pola2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	pola2
Entrez Gene ID	448613
Full Name	polymerase (DNA directed), alpha 2, accessory subunit
General protein information	Preferred Names polymerase (DNA directed), alpha 2, accessory subunit Names DNA polymerase alpha subunit B polymerase (DNA directed), alpha 2 (70kD subunit)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012960868.2	XP_012816322.1	DNA polymerase alpha subunit B isoform X1
XM_012960869.2	XP_012816323.1	DNA polymerase alpha subunit B isoform X2
NM_001005060.1	NP_001005060.1	DNA polymerase alpha subunit B
XM_012960870.3	XP_012816324.1	DNA polymerase alpha subunit B isoform X1
XM_012960871.3	XP_012816325.1	DNA polymerase alpha subunit B isoform X1
XM_012960870.2	XP_012816324.1	DNA polymerase alpha subunit B isoform X3
XM_012960871.2	XP_012816325.1	DNA polymerase alpha subunit B isoform X3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pola2	NP_955875.1
Drosophila melanogaster (fruit fly)	DNApol-alpha73	NP_733206.3
Homo sapiens (human)	POLA2	NP_002680.2
	POLA2	XP_001113977.1
Bos taurus (cattle)	POLA2	NP_001019713.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004392	XP_313675.5
Pan troglodytes (chimpanzee)	POLA2	XP_001169495.1
Mus musculus (house mouse)	Pola2	NP_032919.2
Gallus gallus (chicken)	LOC100859759	XP_003643474.2
Oryza sativa (rice)	Os12g0242900	NP_001066483.1
Rattus norvegicus (Norway rat)	Pola2	NP_445932.2
Arabidopsis thaliana (thale cress)	POLA2	NP_176930.2
Canis lupus familiaris (dog)	POLA2	XP_533234.2
Xenopus tropicalis (tropical clawed frog)	pola2	NP_001005060.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pola2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pola2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa20631	NM_001005060.1 Latest version!	Xenopus tropicalis polymerase (DNA directed), alpha 2, accessory subunit (pola2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa26721	XM_012960868.2 Latest version!	Xenopus tropicalis polymerase (DNA directed), alpha 2, accessory subunit (pola2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa26722	XM_012960869.2 Latest version!	Xenopus tropicalis polymerase (DNA directed), alpha 2, accessory subunit (pola2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa20631	XM_012960870.3 Latest version!	Xenopus tropicalis polymerase (DNA directed), alpha 2, accessory subunit (pola2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa20631	XM_012960871.3 Latest version!	Xenopus tropicalis polymerase (DNA directed), alpha 2, accessory subunit (pola2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OXa20631	XM_012960870.2	Xenopus tropicalis polymerase (DNA directed), alpha 2, accessory subunit (pola2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa20631	XM_012960871.2	Xenopus tropicalis polymerase (DNA directed), alpha 2, accessory subunit (pola2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pola2 ( NM_001005060.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001005060.1 Xenopus tropicalis polymerase (DNA directed), alpha 2, accessory subunit (pola2), mRNA.

Price & Availability↑

CloneID	OXa20631
Clone ID Related Accession (Same CDS sequence)	NM_001005060.1 , XM_012960870.3 , XM_012960871.3 , XM_012960870.2 , XM_012960871.2
Accession Version	NM_001005060.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA polymerase alpha subunit B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC076954.1. ##Evidence-Data-START## Transcript exon combination :: BC076954.1, CR942408.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGCCTGTGT CAGCAAAGAG CATTGAAGAG GAGCTAACGG TCTTTGACGT CAACTTTGAG 
GATGATGAAG TTCCAGAGAA AATGGTGGAG CTCTGTACAG TTCACCGTCT TCAGGAAGAG 
GACATGGTCA ATGAATGGAT GGCTTTCAGT ACCACTCGGA ACCTTCCGCT CACAGTCAGC 
AACCTTAATA TACTGGAACA TGAGGTTTTG AACAAGAAAA GTGCCAGACC ACGCCCATCC 
CAGAAGAAAG AGAAACATTG TGGAAACAGG GATTTTAATA CAATTCAAGA ACTTATTGAA 
GTGGAAACAG AAGAAGAAAA TCTCCTGGAT TCATATGCTA CACCTGCTAA AGGATCCCAA 
AAGCGTAACT TGTCTACTCC AGAACATCCT CAGTCTAAAC GCATATCTGT AAACCGAAGC 
CCCCATGTCC TCTTCTCCCC AGCAAGCTTT TCCCCCAGTG CCACCCCGTC TCAAAAGTAT 
GGCTCTCGAA CCAACCGAGG AGAAGTGGTG ACAACCTATG GAGAGTTACA AGGAACTACA 
TGGAATGGTG GCAGTGGAAG AAATACTAGT GTTGAACTGT TCACCTGTCA GGATGAACCA 
CTGACAAAAA TGTATAAGTT CATGTTTCAG AAGTTAATGG ACATCAGAGA AGTGGTTAGC 
AGTAAGATAG AAGAACTTGG TGCATCTCTG AAGAACCATT TCCAAATTGA TGAGTTTACC 
TCTGTTTCTT TCCCAGCTCA AGAGACTGTG ACCGTGCTAG GACAGATTGG ATGTGACAGC 
AACGGCAAGT TAAATTCCAA ATCTGTCATC CTTGAGGGGG ACAGAGAGCA CTCAGCAGGG 
ATGCAGGTTC CAGTTGACTT GTCTGAATTA AAAGAGTATT CTCTGTTTCC AGGACAGGTA 
GTAATCATGG AGGGCACCAA CAGCACCGGC AGGAGGTTTG TCCCTACTAA ACTTTATGAG 
GGGGTTCCAC TTCCTTTTCA CCAGCCCAGC AAAGAATTTG AAGAATGTCC ACAGCAGATG 
GTAATCACAG CGTGCGGACC CTTTACCACA TCAGATACTA TCACCTATGA TGCCCTGAAG 
GATCTTATTG ACGTTCTTAA CAGAGACTGT CCAGATATCT GCATCCTGCT TGGTCCCTTT 
TTGGATGCAA AGCATGAGCA AATAGAGAAC CTGCAACTCA CAGTGACATT TGAAGATGTG 
TTTAAGCGTT GTCTGAAAAT GATTATCGAA GGCACACGTC TTTCGGGGTG CCATCTGGTC 
TTCGTTCCAT CTCTCAGAGA TGTTCATCAT GATCCTGTCT ATCCTCAGCC ACCATTCAGC 
TGTTTTGAAC CGGCCAAAGA AGACAAGGAC AGGGTCCATT TTGTTGCCGA TCCCTGCACG 
TTGTCTGTCA ATGGTGTTAT CTTAGGCATA ACCTCTACAG ACTTGCTCTT TCATATGGGT 
GCAGAGGAAA TAAGCAGCTC TGTGGGGGCT CCGGATCGAT TTAGTCGGAT TCTTAGACAC 
ATTCTGACCC AAAGAAGTTA CTATCCACTG TATCCACCCA ATGAAGAAAT AAATGTTGAC 
TATGAGGCTT TCTACAGCTA CGCCCCTATG CCTGTCACTC CAGACATTTT TATAGTCCCG 
TCTGAGCTTC GCTATTTTAT CAAGGATGTC ACAGGCTGCA TCTGCATTAA CCCTGGGCGG 
CTGACAAAGG GACTGGTGGG CGGCACATAT GCACGCTTTC TGGTCAAAAG TGGAGCCATG 
GGGTCTGAAG GAAAGAGATC TCCTTGTATT TCTGCTCAGG TGGTGAGGAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005060.1
CDS	182..1975
Translation	MPVSAKSIEEELTVFDVNFEDDEVPEKMVELCTVHRLQEEDMVNEWMAFSTTRNLPLTVSNLNILEHEVLNKKSARPRPSQKKEKHCGNRDFNTIQELIEVETEEENLLDSYATPAKGSQKRNLSTPEHPQSKRISVNRSPHVLFSPASFSPSATPSQKYGSRTNRGEVVTTYGELQGTTWNGGSGRNTSVELFTCQDEPLTKMYKFMFQKLMDIREVVSSKIEELGASLKNHFQIDEFTSVSFPAQETVTVLGQIGCDSNGKLNSKSVILEGDREHSAGMQVPVDLSELKEYSLFPGQVVIMEGTNSTGRRFVPTKLYEGVPLPFHQPSKEFEECPQQMVITACGPFTTSDTITYDALKDLIDVLNRDCPDICILLGPFLDAKHEQIENLQLTVTFEDVFKRCLKMIIEGTRLSGCHLVFVPSLRDVHHDPVYPQPPFSCFEPAKEDKDRVHFVADPCTLSVNGVILGITSTDLLFHMGAEEISSSVGAPDRFSRILRHILTQRSYYPLYPPNEEINVDYEAFYSYAPMPVTPDIFIVPSELRYFIKDVTGCICINPGRLTKGLVGGTYARFLVKSGAMGSEGKRSPCISAQVVRI

Target ORF information:

RefSeq Version	NM_001005060.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis polymerase (DNA directed), alpha 2, accessory subunit (pola2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005060.1

ORF Insert Sequence:

ATGCCTGTGT CAGCAAAGAG CATTGAAGAG GAGCTAACGG TCTTTGACGT CAACTTTGAG 
GATGATGAAG TTCCAGAGAA AATGGTGGAG CTCTGTACAG TTCACCGTCT TCAGGAAGAG 
GACATGGTCA ATGAATGGAT GGCTTTCAGT ACCACTCGGA ACCTTCCGCT CACAGTCAGC 
AACCTTAATA TACTGGAACA TGAGGTTTTG AACAAGAAAA GTGCCAGACC ACGCCCATCC 
CAGAAGAAAG AGAAACATTG TGGAAACAGG GATTTTAATA CAATTCAAGA ACTTATTGAA 
GTGGAAACAG AAGAAGAAAA TCTCCTGGAT TCATATGCTA CACCTGCTAA AGGATCCCAA 
AAGCGTAACT TGTCTACTCC AGAACATCCT CAGTCTAAAC GCATATCTGT AAACCGAAGC 
CCCCATGTCC TCTTCTCCCC AGCAAGCTTT TCCCCCAGTG CCACCCCGTC TCAAAAGTAT 
GGCTCTCGAA CCAACCGAGG AGAAGTGGTG ACAACCTATG GAGAGTTACA AGGAACTACA 
TGGAATGGTG GCAGTGGAAG AAATACTAGT GTTGAACTGT TCACCTGTCA GGATGAACCA 
CTGACAAAAA TGTATAAGTT CATGTTTCAG AAGTTAATGG ACATCAGAGA AGTGGTTAGC 
AGTAAGATAG AAGAACTTGG TGCATCTCTG AAGAACCATT TCCAAATTGA TGAGTTTACC 
TCTGTTTCTT TCCCAGCTCA AGAGACTGTG ACCGTGCTAG GACAGATTGG ATGTGACAGC 
AACGGCAAGT TAAATTCCAA ATCTGTCATC CTTGAGGGGG ACAGAGAGCA CTCAGCAGGG 
ATGCAGGTTC CAGTTGACTT GTCTGAATTA AAAGAGTATT CTCTGTTTCC AGGACAGGTA 
GTAATCATGG AGGGCACCAA CAGCACCGGC AGGAGGTTTG TCCCTACTAA ACTTTATGAG 
GGGGTTCCAC TTCCTTTTCA CCAGCCCAGC AAAGAATTTG AAGAATGTCC ACAGCAGATG 
GTAATCACAG CGTGCGGACC CTTTACCACA TCAGATACTA TCACCTATGA TGCCCTGAAG 
GATCTTATTG ACGTTCTTAA CAGAGACTGT CCAGATATCT GCATCCTGCT TGGTCCCTTT 
TTGGATGCAA AGCATGAGCA AATAGAGAAC CTGCAACTCA CAGTGACATT TGAAGATGTG 
TTTAAGCGTT GTCTGAAAAT GATTATCGAA GGCACACGTC TTTCGGGGTG CCATCTGGTC 
TTCGTTCCAT CTCTCAGAGA TGTTCATCAT GATCCTGTCT ATCCTCAGCC ACCATTCAGC 
TGTTTTGAAC CGGCCAAAGA AGACAAGGAC AGGGTCCATT TTGTTGCCGA TCCCTGCACG 
TTGTCTGTCA ATGGTGTTAT CTTAGGCATA ACCTCTACAG ACTTGCTCTT TCATATGGGT 
GCAGAGGAAA TAAGCAGCTC TGTGGGGGCT CCGGATCGAT TTAGTCGGAT TCTTAGACAC 
ATTCTGACCC AAAGAAGTTA CTATCCACTG TATCCACCCA ATGAAGAAAT AAATGTTGAC 
TATGAGGCTT TCTACAGCTA CGCCCCTATG CCTGTCACTC CAGACATTTT TATAGTCCCG 
TCTGAGCTTC GCTATTTTAT CAAGGATGTC ACAGGCTGCA TCTGCATTAA CCCTGGGCGG 
CTGACAAAGG GACTGGTGGG CGGCACATAT GCACGCTTTC TGGTCAAAAG TGGAGCCATG 
GGGTCTGAAG GAAAGAGATC TCCTTGTATT TCTGCTCAGG TGGTGAGGAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pola2 ( XM_012960870.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816324.1 Xenopus tropicalis polymerase (DNA directed), alpha 2, accessory subunit (pola2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa20631
Clone ID Related Accession (Same CDS sequence)	NM_001005060.1 , XM_012960870.3 , XM_012960871.3 , XM_012960870.2 , XM_012960871.2
Accession Version	XM_012960870.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA polymerase alpha subunit B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960870.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGT CAGCAAAGAG CATTGAAGAG GAGCTAACGG TCTTTGACGT CAACTTTGAG 
GATGATGAAG TTCCAGAGAA AATGGTGGAG CTCTGTACAG TTCACCGTCT TCAGGAAGAG 
GACATGGTCA ATGAATGGAT GGCTTTCAGT ACCACTCGGA ACCTTCCGCT CACAGTCAGC 
AACCTTAATA TACTGGAACA TGAGGTTTTG AACAAGAAAA GTGCCAGACC ACGCCCATCC 
CAGAAGAAAG AGAAACATTG TGGAAACAGG GATTTTAATA CAATTCAAGA ACTTATTGAA 
GTGGAAACAG AAGAAGAAAA TCTCCTGGAT TCATATGCTA CACCTGCTAA AGGATCCCAA 
AAGCGTAACT TGTCTACTCC AGAACATCCT CAGTCTAAAC GCATATCTGT AAACCGAAGC 
CCCCATGTCC TCTTCTCCCC AGCAAGCTTT TCCCCCAGTG CCACCCCGTC TCAAAAGTAT 
GGCTCTCGAA CCAACCGAGG AGAAGTGGTG ACAACCTATG GAGAGTTACA AGGAACTACA 
TGGAATGGTG GCAGTGGAAG AAATACTAGT GTTGAACTGT TCACCTGTCA GGATGAACCA 
CTGACAAAAA TGTATAAGTT CATGTTTCAG AAGTTAATGG ACATCAGAGA AGTGGTTAGC 
AGTAAGATAG AAGAACTTGG TGCATCTCTG AAGAACCATT TCCAAATTGA TGAGTTTACC 
TCTGTTTCTT TCCCAGCTCA AGAGACTGTG ACCGTGCTAG GACAGATTGG ATGTGACAGC 
AACGGCAAGT TAAATTCCAA ATCTGTCATC CTTGAGGGGG ACAGAGAGCA CTCAGCAGGG 
ATGCAGGTTC CAGTTGACTT GTCTGAATTA AAAGAGTATT CTCTGTTTCC AGGACAGGTA 
GTAATCATGG AGGGCACCAA CAGCACCGGC AGGAGGTTTG TCCCTACTAA ACTTTATGAG 
GGGGTTCCAC TTCCTTTTCA CCAGCCCAGC AAAGAATTTG AAGAATGTCC ACAGCAGATG 
GTAATCACAG CGTGCGGACC CTTTACCACA TCAGATACTA TCACCTATGA TGCCCTGAAG 
GATCTTATTG ACGTTCTTAA CAGAGACTGT CCAGATATCT GCATCCTGCT TGGTCCCTTT 
TTGGATGCAA AGCATGAGCA AATAGAGAAC CTGCAACTCA CAGTGACATT TGAAGATGTG 
TTTAAGCGTT GTCTGAAAAT GATTATCGAA GGCACACGTC TTTCGGGGTG CCATCTGGTC 
TTCGTTCCAT CTCTCAGAGA TGTTCATCAT GATCCTGTCT ATCCTCAGCC ACCATTCAGC 
TGTTTTGAAC CGGCCAAAGA AGACAAGGAC AGGGTCCATT TTGTTGCCGA TCCCTGCACG 
TTGTCTGTCA ATGGTGTTAT CTTAGGCATA ACCTCTACAG ACTTGCTCTT TCATATGGGT 
GCAGAGGAAA TAAGCAGCTC TGTGGGGGCT CCGGATCGAT TTAGTCGGAT TCTTAGACAC 
ATTCTGACCC AAAGAAGTTA CTATCCACTG TATCCACCCA ATGAAGAAAT AAATGTTGAC 
TATGAGGCTT TCTACAGCTA CGCCCCTATG CCTGTCACTC CAGACATTTT TATAGTCCCG 
TCTGAGCTTC GCTATTTTAT CAAGGATGTC ACAGGCTGCA TCTGCATTAA CCCTGGGCGG 
CTGACAAAGG GACTGGTGGG CGGCACATAT GCACGCTTTC TGGTCAAAAG TGGAGCCATG 
GGGTCTGAAG GAAAGAGATC TCCTTGTATT TCTGCTCAGG TGGTGAGGAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816324.1
CDS	261..2054
Translation	MPVSAKSIEEELTVFDVNFEDDEVPEKMVELCTVHRLQEEDMVNEWMAFSTTRNLPLTVSNLNILEHEVLNKKSARPRPSQKKEKHCGNRDFNTIQELIEVETEEENLLDSYATPAKGSQKRNLSTPEHPQSKRISVNRSPHVLFSPASFSPSATPSQKYGSRTNRGEVVTTYGELQGTTWNGGSGRNTSVELFTCQDEPLTKMYKFMFQKLMDIREVVSSKIEELGASLKNHFQIDEFTSVSFPAQETVTVLGQIGCDSNGKLNSKSVILEGDREHSAGMQVPVDLSELKEYSLFPGQVVIMEGTNSTGRRFVPTKLYEGVPLPFHQPSKEFEECPQQMVITACGPFTTSDTITYDALKDLIDVLNRDCPDICILLGPFLDAKHEQIENLQLTVTFEDVFKRCLKMIIEGTRLSGCHLVFVPSLRDVHHDPVYPQPPFSCFEPAKEDKDRVHFVADPCTLSVNGVILGITSTDLLFHMGAEEISSSVGAPDRFSRILRHILTQRSYYPLYPPNEEINVDYEAFYSYAPMPVTPDIFIVPSELRYFIKDVTGCICINPGRLTKGLVGGTYARFLVKSGAMGSEGKRSPCISAQVVRI

Target ORF information:

RefSeq Version	XM_012960870.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis polymerase (DNA directed), alpha 2, accessory subunit (pola2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960870.2

ORF Insert Sequence:

ATGCCTGTGT CAGCAAAGAG CATTGAAGAG GAGCTAACGG TCTTTGACGT CAACTTTGAG 
GATGATGAAG TTCCAGAGAA AATGGTGGAG CTCTGTACAG TTCACCGTCT TCAGGAAGAG 
GACATGGTCA ATGAATGGAT GGCTTTCAGT ACCACTCGGA ACCTTCCGCT CACAGTCAGC 
AACCTTAATA TACTGGAACA TGAGGTTTTG AACAAGAAAA GTGCCAGACC ACGCCCATCC 
CAGAAGAAAG AGAAACATTG TGGAAACAGG GATTTTAATA CAATTCAAGA ACTTATTGAA 
GTGGAAACAG AAGAAGAAAA TCTCCTGGAT TCATATGCTA CACCTGCTAA AGGATCCCAA 
AAGCGTAACT TGTCTACTCC AGAACATCCT CAGTCTAAAC GCATATCTGT AAACCGAAGC 
CCCCATGTCC TCTTCTCCCC AGCAAGCTTT TCCCCCAGTG CCACCCCGTC TCAAAAGTAT 
GGCTCTCGAA CCAACCGAGG AGAAGTGGTG ACAACCTATG GAGAGTTACA AGGAACTACA 
TGGAATGGTG GCAGTGGAAG AAATACTAGT GTTGAACTGT TCACCTGTCA GGATGAACCA 
CTGACAAAAA TGTATAAGTT CATGTTTCAG AAGTTAATGG ACATCAGAGA AGTGGTTAGC 
AGTAAGATAG AAGAACTTGG TGCATCTCTG AAGAACCATT TCCAAATTGA TGAGTTTACC 
TCTGTTTCTT TCCCAGCTCA AGAGACTGTG ACCGTGCTAG GACAGATTGG ATGTGACAGC 
AACGGCAAGT TAAATTCCAA ATCTGTCATC CTTGAGGGGG ACAGAGAGCA CTCAGCAGGG 
ATGCAGGTTC CAGTTGACTT GTCTGAATTA AAAGAGTATT CTCTGTTTCC AGGACAGGTA 
GTAATCATGG AGGGCACCAA CAGCACCGGC AGGAGGTTTG TCCCTACTAA ACTTTATGAG 
GGGGTTCCAC TTCCTTTTCA CCAGCCCAGC AAAGAATTTG AAGAATGTCC ACAGCAGATG 
GTAATCACAG CGTGCGGACC CTTTACCACA TCAGATACTA TCACCTATGA TGCCCTGAAG 
GATCTTATTG ACGTTCTTAA CAGAGACTGT CCAGATATCT GCATCCTGCT TGGTCCCTTT 
TTGGATGCAA AGCATGAGCA AATAGAGAAC CTGCAACTCA CAGTGACATT TGAAGATGTG 
TTTAAGCGTT GTCTGAAAAT GATTATCGAA GGCACACGTC TTTCGGGGTG CCATCTGGTC 
TTCGTTCCAT CTCTCAGAGA TGTTCATCAT GATCCTGTCT ATCCTCAGCC ACCATTCAGC 
TGTTTTGAAC CGGCCAAAGA AGACAAGGAC AGGGTCCATT TTGTTGCCGA TCCCTGCACG 
TTGTCTGTCA ATGGTGTTAT CTTAGGCATA ACCTCTACAG ACTTGCTCTT TCATATGGGT 
GCAGAGGAAA TAAGCAGCTC TGTGGGGGCT CCGGATCGAT TTAGTCGGAT TCTTAGACAC 
ATTCTGACCC AAAGAAGTTA CTATCCACTG TATCCACCCA ATGAAGAAAT AAATGTTGAC 
TATGAGGCTT TCTACAGCTA CGCCCCTATG CCTGTCACTC CAGACATTTT TATAGTCCCG 
TCTGAGCTTC GCTATTTTAT CAAGGATGTC ACAGGCTGCA TCTGCATTAA CCCTGGGCGG 
CTGACAAAGG GACTGGTGGG CGGCACATAT GCACGCTTTC TGGTCAAAAG TGGAGCCATG 
GGGTCTGAAG GAAAGAGATC TCCTTGTATT TCTGCTCAGG TGGTGAGGAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pola2 ( XM_012960871.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816325.1 Xenopus tropicalis polymerase (DNA directed), alpha 2, accessory subunit (pola2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa20631
Clone ID Related Accession (Same CDS sequence)	NM_001005060.1 , XM_012960870.3 , XM_012960871.3 , XM_012960870.2 , XM_012960871.2
Accession Version	XM_012960871.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA polymerase alpha subunit B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960871.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGT CAGCAAAGAG CATTGAAGAG GAGCTAACGG TCTTTGACGT CAACTTTGAG 
GATGATGAAG TTCCAGAGAA AATGGTGGAG CTCTGTACAG TTCACCGTCT TCAGGAAGAG 
GACATGGTCA ATGAATGGAT GGCTTTCAGT ACCACTCGGA ACCTTCCGCT CACAGTCAGC 
AACCTTAATA TACTGGAACA TGAGGTTTTG AACAAGAAAA GTGCCAGACC ACGCCCATCC 
CAGAAGAAAG AGAAACATTG TGGAAACAGG GATTTTAATA CAATTCAAGA ACTTATTGAA 
GTGGAAACAG AAGAAGAAAA TCTCCTGGAT TCATATGCTA CACCTGCTAA AGGATCCCAA 
AAGCGTAACT TGTCTACTCC AGAACATCCT CAGTCTAAAC GCATATCTGT AAACCGAAGC 
CCCCATGTCC TCTTCTCCCC AGCAAGCTTT TCCCCCAGTG CCACCCCGTC TCAAAAGTAT 
GGCTCTCGAA CCAACCGAGG AGAAGTGGTG ACAACCTATG GAGAGTTACA AGGAACTACA 
TGGAATGGTG GCAGTGGAAG AAATACTAGT GTTGAACTGT TCACCTGTCA GGATGAACCA 
CTGACAAAAA TGTATAAGTT CATGTTTCAG AAGTTAATGG ACATCAGAGA AGTGGTTAGC 
AGTAAGATAG AAGAACTTGG TGCATCTCTG AAGAACCATT TCCAAATTGA TGAGTTTACC 
TCTGTTTCTT TCCCAGCTCA AGAGACTGTG ACCGTGCTAG GACAGATTGG ATGTGACAGC 
AACGGCAAGT TAAATTCCAA ATCTGTCATC CTTGAGGGGG ACAGAGAGCA CTCAGCAGGG 
ATGCAGGTTC CAGTTGACTT GTCTGAATTA AAAGAGTATT CTCTGTTTCC AGGACAGGTA 
GTAATCATGG AGGGCACCAA CAGCACCGGC AGGAGGTTTG TCCCTACTAA ACTTTATGAG 
GGGGTTCCAC TTCCTTTTCA CCAGCCCAGC AAAGAATTTG AAGAATGTCC ACAGCAGATG 
GTAATCACAG CGTGCGGACC CTTTACCACA TCAGATACTA TCACCTATGA TGCCCTGAAG 
GATCTTATTG ACGTTCTTAA CAGAGACTGT CCAGATATCT GCATCCTGCT TGGTCCCTTT 
TTGGATGCAA AGCATGAGCA AATAGAGAAC CTGCAACTCA CAGTGACATT TGAAGATGTG 
TTTAAGCGTT GTCTGAAAAT GATTATCGAA GGCACACGTC TTTCGGGGTG CCATCTGGTC 
TTCGTTCCAT CTCTCAGAGA TGTTCATCAT GATCCTGTCT ATCCTCAGCC ACCATTCAGC 
TGTTTTGAAC CGGCCAAAGA AGACAAGGAC AGGGTCCATT TTGTTGCCGA TCCCTGCACG 
TTGTCTGTCA ATGGTGTTAT CTTAGGCATA ACCTCTACAG ACTTGCTCTT TCATATGGGT 
GCAGAGGAAA TAAGCAGCTC TGTGGGGGCT CCGGATCGAT TTAGTCGGAT TCTTAGACAC 
ATTCTGACCC AAAGAAGTTA CTATCCACTG TATCCACCCA ATGAAGAAAT AAATGTTGAC 
TATGAGGCTT TCTACAGCTA CGCCCCTATG CCTGTCACTC CAGACATTTT TATAGTCCCG 
TCTGAGCTTC GCTATTTTAT CAAGGATGTC ACAGGCTGCA TCTGCATTAA CCCTGGGCGG 
CTGACAAAGG GACTGGTGGG CGGCACATAT GCACGCTTTC TGGTCAAAAG TGGAGCCATG 
GGGTCTGAAG GAAAGAGATC TCCTTGTATT TCTGCTCAGG TGGTGAGGAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816325.1
CDS	93..1886
Translation	MPVSAKSIEEELTVFDVNFEDDEVPEKMVELCTVHRLQEEDMVNEWMAFSTTRNLPLTVSNLNILEHEVLNKKSARPRPSQKKEKHCGNRDFNTIQELIEVETEEENLLDSYATPAKGSQKRNLSTPEHPQSKRISVNRSPHVLFSPASFSPSATPSQKYGSRTNRGEVVTTYGELQGTTWNGGSGRNTSVELFTCQDEPLTKMYKFMFQKLMDIREVVSSKIEELGASLKNHFQIDEFTSVSFPAQETVTVLGQIGCDSNGKLNSKSVILEGDREHSAGMQVPVDLSELKEYSLFPGQVVIMEGTNSTGRRFVPTKLYEGVPLPFHQPSKEFEECPQQMVITACGPFTTSDTITYDALKDLIDVLNRDCPDICILLGPFLDAKHEQIENLQLTVTFEDVFKRCLKMIIEGTRLSGCHLVFVPSLRDVHHDPVYPQPPFSCFEPAKEDKDRVHFVADPCTLSVNGVILGITSTDLLFHMGAEEISSSVGAPDRFSRILRHILTQRSYYPLYPPNEEINVDYEAFYSYAPMPVTPDIFIVPSELRYFIKDVTGCICINPGRLTKGLVGGTYARFLVKSGAMGSEGKRSPCISAQVVRI

Target ORF information:

RefSeq Version	XM_012960871.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis polymerase (DNA directed), alpha 2, accessory subunit (pola2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960871.2

ORF Insert Sequence:

ATGCCTGTGT CAGCAAAGAG CATTGAAGAG GAGCTAACGG TCTTTGACGT CAACTTTGAG 
GATGATGAAG TTCCAGAGAA AATGGTGGAG CTCTGTACAG TTCACCGTCT TCAGGAAGAG 
GACATGGTCA ATGAATGGAT GGCTTTCAGT ACCACTCGGA ACCTTCCGCT CACAGTCAGC 
AACCTTAATA TACTGGAACA TGAGGTTTTG AACAAGAAAA GTGCCAGACC ACGCCCATCC 
CAGAAGAAAG AGAAACATTG TGGAAACAGG GATTTTAATA CAATTCAAGA ACTTATTGAA 
GTGGAAACAG AAGAAGAAAA TCTCCTGGAT TCATATGCTA CACCTGCTAA AGGATCCCAA 
AAGCGTAACT TGTCTACTCC AGAACATCCT CAGTCTAAAC GCATATCTGT AAACCGAAGC 
CCCCATGTCC TCTTCTCCCC AGCAAGCTTT TCCCCCAGTG CCACCCCGTC TCAAAAGTAT 
GGCTCTCGAA CCAACCGAGG AGAAGTGGTG ACAACCTATG GAGAGTTACA AGGAACTACA 
TGGAATGGTG GCAGTGGAAG AAATACTAGT GTTGAACTGT TCACCTGTCA GGATGAACCA 
CTGACAAAAA TGTATAAGTT CATGTTTCAG AAGTTAATGG ACATCAGAGA AGTGGTTAGC 
AGTAAGATAG AAGAACTTGG TGCATCTCTG AAGAACCATT TCCAAATTGA TGAGTTTACC 
TCTGTTTCTT TCCCAGCTCA AGAGACTGTG ACCGTGCTAG GACAGATTGG ATGTGACAGC 
AACGGCAAGT TAAATTCCAA ATCTGTCATC CTTGAGGGGG ACAGAGAGCA CTCAGCAGGG 
ATGCAGGTTC CAGTTGACTT GTCTGAATTA AAAGAGTATT CTCTGTTTCC AGGACAGGTA 
GTAATCATGG AGGGCACCAA CAGCACCGGC AGGAGGTTTG TCCCTACTAA ACTTTATGAG 
GGGGTTCCAC TTCCTTTTCA CCAGCCCAGC AAAGAATTTG AAGAATGTCC ACAGCAGATG 
GTAATCACAG CGTGCGGACC CTTTACCACA TCAGATACTA TCACCTATGA TGCCCTGAAG 
GATCTTATTG ACGTTCTTAA CAGAGACTGT CCAGATATCT GCATCCTGCT TGGTCCCTTT 
TTGGATGCAA AGCATGAGCA AATAGAGAAC CTGCAACTCA CAGTGACATT TGAAGATGTG 
TTTAAGCGTT GTCTGAAAAT GATTATCGAA GGCACACGTC TTTCGGGGTG CCATCTGGTC 
TTCGTTCCAT CTCTCAGAGA TGTTCATCAT GATCCTGTCT ATCCTCAGCC ACCATTCAGC 
TGTTTTGAAC CGGCCAAAGA AGACAAGGAC AGGGTCCATT TTGTTGCCGA TCCCTGCACG 
TTGTCTGTCA ATGGTGTTAT CTTAGGCATA ACCTCTACAG ACTTGCTCTT TCATATGGGT 
GCAGAGGAAA TAAGCAGCTC TGTGGGGGCT CCGGATCGAT TTAGTCGGAT TCTTAGACAC 
ATTCTGACCC AAAGAAGTTA CTATCCACTG TATCCACCCA ATGAAGAAAT AAATGTTGAC 
TATGAGGCTT TCTACAGCTA CGCCCCTATG CCTGTCACTC CAGACATTTT TATAGTCCCG 
TCTGAGCTTC GCTATTTTAT CAAGGATGTC ACAGGCTGCA TCTGCATTAA CCCTGGGCGG 
CTGACAAAGG GACTGGTGGG CGGCACATAT GCACGCTTTC TGGTCAAAAG TGGAGCCATG 
GGGTCTGAAG GAAAGAGATC TCCTTGTATT TCTGCTCAGG TGGTGAGGAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pola2 ( XM_012960868.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816322.1 Xenopus tropicalis polymerase (DNA directed), alpha 2, accessory subunit (pola2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa26721
Clone ID Related Accession (Same CDS sequence)	XM_012960868.2
Accession Version	XM_012960868.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA polymerase alpha subunit B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960868.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG GGCCAGTGAG CCTGCAGTTC CCTTGTGAAT GTGGTCAGTG CAGGTCCCAT 
ATACAGATTT GCAAGTACCA GCCTGCCTCC CGCATCAAGG ATCTGCCATC CTTTGAGCAT 
ACAACTGGTG AAGCCATGCC TGTGTCAGCA AAGAGCATTG AAGAGGAGCT AACGGTCTTT 
GACGTCAACT TTGAGGATGA TGAAGTTCCA GAGAAAATGG TGGAGCTCTG TACAGTTCAC 
CGTCTTCAGG AAGAGGACAT GGTCAATGAA TGGATGGCTT TCAGTACCAC TCGGAACCTT 
CCGCTCACAG TCAGCAACCT TAATATACTG GAACATGAGG TTTTGAACAA GAAAAGTGCC 
AGACCACGCC CATCCCAGAA GAAAGAGAAA CATTGTGGAA ACAGGGATTT TAATACAATT 
CAAGAACTTA TTGAAGTGGA AACAGAAGAA GAAAATCTCC TGGATTCATA TGCTACACCT 
GCTAAAGGAT CCCAAAAGCG TAACTTGTCT ACTCCAGAAC ATCCTCAGTC TAAACGCATA 
TCTGTAAACC GAAGCCCCCA TGTCCTCTTC TCCCCAGCAA GCTTTTCCCC CAGTGCCACC 
CCGTCTCAAA AGTATGGCTC TCGAACCAAC CGAGGAGAAG TGGTGACAAC CTATGGAGAG 
TTACAAGGAA CTACATGGAA TGGTGGCAGT GGAAGAAATA CTAGTGTTGA ACTGTTCACC 
TGTCAGGATG AACCACTGAC AAAAATGTAT AAGTTCATGT TTCAGAAGTT AATGGACATC 
AGAGAAGTGG TTAGCAGTAA GATAGAAGAA CTTGGTGCAT CTCTGAAGAA CCATTTCCAA 
ATTGATGAGT TTACCTCTGT TTCTTTCCCA GCTCAAGAGA CTGTGACCGT GCTAGGACAG 
ATTGGATGTG ACAGCAACGG CAAGTTAAAT TCCAAATCTG TCATCCTTGA GGGGGACAGA 
GAGCACTCAG CAGGGATGCA GGTTCCAGTT GACTTGTCTG AATTAAAAGA GTATTCTCTG 
TTTCCAGGAC AGGTAGTAAT CATGGAGGGC ACCAACAGCA CCGGCAGGAG GTTTGTCCCT 
ACTAAACTTT ATGAGGGGGT TCCACTTCCT TTTCACCAGC CCAGCAAAGA ATTTGAAGAA 
TGTCCACAGC AGATGGTAAT CACAGCGTGC GGACCCTTTA CCACATCAGA TACTATCACC 
TATGATGCCC TGAAGGATCT TATTGACGTT CTTAACAGAG ACTGTCCAGA TATCTGCATC 
CTGCTTGGTC CCTTTTTGGA TGCAAAGCAT GAGCAAATAG AGAACCTGCA ACTCACAGTG 
ACATTTGAAG ATGTGTTTAA GCGTTGTCTG AAAATGATTA TCGAAGGCAC ACGTCTTTCG 
GGGTGCCATC TGGTCTTCGT TCCATCTCTC AGAGATGTTC ATCATGATCC TGTCTATCCT 
CAGCCACCAT TCAGCTGTTT TGAACCGGCC AAAGAAGACA AGGACAGGGT CCATTTTGTT 
GCCGATCCCT GCACGTTGTC TGTCAATGGT GTTATCTTAG GCATAACCTC TACAGACTTG 
CTCTTTCATA TGGGTGCAGA GGAAATAAGC AGCTCTGTGG GGGCTCCGGA TCGATTTAGT 
CGGATTCTTA GACACATTCT GACCCAAAGA AGTTACTATC CACTGTATCC ACCCAATGAA 
GAAATAAATG TTGACTATGA GGCTTTCTAC AGCTACGCCC CTATGCCTGT CACTCCAGAC 
ATTTTTATAG TCCCGTCTGA GCTTCGCTAT TTTATCAAGG ATGTCACAGG CTGCATCTGC 
ATTAACCCTG GGCGGCTGAC AAAGGGACTG GTGGGCGGCA CATATGCACG CTTTCTGGTC 
AAAAGTGGAG CCATGGGGTC TGAAGGAAAG AGATCTCCTT GTATTTCTGC TCAGGTGGTG 
AGGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816322.1
CDS	2..1930
Translation	MAPGPVSLQFPCECGQCRSHIQICKYQPASRIKDLPSFEHTTGEAMPVSAKSIEEELTVFDVNFEDDEVPEKMVELCTVHRLQEEDMVNEWMAFSTTRNLPLTVSNLNILEHEVLNKKSARPRPSQKKEKHCGNRDFNTIQELIEVETEEENLLDSYATPAKGSQKRNLSTPEHPQSKRISVNRSPHVLFSPASFSPSATPSQKYGSRTNRGEVVTTYGELQGTTWNGGSGRNTSVELFTCQDEPLTKMYKFMFQKLMDIREVVSSKIEELGASLKNHFQIDEFTSVSFPAQETVTVLGQIGCDSNGKLNSKSVILEGDREHSAGMQVPVDLSELKEYSLFPGQVVIMEGTNSTGRRFVPTKLYEGVPLPFHQPSKEFEECPQQMVITACGPFTTSDTITYDALKDLIDVLNRDCPDICILLGPFLDAKHEQIENLQLTVTFEDVFKRCLKMIIEGTRLSGCHLVFVPSLRDVHHDPVYPQPPFSCFEPAKEDKDRVHFVADPCTLSVNGVILGITSTDLLFHMGAEEISSSVGAPDRFSRILRHILTQRSYYPLYPPNEEINVDYEAFYSYAPMPVTPDIFIVPSELRYFIKDVTGCICINPGRLTKGLVGGTYARFLVKSGAMGSEGKRSPCISAQVVRI

Target ORF information:

RefSeq Version	XM_012960868.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis polymerase (DNA directed), alpha 2, accessory subunit (pola2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960868.2

ORF Insert Sequence:

ATGGCCCCTG GGCCAGTGAG CCTGCAGTTC CCTTGTGAAT GTGGTCAGTG CAGGTCCCAT 
ATACAGATTT GCAAGTACCA GCCTGCCTCC CGCATCAAGG ATCTGCCATC CTTTGAGCAT 
ACAACTGGTG AAGCCATGCC TGTGTCAGCA AAGAGCATTG AAGAGGAGCT AACGGTCTTT 
GACGTCAACT TTGAGGATGA TGAAGTTCCA GAGAAAATGG TGGAGCTCTG TACAGTTCAC 
CGTCTTCAGG AAGAGGACAT GGTCAATGAA TGGATGGCTT TCAGTACCAC TCGGAACCTT 
CCGCTCACAG TCAGCAACCT TAATATACTG GAACATGAGG TTTTGAACAA GAAAAGTGCC 
AGACCACGCC CATCCCAGAA GAAAGAGAAA CATTGTGGAA ACAGGGATTT TAATACAATT 
CAAGAACTTA TTGAAGTGGA AACAGAAGAA GAAAATCTCC TGGATTCATA TGCTACACCT 
GCTAAAGGAT CCCAAAAGCG TAACTTGTCT ACTCCAGAAC ATCCTCAGTC TAAACGCATA 
TCTGTAAACC GAAGCCCCCA TGTCCTCTTC TCCCCAGCAA GCTTTTCCCC CAGTGCCACC 
CCGTCTCAAA AGTATGGCTC TCGAACCAAC CGAGGAGAAG TGGTGACAAC CTATGGAGAG 
TTACAAGGAA CTACATGGAA TGGTGGCAGT GGAAGAAATA CTAGTGTTGA ACTGTTCACC 
TGTCAGGATG AACCACTGAC AAAAATGTAT AAGTTCATGT TTCAGAAGTT AATGGACATC 
AGAGAAGTGG TTAGCAGTAA GATAGAAGAA CTTGGTGCAT CTCTGAAGAA CCATTTCCAA 
ATTGATGAGT TTACCTCTGT TTCTTTCCCA GCTCAAGAGA CTGTGACCGT GCTAGGACAG 
ATTGGATGTG ACAGCAACGG CAAGTTAAAT TCCAAATCTG TCATCCTTGA GGGGGACAGA 
GAGCACTCAG CAGGGATGCA GGTTCCAGTT GACTTGTCTG AATTAAAAGA GTATTCTCTG 
TTTCCAGGAC AGGTAGTAAT CATGGAGGGC ACCAACAGCA CCGGCAGGAG GTTTGTCCCT 
ACTAAACTTT ATGAGGGGGT TCCACTTCCT TTTCACCAGC CCAGCAAAGA ATTTGAAGAA 
TGTCCACAGC AGATGGTAAT CACAGCGTGC GGACCCTTTA CCACATCAGA TACTATCACC 
TATGATGCCC TGAAGGATCT TATTGACGTT CTTAACAGAG ACTGTCCAGA TATCTGCATC 
CTGCTTGGTC CCTTTTTGGA TGCAAAGCAT GAGCAAATAG AGAACCTGCA ACTCACAGTG 
ACATTTGAAG ATGTGTTTAA GCGTTGTCTG AAAATGATTA TCGAAGGCAC ACGTCTTTCG 
GGGTGCCATC TGGTCTTCGT TCCATCTCTC AGAGATGTTC ATCATGATCC TGTCTATCCT 
CAGCCACCAT TCAGCTGTTT TGAACCGGCC AAAGAAGACA AGGACAGGGT CCATTTTGTT 
GCCGATCCCT GCACGTTGTC TGTCAATGGT GTTATCTTAG GCATAACCTC TACAGACTTG 
CTCTTTCATA TGGGTGCAGA GGAAATAAGC AGCTCTGTGG GGGCTCCGGA TCGATTTAGT 
CGGATTCTTA GACACATTCT GACCCAAAGA AGTTACTATC CACTGTATCC ACCCAATGAA 
GAAATAAATG TTGACTATGA GGCTTTCTAC AGCTACGCCC CTATGCCTGT CACTCCAGAC 
ATTTTTATAG TCCCGTCTGA GCTTCGCTAT TTTATCAAGG ATGTCACAGG CTGCATCTGC 
ATTAACCCTG GGCGGCTGAC AAAGGGACTG GTGGGCGGCA CATATGCACG CTTTCTGGTC 
AAAAGTGGAG CCATGGGGTC TGAAGGAAAG AGATCTCCTT GTATTTCTGC TCAGGTGGTG 
AGGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pola2 ( XM_012960869.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816323.1 Xenopus tropicalis polymerase (DNA directed), alpha 2, accessory subunit (pola2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa26722
Clone ID Related Accession (Same CDS sequence)	XM_012960869.2
Accession Version	XM_012960869.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA polymerase alpha subunit B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960869.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAATT TTTTTTCCTA CACGTCCATT TGGAATTGTG ATAGTTATTA TTTCTATATT 
TGCAAGTACC AGCCTGCCTC CCGCATCAAG GATCTGCCAT CCTTTGAGCA TACAACTGGT 
GAAGCCATGC CTGTGTCAGC AAAGAGCATT GAAGAGGAGC TAACGGTCTT TGACGTCAAC 
TTTGAGGATG ATGAAGTTCC AGAGAAAATG GTGGAGCTCT GTACAGTTCA CCGTCTTCAG 
GAAGAGGACA TGGTCAATGA ATGGATGGCT TTCAGTACCA CTCGGAACCT TCCGCTCACA 
GTCAGCAACC TTAATATACT GGAACATGAG GTTTTGAACA AGAAAAGTGC CAGACCACGC 
CCATCCCAGA AGAAAGAGAA ACATTGTGGA AACAGGGATT TTAATACAAT TCAAGAACTT 
ATTGAAGTGG AAACAGAAGA AGAAAATCTC CTGGATTCAT ATGCTACACC TGCTAAAGGA 
TCCCAAAAGC GTAACTTGTC TACTCCAGAA CATCCTCAGT CTAAACGCAT ATCTGTAAAC 
CGAAGCCCCC ATGTCCTCTT CTCCCCAGCA AGCTTTTCCC CCAGTGCCAC CCCGTCTCAA 
AAGTATGGCT CTCGAACCAA CCGAGGAGAA GTGGTGACAA CCTATGGAGA GTTACAAGGA 
ACTACATGGA ATGGTGGCAG TGGAAGAAAT ACTAGTGTTG AACTGTTCAC CTGTCAGGAT 
GAACCACTGA CAAAAATGTA TAAGTTCATG TTTCAGAAGT TAATGGACAT CAGAGAAGTG 
GTTAGCAGTA AGATAGAAGA ACTTGGTGCA TCTCTGAAGA ACCATTTCCA AATTGATGAG 
TTTACCTCTG TTTCTTTCCC AGCTCAAGAG ACTGTGACCG TGCTAGGACA GATTGGATGT 
GACAGCAACG GCAAGTTAAA TTCCAAATCT GTCATCCTTG AGGGGGACAG AGAGCACTCA 
GCAGGGATGC AGGTTCCAGT TGACTTGTCT GAATTAAAAG AGTATTCTCT GTTTCCAGGA 
CAGGTAGTAA TCATGGAGGG CACCAACAGC ACCGGCAGGA GGTTTGTCCC TACTAAACTT 
TATGAGGGGG TTCCACTTCC TTTTCACCAG CCCAGCAAAG AATTTGAAGA ATGTCCACAG 
CAGATGGTAA TCACAGCGTG CGGACCCTTT ACCACATCAG ATACTATCAC CTATGATGCC 
CTGAAGGATC TTATTGACGT TCTTAACAGA GACTGTCCAG ATATCTGCAT CCTGCTTGGT 
CCCTTTTTGG ATGCAAAGCA TGAGCAAATA GAGAACCTGC AACTCACAGT GACATTTGAA 
GATGTGTTTA AGCGTTGTCT GAAAATGATT ATCGAAGGCA CACGTCTTTC GGGGTGCCAT 
CTGGTCTTCG TTCCATCTCT CAGAGATGTT CATCATGATC CTGTCTATCC TCAGCCACCA 
TTCAGCTGTT TTGAACCGGC CAAAGAAGAC AAGGACAGGG TCCATTTTGT TGCCGATCCC 
TGCACGTTGT CTGTCAATGG TGTTATCTTA GGCATAACCT CTACAGACTT GCTCTTTCAT 
ATGGGTGCAG AGGAAATAAG CAGCTCTGTG GGGGCTCCGG ATCGATTTAG TCGGATTCTT 
AGACACATTC TGACCCAAAG AAGTTACTAT CCACTGTATC CACCCAATGA AGAAATAAAT 
GTTGACTATG AGGCTTTCTA CAGCTACGCC CCTATGCCTG TCACTCCAGA CATTTTTATA 
GTCCCGTCTG AGCTTCGCTA TTTTATCAAG GATGTCACAG GCTGCATCTG CATTAACCCT 
GGGCGGCTGA CAAAGGGACT GGTGGGCGGC ACATATGCAC GCTTTCTGGT CAAAAGTGGA 
GCCATGGGGT CTGAAGGAAA GAGATCTCCT TGTATTTCTG CTCAGGTGGT GAGGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816323.1
CDS	214..2133
Translation	MRNFFSYTSIWNCDSYYFYICKYQPASRIKDLPSFEHTTGEAMPVSAKSIEEELTVFDVNFEDDEVPEKMVELCTVHRLQEEDMVNEWMAFSTTRNLPLTVSNLNILEHEVLNKKSARPRPSQKKEKHCGNRDFNTIQELIEVETEEENLLDSYATPAKGSQKRNLSTPEHPQSKRISVNRSPHVLFSPASFSPSATPSQKYGSRTNRGEVVTTYGELQGTTWNGGSGRNTSVELFTCQDEPLTKMYKFMFQKLMDIREVVSSKIEELGASLKNHFQIDEFTSVSFPAQETVTVLGQIGCDSNGKLNSKSVILEGDREHSAGMQVPVDLSELKEYSLFPGQVVIMEGTNSTGRRFVPTKLYEGVPLPFHQPSKEFEECPQQMVITACGPFTTSDTITYDALKDLIDVLNRDCPDICILLGPFLDAKHEQIENLQLTVTFEDVFKRCLKMIIEGTRLSGCHLVFVPSLRDVHHDPVYPQPPFSCFEPAKEDKDRVHFVADPCTLSVNGVILGITSTDLLFHMGAEEISSSVGAPDRFSRILRHILTQRSYYPLYPPNEEINVDYEAFYSYAPMPVTPDIFIVPSELRYFIKDVTGCICINPGRLTKGLVGGTYARFLVKSGAMGSEGKRSPCISAQVVRI

Target ORF information:

RefSeq Version	XM_012960869.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis polymerase (DNA directed), alpha 2, accessory subunit (pola2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960869.2

ORF Insert Sequence:

ATGCGCAATT TTTTTTCCTA CACGTCCATT TGGAATTGTG ATAGTTATTA TTTCTATATT 
TGCAAGTACC AGCCTGCCTC CCGCATCAAG GATCTGCCAT CCTTTGAGCA TACAACTGGT 
GAAGCCATGC CTGTGTCAGC AAAGAGCATT GAAGAGGAGC TAACGGTCTT TGACGTCAAC 
TTTGAGGATG ATGAAGTTCC AGAGAAAATG GTGGAGCTCT GTACAGTTCA CCGTCTTCAG 
GAAGAGGACA TGGTCAATGA ATGGATGGCT TTCAGTACCA CTCGGAACCT TCCGCTCACA 
GTCAGCAACC TTAATATACT GGAACATGAG GTTTTGAACA AGAAAAGTGC CAGACCACGC 
CCATCCCAGA AGAAAGAGAA ACATTGTGGA AACAGGGATT TTAATACAAT TCAAGAACTT 
ATTGAAGTGG AAACAGAAGA AGAAAATCTC CTGGATTCAT ATGCTACACC TGCTAAAGGA 
TCCCAAAAGC GTAACTTGTC TACTCCAGAA CATCCTCAGT CTAAACGCAT ATCTGTAAAC 
CGAAGCCCCC ATGTCCTCTT CTCCCCAGCA AGCTTTTCCC CCAGTGCCAC CCCGTCTCAA 
AAGTATGGCT CTCGAACCAA CCGAGGAGAA GTGGTGACAA CCTATGGAGA GTTACAAGGA 
ACTACATGGA ATGGTGGCAG TGGAAGAAAT ACTAGTGTTG AACTGTTCAC CTGTCAGGAT 
GAACCACTGA CAAAAATGTA TAAGTTCATG TTTCAGAAGT TAATGGACAT CAGAGAAGTG 
GTTAGCAGTA AGATAGAAGA ACTTGGTGCA TCTCTGAAGA ACCATTTCCA AATTGATGAG 
TTTACCTCTG TTTCTTTCCC AGCTCAAGAG ACTGTGACCG TGCTAGGACA GATTGGATGT 
GACAGCAACG GCAAGTTAAA TTCCAAATCT GTCATCCTTG AGGGGGACAG AGAGCACTCA 
GCAGGGATGC AGGTTCCAGT TGACTTGTCT GAATTAAAAG AGTATTCTCT GTTTCCAGGA 
CAGGTAGTAA TCATGGAGGG CACCAACAGC ACCGGCAGGA GGTTTGTCCC TACTAAACTT 
TATGAGGGGG TTCCACTTCC TTTTCACCAG CCCAGCAAAG AATTTGAAGA ATGTCCACAG 
CAGATGGTAA TCACAGCGTG CGGACCCTTT ACCACATCAG ATACTATCAC CTATGATGCC 
CTGAAGGATC TTATTGACGT TCTTAACAGA GACTGTCCAG ATATCTGCAT CCTGCTTGGT 
CCCTTTTTGG ATGCAAAGCA TGAGCAAATA GAGAACCTGC AACTCACAGT GACATTTGAA 
GATGTGTTTA AGCGTTGTCT GAAAATGATT ATCGAAGGCA CACGTCTTTC GGGGTGCCAT 
CTGGTCTTCG TTCCATCTCT CAGAGATGTT CATCATGATC CTGTCTATCC TCAGCCACCA 
TTCAGCTGTT TTGAACCGGC CAAAGAAGAC AAGGACAGGG TCCATTTTGT TGCCGATCCC 
TGCACGTTGT CTGTCAATGG TGTTATCTTA GGCATAACCT CTACAGACTT GCTCTTTCAT 
ATGGGTGCAG AGGAAATAAG CAGCTCTGTG GGGGCTCCGG ATCGATTTAG TCGGATTCTT 
AGACACATTC TGACCCAAAG AAGTTACTAT CCACTGTATC CACCCAATGA AGAAATAAAT 
GTTGACTATG AGGCTTTCTA CAGCTACGCC CCTATGCCTG TCACTCCAGA CATTTTTATA 
GTCCCGTCTG AGCTTCGCTA TTTTATCAAG GATGTCACAG GCTGCATCTG CATTAACCCT 
GGGCGGCTGA CAAAGGGACT GGTGGGCGGC ACATATGCAC GCTTTCTGGT CAAAAGTGGA 
GCCATGGGGT CTGAAGGAAA GAGATCTCCT TGTATTTCTG CTCAGGTGGT GAGGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pola2 ( XM_012960870.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816324.1 Xenopus tropicalis polymerase (DNA directed), alpha 2, accessory subunit (pola2), transcript variant X1, mRNA.

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CloneID	OXa20631
Clone ID Related Accession (Same CDS sequence)	NM_001005060.1 , XM_012960870.3 , XM_012960871.3 , XM_012960870.2 , XM_012960871.2
Accession Version	XM_012960870.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA polymerase alpha subunit B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960870.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGT CAGCAAAGAG CATTGAAGAG GAGCTAACGG TCTTTGACGT CAACTTTGAG 
GATGATGAAG TTCCAGAGAA AATGGTGGAG CTCTGTACAG TTCACCGTCT TCAGGAAGAG 
GACATGGTCA ATGAATGGAT GGCTTTCAGT ACCACTCGGA ACCTTCCGCT CACAGTCAGC 
AACCTTAATA TACTGGAACA TGAGGTTTTG AACAAGAAAA GTGCCAGACC ACGCCCATCC 
CAGAAGAAAG AGAAACATTG TGGAAACAGG GATTTTAATA CAATTCAAGA ACTTATTGAA 
GTGGAAACAG AAGAAGAAAA TCTCCTGGAT TCATATGCTA CACCTGCTAA AGGATCCCAA 
AAGCGTAACT TGTCTACTCC AGAACATCCT CAGTCTAAAC GCATATCTGT AAACCGAAGC 
CCCCATGTCC TCTTCTCCCC AGCAAGCTTT TCCCCCAGTG CCACCCCGTC TCAAAAGTAT 
GGCTCTCGAA CCAACCGAGG AGAAGTGGTG ACAACCTATG GAGAGTTACA AGGAACTACA 
TGGAATGGTG GCAGTGGAAG AAATACTAGT GTTGAACTGT TCACCTGTCA GGATGAACCA 
CTGACAAAAA TGTATAAGTT CATGTTTCAG AAGTTAATGG ACATCAGAGA AGTGGTTAGC 
AGTAAGATAG AAGAACTTGG TGCATCTCTG AAGAACCATT TCCAAATTGA TGAGTTTACC 
TCTGTTTCTT TCCCAGCTCA AGAGACTGTG ACCGTGCTAG GACAGATTGG ATGTGACAGC 
AACGGCAAGT TAAATTCCAA ATCTGTCATC CTTGAGGGGG ACAGAGAGCA CTCAGCAGGG 
ATGCAGGTTC CAGTTGACTT GTCTGAATTA AAAGAGTATT CTCTGTTTCC AGGACAGGTA 
GTAATCATGG AGGGCACCAA CAGCACCGGC AGGAGGTTTG TCCCTACTAA ACTTTATGAG 
GGGGTTCCAC TTCCTTTTCA CCAGCCCAGC AAAGAATTTG AAGAATGTCC ACAGCAGATG 
GTAATCACAG CGTGCGGACC CTTTACCACA TCAGATACTA TCACCTATGA TGCCCTGAAG 
GATCTTATTG ACGTTCTTAA CAGAGACTGT CCAGATATCT GCATCCTGCT TGGTCCCTTT 
TTGGATGCAA AGCATGAGCA AATAGAGAAC CTGCAACTCA CAGTGACATT TGAAGATGTG 
TTTAAGCGTT GTCTGAAAAT GATTATCGAA GGCACACGTC TTTCGGGGTG CCATCTGGTC 
TTCGTTCCAT CTCTCAGAGA TGTTCATCAT GATCCTGTCT ATCCTCAGCC ACCATTCAGC 
TGTTTTGAAC CGGCCAAAGA AGACAAGGAC AGGGTCCATT TTGTTGCCGA TCCCTGCACG 
TTGTCTGTCA ATGGTGTTAT CTTAGGCATA ACCTCTACAG ACTTGCTCTT TCATATGGGT 
GCAGAGGAAA TAAGCAGCTC TGTGGGGGCT CCGGATCGAT TTAGTCGGAT TCTTAGACAC 
ATTCTGACCC AAAGAAGTTA CTATCCACTG TATCCACCCA ATGAAGAAAT AAATGTTGAC 
TATGAGGCTT TCTACAGCTA CGCCCCTATG CCTGTCACTC CAGACATTTT TATAGTCCCG 
TCTGAGCTTC GCTATTTTAT CAAGGATGTC ACAGGCTGCA TCTGCATTAA CCCTGGGCGG 
CTGACAAAGG GACTGGTGGG CGGCACATAT GCACGCTTTC TGGTCAAAAG TGGAGCCATG 
GGGTCTGAAG GAAAGAGATC TCCTTGTATT TCTGCTCAGG TGGTGAGGAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816324.1
CDS	253..2046
Translation	MPVSAKSIEEELTVFDVNFEDDEVPEKMVELCTVHRLQEEDMVNEWMAFSTTRNLPLTVSNLNILEHEVLNKKSARPRPSQKKEKHCGNRDFNTIQELIEVETEEENLLDSYATPAKGSQKRNLSTPEHPQSKRISVNRSPHVLFSPASFSPSATPSQKYGSRTNRGEVVTTYGELQGTTWNGGSGRNTSVELFTCQDEPLTKMYKFMFQKLMDIREVVSSKIEELGASLKNHFQIDEFTSVSFPAQETVTVLGQIGCDSNGKLNSKSVILEGDREHSAGMQVPVDLSELKEYSLFPGQVVIMEGTNSTGRRFVPTKLYEGVPLPFHQPSKEFEECPQQMVITACGPFTTSDTITYDALKDLIDVLNRDCPDICILLGPFLDAKHEQIENLQLTVTFEDVFKRCLKMIIEGTRLSGCHLVFVPSLRDVHHDPVYPQPPFSCFEPAKEDKDRVHFVADPCTLSVNGVILGITSTDLLFHMGAEEISSSVGAPDRFSRILRHILTQRSYYPLYPPNEEINVDYEAFYSYAPMPVTPDIFIVPSELRYFIKDVTGCICINPGRLTKGLVGGTYARFLVKSGAMGSEGKRSPCISAQVVRI

Target ORF information:

RefSeq Version	XM_012960870.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis polymerase (DNA directed), alpha 2, accessory subunit (pola2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960870.3

ORF Insert Sequence:

ATGCCTGTGT CAGCAAAGAG CATTGAAGAG GAGCTAACGG TCTTTGACGT CAACTTTGAG 
GATGATGAAG TTCCAGAGAA AATGGTGGAG CTCTGTACAG TTCACCGTCT TCAGGAAGAG 
GACATGGTCA ATGAATGGAT GGCTTTCAGT ACCACTCGGA ACCTTCCGCT CACAGTCAGC 
AACCTTAATA TACTGGAACA TGAGGTTTTG AACAAGAAAA GTGCCAGACC ACGCCCATCC 
CAGAAGAAAG AGAAACATTG TGGAAACAGG GATTTTAATA CAATTCAAGA ACTTATTGAA 
GTGGAAACAG AAGAAGAAAA TCTCCTGGAT TCATATGCTA CACCTGCTAA AGGATCCCAA 
AAGCGTAACT TGTCTACTCC AGAACATCCT CAGTCTAAAC GCATATCTGT AAACCGAAGC 
CCCCATGTCC TCTTCTCCCC AGCAAGCTTT TCCCCCAGTG CCACCCCGTC TCAAAAGTAT 
GGCTCTCGAA CCAACCGAGG AGAAGTGGTG ACAACCTATG GAGAGTTACA AGGAACTACA 
TGGAATGGTG GCAGTGGAAG AAATACTAGT GTTGAACTGT TCACCTGTCA GGATGAACCA 
CTGACAAAAA TGTATAAGTT CATGTTTCAG AAGTTAATGG ACATCAGAGA AGTGGTTAGC 
AGTAAGATAG AAGAACTTGG TGCATCTCTG AAGAACCATT TCCAAATTGA TGAGTTTACC 
TCTGTTTCTT TCCCAGCTCA AGAGACTGTG ACCGTGCTAG GACAGATTGG ATGTGACAGC 
AACGGCAAGT TAAATTCCAA ATCTGTCATC CTTGAGGGGG ACAGAGAGCA CTCAGCAGGG 
ATGCAGGTTC CAGTTGACTT GTCTGAATTA AAAGAGTATT CTCTGTTTCC AGGACAGGTA 
GTAATCATGG AGGGCACCAA CAGCACCGGC AGGAGGTTTG TCCCTACTAA ACTTTATGAG 
GGGGTTCCAC TTCCTTTTCA CCAGCCCAGC AAAGAATTTG AAGAATGTCC ACAGCAGATG 
GTAATCACAG CGTGCGGACC CTTTACCACA TCAGATACTA TCACCTATGA TGCCCTGAAG 
GATCTTATTG ACGTTCTTAA CAGAGACTGT CCAGATATCT GCATCCTGCT TGGTCCCTTT 
TTGGATGCAA AGCATGAGCA AATAGAGAAC CTGCAACTCA CAGTGACATT TGAAGATGTG 
TTTAAGCGTT GTCTGAAAAT GATTATCGAA GGCACACGTC TTTCGGGGTG CCATCTGGTC 
TTCGTTCCAT CTCTCAGAGA TGTTCATCAT GATCCTGTCT ATCCTCAGCC ACCATTCAGC 
TGTTTTGAAC CGGCCAAAGA AGACAAGGAC AGGGTCCATT TTGTTGCCGA TCCCTGCACG 
TTGTCTGTCA ATGGTGTTAT CTTAGGCATA ACCTCTACAG ACTTGCTCTT TCATATGGGT 
GCAGAGGAAA TAAGCAGCTC TGTGGGGGCT CCGGATCGAT TTAGTCGGAT TCTTAGACAC 
ATTCTGACCC AAAGAAGTTA CTATCCACTG TATCCACCCA ATGAAGAAAT AAATGTTGAC 
TATGAGGCTT TCTACAGCTA CGCCCCTATG CCTGTCACTC CAGACATTTT TATAGTCCCG 
TCTGAGCTTC GCTATTTTAT CAAGGATGTC ACAGGCTGCA TCTGCATTAA CCCTGGGCGG 
CTGACAAAGG GACTGGTGGG CGGCACATAT GCACGCTTTC TGGTCAAAAG TGGAGCCATG 
GGGTCTGAAG GAAAGAGATC TCCTTGTATT TCTGCTCAGG TGGTGAGGAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pola2 ( XM_012960871.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816325.1 Xenopus tropicalis polymerase (DNA directed), alpha 2, accessory subunit (pola2), transcript variant X2, mRNA.

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CloneID	OXa20631
Clone ID Related Accession (Same CDS sequence)	NM_001005060.1 , XM_012960870.3 , XM_012960871.3 , XM_012960870.2 , XM_012960871.2
Accession Version	XM_012960871.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA polymerase alpha subunit B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960871.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGT CAGCAAAGAG CATTGAAGAG GAGCTAACGG TCTTTGACGT CAACTTTGAG 
GATGATGAAG TTCCAGAGAA AATGGTGGAG CTCTGTACAG TTCACCGTCT TCAGGAAGAG 
GACATGGTCA ATGAATGGAT GGCTTTCAGT ACCACTCGGA ACCTTCCGCT CACAGTCAGC 
AACCTTAATA TACTGGAACA TGAGGTTTTG AACAAGAAAA GTGCCAGACC ACGCCCATCC 
CAGAAGAAAG AGAAACATTG TGGAAACAGG GATTTTAATA CAATTCAAGA ACTTATTGAA 
GTGGAAACAG AAGAAGAAAA TCTCCTGGAT TCATATGCTA CACCTGCTAA AGGATCCCAA 
AAGCGTAACT TGTCTACTCC AGAACATCCT CAGTCTAAAC GCATATCTGT AAACCGAAGC 
CCCCATGTCC TCTTCTCCCC AGCAAGCTTT TCCCCCAGTG CCACCCCGTC TCAAAAGTAT 
GGCTCTCGAA CCAACCGAGG AGAAGTGGTG ACAACCTATG GAGAGTTACA AGGAACTACA 
TGGAATGGTG GCAGTGGAAG AAATACTAGT GTTGAACTGT TCACCTGTCA GGATGAACCA 
CTGACAAAAA TGTATAAGTT CATGTTTCAG AAGTTAATGG ACATCAGAGA AGTGGTTAGC 
AGTAAGATAG AAGAACTTGG TGCATCTCTG AAGAACCATT TCCAAATTGA TGAGTTTACC 
TCTGTTTCTT TCCCAGCTCA AGAGACTGTG ACCGTGCTAG GACAGATTGG ATGTGACAGC 
AACGGCAAGT TAAATTCCAA ATCTGTCATC CTTGAGGGGG ACAGAGAGCA CTCAGCAGGG 
ATGCAGGTTC CAGTTGACTT GTCTGAATTA AAAGAGTATT CTCTGTTTCC AGGACAGGTA 
GTAATCATGG AGGGCACCAA CAGCACCGGC AGGAGGTTTG TCCCTACTAA ACTTTATGAG 
GGGGTTCCAC TTCCTTTTCA CCAGCCCAGC AAAGAATTTG AAGAATGTCC ACAGCAGATG 
GTAATCACAG CGTGCGGACC CTTTACCACA TCAGATACTA TCACCTATGA TGCCCTGAAG 
GATCTTATTG ACGTTCTTAA CAGAGACTGT CCAGATATCT GCATCCTGCT TGGTCCCTTT 
TTGGATGCAA AGCATGAGCA AATAGAGAAC CTGCAACTCA CAGTGACATT TGAAGATGTG 
TTTAAGCGTT GTCTGAAAAT GATTATCGAA GGCACACGTC TTTCGGGGTG CCATCTGGTC 
TTCGTTCCAT CTCTCAGAGA TGTTCATCAT GATCCTGTCT ATCCTCAGCC ACCATTCAGC 
TGTTTTGAAC CGGCCAAAGA AGACAAGGAC AGGGTCCATT TTGTTGCCGA TCCCTGCACG 
TTGTCTGTCA ATGGTGTTAT CTTAGGCATA ACCTCTACAG ACTTGCTCTT TCATATGGGT 
GCAGAGGAAA TAAGCAGCTC TGTGGGGGCT CCGGATCGAT TTAGTCGGAT TCTTAGACAC 
ATTCTGACCC AAAGAAGTTA CTATCCACTG TATCCACCCA ATGAAGAAAT AAATGTTGAC 
TATGAGGCTT TCTACAGCTA CGCCCCTATG CCTGTCACTC CAGACATTTT TATAGTCCCG 
TCTGAGCTTC GCTATTTTAT CAAGGATGTC ACAGGCTGCA TCTGCATTAA CCCTGGGCGG 
CTGACAAAGG GACTGGTGGG CGGCACATAT GCACGCTTTC TGGTCAAAAG TGGAGCCATG 
GGGTCTGAAG GAAAGAGATC TCCTTGTATT TCTGCTCAGG TGGTGAGGAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816325.1
CDS	94..1887
Translation	MPVSAKSIEEELTVFDVNFEDDEVPEKMVELCTVHRLQEEDMVNEWMAFSTTRNLPLTVSNLNILEHEVLNKKSARPRPSQKKEKHCGNRDFNTIQELIEVETEEENLLDSYATPAKGSQKRNLSTPEHPQSKRISVNRSPHVLFSPASFSPSATPSQKYGSRTNRGEVVTTYGELQGTTWNGGSGRNTSVELFTCQDEPLTKMYKFMFQKLMDIREVVSSKIEELGASLKNHFQIDEFTSVSFPAQETVTVLGQIGCDSNGKLNSKSVILEGDREHSAGMQVPVDLSELKEYSLFPGQVVIMEGTNSTGRRFVPTKLYEGVPLPFHQPSKEFEECPQQMVITACGPFTTSDTITYDALKDLIDVLNRDCPDICILLGPFLDAKHEQIENLQLTVTFEDVFKRCLKMIIEGTRLSGCHLVFVPSLRDVHHDPVYPQPPFSCFEPAKEDKDRVHFVADPCTLSVNGVILGITSTDLLFHMGAEEISSSVGAPDRFSRILRHILTQRSYYPLYPPNEEINVDYEAFYSYAPMPVTPDIFIVPSELRYFIKDVTGCICINPGRLTKGLVGGTYARFLVKSGAMGSEGKRSPCISAQVVRI

Target ORF information:

RefSeq Version	XM_012960871.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis polymerase (DNA directed), alpha 2, accessory subunit (pola2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960871.3

ORF Insert Sequence:

ATGCCTGTGT CAGCAAAGAG CATTGAAGAG GAGCTAACGG TCTTTGACGT CAACTTTGAG 
GATGATGAAG TTCCAGAGAA AATGGTGGAG CTCTGTACAG TTCACCGTCT TCAGGAAGAG 
GACATGGTCA ATGAATGGAT GGCTTTCAGT ACCACTCGGA ACCTTCCGCT CACAGTCAGC 
AACCTTAATA TACTGGAACA TGAGGTTTTG AACAAGAAAA GTGCCAGACC ACGCCCATCC 
CAGAAGAAAG AGAAACATTG TGGAAACAGG GATTTTAATA CAATTCAAGA ACTTATTGAA 
GTGGAAACAG AAGAAGAAAA TCTCCTGGAT TCATATGCTA CACCTGCTAA AGGATCCCAA 
AAGCGTAACT TGTCTACTCC AGAACATCCT CAGTCTAAAC GCATATCTGT AAACCGAAGC 
CCCCATGTCC TCTTCTCCCC AGCAAGCTTT TCCCCCAGTG CCACCCCGTC TCAAAAGTAT 
GGCTCTCGAA CCAACCGAGG AGAAGTGGTG ACAACCTATG GAGAGTTACA AGGAACTACA 
TGGAATGGTG GCAGTGGAAG AAATACTAGT GTTGAACTGT TCACCTGTCA GGATGAACCA 
CTGACAAAAA TGTATAAGTT CATGTTTCAG AAGTTAATGG ACATCAGAGA AGTGGTTAGC 
AGTAAGATAG AAGAACTTGG TGCATCTCTG AAGAACCATT TCCAAATTGA TGAGTTTACC 
TCTGTTTCTT TCCCAGCTCA AGAGACTGTG ACCGTGCTAG GACAGATTGG ATGTGACAGC 
AACGGCAAGT TAAATTCCAA ATCTGTCATC CTTGAGGGGG ACAGAGAGCA CTCAGCAGGG 
ATGCAGGTTC CAGTTGACTT GTCTGAATTA AAAGAGTATT CTCTGTTTCC AGGACAGGTA 
GTAATCATGG AGGGCACCAA CAGCACCGGC AGGAGGTTTG TCCCTACTAA ACTTTATGAG 
GGGGTTCCAC TTCCTTTTCA CCAGCCCAGC AAAGAATTTG AAGAATGTCC ACAGCAGATG 
GTAATCACAG CGTGCGGACC CTTTACCACA TCAGATACTA TCACCTATGA TGCCCTGAAG 
GATCTTATTG ACGTTCTTAA CAGAGACTGT CCAGATATCT GCATCCTGCT TGGTCCCTTT 
TTGGATGCAA AGCATGAGCA AATAGAGAAC CTGCAACTCA CAGTGACATT TGAAGATGTG 
TTTAAGCGTT GTCTGAAAAT GATTATCGAA GGCACACGTC TTTCGGGGTG CCATCTGGTC 
TTCGTTCCAT CTCTCAGAGA TGTTCATCAT GATCCTGTCT ATCCTCAGCC ACCATTCAGC 
TGTTTTGAAC CGGCCAAAGA AGACAAGGAC AGGGTCCATT TTGTTGCCGA TCCCTGCACG 
TTGTCTGTCA ATGGTGTTAT CTTAGGCATA ACCTCTACAG ACTTGCTCTT TCATATGGGT 
GCAGAGGAAA TAAGCAGCTC TGTGGGGGCT CCGGATCGAT TTAGTCGGAT TCTTAGACAC 
ATTCTGACCC AAAGAAGTTA CTATCCACTG TATCCACCCA ATGAAGAAAT AAATGTTGAC 
TATGAGGCTT TCTACAGCTA CGCCCCTATG CCTGTCACTC CAGACATTTT TATAGTCCCG 
TCTGAGCTTC GCTATTTTAT CAAGGATGTC ACAGGCTGCA TCTGCATTAA CCCTGGGCGG 
CTGACAAAGG GACTGGTGGG CGGCACATAT GCACGCTTTC TGGTCAAAAG TGGAGCCATG 
GGGTCTGAAG GAAAGAGATC TCCTTGTATT TCTGCTCAGG TGGTGAGGAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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pola2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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