nsun2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	nsun2
Entrez Gene ID	548716
Full Name	NOP2/Sun RNA methyltransferase family member 2
General protein information	Preferred Names NOP2/Sun RNA methyltransferase family member 2 Names tRNA (cytosine(34)-C(5))-methyltransferase NOL1/NOP2/Sun domain family, member 2 NOP2/Sun domain family, member 2 tRNA (cytosine-5-)-methyltransferase NSUN2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012964665.2	XP_012820119.1	tRNA (cytosine(34)-C(5))-methyltransferase isoform X1
NM_001015962.2	NP_001015962.1	RNA cytosine-C(5)-methyltransferase NSUN2
XM_012964666.3	XP_012820120.1	RNA cytosine-C(5)-methyltransferase NSUN2 isoform X1
XM_012964666.2	XP_012820120.1	tRNA (cytosine(34)-C(5))-methyltransferase isoform X1
XM_012964665.3	XP_012820119.1	RNA cytosine-C(5)-methyltransferase NSUN2 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	NSUN2	NP_001026175.1
Oryza sativa (rice)	Os08g0484400	NP_001062081.1
Canis lupus familiaris (dog)	NSUN2	XP_535800.2
Schizosaccharomyces pombe (fission yeast)	SPAC17D4.04	NP_594695.3
Neurospora crassa	NCU05301	XP_962072.1
Arabidopsis thaliana (thale cress)	AT4G40000	NP_195710.3
Oryza sativa (rice)	Os09g0471900	NP_001063445.1
Pan troglodytes (chimpanzee)	NSUN2	XP_517620.2
Mus musculus (house mouse)	Nsun2	NP_663329.3
Drosophila melanogaster (fruit fly)	Nsun2	NP_652007.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002504	XP_563662.3
Caenorhabditis elegans (roundworm)	Y48G8AL.5	NP_490830.2
Eremothecium gossypii	AGOS_AFR179C	NP_985726.2
Homo sapiens (human)	NSUN2	NP_060225.4
Saccharomyces cerevisiae (baker's yeast)	NCL1	NP_009529.1
Kluyveromyces lactis	KLLA0F12562g	XP_455648.1
	NSUN2	XP_002804365.1
Bos taurus (cattle)	NSUN2	XP_002696495.1
Rattus norvegicus (Norway rat)	Nsun2	NP_001101873.1
Danio rerio (zebrafish)	nsun2	NP_956005.1
Schizosaccharomyces pombe (fission yeast)	SPAC23C4.17	NP_593189.2
Magnaporthe oryzae (rice blast fungus)	MGG_13217	XP_003711942.1
Arabidopsis thaliana (thale cress)	AT2G22400	NP_850024.1
Xenopus tropicalis (tropical clawed frog)	nsun2	NP_001015962.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nsun2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nsun2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa25768	NM_001015962.2 Latest version!	Xenopus tropicalis NOP2/Sun RNA methyltransferase 2 (nsun2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa25768	XM_012964665.3 Latest version!	Xenopus tropicalis NOP2/Sun RNA methyltransferase 2 (nsun2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa25768	XM_012964666.3 Latest version!	Xenopus tropicalis NOP2/Sun RNA methyltransferase 2 (nsun2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OXa25768	XM_012964666.2	Xenopus tropicalis NOP2/Sun RNA methyltransferase family member 2 (nsun2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa25768	XM_012964665.2	Xenopus tropicalis NOP2/Sun RNA methyltransferase family member 2 (nsun2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nsun2 ( NM_001015962.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001015962.1 Xenopus tropicalis NOP2/Sun RNA methyltransferase 2 (nsun2), mRNA.

Price & Availability↑

CloneID	OXa25768
Clone ID Related Accession (Same CDS sequence)	XM_012964665.2 , NM_001015962.2 , XM_012964666.3 , XM_012964666.2 , XM_012964665.3
Accession Version	NM_001015962.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Xenopus tropicalis(tropical clawed frog)
Product	RNA cytosine-C(5)-methyltransferase NSUN2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR848434.2. On Oct 14, 2005 this sequence version replaced NM_001015962.1. ##Evidence-Data-START## Transcript exon combination :: CR848434.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGGCCGCA GAAATCGGCG AAACCGCCAG AGGCACCAAA GGAGCACTGA GCAGCGAAGT 
CCCGCAGAGG AAGAGCAGCG GCGCAAAGCT AGAGAGCAGG CGGCTTGGGA ATGTGGTTAC 
CCTGAAATCA TCAAAGAAAA CAAATTATTT GAACATTATT ATCAGGAACT AAAGATTGTA 
CCTGATGGGG AGTGGGACAA GTTCATGGCT TCTCTCCGGG AACCCCTTCC GGCTACCATA 
AGAATCACAG GATACAAAAG CCATGCTAAA GAAATACTGC ATTGTCTGAA GGAGAAATAC 
TTCAAAGAAC TGCAAGATAT TGAGGTTGAT GGGCAGAAGA TAGAAGCTCC ACAGCCATTA 
AGCTGGTACC CTGAAGAGCT TGCCTGGCAT ACCAATCTGA GCAGGAAAAT AATTAGAAAG 
TCTCCAGAAC TGGAAAAGTT TCATCAGTTT CTTGTCAACG AAACAGAATC GGGCAACATT 
AGTCGGCAAG AGGCTGTTAG TATGATTCCA CCAGTCCTTC TGAAAGTTCA GCCGCATCAC 
AAGATTCTGG ATATGTGTGC TGCTCCTGGA TCAAAAACAG CCCAGATAAT TGAAATGTTA 
CATGCCGATA TGAATGTACC CTTTCCTGAG GGTTTTGTCA TTGCAAATGA TGTTGACAAT 
AAACGCTGTT ACTTACTGGT TCATCAAGCC AAGAGACTAA ACAGTCCTTG CATTATGGTA 
GTAAACCATG ATGCTTCCAG CATTCCCAGA CTACTAGTAG AGAACAATGG TTCTAGAGAG 
GTGTTGTACT ACGACAGAAT ACTCTGTGAT GTACCATGCA GCGGTGATGG GACTTTGAGG 
AAAAACATTG ACGTGTGGAA GAAGTGGACA ACTTTGAACA GCTTGCAGTT ACATGGGCTA 
CAGATTAGGA TTGCTACCCG TGGGGTAGAA CAGTTAGCAG AAGGGGGCCG AATGGTCTAT 
TCTACGTGCT CTTTGAATCC TGTTGAAGAT GAGGCTGTTA TAGCTTCTCT GTTGGACAAA 
AGTGAAGGTT CCCTGGAGCT TGCTGACGTG GCTTCAGAAA TTCCTGGCTT AAAATGGATG 
CCTGGGATTA CCCAGTGGAA GGTAATGACA AAAGAAGGAC ACTGGTATGA GAAATGGGAA 
GATATTCCCA CAAGCAGACA TACTCAGATT CGCCCAACAA TGTTTCCGCC AAAAGATGAG 
GAGAAATTGA AATCAATGAA TCTAAACCGT TGCATGCGAA TACTACCGCA TCATCAAAAC 
ACAGGAGGAT TCTTTGTAGC TGTGTTGATA AAGAAAGCAC CAATGCCATG GAACAAACGA 
CAGCCAAAGC TGCAAAGGAG ACCTCCTGTT TCAGCTTGTG ATGCATCTAT TGCTGTTGCC 
CCCGAGCTTG TGAAAGCTGT CACTGAGAAT AGTGCAGGAA TGGCAGATGA ACCTGCTGTA 
GACACAGAAA ATGGTGAAAC TAAACCTTGT ACAAACCAGA GTGATTCTAG TAAAACTGAT 
ATTGTTTGCT GCCCACCTCC TTCCAAAAAA ATGAAGTTGT TTGGTTTTAA AGAAGATCCT 
TTTGTCTTTG TTTCTGAAGA TGATCCAATT TTTGACCCAA TCCAGACGTT CTATGCACTT 
GATCCTTCCT TCCCTAAAAA GAACTTACTG ACACGAACAC AGGAGGGAAA AAAAAGGCAG 
TTGTATATGG TCTCTAAGGA GCTGCGAAAT GTACTTCTCC ATAATAGTGA GAAGATGAAG 
GTAATTAACA CTGGTATAAA GGTGTTATGC CGCAACAATG ATGGAGAACA GTATGGTTGT 
GCTTACAGAT TAGCACAAGA GGGCATATAT ACACTTTATC CGTTCATCAA TGCGAGAATT 
GTAACTGTAT CTATCGAAGA TATCAAAGTT CTACTCACCC AAGAAAATCC TTTCCTTAGT 
AAATTTAGTA AAGAAACACA GAAACAAGCC AATAATTTTG ATATGGGAAG CATTGTCCTT 
AAATATGAAC CTGATCCACA AGAACCGGAG ACTCTGCAGT GCCCCATTGT TCTTTGTGGC 
TGGCGTGGGA AAACATCGAT CCGTTCTTTT GTACCGAAAA ACGAAAGATT ACATTATCTA 
AGAATGATGG GAGTAGAAGT GTTTAAGGAG AAAGCTGAAG TCCTTGAAAA AAAGCCTGTA 
GAAGGAAAGG CATGCGATGA GGAGCATATA GATGAGAAAA TGGACATTGA CGGTGCAAAG 
GAGGAGAGTA AAGAATTGTC GGGAAATGAG AGTGGTGATG ATGAGGACCC TAAGGAAGAA 
GACGTTATTG ACAGAGGAGT CTTGGAACAT GTAGCACTTA AAAACACCTC AGCAATCCCA 
GCATCTGTCG AAGATCAAGC TGAGGATGCC AGCGTGAGCA AAGAGTCAGT AGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001015962.1
CDS	128..2524
Translation	MGRRNRRNRQRHQRSTEQRSPAEEEQRRKAREQAAWECGYPEIIKENKLFEHYYQELKIVPDGEWDKFMASLREPLPATIRITGYKSHAKEILHCLKEKYFKELQDIEVDGQKIEAPQPLSWYPEELAWHTNLSRKIIRKSPELEKFHQFLVNETESGNISRQEAVSMIPPVLLKVQPHHKILDMCAAPGSKTAQIIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLNSPCIMVVNHDASSIPRLLVENNGSREVLYYDRILCDVPCSGDGTLRKNIDVWKKWTTLNSLQLHGLQIRIATRGVEQLAEGGRMVYSTCSLNPVEDEAVIASLLDKSEGSLELADVASEIPGLKWMPGITQWKVMTKEGHWYEKWEDIPTSRHTQIRPTMFPPKDEEKLKSMNLNRCMRILPHHQNTGGFFVAVLIKKAPMPWNKRQPKLQRRPPVSACDASIAVAPELVKAVTENSAGMADEPAVDTENGETKPCTNQSDSSKTDIVCCPPPSKKMKLFGFKEDPFVFVSEDDPIFDPIQTFYALDPSFPKKNLLTRTQEGKKRQLYMVSKELRNVLLHNSEKMKVINTGIKVLCRNNDGEQYGCAYRLAQEGIYTLYPFINARIVTVSIEDIKVLLTQENPFLSKFSKETQKQANNFDMGSIVLKYEPDPQEPETLQCPIVLCGWRGKTSIRSFVPKNERLHYLRMMGVEVFKEKAEVLEKKPVEGKACDEEHIDEKMDIDGAKEESKELSGNESGDDEDPKEEDVIDRGVLEHVALKNTSAIPASVEDQAEDASVSKESVD

Target ORF information:

RefSeq Version	NM_001015962.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NOP2/Sun RNA methyltransferase 2 (nsun2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001015962.2

ORF Insert Sequence:

ATGGGCCGCA GAAATCGGCG AAACCGCCAG AGGCACCAAA GGAGCACTGA GCAGCGAAGT 
CCCGCAGAGG AAGAGCAGCG GCGCAAAGCT AGAGAGCAGG CGGCTTGGGA ATGTGGTTAC 
CCTGAAATCA TCAAAGAAAA CAAATTATTT GAACATTATT ATCAGGAACT AAAGATTGTA 
CCTGATGGGG AGTGGGACAA GTTCATGGCT TCTCTCCGGG AACCCCTTCC GGCTACCATA 
AGAATCACAG GATACAAAAG CCATGCTAAA GAAATACTGC ATTGTCTGAA GGAGAAATAC 
TTCAAAGAAC TGCAAGATAT TGAGGTTGAT GGGCAGAAGA TAGAAGCTCC ACAGCCATTA 
AGCTGGTACC CTGAAGAGCT TGCCTGGCAT ACCAATCTGA GCAGGAAAAT AATTAGAAAG 
TCTCCAGAAC TGGAAAAGTT TCATCAGTTT CTTGTCAACG AAACAGAATC GGGCAACATT 
AGTCGGCAAG AGGCTGTTAG TATGATTCCA CCAGTCCTTC TGAAAGTTCA GCCGCATCAC 
AAGATTCTGG ATATGTGTGC TGCTCCTGGA TCAAAAACAG CCCAGATAAT TGAAATGTTA 
CATGCCGATA TGAATGTACC CTTTCCTGAG GGTTTTGTCA TTGCAAATGA TGTTGACAAT 
AAACGCTGTT ACTTACTGGT TCATCAAGCC AAGAGACTAA ACAGTCCTTG CATTATGGTA 
GTAAACCATG ATGCTTCCAG CATTCCCAGA CTACTAGTAG AGAACAATGG TTCTAGAGAG 
GTGTTGTACT ACGACAGAAT ACTCTGTGAT GTACCATGCA GCGGTGATGG GACTTTGAGG 
AAAAACATTG ACGTGTGGAA GAAGTGGACA ACTTTGAACA GCTTGCAGTT ACATGGGCTA 
CAGATTAGGA TTGCTACCCG TGGGGTAGAA CAGTTAGCAG AAGGGGGCCG AATGGTCTAT 
TCTACGTGCT CTTTGAATCC TGTTGAAGAT GAGGCTGTTA TAGCTTCTCT GTTGGACAAA 
AGTGAAGGTT CCCTGGAGCT TGCTGACGTG GCTTCAGAAA TTCCTGGCTT AAAATGGATG 
CCTGGGATTA CCCAGTGGAA GGTAATGACA AAAGAAGGAC ACTGGTATGA GAAATGGGAA 
GATATTCCCA CAAGCAGACA TACTCAGATT CGCCCAACAA TGTTTCCGCC AAAAGATGAG 
GAGAAATTGA AATCAATGAA TCTAAACCGT TGCATGCGAA TACTACCGCA TCATCAAAAC 
ACAGGAGGAT TCTTTGTAGC TGTGTTGATA AAGAAAGCAC CAATGCCATG GAACAAACGA 
CAGCCAAAGC TGCAAAGGAG ACCTCCTGTT TCAGCTTGTG ATGCATCTAT TGCTGTTGCC 
CCCGAGCTTG TGAAAGCTGT CACTGAGAAT AGTGCAGGAA TGGCAGATGA ACCTGCTGTA 
GACACAGAAA ATGGTGAAAC TAAACCTTGT ACAAACCAGA GTGATTCTAG TAAAACTGAT 
ATTGTTTGCT GCCCACCTCC TTCCAAAAAA ATGAAGTTGT TTGGTTTTAA AGAAGATCCT 
TTTGTCTTTG TTTCTGAAGA TGATCCAATT TTTGACCCAA TCCAGACGTT CTATGCACTT 
GATCCTTCCT TCCCTAAAAA GAACTTACTG ACACGAACAC AGGAGGGAAA AAAAAGGCAG 
TTGTATATGG TCTCTAAGGA GCTGCGAAAT GTACTTCTCC ATAATAGTGA GAAGATGAAG 
GTAATTAACA CTGGTATAAA GGTGTTATGC CGCAACAATG ATGGAGAACA GTATGGTTGT 
GCTTACAGAT TAGCACAAGA GGGCATATAT ACACTTTATC CGTTCATCAA TGCGAGAATT 
GTAACTGTAT CTATCGAAGA TATCAAAGTT CTACTCACCC AAGAAAATCC TTTCCTTAGT 
AAATTTAGTA AAGAAACACA GAAACAAGCC AATAATTTTG ATATGGGAAG CATTGTCCTT 
AAATATGAAC CTGATCCACA AGAACCGGAG ACTCTGCAGT GCCCCATTGT TCTTTGTGGC 
TGGCGTGGGA AAACATCGAT CCGTTCTTTT GTACCGAAAA ACGAAAGATT ACATTATCTA 
AGAATGATGG GAGTAGAAGT GTTTAAGGAG AAAGCTGAAG TCCTTGAAAA AAAGCCTGTA 
GAAGGAAAGG CATGCGATGA GGAGCATATA GATGAGAAAA TGGACATTGA CGGTGCAAAG 
GAGGAGAGTA AAGAATTGTC GGGAAATGAG AGTGGTGATG ATGAGGACCC TAAGGAAGAA 
GACGTTATTG ACAGAGGAGT CTTGGAACAT GTAGCACTTA AAAACACCTC AGCAATCCCA 
GCATCTGTCG AAGATCAAGC TGAGGATGCC AGCGTGAGCA AAGAGTCAGT AGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsun2 ( XM_012964665.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820119.1 Xenopus tropicalis NOP2/Sun RNA methyltransferase family member 2 (nsun2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa25768
Clone ID Related Accession (Same CDS sequence)	XM_012964665.2 , NM_001015962.2 , XM_012964666.3 , XM_012964666.2 , XM_012964665.3
Accession Version	XM_012964665.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tRNA (cytosine(34)-C(5))-methyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964665.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCGCA GAAATCGGCG AAACCGCCAG AGGCACCAAA GGAGCACTGA GCAGCGAAGT 
CCCGCAGAGG AAGAGCAGCG GCGCAAAGCT AGAGAGCAGG CGGCTTGGGA ATGTGGTTAC 
CCTGAAATCA TCAAAGAAAA CAAATTATTT GAACATTATT ATCAGGAACT AAAGATTGTA 
CCTGATGGGG AGTGGGACAA GTTCATGGCT TCTCTCCGGG AACCCCTTCC GGCTACCATA 
AGAATCACAG GATACAAAAG CCATGCTAAA GAAATACTGC ATTGTCTGAA GGAGAAATAC 
TTCAAAGAAC TGCAAGATAT TGAGGTTGAT GGGCAGAAGA TAGAAGCTCC ACAGCCATTA 
AGCTGGTACC CTGAAGAGCT TGCCTGGCAT ACCAATCTGA GCAGGAAAAT AATTAGAAAG 
TCTCCAGAAC TGGAAAAGTT TCATCAGTTT CTTGTCAACG AAACAGAATC GGGCAACATT 
AGTCGGCAAG AGGCTGTTAG TATGATTCCA CCAGTCCTTC TGAAAGTTCA GCCGCATCAC 
AAGATTCTGG ATATGTGTGC TGCTCCTGGA TCAAAAACAG CCCAGATAAT TGAAATGTTA 
CATGCCGATA TGAATGTACC CTTTCCTGAG GGTTTTGTCA TTGCAAATGA TGTTGACAAT 
AAACGCTGTT ACTTACTGGT TCATCAAGCC AAGAGACTAA ACAGTCCTTG CATTATGGTA 
GTAAACCATG ATGCTTCCAG CATTCCCAGA CTACTAGTAG AGAACAATGG TTCTAGAGAG 
GTGTTGTACT ACGACAGAAT ACTCTGTGAT GTACCATGCA GCGGTGATGG GACTTTGAGG 
AAAAACATTG ACGTGTGGAA GAAGTGGACA ACTTTGAACA GCTTGCAGTT ACATGGGCTA 
CAGATTAGGA TTGCTACCCG TGGGGTAGAA CAGTTAGCAG AAGGGGGCCG AATGGTCTAT 
TCTACGTGCT CTTTGAATCC TGTTGAAGAT GAGGCTGTTA TAGCTTCTCT GTTGGACAAA 
AGTGAAGGTT CCCTGGAGCT TGCTGACGTG GCTTCAGAAA TTCCTGGCTT AAAATGGATG 
CCTGGGATTA CCCAGTGGAA GGTAATGACA AAAGAAGGAC ACTGGTATGA GAAATGGGAA 
GATATTCCCA CAAGCAGACA TACTCAGATT CGCCCAACAA TGTTTCCGCC AAAAGATGAG 
GAGAAATTGA AATCAATGAA TCTAAACCGT TGCATGCGAA TACTACCGCA TCATCAAAAC 
ACAGGAGGAT TCTTTGTAGC TGTGTTGATA AAGAAAGCAC CAATGCCATG GAACAAACGA 
CAGCCAAAGC TGCAAAGGAG ACCTCCTGTT TCAGCTTGTG ATGCATCTAT TGCTGTTGCC 
CCCGAGCTTG TGAAAGCTGT CACTGAGAAT AGTGCAGGAA TGGCAGATGA ACCTGCTGTA 
GACACAGAAA ATGGTGAAAC TAAACCTTGT ACAAACCAGA GTGATTCTAG TAAAACTGAT 
ATTGTTTGCT GCCCACCTCC TTCCAAAAAA ATGAAGTTGT TTGGTTTTAA AGAAGATCCT 
TTTGTCTTTG TTTCTGAAGA TGATCCAATT TTTGACCCAA TCCAGACGTT CTATGCACTT 
GATCCTTCCT TCCCTAAAAA GAACTTACTG ACACGAACAC AGGAGGGAAA AAAAAGGCAG 
TTGTATATGG TCTCTAAGGA GCTGCGAAAT GTACTTCTCC ATAATAGTGA GAAGATGAAG 
GTAATTAACA CTGGTATAAA GGTGTTATGC CGCAACAATG ATGGAGAACA GTATGGTTGT 
GCTTACAGAT TAGCACAAGA GGGCATATAT ACACTTTATC CGTTCATCAA TGCGAGAATT 
GTAACTGTAT CTATCGAAGA TATCAAAGTT CTACTCACCC AAGAAAATCC TTTCCTTAGT 
AAATTTAGTA AAGAAACACA GAAACAAGCC AATAATTTTG ATATGGGAAG CATTGTCCTT 
AAATATGAAC CTGATCCACA AGAACCGGAG ACTCTGCAGT GCCCCATTGT TCTTTGTGGC 
TGGCGTGGGA AAACATCGAT CCGTTCTTTT GTACCGAAAA ACGAAAGATT ACATTATCTA 
AGAATGATGG GAGTAGAAGT GTTTAAGGAG AAAGCTGAAG TCCTTGAAAA AAAGCCTGTA 
GAAGGAAAGG CATGCGATGA GGAGCATATA GATGAGAAAA TGGACATTGA CGGTGCAAAG 
GAGGAGAGTA AAGAATTGTC GGGAAATGAG AGTGGTGATG ATGAGGACCC TAAGGAAGAA 
GACGTTATTG ACAGAGGAGT CTTGGAACAT GTAGCACTTA AAAACACCTC AGCAATCCCA 
GCATCTGTCG AAGATCAAGC TGAGGATGCC AGCGTGAGCA AAGAGTCAGT AGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820119.1
CDS	190..2586
Translation	MGRRNRRNRQRHQRSTEQRSPAEEEQRRKAREQAAWECGYPEIIKENKLFEHYYQELKIVPDGEWDKFMASLREPLPATIRITGYKSHAKEILHCLKEKYFKELQDIEVDGQKIEAPQPLSWYPEELAWHTNLSRKIIRKSPELEKFHQFLVNETESGNISRQEAVSMIPPVLLKVQPHHKILDMCAAPGSKTAQIIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLNSPCIMVVNHDASSIPRLLVENNGSREVLYYDRILCDVPCSGDGTLRKNIDVWKKWTTLNSLQLHGLQIRIATRGVEQLAEGGRMVYSTCSLNPVEDEAVIASLLDKSEGSLELADVASEIPGLKWMPGITQWKVMTKEGHWYEKWEDIPTSRHTQIRPTMFPPKDEEKLKSMNLNRCMRILPHHQNTGGFFVAVLIKKAPMPWNKRQPKLQRRPPVSACDASIAVAPELVKAVTENSAGMADEPAVDTENGETKPCTNQSDSSKTDIVCCPPPSKKMKLFGFKEDPFVFVSEDDPIFDPIQTFYALDPSFPKKNLLTRTQEGKKRQLYMVSKELRNVLLHNSEKMKVINTGIKVLCRNNDGEQYGCAYRLAQEGIYTLYPFINARIVTVSIEDIKVLLTQENPFLSKFSKETQKQANNFDMGSIVLKYEPDPQEPETLQCPIVLCGWRGKTSIRSFVPKNERLHYLRMMGVEVFKEKAEVLEKKPVEGKACDEEHIDEKMDIDGAKEESKELSGNESGDDEDPKEEDVIDRGVLEHVALKNTSAIPASVEDQAEDASVSKESVD

Target ORF information:

RefSeq Version	XM_012964665.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NOP2/Sun RNA methyltransferase family member 2 (nsun2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964665.2

ORF Insert Sequence:

ATGGGCCGCA GAAATCGGCG AAACCGCCAG AGGCACCAAA GGAGCACTGA GCAGCGAAGT 
CCCGCAGAGG AAGAGCAGCG GCGCAAAGCT AGAGAGCAGG CGGCTTGGGA ATGTGGTTAC 
CCTGAAATCA TCAAAGAAAA CAAATTATTT GAACATTATT ATCAGGAACT AAAGATTGTA 
CCTGATGGGG AGTGGGACAA GTTCATGGCT TCTCTCCGGG AACCCCTTCC GGCTACCATA 
AGAATCACAG GATACAAAAG CCATGCTAAA GAAATACTGC ATTGTCTGAA GGAGAAATAC 
TTCAAAGAAC TGCAAGATAT TGAGGTTGAT GGGCAGAAGA TAGAAGCTCC ACAGCCATTA 
AGCTGGTACC CTGAAGAGCT TGCCTGGCAT ACCAATCTGA GCAGGAAAAT AATTAGAAAG 
TCTCCAGAAC TGGAAAAGTT TCATCAGTTT CTTGTCAACG AAACAGAATC GGGCAACATT 
AGTCGGCAAG AGGCTGTTAG TATGATTCCA CCAGTCCTTC TGAAAGTTCA GCCGCATCAC 
AAGATTCTGG ATATGTGTGC TGCTCCTGGA TCAAAAACAG CCCAGATAAT TGAAATGTTA 
CATGCCGATA TGAATGTACC CTTTCCTGAG GGTTTTGTCA TTGCAAATGA TGTTGACAAT 
AAACGCTGTT ACTTACTGGT TCATCAAGCC AAGAGACTAA ACAGTCCTTG CATTATGGTA 
GTAAACCATG ATGCTTCCAG CATTCCCAGA CTACTAGTAG AGAACAATGG TTCTAGAGAG 
GTGTTGTACT ACGACAGAAT ACTCTGTGAT GTACCATGCA GCGGTGATGG GACTTTGAGG 
AAAAACATTG ACGTGTGGAA GAAGTGGACA ACTTTGAACA GCTTGCAGTT ACATGGGCTA 
CAGATTAGGA TTGCTACCCG TGGGGTAGAA CAGTTAGCAG AAGGGGGCCG AATGGTCTAT 
TCTACGTGCT CTTTGAATCC TGTTGAAGAT GAGGCTGTTA TAGCTTCTCT GTTGGACAAA 
AGTGAAGGTT CCCTGGAGCT TGCTGACGTG GCTTCAGAAA TTCCTGGCTT AAAATGGATG 
CCTGGGATTA CCCAGTGGAA GGTAATGACA AAAGAAGGAC ACTGGTATGA GAAATGGGAA 
GATATTCCCA CAAGCAGACA TACTCAGATT CGCCCAACAA TGTTTCCGCC AAAAGATGAG 
GAGAAATTGA AATCAATGAA TCTAAACCGT TGCATGCGAA TACTACCGCA TCATCAAAAC 
ACAGGAGGAT TCTTTGTAGC TGTGTTGATA AAGAAAGCAC CAATGCCATG GAACAAACGA 
CAGCCAAAGC TGCAAAGGAG ACCTCCTGTT TCAGCTTGTG ATGCATCTAT TGCTGTTGCC 
CCCGAGCTTG TGAAAGCTGT CACTGAGAAT AGTGCAGGAA TGGCAGATGA ACCTGCTGTA 
GACACAGAAA ATGGTGAAAC TAAACCTTGT ACAAACCAGA GTGATTCTAG TAAAACTGAT 
ATTGTTTGCT GCCCACCTCC TTCCAAAAAA ATGAAGTTGT TTGGTTTTAA AGAAGATCCT 
TTTGTCTTTG TTTCTGAAGA TGATCCAATT TTTGACCCAA TCCAGACGTT CTATGCACTT 
GATCCTTCCT TCCCTAAAAA GAACTTACTG ACACGAACAC AGGAGGGAAA AAAAAGGCAG 
TTGTATATGG TCTCTAAGGA GCTGCGAAAT GTACTTCTCC ATAATAGTGA GAAGATGAAG 
GTAATTAACA CTGGTATAAA GGTGTTATGC CGCAACAATG ATGGAGAACA GTATGGTTGT 
GCTTACAGAT TAGCACAAGA GGGCATATAT ACACTTTATC CGTTCATCAA TGCGAGAATT 
GTAACTGTAT CTATCGAAGA TATCAAAGTT CTACTCACCC AAGAAAATCC TTTCCTTAGT 
AAATTTAGTA AAGAAACACA GAAACAAGCC AATAATTTTG ATATGGGAAG CATTGTCCTT 
AAATATGAAC CTGATCCACA AGAACCGGAG ACTCTGCAGT GCCCCATTGT TCTTTGTGGC 
TGGCGTGGGA AAACATCGAT CCGTTCTTTT GTACCGAAAA ACGAAAGATT ACATTATCTA 
AGAATGATGG GAGTAGAAGT GTTTAAGGAG AAAGCTGAAG TCCTTGAAAA AAAGCCTGTA 
GAAGGAAAGG CATGCGATGA GGAGCATATA GATGAGAAAA TGGACATTGA CGGTGCAAAG 
GAGGAGAGTA AAGAATTGTC GGGAAATGAG AGTGGTGATG ATGAGGACCC TAAGGAAGAA 
GACGTTATTG ACAGAGGAGT CTTGGAACAT GTAGCACTTA AAAACACCTC AGCAATCCCA 
GCATCTGTCG AAGATCAAGC TGAGGATGCC AGCGTGAGCA AAGAGTCAGT AGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsun2 ( XM_012964666.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820120.1 Xenopus tropicalis NOP2/Sun RNA methyltransferase family member 2 (nsun2), transcript variant X2, mRNA.

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CloneID	OXa25768
Clone ID Related Accession (Same CDS sequence)	XM_012964665.2 , NM_001015962.2 , XM_012964666.3 , XM_012964666.2 , XM_012964665.3
Accession Version	XM_012964666.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tRNA (cytosine(34)-C(5))-methyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964666.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCGCA GAAATCGGCG AAACCGCCAG AGGCACCAAA GGAGCACTGA GCAGCGAAGT 
CCCGCAGAGG AAGAGCAGCG GCGCAAAGCT AGAGAGCAGG CGGCTTGGGA ATGTGGTTAC 
CCTGAAATCA TCAAAGAAAA CAAATTATTT GAACATTATT ATCAGGAACT AAAGATTGTA 
CCTGATGGGG AGTGGGACAA GTTCATGGCT TCTCTCCGGG AACCCCTTCC GGCTACCATA 
AGAATCACAG GATACAAAAG CCATGCTAAA GAAATACTGC ATTGTCTGAA GGAGAAATAC 
TTCAAAGAAC TGCAAGATAT TGAGGTTGAT GGGCAGAAGA TAGAAGCTCC ACAGCCATTA 
AGCTGGTACC CTGAAGAGCT TGCCTGGCAT ACCAATCTGA GCAGGAAAAT AATTAGAAAG 
TCTCCAGAAC TGGAAAAGTT TCATCAGTTT CTTGTCAACG AAACAGAATC GGGCAACATT 
AGTCGGCAAG AGGCTGTTAG TATGATTCCA CCAGTCCTTC TGAAAGTTCA GCCGCATCAC 
AAGATTCTGG ATATGTGTGC TGCTCCTGGA TCAAAAACAG CCCAGATAAT TGAAATGTTA 
CATGCCGATA TGAATGTACC CTTTCCTGAG GGTTTTGTCA TTGCAAATGA TGTTGACAAT 
AAACGCTGTT ACTTACTGGT TCATCAAGCC AAGAGACTAA ACAGTCCTTG CATTATGGTA 
GTAAACCATG ATGCTTCCAG CATTCCCAGA CTACTAGTAG AGAACAATGG TTCTAGAGAG 
GTGTTGTACT ACGACAGAAT ACTCTGTGAT GTACCATGCA GCGGTGATGG GACTTTGAGG 
AAAAACATTG ACGTGTGGAA GAAGTGGACA ACTTTGAACA GCTTGCAGTT ACATGGGCTA 
CAGATTAGGA TTGCTACCCG TGGGGTAGAA CAGTTAGCAG AAGGGGGCCG AATGGTCTAT 
TCTACGTGCT CTTTGAATCC TGTTGAAGAT GAGGCTGTTA TAGCTTCTCT GTTGGACAAA 
AGTGAAGGTT CCCTGGAGCT TGCTGACGTG GCTTCAGAAA TTCCTGGCTT AAAATGGATG 
CCTGGGATTA CCCAGTGGAA GGTAATGACA AAAGAAGGAC ACTGGTATGA GAAATGGGAA 
GATATTCCCA CAAGCAGACA TACTCAGATT CGCCCAACAA TGTTTCCGCC AAAAGATGAG 
GAGAAATTGA AATCAATGAA TCTAAACCGT TGCATGCGAA TACTACCGCA TCATCAAAAC 
ACAGGAGGAT TCTTTGTAGC TGTGTTGATA AAGAAAGCAC CAATGCCATG GAACAAACGA 
CAGCCAAAGC TGCAAAGGAG ACCTCCTGTT TCAGCTTGTG ATGCATCTAT TGCTGTTGCC 
CCCGAGCTTG TGAAAGCTGT CACTGAGAAT AGTGCAGGAA TGGCAGATGA ACCTGCTGTA 
GACACAGAAA ATGGTGAAAC TAAACCTTGT ACAAACCAGA GTGATTCTAG TAAAACTGAT 
ATTGTTTGCT GCCCACCTCC TTCCAAAAAA ATGAAGTTGT TTGGTTTTAA AGAAGATCCT 
TTTGTCTTTG TTTCTGAAGA TGATCCAATT TTTGACCCAA TCCAGACGTT CTATGCACTT 
GATCCTTCCT TCCCTAAAAA GAACTTACTG ACACGAACAC AGGAGGGAAA AAAAAGGCAG 
TTGTATATGG TCTCTAAGGA GCTGCGAAAT GTACTTCTCC ATAATAGTGA GAAGATGAAG 
GTAATTAACA CTGGTATAAA GGTGTTATGC CGCAACAATG ATGGAGAACA GTATGGTTGT 
GCTTACAGAT TAGCACAAGA GGGCATATAT ACACTTTATC CGTTCATCAA TGCGAGAATT 
GTAACTGTAT CTATCGAAGA TATCAAAGTT CTACTCACCC AAGAAAATCC TTTCCTTAGT 
AAATTTAGTA AAGAAACACA GAAACAAGCC AATAATTTTG ATATGGGAAG CATTGTCCTT 
AAATATGAAC CTGATCCACA AGAACCGGAG ACTCTGCAGT GCCCCATTGT TCTTTGTGGC 
TGGCGTGGGA AAACATCGAT CCGTTCTTTT GTACCGAAAA ACGAAAGATT ACATTATCTA 
AGAATGATGG GAGTAGAAGT GTTTAAGGAG AAAGCTGAAG TCCTTGAAAA AAAGCCTGTA 
GAAGGAAAGG CATGCGATGA GGAGCATATA GATGAGAAAA TGGACATTGA CGGTGCAAAG 
GAGGAGAGTA AAGAATTGTC GGGAAATGAG AGTGGTGATG ATGAGGACCC TAAGGAAGAA 
GACGTTATTG ACAGAGGAGT CTTGGAACAT GTAGCACTTA AAAACACCTC AGCAATCCCA 
GCATCTGTCG AAGATCAAGC TGAGGATGCC AGCGTGAGCA AAGAGTCAGT AGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820120.1
CDS	92..2488
Translation	MGRRNRRNRQRHQRSTEQRSPAEEEQRRKAREQAAWECGYPEIIKENKLFEHYYQELKIVPDGEWDKFMASLREPLPATIRITGYKSHAKEILHCLKEKYFKELQDIEVDGQKIEAPQPLSWYPEELAWHTNLSRKIIRKSPELEKFHQFLVNETESGNISRQEAVSMIPPVLLKVQPHHKILDMCAAPGSKTAQIIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLNSPCIMVVNHDASSIPRLLVENNGSREVLYYDRILCDVPCSGDGTLRKNIDVWKKWTTLNSLQLHGLQIRIATRGVEQLAEGGRMVYSTCSLNPVEDEAVIASLLDKSEGSLELADVASEIPGLKWMPGITQWKVMTKEGHWYEKWEDIPTSRHTQIRPTMFPPKDEEKLKSMNLNRCMRILPHHQNTGGFFVAVLIKKAPMPWNKRQPKLQRRPPVSACDASIAVAPELVKAVTENSAGMADEPAVDTENGETKPCTNQSDSSKTDIVCCPPPSKKMKLFGFKEDPFVFVSEDDPIFDPIQTFYALDPSFPKKNLLTRTQEGKKRQLYMVSKELRNVLLHNSEKMKVINTGIKVLCRNNDGEQYGCAYRLAQEGIYTLYPFINARIVTVSIEDIKVLLTQENPFLSKFSKETQKQANNFDMGSIVLKYEPDPQEPETLQCPIVLCGWRGKTSIRSFVPKNERLHYLRMMGVEVFKEKAEVLEKKPVEGKACDEEHIDEKMDIDGAKEESKELSGNESGDDEDPKEEDVIDRGVLEHVALKNTSAIPASVEDQAEDASVSKESVD

Target ORF information:

RefSeq Version	XM_012964666.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NOP2/Sun RNA methyltransferase family member 2 (nsun2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964666.2

ORF Insert Sequence:

ATGGGCCGCA GAAATCGGCG AAACCGCCAG AGGCACCAAA GGAGCACTGA GCAGCGAAGT 
CCCGCAGAGG AAGAGCAGCG GCGCAAAGCT AGAGAGCAGG CGGCTTGGGA ATGTGGTTAC 
CCTGAAATCA TCAAAGAAAA CAAATTATTT GAACATTATT ATCAGGAACT AAAGATTGTA 
CCTGATGGGG AGTGGGACAA GTTCATGGCT TCTCTCCGGG AACCCCTTCC GGCTACCATA 
AGAATCACAG GATACAAAAG CCATGCTAAA GAAATACTGC ATTGTCTGAA GGAGAAATAC 
TTCAAAGAAC TGCAAGATAT TGAGGTTGAT GGGCAGAAGA TAGAAGCTCC ACAGCCATTA 
AGCTGGTACC CTGAAGAGCT TGCCTGGCAT ACCAATCTGA GCAGGAAAAT AATTAGAAAG 
TCTCCAGAAC TGGAAAAGTT TCATCAGTTT CTTGTCAACG AAACAGAATC GGGCAACATT 
AGTCGGCAAG AGGCTGTTAG TATGATTCCA CCAGTCCTTC TGAAAGTTCA GCCGCATCAC 
AAGATTCTGG ATATGTGTGC TGCTCCTGGA TCAAAAACAG CCCAGATAAT TGAAATGTTA 
CATGCCGATA TGAATGTACC CTTTCCTGAG GGTTTTGTCA TTGCAAATGA TGTTGACAAT 
AAACGCTGTT ACTTACTGGT TCATCAAGCC AAGAGACTAA ACAGTCCTTG CATTATGGTA 
GTAAACCATG ATGCTTCCAG CATTCCCAGA CTACTAGTAG AGAACAATGG TTCTAGAGAG 
GTGTTGTACT ACGACAGAAT ACTCTGTGAT GTACCATGCA GCGGTGATGG GACTTTGAGG 
AAAAACATTG ACGTGTGGAA GAAGTGGACA ACTTTGAACA GCTTGCAGTT ACATGGGCTA 
CAGATTAGGA TTGCTACCCG TGGGGTAGAA CAGTTAGCAG AAGGGGGCCG AATGGTCTAT 
TCTACGTGCT CTTTGAATCC TGTTGAAGAT GAGGCTGTTA TAGCTTCTCT GTTGGACAAA 
AGTGAAGGTT CCCTGGAGCT TGCTGACGTG GCTTCAGAAA TTCCTGGCTT AAAATGGATG 
CCTGGGATTA CCCAGTGGAA GGTAATGACA AAAGAAGGAC ACTGGTATGA GAAATGGGAA 
GATATTCCCA CAAGCAGACA TACTCAGATT CGCCCAACAA TGTTTCCGCC AAAAGATGAG 
GAGAAATTGA AATCAATGAA TCTAAACCGT TGCATGCGAA TACTACCGCA TCATCAAAAC 
ACAGGAGGAT TCTTTGTAGC TGTGTTGATA AAGAAAGCAC CAATGCCATG GAACAAACGA 
CAGCCAAAGC TGCAAAGGAG ACCTCCTGTT TCAGCTTGTG ATGCATCTAT TGCTGTTGCC 
CCCGAGCTTG TGAAAGCTGT CACTGAGAAT AGTGCAGGAA TGGCAGATGA ACCTGCTGTA 
GACACAGAAA ATGGTGAAAC TAAACCTTGT ACAAACCAGA GTGATTCTAG TAAAACTGAT 
ATTGTTTGCT GCCCACCTCC TTCCAAAAAA ATGAAGTTGT TTGGTTTTAA AGAAGATCCT 
TTTGTCTTTG TTTCTGAAGA TGATCCAATT TTTGACCCAA TCCAGACGTT CTATGCACTT 
GATCCTTCCT TCCCTAAAAA GAACTTACTG ACACGAACAC AGGAGGGAAA AAAAAGGCAG 
TTGTATATGG TCTCTAAGGA GCTGCGAAAT GTACTTCTCC ATAATAGTGA GAAGATGAAG 
GTAATTAACA CTGGTATAAA GGTGTTATGC CGCAACAATG ATGGAGAACA GTATGGTTGT 
GCTTACAGAT TAGCACAAGA GGGCATATAT ACACTTTATC CGTTCATCAA TGCGAGAATT 
GTAACTGTAT CTATCGAAGA TATCAAAGTT CTACTCACCC AAGAAAATCC TTTCCTTAGT 
AAATTTAGTA AAGAAACACA GAAACAAGCC AATAATTTTG ATATGGGAAG CATTGTCCTT 
AAATATGAAC CTGATCCACA AGAACCGGAG ACTCTGCAGT GCCCCATTGT TCTTTGTGGC 
TGGCGTGGGA AAACATCGAT CCGTTCTTTT GTACCGAAAA ACGAAAGATT ACATTATCTA 
AGAATGATGG GAGTAGAAGT GTTTAAGGAG AAAGCTGAAG TCCTTGAAAA AAAGCCTGTA 
GAAGGAAAGG CATGCGATGA GGAGCATATA GATGAGAAAA TGGACATTGA CGGTGCAAAG 
GAGGAGAGTA AAGAATTGTC GGGAAATGAG AGTGGTGATG ATGAGGACCC TAAGGAAGAA 
GACGTTATTG ACAGAGGAGT CTTGGAACAT GTAGCACTTA AAAACACCTC AGCAATCCCA 
GCATCTGTCG AAGATCAAGC TGAGGATGCC AGCGTGAGCA AAGAGTCAGT AGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsun2 ( XM_012964666.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820120.1 Xenopus tropicalis NOP2/Sun RNA methyltransferase 2 (nsun2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa25768
Clone ID Related Accession (Same CDS sequence)	XM_012964665.2 , NM_001015962.2 , XM_012964666.3 , XM_012964666.2 , XM_012964665.3
Accession Version	XM_012964666.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	RNA cytosine-C(5)-methyltransferase NSUN2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964666.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCGCA GAAATCGGCG AAACCGCCAG AGGCACCAAA GGAGCACTGA GCAGCGAAGT 
CCCGCAGAGG AAGAGCAGCG GCGCAAAGCT AGAGAGCAGG CGGCTTGGGA ATGTGGTTAC 
CCTGAAATCA TCAAAGAAAA CAAATTATTT GAACATTATT ATCAGGAACT AAAGATTGTA 
CCTGATGGGG AGTGGGACAA GTTCATGGCT TCTCTCCGGG AACCCCTTCC GGCTACCATA 
AGAATCACAG GATACAAAAG CCATGCTAAA GAAATACTGC ATTGTCTGAA GGAGAAATAC 
TTCAAAGAAC TGCAAGATAT TGAGGTTGAT GGGCAGAAGA TAGAAGCTCC ACAGCCATTA 
AGCTGGTACC CTGAAGAGCT TGCCTGGCAT ACCAATCTGA GCAGGAAAAT AATTAGAAAG 
TCTCCAGAAC TGGAAAAGTT TCATCAGTTT CTTGTCAACG AAACAGAATC GGGCAACATT 
AGTCGGCAAG AGGCTGTTAG TATGATTCCA CCAGTCCTTC TGAAAGTTCA GCCGCATCAC 
AAGATTCTGG ATATGTGTGC TGCTCCTGGA TCAAAAACAG CCCAGATAAT TGAAATGTTA 
CATGCCGATA TGAATGTACC CTTTCCTGAG GGTTTTGTCA TTGCAAATGA TGTTGACAAT 
AAACGCTGTT ACTTACTGGT TCATCAAGCC AAGAGACTAA ACAGTCCTTG CATTATGGTA 
GTAAACCATG ATGCTTCCAG CATTCCCAGA CTACTAGTAG AGAACAATGG TTCTAGAGAG 
GTGTTGTACT ACGACAGAAT ACTCTGTGAT GTACCATGCA GCGGTGATGG GACTTTGAGG 
AAAAACATTG ACGTGTGGAA GAAGTGGACA ACTTTGAACA GCTTGCAGTT ACATGGGCTA 
CAGATTAGGA TTGCTACCCG TGGGGTAGAA CAGTTAGCAG AAGGGGGCCG AATGGTCTAT 
TCTACGTGCT CTTTGAATCC TGTTGAAGAT GAGGCTGTTA TAGCTTCTCT GTTGGACAAA 
AGTGAAGGTT CCCTGGAGCT TGCTGACGTG GCTTCAGAAA TTCCTGGCTT AAAATGGATG 
CCTGGGATTA CCCAGTGGAA GGTAATGACA AAAGAAGGAC ACTGGTATGA GAAATGGGAA 
GATATTCCCA CAAGCAGACA TACTCAGATT CGCCCAACAA TGTTTCCGCC AAAAGATGAG 
GAGAAATTGA AATCAATGAA TCTAAACCGT TGCATGCGAA TACTACCGCA TCATCAAAAC 
ACAGGAGGAT TCTTTGTAGC TGTGTTGATA AAGAAAGCAC CAATGCCATG GAACAAACGA 
CAGCCAAAGC TGCAAAGGAG ACCTCCTGTT TCAGCTTGTG ATGCATCTAT TGCTGTTGCC 
CCCGAGCTTG TGAAAGCTGT CACTGAGAAT AGTGCAGGAA TGGCAGATGA ACCTGCTGTA 
GACACAGAAA ATGGTGAAAC TAAACCTTGT ACAAACCAGA GTGATTCTAG TAAAACTGAT 
ATTGTTTGCT GCCCACCTCC TTCCAAAAAA ATGAAGTTGT TTGGTTTTAA AGAAGATCCT 
TTTGTCTTTG TTTCTGAAGA TGATCCAATT TTTGACCCAA TCCAGACGTT CTATGCACTT 
GATCCTTCCT TCCCTAAAAA GAACTTACTG ACACGAACAC AGGAGGGAAA AAAAAGGCAG 
TTGTATATGG TCTCTAAGGA GCTGCGAAAT GTACTTCTCC ATAATAGTGA GAAGATGAAG 
GTAATTAACA CTGGTATAAA GGTGTTATGC CGCAACAATG ATGGAGAACA GTATGGTTGT 
GCTTACAGAT TAGCACAAGA GGGCATATAT ACACTTTATC CGTTCATCAA TGCGAGAATT 
GTAACTGTAT CTATCGAAGA TATCAAAGTT CTACTCACCC AAGAAAATCC TTTCCTTAGT 
AAATTTAGTA AAGAAACACA GAAACAAGCC AATAATTTTG ATATGGGAAG CATTGTCCTT 
AAATATGAAC CTGATCCACA AGAACCGGAG ACTCTGCAGT GCCCCATTGT TCTTTGTGGC 
TGGCGTGGGA AAACATCGAT CCGTTCTTTT GTACCGAAAA ACGAAAGATT ACATTATCTA 
AGAATGATGG GAGTAGAAGT GTTTAAGGAG AAAGCTGAAG TCCTTGAAAA AAAGCCTGTA 
GAAGGAAAGG CATGCGATGA GGAGCATATA GATGAGAAAA TGGACATTGA CGGTGCAAAG 
GAGGAGAGTA AAGAATTGTC GGGAAATGAG AGTGGTGATG ATGAGGACCC TAAGGAAGAA 
GACGTTATTG ACAGAGGAGT CTTGGAACAT GTAGCACTTA AAAACACCTC AGCAATCCCA 
GCATCTGTCG AAGATCAAGC TGAGGATGCC AGCGTGAGCA AAGAGTCAGT AGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820120.1
CDS	92..2488
Translation	MGRRNRRNRQRHQRSTEQRSPAEEEQRRKAREQAAWECGYPEIIKENKLFEHYYQELKIVPDGEWDKFMASLREPLPATIRITGYKSHAKEILHCLKEKYFKELQDIEVDGQKIEAPQPLSWYPEELAWHTNLSRKIIRKSPELEKFHQFLVNETESGNISRQEAVSMIPPVLLKVQPHHKILDMCAAPGSKTAQIIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLNSPCIMVVNHDASSIPRLLVENNGSREVLYYDRILCDVPCSGDGTLRKNIDVWKKWTTLNSLQLHGLQIRIATRGVEQLAEGGRMVYSTCSLNPVEDEAVIASLLDKSEGSLELADVASEIPGLKWMPGITQWKVMTKEGHWYEKWEDIPTSRHTQIRPTMFPPKDEEKLKSMNLNRCMRILPHHQNTGGFFVAVLIKKAPMPWNKRQPKLQRRPPVSACDASIAVAPELVKAVTENSAGMADEPAVDTENGETKPCTNQSDSSKTDIVCCPPPSKKMKLFGFKEDPFVFVSEDDPIFDPIQTFYALDPSFPKKNLLTRTQEGKKRQLYMVSKELRNVLLHNSEKMKVINTGIKVLCRNNDGEQYGCAYRLAQEGIYTLYPFINARIVTVSIEDIKVLLTQENPFLSKFSKETQKQANNFDMGSIVLKYEPDPQEPETLQCPIVLCGWRGKTSIRSFVPKNERLHYLRMMGVEVFKEKAEVLEKKPVEGKACDEEHIDEKMDIDGAKEESKELSGNESGDDEDPKEEDVIDRGVLEHVALKNTSAIPASVEDQAEDASVSKESVD

Target ORF information:

RefSeq Version	XM_012964666.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NOP2/Sun RNA methyltransferase 2 (nsun2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964666.3

ORF Insert Sequence:

ATGGGCCGCA GAAATCGGCG AAACCGCCAG AGGCACCAAA GGAGCACTGA GCAGCGAAGT 
CCCGCAGAGG AAGAGCAGCG GCGCAAAGCT AGAGAGCAGG CGGCTTGGGA ATGTGGTTAC 
CCTGAAATCA TCAAAGAAAA CAAATTATTT GAACATTATT ATCAGGAACT AAAGATTGTA 
CCTGATGGGG AGTGGGACAA GTTCATGGCT TCTCTCCGGG AACCCCTTCC GGCTACCATA 
AGAATCACAG GATACAAAAG CCATGCTAAA GAAATACTGC ATTGTCTGAA GGAGAAATAC 
TTCAAAGAAC TGCAAGATAT TGAGGTTGAT GGGCAGAAGA TAGAAGCTCC ACAGCCATTA 
AGCTGGTACC CTGAAGAGCT TGCCTGGCAT ACCAATCTGA GCAGGAAAAT AATTAGAAAG 
TCTCCAGAAC TGGAAAAGTT TCATCAGTTT CTTGTCAACG AAACAGAATC GGGCAACATT 
AGTCGGCAAG AGGCTGTTAG TATGATTCCA CCAGTCCTTC TGAAAGTTCA GCCGCATCAC 
AAGATTCTGG ATATGTGTGC TGCTCCTGGA TCAAAAACAG CCCAGATAAT TGAAATGTTA 
CATGCCGATA TGAATGTACC CTTTCCTGAG GGTTTTGTCA TTGCAAATGA TGTTGACAAT 
AAACGCTGTT ACTTACTGGT TCATCAAGCC AAGAGACTAA ACAGTCCTTG CATTATGGTA 
GTAAACCATG ATGCTTCCAG CATTCCCAGA CTACTAGTAG AGAACAATGG TTCTAGAGAG 
GTGTTGTACT ACGACAGAAT ACTCTGTGAT GTACCATGCA GCGGTGATGG GACTTTGAGG 
AAAAACATTG ACGTGTGGAA GAAGTGGACA ACTTTGAACA GCTTGCAGTT ACATGGGCTA 
CAGATTAGGA TTGCTACCCG TGGGGTAGAA CAGTTAGCAG AAGGGGGCCG AATGGTCTAT 
TCTACGTGCT CTTTGAATCC TGTTGAAGAT GAGGCTGTTA TAGCTTCTCT GTTGGACAAA 
AGTGAAGGTT CCCTGGAGCT TGCTGACGTG GCTTCAGAAA TTCCTGGCTT AAAATGGATG 
CCTGGGATTA CCCAGTGGAA GGTAATGACA AAAGAAGGAC ACTGGTATGA GAAATGGGAA 
GATATTCCCA CAAGCAGACA TACTCAGATT CGCCCAACAA TGTTTCCGCC AAAAGATGAG 
GAGAAATTGA AATCAATGAA TCTAAACCGT TGCATGCGAA TACTACCGCA TCATCAAAAC 
ACAGGAGGAT TCTTTGTAGC TGTGTTGATA AAGAAAGCAC CAATGCCATG GAACAAACGA 
CAGCCAAAGC TGCAAAGGAG ACCTCCTGTT TCAGCTTGTG ATGCATCTAT TGCTGTTGCC 
CCCGAGCTTG TGAAAGCTGT CACTGAGAAT AGTGCAGGAA TGGCAGATGA ACCTGCTGTA 
GACACAGAAA ATGGTGAAAC TAAACCTTGT ACAAACCAGA GTGATTCTAG TAAAACTGAT 
ATTGTTTGCT GCCCACCTCC TTCCAAAAAA ATGAAGTTGT TTGGTTTTAA AGAAGATCCT 
TTTGTCTTTG TTTCTGAAGA TGATCCAATT TTTGACCCAA TCCAGACGTT CTATGCACTT 
GATCCTTCCT TCCCTAAAAA GAACTTACTG ACACGAACAC AGGAGGGAAA AAAAAGGCAG 
TTGTATATGG TCTCTAAGGA GCTGCGAAAT GTACTTCTCC ATAATAGTGA GAAGATGAAG 
GTAATTAACA CTGGTATAAA GGTGTTATGC CGCAACAATG ATGGAGAACA GTATGGTTGT 
GCTTACAGAT TAGCACAAGA GGGCATATAT ACACTTTATC CGTTCATCAA TGCGAGAATT 
GTAACTGTAT CTATCGAAGA TATCAAAGTT CTACTCACCC AAGAAAATCC TTTCCTTAGT 
AAATTTAGTA AAGAAACACA GAAACAAGCC AATAATTTTG ATATGGGAAG CATTGTCCTT 
AAATATGAAC CTGATCCACA AGAACCGGAG ACTCTGCAGT GCCCCATTGT TCTTTGTGGC 
TGGCGTGGGA AAACATCGAT CCGTTCTTTT GTACCGAAAA ACGAAAGATT ACATTATCTA 
AGAATGATGG GAGTAGAAGT GTTTAAGGAG AAAGCTGAAG TCCTTGAAAA AAAGCCTGTA 
GAAGGAAAGG CATGCGATGA GGAGCATATA GATGAGAAAA TGGACATTGA CGGTGCAAAG 
GAGGAGAGTA AAGAATTGTC GGGAAATGAG AGTGGTGATG ATGAGGACCC TAAGGAAGAA 
GACGTTATTG ACAGAGGAGT CTTGGAACAT GTAGCACTTA AAAACACCTC AGCAATCCCA 
GCATCTGTCG AAGATCAAGC TGAGGATGCC AGCGTGAGCA AAGAGTCAGT AGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsun2 ( XM_012964665.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820119.1 Xenopus tropicalis NOP2/Sun RNA methyltransferase 2 (nsun2), transcript variant X1, mRNA.

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CloneID	OXa25768
Clone ID Related Accession (Same CDS sequence)	XM_012964665.2 , NM_001015962.2 , XM_012964666.3 , XM_012964666.2 , XM_012964665.3
Accession Version	XM_012964665.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	RNA cytosine-C(5)-methyltransferase NSUN2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964665.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCGCA GAAATCGGCG AAACCGCCAG AGGCACCAAA GGAGCACTGA GCAGCGAAGT 
CCCGCAGAGG AAGAGCAGCG GCGCAAAGCT AGAGAGCAGG CGGCTTGGGA ATGTGGTTAC 
CCTGAAATCA TCAAAGAAAA CAAATTATTT GAACATTATT ATCAGGAACT AAAGATTGTA 
CCTGATGGGG AGTGGGACAA GTTCATGGCT TCTCTCCGGG AACCCCTTCC GGCTACCATA 
AGAATCACAG GATACAAAAG CCATGCTAAA GAAATACTGC ATTGTCTGAA GGAGAAATAC 
TTCAAAGAAC TGCAAGATAT TGAGGTTGAT GGGCAGAAGA TAGAAGCTCC ACAGCCATTA 
AGCTGGTACC CTGAAGAGCT TGCCTGGCAT ACCAATCTGA GCAGGAAAAT AATTAGAAAG 
TCTCCAGAAC TGGAAAAGTT TCATCAGTTT CTTGTCAACG AAACAGAATC GGGCAACATT 
AGTCGGCAAG AGGCTGTTAG TATGATTCCA CCAGTCCTTC TGAAAGTTCA GCCGCATCAC 
AAGATTCTGG ATATGTGTGC TGCTCCTGGA TCAAAAACAG CCCAGATAAT TGAAATGTTA 
CATGCCGATA TGAATGTACC CTTTCCTGAG GGTTTTGTCA TTGCAAATGA TGTTGACAAT 
AAACGCTGTT ACTTACTGGT TCATCAAGCC AAGAGACTAA ACAGTCCTTG CATTATGGTA 
GTAAACCATG ATGCTTCCAG CATTCCCAGA CTACTAGTAG AGAACAATGG TTCTAGAGAG 
GTGTTGTACT ACGACAGAAT ACTCTGTGAT GTACCATGCA GCGGTGATGG GACTTTGAGG 
AAAAACATTG ACGTGTGGAA GAAGTGGACA ACTTTGAACA GCTTGCAGTT ACATGGGCTA 
CAGATTAGGA TTGCTACCCG TGGGGTAGAA CAGTTAGCAG AAGGGGGCCG AATGGTCTAT 
TCTACGTGCT CTTTGAATCC TGTTGAAGAT GAGGCTGTTA TAGCTTCTCT GTTGGACAAA 
AGTGAAGGTT CCCTGGAGCT TGCTGACGTG GCTTCAGAAA TTCCTGGCTT AAAATGGATG 
CCTGGGATTA CCCAGTGGAA GGTAATGACA AAAGAAGGAC ACTGGTATGA GAAATGGGAA 
GATATTCCCA CAAGCAGACA TACTCAGATT CGCCCAACAA TGTTTCCGCC AAAAGATGAG 
GAGAAATTGA AATCAATGAA TCTAAACCGT TGCATGCGAA TACTACCGCA TCATCAAAAC 
ACAGGAGGAT TCTTTGTAGC TGTGTTGATA AAGAAAGCAC CAATGCCATG GAACAAACGA 
CAGCCAAAGC TGCAAAGGAG ACCTCCTGTT TCAGCTTGTG ATGCATCTAT TGCTGTTGCC 
CCCGAGCTTG TGAAAGCTGT CACTGAGAAT AGTGCAGGAA TGGCAGATGA ACCTGCTGTA 
GACACAGAAA ATGGTGAAAC TAAACCTTGT ACAAACCAGA GTGATTCTAG TAAAACTGAT 
ATTGTTTGCT GCCCACCTCC TTCCAAAAAA ATGAAGTTGT TTGGTTTTAA AGAAGATCCT 
TTTGTCTTTG TTTCTGAAGA TGATCCAATT TTTGACCCAA TCCAGACGTT CTATGCACTT 
GATCCTTCCT TCCCTAAAAA GAACTTACTG ACACGAACAC AGGAGGGAAA AAAAAGGCAG 
TTGTATATGG TCTCTAAGGA GCTGCGAAAT GTACTTCTCC ATAATAGTGA GAAGATGAAG 
GTAATTAACA CTGGTATAAA GGTGTTATGC CGCAACAATG ATGGAGAACA GTATGGTTGT 
GCTTACAGAT TAGCACAAGA GGGCATATAT ACACTTTATC CGTTCATCAA TGCGAGAATT 
GTAACTGTAT CTATCGAAGA TATCAAAGTT CTACTCACCC AAGAAAATCC TTTCCTTAGT 
AAATTTAGTA AAGAAACACA GAAACAAGCC AATAATTTTG ATATGGGAAG CATTGTCCTT 
AAATATGAAC CTGATCCACA AGAACCGGAG ACTCTGCAGT GCCCCATTGT TCTTTGTGGC 
TGGCGTGGGA AAACATCGAT CCGTTCTTTT GTACCGAAAA ACGAAAGATT ACATTATCTA 
AGAATGATGG GAGTAGAAGT GTTTAAGGAG AAAGCTGAAG TCCTTGAAAA AAAGCCTGTA 
GAAGGAAAGG CATGCGATGA GGAGCATATA GATGAGAAAA TGGACATTGA CGGTGCAAAG 
GAGGAGAGTA AAGAATTGTC GGGAAATGAG AGTGGTGATG ATGAGGACCC TAAGGAAGAA 
GACGTTATTG ACAGAGGAGT CTTGGAACAT GTAGCACTTA AAAACACCTC AGCAATCCCA 
GCATCTGTCG AAGATCAAGC TGAGGATGCC AGCGTGAGCA AAGAGTCAGT AGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820119.1
CDS	229..2625
Translation	MGRRNRRNRQRHQRSTEQRSPAEEEQRRKAREQAAWECGYPEIIKENKLFEHYYQELKIVPDGEWDKFMASLREPLPATIRITGYKSHAKEILHCLKEKYFKELQDIEVDGQKIEAPQPLSWYPEELAWHTNLSRKIIRKSPELEKFHQFLVNETESGNISRQEAVSMIPPVLLKVQPHHKILDMCAAPGSKTAQIIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLNSPCIMVVNHDASSIPRLLVENNGSREVLYYDRILCDVPCSGDGTLRKNIDVWKKWTTLNSLQLHGLQIRIATRGVEQLAEGGRMVYSTCSLNPVEDEAVIASLLDKSEGSLELADVASEIPGLKWMPGITQWKVMTKEGHWYEKWEDIPTSRHTQIRPTMFPPKDEEKLKSMNLNRCMRILPHHQNTGGFFVAVLIKKAPMPWNKRQPKLQRRPPVSACDASIAVAPELVKAVTENSAGMADEPAVDTENGETKPCTNQSDSSKTDIVCCPPPSKKMKLFGFKEDPFVFVSEDDPIFDPIQTFYALDPSFPKKNLLTRTQEGKKRQLYMVSKELRNVLLHNSEKMKVINTGIKVLCRNNDGEQYGCAYRLAQEGIYTLYPFINARIVTVSIEDIKVLLTQENPFLSKFSKETQKQANNFDMGSIVLKYEPDPQEPETLQCPIVLCGWRGKTSIRSFVPKNERLHYLRMMGVEVFKEKAEVLEKKPVEGKACDEEHIDEKMDIDGAKEESKELSGNESGDDEDPKEEDVIDRGVLEHVALKNTSAIPASVEDQAEDASVSKESVD

Target ORF information:

RefSeq Version	XM_012964665.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NOP2/Sun RNA methyltransferase 2 (nsun2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964665.3

ORF Insert Sequence:

ATGGGCCGCA GAAATCGGCG AAACCGCCAG AGGCACCAAA GGAGCACTGA GCAGCGAAGT 
CCCGCAGAGG AAGAGCAGCG GCGCAAAGCT AGAGAGCAGG CGGCTTGGGA ATGTGGTTAC 
CCTGAAATCA TCAAAGAAAA CAAATTATTT GAACATTATT ATCAGGAACT AAAGATTGTA 
CCTGATGGGG AGTGGGACAA GTTCATGGCT TCTCTCCGGG AACCCCTTCC GGCTACCATA 
AGAATCACAG GATACAAAAG CCATGCTAAA GAAATACTGC ATTGTCTGAA GGAGAAATAC 
TTCAAAGAAC TGCAAGATAT TGAGGTTGAT GGGCAGAAGA TAGAAGCTCC ACAGCCATTA 
AGCTGGTACC CTGAAGAGCT TGCCTGGCAT ACCAATCTGA GCAGGAAAAT AATTAGAAAG 
TCTCCAGAAC TGGAAAAGTT TCATCAGTTT CTTGTCAACG AAACAGAATC GGGCAACATT 
AGTCGGCAAG AGGCTGTTAG TATGATTCCA CCAGTCCTTC TGAAAGTTCA GCCGCATCAC 
AAGATTCTGG ATATGTGTGC TGCTCCTGGA TCAAAAACAG CCCAGATAAT TGAAATGTTA 
CATGCCGATA TGAATGTACC CTTTCCTGAG GGTTTTGTCA TTGCAAATGA TGTTGACAAT 
AAACGCTGTT ACTTACTGGT TCATCAAGCC AAGAGACTAA ACAGTCCTTG CATTATGGTA 
GTAAACCATG ATGCTTCCAG CATTCCCAGA CTACTAGTAG AGAACAATGG TTCTAGAGAG 
GTGTTGTACT ACGACAGAAT ACTCTGTGAT GTACCATGCA GCGGTGATGG GACTTTGAGG 
AAAAACATTG ACGTGTGGAA GAAGTGGACA ACTTTGAACA GCTTGCAGTT ACATGGGCTA 
CAGATTAGGA TTGCTACCCG TGGGGTAGAA CAGTTAGCAG AAGGGGGCCG AATGGTCTAT 
TCTACGTGCT CTTTGAATCC TGTTGAAGAT GAGGCTGTTA TAGCTTCTCT GTTGGACAAA 
AGTGAAGGTT CCCTGGAGCT TGCTGACGTG GCTTCAGAAA TTCCTGGCTT AAAATGGATG 
CCTGGGATTA CCCAGTGGAA GGTAATGACA AAAGAAGGAC ACTGGTATGA GAAATGGGAA 
GATATTCCCA CAAGCAGACA TACTCAGATT CGCCCAACAA TGTTTCCGCC AAAAGATGAG 
GAGAAATTGA AATCAATGAA TCTAAACCGT TGCATGCGAA TACTACCGCA TCATCAAAAC 
ACAGGAGGAT TCTTTGTAGC TGTGTTGATA AAGAAAGCAC CAATGCCATG GAACAAACGA 
CAGCCAAAGC TGCAAAGGAG ACCTCCTGTT TCAGCTTGTG ATGCATCTAT TGCTGTTGCC 
CCCGAGCTTG TGAAAGCTGT CACTGAGAAT AGTGCAGGAA TGGCAGATGA ACCTGCTGTA 
GACACAGAAA ATGGTGAAAC TAAACCTTGT ACAAACCAGA GTGATTCTAG TAAAACTGAT 
ATTGTTTGCT GCCCACCTCC TTCCAAAAAA ATGAAGTTGT TTGGTTTTAA AGAAGATCCT 
TTTGTCTTTG TTTCTGAAGA TGATCCAATT TTTGACCCAA TCCAGACGTT CTATGCACTT 
GATCCTTCCT TCCCTAAAAA GAACTTACTG ACACGAACAC AGGAGGGAAA AAAAAGGCAG 
TTGTATATGG TCTCTAAGGA GCTGCGAAAT GTACTTCTCC ATAATAGTGA GAAGATGAAG 
GTAATTAACA CTGGTATAAA GGTGTTATGC CGCAACAATG ATGGAGAACA GTATGGTTGT 
GCTTACAGAT TAGCACAAGA GGGCATATAT ACACTTTATC CGTTCATCAA TGCGAGAATT 
GTAACTGTAT CTATCGAAGA TATCAAAGTT CTACTCACCC AAGAAAATCC TTTCCTTAGT 
AAATTTAGTA AAGAAACACA GAAACAAGCC AATAATTTTG ATATGGGAAG CATTGTCCTT 
AAATATGAAC CTGATCCACA AGAACCGGAG ACTCTGCAGT GCCCCATTGT TCTTTGTGGC 
TGGCGTGGGA AAACATCGAT CCGTTCTTTT GTACCGAAAA ACGAAAGATT ACATTATCTA 
AGAATGATGG GAGTAGAAGT GTTTAAGGAG AAAGCTGAAG TCCTTGAAAA AAAGCCTGTA 
GAAGGAAAGG CATGCGATGA GGAGCATATA GATGAGAAAA TGGACATTGA CGGTGCAAAG 
GAGGAGAGTA AAGAATTGTC GGGAAATGAG AGTGGTGATG ATGAGGACCC TAAGGAAGAA 
GACGTTATTG ACAGAGGAGT CTTGGAACAT GTAGCACTTA AAAACACCTC AGCAATCCCA 
GCATCTGTCG AAGATCAAGC TGAGGATGCC AGCGTGAGCA AAGAGTCAGT AGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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nsun2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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