lig3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	lig3
Entrez Gene ID	549105
Full Name	ligase III, DNA, ATP-dependent
General protein information	Preferred Names ligase III, DNA, ATP-dependent Names DNA ligase 3
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012971765.2	XP_012827219.1	DNA ligase 3 isoform X1
XM_018097739.1	XP_017953228.1	DNA ligase 3 isoform X2
XM_012971764.2	XP_012827218.1	DNA ligase 3 isoform X1
XM_012971765.3	XP_012827219.2	DNA ligase 3 isoform X1
XM_018097738.1	XP_017953227.1	DNA ligase 3 isoform X1
NM_001016351.3	NP_001016351.2	DNA ligase 3
XM_012971764.3	XP_012827218.2	DNA ligase 3 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LIG3	NP_001033196.1
Rattus norvegicus (Norway rat)	Lig3	NP_001012011.1
Gallus gallus (chicken)	LIG3	NP_001006215.1
Homo sapiens (human)	LIG3	NP_039269.2
	LIG3	XP_001113780.1
Canis lupus familiaris (dog)	LIG3	XP_005624869.1
Mus musculus (house mouse)	Lig3	NP_034846.2
Drosophila melanogaster (fruit fly)	lig3	NP_650187.2
Xenopus tropicalis (tropical clawed frog)	lig3	NP_001016351.2
Pan troglodytes (chimpanzee)	LIG3	XP_511409.3
Danio rerio (zebrafish)	lig3	NP_001025345.2

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following lig3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lig3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa01518	NM_001016351.3 Latest version!	Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa27673	XM_018097738.1 Latest version!	Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa27673	XM_012971765.2 Latest version!	Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa27673	XM_012971764.2 Latest version!	Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa47019	XM_018097739.1 Latest version!	Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OXa50081	XM_012971765.3 Latest version!	Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa50081	XM_012971764.3 Latest version!	Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lig3 ( NM_001016351.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001016351.2 Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), mRNA.

Price & Availability↑

CloneID	OXa01518
Clone ID Related Accession (Same CDS sequence)	NM_001016351.3
Accession Version	NM_001016351.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA ligase 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC167946.1. On Dec 29, 2009 this sequence version replaced NM_001016351.2. ##Evidence-Data-START## Transcript exon combination :: BC167946.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT 
GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA 
TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT 
GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG 
GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA 
ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT 
GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT 
CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT 
ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA 
TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA 
AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG 
CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC 
GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG 
TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT 
AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC 
TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA 
GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC 
CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT 
AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT 
GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC 
AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC 
CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT 
CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT 
GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT 
CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA 
GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT 
CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC 
TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT 
AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT 
TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA 
AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT 
TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT 
GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA 
AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA 
CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA 
TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT 
CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG 
TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC 
ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC 
ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT 
GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCTCCT 
TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCTCC TCCTCAGAAG 
AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT 
AAAAAGGTTA GGAAACAACA TATAGAGAAA TGTATCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001016351.2
CDS	41..2722
Translation	MRAFLRLLAYQTKRTLGQSLTKVPLRVIFHACWSQSPLHSALHFQFFLRVTHFSNMAEQRYCVEYAKRGTAGCKKCKEKIGKGLVRIGKIVPNPFSESAGDMKEWYHIKCMFEKLERARATTKKIDDLTELEGWQELQDCDKNLISQHVTELATKTAATPRKKTPSKEKQSPAVQGTTSTTAPAASPSLKFSGFSAKPSQSSSPPSSSTGSSLSTAKCDPKHKDCLLREFRKLCAMVAEQPSYNSKTQIIRDFLTKGTSGDGFHGDTYLTVKLLLPGVIKSVYNLNDKQIVKHFSRIFNCNQEEMVRDLEQGDVSETVRIFFEESKCFAPASKSLLTIHEVDDLLTKLSKMTKEEDQQNVLEDIAHRCTSNDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYDAFKASRNLGDVVERVLRNEQQAPGMKRTLSVQASLMTPVQPMLAEACKSIEYAMKKCPNGMYAEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDFIPKAFPGGNSMILDAEVLLIDTNTGKPLPFGTLGVHKKAAFKDANVCLFVFDCIYFNGVSLMDRPLSERRKFMRDNMVEIPNHILFSEMKHVTKASDLADMITRVIREGLEGLVLKDLKSNYEPGKRHWLKVKKDYLNEGAMADTADLAVLGAYYGKGANGGIMSIFLMGCYDPESQKWCTVTKCSSGYDDATLQRLQKELNMVKISKDPSKIPSWLKINKNYFPDFIIHDPKKAPIWEITGAEYSKAEAHTAAGLSIRFPRCTRFRDDKDWKTATTLQQLKELYRLSKEKSDFNITATSSQDEDGSSESSSRENEGNSRQTASGSMVKKSKNAPSKSPGKDKTSESTKLPPPQKKEELPKAEKRKSQTPKTVQSKKVRKQHIEKCIQ

Target ORF information:

RefSeq Version	NM_001016351.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001016351.3

ORF Insert Sequence:

ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT 
GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA 
TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT 
GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG 
GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA 
ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT 
GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT 
CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT 
ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA 
TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA 
AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG 
CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC 
GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG 
TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT 
AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC 
TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA 
GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC 
CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT 
AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT 
GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC 
AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC 
CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT 
CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT 
GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT 
CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA 
GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT 
CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC 
TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT 
AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT 
TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA 
AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT 
TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT 
GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA 
AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA 
CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA 
TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT 
CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG 
TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC 
ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC 
ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT 
GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCTCCT 
TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCTCC TCCTCAGAAG 
AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT 
AAAAAGGTTA GGAAACAACA TATAGAGAAA TGTATCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lig3 ( XM_012971765.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827219.1 Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa27673
Clone ID Related Accession (Same CDS sequence)	XM_012971765.2 , XM_012971764.2 , XM_018097738.1
Accession Version	XM_012971765.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA ligase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971765.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT 
GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA 
TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT 
GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG 
GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA 
ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT 
GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT 
CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT 
ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA 
TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA 
AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG 
CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC 
GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG 
TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT 
AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC 
TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA 
GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC 
CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT 
AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT 
GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC 
AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC 
CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT 
CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT 
GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT 
CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA 
GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT 
CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC 
TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT 
AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT 
TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA 
AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT 
TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT 
GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA 
AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA 
CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA 
TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT 
CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG 
TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC 
ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC 
ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT 
GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCTCCT 
TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCTCC TCCTCAGAAG 
AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT 
AAAAAGGCTA AAATTTCAGA AACTGGTCAC AGTAACGGAC ACAGCATAGC CCAGTCACCT 
GTCACTAATA AAACTCTATT GGACATATTC ACTGGTGTGA AGCTGCATAT TCCATCCACA 
CTTGATGATT TTGCCAAGCT CCGTCGTTAC TTTATCGCCT TTGATGGGGA CCTGGTACCG 
GAATATGATC TTCCCACAGC CACACATGTG ATGGGTCGTG AACCTGAGTG TATTGCAGAT 
GCCAAACATG TAACACCACA CTGGATATGG GAGTGTATAA GACGCAGGCG ATTAGTTGAG 
CCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827219.1
CDS	160..3108
Translation	MRAFLRLLAYQTKRTLGQSLTKVPLRVIFHACWSQSPLHSALHFQFFLRVTHFSNMAEQRYCVEYAKRGTAGCKKCKEKIGKGLVRIGKIVPNPFSESAGDMKEWYHIKCMFEKLERARATTKKIDDLTELEGWQELQDCDKNLISQHVTELATKTAATPRKKTPSKEKQSPAVQGTTSTTAPAASPSLKFSGFSAKPSQSSSPPSSSTGSSLSTAKCDPKHKDCLLREFRKLCAMVAEQPSYNSKTQIIRDFLTKGTSGDGFHGDTYLTVKLLLPGVIKSVYNLNDKQIVKHFSRIFNCNQEEMVRDLEQGDVSETVRIFFEESKCFAPASKSLLTIHEVDDLLTKLSKMTKEEDQQNVLEDIAHRCTSNDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYDAFKASRNLGDVVERVLRNEQQAPGMKRTLSVQASLMTPVQPMLAEACKSIEYAMKKCPNGMYAEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDFIPKAFPGGNSMILDAEVLLIDTNTGKPLPFGTLGVHKKAAFKDANVCLFVFDCIYFNGVSLMDRPLSERRKFMRDNMVEIPNHILFSEMKHVTKASDLADMITRVIREGLEGLVLKDLKSNYEPGKRHWLKVKKDYLNEGAMADTADLAVLGAYYGKGANGGIMSIFLMGCYDPESQKWCTVTKCSSGYDDATLQRLQKELNMVKISKDPSKIPSWLKINKNYFPDFIIHDPKKAPIWEITGAEYSKAEAHTAAGLSIRFPRCTRFRDDKDWKTATTLQQLKELYRLSKEKSDFNITATSSQDEDGSSESSSRENEGNSRQTASGSMVKKSKNAPSKSPGKDKTSESTKLPPPQKKEELPKAEKRKSQTPKTVQSKKAKISETGHSNGHSIAQSPVTNKTLLDIFTGVKLHIPSTLDDFAKLRRYFIAFDGDLVPEYDLPTATHVMGREPECIADAKHVTPHWIWECIRRRRLVEPC

Target ORF information:

RefSeq Version	XM_012971765.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971765.2

ORF Insert Sequence:

ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT 
GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA 
TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT 
GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG 
GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA 
ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT 
GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT 
CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT 
ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA 
TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA 
AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG 
CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC 
GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG 
TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT 
AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC 
TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA 
GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC 
CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT 
AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT 
GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC 
AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC 
CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT 
CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT 
GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT 
CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA 
GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT 
CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC 
TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT 
AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT 
TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA 
AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT 
TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT 
GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA 
AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA 
CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA 
TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT 
CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG 
TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC 
ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC 
ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT 
GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCTCCT 
TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCTCC TCCTCAGAAG 
AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT 
AAAAAGGCTA AAATTTCAGA AACTGGTCAC AGTAACGGAC ACAGCATAGC CCAGTCACCT 
GTCACTAATA AAACTCTATT GGACATATTC ACTGGTGTGA AGCTGCATAT TCCATCCACA 
CTTGATGATT TTGCCAAGCT CCGTCGTTAC TTTATCGCCT TTGATGGGGA CCTGGTACCG 
GAATATGATC TTCCCACAGC CACACATGTG ATGGGTCGTG AACCTGAGTG TATTGCAGAT 
GCCAAACATG TAACACCACA CTGGATATGG GAGTGTATAA GACGCAGGCG ATTAGTTGAG 
CCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lig3 ( XM_012971764.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827218.1 Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa27673
Clone ID Related Accession (Same CDS sequence)	XM_012971765.2 , XM_012971764.2 , XM_018097738.1
Accession Version	XM_012971764.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA ligase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971764.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT 
GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA 
TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT 
GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG 
GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA 
ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT 
GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT 
CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT 
ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA 
TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA 
AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG 
CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC 
GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG 
TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT 
AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC 
TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA 
GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC 
CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT 
AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT 
GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC 
AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC 
CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT 
CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT 
GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT 
CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA 
GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT 
CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC 
TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT 
AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT 
TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA 
AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT 
TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT 
GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA 
AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA 
CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA 
TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT 
CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG 
TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC 
ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC 
ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT 
GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCTCCT 
TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCTCC TCCTCAGAAG 
AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT 
AAAAAGGCTA AAATTTCAGA AACTGGTCAC AGTAACGGAC ACAGCATAGC CCAGTCACCT 
GTCACTAATA AAACTCTATT GGACATATTC ACTGGTGTGA AGCTGCATAT TCCATCCACA 
CTTGATGATT TTGCCAAGCT CCGTCGTTAC TTTATCGCCT TTGATGGGGA CCTGGTACCG 
GAATATGATC TTCCCACAGC CACACATGTG ATGGGTCGTG AACCTGAGTG TATTGCAGAT 
GCCAAACATG TAACACCACA CTGGATATGG GAGTGTATAA GACGCAGGCG ATTAGTTGAG 
CCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827218.1
CDS	153..3101
Translation	MRAFLRLLAYQTKRTLGQSLTKVPLRVIFHACWSQSPLHSALHFQFFLRVTHFSNMAEQRYCVEYAKRGTAGCKKCKEKIGKGLVRIGKIVPNPFSESAGDMKEWYHIKCMFEKLERARATTKKIDDLTELEGWQELQDCDKNLISQHVTELATKTAATPRKKTPSKEKQSPAVQGTTSTTAPAASPSLKFSGFSAKPSQSSSPPSSSTGSSLSTAKCDPKHKDCLLREFRKLCAMVAEQPSYNSKTQIIRDFLTKGTSGDGFHGDTYLTVKLLLPGVIKSVYNLNDKQIVKHFSRIFNCNQEEMVRDLEQGDVSETVRIFFEESKCFAPASKSLLTIHEVDDLLTKLSKMTKEEDQQNVLEDIAHRCTSNDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYDAFKASRNLGDVVERVLRNEQQAPGMKRTLSVQASLMTPVQPMLAEACKSIEYAMKKCPNGMYAEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDFIPKAFPGGNSMILDAEVLLIDTNTGKPLPFGTLGVHKKAAFKDANVCLFVFDCIYFNGVSLMDRPLSERRKFMRDNMVEIPNHILFSEMKHVTKASDLADMITRVIREGLEGLVLKDLKSNYEPGKRHWLKVKKDYLNEGAMADTADLAVLGAYYGKGANGGIMSIFLMGCYDPESQKWCTVTKCSSGYDDATLQRLQKELNMVKISKDPSKIPSWLKINKNYFPDFIIHDPKKAPIWEITGAEYSKAEAHTAAGLSIRFPRCTRFRDDKDWKTATTLQQLKELYRLSKEKSDFNITATSSQDEDGSSESSSRENEGNSRQTASGSMVKKSKNAPSKSPGKDKTSESTKLPPPQKKEELPKAEKRKSQTPKTVQSKKAKISETGHSNGHSIAQSPVTNKTLLDIFTGVKLHIPSTLDDFAKLRRYFIAFDGDLVPEYDLPTATHVMGREPECIADAKHVTPHWIWECIRRRRLVEPC

Target ORF information:

RefSeq Version	XM_012971764.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971764.2

ORF Insert Sequence:

ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT 
GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA 
TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT 
GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG 
GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA 
ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT 
GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT 
CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT 
ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA 
TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA 
AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG 
CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC 
GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG 
TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT 
AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC 
TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA 
GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC 
CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT 
AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT 
GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC 
AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC 
CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT 
CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT 
GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT 
CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA 
GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT 
CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC 
TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT 
AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT 
TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA 
AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT 
TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT 
GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA 
AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA 
CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA 
TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT 
CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG 
TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC 
ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC 
ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT 
GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCTCCT 
TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCTCC TCCTCAGAAG 
AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT 
AAAAAGGCTA AAATTTCAGA AACTGGTCAC AGTAACGGAC ACAGCATAGC CCAGTCACCT 
GTCACTAATA AAACTCTATT GGACATATTC ACTGGTGTGA AGCTGCATAT TCCATCCACA 
CTTGATGATT TTGCCAAGCT CCGTCGTTAC TTTATCGCCT TTGATGGGGA CCTGGTACCG 
GAATATGATC TTCCCACAGC CACACATGTG ATGGGTCGTG AACCTGAGTG TATTGCAGAT 
GCCAAACATG TAACACCACA CTGGATATGG GAGTGTATAA GACGCAGGCG ATTAGTTGAG 
CCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lig3 ( XM_018097738.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017953227.1 Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa27673
Clone ID Related Accession (Same CDS sequence)	XM_012971765.2 , XM_012971764.2 , XM_018097738.1
Accession Version	XM_018097738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA ligase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT 
GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA 
TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT 
GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG 
GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA 
ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT 
GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT 
CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT 
ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA 
TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA 
AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG 
CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC 
GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG 
TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT 
AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC 
TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA 
GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC 
CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT 
AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT 
GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC 
AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC 
CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT 
CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT 
GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT 
CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA 
GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT 
CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC 
TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT 
AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT 
TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA 
AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT 
TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT 
GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA 
AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA 
CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA 
TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT 
CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG 
TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC 
ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC 
ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT 
GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCTCCT 
TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCTCC TCCTCAGAAG 
AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT 
AAAAAGGCTA AAATTTCAGA AACTGGTCAC AGTAACGGAC ACAGCATAGC CCAGTCACCT 
GTCACTAATA AAACTCTATT GGACATATTC ACTGGTGTGA AGCTGCATAT TCCATCCACA 
CTTGATGATT TTGCCAAGCT CCGTCGTTAC TTTATCGCCT TTGATGGGGA CCTGGTACCG 
GAATATGATC TTCCCACAGC CACACATGTG ATGGGTCGTG AACCTGAGTG TATTGCAGAT 
GCCAAACATG TAACACCACA CTGGATATGG GAGTGTATAA GACGCAGGCG ATTAGTTGAG 
CCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017953227.1
CDS	73..3021
Translation	MRAFLRLLAYQTKRTLGQSLTKVPLRVIFHACWSQSPLHSALHFQFFLRVTHFSNMAEQRYCVEYAKRGTAGCKKCKEKIGKGLVRIGKIVPNPFSESAGDMKEWYHIKCMFEKLERARATTKKIDDLTELEGWQELQDCDKNLISQHVTELATKTAATPRKKTPSKEKQSPAVQGTTSTTAPAASPSLKFSGFSAKPSQSSSPPSSSTGSSLSTAKCDPKHKDCLLREFRKLCAMVAEQPSYNSKTQIIRDFLTKGTSGDGFHGDTYLTVKLLLPGVIKSVYNLNDKQIVKHFSRIFNCNQEEMVRDLEQGDVSETVRIFFEESKCFAPASKSLLTIHEVDDLLTKLSKMTKEEDQQNVLEDIAHRCTSNDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYDAFKASRNLGDVVERVLRNEQQAPGMKRTLSVQASLMTPVQPMLAEACKSIEYAMKKCPNGMYAEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDFIPKAFPGGNSMILDAEVLLIDTNTGKPLPFGTLGVHKKAAFKDANVCLFVFDCIYFNGVSLMDRPLSERRKFMRDNMVEIPNHILFSEMKHVTKASDLADMITRVIREGLEGLVLKDLKSNYEPGKRHWLKVKKDYLNEGAMADTADLAVLGAYYGKGANGGIMSIFLMGCYDPESQKWCTVTKCSSGYDDATLQRLQKELNMVKISKDPSKIPSWLKINKNYFPDFIIHDPKKAPIWEITGAEYSKAEAHTAAGLSIRFPRCTRFRDDKDWKTATTLQQLKELYRLSKEKSDFNITATSSQDEDGSSESSSRENEGNSRQTASGSMVKKSKNAPSKSPGKDKTSESTKLPPPQKKEELPKAEKRKSQTPKTVQSKKAKISETGHSNGHSIAQSPVTNKTLLDIFTGVKLHIPSTLDDFAKLRRYFIAFDGDLVPEYDLPTATHVMGREPECIADAKHVTPHWIWECIRRRRLVEPC

Target ORF information:

RefSeq Version	XM_018097738.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097738.1

ORF Insert Sequence:

ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT 
GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA 
TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT 
GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG 
GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA 
ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT 
GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT 
CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT 
ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA 
TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA 
AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG 
CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC 
GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG 
TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT 
AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC 
TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA 
GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC 
CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT 
AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT 
GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC 
AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC 
CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT 
CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT 
GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT 
CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA 
GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT 
CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC 
TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT 
AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT 
TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA 
AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT 
TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT 
GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA 
AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA 
CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA 
TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT 
CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG 
TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC 
ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC 
ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT 
GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCTCCT 
TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCTCC TCCTCAGAAG 
AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT 
AAAAAGGCTA AAATTTCAGA AACTGGTCAC AGTAACGGAC ACAGCATAGC CCAGTCACCT 
GTCACTAATA AAACTCTATT GGACATATTC ACTGGTGTGA AGCTGCATAT TCCATCCACA 
CTTGATGATT TTGCCAAGCT CCGTCGTTAC TTTATCGCCT TTGATGGGGA CCTGGTACCG 
GAATATGATC TTCCCACAGC CACACATGTG ATGGGTCGTG AACCTGAGTG TATTGCAGAT 
GCCAAACATG TAACACCACA CTGGATATGG GAGTGTATAA GACGCAGGCG ATTAGTTGAG 
CCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lig3 ( XM_018097739.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017953228.1 Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa47019
Clone ID Related Accession (Same CDS sequence)	XM_018097739.1
Accession Version	XM_018097739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA ligase 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT 
GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA 
TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT 
GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG 
GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA 
ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT 
GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT 
CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT 
ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA 
TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA 
AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG 
CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC 
GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG 
TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT 
AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC 
TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA 
GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC 
CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT 
AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT 
GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC 
AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC 
CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT 
CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT 
GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT 
CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA 
GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT 
CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC 
TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT 
AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT 
TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA 
AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT 
TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT 
GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA 
AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA 
CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA 
TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT 
CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG 
TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC 
ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC 
ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT 
GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCTCCT 
TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCTCC TCCTCAGAAG 
AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT 
AAAAAGACTC TATTGGACAT ATTCACTGGT GTGAAGCTGC ATATTCCATC CACACTTGAT 
GATTTTGCCA AGCTCCGTCG TTACTTTATC GCCTTTGATG GGGACCTGGT ACCGGAATAT 
GATCTTCCCA CAGCCACACA TGTGATGGGT CGTGAACCTG AGTGTATTGC AGATGCCAAA 
CATGTAACAC CACACTGGAT ATGGGAGTGT ATAAGACGCA GGCGATTAGT TGAGCCATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017953228.1
CDS	153..3035
Translation	MRAFLRLLAYQTKRTLGQSLTKVPLRVIFHACWSQSPLHSALHFQFFLRVTHFSNMAEQRYCVEYAKRGTAGCKKCKEKIGKGLVRIGKIVPNPFSESAGDMKEWYHIKCMFEKLERARATTKKIDDLTELEGWQELQDCDKNLISQHVTELATKTAATPRKKTPSKEKQSPAVQGTTSTTAPAASPSLKFSGFSAKPSQSSSPPSSSTGSSLSTAKCDPKHKDCLLREFRKLCAMVAEQPSYNSKTQIIRDFLTKGTSGDGFHGDTYLTVKLLLPGVIKSVYNLNDKQIVKHFSRIFNCNQEEMVRDLEQGDVSETVRIFFEESKCFAPASKSLLTIHEVDDLLTKLSKMTKEEDQQNVLEDIAHRCTSNDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYDAFKASRNLGDVVERVLRNEQQAPGMKRTLSVQASLMTPVQPMLAEACKSIEYAMKKCPNGMYAEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDFIPKAFPGGNSMILDAEVLLIDTNTGKPLPFGTLGVHKKAAFKDANVCLFVFDCIYFNGVSLMDRPLSERRKFMRDNMVEIPNHILFSEMKHVTKASDLADMITRVIREGLEGLVLKDLKSNYEPGKRHWLKVKKDYLNEGAMADTADLAVLGAYYGKGANGGIMSIFLMGCYDPESQKWCTVTKCSSGYDDATLQRLQKELNMVKISKDPSKIPSWLKINKNYFPDFIIHDPKKAPIWEITGAEYSKAEAHTAAGLSIRFPRCTRFRDDKDWKTATTLQQLKELYRLSKEKSDFNITATSSQDEDGSSESSSRENEGNSRQTASGSMVKKSKNAPSKSPGKDKTSESTKLPPPQKKEELPKAEKRKSQTPKTVQSKKTLLDIFTGVKLHIPSTLDDFAKLRRYFIAFDGDLVPEYDLPTATHVMGREPECIADAKHVTPHWIWECIRRRRLVEPC

Target ORF information:

RefSeq Version	XM_018097739.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097739.1

ORF Insert Sequence:

ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT 
GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA 
TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT 
GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG 
GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA 
ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT 
GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT 
CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT 
ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA 
TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA 
AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG 
CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC 
GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG 
TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT 
AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC 
TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA 
GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC 
CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT 
AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT 
GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC 
AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC 
CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT 
CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT 
GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT 
CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA 
GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT 
CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC 
TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT 
AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT 
TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA 
AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT 
TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT 
GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA 
AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA 
CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA 
TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT 
CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG 
TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC 
ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC 
ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT 
GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCTCCT 
TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCTCC TCCTCAGAAG 
AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT 
AAAAAGACTC TATTGGACAT ATTCACTGGT GTGAAGCTGC ATATTCCATC CACACTTGAT 
GATTTTGCCA AGCTCCGTCG TTACTTTATC GCCTTTGATG GGGACCTGGT ACCGGAATAT 
GATCTTCCCA CAGCCACACA TGTGATGGGT CGTGAACCTG AGTGTATTGC AGATGCCAAA 
CATGTAACAC CACACTGGAT ATGGGAGTGT ATAAGACGCA GGCGATTAGT TGAGCCATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lig3 ( XM_012971765.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827219.2 Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa50081
Clone ID Related Accession (Same CDS sequence)	XM_012971765.3 , XM_012971764.3
Accession Version	XM_012971765.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA ligase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971765.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT 
GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA 
TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT 
GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG 
GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA 
ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT 
GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT 
CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT 
ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA 
TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA 
AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG 
CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC 
GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG 
TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT 
AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC 
TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA 
GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC 
CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT 
AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT 
GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC 
AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC 
CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT 
CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT 
GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT 
CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA 
GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT 
CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC 
TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT 
AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT 
TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA 
AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT 
TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT 
GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA 
AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA 
CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA 
TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT 
CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG 
TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC 
ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC 
ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT 
GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCGCCT 
TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCACC TCCTCAGAAG 
AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT 
AAAAAGGCTA AAATTTCAGA AACTGGTCAC AGTAACGGAC ACAGCATAGC CCAGTCACCT 
GTCGCTAATA AAACTCTATT GGACATATTC ACTGGTGTGA AGCTGCATAT TCCATCCACA 
CTTGATGATT TTGCCAAGCT CCGTCGTTAC TTTATCGCCT TTGATGGGGA CCTGGTACCG 
GAATATGATC TTCCCACAGC CACACATGTG ATGGGTCGTG AACCTGAGTG TATTGCAGAT 
GCCAAACATG TAACACCACA CTGGATATGG GAGTGTATAA GACGCAGGCG ATTAGTTGAG 
CCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827219.2
CDS	91..3039
Translation	MRAFLRLLAYQTKRTLGQSLTKVPLRVIFHACWSQSPLHSALHFQFFLRVTHFSNMAEQRYCVEYAKRGTAGCKKCKEKIGKGLVRIGKIVPNPFSESAGDMKEWYHIKCMFEKLERARATTKKIDDLTELEGWQELQDCDKNLISQHVTELATKTAATPRKKTPSKEKQSPAVQGTTSTTAPAASPSLKFSGFSAKPSQSSSPPSSSTGSSLSTAKCDPKHKDCLLREFRKLCAMVAEQPSYNSKTQIIRDFLTKGTSGDGFHGDTYLTVKLLLPGVIKSVYNLNDKQIVKHFSRIFNCNQEEMVRDLEQGDVSETVRIFFEESKCFAPASKSLLTIHEVDDLLTKLSKMTKEEDQQNVLEDIAHRCTSNDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYDAFKASRNLGDVVERVLRNEQQAPGMKRTLSVQASLMTPVQPMLAEACKSIEYAMKKCPNGMYAEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDFIPKAFPGGNSMILDAEVLLIDTNTGKPLPFGTLGVHKKAAFKDANVCLFVFDCIYFNGVSLMDRPLSERRKFMRDNMVEIPNHILFSEMKHVTKASDLADMITRVIREGLEGLVLKDLKSNYEPGKRHWLKVKKDYLNEGAMADTADLAVLGAYYGKGANGGIMSIFLMGCYDPESQKWCTVTKCSSGYDDATLQRLQKELNMVKISKDPSKIPSWLKINKNYFPDFIIHDPKKAPIWEITGAEYSKAEAHTAAGLSIRFPRCTRFRDDKDWKTATTLQQLKELYRLSKEKSDFNITATSSQDEDGSSESSSRENEGNSRQTASGSMVKKSKNAPSKSPGKDKTSESTKLPPPQKKEELPKAEKRKSQTPKTVQSKKAKISETGHSNGHSIAQSPVANKTLLDIFTGVKLHIPSTLDDFAKLRRYFIAFDGDLVPEYDLPTATHVMGREPECIADAKHVTPHWIWECIRRRRLVEPC

Target ORF information:

RefSeq Version	XM_012971765.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971765.3

ORF Insert Sequence:

ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT 
GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA 
TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT 
GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG 
GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA 
ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT 
GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT 
CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT 
ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA 
TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA 
AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG 
CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC 
GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG 
TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT 
AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC 
TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA 
GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC 
CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT 
AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT 
GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC 
AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC 
CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT 
CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT 
GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT 
CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA 
GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT 
CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC 
TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT 
AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT 
TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA 
AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT 
TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT 
GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA 
AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA 
CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA 
TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT 
CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG 
TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC 
ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC 
ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT 
GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCGCCT 
TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCACC TCCTCAGAAG 
AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT 
AAAAAGGCTA AAATTTCAGA AACTGGTCAC AGTAACGGAC ACAGCATAGC CCAGTCACCT 
GTCGCTAATA AAACTCTATT GGACATATTC ACTGGTGTGA AGCTGCATAT TCCATCCACA 
CTTGATGATT TTGCCAAGCT CCGTCGTTAC TTTATCGCCT TTGATGGGGA CCTGGTACCG 
GAATATGATC TTCCCACAGC CACACATGTG ATGGGTCGTG AACCTGAGTG TATTGCAGAT 
GCCAAACATG TAACACCACA CTGGATATGG GAGTGTATAA GACGCAGGCG ATTAGTTGAG 
CCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lig3 ( XM_012971764.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827218.2 Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X1, mRNA.

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CloneID	OXa50081
Clone ID Related Accession (Same CDS sequence)	XM_012971765.3 , XM_012971764.3
Accession Version	XM_012971764.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA ligase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971764.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT 
GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA 
TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT 
GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG 
GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA 
ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT 
GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT 
CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT 
ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA 
TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA 
AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG 
CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC 
GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG 
TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT 
AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC 
TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA 
GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC 
CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT 
AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT 
GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC 
AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC 
CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT 
CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT 
GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT 
CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA 
GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT 
CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC 
TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT 
AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT 
TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA 
AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT 
TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT 
GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA 
AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA 
CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA 
TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT 
CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG 
TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC 
ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC 
ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT 
GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCGCCT 
TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCACC TCCTCAGAAG 
AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT 
AAAAAGGCTA AAATTTCAGA AACTGGTCAC AGTAACGGAC ACAGCATAGC CCAGTCACCT 
GTCGCTAATA AAACTCTATT GGACATATTC ACTGGTGTGA AGCTGCATAT TCCATCCACA 
CTTGATGATT TTGCCAAGCT CCGTCGTTAC TTTATCGCCT TTGATGGGGA CCTGGTACCG 
GAATATGATC TTCCCACAGC CACACATGTG ATGGGTCGTG AACCTGAGTG TATTGCAGAT 
GCCAAACATG TAACACCACA CTGGATATGG GAGTGTATAA GACGCAGGCG ATTAGTTGAG 
CCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827218.2
CDS	144..3092
Translation	MRAFLRLLAYQTKRTLGQSLTKVPLRVIFHACWSQSPLHSALHFQFFLRVTHFSNMAEQRYCVEYAKRGTAGCKKCKEKIGKGLVRIGKIVPNPFSESAGDMKEWYHIKCMFEKLERARATTKKIDDLTELEGWQELQDCDKNLISQHVTELATKTAATPRKKTPSKEKQSPAVQGTTSTTAPAASPSLKFSGFSAKPSQSSSPPSSSTGSSLSTAKCDPKHKDCLLREFRKLCAMVAEQPSYNSKTQIIRDFLTKGTSGDGFHGDTYLTVKLLLPGVIKSVYNLNDKQIVKHFSRIFNCNQEEMVRDLEQGDVSETVRIFFEESKCFAPASKSLLTIHEVDDLLTKLSKMTKEEDQQNVLEDIAHRCTSNDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYDAFKASRNLGDVVERVLRNEQQAPGMKRTLSVQASLMTPVQPMLAEACKSIEYAMKKCPNGMYAEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDFIPKAFPGGNSMILDAEVLLIDTNTGKPLPFGTLGVHKKAAFKDANVCLFVFDCIYFNGVSLMDRPLSERRKFMRDNMVEIPNHILFSEMKHVTKASDLADMITRVIREGLEGLVLKDLKSNYEPGKRHWLKVKKDYLNEGAMADTADLAVLGAYYGKGANGGIMSIFLMGCYDPESQKWCTVTKCSSGYDDATLQRLQKELNMVKISKDPSKIPSWLKINKNYFPDFIIHDPKKAPIWEITGAEYSKAEAHTAAGLSIRFPRCTRFRDDKDWKTATTLQQLKELYRLSKEKSDFNITATSSQDEDGSSESSSRENEGNSRQTASGSMVKKSKNAPSKSPGKDKTSESTKLPPPQKKEELPKAEKRKSQTPKTVQSKKAKISETGHSNGHSIAQSPVANKTLLDIFTGVKLHIPSTLDDFAKLRRYFIAFDGDLVPEYDLPTATHVMGREPECIADAKHVTPHWIWECIRRRRLVEPC

Target ORF information:

RefSeq Version	XM_012971764.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971764.3

ORF Insert Sequence:

ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT 
GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA 
TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT 
GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG 
GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA 
ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT 
GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT 
CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT 
ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA 
TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA 
AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG 
CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC 
GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG 
TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT 
AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC 
TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA 
GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC 
CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT 
AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT 
GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC 
AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC 
CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT 
CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT 
GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT 
CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA 
GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT 
CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC 
TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT 
AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT 
TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA 
AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT 
TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT 
GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA 
AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA 
CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA 
TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT 
CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG 
TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC 
ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC 
ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT 
GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCGCCT 
TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCACC TCCTCAGAAG 
AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT 
AAAAAGGCTA AAATTTCAGA AACTGGTCAC AGTAACGGAC ACAGCATAGC CCAGTCACCT 
GTCGCTAATA AAACTCTATT GGACATATTC ACTGGTGTGA AGCTGCATAT TCCATCCACA 
CTTGATGATT TTGCCAAGCT CCGTCGTTAC TTTATCGCCT TTGATGGGGA CCTGGTACCG 
GAATATGATC TTCCCACAGC CACACATGTG ATGGGTCGTG AACCTGAGTG TATTGCAGAT 
GCCAAACATG TAACACCACA CTGGATATGG GAGTGTATAA GACGCAGGCG ATTAGTTGAG 
CCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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lig3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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