grhl2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	grhl2
Entrez Gene ID	496802
Full Name	grainyhead like transcription factor 2
Synonyms	bom,dfna28,tfcp2l3
General protein information	Preferred Names grainyhead like transcription factor 2 Names grainyhead-like protein 2 homolog grainyhead 2 grainyhead-like 2 transcription factor CP2-like 3
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012964616.2	XP_012820070.1	grainyhead-like protein 2 homolog isoform X1
XM_031903346.1	XP_031759206.1	grainyhead-like protein 2 homolog isoform X1
XM_012964617.2	XP_012820071.1	grainyhead-like protein 2 homolog isoform X2
XM_012964618.2	XP_012820072.1	grainyhead-like protein 2 homolog isoform X3
XM_018094634.1	XP_017950123.1	grainyhead-like protein 2 homolog isoform X4
XM_031903347.1	XP_031759207.1	grainyhead-like protein 2 homolog isoform X2
NM_001011338.1	NP_001011338.1	grainyhead-like protein 2 homolog

Pathways from BioSystems

Homologies

Homo sapiens (human)	GRHL2	NP_079191.2
	GRHL2	XP_001098439.2
Canis lupus familiaris (dog)	GRHL2	XP_005627890.1
Gallus gallus (chicken)	GRHL2	XP_004940038.1
Xenopus tropicalis (tropical clawed frog)	grhl2	NP_001011338.1
Pan troglodytes (chimpanzee)	GRHL2	XP_519890.3
Mus musculus (house mouse)	Grhl2	NP_080772.2
Bos taurus (cattle)	GRHL2	XP_002692840.1
Rattus norvegicus (Norway rat)	Grhl2	NP_001127999.1
Danio rerio (zebrafish)	grhl2b	NP_001076541.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following grhl2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the grhl2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa00220	NM_001011338.1 Latest version!	Xenopus tropicalis grainyhead like transcription factor 2 (grhl2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa27227	XM_012964616.2 Latest version!	Xenopus tropicalis grainyhead-like transcription factor 2 (grhl2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa27228	XM_012964617.2 Latest version!	Xenopus tropicalis grainyhead-like transcription factor 2 (grhl2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa27229	XM_012964618.2 Latest version!	Xenopus tropicalis grainyhead-like transcription factor 2 (grhl2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa44914	XM_018094634.1 Latest version!	Xenopus tropicalis grainyhead-like transcription factor 2 (grhl2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OXa59652	XM_031903346.1 Latest version!	Xenopus tropicalis grainyhead like transcription factor 2 (grhl2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa59653	XM_031903347.1 Latest version!	Xenopus tropicalis grainyhead like transcription factor 2 (grhl2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

grhl2 ( NM_001011338.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001011338.1 Xenopus tropicalis grainyhead like transcription factor 2 (grhl2), mRNA.

Price & Availability↑

CloneID	OXa00220
Clone ID Related Accession (Same CDS sequence)	NM_001011338.1
Accession Version	NM_001011338.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Xenopus tropicalis(tropical clawed frog)
Product	grainyhead-like protein 2 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC088489.1. ##Evidence-Data-START## Transcript exon combination :: BC088489.1, CR855659.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGTCACAAG AGACGGACAA TAAACGACTT GTGGTCTTGG TTCCAAATGA CGCTGTGTTT 
AACCCTCGCC GTGCCTACAC CAGTGAAGAT GAGGCCTGGA AATCATACCT GGAGAATCCC 
TTAACGGCCG CAACAAAGGC AATGATGAGT ATAAATGGGG ATGAGGAAAG TGCAGCGGCC 
ATTGGACTAT TGTATGATTA TTACAAGGTT CCAAGAGAGA AAAGGTTGTT ATCGTTAAAT 
AAAATAAACG AAGGCCATGA AGATCAGGAT AAAAGAAATT GCCTTCCAGC CAATGAAACA 
CCAAGCAACT TGAGCACAGG AGAAAATCGT GTGCAAGTTC TGAAAACGGT TCCTGTCAAC 
CTGTCGCTCA ACAATGATAC TGTTGAGAGT TCCAACAGAG AGAAATACAC CACCAGCCTG 
TCTGAAAGCC CCCAGCCTGC TCCTGCGTCG GCGGTCACCG TGGTGAAAGC AGAGGAGTTC 
ACACCAGTGT TTATGGCCCC TTCAGTGCCT TATAGAAGCG ACGGAGAAGA ACCCCGAGGG 
GTAATCTTTG AGCAGACTCA CTTTGGAGTA CATAGTATTA CTACTCATTC GGACTACCTC 
AAGGATGACC AGCGCAGCAC TCCGGACAGC ACTTACAATG AAAGTTTTAA AGAAACCACT 
GAGAAATACC GGACCCCTTC AGTAGGAACA GAGGAATTTC TTTATGAACA AACTGCAAGC 
AGCACCTTTC AGTATACATT AGAAGCCACA AAATCTCTGC GCCAGAAGCA AGGAGAGGGG 
CCAATGACCT ATTTAAACAA AGGACAATTT TATGCCATCA CTCTGAGCGA AACTGGAGCC 
AACAAGTGTT TCCGACATCC CATCAGCAAA GTCAGGAGTG TAATTATGGT CGTATTCAGT 
GAAGATAAAA ACCGAGACGA ACAGTTAAAA TATTGGAAAT ACTGGCACTC GCGACAGCAT 
ACAGCAAAGC AGCGGGTTCT GGACATTGCT GACTACAAAG AAAGCTTTAA CACTATAGGC 
AATATTGAAG AAATTGCTTA CAATGCGGTG TCTTTCACAT GGGATGTCAA TGAAGAAGCC 
AAGATTTTCA TCACCGTCAA TTGCCTGAGT ACTGATTTTT CCTCGCAGAA AGGGGTCAAA 
GGGCTGCCTC TTATGATTCA GATTGACACA TACAGTTACA ATAACCGCAG CAACAAGCCA 
ATCCACCGGG CATACTGCCA GATCAAGGTC TTTTGTGATA AGGGAGCGGA AAGAAAAATC 
CGTGACGAGG AACGGAAACA GAACAGAAAG AAAGGCAAGA GCCAGGCGGC ACAGGCTCAG 
TGCAACAATT CAGCCGACGG CAAGTTATCA GCAGTGCCCT TGCAGAAGAA AAGTGACATT 
ACCTTCTTCA AAACAATGAC CGACTTGGAT GTGCAGCCTG TTCTCTTTAT TCCTGATGTC 
CACTTTGGGA ACCTTCAAAG GACCGGCCAG GTTTTCTACA ACACCGATGA TGACATTGAA 
GGTGGCGTCT TGGTGAAACG GTTGCTTCGC CCTGTGGATG AAGACTATGG CCCTCCTGCA 
CCCAAGCAAA TGAAAGAAGG AAGCAGGAAA GTGCTTCTAT ACGTAAGGAA AGAAACTGAT 
GAAGTATTCG ACGCCTTGAT GCTGAAGTAC CCAACTGTTA AAGGGCTCCT CGAGGCTATA 
TCTGAAAAAT ATGGGATCCC CGTGGAAAAG ATTGTGAAGA TTTATAAGAA GAGCAAAAAA 
GGGATCTTGG TCAATATGGA TGACAACATC ATTGAACACT ACTCCAATGA GGACACATTC 
ATTCTAAATG TGGAAAGCTT GGCGGAACAA GGCTACAAGA TCACCCTGAC AGAAATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001011338.1
CDS	131..1990
Translation	MSQETDNKRLVVLVPNDAVFNPRRAYTSEDEAWKSYLENPLTAATKAMMSINGDEESAAAIGLLYDYYKVPREKRLLSLNKINEGHEDQDKRNCLPANETPSNLSTGENRVQVLKTVPVNLSLNNDTVESSNREKYTTSLSESPQPAPASAVTVVKAEEFTPVFMAPSVPYRSDGEEPRGVIFEQTHFGVHSITTHSDYLKDDQRSTPDSTYNESFKETTEKYRTPSVGTEEFLYEQTASSTFQYTLEATKSLRQKQGEGPMTYLNKGQFYAITLSETGANKCFRHPISKVRSVIMVVFSEDKNRDEQLKYWKYWHSRQHTAKQRVLDIADYKESFNTIGNIEEIAYNAVSFTWDVNEEAKIFITVNCLSTDFSSQKGVKGLPLMIQIDTYSYNNRSNKPIHRAYCQIKVFCDKGAERKIRDEERKQNRKKGKSQAAQAQCNNSADGKLSAVPLQKKSDITFFKTMTDLDVQPVLFIPDVHFGNLQRTGQVFYNTDDDIEGGVLVKRLLRPVDEDYGPPAPKQMKEGSRKVLLYVRKETDEVFDALMLKYPTVKGLLEAISEKYGIPVEKIVKIYKKSKKGILVNMDDNIIEHYSNEDTFILNVESLAEQGYKITLTEI

Target ORF information:

RefSeq Version	NM_001011338.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis grainyhead like transcription factor 2 (grhl2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001011338.1

ORF Insert Sequence:

ATGTCACAAG AGACGGACAA TAAACGACTT GTGGTCTTGG TTCCAAATGA CGCTGTGTTT 
AACCCTCGCC GTGCCTACAC CAGTGAAGAT GAGGCCTGGA AATCATACCT GGAGAATCCC 
TTAACGGCCG CAACAAAGGC AATGATGAGT ATAAATGGGG ATGAGGAAAG TGCAGCGGCC 
ATTGGACTAT TGTATGATTA TTACAAGGTT CCAAGAGAGA AAAGGTTGTT ATCGTTAAAT 
AAAATAAACG AAGGCCATGA AGATCAGGAT AAAAGAAATT GCCTTCCAGC CAATGAAACA 
CCAAGCAACT TGAGCACAGG AGAAAATCGT GTGCAAGTTC TGAAAACGGT TCCTGTCAAC 
CTGTCGCTCA ACAATGATAC TGTTGAGAGT TCCAACAGAG AGAAATACAC CACCAGCCTG 
TCTGAAAGCC CCCAGCCTGC TCCTGCGTCG GCGGTCACCG TGGTGAAAGC AGAGGAGTTC 
ACACCAGTGT TTATGGCCCC TTCAGTGCCT TATAGAAGCG ACGGAGAAGA ACCCCGAGGG 
GTAATCTTTG AGCAGACTCA CTTTGGAGTA CATAGTATTA CTACTCATTC GGACTACCTC 
AAGGATGACC AGCGCAGCAC TCCGGACAGC ACTTACAATG AAAGTTTTAA AGAAACCACT 
GAGAAATACC GGACCCCTTC AGTAGGAACA GAGGAATTTC TTTATGAACA AACTGCAAGC 
AGCACCTTTC AGTATACATT AGAAGCCACA AAATCTCTGC GCCAGAAGCA AGGAGAGGGG 
CCAATGACCT ATTTAAACAA AGGACAATTT TATGCCATCA CTCTGAGCGA AACTGGAGCC 
AACAAGTGTT TCCGACATCC CATCAGCAAA GTCAGGAGTG TAATTATGGT CGTATTCAGT 
GAAGATAAAA ACCGAGACGA ACAGTTAAAA TATTGGAAAT ACTGGCACTC GCGACAGCAT 
ACAGCAAAGC AGCGGGTTCT GGACATTGCT GACTACAAAG AAAGCTTTAA CACTATAGGC 
AATATTGAAG AAATTGCTTA CAATGCGGTG TCTTTCACAT GGGATGTCAA TGAAGAAGCC 
AAGATTTTCA TCACCGTCAA TTGCCTGAGT ACTGATTTTT CCTCGCAGAA AGGGGTCAAA 
GGGCTGCCTC TTATGATTCA GATTGACACA TACAGTTACA ATAACCGCAG CAACAAGCCA 
ATCCACCGGG CATACTGCCA GATCAAGGTC TTTTGTGATA AGGGAGCGGA AAGAAAAATC 
CGTGACGAGG AACGGAAACA GAACAGAAAG AAAGGCAAGA GCCAGGCGGC ACAGGCTCAG 
TGCAACAATT CAGCCGACGG CAAGTTATCA GCAGTGCCCT TGCAGAAGAA AAGTGACATT 
ACCTTCTTCA AAACAATGAC CGACTTGGAT GTGCAGCCTG TTCTCTTTAT TCCTGATGTC 
CACTTTGGGA ACCTTCAAAG GACCGGCCAG GTTTTCTACA ACACCGATGA TGACATTGAA 
GGTGGCGTCT TGGTGAAACG GTTGCTTCGC CCTGTGGATG AAGACTATGG CCCTCCTGCA 
CCCAAGCAAA TGAAAGAAGG AAGCAGGAAA GTGCTTCTAT ACGTAAGGAA AGAAACTGAT 
GAAGTATTCG ACGCCTTGAT GCTGAAGTAC CCAACTGTTA AAGGGCTCCT CGAGGCTATA 
TCTGAAAAAT ATGGGATCCC CGTGGAAAAG ATTGTGAAGA TTTATAAGAA GAGCAAAAAA 
GGGATCTTGG TCAATATGGA TGACAACATC ATTGAACACT ACTCCAATGA GGACACATTC 
ATTCTAAATG TGGAAAGCTT GGCGGAACAA GGCTACAAGA TCACCCTGAC AGAAATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grhl2 ( XM_012964616.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820070.1 Xenopus tropicalis grainyhead-like transcription factor 2 (grhl2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa27227
Clone ID Related Accession (Same CDS sequence)	XM_012964616.2
Accession Version	XM_012964616.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	grainyhead-like protein 2 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964616.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCA CGTCCACTGC ATACAGTAAA CGACTTGTGG TCTTGGTTCC AAATGACGCT 
GTGTTTAACC CTCGCCGTGC CTACACCAGT GAAGATGAGG CCTGGAAATC ATACCTGGAG 
AATCCCTTAA CGGCCGCAAC AAAGGCAATG ATGAGTATAA ATGGGGATGA GGAAAGTGCA 
GCGGCCATTG GACTATTGTA TGATTATTAC AAGGTTCCAA GAGAGAAAAG GTTGTTATCG 
TTAAATAAAA TAAACGAAGG CCATGAAGAT CAGGATAAAA GAAATTGCCT TCCAGCCAAT 
GAAACACCAA GCAACTTGAG CACAGGAGAA AATCGTGTGC AAGTTCTGAA AACGGTTCCT 
GTCAACCTGT CGCTCAACAA TGATACTGTT GAGAGTTCCA ACAGAGAGAA ATACACCACC 
AGCCTGTCTG AAAGCCCCCA GCCGGCTCCT GCGTCGGCGG TCACTGTGGT GAAAGCAGAG 
GAGTTCACAC CAGTGTTTAT GGCCCCTTCA GTGCCTTATA GAAGCGACGG AGAAGAACCC 
CGAGGGGTAA TCTTTGAGCA GACTCACTTT GGAGTACATA GTATTACTAC TCATTCGGAC 
TACCTCAAGG ATGACCAGCG CAGCACTCCG GACAGCACTT ACAATGAAAG TTTTAAAGAA 
ACCACTGAGA AATACCGGAC CCCTTCAGTA GGAACAGAGG AATTTCTTTA TGAACAAACT 
GCAAGCAGCA CCTTTCAGTA TACATTAGAA GCCACAAAAT CTCTGCGCCA GAAGCAAGGA 
GAGGGGCCAA TGACCTATTT AAACAAAGGA CAATTTTATG CCATCACTCT GAGCGAAACT 
GGAGCCAACA AGTGTTTCCG ACATCCCATC AGCAAAGTCA GGAGTGTAAT TATGGTCGTA 
TTCAGTGAAG ATAAAAACCG AGACGAACAG TTAAAATATT GGAAATACTG GCACTCGCGA 
CAGCATACAG CAAAGCAGCG GGTTCTGGAC ATTGCTGACT ACAAAGAAAG CTTTAACACT 
ATAGGCAATA TTGAAGAAAT TGCTTACAAT GCGGTGTCTT TCACATGGGA TGTCAATGAA 
GAAGCCAAGA TTTTCATCAC CGTCAATTGC CTGAGTACTG ATTTTTCCTC GCAGAAAGGG 
GTCAAAGGGC TGCCTCTTAT GATTCAGATT GACACATACA GTTACAATAA CCGCAGCAAC 
AAGCCAATCC ACCGGGCATA CTGCCAGATC AAGGTCTTTT GTGATAAGGG AGCGGAAAGA 
AAAATCCGTG ACGAGGAACG TAAACAGAAC AGAAAGAAAG GCAAGAGCCA GGCGGCACAG 
GCTCAGTGCA ACAATTCAGC CGACGGCAAG TTATCAGCAG TGCCCTTGCA GAAGAAAAGT 
GACATTACCT TCTTCAAAAC AATGACCGAC TTGGATGTGC AGCCTGTTCT CTTTATTCCT 
GATGTCCACT TTGGGAACCT TCAAAGGACC GGCCAGGTTT TCTACAACAC CGATGATGAC 
ATTGAAGGTG GCGTCTTGGT GAAACGGTTG CTTCGCCCTG TGGATGAAGA CTATGGCCCT 
CCTGCACCCA AGCAAATGAA AGAAGGAAGC AGGAAAGTGC TTCTATACGT AAGGAAAGAA 
ACTGATGAAG TATTCGACGC CTTGATGCTG AAGTACCCAA CTGTTAAAGG GCTCCTCGAG 
GCTATATCTG AAAAATATGG GATCCCCGTG GAAAAGATTG TGAAGATTTA TAAGAAGAGC 
AAAAAAGGGA TCTTGGTCAA TATGGATGAC AACATCATTG AACACTACTC CAATGAGGAC 
ACATTCATTC TAAATGTGGA AAGCTTGGCG GAACAAGGCT ACAAGATCAC CCTGACAGAA 
ATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820070.1
CDS	1047..2912
Translation	MLGTSTAYSKRLVVLVPNDAVFNPRRAYTSEDEAWKSYLENPLTAATKAMMSINGDEESAAAIGLLYDYYKVPREKRLLSLNKINEGHEDQDKRNCLPANETPSNLSTGENRVQVLKTVPVNLSLNNDTVESSNREKYTTSLSESPQPAPASAVTVVKAEEFTPVFMAPSVPYRSDGEEPRGVIFEQTHFGVHSITTHSDYLKDDQRSTPDSTYNESFKETTEKYRTPSVGTEEFLYEQTASSTFQYTLEATKSLRQKQGEGPMTYLNKGQFYAITLSETGANKCFRHPISKVRSVIMVVFSEDKNRDEQLKYWKYWHSRQHTAKQRVLDIADYKESFNTIGNIEEIAYNAVSFTWDVNEEAKIFITVNCLSTDFSSQKGVKGLPLMIQIDTYSYNNRSNKPIHRAYCQIKVFCDKGAERKIRDEERKQNRKKGKSQAAQAQCNNSADGKLSAVPLQKKSDITFFKTMTDLDVQPVLFIPDVHFGNLQRTGQVFYNTDDDIEGGVLVKRLLRPVDEDYGPPAPKQMKEGSRKVLLYVRKETDEVFDALMLKYPTVKGLLEAISEKYGIPVEKIVKIYKKSKKGILVNMDDNIIEHYSNEDTFILNVESLAEQGYKITLTEI

Target ORF information:

RefSeq Version	XM_012964616.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis grainyhead-like transcription factor 2 (grhl2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964616.2

ORF Insert Sequence:

ATGTTGGGCA CGTCCACTGC ATACAGTAAA CGACTTGTGG TCTTGGTTCC AAATGACGCT 
GTGTTTAACC CTCGCCGTGC CTACACCAGT GAAGATGAGG CCTGGAAATC ATACCTGGAG 
AATCCCTTAA CGGCCGCAAC AAAGGCAATG ATGAGTATAA ATGGGGATGA GGAAAGTGCA 
GCGGCCATTG GACTATTGTA TGATTATTAC AAGGTTCCAA GAGAGAAAAG GTTGTTATCG 
TTAAATAAAA TAAACGAAGG CCATGAAGAT CAGGATAAAA GAAATTGCCT TCCAGCCAAT 
GAAACACCAA GCAACTTGAG CACAGGAGAA AATCGTGTGC AAGTTCTGAA AACGGTTCCT 
GTCAACCTGT CGCTCAACAA TGATACTGTT GAGAGTTCCA ACAGAGAGAA ATACACCACC 
AGCCTGTCTG AAAGCCCCCA GCCGGCTCCT GCGTCGGCGG TCACTGTGGT GAAAGCAGAG 
GAGTTCACAC CAGTGTTTAT GGCCCCTTCA GTGCCTTATA GAAGCGACGG AGAAGAACCC 
CGAGGGGTAA TCTTTGAGCA GACTCACTTT GGAGTACATA GTATTACTAC TCATTCGGAC 
TACCTCAAGG ATGACCAGCG CAGCACTCCG GACAGCACTT ACAATGAAAG TTTTAAAGAA 
ACCACTGAGA AATACCGGAC CCCTTCAGTA GGAACAGAGG AATTTCTTTA TGAACAAACT 
GCAAGCAGCA CCTTTCAGTA TACATTAGAA GCCACAAAAT CTCTGCGCCA GAAGCAAGGA 
GAGGGGCCAA TGACCTATTT AAACAAAGGA CAATTTTATG CCATCACTCT GAGCGAAACT 
GGAGCCAACA AGTGTTTCCG ACATCCCATC AGCAAAGTCA GGAGTGTAAT TATGGTCGTA 
TTCAGTGAAG ATAAAAACCG AGACGAACAG TTAAAATATT GGAAATACTG GCACTCGCGA 
CAGCATACAG CAAAGCAGCG GGTTCTGGAC ATTGCTGACT ACAAAGAAAG CTTTAACACT 
ATAGGCAATA TTGAAGAAAT TGCTTACAAT GCGGTGTCTT TCACATGGGA TGTCAATGAA 
GAAGCCAAGA TTTTCATCAC CGTCAATTGC CTGAGTACTG ATTTTTCCTC GCAGAAAGGG 
GTCAAAGGGC TGCCTCTTAT GATTCAGATT GACACATACA GTTACAATAA CCGCAGCAAC 
AAGCCAATCC ACCGGGCATA CTGCCAGATC AAGGTCTTTT GTGATAAGGG AGCGGAAAGA 
AAAATCCGTG ACGAGGAACG TAAACAGAAC AGAAAGAAAG GCAAGAGCCA GGCGGCACAG 
GCTCAGTGCA ACAATTCAGC CGACGGCAAG TTATCAGCAG TGCCCTTGCA GAAGAAAAGT 
GACATTACCT TCTTCAAAAC AATGACCGAC TTGGATGTGC AGCCTGTTCT CTTTATTCCT 
GATGTCCACT TTGGGAACCT TCAAAGGACC GGCCAGGTTT TCTACAACAC CGATGATGAC 
ATTGAAGGTG GCGTCTTGGT GAAACGGTTG CTTCGCCCTG TGGATGAAGA CTATGGCCCT 
CCTGCACCCA AGCAAATGAA AGAAGGAAGC AGGAAAGTGC TTCTATACGT AAGGAAAGAA 
ACTGATGAAG TATTCGACGC CTTGATGCTG AAGTACCCAA CTGTTAAAGG GCTCCTCGAG 
GCTATATCTG AAAAATATGG GATCCCCGTG GAAAAGATTG TGAAGATTTA TAAGAAGAGC 
AAAAAAGGGA TCTTGGTCAA TATGGATGAC AACATCATTG AACACTACTC CAATGAGGAC 
ACATTCATTC TAAATGTGGA AAGCTTGGCG GAACAAGGCT ACAAGATCAC CCTGACAGAA 
ATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grhl2 ( XM_012964617.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820071.1 Xenopus tropicalis grainyhead-like transcription factor 2 (grhl2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa27228
Clone ID Related Accession (Same CDS sequence)	XM_012964617.2
Accession Version	XM_012964617.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	grainyhead-like protein 2 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964617.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCA CGTCCACTGC ATACAGTAAA CGACTTGTGG TCTTGGTTCC AAATGACGCT 
GTGTTTAACC CTCGCCGTGC CTACACCAGT GAAGATGAGG CCTGGAAATC ATACCTGGAG 
AATCCCTTAA CGGCCGCAAC AAAGGCAATG ATGAGTATAA ATGGGGATGA GGAAAGTGCA 
GCGGCCATTG GACTATTGTA TGATTATTAC AAGGTTCCAA GAGAGAAAAG GTTGTTATCG 
TTAAATAAAA TAAACGAAGG CCATGAAGAT CAGGATAAAA GAAATTGCCT TCCAGCCAAT 
GAAACACCAA GCAACTTGAG CACAGGAGAA AATCGTGTGC AAGTTCTGAA AACGGTTCCT 
GTCAACCTGT CGCTCAACAA TGATACTGTT GAGAGTTCCA ACAGAGAGAA ATACACCACC 
AGCCTGTCTG AAAGCCCCCA GCCGGCTCCT GCGTCGGCGG TCACTGTGGT GAAAGCAGAG 
GAGTTCACAC CAGTGTTTAT GGCCCCTTCA GTGCCTTATA GAAGCGACGG AGAAGAACCC 
CGAGGGGTAA TCTTTGAGCA GACTCACTTT GGAGTACATA GTATTACTAC TCATTCGGAC 
TACCTCAAGG ATGACCAGCG CAGCACTCCG GACAGCACTT ACAATGAAAG TTTTAAAGAA 
ACCACTGAGA AATACCGGAC CCCTTCAGTA GGAACAGAGG AATTTCTTTA TGAACAAACT 
GCAAGCACCT TTCAGTATAC ATTAGAAGCC ACAAAATCTC TGCGCCAGAA GCAAGGAGAG 
GGGCCAATGA CCTATTTAAA CAAAGGACAA TTTTATGCCA TCACTCTGAG CGAAACTGGA 
GCCAACAAGT GTTTCCGACA TCCCATCAGC AAAGTCAGGA GTGTAATTAT GGTCGTATTC 
AGTGAAGATA AAAACCGAGA CGAACAGTTA AAATATTGGA AATACTGGCA CTCGCGACAG 
CATACAGCAA AGCAGCGGGT TCTGGACATT GCTGACTACA AAGAAAGCTT TAACACTATA 
GGCAATATTG AAGAAATTGC TTACAATGCG GTGTCTTTCA CATGGGATGT CAATGAAGAA 
GCCAAGATTT TCATCACCGT CAATTGCCTG AGTACTGATT TTTCCTCGCA GAAAGGGGTC 
AAAGGGCTGC CTCTTATGAT TCAGATTGAC ACATACAGTT ACAATAACCG CAGCAACAAG 
CCAATCCACC GGGCATACTG CCAGATCAAG GTCTTTTGTG ATAAGGGAGC GGAAAGAAAA 
ATCCGTGACG AGGAACGTAA ACAGAACAGA AAGAAAGGCA AGAGCCAGGC GGCACAGGCT 
CAGTGCAACA ATTCAGCCGA CGGCAAGTTA TCAGCAGTGC CCTTGCAGAA GAAAAGTGAC 
ATTACCTTCT TCAAAACAAT GACCGACTTG GATGTGCAGC CTGTTCTCTT TATTCCTGAT 
GTCCACTTTG GGAACCTTCA AAGGACCGGC CAGGTTTTCT ACAACACCGA TGATGACATT 
GAAGGTGGCG TCTTGGTGAA ACGGTTGCTT CGCCCTGTGG ATGAAGACTA TGGCCCTCCT 
GCACCCAAGC AAATGAAAGA AGGAAGCAGG AAAGTGCTTC TATACGTAAG GAAAGAAACT 
GATGAAGTAT TCGACGCCTT GATGCTGAAG TACCCAACTG TTAAAGGGCT CCTCGAGGCT 
ATATCTGAAA AATATGGGAT CCCCGTGGAA AAGATTGTGA AGATTTATAA GAAGAGCAAA 
AAAGGGATCT TGGTCAATAT GGATGACAAC ATCATTGAAC ACTACTCCAA TGAGGACACA 
TTCATTCTAA ATGTGGAAAG CTTGGCGGAA CAAGGCTACA AGATCACCCT GACAGAAATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820071.1
CDS	1047..2909
Translation	MLGTSTAYSKRLVVLVPNDAVFNPRRAYTSEDEAWKSYLENPLTAATKAMMSINGDEESAAAIGLLYDYYKVPREKRLLSLNKINEGHEDQDKRNCLPANETPSNLSTGENRVQVLKTVPVNLSLNNDTVESSNREKYTTSLSESPQPAPASAVTVVKAEEFTPVFMAPSVPYRSDGEEPRGVIFEQTHFGVHSITTHSDYLKDDQRSTPDSTYNESFKETTEKYRTPSVGTEEFLYEQTASTFQYTLEATKSLRQKQGEGPMTYLNKGQFYAITLSETGANKCFRHPISKVRSVIMVVFSEDKNRDEQLKYWKYWHSRQHTAKQRVLDIADYKESFNTIGNIEEIAYNAVSFTWDVNEEAKIFITVNCLSTDFSSQKGVKGLPLMIQIDTYSYNNRSNKPIHRAYCQIKVFCDKGAERKIRDEERKQNRKKGKSQAAQAQCNNSADGKLSAVPLQKKSDITFFKTMTDLDVQPVLFIPDVHFGNLQRTGQVFYNTDDDIEGGVLVKRLLRPVDEDYGPPAPKQMKEGSRKVLLYVRKETDEVFDALMLKYPTVKGLLEAISEKYGIPVEKIVKIYKKSKKGILVNMDDNIIEHYSNEDTFILNVESLAEQGYKITLTEI

Target ORF information:

RefSeq Version	XM_012964617.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis grainyhead-like transcription factor 2 (grhl2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964617.2

ORF Insert Sequence:

ATGTTGGGCA CGTCCACTGC ATACAGTAAA CGACTTGTGG TCTTGGTTCC AAATGACGCT 
GTGTTTAACC CTCGCCGTGC CTACACCAGT GAAGATGAGG CCTGGAAATC ATACCTGGAG 
AATCCCTTAA CGGCCGCAAC AAAGGCAATG ATGAGTATAA ATGGGGATGA GGAAAGTGCA 
GCGGCCATTG GACTATTGTA TGATTATTAC AAGGTTCCAA GAGAGAAAAG GTTGTTATCG 
TTAAATAAAA TAAACGAAGG CCATGAAGAT CAGGATAAAA GAAATTGCCT TCCAGCCAAT 
GAAACACCAA GCAACTTGAG CACAGGAGAA AATCGTGTGC AAGTTCTGAA AACGGTTCCT 
GTCAACCTGT CGCTCAACAA TGATACTGTT GAGAGTTCCA ACAGAGAGAA ATACACCACC 
AGCCTGTCTG AAAGCCCCCA GCCGGCTCCT GCGTCGGCGG TCACTGTGGT GAAAGCAGAG 
GAGTTCACAC CAGTGTTTAT GGCCCCTTCA GTGCCTTATA GAAGCGACGG AGAAGAACCC 
CGAGGGGTAA TCTTTGAGCA GACTCACTTT GGAGTACATA GTATTACTAC TCATTCGGAC 
TACCTCAAGG ATGACCAGCG CAGCACTCCG GACAGCACTT ACAATGAAAG TTTTAAAGAA 
ACCACTGAGA AATACCGGAC CCCTTCAGTA GGAACAGAGG AATTTCTTTA TGAACAAACT 
GCAAGCACCT TTCAGTATAC ATTAGAAGCC ACAAAATCTC TGCGCCAGAA GCAAGGAGAG 
GGGCCAATGA CCTATTTAAA CAAAGGACAA TTTTATGCCA TCACTCTGAG CGAAACTGGA 
GCCAACAAGT GTTTCCGACA TCCCATCAGC AAAGTCAGGA GTGTAATTAT GGTCGTATTC 
AGTGAAGATA AAAACCGAGA CGAACAGTTA AAATATTGGA AATACTGGCA CTCGCGACAG 
CATACAGCAA AGCAGCGGGT TCTGGACATT GCTGACTACA AAGAAAGCTT TAACACTATA 
GGCAATATTG AAGAAATTGC TTACAATGCG GTGTCTTTCA CATGGGATGT CAATGAAGAA 
GCCAAGATTT TCATCACCGT CAATTGCCTG AGTACTGATT TTTCCTCGCA GAAAGGGGTC 
AAAGGGCTGC CTCTTATGAT TCAGATTGAC ACATACAGTT ACAATAACCG CAGCAACAAG 
CCAATCCACC GGGCATACTG CCAGATCAAG GTCTTTTGTG ATAAGGGAGC GGAAAGAAAA 
ATCCGTGACG AGGAACGTAA ACAGAACAGA AAGAAAGGCA AGAGCCAGGC GGCACAGGCT 
CAGTGCAACA ATTCAGCCGA CGGCAAGTTA TCAGCAGTGC CCTTGCAGAA GAAAAGTGAC 
ATTACCTTCT TCAAAACAAT GACCGACTTG GATGTGCAGC CTGTTCTCTT TATTCCTGAT 
GTCCACTTTG GGAACCTTCA AAGGACCGGC CAGGTTTTCT ACAACACCGA TGATGACATT 
GAAGGTGGCG TCTTGGTGAA ACGGTTGCTT CGCCCTGTGG ATGAAGACTA TGGCCCTCCT 
GCACCCAAGC AAATGAAAGA AGGAAGCAGG AAAGTGCTTC TATACGTAAG GAAAGAAACT 
GATGAAGTAT TCGACGCCTT GATGCTGAAG TACCCAACTG TTAAAGGGCT CCTCGAGGCT 
ATATCTGAAA AATATGGGAT CCCCGTGGAA AAGATTGTGA AGATTTATAA GAAGAGCAAA 
AAAGGGATCT TGGTCAATAT GGATGACAAC ATCATTGAAC ACTACTCCAA TGAGGACACA 
TTCATTCTAA ATGTGGAAAG CTTGGCGGAA CAAGGCTACA AGATCACCCT GACAGAAATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grhl2 ( XM_012964618.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820072.1 Xenopus tropicalis grainyhead-like transcription factor 2 (grhl2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa27229
Clone ID Related Accession (Same CDS sequence)	XM_012964618.2
Accession Version	XM_012964618.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	grainyhead-like protein 2 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964618.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCA CGTCCACTGC ATACAGTAAA CGACTTGTGG TCTTGGTTCC AAATGACGCT 
GTGTTTAACC CTCGCCGTGC CTACACCAGT GAAGATGAGG CCTGGAAATC ATACCTGGAG 
AATCCCTTAA CGGCCGCAAC AAAGGCAATG ATGAGTATAA ATGGGGATGA GGAAAGTGCA 
GCGGCCATTG GACTATTGTA TGATTATTAC AAGGTTCCAA GAGAGAAAAG GTTGTTATCG 
TTAAATAAAA TAAACGAAGG CCATGAAGAT CAGGATAAAA GAAATTGCCT TCCAGCCAAT 
GAAACACCAA GCAACTTGAG CACAGGAGAA AATCGTGTGC AAGTTCTGAA AACGGTTCCT 
GTCAACCTGT CGCTCAACAA TGATACTGTT GAGAGTTCCA ACAGAGAGAA ATACACCACC 
AGCCTGTCTG AAAGCCCCCA GCCGGCTCCT GCGTCGGCGG TCACTGTGGT GAAAGCAGAG 
GAGTTCACAC CAGTGTTTAT GGCCCCTTCA GTGCCTTATA GAAGCGACGG AGAAGAACCC 
CGAGGGGTAA TCTTTGAGCA GACTCACTTT GGAGTACATA GTATTACTAC TCATTCGGAC 
TACCTCAAGG ATGACCAGCG CAGCACTCCG GACAGCACTT ACAATGAAAG TTTTAAAGAA 
ACCACTGAGA AATACCGGAC CCCTTCAGTA GGAACAGAGG AATTTCTTTA TGAACAAACT 
GCAAGCAGCA CCTTTCAGTA TACATTAGAA GCCACAAAAT CTCTGCGCCA GAAGCAAGGA 
GAGGGGCCAA TGACCTATTT AAACAAAGGA CAATTTTATG CCATCACTCT GAGCGAAACT 
GGAGCCAACA AGTGTTTCCG ACATCCCATC AGCAAAGTCA GGAGTGTAAT TATGGTCGTA 
TTCAGTGAAG ATAAAAACCG AGACGAACAG TTAAAATATT GGAAATACTG GCACTCGCGA 
CAGCATACAG CAAAGCAGCG GGTTCTGGAC ATTGCTGACT ACAAAGAAAG CTTTAACACT 
ATAGGCAATA TTGAAGAAAT TGCTTACAAT GCGGTGTCTT TCACATGGGA TGTCAATGAA 
GAAGCCAAGA TTTTCATCAC CGTCAATTGC CTGAGTACTG ATTTTTCCTC GCAGAAAGGG 
GTCAAAGGGC TGCCTCTTAT GATTCAGATT GACACATACA GTTACAATAA CCGCAGCAAC 
AAGCCAATCC ACCGGGCATA CTGCCAGATC AAGGTCTTTT GTGATAAGGG AGCGGAAAGA 
AAAATCCGTG ACGAGGAACG TAAACAGAAC AGAAAGAAAG GCAAGAGCCA GGCGGCACAG 
GCTCAGTGCA ACAATTCCGA CGGCAAGTTA TCAGCAGTGC CCTTGCAGAA GAAAAGTGAC 
ATTACCTTCT TCAAAACAAT GACCGACTTG GATGTGCAGC CTGTTCTCTT TATTCCTGAT 
GTCCACTTTG GGAACCTTCA AAGGACCGGC CAGGTTTTCT ACAACACCGA TGATGACATT 
GAAGGTGGCG TCTTGGTGAA ACGGTTGCTT CGCCCTGTGG ATGAAGACTA TGGCCCTCCT 
GCACCCAAGC AAATGAAAGA AGGAAGCAGG AAAGTGCTTC TATACGTAAG GAAAGAAACT 
GATGAAGTAT TCGACGCCTT GATGCTGAAG TACCCAACTG TTAAAGGGCT CCTCGAGGCT 
ATATCTGAAA AATATGGGAT CCCCGTGGAA AAGATTGTGA AGATTTATAA GAAGAGCAAA 
AAAGGGATCT TGGTCAATAT GGATGACAAC ATCATTGAAC ACTACTCCAA TGAGGACACA 
TTCATTCTAA ATGTGGAAAG CTTGGCGGAA CAAGGCTACA AGATCACCCT GACAGAAATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820072.1
CDS	1048..2910
Translation	MLGTSTAYSKRLVVLVPNDAVFNPRRAYTSEDEAWKSYLENPLTAATKAMMSINGDEESAAAIGLLYDYYKVPREKRLLSLNKINEGHEDQDKRNCLPANETPSNLSTGENRVQVLKTVPVNLSLNNDTVESSNREKYTTSLSESPQPAPASAVTVVKAEEFTPVFMAPSVPYRSDGEEPRGVIFEQTHFGVHSITTHSDYLKDDQRSTPDSTYNESFKETTEKYRTPSVGTEEFLYEQTASSTFQYTLEATKSLRQKQGEGPMTYLNKGQFYAITLSETGANKCFRHPISKVRSVIMVVFSEDKNRDEQLKYWKYWHSRQHTAKQRVLDIADYKESFNTIGNIEEIAYNAVSFTWDVNEEAKIFITVNCLSTDFSSQKGVKGLPLMIQIDTYSYNNRSNKPIHRAYCQIKVFCDKGAERKIRDEERKQNRKKGKSQAAQAQCNNSDGKLSAVPLQKKSDITFFKTMTDLDVQPVLFIPDVHFGNLQRTGQVFYNTDDDIEGGVLVKRLLRPVDEDYGPPAPKQMKEGSRKVLLYVRKETDEVFDALMLKYPTVKGLLEAISEKYGIPVEKIVKIYKKSKKGILVNMDDNIIEHYSNEDTFILNVESLAEQGYKITLTEI

Target ORF information:

RefSeq Version	XM_012964618.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis grainyhead-like transcription factor 2 (grhl2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964618.2

ORF Insert Sequence:

ATGTTGGGCA CGTCCACTGC ATACAGTAAA CGACTTGTGG TCTTGGTTCC AAATGACGCT 
GTGTTTAACC CTCGCCGTGC CTACACCAGT GAAGATGAGG CCTGGAAATC ATACCTGGAG 
AATCCCTTAA CGGCCGCAAC AAAGGCAATG ATGAGTATAA ATGGGGATGA GGAAAGTGCA 
GCGGCCATTG GACTATTGTA TGATTATTAC AAGGTTCCAA GAGAGAAAAG GTTGTTATCG 
TTAAATAAAA TAAACGAAGG CCATGAAGAT CAGGATAAAA GAAATTGCCT TCCAGCCAAT 
GAAACACCAA GCAACTTGAG CACAGGAGAA AATCGTGTGC AAGTTCTGAA AACGGTTCCT 
GTCAACCTGT CGCTCAACAA TGATACTGTT GAGAGTTCCA ACAGAGAGAA ATACACCACC 
AGCCTGTCTG AAAGCCCCCA GCCGGCTCCT GCGTCGGCGG TCACTGTGGT GAAAGCAGAG 
GAGTTCACAC CAGTGTTTAT GGCCCCTTCA GTGCCTTATA GAAGCGACGG AGAAGAACCC 
CGAGGGGTAA TCTTTGAGCA GACTCACTTT GGAGTACATA GTATTACTAC TCATTCGGAC 
TACCTCAAGG ATGACCAGCG CAGCACTCCG GACAGCACTT ACAATGAAAG TTTTAAAGAA 
ACCACTGAGA AATACCGGAC CCCTTCAGTA GGAACAGAGG AATTTCTTTA TGAACAAACT 
GCAAGCAGCA CCTTTCAGTA TACATTAGAA GCCACAAAAT CTCTGCGCCA GAAGCAAGGA 
GAGGGGCCAA TGACCTATTT AAACAAAGGA CAATTTTATG CCATCACTCT GAGCGAAACT 
GGAGCCAACA AGTGTTTCCG ACATCCCATC AGCAAAGTCA GGAGTGTAAT TATGGTCGTA 
TTCAGTGAAG ATAAAAACCG AGACGAACAG TTAAAATATT GGAAATACTG GCACTCGCGA 
CAGCATACAG CAAAGCAGCG GGTTCTGGAC ATTGCTGACT ACAAAGAAAG CTTTAACACT 
ATAGGCAATA TTGAAGAAAT TGCTTACAAT GCGGTGTCTT TCACATGGGA TGTCAATGAA 
GAAGCCAAGA TTTTCATCAC CGTCAATTGC CTGAGTACTG ATTTTTCCTC GCAGAAAGGG 
GTCAAAGGGC TGCCTCTTAT GATTCAGATT GACACATACA GTTACAATAA CCGCAGCAAC 
AAGCCAATCC ACCGGGCATA CTGCCAGATC AAGGTCTTTT GTGATAAGGG AGCGGAAAGA 
AAAATCCGTG ACGAGGAACG TAAACAGAAC AGAAAGAAAG GCAAGAGCCA GGCGGCACAG 
GCTCAGTGCA ACAATTCCGA CGGCAAGTTA TCAGCAGTGC CCTTGCAGAA GAAAAGTGAC 
ATTACCTTCT TCAAAACAAT GACCGACTTG GATGTGCAGC CTGTTCTCTT TATTCCTGAT 
GTCCACTTTG GGAACCTTCA AAGGACCGGC CAGGTTTTCT ACAACACCGA TGATGACATT 
GAAGGTGGCG TCTTGGTGAA ACGGTTGCTT CGCCCTGTGG ATGAAGACTA TGGCCCTCCT 
GCACCCAAGC AAATGAAAGA AGGAAGCAGG AAAGTGCTTC TATACGTAAG GAAAGAAACT 
GATGAAGTAT TCGACGCCTT GATGCTGAAG TACCCAACTG TTAAAGGGCT CCTCGAGGCT 
ATATCTGAAA AATATGGGAT CCCCGTGGAA AAGATTGTGA AGATTTATAA GAAGAGCAAA 
AAAGGGATCT TGGTCAATAT GGATGACAAC ATCATTGAAC ACTACTCCAA TGAGGACACA 
TTCATTCTAA ATGTGGAAAG CTTGGCGGAA CAAGGCTACA AGATCACCCT GACAGAAATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grhl2 ( XM_018094634.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950123.1 Xenopus tropicalis grainyhead-like transcription factor 2 (grhl2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa44914
Clone ID Related Accession (Same CDS sequence)	XM_018094634.1
Accession Version	XM_018094634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	grainyhead-like protein 2 homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCA CGTCCACTGC ATACAGTAAA CGACTTGTGG TCTTGGTTCC AAATGACGCT 
GTGTTTAACC CTCGCCGTGC CTACACCAGT GAAGATGAGG CCTGGAAATC ATACCTGGAG 
AATCCCTTAA CGGCCGCAAC AAAGGCAATG ATGAGTATAA ATGGGGATGA GGAAAGTGCA 
GCGGCCATTG GACTATTGTA TGATTATTAC AAGGTTCCAA GAGAGAAAAG GTTGTTATCG 
TTAAATAAAA TAAACGAAGG CCATGAAGAT CAGGATAAAA GAAATTGCCT TCCAGCCAAT 
GAAACACCAA GCAACTTGAG CACAGGAGAA AATCGTGTGC AAGTTCTGAA AACGGTTCCT 
GTCAACCTGT CGCTCAACAA TGATACTGTT GAGAGTTCCA ACAGAGAGAA ATACACCACC 
AGCCTGTCTG AAAGCCCCCA GCCGGCTCCT GCGTCGGCGG TCACTGTGGT GAAAGCAGAG 
GAGTTCACAC CAGTGTTTAT GGCCCCTTCA GTGCCTTATA GAAGCGACGG AGAAGAACCC 
CGAGGGGTAA TCTTTGAGCA GACTCACTTT GGAGTACATA GTATTACTAC TCATTCGGAC 
TACCTCAAGG ATGACCAGCG CAGCACTCCG GACAGCACTT ACAATGAAAG TTTTAAAGAA 
ACCACTGAGA AATACCGGAC CCCTTCAGTA GGAACAGAGG AATTTCTTTA TGAACAAACT 
GCAAGCAGCA CCTTTCAGTA TACATTAGAA GCCACAAAAT CTCTGCGCCA GAAGCAAGGA 
GAGGGGCCAA TGACCTATTT AAACAAAGGA CAATTTTATG CCATCACTCT GAGCGAAACT 
GGAGCCAACA AGTGTTTCCG ACATCCCATC AGCAAAGTCA GGATTTTCAT CACCGTCAAT 
TGCCTGAGTA CTGATTTTTC CTCGCAGAAA GGGGTCAAAG GGCTGCCTCT TATGATTCAG 
ATTGACACAT ACAGTTACAA TAACCGCAGC AACAAGCCAA TCCACCGGGC ATACTGCCAG 
ATCAAGGTCT TTTGTGATAA GGGAGCGGAA AGAAAAATCC GTGACGAGGA ACGTAAACAG 
AACAGAAAGA AAGGCAAGAG CCAGGCGGCA CAGGCTCAGT GCAACAATTC AGCCGACGGC 
AAGTTATCAG CAGTGCCCTT GCAGAAGAAA AGTGACATTA CCTTCTTCAA AACAATGACC 
GACTTGGATG TGCAGCCTGT TCTCTTTATT CCTGATGTCC ACTTTGGGAA CCTTCAAAGG 
ACCGGCCAGG TTTTCTACAA CACCGATGAT GACATTGAAG GTGGCGTCTT GGTGAAACGG 
TTGCTTCGCC CTGTGGATGA AGACTATGGC CCTCCTGCAC CCAAGCAAAT GAAAGAAGGA 
AGCAGGAAAG TGCTTCTATA CGTAAGGAAA GAAACTGATG AAGTATTCGA CGCCTTGATG 
CTGAAGTACC CAACTGTTAA AGGGCTCCTC GAGGCTATAT CTGAAAAATA TGGGATCCCC 
GTGGAAAAGA TTGTGAAGAT TTATAAGAAG AGCAAAAAAG GGATCTTGGT CAATATGGAT 
GACAACATCA TTGAACACTA CTCCAATGAG GACACATTCA TTCTAAATGT GGAAAGCTTG 
GCGGAACAAG GCTACAAGAT CACCCTGACA GAAATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950123.1
CDS	1047..2705
Translation	MLGTSTAYSKRLVVLVPNDAVFNPRRAYTSEDEAWKSYLENPLTAATKAMMSINGDEESAAAIGLLYDYYKVPREKRLLSLNKINEGHEDQDKRNCLPANETPSNLSTGENRVQVLKTVPVNLSLNNDTVESSNREKYTTSLSESPQPAPASAVTVVKAEEFTPVFMAPSVPYRSDGEEPRGVIFEQTHFGVHSITTHSDYLKDDQRSTPDSTYNESFKETTEKYRTPSVGTEEFLYEQTASSTFQYTLEATKSLRQKQGEGPMTYLNKGQFYAITLSETGANKCFRHPISKVRIFITVNCLSTDFSSQKGVKGLPLMIQIDTYSYNNRSNKPIHRAYCQIKVFCDKGAERKIRDEERKQNRKKGKSQAAQAQCNNSADGKLSAVPLQKKSDITFFKTMTDLDVQPVLFIPDVHFGNLQRTGQVFYNTDDDIEGGVLVKRLLRPVDEDYGPPAPKQMKEGSRKVLLYVRKETDEVFDALMLKYPTVKGLLEAISEKYGIPVEKIVKIYKKSKKGILVNMDDNIIEHYSNEDTFILNVESLAEQGYKITLTEI

Target ORF information:

RefSeq Version	XM_018094634.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis grainyhead-like transcription factor 2 (grhl2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094634.1

ORF Insert Sequence:

ATGTTGGGCA CGTCCACTGC ATACAGTAAA CGACTTGTGG TCTTGGTTCC AAATGACGCT 
GTGTTTAACC CTCGCCGTGC CTACACCAGT GAAGATGAGG CCTGGAAATC ATACCTGGAG 
AATCCCTTAA CGGCCGCAAC AAAGGCAATG ATGAGTATAA ATGGGGATGA GGAAAGTGCA 
GCGGCCATTG GACTATTGTA TGATTATTAC AAGGTTCCAA GAGAGAAAAG GTTGTTATCG 
TTAAATAAAA TAAACGAAGG CCATGAAGAT CAGGATAAAA GAAATTGCCT TCCAGCCAAT 
GAAACACCAA GCAACTTGAG CACAGGAGAA AATCGTGTGC AAGTTCTGAA AACGGTTCCT 
GTCAACCTGT CGCTCAACAA TGATACTGTT GAGAGTTCCA ACAGAGAGAA ATACACCACC 
AGCCTGTCTG AAAGCCCCCA GCCGGCTCCT GCGTCGGCGG TCACTGTGGT GAAAGCAGAG 
GAGTTCACAC CAGTGTTTAT GGCCCCTTCA GTGCCTTATA GAAGCGACGG AGAAGAACCC 
CGAGGGGTAA TCTTTGAGCA GACTCACTTT GGAGTACATA GTATTACTAC TCATTCGGAC 
TACCTCAAGG ATGACCAGCG CAGCACTCCG GACAGCACTT ACAATGAAAG TTTTAAAGAA 
ACCACTGAGA AATACCGGAC CCCTTCAGTA GGAACAGAGG AATTTCTTTA TGAACAAACT 
GCAAGCAGCA CCTTTCAGTA TACATTAGAA GCCACAAAAT CTCTGCGCCA GAAGCAAGGA 
GAGGGGCCAA TGACCTATTT AAACAAAGGA CAATTTTATG CCATCACTCT GAGCGAAACT 
GGAGCCAACA AGTGTTTCCG ACATCCCATC AGCAAAGTCA GGATTTTCAT CACCGTCAAT 
TGCCTGAGTA CTGATTTTTC CTCGCAGAAA GGGGTCAAAG GGCTGCCTCT TATGATTCAG 
ATTGACACAT ACAGTTACAA TAACCGCAGC AACAAGCCAA TCCACCGGGC ATACTGCCAG 
ATCAAGGTCT TTTGTGATAA GGGAGCGGAA AGAAAAATCC GTGACGAGGA ACGTAAACAG 
AACAGAAAGA AAGGCAAGAG CCAGGCGGCA CAGGCTCAGT GCAACAATTC AGCCGACGGC 
AAGTTATCAG CAGTGCCCTT GCAGAAGAAA AGTGACATTA CCTTCTTCAA AACAATGACC 
GACTTGGATG TGCAGCCTGT TCTCTTTATT CCTGATGTCC ACTTTGGGAA CCTTCAAAGG 
ACCGGCCAGG TTTTCTACAA CACCGATGAT GACATTGAAG GTGGCGTCTT GGTGAAACGG 
TTGCTTCGCC CTGTGGATGA AGACTATGGC CCTCCTGCAC CCAAGCAAAT GAAAGAAGGA 
AGCAGGAAAG TGCTTCTATA CGTAAGGAAA GAAACTGATG AAGTATTCGA CGCCTTGATG 
CTGAAGTACC CAACTGTTAA AGGGCTCCTC GAGGCTATAT CTGAAAAATA TGGGATCCCC 
GTGGAAAAGA TTGTGAAGAT TTATAAGAAG AGCAAAAAAG GGATCTTGGT CAATATGGAT 
GACAACATCA TTGAACACTA CTCCAATGAG GACACATTCA TTCTAAATGT GGAAAGCTTG 
GCGGAACAAG GCTACAAGAT CACCCTGACA GAAATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grhl2 ( XM_031903346.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759206.1 Xenopus tropicalis grainyhead like transcription factor 2 (grhl2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa59652
Clone ID Related Accession (Same CDS sequence)	XM_031903346.1
Accession Version	XM_031903346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	grainyhead-like protein 2 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACAAG AGACGGACAA TAAACGACTT GTGGTCTTGG TTCCAAATGA CGCTGTGTTT 
AACCCTCGCC GTGCCTACAC CAGTGAAGAT GAGGCCTGGA AATCATACCT GGAGAATCCC 
TTAACGGCCG CAACAAAGGC AATGATGAGT ATAAATGGGG ATGAGGAAAG TGCAGCGGCC 
ATTGGACTAT TGTATGATTA TTACAAGGTT CCAAGAGAGA AAAGGTTGTT ATCGTTAAAT 
AAAATAAACG AAGGCCATGA AGATCAGGAT AAAAGAAATT GCCTTCCAGC CAATGAAACA 
CCAAGCAACT TGAGCACAGG AGAAAATCGT GTGCAAGTTC TGAAAACGGT TCCTGTCAAC 
CTGTCGCTCA ACAATGATAC TGTTGAGAGT TCCAACAGAG AGAAATACAC CACCAGCCTG 
TCTGAAAGCC CCCAGCCGGC TCCTGCGTCG GCGGTCACCG TGGTGAAAGC AGAGGAGTTC 
ACACCAGTGT TTATGGCCCC TTCAGTGCCT TATAGAAGCG ACGGAGAAGA ACCCCGAGGG 
GTAATCTTTG AGCAGACTCA CTTTGGAGTA CATAGTATTA CTACTCATTC GGACTACCTC 
AAGGATGACC AGCGCAGCAC TCCGGACAGC ACTTACAATG AAAGTTTTAA AGAAACCACT 
GAGAAATACC GGACCCCTTC AGTAGGAACA GAGGAATTTC TTTATGAACA AACTGCAAGC 
ACCTTTCAGT ATACATTAGA AGCCACAAAA TCTCTGCGCC AGAAGCAAGG AGAGGGGCCA 
ATGACCTATT TAAACAAAGG ACAATTTTAT GCCATCACTC TGAGCGAAAC TGGAGCCAAC 
AAGTGTTTCC GACATCCCAT CAGCAAAGTC AGGAGTGTAA TTATGGTCGT ATTCAGTGAA 
GATAAAAACC GAGACGAACA GTTAAAATAT TGGAAATACT GGCACTCGCG ACAGCATACA 
GCAAAGCAGC GGGTTCTGGA CATTGCTGAC TACAAAGAAA GCTTTAACAC TATAGGCAAT 
ATTGAAGAAA TTGCTTACAA TGCGGTGTCT TTCACATGGG ATGTCAATGA AGAAGCCAAG 
ATTTTCATCA CCGTCAATTG CCTGAGTACT GATTTTTCCT CGCAGAAAGG GGTCAAAGGG 
CTGCCTCTTA TGATTCAGAT TGACACATAC AGTTACAATA ACCGCAGCAA CAAGCCAATC 
CACCGGGCAT ACTGCCAGAT CAAGGTCTTT TGTGACAAGG GAGCGGAAAG AAAAATCCGT 
GACGAGGAAC GTAAACAGAA CAGAAAGAAA GGCAAGAGCC AGGCGGCACA GGCTCAGTGC 
AACAATTCAG CCGACGGCAA GTTATCAGCA GTGCCCTTGC AGAAGAAAAG TGACATTACC 
TTCTTCAAAA CAATGACCGA CTTGGATGTG CAGCCTGTTC TCTTTATTCC TGATGTCCAC 
TTTGGGAACC TTCAAAGGAC CGGCCAGGTT TTCTACAACA CCGATGATGA CATTGAAGGT 
GGCGTCTTGG TGAAACGGTT GCTTCGCCCT GTGGATGAAG ACTATGGCCC TCCTGCACCC 
AAGCAAATGA AAGAAGGAAG CAGGAAAGTG CTTCTATACG TAAGGAAAGA AACTGATGAA 
GTATTCGACG CCTTGATGCT GAAGTACCCA ACTGTTAAAG GGCTCCTCGA GGCTATATCT 
GAAAAATATG GGATCCCCGT GGAAAAGATT GTGAAGATTT ATAAGAAGAG CAAAAAAGGG 
ATCTTGGTCA ATATGGATGA CAACATCATT GAACACTACT CCAATGAGGA CACATTCATT 
CTAAATGTGG AAAGCTTGGC GGAACAAGGC TACAAGATCA CCCTGACAGA AATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759206.1
CDS	177..2033
Translation	MSQETDNKRLVVLVPNDAVFNPRRAYTSEDEAWKSYLENPLTAATKAMMSINGDEESAAAIGLLYDYYKVPREKRLLSLNKINEGHEDQDKRNCLPANETPSNLSTGENRVQVLKTVPVNLSLNNDTVESSNREKYTTSLSESPQPAPASAVTVVKAEEFTPVFMAPSVPYRSDGEEPRGVIFEQTHFGVHSITTHSDYLKDDQRSTPDSTYNESFKETTEKYRTPSVGTEEFLYEQTASTFQYTLEATKSLRQKQGEGPMTYLNKGQFYAITLSETGANKCFRHPISKVRSVIMVVFSEDKNRDEQLKYWKYWHSRQHTAKQRVLDIADYKESFNTIGNIEEIAYNAVSFTWDVNEEAKIFITVNCLSTDFSSQKGVKGLPLMIQIDTYSYNNRSNKPIHRAYCQIKVFCDKGAERKIRDEERKQNRKKGKSQAAQAQCNNSADGKLSAVPLQKKSDITFFKTMTDLDVQPVLFIPDVHFGNLQRTGQVFYNTDDDIEGGVLVKRLLRPVDEDYGPPAPKQMKEGSRKVLLYVRKETDEVFDALMLKYPTVKGLLEAISEKYGIPVEKIVKIYKKSKKGILVNMDDNIIEHYSNEDTFILNVESLAEQGYKITLTEI

Target ORF information:

RefSeq Version	XM_031903346.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis grainyhead like transcription factor 2 (grhl2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903346.1

ORF Insert Sequence:

ATGTCACAAG AGACGGACAA TAAACGACTT GTGGTCTTGG TTCCAAATGA CGCTGTGTTT 
AACCCTCGCC GTGCCTACAC CAGTGAAGAT GAGGCCTGGA AATCATACCT GGAGAATCCC 
TTAACGGCCG CAACAAAGGC AATGATGAGT ATAAATGGGG ATGAGGAAAG TGCAGCGGCC 
ATTGGACTAT TGTATGATTA TTACAAGGTT CCAAGAGAGA AAAGGTTGTT ATCGTTAAAT 
AAAATAAACG AAGGCCATGA AGATCAGGAT AAAAGAAATT GCCTTCCAGC CAATGAAACA 
CCAAGCAACT TGAGCACAGG AGAAAATCGT GTGCAAGTTC TGAAAACGGT TCCTGTCAAC 
CTGTCGCTCA ACAATGATAC TGTTGAGAGT TCCAACAGAG AGAAATACAC CACCAGCCTG 
TCTGAAAGCC CCCAGCCGGC TCCTGCGTCG GCGGTCACCG TGGTGAAAGC AGAGGAGTTC 
ACACCAGTGT TTATGGCCCC TTCAGTGCCT TATAGAAGCG ACGGAGAAGA ACCCCGAGGG 
GTAATCTTTG AGCAGACTCA CTTTGGAGTA CATAGTATTA CTACTCATTC GGACTACCTC 
AAGGATGACC AGCGCAGCAC TCCGGACAGC ACTTACAATG AAAGTTTTAA AGAAACCACT 
GAGAAATACC GGACCCCTTC AGTAGGAACA GAGGAATTTC TTTATGAACA AACTGCAAGC 
ACCTTTCAGT ATACATTAGA AGCCACAAAA TCTCTGCGCC AGAAGCAAGG AGAGGGGCCA 
ATGACCTATT TAAACAAAGG ACAATTTTAT GCCATCACTC TGAGCGAAAC TGGAGCCAAC 
AAGTGTTTCC GACATCCCAT CAGCAAAGTC AGGAGTGTAA TTATGGTCGT ATTCAGTGAA 
GATAAAAACC GAGACGAACA GTTAAAATAT TGGAAATACT GGCACTCGCG ACAGCATACA 
GCAAAGCAGC GGGTTCTGGA CATTGCTGAC TACAAAGAAA GCTTTAACAC TATAGGCAAT 
ATTGAAGAAA TTGCTTACAA TGCGGTGTCT TTCACATGGG ATGTCAATGA AGAAGCCAAG 
ATTTTCATCA CCGTCAATTG CCTGAGTACT GATTTTTCCT CGCAGAAAGG GGTCAAAGGG 
CTGCCTCTTA TGATTCAGAT TGACACATAC AGTTACAATA ACCGCAGCAA CAAGCCAATC 
CACCGGGCAT ACTGCCAGAT CAAGGTCTTT TGTGACAAGG GAGCGGAAAG AAAAATCCGT 
GACGAGGAAC GTAAACAGAA CAGAAAGAAA GGCAAGAGCC AGGCGGCACA GGCTCAGTGC 
AACAATTCAG CCGACGGCAA GTTATCAGCA GTGCCCTTGC AGAAGAAAAG TGACATTACC 
TTCTTCAAAA CAATGACCGA CTTGGATGTG CAGCCTGTTC TCTTTATTCC TGATGTCCAC 
TTTGGGAACC TTCAAAGGAC CGGCCAGGTT TTCTACAACA CCGATGATGA CATTGAAGGT 
GGCGTCTTGG TGAAACGGTT GCTTCGCCCT GTGGATGAAG ACTATGGCCC TCCTGCACCC 
AAGCAAATGA AAGAAGGAAG CAGGAAAGTG CTTCTATACG TAAGGAAAGA AACTGATGAA 
GTATTCGACG CCTTGATGCT GAAGTACCCA ACTGTTAAAG GGCTCCTCGA GGCTATATCT 
GAAAAATATG GGATCCCCGT GGAAAAGATT GTGAAGATTT ATAAGAAGAG CAAAAAAGGG 
ATCTTGGTCA ATATGGATGA CAACATCATT GAACACTACT CCAATGAGGA CACATTCATT 
CTAAATGTGG AAAGCTTGGC GGAACAAGGC TACAAGATCA CCCTGACAGA AATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grhl2 ( XM_031903347.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759207.1 Xenopus tropicalis grainyhead like transcription factor 2 (grhl2), transcript variant X2, mRNA.

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CloneID	OXa59653
Clone ID Related Accession (Same CDS sequence)	XM_031903347.1
Accession Version	XM_031903347.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	grainyhead-like protein 2 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACAAG AGACGGACAA TAAACGACTT GTGGTCTTGG TTCCAAATGA CGCTGTGTTT 
AACCCTCGCC GTGCCTACAC CAGTGAAGAT GAGGCCTGGA AATCATACCT GGAGAATCCC 
TTAACGGCCG CAACAAAGGC AATGATGAGT ATAAATGGGG ATGAGGAAAG TGCAGCGGCC 
ATTGGACTAT TGTATGATTA TTACAAGGTT CCAAGAGAGA AAAGGTTGTT ATCGTTAAAT 
AAAATAAACG AAGGCCATGA AGATCAGGAT AAAAGAAATT GCCTTCCAGC CAATGAAACA 
CCAAGCAACT TGAGCACAGG AGAAAATCGT GTGCAAGTTC TGAAAACGGT TCCTGTCAAC 
CTGTCGCTCA ACAATGATAC TGTTGAGAGT TCCAACAGAG AGAAATACAC CACCAGCCTG 
TCTGAAAGCC CCCAGCCGGC TCCTGCGTCG GCGGTCACCG TGGTGAAAGC AGAGGAGTTC 
ACACCAGTGT TTATGGCCCC TTCAGTGCCT TATAGAAGCG ACGGAGAAGA ACCCCGAGGG 
GTAATCTTTG AGCAGACTCA CTTTGGAGTA CATAGTATTA CTACTCATTC GGACTACCTC 
AAGGATGACC AGCGCAGCAC TCCGGACAGC ACTTACAATG AAAGTTTTAA AGAAACCACT 
GAGAAATACC GGACCCCTTC AGTAGGAACA GAGGAATTTC TTTATGAACA AACTGCAAGC 
AGCACCTTTC AGTATACATT AGAAGCCACA AAATCTCTGC GCCAGAAGCA AGGAGAGGGG 
CCAATGACCT ATTTAAACAA AGGACAATTT TATGCCATCA CTCTGAGCGA AACTGGAGCC 
AACAAGTGTT TCCGACATCC CATCAGCAAA GTCAGGAGTG TAATTATGGT CGTATTCAGT 
GAAGATAAAA ACCGAGACGA ACAGTTAAAA TATTGGAAAT ACTGGCACTC GCGACAGCAT 
ACAGCAAAGC AGCGGGTTCT GGACATTGCT GACTACAAAG AAAGCTTTAA CACTATAGGC 
AATATTGAAG AAATTGCTTA CAATGCGGTG TCTTTCACAT GGGATGTCAA TGAAGAAGCC 
AAGATTTTCA TCACCGTCAA TTGCCTGAGT ACTGATTTTT CCTCGCAGAA AGGGGTCAAA 
GGGCTGCCTC TTATGATTCA GATTGACACA TACAGTTACA ATAACCGCAG CAACAAGCCA 
ATCCACCGGG CATACTGCCA GATCAAGGTC TTTTGTGACA AGGGAGCGGA AAGAAAAATC 
CGTGACGAGG AACGTAAACA GAACAGAAAG AAAGGCAAGA GCCAGGCGGC ACAGGCTCAG 
TGCAACAATT CCGACGGCAA GTTATCAGCA GTGCCCTTGC AGAAGAAAAG TGACATTACC 
TTCTTCAAAA CAATGACCGA CTTGGATGTG CAGCCTGTTC TCTTTATTCC TGATGTCCAC 
TTTGGGAACC TTCAAAGGAC CGGCCAGGTT TTCTACAACA CCGATGATGA CATTGAAGGT 
GGCGTCTTGG TGAAACGGTT GCTTCGCCCT GTGGATGAAG ACTATGGCCC TCCTGCACCC 
AAGCAAATGA AAGAAGGAAG CAGGAAAGTG CTTCTATACG TAAGGAAAGA AACTGATGAA 
GTATTCGACG CCTTGATGCT GAAGTACCCA ACTGTTAAAG GGCTCCTCGA GGCTATATCT 
GAAAAATATG GGATCCCCGT GGAAAAGATT GTGAAGATTT ATAAGAAGAG CAAAAAAGGG 
ATCTTGGTCA ATATGGATGA CAACATCATT GAACACTACT CCAATGAGGA CACATTCATT 
CTAAATGTGG AAAGCTTGGC GGAACAAGGC TACAAGATCA CCCTGACAGA AATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759207.1
CDS	178..2034
Translation	MSQETDNKRLVVLVPNDAVFNPRRAYTSEDEAWKSYLENPLTAATKAMMSINGDEESAAAIGLLYDYYKVPREKRLLSLNKINEGHEDQDKRNCLPANETPSNLSTGENRVQVLKTVPVNLSLNNDTVESSNREKYTTSLSESPQPAPASAVTVVKAEEFTPVFMAPSVPYRSDGEEPRGVIFEQTHFGVHSITTHSDYLKDDQRSTPDSTYNESFKETTEKYRTPSVGTEEFLYEQTASSTFQYTLEATKSLRQKQGEGPMTYLNKGQFYAITLSETGANKCFRHPISKVRSVIMVVFSEDKNRDEQLKYWKYWHSRQHTAKQRVLDIADYKESFNTIGNIEEIAYNAVSFTWDVNEEAKIFITVNCLSTDFSSQKGVKGLPLMIQIDTYSYNNRSNKPIHRAYCQIKVFCDKGAERKIRDEERKQNRKKGKSQAAQAQCNNSDGKLSAVPLQKKSDITFFKTMTDLDVQPVLFIPDVHFGNLQRTGQVFYNTDDDIEGGVLVKRLLRPVDEDYGPPAPKQMKEGSRKVLLYVRKETDEVFDALMLKYPTVKGLLEAISEKYGIPVEKIVKIYKKSKKGILVNMDDNIIEHYSNEDTFILNVESLAEQGYKITLTEI

Target ORF information:

RefSeq Version	XM_031903347.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis grainyhead like transcription factor 2 (grhl2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903347.1

ORF Insert Sequence:

ATGTCACAAG AGACGGACAA TAAACGACTT GTGGTCTTGG TTCCAAATGA CGCTGTGTTT 
AACCCTCGCC GTGCCTACAC CAGTGAAGAT GAGGCCTGGA AATCATACCT GGAGAATCCC 
TTAACGGCCG CAACAAAGGC AATGATGAGT ATAAATGGGG ATGAGGAAAG TGCAGCGGCC 
ATTGGACTAT TGTATGATTA TTACAAGGTT CCAAGAGAGA AAAGGTTGTT ATCGTTAAAT 
AAAATAAACG AAGGCCATGA AGATCAGGAT AAAAGAAATT GCCTTCCAGC CAATGAAACA 
CCAAGCAACT TGAGCACAGG AGAAAATCGT GTGCAAGTTC TGAAAACGGT TCCTGTCAAC 
CTGTCGCTCA ACAATGATAC TGTTGAGAGT TCCAACAGAG AGAAATACAC CACCAGCCTG 
TCTGAAAGCC CCCAGCCGGC TCCTGCGTCG GCGGTCACCG TGGTGAAAGC AGAGGAGTTC 
ACACCAGTGT TTATGGCCCC TTCAGTGCCT TATAGAAGCG ACGGAGAAGA ACCCCGAGGG 
GTAATCTTTG AGCAGACTCA CTTTGGAGTA CATAGTATTA CTACTCATTC GGACTACCTC 
AAGGATGACC AGCGCAGCAC TCCGGACAGC ACTTACAATG AAAGTTTTAA AGAAACCACT 
GAGAAATACC GGACCCCTTC AGTAGGAACA GAGGAATTTC TTTATGAACA AACTGCAAGC 
AGCACCTTTC AGTATACATT AGAAGCCACA AAATCTCTGC GCCAGAAGCA AGGAGAGGGG 
CCAATGACCT ATTTAAACAA AGGACAATTT TATGCCATCA CTCTGAGCGA AACTGGAGCC 
AACAAGTGTT TCCGACATCC CATCAGCAAA GTCAGGAGTG TAATTATGGT CGTATTCAGT 
GAAGATAAAA ACCGAGACGA ACAGTTAAAA TATTGGAAAT ACTGGCACTC GCGACAGCAT 
ACAGCAAAGC AGCGGGTTCT GGACATTGCT GACTACAAAG AAAGCTTTAA CACTATAGGC 
AATATTGAAG AAATTGCTTA CAATGCGGTG TCTTTCACAT GGGATGTCAA TGAAGAAGCC 
AAGATTTTCA TCACCGTCAA TTGCCTGAGT ACTGATTTTT CCTCGCAGAA AGGGGTCAAA 
GGGCTGCCTC TTATGATTCA GATTGACACA TACAGTTACA ATAACCGCAG CAACAAGCCA 
ATCCACCGGG CATACTGCCA GATCAAGGTC TTTTGTGACA AGGGAGCGGA AAGAAAAATC 
CGTGACGAGG AACGTAAACA GAACAGAAAG AAAGGCAAGA GCCAGGCGGC ACAGGCTCAG 
TGCAACAATT CCGACGGCAA GTTATCAGCA GTGCCCTTGC AGAAGAAAAG TGACATTACC 
TTCTTCAAAA CAATGACCGA CTTGGATGTG CAGCCTGTTC TCTTTATTCC TGATGTCCAC 
TTTGGGAACC TTCAAAGGAC CGGCCAGGTT TTCTACAACA CCGATGATGA CATTGAAGGT 
GGCGTCTTGG TGAAACGGTT GCTTCGCCCT GTGGATGAAG ACTATGGCCC TCCTGCACCC 
AAGCAAATGA AAGAAGGAAG CAGGAAAGTG CTTCTATACG TAAGGAAAGA AACTGATGAA 
GTATTCGACG CCTTGATGCT GAAGTACCCA ACTGTTAAAG GGCTCCTCGA GGCTATATCT 
GAAAAATATG GGATCCCCGT GGAAAAGATT GTGAAGATTT ATAAGAAGAG CAAAAAAGGG 
ATCTTGGTCA ATATGGATGA CAACATCATT GAACACTACT CCAATGAGGA CACATTCATT 
CTAAATGTGG AAAGCTTGGC GGAACAAGGC TACAAGATCA CCCTGACAGA AATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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grhl2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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