sh3rf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following sh3rf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sh3rf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa25769 NM_001015973.2
Latest version!
Xenopus tropicalis SH3 domain containing ring finger 1 (sh3rf1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa27509 XM_012961866.3
Latest version!
Xenopus tropicalis SH3 domain containing ring finger 1 (sh3rf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa27510 XM_012961878.3
Latest version!
Xenopus tropicalis SH3 domain containing ring finger 1 (sh3rf1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa27510 XM_012961871.3
Latest version!
Xenopus tropicalis SH3 domain containing ring finger 1 (sh3rf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa41795 XM_018093029.1
Latest version!
Xenopus tropicalis SH3 domain containing ring finger 1 (sh3rf1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OXa41796 XM_018093030.1
Latest version!
Xenopus tropicalis SH3 domain containing ring finger 1 (sh3rf1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OXa27510 XM_012961878.2 Xenopus tropicalis SH3 domain containing ring finger 1 (sh3rf1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa27510 XM_012961871.2 Xenopus tropicalis SH3 domain containing ring finger 1 (sh3rf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa27509 XM_012961866.2 Xenopus tropicalis SH3 domain containing ring finger 1 (sh3rf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa25769
    Clone ID Related Accession (Same CDS sequence) NM_001015973.2
    Accession Version NM_001015973.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2586bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase SH3RF1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR848380.2. On Oct 14, 2005 this sequence version replaced NM_001015973.1. ##Evidence-Data-START## Transcript exon combination :: CR848380.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGATGAGT CAGCATTACT GGACCTCTTA GAATGTCCAG TGTGTCTTGA GAGACTTGAT 
    GCCTCTGCTA AAGTCTTGCC ATGCCAACAC ACATTCTGCA AGCGCTGCCT TCTGGGCATT
    GTGAGCTCTC GCAATGAGCT GCGCTGCCCA GAGTGCAGGA CTCTGGTGGA GTGTGGGGTG
    GATGAACTTC CGAGCAACAT CCTACTAGTG AGACTGTTGG ATGGAATAAA GCAGAGACCA
    AGAAAAGCAG GGGTTGGTGG CAGTGCTGGG AATAGCACAA ATGTGCTAAG AGCACAAGGC
    AGTCTAACTA CTAATTGTGG CTTAAATGAT GCACAGAATA TTCATGGTGG GCAACAGAGG
    ATACAGGCAC GAAGTCCTCC AGTAAGGGGT GTTCCTCAGT TACCATGTGC CAAAGCTTTG
    TACAACTACG AGGGGAAAGA GCCTGGAGAT CTGAAATTTA ACAAGGGAGA CATCATCGTG
    CTTCGCCGTC AGGTTGATGA AAATTGGTAC CACGGGGAGA TTAATGGCAT CCATGGCTTC
    TTCCCAACAA ACTTTGTGCA GATCATAAAG CCTTTACCTC AGCCTCCTCC TCAATGCAAA
    GCACTTTATG ACTTTGAAGT GAAAGACAAG GAAGCAGACA AGGATTGCCT ACCCTTTTTA
    AAGGATGACA TTCTCACCGT GATCCGACGA GTAGATGAAA ACTGGGCAGA AGGAATGCTG
    GGAGACAAGA TAGGAATATT CCCTATTTCC TATGTAGAGT TCAATTCAGC AGCCAAGCAG
    CTGATAGAAT TGGACAAGCC ATCAGGAGTA GACACTGGGG AAGGCTCTTC TGGCACAACG
    CATAGTAGCA ATTCTCAAAA GCAAGCAGAT GCAAAGAAGA ACACCAAAAA GCGTCACTCC
    TTTACCTCCC TCACCATGTC TAACAAGTCA TCGCAGTCCG TGCAGAATCG TCATTCAATG
    GAGATTAGCC CCCCTGTACT CATCAGTTCT AGCAATCCCA CCGCTGCTGC CAGGATCAGT
    GAACTCACAG GCCTCTCTTG TAGTGCCCCT TCTCAGGTTC ATATCAGTAC AACAGGGCTC
    ATTGTGACCC CACCCCCTAG CAGCCCTGTT GTAAGTGGCC CTGCATTTAC ATTTCCACCG
    GAAGTCACCT ACCAAGCTGC TCTGGGAGAT TTAAATCCTC CTCTTCTTCC TCCTCCACCA
    TTGGCCACTC CGGTTATCAC GTCGACATCT TCAGGAGCTG CTGCCGCTGT CCAGAGGTCT
    ATCAGCGGTC CAGCTGAGCA AGTCACACAC TTGAGGACAT CTACAAGACC CAGTGTGTTT
    GTTGCTATCT ATCCCTATAT CCCACGCAAG GAGGATGAGT TGGAACTGAG GAAAGGTGAA
    ATGTTCTTGG TTTTTGAGCG CTGTCAGGAT GGCTGGTTTA AAGGAACTTC CATGCACACC
    AGCAAAATTG GTGTGTTCCC TGGAAACTAT GTTGCACCAG TCACTAGGGC ATTAACAACT
    GCAACACCAG CAAAGGTTGC CATGGCCACA GCTAGTAGCA GTAATGTGGT GAACTTAGTA
    ACGCCAACAC CTCCAGGGGC TCCATGCCAA AAACTGCAAG GGAATGGAGC GGAGTTTGCC
    AAGACTGTCA GTACAAATGG GGTCCCACCG GCTGGCATTC CTGGTTCTCA TATCCAGAGT
    AGCCCACAGG CCAAGGTTCT GCTGCACATG AGTGGCCAAA TGACCGTTAA CCAAGCCCGC
    AATGCGGTCC GAACAGCTGC TGCACACAGC CAGGATCGTC CAACAGCAGC TGTGACTCCC
    ATCCAGGCTC AGATCCCATC TGCATCTGTT CTCCCCCAGC AGGCTGCCAC TTCACAGCAA
    ATGCCACCTC CGCTTTCAGG TCCAGCTGCT TACATCAATG CTGCAATGAA CATTAGCCGA
    CCAAGTGTGC CGGTGGCTTC GGCAGCCTCC TCCAGTGTTT CCTCCGCTGC ATTTGAAACA
    GAATGTAATT GGAAATCTGG CTCTGGACTT GCTGCGTGTT CTTTTCCAGA AAATGTCTCT
    GCACCTTTAA ATTCTGCTGC TAATAAGCAA GATAAAGATA GTAAGAAAGA AAAGAAGGGC
    TTGCTCAAGT TACTGTCTGG GGCATCTACA AAAAGAAAGC CTCGCTCATC TCCGCCGCAC
    TCTCCAACCC AAGAACTGGA ACAGACAAAT TCAGAAGCAG CGCTGGAGGG AGCTGTAGGG
    CCAGACATTC TGCCTGTCAA TGGCAATGGC CGAGTTGCTT CCTGCACTGT GGATTGTGAT
    TTAGTATCTG CCTCAGCTCT GGTACAGGAC AACCGCAAGC CAGCTTCCTT GGATACCAAT
    GTTCCCATTG CACCTCCACC AAGACAGCCT TGCTCCTCAC TGGGCACTGT TCTGAATGAC
    TCCCGGCCTT GTGAAAGATA TAGAGTTGTA GTGTCCTACC CACCCCAGAG CGAGGCAGAA
    CTAGAGCTGA AGGAAGGGGA CATCGTATTT GTGCACAAGA AACGGGAAGA TGGTTGGTTT
    AAAGGCACAC TCCAACGGAA CGGCAAAACC GGGCTATTTC CCGGCAGCTT TGTGGAGAAT
    ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001015973.1
    CDS336..2921
    Translation

    Target ORF information:

    RefSeq Version NM_001015973.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis SH3 domain containing ring finger 1 (sh3rf1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001015973.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGATGAGT CAGCATTACT GGACCTCTTA GAATGTCCAG TGTGTCTTGA GAGACTTGAT 
    GCCTCTGCTA AAGTCTTGCC ATGCCAACAC ACATTCTGCA AGCGCTGCCT TCTGGGCATT
    GTGAGCTCTC GCAATGAGCT GCGCTGCCCA GAGTGCAGGA CTCTGGTGGA GTGTGGGGTG
    GATGAACTTC CGAGCAACAT CCTACTAGTG AGACTGTTGG ATGGAATAAA GCAGAGACCA
    AGAAAAGCAG GGGTTGGTGG CAGTGCTGGG AATAGCACAA ATGTGCTAAG AGCACAAGGC
    AGTCTAACTA CTAATTGTGG CTTAAATGAT GCACAGAATA TTCATGGTGG GCAACAGAGG
    ATACAGGCAC GAAGTCCTCC AGTAAGGGGT GTTCCTCAGT TACCATGTGC CAAAGCTTTG
    TACAACTACG AGGGGAAAGA GCCTGGAGAT CTGAAATTTA ACAAGGGAGA CATCATCGTG
    CTTCGCCGTC AGGTTGATGA AAATTGGTAC CACGGGGAGA TTAATGGCAT CCATGGCTTC
    TTCCCAACAA ACTTTGTGCA GATCATAAAG CCTTTACCTC AGCCTCCTCC TCAATGCAAA
    GCACTTTATG ACTTTGAAGT GAAAGACAAG GAAGCAGACA AGGATTGCCT ACCCTTTTTA
    AAGGATGACA TTCTCACCGT GATCCGACGA GTAGATGAAA ACTGGGCAGA AGGAATGCTG
    GGAGACAAGA TAGGAATATT CCCTATTTCC TATGTAGAGT TCAATTCAGC AGCCAAGCAG
    CTGATAGAAT TGGACAAGCC ATCAGGAGTA GACACTGGGG AAGGCTCTTC TGGCACAACG
    CATAGTAGCA ATTCTCAAAA GCAAGCAGAT GCAAAGAAGA ACACCAAAAA GCGTCACTCC
    TTTACCTCCC TCACCATGTC TAACAAGTCA TCGCAGTCCG TGCAGAATCG TCATTCAATG
    GAGATTAGCC CCCCTGTACT CATCAGTTCT AGCAATCCCA CCGCTGCTGC CAGGATCAGT
    GAACTCACAG GCCTCTCTTG TAGTGCCCCT TCTCAGGTTC ATATCAGTAC AACAGGGCTC
    ATTGTGACCC CACCCCCTAG CAGCCCTGTT GTAAGTGGCC CTGCATTTAC ATTTCCACCG
    GAAGTCACCT ACCAAGCTGC TCTGGGAGAT TTAAATCCTC CTCTTCTTCC TCCTCCACCA
    TTGGCCACTC CGGTTATCAC GTCGACATCT TCAGGAGCTG CTGCCGCTGT CCAGAGGTCT
    ATCAGCGGTC CAGCTGAGCA AGTCACACAC TTGAGGACAT CTACAAGACC CAGTGTGTTT
    GTTGCTATCT ATCCCTATAT CCCACGCAAG GAGGATGAGT TGGAACTGAG GAAAGGTGAA
    ATGTTCTTGG TTTTTGAGCG CTGTCAGGAT GGCTGGTTTA AAGGAACTTC CATGCACACC
    AGCAAAATTG GTGTGTTCCC TGGAAACTAT GTTGCACCAG TCACTAGGGC ATTAACAACT
    GCAACACCAG CAAAGGTTGC CATGGCCACA GCTAGTAGCA GTAATGTGGT GAACTTAGTA
    ACGCCAACAC CTCCAGGGGC TCCATGCCAA AAACTGCAAG GGAATGGAGC GGAGTTTGCC
    AAGACTGTCA GTACAAATGG GGTCCCACCG GCTGGCATTC CTGGTTCTCA TATCCAGAGT
    AGCCCACAGG CCAAGGTTCT GCTGCACATG AGTGGCCAAA TGACCGTTAA CCAAGCCCGC
    AATGCGGTCC GAACAGCTGC TGCACACAGC CAGGATCGTC CAACAGCAGC TGTGACTCCC
    ATCCAGGCTC AGATCCCATC TGCATCTGTT CTCCCCCAGC AGGCTGCCAC TTCACAGCAA
    ATGCCACCTC CGCTTTCAGG TCCAGCTGCT TACATCAATG CTGCAATGAA CATTAGCCGA
    CCAAGTGTGC CGGTGGCTTC GGCAGCCTCC TCCAGTGTTT CCTCCGCTGC ATTTGAAACA
    GAATGTAATT GGAAATCTGG CTCTGGACTT GCTGCGTGTT CTTTTCCAGA AAATGTCTCT
    GCACCTTTAA ATTCTGCTGC TAATAAGCAA GATAAAGATA GTAAGAAAGA AAAGAAGGGC
    TTGCTCAAGT TACTGTCTGG GGCATCTACA AAAAGAAAGC CTCGCTCATC TCCGCCGCAC
    TCTCCAACCC AAGAACTGGA ACAGACAAAT TCAGAAGCAG CGCTGGAGGG AGCTGTAGGG
    CCAGACATTC TGCCTGTCAA TGGCAATGGC CGAGTTGCTT CCTGCACTGT GGATTGTGAT
    TTAGTATCTG CCTCAGCTCT GGTACAGGAC AACCGCAAGC CAGCTTCCTT GGATACCAAT
    GTTCCCATTG CACCTCCACC AAGACAGCCT TGCTCCTCAC TGGGCACTGT TCTGAATGAC
    TCCCGGCCTT GTGAAAGATA TAGAGTTGTA GTGTCCTACC CACCCCAGAG CGAGGCAGAA
    CTAGAGCTGA AGGAAGGGGA CATCGTATTT GTGCACAAGA AACGGGAAGA TGGTTGGTTT
    AAAGGCACAC TCCAACGGAA CGGCAAAACC GGGCTATTTC CCGGCAGCTT TGTGGAGAAT
    ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa27509
    Clone ID Related Accession (Same CDS sequence) XM_012961866.3 , XM_012961866.2
    Accession Version XM_012961866.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2622bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase SH3RF1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961866.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTTCTTT CCATGCAAAA CTGTGTCGAT AAAACCATGG ATGAGTCAGC ATTACTGGAC 
    CTCTTAGAAT GTCCAGTGTG TCTTGAGAGA CTTGATGCCT CTGCTAAAGT CTTGCCATGC
    CAACACACAT TCTGCAAGCG CTGCCTTCTG GGCATTGTGA GCTCTCGCAA TGAGCTGCGC
    TGCCCAGAGT GCAGGACTCT GGTGGAGTGT GGGGTGGATG AACTTCCGAG CAACATCCTA
    CTAGTGAGAC TGTTGGATGG AATAAAGCAG AGACCAAGAA AAGCAGGGGT TGGTGGCAGT
    GCTGGGAATA GCACAAATGT GCTAAGAGCA CAAGGCAGTC TAACTACTAA TTGTGGCTTA
    AATGATGCAC AGAATATTCA TGGTGGGCAA CAGAGGATAC AGGCACGAAG TCCTCCAGTA
    AGGGGTGTTC CTCAGTTACC ATGTGCCAAA GCTTTGTACA ACTACGAGGG GAAAGAGCCT
    GGAGATCTGA AATTTAACAA GGGAGACATC ATCGTGCTTC GCCGTCAGGT TGATGAAAAT
    TGGTACCACG GGGAGATTAA TGGCATCCAT GGCTTCTTCC CAACAAACTT TGTGCAGATC
    ATAAAGCCTT TACCTCAGCC TCCTCCTCAA TGCAAAGCAC TTTATGACTT TGAAGTGAAA
    GACAAGGAAG CAGACAAGGA TTGCCTACCC TTTTTAAAGG ATGACATTCT CACCGTGATC
    CGACGAGTAG ATGAAAACTG GGCAGAAGGA ATGCTGGGAG ACAAGATAGG AATATTCCCT
    ATTTCCTATG TAGAGTTCAA TTCAGCAGCC AAGCAGCTGA TAGAATTGGA CAAGCCATCA
    GGAGTAGACA CTGGGGAAGG CTCTTCTGGC ACAACGCATA GTAGCAATTC TCAAAAGCAA
    GCAGATGCAA AGAAGAACAC CAAAAAGCGT CACTCCTTTA CCTCCCTCAC CATGTCTAAC
    AAGTCATCGC AGTCCGTGCA GAATCGTCAT TCAATGGAGA TTAGCCCCCC TGTACTCATC
    AGTTCTAGCA ATCCCACCGC TGCTGCCAGG ATCAGTGAAC TCACAGGCCT CTCTTGTAGT
    GCCCCTTCTC AGGTTCATAT CAGTACAACA GGGCTCATTG TGACCCCACC CCCTAGCAGC
    CCTGTTGTAA GTGGCCCTGC ATTTACATTT CCACCGGAAG TCACCTACCA AGCTGCTCTG
    GGAGATTTAA ATCCTCCTCT TCTTCCTCCT CCACCATTGG CCACTCCGGT TATCACGTCG
    ACATCTTCAG GAGCTGCTGC CGCTGTCCAG AGGTCTATCA GCGGTCCAGC TGAGCAAGTC
    ACACACTTGA GGACATCTAC AAGACCCAGT GTGTTTGTTG CTATCTATCC CTATATCCCA
    CGCAAGGAGG ATGAGTTGGA ACTGAGGAAA GGTGAAATGT TCTTGGTTTT TGAGCGCTGT
    CAGGATGGCT GGTTTAAAGG AACTTCCATG CACACCAGCA AAATTGGTGT GTTCCCTGGA
    AACTATGTTG CACCAGTCAC TAGGGCATTA ACAACTGCAA CACCAGCAAA GGTTGCCATG
    GCCACAGCTA GTAGCAGTAA TGTGGTGAAC TTAGTAACGC CAACACCTCC AGGGGCTCCA
    TGCCAAAAAC TGCAAGGGAA TGGAGCGGAG TTTGCCAAGA CTGTCAGTAC AAATGGGGTC
    CCACCGGCTG GCATTCCTGG TTCTCATATC CAGAGTAGCC CACAGGCCAA GGTTCTGCTG
    CACATGAGTG GCCAAATGAC CGTTAACCAA GCCCGCAATG CGGTCCGAAC AGCTGCTGCA
    CACAGCCAGG ATCGTCCAAC AGCAGCTGTG ACTCCCATCC AGGCTCAGAT CCCATCTGCA
    TCTGTTCTCC CCCAGCAGGC TGCCACTTCA CAGCAAATGC CACCTCCGCT TTCAGGTCCA
    GCTGCTTACA TCAATGCTGC AATGAACATT AGCCGACCAA GTGTGCCGGT GGCTTCGGCA
    GCCTCCTCCA GTGTTTCCTC CGCTGCATTT GAAACAGAAT GTAATTGGAA ATCTGGCTCT
    GGACTTGCTG CGTGTTCTTT TCCAGAAAAT GTCTCTGCAC CTTTAAATTC TGCTGCTAAT
    AAGCAAGATA AAGATAGTAA GAAAGAAAAG AAGGGCTTGC TCAAGTTACT GTCTGGGGCA
    TCTACAAAAA GAAAGCCTCG CTCATCTCCG CCGCACTCTC CAACCCAAGA ACTGGAACAG
    ACAAATTCAG AAGCAGCGCT GGAGGGAGCT GTAGGGCCAG ACATTCTGCC TGTCAATGGC
    AATGGCCGAG TTGCTTCCTG CACTGTGGAT TGTGATTTAG TATCTGCCTC AGCTCTGGTA
    CAGGACAACC GCAAGCCAGC TTCCTTGGAT ACCAATGTTC CCATTGCACC TCCACCAAGA
    CAGCCTTGCT CCTCACTGGG CACTGTTCTG AATGACTCCC GGCCTTGTGA AAGATATAGA
    GTTGTAGTGT CCTACCCACC CCAGAGCGAG GCAGAACTAG AGCTGAAGGA AGGGGACATC
    GTATTTGTGC ACAAGAAACG GGAAGATGGT TGGTTTAAAG GCACACTCCA ACGGAACGGC
    AAAACCGGGC TATTTCCCGG CAGCTTTGTG GAGAATATTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817320.1
    CDS582..3203
    Translation

    Target ORF information:

    RefSeq Version XM_012961866.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis SH3 domain containing ring finger 1 (sh3rf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961866.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTTCTTT CCATGCAAAA CTGTGTCGAT AAAACCATGG ATGAGTCAGC ATTACTGGAC 
    CTCTTAGAAT GTCCAGTGTG TCTTGAGAGA CTTGATGCCT CTGCTAAAGT CTTGCCATGC
    CAACACACAT TCTGCAAGCG CTGCCTTCTG GGCATTGTGA GCTCTCGCAA TGAGCTGCGC
    TGCCCAGAGT GCAGGACTCT GGTGGAGTGT GGGGTGGATG AACTTCCGAG CAACATCCTA
    CTAGTGAGAC TGTTGGATGG AATAAAGCAG AGACCAAGAA AAGCAGGGGT TGGTGGCAGT
    GCTGGGAATA GCACAAATGT GCTAAGAGCA CAAGGCAGTC TAACTACTAA TTGTGGCTTA
    AATGATGCAC AGAATATTCA TGGTGGGCAA CAGAGGATAC AGGCACGAAG TCCTCCAGTA
    AGGGGTGTTC CTCAGTTACC ATGTGCCAAA GCTTTGTACA ACTACGAGGG GAAAGAGCCT
    GGAGATCTGA AATTTAACAA GGGAGACATC ATCGTGCTTC GCCGTCAGGT TGATGAAAAT
    TGGTACCACG GGGAGATTAA TGGCATCCAT GGCTTCTTCC CAACAAACTT TGTGCAGATC
    ATAAAGCCTT TACCTCAGCC TCCTCCTCAA TGCAAAGCAC TTTATGACTT TGAAGTGAAA
    GACAAGGAAG CAGACAAGGA TTGCCTACCC TTTTTAAAGG ATGACATTCT CACCGTGATC
    CGACGAGTAG ATGAAAACTG GGCAGAAGGA ATGCTGGGAG ACAAGATAGG AATATTCCCT
    ATTTCCTATG TAGAGTTCAA TTCAGCAGCC AAGCAGCTGA TAGAATTGGA CAAGCCATCA
    GGAGTAGACA CTGGGGAAGG CTCTTCTGGC ACAACGCATA GTAGCAATTC TCAAAAGCAA
    GCAGATGCAA AGAAGAACAC CAAAAAGCGT CACTCCTTTA CCTCCCTCAC CATGTCTAAC
    AAGTCATCGC AGTCCGTGCA GAATCGTCAT TCAATGGAGA TTAGCCCCCC TGTACTCATC
    AGTTCTAGCA ATCCCACCGC TGCTGCCAGG ATCAGTGAAC TCACAGGCCT CTCTTGTAGT
    GCCCCTTCTC AGGTTCATAT CAGTACAACA GGGCTCATTG TGACCCCACC CCCTAGCAGC
    CCTGTTGTAA GTGGCCCTGC ATTTACATTT CCACCGGAAG TCACCTACCA AGCTGCTCTG
    GGAGATTTAA ATCCTCCTCT TCTTCCTCCT CCACCATTGG CCACTCCGGT TATCACGTCG
    ACATCTTCAG GAGCTGCTGC CGCTGTCCAG AGGTCTATCA GCGGTCCAGC TGAGCAAGTC
    ACACACTTGA GGACATCTAC AAGACCCAGT GTGTTTGTTG CTATCTATCC CTATATCCCA
    CGCAAGGAGG ATGAGTTGGA ACTGAGGAAA GGTGAAATGT TCTTGGTTTT TGAGCGCTGT
    CAGGATGGCT GGTTTAAAGG AACTTCCATG CACACCAGCA AAATTGGTGT GTTCCCTGGA
    AACTATGTTG CACCAGTCAC TAGGGCATTA ACAACTGCAA CACCAGCAAA GGTTGCCATG
    GCCACAGCTA GTAGCAGTAA TGTGGTGAAC TTAGTAACGC CAACACCTCC AGGGGCTCCA
    TGCCAAAAAC TGCAAGGGAA TGGAGCGGAG TTTGCCAAGA CTGTCAGTAC AAATGGGGTC
    CCACCGGCTG GCATTCCTGG TTCTCATATC CAGAGTAGCC CACAGGCCAA GGTTCTGCTG
    CACATGAGTG GCCAAATGAC CGTTAACCAA GCCCGCAATG CGGTCCGAAC AGCTGCTGCA
    CACAGCCAGG ATCGTCCAAC AGCAGCTGTG ACTCCCATCC AGGCTCAGAT CCCATCTGCA
    TCTGTTCTCC CCCAGCAGGC TGCCACTTCA CAGCAAATGC CACCTCCGCT TTCAGGTCCA
    GCTGCTTACA TCAATGCTGC AATGAACATT AGCCGACCAA GTGTGCCGGT GGCTTCGGCA
    GCCTCCTCCA GTGTTTCCTC CGCTGCATTT GAAACAGAAT GTAATTGGAA ATCTGGCTCT
    GGACTTGCTG CGTGTTCTTT TCCAGAAAAT GTCTCTGCAC CTTTAAATTC TGCTGCTAAT
    AAGCAAGATA AAGATAGTAA GAAAGAAAAG AAGGGCTTGC TCAAGTTACT GTCTGGGGCA
    TCTACAAAAA GAAAGCCTCG CTCATCTCCG CCGCACTCTC CAACCCAAGA ACTGGAACAG
    ACAAATTCAG AAGCAGCGCT GGAGGGAGCT GTAGGGCCAG ACATTCTGCC TGTCAATGGC
    AATGGCCGAG TTGCTTCCTG CACTGTGGAT TGTGATTTAG TATCTGCCTC AGCTCTGGTA
    CAGGACAACC GCAAGCCAGC TTCCTTGGAT ACCAATGTTC CCATTGCACC TCCACCAAGA
    CAGCCTTGCT CCTCACTGGG CACTGTTCTG AATGACTCCC GGCCTTGTGA AAGATATAGA
    GTTGTAGTGT CCTACCCACC CCAGAGCGAG GCAGAACTAG AGCTGAAGGA AGGGGACATC
    GTATTTGTGC ACAAGAAACG GGAAGATGGT TGGTTTAAAG GCACACTCCA ACGGAACGGC
    AAAACCGGGC TATTTCCCGG CAGCTTTGTG GAGAATATTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa27510
    Clone ID Related Accession (Same CDS sequence) XM_012961871.2 , XM_012961878.3 , XM_012961878.2 , XM_012961871.3
    Accession Version XM_012961871.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase SH3RF1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961871.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTTCTTT CCATGCAAAA CTGTGTCGAT AAAACCATGG ATGAGTCAGC ATTACTGGAC 
    CTCTTAGAAT GTCCAGTGTG TCTTGAGAGA CTTGATGCCT CTGCTAAAGT CTTGCCATGC
    CAACACACAT TCTGCAAGCG CTGCCTTCTG GGCATTGTGA GCTCTCGCAA TGAGCTGCGC
    TGCCCAGAGT GCAGGACTCT GGTGGAGTGT GGGGTGGATG AACTTCCGAG CAACATCCTA
    CTAGTGAGAC TGTTGGATGG AATAAAGCAG AGACCAAGAA AAGCAGGGGT TGGTGGCAGT
    GCTGGGAATA GCACAAATGT GCTAAGAGCA CAAGGCAGTC TAACTACTAA TTGTGGCTTA
    AATGATGCAC AGAATATTCA TGGTGGGCAA CAGAGGATAC AGGCACGAAG TCCTCCAGTA
    AGGGGTGTTC CTCAGTTACC ATGTGCCAAA GCTTTGTACA ACTACGAGGG GAAAGAGCCT
    GGAGATCTGA AATTTAACAA GGGAGACATC ATCGTGCTTC GCCGTCAGGT TGATGAAAAT
    TGGTACCACG GGGAGATTAA TGGCATCCAT GGCTTCTTCC CAACAAACTT TGTGCAGATC
    ATAAAGCCTT TACCTCAGCC TCCTCCTCAA TGCAAAGCAC TTTATGACTT TGAAGTGAAA
    GACAAGGAAG CAGACAAGGA TTGCCTACCC TTTTTAAAGG ATGACATTCT CACCGTGATC
    CGACGAGTAG ATGAAAACTG GGCAGAAGGA ATGCTGGGAG ACAAGATAGG AATATTCCCT
    ATTTCCTATG TAGAGTTCAA TTCAGCAGCC AAGCAGCTGA TAGAATTGGA CAAGCCATCA
    GGAGTAGACA CTGGGGAAGG CTCTTCTGGC ACAACGCATA GTAGCAATTC TCAAAAGCAA
    GCAGATGCAA AGAAGAACAC CAAAAAGCGT CACTCCTTTA CCTCCCTCAC CATGTCTAAC
    AAGTCATCGC AGTCCGTGCA GAATCGTCAT TCAATGGAGA TTAGCCCCCC TGTACTCATC
    AGTTCTAGCA ATCCCACCGC TGCTGCCAGG ATCAGTGAAC TCACAGGCCT CTCTTGTAGT
    GCCCCTTCTC AGGATTTAAA TCCTCCTCTT CTTCCTCCTC CACCATTGGC CACTCCGGTT
    ATCACGTCGA CATCTTCAGG AGCTGCTGCC GCTGTCCAGA GGTCTATCAG CGGTCCAGCT
    GAGCAAGTCA CACACTTGAG GACATCTACA AGACCCAGTG TGTTTGTTGC TATCTATCCC
    TATATCCCAC GCAAGGAGGA TGAGTTGGAA CTGAGGAAAG GTGAAATGTT CTTGGTTTTT
    GAGCGCTGTC AGGATGGCTG GTTTAAAGGA ACTTCCATGC ACACCAGCAA AATTGGTGTG
    TTCCCTGGAA ACTATGTTGC ACCAGTCACT AGGGCATTAA CAACTGCAAC ACCAGCAAAG
    GTTGCCATGG CCACAGCTAG TAGCAGTAAT GTGGTGAACT TAGTAACGCC AACACCTCCA
    GGGGCTCCAT GCCAAAAACT GCAAGGGAAT GGAGCGGAGT TTGCCAAGAC TGTCAGTACA
    AATGGGGTCC CACCGGCTGG CATTCCTGGT TCTCATATCC AGAGTAGCCC ACAGGCCAAG
    GTTCTGCTGC ACATGAGTGG CCAAATGACC GTTAACCAAG CCCGCAATGC GGTCCGAACA
    GCTGCTGCAC ACAGCCAGGA TCGTCCAACA GCAGCTGTGA CTCCCATCCA GGCTCAGATC
    CCATCTGCAT CTGTTCTCCC CCAGCAGGCT GCCACTTCAC AGCAAATGCC ACCTCCGCTT
    TCAGGTCCAG CTGCTTACAT CAATGCTGCA ATGAACATTA GCCGACCAAG TGTGCCGGTG
    GCTTCGGCAG CCTCCTCCAG TGTTTCCTCC GCTGCATTTG AAACAGAATG TAATTGGAAA
    TCTGGCTCTG GACTTGCTGC GTGTTCTTTT CCAGAAAATG TCTCTGCACC TTTAAATTCT
    GCTGCTAATA AGCAAGATAA AGATAGTAAG AAAGAAAAGA AGGGCTTGCT CAAGTTACTG
    TCTGGGGCAT CTACAAAAAG AAAGCCTCGC TCATCTCCGC CGCACTCTCC AACCCAAGAA
    CTGGAACAGA CAAATTCAGA AGCAGCGCTG GAGGGAGCTG TAGGGCCAGA CATTCTGCCT
    GTCAATGGCA ATGGCCGAGT TGCTTCCTGC ACTGTGGATT GTGATTTAGT ATCTGCCTCA
    GCTCTGGTAC AGGACAACCG CAAGCCAGCT TCCTTGGATA CCAATGTTCC CATTGCACCT
    CCACCAAGAC AGCCTTGCTC CTCACTGGGC ACTGTTCTGA ATGACTCCCG GCCTTGTGAA
    AGATATAGAG TTGTAGTGTC CTACCCACCC CAGAGCGAGG CAGAACTAGA GCTGAAGGAA
    GGGGACATCG TATTTGTGCA CAAGAAACGG GAAGATGGTT GGTTTAAAGG CACACTCCAA
    CGGAACGGCA AAACCGGGCT ATTTCCCGGC AGCTTTGTGG AGAATATTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817325.1
    CDS611..3121
    Translation

    Target ORF information:

    RefSeq Version XM_012961871.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis SH3 domain containing ring finger 1 (sh3rf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961871.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTTCTTT CCATGCAAAA CTGTGTCGAT AAAACCATGG ATGAGTCAGC ATTACTGGAC 
    CTCTTAGAAT GTCCAGTGTG TCTTGAGAGA CTTGATGCCT CTGCTAAAGT CTTGCCATGC
    CAACACACAT TCTGCAAGCG CTGCCTTCTG GGCATTGTGA GCTCTCGCAA TGAGCTGCGC
    TGCCCAGAGT GCAGGACTCT GGTGGAGTGT GGGGTGGATG AACTTCCGAG CAACATCCTA
    CTAGTGAGAC TGTTGGATGG AATAAAGCAG AGACCAAGAA AAGCAGGGGT TGGTGGCAGT
    GCTGGGAATA GCACAAATGT GCTAAGAGCA CAAGGCAGTC TAACTACTAA TTGTGGCTTA
    AATGATGCAC AGAATATTCA TGGTGGGCAA CAGAGGATAC AGGCACGAAG TCCTCCAGTA
    AGGGGTGTTC CTCAGTTACC ATGTGCCAAA GCTTTGTACA ACTACGAGGG GAAAGAGCCT
    GGAGATCTGA AATTTAACAA GGGAGACATC ATCGTGCTTC GCCGTCAGGT TGATGAAAAT
    TGGTACCACG GGGAGATTAA TGGCATCCAT GGCTTCTTCC CAACAAACTT TGTGCAGATC
    ATAAAGCCTT TACCTCAGCC TCCTCCTCAA TGCAAAGCAC TTTATGACTT TGAAGTGAAA
    GACAAGGAAG CAGACAAGGA TTGCCTACCC TTTTTAAAGG ATGACATTCT CACCGTGATC
    CGACGAGTAG ATGAAAACTG GGCAGAAGGA ATGCTGGGAG ACAAGATAGG AATATTCCCT
    ATTTCCTATG TAGAGTTCAA TTCAGCAGCC AAGCAGCTGA TAGAATTGGA CAAGCCATCA
    GGAGTAGACA CTGGGGAAGG CTCTTCTGGC ACAACGCATA GTAGCAATTC TCAAAAGCAA
    GCAGATGCAA AGAAGAACAC CAAAAAGCGT CACTCCTTTA CCTCCCTCAC CATGTCTAAC
    AAGTCATCGC AGTCCGTGCA GAATCGTCAT TCAATGGAGA TTAGCCCCCC TGTACTCATC
    AGTTCTAGCA ATCCCACCGC TGCTGCCAGG ATCAGTGAAC TCACAGGCCT CTCTTGTAGT
    GCCCCTTCTC AGGATTTAAA TCCTCCTCTT CTTCCTCCTC CACCATTGGC CACTCCGGTT
    ATCACGTCGA CATCTTCAGG AGCTGCTGCC GCTGTCCAGA GGTCTATCAG CGGTCCAGCT
    GAGCAAGTCA CACACTTGAG GACATCTACA AGACCCAGTG TGTTTGTTGC TATCTATCCC
    TATATCCCAC GCAAGGAGGA TGAGTTGGAA CTGAGGAAAG GTGAAATGTT CTTGGTTTTT
    GAGCGCTGTC AGGATGGCTG GTTTAAAGGA ACTTCCATGC ACACCAGCAA AATTGGTGTG
    TTCCCTGGAA ACTATGTTGC ACCAGTCACT AGGGCATTAA CAACTGCAAC ACCAGCAAAG
    GTTGCCATGG CCACAGCTAG TAGCAGTAAT GTGGTGAACT TAGTAACGCC AACACCTCCA
    GGGGCTCCAT GCCAAAAACT GCAAGGGAAT GGAGCGGAGT TTGCCAAGAC TGTCAGTACA
    AATGGGGTCC CACCGGCTGG CATTCCTGGT TCTCATATCC AGAGTAGCCC ACAGGCCAAG
    GTTCTGCTGC ACATGAGTGG CCAAATGACC GTTAACCAAG CCCGCAATGC GGTCCGAACA
    GCTGCTGCAC ACAGCCAGGA TCGTCCAACA GCAGCTGTGA CTCCCATCCA GGCTCAGATC
    CCATCTGCAT CTGTTCTCCC CCAGCAGGCT GCCACTTCAC AGCAAATGCC ACCTCCGCTT
    TCAGGTCCAG CTGCTTACAT CAATGCTGCA ATGAACATTA GCCGACCAAG TGTGCCGGTG
    GCTTCGGCAG CCTCCTCCAG TGTTTCCTCC GCTGCATTTG AAACAGAATG TAATTGGAAA
    TCTGGCTCTG GACTTGCTGC GTGTTCTTTT CCAGAAAATG TCTCTGCACC TTTAAATTCT
    GCTGCTAATA AGCAAGATAA AGATAGTAAG AAAGAAAAGA AGGGCTTGCT CAAGTTACTG
    TCTGGGGCAT CTACAAAAAG AAAGCCTCGC TCATCTCCGC CGCACTCTCC AACCCAAGAA
    CTGGAACAGA CAAATTCAGA AGCAGCGCTG GAGGGAGCTG TAGGGCCAGA CATTCTGCCT
    GTCAATGGCA ATGGCCGAGT TGCTTCCTGC ACTGTGGATT GTGATTTAGT ATCTGCCTCA
    GCTCTGGTAC AGGACAACCG CAAGCCAGCT TCCTTGGATA CCAATGTTCC CATTGCACCT
    CCACCAAGAC AGCCTTGCTC CTCACTGGGC ACTGTTCTGA ATGACTCCCG GCCTTGTGAA
    AGATATAGAG TTGTAGTGTC CTACCCACCC CAGAGCGAGG CAGAACTAGA GCTGAAGGAA
    GGGGACATCG TATTTGTGCA CAAGAAACGG GAAGATGGTT GGTTTAAAGG CACACTCCAA
    CGGAACGGCA AAACCGGGCT ATTTCCCGGC AGCTTTGTGG AGAATATTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa27510
    Clone ID Related Accession (Same CDS sequence) XM_012961871.2 , XM_012961878.3 , XM_012961878.2 , XM_012961871.3
    Accession Version XM_012961878.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase SH3RF1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961878.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTTCTTT CCATGCAAAA CTGTGTCGAT AAAACCATGG ATGAGTCAGC ATTACTGGAC 
    CTCTTAGAAT GTCCAGTGTG TCTTGAGAGA CTTGATGCCT CTGCTAAAGT CTTGCCATGC
    CAACACACAT TCTGCAAGCG CTGCCTTCTG GGCATTGTGA GCTCTCGCAA TGAGCTGCGC
    TGCCCAGAGT GCAGGACTCT GGTGGAGTGT GGGGTGGATG AACTTCCGAG CAACATCCTA
    CTAGTGAGAC TGTTGGATGG AATAAAGCAG AGACCAAGAA AAGCAGGGGT TGGTGGCAGT
    GCTGGGAATA GCACAAATGT GCTAAGAGCA CAAGGCAGTC TAACTACTAA TTGTGGCTTA
    AATGATGCAC AGAATATTCA TGGTGGGCAA CAGAGGATAC AGGCACGAAG TCCTCCAGTA
    AGGGGTGTTC CTCAGTTACC ATGTGCCAAA GCTTTGTACA ACTACGAGGG GAAAGAGCCT
    GGAGATCTGA AATTTAACAA GGGAGACATC ATCGTGCTTC GCCGTCAGGT TGATGAAAAT
    TGGTACCACG GGGAGATTAA TGGCATCCAT GGCTTCTTCC CAACAAACTT TGTGCAGATC
    ATAAAGCCTT TACCTCAGCC TCCTCCTCAA TGCAAAGCAC TTTATGACTT TGAAGTGAAA
    GACAAGGAAG CAGACAAGGA TTGCCTACCC TTTTTAAAGG ATGACATTCT CACCGTGATC
    CGACGAGTAG ATGAAAACTG GGCAGAAGGA ATGCTGGGAG ACAAGATAGG AATATTCCCT
    ATTTCCTATG TAGAGTTCAA TTCAGCAGCC AAGCAGCTGA TAGAATTGGA CAAGCCATCA
    GGAGTAGACA CTGGGGAAGG CTCTTCTGGC ACAACGCATA GTAGCAATTC TCAAAAGCAA
    GCAGATGCAA AGAAGAACAC CAAAAAGCGT CACTCCTTTA CCTCCCTCAC CATGTCTAAC
    AAGTCATCGC AGTCCGTGCA GAATCGTCAT TCAATGGAGA TTAGCCCCCC TGTACTCATC
    AGTTCTAGCA ATCCCACCGC TGCTGCCAGG ATCAGTGAAC TCACAGGCCT CTCTTGTAGT
    GCCCCTTCTC AGGATTTAAA TCCTCCTCTT CTTCCTCCTC CACCATTGGC CACTCCGGTT
    ATCACGTCGA CATCTTCAGG AGCTGCTGCC GCTGTCCAGA GGTCTATCAG CGGTCCAGCT
    GAGCAAGTCA CACACTTGAG GACATCTACA AGACCCAGTG TGTTTGTTGC TATCTATCCC
    TATATCCCAC GCAAGGAGGA TGAGTTGGAA CTGAGGAAAG GTGAAATGTT CTTGGTTTTT
    GAGCGCTGTC AGGATGGCTG GTTTAAAGGA ACTTCCATGC ACACCAGCAA AATTGGTGTG
    TTCCCTGGAA ACTATGTTGC ACCAGTCACT AGGGCATTAA CAACTGCAAC ACCAGCAAAG
    GTTGCCATGG CCACAGCTAG TAGCAGTAAT GTGGTGAACT TAGTAACGCC AACACCTCCA
    GGGGCTCCAT GCCAAAAACT GCAAGGGAAT GGAGCGGAGT TTGCCAAGAC TGTCAGTACA
    AATGGGGTCC CACCGGCTGG CATTCCTGGT TCTCATATCC AGAGTAGCCC ACAGGCCAAG
    GTTCTGCTGC ACATGAGTGG CCAAATGACC GTTAACCAAG CCCGCAATGC GGTCCGAACA
    GCTGCTGCAC ACAGCCAGGA TCGTCCAACA GCAGCTGTGA CTCCCATCCA GGCTCAGATC
    CCATCTGCAT CTGTTCTCCC CCAGCAGGCT GCCACTTCAC AGCAAATGCC ACCTCCGCTT
    TCAGGTCCAG CTGCTTACAT CAATGCTGCA ATGAACATTA GCCGACCAAG TGTGCCGGTG
    GCTTCGGCAG CCTCCTCCAG TGTTTCCTCC GCTGCATTTG AAACAGAATG TAATTGGAAA
    TCTGGCTCTG GACTTGCTGC GTGTTCTTTT CCAGAAAATG TCTCTGCACC TTTAAATTCT
    GCTGCTAATA AGCAAGATAA AGATAGTAAG AAAGAAAAGA AGGGCTTGCT CAAGTTACTG
    TCTGGGGCAT CTACAAAAAG AAAGCCTCGC TCATCTCCGC CGCACTCTCC AACCCAAGAA
    CTGGAACAGA CAAATTCAGA AGCAGCGCTG GAGGGAGCTG TAGGGCCAGA CATTCTGCCT
    GTCAATGGCA ATGGCCGAGT TGCTTCCTGC ACTGTGGATT GTGATTTAGT ATCTGCCTCA
    GCTCTGGTAC AGGACAACCG CAAGCCAGCT TCCTTGGATA CCAATGTTCC CATTGCACCT
    CCACCAAGAC AGCCTTGCTC CTCACTGGGC ACTGTTCTGA ATGACTCCCG GCCTTGTGAA
    AGATATAGAG TTGTAGTGTC CTACCCACCC CAGAGCGAGG CAGAACTAGA GCTGAAGGAA
    GGGGACATCG TATTTGTGCA CAAGAAACGG GAAGATGGTT GGTTTAAAGG CACACTCCAA
    CGGAACGGCA AAACCGGGCT ATTTCCCGGC AGCTTTGTGG AGAATATTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817332.1
    CDS580..3090
    Translation

    Target ORF information:

    RefSeq Version XM_012961878.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis SH3 domain containing ring finger 1 (sh3rf1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961878.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTTCTTT CCATGCAAAA CTGTGTCGAT AAAACCATGG ATGAGTCAGC ATTACTGGAC 
    CTCTTAGAAT GTCCAGTGTG TCTTGAGAGA CTTGATGCCT CTGCTAAAGT CTTGCCATGC
    CAACACACAT TCTGCAAGCG CTGCCTTCTG GGCATTGTGA GCTCTCGCAA TGAGCTGCGC
    TGCCCAGAGT GCAGGACTCT GGTGGAGTGT GGGGTGGATG AACTTCCGAG CAACATCCTA
    CTAGTGAGAC TGTTGGATGG AATAAAGCAG AGACCAAGAA AAGCAGGGGT TGGTGGCAGT
    GCTGGGAATA GCACAAATGT GCTAAGAGCA CAAGGCAGTC TAACTACTAA TTGTGGCTTA
    AATGATGCAC AGAATATTCA TGGTGGGCAA CAGAGGATAC AGGCACGAAG TCCTCCAGTA
    AGGGGTGTTC CTCAGTTACC ATGTGCCAAA GCTTTGTACA ACTACGAGGG GAAAGAGCCT
    GGAGATCTGA AATTTAACAA GGGAGACATC ATCGTGCTTC GCCGTCAGGT TGATGAAAAT
    TGGTACCACG GGGAGATTAA TGGCATCCAT GGCTTCTTCC CAACAAACTT TGTGCAGATC
    ATAAAGCCTT TACCTCAGCC TCCTCCTCAA TGCAAAGCAC TTTATGACTT TGAAGTGAAA
    GACAAGGAAG CAGACAAGGA TTGCCTACCC TTTTTAAAGG ATGACATTCT CACCGTGATC
    CGACGAGTAG ATGAAAACTG GGCAGAAGGA ATGCTGGGAG ACAAGATAGG AATATTCCCT
    ATTTCCTATG TAGAGTTCAA TTCAGCAGCC AAGCAGCTGA TAGAATTGGA CAAGCCATCA
    GGAGTAGACA CTGGGGAAGG CTCTTCTGGC ACAACGCATA GTAGCAATTC TCAAAAGCAA
    GCAGATGCAA AGAAGAACAC CAAAAAGCGT CACTCCTTTA CCTCCCTCAC CATGTCTAAC
    AAGTCATCGC AGTCCGTGCA GAATCGTCAT TCAATGGAGA TTAGCCCCCC TGTACTCATC
    AGTTCTAGCA ATCCCACCGC TGCTGCCAGG ATCAGTGAAC TCACAGGCCT CTCTTGTAGT
    GCCCCTTCTC AGGATTTAAA TCCTCCTCTT CTTCCTCCTC CACCATTGGC CACTCCGGTT
    ATCACGTCGA CATCTTCAGG AGCTGCTGCC GCTGTCCAGA GGTCTATCAG CGGTCCAGCT
    GAGCAAGTCA CACACTTGAG GACATCTACA AGACCCAGTG TGTTTGTTGC TATCTATCCC
    TATATCCCAC GCAAGGAGGA TGAGTTGGAA CTGAGGAAAG GTGAAATGTT CTTGGTTTTT
    GAGCGCTGTC AGGATGGCTG GTTTAAAGGA ACTTCCATGC ACACCAGCAA AATTGGTGTG
    TTCCCTGGAA ACTATGTTGC ACCAGTCACT AGGGCATTAA CAACTGCAAC ACCAGCAAAG
    GTTGCCATGG CCACAGCTAG TAGCAGTAAT GTGGTGAACT TAGTAACGCC AACACCTCCA
    GGGGCTCCAT GCCAAAAACT GCAAGGGAAT GGAGCGGAGT TTGCCAAGAC TGTCAGTACA
    AATGGGGTCC CACCGGCTGG CATTCCTGGT TCTCATATCC AGAGTAGCCC ACAGGCCAAG
    GTTCTGCTGC ACATGAGTGG CCAAATGACC GTTAACCAAG CCCGCAATGC GGTCCGAACA
    GCTGCTGCAC ACAGCCAGGA TCGTCCAACA GCAGCTGTGA CTCCCATCCA GGCTCAGATC
    CCATCTGCAT CTGTTCTCCC CCAGCAGGCT GCCACTTCAC AGCAAATGCC ACCTCCGCTT
    TCAGGTCCAG CTGCTTACAT CAATGCTGCA ATGAACATTA GCCGACCAAG TGTGCCGGTG
    GCTTCGGCAG CCTCCTCCAG TGTTTCCTCC GCTGCATTTG AAACAGAATG TAATTGGAAA
    TCTGGCTCTG GACTTGCTGC GTGTTCTTTT CCAGAAAATG TCTCTGCACC TTTAAATTCT
    GCTGCTAATA AGCAAGATAA AGATAGTAAG AAAGAAAAGA AGGGCTTGCT CAAGTTACTG
    TCTGGGGCAT CTACAAAAAG AAAGCCTCGC TCATCTCCGC CGCACTCTCC AACCCAAGAA
    CTGGAACAGA CAAATTCAGA AGCAGCGCTG GAGGGAGCTG TAGGGCCAGA CATTCTGCCT
    GTCAATGGCA ATGGCCGAGT TGCTTCCTGC ACTGTGGATT GTGATTTAGT ATCTGCCTCA
    GCTCTGGTAC AGGACAACCG CAAGCCAGCT TCCTTGGATA CCAATGTTCC CATTGCACCT
    CCACCAAGAC AGCCTTGCTC CTCACTGGGC ACTGTTCTGA ATGACTCCCG GCCTTGTGAA
    AGATATAGAG TTGTAGTGTC CTACCCACCC CAGAGCGAGG CAGAACTAGA GCTGAAGGAA
    GGGGACATCG TATTTGTGCA CAAGAAACGG GAAGATGGTT GGTTTAAAGG CACACTCCAA
    CGGAACGGCA AAACCGGGCT ATTTCCCGGC AGCTTTGTGG AGAATATTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa27509
    Clone ID Related Accession (Same CDS sequence) XM_012961866.3 , XM_012961866.2
    Accession Version XM_012961866.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2622bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase SH3RF1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961866.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTTCTTT CCATGCAAAA CTGTGTCGAT AAAACCATGG ATGAGTCAGC ATTACTGGAC 
    CTCTTAGAAT GTCCAGTGTG TCTTGAGAGA CTTGATGCCT CTGCTAAAGT CTTGCCATGC
    CAACACACAT TCTGCAAGCG CTGCCTTCTG GGCATTGTGA GCTCTCGCAA TGAGCTGCGC
    TGCCCAGAGT GCAGGACTCT GGTGGAGTGT GGGGTGGATG AACTTCCGAG CAACATCCTA
    CTAGTGAGAC TGTTGGATGG AATAAAGCAG AGACCAAGAA AAGCAGGGGT TGGTGGCAGT
    GCTGGGAATA GCACAAATGT GCTAAGAGCA CAAGGCAGTC TAACTACTAA TTGTGGCTTA
    AATGATGCAC AGAATATTCA TGGTGGGCAA CAGAGGATAC AGGCACGAAG TCCTCCAGTA
    AGGGGTGTTC CTCAGTTACC ATGTGCCAAA GCTTTGTACA ACTACGAGGG GAAAGAGCCT
    GGAGATCTGA AATTTAACAA GGGAGACATC ATCGTGCTTC GCCGTCAGGT TGATGAAAAT
    TGGTACCACG GGGAGATTAA TGGCATCCAT GGCTTCTTCC CAACAAACTT TGTGCAGATC
    ATAAAGCCTT TACCTCAGCC TCCTCCTCAA TGCAAAGCAC TTTATGACTT TGAAGTGAAA
    GACAAGGAAG CAGACAAGGA TTGCCTACCC TTTTTAAAGG ATGACATTCT CACCGTGATC
    CGACGAGTAG ATGAAAACTG GGCAGAAGGA ATGCTGGGAG ACAAGATAGG AATATTCCCT
    ATTTCCTATG TAGAGTTCAA TTCAGCAGCC AAGCAGCTGA TAGAATTGGA CAAGCCATCA
    GGAGTAGACA CTGGGGAAGG CTCTTCTGGC ACAACGCATA GTAGCAATTC TCAAAAGCAA
    GCAGATGCAA AGAAGAACAC CAAAAAGCGT CACTCCTTTA CCTCCCTCAC CATGTCTAAC
    AAGTCATCGC AGTCCGTGCA GAATCGTCAT TCAATGGAGA TTAGCCCCCC TGTACTCATC
    AGTTCTAGCA ATCCCACCGC TGCTGCCAGG ATCAGTGAAC TCACAGGCCT CTCTTGTAGT
    GCCCCTTCTC AGGTTCATAT CAGTACAACA GGGCTCATTG TGACCCCACC CCCTAGCAGC
    CCTGTTGTAA GTGGCCCTGC ATTTACATTT CCACCGGAAG TCACCTACCA AGCTGCTCTG
    GGAGATTTAA ATCCTCCTCT TCTTCCTCCT CCACCATTGG CCACTCCGGT TATCACGTCG
    ACATCTTCAG GAGCTGCTGC CGCTGTCCAG AGGTCTATCA GCGGTCCAGC TGAGCAAGTC
    ACACACTTGA GGACATCTAC AAGACCCAGT GTGTTTGTTG CTATCTATCC CTATATCCCA
    CGCAAGGAGG ATGAGTTGGA ACTGAGGAAA GGTGAAATGT TCTTGGTTTT TGAGCGCTGT
    CAGGATGGCT GGTTTAAAGG AACTTCCATG CACACCAGCA AAATTGGTGT GTTCCCTGGA
    AACTATGTTG CACCAGTCAC TAGGGCATTA ACAACTGCAA CACCAGCAAA GGTTGCCATG
    GCCACAGCTA GTAGCAGTAA TGTGGTGAAC TTAGTAACGC CAACACCTCC AGGGGCTCCA
    TGCCAAAAAC TGCAAGGGAA TGGAGCGGAG TTTGCCAAGA CTGTCAGTAC AAATGGGGTC
    CCACCGGCTG GCATTCCTGG TTCTCATATC CAGAGTAGCC CACAGGCCAA GGTTCTGCTG
    CACATGAGTG GCCAAATGAC CGTTAACCAA GCCCGCAATG CGGTCCGAAC AGCTGCTGCA
    CACAGCCAGG ATCGTCCAAC AGCAGCTGTG ACTCCCATCC AGGCTCAGAT CCCATCTGCA
    TCTGTTCTCC CCCAGCAGGC TGCCACTTCA CAGCAAATGC CACCTCCGCT TTCAGGTCCA
    GCTGCTTACA TCAATGCTGC AATGAACATT AGCCGACCAA GTGTGCCGGT GGCTTCGGCA
    GCCTCCTCCA GTGTTTCCTC CGCTGCATTT GAAACAGAAT GTAATTGGAA ATCTGGCTCT
    GGACTTGCTG CGTGTTCTTT TCCAGAAAAT GTCTCTGCAC CTTTAAATTC TGCTGCTAAT
    AAGCAAGATA AAGATAGTAA GAAAGAAAAG AAGGGCTTGC TCAAGTTACT GTCTGGGGCA
    TCTACAAAAA GAAAGCCTCG CTCATCTCCG CCGCACTCTC CAACCCAAGA ACTGGAACAG
    ACAAATTCAG AAGCAGCGCT GGAGGGAGCT GTAGGGCCAG ACATTCTGCC TGTCAATGGC
    AATGGCCGAG TTGCTTCCTG CACTGTGGAT TGTGATTTAG TATCTGCCTC AGCTCTGGTA
    CAGGACAACC GCAAGCCAGC TTCCTTGGAT ACCAATGTTC CCATTGCACC TCCACCAAGA
    CAGCCTTGCT CCTCACTGGG CACTGTTCTG AATGACTCCC GGCCTTGTGA AAGATATAGA
    GTTGTAGTGT CCTACCCACC CCAGAGCGAG GCAGAACTAG AGCTGAAGGA AGGGGACATC
    GTATTTGTGC ACAAGAAACG GGAAGATGGT TGGTTTAAAG GCACACTCCA ACGGAACGGC
    AAAACCGGGC TATTTCCCGG CAGCTTTGTG GAGAATATTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817320.1
    CDS392..3013
    Translation

    Target ORF information:

    RefSeq Version XM_012961866.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis SH3 domain containing ring finger 1 (sh3rf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961866.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTTCTTT CCATGCAAAA CTGTGTCGAT AAAACCATGG ATGAGTCAGC ATTACTGGAC 
    CTCTTAGAAT GTCCAGTGTG TCTTGAGAGA CTTGATGCCT CTGCTAAAGT CTTGCCATGC
    CAACACACAT TCTGCAAGCG CTGCCTTCTG GGCATTGTGA GCTCTCGCAA TGAGCTGCGC
    TGCCCAGAGT GCAGGACTCT GGTGGAGTGT GGGGTGGATG AACTTCCGAG CAACATCCTA
    CTAGTGAGAC TGTTGGATGG AATAAAGCAG AGACCAAGAA AAGCAGGGGT TGGTGGCAGT
    GCTGGGAATA GCACAAATGT GCTAAGAGCA CAAGGCAGTC TAACTACTAA TTGTGGCTTA
    AATGATGCAC AGAATATTCA TGGTGGGCAA CAGAGGATAC AGGCACGAAG TCCTCCAGTA
    AGGGGTGTTC CTCAGTTACC ATGTGCCAAA GCTTTGTACA ACTACGAGGG GAAAGAGCCT
    GGAGATCTGA AATTTAACAA GGGAGACATC ATCGTGCTTC GCCGTCAGGT TGATGAAAAT
    TGGTACCACG GGGAGATTAA TGGCATCCAT GGCTTCTTCC CAACAAACTT TGTGCAGATC
    ATAAAGCCTT TACCTCAGCC TCCTCCTCAA TGCAAAGCAC TTTATGACTT TGAAGTGAAA
    GACAAGGAAG CAGACAAGGA TTGCCTACCC TTTTTAAAGG ATGACATTCT CACCGTGATC
    CGACGAGTAG ATGAAAACTG GGCAGAAGGA ATGCTGGGAG ACAAGATAGG AATATTCCCT
    ATTTCCTATG TAGAGTTCAA TTCAGCAGCC AAGCAGCTGA TAGAATTGGA CAAGCCATCA
    GGAGTAGACA CTGGGGAAGG CTCTTCTGGC ACAACGCATA GTAGCAATTC TCAAAAGCAA
    GCAGATGCAA AGAAGAACAC CAAAAAGCGT CACTCCTTTA CCTCCCTCAC CATGTCTAAC
    AAGTCATCGC AGTCCGTGCA GAATCGTCAT TCAATGGAGA TTAGCCCCCC TGTACTCATC
    AGTTCTAGCA ATCCCACCGC TGCTGCCAGG ATCAGTGAAC TCACAGGCCT CTCTTGTAGT
    GCCCCTTCTC AGGTTCATAT CAGTACAACA GGGCTCATTG TGACCCCACC CCCTAGCAGC
    CCTGTTGTAA GTGGCCCTGC ATTTACATTT CCACCGGAAG TCACCTACCA AGCTGCTCTG
    GGAGATTTAA ATCCTCCTCT TCTTCCTCCT CCACCATTGG CCACTCCGGT TATCACGTCG
    ACATCTTCAG GAGCTGCTGC CGCTGTCCAG AGGTCTATCA GCGGTCCAGC TGAGCAAGTC
    ACACACTTGA GGACATCTAC AAGACCCAGT GTGTTTGTTG CTATCTATCC CTATATCCCA
    CGCAAGGAGG ATGAGTTGGA ACTGAGGAAA GGTGAAATGT TCTTGGTTTT TGAGCGCTGT
    CAGGATGGCT GGTTTAAAGG AACTTCCATG CACACCAGCA AAATTGGTGT GTTCCCTGGA
    AACTATGTTG CACCAGTCAC TAGGGCATTA ACAACTGCAA CACCAGCAAA GGTTGCCATG
    GCCACAGCTA GTAGCAGTAA TGTGGTGAAC TTAGTAACGC CAACACCTCC AGGGGCTCCA
    TGCCAAAAAC TGCAAGGGAA TGGAGCGGAG TTTGCCAAGA CTGTCAGTAC AAATGGGGTC
    CCACCGGCTG GCATTCCTGG TTCTCATATC CAGAGTAGCC CACAGGCCAA GGTTCTGCTG
    CACATGAGTG GCCAAATGAC CGTTAACCAA GCCCGCAATG CGGTCCGAAC AGCTGCTGCA
    CACAGCCAGG ATCGTCCAAC AGCAGCTGTG ACTCCCATCC AGGCTCAGAT CCCATCTGCA
    TCTGTTCTCC CCCAGCAGGC TGCCACTTCA CAGCAAATGC CACCTCCGCT TTCAGGTCCA
    GCTGCTTACA TCAATGCTGC AATGAACATT AGCCGACCAA GTGTGCCGGT GGCTTCGGCA
    GCCTCCTCCA GTGTTTCCTC CGCTGCATTT GAAACAGAAT GTAATTGGAA ATCTGGCTCT
    GGACTTGCTG CGTGTTCTTT TCCAGAAAAT GTCTCTGCAC CTTTAAATTC TGCTGCTAAT
    AAGCAAGATA AAGATAGTAA GAAAGAAAAG AAGGGCTTGC TCAAGTTACT GTCTGGGGCA
    TCTACAAAAA GAAAGCCTCG CTCATCTCCG CCGCACTCTC CAACCCAAGA ACTGGAACAG
    ACAAATTCAG AAGCAGCGCT GGAGGGAGCT GTAGGGCCAG ACATTCTGCC TGTCAATGGC
    AATGGCCGAG TTGCTTCCTG CACTGTGGAT TGTGATTTAG TATCTGCCTC AGCTCTGGTA
    CAGGACAACC GCAAGCCAGC TTCCTTGGAT ACCAATGTTC CCATTGCACC TCCACCAAGA
    CAGCCTTGCT CCTCACTGGG CACTGTTCTG AATGACTCCC GGCCTTGTGA AAGATATAGA
    GTTGTAGTGT CCTACCCACC CCAGAGCGAG GCAGAACTAG AGCTGAAGGA AGGGGACATC
    GTATTTGTGC ACAAGAAACG GGAAGATGGT TGGTTTAAAG GCACACTCCA ACGGAACGGC
    AAAACCGGGC TATTTCCCGG CAGCTTTGTG GAGAATATTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa27510
    Clone ID Related Accession (Same CDS sequence) XM_012961871.2 , XM_012961878.3 , XM_012961878.2 , XM_012961871.3
    Accession Version XM_012961878.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase SH3RF1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961878.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTTCTTT CCATGCAAAA CTGTGTCGAT AAAACCATGG ATGAGTCAGC ATTACTGGAC 
    CTCTTAGAAT GTCCAGTGTG TCTTGAGAGA CTTGATGCCT CTGCTAAAGT CTTGCCATGC
    CAACACACAT TCTGCAAGCG CTGCCTTCTG GGCATTGTGA GCTCTCGCAA TGAGCTGCGC
    TGCCCAGAGT GCAGGACTCT GGTGGAGTGT GGGGTGGATG AACTTCCGAG CAACATCCTA
    CTAGTGAGAC TGTTGGATGG AATAAAGCAG AGACCAAGAA AAGCAGGGGT TGGTGGCAGT
    GCTGGGAATA GCACAAATGT GCTAAGAGCA CAAGGCAGTC TAACTACTAA TTGTGGCTTA
    AATGATGCAC AGAATATTCA TGGTGGGCAA CAGAGGATAC AGGCACGAAG TCCTCCAGTA
    AGGGGTGTTC CTCAGTTACC ATGTGCCAAA GCTTTGTACA ACTACGAGGG GAAAGAGCCT
    GGAGATCTGA AATTTAACAA GGGAGACATC ATCGTGCTTC GCCGTCAGGT TGATGAAAAT
    TGGTACCACG GGGAGATTAA TGGCATCCAT GGCTTCTTCC CAACAAACTT TGTGCAGATC
    ATAAAGCCTT TACCTCAGCC TCCTCCTCAA TGCAAAGCAC TTTATGACTT TGAAGTGAAA
    GACAAGGAAG CAGACAAGGA TTGCCTACCC TTTTTAAAGG ATGACATTCT CACCGTGATC
    CGACGAGTAG ATGAAAACTG GGCAGAAGGA ATGCTGGGAG ACAAGATAGG AATATTCCCT
    ATTTCCTATG TAGAGTTCAA TTCAGCAGCC AAGCAGCTGA TAGAATTGGA CAAGCCATCA
    GGAGTAGACA CTGGGGAAGG CTCTTCTGGC ACAACGCATA GTAGCAATTC TCAAAAGCAA
    GCAGATGCAA AGAAGAACAC CAAAAAGCGT CACTCCTTTA CCTCCCTCAC CATGTCTAAC
    AAGTCATCGC AGTCCGTGCA GAATCGTCAT TCAATGGAGA TTAGCCCCCC TGTACTCATC
    AGTTCTAGCA ATCCCACCGC TGCTGCCAGG ATCAGTGAAC TCACAGGCCT CTCTTGTAGT
    GCCCCTTCTC AGGATTTAAA TCCTCCTCTT CTTCCTCCTC CACCATTGGC CACTCCGGTT
    ATCACGTCGA CATCTTCAGG AGCTGCTGCC GCTGTCCAGA GGTCTATCAG CGGTCCAGCT
    GAGCAAGTCA CACACTTGAG GACATCTACA AGACCCAGTG TGTTTGTTGC TATCTATCCC
    TATATCCCAC GCAAGGAGGA TGAGTTGGAA CTGAGGAAAG GTGAAATGTT CTTGGTTTTT
    GAGCGCTGTC AGGATGGCTG GTTTAAAGGA ACTTCCATGC ACACCAGCAA AATTGGTGTG
    TTCCCTGGAA ACTATGTTGC ACCAGTCACT AGGGCATTAA CAACTGCAAC ACCAGCAAAG
    GTTGCCATGG CCACAGCTAG TAGCAGTAAT GTGGTGAACT TAGTAACGCC AACACCTCCA
    GGGGCTCCAT GCCAAAAACT GCAAGGGAAT GGAGCGGAGT TTGCCAAGAC TGTCAGTACA
    AATGGGGTCC CACCGGCTGG CATTCCTGGT TCTCATATCC AGAGTAGCCC ACAGGCCAAG
    GTTCTGCTGC ACATGAGTGG CCAAATGACC GTTAACCAAG CCCGCAATGC GGTCCGAACA
    GCTGCTGCAC ACAGCCAGGA TCGTCCAACA GCAGCTGTGA CTCCCATCCA GGCTCAGATC
    CCATCTGCAT CTGTTCTCCC CCAGCAGGCT GCCACTTCAC AGCAAATGCC ACCTCCGCTT
    TCAGGTCCAG CTGCTTACAT CAATGCTGCA ATGAACATTA GCCGACCAAG TGTGCCGGTG
    GCTTCGGCAG CCTCCTCCAG TGTTTCCTCC GCTGCATTTG AAACAGAATG TAATTGGAAA
    TCTGGCTCTG GACTTGCTGC GTGTTCTTTT CCAGAAAATG TCTCTGCACC TTTAAATTCT
    GCTGCTAATA AGCAAGATAA AGATAGTAAG AAAGAAAAGA AGGGCTTGCT CAAGTTACTG
    TCTGGGGCAT CTACAAAAAG AAAGCCTCGC TCATCTCCGC CGCACTCTCC AACCCAAGAA
    CTGGAACAGA CAAATTCAGA AGCAGCGCTG GAGGGAGCTG TAGGGCCAGA CATTCTGCCT
    GTCAATGGCA ATGGCCGAGT TGCTTCCTGC ACTGTGGATT GTGATTTAGT ATCTGCCTCA
    GCTCTGGTAC AGGACAACCG CAAGCCAGCT TCCTTGGATA CCAATGTTCC CATTGCACCT
    CCACCAAGAC AGCCTTGCTC CTCACTGGGC ACTGTTCTGA ATGACTCCCG GCCTTGTGAA
    AGATATAGAG TTGTAGTGTC CTACCCACCC CAGAGCGAGG CAGAACTAGA GCTGAAGGAA
    GGGGACATCG TATTTGTGCA CAAGAAACGG GAAGATGGTT GGTTTAAAGG CACACTCCAA
    CGGAACGGCA AAACCGGGCT ATTTCCCGGC AGCTTTGTGG AGAATATTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817332.1
    CDS392..2902
    Translation

    Target ORF information:

    RefSeq Version XM_012961878.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis SH3 domain containing ring finger 1 (sh3rf1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961878.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTTCTTT CCATGCAAAA CTGTGTCGAT AAAACCATGG ATGAGTCAGC ATTACTGGAC 
    CTCTTAGAAT GTCCAGTGTG TCTTGAGAGA CTTGATGCCT CTGCTAAAGT CTTGCCATGC
    CAACACACAT TCTGCAAGCG CTGCCTTCTG GGCATTGTGA GCTCTCGCAA TGAGCTGCGC
    TGCCCAGAGT GCAGGACTCT GGTGGAGTGT GGGGTGGATG AACTTCCGAG CAACATCCTA
    CTAGTGAGAC TGTTGGATGG AATAAAGCAG AGACCAAGAA AAGCAGGGGT TGGTGGCAGT
    GCTGGGAATA GCACAAATGT GCTAAGAGCA CAAGGCAGTC TAACTACTAA TTGTGGCTTA
    AATGATGCAC AGAATATTCA TGGTGGGCAA CAGAGGATAC AGGCACGAAG TCCTCCAGTA
    AGGGGTGTTC CTCAGTTACC ATGTGCCAAA GCTTTGTACA ACTACGAGGG GAAAGAGCCT
    GGAGATCTGA AATTTAACAA GGGAGACATC ATCGTGCTTC GCCGTCAGGT TGATGAAAAT
    TGGTACCACG GGGAGATTAA TGGCATCCAT GGCTTCTTCC CAACAAACTT TGTGCAGATC
    ATAAAGCCTT TACCTCAGCC TCCTCCTCAA TGCAAAGCAC TTTATGACTT TGAAGTGAAA
    GACAAGGAAG CAGACAAGGA TTGCCTACCC TTTTTAAAGG ATGACATTCT CACCGTGATC
    CGACGAGTAG ATGAAAACTG GGCAGAAGGA ATGCTGGGAG ACAAGATAGG AATATTCCCT
    ATTTCCTATG TAGAGTTCAA TTCAGCAGCC AAGCAGCTGA TAGAATTGGA CAAGCCATCA
    GGAGTAGACA CTGGGGAAGG CTCTTCTGGC ACAACGCATA GTAGCAATTC TCAAAAGCAA
    GCAGATGCAA AGAAGAACAC CAAAAAGCGT CACTCCTTTA CCTCCCTCAC CATGTCTAAC
    AAGTCATCGC AGTCCGTGCA GAATCGTCAT TCAATGGAGA TTAGCCCCCC TGTACTCATC
    AGTTCTAGCA ATCCCACCGC TGCTGCCAGG ATCAGTGAAC TCACAGGCCT CTCTTGTAGT
    GCCCCTTCTC AGGATTTAAA TCCTCCTCTT CTTCCTCCTC CACCATTGGC CACTCCGGTT
    ATCACGTCGA CATCTTCAGG AGCTGCTGCC GCTGTCCAGA GGTCTATCAG CGGTCCAGCT
    GAGCAAGTCA CACACTTGAG GACATCTACA AGACCCAGTG TGTTTGTTGC TATCTATCCC
    TATATCCCAC GCAAGGAGGA TGAGTTGGAA CTGAGGAAAG GTGAAATGTT CTTGGTTTTT
    GAGCGCTGTC AGGATGGCTG GTTTAAAGGA ACTTCCATGC ACACCAGCAA AATTGGTGTG
    TTCCCTGGAA ACTATGTTGC ACCAGTCACT AGGGCATTAA CAACTGCAAC ACCAGCAAAG
    GTTGCCATGG CCACAGCTAG TAGCAGTAAT GTGGTGAACT TAGTAACGCC AACACCTCCA
    GGGGCTCCAT GCCAAAAACT GCAAGGGAAT GGAGCGGAGT TTGCCAAGAC TGTCAGTACA
    AATGGGGTCC CACCGGCTGG CATTCCTGGT TCTCATATCC AGAGTAGCCC ACAGGCCAAG
    GTTCTGCTGC ACATGAGTGG CCAAATGACC GTTAACCAAG CCCGCAATGC GGTCCGAACA
    GCTGCTGCAC ACAGCCAGGA TCGTCCAACA GCAGCTGTGA CTCCCATCCA GGCTCAGATC
    CCATCTGCAT CTGTTCTCCC CCAGCAGGCT GCCACTTCAC AGCAAATGCC ACCTCCGCTT
    TCAGGTCCAG CTGCTTACAT CAATGCTGCA ATGAACATTA GCCGACCAAG TGTGCCGGTG
    GCTTCGGCAG CCTCCTCCAG TGTTTCCTCC GCTGCATTTG AAACAGAATG TAATTGGAAA
    TCTGGCTCTG GACTTGCTGC GTGTTCTTTT CCAGAAAATG TCTCTGCACC TTTAAATTCT
    GCTGCTAATA AGCAAGATAA AGATAGTAAG AAAGAAAAGA AGGGCTTGCT CAAGTTACTG
    TCTGGGGCAT CTACAAAAAG AAAGCCTCGC TCATCTCCGC CGCACTCTCC AACCCAAGAA
    CTGGAACAGA CAAATTCAGA AGCAGCGCTG GAGGGAGCTG TAGGGCCAGA CATTCTGCCT
    GTCAATGGCA ATGGCCGAGT TGCTTCCTGC ACTGTGGATT GTGATTTAGT ATCTGCCTCA
    GCTCTGGTAC AGGACAACCG CAAGCCAGCT TCCTTGGATA CCAATGTTCC CATTGCACCT
    CCACCAAGAC AGCCTTGCTC CTCACTGGGC ACTGTTCTGA ATGACTCCCG GCCTTGTGAA
    AGATATAGAG TTGTAGTGTC CTACCCACCC CAGAGCGAGG CAGAACTAGA GCTGAAGGAA
    GGGGACATCG TATTTGTGCA CAAGAAACGG GAAGATGGTT GGTTTAAAGG CACACTCCAA
    CGGAACGGCA AAACCGGGCT ATTTCCCGGC AGCTTTGTGG AGAATATTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa27510
    Clone ID Related Accession (Same CDS sequence) XM_012961871.2 , XM_012961878.3 , XM_012961878.2 , XM_012961871.3
    Accession Version XM_012961871.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase SH3RF1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961871.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTTCTTT CCATGCAAAA CTGTGTCGAT AAAACCATGG ATGAGTCAGC ATTACTGGAC 
    CTCTTAGAAT GTCCAGTGTG TCTTGAGAGA CTTGATGCCT CTGCTAAAGT CTTGCCATGC
    CAACACACAT TCTGCAAGCG CTGCCTTCTG GGCATTGTGA GCTCTCGCAA TGAGCTGCGC
    TGCCCAGAGT GCAGGACTCT GGTGGAGTGT GGGGTGGATG AACTTCCGAG CAACATCCTA
    CTAGTGAGAC TGTTGGATGG AATAAAGCAG AGACCAAGAA AAGCAGGGGT TGGTGGCAGT
    GCTGGGAATA GCACAAATGT GCTAAGAGCA CAAGGCAGTC TAACTACTAA TTGTGGCTTA
    AATGATGCAC AGAATATTCA TGGTGGGCAA CAGAGGATAC AGGCACGAAG TCCTCCAGTA
    AGGGGTGTTC CTCAGTTACC ATGTGCCAAA GCTTTGTACA ACTACGAGGG GAAAGAGCCT
    GGAGATCTGA AATTTAACAA GGGAGACATC ATCGTGCTTC GCCGTCAGGT TGATGAAAAT
    TGGTACCACG GGGAGATTAA TGGCATCCAT GGCTTCTTCC CAACAAACTT TGTGCAGATC
    ATAAAGCCTT TACCTCAGCC TCCTCCTCAA TGCAAAGCAC TTTATGACTT TGAAGTGAAA
    GACAAGGAAG CAGACAAGGA TTGCCTACCC TTTTTAAAGG ATGACATTCT CACCGTGATC
    CGACGAGTAG ATGAAAACTG GGCAGAAGGA ATGCTGGGAG ACAAGATAGG AATATTCCCT
    ATTTCCTATG TAGAGTTCAA TTCAGCAGCC AAGCAGCTGA TAGAATTGGA CAAGCCATCA
    GGAGTAGACA CTGGGGAAGG CTCTTCTGGC ACAACGCATA GTAGCAATTC TCAAAAGCAA
    GCAGATGCAA AGAAGAACAC CAAAAAGCGT CACTCCTTTA CCTCCCTCAC CATGTCTAAC
    AAGTCATCGC AGTCCGTGCA GAATCGTCAT TCAATGGAGA TTAGCCCCCC TGTACTCATC
    AGTTCTAGCA ATCCCACCGC TGCTGCCAGG ATCAGTGAAC TCACAGGCCT CTCTTGTAGT
    GCCCCTTCTC AGGATTTAAA TCCTCCTCTT CTTCCTCCTC CACCATTGGC CACTCCGGTT
    ATCACGTCGA CATCTTCAGG AGCTGCTGCC GCTGTCCAGA GGTCTATCAG CGGTCCAGCT
    GAGCAAGTCA CACACTTGAG GACATCTACA AGACCCAGTG TGTTTGTTGC TATCTATCCC
    TATATCCCAC GCAAGGAGGA TGAGTTGGAA CTGAGGAAAG GTGAAATGTT CTTGGTTTTT
    GAGCGCTGTC AGGATGGCTG GTTTAAAGGA ACTTCCATGC ACACCAGCAA AATTGGTGTG
    TTCCCTGGAA ACTATGTTGC ACCAGTCACT AGGGCATTAA CAACTGCAAC ACCAGCAAAG
    GTTGCCATGG CCACAGCTAG TAGCAGTAAT GTGGTGAACT TAGTAACGCC AACACCTCCA
    GGGGCTCCAT GCCAAAAACT GCAAGGGAAT GGAGCGGAGT TTGCCAAGAC TGTCAGTACA
    AATGGGGTCC CACCGGCTGG CATTCCTGGT TCTCATATCC AGAGTAGCCC ACAGGCCAAG
    GTTCTGCTGC ACATGAGTGG CCAAATGACC GTTAACCAAG CCCGCAATGC GGTCCGAACA
    GCTGCTGCAC ACAGCCAGGA TCGTCCAACA GCAGCTGTGA CTCCCATCCA GGCTCAGATC
    CCATCTGCAT CTGTTCTCCC CCAGCAGGCT GCCACTTCAC AGCAAATGCC ACCTCCGCTT
    TCAGGTCCAG CTGCTTACAT CAATGCTGCA ATGAACATTA GCCGACCAAG TGTGCCGGTG
    GCTTCGGCAG CCTCCTCCAG TGTTTCCTCC GCTGCATTTG AAACAGAATG TAATTGGAAA
    TCTGGCTCTG GACTTGCTGC GTGTTCTTTT CCAGAAAATG TCTCTGCACC TTTAAATTCT
    GCTGCTAATA AGCAAGATAA AGATAGTAAG AAAGAAAAGA AGGGCTTGCT CAAGTTACTG
    TCTGGGGCAT CTACAAAAAG AAAGCCTCGC TCATCTCCGC CGCACTCTCC AACCCAAGAA
    CTGGAACAGA CAAATTCAGA AGCAGCGCTG GAGGGAGCTG TAGGGCCAGA CATTCTGCCT
    GTCAATGGCA ATGGCCGAGT TGCTTCCTGC ACTGTGGATT GTGATTTAGT ATCTGCCTCA
    GCTCTGGTAC AGGACAACCG CAAGCCAGCT TCCTTGGATA CCAATGTTCC CATTGCACCT
    CCACCAAGAC AGCCTTGCTC CTCACTGGGC ACTGTTCTGA ATGACTCCCG GCCTTGTGAA
    AGATATAGAG TTGTAGTGTC CTACCCACCC CAGAGCGAGG CAGAACTAGA GCTGAAGGAA
    GGGGACATCG TATTTGTGCA CAAGAAACGG GAAGATGGTT GGTTTAAAGG CACACTCCAA
    CGGAACGGCA AAACCGGGCT ATTTCCCGGC AGCTTTGTGG AGAATATTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817325.1
    CDS422..2932
    Translation

    Target ORF information:

    RefSeq Version XM_012961871.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis SH3 domain containing ring finger 1 (sh3rf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012961871.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTTCTTT CCATGCAAAA CTGTGTCGAT AAAACCATGG ATGAGTCAGC ATTACTGGAC 
    CTCTTAGAAT GTCCAGTGTG TCTTGAGAGA CTTGATGCCT CTGCTAAAGT CTTGCCATGC
    CAACACACAT TCTGCAAGCG CTGCCTTCTG GGCATTGTGA GCTCTCGCAA TGAGCTGCGC
    TGCCCAGAGT GCAGGACTCT GGTGGAGTGT GGGGTGGATG AACTTCCGAG CAACATCCTA
    CTAGTGAGAC TGTTGGATGG AATAAAGCAG AGACCAAGAA AAGCAGGGGT TGGTGGCAGT
    GCTGGGAATA GCACAAATGT GCTAAGAGCA CAAGGCAGTC TAACTACTAA TTGTGGCTTA
    AATGATGCAC AGAATATTCA TGGTGGGCAA CAGAGGATAC AGGCACGAAG TCCTCCAGTA
    AGGGGTGTTC CTCAGTTACC ATGTGCCAAA GCTTTGTACA ACTACGAGGG GAAAGAGCCT
    GGAGATCTGA AATTTAACAA GGGAGACATC ATCGTGCTTC GCCGTCAGGT TGATGAAAAT
    TGGTACCACG GGGAGATTAA TGGCATCCAT GGCTTCTTCC CAACAAACTT TGTGCAGATC
    ATAAAGCCTT TACCTCAGCC TCCTCCTCAA TGCAAAGCAC TTTATGACTT TGAAGTGAAA
    GACAAGGAAG CAGACAAGGA TTGCCTACCC TTTTTAAAGG ATGACATTCT CACCGTGATC
    CGACGAGTAG ATGAAAACTG GGCAGAAGGA ATGCTGGGAG ACAAGATAGG AATATTCCCT
    ATTTCCTATG TAGAGTTCAA TTCAGCAGCC AAGCAGCTGA TAGAATTGGA CAAGCCATCA
    GGAGTAGACA CTGGGGAAGG CTCTTCTGGC ACAACGCATA GTAGCAATTC TCAAAAGCAA
    GCAGATGCAA AGAAGAACAC CAAAAAGCGT CACTCCTTTA CCTCCCTCAC CATGTCTAAC
    AAGTCATCGC AGTCCGTGCA GAATCGTCAT TCAATGGAGA TTAGCCCCCC TGTACTCATC
    AGTTCTAGCA ATCCCACCGC TGCTGCCAGG ATCAGTGAAC TCACAGGCCT CTCTTGTAGT
    GCCCCTTCTC AGGATTTAAA TCCTCCTCTT CTTCCTCCTC CACCATTGGC CACTCCGGTT
    ATCACGTCGA CATCTTCAGG AGCTGCTGCC GCTGTCCAGA GGTCTATCAG CGGTCCAGCT
    GAGCAAGTCA CACACTTGAG GACATCTACA AGACCCAGTG TGTTTGTTGC TATCTATCCC
    TATATCCCAC GCAAGGAGGA TGAGTTGGAA CTGAGGAAAG GTGAAATGTT CTTGGTTTTT
    GAGCGCTGTC AGGATGGCTG GTTTAAAGGA ACTTCCATGC ACACCAGCAA AATTGGTGTG
    TTCCCTGGAA ACTATGTTGC ACCAGTCACT AGGGCATTAA CAACTGCAAC ACCAGCAAAG
    GTTGCCATGG CCACAGCTAG TAGCAGTAAT GTGGTGAACT TAGTAACGCC AACACCTCCA
    GGGGCTCCAT GCCAAAAACT GCAAGGGAAT GGAGCGGAGT TTGCCAAGAC TGTCAGTACA
    AATGGGGTCC CACCGGCTGG CATTCCTGGT TCTCATATCC AGAGTAGCCC ACAGGCCAAG
    GTTCTGCTGC ACATGAGTGG CCAAATGACC GTTAACCAAG CCCGCAATGC GGTCCGAACA
    GCTGCTGCAC ACAGCCAGGA TCGTCCAACA GCAGCTGTGA CTCCCATCCA GGCTCAGATC
    CCATCTGCAT CTGTTCTCCC CCAGCAGGCT GCCACTTCAC AGCAAATGCC ACCTCCGCTT
    TCAGGTCCAG CTGCTTACAT CAATGCTGCA ATGAACATTA GCCGACCAAG TGTGCCGGTG
    GCTTCGGCAG CCTCCTCCAG TGTTTCCTCC GCTGCATTTG AAACAGAATG TAATTGGAAA
    TCTGGCTCTG GACTTGCTGC GTGTTCTTTT CCAGAAAATG TCTCTGCACC TTTAAATTCT
    GCTGCTAATA AGCAAGATAA AGATAGTAAG AAAGAAAAGA AGGGCTTGCT CAAGTTACTG
    TCTGGGGCAT CTACAAAAAG AAAGCCTCGC TCATCTCCGC CGCACTCTCC AACCCAAGAA
    CTGGAACAGA CAAATTCAGA AGCAGCGCTG GAGGGAGCTG TAGGGCCAGA CATTCTGCCT
    GTCAATGGCA ATGGCCGAGT TGCTTCCTGC ACTGTGGATT GTGATTTAGT ATCTGCCTCA
    GCTCTGGTAC AGGACAACCG CAAGCCAGCT TCCTTGGATA CCAATGTTCC CATTGCACCT
    CCACCAAGAC AGCCTTGCTC CTCACTGGGC ACTGTTCTGA ATGACTCCCG GCCTTGTGAA
    AGATATAGAG TTGTAGTGTC CTACCCACCC CAGAGCGAGG CAGAACTAGA GCTGAAGGAA
    GGGGACATCG TATTTGTGCA CAAGAAACGG GAAGATGGTT GGTTTAAAGG CACACTCCAA
    CGGAACGGCA AAACCGGGCT ATTTCCCGGC AGCTTTGTGG AGAATATTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa41795
    Clone ID Related Accession (Same CDS sequence) XM_018093029.1
    Accession Version XM_018093029.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2472bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase SH3RF1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTTCTTT CCATGCAAAA CTGTGTCGAT AAAACCATGG ATGAGTCAGC ATTACTGGAC 
    CTCTTAGAAT GTCCAGTGTG TCTTGAGAGA CTTGATGCCT CTGCTAAAGT CTTGCCATGC
    CAACACACAT TCTGCAAGCG CTGCCTTCTG GGCATTGTGA GCTCTCGCAA TGAGCTGCGC
    TGCCCAGAGT GCAGGACTCT GGTGGAGTGT GGGGTGGATG AACTTCCGAG CAACATCCTA
    CTAGTGAGAC TGTTGGATGG AATAAAGCAG AGACCAAGAA AAGCAGGGGT TGGTGGCAGT
    GCTGGGAATA GCACAAATGT GCTAAGAGCA CAAGGCAGTC TAACTACTAA TTGTGGCTTA
    AATGATGCAC AGAATATTCA TGGTGGGCAA CAGAGGATAC AGGCACGAAG TCCTCCAGTA
    AGGGGTGTTC CTCAGTTACC ATGTGCCAAA GCTTTGTACA ACTACGAGGG GAAAGAGCCT
    GGAGATCTGA AATTTAACAA GGGAGACATC ATCGTGCTTC GCCGTCAGGT TGATGAAAAT
    TGGTACCACG GGGAGATTAA TGGCATCCAT GGCTTCTTCC CAACAAACTT TGTGCAGATC
    ATAAAGCCTT TACCTCAGCC TCCTCCTCAA TGCAAAGCAC TTTATGACTT TGAAGTGAAA
    GACAAGGAAG CAGACAAGGA TTGCCTACCC TTTTTAAAGG ATGACATTCT CACCGTGATC
    CGACGAGTAG ATGAAAACTG GGCAGAAGGA ATGCTGGGAG ACAAGATAGG AATATTCCCT
    ATTTCCTATG TAGAGTTCAA TTCAGCAGCC AAGCAGCTGA TAGAATTGGA CAAGCCATCA
    GGAGTAGACA CTGGGGAAGG CTCTTCTGGC ACAACGCATA GTAGCAATTC TCAAAAGCAA
    GCAGATGCAA AGAAGAACAC CAAAAAGCGT CACTCCTTTA CCTCCCTCAC CATGTCTAAC
    AAGTCATCGC AGTCCGTGCA GAATCGTCAT TCAATGGAGA TTAGCCCCCC TGTACTCATC
    AGTTCTAGCA ATCCCACCGC TGCTGCCAGG ATCAGTGAAC TCACAGGCCT CTCTTGTAGT
    GCCCCTTCTC AGGTTCATAT CAGTACAACA GGGCTCATTG TGACCCCACC CCCTAGCAGC
    CCTGTTGTAA GTGGCCCTGC ATTTACATTT CCACCGGAAG TCACCTACCA AGCTGCTCTG
    GGAGATTTAA ATCCTCCTCT TCTTCCTCCT CCACCATTGG CCACTCCGGT TATCACGTCG
    ACATCTTCAG GAGCTGCTGC CGCTGTCCAG AGGTCTATCA GCGGTCCAGC TGAGCAAGTC
    ACACACTTGA GGACATCTAC AAGACCCAGT GTGTTTGTTG CTATCTATCC CTATATCCCA
    CGCAAGGAGG ATGAGTTGGA ACTGAGGAAA GGTGAAATGT TCTTGGTTTT TGAGCGCTGT
    CAGGATGGCT GGTTTAAAGG AACTTCCATG CACACCAGCA AAATTGGTGT GTTCCCTGGA
    AACTATGTTG CACCAGTCAC TAGGGCATTA ACAACTGCAA CACCAGCAAA GGTTGCCATG
    GCCACAGCTA GTAGCAGTAA TGTGGTGAAC TTAGTAACGC CAACACCTCC AGGGGCTCCA
    TGCCAAAAAC TGCAAGGGAA TGGAGCGGAG TTTGCCAAGA CTGTCAGTAC AAATGGGGTC
    CCACCGGCTG GCATTCCTGG TTCTCATATC CAGAGTAGCC CACAGGCCAA GGTTCTGCTG
    CACATGAGTG GCCAAATGAC CGTTAACCAA GCCCGCAATG CGGTCCGAAC AGCTGCTGCA
    CACAGCCAGG ATCGTCCAAC AGCAGCTGTG ACTCCCATCC AGGCTCAGAT CCCATCTGCA
    TCTGTTCTCC CCCAGCAGGC TGCCACTTCA CAGCAAATGC CACCTCCGCT TTCAGGTCCA
    GCTGCTTACA TCAATGCTGC AATGAACATT AGCCGACCAA GTGTGCCGGT GGCTTCGGCA
    GCCTCCTCCA GTGTTTCCTC CGCTGCATTT GAAACAGAAT GTAATTGGAA ATCTGGCTCT
    GGACTTGCTG CGTGTTCTTT TCCAGAAAAT GTCTCTGCAC CTTTAAATTC TGCTGCTAAT
    AAGCAAGATA AAGATAGTAA GAAAGAAAAG AAGGGCTTGC TCAAGTTACT GTCTGGGGCA
    TCTACAAAAA GAAAGCCTCG CTCATCTCCG CCGCACTCTC CAACCCAAGA ACTGGAACAG
    ACAAATTCAG AAGCAGCGCT GGAGGGAGCT GTAGGGCCAG ACATTCTGCC TGTCAATGGC
    AATGGCCGAG TTGCTTCCTG CACTGTGGAT TGTGATTTAG TATCTGCCTC AGCTCTGGTA
    CAGGACAACC GCAAGCCAGC TTCCTTGGAT ACCAATGTTC CCATTGCACC TCCACCAAGA
    CAGCCTTGCT CCTCACTGGG CACTGTTCTG AATGACTCCC GGCCTTGTGA AAGAAAGGCA
    ACCAAAACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948518.1
    CDS619..3090
    Translation

    Target ORF information:

    RefSeq Version XM_018093029.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis SH3 domain containing ring finger 1 (sh3rf1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018093029.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTTCTTT CCATGCAAAA CTGTGTCGAT AAAACCATGG ATGAGTCAGC ATTACTGGAC 
    CTCTTAGAAT GTCCAGTGTG TCTTGAGAGA CTTGATGCCT CTGCTAAAGT CTTGCCATGC
    CAACACACAT TCTGCAAGCG CTGCCTTCTG GGCATTGTGA GCTCTCGCAA TGAGCTGCGC
    TGCCCAGAGT GCAGGACTCT GGTGGAGTGT GGGGTGGATG AACTTCCGAG CAACATCCTA
    CTAGTGAGAC TGTTGGATGG AATAAAGCAG AGACCAAGAA AAGCAGGGGT TGGTGGCAGT
    GCTGGGAATA GCACAAATGT GCTAAGAGCA CAAGGCAGTC TAACTACTAA TTGTGGCTTA
    AATGATGCAC AGAATATTCA TGGTGGGCAA CAGAGGATAC AGGCACGAAG TCCTCCAGTA
    AGGGGTGTTC CTCAGTTACC ATGTGCCAAA GCTTTGTACA ACTACGAGGG GAAAGAGCCT
    GGAGATCTGA AATTTAACAA GGGAGACATC ATCGTGCTTC GCCGTCAGGT TGATGAAAAT
    TGGTACCACG GGGAGATTAA TGGCATCCAT GGCTTCTTCC CAACAAACTT TGTGCAGATC
    ATAAAGCCTT TACCTCAGCC TCCTCCTCAA TGCAAAGCAC TTTATGACTT TGAAGTGAAA
    GACAAGGAAG CAGACAAGGA TTGCCTACCC TTTTTAAAGG ATGACATTCT CACCGTGATC
    CGACGAGTAG ATGAAAACTG GGCAGAAGGA ATGCTGGGAG ACAAGATAGG AATATTCCCT
    ATTTCCTATG TAGAGTTCAA TTCAGCAGCC AAGCAGCTGA TAGAATTGGA CAAGCCATCA
    GGAGTAGACA CTGGGGAAGG CTCTTCTGGC ACAACGCATA GTAGCAATTC TCAAAAGCAA
    GCAGATGCAA AGAAGAACAC CAAAAAGCGT CACTCCTTTA CCTCCCTCAC CATGTCTAAC
    AAGTCATCGC AGTCCGTGCA GAATCGTCAT TCAATGGAGA TTAGCCCCCC TGTACTCATC
    AGTTCTAGCA ATCCCACCGC TGCTGCCAGG ATCAGTGAAC TCACAGGCCT CTCTTGTAGT
    GCCCCTTCTC AGGTTCATAT CAGTACAACA GGGCTCATTG TGACCCCACC CCCTAGCAGC
    CCTGTTGTAA GTGGCCCTGC ATTTACATTT CCACCGGAAG TCACCTACCA AGCTGCTCTG
    GGAGATTTAA ATCCTCCTCT TCTTCCTCCT CCACCATTGG CCACTCCGGT TATCACGTCG
    ACATCTTCAG GAGCTGCTGC CGCTGTCCAG AGGTCTATCA GCGGTCCAGC TGAGCAAGTC
    ACACACTTGA GGACATCTAC AAGACCCAGT GTGTTTGTTG CTATCTATCC CTATATCCCA
    CGCAAGGAGG ATGAGTTGGA ACTGAGGAAA GGTGAAATGT TCTTGGTTTT TGAGCGCTGT
    CAGGATGGCT GGTTTAAAGG AACTTCCATG CACACCAGCA AAATTGGTGT GTTCCCTGGA
    AACTATGTTG CACCAGTCAC TAGGGCATTA ACAACTGCAA CACCAGCAAA GGTTGCCATG
    GCCACAGCTA GTAGCAGTAA TGTGGTGAAC TTAGTAACGC CAACACCTCC AGGGGCTCCA
    TGCCAAAAAC TGCAAGGGAA TGGAGCGGAG TTTGCCAAGA CTGTCAGTAC AAATGGGGTC
    CCACCGGCTG GCATTCCTGG TTCTCATATC CAGAGTAGCC CACAGGCCAA GGTTCTGCTG
    CACATGAGTG GCCAAATGAC CGTTAACCAA GCCCGCAATG CGGTCCGAAC AGCTGCTGCA
    CACAGCCAGG ATCGTCCAAC AGCAGCTGTG ACTCCCATCC AGGCTCAGAT CCCATCTGCA
    TCTGTTCTCC CCCAGCAGGC TGCCACTTCA CAGCAAATGC CACCTCCGCT TTCAGGTCCA
    GCTGCTTACA TCAATGCTGC AATGAACATT AGCCGACCAA GTGTGCCGGT GGCTTCGGCA
    GCCTCCTCCA GTGTTTCCTC CGCTGCATTT GAAACAGAAT GTAATTGGAA ATCTGGCTCT
    GGACTTGCTG CGTGTTCTTT TCCAGAAAAT GTCTCTGCAC CTTTAAATTC TGCTGCTAAT
    AAGCAAGATA AAGATAGTAA GAAAGAAAAG AAGGGCTTGC TCAAGTTACT GTCTGGGGCA
    TCTACAAAAA GAAAGCCTCG CTCATCTCCG CCGCACTCTC CAACCCAAGA ACTGGAACAG
    ACAAATTCAG AAGCAGCGCT GGAGGGAGCT GTAGGGCCAG ACATTCTGCC TGTCAATGGC
    AATGGCCGAG TTGCTTCCTG CACTGTGGAT TGTGATTTAG TATCTGCCTC AGCTCTGGTA
    CAGGACAACC GCAAGCCAGC TTCCTTGGAT ACCAATGTTC CCATTGCACC TCCACCAAGA
    CAGCCTTGCT CCTCACTGGG CACTGTTCTG AATGACTCCC GGCCTTGTGA AAGAAAGGCA
    ACCAAAACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa41796
    Clone ID Related Accession (Same CDS sequence) XM_018093030.1
    Accession Version XM_018093030.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase SH3RF1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTTCTTT CCATGCAAAA CTGTGTCGAT AAAACCATGG ATGAGTCAGC ATTACTGGAC 
    CTCTTAGAAT GTCCAGTGTG TCTTGAGAGA CTTGATGCCT CTGCTAAAGT CTTGCCATGC
    CAACACACAT TCTGCAAGCG CTGCCTTCTG GGCATTGTGA GCTCTCGCAA TGAGCTGCGC
    TGCCCAGAGT GCAGGACTCT GGTGGAGTGT GGGGTGGATG AACTTCCGAG CAACATCCTA
    CTAGTGAGAC TGTTGGATGG AATAAAGCAG AGACCAAGAA AAGCAGGGGT TGGTGGCAGT
    GCTGGGAATA GCACAAATGT GCTAAGAGCA CAAGGCAGTC TAACTACTAA TTGTGGCTTA
    AATGATGCAC AGAATATTCA TGGTGGGCAA CAGAGGATAC AGGCACGAAG TCCTCCAGTA
    AGGGGTGTTC CTCAGTTACC ATGTGCCAAA GCTTTGTACA ACTACGAGGG GAAAGAGCCT
    GGAGATCTGA AATTTAACAA GGGAGACATC ATCGTGCTTC GCCGTCAGGT TGATGAAAAT
    TGGTACCACG GGGAGATTAA TGGCATCCAT GGCTTCTTCC CAACAAACTT TGTGCAGATC
    ATAAAGCCTT TACCTCAGCC TCCTCCTCAA TGCAAAGCAC TTTATGACTT TGAAGTGAAA
    GACAAGGAAG CAGACAAGGA TTGCCTACCC TTTTTAAAGG ATGACATTCT CACCGTGATC
    CGACGAGTAG ATGAAAACTG GGCAGAAGGA ATGCTGGGAG ACAAGATAGG AATATTCCCT
    ATTTCCTATG TAGAGTTCAA TTCAGCAGCC AAGCAGCTGA TAGAATTGGA CAAGCCATCA
    GGAGTAGACA CTGGGGAAGG CTCTTCTGGC ACAACGCATA GTAGCAATTC TCAAAAGCAA
    GCAGATGCAA AGAAGAACAC CAAAAAGCGT CACTCCTTTA CCTCCCTCAC CATGTCTAAC
    AAGTCATCGC AGTCCGTGCA GAATCGTCAT TCAATGGAGA TTAGCCCCCC TGTACTCATC
    AGTTCTAGCA ATCCCACCGC TGCTGCCAGG ATCAGTGAAC TCACAGGCCT CTCTTGTAGT
    GCCCCTTCTC AGGTTCATAT CAGTACAACA GGGCTCATTG TGACCCCACC CCCTAGCAGC
    CCTGTTGTAA GTGGCCCTGC ATTTACATTT CCACCGGAAG TCACCTACCA AGCTGCTCTG
    GGAGATTTAA ATCCTCCTCT TCTTCCTCCT CCACCATTGG CCACTCCGGT TATCACGTCG
    ACATCTTCAG GAGCTGCTGC CGCTGTCCAG AGGTCTATCA GCGGTCCAGC TGAGCAAGTC
    ACACACTTGA GGACATCTAC AAGACCCAGT GTGGCATTAA CAACTGCAAC ACCAGCAAAG
    GTTGCCATGG CCACAGCTAG TAGCAGTAAT GTGGTGAACT TAGTAACGCC AACACCTCCA
    GGGGCTCCAT GCCAAAAACT GCAAGGGAAT GGAGCGGAGT TTGCCAAGAC TGTCAGTACA
    AATGGGGTCC CACCGGCTGG CATTCCTGGT TCTCATATCC AGAGTAGCCC ACAGGCCAAG
    GTTCTGCTGC ACATGAGTGG CCAAATGACC GTTAACCAAG CCCGCAATGC GGTCCGAACA
    GCTGCTGCAC ACAGCCAGGA TCGTCCAACA GCAGCTGTGA CTCCCATCCA GGCTCAGATC
    CCATCTGCAT CTGTTCTCCC CCAGCAGGCT GCCACTTCAC AGCAAATGCC ACCTCCGCTT
    TCAGGTCCAG CTGCTTACAT CAATGCTGCA ATGAACATTA GCCGACCAAG TGTGCCGGTG
    GCTTCGGCAG CCTCCTCCAG TGTTTCCTCC GCTGCATTTG AAACAGAATG TAATTGGAAA
    TCTGGCTCTG GACTTGCTGC GTGTTCTTTT CCAGAAAATG TCTCTGCACC TTTAAATTCT
    GCTGCTAATA AGCAAGATAA AGATAGTAAG AAAGAAAAGA AGGGCTTGCT CAAGTTACTG
    TCTGGGGCAT CTACAAAAAG AAAGCCTCGC TCATCTCCGC CGCACTCTCC AACCCAAGAA
    CTGGAACAGA CAAATTCAGA AGCAGCGCTG GAGGGAGCTG TAGGGCCAGA CATTCTGCCT
    GTCAATGGCA ATGGCCGAGT TGCTTCCTGC ACTGTGGATT GTGATTTAGT ATCTGCCTCA
    GCTCTGGTAC AGGACAACCG CAAGCCAGCT TCCTTGGATA CCAATGTTCC CATTGCACCT
    CCACCAAGAC AGCCTTGCTC CTCACTGGGC ACTGTTCTGA ATGACTCCCG GCCTTGTGAA
    AGATATAGAG TTGTAGTGTC CTACCCACCC CAGAGCGAGG CAGAACTAGA GCTGAAGGAA
    GGGGACATCG TATTTGTGCA CAAGAAACGG GAAGATGGTT GGTTTAAAGG CACACTCCAA
    CGGAACGGCA AAACCGGGCT ATTTCCCGGC AGCTTTGTGG AGAATATTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948519.1
    CDS615..3065
    Translation

    Target ORF information:

    RefSeq Version XM_018093030.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis SH3 domain containing ring finger 1 (sh3rf1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018093030.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTTCTTT CCATGCAAAA CTGTGTCGAT AAAACCATGG ATGAGTCAGC ATTACTGGAC 
    CTCTTAGAAT GTCCAGTGTG TCTTGAGAGA CTTGATGCCT CTGCTAAAGT CTTGCCATGC
    CAACACACAT TCTGCAAGCG CTGCCTTCTG GGCATTGTGA GCTCTCGCAA TGAGCTGCGC
    TGCCCAGAGT GCAGGACTCT GGTGGAGTGT GGGGTGGATG AACTTCCGAG CAACATCCTA
    CTAGTGAGAC TGTTGGATGG AATAAAGCAG AGACCAAGAA AAGCAGGGGT TGGTGGCAGT
    GCTGGGAATA GCACAAATGT GCTAAGAGCA CAAGGCAGTC TAACTACTAA TTGTGGCTTA
    AATGATGCAC AGAATATTCA TGGTGGGCAA CAGAGGATAC AGGCACGAAG TCCTCCAGTA
    AGGGGTGTTC CTCAGTTACC ATGTGCCAAA GCTTTGTACA ACTACGAGGG GAAAGAGCCT
    GGAGATCTGA AATTTAACAA GGGAGACATC ATCGTGCTTC GCCGTCAGGT TGATGAAAAT
    TGGTACCACG GGGAGATTAA TGGCATCCAT GGCTTCTTCC CAACAAACTT TGTGCAGATC
    ATAAAGCCTT TACCTCAGCC TCCTCCTCAA TGCAAAGCAC TTTATGACTT TGAAGTGAAA
    GACAAGGAAG CAGACAAGGA TTGCCTACCC TTTTTAAAGG ATGACATTCT CACCGTGATC
    CGACGAGTAG ATGAAAACTG GGCAGAAGGA ATGCTGGGAG ACAAGATAGG AATATTCCCT
    ATTTCCTATG TAGAGTTCAA TTCAGCAGCC AAGCAGCTGA TAGAATTGGA CAAGCCATCA
    GGAGTAGACA CTGGGGAAGG CTCTTCTGGC ACAACGCATA GTAGCAATTC TCAAAAGCAA
    GCAGATGCAA AGAAGAACAC CAAAAAGCGT CACTCCTTTA CCTCCCTCAC CATGTCTAAC
    AAGTCATCGC AGTCCGTGCA GAATCGTCAT TCAATGGAGA TTAGCCCCCC TGTACTCATC
    AGTTCTAGCA ATCCCACCGC TGCTGCCAGG ATCAGTGAAC TCACAGGCCT CTCTTGTAGT
    GCCCCTTCTC AGGTTCATAT CAGTACAACA GGGCTCATTG TGACCCCACC CCCTAGCAGC
    CCTGTTGTAA GTGGCCCTGC ATTTACATTT CCACCGGAAG TCACCTACCA AGCTGCTCTG
    GGAGATTTAA ATCCTCCTCT TCTTCCTCCT CCACCATTGG CCACTCCGGT TATCACGTCG
    ACATCTTCAG GAGCTGCTGC CGCTGTCCAG AGGTCTATCA GCGGTCCAGC TGAGCAAGTC
    ACACACTTGA GGACATCTAC AAGACCCAGT GTGGCATTAA CAACTGCAAC ACCAGCAAAG
    GTTGCCATGG CCACAGCTAG TAGCAGTAAT GTGGTGAACT TAGTAACGCC AACACCTCCA
    GGGGCTCCAT GCCAAAAACT GCAAGGGAAT GGAGCGGAGT TTGCCAAGAC TGTCAGTACA
    AATGGGGTCC CACCGGCTGG CATTCCTGGT TCTCATATCC AGAGTAGCCC ACAGGCCAAG
    GTTCTGCTGC ACATGAGTGG CCAAATGACC GTTAACCAAG CCCGCAATGC GGTCCGAACA
    GCTGCTGCAC ACAGCCAGGA TCGTCCAACA GCAGCTGTGA CTCCCATCCA GGCTCAGATC
    CCATCTGCAT CTGTTCTCCC CCAGCAGGCT GCCACTTCAC AGCAAATGCC ACCTCCGCTT
    TCAGGTCCAG CTGCTTACAT CAATGCTGCA ATGAACATTA GCCGACCAAG TGTGCCGGTG
    GCTTCGGCAG CCTCCTCCAG TGTTTCCTCC GCTGCATTTG AAACAGAATG TAATTGGAAA
    TCTGGCTCTG GACTTGCTGC GTGTTCTTTT CCAGAAAATG TCTCTGCACC TTTAAATTCT
    GCTGCTAATA AGCAAGATAA AGATAGTAAG AAAGAAAAGA AGGGCTTGCT CAAGTTACTG
    TCTGGGGCAT CTACAAAAAG AAAGCCTCGC TCATCTCCGC CGCACTCTCC AACCCAAGAA
    CTGGAACAGA CAAATTCAGA AGCAGCGCTG GAGGGAGCTG TAGGGCCAGA CATTCTGCCT
    GTCAATGGCA ATGGCCGAGT TGCTTCCTGC ACTGTGGATT GTGATTTAGT ATCTGCCTCA
    GCTCTGGTAC AGGACAACCG CAAGCCAGCT TCCTTGGATA CCAATGTTCC CATTGCACCT
    CCACCAAGAC AGCCTTGCTC CTCACTGGGC ACTGTTCTGA ATGACTCCCG GCCTTGTGAA
    AGATATAGAG TTGTAGTGTC CTACCCACCC CAGAGCGAGG CAGAACTAGA GCTGAAGGAA
    GGGGACATCG TATTTGTGCA CAAGAAACGG GAAGATGGTT GGTTTAAAGG CACACTCCAA
    CGGAACGGCA AAACCGGGCT ATTTCCCGGC AGCTTTGTGG AGAATATTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.