same CDS sequence) NM_001016453.2
    Accession Version NM_001016453.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1716bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spastin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR760974.2. On Oct 14, 2005 this sequence version replaced NM_001016453.1. ##Evidence-Data-START## Transcript exon combination :: CR760974.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAATTCTC CGGGCGGAAG AAACGACAAG AAGAAACCGG TGACCCCAGC GGCGGAGACT 
    GGTCCCGGCC CGCCGACGCC TCCTCCTCCT CCAGCAGAGA CGCAGGTCCT GCTCGCCCCG 
    CCCTCACTGC ATAAGCGGAA CCTTTACCTG TTCTCCTACC CCCTGCTCGC CGCTTTCTCC 
    CTGCTACGCT TCCTGGCTTT TCAGCTGGGG CTGCTGTTCG TTTGGTTTTG TGAGCGGCTT 
    TCCCGTAGGG TCATGGCGGA CAAAGGGAGT ACGGCGGCCC GCACAGCAGC GGCTCCAGCT 
    CAGGACAGGC CCCAGGAGCC TGAGGTAGTG AGGAGCTATC ACCAGCAGGC CTTCCAGTAC 
    ATCTCTATGG CGCTGCGCAT CGACGAGGAG GAGAAAGATC AAAAGGAGCA AGCCATTCAG 
    TGGTACAAGA AGGGTATTGA GGAACTGGAG AAAGGAATAG CTGTAACTAT AACTGGCAAA 
    GGTGAACAAT ATGACAGAGC CCGTCGTTTA CAAGCTAAAA TGTCAACAAA CTTGTTAATG 
    GCAAAGGATC GCTTACAGCT ACTAGAAAAA GGGGCTGTTC CCAAAAAAAA AGATCCACCA 
    TCGATTTCAA GTAACTCTTA TTCTCGTGTA AAGGCTGCTC CTAAGAGCGG GTCATTGGGC 
    AACAGAATAC CTAACTGTAC TGGTGTATCA TCTTCAGCTA GGCAAGCTGG TCCAAATGCA 
    CCTTCTAACA GGGGAGCGGC AGGAAAGAAC AACACAAGAA CAAATAAACC AACAACACCC 
    ACTACTGCAG TACGGAAAAA AGACATGAAA AATTTAAGAA ATGTAGATAG TAATCTTGCA 
    AACTTGATTT TGAATGAAAT TGTGGATAGT GGTCCTACTG TCAAGTTTGC TGACATTGCT 
    GGACAAGATC TAGCCAAGCA AGCCTTGCAG GAGATAGTTA TTCTTCCTTC TATACGACCC 
    GAGTTATTTA CTGGGCTAAG AGCGCCTGCA CGAGGATTGC TGCTGTTTGG TCCTCCAGGG 
    AATGGAAAGA CTATGCTGGC AAAAGCTGTT GCTGCTGAAT CAAATGCAAC ATTCTTCAAC 
    ATAAGTGCAG CGAGTCTGAC TTCAAAATAT GTGGGAGAAG GTGAAAAGCT GGTGCGTGCA 
    CTCTTTTCTG TGGCTAGAGA ATTGCAACCT TCCATTATAT TTATAGATGA GGTTGATAGT 
    CTTTTATGTG AAAGAAGAGA AGGGGAGCAT GATGCAAGCA GAAGACTGAA AACTGAATTT 
    CTAATAGAGT TTGATGGTGT ACAATCTGGG GGAGATGACA GAGTTCTTGT TATGGGAGCA 
    ACTAACAGAC CACAGGAACT TGATGATGCA GTTCTCAGGA GATTCACCAA GAGGGTTTAT 
    GTATCTTTAC CAAATGAAGA GACTAGACTC TTACTTCTCA AAAATCTTCT GAGTAAACAA 
    GGTAACCCAC TTAATGAGAA GGAACTGACT CAGTTATCTA GGCTGACAGA AGGATACTCT 
    GGGAGTGATA TAACAGCTTT GGCAAAAGAT GCTGCTTTGG GACCAATCAG AGAGTTAAAA 
    CCTGAACAAG TCAAAAATAT GGCTGCTAGT GAGATGAGAA ATATAAAATA TTCAGATTTT 
    CTAAGTTCAT TAAAGAAGAT AAAGTGCAGT GTGAGCCCTT CAACCTTGGA ATCTTATATA 
    AGGTGGAATA AGGAATTTGG CGATACTACA GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001016453.1
    CDS50..1765
    Translation

    Target ORF information:

    RefSeq Version NM_001016453.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spastin (spast), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001016453.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAATTCTC CGGGCGGAAG AAACGACAAG AAGAAACCGG TGACCCCAGC GGCGGAGACT 
    GGTCCCGGCC CGCCGACGCC TCCTCCTCCT CCAGCAGAGA CGCAGGTCCT GCTCGCCCCG 
    CCCTCACTGC ATAAGCGGAA CCTTTACCTG TTCTCCTACC CCCTGCTCGC CGCTTTCTCC 
    CTGCTACGCT TCCTGGCTTT TCAGCTGGGG CTGCTGTTCG TTTGGTTTTG TGAGCGGCTT 
    TCCCGTAGGG TCATGGCGGA CAAAGGGAGT ACGGCGGCCC GCACAGCAGC GGCTCCAGCT 
    CAGGACAGGC CCCAGGAGCC TGAGGTAGTG AGGAGCTATC ACCAGCAGGC CTTCCAGTAC 
    ATCTCTATGG CGCTGCGCAT CGACGAGGAG GAGAAAGATC AAAAGGAGCA AGCCATTCAG 
    TGGTACAAGA AGGGTATTGA GGAACTGGAG AAAGGAATAG CTGTAACTAT AACTGGCAAA 
    GGTGAACAAT ATGACAGAGC CCGTCGTTTA CAAGCTAAAA TGTCAACAAA CTTGTTAATG 
    GCAAAGGATC GCTTACAGCT ACTAGAAAAA GGGGCTGTTC CCAAAAAAAA AGATCCACCA 
    TCGATTTCAA GTAACTCTTA TTCTCGTGTA AAGGCTGCTC CTAAGAGCGG GTCATTGGGC 
    AACAGAATAC CTAACTGTAC TGGTGTATCA TCTTCAGCTA GGCAAGCTGG TCCAAATGCA 
    CCTTCTAACA GGGGAGCGGC AGGAAAGAAC AACACAAGAA CAAATAAACC AACAACACCC 
    ACTACTGCAG TACGGAAAAA AGACATGAAA AATTTAAGAA ATGTAGATAG TAATCTTGCA 
    AACTTGATTT TGAATGAAAT TGTGGATAGT GGTCCTACTG TCAAGTTTGC TGACATTGCT 
    GGACAAGATC TAGCCAAGCA AGCCTTGCAG GAGATAGTTA TTCTTCCTTC TATACGACCC 
    GAGTTATTTA CTGGGCTAAG AGCGCCTGCA CGAGGATTGC TGCTGTTTGG TCCTCCAGGG 
    AATGGAAAGA CTATGCTGGC AAAAGCTGTT GCTGCTGAAT CAAATGCAAC ATTCTTCAAC 
    ATAAGTGCAG CGAGTCTGAC TTCAAAATAT GTGGGAGAAG GTGAAAAGCT GGTGCGTGCA 
    CTCTTTTCTG TGGCTAGAGA ATTGCAACCT TCCATTATAT TTATAGATGA GGTTGATAGT 
    CTTTTATGTG AAAGAAGAGA AGGGGAGCAT GATGCAAGCA GAAGACTGAA AACTGAATTT 
    CTAATAGAGT TTGATGGTGT ACAATCTGGG GGAGATGACA GAGTTCTTGT TATGGGAGCA 
    ACTAACAGAC CACAGGAACT TGATGATGCA GTTCTCAGGA GATTCACCAA GAGGGTTTAT 
    GTATCTTTAC CAAATGAAGA GACTAGACTC TTACTTCTCA AAAATCTTCT GAGTAAACAA 
    GGTAACCCAC TTAATGAGAA GGAACTGACT CAGTTATCTA GGCTGACAGA AGGATACTCT 
    GGGAGTGATA TAACAGCTTT GGCAAAAGAT GCTGCTTTGG GACCAATCAG AGAGTTAAAA 
    CCTGAACAAG TCAAAAATAT GGCTGCTAGT GAGATGAGAA ATATAAAATA TTCAGATTTT 
    CTAAGTTCAT TAAAGAAGAT AAAGTGCAGT GTGAGCCCTT CAACCTTGGA ATCTTATATA 
    AGGTGGAATA AGGAATTTGG CGATACTACA GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    spast ( XM_012963010.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818464.1 Xenopus tropicalis spastin (spast), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OXa27740
    Clone ID Related Accession (Same CDS sequence) XM_012963010.3 , XM_012963010.2
    Accession Version XM_012963010.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1812bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spastin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAATTCTC CGGGCGGAAG AAACAACAAG AAGAAACCGG TGACCCCAGC GGCGGAGACT 
    GGTCCCGGCC CGCCGACGCC TCCTCCTCCT CCAGCAGAGA CGCAGGTCCT GCTCGCCCCG 
    CCCTCACTGC ATAAGCGGAA CCTTTACCTG TTCTCCTATC CCCTGCTCGC CGCTTTCTCC 
    CTGCTACGCT TCCTGGCTTT TCAGCTGGGG CTGCTGTTCG TTTGGTTTTG TGAGCGGCTT 
    TCCCGTAGGG TCATGGCGGA CAAAGGGAGT ACGGCGGCCC GCACAGCAGC GGCTCCAGCT 
    CAGGACAGGC CCCAGGAGCC TGAGGTAGTG AGGAGCTATC ACCAGCAGGC CTTCCAGTAC 
    ATCTCTATGG CGCTGCGCAT CGACGAGGAG GAGAAAGATC AAAAGGAGCA AGCCATTCAG 
    TGGTACAAGA AGGGTATTGA GGAACTGGAG AAAGGAATAG CTGTAACTAT AACTGGCAAA 
    GGTGAACAAT ATGACAGAGC CCGTCGTTTA CAAGCTAAAA TGTCAACAAA CTTGTTAATG 
    GCAAAGGATC GCTTACAGCT ACTAGCAAAG CTGAAAGCAG ATATACAAGG TCAGCACTCG 
    CAAATGGAGG TCTGTAGTGA CAATACTAAC TTACCATGCC GCAATGGACT TCTCAAGCCA 
    GAAAAAGGGG CTGTTCCCAA AAAAAAAGAT CCACCATCGA TTTCAAGTAA CTCTTATTCT 
    CGTGTAAAGG CTGCTCCTAA GAGCGGGTCA TTGGGCAACA GAATACCTAA CTGTACTGGT 
    GTATCATCTT CAGCTAGGCA AGCTGGTCCA AATGCACCTT CTAACAGGGG AGCGGCAGGA 
    AAGAACAACA CAAGAACAAA TAAACCAACA ACACCCACTA CTGCAGTACG GAAAAAAGAC 
    ATGAAAAATT TAAGAAATGT AGATAGTAAT CTTGCAAACT TGATTTTGAA TGAAATTGTG 
    GATAGTGGTC CTACTGTCAA GTTTGCTGAC ATTGCTGGAC AAGATCTAGC CAAGCAAGCC 
    TTGCAGGAGA TAGTTATTCT TCCTTCTATA CGACCCGAGT TATTTACTGG GCTAAGAGCG 
    CCTGCACGAG GATTGCTGCT GTTTGGTCCT CCAGGGAATG GAAAGACTAT GCTGGCAAAA 
    GCTGTTGCTG CTGAATCAAA TGCAACATTC TTCAACATAA GTGCAGCGAG TCTGACTTCA 
    AAATATGTGG GAGAAGGTGA AAAGCTGGTG CGTGCACTCT TTTCTGTGGC TAGAGAATTG 
    CAACCTTCCA TTATATTTAT AGATGAGGTT GATAGCCTTT TATGTGAAAG AAGAGAAGGG 
    GAGCATGATG CAAGCAGAAG ACTGAAAACT GAATTTCTAA TAGAGTTTGA TGGTGTACAA 
    TCTGGGGGAG ATGACAGAGT TCTTGTTATG GGAGCAACTA ACAGACCACA GGAACTTGAT 
    GATGCAGTTC TCAGGAGATT CACCAAGAGG GTTTATGTAT CTTTACCAAA TGAAGAGACT 
    AGACTCTTAC TTCTCAAAAA TCTTCTGAGT AAACAAGGTA ACCCACTTAA TGAGAAGGAA 
    CTGACTCAGT TATCTAGGCT GACAGAAGGA TACTCTGGGA GTGATATAAC AGCTTTGGCA 
    AAAGATGCTG CTTTGGGACC AATCAGAGAG TTAAAACCTG AACAAGTCAA AAATATGGCT 
    GCTAGTGAGA TGAGAAATAT AAAATATTCA GATTTTCTAA GTTCATTAAA GAAGATAAAG 
    TGCAGTGTGA GCCCTTCAAC CTTGGAATCT TATATAAGGT GGAATAAGGA ATTTGGCGAT 
    ACTACAGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818464.1
    CDS94..1905
    Translation

    Target ORF information:

    RefSeq Version XM_012963010.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spastin (spast), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012963010.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAATTCTC CGGGCGGAAG AAACAACAAG AAGAAACCGG TGACCCCAGC GGCGGAGACT 
    GGTCCCGGCC CGCCGACGCC TCCTCCTCCT CCAGCAGAGA CGCAGGTCCT GCTCGCCCCG 
    CCCTCACTGC ATAAGCGGAA CCTTTACCTG TTCTCCTATC CCCTGCTCGC CGCTTTCTCC 
    CTGCTACGCT TCCTGGCTTT TCAGCTGGGG CTGCTGTTCG TTTGGTTTTG TGAGCGGCTT 
    TCCCGTAGGG TCATGGCGGA CAAAGGGAGT ACGGCGGCCC GCACAGCAGC GGCTCCAGCT 
    CAGGACAGGC CCCAGGAGCC TGAGGTAGTG AGGAGCTATC ACCAGCAGGC CTTCCAGTAC 
    ATCTCTATGG CGCTGCGCAT CGACGAGGAG GAGAAAGATC AAAAGGAGCA AGCCATTCAG 
    TGGTACAAGA AGGGTATTGA GGAACTGGAG AAAGGAATAG CTGTAACTAT AACTGGCAAA 
    GGTGAACAAT ATGACAGAGC CCGTCGTTTA CAAGCTAAAA TGTCAACAAA CTTGTTAATG 
    GCAAAGGATC GCTTACAGCT ACTAGCAAAG CTGAAAGCAG ATATACAAGG TCAGCACTCG 
    CAAATGGAGG TCTGTAGTGA CAATACTAAC TTACCATGCC GCAATGGACT TCTCAAGCCA 
    GAAAAAGGGG CTGTTCCCAA AAAAAAAGAT CCACCATCGA TTTCAAGTAA CTCTTATTCT 
    CGTGTAAAGG CTGCTCCTAA GAGCGGGTCA TTGGGCAACA GAATACCTAA CTGTACTGGT 
    GTATCATCTT CAGCTAGGCA AGCTGGTCCA AATGCACCTT CTAACAGGGG AGCGGCAGGA 
    AAGAACAACA CAAGAACAAA TAAACCAACA ACACCCACTA CTGCAGTACG GAAAAAAGAC 
    ATGAAAAATT TAAGAAATGT AGATAGTAAT CTTGCAAACT TGATTTTGAA TGAAATTGTG 
    GATAGTGGTC CTACTGTCAA GTTTGCTGAC ATTGCTGGAC AAGATCTAGC CAAGCAAGCC 
    TTGCAGGAGA TAGTTATTCT TCCTTCTATA CGACCCGAGT TATTTACTGG GCTAAGAGCG 
    CCTGCACGAG GATTGCTGCT GTTTGGTCCT CCAGGGAATG GAAAGACTAT GCTGGCAAAA 
    GCTGTTGCTG CTGAATCAAA TGCAACATTC TTCAACATAA GTGCAGCGAG TCTGACTTCA 
    AAATATGTGG GAGAAGGTGA AAAGCTGGTG CGTGCACTCT TTTCTGTGGC TAGAGAATTG 
    CAACCTTCCA TTATATTTAT AGATGAGGTT GATAGCCTTT TATGTGAAAG AAGAGAAGGG 
    GAGCATGATG CAAGCAGAAG ACTGAAAACT GAATTTCTAA TAGAGTTTGA TGGTGTACAA 
    TCTGGGGGAG ATGACAGAGT TCTTGTTATG GGAGCAACTA ACAGACCACA GGAACTTGAT 
    GATGCAGTTC TCAGGAGATT CACCAAGAGG GTTTATGTAT CTTTACCAAA TGAAGAGACT 
    AGACTCTTAC TTCTCAAAAA TCTTCTGAGT AAACAAGGTA ACCCACTTAA TGAGAAGGAA 
    CTGACTCAGT TATCTAGGCT GACAGAAGGA TACTCTGGGA GTGATATAAC AGCTTTGGCA 
    AAAGATGCTG CTTTGGGACC AATCAGAGAG TTAAAACCTG AACAAGTCAA AAATATGGCT 
    GCTAGTGAGA TGAGAAATAT AAAATATTCA GATTTTCTAA GTTCATTAAA GAAGATAAAG 
    TGCAGTGTGA GCCCTTCAAC CTTGGAATCT TATATAAGGT GGAATAAGGA ATTTGGCGAT 
    ACTACAGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    spast ( XM_012963010.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818464.1 Xenopus tropicalis spastin (spast), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OXa27740
    Clone ID Related Accession (Same CDS sequence) XM_012963010.3 , XM_012963010.2
    Accession Version XM_012963010.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1812bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spastin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963010.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAATTCTC CGGGCGGAAG AAACAACAAG AAGAAACCGG TGACCCCAGC GGCGGAGACT 
    GGTCCCGGCC CGCCGACGCC TCCTCCTCCT CCAGCAGAGA CGCAGGTCCT GCTCGCCCCG 
    CCCTCACTGC ATAAGCGGAA CCTTTACCTG TTCTCCTATC CCCTGCTCGC CGCTTTCTCC 
    CTGCTACGCT TCCTGGCTTT TCAGCTGGGG CTGCTGTTCG TTTGGTTTTG TGAGCGGCTT 
    TCCCGTAGGG TCATGGCGGA CAAAGGGAGT ACGGCGGCCC GCACAGCAGC GGCTCCAGCT 
    CAGGACAGGC CCCAGGAGCC TGAGGTAGTG AGGAGCTATC ACCAGCAGGC CTTCCAGTAC 
    ATCTCTATGG CGCTGCGCAT CGACGAGGAG GAGAAAGATC AAAAGGAGCA AGCCATTCAG 
    TGGTACAAGA AGGGTATTGA GGAACTGGAG AAAGGAATAG CTGTAACTAT AACTGGCAAA 
    GGTGAACAAT ATGACAGAGC CCGTCGTTTA CAAGCTAAAA TGTCAACAAA CTTGTTAATG 
    GCAAAGGATC GCTTACAGCT ACTAGCAAAG CTGAAAGCAG ATATACAAGG TCAGCACTCG 
    CAAATGGAGG TCTGTAGTGA CAATACTAAC TTACCATGCC GCAATGGACT TCTCAAGCCA 
    GAAAAAGGGG CTGTTCCCAA AAAAAAAGAT CCACCATCGA TTTCAAGTAA CTCTTATTCT 
    CGTGTAAAGG CTGCTCCTAA GAGCGGGTCA TTGGGCAACA GAATACCTAA CTGTACTGGT 
    GTATCATCTT CAGCTAGGCA AGCTGGTCCA AATGCACCTT CTAACAGGGG AGCGGCAGGA 
    AAGAACAACA CAAGAACAAA TAAACCAACA ACACCCACTA CTGCAGTACG GAAAAAAGAC 
    ATGAAAAATT TAAGAAATGT AGATAGTAAT CTTGCAAACT TGATTTTGAA TGAAATTGTG 
    GATAGTGGTC CTACTGTCAA GTTTGCTGAC ATTGCTGGAC AAGATCTAGC CAAGCAAGCC 
    TTGCAGGAGA TAGTTATTCT TCCTTCTATA CGACCCGAGT TATTTACTGG GCTAAGAGCG 
    CCTGCACGAG GATTGCTGCT GTTTGGTCCT CCAGGGAATG GAAAGACTAT GCTGGCAAAA 
    GCTGTTGCTG CTGAATCAAA TGCAACATTC TTCAACATAA GTGCAGCGAG TCTGACTTCA 
    AAATATGTGG GAGAAGGTGA AAAGCTGGTG CGTGCACTCT TTTCTGTGGC TAGAGAATTG 
    CAACCTTCCA TTATATTTAT AGATGAGGTT GATAGCCTTT TATGTGAAAG AAGAGAAGGG 
    GAGCATGATG CAAGCAGAAG ACTGAAAACT GAATTTCTAA TAGAGTTTGA TGGTGTACAA 
    TCTGGGGGAG ATGACAGAGT TCTTGTTATG GGAGCAACTA ACAGACCACA GGAACTTGAT 
    GATGCAGTTC TCAGGAGATT CACCAAGAGG GTTTATGTAT CTTTACCAAA TGAAGAGACT 
    AGACTCTTAC TTCTCAAAAA TCTTCTGAGT AAACAAGGTA ACCCACTTAA TGAGAAGGAA 
    CTGACTCAGT TATCTAGGCT GACAGAAGGA TACTCTGGGA GTGATATAAC AGCTTTGGCA 
    AAAGATGCTG CTTTGGGACC AATCAGAGAG TTAAAACCTG AACAAGTCAA AAATATGGCT 
    GCTAGTGAGA TGAGAAATAT AAAATATTCA GATTTTCTAA GTTCATTAAA GAAGATAAAG 
    TGCAGTGTGA GCCCTTCAAC CTTGGAATCT TATATAAGGT GGAATAAGGA ATTTGGCGAT 
    ACTACAGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818464.1
    CDS54..1865
    Translation

    Target ORF information:

    RefSeq Version XM_012963010.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spastin (spast), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012963010.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAATTCTC CGGGCGGAAG AAACAACAAG AAGAAACCGG TGACCCCAGC GGCGGAGACT 
    GGTCCCGGCC CGCCGACGCC TCCTCCTCCT CCAGCAGAGA CGCAGGTCCT GCTCGCCCCG 
    CCCTCACTGC ATAAGCGGAA CCTTTACCTG TTCTCCTATC CCCTGCTCGC CGCTTTCTCC 
    CTGCTACGCT TCCTGGCTTT TCAGCTGGGG CTGCTGTTCG TTTGGTTTTG TGAGCGGCTT 
    TCCCGTAGGG TCATGGCGGA CAAAGGGAGT ACGGCGGCCC GCACAGCAGC GGCTCCAGCT 
    CAGGACAGGC CCCAGGAGCC TGAGGTAGTG AGGAGCTATC ACCAGCAGGC CTTCCAGTAC 
    ATCTCTATGG CGCTGCGCAT CGACGAGGAG GAGAAAGATC AAAAGGAGCA AGCCATTCAG 
    TGGTACAAGA AGGGTATTGA GGAACTGGAG AAAGGAATAG CTGTAACTAT AACTGGCAAA 
    GGTGAACAAT ATGACAGAGC CCGTCGTTTA CAAGCTAAAA TGTCAACAAA CTTGTTAATG 
    GCAAAGGATC GCTTACAGCT ACTAGCAAAG CTGAAAGCAG ATATACAAGG TCAGCACTCG 
    CAAATGGAGG TCTGTAGTGA CAATACTAAC TTACCATGCC GCAATGGACT TCTCAAGCCA 
    GAAAAAGGGG CTGTTCCCAA AAAAAAAGAT CCACCATCGA TTTCAAGTAA CTCTTATTCT 
    CGTGTAAAGG CTGCTCCTAA GAGCGGGTCA TTGGGCAACA GAATACCTAA CTGTACTGGT 
    GTATCATCTT CAGCTAGGCA AGCTGGTCCA AATGCACCTT CTAACAGGGG AGCGGCAGGA 
    AAGAACAACA CAAGAACAAA TAAACCAACA ACACCCACTA CTGCAGTACG GAAAAAAGAC 
    ATGAAAAATT TAAGAAATGT AGATAGTAAT CTTGCAAACT TGATTTTGAA TGAAATTGTG 
    GATAGTGGTC CTACTGTCAA GTTTGCTGAC ATTGCTGGAC AAGATCTAGC CAAGCAAGCC 
    TTGCAGGAGA TAGTTATTCT TCCTTCTATA CGACCCGAGT TATTTACTGG GCTAAGAGCG 
    CCTGCACGAG GATTGCTGCT GTTTGGTCCT CCAGGGAATG GAAAGACTAT GCTGGCAAAA 
    GCTGTTGCTG CTGAATCAAA TGCAACATTC TTCAACATAA GTGCAGCGAG TCTGACTTCA 
    AAATATGTGG GAGAAGGTGA AAAGCTGGTG CGTGCACTCT TTTCTGTGGC TAGAGAATTG 
    CAACCTTCCA TTATATTTAT AGATGAGGTT GATAGCCTTT TATGTGAAAG AAGAGAAGGG 
    GAGCATGATG CAAGCAGAAG ACTGAAAACT GAATTTCTAA TAGAGTTTGA TGGTGTACAA 
    TCTGGGGGAG ATGACAGAGT TCTTGTTATG GGAGCAACTA ACAGACCACA GGAACTTGAT 
    GATGCAGTTC TCAGGAGATT CACCAAGAGG GTTTATGTAT CTTTACCAAA TGAAGAGACT 
    AGACTCTTAC TTCTCAAAAA TCTTCTGAGT AAACAAGGTA ACCCACTTAA TGAGAAGGAA 
    CTGACTCAGT TATCTAGGCT GACAGAAGGA TACTCTGGGA GTGATATAAC AGCTTTGGCA 
    AAAGATGCTG CTTTGGGACC AATCAGAGAG TTAAAACCTG AACAAGTCAA AAATATGGCT 
    GCTAGTGAGA TGAGAAATAT AAAATATTCA GATTTTCTAA GTTCATTAAA GAAGATAAAG 
    TGCAGTGTGA GCCCTTCAAC CTTGGAATCT TATATAAGGT GGAATAAGGA ATTTGGCGAT 
    ACTACAGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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