ORF » Species Summary » Xenopus tropicalis » bmi1 cDNA ORF clone

bmi1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

  • Gene

  • Clones

  • gRNAs

The following bmi1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bmi1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa02226 NM_001015995.2
Latest version!		 Xenopus tropicalis BMI1 proto-oncogene, polycomb ring finger (bmi1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OXa27516 XM_012964669.2
Latest version!		 Xenopus tropicalis BMI1 proto-oncogene, polycomb ring finger (bmi1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OXa27516 XM_012964670.3
Latest version!		 Xenopus tropicalis BMI1 proto-oncogene, polycomb ring finger (bmi1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
View Old Accession Versions >>
OXa27516 XM_012964670.2 Xenopus tropicalis BMI1 proto-oncogene, polycomb ring finger (bmi1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    bmi1 ( NM_001015995.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001015995.1 Xenopus tropicalis BMI1 proto-oncogene, polycomb ring finger (bmi1), mRNA.
    Price & Availability↑
    CloneID OXa02226
    Clone ID Related Accession (Same CDS sequence) NM_001015995.2
    Accession Version NM_001015995.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 978bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product polycomb complex protein BMI-1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR848302.2. On Oct 14, 2005 this sequence version replaced NM_001015995.1. ##Evidence-Data-START## Transcript exon combination :: CR848302.2, BC157200.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCATCGGA CCACCAGGAT TAAAATTACC GAGTTAAACC CACACCTCAT GTGTGTGCTG 
    TGTGGCGGAT ACTTCATAGA TGCTACAACC ATAATCGAAT GTCTCCATTC CTTTTGTAAA 
    ACCTGCATCG TTCGGTATCT GGAGACCAGC AAATACTGTC CGATCTGTGA TGTTCAAGTA 
    CATAAAACCA GGCCCCTTCT CAACATCAGG GCAGACAAAA CCCTTCAGGA TATTGTGTAT 
    AAGTTGGTGC CAGGGCTTTT CAAAGGTGAA ATGAAGAGGA GAAGAGACTT TTATGCTGCT 
    CATCCTTCTG CAGATGCTTC CAATGGCTCC AACGAGGATA GAGGAGAGGT CGCAGACGAG 
    GACAAGAGGA TCATTACGGA CGATGAGATC ATTAGCTTGT CCATCGAGTT CTTTGACCAA 
    AACAGGGCTG ATCGGAAGGG AAGCAAAGAC AAAGAGAAAT CCAAGGAAGA GGCCAATGAC 
    AAGCGCTACC TGCGCTGCCC AGCCGCCCTC ACAGTCATGC ATCTAAGGAA GTTCCTGAGA 
    AGTAAGATGG ACATCCCCAG CAATTTCCAG ATCGATGTGA TGTACGAGGA GGAACCGCTG 
    AAGGACTATT ACACCTTAAT GGATATTGCT TATATCTACA CCTGGAGAAG GAACGGGCCC 
    CTTCCGTTGA AGTACAGAGT AAGACCGACC TGTAAAAGGA TGAAGATAAA CCCACACACC 
    GACAGGATAA CCAACACTAG CGGGGACATG GAAAGTGACT CTGGCAGCGA CAAAGCGGGC 
    AGCCTGGGTG GCGGCATGCC TTCCACCTCC TCCTGTATGC CAAGCCCCAG CACCCCTGTG 
    CAGTCCCCGC ACCCACAGTT CCCCCACATC TCCAGCACCA TGAATGGAAC CAGCTCCAGC 
    AGCAGCCACC AGAACCCCTT CGCCAACAGA GCAAGGAAAA TATCCTTAAA CGGAGCCTCT 
    GCCATCTCTT CGGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001015995.1
    CDS259..1236
    Translation

    Target ORF information:

    RefSeq Version NM_001015995.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis BMI1 proto-oncogene, polycomb ring finger (bmi1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001015995.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCATCGGA CCACCAGGAT TAAAATTACC GAGTTAAACC CACACCTCAT GTGTGTGCTG 
    TGTGGCGGAT ACTTCATAGA TGCTACAACC ATAATCGAAT GTCTCCATTC CTTTTGTAAA 
    ACCTGCATCG TTCGGTATCT GGAGACCAGC AAATACTGTC CGATCTGTGA TGTTCAAGTA 
    CATAAAACCA GGCCCCTTCT CAACATCAGG GCAGACAAAA CCCTTCAGGA TATTGTGTAT 
    AAGTTGGTGC CAGGGCTTTT CAAAGGTGAA ATGAAGAGGA GAAGAGACTT TTATGCTGCT 
    CATCCTTCTG CAGATGCTTC CAATGGCTCC AACGAGGATA GAGGAGAGGT CGCAGACGAG 
    GACAAGAGGA TCATTACGGA CGATGAGATC ATTAGCTTGT CCATCGAGTT CTTTGACCAA 
    AACAGGGCTG ATCGGAAGGG AAGCAAAGAC AAAGAGAAAT CCAAGGAAGA GGCCAATGAC 
    AAGCGCTACC TGCGCTGCCC AGCCGCCCTC ACAGTCATGC ATCTAAGGAA GTTCCTGAGA 
    AGTAAGATGG ACATCCCCAG CAATTTCCAG ATCGATGTGA TGTACGAGGA GGAACCGCTG 
    AAGGACTATT ACACCTTAAT GGATATTGCT TATATCTACA CCTGGAGAAG GAACGGGCCC 
    CTTCCGTTGA AGTACAGAGT AAGACCGACC TGTAAAAGGA TGAAGATAAA CCCACACACC 
    GACAGGATAA CCAACACTAG CGGGGACATG GAAAGTGACT CTGGCAGCGA CAAAGCGGGC 
    AGCCTGGGTG GCGGCATGCC TTCCACCTCC TCCTGTATGC CAAGCCCCAG CACCCCTGTG 
    CAGTCCCCGC ACCCACAGTT CCCCCACATC TCCAGCACCA TGAATGGAAC CAGCTCCAGC 
    AGCAGCCACC AGAACCCCTT CGCCAACAGA GCAAGGAAAA TATCCTTAAA CGGAGCCTCT 
    GCCATCTCTT CGGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    bmi1 ( XM_012964670.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820124.1 Xenopus tropicalis BMI1 proto-oncogene, polycomb ring finger (bmi1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OXa27516
    Clone ID Related Accession (Same CDS sequence) XM_012964670.3 , XM_012964670.2 , XM_012964669.2
    Accession Version XM_012964670.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 978bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product polycomb complex protein BMI-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964670.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCATCGGA CCACCAGGAT TAAAATTACC GAGTTAAACC CACACCTCAT GTGTGTGCTG 
    TGTGGCGGAT ACTTCATAGA TGCTACAACC ATAATCGAAT GTCTCCATTC CTTTTGTAAA 
    ACCTGCATCG TTCGGTATCT GGAGACCAGC AAATACTGTC CGATCTGTGA TGTTCAAGTA 
    CATAAAACCA GGCCCCTTCT CAACATCAGG GCAGACAAAA CCCTTCAGGA TATTGTGTAT 
    AAGTTGGTGC CAGGGCTTTT CAAAGGTGAA ATGAAGAGGA GAAGAGACTT TTATGCTGCT 
    CATCCTTCTG CAGATGCTTC CAATGGCTCC AACGAGGATA GAGGAGAGGT CGCAGACGAG 
    GACAAGAGGA TCATTACGGA CGATGAGATC ATTAGCTTGT CCATCGAGTT CTTTGACCAA 
    AACAGGGCTG ATCGGAAGGG AAGCAAAGAC AAAGAGAAAT CCAAGGAAGA GGCCAATGAC 
    AAGCGCTACC TGCGCTGCCC AGCCGCCCTC ACAGTCATGC ATCTAAGGAA GTTCCTGAGA 
    AGTAAGATGG ACATCCCCAG CAATTTCCAG ATCGATGTGA TGTACGAGGA GGAACCGCTG 
    AAGGACTATT ACACCTTAAT GGATATTGCT TATATCTACA CCTGGAGAAG GAACGGGCCC 
    CTTCCGTTGA AGTACAGAGT AAGACCGACC TGTAAAAGAA TGAAGATAAA CCCACACACC 
    GACAGGATAA CCAACACTAG CGGGGACATG GAAAGTGACT CTGGCAGCGA CAAAGCGGGC 
    AGCCTGGGTG GCGGCATGCC TTCCACCTCC TCCTGTATGC CAAGCCCCAG CACCCCTGTG 
    CAGTCCCCGC ACCCACAGTT CCCCCACATC TCCAGCACCA TGAATGGAAC CAGCTCCAGC 
    AGCAGCCACC AGAACCCCTT CGCCAACAGA GCAAGGAAAA TATCCTTAAA CGGAGCCTCT 
    GCCATCTCTT CGGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820124.1
    CDS1506..2483
    Translation

    Target ORF information:

    RefSeq Version XM_012964670.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis BMI1 proto-oncogene, polycomb ring finger (bmi1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012964670.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCATCGGA CCACCAGGAT TAAAATTACC GAGTTAAACC CACACCTCAT GTGTGTGCTG 
    TGTGGCGGAT ACTTCATAGA TGCTACAACC ATAATCGAAT GTCTCCATTC CTTTTGTAAA 
    ACCTGCATCG TTCGGTATCT GGAGACCAGC AAATACTGTC CGATCTGTGA TGTTCAAGTA 
    CATAAAACCA GGCCCCTTCT CAACATCAGG GCAGACAAAA CCCTTCAGGA TATTGTGTAT 
    AAGTTGGTGC CAGGGCTTTT CAAAGGTGAA ATGAAGAGGA GAAGAGACTT TTATGCTGCT 
    CATCCTTCTG CAGATGCTTC CAATGGCTCC AACGAGGATA GAGGAGAGGT CGCAGACGAG 
    GACAAGAGGA TCATTACGGA CGATGAGATC ATTAGCTTGT CCATCGAGTT CTTTGACCAA 
    AACAGGGCTG ATCGGAAGGG AAGCAAAGAC AAAGAGAAAT CCAAGGAAGA GGCCAATGAC 
    AAGCGCTACC TGCGCTGCCC AGCCGCCCTC ACAGTCATGC ATCTAAGGAA GTTCCTGAGA 
    AGTAAGATGG ACATCCCCAG CAATTTCCAG ATCGATGTGA TGTACGAGGA GGAACCGCTG 
    AAGGACTATT ACACCTTAAT GGATATTGCT TATATCTACA CCTGGAGAAG GAACGGGCCC 
    CTTCCGTTGA AGTACAGAGT AAGACCGACC TGTAAAAGAA TGAAGATAAA CCCACACACC 
    GACAGGATAA CCAACACTAG CGGGGACATG GAAAGTGACT CTGGCAGCGA CAAAGCGGGC 
    AGCCTGGGTG GCGGCATGCC TTCCACCTCC TCCTGTATGC CAAGCCCCAG CACCCCTGTG 
    CAGTCCCCGC ACCCACAGTT CCCCCACATC TCCAGCACCA TGAATGGAAC CAGCTCCAGC 
    AGCAGCCACC AGAACCCCTT CGCCAACAGA GCAAGGAAAA TATCCTTAAA CGGAGCCTCT 
    GCCATCTCTT CGGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    bmi1 ( XM_012964669.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820123.1 Xenopus tropicalis BMI1 proto-oncogene, polycomb ring finger (bmi1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OXa27516
    Clone ID Related Accession (Same CDS sequence) XM_012964670.3 , XM_012964670.2 , XM_012964669.2
    Accession Version XM_012964669.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 978bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product polycomb complex protein BMI-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964669.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCATCGGA CCACCAGGAT TAAAATTACC GAGTTAAACC CACACCTCAT GTGTGTGCTG 
    TGTGGCGGAT ACTTCATAGA TGCTACAACC ATAATCGAAT GTCTCCATTC CTTTTGTAAA 
    ACCTGCATCG TTCGGTATCT GGAGACCAGC AAATACTGTC CGATCTGTGA TGTTCAAGTA 
    CATAAAACCA GGCCCCTTCT CAACATCAGG GCAGACAAAA CCCTTCAGGA TATTGTGTAT 
    AAGTTGGTGC CAGGGCTTTT CAAAGGTGAA ATGAAGAGGA GAAGAGACTT TTATGCTGCT 
    CATCCTTCTG CAGATGCTTC CAATGGCTCC AACGAGGATA GAGGAGAGGT CGCAGACGAG 
    GACAAGAGGA TCATTACGGA CGATGAGATC ATTAGCTTGT CCATCGAGTT CTTTGACCAA 
    AACAGGGCTG ATCGGAAGGG AAGCAAAGAC AAAGAGAAAT CCAAGGAAGA GGCCAATGAC 
    AAGCGCTACC TGCGCTGCCC AGCCGCCCTC ACAGTCATGC ATCTAAGGAA GTTCCTGAGA 
    AGTAAGATGG ACATCCCCAG CAATTTCCAG ATCGATGTGA TGTACGAGGA GGAACCGCTG 
    AAGGACTATT ACACCTTAAT GGATATTGCT TATATCTACA CCTGGAGAAG GAACGGGCCC 
    CTTCCGTTGA AGTACAGAGT AAGACCGACC TGTAAAAGAA TGAAGATAAA CCCACACACC 
    GACAGGATAA CCAACACTAG CGGGGACATG GAAAGTGACT CTGGCAGCGA CAAAGCGGGC 
    AGCCTGGGTG GCGGCATGCC TTCCACCTCC TCCTGTATGC CAAGCCCCAG CACCCCTGTG 
    CAGTCCCCGC ACCCACAGTT CCCCCACATC TCCAGCACCA TGAATGGAAC CAGCTCCAGC 
    AGCAGCCACC AGAACCCCTT CGCCAACAGA GCAAGGAAAA TATCCTTAAA CGGAGCCTCT 
    GCCATCTCTT CGGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820123.1
    CDS499..1476
    Translation

    Target ORF information:

    RefSeq Version XM_012964669.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis BMI1 proto-oncogene, polycomb ring finger (bmi1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012964669.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCATCGGA CCACCAGGAT TAAAATTACC GAGTTAAACC CACACCTCAT GTGTGTGCTG 
    TGTGGCGGAT ACTTCATAGA TGCTACAACC ATAATCGAAT GTCTCCATTC CTTTTGTAAA 
    ACCTGCATCG TTCGGTATCT GGAGACCAGC AAATACTGTC CGATCTGTGA TGTTCAAGTA 
    CATAAAACCA GGCCCCTTCT CAACATCAGG GCAGACAAAA CCCTTCAGGA TATTGTGTAT 
    AAGTTGGTGC CAGGGCTTTT CAAAGGTGAA ATGAAGAGGA GAAGAGACTT TTATGCTGCT 
    CATCCTTCTG CAGATGCTTC CAATGGCTCC AACGAGGATA GAGGAGAGGT CGCAGACGAG 
    GACAAGAGGA TCATTACGGA CGATGAGATC ATTAGCTTGT CCATCGAGTT CTTTGACCAA 
    AACAGGGCTG ATCGGAAGGG AAGCAAAGAC AAAGAGAAAT CCAAGGAAGA GGCCAATGAC 
    AAGCGCTACC TGCGCTGCCC AGCCGCCCTC ACAGTCATGC ATCTAAGGAA GTTCCTGAGA 
    AGTAAGATGG ACATCCCCAG CAATTTCCAG ATCGATGTGA TGTACGAGGA GGAACCGCTG 
    AAGGACTATT ACACCTTAAT GGATATTGCT TATATCTACA CCTGGAGAAG GAACGGGCCC 
    CTTCCGTTGA AGTACAGAGT AAGACCGACC TGTAAAAGAA TGAAGATAAA CCCACACACC 
    GACAGGATAA CCAACACTAG CGGGGACATG GAAAGTGACT CTGGCAGCGA CAAAGCGGGC 
    AGCCTGGGTG GCGGCATGCC TTCCACCTCC TCCTGTATGC CAAGCCCCAG CACCCCTGTG 
    CAGTCCCCGC ACCCACAGTT CCCCCACATC TCCAGCACCA TGAATGGAAC CAGCTCCAGC 
    AGCAGCCACC AGAACCCCTT CGCCAACAGA GCAAGGAAAA TATCCTTAAA CGGAGCCTCT 
    GCCATCTCTT CGGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    bmi1 ( XM_012964670.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820124.1 Xenopus tropicalis BMI1 proto-oncogene, polycomb ring finger (bmi1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OXa27516
    Clone ID Related Accession (Same CDS sequence) XM_012964670.3 , XM_012964670.2 , XM_012964669.2
    Accession Version XM_012964670.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 978bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product polycomb complex protein BMI-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964670.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCATCGGA CCACCAGGAT TAAAATTACC GAGTTAAACC CACACCTCAT GTGTGTGCTG 
    TGTGGCGGAT ACTTCATAGA TGCTACAACC ATAATCGAAT GTCTCCATTC CTTTTGTAAA 
    ACCTGCATCG TTCGGTATCT GGAGACCAGC AAATACTGTC CGATCTGTGA TGTTCAAGTA 
    CATAAAACCA GGCCCCTTCT CAACATCAGG GCAGACAAAA CCCTTCAGGA TATTGTGTAT 
    AAGTTGGTGC CAGGGCTTTT CAAAGGTGAA ATGAAGAGGA GAAGAGACTT TTATGCTGCT 
    CATCCTTCTG CAGATGCTTC CAATGGCTCC AACGAGGATA GAGGAGAGGT CGCAGACGAG 
    GACAAGAGGA TCATTACGGA CGATGAGATC ATTAGCTTGT CCATCGAGTT CTTTGACCAA 
    AACAGGGCTG ATCGGAAGGG AAGCAAAGAC AAAGAGAAAT CCAAGGAAGA GGCCAATGAC 
    AAGCGCTACC TGCGCTGCCC AGCCGCCCTC ACAGTCATGC ATCTAAGGAA GTTCCTGAGA 
    AGTAAGATGG ACATCCCCAG CAATTTCCAG ATCGATGTGA TGTACGAGGA GGAACCGCTG 
    AAGGACTATT ACACCTTAAT GGATATTGCT TATATCTACA CCTGGAGAAG GAACGGGCCC 
    CTTCCGTTGA AGTACAGAGT AAGACCGACC TGTAAAAGAA TGAAGATAAA CCCACACACC 
    GACAGGATAA CCAACACTAG CGGGGACATG GAAAGTGACT CTGGCAGCGA CAAAGCGGGC 
    AGCCTGGGTG GCGGCATGCC TTCCACCTCC TCCTGTATGC CAAGCCCCAG CACCCCTGTG 
    CAGTCCCCGC ACCCACAGTT CCCCCACATC TCCAGCACCA TGAATGGAAC CAGCTCCAGC 
    AGCAGCCACC AGAACCCCTT CGCCAACAGA GCAAGGAAAA TATCCTTAAA CGGAGCCTCT 
    GCCATCTCTT CGGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820124.1
    CDS511..1488
    Translation

    Target ORF information:

    RefSeq Version XM_012964670.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis BMI1 proto-oncogene, polycomb ring finger (bmi1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012964670.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCATCGGA CCACCAGGAT TAAAATTACC GAGTTAAACC CACACCTCAT GTGTGTGCTG 
    TGTGGCGGAT ACTTCATAGA TGCTACAACC ATAATCGAAT GTCTCCATTC CTTTTGTAAA 
    ACCTGCATCG TTCGGTATCT GGAGACCAGC AAATACTGTC CGATCTGTGA TGTTCAAGTA 
    CATAAAACCA GGCCCCTTCT CAACATCAGG GCAGACAAAA CCCTTCAGGA TATTGTGTAT 
    AAGTTGGTGC CAGGGCTTTT CAAAGGTGAA ATGAAGAGGA GAAGAGACTT TTATGCTGCT 
    CATCCTTCTG CAGATGCTTC CAATGGCTCC AACGAGGATA GAGGAGAGGT CGCAGACGAG 
    GACAAGAGGA TCATTACGGA CGATGAGATC ATTAGCTTGT CCATCGAGTT CTTTGACCAA 
    AACAGGGCTG ATCGGAAGGG AAGCAAAGAC AAAGAGAAAT CCAAGGAAGA GGCCAATGAC 
    AAGCGCTACC TGCGCTGCCC AGCCGCCCTC ACAGTCATGC ATCTAAGGAA GTTCCTGAGA 
    AGTAAGATGG ACATCCCCAG CAATTTCCAG ATCGATGTGA TGTACGAGGA GGAACCGCTG 
    AAGGACTATT ACACCTTAAT GGATATTGCT TATATCTACA CCTGGAGAAG GAACGGGCCC 
    CTTCCGTTGA AGTACAGAGT AAGACCGACC TGTAAAAGAA TGAAGATAAA CCCACACACC 
    GACAGGATAA CCAACACTAG CGGGGACATG GAAAGTGACT CTGGCAGCGA CAAAGCGGGC 
    AGCCTGGGTG GCGGCATGCC TTCCACCTCC TCCTGTATGC CAAGCCCCAG CACCCCTGTG 
    CAGTCCCCGC ACCCACAGTT CCCCCACATC TCCAGCACCA TGAATGGAAC CAGCTCCAGC 
    AGCAGCCACC AGAACCCCTT CGCCAACAGA GCAAGGAAAA TATCCTTAAA CGGAGCCTCT 
    GCCATCTCTT CGGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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