zeb1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	zeb1
Entrez Gene ID	548525
Full Name	zinc finger E-box binding homeobox 1
Synonyms	areb6,bzp,deltaef1,fecd6,nil-2-a,nil-2a,nil2a,tcf8,zeb,zfhep,zfhx1,zfhx1a
General protein information	Preferred Names zinc finger E-box binding homeobox 1 Names zinc finger E-box-binding homeobox 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012964650.2	XP_012820104.1	zinc finger E-box-binding homeobox 1 isoform X1
XM_012964652.2	XP_012820106.1	zinc finger E-box-binding homeobox 1 isoform X2
NM_001015808.1	NP_001015808.1	zinc finger E-box-binding homeobox 1
XM_012964653.3	XP_012820107.1	zinc finger E-box-binding homeobox 1 isoform X3
XM_012964653.2	XP_012820107.1	zinc finger E-box-binding homeobox 1 isoform X3
XM_012964650.3	XP_012820104.1	zinc finger E-box-binding homeobox 1 isoform X1
XM_031903367.1	XP_031759227.1	zinc finger E-box-binding homeobox 1 isoform X2
XM_031903368.1	XP_031759228.1	zinc finger E-box-binding homeobox 1 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	ZEB1	NP_001167567.1
Bos taurus (cattle)	ZEB1	NP_001193519.1
Mus musculus (house mouse)	Zeb1	NP_035676.1
Gallus gallus (chicken)	ZEB1	NP_990462.1
Canis lupus familiaris (dog)	ZEB1	XP_003638927.1
	ZEB1	XP_001089463.2
Xenopus tropicalis (tropical clawed frog)	zeb1	NP_001015808.1
Pan troglodytes (chimpanzee)	ZEB1	XP_003312560.1
Rattus norvegicus (Norway rat)	Zeb1	NP_037296.1
Danio rerio (zebrafish)	zeb1b	NP_571784.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following zeb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the zeb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa27418	XM_012964652.2 Latest version!	Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa27419	XM_031903368.1 Latest version!	Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa27419	XM_012964653.3 Latest version!	Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa59678	XM_012964650.3 Latest version!	Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa59679	XM_031903367.1 Latest version!	Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa24476	NM_001015808.1 Latest version!	Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OXa27416	XM_012964650.2 Latest version!	Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
OXa27419	XM_012964653.2	Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

zeb1 ( XM_012964652.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820106.1 Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa27418
Clone ID Related Accession (Same CDS sequence)	XM_012964652.2
Accession Version	XM_012964652.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger E-box-binding homeobox 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964652.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG GCCCCAGGTG TAAGCGCAGA AAACAGGCGA ACCCCAGACG GAATAACGTT 
ACCAATTACA ACAGTGTGCT CGAAGCAAAT TCTGATTCGG ATGATGAAGA CAAATTGCAC 
ATAGTGGAAG AGGAAAGTGT TACAGACGCA GCAGATGGCG AGGGCAGCGT TCCAGAAGAT 
GACTTGCCAA CAGACCAAAC AGTATTACCA GAAGGCAGCG AGAGCGCAAA GGGGAGCGGC 
AAAACTTGCT GGGAAGATGA AGAAGGGGCA GATTGCAGTG ATGAGATCCT GGGGCCTGAA 
GCCGAAGCTG ATGAACTTGG ATGTACAGTT AAAGATGAAG AATGCGATTC AGACCTAGAA 
AATGAACAAA ACCAAGATCC CAACGTTGAA GAATTCCTAC AGCAAAGCGA CACTGCTGTA 
ATCTATCCGG AAGCGCCAGA GGATGACCAG AGGCAAGGCA CACCGGAGGC GGTTGGTCAA 
GATGAAAATG GAACGCCAGA CGCATTCTCC CAGCTCCTCA CCTGCCCGTA TTGTGACAGG 
GGATACAAGC GCTTCACGTC TTTGAAGGAA CATATTAAGT ACCGCCATGA GAAAAATGAA 
GACAATTTCA GTTGCTCGAT GTGTAGTTAT ACCTTTGCTT ATAGAACCCA GCTTGATCGT 
CACATGACCT CTCATAAATC AGGAAAAGAT CAGAGACATG TGACGCAGTC TGGAGGCAAC 
CGTAAATTCA AATGCCCTGA GTGTGGAAAA GCTTTTAAAT ACAAACACCA TCTGAAAGAA 
CACTTACGGA TTCACAGTGG AGAGAAGCCA TACGAGTGCC CAAACTGCAA GAAGCGATTT 
TCTCATTCTG GTTCCTACAG TTCCCACATA AGCAGTAAGA AATGTATCGG TGTGGCGCCT 
GTGAATGGAC GCGTGCGCTC AGGGCTGAAG ACTCCCCAGT GCTCCTCCCC GTCCCTTTCT 
GCTTCTCCGG GAAGTCCAGC TCGACCACAG ATCCGCCAGA AGATAGAGAA CAAACCTTTG 
CAAGAGCAGA TTCCAGTTAA CCAAATCAAA ACCGAACCTG TGGATTATGA AATCAAGCCT 
ATCGTGGTGA CATCAGGAAT CAACTGCTCA GCCCCTTTGC AAAATGGGGT GTTTAGTAGT 
GGTAGCCGTT TGCAGGCAAC TAATTCTCCT CAGGGTGTTG TACAAGCTGT TGTTCTGCCA 
ACTATGGGTC TGGTGTCCCC CATAAGTATT AACCTTAATG ACATTCAGAA TGTTCTTAAG 
GTGGCAGTTG ATGGGAATGT AATAAGACAA GTATTAGAAA ATAACCATGT CAGTCTTGCA 
TCCAAAGAAC AAGGGACTCT CACCTCCGCC ACAATCCAAC AAGCCGGCCA TTCTGTTATT 
TCCGCTATTA GTCTTCCATT GGTTGATCAA GACGGTACAA CCAAGATCAT CATCAATTAC 
AGCTTAGAGC AACCTAGTCA GCTTCCCCTC GTTGCGCAAA ATTTGAAAAA AGAAGCCACA 
GCTCCTGCAG AAGTCTGCAA AACGGAGAAG TTGCCAGAAG ATCTCACTAT CAAAACTGAA 
AAGGATACTA AGTTTACAGA AGAAAGCACT TGTTTACTTG GTGCCGATCA CCCAGGAGAC 
AAAAATGCAC TTCAAGAACC CAAGCACTAT GATCAGAAAA AGAACAGCCT ACTTCCAGAG 
AGTAGCAAAG CCGAACCTAT CAAATCCGAG CCAACTTCTG CCGACCCCGA AGATGGCAAT 
CTTACTCCAA GCCAACCACC TTTAAAGAAT CTCTTATCTC TTCTGAAAGC ATACTATGCT 
TTAAATGCTC AGCCGAGCTC CGAGGAACTT TCTAAAATTG CAGATTCAGT AAACCTACCA 
CTCGACGTGG TAAAAGACTG GTTTGAAAAA ATGCAGAATG GAGAAATATC TGTGGAGTCA 
TCTCGGCCCT CATCTCCAAA ACCTGGACAA GACAGTGTTA CAGAAAACAG TGTGGAATCT 
AAAGATGAAA GTGGATTGCA TAACGGCACG AACGTTTCAC TGAAATCCCC CAAACAAGAA 
CCTGAGGAAC CGTTAGCTGT TAATGGTCCA GAAAGTGGCA CAGCTTCTCC TACACCACTA 
AACCTCTCTT CTTTAAAAAA CTCAGAAGGT GCAGCCTGCT CCGATGGAAC CCCAGATGGG 
GAGCAGCAGG CCGAGCCTCT TGATCTATCC TTACCAAAGC CACATGGAAA TGTGCCGGAG 
CGACCTTCCA TCACTAGTGT TTACCAGAGC AGTTCATATT CTGTTCAAGA AGAACCCTTG 
AACTTGTCTT GTGTTAAAAA AGAGCCAATG CAAGTGGACG ATGTTACAAA AGCAGAGCCA 
ATGCTAATTA TTAACCCAAG TGCCAATCCA ATAAATATTG CTATTCCTAC AGTCACTGCC 
CAGTTACCTA CAATCGTTGC CATTGCCGAT CAAAACGGTG TTCCGTGTTT AAGAGCTCTA 
GCTGCCAGTA AGCGAACTAT CTTGATTCCC CAAGTGACGT ACACTTACTC AACCACTGCT 
AATCCTTCAG TACCGGAACC GCAACTCAAG AACATCCAGC CTAATGGAAA TCAGGATGAG 
CGGCAGGATA CAAGCTCGGA AGGAGTGTCA AATGTAGAAG ATCAGAATGA CTCTGATTCC 
ACCCCACCCA AAAAGAAACT GAAAAAAACA GAGAACGGTC TCTATGCCTG TGACCTCTGT 
GACAAAATTT TCCAGAAAAG CAGCTCACTG CTGAGACATA AGTATGAACA CACAGGGAAT 
AACCCAGTCG ATGGGCTTTC AGTTGCCTAC AGAGGTCGCT CTGCAACTCT GTGCTTTGCC 
GGAAGTTCTA GATCGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820106.1
CDS	49..2889
Translation	MADGPRCKRRKQANPRRNNVTNYNSVLEANSDSDDEDKLHIVEEESVTDAADGEGSVPEDDLPTDQTVLPEGSESAKGSGKTCWEDEEGADCSDEILGPEAEADELGCTVKDEECDSDLENEQNQDPNVEEFLQQSDTAVIYPEAPEDDQRQGTPEAVGQDENGTPDAFSQLLTCPYCDRGYKRFTSLKEHIKYRHEKNEDNFSCSMCSYTFAYRTQLDRHMTSHKSGKDQRHVTQSGGNRKFKCPECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGVAPVNGRVRSGLKTPQCSSPSLSASPGSPARPQIRQKIENKPLQEQIPVNQIKTEPVDYEIKPIVVTSGINCSAPLQNGVFSSGSRLQATNSPQGVVQAVVLPTMGLVSPISINLNDIQNVLKVAVDGNVIRQVLENNHVSLASKEQGTLTSATIQQAGHSVISAISLPLVDQDGTTKIIINYSLEQPSQLPLVAQNLKKEATAPAEVCKTEKLPEDLTIKTEKDTKFTEESTCLLGADHPGDKNALQEPKHYDQKKNSLLPESSKAEPIKSEPTSADPEDGNLTPSQPPLKNLLSLLKAYYALNAQPSSEELSKIADSVNLPLDVVKDWFEKMQNGEISVESSRPSSPKPGQDSVTENSVESKDESGLHNGTNVSLKSPKQEPEEPLAVNGPESGTASPTPLNLSSLKNSEGAACSDGTPDGEQQAEPLDLSLPKPHGNVPERPSITSVYQSSSYSVQEEPLNLSCVKKEPMQVDDVTKAEPMLIINPSANPINIAIPTVTAQLPTIVAIADQNGVPCLRALAASKRTILIPQVTYTYSTTANPSVPEPQLKNIQPNGNQDERQDTSSEGVSNVEDQNDSDSTPPKKKLKKTENGLYACDLCDKIFQKSSSLLRHKYEHTGNNPVDGLSVAYRGRSATLCFAGSSRSS

Target ORF information:

RefSeq Version	XM_012964652.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964652.2

ORF Insert Sequence:

ATGGCGGATG GCCCCAGGTG TAAGCGCAGA AAACAGGCGA ACCCCAGACG GAATAACGTT 
ACCAATTACA ACAGTGTGCT CGAAGCAAAT TCTGATTCGG ATGATGAAGA CAAATTGCAC 
ATAGTGGAAG AGGAAAGTGT TACAGACGCA GCAGATGGCG AGGGCAGCGT TCCAGAAGAT 
GACTTGCCAA CAGACCAAAC AGTATTACCA GAAGGCAGCG AGAGCGCAAA GGGGAGCGGC 
AAAACTTGCT GGGAAGATGA AGAAGGGGCA GATTGCAGTG ATGAGATCCT GGGGCCTGAA 
GCCGAAGCTG ATGAACTTGG ATGTACAGTT AAAGATGAAG AATGCGATTC AGACCTAGAA 
AATGAACAAA ACCAAGATCC CAACGTTGAA GAATTCCTAC AGCAAAGCGA CACTGCTGTA 
ATCTATCCGG AAGCGCCAGA GGATGACCAG AGGCAAGGCA CACCGGAGGC GGTTGGTCAA 
GATGAAAATG GAACGCCAGA CGCATTCTCC CAGCTCCTCA CCTGCCCGTA TTGTGACAGG 
GGATACAAGC GCTTCACGTC TTTGAAGGAA CATATTAAGT ACCGCCATGA GAAAAATGAA 
GACAATTTCA GTTGCTCGAT GTGTAGTTAT ACCTTTGCTT ATAGAACCCA GCTTGATCGT 
CACATGACCT CTCATAAATC AGGAAAAGAT CAGAGACATG TGACGCAGTC TGGAGGCAAC 
CGTAAATTCA AATGCCCTGA GTGTGGAAAA GCTTTTAAAT ACAAACACCA TCTGAAAGAA 
CACTTACGGA TTCACAGTGG AGAGAAGCCA TACGAGTGCC CAAACTGCAA GAAGCGATTT 
TCTCATTCTG GTTCCTACAG TTCCCACATA AGCAGTAAGA AATGTATCGG TGTGGCGCCT 
GTGAATGGAC GCGTGCGCTC AGGGCTGAAG ACTCCCCAGT GCTCCTCCCC GTCCCTTTCT 
GCTTCTCCGG GAAGTCCAGC TCGACCACAG ATCCGCCAGA AGATAGAGAA CAAACCTTTG 
CAAGAGCAGA TTCCAGTTAA CCAAATCAAA ACCGAACCTG TGGATTATGA AATCAAGCCT 
ATCGTGGTGA CATCAGGAAT CAACTGCTCA GCCCCTTTGC AAAATGGGGT GTTTAGTAGT 
GGTAGCCGTT TGCAGGCAAC TAATTCTCCT CAGGGTGTTG TACAAGCTGT TGTTCTGCCA 
ACTATGGGTC TGGTGTCCCC CATAAGTATT AACCTTAATG ACATTCAGAA TGTTCTTAAG 
GTGGCAGTTG ATGGGAATGT AATAAGACAA GTATTAGAAA ATAACCATGT CAGTCTTGCA 
TCCAAAGAAC AAGGGACTCT CACCTCCGCC ACAATCCAAC AAGCCGGCCA TTCTGTTATT 
TCCGCTATTA GTCTTCCATT GGTTGATCAA GACGGTACAA CCAAGATCAT CATCAATTAC 
AGCTTAGAGC AACCTAGTCA GCTTCCCCTC GTTGCGCAAA ATTTGAAAAA AGAAGCCACA 
GCTCCTGCAG AAGTCTGCAA AACGGAGAAG TTGCCAGAAG ATCTCACTAT CAAAACTGAA 
AAGGATACTA AGTTTACAGA AGAAAGCACT TGTTTACTTG GTGCCGATCA CCCAGGAGAC 
AAAAATGCAC TTCAAGAACC CAAGCACTAT GATCAGAAAA AGAACAGCCT ACTTCCAGAG 
AGTAGCAAAG CCGAACCTAT CAAATCCGAG CCAACTTCTG CCGACCCCGA AGATGGCAAT 
CTTACTCCAA GCCAACCACC TTTAAAGAAT CTCTTATCTC TTCTGAAAGC ATACTATGCT 
TTAAATGCTC AGCCGAGCTC CGAGGAACTT TCTAAAATTG CAGATTCAGT AAACCTACCA 
CTCGACGTGG TAAAAGACTG GTTTGAAAAA ATGCAGAATG GAGAAATATC TGTGGAGTCA 
TCTCGGCCCT CATCTCCAAA ACCTGGACAA GACAGTGTTA CAGAAAACAG TGTGGAATCT 
AAAGATGAAA GTGGATTGCA TAACGGCACG AACGTTTCAC TGAAATCCCC CAAACAAGAA 
CCTGAGGAAC CGTTAGCTGT TAATGGTCCA GAAAGTGGCA CAGCTTCTCC TACACCACTA 
AACCTCTCTT CTTTAAAAAA CTCAGAAGGT GCAGCCTGCT CCGATGGAAC CCCAGATGGG 
GAGCAGCAGG CCGAGCCTCT TGATCTATCC TTACCAAAGC CACATGGAAA TGTGCCGGAG 
CGACCTTCCA TCACTAGTGT TTACCAGAGC AGTTCATATT CTGTTCAAGA AGAACCCTTG 
AACTTGTCTT GTGTTAAAAA AGAGCCAATG CAAGTGGACG ATGTTACAAA AGCAGAGCCA 
ATGCTAATTA TTAACCCAAG TGCCAATCCA ATAAATATTG CTATTCCTAC AGTCACTGCC 
CAGTTACCTA CAATCGTTGC CATTGCCGAT CAAAACGGTG TTCCGTGTTT AAGAGCTCTA 
GCTGCCAGTA AGCGAACTAT CTTGATTCCC CAAGTGACGT ACACTTACTC AACCACTGCT 
AATCCTTCAG TACCGGAACC GCAACTCAAG AACATCCAGC CTAATGGAAA TCAGGATGAG 
CGGCAGGATA CAAGCTCGGA AGGAGTGTCA AATGTAGAAG ATCAGAATGA CTCTGATTCC 
ACCCCACCCA AAAAGAAACT GAAAAAAACA GAGAACGGTC TCTATGCCTG TGACCTCTGT 
GACAAAATTT TCCAGAAAAG CAGCTCACTG CTGAGACATA AGTATGAACA CACAGGGAAT 
AACCCAGTCG ATGGGCTTTC AGTTGCCTAC AGAGGTCGCT CTGCAACTCT GTGCTTTGCC 
GGAAGTTCTA GATCGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zeb1 ( XM_012964653.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820107.1 Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa27419
Clone ID Related Accession (Same CDS sequence)	XM_012964653.3 , XM_012964653.2 , XM_031903368.1
Accession Version	XM_012964653.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger E-box-binding homeobox 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964653.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTAGTT ATACCTTTGC TTATAGAACC CAGCTTGATC GTCACATGAC CTCTCATAAA 
TCAGGAAAAG ATCAGAGACA TGTGACGCAG TCTGGAGGCA ACCGTAAATT CAAATGCCCT 
GAGTGTGGAA AAGCTTTTAA ATACAAACAC CATCTGAAAG AACACTTACG GATTCACAGT 
GGAGAGAAGC CATACGAGTG CCCAAACTGC AAGAAGCGAT TTTCTCATTC TGGTTCCTAC 
AGTTCCCACA TAAGCAGTAA GAAATGTATC GGTGTGGCGC CTGTGAATGG ACGCGTGCGC 
TCAGGGCTGA AGACTCCCCA GTGCTCCTCC CCGTCCCTTT CTGCTTCTCC GGGAAGTCCA 
GCTCGACCAC AGATCCGCCA GAAGATAGAG AACAAACCTT TGCAAGAGCA GATTCCAGTT 
AACCAAATCA AAACCGAACC TGTGGATTAT GAAATCAAGC CTATCGTGGT GACATCAGGA 
ATCAACTGCT CAGCCCCTTT GCAAAATGGG GTGTTTAGTA GTGGTAGCCG TTTGCAGGCA 
ACTAATTCTC CTCAGGGTGT TGTACAAGCT GTTGTTCTGC CAACTATGGG TCTGGTGTCC 
CCCATAAGTA TTAACCTTAA TGACATTCAG AATGTTCTTA AGGTGGCAGT TGATGGGAAT 
GTAATAAGAC AAGTATTAGA AAATAACCAT GTCAGTCTTG CATCCAAAGA ACAAGGGACT 
CTCACCTCCG CCACAATCCA ACAAGCCGGC CATTCTGTTA TTTCCGCTAT TAGTCTTCCA 
TTGGTTGATC AAGACGGTAC AACCAAGATC ATCATCAATT ACAGCTTAGA GCAACCTAGT 
CAGCTTCCCC TCGTTGCGCA AAATTTGAAA AAAGAAGCCA CAGCTCCTGC AGAAGTCTGC 
AAAACGGAGA AGTTGCCAGA AGATCTCACT ATCAAAACTG AAAAGGATAC TAAGTTTACA 
GAAGAAAGCA CTTGTTTACT TGGTGCCGAT CACCCAGGAG ACAAAAATGC ACTTCAAGAA 
CCCAAGCACT ATGATCAGAA AAAGAACAGC CTACTTCCAG AGAGTAGCAA AGCCGAACCT 
ATCAAATCCG AGCCAACTTC TGCCGACCCC GAAGATGGCA ATCTTACTCC AAGCCAACCA 
CCTTTAAAGA ATCTCTTATC TCTTCTGAAA GCATACTATG CTTTAAATGC TCAGCCGAGC 
TCCGAGGAAC TTTCTAAAAT TGCAGATTCA GTAAACCTAC CACTCGACGT GGTAAAAGAC 
TGGTTTGAAA AAATGCAGAA TGGAGAAATA TCTGTGGAGT CATCTCGGCC CTCATCTCCA 
AAACCTGGAC AAGACAGTGT TACAGAAAAC AGTGTGGAAT CTAAAGATGA AAGTGGATTG 
CATAACGGCA CGAACGTTTC ACTGAAATCC CCCAAACAAG AACCTGAGGA ACCGTTAGCT 
GTTAATGGTC CAGAAAGTGG CACAGCTTCT CCTACACCAC TAAACCTCTC TTCTTTAAAA 
AACTCAGAAG GTGCAGCCTG CTCCGATGGA ACCCCAGATG GGGAGCAGCA GGCCGAGCCT 
CTTGATCTAT CCTTACCAAA GCCACATGGA AATGTGCCGG AGCGACCTTC CATCACTAGT 
GTTTACCAGA GCAGTTCATA TTCTGTTCAA GAAGAACCCT TGAACTTGTC TTGTGTTAAA 
AAAGAGCCAA TGCAAGTGGA CGATGTTACA AAAGCAGAGC CAATGCTAAT TATTAACCCA 
AGTGCCAATC CAATAAATAT TGCTATTCCT ACAGTCACTG CCCAGTTACC TACAATCGTT 
GCCATTGCCG ATCAAAACGG TGTTCCGTGT TTAAGAGCTC TAGCTGCCAG TAAGCGAACT 
ATCTTGATTC CCCAAGTGAC GTACACTTAC TCAACCACTG CTAATCCTTC AGTACCGGAA 
CCGCAACTCA AGAACATCCA GCCTAATGGA AATCAGGATG AGCGGCAGGA TACAAGCTCG 
GAAGGAGTGT CAAATGTAGA AGATCAGAAT GACTCTGATT CCACCCCACC CAAAAAGAAA 
CTGAAAAAAA CAGAGAACGG TCTCTATGCC TGTGACCTCT GTGACAAAAT TTTCCAGAAA 
AGCAGCTCAC TGCTGAGACA TAAGTATGAA CACACAGGTA AGAGGCCACA TGAATGTGGA 
ATCTGTGCAA AGGCATTTAA ACACAAACAC CATTTAATTG AGCATATGCG CCTACATTCT 
GGAGAAAAGC CCTATCAATG TGACAAATGT GGGAAACGGT TTTCGCACTC CGGATCCTAC 
TCTCAGCACA TGAACCACCG CTACTCTTAC TGCAAGAAGG AAGCCGAGGA ACGAGATGGC 
ATGGAGCAGG AAGAATCCGC TCAGGAGATC CTTGGCAACG AGAACGTGGA TTCCCAGGTG 
TCTCCATCTC AGATAGACTC TGATGAGCGC GAGAGCATAA CCCGGGAGGA AGACAGTGAG 
AAGGAAGAGG AGGATAAGGA AGAAGACGAT AAGGAAGAGG AGGATGAGAA AGAGGAGGAT 
AAGGAAGAGG AGGAAAAGGA AGAGGAGGAT GAGAAAGAGG AGGATGAGAA AGATATGGAT 
GATGGACAGG AAGAAAAGGA ACATGTTGGA GAGGGGGAAA TGGAAGAGGG AGAAGCAGAA 
CCATTGGCAG GTACTTTGAA GGATGAAGAA TGTGTAGAGG AAGACAAATG TTTGGAAGAA 
GAGGTCACAG AAGAGAATGT ATCGGAGAAA GCCCTCCCTC AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820107.1
CDS	730..3477
Translation	MCSYTFAYRTQLDRHMTSHKSGKDQRHVTQSGGNRKFKCPECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGVAPVNGRVRSGLKTPQCSSPSLSASPGSPARPQIRQKIENKPLQEQIPVNQIKTEPVDYEIKPIVVTSGINCSAPLQNGVFSSGSRLQATNSPQGVVQAVVLPTMGLVSPISINLNDIQNVLKVAVDGNVIRQVLENNHVSLASKEQGTLTSATIQQAGHSVISAISLPLVDQDGTTKIIINYSLEQPSQLPLVAQNLKKEATAPAEVCKTEKLPEDLTIKTEKDTKFTEESTCLLGADHPGDKNALQEPKHYDQKKNSLLPESSKAEPIKSEPTSADPEDGNLTPSQPPLKNLLSLLKAYYALNAQPSSEELSKIADSVNLPLDVVKDWFEKMQNGEISVESSRPSSPKPGQDSVTENSVESKDESGLHNGTNVSLKSPKQEPEEPLAVNGPESGTASPTPLNLSSLKNSEGAACSDGTPDGEQQAEPLDLSLPKPHGNVPERPSITSVYQSSSYSVQEEPLNLSCVKKEPMQVDDVTKAEPMLIINPSANPINIAIPTVTAQLPTIVAIADQNGVPCLRALAASKRTILIPQVTYTYSTTANPSVPEPQLKNIQPNGNQDERQDTSSEGVSNVEDQNDSDSTPPKKKLKKTENGLYACDLCDKIFQKSSSLLRHKYEHTGKRPHECGICAKAFKHKHHLIEHMRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKKEAEERDGMEQEESAQEILGNENVDSQVSPSQIDSDERESITREEDSEKEEEDKEEDDKEEEDEKEEDKEEEEKEEEDEKEEDEKDMDDGQEEKEHVGEGEMEEGEAEPLAGTLKDEECVEEDKCLEEEVTEENVSEKALPQP

Target ORF information:

RefSeq Version	XM_012964653.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964653.3

ORF Insert Sequence:

ATGTGTAGTT ATACCTTTGC TTATAGAACC CAGCTTGATC GTCACATGAC CTCTCATAAA 
TCAGGAAAAG ATCAGAGACA TGTGACGCAG TCTGGAGGCA ACCGTAAATT CAAATGCCCT 
GAGTGTGGAA AAGCTTTTAA ATACAAACAC CATCTGAAAG AACACTTACG GATTCACAGT 
GGAGAGAAGC CATACGAGTG CCCAAACTGC AAGAAGCGAT TTTCTCATTC TGGTTCCTAC 
AGTTCCCACA TAAGCAGTAA GAAATGTATC GGTGTGGCGC CTGTGAATGG ACGCGTGCGC 
TCAGGGCTGA AGACTCCCCA GTGCTCCTCC CCGTCCCTTT CTGCTTCTCC GGGAAGTCCA 
GCTCGACCAC AGATCCGCCA GAAGATAGAG AACAAACCTT TGCAAGAGCA GATTCCAGTT 
AACCAAATCA AAACCGAACC TGTGGATTAT GAAATCAAGC CTATCGTGGT GACATCAGGA 
ATCAACTGCT CAGCCCCTTT GCAAAATGGG GTGTTTAGTA GTGGTAGCCG TTTGCAGGCA 
ACTAATTCTC CTCAGGGTGT TGTACAAGCT GTTGTTCTGC CAACTATGGG TCTGGTGTCC 
CCCATAAGTA TTAACCTTAA TGACATTCAG AATGTTCTTA AGGTGGCAGT TGATGGGAAT 
GTAATAAGAC AAGTATTAGA AAATAACCAT GTCAGTCTTG CATCCAAAGA ACAAGGGACT 
CTCACCTCCG CCACAATCCA ACAAGCCGGC CATTCTGTTA TTTCCGCTAT TAGTCTTCCA 
TTGGTTGATC AAGACGGTAC AACCAAGATC ATCATCAATT ACAGCTTAGA GCAACCTAGT 
CAGCTTCCCC TCGTTGCGCA AAATTTGAAA AAAGAAGCCA CAGCTCCTGC AGAAGTCTGC 
AAAACGGAGA AGTTGCCAGA AGATCTCACT ATCAAAACTG AAAAGGATAC TAAGTTTACA 
GAAGAAAGCA CTTGTTTACT TGGTGCCGAT CACCCAGGAG ACAAAAATGC ACTTCAAGAA 
CCCAAGCACT ATGATCAGAA AAAGAACAGC CTACTTCCAG AGAGTAGCAA AGCCGAACCT 
ATCAAATCCG AGCCAACTTC TGCCGACCCC GAAGATGGCA ATCTTACTCC AAGCCAACCA 
CCTTTAAAGA ATCTCTTATC TCTTCTGAAA GCATACTATG CTTTAAATGC TCAGCCGAGC 
TCCGAGGAAC TTTCTAAAAT TGCAGATTCA GTAAACCTAC CACTCGACGT GGTAAAAGAC 
TGGTTTGAAA AAATGCAGAA TGGAGAAATA TCTGTGGAGT CATCTCGGCC CTCATCTCCA 
AAACCTGGAC AAGACAGTGT TACAGAAAAC AGTGTGGAAT CTAAAGATGA AAGTGGATTG 
CATAACGGCA CGAACGTTTC ACTGAAATCC CCCAAACAAG AACCTGAGGA ACCGTTAGCT 
GTTAATGGTC CAGAAAGTGG CACAGCTTCT CCTACACCAC TAAACCTCTC TTCTTTAAAA 
AACTCAGAAG GTGCAGCCTG CTCCGATGGA ACCCCAGATG GGGAGCAGCA GGCCGAGCCT 
CTTGATCTAT CCTTACCAAA GCCACATGGA AATGTGCCGG AGCGACCTTC CATCACTAGT 
GTTTACCAGA GCAGTTCATA TTCTGTTCAA GAAGAACCCT TGAACTTGTC TTGTGTTAAA 
AAAGAGCCAA TGCAAGTGGA CGATGTTACA AAAGCAGAGC CAATGCTAAT TATTAACCCA 
AGTGCCAATC CAATAAATAT TGCTATTCCT ACAGTCACTG CCCAGTTACC TACAATCGTT 
GCCATTGCCG ATCAAAACGG TGTTCCGTGT TTAAGAGCTC TAGCTGCCAG TAAGCGAACT 
ATCTTGATTC CCCAAGTGAC GTACACTTAC TCAACCACTG CTAATCCTTC AGTACCGGAA 
CCGCAACTCA AGAACATCCA GCCTAATGGA AATCAGGATG AGCGGCAGGA TACAAGCTCG 
GAAGGAGTGT CAAATGTAGA AGATCAGAAT GACTCTGATT CCACCCCACC CAAAAAGAAA 
CTGAAAAAAA CAGAGAACGG TCTCTATGCC TGTGACCTCT GTGACAAAAT TTTCCAGAAA 
AGCAGCTCAC TGCTGAGACA TAAGTATGAA CACACAGGTA AGAGGCCACA TGAATGTGGA 
ATCTGTGCAA AGGCATTTAA ACACAAACAC CATTTAATTG AGCATATGCG CCTACATTCT 
GGAGAAAAGC CCTATCAATG TGACAAATGT GGGAAACGGT TTTCGCACTC CGGATCCTAC 
TCTCAGCACA TGAACCACCG CTACTCTTAC TGCAAGAAGG AAGCCGAGGA ACGAGATGGC 
ATGGAGCAGG AAGAATCCGC TCAGGAGATC CTTGGCAACG AGAACGTGGA TTCCCAGGTG 
TCTCCATCTC AGATAGACTC TGATGAGCGC GAGAGCATAA CCCGGGAGGA AGACAGTGAG 
AAGGAAGAGG AGGATAAGGA AGAAGACGAT AAGGAAGAGG AGGATGAGAA AGAGGAGGAT 
AAGGAAGAGG AGGAAAAGGA AGAGGAGGAT GAGAAAGAGG AGGATGAGAA AGATATGGAT 
GATGGACAGG AAGAAAAGGA ACATGTTGGA GAGGGGGAAA TGGAAGAGGG AGAAGCAGAA 
CCATTGGCAG GTACTTTGAA GGATGAAGAA TGTGTAGAGG AAGACAAATG TTTGGAAGAA 
GAGGTCACAG AAGAGAATGT ATCGGAGAAA GCCCTCCCTC AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zeb1 ( XM_031903368.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759228.1 Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa27419
Clone ID Related Accession (Same CDS sequence)	XM_012964653.3 , XM_012964653.2 , XM_031903368.1
Accession Version	XM_031903368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger E-box-binding homeobox 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTAGTT ATACCTTTGC TTATAGAACC CAGCTTGATC GTCACATGAC CTCTCATAAA 
TCAGGAAAAG ATCAGAGACA TGTGACGCAG TCTGGAGGCA ACCGTAAATT CAAATGCCCT 
GAGTGTGGAA AAGCTTTTAA ATACAAACAC CATCTGAAAG AACACTTACG GATTCACAGT 
GGAGAGAAGC CATACGAGTG CCCAAACTGC AAGAAGCGAT TTTCTCATTC TGGTTCCTAC 
AGTTCCCACA TAAGCAGTAA GAAATGTATC GGTGTGGCGC CTGTGAATGG ACGCGTGCGC 
TCAGGGCTGA AGACTCCCCA GTGCTCCTCC CCGTCCCTTT CTGCTTCTCC GGGAAGTCCA 
GCTCGACCAC AGATCCGCCA GAAGATAGAG AACAAACCTT TGCAAGAGCA GATTCCAGTT 
AACCAAATCA AAACCGAACC TGTGGATTAT GAAATCAAGC CTATCGTGGT GACATCAGGA 
ATCAACTGCT CAGCCCCTTT GCAAAATGGG GTGTTTAGTA GTGGTAGCCG TTTGCAGGCA 
ACTAATTCTC CTCAGGGTGT TGTACAAGCT GTTGTTCTGC CAACTATGGG TCTGGTGTCC 
CCCATAAGTA TTAACCTTAA TGACATTCAG AATGTTCTTA AGGTGGCAGT TGATGGGAAT 
GTAATAAGAC AAGTATTAGA AAATAACCAT GTCAGTCTTG CATCCAAAGA ACAAGGGACT 
CTCACCTCCG CCACAATCCA ACAAGCCGGC CATTCTGTTA TTTCCGCTAT TAGTCTTCCA 
TTGGTTGATC AAGACGGTAC AACCAAGATC ATCATCAATT ACAGCTTAGA GCAACCTAGT 
CAGCTTCCCC TCGTTGCGCA AAATTTGAAA AAAGAAGCCA CAGCTCCTGC AGAAGTCTGC 
AAAACGGAGA AGTTGCCAGA AGATCTCACT ATCAAAACTG AAAAGGATAC TAAGTTTACA 
GAAGAAAGCA CTTGTTTACT TGGTGCCGAT CACCCAGGAG ACAAAAATGC ACTTCAAGAA 
CCCAAGCACT ATGATCAGAA AAAGAACAGC CTACTTCCAG AGAGTAGCAA AGCCGAACCT 
ATCAAATCCG AGCCAACTTC TGCCGACCCC GAAGATGGCA ATCTTACTCC AAGCCAACCA 
CCTTTAAAGA ATCTCTTATC TCTTCTGAAA GCATACTATG CTTTAAATGC TCAGCCGAGC 
TCCGAGGAAC TTTCTAAAAT TGCAGATTCA GTAAACCTAC CACTCGACGT GGTAAAAGAC 
TGGTTTGAAA AAATGCAGAA TGGAGAAATA TCTGTGGAGT CATCTCGGCC CTCATCTCCA 
AAACCTGGAC AAGACAGTGT TACAGAAAAC AGTGTGGAAT CTAAAGATGA AAGTGGATTG 
CATAACGGCA CGAACGTTTC ACTGAAATCC CCCAAACAAG AACCTGAGGA ACCGTTAGCT 
GTTAATGGTC CAGAAAGTGG CACAGCTTCT CCTACACCAC TAAACCTCTC TTCTTTAAAA 
AACTCAGAAG GTGCAGCCTG CTCCGATGGA ACCCCAGATG GGGAGCAGCA GGCCGAGCCT 
CTTGATCTAT CCTTACCAAA GCCACATGGA AATGTGCCGG AGCGACCTTC CATCACTAGT 
GTTTACCAGA GCAGTTCATA TTCTGTTCAA GAAGAACCCT TGAACTTGTC TTGTGTTAAA 
AAAGAGCCAA TGCAAGTGGA CGATGTTACA AAAGCAGAGC CAATGCTAAT TATTAACCCA 
AGTGCCAATC CAATAAATAT TGCTATTCCT ACAGTCACTG CCCAGTTACC TACAATCGTT 
GCCATTGCCG ATCAAAACGG TGTTCCGTGT TTAAGAGCTC TAGCTGCCAG TAAGCGAACT 
ATCTTGATTC CCCAAGTGAC GTACACTTAC TCAACCACTG CTAATCCTTC AGTACCGGAA 
CCGCAACTCA AGAACATCCA GCCTAATGGA AATCAGGATG AGCGGCAGGA TACAAGCTCG 
GAAGGAGTGT CAAATGTAGA AGATCAGAAT GACTCTGATT CCACCCCACC CAAAAAGAAA 
CTGAAAAAAA CAGAGAACGG TCTCTATGCC TGTGACCTCT GTGACAAAAT TTTCCAGAAA 
AGCAGCTCAC TGCTGAGACA TAAGTATGAA CACACAGGTA AGAGGCCACA TGAATGTGGA 
ATCTGTGCAA AGGCATTTAA ACACAAACAC CATTTAATTG AGCATATGCG CCTACATTCT 
GGAGAAAAGC CCTATCAATG TGACAAATGT GGGAAACGGT TTTCGCACTC CGGATCCTAC 
TCTCAGCACA TGAACCACCG CTACTCTTAC TGCAAGAAGG AAGCCGAGGA ACGAGATGGC 
ATGGAGCAGG AAGAATCCGC TCAGGAGATC CTTGGCAACG AGAACGTGGA TTCCCAGGTG 
TCTCCATCTC AGATAGACTC TGATGAGCGC GAGAGCATAA CCCGGGAGGA AGACAGTGAG 
AAGGAAGAGG AGGATAAGGA AGAAGACGAT AAGGAAGAGG AGGATGAGAA AGAGGAGGAT 
AAGGAAGAGG AGGAAAAGGA AGAGGAGGAT GAGAAAGAGG AGGATGAGAA AGATATGGAT 
GATGGACAGG AAGAAAAGGA ACATGTTGGA GAGGGGGAAA TGGAAGAGGG AGAAGCAGAA 
CCATTGGCAG GTACTTTGAA GGATGAAGAA TGTGTAGAGG AAGACAAATG TTTGGAAGAA 
GAGGTCACAG AAGAGAATGT ATCGGAGAAA GCCCTCCCTC AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759228.1
CDS	810..3557
Translation	MCSYTFAYRTQLDRHMTSHKSGKDQRHVTQSGGNRKFKCPECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGVAPVNGRVRSGLKTPQCSSPSLSASPGSPARPQIRQKIENKPLQEQIPVNQIKTEPVDYEIKPIVVTSGINCSAPLQNGVFSSGSRLQATNSPQGVVQAVVLPTMGLVSPISINLNDIQNVLKVAVDGNVIRQVLENNHVSLASKEQGTLTSATIQQAGHSVISAISLPLVDQDGTTKIIINYSLEQPSQLPLVAQNLKKEATAPAEVCKTEKLPEDLTIKTEKDTKFTEESTCLLGADHPGDKNALQEPKHYDQKKNSLLPESSKAEPIKSEPTSADPEDGNLTPSQPPLKNLLSLLKAYYALNAQPSSEELSKIADSVNLPLDVVKDWFEKMQNGEISVESSRPSSPKPGQDSVTENSVESKDESGLHNGTNVSLKSPKQEPEEPLAVNGPESGTASPTPLNLSSLKNSEGAACSDGTPDGEQQAEPLDLSLPKPHGNVPERPSITSVYQSSSYSVQEEPLNLSCVKKEPMQVDDVTKAEPMLIINPSANPINIAIPTVTAQLPTIVAIADQNGVPCLRALAASKRTILIPQVTYTYSTTANPSVPEPQLKNIQPNGNQDERQDTSSEGVSNVEDQNDSDSTPPKKKLKKTENGLYACDLCDKIFQKSSSLLRHKYEHTGKRPHECGICAKAFKHKHHLIEHMRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKKEAEERDGMEQEESAQEILGNENVDSQVSPSQIDSDERESITREEDSEKEEEDKEEDDKEEEDEKEEDKEEEEKEEEDEKEEDEKDMDDGQEEKEHVGEGEMEEGEAEPLAGTLKDEECVEEDKCLEEEVTEENVSEKALPQP

Target ORF information:

RefSeq Version	XM_031903368.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903368.1

ORF Insert Sequence:

ATGTGTAGTT ATACCTTTGC TTATAGAACC CAGCTTGATC GTCACATGAC CTCTCATAAA 
TCAGGAAAAG ATCAGAGACA TGTGACGCAG TCTGGAGGCA ACCGTAAATT CAAATGCCCT 
GAGTGTGGAA AAGCTTTTAA ATACAAACAC CATCTGAAAG AACACTTACG GATTCACAGT 
GGAGAGAAGC CATACGAGTG CCCAAACTGC AAGAAGCGAT TTTCTCATTC TGGTTCCTAC 
AGTTCCCACA TAAGCAGTAA GAAATGTATC GGTGTGGCGC CTGTGAATGG ACGCGTGCGC 
TCAGGGCTGA AGACTCCCCA GTGCTCCTCC CCGTCCCTTT CTGCTTCTCC GGGAAGTCCA 
GCTCGACCAC AGATCCGCCA GAAGATAGAG AACAAACCTT TGCAAGAGCA GATTCCAGTT 
AACCAAATCA AAACCGAACC TGTGGATTAT GAAATCAAGC CTATCGTGGT GACATCAGGA 
ATCAACTGCT CAGCCCCTTT GCAAAATGGG GTGTTTAGTA GTGGTAGCCG TTTGCAGGCA 
ACTAATTCTC CTCAGGGTGT TGTACAAGCT GTTGTTCTGC CAACTATGGG TCTGGTGTCC 
CCCATAAGTA TTAACCTTAA TGACATTCAG AATGTTCTTA AGGTGGCAGT TGATGGGAAT 
GTAATAAGAC AAGTATTAGA AAATAACCAT GTCAGTCTTG CATCCAAAGA ACAAGGGACT 
CTCACCTCCG CCACAATCCA ACAAGCCGGC CATTCTGTTA TTTCCGCTAT TAGTCTTCCA 
TTGGTTGATC AAGACGGTAC AACCAAGATC ATCATCAATT ACAGCTTAGA GCAACCTAGT 
CAGCTTCCCC TCGTTGCGCA AAATTTGAAA AAAGAAGCCA CAGCTCCTGC AGAAGTCTGC 
AAAACGGAGA AGTTGCCAGA AGATCTCACT ATCAAAACTG AAAAGGATAC TAAGTTTACA 
GAAGAAAGCA CTTGTTTACT TGGTGCCGAT CACCCAGGAG ACAAAAATGC ACTTCAAGAA 
CCCAAGCACT ATGATCAGAA AAAGAACAGC CTACTTCCAG AGAGTAGCAA AGCCGAACCT 
ATCAAATCCG AGCCAACTTC TGCCGACCCC GAAGATGGCA ATCTTACTCC AAGCCAACCA 
CCTTTAAAGA ATCTCTTATC TCTTCTGAAA GCATACTATG CTTTAAATGC TCAGCCGAGC 
TCCGAGGAAC TTTCTAAAAT TGCAGATTCA GTAAACCTAC CACTCGACGT GGTAAAAGAC 
TGGTTTGAAA AAATGCAGAA TGGAGAAATA TCTGTGGAGT CATCTCGGCC CTCATCTCCA 
AAACCTGGAC AAGACAGTGT TACAGAAAAC AGTGTGGAAT CTAAAGATGA AAGTGGATTG 
CATAACGGCA CGAACGTTTC ACTGAAATCC CCCAAACAAG AACCTGAGGA ACCGTTAGCT 
GTTAATGGTC CAGAAAGTGG CACAGCTTCT CCTACACCAC TAAACCTCTC TTCTTTAAAA 
AACTCAGAAG GTGCAGCCTG CTCCGATGGA ACCCCAGATG GGGAGCAGCA GGCCGAGCCT 
CTTGATCTAT CCTTACCAAA GCCACATGGA AATGTGCCGG AGCGACCTTC CATCACTAGT 
GTTTACCAGA GCAGTTCATA TTCTGTTCAA GAAGAACCCT TGAACTTGTC TTGTGTTAAA 
AAAGAGCCAA TGCAAGTGGA CGATGTTACA AAAGCAGAGC CAATGCTAAT TATTAACCCA 
AGTGCCAATC CAATAAATAT TGCTATTCCT ACAGTCACTG CCCAGTTACC TACAATCGTT 
GCCATTGCCG ATCAAAACGG TGTTCCGTGT TTAAGAGCTC TAGCTGCCAG TAAGCGAACT 
ATCTTGATTC CCCAAGTGAC GTACACTTAC TCAACCACTG CTAATCCTTC AGTACCGGAA 
CCGCAACTCA AGAACATCCA GCCTAATGGA AATCAGGATG AGCGGCAGGA TACAAGCTCG 
GAAGGAGTGT CAAATGTAGA AGATCAGAAT GACTCTGATT CCACCCCACC CAAAAAGAAA 
CTGAAAAAAA CAGAGAACGG TCTCTATGCC TGTGACCTCT GTGACAAAAT TTTCCAGAAA 
AGCAGCTCAC TGCTGAGACA TAAGTATGAA CACACAGGTA AGAGGCCACA TGAATGTGGA 
ATCTGTGCAA AGGCATTTAA ACACAAACAC CATTTAATTG AGCATATGCG CCTACATTCT 
GGAGAAAAGC CCTATCAATG TGACAAATGT GGGAAACGGT TTTCGCACTC CGGATCCTAC 
TCTCAGCACA TGAACCACCG CTACTCTTAC TGCAAGAAGG AAGCCGAGGA ACGAGATGGC 
ATGGAGCAGG AAGAATCCGC TCAGGAGATC CTTGGCAACG AGAACGTGGA TTCCCAGGTG 
TCTCCATCTC AGATAGACTC TGATGAGCGC GAGAGCATAA CCCGGGAGGA AGACAGTGAG 
AAGGAAGAGG AGGATAAGGA AGAAGACGAT AAGGAAGAGG AGGATGAGAA AGAGGAGGAT 
AAGGAAGAGG AGGAAAAGGA AGAGGAGGAT GAGAAAGAGG AGGATGAGAA AGATATGGAT 
GATGGACAGG AAGAAAAGGA ACATGTTGGA GAGGGGGAAA TGGAAGAGGG AGAAGCAGAA 
CCATTGGCAG GTACTTTGAA GGATGAAGAA TGTGTAGAGG AAGACAAATG TTTGGAAGAA 
GAGGTCACAG AAGAGAATGT ATCGGAGAAA GCCCTCCCTC AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zeb1 ( XM_012964650.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820104.1 Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa59678
Clone ID Related Accession (Same CDS sequence)	XM_012964650.3
Accession Version	XM_012964650.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger E-box-binding homeobox 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964650.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG GCCCCAGGTG TAAGCGCAGA AAACAGGCGA ACCCCAGACG GAATAACGTT 
ACCAATTACA ACAGTGTGCT CGAAGCAAAT TCTGATTCGG ATGATGAAGA CAAATTGCAC 
ATAGTGGAAG AGGAAAGTGT TACAGACGCA GCAGATGGCG AGGGCAGCGT TCCAGAAGAT 
GACTTGCCAA CAGACCAAAC AGTATTACCA GAAGGCAGCG AGAGCGCAAA GGGGAGCGGC 
AAAACTTGCT GGGAAGATGA AGAAGGGGCA GATTGCAGTG ATGAGATCCT GGGGCCTGAA 
GCCGAAGCTG ATGAACTTGG ATGTACAGTT AAAGATGAAG AATGCGATTC AGACCTAGAA 
AATGAACAAA ACCAAGATCC CAACGTTGAA GAATTCCTAC AGCAAAGCGA CACTGCTGTA 
ATCTATCCGG AAGCGCCAGA GGATGACCAG AGGCAAGGCA CGCCGGAGGC GGTTGGTCAA 
GATGAAAATG GAACGCCAGA CGCATTCTCC CAGCTCCTCA CCTGCCCGTA TTGTGACAGG 
GGATACAAGC GCTTCACGTC TTTGAAGGAA CATATTAAGT ACCGCCATGA GAAAAATGAA 
GACAATTTCA GTTGCTCGAT GTGTAGTTAT ACCTTTGCTT ATAGAACCCA GCTTGATCGT 
CACATGACCT CTCATAAATC AGGAAAAGAT CAGAGACATG TGACGCAGTC TGGAGGCAAC 
CGTAAATTCA AATGCCCTGA GTGTGGAAAA GCTTTTAAAT ACAAACACCA TCTGAAAGAA 
CACTTACGGA TTCACAGTGG AGAGAAGCCA TACGAGTGCC CAAACTGCAA GAAGCGATTT 
TCTCATTCTG GTTCCTACAG TTCCCACATA AGCAGTAAGA AATGTATCGG TGTGGCGCCT 
GTGAATGGAC GCGTGCGCTC AGGGCTGAAG ACTCCCCAGT GCTCCTCCCC GTCCCTTTCT 
GCTTCTCCGG GAAGTCCAGC TCGACCACAG ATCCGCCAGA AGATAGAGAA CAAACCTTTG 
CAAGAGCAGA TTCCAGTTAA CCAAATCAAA ACCGAACCTG TGGATTATGA AATCAAGCCT 
ATCGTGGTGA CATCAGGAAT CAACTGCTCA GCCCCTTTGC AAAATGGGGT GTTTAGTAGT 
GGTAGCCGTT TGCAGGCAAC TAATTCTCCT CAGGGTGTTG TACAAGCTGT TGTTCTGCCA 
ACTATGGGTC TGGTGTCCCC CATAAGTATT AACCTTAATG ACATTCAGAA TGTTCTTAAG 
GTGGCAGTTG ATGGGAATGT AATAAGACAA GTATTAGAAA ATAACCATGT CAGTCTTGCA 
TCCAAAGAAC AAGGGACTCT CACCTCCGCC ACAATCCAAC AAGCCGGCCA TTCTGTTATT 
TCCGCTATTA GTCTTCCATT GGTTGATCAA GACGGTACAA CCAAGATCAT CATCAATTAC 
AGCTTAGAGC AACCTAGTCA GCTTCCCCTC GTTGCGCAAA ATTTGAAAAA AGAAGCCACA 
GCTCCTGCAG AAGTCTGCAA AACGGAGAAG TTGCCAGAAG ATCTCACTAT CAAAACTGAA 
AAGGATACTA AGTTTACAGA AGAAAGCACT TGTTTACTTG GTGCCGATCA CCCAGGAGAC 
AAAAATGCAC TTCAAGAACC CAAGCACTAT GATCAGAAAA AGAACAGCCT ACTTCCAGAG 
AGTAGCAAAG CCGAACCTAT CAAATCCGAG CCAACTTCTG CCGACCCCGA AGATGGCAAT 
CTTACTCCAA GCCAACCACC TTTAAAGAAT CTCTTATCTC TTCTGAAAGC ATACTATGCT 
TTAAATGCTC AGCCGAGCTC CGAGGAACTT TCTAAAATTG CAGATTCAGT AAACCTACCA 
CTCGACGTGG TAAAAGACTG GTTTGAAAAA ATGCAGAATG GAGAAATATC TGTGGAGTCA 
TCTCGGCCCT CATCTCCAAA ACCTGGACAA GACAGTGTTA CAGAAAACAG TGTGGAATCT 
AAAGATGAAA GTGGATTGCA TAACGGCACG AACGTTTCAC TGAAATCCCC CAAACAAGAA 
CCTGAGGAAC CGTTAGCTGT TAATGGTCCA GAAAGTGGCA CAGCTTCTCC TACACCACTA 
AACCTCTCTT CTTTAAAAAA CTCAGAAGGT GCAGCCTGCT CCGATGGAAC CCCAGATGGG 
GAGCAGCAGG CCGAGCCTCT TGATCTATCC TTACCAAAGC CACATGGAAA TGTGCCGGAG 
CGACCTTCCA TCACTAGTGT TTACCAGAGC AGTTCATATT CTGTTCAAGA AGAACCCTTG 
AACTTGTCTT GTGTTAAAAA AGAGCCAATG CAAGTGGACG ATGTTACAAA AGCAGAGCCA 
ATGCTAATTA TTAACCCAAG TGCCAATCCA ATAAATATTG CTATTCCTAC AGTCACTGCC 
CAGTTACCTA CAATCGTTGC CATTGCCGAT CAAAACGGTG TTCCGTGTTT AAGAGCTCTA 
GCTGCCAGTA AGCGAACTAT CTTGATTCCC CAAGTGACGT ACACTTACTC AACCACTGCT 
AATCCTTCAG TACCGGAACC GCAACTCAAG AACATCCAGC CTAATGGAAA TCAGGATGAG 
CGGCAGGATA CAAGCTCGGA AGGAGTGTCA AATGTAGAAG ATCAGAATGA CTCTGATTCC 
ACCCCACCCA AAAAGAAACT GAAAAAAACA GAGAACGGTC TCTATGCCTG TGACCTCTGT 
GACAAAATTT TCCAGAAAAG CAGCTCACTG CTGAGACATA AGTATGAACA CACAGGTAAG 
AGGCCACATG AATGTGGAAT CTGTGCAAAG GCATTTAAAC ACAAACACCA TTTAATTGAG 
CATATGCGCC TACATTCTGG AGAAAAGCCC TATCAATGTG ACAAATGTGG GAAACGGTTT 
TCGCACTCCG GATCCTACTC TCAGCACATG AACCACCGCT ACTCTTACTG CAAGAAGGAA 
GCCGAGGAAC GAGATGGCAT GGAGCAGGAA GAATCCGCTC AGGAGATCCT TGGCAACGAG 
AACGTGGATT CCCAGGTGTC TCCATCTCAG ATAGACTCTG ATGAGCGCGA GAGCATAACC 
CGGGAGGAAG ACAGTGAGAA GGAAGAGGAG GATAAGGAAG AAGACGATAA GGAAGAGGAG 
GATGAGAAAG AGGAGGATAA GGAAGAGGAG GAAAAGGAAG AGGAGGATGA GAAAGAGGAG 
GATGAGAAAG ATATGGATGA TGGACAGGAA GAAAAGGAAC ATGTTGGAGA GGGGGAAATG 
GAAGAGGGAG AAGCAGAACC ATTGGCAGGT ACTTTGAAGG ATGAAGAATG TGTAGAGGAA 
GACAAATGTT TGGAAGAAGA GGTCACAGAA GAGAATGTAT CGGAGAAAGC CCTCCCTCAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820104.1
CDS	46..3411
Translation	MADGPRCKRRKQANPRRNNVTNYNSVLEANSDSDDEDKLHIVEEESVTDAADGEGSVPEDDLPTDQTVLPEGSESAKGSGKTCWEDEEGADCSDEILGPEAEADELGCTVKDEECDSDLENEQNQDPNVEEFLQQSDTAVIYPEAPEDDQRQGTPEAVGQDENGTPDAFSQLLTCPYCDRGYKRFTSLKEHIKYRHEKNEDNFSCSMCSYTFAYRTQLDRHMTSHKSGKDQRHVTQSGGNRKFKCPECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGVAPVNGRVRSGLKTPQCSSPSLSASPGSPARPQIRQKIENKPLQEQIPVNQIKTEPVDYEIKPIVVTSGINCSAPLQNGVFSSGSRLQATNSPQGVVQAVVLPTMGLVSPISINLNDIQNVLKVAVDGNVIRQVLENNHVSLASKEQGTLTSATIQQAGHSVISAISLPLVDQDGTTKIIINYSLEQPSQLPLVAQNLKKEATAPAEVCKTEKLPEDLTIKTEKDTKFTEESTCLLGADHPGDKNALQEPKHYDQKKNSLLPESSKAEPIKSEPTSADPEDGNLTPSQPPLKNLLSLLKAYYALNAQPSSEELSKIADSVNLPLDVVKDWFEKMQNGEISVESSRPSSPKPGQDSVTENSVESKDESGLHNGTNVSLKSPKQEPEEPLAVNGPESGTASPTPLNLSSLKNSEGAACSDGTPDGEQQAEPLDLSLPKPHGNVPERPSITSVYQSSSYSVQEEPLNLSCVKKEPMQVDDVTKAEPMLIINPSANPINIAIPTVTAQLPTIVAIADQNGVPCLRALAASKRTILIPQVTYTYSTTANPSVPEPQLKNIQPNGNQDERQDTSSEGVSNVEDQNDSDSTPPKKKLKKTENGLYACDLCDKIFQKSSSLLRHKYEHTGKRPHECGICAKAFKHKHHLIEHMRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKKEAEERDGMEQEESAQEILGNENVDSQVSPSQIDSDERESITREEDSEKEEEDKEEDDKEEEDEKEEDKEEEEKEEEDEKEEDEKDMDDGQEEKEHVGEGEMEEGEAEPLAGTLKDEECVEEDKCLEEEVTEENVSEKALPQP

Target ORF information:

RefSeq Version	XM_012964650.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964650.3

ORF Insert Sequence:

ATGGCGGATG GCCCCAGGTG TAAGCGCAGA AAACAGGCGA ACCCCAGACG GAATAACGTT 
ACCAATTACA ACAGTGTGCT CGAAGCAAAT TCTGATTCGG ATGATGAAGA CAAATTGCAC 
ATAGTGGAAG AGGAAAGTGT TACAGACGCA GCAGATGGCG AGGGCAGCGT TCCAGAAGAT 
GACTTGCCAA CAGACCAAAC AGTATTACCA GAAGGCAGCG AGAGCGCAAA GGGGAGCGGC 
AAAACTTGCT GGGAAGATGA AGAAGGGGCA GATTGCAGTG ATGAGATCCT GGGGCCTGAA 
GCCGAAGCTG ATGAACTTGG ATGTACAGTT AAAGATGAAG AATGCGATTC AGACCTAGAA 
AATGAACAAA ACCAAGATCC CAACGTTGAA GAATTCCTAC AGCAAAGCGA CACTGCTGTA 
ATCTATCCGG AAGCGCCAGA GGATGACCAG AGGCAAGGCA CGCCGGAGGC GGTTGGTCAA 
GATGAAAATG GAACGCCAGA CGCATTCTCC CAGCTCCTCA CCTGCCCGTA TTGTGACAGG 
GGATACAAGC GCTTCACGTC TTTGAAGGAA CATATTAAGT ACCGCCATGA GAAAAATGAA 
GACAATTTCA GTTGCTCGAT GTGTAGTTAT ACCTTTGCTT ATAGAACCCA GCTTGATCGT 
CACATGACCT CTCATAAATC AGGAAAAGAT CAGAGACATG TGACGCAGTC TGGAGGCAAC 
CGTAAATTCA AATGCCCTGA GTGTGGAAAA GCTTTTAAAT ACAAACACCA TCTGAAAGAA 
CACTTACGGA TTCACAGTGG AGAGAAGCCA TACGAGTGCC CAAACTGCAA GAAGCGATTT 
TCTCATTCTG GTTCCTACAG TTCCCACATA AGCAGTAAGA AATGTATCGG TGTGGCGCCT 
GTGAATGGAC GCGTGCGCTC AGGGCTGAAG ACTCCCCAGT GCTCCTCCCC GTCCCTTTCT 
GCTTCTCCGG GAAGTCCAGC TCGACCACAG ATCCGCCAGA AGATAGAGAA CAAACCTTTG 
CAAGAGCAGA TTCCAGTTAA CCAAATCAAA ACCGAACCTG TGGATTATGA AATCAAGCCT 
ATCGTGGTGA CATCAGGAAT CAACTGCTCA GCCCCTTTGC AAAATGGGGT GTTTAGTAGT 
GGTAGCCGTT TGCAGGCAAC TAATTCTCCT CAGGGTGTTG TACAAGCTGT TGTTCTGCCA 
ACTATGGGTC TGGTGTCCCC CATAAGTATT AACCTTAATG ACATTCAGAA TGTTCTTAAG 
GTGGCAGTTG ATGGGAATGT AATAAGACAA GTATTAGAAA ATAACCATGT CAGTCTTGCA 
TCCAAAGAAC AAGGGACTCT CACCTCCGCC ACAATCCAAC AAGCCGGCCA TTCTGTTATT 
TCCGCTATTA GTCTTCCATT GGTTGATCAA GACGGTACAA CCAAGATCAT CATCAATTAC 
AGCTTAGAGC AACCTAGTCA GCTTCCCCTC GTTGCGCAAA ATTTGAAAAA AGAAGCCACA 
GCTCCTGCAG AAGTCTGCAA AACGGAGAAG TTGCCAGAAG ATCTCACTAT CAAAACTGAA 
AAGGATACTA AGTTTACAGA AGAAAGCACT TGTTTACTTG GTGCCGATCA CCCAGGAGAC 
AAAAATGCAC TTCAAGAACC CAAGCACTAT GATCAGAAAA AGAACAGCCT ACTTCCAGAG 
AGTAGCAAAG CCGAACCTAT CAAATCCGAG CCAACTTCTG CCGACCCCGA AGATGGCAAT 
CTTACTCCAA GCCAACCACC TTTAAAGAAT CTCTTATCTC TTCTGAAAGC ATACTATGCT 
TTAAATGCTC AGCCGAGCTC CGAGGAACTT TCTAAAATTG CAGATTCAGT AAACCTACCA 
CTCGACGTGG TAAAAGACTG GTTTGAAAAA ATGCAGAATG GAGAAATATC TGTGGAGTCA 
TCTCGGCCCT CATCTCCAAA ACCTGGACAA GACAGTGTTA CAGAAAACAG TGTGGAATCT 
AAAGATGAAA GTGGATTGCA TAACGGCACG AACGTTTCAC TGAAATCCCC CAAACAAGAA 
CCTGAGGAAC CGTTAGCTGT TAATGGTCCA GAAAGTGGCA CAGCTTCTCC TACACCACTA 
AACCTCTCTT CTTTAAAAAA CTCAGAAGGT GCAGCCTGCT CCGATGGAAC CCCAGATGGG 
GAGCAGCAGG CCGAGCCTCT TGATCTATCC TTACCAAAGC CACATGGAAA TGTGCCGGAG 
CGACCTTCCA TCACTAGTGT TTACCAGAGC AGTTCATATT CTGTTCAAGA AGAACCCTTG 
AACTTGTCTT GTGTTAAAAA AGAGCCAATG CAAGTGGACG ATGTTACAAA AGCAGAGCCA 
ATGCTAATTA TTAACCCAAG TGCCAATCCA ATAAATATTG CTATTCCTAC AGTCACTGCC 
CAGTTACCTA CAATCGTTGC CATTGCCGAT CAAAACGGTG TTCCGTGTTT AAGAGCTCTA 
GCTGCCAGTA AGCGAACTAT CTTGATTCCC CAAGTGACGT ACACTTACTC AACCACTGCT 
AATCCTTCAG TACCGGAACC GCAACTCAAG AACATCCAGC CTAATGGAAA TCAGGATGAG 
CGGCAGGATA CAAGCTCGGA AGGAGTGTCA AATGTAGAAG ATCAGAATGA CTCTGATTCC 
ACCCCACCCA AAAAGAAACT GAAAAAAACA GAGAACGGTC TCTATGCCTG TGACCTCTGT 
GACAAAATTT TCCAGAAAAG CAGCTCACTG CTGAGACATA AGTATGAACA CACAGGTAAG 
AGGCCACATG AATGTGGAAT CTGTGCAAAG GCATTTAAAC ACAAACACCA TTTAATTGAG 
CATATGCGCC TACATTCTGG AGAAAAGCCC TATCAATGTG ACAAATGTGG GAAACGGTTT 
TCGCACTCCG GATCCTACTC TCAGCACATG AACCACCGCT ACTCTTACTG CAAGAAGGAA 
GCCGAGGAAC GAGATGGCAT GGAGCAGGAA GAATCCGCTC AGGAGATCCT TGGCAACGAG 
AACGTGGATT CCCAGGTGTC TCCATCTCAG ATAGACTCTG ATGAGCGCGA GAGCATAACC 
CGGGAGGAAG ACAGTGAGAA GGAAGAGGAG GATAAGGAAG AAGACGATAA GGAAGAGGAG 
GATGAGAAAG AGGAGGATAA GGAAGAGGAG GAAAAGGAAG AGGAGGATGA GAAAGAGGAG 
GATGAGAAAG ATATGGATGA TGGACAGGAA GAAAAGGAAC ATGTTGGAGA GGGGGAAATG 
GAAGAGGGAG AAGCAGAACC ATTGGCAGGT ACTTTGAAGG ATGAAGAATG TGTAGAGGAA 
GACAAATGTT TGGAAGAAGA GGTCACAGAA GAGAATGTAT CGGAGAAAGC CCTCCCTCAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zeb1 ( XM_031903367.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759227.1 Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), transcript variant X2, mRNA.

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CloneID	OXa59679
Clone ID Related Accession (Same CDS sequence)	XM_031903367.1
Accession Version	XM_031903367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger E-box-binding homeobox 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTT GTGCAGTTAC CAATTACAAC AGTGTGCTCG AAGCAAATTC TGATTCGGAT 
GATGAAGACA AATTGCACAT AGTGGAAGAG GAAAGTGTTA CAGACGCAGC AGATGGCGAG 
GGCAGCGTTC CAGAAGATGA CTTGCCAACA GACCAAACAG TATTACCAGA AGGCAGCGAG 
AGCGCAAAGG GGAGCGGCAA AACTTGCTGG GAAGATGAAG AAGGGGCAGA TTGCAGTGAT 
GAGATCCTGG GGCCTGAAGC CGAAGCTGAT GAACTTGGAT GTACAGTTAA AGATGAAGAA 
TGCGATTCAG ACCTAGAAAA TGAACAAAAC CAAGATCCCA ACGTTGAAGA ATTCCTACAG 
CAAAGCGACA CTGCTGTAAT CTATCCGGAA GCGCCAGAGG ATGACCAGAG GCAAGGCACG 
CCGGAGGCGG TTGGTCAAGA TGAAAATGGA ACGCCAGACG CATTCTCCCA GCTCCTCACC 
TGCCCGTATT GTGACAGGGG ATACAAGCGC TTCACGTCTT TGAAGGAACA TATTAAGTAC 
CGCCATGAGA AAAATGAAGA CAATTTCAGT TGCTCGATGT GTAGTTATAC CTTTGCTTAT 
AGAACCCAGC TTGATCGTCA CATGACCTCT CATAAATCAG GAAAAGATCA GAGACATGTG 
ACGCAGTCTG GAGGCAACCG TAAATTCAAA TGCCCTGAGT GTGGAAAAGC TTTTAAATAC 
AAACACCATC TGAAAGAACA CTTACGGATT CACAGTGGAG AGAAGCCATA CGAGTGCCCA 
AACTGCAAGA AGCGATTTTC TCATTCTGGT TCCTACAGTT CCCACATAAG CAGTAAGAAA 
TGTATCGGTG TGGCGCCTGT GAATGGACGC GTGCGCTCAG GGCTGAAGAC TCCCCAGTGC 
TCCTCCCCGT CCCTTTCTGC TTCTCCGGGA AGTCCAGCTC GACCACAGAT CCGCCAGAAG 
ATAGAGAACA AACCTTTGCA AGAGCAGATT CCAGTTAACC AAATCAAAAC CGAACCTGTG 
GATTATGAAA TCAAGCCTAT CGTGGTGACA TCAGGAATCA ACTGCTCAGC CCCTTTGCAA 
AATGGGGTGT TTAGTAGTGG TAGCCGTTTG CAGGCAACTA ATTCTCCTCA GGGTGTTGTA 
CAAGCTGTTG TTCTGCCAAC TATGGGTCTG GTGTCCCCCA TAAGTATTAA CCTTAATGAC 
ATTCAGAATG TTCTTAAGGT GGCAGTTGAT GGGAATGTAA TAAGACAAGT ATTAGAAAAT 
AACCATGTCA GTCTTGCATC CAAAGAACAA GGGACTCTCA CCTCCGCCAC AATCCAACAA 
GCCGGCCATT CTGTTATTTC CGCTATTAGT CTTCCATTGG TTGATCAAGA CGGTACAACC 
AAGATCATCA TCAATTACAG CTTAGAGCAA CCTAGTCAGC TTCCCCTCGT TGCGCAAAAT 
TTGAAAAAAG AAGCCACAGC TCCTGCAGAA GTCTGCAAAA CGGAGAAGTT GCCAGAAGAT 
CTCACTATCA AAACTGAAAA GGATACTAAG TTTACAGAAG AAAGCACTTG TTTACTTGGT 
GCCGATCACC CAGGAGACAA AAATGCACTT CAAGAACCCA AGCACTATGA TCAGAAAAAG 
AACAGCCTAC TTCCAGAGAG TAGCAAAGCC GAACCTATCA AATCCGAGCC AACTTCTGCC 
GACCCCGAAG ATGGCAATCT TACTCCAAGC CAACCACCTT TAAAGAATCT CTTATCTCTT 
CTGAAAGCAT ACTATGCTTT AAATGCTCAG CCGAGCTCCG AGGAACTTTC TAAAATTGCA 
GATTCAGTAA ACCTACCACT CGACGTGGTA AAAGACTGGT TTGAAAAAAT GCAGAATGGA 
GAAATATCTG TGGAGTCATC TCGGCCCTCA TCTCCAAAAC CTGGACAAGA CAGTGTTACA 
GAAAACAGTG TGGAATCTAA AGATGAAAGT GGATTGCATA ACGGCACGAA CGTTTCACTG 
AAATCCCCCA AACAAGAACC TGAGGAACCG TTAGCTGTTA ATGGTCCAGA AAGTGGCACA 
GCTTCTCCTA CACCACTAAA CCTCTCTTCT TTAAAAAACT CAGAAGGTGC AGCCTGCTCC 
GATGGAACCC CAGATGGGGA GCAGCAGGCC GAGCCTCTTG ATCTATCCTT ACCAAAGCCA 
CATGGAAATG TGCCGGAGCG ACCTTCCATC ACTAGTGTTT ACCAGAGCAG TTCATATTCT 
GTTCAAGAAG AACCCTTGAA CTTGTCTTGT GTTAAAAAAG AGCCAATGCA AGTGGACGAT 
GTTACAAAAG CAGAGCCAAT GCTAATTATT AACCCAAGTG CCAATCCAAT AAATATTGCT 
ATTCCTACAG TCACTGCCCA GTTACCTACA ATCGTTGCCA TTGCCGATCA AAACGGTGTT 
CCGTGTTTAA GAGCTCTAGC TGCCAGTAAG CGAACTATCT TGATTCCCCA AGTGACGTAC 
ACTTACTCAA CCACTGCTAA TCCTTCAGTA CCGGAACCGC AACTCAAGAA CATCCAGCCT 
AATGGAAATC AGGATGAGCG GCAGGATACA AGCTCGGAAG GAGTGTCAAA TGTAGAAGAT 
CAGAATGACT CTGATTCCAC CCCACCCAAA AAGAAACTGA AAAAAACAGA GAACGGTCTC 
TATGCCTGTG ACCTCTGTGA CAAAATTTTC CAGAAAAGCA GCTCACTGCT GAGACATAAG 
TATGAACACA CAGGTAAGAG GCCACATGAA TGTGGAATCT GTGCAAAGGC ATTTAAACAC 
AAACACCATT TAATTGAGCA TATGCGCCTA CATTCTGGAG AAAAGCCCTA TCAATGTGAC 
AAATGTGGGA AACGGTTTTC GCACTCCGGA TCCTACTCTC AGCACATGAA CCACCGCTAC 
TCTTACTGCA AGAAGGAAGC CGAGGAACGA GATGGCATGG AGCAGGAAGA ATCCGCTCAG 
GAGATCCTTG GCAACGAGAA CGTGGATTCC CAGGTGTCTC CATCTCAGAT AGACTCTGAT 
GAGCGCGAGA GCATAACCCG GGAGGAAGAC AGTGAGAAGG AAGAGGAGGA TAAGGAAGAA 
GACGATAAGG AAGAGGAGGA TGAGAAAGAG GAGGATAAGG AAGAGGAGGA AAAGGAAGAG 
GAGGATGAGA AAGAGGAGGA TGAGAAAGAT ATGGATGATG GACAGGAAGA AAAGGAACAT 
GTTGGAGAGG GGGAAATGGA AGAGGGAGAA GCAGAACCAT TGGCAGGTAC TTTGAAGGAT 
GAAGAATGTG TAGAGGAAGA CAAATGTTTG GAAGAAGAGG TCACAGAAGA GAATGTATCG 
GAGAAAGCCC TCCCTCAGCC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759227.1
CDS	230..3553
Translation	MATCAVTNYNSVLEANSDSDDEDKLHIVEEESVTDAADGEGSVPEDDLPTDQTVLPEGSESAKGSGKTCWEDEEGADCSDEILGPEAEADELGCTVKDEECDSDLENEQNQDPNVEEFLQQSDTAVIYPEAPEDDQRQGTPEAVGQDENGTPDAFSQLLTCPYCDRGYKRFTSLKEHIKYRHEKNEDNFSCSMCSYTFAYRTQLDRHMTSHKSGKDQRHVTQSGGNRKFKCPECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGVAPVNGRVRSGLKTPQCSSPSLSASPGSPARPQIRQKIENKPLQEQIPVNQIKTEPVDYEIKPIVVTSGINCSAPLQNGVFSSGSRLQATNSPQGVVQAVVLPTMGLVSPISINLNDIQNVLKVAVDGNVIRQVLENNHVSLASKEQGTLTSATIQQAGHSVISAISLPLVDQDGTTKIIINYSLEQPSQLPLVAQNLKKEATAPAEVCKTEKLPEDLTIKTEKDTKFTEESTCLLGADHPGDKNALQEPKHYDQKKNSLLPESSKAEPIKSEPTSADPEDGNLTPSQPPLKNLLSLLKAYYALNAQPSSEELSKIADSVNLPLDVVKDWFEKMQNGEISVESSRPSSPKPGQDSVTENSVESKDESGLHNGTNVSLKSPKQEPEEPLAVNGPESGTASPTPLNLSSLKNSEGAACSDGTPDGEQQAEPLDLSLPKPHGNVPERPSITSVYQSSSYSVQEEPLNLSCVKKEPMQVDDVTKAEPMLIINPSANPINIAIPTVTAQLPTIVAIADQNGVPCLRALAASKRTILIPQVTYTYSTTANPSVPEPQLKNIQPNGNQDERQDTSSEGVSNVEDQNDSDSTPPKKKLKKTENGLYACDLCDKIFQKSSSLLRHKYEHTGKRPHECGICAKAFKHKHHLIEHMRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKKEAEERDGMEQEESAQEILGNENVDSQVSPSQIDSDERESITREEDSEKEEEDKEEDDKEEEDEKEEDKEEEEKEEEDEKEEDEKDMDDGQEEKEHVGEGEMEEGEAEPLAGTLKDEECVEEDKCLEEEVTEENVSEKALPQP

Target ORF information:

RefSeq Version	XM_031903367.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903367.1

ORF Insert Sequence:

ATGGCAACTT GTGCAGTTAC CAATTACAAC AGTGTGCTCG AAGCAAATTC TGATTCGGAT 
GATGAAGACA AATTGCACAT AGTGGAAGAG GAAAGTGTTA CAGACGCAGC AGATGGCGAG 
GGCAGCGTTC CAGAAGATGA CTTGCCAACA GACCAAACAG TATTACCAGA AGGCAGCGAG 
AGCGCAAAGG GGAGCGGCAA AACTTGCTGG GAAGATGAAG AAGGGGCAGA TTGCAGTGAT 
GAGATCCTGG GGCCTGAAGC CGAAGCTGAT GAACTTGGAT GTACAGTTAA AGATGAAGAA 
TGCGATTCAG ACCTAGAAAA TGAACAAAAC CAAGATCCCA ACGTTGAAGA ATTCCTACAG 
CAAAGCGACA CTGCTGTAAT CTATCCGGAA GCGCCAGAGG ATGACCAGAG GCAAGGCACG 
CCGGAGGCGG TTGGTCAAGA TGAAAATGGA ACGCCAGACG CATTCTCCCA GCTCCTCACC 
TGCCCGTATT GTGACAGGGG ATACAAGCGC TTCACGTCTT TGAAGGAACA TATTAAGTAC 
CGCCATGAGA AAAATGAAGA CAATTTCAGT TGCTCGATGT GTAGTTATAC CTTTGCTTAT 
AGAACCCAGC TTGATCGTCA CATGACCTCT CATAAATCAG GAAAAGATCA GAGACATGTG 
ACGCAGTCTG GAGGCAACCG TAAATTCAAA TGCCCTGAGT GTGGAAAAGC TTTTAAATAC 
AAACACCATC TGAAAGAACA CTTACGGATT CACAGTGGAG AGAAGCCATA CGAGTGCCCA 
AACTGCAAGA AGCGATTTTC TCATTCTGGT TCCTACAGTT CCCACATAAG CAGTAAGAAA 
TGTATCGGTG TGGCGCCTGT GAATGGACGC GTGCGCTCAG GGCTGAAGAC TCCCCAGTGC 
TCCTCCCCGT CCCTTTCTGC TTCTCCGGGA AGTCCAGCTC GACCACAGAT CCGCCAGAAG 
ATAGAGAACA AACCTTTGCA AGAGCAGATT CCAGTTAACC AAATCAAAAC CGAACCTGTG 
GATTATGAAA TCAAGCCTAT CGTGGTGACA TCAGGAATCA ACTGCTCAGC CCCTTTGCAA 
AATGGGGTGT TTAGTAGTGG TAGCCGTTTG CAGGCAACTA ATTCTCCTCA GGGTGTTGTA 
CAAGCTGTTG TTCTGCCAAC TATGGGTCTG GTGTCCCCCA TAAGTATTAA CCTTAATGAC 
ATTCAGAATG TTCTTAAGGT GGCAGTTGAT GGGAATGTAA TAAGACAAGT ATTAGAAAAT 
AACCATGTCA GTCTTGCATC CAAAGAACAA GGGACTCTCA CCTCCGCCAC AATCCAACAA 
GCCGGCCATT CTGTTATTTC CGCTATTAGT CTTCCATTGG TTGATCAAGA CGGTACAACC 
AAGATCATCA TCAATTACAG CTTAGAGCAA CCTAGTCAGC TTCCCCTCGT TGCGCAAAAT 
TTGAAAAAAG AAGCCACAGC TCCTGCAGAA GTCTGCAAAA CGGAGAAGTT GCCAGAAGAT 
CTCACTATCA AAACTGAAAA GGATACTAAG TTTACAGAAG AAAGCACTTG TTTACTTGGT 
GCCGATCACC CAGGAGACAA AAATGCACTT CAAGAACCCA AGCACTATGA TCAGAAAAAG 
AACAGCCTAC TTCCAGAGAG TAGCAAAGCC GAACCTATCA AATCCGAGCC AACTTCTGCC 
GACCCCGAAG ATGGCAATCT TACTCCAAGC CAACCACCTT TAAAGAATCT CTTATCTCTT 
CTGAAAGCAT ACTATGCTTT AAATGCTCAG CCGAGCTCCG AGGAACTTTC TAAAATTGCA 
GATTCAGTAA ACCTACCACT CGACGTGGTA AAAGACTGGT TTGAAAAAAT GCAGAATGGA 
GAAATATCTG TGGAGTCATC TCGGCCCTCA TCTCCAAAAC CTGGACAAGA CAGTGTTACA 
GAAAACAGTG TGGAATCTAA AGATGAAAGT GGATTGCATA ACGGCACGAA CGTTTCACTG 
AAATCCCCCA AACAAGAACC TGAGGAACCG TTAGCTGTTA ATGGTCCAGA AAGTGGCACA 
GCTTCTCCTA CACCACTAAA CCTCTCTTCT TTAAAAAACT CAGAAGGTGC AGCCTGCTCC 
GATGGAACCC CAGATGGGGA GCAGCAGGCC GAGCCTCTTG ATCTATCCTT ACCAAAGCCA 
CATGGAAATG TGCCGGAGCG ACCTTCCATC ACTAGTGTTT ACCAGAGCAG TTCATATTCT 
GTTCAAGAAG AACCCTTGAA CTTGTCTTGT GTTAAAAAAG AGCCAATGCA AGTGGACGAT 
GTTACAAAAG CAGAGCCAAT GCTAATTATT AACCCAAGTG CCAATCCAAT AAATATTGCT 
ATTCCTACAG TCACTGCCCA GTTACCTACA ATCGTTGCCA TTGCCGATCA AAACGGTGTT 
CCGTGTTTAA GAGCTCTAGC TGCCAGTAAG CGAACTATCT TGATTCCCCA AGTGACGTAC 
ACTTACTCAA CCACTGCTAA TCCTTCAGTA CCGGAACCGC AACTCAAGAA CATCCAGCCT 
AATGGAAATC AGGATGAGCG GCAGGATACA AGCTCGGAAG GAGTGTCAAA TGTAGAAGAT 
CAGAATGACT CTGATTCCAC CCCACCCAAA AAGAAACTGA AAAAAACAGA GAACGGTCTC 
TATGCCTGTG ACCTCTGTGA CAAAATTTTC CAGAAAAGCA GCTCACTGCT GAGACATAAG 
TATGAACACA CAGGTAAGAG GCCACATGAA TGTGGAATCT GTGCAAAGGC ATTTAAACAC 
AAACACCATT TAATTGAGCA TATGCGCCTA CATTCTGGAG AAAAGCCCTA TCAATGTGAC 
AAATGTGGGA AACGGTTTTC GCACTCCGGA TCCTACTCTC AGCACATGAA CCACCGCTAC 
TCTTACTGCA AGAAGGAAGC CGAGGAACGA GATGGCATGG AGCAGGAAGA ATCCGCTCAG 
GAGATCCTTG GCAACGAGAA CGTGGATTCC CAGGTGTCTC CATCTCAGAT AGACTCTGAT 
GAGCGCGAGA GCATAACCCG GGAGGAAGAC AGTGAGAAGG AAGAGGAGGA TAAGGAAGAA 
GACGATAAGG AAGAGGAGGA TGAGAAAGAG GAGGATAAGG AAGAGGAGGA AAAGGAAGAG 
GAGGATGAGA AAGAGGAGGA TGAGAAAGAT ATGGATGATG GACAGGAAGA AAAGGAACAT 
GTTGGAGAGG GGGAAATGGA AGAGGGAGAA GCAGAACCAT TGGCAGGTAC TTTGAAGGAT 
GAAGAATGTG TAGAGGAAGA CAAATGTTTG GAAGAAGAGG TCACAGAAGA GAATGTATCG 
GAGAAAGCCC TCCCTCAGCC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zeb1 ( NM_001015808.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001015808.1 Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), mRNA.

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CloneID	OXa24476
Clone ID Related Accession (Same CDS sequence)	NM_001015808.1
Accession Version	NM_001015808.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger E-box-binding homeobox 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC090088.1. ##Evidence-Data-START## Transcript exon combination :: BC090088.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGATG GCCCCAGGTG TAAGCGCAGA AAACAGGCGA ACCCCAGACG GAATAACGTT 
ACCAATTACA ACAGTGTGCT CGAAGCAAAT TCTGATTCGG ATGATGAAGA CAAATTGCAC 
ATAGTGGAAG AGGAAAGTGT TACAGACGCA GCAGATGGCG AGGGCAGCGT TCCAGAAGAT 
GACTTGCCAA CAGACCAAAC AGTATTACCA GAAGGCAGCG AGAGCGCAAA GGGGAGCGGC 
AAAACTTGCT GGGAAGATGA AGTTAAAGAT GAAGAATGCG ATTCAGACCT AGAAAATGAA 
CAAAACCAAG ATCCCAACGT TGAAGAATTC CTACAGCAAA GCGACACTGC TGTAATCTAT 
CCGGAAGCGC CAGAGGATGA CCAGAGGCAA GGCACACCGG AGGCGGTTGG TCAAGATGAA 
AATGGAACGC CAGACGCATT CTCCCAGCTC CTCACCTGCC CGTATTGTGA CAGGGGATAC 
AAGCGCTTCA CGTCTTTGAA GGAACATATT AAGTACCGCC ATGAGAAAAA TGAAGACAAT 
TTCAGTTGCT CGATGTGTAG TTATACCTTT GCTTATAGAA CCCAGCTTGA TCGTCACATG 
ACCTCTCATA AATCAGGAAA AGATCAGAGA CATGTGACGC AGTCTGGAGG CAACCGTAAA 
TTCAAATGCC CTGAGTGTGG AAAAGCTTTT AAATACAAAC ACCATCTGAA AGAACACTTA 
CGGATTCACA GTGGAGAGAA GCCATACGAG TGCCCAAACT GCAAGAAGCG ATTTTCTCAT 
TCTGGTTCCT ACAGTTCCCA CATAAGCAGT AAGAAATGTA TCGGTGTGGC GCCTGTGAAT 
GGACGCGTGC GCTCAGGGCT GAAGACTCCC CAGTGCTCCT CCCCGTCCCT TTCTGCTTCT 
CCGGGAAGTC CAGCTCGACC ACAGATCCGC CAGAAGATAG AGAACAAACC TTTGCAAGAG 
CAGATTCCAG TTAACCAAAT CAAAACCGAA CCTGTGGATT ATGAAATCAA GCCTATCGTG 
GTGACATCAG GAATCAACTG CTCAGCCCCT TTGCAAAATG GGGTGTTTAG TAGTGGTAGC 
CGTTTGCAGG CAACTAATTC TCCTCAGGGT GTTGTACAAG CTGTTGTTCT GCCAACTATG 
GGTCTGGTGT CCCCCATAAG TATTAACCTT AATGACATTC AGAATGTTCT TAAGGTGGCA 
GTTGATGGGA ATGTAATAAG ACAAGTATTA GAAAATAACC ATGTCAGTCT TGCATCCAAA 
GAACAAGGGA CTCTCACCTC CGCCACAATC CAACAAGCCG GCCATTCTGT TATTTCCGCT 
ATTAGTCTTC CATTGGTTGA TCAAGACGGT ACAACCAAGA TCATCATCAA TTACAGCTTA 
GAGCAACCTA GTCAGCTTCC CCTCGTTGCG CAAAATTTGA AAAAAGAAGC CACAGCTCCT 
GCAGAAGTCT GCAAAACGGA GAAGTTGCCA GAAGATCTCA CTATCAAAAC TGAAAAGGAT 
ACTAAGTTTA CAGAAGAAAG CACTTGTTTA CTTGGTGCCG ATCACCCAGG AGACAAAAAT 
GCACTTCAAG AACCCAAGCA CTATGATCAG AAAAAGAACA GCCTACTTCC AGAGAGTAGC 
AAAGCCGAAC CTATCAAATC CGAGCCAACT TCTGCCGACC CCGAAGATGG CAATCTTACT 
CCAAGCCAAC CACCTTTAAA GAATCTCTTA TCTCTTCTGA AAGCATACTA TGCTTTAAAT 
GCTCAGCCGA GCTCCGAGGA ACTTTCTAAA ATTGCAGATT CAGTAAACCT ACCACTCGAC 
GTGGTAAAAG ACTGGTTTGA AAAAATGCAG AGTGGAGAAA TATCTGTGGA GTCATCTCGG 
CCCTCATCTC CAAAACCTGG ACAAGACAGT GTTACAGAAA ACAGTGTGGA ATCTAAAGAT 
GAAAGTGGAT TGCATAACGG CACGAACGTT TCACTGAAAT CCCCCAAACA AGAACCTGAG 
GAACCGTTAG CTGTTAATGG TCCAGAAAGT GGCACAGCTT CTCCTACACC ACTAAACCTC 
TCTTCTTTAA AAAACTCAGA AGGTGCAGCC TGCTCCGATG GAACCCCAGA TGGGGAGCAG 
CAGGCCGAGC CTCTTGATCT ATCCTTACCA AAGCCACATG GAAATGTGCC GGAGCGACCT 
TCCATCACTA GTGTTTACCA GAGCAGTTCA TATTCTGTTC AAGAAGAACC CTTGAACTTG 
TCTTGTGTTA AAAAAGAGCC AATGCAAGTG GACGATGTTA CAAAAGCAGA GCCAATGCTA 
ATTATTAACC CAAGTGCCAA TCCAATAAAT ATTGCTATTC CTACAGTCAC TGCCCAGTTA 
CCTACAATCG TTGCCATTGC CGATCAAAAC GGTGTTCCGT GTTTAAGAGC TCTAGCTGCC 
AGTAAGCGAA CTATCTTGAT TCCCCAAGTG ACGTACACTT ACTCAACCAC TGCTAATCCT 
TCAGTACCGG AACCGCAACT CAAGAACATC CAGCCTAATG GAAATCAGGA TGAGCGGCAG 
GATACAAGCT CGGAAGGAGT GTCAAATGTA GAAGATCAGA ATGACTCTGA TTCCACCCCA 
CCCAAAAAGA AACTGAAAAA AACAGAGAAC GGTCTCTATG CCTGTGACCT CTGTGACAAA 
ATTTTCCAGA AAAGCAGCTC ACTGCTGAGA CATAAGTATG AACACACAGG TAAGAGGCCA 
CATGAATGTG GAATCTGTGC AAAGGCATTT AAACACAAAC ACCATTTAAT TGAGCATATG 
CGCCTACATT CTGGAGAAAA GCCCTATCAA TGTGACAAAT GTGGGAAACG GTTTTCGCAC 
TCCGGATCCT ACTCTCAGCA CATGAACCAC CGCTACTCTT ACTGCAAGAA GGAAGCCGAG 
GAACGAGATG GCATGGAGCA GGAAGAATCC GCTCAGGAGA TCCTTGGCAA CGAGAACGTG 
GATTCCCAGG TGTCTCCATC TCAGATAGAC TCTGATGAGC GCGAGAGCAT AACCCGGGAG 
GAAGACAGTG AGAAGGAAGA GGAGGATAAG GAAGAAGACG ATAAGGAAGA GGAGGATGAG 
AAAGAGGAGG ATAAGGAAGA GGAGGAAAAG GAAGAGGAGG ATGAGAAAGA GGAGGATGAG 
AAAGATATGG ATGATGGACA GGAAGAAAAG GAACATGTTG GAGAGGGGGA AATGGAAGAG 
GGAGAAGCAG AACCATTGGC AGGTACTTTG AAGGATGAAG AATGTGTAGA GGAAGACAAA 
TGTTTGGAAG AAGAGGTCAC AGAAGAGAAT GTATCGGAGA AAGCCCTCCC TCAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001015808.1
CDS	17..3316
Translation	MADGPRCKRRKQANPRRNNVTNYNSVLEANSDSDDEDKLHIVEEESVTDAADGEGSVPEDDLPTDQTVLPEGSESAKGSGKTCWEDEVKDEECDSDLENEQNQDPNVEEFLQQSDTAVIYPEAPEDDQRQGTPEAVGQDENGTPDAFSQLLTCPYCDRGYKRFTSLKEHIKYRHEKNEDNFSCSMCSYTFAYRTQLDRHMTSHKSGKDQRHVTQSGGNRKFKCPECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGVAPVNGRVRSGLKTPQCSSPSLSASPGSPARPQIRQKIENKPLQEQIPVNQIKTEPVDYEIKPIVVTSGINCSAPLQNGVFSSGSRLQATNSPQGVVQAVVLPTMGLVSPISINLNDIQNVLKVAVDGNVIRQVLENNHVSLASKEQGTLTSATIQQAGHSVISAISLPLVDQDGTTKIIINYSLEQPSQLPLVAQNLKKEATAPAEVCKTEKLPEDLTIKTEKDTKFTEESTCLLGADHPGDKNALQEPKHYDQKKNSLLPESSKAEPIKSEPTSADPEDGNLTPSQPPLKNLLSLLKAYYALNAQPSSEELSKIADSVNLPLDVVKDWFEKMQSGEISVESSRPSSPKPGQDSVTENSVESKDESGLHNGTNVSLKSPKQEPEEPLAVNGPESGTASPTPLNLSSLKNSEGAACSDGTPDGEQQAEPLDLSLPKPHGNVPERPSITSVYQSSSYSVQEEPLNLSCVKKEPMQVDDVTKAEPMLIINPSANPINIAIPTVTAQLPTIVAIADQNGVPCLRALAASKRTILIPQVTYTYSTTANPSVPEPQLKNIQPNGNQDERQDTSSEGVSNVEDQNDSDSTPPKKKLKKTENGLYACDLCDKIFQKSSSLLRHKYEHTGKRPHECGICAKAFKHKHHLIEHMRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKKEAEERDGMEQEESAQEILGNENVDSQVSPSQIDSDERESITREEDSEKEEEDKEEDDKEEEDEKEEDKEEEEKEEEDEKEEDEKDMDDGQEEKEHVGEGEMEEGEAEPLAGTLKDEECVEEDKCLEEEVTEENVSEKALPQP

Target ORF information:

RefSeq Version	NM_001015808.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001015808.1

ORF Insert Sequence:

ATGGCGGATG GCCCCAGGTG TAAGCGCAGA AAACAGGCGA ACCCCAGACG GAATAACGTT 
ACCAATTACA ACAGTGTGCT CGAAGCAAAT TCTGATTCGG ATGATGAAGA CAAATTGCAC 
ATAGTGGAAG AGGAAAGTGT TACAGACGCA GCAGATGGCG AGGGCAGCGT TCCAGAAGAT 
GACTTGCCAA CAGACCAAAC AGTATTACCA GAAGGCAGCG AGAGCGCAAA GGGGAGCGGC 
AAAACTTGCT GGGAAGATGA AGTTAAAGAT GAAGAATGCG ATTCAGACCT AGAAAATGAA 
CAAAACCAAG ATCCCAACGT TGAAGAATTC CTACAGCAAA GCGACACTGC TGTAATCTAT 
CCGGAAGCGC CAGAGGATGA CCAGAGGCAA GGCACACCGG AGGCGGTTGG TCAAGATGAA 
AATGGAACGC CAGACGCATT CTCCCAGCTC CTCACCTGCC CGTATTGTGA CAGGGGATAC 
AAGCGCTTCA CGTCTTTGAA GGAACATATT AAGTACCGCC ATGAGAAAAA TGAAGACAAT 
TTCAGTTGCT CGATGTGTAG TTATACCTTT GCTTATAGAA CCCAGCTTGA TCGTCACATG 
ACCTCTCATA AATCAGGAAA AGATCAGAGA CATGTGACGC AGTCTGGAGG CAACCGTAAA 
TTCAAATGCC CTGAGTGTGG AAAAGCTTTT AAATACAAAC ACCATCTGAA AGAACACTTA 
CGGATTCACA GTGGAGAGAA GCCATACGAG TGCCCAAACT GCAAGAAGCG ATTTTCTCAT 
TCTGGTTCCT ACAGTTCCCA CATAAGCAGT AAGAAATGTA TCGGTGTGGC GCCTGTGAAT 
GGACGCGTGC GCTCAGGGCT GAAGACTCCC CAGTGCTCCT CCCCGTCCCT TTCTGCTTCT 
CCGGGAAGTC CAGCTCGACC ACAGATCCGC CAGAAGATAG AGAACAAACC TTTGCAAGAG 
CAGATTCCAG TTAACCAAAT CAAAACCGAA CCTGTGGATT ATGAAATCAA GCCTATCGTG 
GTGACATCAG GAATCAACTG CTCAGCCCCT TTGCAAAATG GGGTGTTTAG TAGTGGTAGC 
CGTTTGCAGG CAACTAATTC TCCTCAGGGT GTTGTACAAG CTGTTGTTCT GCCAACTATG 
GGTCTGGTGT CCCCCATAAG TATTAACCTT AATGACATTC AGAATGTTCT TAAGGTGGCA 
GTTGATGGGA ATGTAATAAG ACAAGTATTA GAAAATAACC ATGTCAGTCT TGCATCCAAA 
GAACAAGGGA CTCTCACCTC CGCCACAATC CAACAAGCCG GCCATTCTGT TATTTCCGCT 
ATTAGTCTTC CATTGGTTGA TCAAGACGGT ACAACCAAGA TCATCATCAA TTACAGCTTA 
GAGCAACCTA GTCAGCTTCC CCTCGTTGCG CAAAATTTGA AAAAAGAAGC CACAGCTCCT 
GCAGAAGTCT GCAAAACGGA GAAGTTGCCA GAAGATCTCA CTATCAAAAC TGAAAAGGAT 
ACTAAGTTTA CAGAAGAAAG CACTTGTTTA CTTGGTGCCG ATCACCCAGG AGACAAAAAT 
GCACTTCAAG AACCCAAGCA CTATGATCAG AAAAAGAACA GCCTACTTCC AGAGAGTAGC 
AAAGCCGAAC CTATCAAATC CGAGCCAACT TCTGCCGACC CCGAAGATGG CAATCTTACT 
CCAAGCCAAC CACCTTTAAA GAATCTCTTA TCTCTTCTGA AAGCATACTA TGCTTTAAAT 
GCTCAGCCGA GCTCCGAGGA ACTTTCTAAA ATTGCAGATT CAGTAAACCT ACCACTCGAC 
GTGGTAAAAG ACTGGTTTGA AAAAATGCAG AGTGGAGAAA TATCTGTGGA GTCATCTCGG 
CCCTCATCTC CAAAACCTGG ACAAGACAGT GTTACAGAAA ACAGTGTGGA ATCTAAAGAT 
GAAAGTGGAT TGCATAACGG CACGAACGTT TCACTGAAAT CCCCCAAACA AGAACCTGAG 
GAACCGTTAG CTGTTAATGG TCCAGAAAGT GGCACAGCTT CTCCTACACC ACTAAACCTC 
TCTTCTTTAA AAAACTCAGA AGGTGCAGCC TGCTCCGATG GAACCCCAGA TGGGGAGCAG 
CAGGCCGAGC CTCTTGATCT ATCCTTACCA AAGCCACATG GAAATGTGCC GGAGCGACCT 
TCCATCACTA GTGTTTACCA GAGCAGTTCA TATTCTGTTC AAGAAGAACC CTTGAACTTG 
TCTTGTGTTA AAAAAGAGCC AATGCAAGTG GACGATGTTA CAAAAGCAGA GCCAATGCTA 
ATTATTAACC CAAGTGCCAA TCCAATAAAT ATTGCTATTC CTACAGTCAC TGCCCAGTTA 
CCTACAATCG TTGCCATTGC CGATCAAAAC GGTGTTCCGT GTTTAAGAGC TCTAGCTGCC 
AGTAAGCGAA CTATCTTGAT TCCCCAAGTG ACGTACACTT ACTCAACCAC TGCTAATCCT 
TCAGTACCGG AACCGCAACT CAAGAACATC CAGCCTAATG GAAATCAGGA TGAGCGGCAG 
GATACAAGCT CGGAAGGAGT GTCAAATGTA GAAGATCAGA ATGACTCTGA TTCCACCCCA 
CCCAAAAAGA AACTGAAAAA AACAGAGAAC GGTCTCTATG CCTGTGACCT CTGTGACAAA 
ATTTTCCAGA AAAGCAGCTC ACTGCTGAGA CATAAGTATG AACACACAGG TAAGAGGCCA 
CATGAATGTG GAATCTGTGC AAAGGCATTT AAACACAAAC ACCATTTAAT TGAGCATATG 
CGCCTACATT CTGGAGAAAA GCCCTATCAA TGTGACAAAT GTGGGAAACG GTTTTCGCAC 
TCCGGATCCT ACTCTCAGCA CATGAACCAC CGCTACTCTT ACTGCAAGAA GGAAGCCGAG 
GAACGAGATG GCATGGAGCA GGAAGAATCC GCTCAGGAGA TCCTTGGCAA CGAGAACGTG 
GATTCCCAGG TGTCTCCATC TCAGATAGAC TCTGATGAGC GCGAGAGCAT AACCCGGGAG 
GAAGACAGTG AGAAGGAAGA GGAGGATAAG GAAGAAGACG ATAAGGAAGA GGAGGATGAG 
AAAGAGGAGG ATAAGGAAGA GGAGGAAAAG GAAGAGGAGG ATGAGAAAGA GGAGGATGAG 
AAAGATATGG ATGATGGACA GGAAGAAAAG GAACATGTTG GAGAGGGGGA AATGGAAGAG 
GGAGAAGCAG AACCATTGGC AGGTACTTTG AAGGATGAAG AATGTGTAGA GGAAGACAAA 
TGTTTGGAAG AAGAGGTCAC AGAAGAGAAT GTATCGGAGA AAGCCCTCCC TCAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zeb1 ( XM_012964650.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820104.1 Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), transcript variant X1, mRNA.

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CloneID	OXa27416
Clone ID Related Accession (Same CDS sequence)	XM_012964650.2
Accession Version	XM_012964650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger E-box-binding homeobox 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964650.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG GCCCCAGGTG TAAGCGCAGA AAACAGGCGA ACCCCAGACG GAATAACGTT 
ACCAATTACA ACAGTGTGCT CGAAGCAAAT TCTGATTCGG ATGATGAAGA CAAATTGCAC 
ATAGTGGAAG AGGAAAGTGT TACAGACGCA GCAGATGGCG AGGGCAGCGT TCCAGAAGAT 
GACTTGCCAA CAGACCAAAC AGTATTACCA GAAGGCAGCG AGAGCGCAAA GGGGAGCGGC 
AAAACTTGCT GGGAAGATGA AGAAGGGGCA GATTGCAGTG ATGAGATCCT GGGGCCTGAA 
GCCGAAGCTG ATGAACTTGG ATGTACAGTT AAAGATGAAG AATGCGATTC AGACCTAGAA 
AATGAACAAA ACCAAGATCC CAACGTTGAA GAATTCCTAC AGCAAAGCGA CACTGCTGTA 
ATCTATCCGG AAGCGCCAGA GGATGACCAG AGGCAAGGCA CACCGGAGGC GGTTGGTCAA 
GATGAAAATG GAACGCCAGA CGCATTCTCC CAGCTCCTCA CCTGCCCGTA TTGTGACAGG 
GGATACAAGC GCTTCACGTC TTTGAAGGAA CATATTAAGT ACCGCCATGA GAAAAATGAA 
GACAATTTCA GTTGCTCGAT GTGTAGTTAT ACCTTTGCTT ATAGAACCCA GCTTGATCGT 
CACATGACCT CTCATAAATC AGGAAAAGAT CAGAGACATG TGACGCAGTC TGGAGGCAAC 
CGTAAATTCA AATGCCCTGA GTGTGGAAAA GCTTTTAAAT ACAAACACCA TCTGAAAGAA 
CACTTACGGA TTCACAGTGG AGAGAAGCCA TACGAGTGCC CAAACTGCAA GAAGCGATTT 
TCTCATTCTG GTTCCTACAG TTCCCACATA AGCAGTAAGA AATGTATCGG TGTGGCGCCT 
GTGAATGGAC GCGTGCGCTC AGGGCTGAAG ACTCCCCAGT GCTCCTCCCC GTCCCTTTCT 
GCTTCTCCGG GAAGTCCAGC TCGACCACAG ATCCGCCAGA AGATAGAGAA CAAACCTTTG 
CAAGAGCAGA TTCCAGTTAA CCAAATCAAA ACCGAACCTG TGGATTATGA AATCAAGCCT 
ATCGTGGTGA CATCAGGAAT CAACTGCTCA GCCCCTTTGC AAAATGGGGT GTTTAGTAGT 
GGTAGCCGTT TGCAGGCAAC TAATTCTCCT CAGGGTGTTG TACAAGCTGT TGTTCTGCCA 
ACTATGGGTC TGGTGTCCCC CATAAGTATT AACCTTAATG ACATTCAGAA TGTTCTTAAG 
GTGGCAGTTG ATGGGAATGT AATAAGACAA GTATTAGAAA ATAACCATGT CAGTCTTGCA 
TCCAAAGAAC AAGGGACTCT CACCTCCGCC ACAATCCAAC AAGCCGGCCA TTCTGTTATT 
TCCGCTATTA GTCTTCCATT GGTTGATCAA GACGGTACAA CCAAGATCAT CATCAATTAC 
AGCTTAGAGC AACCTAGTCA GCTTCCCCTC GTTGCGCAAA ATTTGAAAAA AGAAGCCACA 
GCTCCTGCAG AAGTCTGCAA AACGGAGAAG TTGCCAGAAG ATCTCACTAT CAAAACTGAA 
AAGGATACTA AGTTTACAGA AGAAAGCACT TGTTTACTTG GTGCCGATCA CCCAGGAGAC 
AAAAATGCAC TTCAAGAACC CAAGCACTAT GATCAGAAAA AGAACAGCCT ACTTCCAGAG 
AGTAGCAAAG CCGAACCTAT CAAATCCGAG CCAACTTCTG CCGACCCCGA AGATGGCAAT 
CTTACTCCAA GCCAACCACC TTTAAAGAAT CTCTTATCTC TTCTGAAAGC ATACTATGCT 
TTAAATGCTC AGCCGAGCTC CGAGGAACTT TCTAAAATTG CAGATTCAGT AAACCTACCA 
CTCGACGTGG TAAAAGACTG GTTTGAAAAA ATGCAGAATG GAGAAATATC TGTGGAGTCA 
TCTCGGCCCT CATCTCCAAA ACCTGGACAA GACAGTGTTA CAGAAAACAG TGTGGAATCT 
AAAGATGAAA GTGGATTGCA TAACGGCACG AACGTTTCAC TGAAATCCCC CAAACAAGAA 
CCTGAGGAAC CGTTAGCTGT TAATGGTCCA GAAAGTGGCA CAGCTTCTCC TACACCACTA 
AACCTCTCTT CTTTAAAAAA CTCAGAAGGT GCAGCCTGCT CCGATGGAAC CCCAGATGGG 
GAGCAGCAGG CCGAGCCTCT TGATCTATCC TTACCAAAGC CACATGGAAA TGTGCCGGAG 
CGACCTTCCA TCACTAGTGT TTACCAGAGC AGTTCATATT CTGTTCAAGA AGAACCCTTG 
AACTTGTCTT GTGTTAAAAA AGAGCCAATG CAAGTGGACG ATGTTACAAA AGCAGAGCCA 
ATGCTAATTA TTAACCCAAG TGCCAATCCA ATAAATATTG CTATTCCTAC AGTCACTGCC 
CAGTTACCTA CAATCGTTGC CATTGCCGAT CAAAACGGTG TTCCGTGTTT AAGAGCTCTA 
GCTGCCAGTA AGCGAACTAT CTTGATTCCC CAAGTGACGT ACACTTACTC AACCACTGCT 
AATCCTTCAG TACCGGAACC GCAACTCAAG AACATCCAGC CTAATGGAAA TCAGGATGAG 
CGGCAGGATA CAAGCTCGGA AGGAGTGTCA AATGTAGAAG ATCAGAATGA CTCTGATTCC 
ACCCCACCCA AAAAGAAACT GAAAAAAACA GAGAACGGTC TCTATGCCTG TGACCTCTGT 
GACAAAATTT TCCAGAAAAG CAGCTCACTG CTGAGACATA AGTATGAACA CACAGGTAAG 
AGGCCACATG AATGTGGAAT CTGTGCAAAG GCATTTAAAC ACAAACACCA TTTAATTGAG 
CATATGCGCC TACATTCTGG AGAAAAGCCC TATCAATGTG ACAAATGTGG GAAACGGTTT 
TCGCACTCCG GATCCTACTC TCAGCACATG AACCACCGCT ACTCTTACTG CAAGAAGGAA 
GCCGAGGAAC GAGATGGCAT GGAGCAGGAA GAATCCGCTC AGGAGATCCT TGGCAACGAG 
AACGTGGATT CCCAGGTGTC TCCATCTCAG ATAGACTCTG ATGAGCGCGA GAGCATAACC 
CGGGAGGAAG ACAGTGAGAA GGAAGAGGAG GATAAGGAAG AAGACGATAA GGAAGAGGAG 
GATGAGAAAG AGGAGGATAA GGAAGAGGAG GAAAAGGAAG AGGAGGATGA GAAAGAGGAG 
GATGAGAAAG ATATGGATGA TGGACAGGAA GAAAAGGAAC ATGTTGGAGA GGGGGAAATG 
GAAGAGGGAG AAGCAGAACC ATTGGCAGGT ACTTTGAAGG ATGAAGAATG TGTAGAGGAA 
GACAAATGTT TGGAAGAAGA GGTCACAGAA GAGAATGTAT CGGAGAAAGC CCTCCCTCAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820104.1
CDS	48..3413
Translation	MADGPRCKRRKQANPRRNNVTNYNSVLEANSDSDDEDKLHIVEEESVTDAADGEGSVPEDDLPTDQTVLPEGSESAKGSGKTCWEDEEGADCSDEILGPEAEADELGCTVKDEECDSDLENEQNQDPNVEEFLQQSDTAVIYPEAPEDDQRQGTPEAVGQDENGTPDAFSQLLTCPYCDRGYKRFTSLKEHIKYRHEKNEDNFSCSMCSYTFAYRTQLDRHMTSHKSGKDQRHVTQSGGNRKFKCPECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGVAPVNGRVRSGLKTPQCSSPSLSASPGSPARPQIRQKIENKPLQEQIPVNQIKTEPVDYEIKPIVVTSGINCSAPLQNGVFSSGSRLQATNSPQGVVQAVVLPTMGLVSPISINLNDIQNVLKVAVDGNVIRQVLENNHVSLASKEQGTLTSATIQQAGHSVISAISLPLVDQDGTTKIIINYSLEQPSQLPLVAQNLKKEATAPAEVCKTEKLPEDLTIKTEKDTKFTEESTCLLGADHPGDKNALQEPKHYDQKKNSLLPESSKAEPIKSEPTSADPEDGNLTPSQPPLKNLLSLLKAYYALNAQPSSEELSKIADSVNLPLDVVKDWFEKMQNGEISVESSRPSSPKPGQDSVTENSVESKDESGLHNGTNVSLKSPKQEPEEPLAVNGPESGTASPTPLNLSSLKNSEGAACSDGTPDGEQQAEPLDLSLPKPHGNVPERPSITSVYQSSSYSVQEEPLNLSCVKKEPMQVDDVTKAEPMLIINPSANPINIAIPTVTAQLPTIVAIADQNGVPCLRALAASKRTILIPQVTYTYSTTANPSVPEPQLKNIQPNGNQDERQDTSSEGVSNVEDQNDSDSTPPKKKLKKTENGLYACDLCDKIFQKSSSLLRHKYEHTGKRPHECGICAKAFKHKHHLIEHMRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKKEAEERDGMEQEESAQEILGNENVDSQVSPSQIDSDERESITREEDSEKEEEDKEEDDKEEEDEKEEDKEEEEKEEEDEKEEDEKDMDDGQEEKEHVGEGEMEEGEAEPLAGTLKDEECVEEDKCLEEEVTEENVSEKALPQP

Target ORF information:

RefSeq Version	XM_012964650.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964650.2

ORF Insert Sequence:

ATGGCGGATG GCCCCAGGTG TAAGCGCAGA AAACAGGCGA ACCCCAGACG GAATAACGTT 
ACCAATTACA ACAGTGTGCT CGAAGCAAAT TCTGATTCGG ATGATGAAGA CAAATTGCAC 
ATAGTGGAAG AGGAAAGTGT TACAGACGCA GCAGATGGCG AGGGCAGCGT TCCAGAAGAT 
GACTTGCCAA CAGACCAAAC AGTATTACCA GAAGGCAGCG AGAGCGCAAA GGGGAGCGGC 
AAAACTTGCT GGGAAGATGA AGAAGGGGCA GATTGCAGTG ATGAGATCCT GGGGCCTGAA 
GCCGAAGCTG ATGAACTTGG ATGTACAGTT AAAGATGAAG AATGCGATTC AGACCTAGAA 
AATGAACAAA ACCAAGATCC CAACGTTGAA GAATTCCTAC AGCAAAGCGA CACTGCTGTA 
ATCTATCCGG AAGCGCCAGA GGATGACCAG AGGCAAGGCA CACCGGAGGC GGTTGGTCAA 
GATGAAAATG GAACGCCAGA CGCATTCTCC CAGCTCCTCA CCTGCCCGTA TTGTGACAGG 
GGATACAAGC GCTTCACGTC TTTGAAGGAA CATATTAAGT ACCGCCATGA GAAAAATGAA 
GACAATTTCA GTTGCTCGAT GTGTAGTTAT ACCTTTGCTT ATAGAACCCA GCTTGATCGT 
CACATGACCT CTCATAAATC AGGAAAAGAT CAGAGACATG TGACGCAGTC TGGAGGCAAC 
CGTAAATTCA AATGCCCTGA GTGTGGAAAA GCTTTTAAAT ACAAACACCA TCTGAAAGAA 
CACTTACGGA TTCACAGTGG AGAGAAGCCA TACGAGTGCC CAAACTGCAA GAAGCGATTT 
TCTCATTCTG GTTCCTACAG TTCCCACATA AGCAGTAAGA AATGTATCGG TGTGGCGCCT 
GTGAATGGAC GCGTGCGCTC AGGGCTGAAG ACTCCCCAGT GCTCCTCCCC GTCCCTTTCT 
GCTTCTCCGG GAAGTCCAGC TCGACCACAG ATCCGCCAGA AGATAGAGAA CAAACCTTTG 
CAAGAGCAGA TTCCAGTTAA CCAAATCAAA ACCGAACCTG TGGATTATGA AATCAAGCCT 
ATCGTGGTGA CATCAGGAAT CAACTGCTCA GCCCCTTTGC AAAATGGGGT GTTTAGTAGT 
GGTAGCCGTT TGCAGGCAAC TAATTCTCCT CAGGGTGTTG TACAAGCTGT TGTTCTGCCA 
ACTATGGGTC TGGTGTCCCC CATAAGTATT AACCTTAATG ACATTCAGAA TGTTCTTAAG 
GTGGCAGTTG ATGGGAATGT AATAAGACAA GTATTAGAAA ATAACCATGT CAGTCTTGCA 
TCCAAAGAAC AAGGGACTCT CACCTCCGCC ACAATCCAAC AAGCCGGCCA TTCTGTTATT 
TCCGCTATTA GTCTTCCATT GGTTGATCAA GACGGTACAA CCAAGATCAT CATCAATTAC 
AGCTTAGAGC AACCTAGTCA GCTTCCCCTC GTTGCGCAAA ATTTGAAAAA AGAAGCCACA 
GCTCCTGCAG AAGTCTGCAA AACGGAGAAG TTGCCAGAAG ATCTCACTAT CAAAACTGAA 
AAGGATACTA AGTTTACAGA AGAAAGCACT TGTTTACTTG GTGCCGATCA CCCAGGAGAC 
AAAAATGCAC TTCAAGAACC CAAGCACTAT GATCAGAAAA AGAACAGCCT ACTTCCAGAG 
AGTAGCAAAG CCGAACCTAT CAAATCCGAG CCAACTTCTG CCGACCCCGA AGATGGCAAT 
CTTACTCCAA GCCAACCACC TTTAAAGAAT CTCTTATCTC TTCTGAAAGC ATACTATGCT 
TTAAATGCTC AGCCGAGCTC CGAGGAACTT TCTAAAATTG CAGATTCAGT AAACCTACCA 
CTCGACGTGG TAAAAGACTG GTTTGAAAAA ATGCAGAATG GAGAAATATC TGTGGAGTCA 
TCTCGGCCCT CATCTCCAAA ACCTGGACAA GACAGTGTTA CAGAAAACAG TGTGGAATCT 
AAAGATGAAA GTGGATTGCA TAACGGCACG AACGTTTCAC TGAAATCCCC CAAACAAGAA 
CCTGAGGAAC CGTTAGCTGT TAATGGTCCA GAAAGTGGCA CAGCTTCTCC TACACCACTA 
AACCTCTCTT CTTTAAAAAA CTCAGAAGGT GCAGCCTGCT CCGATGGAAC CCCAGATGGG 
GAGCAGCAGG CCGAGCCTCT TGATCTATCC TTACCAAAGC CACATGGAAA TGTGCCGGAG 
CGACCTTCCA TCACTAGTGT TTACCAGAGC AGTTCATATT CTGTTCAAGA AGAACCCTTG 
AACTTGTCTT GTGTTAAAAA AGAGCCAATG CAAGTGGACG ATGTTACAAA AGCAGAGCCA 
ATGCTAATTA TTAACCCAAG TGCCAATCCA ATAAATATTG CTATTCCTAC AGTCACTGCC 
CAGTTACCTA CAATCGTTGC CATTGCCGAT CAAAACGGTG TTCCGTGTTT AAGAGCTCTA 
GCTGCCAGTA AGCGAACTAT CTTGATTCCC CAAGTGACGT ACACTTACTC AACCACTGCT 
AATCCTTCAG TACCGGAACC GCAACTCAAG AACATCCAGC CTAATGGAAA TCAGGATGAG 
CGGCAGGATA CAAGCTCGGA AGGAGTGTCA AATGTAGAAG ATCAGAATGA CTCTGATTCC 
ACCCCACCCA AAAAGAAACT GAAAAAAACA GAGAACGGTC TCTATGCCTG TGACCTCTGT 
GACAAAATTT TCCAGAAAAG CAGCTCACTG CTGAGACATA AGTATGAACA CACAGGTAAG 
AGGCCACATG AATGTGGAAT CTGTGCAAAG GCATTTAAAC ACAAACACCA TTTAATTGAG 
CATATGCGCC TACATTCTGG AGAAAAGCCC TATCAATGTG ACAAATGTGG GAAACGGTTT 
TCGCACTCCG GATCCTACTC TCAGCACATG AACCACCGCT ACTCTTACTG CAAGAAGGAA 
GCCGAGGAAC GAGATGGCAT GGAGCAGGAA GAATCCGCTC AGGAGATCCT TGGCAACGAG 
AACGTGGATT CCCAGGTGTC TCCATCTCAG ATAGACTCTG ATGAGCGCGA GAGCATAACC 
CGGGAGGAAG ACAGTGAGAA GGAAGAGGAG GATAAGGAAG AAGACGATAA GGAAGAGGAG 
GATGAGAAAG AGGAGGATAA GGAAGAGGAG GAAAAGGAAG AGGAGGATGA GAAAGAGGAG 
GATGAGAAAG ATATGGATGA TGGACAGGAA GAAAAGGAAC ATGTTGGAGA GGGGGAAATG 
GAAGAGGGAG AAGCAGAACC ATTGGCAGGT ACTTTGAAGG ATGAAGAATG TGTAGAGGAA 
GACAAATGTT TGGAAGAAGA GGTCACAGAA GAGAATGTAT CGGAGAAAGC CCTCCCTCAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zeb1 ( XM_012964653.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820107.1 Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), transcript variant X3, mRNA.

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CloneID	OXa27419
Clone ID Related Accession (Same CDS sequence)	XM_012964653.3 , XM_012964653.2 , XM_031903368.1
Accession Version	XM_012964653.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger E-box-binding homeobox 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964653.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTAGTT ATACCTTTGC TTATAGAACC CAGCTTGATC GTCACATGAC CTCTCATAAA 
TCAGGAAAAG ATCAGAGACA TGTGACGCAG TCTGGAGGCA ACCGTAAATT CAAATGCCCT 
GAGTGTGGAA AAGCTTTTAA ATACAAACAC CATCTGAAAG AACACTTACG GATTCACAGT 
GGAGAGAAGC CATACGAGTG CCCAAACTGC AAGAAGCGAT TTTCTCATTC TGGTTCCTAC 
AGTTCCCACA TAAGCAGTAA GAAATGTATC GGTGTGGCGC CTGTGAATGG ACGCGTGCGC 
TCAGGGCTGA AGACTCCCCA GTGCTCCTCC CCGTCCCTTT CTGCTTCTCC GGGAAGTCCA 
GCTCGACCAC AGATCCGCCA GAAGATAGAG AACAAACCTT TGCAAGAGCA GATTCCAGTT 
AACCAAATCA AAACCGAACC TGTGGATTAT GAAATCAAGC CTATCGTGGT GACATCAGGA 
ATCAACTGCT CAGCCCCTTT GCAAAATGGG GTGTTTAGTA GTGGTAGCCG TTTGCAGGCA 
ACTAATTCTC CTCAGGGTGT TGTACAAGCT GTTGTTCTGC CAACTATGGG TCTGGTGTCC 
CCCATAAGTA TTAACCTTAA TGACATTCAG AATGTTCTTA AGGTGGCAGT TGATGGGAAT 
GTAATAAGAC AAGTATTAGA AAATAACCAT GTCAGTCTTG CATCCAAAGA ACAAGGGACT 
CTCACCTCCG CCACAATCCA ACAAGCCGGC CATTCTGTTA TTTCCGCTAT TAGTCTTCCA 
TTGGTTGATC AAGACGGTAC AACCAAGATC ATCATCAATT ACAGCTTAGA GCAACCTAGT 
CAGCTTCCCC TCGTTGCGCA AAATTTGAAA AAAGAAGCCA CAGCTCCTGC AGAAGTCTGC 
AAAACGGAGA AGTTGCCAGA AGATCTCACT ATCAAAACTG AAAAGGATAC TAAGTTTACA 
GAAGAAAGCA CTTGTTTACT TGGTGCCGAT CACCCAGGAG ACAAAAATGC ACTTCAAGAA 
CCCAAGCACT ATGATCAGAA AAAGAACAGC CTACTTCCAG AGAGTAGCAA AGCCGAACCT 
ATCAAATCCG AGCCAACTTC TGCCGACCCC GAAGATGGCA ATCTTACTCC AAGCCAACCA 
CCTTTAAAGA ATCTCTTATC TCTTCTGAAA GCATACTATG CTTTAAATGC TCAGCCGAGC 
TCCGAGGAAC TTTCTAAAAT TGCAGATTCA GTAAACCTAC CACTCGACGT GGTAAAAGAC 
TGGTTTGAAA AAATGCAGAA TGGAGAAATA TCTGTGGAGT CATCTCGGCC CTCATCTCCA 
AAACCTGGAC AAGACAGTGT TACAGAAAAC AGTGTGGAAT CTAAAGATGA AAGTGGATTG 
CATAACGGCA CGAACGTTTC ACTGAAATCC CCCAAACAAG AACCTGAGGA ACCGTTAGCT 
GTTAATGGTC CAGAAAGTGG CACAGCTTCT CCTACACCAC TAAACCTCTC TTCTTTAAAA 
AACTCAGAAG GTGCAGCCTG CTCCGATGGA ACCCCAGATG GGGAGCAGCA GGCCGAGCCT 
CTTGATCTAT CCTTACCAAA GCCACATGGA AATGTGCCGG AGCGACCTTC CATCACTAGT 
GTTTACCAGA GCAGTTCATA TTCTGTTCAA GAAGAACCCT TGAACTTGTC TTGTGTTAAA 
AAAGAGCCAA TGCAAGTGGA CGATGTTACA AAAGCAGAGC CAATGCTAAT TATTAACCCA 
AGTGCCAATC CAATAAATAT TGCTATTCCT ACAGTCACTG CCCAGTTACC TACAATCGTT 
GCCATTGCCG ATCAAAACGG TGTTCCGTGT TTAAGAGCTC TAGCTGCCAG TAAGCGAACT 
ATCTTGATTC CCCAAGTGAC GTACACTTAC TCAACCACTG CTAATCCTTC AGTACCGGAA 
CCGCAACTCA AGAACATCCA GCCTAATGGA AATCAGGATG AGCGGCAGGA TACAAGCTCG 
GAAGGAGTGT CAAATGTAGA AGATCAGAAT GACTCTGATT CCACCCCACC CAAAAAGAAA 
CTGAAAAAAA CAGAGAACGG TCTCTATGCC TGTGACCTCT GTGACAAAAT TTTCCAGAAA 
AGCAGCTCAC TGCTGAGACA TAAGTATGAA CACACAGGTA AGAGGCCACA TGAATGTGGA 
ATCTGTGCAA AGGCATTTAA ACACAAACAC CATTTAATTG AGCATATGCG CCTACATTCT 
GGAGAAAAGC CCTATCAATG TGACAAATGT GGGAAACGGT TTTCGCACTC CGGATCCTAC 
TCTCAGCACA TGAACCACCG CTACTCTTAC TGCAAGAAGG AAGCCGAGGA ACGAGATGGC 
ATGGAGCAGG AAGAATCCGC TCAGGAGATC CTTGGCAACG AGAACGTGGA TTCCCAGGTG 
TCTCCATCTC AGATAGACTC TGATGAGCGC GAGAGCATAA CCCGGGAGGA AGACAGTGAG 
AAGGAAGAGG AGGATAAGGA AGAAGACGAT AAGGAAGAGG AGGATGAGAA AGAGGAGGAT 
AAGGAAGAGG AGGAAAAGGA AGAGGAGGAT GAGAAAGAGG AGGATGAGAA AGATATGGAT 
GATGGACAGG AAGAAAAGGA ACATGTTGGA GAGGGGGAAA TGGAAGAGGG AGAAGCAGAA 
CCATTGGCAG GTACTTTGAA GGATGAAGAA TGTGTAGAGG AAGACAAATG TTTGGAAGAA 
GAGGTCACAG AAGAGAATGT ATCGGAGAAA GCCCTCCCTC AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820107.1
CDS	938..3685
Translation	MCSYTFAYRTQLDRHMTSHKSGKDQRHVTQSGGNRKFKCPECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGVAPVNGRVRSGLKTPQCSSPSLSASPGSPARPQIRQKIENKPLQEQIPVNQIKTEPVDYEIKPIVVTSGINCSAPLQNGVFSSGSRLQATNSPQGVVQAVVLPTMGLVSPISINLNDIQNVLKVAVDGNVIRQVLENNHVSLASKEQGTLTSATIQQAGHSVISAISLPLVDQDGTTKIIINYSLEQPSQLPLVAQNLKKEATAPAEVCKTEKLPEDLTIKTEKDTKFTEESTCLLGADHPGDKNALQEPKHYDQKKNSLLPESSKAEPIKSEPTSADPEDGNLTPSQPPLKNLLSLLKAYYALNAQPSSEELSKIADSVNLPLDVVKDWFEKMQNGEISVESSRPSSPKPGQDSVTENSVESKDESGLHNGTNVSLKSPKQEPEEPLAVNGPESGTASPTPLNLSSLKNSEGAACSDGTPDGEQQAEPLDLSLPKPHGNVPERPSITSVYQSSSYSVQEEPLNLSCVKKEPMQVDDVTKAEPMLIINPSANPINIAIPTVTAQLPTIVAIADQNGVPCLRALAASKRTILIPQVTYTYSTTANPSVPEPQLKNIQPNGNQDERQDTSSEGVSNVEDQNDSDSTPPKKKLKKTENGLYACDLCDKIFQKSSSLLRHKYEHTGKRPHECGICAKAFKHKHHLIEHMRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKKEAEERDGMEQEESAQEILGNENVDSQVSPSQIDSDERESITREEDSEKEEEDKEEDDKEEEDEKEEDKEEEEKEEEDEKEEDEKDMDDGQEEKEHVGEGEMEEGEAEPLAGTLKDEECVEEDKCLEEEVTEENVSEKALPQP

Target ORF information:

RefSeq Version	XM_012964653.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger E-box binding homeobox 1 (zeb1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964653.2

ORF Insert Sequence:

ATGTGTAGTT ATACCTTTGC TTATAGAACC CAGCTTGATC GTCACATGAC CTCTCATAAA 
TCAGGAAAAG ATCAGAGACA TGTGACGCAG TCTGGAGGCA ACCGTAAATT CAAATGCCCT 
GAGTGTGGAA AAGCTTTTAA ATACAAACAC CATCTGAAAG AACACTTACG GATTCACAGT 
GGAGAGAAGC CATACGAGTG CCCAAACTGC AAGAAGCGAT TTTCTCATTC TGGTTCCTAC 
AGTTCCCACA TAAGCAGTAA GAAATGTATC GGTGTGGCGC CTGTGAATGG ACGCGTGCGC 
TCAGGGCTGA AGACTCCCCA GTGCTCCTCC CCGTCCCTTT CTGCTTCTCC GGGAAGTCCA 
GCTCGACCAC AGATCCGCCA GAAGATAGAG AACAAACCTT TGCAAGAGCA GATTCCAGTT 
AACCAAATCA AAACCGAACC TGTGGATTAT GAAATCAAGC CTATCGTGGT GACATCAGGA 
ATCAACTGCT CAGCCCCTTT GCAAAATGGG GTGTTTAGTA GTGGTAGCCG TTTGCAGGCA 
ACTAATTCTC CTCAGGGTGT TGTACAAGCT GTTGTTCTGC CAACTATGGG TCTGGTGTCC 
CCCATAAGTA TTAACCTTAA TGACATTCAG AATGTTCTTA AGGTGGCAGT TGATGGGAAT 
GTAATAAGAC AAGTATTAGA AAATAACCAT GTCAGTCTTG CATCCAAAGA ACAAGGGACT 
CTCACCTCCG CCACAATCCA ACAAGCCGGC CATTCTGTTA TTTCCGCTAT TAGTCTTCCA 
TTGGTTGATC AAGACGGTAC AACCAAGATC ATCATCAATT ACAGCTTAGA GCAACCTAGT 
CAGCTTCCCC TCGTTGCGCA AAATTTGAAA AAAGAAGCCA CAGCTCCTGC AGAAGTCTGC 
AAAACGGAGA AGTTGCCAGA AGATCTCACT ATCAAAACTG AAAAGGATAC TAAGTTTACA 
GAAGAAAGCA CTTGTTTACT TGGTGCCGAT CACCCAGGAG ACAAAAATGC ACTTCAAGAA 
CCCAAGCACT ATGATCAGAA AAAGAACAGC CTACTTCCAG AGAGTAGCAA AGCCGAACCT 
ATCAAATCCG AGCCAACTTC TGCCGACCCC GAAGATGGCA ATCTTACTCC AAGCCAACCA 
CCTTTAAAGA ATCTCTTATC TCTTCTGAAA GCATACTATG CTTTAAATGC TCAGCCGAGC 
TCCGAGGAAC TTTCTAAAAT TGCAGATTCA GTAAACCTAC CACTCGACGT GGTAAAAGAC 
TGGTTTGAAA AAATGCAGAA TGGAGAAATA TCTGTGGAGT CATCTCGGCC CTCATCTCCA 
AAACCTGGAC AAGACAGTGT TACAGAAAAC AGTGTGGAAT CTAAAGATGA AAGTGGATTG 
CATAACGGCA CGAACGTTTC ACTGAAATCC CCCAAACAAG AACCTGAGGA ACCGTTAGCT 
GTTAATGGTC CAGAAAGTGG CACAGCTTCT CCTACACCAC TAAACCTCTC TTCTTTAAAA 
AACTCAGAAG GTGCAGCCTG CTCCGATGGA ACCCCAGATG GGGAGCAGCA GGCCGAGCCT 
CTTGATCTAT CCTTACCAAA GCCACATGGA AATGTGCCGG AGCGACCTTC CATCACTAGT 
GTTTACCAGA GCAGTTCATA TTCTGTTCAA GAAGAACCCT TGAACTTGTC TTGTGTTAAA 
AAAGAGCCAA TGCAAGTGGA CGATGTTACA AAAGCAGAGC CAATGCTAAT TATTAACCCA 
AGTGCCAATC CAATAAATAT TGCTATTCCT ACAGTCACTG CCCAGTTACC TACAATCGTT 
GCCATTGCCG ATCAAAACGG TGTTCCGTGT TTAAGAGCTC TAGCTGCCAG TAAGCGAACT 
ATCTTGATTC CCCAAGTGAC GTACACTTAC TCAACCACTG CTAATCCTTC AGTACCGGAA 
CCGCAACTCA AGAACATCCA GCCTAATGGA AATCAGGATG AGCGGCAGGA TACAAGCTCG 
GAAGGAGTGT CAAATGTAGA AGATCAGAAT GACTCTGATT CCACCCCACC CAAAAAGAAA 
CTGAAAAAAA CAGAGAACGG TCTCTATGCC TGTGACCTCT GTGACAAAAT TTTCCAGAAA 
AGCAGCTCAC TGCTGAGACA TAAGTATGAA CACACAGGTA AGAGGCCACA TGAATGTGGA 
ATCTGTGCAA AGGCATTTAA ACACAAACAC CATTTAATTG AGCATATGCG CCTACATTCT 
GGAGAAAAGC CCTATCAATG TGACAAATGT GGGAAACGGT TTTCGCACTC CGGATCCTAC 
TCTCAGCACA TGAACCACCG CTACTCTTAC TGCAAGAAGG AAGCCGAGGA ACGAGATGGC 
ATGGAGCAGG AAGAATCCGC TCAGGAGATC CTTGGCAACG AGAACGTGGA TTCCCAGGTG 
TCTCCATCTC AGATAGACTC TGATGAGCGC GAGAGCATAA CCCGGGAGGA AGACAGTGAG 
AAGGAAGAGG AGGATAAGGA AGAAGACGAT AAGGAAGAGG AGGATGAGAA AGAGGAGGAT 
AAGGAAGAGG AGGAAAAGGA AGAGGAGGAT GAGAAAGAGG AGGATGAGAA AGATATGGAT 
GATGGACAGG AAGAAAAGGA ACATGTTGGA GAGGGGGAAA TGGAAGAGGG AGAAGCAGAA 
CCATTGGCAG GTACTTTGAA GGATGAAGAA TGTGTAGAGG AAGACAAATG TTTGGAAGAA 
GAGGTCACAG AAGAGAATGT ATCGGAGAAA GCCCTCCCTC AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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zeb1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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