lig3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following lig3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lig3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa01518 NM_001016351.3
Latest version!
Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa27673 XM_018097738.1
Latest version!
Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa27673 XM_012971765.2
Latest version!
Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa27673 XM_012971764.2
Latest version!
Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa47019 XM_018097739.1
Latest version!
Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OXa50081 XM_012971765.3
Latest version!
Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa50081 XM_012971764.3
Latest version!
Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa01518
    Clone ID Related Accession (Same CDS sequence) NM_001016351.3
    Accession Version NM_001016351.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA ligase 3
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC167946.1. On Dec 29, 2009 this sequence version replaced NM_001016351.2. ##Evidence-Data-START## Transcript exon combination :: BC167946.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
    ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT
    GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA
    TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT
    GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG
    GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA
    ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT
    GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT
    CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT
    ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA
    TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA
    AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG
    CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC
    GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG
    TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT
    AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC
    TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA
    GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC
    CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT
    AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT
    GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC
    AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC
    CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT
    CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT
    GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT
    CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA
    GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT
    CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC
    TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT
    AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT
    TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA
    AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT
    TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT
    GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA
    AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA
    CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA
    TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT
    CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG
    TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC
    ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC
    ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT
    GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCTCCT
    TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCTCC TCCTCAGAAG
    AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT
    AAAAAGGTTA GGAAACAACA TATAGAGAAA TGTATCCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001016351.2
    CDS41..2722
    Translation

    Target ORF information:

    RefSeq Version NM_001016351.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001016351.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
    ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT
    GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA
    TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT
    GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG
    GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA
    ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT
    GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT
    CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT
    ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA
    TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA
    AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG
    CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC
    GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG
    TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT
    AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC
    TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA
    GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC
    CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT
    AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT
    GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC
    AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC
    CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT
    CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT
    GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT
    CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA
    GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT
    CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC
    TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT
    AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT
    TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA
    AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT
    TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT
    GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA
    AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA
    CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA
    TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT
    CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG
    TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC
    ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC
    ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT
    GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCTCCT
    TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCTCC TCCTCAGAAG
    AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT
    AAAAAGGTTA GGAAACAACA TATAGAGAAA TGTATCCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa27673
    Clone ID Related Accession (Same CDS sequence) XM_012971765.2 , XM_012971764.2 , XM_018097738.1
    Accession Version XM_012971765.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA ligase 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971765.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
    ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT
    GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA
    TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT
    GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG
    GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA
    ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT
    GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT
    CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT
    ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA
    TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA
    AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG
    CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC
    GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG
    TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT
    AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC
    TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA
    GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC
    CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT
    AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT
    GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC
    AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC
    CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT
    CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT
    GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT
    CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA
    GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT
    CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC
    TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT
    AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT
    TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA
    AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT
    TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT
    GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA
    AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA
    CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA
    TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT
    CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG
    TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC
    ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC
    ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT
    GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCTCCT
    TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCTCC TCCTCAGAAG
    AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT
    AAAAAGGCTA AAATTTCAGA AACTGGTCAC AGTAACGGAC ACAGCATAGC CCAGTCACCT
    GTCACTAATA AAACTCTATT GGACATATTC ACTGGTGTGA AGCTGCATAT TCCATCCACA
    CTTGATGATT TTGCCAAGCT CCGTCGTTAC TTTATCGCCT TTGATGGGGA CCTGGTACCG
    GAATATGATC TTCCCACAGC CACACATGTG ATGGGTCGTG AACCTGAGTG TATTGCAGAT
    GCCAAACATG TAACACCACA CTGGATATGG GAGTGTATAA GACGCAGGCG ATTAGTTGAG
    CCATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012827219.1
    CDS160..3108
    Translation

    Target ORF information:

    RefSeq Version XM_012971765.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012971765.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
    ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT
    GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA
    TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT
    GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG
    GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA
    ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT
    GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT
    CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT
    ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA
    TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA
    AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG
    CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC
    GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG
    TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT
    AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC
    TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA
    GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC
    CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT
    AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT
    GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC
    AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC
    CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT
    CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT
    GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT
    CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA
    GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT
    CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC
    TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT
    AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT
    TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA
    AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT
    TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT
    GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA
    AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA
    CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA
    TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT
    CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG
    TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC
    ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC
    ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT
    GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCTCCT
    TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCTCC TCCTCAGAAG
    AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT
    AAAAAGGCTA AAATTTCAGA AACTGGTCAC AGTAACGGAC ACAGCATAGC CCAGTCACCT
    GTCACTAATA AAACTCTATT GGACATATTC ACTGGTGTGA AGCTGCATAT TCCATCCACA
    CTTGATGATT TTGCCAAGCT CCGTCGTTAC TTTATCGCCT TTGATGGGGA CCTGGTACCG
    GAATATGATC TTCCCACAGC CACACATGTG ATGGGTCGTG AACCTGAGTG TATTGCAGAT
    GCCAAACATG TAACACCACA CTGGATATGG GAGTGTATAA GACGCAGGCG ATTAGTTGAG
    CCATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa27673
    Clone ID Related Accession (Same CDS sequence) XM_012971765.2 , XM_012971764.2 , XM_018097738.1
    Accession Version XM_012971764.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA ligase 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971764.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
    ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT
    GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA
    TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT
    GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG
    GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA
    ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT
    GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT
    CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT
    ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA
    TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA
    AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG
    CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC
    GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG
    TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT
    AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC
    TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA
    GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC
    CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT
    AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT
    GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC
    AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC
    CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT
    CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT
    GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT
    CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA
    GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT
    CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC
    TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT
    AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT
    TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA
    AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT
    TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT
    GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA
    AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA
    CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA
    TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT
    CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG
    TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC
    ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC
    ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT
    GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCTCCT
    TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCTCC TCCTCAGAAG
    AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT
    AAAAAGGCTA AAATTTCAGA AACTGGTCAC AGTAACGGAC ACAGCATAGC CCAGTCACCT
    GTCACTAATA AAACTCTATT GGACATATTC ACTGGTGTGA AGCTGCATAT TCCATCCACA
    CTTGATGATT TTGCCAAGCT CCGTCGTTAC TTTATCGCCT TTGATGGGGA CCTGGTACCG
    GAATATGATC TTCCCACAGC CACACATGTG ATGGGTCGTG AACCTGAGTG TATTGCAGAT
    GCCAAACATG TAACACCACA CTGGATATGG GAGTGTATAA GACGCAGGCG ATTAGTTGAG
    CCATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012827218.1
    CDS153..3101
    Translation

    Target ORF information:

    RefSeq Version XM_012971764.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012971764.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
    ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT
    GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA
    TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT
    GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG
    GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA
    ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT
    GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT
    CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT
    ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA
    TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA
    AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG
    CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC
    GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG
    TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT
    AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC
    TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA
    GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC
    CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT
    AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT
    GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC
    AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC
    CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT
    CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT
    GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT
    CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA
    GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT
    CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC
    TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT
    AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT
    TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA
    AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT
    TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT
    GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA
    AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA
    CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA
    TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT
    CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG
    TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC
    ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC
    ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT
    GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCTCCT
    TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCTCC TCCTCAGAAG
    AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT
    AAAAAGGCTA AAATTTCAGA AACTGGTCAC AGTAACGGAC ACAGCATAGC CCAGTCACCT
    GTCACTAATA AAACTCTATT GGACATATTC ACTGGTGTGA AGCTGCATAT TCCATCCACA
    CTTGATGATT TTGCCAAGCT CCGTCGTTAC TTTATCGCCT TTGATGGGGA CCTGGTACCG
    GAATATGATC TTCCCACAGC CACACATGTG ATGGGTCGTG AACCTGAGTG TATTGCAGAT
    GCCAAACATG TAACACCACA CTGGATATGG GAGTGTATAA GACGCAGGCG ATTAGTTGAG
    CCATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa27673
    Clone ID Related Accession (Same CDS sequence) XM_012971765.2 , XM_012971764.2 , XM_018097738.1
    Accession Version XM_018097738.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA ligase 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
    ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT
    GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA
    TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT
    GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG
    GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA
    ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT
    GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT
    CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT
    ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA
    TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA
    AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG
    CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC
    GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG
    TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT
    AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC
    TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA
    GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC
    CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT
    AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT
    GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC
    AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC
    CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT
    CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT
    GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT
    CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA
    GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT
    CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC
    TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT
    AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT
    TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA
    AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT
    TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT
    GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA
    AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA
    CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA
    TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT
    CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG
    TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC
    ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC
    ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT
    GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCTCCT
    TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCTCC TCCTCAGAAG
    AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT
    AAAAAGGCTA AAATTTCAGA AACTGGTCAC AGTAACGGAC ACAGCATAGC CCAGTCACCT
    GTCACTAATA AAACTCTATT GGACATATTC ACTGGTGTGA AGCTGCATAT TCCATCCACA
    CTTGATGATT TTGCCAAGCT CCGTCGTTAC TTTATCGCCT TTGATGGGGA CCTGGTACCG
    GAATATGATC TTCCCACAGC CACACATGTG ATGGGTCGTG AACCTGAGTG TATTGCAGAT
    GCCAAACATG TAACACCACA CTGGATATGG GAGTGTATAA GACGCAGGCG ATTAGTTGAG
    CCATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017953227.1
    CDS73..3021
    Translation

    Target ORF information:

    RefSeq Version XM_018097738.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018097738.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
    ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT
    GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA
    TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT
    GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG
    GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA
    ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT
    GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT
    CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT
    ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA
    TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA
    AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG
    CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC
    GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG
    TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT
    AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC
    TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA
    GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC
    CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT
    AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT
    GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC
    AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC
    CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT
    CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT
    GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT
    CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA
    GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT
    CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC
    TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT
    AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT
    TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA
    AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT
    TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT
    GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA
    AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA
    CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA
    TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT
    CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG
    TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC
    ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC
    ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT
    GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCTCCT
    TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCTCC TCCTCAGAAG
    AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT
    AAAAAGGCTA AAATTTCAGA AACTGGTCAC AGTAACGGAC ACAGCATAGC CCAGTCACCT
    GTCACTAATA AAACTCTATT GGACATATTC ACTGGTGTGA AGCTGCATAT TCCATCCACA
    CTTGATGATT TTGCCAAGCT CCGTCGTTAC TTTATCGCCT TTGATGGGGA CCTGGTACCG
    GAATATGATC TTCCCACAGC CACACATGTG ATGGGTCGTG AACCTGAGTG TATTGCAGAT
    GCCAAACATG TAACACCACA CTGGATATGG GAGTGTATAA GACGCAGGCG ATTAGTTGAG
    CCATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47019
    Clone ID Related Accession (Same CDS sequence) XM_018097739.1
    Accession Version XM_018097739.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA ligase 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
    ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT
    GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA
    TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT
    GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG
    GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA
    ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT
    GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT
    CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT
    ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA
    TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA
    AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG
    CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC
    GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG
    TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT
    AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC
    TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA
    GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC
    CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT
    AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT
    GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC
    AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC
    CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT
    CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT
    GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT
    CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA
    GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT
    CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC
    TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT
    AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT
    TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA
    AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT
    TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT
    GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA
    AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA
    CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA
    TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT
    CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG
    TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC
    ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC
    ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT
    GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCTCCT
    TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCTCC TCCTCAGAAG
    AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT
    AAAAAGACTC TATTGGACAT ATTCACTGGT GTGAAGCTGC ATATTCCATC CACACTTGAT
    GATTTTGCCA AGCTCCGTCG TTACTTTATC GCCTTTGATG GGGACCTGGT ACCGGAATAT
    GATCTTCCCA CAGCCACACA TGTGATGGGT CGTGAACCTG AGTGTATTGC AGATGCCAAA
    CATGTAACAC CACACTGGAT ATGGGAGTGT ATAAGACGCA GGCGATTAGT TGAGCCATGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017953228.1
    CDS153..3035
    Translation

    Target ORF information:

    RefSeq Version XM_018097739.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018097739.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
    ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT
    GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA
    TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT
    GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG
    GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA
    ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT
    GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT
    CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT
    ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA
    TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA
    AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG
    CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC
    GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG
    TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT
    AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC
    TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA
    GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC
    CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT
    AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT
    GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC
    AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC
    CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT
    CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT
    GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT
    CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA
    GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT
    CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC
    TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT
    AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT
    TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA
    AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT
    TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT
    GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA
    AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA
    CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA
    TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT
    CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG
    TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC
    ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC
    ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT
    GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCTCCT
    TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCTCC TCCTCAGAAG
    AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT
    AAAAAGACTC TATTGGACAT ATTCACTGGT GTGAAGCTGC ATATTCCATC CACACTTGAT
    GATTTTGCCA AGCTCCGTCG TTACTTTATC GCCTTTGATG GGGACCTGGT ACCGGAATAT
    GATCTTCCCA CAGCCACACA TGTGATGGGT CGTGAACCTG AGTGTATTGC AGATGCCAAA
    CATGTAACAC CACACTGGAT ATGGGAGTGT ATAAGACGCA GGCGATTAGT TGAGCCATGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa50081
    Clone ID Related Accession (Same CDS sequence) XM_012971765.3 , XM_012971764.3
    Accession Version XM_012971765.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA ligase 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971765.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
    ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT
    GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA
    TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT
    GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG
    GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA
    ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT
    GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT
    CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT
    ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA
    TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA
    AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG
    CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC
    GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG
    TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT
    AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC
    TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA
    GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC
    CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT
    AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT
    GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC
    AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC
    CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT
    CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT
    GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT
    CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA
    GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT
    CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC
    TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT
    AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT
    TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA
    AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT
    TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT
    GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA
    AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA
    CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA
    TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT
    CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG
    TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC
    ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC
    ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT
    GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCGCCT
    TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCACC TCCTCAGAAG
    AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT
    AAAAAGGCTA AAATTTCAGA AACTGGTCAC AGTAACGGAC ACAGCATAGC CCAGTCACCT
    GTCGCTAATA AAACTCTATT GGACATATTC ACTGGTGTGA AGCTGCATAT TCCATCCACA
    CTTGATGATT TTGCCAAGCT CCGTCGTTAC TTTATCGCCT TTGATGGGGA CCTGGTACCG
    GAATATGATC TTCCCACAGC CACACATGTG ATGGGTCGTG AACCTGAGTG TATTGCAGAT
    GCCAAACATG TAACACCACA CTGGATATGG GAGTGTATAA GACGCAGGCG ATTAGTTGAG
    CCATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012827219.2
    CDS91..3039
    Translation

    Target ORF information:

    RefSeq Version XM_012971765.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012971765.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
    ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT
    GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA
    TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT
    GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG
    GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA
    ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT
    GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT
    CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT
    ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA
    TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA
    AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG
    CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC
    GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG
    TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT
    AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC
    TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA
    GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC
    CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT
    AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT
    GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC
    AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC
    CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT
    CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT
    GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT
    CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA
    GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT
    CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC
    TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT
    AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT
    TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA
    AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT
    TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT
    GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA
    AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA
    CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA
    TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT
    CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG
    TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC
    ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC
    ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT
    GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCGCCT
    TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCACC TCCTCAGAAG
    AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT
    AAAAAGGCTA AAATTTCAGA AACTGGTCAC AGTAACGGAC ACAGCATAGC CCAGTCACCT
    GTCGCTAATA AAACTCTATT GGACATATTC ACTGGTGTGA AGCTGCATAT TCCATCCACA
    CTTGATGATT TTGCCAAGCT CCGTCGTTAC TTTATCGCCT TTGATGGGGA CCTGGTACCG
    GAATATGATC TTCCCACAGC CACACATGTG ATGGGTCGTG AACCTGAGTG TATTGCAGAT
    GCCAAACATG TAACACCACA CTGGATATGG GAGTGTATAA GACGCAGGCG ATTAGTTGAG
    CCATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa50081
    Clone ID Related Accession (Same CDS sequence) XM_012971765.3 , XM_012971764.3
    Accession Version XM_012971764.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA ligase 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971764.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
    ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT
    GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA
    TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT
    GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG
    GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA
    ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT
    GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT
    CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT
    ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA
    TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA
    AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG
    CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC
    GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG
    TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT
    AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC
    TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA
    GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC
    CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT
    AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT
    GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC
    AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC
    CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT
    CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT
    GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT
    CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA
    GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT
    CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC
    TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT
    AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT
    TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA
    AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT
    TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT
    GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA
    AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA
    CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA
    TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT
    CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG
    TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC
    ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC
    ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT
    GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCGCCT
    TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCACC TCCTCAGAAG
    AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT
    AAAAAGGCTA AAATTTCAGA AACTGGTCAC AGTAACGGAC ACAGCATAGC CCAGTCACCT
    GTCGCTAATA AAACTCTATT GGACATATTC ACTGGTGTGA AGCTGCATAT TCCATCCACA
    CTTGATGATT TTGCCAAGCT CCGTCGTTAC TTTATCGCCT TTGATGGGGA CCTGGTACCG
    GAATATGATC TTCCCACAGC CACACATGTG ATGGGTCGTG AACCTGAGTG TATTGCAGAT
    GCCAAACATG TAACACCACA CTGGATATGG GAGTGTATAA GACGCAGGCG ATTAGTTGAG
    CCATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012827218.2
    CDS144..3092
    Translation

    Target ORF information:

    RefSeq Version XM_012971764.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ligase III, DNA, ATP-dependent (lig3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012971764.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGCAT TTCTTAGACT TCTGGCATAT CAGACCAAAA GGACACTTGG CCAGTCCTTA 
    ACAAAAGTTC CTTTACGAGT TATTTTTCAT GCCTGTTGGA GCCAGTCGCC TCTCCACAGT
    GCTTTGCACT TCCAATTCTT TTTAAGAGTT ACACATTTCT CCAACATGGC GGAACAGCGA
    TACTGTGTGG AATATGCAAA ACGTGGGACT GCTGGTTGCA AGAAATGCAA GGAGAAGATT
    GGAAAGGGTT TGGTACGCAT AGGTAAAATA GTGCCAAACC CTTTTAGCGA GTCTGCTGGG
    GACATGAAGG AGTGGTACCA CATCAAGTGC ATGTTTGAGA AACTGGAGAG AGCGCGGGCA
    ACTACAAAAA AAATTGATGA TCTGACAGAA CTGGAAGGTT GGCAGGAACT TCAAGACTGT
    GATAAAAACC TAATCAGCCA GCATGTCACT GAACTTGCTA CAAAAACAGC AGCTACACCT
    CGAAAAAAGA CTCCGTCCAA GGAAAAACAA TCACCGGCAG TACAAGGAAC AACTTCCACT
    ACAGCACCAG CAGCTAGTCC CTCTCTAAAA TTTTCAGGTT TCTCTGCAAA ACCCAGTCAA
    TCATCAAGCC CACCAAGCTC AAGCACTGGC TCCAGTCTTT CAACTGCAAA GTGTGACCCA
    AAACATAAGG ACTGTCTTCT GCGTGAGTTC CGCAAGCTAT GTGCAATGGT TGCGGAACAG
    CCAAGCTACA ACAGCAAAAC ACAGATCATT CGTGATTTCC TAACCAAGGG AACAAGTGGC
    GATGGTTTTC ATGGTGACAC ATATCTGACA GTAAAACTGC TATTACCAGG AGTTATCAAG
    TCTGTGTACA ACCTCAATGA TAAGCAGATT GTTAAACACT TTAGTCGTAT TTTTAATTGT
    AACCAGGAGG AGATGGTTCG GGACCTAGAA CAGGGAGATG TGTCTGAAAC TGTGCGTATC
    TTCTTTGAGG AAAGTAAATG CTTTGCACCA GCTTCTAAGA GTTTACTGAC AATCCATGAA
    GTTGATGATT TGCTTACTAA ACTATCAAAA ATGACTAAAG AAGAAGATCA ACAGAATGTC
    CTAGAGGACA TTGCTCACAG GTGCACCAGT AATGATCTTA AATGCATTAT CCGCCTGATT
    AAACATGACT TGAAAATGAA CTCTGGAGCC AAACACGTGC TGGATGCTCT AGATCCTAAT
    GCATATGATG CATTTAAAGC TTCGCGCAAT CTAGGGGATG TAGTAGAACG TGTTTTGCGC
    AACGAGCAGC AGGCTCCAGG AATGAAACGA ACTCTTAGTG TCCAGGCTTC ATTAATGACC
    CCAGTTCAGC CTATGCTGGC TGAAGCTTGC AAATCTATAG AGTATGCCAT GAAAAAGTGT
    CCCAATGGTA TGTATGCTGA AATAAAGTAT GATGGTGAAA GAGTCCAAGT ACATAAGAAT
    GGAGATCACT TCAGCTACTT CAGCCGCAGT CTGAAGCCAG TGCTGCCCCA TAAGGTTGCT
    CATTTTAAGG ATTTTATACC TAAAGCATTT CCAGGAGGAA ACAGTATGAT CCTGGATGCA
    GAAGTTCTTC TTATAGATAC CAATACTGGC AAGCCACTTC CATTTGGCAC ATTAGGTGTT
    CATAAGAAAG CTGCTTTCAA AGATGCCAAT GTGTGCTTGT TTGTTTTCGA TTGCATTTAC
    TTCAATGGAG TGAGCTTAAT GGATCGGCCA CTTAGTGAGC GCCGAAAGTT TATGCGGGAT
    AACATGGTTG AAATCCCTAA TCACATTCTG TTCTCTGAGA TGAAACATGT CACAAAAGCT
    TCTGATCTGG CTGACATGAT CACCCGTGTT ATCCGTGAGG GATTGGAGGG TCTGGTATTA
    AAGGACTTAA AGAGTAATTA TGAGCCAGGG AAACGTCATT GGCTTAAAGT AAAGAAGGAT
    TACTTGAATG AAGGTGCAAT GGCAGACACC GCTGACTTGG CAGTTTTGGG AGCTTATTAT
    GGAAAGGGAG CAAATGGGGG TATAATGTCC ATCTTTCTCA TGGGTTGCTA TGATCCTGAA
    AGTCAGAAGT GGTGCACTGT GACAAAATGT TCAAGTGGTT ATGATGATGC TACTCTGCAA
    CGCCTTCAGA AGGAGCTTAA CATGGTCAAA ATCAGCAAGG ATCCATCTAA GATTCCATCA
    TGGCTTAAAA TCAACAAGAA CTATTTCCCA GATTTCATTA TACATGATCC CAAGAAAGCT
    CCCATTTGGG AAATTACAGG TGCAGAGTAC TCAAAGGCAG AGGCTCATAC AGCTGCTGGG
    TTATCCATTC GCTTCCCACG CTGCACACGT TTCCGTGATG ATAAGGACTG GAAAACAGCC
    ACTACACTCC AACAGCTAAA GGAACTTTAT CGTCTCTCAA AAGAAAAATC TGACTTTAAC
    ATCACTGCTA CATCCTCTCA GGATGAGGAT GGTTCATCTG AAAGCAGTAG TCGAGAGAAT
    GAAGGAAACT CCAGGCAAAC TGCATCAGGC AGCATGGTTA AGAAATCGAA AAATGCGCCT
    TCCAAATCTC CTGGAAAGGA CAAAACCAGT GAAAGCACTA AACTACCACC TCCTCAGAAG
    AAAGAAGAGC TGCCGAAAGC AGAGAAGCGC AAATCACAAA CTCCTAAAAC TGTACAGAGT
    AAAAAGGCTA AAATTTCAGA AACTGGTCAC AGTAACGGAC ACAGCATAGC CCAGTCACCT
    GTCGCTAATA AAACTCTATT GGACATATTC ACTGGTGTGA AGCTGCATAT TCCATCCACA
    CTTGATGATT TTGCCAAGCT CCGTCGTTAC TTTATCGCCT TTGATGGGGA CCTGGTACCG
    GAATATGATC TTCCCACAGC CACACATGTG ATGGGTCGTG AACCTGAGTG TATTGCAGAT
    GCCAAACATG TAACACCACA CTGGATATGG GAGTGTATAA GACGCAGGCG ATTAGTTGAG
    CCATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.