atp6v0a2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following atp6v0a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the atp6v0a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa01887 NM_001016223.2
Latest version!
Xenopus tropicalis ATPase H+ transporting V0 subunit a2 (atp6v0a2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa41799 XM_018093085.2
Latest version!
Xenopus tropicalis ATPase H+ transporting V0 subunit a2 (atp6v0a2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa41799 XM_031899739.1
Latest version!
Xenopus tropicalis ATPase H+ transporting V0 subunit a2 (atp6v0a2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa41800 XM_018093086.1
Latest version!
Xenopus tropicalis ATPase, H+ transporting, lysosomal V0 subunit a2 (atp6v0a2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OXa41799 XM_018093085.1 Xenopus tropicalis ATPase, H+ transporting, lysosomal V0 subunit a2 (atp6v0a2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa01887
    Clone ID Related Accession (Same CDS sequence) NM_001016223.2
    Accession Version NM_001016223.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product V-type proton ATPase 116 kDa subunit a
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR926302.2. On Oct 14, 2005 this sequence version replaced NM_001016223.1. ##Evidence-Data-START## Transcript exon combination :: CR926302.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGCTCGT TTTTTCGGAG TGAGGCTATG TGCTTGGCCC AGCTTTTCCT GCAGTCCGGC 
    TCTGCGTATG ACTGTGTCAG CGAGTTGGGG GAAATGGGTC TGGCTGAGTT TAGAGACTTA
    AACCCACAGG TAAGCGCCTT TCAGAGAAAG TTTGTTGGTG AAGTAAAGAA GTGTGAAGAA
    ATGGAGAGAA TACTAGGATA TTTGATGCAA GAAATTCAAA AAGCAAATAT ACCAGTCCCT
    GAGGAATCAT TGACGCCTGA GGCCCCCCTT CCAAAGCATG TGCTTGATAT CCAGGAACAA
    CTGCAAAAAC TGGAAGTGGA GCTGAGGGAA GTGAATAAGA ACAAGGAGAA GCTGCAGAAG
    AACCTTTTAG AAATGATTGA GTATACATAC ATGCTGAGAG TCACAAACTA TTTAGTACAC
    AGATCCAATG AGCATGAAAC TTCTGCACAC AGCACTTATG AGGAATTTCC TGCACTTGAA
    AAGGAATCTT TAATTGACTA TACCTGCATG CAGAGACTTG GGGCAAAACT GGGGTTTGTT
    TCTGGCTTAG TTCACCGTGC AAAGCTGGAA TCCTTTGAGA AAATGCTGTG GAGAGTGTGC
    AAAGGATACA CTGTTTTAAC ATACCAAGAA CTGGATGAGT ATCTTCTGCA TCCAGACACG
    GAAGAACCAA CAAGATGGTT TGTGTTTGTA GTTTCTTACT GGGGAGACCA GATTGGGCAG
    AAGGTGAAAA AAATCTGTGA GTGTTATGAC TGCCATTTAT ATCCATATAC AAACTCTGCT
    GAGGAGAGAA GAAATTTACT GTCTGAGTTA AATACACGTA TTCAGGATTT GCATAAGGTT
    ATTTCTCAAA CAGATGACTA CCTCAAACAG GTTCTATTTA AAGCATCAGA ATCTGTATAC
    AAATGGGTTA TACAGATTAA AAAAATGAAA GCAATTTATC ATGTTCTAAA CTTCTGTCGA
    TTTGATGTCA CCAATAAATG CCTAATTGCT GAGGTCTGGT GTCCAGTAGC TGACCTGCCA
    AACGTTAGAA GAGCACTTGA GGAAGGCTCG AGAAAGAGTG GTGTGTCTGT TCCTTCATTT
    ATAAACCGCA TACCTAGCAA TGACACCCCA CCAACTTTAA TCCGTACTAA CAAGTTCACT
    TCAGGATTTC AAAACATTGT AGATGCTTAT GGAGTTGGAA ACTACAGAGA AATGAATCCA
    GCTCCATACA CTATCATTAC CTTTCCTTTC CTGTTTGCTG TGATGTTTGG AGACTTTGGG
    CATGGGATAT TGATGTCTCT CTTTGCACTC TGTATGGTTG TGTTTGAAAA GAGTCCTAAA
    CTGGCACGTA GTCAAGACGA GATCATGAAG ACCTTTTTTG AAGGCCGCTA CATCATCTTG
    CTGATGGGGC TTTTTTCCAT TTACACTGGG CTAATTTACA ATGACTGTTT TTCCAAGTCA
    ATGAATATCT TTGGCTCAAG CTGGAATGTT TCAGCCATGT TTTCATCAAA CGTTTGGAGG
    ACACAGGATT TATCCAATAA GTTTCTAACA CTGAATCCCA ATGTAACAGG AGTGCTTACA
    GGTGTTTATC CCGTTGGCAT TGATCCAATT TGGAGTTTAT CAACCAATCG CCTCTCTTTC
    CTAAACTCCT TCAAAATGAA GATGTCTGTT ATCCTTGGAG TTATTCATAT GACATTTGGA
    GTTGTTTTGA GTGCCTTTAA CTATATGCAT TTCAAGAAGA GGTTCTGTAT TTTTCTGGTC
    TTCATTCCTG AACTACTTTT TATGATTTGC ATCTTTGGCT ACCTCGTCTT CATGATTTTG
    TACAAGTGGC TGGCGTATTC AGCAGCAGAC TCCAGGAATG CTCCCAGTAT CCTCCTTCAC
    TTTATAAACA TGTTCATGTT TTCTAAAAAT GACCAAATTC CCGCTCTCTT CACTGGGCAG
    GTTGGAATCC AGATATTCCT CATAGTTTTG GTCACTCTTT GTGTACCTGT ACTTCTGTTT
    GGGAAACCTC TCTATCTCTA CTGGCAACAT CATGGCGGAC AGACACTCCG AAATTACAGA
    AAAGGTTACA CCTTAGTACG TCGAGGAAGT GAGGAAGAGA TTTCTCTAGT GAGATCACAT
    GATCTGGAAG AAGGAGAAAT CAACAGCAGC CATGAAGTTC AAAGGGATAG AGATAAGGAA
    AAGTTTGATT TTGCTGATGT GTTCATGAGT CAGGCCATCC ACACCATTGA ATACTGCCTG
    GGATGCATCT CCAATACTGC TTTATACCTT CGTCTCTGGG CACTAAGCTT AGCTCATGCA
    CAGTTGTCAG AAGTACTCTG GGGAATGATC ATGAGGAAGG GTCTCAATGT AGAACCCAGC
    GTTGGGGTCT TTGTGTTGAT ACCACTCTTT GCAGTATTTG CTTTCCTAAC AATTGCTATA
    CTTCTGGTCA TGGAGGGCTT ATCTGCCTTT TTGCATGCAC TCCGATTACA TTGGGTTGAA
    TTTCAGAACA AGTTTTACAC TGGTGCAGGG TACAAATTTA ACCCATTCTC CTTCAAGCAC
    ATCTCTCATA TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001016223.1
    CDS38..2575
    Translation

    Target ORF information:

    RefSeq Version NM_001016223.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase H+ transporting V0 subunit a2 (atp6v0a2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001016223.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGCTCGT TTTTTCGGAG TGAGGCTATG TGCTTGGCCC AGCTTTTCCT GCAGTCCGGC 
    TCTGCGTATG ACTGTGTCAG CGAGTTGGGG GAAATGGGTC TGGCTGAGTT TAGAGACTTA
    AACCCACAGG TAAGCGCCTT TCAGAGAAAG TTTGTTGGTG AAGTAAAGAA GTGTGAAGAA
    ATGGAGAGAA TACTAGGATA TTTGATGCAA GAAATTCAAA AAGCAAATAT ACCAGTCCCT
    GAGGAATCAT TGACGCCTGA GGCCCCCCTT CCAAAGCATG TGCTTGATAT CCAGGAACAA
    CTGCAAAAAC TGGAAGTGGA GCTGAGGGAA GTGAATAAGA ACAAGGAGAA GCTGCAGAAG
    AACCTTTTAG AAATGATTGA GTATACATAC ATGCTGAGAG TCACAAACTA TTTAGTACAC
    AGATCCAATG AGCATGAAAC TTCTGCACAC AGCACTTATG AGGAATTTCC TGCACTTGAA
    AAGGAATCTT TAATTGACTA TACCTGCATG CAGAGACTTG GGGCAAAACT GGGGTTTGTT
    TCTGGCTTAG TTCACCGTGC AAAGCTGGAA TCCTTTGAGA AAATGCTGTG GAGAGTGTGC
    AAAGGATACA CTGTTTTAAC ATACCAAGAA CTGGATGAGT ATCTTCTGCA TCCAGACACG
    GAAGAACCAA CAAGATGGTT TGTGTTTGTA GTTTCTTACT GGGGAGACCA GATTGGGCAG
    AAGGTGAAAA AAATCTGTGA GTGTTATGAC TGCCATTTAT ATCCATATAC AAACTCTGCT
    GAGGAGAGAA GAAATTTACT GTCTGAGTTA AATACACGTA TTCAGGATTT GCATAAGGTT
    ATTTCTCAAA CAGATGACTA CCTCAAACAG GTTCTATTTA AAGCATCAGA ATCTGTATAC
    AAATGGGTTA TACAGATTAA AAAAATGAAA GCAATTTATC ATGTTCTAAA CTTCTGTCGA
    TTTGATGTCA CCAATAAATG CCTAATTGCT GAGGTCTGGT GTCCAGTAGC TGACCTGCCA
    AACGTTAGAA GAGCACTTGA GGAAGGCTCG AGAAAGAGTG GTGTGTCTGT TCCTTCATTT
    ATAAACCGCA TACCTAGCAA TGACACCCCA CCAACTTTAA TCCGTACTAA CAAGTTCACT
    TCAGGATTTC AAAACATTGT AGATGCTTAT GGAGTTGGAA ACTACAGAGA AATGAATCCA
    GCTCCATACA CTATCATTAC CTTTCCTTTC CTGTTTGCTG TGATGTTTGG AGACTTTGGG
    CATGGGATAT TGATGTCTCT CTTTGCACTC TGTATGGTTG TGTTTGAAAA GAGTCCTAAA
    CTGGCACGTA GTCAAGACGA GATCATGAAG ACCTTTTTTG AAGGCCGCTA CATCATCTTG
    CTGATGGGGC TTTTTTCCAT TTACACTGGG CTAATTTACA ATGACTGTTT TTCCAAGTCA
    ATGAATATCT TTGGCTCAAG CTGGAATGTT TCAGCCATGT TTTCATCAAA CGTTTGGAGG
    ACACAGGATT TATCCAATAA GTTTCTAACA CTGAATCCCA ATGTAACAGG AGTGCTTACA
    GGTGTTTATC CCGTTGGCAT TGATCCAATT TGGAGTTTAT CAACCAATCG CCTCTCTTTC
    CTAAACTCCT TCAAAATGAA GATGTCTGTT ATCCTTGGAG TTATTCATAT GACATTTGGA
    GTTGTTTTGA GTGCCTTTAA CTATATGCAT TTCAAGAAGA GGTTCTGTAT TTTTCTGGTC
    TTCATTCCTG AACTACTTTT TATGATTTGC ATCTTTGGCT ACCTCGTCTT CATGATTTTG
    TACAAGTGGC TGGCGTATTC AGCAGCAGAC TCCAGGAATG CTCCCAGTAT CCTCCTTCAC
    TTTATAAACA TGTTCATGTT TTCTAAAAAT GACCAAATTC CCGCTCTCTT CACTGGGCAG
    GTTGGAATCC AGATATTCCT CATAGTTTTG GTCACTCTTT GTGTACCTGT ACTTCTGTTT
    GGGAAACCTC TCTATCTCTA CTGGCAACAT CATGGCGGAC AGACACTCCG AAATTACAGA
    AAAGGTTACA CCTTAGTACG TCGAGGAAGT GAGGAAGAGA TTTCTCTAGT GAGATCACAT
    GATCTGGAAG AAGGAGAAAT CAACAGCAGC CATGAAGTTC AAAGGGATAG AGATAAGGAA
    AAGTTTGATT TTGCTGATGT GTTCATGAGT CAGGCCATCC ACACCATTGA ATACTGCCTG
    GGATGCATCT CCAATACTGC TTTATACCTT CGTCTCTGGG CACTAAGCTT AGCTCATGCA
    CAGTTGTCAG AAGTACTCTG GGGAATGATC ATGAGGAAGG GTCTCAATGT AGAACCCAGC
    GTTGGGGTCT TTGTGTTGAT ACCACTCTTT GCAGTATTTG CTTTCCTAAC AATTGCTATA
    CTTCTGGTCA TGGAGGGCTT ATCTGCCTTT TTGCATGCAC TCCGATTACA TTGGGTTGAA
    TTTCAGAACA AGTTTTACAC TGGTGCAGGG TACAAATTTA ACCCATTCTC CTTCAAGCAC
    ATCTCTCATA TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa41799
    Clone ID Related Accession (Same CDS sequence) XM_018093085.2 , XM_018093085.1 , XM_031899739.1
    Accession Version XM_018093085.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product V-type proton ATPase 116 kDa subunit a isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018093085.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGCTCGT TTTTTCGGAG TGAGGCTATG TGCTTGGCCC AGCTTTTCCT GCAGTCCGGC 
    TCTGCGTATG ACTGTGTCAG CGAGTTGGGG GAAATGGGTC TGGCTGAGTT TAGAGACTTA
    AACCCACAGG TAAGCGCCTT TCAGAGAAAG TTTGTTGGTG AAGTAAAGAA GTGTGAAGAA
    ATGGAGAGAA TACTAGGATA TTTGATGCAA GAAATTCAAA AAGCAAATAT ACCAGTCCCT
    GAGGAATCAT TGACGCCTGA GGCCCCCCTT CCAAAGCATG TGCTTGATAT CCAGGAACAA
    CTGCAAAAAC TGGAAGTGGA GCTGAGGGAA GTGAATAAGA ACAAGGAGAA GCTGCAGAAG
    AACCTTTTAG AAATGATTGA GTATACATAC ATGCTGAGAG TCACAAACTA TTTAGTACAC
    AGATCCAATG AGCATGAAAC TTCTGCACAC AGCACTTATG AGGAATTTCC TGCACTTGAA
    AAGGAATCTT TAATTGACTA TACCTGCATG CAGAGACTTG GGGCAAAACT GGGGTTTGTT
    TCTGGCTTAG TTCACCGTGC AAAGCTGGAA TCCTTTGAGA AAATGCTGTG GAGAGTGTGC
    AAAGGATACA CTGTTTTAAC ATACCAAGAA CTGGATGAGT ATCTTCTGCA TCCAGACACG
    GAAGAACCAA CAAGATGGTT TGTGTTTGTA GTTTCTTACT GGGGAGACCA GATTGGGCAG
    AAGGTGAAAA AAATCTGTGA GTGTTATGAC TGCCATTTAT ATCCATATAC AAACTCTGCT
    GAGGAGAGAA GAAATTTACT GTCTGAGTTA AATACACGTA TTCAGGATTT GCATAAGGTT
    ATTTCTCAAA CAGATGACTA CCTCAAACAG GTTCTATTTA AAGCATCAGA ATCTGTATAC
    AAATGGGTTA TACAGATTAA AAAAATGAAA GCAATTTATC ATGTTCTAAA CTTCTGTCGA
    TTTGATGTCA CCAATAAATG CCTAATTGCT GAGGTCTGGT GTCCAGTAGC TGACCTGCCA
    AACGTTAGAA GAGCACTTGA GGAAGGCTCG AGAAAGAGTG GTGTGTCTGT TCCTTCATTT
    ATAAACCGCA TACCTAGCAA TGACACCCCA CCAACTTTAA TCCGTACTAA CAAGTTCACT
    TCAGGATTTC AAAACATTGT AGATGCTTAT GGAGTTGGAA ACTACAGAGA AATGAATCCA
    GCTCCATACA CTATCATTAC CTTTCCTTTC CTGTTTGCTG TGATGTTTGG AGACTTTGGG
    CATGGGATAT TGATGTCTCT CTTTGCACTC TGTATGGTTG TGTTTGAAAA GAGTCCTAAA
    CTGGCACGTA GTCAAGACGA GATCATGAAG ACCTTTTTTG AAGGCCGCTA CATCATCTTG
    CTGATGGGGC TTTTTTCCAT TTACACTGGG CTAATTTACA ATGACTGTTT TTCCAAGTCA
    ATGAATATCT TTGGCTCAAG CTGGAATGTT TCAGCCATGT TTTCATCAAA CGTTTGGAGG
    ACACAGGATT TATCCAATAA GTTTCTAACA CTGAATCCCA ATGTAACAGG AGTGCTTACA
    GGTGTTTATC CCGTTGGCAT TGATCCAATT TGGAGTTTAT CAACCAATCG CCTCTCTTTC
    CTAAACTCCT TCAAAATGAA GATGTCTGTT ATCCTTGGAG TTATTCATAT GACATTTGGA
    GTTGTTTTGA GTGCCTTTAA CTATATGCAT TTCAAGAAGA GGTTCTGTAT TTTTCTGGTC
    TTCATTCCTG AACTACTTTT TATGATTTGC ATCTTTGGCT ACCTCGTCTT CATGATTTTG
    TACAAGTGGC TGGCGTATTC AGCAGCAGAC TCCAGGAATG CTCCCAGTAT CCTCCTTCAC
    TTTATAAACA TGTTCATGTT TTCTAAAAAT GACCAAATTC CCGCTCTCTT CACTGGGCAG
    GTTGGAATCC AGATATTCCT CATAGTTTTG GTCACTCTTT GTGTACCTGT ACTTCTGTTT
    GGGAAACCTC TCTATCTCTA CTGGCAACAT CATGGCGGAC AGACACTCCG AAATTACAGA
    AAAGGTTACA CCTTAGTACG TCGAGGAAGT GAGGAAGAGA TTTCTCTAGT GAGATCACAT
    GATCTGGAAG AAGGAGAAAT CAACAGCAGC CATGAAGTTC AAAGGGATAG AGATAAGGAA
    AAGTTTGATT TTGCTGATGT GTTCATGAGT CAGGCCATCC ACACCATTGA ATACTGCCTG
    GGATGCATCT CCAATACTGC TTCATACCTT CGTCTCTGGG CACTAAGCTT AGCTCATGCA
    CAGTTGTCAG AAGTACTCTG GGGAATGATC ATGAGGAAGG GTCTCAATGT AGAACCCAGC
    GTTGGGGTCT TTGTGTTGAT ACCACTCTTT GCAGTATTTG CTTTCCTAAC AATTGCTATA
    CTTCTGGTCA TGGAGGGCTT ATCTGCCTTT TTGCATGCAC TCCGATTACA TTGGGTTGAA
    TTTCAGAACA AGTTTTACAC TGGTGCAGGG TACAAATTTA ACCCATTCTC CTTCAAGCAC
    ATCTCTCATA TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948574.1
    CDS160..2697
    Translation

    Target ORF information:

    RefSeq Version XM_018093085.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase H+ transporting V0 subunit a2 (atp6v0a2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018093085.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGCTCGT TTTTTCGGAG TGAGGCTATG TGCTTGGCCC AGCTTTTCCT GCAGTCCGGC 
    TCTGCGTATG ACTGTGTCAG CGAGTTGGGG GAAATGGGTC TGGCTGAGTT TAGAGACTTA
    AACCCACAGG TAAGCGCCTT TCAGAGAAAG TTTGTTGGTG AAGTAAAGAA GTGTGAAGAA
    ATGGAGAGAA TACTAGGATA TTTGATGCAA GAAATTCAAA AAGCAAATAT ACCAGTCCCT
    GAGGAATCAT TGACGCCTGA GGCCCCCCTT CCAAAGCATG TGCTTGATAT CCAGGAACAA
    CTGCAAAAAC TGGAAGTGGA GCTGAGGGAA GTGAATAAGA ACAAGGAGAA GCTGCAGAAG
    AACCTTTTAG AAATGATTGA GTATACATAC ATGCTGAGAG TCACAAACTA TTTAGTACAC
    AGATCCAATG AGCATGAAAC TTCTGCACAC AGCACTTATG AGGAATTTCC TGCACTTGAA
    AAGGAATCTT TAATTGACTA TACCTGCATG CAGAGACTTG GGGCAAAACT GGGGTTTGTT
    TCTGGCTTAG TTCACCGTGC AAAGCTGGAA TCCTTTGAGA AAATGCTGTG GAGAGTGTGC
    AAAGGATACA CTGTTTTAAC ATACCAAGAA CTGGATGAGT ATCTTCTGCA TCCAGACACG
    GAAGAACCAA CAAGATGGTT TGTGTTTGTA GTTTCTTACT GGGGAGACCA GATTGGGCAG
    AAGGTGAAAA AAATCTGTGA GTGTTATGAC TGCCATTTAT ATCCATATAC AAACTCTGCT
    GAGGAGAGAA GAAATTTACT GTCTGAGTTA AATACACGTA TTCAGGATTT GCATAAGGTT
    ATTTCTCAAA CAGATGACTA CCTCAAACAG GTTCTATTTA AAGCATCAGA ATCTGTATAC
    AAATGGGTTA TACAGATTAA AAAAATGAAA GCAATTTATC ATGTTCTAAA CTTCTGTCGA
    TTTGATGTCA CCAATAAATG CCTAATTGCT GAGGTCTGGT GTCCAGTAGC TGACCTGCCA
    AACGTTAGAA GAGCACTTGA GGAAGGCTCG AGAAAGAGTG GTGTGTCTGT TCCTTCATTT
    ATAAACCGCA TACCTAGCAA TGACACCCCA CCAACTTTAA TCCGTACTAA CAAGTTCACT
    TCAGGATTTC AAAACATTGT AGATGCTTAT GGAGTTGGAA ACTACAGAGA AATGAATCCA
    GCTCCATACA CTATCATTAC CTTTCCTTTC CTGTTTGCTG TGATGTTTGG AGACTTTGGG
    CATGGGATAT TGATGTCTCT CTTTGCACTC TGTATGGTTG TGTTTGAAAA GAGTCCTAAA
    CTGGCACGTA GTCAAGACGA GATCATGAAG ACCTTTTTTG AAGGCCGCTA CATCATCTTG
    CTGATGGGGC TTTTTTCCAT TTACACTGGG CTAATTTACA ATGACTGTTT TTCCAAGTCA
    ATGAATATCT TTGGCTCAAG CTGGAATGTT TCAGCCATGT TTTCATCAAA CGTTTGGAGG
    ACACAGGATT TATCCAATAA GTTTCTAACA CTGAATCCCA ATGTAACAGG AGTGCTTACA
    GGTGTTTATC CCGTTGGCAT TGATCCAATT TGGAGTTTAT CAACCAATCG CCTCTCTTTC
    CTAAACTCCT TCAAAATGAA GATGTCTGTT ATCCTTGGAG TTATTCATAT GACATTTGGA
    GTTGTTTTGA GTGCCTTTAA CTATATGCAT TTCAAGAAGA GGTTCTGTAT TTTTCTGGTC
    TTCATTCCTG AACTACTTTT TATGATTTGC ATCTTTGGCT ACCTCGTCTT CATGATTTTG
    TACAAGTGGC TGGCGTATTC AGCAGCAGAC TCCAGGAATG CTCCCAGTAT CCTCCTTCAC
    TTTATAAACA TGTTCATGTT TTCTAAAAAT GACCAAATTC CCGCTCTCTT CACTGGGCAG
    GTTGGAATCC AGATATTCCT CATAGTTTTG GTCACTCTTT GTGTACCTGT ACTTCTGTTT
    GGGAAACCTC TCTATCTCTA CTGGCAACAT CATGGCGGAC AGACACTCCG AAATTACAGA
    AAAGGTTACA CCTTAGTACG TCGAGGAAGT GAGGAAGAGA TTTCTCTAGT GAGATCACAT
    GATCTGGAAG AAGGAGAAAT CAACAGCAGC CATGAAGTTC AAAGGGATAG AGATAAGGAA
    AAGTTTGATT TTGCTGATGT GTTCATGAGT CAGGCCATCC ACACCATTGA ATACTGCCTG
    GGATGCATCT CCAATACTGC TTCATACCTT CGTCTCTGGG CACTAAGCTT AGCTCATGCA
    CAGTTGTCAG AAGTACTCTG GGGAATGATC ATGAGGAAGG GTCTCAATGT AGAACCCAGC
    GTTGGGGTCT TTGTGTTGAT ACCACTCTTT GCAGTATTTG CTTTCCTAAC AATTGCTATA
    CTTCTGGTCA TGGAGGGCTT ATCTGCCTTT TTGCATGCAC TCCGATTACA TTGGGTTGAA
    TTTCAGAACA AGTTTTACAC TGGTGCAGGG TACAAATTTA ACCCATTCTC CTTCAAGCAC
    ATCTCTCATA TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa41799
    Clone ID Related Accession (Same CDS sequence) XM_018093085.2 , XM_018093085.1 , XM_031899739.1
    Accession Version XM_018093085.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product V-type proton ATPase 116 kDa subunit a isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGCTCGT TTTTTCGGAG TGAGGCTATG TGCTTGGCCC AGCTTTTCCT GCAGTCCGGC 
    TCTGCGTATG ACTGTGTCAG CGAGTTGGGG GAAATGGGTC TGGCTGAGTT TAGAGACTTA
    AACCCACAGG TAAGCGCCTT TCAGAGAAAG TTTGTTGGTG AAGTAAAGAA GTGTGAAGAA
    ATGGAGAGAA TACTAGGATA TTTGATGCAA GAAATTCAAA AAGCAAATAT ACCAGTCCCT
    GAGGAATCAT TGACGCCTGA GGCCCCCCTT CCAAAGCATG TGCTTGATAT CCAGGAACAA
    CTGCAAAAAC TGGAAGTGGA GCTGAGGGAA GTGAATAAGA ACAAGGAGAA GCTGCAGAAG
    AACCTTTTAG AAATGATTGA GTATACATAC ATGCTGAGAG TCACAAACTA TTTAGTACAC
    AGATCCAATG AGCATGAAAC TTCTGCACAC AGCACTTATG AGGAATTTCC TGCACTTGAA
    AAGGAATCTT TAATTGACTA TACCTGCATG CAGAGACTTG GGGCAAAACT GGGGTTTGTT
    TCTGGCTTAG TTCACCGTGC AAAGCTGGAA TCCTTTGAGA AAATGCTGTG GAGAGTGTGC
    AAAGGATACA CTGTTTTAAC ATACCAAGAA CTGGATGAGT ATCTTCTGCA TCCAGACACG
    GAAGAACCAA CAAGATGGTT TGTGTTTGTA GTTTCTTACT GGGGAGACCA GATTGGGCAG
    AAGGTGAAAA AAATCTGTGA GTGTTATGAC TGCCATTTAT ATCCATATAC AAACTCTGCT
    GAGGAGAGAA GAAATTTACT GTCTGAGTTA AATACACGTA TTCAGGATTT GCATAAGGTT
    ATTTCTCAAA CAGATGACTA CCTCAAACAG GTTCTATTTA AAGCATCAGA ATCTGTATAC
    AAATGGGTTA TACAGATTAA AAAAATGAAA GCAATTTATC ATGTTCTAAA CTTCTGTCGA
    TTTGATGTCA CCAATAAATG CCTAATTGCT GAGGTCTGGT GTCCAGTAGC TGACCTGCCA
    AACGTTAGAA GAGCACTTGA GGAAGGCTCG AGAAAGAGTG GTGTGTCTGT TCCTTCATTT
    ATAAACCGCA TACCTAGCAA TGACACCCCA CCAACTTTAA TCCGTACTAA CAAGTTCACT
    TCAGGATTTC AAAACATTGT AGATGCTTAT GGAGTTGGAA ACTACAGAGA AATGAATCCA
    GCTCCATACA CTATCATTAC CTTTCCTTTC CTGTTTGCTG TGATGTTTGG AGACTTTGGG
    CATGGGATAT TGATGTCTCT CTTTGCACTC TGTATGGTTG TGTTTGAAAA GAGTCCTAAA
    CTGGCACGTA GTCAAGACGA GATCATGAAG ACCTTTTTTG AAGGCCGCTA CATCATCTTG
    CTGATGGGGC TTTTTTCCAT TTACACTGGG CTAATTTACA ATGACTGTTT TTCCAAGTCA
    ATGAATATCT TTGGCTCAAG CTGGAATGTT TCAGCCATGT TTTCATCAAA CGTTTGGAGG
    ACACAGGATT TATCCAATAA GTTTCTAACA CTGAATCCCA ATGTAACAGG AGTGCTTACA
    GGTGTTTATC CCGTTGGCAT TGATCCAATT TGGAGTTTAT CAACCAATCG CCTCTCTTTC
    CTAAACTCCT TCAAAATGAA GATGTCTGTT ATCCTTGGAG TTATTCATAT GACATTTGGA
    GTTGTTTTGA GTGCCTTTAA CTATATGCAT TTCAAGAAGA GGTTCTGTAT TTTTCTGGTC
    TTCATTCCTG AACTACTTTT TATGATTTGC ATCTTTGGCT ACCTCGTCTT CATGATTTTG
    TACAAGTGGC TGGCGTATTC AGCAGCAGAC TCCAGGAATG CTCCCAGTAT CCTCCTTCAC
    TTTATAAACA TGTTCATGTT TTCTAAAAAT GACCAAATTC CCGCTCTCTT CACTGGGCAG
    GTTGGAATCC AGATATTCCT CATAGTTTTG GTCACTCTTT GTGTACCTGT ACTTCTGTTT
    GGGAAACCTC TCTATCTCTA CTGGCAACAT CATGGCGGAC AGACACTCCG AAATTACAGA
    AAAGGTTACA CCTTAGTACG TCGAGGAAGT GAGGAAGAGA TTTCTCTAGT GAGATCACAT
    GATCTGGAAG AAGGAGAAAT CAACAGCAGC CATGAAGTTC AAAGGGATAG AGATAAGGAA
    AAGTTTGATT TTGCTGATGT GTTCATGAGT CAGGCCATCC ACACCATTGA ATACTGCCTG
    GGATGCATCT CCAATACTGC TTCATACCTT CGTCTCTGGG CACTAAGCTT AGCTCATGCA
    CAGTTGTCAG AAGTACTCTG GGGAATGATC ATGAGGAAGG GTCTCAATGT AGAACCCAGC
    GTTGGGGTCT TTGTGTTGAT ACCACTCTTT GCAGTATTTG CTTTCCTAAC AATTGCTATA
    CTTCTGGTCA TGGAGGGCTT ATCTGCCTTT TTGCATGCAC TCCGATTACA TTGGGTTGAA
    TTTCAGAACA AGTTTTACAC TGGTGCAGGG TACAAATTTA ACCCATTCTC CTTCAAGCAC
    ATCTCTCATA TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948574.1
    CDS216..2753
    Translation

    Target ORF information:

    RefSeq Version XM_018093085.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase, H+ transporting, lysosomal V0 subunit a2 (atp6v0a2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018093085.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGCTCGT TTTTTCGGAG TGAGGCTATG TGCTTGGCCC AGCTTTTCCT GCAGTCCGGC 
    TCTGCGTATG ACTGTGTCAG CGAGTTGGGG GAAATGGGTC TGGCTGAGTT TAGAGACTTA
    AACCCACAGG TAAGCGCCTT TCAGAGAAAG TTTGTTGGTG AAGTAAAGAA GTGTGAAGAA
    ATGGAGAGAA TACTAGGATA TTTGATGCAA GAAATTCAAA AAGCAAATAT ACCAGTCCCT
    GAGGAATCAT TGACGCCTGA GGCCCCCCTT CCAAAGCATG TGCTTGATAT CCAGGAACAA
    CTGCAAAAAC TGGAAGTGGA GCTGAGGGAA GTGAATAAGA ACAAGGAGAA GCTGCAGAAG
    AACCTTTTAG AAATGATTGA GTATACATAC ATGCTGAGAG TCACAAACTA TTTAGTACAC
    AGATCCAATG AGCATGAAAC TTCTGCACAC AGCACTTATG AGGAATTTCC TGCACTTGAA
    AAGGAATCTT TAATTGACTA TACCTGCATG CAGAGACTTG GGGCAAAACT GGGGTTTGTT
    TCTGGCTTAG TTCACCGTGC AAAGCTGGAA TCCTTTGAGA AAATGCTGTG GAGAGTGTGC
    AAAGGATACA CTGTTTTAAC ATACCAAGAA CTGGATGAGT ATCTTCTGCA TCCAGACACG
    GAAGAACCAA CAAGATGGTT TGTGTTTGTA GTTTCTTACT GGGGAGACCA GATTGGGCAG
    AAGGTGAAAA AAATCTGTGA GTGTTATGAC TGCCATTTAT ATCCATATAC AAACTCTGCT
    GAGGAGAGAA GAAATTTACT GTCTGAGTTA AATACACGTA TTCAGGATTT GCATAAGGTT
    ATTTCTCAAA CAGATGACTA CCTCAAACAG GTTCTATTTA AAGCATCAGA ATCTGTATAC
    AAATGGGTTA TACAGATTAA AAAAATGAAA GCAATTTATC ATGTTCTAAA CTTCTGTCGA
    TTTGATGTCA CCAATAAATG CCTAATTGCT GAGGTCTGGT GTCCAGTAGC TGACCTGCCA
    AACGTTAGAA GAGCACTTGA GGAAGGCTCG AGAAAGAGTG GTGTGTCTGT TCCTTCATTT
    ATAAACCGCA TACCTAGCAA TGACACCCCA CCAACTTTAA TCCGTACTAA CAAGTTCACT
    TCAGGATTTC AAAACATTGT AGATGCTTAT GGAGTTGGAA ACTACAGAGA AATGAATCCA
    GCTCCATACA CTATCATTAC CTTTCCTTTC CTGTTTGCTG TGATGTTTGG AGACTTTGGG
    CATGGGATAT TGATGTCTCT CTTTGCACTC TGTATGGTTG TGTTTGAAAA GAGTCCTAAA
    CTGGCACGTA GTCAAGACGA GATCATGAAG ACCTTTTTTG AAGGCCGCTA CATCATCTTG
    CTGATGGGGC TTTTTTCCAT TTACACTGGG CTAATTTACA ATGACTGTTT TTCCAAGTCA
    ATGAATATCT TTGGCTCAAG CTGGAATGTT TCAGCCATGT TTTCATCAAA CGTTTGGAGG
    ACACAGGATT TATCCAATAA GTTTCTAACA CTGAATCCCA ATGTAACAGG AGTGCTTACA
    GGTGTTTATC CCGTTGGCAT TGATCCAATT TGGAGTTTAT CAACCAATCG CCTCTCTTTC
    CTAAACTCCT TCAAAATGAA GATGTCTGTT ATCCTTGGAG TTATTCATAT GACATTTGGA
    GTTGTTTTGA GTGCCTTTAA CTATATGCAT TTCAAGAAGA GGTTCTGTAT TTTTCTGGTC
    TTCATTCCTG AACTACTTTT TATGATTTGC ATCTTTGGCT ACCTCGTCTT CATGATTTTG
    TACAAGTGGC TGGCGTATTC AGCAGCAGAC TCCAGGAATG CTCCCAGTAT CCTCCTTCAC
    TTTATAAACA TGTTCATGTT TTCTAAAAAT GACCAAATTC CCGCTCTCTT CACTGGGCAG
    GTTGGAATCC AGATATTCCT CATAGTTTTG GTCACTCTTT GTGTACCTGT ACTTCTGTTT
    GGGAAACCTC TCTATCTCTA CTGGCAACAT CATGGCGGAC AGACACTCCG AAATTACAGA
    AAAGGTTACA CCTTAGTACG TCGAGGAAGT GAGGAAGAGA TTTCTCTAGT GAGATCACAT
    GATCTGGAAG AAGGAGAAAT CAACAGCAGC CATGAAGTTC AAAGGGATAG AGATAAGGAA
    AAGTTTGATT TTGCTGATGT GTTCATGAGT CAGGCCATCC ACACCATTGA ATACTGCCTG
    GGATGCATCT CCAATACTGC TTCATACCTT CGTCTCTGGG CACTAAGCTT AGCTCATGCA
    CAGTTGTCAG AAGTACTCTG GGGAATGATC ATGAGGAAGG GTCTCAATGT AGAACCCAGC
    GTTGGGGTCT TTGTGTTGAT ACCACTCTTT GCAGTATTTG CTTTCCTAAC AATTGCTATA
    CTTCTGGTCA TGGAGGGCTT ATCTGCCTTT TTGCATGCAC TCCGATTACA TTGGGTTGAA
    TTTCAGAACA AGTTTTACAC TGGTGCAGGG TACAAATTTA ACCCATTCTC CTTCAAGCAC
    ATCTCTCATA TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa41799
    Clone ID Related Accession (Same CDS sequence) XM_018093085.2 , XM_018093085.1 , XM_031899739.1
    Accession Version XM_031899739.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product V-type proton ATPase 116 kDa subunit a isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGCTCGT TTTTTCGGAG TGAGGCTATG TGCTTGGCCC AGCTTTTCCT GCAGTCCGGC 
    TCTGCGTATG ACTGTGTCAG CGAGTTGGGG GAAATGGGTC TGGCTGAGTT TAGAGACTTA
    AACCCACAGG TAAGCGCCTT TCAGAGAAAG TTTGTTGGTG AAGTAAAGAA GTGTGAAGAA
    ATGGAGAGAA TACTAGGATA TTTGATGCAA GAAATTCAAA AAGCAAATAT ACCAGTCCCT
    GAGGAATCAT TGACGCCTGA GGCCCCCCTT CCAAAGCATG TGCTTGATAT CCAGGAACAA
    CTGCAAAAAC TGGAAGTGGA GCTGAGGGAA GTGAATAAGA ACAAGGAGAA GCTGCAGAAG
    AACCTTTTAG AAATGATTGA GTATACATAC ATGCTGAGAG TCACAAACTA TTTAGTACAC
    AGATCCAATG AGCATGAAAC TTCTGCACAC AGCACTTATG AGGAATTTCC TGCACTTGAA
    AAGGAATCTT TAATTGACTA TACCTGCATG CAGAGACTTG GGGCAAAACT GGGGTTTGTT
    TCTGGCTTAG TTCACCGTGC AAAGCTGGAA TCCTTTGAGA AAATGCTGTG GAGAGTGTGC
    AAAGGATACA CTGTTTTAAC ATACCAAGAA CTGGATGAGT ATCTTCTGCA TCCAGACACG
    GAAGAACCAA CAAGATGGTT TGTGTTTGTA GTTTCTTACT GGGGAGACCA GATTGGGCAG
    AAGGTGAAAA AAATCTGTGA GTGTTATGAC TGCCATTTAT ATCCATATAC AAACTCTGCT
    GAGGAGAGAA GAAATTTACT GTCTGAGTTA AATACACGTA TTCAGGATTT GCATAAGGTT
    ATTTCTCAAA CAGATGACTA CCTCAAACAG GTTCTATTTA AAGCATCAGA ATCTGTATAC
    AAATGGGTTA TACAGATTAA AAAAATGAAA GCAATTTATC ATGTTCTAAA CTTCTGTCGA
    TTTGATGTCA CCAATAAATG CCTAATTGCT GAGGTCTGGT GTCCAGTAGC TGACCTGCCA
    AACGTTAGAA GAGCACTTGA GGAAGGCTCG AGAAAGAGTG GTGTGTCTGT TCCTTCATTT
    ATAAACCGCA TACCTAGCAA TGACACCCCA CCAACTTTAA TCCGTACTAA CAAGTTCACT
    TCAGGATTTC AAAACATTGT AGATGCTTAT GGAGTTGGAA ACTACAGAGA AATGAATCCA
    GCTCCATACA CTATCATTAC CTTTCCTTTC CTGTTTGCTG TGATGTTTGG AGACTTTGGG
    CATGGGATAT TGATGTCTCT CTTTGCACTC TGTATGGTTG TGTTTGAAAA GAGTCCTAAA
    CTGGCACGTA GTCAAGACGA GATCATGAAG ACCTTTTTTG AAGGCCGCTA CATCATCTTG
    CTGATGGGGC TTTTTTCCAT TTACACTGGG CTAATTTACA ATGACTGTTT TTCCAAGTCA
    ATGAATATCT TTGGCTCAAG CTGGAATGTT TCAGCCATGT TTTCATCAAA CGTTTGGAGG
    ACACAGGATT TATCCAATAA GTTTCTAACA CTGAATCCCA ATGTAACAGG AGTGCTTACA
    GGTGTTTATC CCGTTGGCAT TGATCCAATT TGGAGTTTAT CAACCAATCG CCTCTCTTTC
    CTAAACTCCT TCAAAATGAA GATGTCTGTT ATCCTTGGAG TTATTCATAT GACATTTGGA
    GTTGTTTTGA GTGCCTTTAA CTATATGCAT TTCAAGAAGA GGTTCTGTAT TTTTCTGGTC
    TTCATTCCTG AACTACTTTT TATGATTTGC ATCTTTGGCT ACCTCGTCTT CATGATTTTG
    TACAAGTGGC TGGCGTATTC AGCAGCAGAC TCCAGGAATG CTCCCAGTAT CCTCCTTCAC
    TTTATAAACA TGTTCATGTT TTCTAAAAAT GACCAAATTC CCGCTCTCTT CACTGGGCAG
    GTTGGAATCC AGATATTCCT CATAGTTTTG GTCACTCTTT GTGTACCTGT ACTTCTGTTT
    GGGAAACCTC TCTATCTCTA CTGGCAACAT CATGGCGGAC AGACACTCCG AAATTACAGA
    AAAGGTTACA CCTTAGTACG TCGAGGAAGT GAGGAAGAGA TTTCTCTAGT GAGATCACAT
    GATCTGGAAG AAGGAGAAAT CAACAGCAGC CATGAAGTTC AAAGGGATAG AGATAAGGAA
    AAGTTTGATT TTGCTGATGT GTTCATGAGT CAGGCCATCC ACACCATTGA ATACTGCCTG
    GGATGCATCT CCAATACTGC TTCATACCTT CGTCTCTGGG CACTAAGCTT AGCTCATGCA
    CAGTTGTCAG AAGTACTCTG GGGAATGATC ATGAGGAAGG GTCTCAATGT AGAACCCAGC
    GTTGGGGTCT TTGTGTTGAT ACCACTCTTT GCAGTATTTG CTTTCCTAAC AATTGCTATA
    CTTCTGGTCA TGGAGGGCTT ATCTGCCTTT TTGCATGCAC TCCGATTACA TTGGGTTGAA
    TTTCAGAACA AGTTTTACAC TGGTGCAGGG TACAAATTTA ACCCATTCTC CTTCAAGCAC
    ATCTCTCATA TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755599.1
    CDS238..2775
    Translation

    Target ORF information:

    RefSeq Version XM_031899739.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase H+ transporting V0 subunit a2 (atp6v0a2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899739.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGCTCGT TTTTTCGGAG TGAGGCTATG TGCTTGGCCC AGCTTTTCCT GCAGTCCGGC 
    TCTGCGTATG ACTGTGTCAG CGAGTTGGGG GAAATGGGTC TGGCTGAGTT TAGAGACTTA
    AACCCACAGG TAAGCGCCTT TCAGAGAAAG TTTGTTGGTG AAGTAAAGAA GTGTGAAGAA
    ATGGAGAGAA TACTAGGATA TTTGATGCAA GAAATTCAAA AAGCAAATAT ACCAGTCCCT
    GAGGAATCAT TGACGCCTGA GGCCCCCCTT CCAAAGCATG TGCTTGATAT CCAGGAACAA
    CTGCAAAAAC TGGAAGTGGA GCTGAGGGAA GTGAATAAGA ACAAGGAGAA GCTGCAGAAG
    AACCTTTTAG AAATGATTGA GTATACATAC ATGCTGAGAG TCACAAACTA TTTAGTACAC
    AGATCCAATG AGCATGAAAC TTCTGCACAC AGCACTTATG AGGAATTTCC TGCACTTGAA
    AAGGAATCTT TAATTGACTA TACCTGCATG CAGAGACTTG GGGCAAAACT GGGGTTTGTT
    TCTGGCTTAG TTCACCGTGC AAAGCTGGAA TCCTTTGAGA AAATGCTGTG GAGAGTGTGC
    AAAGGATACA CTGTTTTAAC ATACCAAGAA CTGGATGAGT ATCTTCTGCA TCCAGACACG
    GAAGAACCAA CAAGATGGTT TGTGTTTGTA GTTTCTTACT GGGGAGACCA GATTGGGCAG
    AAGGTGAAAA AAATCTGTGA GTGTTATGAC TGCCATTTAT ATCCATATAC AAACTCTGCT
    GAGGAGAGAA GAAATTTACT GTCTGAGTTA AATACACGTA TTCAGGATTT GCATAAGGTT
    ATTTCTCAAA CAGATGACTA CCTCAAACAG GTTCTATTTA AAGCATCAGA ATCTGTATAC
    AAATGGGTTA TACAGATTAA AAAAATGAAA GCAATTTATC ATGTTCTAAA CTTCTGTCGA
    TTTGATGTCA CCAATAAATG CCTAATTGCT GAGGTCTGGT GTCCAGTAGC TGACCTGCCA
    AACGTTAGAA GAGCACTTGA GGAAGGCTCG AGAAAGAGTG GTGTGTCTGT TCCTTCATTT
    ATAAACCGCA TACCTAGCAA TGACACCCCA CCAACTTTAA TCCGTACTAA CAAGTTCACT
    TCAGGATTTC AAAACATTGT AGATGCTTAT GGAGTTGGAA ACTACAGAGA AATGAATCCA
    GCTCCATACA CTATCATTAC CTTTCCTTTC CTGTTTGCTG TGATGTTTGG AGACTTTGGG
    CATGGGATAT TGATGTCTCT CTTTGCACTC TGTATGGTTG TGTTTGAAAA GAGTCCTAAA
    CTGGCACGTA GTCAAGACGA GATCATGAAG ACCTTTTTTG AAGGCCGCTA CATCATCTTG
    CTGATGGGGC TTTTTTCCAT TTACACTGGG CTAATTTACA ATGACTGTTT TTCCAAGTCA
    ATGAATATCT TTGGCTCAAG CTGGAATGTT TCAGCCATGT TTTCATCAAA CGTTTGGAGG
    ACACAGGATT TATCCAATAA GTTTCTAACA CTGAATCCCA ATGTAACAGG AGTGCTTACA
    GGTGTTTATC CCGTTGGCAT TGATCCAATT TGGAGTTTAT CAACCAATCG CCTCTCTTTC
    CTAAACTCCT TCAAAATGAA GATGTCTGTT ATCCTTGGAG TTATTCATAT GACATTTGGA
    GTTGTTTTGA GTGCCTTTAA CTATATGCAT TTCAAGAAGA GGTTCTGTAT TTTTCTGGTC
    TTCATTCCTG AACTACTTTT TATGATTTGC ATCTTTGGCT ACCTCGTCTT CATGATTTTG
    TACAAGTGGC TGGCGTATTC AGCAGCAGAC TCCAGGAATG CTCCCAGTAT CCTCCTTCAC
    TTTATAAACA TGTTCATGTT TTCTAAAAAT GACCAAATTC CCGCTCTCTT CACTGGGCAG
    GTTGGAATCC AGATATTCCT CATAGTTTTG GTCACTCTTT GTGTACCTGT ACTTCTGTTT
    GGGAAACCTC TCTATCTCTA CTGGCAACAT CATGGCGGAC AGACACTCCG AAATTACAGA
    AAAGGTTACA CCTTAGTACG TCGAGGAAGT GAGGAAGAGA TTTCTCTAGT GAGATCACAT
    GATCTGGAAG AAGGAGAAAT CAACAGCAGC CATGAAGTTC AAAGGGATAG AGATAAGGAA
    AAGTTTGATT TTGCTGATGT GTTCATGAGT CAGGCCATCC ACACCATTGA ATACTGCCTG
    GGATGCATCT CCAATACTGC TTCATACCTT CGTCTCTGGG CACTAAGCTT AGCTCATGCA
    CAGTTGTCAG AAGTACTCTG GGGAATGATC ATGAGGAAGG GTCTCAATGT AGAACCCAGC
    GTTGGGGTCT TTGTGTTGAT ACCACTCTTT GCAGTATTTG CTTTCCTAAC AATTGCTATA
    CTTCTGGTCA TGGAGGGCTT ATCTGCCTTT TTGCATGCAC TCCGATTACA TTGGGTTGAA
    TTTCAGAACA AGTTTTACAC TGGTGCAGGG TACAAATTTA ACCCATTCTC CTTCAAGCAC
    ATCTCTCATA TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa41800
    Clone ID Related Accession (Same CDS sequence) XM_018093086.1
    Accession Version XM_018093086.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product V-type proton ATPase 116 kDa subunit a isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCTCGT TTTTTCGGAG TGAGGCTATG TGCTTGGCCC AGCTTTTCCT GCAGTCCGGC 
    TCTGCGTATG ACTGTGTCAG CGAGTTGGGG GAAATGGGTC TGGCTGAGTT TAGAGACTTA
    AACCCACAGG TAAGCGCCTT TCAGAGAAAG TTTGTTGGTG AAGTAAAGAA GTGTGAAGAA
    ATGGAGAGAA TACTAGGATA TTTGATGCAA GAAATTCAAA AAGCAAATAT ACCAGTCCCT
    GAGGAATCAT TGACGCCTGA GGCCCCCCTT CCAAAGCATG TGCTTGATAT CCAGGAACAA
    CTGCAAAAAC TGGAAGTGGA GCTGAGGGAA GTGAATAAGA ACAAGGAGAA GCTGCAGAAG
    AACCTTTTAG AAATGATTGA GTATACATAC ATGCTGAGAG TCACAAACTA TTTAGTACAC
    AGATCCAATG AGCATGAAAC TTCTGCACAC AGCACTTATG AGGAATTTCC TGCACTTGAA
    AAGGAATCTT TAATTGACTA TACCTGCATG CAGAGACTTG GGGCAAAACT GGGGTTTGTT
    TCTGGCTTAG TTCACCGTGC AAAGCTGGAA TCCTTTGAGA AAATGCTGTG GAGAGTGTGC
    AAAGGATACA CTGTTTTAAC ATACCAAGAA CTGGATGAGT ATCTTCTGCA TCCAGACACG
    GAAGAACCAA CAAGATGGTT TGTGTTTGTA GTTTCTTACT GGGGAGACCA GATTGGGCAG
    AAGGTGAAAA AAATCTGTGA GTGTTATGAC TGCCATTTAT ATCCATATAC AAACTCTGCT
    GAGGAGAGAA GAAATTTACT GTCTGAGTTA AATACACGTA TTCAGGATTT GCATAAGAGA
    AAGAGTGGTG TGTCTGTTCC TTCATTTATA AACCGCATAC CTAGCAATGA CACCCCACCA
    ACTTTAATCC GTACTAACAA GTTCACTTCA GGATTTCAAA ACATTGTAGA TGCTTATGGA
    GTTGGAAACT ACAGAGAAAT GAATCCAGCT CCATACACTA TCATTACCTT TCCTTTCCTG
    TTTGCTGTGA TGTTTGGAGA CTTTGGGCAT GGGATATTGA TGTCTCTCTT TGCACTCTGT
    ATGGTTGTGT TTGAAAAGAG TCCTAAACTG GCACGTAGTC AAGACGAGAT CATGAAGACC
    TTTTTTGAAG GCCGCTACAT CATCTTGCTG ATGGGGCTTT TTTCCATTTA CACTGGGCTA
    ATTTACAATG ACTGTTTTTC CAAGTCAATG AATATCTTTG GCTCAAGCTG GAATGTTTCA
    GCCATGTTTT CATCAAACGT TTGGAGGACA CAGGATTTAT CCAATAAGTT TCTAACACTG
    AATCCCAATG TAACAGGAGT GCTTACAGGT GTTTATCCCG TTGGCATTGA TCCAATTTGG
    AGTTTATCAA CCAATCGCCT CTCTTTCCTA AACTCCTTCA AAATGAAGAT GTCTGTTATC
    CTTGGAGTTA TTCATATGAC ATTTGGAGTT GTTTTGAGTG CCTTTAACTA TATGCATTTC
    AAGAAGAGGT TCTGTATTTT TCTGGTCTTC ATTCCTGAAC TACTTTTTAT GATTTGCATC
    TTTGGCTACC TCGTCTTCAT GATTTTGTAC AAGTGGCTGG CGTATTCAGC AGCAGACTCC
    AGGAATGCTC CCAGTATCCT CCTTCACTTT ATAAACATGT TCATGTTTTC TAAAAATGAC
    CAAATTCCCG CTCTCTTCAC TGGGCAGGTT GGAATCCAGA TATTCCTCAT AGTTTTGGTC
    ACTCTTTGTG TACCTGTACT TCTGTTTGGG AAACCTCTCT ATCTCTACTG GCAACATCAT
    GGCGGACAGA CACTCCGAAA TTACAGAAAA GGTTACACCT TAGTACGTCG AGGAAGTGAG
    GAAGAGATTT CTCTAGTGAG ATCACATGAT CTGGAAGAAG GAGAAATCAA CAGCAGCCAT
    GAAGTTCAAA GGGATAGAGA TAAGGAAAAG TTTGATTTTG CTGATGTGTT CATGAGTCAG
    GCCATCCACA CCATTGAATA CTGCCTGGGA TGCATCTCCA ATACTGCTTC ATACCTTCGT
    CTCTGGGCAC TAAGCTTAGC TCATGCACAG TTGTCAGAAG TACTCTGGGG AATGATCATG
    AGGAAGGGTC TCAATGTAGA ACCCAGCGTT GGGGTCTTTG TGTTGATACC ACTCTTTGCA
    GTATTTGCTT TCCTAACAAT TGCTATACTT CTGGTCATGG AGGGCTTATC TGCCTTTTTG
    CATGCACTCC GATTACATTG GGTTGAATTT CAGAACAAGT TTTACACTGG TGCAGGGTAC
    AAATTTAACC CATTCTCCTT CAAGCACATC TCTCATATCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948575.1
    CDS170..2494
    Translation

    Target ORF information:

    RefSeq Version XM_018093086.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase, H+ transporting, lysosomal V0 subunit a2 (atp6v0a2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018093086.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCTCGT TTTTTCGGAG TGAGGCTATG TGCTTGGCCC AGCTTTTCCT GCAGTCCGGC 
    TCTGCGTATG ACTGTGTCAG CGAGTTGGGG GAAATGGGTC TGGCTGAGTT TAGAGACTTA
    AACCCACAGG TAAGCGCCTT TCAGAGAAAG TTTGTTGGTG AAGTAAAGAA GTGTGAAGAA
    ATGGAGAGAA TACTAGGATA TTTGATGCAA GAAATTCAAA AAGCAAATAT ACCAGTCCCT
    GAGGAATCAT TGACGCCTGA GGCCCCCCTT CCAAAGCATG TGCTTGATAT CCAGGAACAA
    CTGCAAAAAC TGGAAGTGGA GCTGAGGGAA GTGAATAAGA ACAAGGAGAA GCTGCAGAAG
    AACCTTTTAG AAATGATTGA GTATACATAC ATGCTGAGAG TCACAAACTA TTTAGTACAC
    AGATCCAATG AGCATGAAAC TTCTGCACAC AGCACTTATG AGGAATTTCC TGCACTTGAA
    AAGGAATCTT TAATTGACTA TACCTGCATG CAGAGACTTG GGGCAAAACT GGGGTTTGTT
    TCTGGCTTAG TTCACCGTGC AAAGCTGGAA TCCTTTGAGA AAATGCTGTG GAGAGTGTGC
    AAAGGATACA CTGTTTTAAC ATACCAAGAA CTGGATGAGT ATCTTCTGCA TCCAGACACG
    GAAGAACCAA CAAGATGGTT TGTGTTTGTA GTTTCTTACT GGGGAGACCA GATTGGGCAG
    AAGGTGAAAA AAATCTGTGA GTGTTATGAC TGCCATTTAT ATCCATATAC AAACTCTGCT
    GAGGAGAGAA GAAATTTACT GTCTGAGTTA AATACACGTA TTCAGGATTT GCATAAGAGA
    AAGAGTGGTG TGTCTGTTCC TTCATTTATA AACCGCATAC CTAGCAATGA CACCCCACCA
    ACTTTAATCC GTACTAACAA GTTCACTTCA GGATTTCAAA ACATTGTAGA TGCTTATGGA
    GTTGGAAACT ACAGAGAAAT GAATCCAGCT CCATACACTA TCATTACCTT TCCTTTCCTG
    TTTGCTGTGA TGTTTGGAGA CTTTGGGCAT GGGATATTGA TGTCTCTCTT TGCACTCTGT
    ATGGTTGTGT TTGAAAAGAG TCCTAAACTG GCACGTAGTC AAGACGAGAT CATGAAGACC
    TTTTTTGAAG GCCGCTACAT CATCTTGCTG ATGGGGCTTT TTTCCATTTA CACTGGGCTA
    ATTTACAATG ACTGTTTTTC CAAGTCAATG AATATCTTTG GCTCAAGCTG GAATGTTTCA
    GCCATGTTTT CATCAAACGT TTGGAGGACA CAGGATTTAT CCAATAAGTT TCTAACACTG
    AATCCCAATG TAACAGGAGT GCTTACAGGT GTTTATCCCG TTGGCATTGA TCCAATTTGG
    AGTTTATCAA CCAATCGCCT CTCTTTCCTA AACTCCTTCA AAATGAAGAT GTCTGTTATC
    CTTGGAGTTA TTCATATGAC ATTTGGAGTT GTTTTGAGTG CCTTTAACTA TATGCATTTC
    AAGAAGAGGT TCTGTATTTT TCTGGTCTTC ATTCCTGAAC TACTTTTTAT GATTTGCATC
    TTTGGCTACC TCGTCTTCAT GATTTTGTAC AAGTGGCTGG CGTATTCAGC AGCAGACTCC
    AGGAATGCTC CCAGTATCCT CCTTCACTTT ATAAACATGT TCATGTTTTC TAAAAATGAC
    CAAATTCCCG CTCTCTTCAC TGGGCAGGTT GGAATCCAGA TATTCCTCAT AGTTTTGGTC
    ACTCTTTGTG TACCTGTACT TCTGTTTGGG AAACCTCTCT ATCTCTACTG GCAACATCAT
    GGCGGACAGA CACTCCGAAA TTACAGAAAA GGTTACACCT TAGTACGTCG AGGAAGTGAG
    GAAGAGATTT CTCTAGTGAG ATCACATGAT CTGGAAGAAG GAGAAATCAA CAGCAGCCAT
    GAAGTTCAAA GGGATAGAGA TAAGGAAAAG TTTGATTTTG CTGATGTGTT CATGAGTCAG
    GCCATCCACA CCATTGAATA CTGCCTGGGA TGCATCTCCA ATACTGCTTC ATACCTTCGT
    CTCTGGGCAC TAAGCTTAGC TCATGCACAG TTGTCAGAAG TACTCTGGGG AATGATCATG
    AGGAAGGGTC TCAATGTAGA ACCCAGCGTT GGGGTCTTTG TGTTGATACC ACTCTTTGCA
    GTATTTGCTT TCCTAACAAT TGCTATACTT CTGGTCATGG AGGGCTTATC TGCCTTTTTG
    CATGCACTCC GATTACATTG GGTTGAATTT CAGAACAAGT TTTACACTGG TGCAGGGTAC
    AAATTTAACC CATTCTCCTT CAAGCACATC TCTCATATCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.