mad1l1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	mad1l1
Entrez Gene ID	448774
Full Name	mitotic arrest deficient 1 like 1
Synonyms	hsmad1,tp53i9,txbp181
General protein information	Preferred Names mitotic arrest deficient 1 like 1 Names mitotic spindle assembly checkpoint protein MAD1 MAD1 mitotic arrest deficient-like 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031892606.1	XP_031748466.1	mitotic spindle assembly checkpoint protein MAD1 isoform X2
NM_001006926.1	NP_001006927.1	mitotic spindle assembly checkpoint protein MAD1
XM_031892604.1	XP_031748464.1	mitotic spindle assembly checkpoint protein MAD1 isoform X1
XM_031892605.1	XP_031748465.1	mitotic spindle assembly checkpoint protein MAD1 isoform X1
XM_031892607.1	XP_031748467.1	mitotic spindle assembly checkpoint protein MAD1 isoform X3
XM_031892608.1	XP_031748468.1	mitotic spindle assembly checkpoint protein MAD1 isoform X4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAD1L1	XP_518933.2
Bos taurus (cattle)	MAD1L1	NP_001095514.1
Gallus gallus (chicken)	MAD1L1	XP_004945193.1
	MAD1L1	XP_001102100.1
Canis lupus familiaris (dog)	MAD1L1	XP_852066.2
Mus musculus (house mouse)	Mad1l1	NP_034882.2
Drosophila melanogaster (fruit fly)	Mad1	NP_610477.2
Homo sapiens (human)	MAD1L1	NP_001013858.1
Rattus norvegicus (Norway rat)	Mad1l1	NP_001102857.1
Danio rerio (zebrafish)	mad1l1	NP_957206.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006632	XP_316663.4
Xenopus tropicalis (tropical clawed frog)	mad1l1	NP_001006927.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mad1l1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mad1l1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa23773	NM_001006926.1 Latest version!	Xenopus tropicalis mitotic arrest deficient 1 like 1 (mad1l1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa23773	XM_031892605.1 Latest version!	Xenopus tropicalis mitotic arrest deficient 1 like 1 (mad1l1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa23773	XM_031892604.1 Latest version!	Xenopus tropicalis mitotic arrest deficient 1 like 1 (mad1l1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa65987	XM_031892606.1 Latest version!	Xenopus tropicalis mitotic arrest deficient 1 like 1 (mad1l1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa65988	XM_031892607.1 Latest version!	Xenopus tropicalis mitotic arrest deficient 1 like 1 (mad1l1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa65989	XM_031892608.1 Latest version!	Xenopus tropicalis mitotic arrest deficient 1 like 1 (mad1l1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mad1l1 ( NM_001006926.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001006927.1 Xenopus tropicalis mitotic arrest deficient 1 like 1 (mad1l1), mRNA.

Price & Availability↑

CloneID	OXa23773
Clone ID Related Accession (Same CDS sequence)	NM_001006926.1 , XM_031892604.1 , XM_031892605.1
Accession Version	NM_001006926.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitotic spindle assembly checkpoint protein MAD1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC076947.1. ##Evidence-Data-START## Transcript exon combination :: BC076947.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGATGACA GTGAAGACAA CACCACTGTC ATTTCCACAC TACGGTCATT TAATAAATTT 
CTTTCTCAGC CATTGGAAGG GACAGCACCT TCTCTTGGTA CTTCAACATC TACTGGAGCT 
TCCCTTCAAA TGCAGTTTCA GCAGCGCTTT CTGCTGGAAG ACCAGGCAGC GCAGATTCGT 
TCAAAGTCTC ATCTTATTCA GGTTGAACGT GAAAAGATGC AGATGGAACT AAGCCACAAA 
CGGGCCCGTA TTGAGCTTGA GAAAGCAGCC AGCACCAACG CCAAGAATTA CGAGCGGGAA 
GCAGACAGGA ACCAGGAGCT CATCACCCGC ATCAAGGCTC TAGAGGAAAG GGAGAATGAT 
TTTCAGAACA AACTTCAAGA GCAGAATGAA ATGATCAAAT CCTACAAGAA AACCATAGAG 
GCTCAAAGCA AGAAGCTGCT GGAGAAAGAG GACAAACTGT CTGAATTAAA TGAGAACATC 
AGTGTGTTAA AAGGAAAAGC AACTGAGCTT CAGTGGAAGA TAATGAACCA AGAAATGCAA 
ATAAAGACCC AGGAGACTGA GAAGCAGGAG ATGACAGAGC AGCTGGAAAT CCATCGCAAA 
AAGCTGCAAG AAAGCAATGA GAAGATGCAG GCACTGCATG AGCTTCAGGC GCAAAATGTT 
GATAATGAAC AGAAAATCAA GAGCCTGGAA CAGAAACTGT CAGCCCAGGA ACAGGATGCT 
GCCATTGTGA AGAGCATGAA ATCTGATTTG GCCAAATTGC CGAAACTGGA AAGGGAATTA 
CAGCAGCTCA GAGATGAGAA TGCTTACCAC AGAGAAATGA AAGAAAACAA TGCATTGCTT 
AAAGAAGAAG TAGAGGGTCT GCGCAGAGCC GCAGAACGCT TTAATAAAAT GAAAGAAGAC 
CTGGTGGGAT TGGAAATTGA GAAAGAACAA CTGCTAAAGA AGCTGAAACT TTGGGAGAGC 
CTTGAGCATA CAACTGGATT AAACATAAGA ACTCCGGATG ATCTTTCCAG ACAAATCATT 
GCAGTGCAAC AGAGAGAGCT GAAGCTGAAA GAGGAGAACA TGACAATTCA GAACAGTGCA 
AGAATGCTGG AAACATCACG GCAGCAGCTG CGAGAAGAAC TTTTAAAAGT CCAGGCCGGT 
TTTCTAGAAG AAAAGAAAAG GAGAGAGCAT CAGGAGGCAT TTGTACGCCG TTTGCAAAAG 
CGGGTCCTTC TTCTGACTAA GGAACGGGAT GGAATGAGAG CCATTTTGGA TTCTTATGAT 
AGTGAGTTGA CCCCGACCGA ACATTCTCCT CAACTGAGCC GCCGCCTGCG GGAAGCAGAA 
GATATGGTAC AGAAGGTTCA GGACCACAAT GCTGAAATGG AGACACAGTT GTCAGAGGCG 
CTGGAAGAAA CAGGCATTCA GAAGCAGAAG TCTGATCTTT TAATGGCAGA GCTGAAAGTC 
TTAAAGTCTC AGATGGGCTC ATCAGATCAA AACATCTCTT TTACAAATGA GGCAATGAAC 
ACTCTCAGAT TGAAAATAGA GGAGCTTGAA GCTGAACGTG GTAGATTAGA GGAAGAGAAC 
AAAATCTTAG AGATGAGACT GGAACGCTTG AATCTTCAGG GATGCTACGA CCCAAGCAAA 
ACAAAAGTGA TCCACTTGGG TCTAAATCCC TCAAGTCAAG CCAAGCAGCA ACGTGCGGAA 
ACAGTGCGCC AATTACAGGA AGAATGCAGT AAGCTGCGGG AGCTTGTAAG GATTTTAGAG 
GGAGGAGCCC AAATCCCAGA CAAACTGGAG GCAGCTGGAA GTGTGCAGTC TTCCCAGGAA 
CTTACAGAAC TGAAGAAGCA GTTGGAGAGT GCTGAATTGA AAAACCAGCG TCTTCGGGAG 
GTCTTCCAGA CGAAAATTCA TGAGTTCCGT ACCGCGTGCT ACATGCTGAC AGGGTATCGC 
ATTGATATCA CCACTGAGAA TCAGTACCGC CTTACATCCA TGTATGCGGA ACAGAAAGAA 
GATAACTTGT TGTTCAAGGC GTCCGGCTCC TCCGGAGGGA AGATGCAGCT GCTAGAGACG 
GACTTCTCTT TGACCTTGCG TGATTTTATT GATCTTCACC TACATCACCA GAACAGCATC 
CCAGCATTCC TCAGTGCAGT CACCCTTGAC CTCTTCAGTC GCCAGACCTT TGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001006927.1
CDS	68..2224
Translation	MDDSEDNTTVISTLRSFNKFLSQPLEGTAPSLGTSTSTGASLQMQFQQRFLLEDQAAQIRSKSHLIQVEREKMQMELSHKRARIELEKAASTNAKNYEREADRNQELITRIKALEERENDFQNKLQEQNEMIKSYKKTIEAQSKKLLEKEDKLSELNENISVLKGKATELQWKIMNQEMQIKTQETEKQEMTEQLEIHRKKLQESNEKMQALHELQAQNVDNEQKIKSLEQKLSAQEQDAAIVKSMKSDLAKLPKLERELQQLRDENAYHREMKENNALLKEEVEGLRRAAERFNKMKEDLVGLEIEKEQLLKKLKLWESLEHTTGLNIRTPDDLSRQIIAVQQRELKLKEENMTIQNSARMLETSRQQLREELLKVQAGFLEEKKRREHQEAFVRRLQKRVLLLTKERDGMRAILDSYDSELTPTEHSPQLSRRLREAEDMVQKVQDHNAEMETQLSEALEETGIQKQKSDLLMAELKVLKSQMGSSDQNISFTNEAMNTLRLKIEELEAERGRLEEENKILEMRLERLNLQGCYDPSKTKVIHLGLNPSSQAKQQRAETVRQLQEECSKLRELVRILEGGAQIPDKLEAAGSVQSSQELTELKKQLESAELKNQRLREVFQTKIHEFRTACYMLTGYRIDITTENQYRLTSMYAEQKEDNLLFKASGSSGGKMQLLETDFSLTLRDFIDLHLHHQNSIPAFLSAVTLDLFSRQTFA

Target ORF information:

RefSeq Version	NM_001006926.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitotic arrest deficient 1 like 1 (mad1l1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001006926.1

ORF Insert Sequence:

ATGGATGACA GTGAAGACAA CACCACTGTC ATTTCCACAC TACGGTCATT TAATAAATTT 
CTTTCTCAGC CATTGGAAGG GACAGCACCT TCTCTTGGTA CTTCAACATC TACTGGAGCT 
TCCCTTCAAA TGCAGTTTCA GCAGCGCTTT CTGCTGGAAG ACCAGGCAGC GCAGATTCGT 
TCAAAGTCTC ATCTTATTCA GGTTGAACGT GAAAAGATGC AGATGGAACT AAGCCACAAA 
CGGGCCCGTA TTGAGCTTGA GAAAGCAGCC AGCACCAACG CCAAGAATTA CGAGCGGGAA 
GCAGACAGGA ACCAGGAGCT CATCACCCGC ATCAAGGCTC TAGAGGAAAG GGAGAATGAT 
TTTCAGAACA AACTTCAAGA GCAGAATGAA ATGATCAAAT CCTACAAGAA AACCATAGAG 
GCTCAAAGCA AGAAGCTGCT GGAGAAAGAG GACAAACTGT CTGAATTAAA TGAGAACATC 
AGTGTGTTAA AAGGAAAAGC AACTGAGCTT CAGTGGAAGA TAATGAACCA AGAAATGCAA 
ATAAAGACCC AGGAGACTGA GAAGCAGGAG ATGACAGAGC AGCTGGAAAT CCATCGCAAA 
AAGCTGCAAG AAAGCAATGA GAAGATGCAG GCACTGCATG AGCTTCAGGC GCAAAATGTT 
GATAATGAAC AGAAAATCAA GAGCCTGGAA CAGAAACTGT CAGCCCAGGA ACAGGATGCT 
GCCATTGTGA AGAGCATGAA ATCTGATTTG GCCAAATTGC CGAAACTGGA AAGGGAATTA 
CAGCAGCTCA GAGATGAGAA TGCTTACCAC AGAGAAATGA AAGAAAACAA TGCATTGCTT 
AAAGAAGAAG TAGAGGGTCT GCGCAGAGCC GCAGAACGCT TTAATAAAAT GAAAGAAGAC 
CTGGTGGGAT TGGAAATTGA GAAAGAACAA CTGCTAAAGA AGCTGAAACT TTGGGAGAGC 
CTTGAGCATA CAACTGGATT AAACATAAGA ACTCCGGATG ATCTTTCCAG ACAAATCATT 
GCAGTGCAAC AGAGAGAGCT GAAGCTGAAA GAGGAGAACA TGACAATTCA GAACAGTGCA 
AGAATGCTGG AAACATCACG GCAGCAGCTG CGAGAAGAAC TTTTAAAAGT CCAGGCCGGT 
TTTCTAGAAG AAAAGAAAAG GAGAGAGCAT CAGGAGGCAT TTGTACGCCG TTTGCAAAAG 
CGGGTCCTTC TTCTGACTAA GGAACGGGAT GGAATGAGAG CCATTTTGGA TTCTTATGAT 
AGTGAGTTGA CCCCGACCGA ACATTCTCCT CAACTGAGCC GCCGCCTGCG GGAAGCAGAA 
GATATGGTAC AGAAGGTTCA GGACCACAAT GCTGAAATGG AGACACAGTT GTCAGAGGCG 
CTGGAAGAAA CAGGCATTCA GAAGCAGAAG TCTGATCTTT TAATGGCAGA GCTGAAAGTC 
TTAAAGTCTC AGATGGGCTC ATCAGATCAA AACATCTCTT TTACAAATGA GGCAATGAAC 
ACTCTCAGAT TGAAAATAGA GGAGCTTGAA GCTGAACGTG GTAGATTAGA GGAAGAGAAC 
AAAATCTTAG AGATGAGACT GGAACGCTTG AATCTTCAGG GATGCTACGA CCCAAGCAAA 
ACAAAAGTGA TCCACTTGGG TCTAAATCCC TCAAGTCAAG CCAAGCAGCA ACGTGCGGAA 
ACAGTGCGCC AATTACAGGA AGAATGCAGT AAGCTGCGGG AGCTTGTAAG GATTTTAGAG 
GGAGGAGCCC AAATCCCAGA CAAACTGGAG GCAGCTGGAA GTGTGCAGTC TTCCCAGGAA 
CTTACAGAAC TGAAGAAGCA GTTGGAGAGT GCTGAATTGA AAAACCAGCG TCTTCGGGAG 
GTCTTCCAGA CGAAAATTCA TGAGTTCCGT ACCGCGTGCT ACATGCTGAC AGGGTATCGC 
ATTGATATCA CCACTGAGAA TCAGTACCGC CTTACATCCA TGTATGCGGA ACAGAAAGAA 
GATAACTTGT TGTTCAAGGC GTCCGGCTCC TCCGGAGGGA AGATGCAGCT GCTAGAGACG 
GACTTCTCTT TGACCTTGCG TGATTTTATT GATCTTCACC TACATCACCA GAACAGCATC 
CCAGCATTCC TCAGTGCAGT CACCCTTGAC CTCTTCAGTC GCCAGACCTT TGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mad1l1 ( XM_031892604.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748464.1 Xenopus tropicalis mitotic arrest deficient 1 like 1 (mad1l1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa23773
Clone ID Related Accession (Same CDS sequence)	NM_001006926.1 , XM_031892604.1 , XM_031892605.1
Accession Version	XM_031892604.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitotic spindle assembly checkpoint protein MAD1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACA GTGAAGACAA CACCACTGTC ATTTCCACAC TACGGTCATT TAATAAATTT 
CTTTCTCAGC CATTGGAAGG GACAGCACCT TCTCTTGGTA CTTCAACATC TACTGGAGCT 
TCCCTTCAAA TGCAGTTTCA GCAGCGCTTT CTGCTGGAAG ACCAGGCAGC GCAGATTCGT 
TCAAAGTCTC ATCTTATTCA GGTTGAACGT GAAAAGATGC AGATGGAACT AAGCCACAAA 
CGGGCCCGTA TTGAGCTTGA GAAAGCAGCC AGCACCAACG CCAAGAATTA CGAGCGGGAA 
GCAGACAGGA ACCAGGAGCT CATCACCCGC ATCAAGGCTC TAGAGGAAAG GGAGAATGAT 
TTTCAGAACA AACTTCAAGA GCAGAATGAA ATGATCAAAT CCTACAAGAA AACCATAGAG 
GCTCAAAGCA AGAAGCTGCT GGAGAAAGAG GACAAACTGT CTGAATTAAA TGAGAACATC 
AGTGTGTTAA AAGGAAAAGC AACTGAGCTT CAGTGGAAGA TAATGAACCA AGAAATGCAA 
ATAAAGACCC AGGAGACTGA GAAGCAGGAG ATGACAGAGC AGCTGGAAAT CCATCGCAAA 
AAGCTGCAAG AAAGCAATGA GAAGATGCAG GCACTGCATG AGCTTCAGGC GCAAAATGTT 
GATAATGAAC AGAAAATCAA GAGCCTGGAA CAGAAACTGT CAGCCCAGGA ACAGGATGCT 
GCCATTGTGA AGAGCATGAA ATCTGATTTG GCCAAATTGC CGAAACTGGA AAGGGAATTA 
CAGCAGCTCA GAGATGAGAA TGCTTACCAC AGAGAAATGA AAGAAAACAA TGCATTGCTT 
AAAGAAGAAG TAGAGGGTCT GCGCAGAGCC GCAGAACGCT TTAATAAAAT GAAAGAAGAC 
CTGGTGGGAT TGGAAATTGA GAAAGAACAA CTGCTAAAGA AGCTGAAACT TTGGGAGAGC 
CTTGAGCATA CAACTGGATT AAACATAAGA ACTCCGGATG ATCTTTCCAG ACAAATCATT 
GCAGTGCAAC AGAGAGAGCT GAAGCTGAAA GAGGAGAACA TGACAATTCA GAACAGTGCA 
AGAATGCTGG AAACATCACG GCAGCAGCTG CGAGAAGAAC TTTTAAAAGT CCAGGCCGGT 
TTTCTAGAAG AAAAGAAAAG GAGAGAGCAT CAGGAGGCAT TTGTACGCCG TTTGCAAAAG 
CGGGTCCTTC TTCTGACTAA GGAACGGGAT GGAATGAGAG CCATTTTGGA TTCTTATGAT 
AGTGAGTTGA CCCCGACCGA ACATTCTCCT CAACTGAGCC GCCGCCTGCG GGAAGCAGAA 
GATATGGTAC AGAAGGTTCA GGACCACAAT GCTGAAATGG AGACACAGTT GTCAGAGGCG 
CTGGAAGAAA CAGGCATTCA GAAGCAGAAG TCTGATCTTT TAATGGCAGA GCTGAAAGTC 
TTAAAGTCTC AGATGGGCTC ATCAGATCAA AACATCTCTT TTACAAATGA GGCAATGAAC 
ACTCTCAGAT TGAAAATAGA GGAGCTTGAA GCTGAACGTG GTAGATTAGA GGAAGAGAAC 
AAAATCTTAG AGATGAGACT GGAACGCTTG AATCTTCAGG GATGCTACGA CCCAAGCAAA 
ACAAAAGTGA TCCACTTGGG TCTAAATCCC TCAAGTCAAG CCAAGCAGCA ACGTGCGGAA 
ACAGTGCGCC AATTACAGGA AGAATGCAGT AAGCTGCGGG AGCTTGTAAG GATTTTAGAG 
GGAGGAGCCC AAATCCCAGA CAAACTGGAG GCAGCTGGAA GTGTGCAGTC TTCCCAGGAA 
CTTACAGAAC TGAAGAAGCA GTTGGAGAGT GCTGAATTGA AAAACCAGCG TCTTCGGGAG 
GTCTTCCAGA CGAAAATTCA TGAGTTCCGT ACCGCGTGCT ACATGCTGAC AGGGTATCGC 
ATTGATATCA CCACTGAGAA TCAGTACCGC CTTACATCCA TGTATGCGGA ACAGAAAGAA 
GATAACTTGT TGTTCAAGGC GTCCGGCTCC TCCGGAGGGA AGATGCAGCT GCTAGAGACG 
GACTTCTCTT TGACCTTGCG TGATTTTATT GATCTTCACC TACATCACCA GAACAGCATC 
CCAGCATTCC TCAGTGCAGT CACCCTTGAC CTCTTCAGTC GCCAGACCTT TGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748464.1
CDS	125..2281
Translation	MDDSEDNTTVISTLRSFNKFLSQPLEGTAPSLGTSTSTGASLQMQFQQRFLLEDQAAQIRSKSHLIQVEREKMQMELSHKRARIELEKAASTNAKNYEREADRNQELITRIKALEERENDFQNKLQEQNEMIKSYKKTIEAQSKKLLEKEDKLSELNENISVLKGKATELQWKIMNQEMQIKTQETEKQEMTEQLEIHRKKLQESNEKMQALHELQAQNVDNEQKIKSLEQKLSAQEQDAAIVKSMKSDLAKLPKLERELQQLRDENAYHREMKENNALLKEEVEGLRRAAERFNKMKEDLVGLEIEKEQLLKKLKLWESLEHTTGLNIRTPDDLSRQIIAVQQRELKLKEENMTIQNSARMLETSRQQLREELLKVQAGFLEEKKRREHQEAFVRRLQKRVLLLTKERDGMRAILDSYDSELTPTEHSPQLSRRLREAEDMVQKVQDHNAEMETQLSEALEETGIQKQKSDLLMAELKVLKSQMGSSDQNISFTNEAMNTLRLKIEELEAERGRLEEENKILEMRLERLNLQGCYDPSKTKVIHLGLNPSSQAKQQRAETVRQLQEECSKLRELVRILEGGAQIPDKLEAAGSVQSSQELTELKKQLESAELKNQRLREVFQTKIHEFRTACYMLTGYRIDITTENQYRLTSMYAEQKEDNLLFKASGSSGGKMQLLETDFSLTLRDFIDLHLHHQNSIPAFLSAVTLDLFSRQTFA

Target ORF information:

RefSeq Version	XM_031892604.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitotic arrest deficient 1 like 1 (mad1l1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892604.1

ORF Insert Sequence:

ATGGATGACA GTGAAGACAA CACCACTGTC ATTTCCACAC TACGGTCATT TAATAAATTT 
CTTTCTCAGC CATTGGAAGG GACAGCACCT TCTCTTGGTA CTTCAACATC TACTGGAGCT 
TCCCTTCAAA TGCAGTTTCA GCAGCGCTTT CTGCTGGAAG ACCAGGCAGC GCAGATTCGT 
TCAAAGTCTC ATCTTATTCA GGTTGAACGT GAAAAGATGC AGATGGAACT AAGCCACAAA 
CGGGCCCGTA TTGAGCTTGA GAAAGCAGCC AGCACCAACG CCAAGAATTA CGAGCGGGAA 
GCAGACAGGA ACCAGGAGCT CATCACCCGC ATCAAGGCTC TAGAGGAAAG GGAGAATGAT 
TTTCAGAACA AACTTCAAGA GCAGAATGAA ATGATCAAAT CCTACAAGAA AACCATAGAG 
GCTCAAAGCA AGAAGCTGCT GGAGAAAGAG GACAAACTGT CTGAATTAAA TGAGAACATC 
AGTGTGTTAA AAGGAAAAGC AACTGAGCTT CAGTGGAAGA TAATGAACCA AGAAATGCAA 
ATAAAGACCC AGGAGACTGA GAAGCAGGAG ATGACAGAGC AGCTGGAAAT CCATCGCAAA 
AAGCTGCAAG AAAGCAATGA GAAGATGCAG GCACTGCATG AGCTTCAGGC GCAAAATGTT 
GATAATGAAC AGAAAATCAA GAGCCTGGAA CAGAAACTGT CAGCCCAGGA ACAGGATGCT 
GCCATTGTGA AGAGCATGAA ATCTGATTTG GCCAAATTGC CGAAACTGGA AAGGGAATTA 
CAGCAGCTCA GAGATGAGAA TGCTTACCAC AGAGAAATGA AAGAAAACAA TGCATTGCTT 
AAAGAAGAAG TAGAGGGTCT GCGCAGAGCC GCAGAACGCT TTAATAAAAT GAAAGAAGAC 
CTGGTGGGAT TGGAAATTGA GAAAGAACAA CTGCTAAAGA AGCTGAAACT TTGGGAGAGC 
CTTGAGCATA CAACTGGATT AAACATAAGA ACTCCGGATG ATCTTTCCAG ACAAATCATT 
GCAGTGCAAC AGAGAGAGCT GAAGCTGAAA GAGGAGAACA TGACAATTCA GAACAGTGCA 
AGAATGCTGG AAACATCACG GCAGCAGCTG CGAGAAGAAC TTTTAAAAGT CCAGGCCGGT 
TTTCTAGAAG AAAAGAAAAG GAGAGAGCAT CAGGAGGCAT TTGTACGCCG TTTGCAAAAG 
CGGGTCCTTC TTCTGACTAA GGAACGGGAT GGAATGAGAG CCATTTTGGA TTCTTATGAT 
AGTGAGTTGA CCCCGACCGA ACATTCTCCT CAACTGAGCC GCCGCCTGCG GGAAGCAGAA 
GATATGGTAC AGAAGGTTCA GGACCACAAT GCTGAAATGG AGACACAGTT GTCAGAGGCG 
CTGGAAGAAA CAGGCATTCA GAAGCAGAAG TCTGATCTTT TAATGGCAGA GCTGAAAGTC 
TTAAAGTCTC AGATGGGCTC ATCAGATCAA AACATCTCTT TTACAAATGA GGCAATGAAC 
ACTCTCAGAT TGAAAATAGA GGAGCTTGAA GCTGAACGTG GTAGATTAGA GGAAGAGAAC 
AAAATCTTAG AGATGAGACT GGAACGCTTG AATCTTCAGG GATGCTACGA CCCAAGCAAA 
ACAAAAGTGA TCCACTTGGG TCTAAATCCC TCAAGTCAAG CCAAGCAGCA ACGTGCGGAA 
ACAGTGCGCC AATTACAGGA AGAATGCAGT AAGCTGCGGG AGCTTGTAAG GATTTTAGAG 
GGAGGAGCCC AAATCCCAGA CAAACTGGAG GCAGCTGGAA GTGTGCAGTC TTCCCAGGAA 
CTTACAGAAC TGAAGAAGCA GTTGGAGAGT GCTGAATTGA AAAACCAGCG TCTTCGGGAG 
GTCTTCCAGA CGAAAATTCA TGAGTTCCGT ACCGCGTGCT ACATGCTGAC AGGGTATCGC 
ATTGATATCA CCACTGAGAA TCAGTACCGC CTTACATCCA TGTATGCGGA ACAGAAAGAA 
GATAACTTGT TGTTCAAGGC GTCCGGCTCC TCCGGAGGGA AGATGCAGCT GCTAGAGACG 
GACTTCTCTT TGACCTTGCG TGATTTTATT GATCTTCACC TACATCACCA GAACAGCATC 
CCAGCATTCC TCAGTGCAGT CACCCTTGAC CTCTTCAGTC GCCAGACCTT TGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mad1l1 ( XM_031892605.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748465.1 Xenopus tropicalis mitotic arrest deficient 1 like 1 (mad1l1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa23773
Clone ID Related Accession (Same CDS sequence)	NM_001006926.1 , XM_031892604.1 , XM_031892605.1
Accession Version	XM_031892605.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitotic spindle assembly checkpoint protein MAD1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACA GTGAAGACAA CACCACTGTC ATTTCCACAC TACGGTCATT TAATAAATTT 
CTTTCTCAGC CATTGGAAGG GACAGCACCT TCTCTTGGTA CTTCAACATC TACTGGAGCT 
TCCCTTCAAA TGCAGTTTCA GCAGCGCTTT CTGCTGGAAG ACCAGGCAGC GCAGATTCGT 
TCAAAGTCTC ATCTTATTCA GGTTGAACGT GAAAAGATGC AGATGGAACT AAGCCACAAA 
CGGGCCCGTA TTGAGCTTGA GAAAGCAGCC AGCACCAACG CCAAGAATTA CGAGCGGGAA 
GCAGACAGGA ACCAGGAGCT CATCACCCGC ATCAAGGCTC TAGAGGAAAG GGAGAATGAT 
TTTCAGAACA AACTTCAAGA GCAGAATGAA ATGATCAAAT CCTACAAGAA AACCATAGAG 
GCTCAAAGCA AGAAGCTGCT GGAGAAAGAG GACAAACTGT CTGAATTAAA TGAGAACATC 
AGTGTGTTAA AAGGAAAAGC AACTGAGCTT CAGTGGAAGA TAATGAACCA AGAAATGCAA 
ATAAAGACCC AGGAGACTGA GAAGCAGGAG ATGACAGAGC AGCTGGAAAT CCATCGCAAA 
AAGCTGCAAG AAAGCAATGA GAAGATGCAG GCACTGCATG AGCTTCAGGC GCAAAATGTT 
GATAATGAAC AGAAAATCAA GAGCCTGGAA CAGAAACTGT CAGCCCAGGA ACAGGATGCT 
GCCATTGTGA AGAGCATGAA ATCTGATTTG GCCAAATTGC CGAAACTGGA AAGGGAATTA 
CAGCAGCTCA GAGATGAGAA TGCTTACCAC AGAGAAATGA AAGAAAACAA TGCATTGCTT 
AAAGAAGAAG TAGAGGGTCT GCGCAGAGCC GCAGAACGCT TTAATAAAAT GAAAGAAGAC 
CTGGTGGGAT TGGAAATTGA GAAAGAACAA CTGCTAAAGA AGCTGAAACT TTGGGAGAGC 
CTTGAGCATA CAACTGGATT AAACATAAGA ACTCCGGATG ATCTTTCCAG ACAAATCATT 
GCAGTGCAAC AGAGAGAGCT GAAGCTGAAA GAGGAGAACA TGACAATTCA GAACAGTGCA 
AGAATGCTGG AAACATCACG GCAGCAGCTG CGAGAAGAAC TTTTAAAAGT CCAGGCCGGT 
TTTCTAGAAG AAAAGAAAAG GAGAGAGCAT CAGGAGGCAT TTGTACGCCG TTTGCAAAAG 
CGGGTCCTTC TTCTGACTAA GGAACGGGAT GGAATGAGAG CCATTTTGGA TTCTTATGAT 
AGTGAGTTGA CCCCGACCGA ACATTCTCCT CAACTGAGCC GCCGCCTGCG GGAAGCAGAA 
GATATGGTAC AGAAGGTTCA GGACCACAAT GCTGAAATGG AGACACAGTT GTCAGAGGCG 
CTGGAAGAAA CAGGCATTCA GAAGCAGAAG TCTGATCTTT TAATGGCAGA GCTGAAAGTC 
TTAAAGTCTC AGATGGGCTC ATCAGATCAA AACATCTCTT TTACAAATGA GGCAATGAAC 
ACTCTCAGAT TGAAAATAGA GGAGCTTGAA GCTGAACGTG GTAGATTAGA GGAAGAGAAC 
AAAATCTTAG AGATGAGACT GGAACGCTTG AATCTTCAGG GATGCTACGA CCCAAGCAAA 
ACAAAAGTGA TCCACTTGGG TCTAAATCCC TCAAGTCAAG CCAAGCAGCA ACGTGCGGAA 
ACAGTGCGCC AATTACAGGA AGAATGCAGT AAGCTGCGGG AGCTTGTAAG GATTTTAGAG 
GGAGGAGCCC AAATCCCAGA CAAACTGGAG GCAGCTGGAA GTGTGCAGTC TTCCCAGGAA 
CTTACAGAAC TGAAGAAGCA GTTGGAGAGT GCTGAATTGA AAAACCAGCG TCTTCGGGAG 
GTCTTCCAGA CGAAAATTCA TGAGTTCCGT ACCGCGTGCT ACATGCTGAC AGGGTATCGC 
ATTGATATCA CCACTGAGAA TCAGTACCGC CTTACATCCA TGTATGCGGA ACAGAAAGAA 
GATAACTTGT TGTTCAAGGC GTCCGGCTCC TCCGGAGGGA AGATGCAGCT GCTAGAGACG 
GACTTCTCTT TGACCTTGCG TGATTTTATT GATCTTCACC TACATCACCA GAACAGCATC 
CCAGCATTCC TCAGTGCAGT CACCCTTGAC CTCTTCAGTC GCCAGACCTT TGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748465.1
CDS	52..2208
Translation	MDDSEDNTTVISTLRSFNKFLSQPLEGTAPSLGTSTSTGASLQMQFQQRFLLEDQAAQIRSKSHLIQVEREKMQMELSHKRARIELEKAASTNAKNYEREADRNQELITRIKALEERENDFQNKLQEQNEMIKSYKKTIEAQSKKLLEKEDKLSELNENISVLKGKATELQWKIMNQEMQIKTQETEKQEMTEQLEIHRKKLQESNEKMQALHELQAQNVDNEQKIKSLEQKLSAQEQDAAIVKSMKSDLAKLPKLERELQQLRDENAYHREMKENNALLKEEVEGLRRAAERFNKMKEDLVGLEIEKEQLLKKLKLWESLEHTTGLNIRTPDDLSRQIIAVQQRELKLKEENMTIQNSARMLETSRQQLREELLKVQAGFLEEKKRREHQEAFVRRLQKRVLLLTKERDGMRAILDSYDSELTPTEHSPQLSRRLREAEDMVQKVQDHNAEMETQLSEALEETGIQKQKSDLLMAELKVLKSQMGSSDQNISFTNEAMNTLRLKIEELEAERGRLEEENKILEMRLERLNLQGCYDPSKTKVIHLGLNPSSQAKQQRAETVRQLQEECSKLRELVRILEGGAQIPDKLEAAGSVQSSQELTELKKQLESAELKNQRLREVFQTKIHEFRTACYMLTGYRIDITTENQYRLTSMYAEQKEDNLLFKASGSSGGKMQLLETDFSLTLRDFIDLHLHHQNSIPAFLSAVTLDLFSRQTFA

Target ORF information:

RefSeq Version	XM_031892605.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitotic arrest deficient 1 like 1 (mad1l1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892605.1

ORF Insert Sequence:

ATGGATGACA GTGAAGACAA CACCACTGTC ATTTCCACAC TACGGTCATT TAATAAATTT 
CTTTCTCAGC CATTGGAAGG GACAGCACCT TCTCTTGGTA CTTCAACATC TACTGGAGCT 
TCCCTTCAAA TGCAGTTTCA GCAGCGCTTT CTGCTGGAAG ACCAGGCAGC GCAGATTCGT 
TCAAAGTCTC ATCTTATTCA GGTTGAACGT GAAAAGATGC AGATGGAACT AAGCCACAAA 
CGGGCCCGTA TTGAGCTTGA GAAAGCAGCC AGCACCAACG CCAAGAATTA CGAGCGGGAA 
GCAGACAGGA ACCAGGAGCT CATCACCCGC ATCAAGGCTC TAGAGGAAAG GGAGAATGAT 
TTTCAGAACA AACTTCAAGA GCAGAATGAA ATGATCAAAT CCTACAAGAA AACCATAGAG 
GCTCAAAGCA AGAAGCTGCT GGAGAAAGAG GACAAACTGT CTGAATTAAA TGAGAACATC 
AGTGTGTTAA AAGGAAAAGC AACTGAGCTT CAGTGGAAGA TAATGAACCA AGAAATGCAA 
ATAAAGACCC AGGAGACTGA GAAGCAGGAG ATGACAGAGC AGCTGGAAAT CCATCGCAAA 
AAGCTGCAAG AAAGCAATGA GAAGATGCAG GCACTGCATG AGCTTCAGGC GCAAAATGTT 
GATAATGAAC AGAAAATCAA GAGCCTGGAA CAGAAACTGT CAGCCCAGGA ACAGGATGCT 
GCCATTGTGA AGAGCATGAA ATCTGATTTG GCCAAATTGC CGAAACTGGA AAGGGAATTA 
CAGCAGCTCA GAGATGAGAA TGCTTACCAC AGAGAAATGA AAGAAAACAA TGCATTGCTT 
AAAGAAGAAG TAGAGGGTCT GCGCAGAGCC GCAGAACGCT TTAATAAAAT GAAAGAAGAC 
CTGGTGGGAT TGGAAATTGA GAAAGAACAA CTGCTAAAGA AGCTGAAACT TTGGGAGAGC 
CTTGAGCATA CAACTGGATT AAACATAAGA ACTCCGGATG ATCTTTCCAG ACAAATCATT 
GCAGTGCAAC AGAGAGAGCT GAAGCTGAAA GAGGAGAACA TGACAATTCA GAACAGTGCA 
AGAATGCTGG AAACATCACG GCAGCAGCTG CGAGAAGAAC TTTTAAAAGT CCAGGCCGGT 
TTTCTAGAAG AAAAGAAAAG GAGAGAGCAT CAGGAGGCAT TTGTACGCCG TTTGCAAAAG 
CGGGTCCTTC TTCTGACTAA GGAACGGGAT GGAATGAGAG CCATTTTGGA TTCTTATGAT 
AGTGAGTTGA CCCCGACCGA ACATTCTCCT CAACTGAGCC GCCGCCTGCG GGAAGCAGAA 
GATATGGTAC AGAAGGTTCA GGACCACAAT GCTGAAATGG AGACACAGTT GTCAGAGGCG 
CTGGAAGAAA CAGGCATTCA GAAGCAGAAG TCTGATCTTT TAATGGCAGA GCTGAAAGTC 
TTAAAGTCTC AGATGGGCTC ATCAGATCAA AACATCTCTT TTACAAATGA GGCAATGAAC 
ACTCTCAGAT TGAAAATAGA GGAGCTTGAA GCTGAACGTG GTAGATTAGA GGAAGAGAAC 
AAAATCTTAG AGATGAGACT GGAACGCTTG AATCTTCAGG GATGCTACGA CCCAAGCAAA 
ACAAAAGTGA TCCACTTGGG TCTAAATCCC TCAAGTCAAG CCAAGCAGCA ACGTGCGGAA 
ACAGTGCGCC AATTACAGGA AGAATGCAGT AAGCTGCGGG AGCTTGTAAG GATTTTAGAG 
GGAGGAGCCC AAATCCCAGA CAAACTGGAG GCAGCTGGAA GTGTGCAGTC TTCCCAGGAA 
CTTACAGAAC TGAAGAAGCA GTTGGAGAGT GCTGAATTGA AAAACCAGCG TCTTCGGGAG 
GTCTTCCAGA CGAAAATTCA TGAGTTCCGT ACCGCGTGCT ACATGCTGAC AGGGTATCGC 
ATTGATATCA CCACTGAGAA TCAGTACCGC CTTACATCCA TGTATGCGGA ACAGAAAGAA 
GATAACTTGT TGTTCAAGGC GTCCGGCTCC TCCGGAGGGA AGATGCAGCT GCTAGAGACG 
GACTTCTCTT TGACCTTGCG TGATTTTATT GATCTTCACC TACATCACCA GAACAGCATC 
CCAGCATTCC TCAGTGCAGT CACCCTTGAC CTCTTCAGTC GCCAGACCTT TGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mad1l1 ( XM_031892606.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748466.1 Xenopus tropicalis mitotic arrest deficient 1 like 1 (mad1l1), transcript variant X3, mRNA.

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CloneID	OXa65987
Clone ID Related Accession (Same CDS sequence)	XM_031892606.1
Accession Version	XM_031892606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitotic spindle assembly checkpoint protein MAD1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACA GTGAAGACAA CACCACTGTC ATTTCCACAC TACGGTCATT TAATAAATTT 
CTTTCTCAGC CATTGGAAGG GACAGCACCT TCTCTTGGTA CTTCAACATC TACTGGAGCT 
TCCCTTCAAA TGCAGTTTCA GCAGCGCTTT CTGCTGGAAG ACCAGGCAGC GCAGATTCGT 
TCAAAGTCTC ATCTTATTCA GGTTGAACGT GAAAAGATGC AGATGGAACT AAGCCACAAA 
CGGGCCCGTA TTGAGCTTGA GAAAGCAGCC AGCACCAACG CCAAGAATTA CGAGCGGGAA 
GCAGACAGGA ACCAGGAGCT CATCACCCGC ATCAAGGCTC TAGAGGAAAG GGAGAATGAT 
TTTCAGAACA AACTTCAAGA GCAGAATGAA ATGATCAAAT CCTACAAGAA AACCATAGAG 
GCTCAAAGCA AGAAGCTGCT GGAGAAAGAG GACAAACTGT CTGAATTAAA TGAGAACATC 
AGTGTGTTAA AAGGAAAAGC AACTGAGCTT CAGTGGAAGA TAATGAACCA AGAAATGCAA 
ATAAAGACCC AGGAGACTGA GAAGCAGGAG ATGACAGAGC AGCTGGAAAT CCATCGCAAA 
AAGCTGCAAG AAAGCAATGA GAAGATGCAG GCACTGCATG AGCTTCAGGC GCAAAATGTT 
GATAATGAAC AGAAAATCAA GAGCCTGGAA CAGAAACTGT CAGCCCAGGA ACAGGATGCT 
GCCATTGTGA AGAGCATGAA ATCTGATTTG GCCAAATTGC CGAAACTGGA AAGGGAATTA 
CAGCAGCTCA GAGATGAGAA TGCTTACCAC AGAGAAATGA AAGAAAACAA TGCATTGCTT 
AAAGAAGAAG TAGAGGGTCT GCGCAGAGCC GCAGAACGCT TTAATAAAAT GAAAGAAGAC 
CTGGTGGGAT TGGAAATTGA GAAAGAACAA CTGCTAAAGA AGCTGAAACT TTGGGAGAGC 
CTTGAGCATA CAACTGGATT AAACATAAGT GCAAGAATGC TGGAAACATC ACGGCAGCAG 
CTGCGAGAAG AACTTTTAAA AGTCCAGGCC GGTTTTCTAG AAGAAAAGAA AAGGAGAGAG 
CATCAGGAGG CATTTGTACG CCGTTTGCAA AAGCGGGTCC TTCTTCTGAC TAAGGAACGG 
GATGGAATGA GAGCCATTTT GGATTCTTAT GATAGTGAGT TGACCCCGAC CGAACATTCT 
CCTCAACTGA GCCGCCGCCT GCGGGAAGCA GAAGATATGG TACAGAAGGT TCAGGACCAC 
AATGCTGAAA TGGAGACACA GTTGTCAGAG GCGCTGGAAG AAACAGGCAT TCAGAAGCAG 
AAGTCTGATC TTTTAATGGC AGAGCTGAAA GTCTTAAAGT CTCAGATGGG CTCATCAGAT 
CAAAACATCT CTTTTACAAA TGAGGCAATG AACACTCTCA GATTGAAAAT AGAGGAGCTT 
GAAGCTGAAC GTGGTAGATT AGAGGAAGAG AACAAAATCT TAGAGATGAG ACTGGAACGC 
TTGAATCTTC AGGGATGCTA CGACCCAAGC AAAACAAAAG TGATCCACTT GGGTCTAAAT 
CCCTCAAGTC AAGCCAAGCA GCAACGTGCG GAAACAGTGC GCCAATTACA GGAAGAATGC 
AGTAAGCTGC GGGAGCTTGT AAGGATTTTA GAGGGAGGAG CCCAAATCCC AGACAAACTG 
GAGGCAGCTG GAAGTGTGCA GTCTTCCCAG GAACTTACAG AACTGAAGAA GCAGTTGGAG 
AGTGCTGAAT TGAAAAACCA GCGTCTTCGG GAGGTCTTCC AGACGAAAAT TCATGAGTTC 
CGTACCGCGT GCTACATGCT GACAGGGTAT CGCATTGATA TCACCACTGA GAATCAGTAC 
CGCCTTACAT CCATGTATGC GGAACAGAAA GAAGATAACT TGTTGTTCAA GGCGTCCGGC 
TCCTCCGGAG GGAAGATGCA GCTGCTAGAG ACGGACTTCT CTTTGACCTT GCGTGATTTT 
ATTGATCTTC ACCTACATCA CCAGAACAGC ATCCCAGCAT TCCTCAGTGC AGTCACCCTT 
GACCTCTTCA GTCGCCAGAC CTTTGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748466.1
CDS	210..2279
Translation	MDDSEDNTTVISTLRSFNKFLSQPLEGTAPSLGTSTSTGASLQMQFQQRFLLEDQAAQIRSKSHLIQVEREKMQMELSHKRARIELEKAASTNAKNYEREADRNQELITRIKALEERENDFQNKLQEQNEMIKSYKKTIEAQSKKLLEKEDKLSELNENISVLKGKATELQWKIMNQEMQIKTQETEKQEMTEQLEIHRKKLQESNEKMQALHELQAQNVDNEQKIKSLEQKLSAQEQDAAIVKSMKSDLAKLPKLERELQQLRDENAYHREMKENNALLKEEVEGLRRAAERFNKMKEDLVGLEIEKEQLLKKLKLWESLEHTTGLNISARMLETSRQQLREELLKVQAGFLEEKKRREHQEAFVRRLQKRVLLLTKERDGMRAILDSYDSELTPTEHSPQLSRRLREAEDMVQKVQDHNAEMETQLSEALEETGIQKQKSDLLMAELKVLKSQMGSSDQNISFTNEAMNTLRLKIEELEAERGRLEEENKILEMRLERLNLQGCYDPSKTKVIHLGLNPSSQAKQQRAETVRQLQEECSKLRELVRILEGGAQIPDKLEAAGSVQSSQELTELKKQLESAELKNQRLREVFQTKIHEFRTACYMLTGYRIDITTENQYRLTSMYAEQKEDNLLFKASGSSGGKMQLLETDFSLTLRDFIDLHLHHQNSIPAFLSAVTLDLFSRQTFA

Target ORF information:

RefSeq Version	XM_031892606.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitotic arrest deficient 1 like 1 (mad1l1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892606.1

ORF Insert Sequence:

ATGGATGACA GTGAAGACAA CACCACTGTC ATTTCCACAC TACGGTCATT TAATAAATTT 
CTTTCTCAGC CATTGGAAGG GACAGCACCT TCTCTTGGTA CTTCAACATC TACTGGAGCT 
TCCCTTCAAA TGCAGTTTCA GCAGCGCTTT CTGCTGGAAG ACCAGGCAGC GCAGATTCGT 
TCAAAGTCTC ATCTTATTCA GGTTGAACGT GAAAAGATGC AGATGGAACT AAGCCACAAA 
CGGGCCCGTA TTGAGCTTGA GAAAGCAGCC AGCACCAACG CCAAGAATTA CGAGCGGGAA 
GCAGACAGGA ACCAGGAGCT CATCACCCGC ATCAAGGCTC TAGAGGAAAG GGAGAATGAT 
TTTCAGAACA AACTTCAAGA GCAGAATGAA ATGATCAAAT CCTACAAGAA AACCATAGAG 
GCTCAAAGCA AGAAGCTGCT GGAGAAAGAG GACAAACTGT CTGAATTAAA TGAGAACATC 
AGTGTGTTAA AAGGAAAAGC AACTGAGCTT CAGTGGAAGA TAATGAACCA AGAAATGCAA 
ATAAAGACCC AGGAGACTGA GAAGCAGGAG ATGACAGAGC AGCTGGAAAT CCATCGCAAA 
AAGCTGCAAG AAAGCAATGA GAAGATGCAG GCACTGCATG AGCTTCAGGC GCAAAATGTT 
GATAATGAAC AGAAAATCAA GAGCCTGGAA CAGAAACTGT CAGCCCAGGA ACAGGATGCT 
GCCATTGTGA AGAGCATGAA ATCTGATTTG GCCAAATTGC CGAAACTGGA AAGGGAATTA 
CAGCAGCTCA GAGATGAGAA TGCTTACCAC AGAGAAATGA AAGAAAACAA TGCATTGCTT 
AAAGAAGAAG TAGAGGGTCT GCGCAGAGCC GCAGAACGCT TTAATAAAAT GAAAGAAGAC 
CTGGTGGGAT TGGAAATTGA GAAAGAACAA CTGCTAAAGA AGCTGAAACT TTGGGAGAGC 
CTTGAGCATA CAACTGGATT AAACATAAGT GCAAGAATGC TGGAAACATC ACGGCAGCAG 
CTGCGAGAAG AACTTTTAAA AGTCCAGGCC GGTTTTCTAG AAGAAAAGAA AAGGAGAGAG 
CATCAGGAGG CATTTGTACG CCGTTTGCAA AAGCGGGTCC TTCTTCTGAC TAAGGAACGG 
GATGGAATGA GAGCCATTTT GGATTCTTAT GATAGTGAGT TGACCCCGAC CGAACATTCT 
CCTCAACTGA GCCGCCGCCT GCGGGAAGCA GAAGATATGG TACAGAAGGT TCAGGACCAC 
AATGCTGAAA TGGAGACACA GTTGTCAGAG GCGCTGGAAG AAACAGGCAT TCAGAAGCAG 
AAGTCTGATC TTTTAATGGC AGAGCTGAAA GTCTTAAAGT CTCAGATGGG CTCATCAGAT 
CAAAACATCT CTTTTACAAA TGAGGCAATG AACACTCTCA GATTGAAAAT AGAGGAGCTT 
GAAGCTGAAC GTGGTAGATT AGAGGAAGAG AACAAAATCT TAGAGATGAG ACTGGAACGC 
TTGAATCTTC AGGGATGCTA CGACCCAAGC AAAACAAAAG TGATCCACTT GGGTCTAAAT 
CCCTCAAGTC AAGCCAAGCA GCAACGTGCG GAAACAGTGC GCCAATTACA GGAAGAATGC 
AGTAAGCTGC GGGAGCTTGT AAGGATTTTA GAGGGAGGAG CCCAAATCCC AGACAAACTG 
GAGGCAGCTG GAAGTGTGCA GTCTTCCCAG GAACTTACAG AACTGAAGAA GCAGTTGGAG 
AGTGCTGAAT TGAAAAACCA GCGTCTTCGG GAGGTCTTCC AGACGAAAAT TCATGAGTTC 
CGTACCGCGT GCTACATGCT GACAGGGTAT CGCATTGATA TCACCACTGA GAATCAGTAC 
CGCCTTACAT CCATGTATGC GGAACAGAAA GAAGATAACT TGTTGTTCAA GGCGTCCGGC 
TCCTCCGGAG GGAAGATGCA GCTGCTAGAG ACGGACTTCT CTTTGACCTT GCGTGATTTT 
ATTGATCTTC ACCTACATCA CCAGAACAGC ATCCCAGCAT TCCTCAGTGC AGTCACCCTT 
GACCTCTTCA GTCGCCAGAC CTTTGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mad1l1 ( XM_031892607.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748467.1 Xenopus tropicalis mitotic arrest deficient 1 like 1 (mad1l1), transcript variant X4, mRNA.

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CloneID	OXa65988
Clone ID Related Accession (Same CDS sequence)	XM_031892607.1
Accession Version	XM_031892607.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitotic spindle assembly checkpoint protein MAD1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACA GTGAAGACAA CACCACTGTC ATTTCCACAC TACGGTCATT TAATAAATTT 
CTTTCTCAGC CATTGGAAGG GACAGCACCT TCTCTTGGTA CTTCAACATC TACTGGAGCT 
TCCCTTCAAA TGCAGTTTCA GCAGCGCTTT CTGCTGGAAG ACCAGGCAGC GCAGATTCGT 
TCAAAGTCTC ATCTTATTCA GGTTGAACGT GAAAAGATGC AGATGGAACT AAGCCACAAA 
CGGGCCCGTA TTGAGCTTGA GAAAGCAGCC AGCACCAACG CCAAGAATTA CGAGCGGGAA 
GCAGACAGGA ACCAGGAGCT CATCACCCGC ATCAAGGCTC TAGAGGAAAG GGAGAATGAT 
TTTCAGAACA AACTTCAAGA GCAGAATGAA ATGATCAAAT CCTACAAGAA AACCATAGAG 
GCTCAAAGCA AGAAGCTGCT GGAGAAAGAG GACAAACTGT CTGAATTAAA TGAGAACATC 
AGTGTGTTAA AAGGAAAAGC AACTGAGCTT CAGTGGAAGA TAATGAACCA AGAAATGCAA 
ATAAAGACCC AGGAGACTGA GAAGCAGGAG ATGACAGAGC AGCTGGAAAT CCATCGCAAA 
AAGCTGCAAG AAAGCAATGA GAAGATGCAG GCACTGCATG AGCTTCAGGC GCAAAATGTT 
GATAATGAAC AGAAAATCAA GAGCCTGGAA CAGAAACTGT CAGCCCAGGA ACAGGATGCT 
GCCATTGTGA AGAGCATGAA ATCTGATTTG GCCAAATTGC CGAAACTGGA AAGGGAATTA 
CAGCAGCTCA GAGATGAGAA TGCTTACCAC AGAGAAATGA AAGAAAACAA TGCATTGCTT 
AAAGAAGAAG TAGAGGGTCT GCGCAGAGCC GCAGAACGCT TTAATAAAAT GAAAGAAGAC 
CTGGTGGGAT TGGAAATTGA GAAAGAACAA CTGCTAAAGA AGCTGAAACT TTGGGAGAGC 
CTTGAGCATA CAACTGGATT AAACATAAGA ACTCCGGATG ATCTTTCCAG ACAAATCATT 
GCAGTGCAAC AGAGAGAGCT GAAGCTGAAA GAGGAGAACA TGACAATTCA GAACAGTGCA 
AGAATGCTGG AAACATCACG GCAGCAGCTG CGAGAAGAAC TTTTAAAAGT CCAGGCCGGT 
TTTCTAGAAG AAAAGAAAAG GAGAGAGCAT CAGGAGGCAT TTGTACGCCG TTTGCAAAAG 
CGGGTCCTTC TTCTGACTAA GGAACGGGAT GGAATGAGAG CCATTTTGGA TTCTTATGAT 
AGTGAGTTGA CCCCGACCGA ACATTCTCCT CAACTGAGCC GCCGCCTGCG GGAAGCAGAA 
GATATGGTAC AGAAGGTTCA GGACCACAAT GCTGAAATGG AGACACAGTT GTCAGAGGCG 
CTGGAAGAAA CAGGCATTCA GAAGCAGAAG TCTGATCTTT TAATGGCAGA GCTGAAAGTC 
TTAAAGTCTC AGATGGGCTC ATCAGATCAA AACATCTCTT TTACAAATGA GGCAATGAAC 
ACTCTCAGAT TGAAAATAGA GGAGCTTGAA GCTGAACGTG GTAGATTAGA GGAAGAGAAC 
AAAATCTTAG AGATGAGACT GGAACGCTTG AATCTTCAGG GATGCTACGA CCCAAGCAAA 
ACAAAAGTGA TCCACTTGGG TCTAAATCCC TCAAGTCAAG CCAAGCAGCA ACGTGCGGAA 
ACAGTGCGCC AATTACAGGA AGAATGCAGT AAGCTGCGGG AGCTTGTAAG GATTTTAGAG 
GGAGGAGCCC AAATCCCAGA CAAACTGGAG GCAGCTGGAA GTGTGCAGTC TTCCCAGGAA 
CTTACAGAAC TGAAGAAGCA GTTGGAGAGT GCTGAATTGA AAAACCAGCG TCTTCGGGAG 
GTCTTCCAGA CGAAAATTCA TGAGTTCCGT ACCGCGTGCT ACATGCTGAC AGGGTATCGC 
ATTGATATCA CCACTGAGAA TCAGTACCGC CTTACATCCA TGTATGCGGA ACAGAAAGAA 
GATAACTTGT TGTTCAAGGC ATATTCTGAG CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748467.1
CDS	210..2225
Translation	MDDSEDNTTVISTLRSFNKFLSQPLEGTAPSLGTSTSTGASLQMQFQQRFLLEDQAAQIRSKSHLIQVEREKMQMELSHKRARIELEKAASTNAKNYEREADRNQELITRIKALEERENDFQNKLQEQNEMIKSYKKTIEAQSKKLLEKEDKLSELNENISVLKGKATELQWKIMNQEMQIKTQETEKQEMTEQLEIHRKKLQESNEKMQALHELQAQNVDNEQKIKSLEQKLSAQEQDAAIVKSMKSDLAKLPKLERELQQLRDENAYHREMKENNALLKEEVEGLRRAAERFNKMKEDLVGLEIEKEQLLKKLKLWESLEHTTGLNIRTPDDLSRQIIAVQQRELKLKEENMTIQNSARMLETSRQQLREELLKVQAGFLEEKKRREHQEAFVRRLQKRVLLLTKERDGMRAILDSYDSELTPTEHSPQLSRRLREAEDMVQKVQDHNAEMETQLSEALEETGIQKQKSDLLMAELKVLKSQMGSSDQNISFTNEAMNTLRLKIEELEAERGRLEEENKILEMRLERLNLQGCYDPSKTKVIHLGLNPSSQAKQQRAETVRQLQEECSKLRELVRILEGGAQIPDKLEAAGSVQSSQELTELKKQLESAELKNQRLREVFQTKIHEFRTACYMLTGYRIDITTENQYRLTSMYAEQKEDNLLFKAYSEQ

Target ORF information:

RefSeq Version	XM_031892607.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitotic arrest deficient 1 like 1 (mad1l1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892607.1

ORF Insert Sequence:

ATGGATGACA GTGAAGACAA CACCACTGTC ATTTCCACAC TACGGTCATT TAATAAATTT 
CTTTCTCAGC CATTGGAAGG GACAGCACCT TCTCTTGGTA CTTCAACATC TACTGGAGCT 
TCCCTTCAAA TGCAGTTTCA GCAGCGCTTT CTGCTGGAAG ACCAGGCAGC GCAGATTCGT 
TCAAAGTCTC ATCTTATTCA GGTTGAACGT GAAAAGATGC AGATGGAACT AAGCCACAAA 
CGGGCCCGTA TTGAGCTTGA GAAAGCAGCC AGCACCAACG CCAAGAATTA CGAGCGGGAA 
GCAGACAGGA ACCAGGAGCT CATCACCCGC ATCAAGGCTC TAGAGGAAAG GGAGAATGAT 
TTTCAGAACA AACTTCAAGA GCAGAATGAA ATGATCAAAT CCTACAAGAA AACCATAGAG 
GCTCAAAGCA AGAAGCTGCT GGAGAAAGAG GACAAACTGT CTGAATTAAA TGAGAACATC 
AGTGTGTTAA AAGGAAAAGC AACTGAGCTT CAGTGGAAGA TAATGAACCA AGAAATGCAA 
ATAAAGACCC AGGAGACTGA GAAGCAGGAG ATGACAGAGC AGCTGGAAAT CCATCGCAAA 
AAGCTGCAAG AAAGCAATGA GAAGATGCAG GCACTGCATG AGCTTCAGGC GCAAAATGTT 
GATAATGAAC AGAAAATCAA GAGCCTGGAA CAGAAACTGT CAGCCCAGGA ACAGGATGCT 
GCCATTGTGA AGAGCATGAA ATCTGATTTG GCCAAATTGC CGAAACTGGA AAGGGAATTA 
CAGCAGCTCA GAGATGAGAA TGCTTACCAC AGAGAAATGA AAGAAAACAA TGCATTGCTT 
AAAGAAGAAG TAGAGGGTCT GCGCAGAGCC GCAGAACGCT TTAATAAAAT GAAAGAAGAC 
CTGGTGGGAT TGGAAATTGA GAAAGAACAA CTGCTAAAGA AGCTGAAACT TTGGGAGAGC 
CTTGAGCATA CAACTGGATT AAACATAAGA ACTCCGGATG ATCTTTCCAG ACAAATCATT 
GCAGTGCAAC AGAGAGAGCT GAAGCTGAAA GAGGAGAACA TGACAATTCA GAACAGTGCA 
AGAATGCTGG AAACATCACG GCAGCAGCTG CGAGAAGAAC TTTTAAAAGT CCAGGCCGGT 
TTTCTAGAAG AAAAGAAAAG GAGAGAGCAT CAGGAGGCAT TTGTACGCCG TTTGCAAAAG 
CGGGTCCTTC TTCTGACTAA GGAACGGGAT GGAATGAGAG CCATTTTGGA TTCTTATGAT 
AGTGAGTTGA CCCCGACCGA ACATTCTCCT CAACTGAGCC GCCGCCTGCG GGAAGCAGAA 
GATATGGTAC AGAAGGTTCA GGACCACAAT GCTGAAATGG AGACACAGTT GTCAGAGGCG 
CTGGAAGAAA CAGGCATTCA GAAGCAGAAG TCTGATCTTT TAATGGCAGA GCTGAAAGTC 
TTAAAGTCTC AGATGGGCTC ATCAGATCAA AACATCTCTT TTACAAATGA GGCAATGAAC 
ACTCTCAGAT TGAAAATAGA GGAGCTTGAA GCTGAACGTG GTAGATTAGA GGAAGAGAAC 
AAAATCTTAG AGATGAGACT GGAACGCTTG AATCTTCAGG GATGCTACGA CCCAAGCAAA 
ACAAAAGTGA TCCACTTGGG TCTAAATCCC TCAAGTCAAG CCAAGCAGCA ACGTGCGGAA 
ACAGTGCGCC AATTACAGGA AGAATGCAGT AAGCTGCGGG AGCTTGTAAG GATTTTAGAG 
GGAGGAGCCC AAATCCCAGA CAAACTGGAG GCAGCTGGAA GTGTGCAGTC TTCCCAGGAA 
CTTACAGAAC TGAAGAAGCA GTTGGAGAGT GCTGAATTGA AAAACCAGCG TCTTCGGGAG 
GTCTTCCAGA CGAAAATTCA TGAGTTCCGT ACCGCGTGCT ACATGCTGAC AGGGTATCGC 
ATTGATATCA CCACTGAGAA TCAGTACCGC CTTACATCCA TGTATGCGGA ACAGAAAGAA 
GATAACTTGT TGTTCAAGGC ATATTCTGAG CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mad1l1 ( XM_031892608.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748468.1 Xenopus tropicalis mitotic arrest deficient 1 like 1 (mad1l1), transcript variant X5, mRNA.

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CloneID	OXa65989
Clone ID Related Accession (Same CDS sequence)	XM_031892608.1
Accession Version	XM_031892608.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitotic spindle assembly checkpoint protein MAD1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACA GTGAAGACAA CACCACTGTC ATTTCCACAC TACGGTCATT TAATAAATTT 
CTTTCTCAGC CATTGGAAGG GACAGCACCT TCTCTTGGTA CTTCAACATC TACTGGAGCT 
TCCCTTCAAA TGCAGTTTCA GCAGCGCTTT CTGCTGGAAG ACCAGGCAGC GCAGATTCGT 
TCAAAGTCTC ATCTTATTCA GGTTGAACGT GAAAAGATGC AGATGGAACT AAGCCACAAA 
CGGGCCCGTA TTGAGCTTGA GAAAGCAGCC AGCACCAACG CCAAGAATTA CGAGCGGGAA 
GCAGACAGGA ACCAGGAGCT CATCACCCGC ATCAAGGCTC TAGAGGAAAG GGAGAATGAT 
TTTCAGAACA AACTTCAAGA GCAGAATGAA ATGATCAAAT CCTACAAGAA AACCATAGAG 
GCTCAAAGCA AGAAGCTGCT GGAGAAAGAG GACAAACTGT CTGAATTAAA TGAGAACATC 
AGTGTGTTAA AAGGAAAAGC AACTGAGCTT CAGTGGAAGA TAATGAACCA AGAAATGCAA 
ATAAAGACCC AGGAGACTGA GAAGCAGGAG ATGACAGAGC AGCTGGAAAT CCATCGCAAA 
AAGCTGCAAG AAAGCAATGA GAAGATGCAG GCACTGCATG AGCTTCAGGC GCAAAATGTT 
GATAATGAAC AGAAAATCAA GAGCCTGGAA CAGAAACTGT CAGCCCAGGA ACAGGATGCT 
GCCATTGTGA AGAGCATGAA ATCTGATTTG GCCAAATTGC CGAAACTGGA AAGGGAATTA 
CAGCAGCTCA GAGATGAGAA TGCTTACCAC AGAGAAATGA AAGAAAACAA TGCATTGCTT 
AAAGAAGAAG TAGAGGGTCT GCGCAGAGCC GCAGAACGCT TTAATAAAAT GAAAGAAGAC 
CTGGTGGGAT TGGAAATTGA GAAAGAACAA CTGCTAAAGA AGCTGAAACT TTGGGAGAGC 
CTTGAGCATA CAACTGGATT AAACATAAGA ACTCCGGATG ATCTTTCCAG ACAAATCATT 
GCAGTGCAAC AGAGAGAGCT GAAGCTGAAA GAGGAGAACA TGACAATTCA GAACAGTGCA 
AGAATGCTGG AAACATCACG GCAGCAGCTG CGAGAAGAAC TTTTAAAAGT CCAGGCCGGT 
TTTCTAGAAG AAAAGAAAAG GAGAGAGCAT CAGGAGGCAT TTGTACGCCG TTTGCAAAAG 
CGGGTCCTTC TTCTGACTAA GGAACGGGAT GGAATGAGAG CCATTTTGGA TTCTTATGAT 
AGTGAGTTGA CCCCGACCGA ACATTCTCCT CAACTGAGCC GCCGCCTGCG GGAAGCAGAA 
GATATGGTAC AGAAGGTTCA GGACCACAAT GCTGAAATGG AGACACAGTT GTCAGAGGCG 
CTGGAAGAAA CAGGCATTCA GAAGCAGAAG TCTGATCTTT TAATGGCAGA GCTGAAAGTC 
TTAAAGTCTC AGATGGGCTC ATCAGATCAA AACATCTCTT TTACAAATGA GGCAATGAAC 
ACTCTCAGAT TGAAAATAGA GGAGCTTGAA GCTGAACGTG GTAGATTAGA GGAAGAGAAC 
AAAATCTTAG AGATGAGACT GGAACGCTTG AATCTTCAGG GATGCTACGA CCCAAGCAAA 
ACAAAAGTGA TCCACTTGGG TCTAAATCCC TCAAGTCAAG CCAAGCAGCA ACGTGCGGAA 
ACAGTGCGCC AATTACAGGA AGAATGCAGT AAGCTGCGGG AGCTTGTAAG GATTTTAGAG 
GGAGGAGCCC AAATCCCAGA CAAACTGGAG GCAGCTGGAA GTGTGCAGTC TTCCCAGGAA 
CTTACAGAAC TGAAGAAGCA GTTGGAGAGT GCTGAATTGA AAAACCAGCG TCTTCGGGAG 
GTCTTCCAGA CGAAAATTCA TGAGTTCCGT ACCGCGTGCT ACATGCTGAC AGGGTATCGC 
ATTGATATCA CCACTGAGAA TCAGTACCGC CTTACATCCA TGTATGCGGA ACAGAAAGAA 
GATAACTTGT TGTTCAAGAC GCTGCTCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748468.1
CDS	210..2222
Translation	MDDSEDNTTVISTLRSFNKFLSQPLEGTAPSLGTSTSTGASLQMQFQQRFLLEDQAAQIRSKSHLIQVEREKMQMELSHKRARIELEKAASTNAKNYEREADRNQELITRIKALEERENDFQNKLQEQNEMIKSYKKTIEAQSKKLLEKEDKLSELNENISVLKGKATELQWKIMNQEMQIKTQETEKQEMTEQLEIHRKKLQESNEKMQALHELQAQNVDNEQKIKSLEQKLSAQEQDAAIVKSMKSDLAKLPKLERELQQLRDENAYHREMKENNALLKEEVEGLRRAAERFNKMKEDLVGLEIEKEQLLKKLKLWESLEHTTGLNIRTPDDLSRQIIAVQQRELKLKEENMTIQNSARMLETSRQQLREELLKVQAGFLEEKKRREHQEAFVRRLQKRVLLLTKERDGMRAILDSYDSELTPTEHSPQLSRRLREAEDMVQKVQDHNAEMETQLSEALEETGIQKQKSDLLMAELKVLKSQMGSSDQNISFTNEAMNTLRLKIEELEAERGRLEEENKILEMRLERLNLQGCYDPSKTKVIHLGLNPSSQAKQQRAETVRQLQEECSKLRELVRILEGGAQIPDKLEAAGSVQSSQELTELKKQLESAELKNQRLREVFQTKIHEFRTACYMLTGYRIDITTENQYRLTSMYAEQKEDNLLFKTLLC

Target ORF information:

RefSeq Version	XM_031892608.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitotic arrest deficient 1 like 1 (mad1l1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892608.1

ORF Insert Sequence:

ATGGATGACA GTGAAGACAA CACCACTGTC ATTTCCACAC TACGGTCATT TAATAAATTT 
CTTTCTCAGC CATTGGAAGG GACAGCACCT TCTCTTGGTA CTTCAACATC TACTGGAGCT 
TCCCTTCAAA TGCAGTTTCA GCAGCGCTTT CTGCTGGAAG ACCAGGCAGC GCAGATTCGT 
TCAAAGTCTC ATCTTATTCA GGTTGAACGT GAAAAGATGC AGATGGAACT AAGCCACAAA 
CGGGCCCGTA TTGAGCTTGA GAAAGCAGCC AGCACCAACG CCAAGAATTA CGAGCGGGAA 
GCAGACAGGA ACCAGGAGCT CATCACCCGC ATCAAGGCTC TAGAGGAAAG GGAGAATGAT 
TTTCAGAACA AACTTCAAGA GCAGAATGAA ATGATCAAAT CCTACAAGAA AACCATAGAG 
GCTCAAAGCA AGAAGCTGCT GGAGAAAGAG GACAAACTGT CTGAATTAAA TGAGAACATC 
AGTGTGTTAA AAGGAAAAGC AACTGAGCTT CAGTGGAAGA TAATGAACCA AGAAATGCAA 
ATAAAGACCC AGGAGACTGA GAAGCAGGAG ATGACAGAGC AGCTGGAAAT CCATCGCAAA 
AAGCTGCAAG AAAGCAATGA GAAGATGCAG GCACTGCATG AGCTTCAGGC GCAAAATGTT 
GATAATGAAC AGAAAATCAA GAGCCTGGAA CAGAAACTGT CAGCCCAGGA ACAGGATGCT 
GCCATTGTGA AGAGCATGAA ATCTGATTTG GCCAAATTGC CGAAACTGGA AAGGGAATTA 
CAGCAGCTCA GAGATGAGAA TGCTTACCAC AGAGAAATGA AAGAAAACAA TGCATTGCTT 
AAAGAAGAAG TAGAGGGTCT GCGCAGAGCC GCAGAACGCT TTAATAAAAT GAAAGAAGAC 
CTGGTGGGAT TGGAAATTGA GAAAGAACAA CTGCTAAAGA AGCTGAAACT TTGGGAGAGC 
CTTGAGCATA CAACTGGATT AAACATAAGA ACTCCGGATG ATCTTTCCAG ACAAATCATT 
GCAGTGCAAC AGAGAGAGCT GAAGCTGAAA GAGGAGAACA TGACAATTCA GAACAGTGCA 
AGAATGCTGG AAACATCACG GCAGCAGCTG CGAGAAGAAC TTTTAAAAGT CCAGGCCGGT 
TTTCTAGAAG AAAAGAAAAG GAGAGAGCAT CAGGAGGCAT TTGTACGCCG TTTGCAAAAG 
CGGGTCCTTC TTCTGACTAA GGAACGGGAT GGAATGAGAG CCATTTTGGA TTCTTATGAT 
AGTGAGTTGA CCCCGACCGA ACATTCTCCT CAACTGAGCC GCCGCCTGCG GGAAGCAGAA 
GATATGGTAC AGAAGGTTCA GGACCACAAT GCTGAAATGG AGACACAGTT GTCAGAGGCG 
CTGGAAGAAA CAGGCATTCA GAAGCAGAAG TCTGATCTTT TAATGGCAGA GCTGAAAGTC 
TTAAAGTCTC AGATGGGCTC ATCAGATCAA AACATCTCTT TTACAAATGA GGCAATGAAC 
ACTCTCAGAT TGAAAATAGA GGAGCTTGAA GCTGAACGTG GTAGATTAGA GGAAGAGAAC 
AAAATCTTAG AGATGAGACT GGAACGCTTG AATCTTCAGG GATGCTACGA CCCAAGCAAA 
ACAAAAGTGA TCCACTTGGG TCTAAATCCC TCAAGTCAAG CCAAGCAGCA ACGTGCGGAA 
ACAGTGCGCC AATTACAGGA AGAATGCAGT AAGCTGCGGG AGCTTGTAAG GATTTTAGAG 
GGAGGAGCCC AAATCCCAGA CAAACTGGAG GCAGCTGGAA GTGTGCAGTC TTCCCAGGAA 
CTTACAGAAC TGAAGAAGCA GTTGGAGAGT GCTGAATTGA AAAACCAGCG TCTTCGGGAG 
GTCTTCCAGA CGAAAATTCA TGAGTTCCGT ACCGCGTGCT ACATGCTGAC AGGGTATCGC 
ATTGATATCA CCACTGAGAA TCAGTACCGC CTTACATCCA TGTATGCGGA ACAGAAAGAA 
GATAACTTGT TGTTCAAGAC GCTGCTCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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mad1l1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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