llgl2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	llgl2
Entrez Gene ID	548396
Full Name	LLGL2, scribble cell polarity complex component
Synonyms	Lgl-2,hgl,lgl2
General protein information	Preferred Names LLGL2, scribble cell polarity complex component Names lethal(2) giant larvae protein homolog 2 lethal giant larva 2 lethal giant larvae 2 lethal giant larvae homolog 2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012971730.2	XP_012827184.1	lethal(2) giant larvae protein homolog 2 isoform X1
XM_012971729.3	XP_012827183.1	LLGL scribble cell polarity complex component 2 isoform X1
XM_031894071.1	XP_031749931.1	LLGL scribble cell polarity complex component 2 isoform X1
NM_001015691.1	NP_001015691.1	LLGL scribble cell polarity complex component 2
XM_012971729.2	XP_012827183.1	lethal(2) giant larvae protein homolog 2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	LLGL2	NP_001026973.1
Bos taurus (cattle)	LLGL2	NP_001092568.1
Mus musculus (house mouse)	Llgl2	NP_663413.2
Danio rerio (zebrafish)	llgl2	NP_997747.1
Pan troglodytes (chimpanzee)	LLGL2	XP_523719.3
Canis lupus familiaris (dog)	LLGL2	XP_005624156.1
Gallus gallus (chicken)	LLGL2	XP_420130.3
Xenopus tropicalis (tropical clawed frog)	llgl2	NP_001015691.1
Rattus norvegicus (Norway rat)	Llgl2	NP_001121021.1

Gene Ontology
Bibliography

Related articles in PubMed

aPKC, Crumbs3 and Lgl2 control apicobasal polarity in early vertebrate development.
Chalmers AD, Pambos M, Mason J, Lang S, Wylie C, Papalopulu N
Development (Cambridge, England)132(5)977-86(2005 Mar)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following llgl2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the llgl2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa27348	XM_031894071.1 Latest version!	Xenopus tropicalis LLGL2, scribble cell polarity complex component (llgl2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa27348	XM_012971729.3 Latest version!	Xenopus tropicalis LLGL2, scribble cell polarity complex component (llgl2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa22108	NM_001015691.1 Latest version!	Xenopus tropicalis LLGL2, scribble cell polarity complex component (llgl2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa27348	XM_012971730.2 Latest version!	Xenopus tropicalis lethal giant larvae homolog 2 (llgl2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OXa27348	XM_012971729.2	Xenopus tropicalis lethal giant larvae homolog 2 (llgl2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

llgl2 ( XM_012971729.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827183.1 Xenopus tropicalis LLGL2, scribble cell polarity complex component (llgl2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa27348
Clone ID Related Accession (Same CDS sequence)	XM_012971730.2 , XM_012971729.3 , XM_031894071.1 , XM_012971729.2
Accession Version	XM_012971729.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LLGL scribble cell polarity complex component 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971729.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGCT TCCTTCGCTC CGGCCATGAT TCTGTGAGGG AGCGGCTGAA ACGGGACCTT 
TTCCAGTTTA ACAAGACAGT GGAACATGGA TTCCCCCACC AGCCCAGCGC TTTGGGGTAC 
AGTGACTGCC TTAGCCTCAT GGCGATTGGA ACTCGTTCCG GAGCCCTCAA AGTATATGGC 
TCCCCAGGGG TGGAATTTAT GGGTCTCCAT GATGAGAATA ACTCTGTGAC AAAGATCAGC 
TTCATCCCAG ACCAGTGCCA ACTCGTCACC GTCCTCGATG ACAACAGCCT CCACCTGTGG 
TCATTTCCGC ATCACGACGG GGTCTCAGAA CTGCAGGAAG AGAAGAAATT CACCCTACGT 
GGCCCCCCTG GGTCTCCTCC GAGCGTGACG CAGATCAAGG TGCTGTTGCC GCACTCCTCG 
GGGGAGAAGC TGTACGTGGG CACCGAGGGG GGCAGTGTGT TCATTCTCGA GCTGCCATCT 
CTGCGGGTGC TAGAAGAGAA GACAGTGGGG CATGAAGCGG CTCTTCAAGG TCTGCCTGAC 
GAGTACAGCA GCAGGAGAGC GTTTGAGTCT GTGGAGGCCT TGCAGGAGAA TCCCCGCAAT 
CCAGACCAGC TTTTGATTGG TTACAGCCGC GGAATGATGG TGACGTGGGA CTTGGTAAAG 
GGACGCAGCG TGCAATATTT TCTGGGCAAA CAGCAACTAG AGAACGTTTT CTGGTGCCGC 
GATGGGAAGC AGATAGTCAG CTGTCACAGC GATGGGGGCT ACAACCAATG GGAGGTCTCA 
AATGAGCTAG TACAGGTTTC TCCCAAGGAG AGCAAAGTGC CATACGGTCC CTTCCGCTGC 
AAGGCAATCA GTAAAATAAC CTGGCACACC ACGCGCAGAA GCCGGTCTCC GTACGTGGTG 
TTCCAAGGAG GGATGCCCAT GGCCAGCTAT GGGGACAGGC ACATCATATC CGTGGTACAT 
GGGAACCAGC AGACTGCTTT TGACTTTACC TCTCGGATCA TTGACCACGT AGTTATTGCC 
AACCCGGATC CCTCAGCAGA GTACGATGAC CCGCGGGCCC TGCTGGTGCT GGTAGAAGAG 
GAGTTGGTGA TGATCGACCT GCAGAGCCCC GGCTGGCCAG TGATCCAGTG CCCCTACCTC 
ATCTCCCTCC ATTGTTCAGC CATTACCTGC TCCCATCACA TCTCCTCCAT CCCCTTGAAG 
CTATGGGAGC GGATAATCAG CGCGGGGAGG AAGCAGAACA CTCAGTACTC TAACATGCCG 
TGGCCCATTG ATGGTGGCGT CAATTTGGCT CCTGCGCCCC CTCAGAGGGA CCTGCTTCTT 
ACTGGACATG AGGATGGCAC TGTGCGCTTC TGGGATGCCT CCGGCATTTG CCTTCAGCTA 
ATGTACAAGA TGAGCACAGT GAGTGTCTTC CAGACCGAGT CGGACCACAA CGACAATGTC 
AACTCCAATG AGGAGGAAGA GTGGCCTCCA CTGAGGAAAG TTGGCTCCTT TGACCCTTAC 
AGTGATGACC CCAGGCTGGG CATACAGAAG ATTTACATGT GCAAATACAG TGGTTACCTC 
TCTGTGGCTG GCACGGCTGG ACAGGTTCTG GTTATGGAGC TGAATGATGA AGCTGCAGAG 
CATGTGGTGG ACGAGACCTT AGTAGACCTC CTTCAGGAAC AAGAAGGCTA CCGCTGGAAG 
GGCCACGAAC GGCTCCATGC TCGGGAGGAC CCTGTCCGCT TTCCACCGGG GTTCCAGCCA 
TTTGCCCTGG TGCAGTGCCA GCCCCCTGCT GCTGTTACTT CCCTGGCCCT GCACTCTGAG 
TGGCGGCTGT TGGCCTTTGG TACCAGCCAT GGCTTCGGCT TGTACGACTT TCAGCAGAAG 
CGCCAAGTAA TGGCCAAGTG CACGTTACAT GCCAGTGACC AAATGGCGTT GGAGGGTCCC 
CTGTCACGGG TTAAATCTCT GAAGAAATCT TTGCGTCAGT CATTCCGCCG CATTAGGAGG 
AGTCAAGTCA ACAAGAGAAG ACCAGGGCCT GATAGGGTGC AAGAGGCCAA TGCTCGATTT 
GAGCAGGAAG CTCTGCAAGA GATGGAACTG GCCCCCGTGC AGAGGCGGAT AGAGGCCCGC 
TCGGCTGAGG ATTCCTTCAC CGGATTCGTG CGCACATTGT ACTTTGCCGA CACCTACCTG 
AGGGACAGCT CTAGGCACAC GCCTTCCCTC TGGGCGGGGA CAAATGGCGG CACAGTGTAT 
GCGTTCTCTT TGCGGGTGCC ACCCACAGAG CGCCGAATGG AAGAGGCAGT GGCGGCAACG 
CAGGCTAAGG AAATCCAACT CATGCACCGG GCTCCGGTTG TGGGGATCCT GGTTCTGGAC 
GGTCACAGCG TTCCTCTCCC AGAACCCTTA GAGGCAGCTC ACGATCTCTC CAAGAGCCCC 
GACATGCAAG GCAGCCACCA GCTATTGGTC ACTTCCGAGG AGCAGTTTAA GGTCTTTACT 
CTGCCCAAAG TGAGCAGCAA ACTGAAGCTA AAACTGACGG CCACGGACGG CTGCCGCGTG 
CGCAAGATGA CGGTGTGCAC CTACGGTAGC TGCAAGTCGG AGGAGCACAG CGAGAACCAC 
CTGACCATTC TTACCAACCT GGGGGATATT CAGGTGGTGT CACTGCCCCA TCTGAAGCCA 
CAAGTCCGGT ACGACTGCAT CCGCAAGGAA GATGTGAGCG GCATCGCCTC CTGTGTCTTC 
ACCAAACACG GGCAGGGGTT TTATCTAATC TCCCCCTCTG AGTTGGAACG GTTTTCGCTA 
TCCACATGCT GGTTGGTGGC TCCCCGTGCC AGAGTGGATC CTCCGGAGCC CCCTCGTAAC 
TCGCGGCTAC GGCGGGGGGG CAGCACTGAC CAACCAACTG ACAGGTCCAG GTCTTCTGGG 
ACGGACCACA CAGGAGGCCA GCGGGCCCAG GGGACACTGA TGGAACATGC GCTGCTAAAT 
GACGAGAAGA TGCTGAAGGA AATCCAGAGC ACGCTGGAAG GAACCCGAAT AAGCTACAGC 
GAAAGGAGTT CCCGGCAGAT GAGCGTTGGA CACAGACTGA TCAATGGGGC AGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827183.1
CDS	57..3116
Translation	MKRFLRSGHDSVRERLKRDLFQFNKTVEHGFPHQPSALGYSDCLSLMAIGTRSGALKVYGSPGVEFMGLHDENNSVTKISFIPDQCQLVTVLDDNSLHLWSFPHHDGVSELQEEKKFTLRGPPGSPPSVTQIKVLLPHSSGEKLYVGTEGGSVFILELPSLRVLEEKTVGHEAALQGLPDEYSSRRAFESVEALQENPRNPDQLLIGYSRGMMVTWDLVKGRSVQYFLGKQQLENVFWCRDGKQIVSCHSDGGYNQWEVSNELVQVSPKESKVPYGPFRCKAISKITWHTTRRSRSPYVVFQGGMPMASYGDRHIISVVHGNQQTAFDFTSRIIDHVVIANPDPSAEYDDPRALLVLVEEELVMIDLQSPGWPVIQCPYLISLHCSAITCSHHISSIPLKLWERIISAGRKQNTQYSNMPWPIDGGVNLAPAPPQRDLLLTGHEDGTVRFWDASGICLQLMYKMSTVSVFQTESDHNDNVNSNEEEEWPPLRKVGSFDPYSDDPRLGIQKIYMCKYSGYLSVAGTAGQVLVMELNDEAAEHVVDETLVDLLQEQEGYRWKGHERLHAREDPVRFPPGFQPFALVQCQPPAAVTSLALHSEWRLLAFGTSHGFGLYDFQQKRQVMAKCTLHASDQMALEGPLSRVKSLKKSLRQSFRRIRRSQVNKRRPGPDRVQEANARFEQEALQEMELAPVQRRIEARSAEDSFTGFVRTLYFADTYLRDSSRHTPSLWAGTNGGTVYAFSLRVPPTERRMEEAVAATQAKEIQLMHRAPVVGILVLDGHSVPLPEPLEAAHDLSKSPDMQGSHQLLVTSEEQFKVFTLPKVSSKLKLKLTATDGCRVRKMTVCTYGSCKSEEHSENHLTILTNLGDIQVVSLPHLKPQVRYDCIRKEDVSGIASCVFTKHGQGFYLISPSELERFSLSTCWLVAPRARVDPPEPPRNSRLRRGGSTDQPTDRSRSSGTDHTGGQRAQGTLMEHALLNDEKMLKEIQSTLEGTRISYSERSSRQMSVGHRLINGAAE

Target ORF information:

RefSeq Version	XM_012971729.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis LLGL2, scribble cell polarity complex component (llgl2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971729.3

ORF Insert Sequence:

ATGAAGCGCT TCCTTCGCTC CGGCCATGAT TCTGTGAGGG AGCGGCTGAA ACGGGACCTT 
TTCCAGTTTA ACAAGACAGT GGAACATGGA TTCCCCCACC AGCCCAGCGC TTTGGGGTAC 
AGTGACTGCC TTAGCCTCAT GGCGATTGGA ACTCGTTCCG GAGCCCTCAA AGTATATGGC 
TCCCCAGGGG TGGAATTTAT GGGTCTCCAT GATGAGAATA ACTCTGTGAC AAAGATCAGC 
TTCATCCCAG ACCAGTGCCA ACTCGTCACC GTCCTCGATG ACAACAGCCT CCACCTGTGG 
TCATTTCCGC ATCACGACGG GGTCTCAGAA CTGCAGGAAG AGAAGAAATT CACCCTACGT 
GGCCCCCCTG GGTCTCCTCC GAGCGTGACG CAGATCAAGG TGCTGTTGCC GCACTCCTCG 
GGGGAGAAGC TGTACGTGGG CACCGAGGGG GGCAGTGTGT TCATTCTCGA GCTGCCATCT 
CTGCGGGTGC TAGAAGAGAA GACAGTGGGG CATGAAGCGG CTCTTCAAGG TCTGCCTGAC 
GAGTACAGCA GCAGGAGAGC GTTTGAGTCT GTGGAGGCCT TGCAGGAGAA TCCCCGCAAT 
CCAGACCAGC TTTTGATTGG TTACAGCCGC GGAATGATGG TGACGTGGGA CTTGGTAAAG 
GGACGCAGCG TGCAATATTT TCTGGGCAAA CAGCAACTAG AGAACGTTTT CTGGTGCCGC 
GATGGGAAGC AGATAGTCAG CTGTCACAGC GATGGGGGCT ACAACCAATG GGAGGTCTCA 
AATGAGCTAG TACAGGTTTC TCCCAAGGAG AGCAAAGTGC CATACGGTCC CTTCCGCTGC 
AAGGCAATCA GTAAAATAAC CTGGCACACC ACGCGCAGAA GCCGGTCTCC GTACGTGGTG 
TTCCAAGGAG GGATGCCCAT GGCCAGCTAT GGGGACAGGC ACATCATATC CGTGGTACAT 
GGGAACCAGC AGACTGCTTT TGACTTTACC TCTCGGATCA TTGACCACGT AGTTATTGCC 
AACCCGGATC CCTCAGCAGA GTACGATGAC CCGCGGGCCC TGCTGGTGCT GGTAGAAGAG 
GAGTTGGTGA TGATCGACCT GCAGAGCCCC GGCTGGCCAG TGATCCAGTG CCCCTACCTC 
ATCTCCCTCC ATTGTTCAGC CATTACCTGC TCCCATCACA TCTCCTCCAT CCCCTTGAAG 
CTATGGGAGC GGATAATCAG CGCGGGGAGG AAGCAGAACA CTCAGTACTC TAACATGCCG 
TGGCCCATTG ATGGTGGCGT CAATTTGGCT CCTGCGCCCC CTCAGAGGGA CCTGCTTCTT 
ACTGGACATG AGGATGGCAC TGTGCGCTTC TGGGATGCCT CCGGCATTTG CCTTCAGCTA 
ATGTACAAGA TGAGCACAGT GAGTGTCTTC CAGACCGAGT CGGACCACAA CGACAATGTC 
AACTCCAATG AGGAGGAAGA GTGGCCTCCA CTGAGGAAAG TTGGCTCCTT TGACCCTTAC 
AGTGATGACC CCAGGCTGGG CATACAGAAG ATTTACATGT GCAAATACAG TGGTTACCTC 
TCTGTGGCTG GCACGGCTGG ACAGGTTCTG GTTATGGAGC TGAATGATGA AGCTGCAGAG 
CATGTGGTGG ACGAGACCTT AGTAGACCTC CTTCAGGAAC AAGAAGGCTA CCGCTGGAAG 
GGCCACGAAC GGCTCCATGC TCGGGAGGAC CCTGTCCGCT TTCCACCGGG GTTCCAGCCA 
TTTGCCCTGG TGCAGTGCCA GCCCCCTGCT GCTGTTACTT CCCTGGCCCT GCACTCTGAG 
TGGCGGCTGT TGGCCTTTGG TACCAGCCAT GGCTTCGGCT TGTACGACTT TCAGCAGAAG 
CGCCAAGTAA TGGCCAAGTG CACGTTACAT GCCAGTGACC AAATGGCGTT GGAGGGTCCC 
CTGTCACGGG TTAAATCTCT GAAGAAATCT TTGCGTCAGT CATTCCGCCG CATTAGGAGG 
AGTCAAGTCA ACAAGAGAAG ACCAGGGCCT GATAGGGTGC AAGAGGCCAA TGCTCGATTT 
GAGCAGGAAG CTCTGCAAGA GATGGAACTG GCCCCCGTGC AGAGGCGGAT AGAGGCCCGC 
TCGGCTGAGG ATTCCTTCAC CGGATTCGTG CGCACATTGT ACTTTGCCGA CACCTACCTG 
AGGGACAGCT CTAGGCACAC GCCTTCCCTC TGGGCGGGGA CAAATGGCGG CACAGTGTAT 
GCGTTCTCTT TGCGGGTGCC ACCCACAGAG CGCCGAATGG AAGAGGCAGT GGCGGCAACG 
CAGGCTAAGG AAATCCAACT CATGCACCGG GCTCCGGTTG TGGGGATCCT GGTTCTGGAC 
GGTCACAGCG TTCCTCTCCC AGAACCCTTA GAGGCAGCTC ACGATCTCTC CAAGAGCCCC 
GACATGCAAG GCAGCCACCA GCTATTGGTC ACTTCCGAGG AGCAGTTTAA GGTCTTTACT 
CTGCCCAAAG TGAGCAGCAA ACTGAAGCTA AAACTGACGG CCACGGACGG CTGCCGCGTG 
CGCAAGATGA CGGTGTGCAC CTACGGTAGC TGCAAGTCGG AGGAGCACAG CGAGAACCAC 
CTGACCATTC TTACCAACCT GGGGGATATT CAGGTGGTGT CACTGCCCCA TCTGAAGCCA 
CAAGTCCGGT ACGACTGCAT CCGCAAGGAA GATGTGAGCG GCATCGCCTC CTGTGTCTTC 
ACCAAACACG GGCAGGGGTT TTATCTAATC TCCCCCTCTG AGTTGGAACG GTTTTCGCTA 
TCCACATGCT GGTTGGTGGC TCCCCGTGCC AGAGTGGATC CTCCGGAGCC CCCTCGTAAC 
TCGCGGCTAC GGCGGGGGGG CAGCACTGAC CAACCAACTG ACAGGTCCAG GTCTTCTGGG 
ACGGACCACA CAGGAGGCCA GCGGGCCCAG GGGACACTGA TGGAACATGC GCTGCTAAAT 
GACGAGAAGA TGCTGAAGGA AATCCAGAGC ACGCTGGAAG GAACCCGAAT AAGCTACAGC 
GAAAGGAGTT CCCGGCAGAT GAGCGTTGGA CACAGACTGA TCAATGGGGC AGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

llgl2 ( XM_031894071.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031749931.1 Xenopus tropicalis LLGL2, scribble cell polarity complex component (llgl2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa27348
Clone ID Related Accession (Same CDS sequence)	XM_012971730.2 , XM_012971729.3 , XM_031894071.1 , XM_012971729.2
Accession Version	XM_031894071.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LLGL scribble cell polarity complex component 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGCT TCCTTCGCTC CGGCCATGAT TCTGTGAGGG AGCGGCTGAA ACGGGACCTT 
TTCCAGTTTA ACAAGACAGT GGAACATGGA TTCCCCCACC AGCCCAGCGC TTTGGGGTAC 
AGTGACTGCC TTAGCCTCAT GGCGATTGGA ACTCGTTCCG GAGCCCTCAA AGTATATGGC 
TCCCCAGGGG TGGAATTTAT GGGTCTCCAT GATGAGAATA ACTCTGTGAC AAAGATCAGC 
TTCATCCCAG ACCAGTGCCA ACTCGTCACC GTCCTCGATG ACAACAGCCT CCACCTGTGG 
TCATTTCCGC ATCACGACGG GGTCTCAGAA CTGCAGGAAG AGAAGAAATT CACCCTACGT 
GGCCCCCCTG GGTCTCCTCC GAGCGTGACG CAGATCAAGG TGCTGTTGCC GCACTCCTCG 
GGGGAGAAGC TGTACGTGGG CACCGAGGGG GGCAGTGTGT TCATTCTCGA GCTGCCATCT 
CTGCGGGTGC TAGAAGAGAA GACAGTGGGG CATGAAGCGG CTCTTCAAGG TCTGCCTGAC 
GAGTACAGCA GCAGGAGAGC GTTTGAGTCT GTGGAGGCCT TGCAGGAGAA TCCCCGCAAT 
CCAGACCAGC TTTTGATTGG TTACAGCCGC GGAATGATGG TGACGTGGGA CTTGGTAAAG 
GGACGCAGCG TGCAATATTT TCTGGGCAAA CAGCAACTAG AGAACGTTTT CTGGTGCCGC 
GATGGGAAGC AGATAGTCAG CTGTCACAGC GATGGGGGCT ACAACCAATG GGAGGTCTCA 
AATGAGCTAG TACAGGTTTC TCCCAAGGAG AGCAAAGTGC CATACGGTCC CTTCCGCTGC 
AAGGCAATCA GTAAAATAAC CTGGCACACC ACGCGCAGAA GCCGGTCTCC GTACGTGGTG 
TTCCAAGGAG GGATGCCCAT GGCCAGCTAT GGGGACAGGC ACATCATATC CGTGGTACAT 
GGGAACCAGC AGACTGCTTT TGACTTTACC TCTCGGATCA TTGACCACGT AGTTATTGCC 
AACCCGGATC CCTCAGCAGA GTACGATGAC CCGCGGGCCC TGCTGGTGCT GGTAGAAGAG 
GAGTTGGTGA TGATCGACCT GCAGAGCCCC GGCTGGCCAG TGATCCAGTG CCCCTACCTC 
ATCTCCCTCC ATTGTTCAGC CATTACCTGC TCCCATCACA TCTCCTCCAT CCCCTTGAAG 
CTATGGGAGC GGATAATCAG CGCGGGGAGG AAGCAGAACA CTCAGTACTC TAACATGCCG 
TGGCCCATTG ATGGTGGCGT CAATTTGGCT CCTGCGCCCC CTCAGAGGGA CCTGCTTCTT 
ACTGGACATG AGGATGGCAC TGTGCGCTTC TGGGATGCCT CCGGCATTTG CCTTCAGCTA 
ATGTACAAGA TGAGCACAGT GAGTGTCTTC CAGACCGAGT CGGACCACAA CGACAATGTC 
AACTCCAATG AGGAGGAAGA GTGGCCTCCA CTGAGGAAAG TTGGCTCCTT TGACCCTTAC 
AGTGATGACC CCAGGCTGGG CATACAGAAG ATTTACATGT GCAAATACAG TGGTTACCTC 
TCTGTGGCTG GCACGGCTGG ACAGGTTCTG GTTATGGAGC TGAATGATGA AGCTGCAGAG 
CATGTGGTGG ACGAGACCTT AGTAGACCTC CTTCAGGAAC AAGAAGGCTA CCGCTGGAAG 
GGCCACGAAC GGCTCCATGC TCGGGAGGAC CCTGTCCGCT TTCCACCGGG GTTCCAGCCA 
TTTGCCCTGG TGCAGTGCCA GCCCCCTGCT GCTGTTACTT CCCTGGCCCT GCACTCTGAG 
TGGCGGCTGT TGGCCTTTGG TACCAGCCAT GGCTTCGGCT TGTACGACTT TCAGCAGAAG 
CGCCAAGTAA TGGCCAAGTG CACGTTACAT GCCAGTGACC AAATGGCGTT GGAGGGTCCC 
CTGTCACGGG TTAAATCTCT GAAGAAATCT TTGCGTCAGT CATTCCGCCG CATTAGGAGG 
AGTCAAGTCA ACAAGAGAAG ACCAGGGCCT GATAGGGTGC AAGAGGCCAA TGCTCGATTT 
GAGCAGGAAG CTCTGCAAGA GATGGAACTG GCCCCCGTGC AGAGGCGGAT AGAGGCCCGC 
TCGGCTGAGG ATTCCTTCAC CGGATTCGTG CGCACATTGT ACTTTGCCGA CACCTACCTG 
AGGGACAGCT CTAGGCACAC GCCTTCCCTC TGGGCGGGGA CAAATGGCGG CACAGTGTAT 
GCGTTCTCTT TGCGGGTGCC ACCCACAGAG CGCCGAATGG AAGAGGCAGT GGCGGCAACG 
CAGGCTAAGG AAATCCAACT CATGCACCGG GCTCCGGTTG TGGGGATCCT GGTTCTGGAC 
GGTCACAGCG TTCCTCTCCC AGAACCCTTA GAGGCAGCTC ACGATCTCTC CAAGAGCCCC 
GACATGCAAG GCAGCCACCA GCTATTGGTC ACTTCCGAGG AGCAGTTTAA GGTCTTTACT 
CTGCCCAAAG TGAGCAGCAA ACTGAAGCTA AAACTGACGG CCACGGACGG CTGCCGCGTG 
CGCAAGATGA CGGTGTGCAC CTACGGTAGC TGCAAGTCGG AGGAGCACAG CGAGAACCAC 
CTGACCATTC TTACCAACCT GGGGGATATT CAGGTGGTGT CACTGCCCCA TCTGAAGCCA 
CAAGTCCGGT ACGACTGCAT CCGCAAGGAA GATGTGAGCG GCATCGCCTC CTGTGTCTTC 
ACCAAACACG GGCAGGGGTT TTATCTAATC TCCCCCTCTG AGTTGGAACG GTTTTCGCTA 
TCCACATGCT GGTTGGTGGC TCCCCGTGCC AGAGTGGATC CTCCGGAGCC CCCTCGTAAC 
TCGCGGCTAC GGCGGGGGGG CAGCACTGAC CAACCAACTG ACAGGTCCAG GTCTTCTGGG 
ACGGACCACA CAGGAGGCCA GCGGGCCCAG GGGACACTGA TGGAACATGC GCTGCTAAAT 
GACGAGAAGA TGCTGAAGGA AATCCAGAGC ACGCTGGAAG GAACCCGAAT AAGCTACAGC 
GAAAGGAGTT CCCGGCAGAT GAGCGTTGGA CACAGACTGA TCAATGGGGC AGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031749931.1
CDS	45..3104
Translation	MKRFLRSGHDSVRERLKRDLFQFNKTVEHGFPHQPSALGYSDCLSLMAIGTRSGALKVYGSPGVEFMGLHDENNSVTKISFIPDQCQLVTVLDDNSLHLWSFPHHDGVSELQEEKKFTLRGPPGSPPSVTQIKVLLPHSSGEKLYVGTEGGSVFILELPSLRVLEEKTVGHEAALQGLPDEYSSRRAFESVEALQENPRNPDQLLIGYSRGMMVTWDLVKGRSVQYFLGKQQLENVFWCRDGKQIVSCHSDGGYNQWEVSNELVQVSPKESKVPYGPFRCKAISKITWHTTRRSRSPYVVFQGGMPMASYGDRHIISVVHGNQQTAFDFTSRIIDHVVIANPDPSAEYDDPRALLVLVEEELVMIDLQSPGWPVIQCPYLISLHCSAITCSHHISSIPLKLWERIISAGRKQNTQYSNMPWPIDGGVNLAPAPPQRDLLLTGHEDGTVRFWDASGICLQLMYKMSTVSVFQTESDHNDNVNSNEEEEWPPLRKVGSFDPYSDDPRLGIQKIYMCKYSGYLSVAGTAGQVLVMELNDEAAEHVVDETLVDLLQEQEGYRWKGHERLHAREDPVRFPPGFQPFALVQCQPPAAVTSLALHSEWRLLAFGTSHGFGLYDFQQKRQVMAKCTLHASDQMALEGPLSRVKSLKKSLRQSFRRIRRSQVNKRRPGPDRVQEANARFEQEALQEMELAPVQRRIEARSAEDSFTGFVRTLYFADTYLRDSSRHTPSLWAGTNGGTVYAFSLRVPPTERRMEEAVAATQAKEIQLMHRAPVVGILVLDGHSVPLPEPLEAAHDLSKSPDMQGSHQLLVTSEEQFKVFTLPKVSSKLKLKLTATDGCRVRKMTVCTYGSCKSEEHSENHLTILTNLGDIQVVSLPHLKPQVRYDCIRKEDVSGIASCVFTKHGQGFYLISPSELERFSLSTCWLVAPRARVDPPEPPRNSRLRRGGSTDQPTDRSRSSGTDHTGGQRAQGTLMEHALLNDEKMLKEIQSTLEGTRISYSERSSRQMSVGHRLINGAAE

Target ORF information:

RefSeq Version	XM_031894071.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis LLGL2, scribble cell polarity complex component (llgl2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894071.1

ORF Insert Sequence:

ATGAAGCGCT TCCTTCGCTC CGGCCATGAT TCTGTGAGGG AGCGGCTGAA ACGGGACCTT 
TTCCAGTTTA ACAAGACAGT GGAACATGGA TTCCCCCACC AGCCCAGCGC TTTGGGGTAC 
AGTGACTGCC TTAGCCTCAT GGCGATTGGA ACTCGTTCCG GAGCCCTCAA AGTATATGGC 
TCCCCAGGGG TGGAATTTAT GGGTCTCCAT GATGAGAATA ACTCTGTGAC AAAGATCAGC 
TTCATCCCAG ACCAGTGCCA ACTCGTCACC GTCCTCGATG ACAACAGCCT CCACCTGTGG 
TCATTTCCGC ATCACGACGG GGTCTCAGAA CTGCAGGAAG AGAAGAAATT CACCCTACGT 
GGCCCCCCTG GGTCTCCTCC GAGCGTGACG CAGATCAAGG TGCTGTTGCC GCACTCCTCG 
GGGGAGAAGC TGTACGTGGG CACCGAGGGG GGCAGTGTGT TCATTCTCGA GCTGCCATCT 
CTGCGGGTGC TAGAAGAGAA GACAGTGGGG CATGAAGCGG CTCTTCAAGG TCTGCCTGAC 
GAGTACAGCA GCAGGAGAGC GTTTGAGTCT GTGGAGGCCT TGCAGGAGAA TCCCCGCAAT 
CCAGACCAGC TTTTGATTGG TTACAGCCGC GGAATGATGG TGACGTGGGA CTTGGTAAAG 
GGACGCAGCG TGCAATATTT TCTGGGCAAA CAGCAACTAG AGAACGTTTT CTGGTGCCGC 
GATGGGAAGC AGATAGTCAG CTGTCACAGC GATGGGGGCT ACAACCAATG GGAGGTCTCA 
AATGAGCTAG TACAGGTTTC TCCCAAGGAG AGCAAAGTGC CATACGGTCC CTTCCGCTGC 
AAGGCAATCA GTAAAATAAC CTGGCACACC ACGCGCAGAA GCCGGTCTCC GTACGTGGTG 
TTCCAAGGAG GGATGCCCAT GGCCAGCTAT GGGGACAGGC ACATCATATC CGTGGTACAT 
GGGAACCAGC AGACTGCTTT TGACTTTACC TCTCGGATCA TTGACCACGT AGTTATTGCC 
AACCCGGATC CCTCAGCAGA GTACGATGAC CCGCGGGCCC TGCTGGTGCT GGTAGAAGAG 
GAGTTGGTGA TGATCGACCT GCAGAGCCCC GGCTGGCCAG TGATCCAGTG CCCCTACCTC 
ATCTCCCTCC ATTGTTCAGC CATTACCTGC TCCCATCACA TCTCCTCCAT CCCCTTGAAG 
CTATGGGAGC GGATAATCAG CGCGGGGAGG AAGCAGAACA CTCAGTACTC TAACATGCCG 
TGGCCCATTG ATGGTGGCGT CAATTTGGCT CCTGCGCCCC CTCAGAGGGA CCTGCTTCTT 
ACTGGACATG AGGATGGCAC TGTGCGCTTC TGGGATGCCT CCGGCATTTG CCTTCAGCTA 
ATGTACAAGA TGAGCACAGT GAGTGTCTTC CAGACCGAGT CGGACCACAA CGACAATGTC 
AACTCCAATG AGGAGGAAGA GTGGCCTCCA CTGAGGAAAG TTGGCTCCTT TGACCCTTAC 
AGTGATGACC CCAGGCTGGG CATACAGAAG ATTTACATGT GCAAATACAG TGGTTACCTC 
TCTGTGGCTG GCACGGCTGG ACAGGTTCTG GTTATGGAGC TGAATGATGA AGCTGCAGAG 
CATGTGGTGG ACGAGACCTT AGTAGACCTC CTTCAGGAAC AAGAAGGCTA CCGCTGGAAG 
GGCCACGAAC GGCTCCATGC TCGGGAGGAC CCTGTCCGCT TTCCACCGGG GTTCCAGCCA 
TTTGCCCTGG TGCAGTGCCA GCCCCCTGCT GCTGTTACTT CCCTGGCCCT GCACTCTGAG 
TGGCGGCTGT TGGCCTTTGG TACCAGCCAT GGCTTCGGCT TGTACGACTT TCAGCAGAAG 
CGCCAAGTAA TGGCCAAGTG CACGTTACAT GCCAGTGACC AAATGGCGTT GGAGGGTCCC 
CTGTCACGGG TTAAATCTCT GAAGAAATCT TTGCGTCAGT CATTCCGCCG CATTAGGAGG 
AGTCAAGTCA ACAAGAGAAG ACCAGGGCCT GATAGGGTGC AAGAGGCCAA TGCTCGATTT 
GAGCAGGAAG CTCTGCAAGA GATGGAACTG GCCCCCGTGC AGAGGCGGAT AGAGGCCCGC 
TCGGCTGAGG ATTCCTTCAC CGGATTCGTG CGCACATTGT ACTTTGCCGA CACCTACCTG 
AGGGACAGCT CTAGGCACAC GCCTTCCCTC TGGGCGGGGA CAAATGGCGG CACAGTGTAT 
GCGTTCTCTT TGCGGGTGCC ACCCACAGAG CGCCGAATGG AAGAGGCAGT GGCGGCAACG 
CAGGCTAAGG AAATCCAACT CATGCACCGG GCTCCGGTTG TGGGGATCCT GGTTCTGGAC 
GGTCACAGCG TTCCTCTCCC AGAACCCTTA GAGGCAGCTC ACGATCTCTC CAAGAGCCCC 
GACATGCAAG GCAGCCACCA GCTATTGGTC ACTTCCGAGG AGCAGTTTAA GGTCTTTACT 
CTGCCCAAAG TGAGCAGCAA ACTGAAGCTA AAACTGACGG CCACGGACGG CTGCCGCGTG 
CGCAAGATGA CGGTGTGCAC CTACGGTAGC TGCAAGTCGG AGGAGCACAG CGAGAACCAC 
CTGACCATTC TTACCAACCT GGGGGATATT CAGGTGGTGT CACTGCCCCA TCTGAAGCCA 
CAAGTCCGGT ACGACTGCAT CCGCAAGGAA GATGTGAGCG GCATCGCCTC CTGTGTCTTC 
ACCAAACACG GGCAGGGGTT TTATCTAATC TCCCCCTCTG AGTTGGAACG GTTTTCGCTA 
TCCACATGCT GGTTGGTGGC TCCCCGTGCC AGAGTGGATC CTCCGGAGCC CCCTCGTAAC 
TCGCGGCTAC GGCGGGGGGG CAGCACTGAC CAACCAACTG ACAGGTCCAG GTCTTCTGGG 
ACGGACCACA CAGGAGGCCA GCGGGCCCAG GGGACACTGA TGGAACATGC GCTGCTAAAT 
GACGAGAAGA TGCTGAAGGA AATCCAGAGC ACGCTGGAAG GAACCCGAAT AAGCTACAGC 
GAAAGGAGTT CCCGGCAGAT GAGCGTTGGA CACAGACTGA TCAATGGGGC AGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

llgl2 ( NM_001015691.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001015691.1 Xenopus tropicalis LLGL2, scribble cell polarity complex component (llgl2), mRNA.

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CloneID	OXa22108
Clone ID Related Accession (Same CDS sequence)	NM_001015691.1
Accession Version	NM_001015691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LLGL scribble cell polarity complex component 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY884236.1. ##Evidence-Data-START## Transcript exon combination :: AY884236.1, BC135909.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCGCT TCCTTCGCTC CGGCCATGAT TCTGTGAGGG AGCGGCTGAA ACGGGACCTT 
TTCCAGTTTA ACAAGACAGT GGAACATGGA TTCCCCCACC AGCCCAGCGC TTTGGGGTAC 
AGTGACTGCC TTAGCCTCAT GGCGATTGGA ACTCGTTCCG GAGCCCTCAA AGTATATGGC 
TCCCCAGGGG TGGAATTTAT GGGTCTCCAT GATGAGAATA ACTCTGTGAC AAAGATCAGC 
TTCATCCCAG ACCAGTGCCA ACTCGTCACC GTCCTCGATG ACAACAGCCT CCACCTGTGG 
TCATTTCCGC ATCACGACGG GGTCTCAGAA CTGCAGGAAG AGAAGAAATT CACCCTACGT 
GGCCCCCCTG GGTCTCCTCC GAGCGTGACG CAGATCAAGG TGCTGTTGCC GCACTCCTCG 
GGGGAGAAGC TGTACGTGGG CACCGAGGGG GGCAGTGTGT TCATTCTCGA GCTGCCATCT 
CTGCGGGTGC TAGAAGAGAA GACAGTGGGG CATGAAGCGG CTCTTCAAGG TCTGCCTGAC 
GAGTACAGCA GCAGGAGAGC GTTTGAGTCT GTGGAGGCCT TGCAGGAGAA TCCCCGCAAT 
CCAGACCAGC TTTTGATTGG TTACAGCCGC GGAATGATGG TGACGTGGGA CTTGGTAAAG 
GGACGCAGCG TGCAATATTT TCTGGGCAAA CAGCAACTAG AGAACGTTTT CTGGTGCCGC 
GATGGGAAGC AGATAGTCAG CTGTCACAGC GATGGGGGCT ACAACCAATG GGAGGTCTCA 
AATGAGCTAG TACAGGTTTC TCCCAAGGAG AGCAAAGTGC CATACGGTCC CTTCCGCTGC 
AAGGCAATCA GTAAAATAAC CTGGCACACC ACGCGCAGAA GCCGGTCTCC GTACGTGGTG 
TTCCAAGGAG GGATGCCCAT GGCCAGCTAT GGGGACAGGC ACATCATATC CGTGGTACAT 
GGGAACCAGC AGACTGCTTT TGACTTTACC TCTCGGATCA TTGACCACGT AGTTATTGCC 
AACCCGGATC CCTCAGCAGA GTACGATGAC CCGCGGGCCC TGCTGGTGCT GGTAGAAGAG 
GAGTTGGTGA TGATCGACCT GCAGAGCCCC GGCTGGCCAG TGATCCAGTG CCCCTACCTC 
ATCTCCCTCC ATTGTTCAGC CATTACCTGC TCCCATCACA TCTCCTCCAT CCCCTTGAAG 
CTATGGGAGC GGATAATCAG CGCGGGGAGG AAGCAGAACA CTCAGTACTC TAACATGCCG 
TGGCCCATTG ATGGTGGCGT CAATTTGGCT CCTGCGCCCT TTCAGAGGGA CCTGCTTCTT 
ACTGGACATG AGGATGGCAC TGTGCGCTTC TGGGATGCCT CCGGCATTTG CCTTCAGCTA 
ATGTACAAGA TGAGCACAGT GAGTGTCTTC CAGACCGAGT CGGACCACAA CGACAATGTC 
AACTCCAATG AGGAGGAAGA GTGGCCTCCA CTGAGGAAAG TTGGCTCCTT TGACCCTTAC 
AGTGATGACC CCAGGCTGGG CATACAGAAG ATTTACATGT GCAAATACAG TGGTTACCTC 
TCTGTGGCTG GCACGGCTGG ACAGGTTCTG GTTATGGAGC TGAATGATGA AGCTGCAGAG 
CATGTGGTGG ACGAGACCTT AGTAGACCTC CTTCAGGAAC AAGAAGGCTA CCGCTGGAAG 
GGCCACAAAC GGCTCCATGC TCGGGAGGAC CCTGTCCGCT TTCCACCGGG GTTCCAGCCA 
TTTGCCCTGG TGCAGTGCCA GCCCCCTGCT GCTGTTACTT CCCTGGCCCT GCACTCTGAG 
TGGCGGCTGT TGGCCTTTGG TACCAGCCAT GGCTTCGGCT TGTACGACTT TCAGCAGAAG 
CGCCAAGTAA TGGCCAAGTG CACGTTACAT GCCAGTGACC AAATGGCGTT GGAGGGTCCC 
CTGTCACGGG TTAAATCTCT GAAGAAATCT TTGCGTCAGT CATTCCGCCG CATTAGGAGG 
AGTCAAGTCA ACAAGAGAAG ACCAGGGCCT GATAGGGTGC AAGAGGCCAA TGCTCGATTT 
GAGCAGGAAG CTCTGCAAGA GATGGAACTG GCCCCCGTGC AGAGGCGGAT AGAGGCCCGC 
TCGGCTGAGG ATTCCTTCAC CGGATTCGTG CGCACATTGT ACTTTGCCGA CACCTACCTG 
AGGGACAGCT CTAGGCACAC GCCTTCCCTC TGGGCGGGGA CAAATGGCGG CACAGTGTAT 
GCGTTCTCTT TGCGGGTGCC ACCCACAGAG CGCCGAATGG AAGAGGCAGT GGCGGCAACG 
CAGGCTAAGG AAATCCAACT CATGCACCGG GCTCCGGTTG TGGGGATCCT GGTTCTGGAC 
GGTCACAGCG TTCCTCTCCC AGAACCCTTA GAGGCAGCTC ACGATCTCTC CAAGAGCCCC 
GACATGCAAG GCAGCCACCA GCTATTGGTC ACTTCCGAGG AGCAGTTTAA GGTCTTTACT 
CTGCCCAAAG TGAGCAGCAA ACTGAAGCTA AAACTGACGG CCACGGACGG CTGCCGCGTG 
CGCAAGATGA CGGTGTGCAC CTACGGTAGC TGCAAGTCGG AGGAGCACAG CGAGAACCAC 
CTGACCATTC TTACCAACCT GGGGGATATT CAGGTGGTGT CACTGCCCCA TCTGAAGCCA 
CAAGTCCGGT ACGACTGCAT CCGCAAGGAA GATGTGAGCG GCATCGCCTC CTGTGTCTTC 
ACCAAACACG GGGCAGGGGT TTTATCTAAT CTCCCCTCTG AGTTGGAACG GTTTTCGCTA 
TCCACATGCT GGTTGGTGGC TCCCCGTGCC AGAGTGGATC CTCCGGAGCC CCCTCGTAAC 
TCGCGGCTAC GGCGGGGGGG CAGCACTGAC CAACCAACTG ACAGGTCCAG GTCTTCTGGG 
ACGGACCACA CAGGAGGCCA GCGGGCCCAG GGGACACTGA TGGAACATGC GCTGCTAAAT 
GACGAGAAGA TGCTGAAGGA AATCCAGAGC ACGCTGGAAG GAACCCGAAT AAGCTACAGC 
GAAAGGAGTT CCCGGCAGAT GAGCGTTGGA CACAGACTGA TCAATGGGGC AGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001015691.1
CDS	80..3139
Translation	MKRFLRSGHDSVRERLKRDLFQFNKTVEHGFPHQPSALGYSDCLSLMAIGTRSGALKVYGSPGVEFMGLHDENNSVTKISFIPDQCQLVTVLDDNSLHLWSFPHHDGVSELQEEKKFTLRGPPGSPPSVTQIKVLLPHSSGEKLYVGTEGGSVFILELPSLRVLEEKTVGHEAALQGLPDEYSSRRAFESVEALQENPRNPDQLLIGYSRGMMVTWDLVKGRSVQYFLGKQQLENVFWCRDGKQIVSCHSDGGYNQWEVSNELVQVSPKESKVPYGPFRCKAISKITWHTTRRSRSPYVVFQGGMPMASYGDRHIISVVHGNQQTAFDFTSRIIDHVVIANPDPSAEYDDPRALLVLVEEELVMIDLQSPGWPVIQCPYLISLHCSAITCSHHISSIPLKLWERIISAGRKQNTQYSNMPWPIDGGVNLAPAPFQRDLLLTGHEDGTVRFWDASGICLQLMYKMSTVSVFQTESDHNDNVNSNEEEEWPPLRKVGSFDPYSDDPRLGIQKIYMCKYSGYLSVAGTAGQVLVMELNDEAAEHVVDETLVDLLQEQEGYRWKGHKRLHAREDPVRFPPGFQPFALVQCQPPAAVTSLALHSEWRLLAFGTSHGFGLYDFQQKRQVMAKCTLHASDQMALEGPLSRVKSLKKSLRQSFRRIRRSQVNKRRPGPDRVQEANARFEQEALQEMELAPVQRRIEARSAEDSFTGFVRTLYFADTYLRDSSRHTPSLWAGTNGGTVYAFSLRVPPTERRMEEAVAATQAKEIQLMHRAPVVGILVLDGHSVPLPEPLEAAHDLSKSPDMQGSHQLLVTSEEQFKVFTLPKVSSKLKLKLTATDGCRVRKMTVCTYGSCKSEEHSENHLTILTNLGDIQVVSLPHLKPQVRYDCIRKEDVSGIASCVFTKHGAGVLSNLPSELERFSLSTCWLVAPRARVDPPEPPRNSRLRRGGSTDQPTDRSRSSGTDHTGGQRAQGTLMEHALLNDEKMLKEIQSTLEGTRISYSERSSRQMSVGHRLINGAAE

Target ORF information:

RefSeq Version	NM_001015691.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis LLGL2, scribble cell polarity complex component (llgl2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001015691.1

ORF Insert Sequence:

ATGAAGCGCT TCCTTCGCTC CGGCCATGAT TCTGTGAGGG AGCGGCTGAA ACGGGACCTT 
TTCCAGTTTA ACAAGACAGT GGAACATGGA TTCCCCCACC AGCCCAGCGC TTTGGGGTAC 
AGTGACTGCC TTAGCCTCAT GGCGATTGGA ACTCGTTCCG GAGCCCTCAA AGTATATGGC 
TCCCCAGGGG TGGAATTTAT GGGTCTCCAT GATGAGAATA ACTCTGTGAC AAAGATCAGC 
TTCATCCCAG ACCAGTGCCA ACTCGTCACC GTCCTCGATG ACAACAGCCT CCACCTGTGG 
TCATTTCCGC ATCACGACGG GGTCTCAGAA CTGCAGGAAG AGAAGAAATT CACCCTACGT 
GGCCCCCCTG GGTCTCCTCC GAGCGTGACG CAGATCAAGG TGCTGTTGCC GCACTCCTCG 
GGGGAGAAGC TGTACGTGGG CACCGAGGGG GGCAGTGTGT TCATTCTCGA GCTGCCATCT 
CTGCGGGTGC TAGAAGAGAA GACAGTGGGG CATGAAGCGG CTCTTCAAGG TCTGCCTGAC 
GAGTACAGCA GCAGGAGAGC GTTTGAGTCT GTGGAGGCCT TGCAGGAGAA TCCCCGCAAT 
CCAGACCAGC TTTTGATTGG TTACAGCCGC GGAATGATGG TGACGTGGGA CTTGGTAAAG 
GGACGCAGCG TGCAATATTT TCTGGGCAAA CAGCAACTAG AGAACGTTTT CTGGTGCCGC 
GATGGGAAGC AGATAGTCAG CTGTCACAGC GATGGGGGCT ACAACCAATG GGAGGTCTCA 
AATGAGCTAG TACAGGTTTC TCCCAAGGAG AGCAAAGTGC CATACGGTCC CTTCCGCTGC 
AAGGCAATCA GTAAAATAAC CTGGCACACC ACGCGCAGAA GCCGGTCTCC GTACGTGGTG 
TTCCAAGGAG GGATGCCCAT GGCCAGCTAT GGGGACAGGC ACATCATATC CGTGGTACAT 
GGGAACCAGC AGACTGCTTT TGACTTTACC TCTCGGATCA TTGACCACGT AGTTATTGCC 
AACCCGGATC CCTCAGCAGA GTACGATGAC CCGCGGGCCC TGCTGGTGCT GGTAGAAGAG 
GAGTTGGTGA TGATCGACCT GCAGAGCCCC GGCTGGCCAG TGATCCAGTG CCCCTACCTC 
ATCTCCCTCC ATTGTTCAGC CATTACCTGC TCCCATCACA TCTCCTCCAT CCCCTTGAAG 
CTATGGGAGC GGATAATCAG CGCGGGGAGG AAGCAGAACA CTCAGTACTC TAACATGCCG 
TGGCCCATTG ATGGTGGCGT CAATTTGGCT CCTGCGCCCT TTCAGAGGGA CCTGCTTCTT 
ACTGGACATG AGGATGGCAC TGTGCGCTTC TGGGATGCCT CCGGCATTTG CCTTCAGCTA 
ATGTACAAGA TGAGCACAGT GAGTGTCTTC CAGACCGAGT CGGACCACAA CGACAATGTC 
AACTCCAATG AGGAGGAAGA GTGGCCTCCA CTGAGGAAAG TTGGCTCCTT TGACCCTTAC 
AGTGATGACC CCAGGCTGGG CATACAGAAG ATTTACATGT GCAAATACAG TGGTTACCTC 
TCTGTGGCTG GCACGGCTGG ACAGGTTCTG GTTATGGAGC TGAATGATGA AGCTGCAGAG 
CATGTGGTGG ACGAGACCTT AGTAGACCTC CTTCAGGAAC AAGAAGGCTA CCGCTGGAAG 
GGCCACAAAC GGCTCCATGC TCGGGAGGAC CCTGTCCGCT TTCCACCGGG GTTCCAGCCA 
TTTGCCCTGG TGCAGTGCCA GCCCCCTGCT GCTGTTACTT CCCTGGCCCT GCACTCTGAG 
TGGCGGCTGT TGGCCTTTGG TACCAGCCAT GGCTTCGGCT TGTACGACTT TCAGCAGAAG 
CGCCAAGTAA TGGCCAAGTG CACGTTACAT GCCAGTGACC AAATGGCGTT GGAGGGTCCC 
CTGTCACGGG TTAAATCTCT GAAGAAATCT TTGCGTCAGT CATTCCGCCG CATTAGGAGG 
AGTCAAGTCA ACAAGAGAAG ACCAGGGCCT GATAGGGTGC AAGAGGCCAA TGCTCGATTT 
GAGCAGGAAG CTCTGCAAGA GATGGAACTG GCCCCCGTGC AGAGGCGGAT AGAGGCCCGC 
TCGGCTGAGG ATTCCTTCAC CGGATTCGTG CGCACATTGT ACTTTGCCGA CACCTACCTG 
AGGGACAGCT CTAGGCACAC GCCTTCCCTC TGGGCGGGGA CAAATGGCGG CACAGTGTAT 
GCGTTCTCTT TGCGGGTGCC ACCCACAGAG CGCCGAATGG AAGAGGCAGT GGCGGCAACG 
CAGGCTAAGG AAATCCAACT CATGCACCGG GCTCCGGTTG TGGGGATCCT GGTTCTGGAC 
GGTCACAGCG TTCCTCTCCC AGAACCCTTA GAGGCAGCTC ACGATCTCTC CAAGAGCCCC 
GACATGCAAG GCAGCCACCA GCTATTGGTC ACTTCCGAGG AGCAGTTTAA GGTCTTTACT 
CTGCCCAAAG TGAGCAGCAA ACTGAAGCTA AAACTGACGG CCACGGACGG CTGCCGCGTG 
CGCAAGATGA CGGTGTGCAC CTACGGTAGC TGCAAGTCGG AGGAGCACAG CGAGAACCAC 
CTGACCATTC TTACCAACCT GGGGGATATT CAGGTGGTGT CACTGCCCCA TCTGAAGCCA 
CAAGTCCGGT ACGACTGCAT CCGCAAGGAA GATGTGAGCG GCATCGCCTC CTGTGTCTTC 
ACCAAACACG GGGCAGGGGT TTTATCTAAT CTCCCCTCTG AGTTGGAACG GTTTTCGCTA 
TCCACATGCT GGTTGGTGGC TCCCCGTGCC AGAGTGGATC CTCCGGAGCC CCCTCGTAAC 
TCGCGGCTAC GGCGGGGGGG CAGCACTGAC CAACCAACTG ACAGGTCCAG GTCTTCTGGG 
ACGGACCACA CAGGAGGCCA GCGGGCCCAG GGGACACTGA TGGAACATGC GCTGCTAAAT 
GACGAGAAGA TGCTGAAGGA AATCCAGAGC ACGCTGGAAG GAACCCGAAT AAGCTACAGC 
GAAAGGAGTT CCCGGCAGAT GAGCGTTGGA CACAGACTGA TCAATGGGGC AGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

llgl2 ( XM_012971730.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827184.1 Xenopus tropicalis lethal giant larvae homolog 2 (llgl2), transcript variant X2, mRNA.

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CloneID	OXa27348
Clone ID Related Accession (Same CDS sequence)	XM_012971730.2 , XM_012971729.3 , XM_031894071.1 , XM_012971729.2
Accession Version	XM_012971730.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lethal(2) giant larvae protein homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971730.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGCT TCCTTCGCTC CGGCCATGAT TCTGTGAGGG AGCGGCTGAA ACGGGACCTT 
TTCCAGTTTA ACAAGACAGT GGAACATGGA TTCCCCCACC AGCCCAGCGC TTTGGGGTAC 
AGTGACTGCC TTAGCCTCAT GGCGATTGGA ACTCGTTCCG GAGCCCTCAA AGTATATGGC 
TCCCCAGGGG TGGAATTTAT GGGTCTCCAT GATGAGAATA ACTCTGTGAC AAAGATCAGC 
TTCATCCCAG ACCAGTGCCA ACTCGTCACC GTCCTCGATG ACAACAGCCT CCACCTGTGG 
TCATTTCCGC ATCACGACGG GGTCTCAGAA CTGCAGGAAG AGAAGAAATT CACCCTACGT 
GGCCCCCCTG GGTCTCCTCC GAGCGTGACG CAGATCAAGG TGCTGTTGCC GCACTCCTCG 
GGGGAGAAGC TGTACGTGGG CACCGAGGGG GGCAGTGTGT TCATTCTCGA GCTGCCATCT 
CTGCGGGTGC TAGAAGAGAA GACAGTGGGG CATGAAGCGG CTCTTCAAGG TCTGCCTGAC 
GAGTACAGCA GCAGGAGAGC GTTTGAGTCT GTGGAGGCCT TGCAGGAGAA TCCCCGCAAT 
CCAGACCAGC TTTTGATTGG TTACAGCCGC GGAATGATGG TGACGTGGGA CTTGGTAAAG 
GGACGCAGCG TGCAATATTT TCTGGGCAAA CAGCAACTAG AGAACGTTTT CTGGTGCCGC 
GATGGGAAGC AGATAGTCAG CTGTCACAGC GATGGGGGCT ACAACCAATG GGAGGTCTCA 
AATGAGCTAG TACAGGTTTC TCCCAAGGAG AGCAAAGTGC CATACGGTCC CTTCCGCTGC 
AAGGCAATCA GTAAAATAAC CTGGCACACC ACGCGCAGAA GCCGGTCTCC GTACGTGGTG 
TTCCAAGGAG GGATGCCCAT GGCCAGCTAT GGGGACAGGC ACATCATATC CGTGGTACAT 
GGGAACCAGC AGACTGCTTT TGACTTTACC TCTCGGATCA TTGACCACGT AGTTATTGCC 
AACCCGGATC CCTCAGCAGA GTACGATGAC CCGCGGGCCC TGCTGGTGCT GGTAGAAGAG 
GAGTTGGTGA TGATCGACCT GCAGAGCCCC GGCTGGCCAG TGATCCAGTG CCCCTACCTC 
ATCTCCCTCC ATTGTTCAGC CATTACCTGC TCCCATCACA TCTCCTCCAT CCCCTTGAAG 
CTATGGGAGC GGATAATCAG CGCGGGGAGG AAGCAGAACA CTCAGTACTC TAACATGCCG 
TGGCCCATTG ATGGTGGCGT CAATTTGGCT CCTGCGCCCC CTCAGAGGGA CCTGCTTCTT 
ACTGGACATG AGGATGGCAC TGTGCGCTTC TGGGATGCCT CCGGCATTTG CCTTCAGCTA 
ATGTACAAGA TGAGCACAGT GAGTGTCTTC CAGACCGAGT CGGACCACAA CGACAATGTC 
AACTCCAATG AGGAGGAAGA GTGGCCTCCA CTGAGGAAAG TTGGCTCCTT TGACCCTTAC 
AGTGATGACC CCAGGCTGGG CATACAGAAG ATTTACATGT GCAAATACAG TGGTTACCTC 
TCTGTGGCTG GCACGGCTGG ACAGGTTCTG GTTATGGAGC TGAATGATGA AGCTGCAGAG 
CATGTGGTGG ACGAGACCTT AGTAGACCTC CTTCAGGAAC AAGAAGGCTA CCGCTGGAAG 
GGCCACGAAC GGCTCCATGC TCGGGAGGAC CCTGTCCGCT TTCCACCGGG GTTCCAGCCA 
TTTGCCCTGG TGCAGTGCCA GCCCCCTGCT GCTGTTACTT CCCTGGCCCT GCACTCTGAG 
TGGCGGCTGT TGGCCTTTGG TACCAGCCAT GGCTTCGGCT TGTACGACTT TCAGCAGAAG 
CGCCAAGTAA TGGCCAAGTG CACGTTACAT GCCAGTGACC AAATGGCGTT GGAGGGTCCC 
CTGTCACGGG TTAAATCTCT GAAGAAATCT TTGCGTCAGT CATTCCGCCG CATTAGGAGG 
AGTCAAGTCA ACAAGAGAAG ACCAGGGCCT GATAGGGTGC AAGAGGCCAA TGCTCGATTT 
GAGCAGGAAG CTCTGCAAGA GATGGAACTG GCCCCCGTGC AGAGGCGGAT AGAGGCCCGC 
TCGGCTGAGG ATTCCTTCAC CGGATTCGTG CGCACATTGT ACTTTGCCGA CACCTACCTG 
AGGGACAGCT CTAGGCACAC GCCTTCCCTC TGGGCGGGGA CAAATGGCGG CACAGTGTAT 
GCGTTCTCTT TGCGGGTGCC ACCCACAGAG CGCCGAATGG AAGAGGCAGT GGCGGCAACG 
CAGGCTAAGG AAATCCAACT CATGCACCGG GCTCCGGTTG TGGGGATCCT GGTTCTGGAC 
GGTCACAGCG TTCCTCTCCC AGAACCCTTA GAGGCAGCTC ACGATCTCTC CAAGAGCCCC 
GACATGCAAG GCAGCCACCA GCTATTGGTC ACTTCCGAGG AGCAGTTTAA GGTCTTTACT 
CTGCCCAAAG TGAGCAGCAA ACTGAAGCTA AAACTGACGG CCACGGACGG CTGCCGCGTG 
CGCAAGATGA CGGTGTGCAC CTACGGTAGC TGCAAGTCGG AGGAGCACAG CGAGAACCAC 
CTGACCATTC TTACCAACCT GGGGGATATT CAGGTGGTGT CACTGCCCCA TCTGAAGCCA 
CAAGTCCGGT ACGACTGCAT CCGCAAGGAA GATGTGAGCG GCATCGCCTC CTGTGTCTTC 
ACCAAACACG GGCAGGGGTT TTATCTAATC TCCCCCTCTG AGTTGGAACG GTTTTCGCTA 
TCCACATGCT GGTTGGTGGC TCCCCGTGCC AGAGTGGATC CTCCGGAGCC CCCTCGTAAC 
TCGCGGCTAC GGCGGGGGGG CAGCACTGAC CAACCAACTG ACAGGTCCAG GTCTTCTGGG 
ACGGACCACA CAGGAGGCCA GCGGGCCCAG GGGACACTGA TGGAACATGC GCTGCTAAAT 
GACGAGAAGA TGCTGAAGGA AATCCAGAGC ACGCTGGAAG GAACCCGAAT AAGCTACAGC 
GAAAGGAGTT CCCGGCAGAT GAGCGTTGGA CACAGACTGA TCAATGGGGC AGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827184.1
CDS	44..3103
Translation	MKRFLRSGHDSVRERLKRDLFQFNKTVEHGFPHQPSALGYSDCLSLMAIGTRSGALKVYGSPGVEFMGLHDENNSVTKISFIPDQCQLVTVLDDNSLHLWSFPHHDGVSELQEEKKFTLRGPPGSPPSVTQIKVLLPHSSGEKLYVGTEGGSVFILELPSLRVLEEKTVGHEAALQGLPDEYSSRRAFESVEALQENPRNPDQLLIGYSRGMMVTWDLVKGRSVQYFLGKQQLENVFWCRDGKQIVSCHSDGGYNQWEVSNELVQVSPKESKVPYGPFRCKAISKITWHTTRRSRSPYVVFQGGMPMASYGDRHIISVVHGNQQTAFDFTSRIIDHVVIANPDPSAEYDDPRALLVLVEEELVMIDLQSPGWPVIQCPYLISLHCSAITCSHHISSIPLKLWERIISAGRKQNTQYSNMPWPIDGGVNLAPAPPQRDLLLTGHEDGTVRFWDASGICLQLMYKMSTVSVFQTESDHNDNVNSNEEEEWPPLRKVGSFDPYSDDPRLGIQKIYMCKYSGYLSVAGTAGQVLVMELNDEAAEHVVDETLVDLLQEQEGYRWKGHERLHAREDPVRFPPGFQPFALVQCQPPAAVTSLALHSEWRLLAFGTSHGFGLYDFQQKRQVMAKCTLHASDQMALEGPLSRVKSLKKSLRQSFRRIRRSQVNKRRPGPDRVQEANARFEQEALQEMELAPVQRRIEARSAEDSFTGFVRTLYFADTYLRDSSRHTPSLWAGTNGGTVYAFSLRVPPTERRMEEAVAATQAKEIQLMHRAPVVGILVLDGHSVPLPEPLEAAHDLSKSPDMQGSHQLLVTSEEQFKVFTLPKVSSKLKLKLTATDGCRVRKMTVCTYGSCKSEEHSENHLTILTNLGDIQVVSLPHLKPQVRYDCIRKEDVSGIASCVFTKHGQGFYLISPSELERFSLSTCWLVAPRARVDPPEPPRNSRLRRGGSTDQPTDRSRSSGTDHTGGQRAQGTLMEHALLNDEKMLKEIQSTLEGTRISYSERSSRQMSVGHRLINGAAE

Target ORF information:

RefSeq Version	XM_012971730.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lethal giant larvae homolog 2 (llgl2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971730.2

ORF Insert Sequence:

ATGAAGCGCT TCCTTCGCTC CGGCCATGAT TCTGTGAGGG AGCGGCTGAA ACGGGACCTT 
TTCCAGTTTA ACAAGACAGT GGAACATGGA TTCCCCCACC AGCCCAGCGC TTTGGGGTAC 
AGTGACTGCC TTAGCCTCAT GGCGATTGGA ACTCGTTCCG GAGCCCTCAA AGTATATGGC 
TCCCCAGGGG TGGAATTTAT GGGTCTCCAT GATGAGAATA ACTCTGTGAC AAAGATCAGC 
TTCATCCCAG ACCAGTGCCA ACTCGTCACC GTCCTCGATG ACAACAGCCT CCACCTGTGG 
TCATTTCCGC ATCACGACGG GGTCTCAGAA CTGCAGGAAG AGAAGAAATT CACCCTACGT 
GGCCCCCCTG GGTCTCCTCC GAGCGTGACG CAGATCAAGG TGCTGTTGCC GCACTCCTCG 
GGGGAGAAGC TGTACGTGGG CACCGAGGGG GGCAGTGTGT TCATTCTCGA GCTGCCATCT 
CTGCGGGTGC TAGAAGAGAA GACAGTGGGG CATGAAGCGG CTCTTCAAGG TCTGCCTGAC 
GAGTACAGCA GCAGGAGAGC GTTTGAGTCT GTGGAGGCCT TGCAGGAGAA TCCCCGCAAT 
CCAGACCAGC TTTTGATTGG TTACAGCCGC GGAATGATGG TGACGTGGGA CTTGGTAAAG 
GGACGCAGCG TGCAATATTT TCTGGGCAAA CAGCAACTAG AGAACGTTTT CTGGTGCCGC 
GATGGGAAGC AGATAGTCAG CTGTCACAGC GATGGGGGCT ACAACCAATG GGAGGTCTCA 
AATGAGCTAG TACAGGTTTC TCCCAAGGAG AGCAAAGTGC CATACGGTCC CTTCCGCTGC 
AAGGCAATCA GTAAAATAAC CTGGCACACC ACGCGCAGAA GCCGGTCTCC GTACGTGGTG 
TTCCAAGGAG GGATGCCCAT GGCCAGCTAT GGGGACAGGC ACATCATATC CGTGGTACAT 
GGGAACCAGC AGACTGCTTT TGACTTTACC TCTCGGATCA TTGACCACGT AGTTATTGCC 
AACCCGGATC CCTCAGCAGA GTACGATGAC CCGCGGGCCC TGCTGGTGCT GGTAGAAGAG 
GAGTTGGTGA TGATCGACCT GCAGAGCCCC GGCTGGCCAG TGATCCAGTG CCCCTACCTC 
ATCTCCCTCC ATTGTTCAGC CATTACCTGC TCCCATCACA TCTCCTCCAT CCCCTTGAAG 
CTATGGGAGC GGATAATCAG CGCGGGGAGG AAGCAGAACA CTCAGTACTC TAACATGCCG 
TGGCCCATTG ATGGTGGCGT CAATTTGGCT CCTGCGCCCC CTCAGAGGGA CCTGCTTCTT 
ACTGGACATG AGGATGGCAC TGTGCGCTTC TGGGATGCCT CCGGCATTTG CCTTCAGCTA 
ATGTACAAGA TGAGCACAGT GAGTGTCTTC CAGACCGAGT CGGACCACAA CGACAATGTC 
AACTCCAATG AGGAGGAAGA GTGGCCTCCA CTGAGGAAAG TTGGCTCCTT TGACCCTTAC 
AGTGATGACC CCAGGCTGGG CATACAGAAG ATTTACATGT GCAAATACAG TGGTTACCTC 
TCTGTGGCTG GCACGGCTGG ACAGGTTCTG GTTATGGAGC TGAATGATGA AGCTGCAGAG 
CATGTGGTGG ACGAGACCTT AGTAGACCTC CTTCAGGAAC AAGAAGGCTA CCGCTGGAAG 
GGCCACGAAC GGCTCCATGC TCGGGAGGAC CCTGTCCGCT TTCCACCGGG GTTCCAGCCA 
TTTGCCCTGG TGCAGTGCCA GCCCCCTGCT GCTGTTACTT CCCTGGCCCT GCACTCTGAG 
TGGCGGCTGT TGGCCTTTGG TACCAGCCAT GGCTTCGGCT TGTACGACTT TCAGCAGAAG 
CGCCAAGTAA TGGCCAAGTG CACGTTACAT GCCAGTGACC AAATGGCGTT GGAGGGTCCC 
CTGTCACGGG TTAAATCTCT GAAGAAATCT TTGCGTCAGT CATTCCGCCG CATTAGGAGG 
AGTCAAGTCA ACAAGAGAAG ACCAGGGCCT GATAGGGTGC AAGAGGCCAA TGCTCGATTT 
GAGCAGGAAG CTCTGCAAGA GATGGAACTG GCCCCCGTGC AGAGGCGGAT AGAGGCCCGC 
TCGGCTGAGG ATTCCTTCAC CGGATTCGTG CGCACATTGT ACTTTGCCGA CACCTACCTG 
AGGGACAGCT CTAGGCACAC GCCTTCCCTC TGGGCGGGGA CAAATGGCGG CACAGTGTAT 
GCGTTCTCTT TGCGGGTGCC ACCCACAGAG CGCCGAATGG AAGAGGCAGT GGCGGCAACG 
CAGGCTAAGG AAATCCAACT CATGCACCGG GCTCCGGTTG TGGGGATCCT GGTTCTGGAC 
GGTCACAGCG TTCCTCTCCC AGAACCCTTA GAGGCAGCTC ACGATCTCTC CAAGAGCCCC 
GACATGCAAG GCAGCCACCA GCTATTGGTC ACTTCCGAGG AGCAGTTTAA GGTCTTTACT 
CTGCCCAAAG TGAGCAGCAA ACTGAAGCTA AAACTGACGG CCACGGACGG CTGCCGCGTG 
CGCAAGATGA CGGTGTGCAC CTACGGTAGC TGCAAGTCGG AGGAGCACAG CGAGAACCAC 
CTGACCATTC TTACCAACCT GGGGGATATT CAGGTGGTGT CACTGCCCCA TCTGAAGCCA 
CAAGTCCGGT ACGACTGCAT CCGCAAGGAA GATGTGAGCG GCATCGCCTC CTGTGTCTTC 
ACCAAACACG GGCAGGGGTT TTATCTAATC TCCCCCTCTG AGTTGGAACG GTTTTCGCTA 
TCCACATGCT GGTTGGTGGC TCCCCGTGCC AGAGTGGATC CTCCGGAGCC CCCTCGTAAC 
TCGCGGCTAC GGCGGGGGGG CAGCACTGAC CAACCAACTG ACAGGTCCAG GTCTTCTGGG 
ACGGACCACA CAGGAGGCCA GCGGGCCCAG GGGACACTGA TGGAACATGC GCTGCTAAAT 
GACGAGAAGA TGCTGAAGGA AATCCAGAGC ACGCTGGAAG GAACCCGAAT AAGCTACAGC 
GAAAGGAGTT CCCGGCAGAT GAGCGTTGGA CACAGACTGA TCAATGGGGC AGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

llgl2 ( XM_012971729.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827183.1 Xenopus tropicalis lethal giant larvae homolog 2 (llgl2), transcript variant X1, mRNA.

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CloneID	OXa27348
Clone ID Related Accession (Same CDS sequence)	XM_012971730.2 , XM_012971729.3 , XM_031894071.1 , XM_012971729.2
Accession Version	XM_012971729.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lethal(2) giant larvae protein homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971729.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGCT TCCTTCGCTC CGGCCATGAT TCTGTGAGGG AGCGGCTGAA ACGGGACCTT 
TTCCAGTTTA ACAAGACAGT GGAACATGGA TTCCCCCACC AGCCCAGCGC TTTGGGGTAC 
AGTGACTGCC TTAGCCTCAT GGCGATTGGA ACTCGTTCCG GAGCCCTCAA AGTATATGGC 
TCCCCAGGGG TGGAATTTAT GGGTCTCCAT GATGAGAATA ACTCTGTGAC AAAGATCAGC 
TTCATCCCAG ACCAGTGCCA ACTCGTCACC GTCCTCGATG ACAACAGCCT CCACCTGTGG 
TCATTTCCGC ATCACGACGG GGTCTCAGAA CTGCAGGAAG AGAAGAAATT CACCCTACGT 
GGCCCCCCTG GGTCTCCTCC GAGCGTGACG CAGATCAAGG TGCTGTTGCC GCACTCCTCG 
GGGGAGAAGC TGTACGTGGG CACCGAGGGG GGCAGTGTGT TCATTCTCGA GCTGCCATCT 
CTGCGGGTGC TAGAAGAGAA GACAGTGGGG CATGAAGCGG CTCTTCAAGG TCTGCCTGAC 
GAGTACAGCA GCAGGAGAGC GTTTGAGTCT GTGGAGGCCT TGCAGGAGAA TCCCCGCAAT 
CCAGACCAGC TTTTGATTGG TTACAGCCGC GGAATGATGG TGACGTGGGA CTTGGTAAAG 
GGACGCAGCG TGCAATATTT TCTGGGCAAA CAGCAACTAG AGAACGTTTT CTGGTGCCGC 
GATGGGAAGC AGATAGTCAG CTGTCACAGC GATGGGGGCT ACAACCAATG GGAGGTCTCA 
AATGAGCTAG TACAGGTTTC TCCCAAGGAG AGCAAAGTGC CATACGGTCC CTTCCGCTGC 
AAGGCAATCA GTAAAATAAC CTGGCACACC ACGCGCAGAA GCCGGTCTCC GTACGTGGTG 
TTCCAAGGAG GGATGCCCAT GGCCAGCTAT GGGGACAGGC ACATCATATC CGTGGTACAT 
GGGAACCAGC AGACTGCTTT TGACTTTACC TCTCGGATCA TTGACCACGT AGTTATTGCC 
AACCCGGATC CCTCAGCAGA GTACGATGAC CCGCGGGCCC TGCTGGTGCT GGTAGAAGAG 
GAGTTGGTGA TGATCGACCT GCAGAGCCCC GGCTGGCCAG TGATCCAGTG CCCCTACCTC 
ATCTCCCTCC ATTGTTCAGC CATTACCTGC TCCCATCACA TCTCCTCCAT CCCCTTGAAG 
CTATGGGAGC GGATAATCAG CGCGGGGAGG AAGCAGAACA CTCAGTACTC TAACATGCCG 
TGGCCCATTG ATGGTGGCGT CAATTTGGCT CCTGCGCCCC CTCAGAGGGA CCTGCTTCTT 
ACTGGACATG AGGATGGCAC TGTGCGCTTC TGGGATGCCT CCGGCATTTG CCTTCAGCTA 
ATGTACAAGA TGAGCACAGT GAGTGTCTTC CAGACCGAGT CGGACCACAA CGACAATGTC 
AACTCCAATG AGGAGGAAGA GTGGCCTCCA CTGAGGAAAG TTGGCTCCTT TGACCCTTAC 
AGTGATGACC CCAGGCTGGG CATACAGAAG ATTTACATGT GCAAATACAG TGGTTACCTC 
TCTGTGGCTG GCACGGCTGG ACAGGTTCTG GTTATGGAGC TGAATGATGA AGCTGCAGAG 
CATGTGGTGG ACGAGACCTT AGTAGACCTC CTTCAGGAAC AAGAAGGCTA CCGCTGGAAG 
GGCCACGAAC GGCTCCATGC TCGGGAGGAC CCTGTCCGCT TTCCACCGGG GTTCCAGCCA 
TTTGCCCTGG TGCAGTGCCA GCCCCCTGCT GCTGTTACTT CCCTGGCCCT GCACTCTGAG 
TGGCGGCTGT TGGCCTTTGG TACCAGCCAT GGCTTCGGCT TGTACGACTT TCAGCAGAAG 
CGCCAAGTAA TGGCCAAGTG CACGTTACAT GCCAGTGACC AAATGGCGTT GGAGGGTCCC 
CTGTCACGGG TTAAATCTCT GAAGAAATCT TTGCGTCAGT CATTCCGCCG CATTAGGAGG 
AGTCAAGTCA ACAAGAGAAG ACCAGGGCCT GATAGGGTGC AAGAGGCCAA TGCTCGATTT 
GAGCAGGAAG CTCTGCAAGA GATGGAACTG GCCCCCGTGC AGAGGCGGAT AGAGGCCCGC 
TCGGCTGAGG ATTCCTTCAC CGGATTCGTG CGCACATTGT ACTTTGCCGA CACCTACCTG 
AGGGACAGCT CTAGGCACAC GCCTTCCCTC TGGGCGGGGA CAAATGGCGG CACAGTGTAT 
GCGTTCTCTT TGCGGGTGCC ACCCACAGAG CGCCGAATGG AAGAGGCAGT GGCGGCAACG 
CAGGCTAAGG AAATCCAACT CATGCACCGG GCTCCGGTTG TGGGGATCCT GGTTCTGGAC 
GGTCACAGCG TTCCTCTCCC AGAACCCTTA GAGGCAGCTC ACGATCTCTC CAAGAGCCCC 
GACATGCAAG GCAGCCACCA GCTATTGGTC ACTTCCGAGG AGCAGTTTAA GGTCTTTACT 
CTGCCCAAAG TGAGCAGCAA ACTGAAGCTA AAACTGACGG CCACGGACGG CTGCCGCGTG 
CGCAAGATGA CGGTGTGCAC CTACGGTAGC TGCAAGTCGG AGGAGCACAG CGAGAACCAC 
CTGACCATTC TTACCAACCT GGGGGATATT CAGGTGGTGT CACTGCCCCA TCTGAAGCCA 
CAAGTCCGGT ACGACTGCAT CCGCAAGGAA GATGTGAGCG GCATCGCCTC CTGTGTCTTC 
ACCAAACACG GGCAGGGGTT TTATCTAATC TCCCCCTCTG AGTTGGAACG GTTTTCGCTA 
TCCACATGCT GGTTGGTGGC TCCCCGTGCC AGAGTGGATC CTCCGGAGCC CCCTCGTAAC 
TCGCGGCTAC GGCGGGGGGG CAGCACTGAC CAACCAACTG ACAGGTCCAG GTCTTCTGGG 
ACGGACCACA CAGGAGGCCA GCGGGCCCAG GGGACACTGA TGGAACATGC GCTGCTAAAT 
GACGAGAAGA TGCTGAAGGA AATCCAGAGC ACGCTGGAAG GAACCCGAAT AAGCTACAGC 
GAAAGGAGTT CCCGGCAGAT GAGCGTTGGA CACAGACTGA TCAATGGGGC AGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827183.1
CDS	102..3161
Translation	MKRFLRSGHDSVRERLKRDLFQFNKTVEHGFPHQPSALGYSDCLSLMAIGTRSGALKVYGSPGVEFMGLHDENNSVTKISFIPDQCQLVTVLDDNSLHLWSFPHHDGVSELQEEKKFTLRGPPGSPPSVTQIKVLLPHSSGEKLYVGTEGGSVFILELPSLRVLEEKTVGHEAALQGLPDEYSSRRAFESVEALQENPRNPDQLLIGYSRGMMVTWDLVKGRSVQYFLGKQQLENVFWCRDGKQIVSCHSDGGYNQWEVSNELVQVSPKESKVPYGPFRCKAISKITWHTTRRSRSPYVVFQGGMPMASYGDRHIISVVHGNQQTAFDFTSRIIDHVVIANPDPSAEYDDPRALLVLVEEELVMIDLQSPGWPVIQCPYLISLHCSAITCSHHISSIPLKLWERIISAGRKQNTQYSNMPWPIDGGVNLAPAPPQRDLLLTGHEDGTVRFWDASGICLQLMYKMSTVSVFQTESDHNDNVNSNEEEEWPPLRKVGSFDPYSDDPRLGIQKIYMCKYSGYLSVAGTAGQVLVMELNDEAAEHVVDETLVDLLQEQEGYRWKGHERLHAREDPVRFPPGFQPFALVQCQPPAAVTSLALHSEWRLLAFGTSHGFGLYDFQQKRQVMAKCTLHASDQMALEGPLSRVKSLKKSLRQSFRRIRRSQVNKRRPGPDRVQEANARFEQEALQEMELAPVQRRIEARSAEDSFTGFVRTLYFADTYLRDSSRHTPSLWAGTNGGTVYAFSLRVPPTERRMEEAVAATQAKEIQLMHRAPVVGILVLDGHSVPLPEPLEAAHDLSKSPDMQGSHQLLVTSEEQFKVFTLPKVSSKLKLKLTATDGCRVRKMTVCTYGSCKSEEHSENHLTILTNLGDIQVVSLPHLKPQVRYDCIRKEDVSGIASCVFTKHGQGFYLISPSELERFSLSTCWLVAPRARVDPPEPPRNSRLRRGGSTDQPTDRSRSSGTDHTGGQRAQGTLMEHALLNDEKMLKEIQSTLEGTRISYSERSSRQMSVGHRLINGAAE

Target ORF information:

RefSeq Version	XM_012971729.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lethal giant larvae homolog 2 (llgl2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971729.2

ORF Insert Sequence:

ATGAAGCGCT TCCTTCGCTC CGGCCATGAT TCTGTGAGGG AGCGGCTGAA ACGGGACCTT 
TTCCAGTTTA ACAAGACAGT GGAACATGGA TTCCCCCACC AGCCCAGCGC TTTGGGGTAC 
AGTGACTGCC TTAGCCTCAT GGCGATTGGA ACTCGTTCCG GAGCCCTCAA AGTATATGGC 
TCCCCAGGGG TGGAATTTAT GGGTCTCCAT GATGAGAATA ACTCTGTGAC AAAGATCAGC 
TTCATCCCAG ACCAGTGCCA ACTCGTCACC GTCCTCGATG ACAACAGCCT CCACCTGTGG 
TCATTTCCGC ATCACGACGG GGTCTCAGAA CTGCAGGAAG AGAAGAAATT CACCCTACGT 
GGCCCCCCTG GGTCTCCTCC GAGCGTGACG CAGATCAAGG TGCTGTTGCC GCACTCCTCG 
GGGGAGAAGC TGTACGTGGG CACCGAGGGG GGCAGTGTGT TCATTCTCGA GCTGCCATCT 
CTGCGGGTGC TAGAAGAGAA GACAGTGGGG CATGAAGCGG CTCTTCAAGG TCTGCCTGAC 
GAGTACAGCA GCAGGAGAGC GTTTGAGTCT GTGGAGGCCT TGCAGGAGAA TCCCCGCAAT 
CCAGACCAGC TTTTGATTGG TTACAGCCGC GGAATGATGG TGACGTGGGA CTTGGTAAAG 
GGACGCAGCG TGCAATATTT TCTGGGCAAA CAGCAACTAG AGAACGTTTT CTGGTGCCGC 
GATGGGAAGC AGATAGTCAG CTGTCACAGC GATGGGGGCT ACAACCAATG GGAGGTCTCA 
AATGAGCTAG TACAGGTTTC TCCCAAGGAG AGCAAAGTGC CATACGGTCC CTTCCGCTGC 
AAGGCAATCA GTAAAATAAC CTGGCACACC ACGCGCAGAA GCCGGTCTCC GTACGTGGTG 
TTCCAAGGAG GGATGCCCAT GGCCAGCTAT GGGGACAGGC ACATCATATC CGTGGTACAT 
GGGAACCAGC AGACTGCTTT TGACTTTACC TCTCGGATCA TTGACCACGT AGTTATTGCC 
AACCCGGATC CCTCAGCAGA GTACGATGAC CCGCGGGCCC TGCTGGTGCT GGTAGAAGAG 
GAGTTGGTGA TGATCGACCT GCAGAGCCCC GGCTGGCCAG TGATCCAGTG CCCCTACCTC 
ATCTCCCTCC ATTGTTCAGC CATTACCTGC TCCCATCACA TCTCCTCCAT CCCCTTGAAG 
CTATGGGAGC GGATAATCAG CGCGGGGAGG AAGCAGAACA CTCAGTACTC TAACATGCCG 
TGGCCCATTG ATGGTGGCGT CAATTTGGCT CCTGCGCCCC CTCAGAGGGA CCTGCTTCTT 
ACTGGACATG AGGATGGCAC TGTGCGCTTC TGGGATGCCT CCGGCATTTG CCTTCAGCTA 
ATGTACAAGA TGAGCACAGT GAGTGTCTTC CAGACCGAGT CGGACCACAA CGACAATGTC 
AACTCCAATG AGGAGGAAGA GTGGCCTCCA CTGAGGAAAG TTGGCTCCTT TGACCCTTAC 
AGTGATGACC CCAGGCTGGG CATACAGAAG ATTTACATGT GCAAATACAG TGGTTACCTC 
TCTGTGGCTG GCACGGCTGG ACAGGTTCTG GTTATGGAGC TGAATGATGA AGCTGCAGAG 
CATGTGGTGG ACGAGACCTT AGTAGACCTC CTTCAGGAAC AAGAAGGCTA CCGCTGGAAG 
GGCCACGAAC GGCTCCATGC TCGGGAGGAC CCTGTCCGCT TTCCACCGGG GTTCCAGCCA 
TTTGCCCTGG TGCAGTGCCA GCCCCCTGCT GCTGTTACTT CCCTGGCCCT GCACTCTGAG 
TGGCGGCTGT TGGCCTTTGG TACCAGCCAT GGCTTCGGCT TGTACGACTT TCAGCAGAAG 
CGCCAAGTAA TGGCCAAGTG CACGTTACAT GCCAGTGACC AAATGGCGTT GGAGGGTCCC 
CTGTCACGGG TTAAATCTCT GAAGAAATCT TTGCGTCAGT CATTCCGCCG CATTAGGAGG 
AGTCAAGTCA ACAAGAGAAG ACCAGGGCCT GATAGGGTGC AAGAGGCCAA TGCTCGATTT 
GAGCAGGAAG CTCTGCAAGA GATGGAACTG GCCCCCGTGC AGAGGCGGAT AGAGGCCCGC 
TCGGCTGAGG ATTCCTTCAC CGGATTCGTG CGCACATTGT ACTTTGCCGA CACCTACCTG 
AGGGACAGCT CTAGGCACAC GCCTTCCCTC TGGGCGGGGA CAAATGGCGG CACAGTGTAT 
GCGTTCTCTT TGCGGGTGCC ACCCACAGAG CGCCGAATGG AAGAGGCAGT GGCGGCAACG 
CAGGCTAAGG AAATCCAACT CATGCACCGG GCTCCGGTTG TGGGGATCCT GGTTCTGGAC 
GGTCACAGCG TTCCTCTCCC AGAACCCTTA GAGGCAGCTC ACGATCTCTC CAAGAGCCCC 
GACATGCAAG GCAGCCACCA GCTATTGGTC ACTTCCGAGG AGCAGTTTAA GGTCTTTACT 
CTGCCCAAAG TGAGCAGCAA ACTGAAGCTA AAACTGACGG CCACGGACGG CTGCCGCGTG 
CGCAAGATGA CGGTGTGCAC CTACGGTAGC TGCAAGTCGG AGGAGCACAG CGAGAACCAC 
CTGACCATTC TTACCAACCT GGGGGATATT CAGGTGGTGT CACTGCCCCA TCTGAAGCCA 
CAAGTCCGGT ACGACTGCAT CCGCAAGGAA GATGTGAGCG GCATCGCCTC CTGTGTCTTC 
ACCAAACACG GGCAGGGGTT TTATCTAATC TCCCCCTCTG AGTTGGAACG GTTTTCGCTA 
TCCACATGCT GGTTGGTGGC TCCCCGTGCC AGAGTGGATC CTCCGGAGCC CCCTCGTAAC 
TCGCGGCTAC GGCGGGGGGG CAGCACTGAC CAACCAACTG ACAGGTCCAG GTCTTCTGGG 
ACGGACCACA CAGGAGGCCA GCGGGCCCAG GGGACACTGA TGGAACATGC GCTGCTAAAT 
GACGAGAAGA TGCTGAAGGA AATCCAGAGC ACGCTGGAAG GAACCCGAAT AAGCTACAGC 
GAAAGGAGTT CCCGGCAGAT GAGCGTTGGA CACAGACTGA TCAATGGGGC AGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

aPKC, Crumbs3 and Lgl2 control apicobasal polarity in early vertebrate development.
Development (Cambridge, England)132(5)977-86(2005 Mar)
Chalmers AD,Pambos M,Mason J,Lang S,Wylie C,Papalopulu N

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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llgl2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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