eif2d cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	eif2d
Entrez Gene ID	395035
Full Name	eukaryotic translation initiation factor 2D
Synonyms	lgtn
General protein information	Preferred Names eukaryotic translation initiation factor 2D Names eukaryotic translation initiation factor 2D ligatin
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012958645.3	XP_012814099.1	eukaryotic translation initiation factor 2D isoform X1
XM_012958655.2	XP_012814109.1	eukaryotic translation initiation factor 2D isoform X3
XM_018091152.1	XP_017946641.1	eukaryotic translation initiation factor 2D isoform X4
XM_012958652.3	XP_012814106.1	eukaryotic translation initiation factor 2D isoform X2
XM_012958645.2	XP_012814099.1	eukaryotic translation initiation factor 2D isoform X1
XM_012958652.2	XP_012814106.1	eukaryotic translation initiation factor 2D isoform X2
NM_204067.1	NP_989398.1	eukaryotic translation initiation factor 2D

Pathways from BioSystems

Homologies

	EIF2D	XP_001086830.1
Gallus gallus (chicken)	EIF2D	NP_001006322.2
Xenopus tropicalis (tropical clawed frog)	eif2d	NP_989398.1
Canis lupus familiaris (dog)	EIF2D	NP_001189417.1
Bos taurus (cattle)	EIF2D	NP_001030276.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011804	XP_320711.4
Arabidopsis thaliana (thale cress)	AT1G71350	NP_177291.2
Homo sapiens (human)	EIF2D	NP_008824.2
Mus musculus (house mouse)	Eif2d	NP_001129542.1
Drosophila melanogaster (fruit fly)	ligatin	NP_477481.1
Oryza sativa (rice)	Os02g0557600	NP_001047140.1
Pan troglodytes (chimpanzee)	EIF2D	NP_001189416.1
Rattus norvegicus (Norway rat)	Eif2d	NP_001017489.1
Danio rerio (zebrafish)	eif2d	NP_957456.1
Caenorhabditis elegans (roundworm)	CELE_C25H3.4	NP_495114.3

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following eif2d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eif2d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa25204	NM_204067.1 Latest version!	Xenopus tropicalis eukaryotic translation initiation factor 2D (eif2d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa26192	XM_012958655.2 Latest version!	Xenopus tropicalis eukaryotic translation initiation factor 2D (eif2d), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa42493	XM_018091152.1 Latest version!	Xenopus tropicalis eukaryotic translation initiation factor 2D (eif2d), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OXa26190	XM_012958645.3 Latest version!	Xenopus tropicalis eukaryotic translation initiation factor 2D (eif2d), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa26191	XM_012958652.3 Latest version!	Xenopus tropicalis eukaryotic translation initiation factor 2D (eif2d), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OXa26190	XM_012958645.2	Xenopus tropicalis eukaryotic translation initiation factor 2D (eif2d), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa26191	XM_012958652.2	Xenopus tropicalis eukaryotic translation initiation factor 2D (eif2d), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eif2d ( NM_204067.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_989398.1 Xenopus tropicalis eukaryotic translation initiation factor 2D (eif2d), mRNA.

Price & Availability↑

CloneID	OXa25204
Clone ID Related Accession (Same CDS sequence)	NM_204067.1
Accession Version	NM_204067.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 2D
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC064862.1. ##Evidence-Data-START## Transcript exon combination :: BC064862.1, CR760131.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGTTTGCTA AGAATTTTAG GGTTAAGTCA AATACAGCTA TAAAGGGATC GGACAGGAGG 
AAGCTGCGAG CAGATATCAG TTCTGCATTT CCTTTTCTTA CCACAGAAAA CTTGTCTGAG 
TTGATGCCAT CTAAAGAGGA ACTGAGTATT GTAAAAGTCT TTGCTCACAA AGGAGATGCA 
GTGACATTAT ACGTCCACAA TCGAAACCCA GTTATGTTTG AACTGGAGAA AAACCTTTAT 
CCAACAGTAT ATGCTTTGTG GAAGTATCCA GATCTTCTCC CAGCATTCAC CACATGGCCA 
CCTGTCCTGG AGAAAATGGC AGGTGGAGCA GATCTGATGC TGCCTGGTGT TGTTGTCCCC 
TCATTTGGTC TTCCTGAGAT ACAACAAGGC AGCCTATGTG CAATCACCTT GGTGGGAAAC 
AGGGCACCTG TAGCGGTTGG TGTGGCCACT ATGTCATCGA AAGAGATGCT GGCATCAGGA 
ATGAAAGGGA AAGGTTTCAA TATTTTGCAC ACATTTGGAG ATCAGTTATG GGCATATGGA 
GACAAGTCTT GTTTGCCAAA TATCCCCATA CCGGAGGTCA GTCTAACAGT AGTGGAAGAA 
GGAGATGGGC AAGCTGTGTC CCAGGAGGTC TCCTCCGAAG CCCAGGATCC ACAAGCACAG 
CTGGAGAATC TCAATCTCAT GGAGGAAGAA ACCACACACA AAGAAGAGGA TTCTGAAAAA 
CATTGTGAGG GTGTAGATGA ACAGATTAAA GAGACAGAAA ATTTAGTTCC TTTACAGAAG 
TCGTCTCAAG AAGCTATGGA CAGTTTGCTG CACCAGTGCT TCTTTCATGC ATTGAAGCAT 
AAAGTGAAAA AATCCGATCT TCCTCTTCTT ACAAGCACAT TTCTGCGCAA TTATCTTTTT 
GCCTGCTGTC CAGAAGGACA GCAAGTGGAT ATTAAAAAAT CAAGTTACAA AAAGCTAACT 
AAGTTTTTAC AAACTATGCA ACAGCGAAAG GTTTTGCAGG TGAAGGAGCT CAATAAGGGA 
GTTGAGAGCA TAGTGGAAAT AGACTGGAAG CATCCTGATG TTCGTTCCTT TGTTGCACCA 
GAGCCTGTAC CAGTGGACTT GGTAGTTTCT GGTAGCATAG ATGGGGGTGG AGAATCGCCA 
TACCAGCCCC CTGAAATAAT AACCCTCTAT GGTATTTCCA GTAAAATGCT GCCTCTTTTC 
CAAGAGGCAG GGCATAGAAA AGGAGATCTG CTCACCCATA GTGATGTTAG AAGCATCATT 
ATTACTTATG TGAAAAACAA TGAGCTTGTT GATTCAAATA ATAAGAACTT TGTGAATATA 
AACCCAGTTC TGTGTGATTG CCTGCTTGAA AAATCAGAGC AGAATGACTT TTTCAGTCTG 
AAATGGGATG ACCTTCTCTT AAGGTGTCTA GAGAAGATGC AGCATTCCCA CCAAGTGACC 
TTCCCTGGAC GACCACCTAT TATACGAAAA GGAAACATTG ATCCCATTGA TATCAGTGTG 
GTGCAGAGGG CGTCAAACAA AAAGGTGACA ATGATTAAAA ATCTTGAATT GTATGGACTA 
GATCCCTCTT CAGTTGGGAG CATGCTCCAA CAGCGTGTTC AAGCCAGTTC CACATGCACT 
GCCATTCCAG GGTCTAAGGA CAGAGTGCAG GTGCAGATAC AGGGCAACCA GGTGTCTCAG 
GTTGGAAAAC TACTACTTGA AGAGTTTAAA ATACCCCGCA AATACATTTC TGGATTGGAC 
CTGGCTAAAT CTGGTAAAAA GAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989398.1
CDS	86..1852
Translation	MFAKNFRVKSNTAIKGSDRRKLRADISSAFPFLTTENLSELMPSKEELSIVKVFAHKGDAVTLYVHNRNPVMFELEKNLYPTVYALWKYPDLLPAFTTWPPVLEKMAGGADLMLPGVVVPSFGLPEIQQGSLCAITLVGNRAPVAVGVATMSSKEMLASGMKGKGFNILHTFGDQLWAYGDKSCLPNIPIPEVSLTVVEEGDGQAVSQEVSSEAQDPQAQLENLNLMEEETTHKEEDSEKHCEGVDEQIKETENLVPLQKSSQEAMDSLLHQCFFHALKHKVKKSDLPLLTSTFLRNYLFACCPEGQQVDIKKSSYKKLTKFLQTMQQRKVLQVKELNKGVESIVEIDWKHPDVRSFVAPEPVPVDLVVSGSIDGGGESPYQPPEIITLYGISSKMLPLFQEAGHRKGDLLTHSDVRSIIITYVKNNELVDSNNKNFVNINPVLCDCLLEKSEQNDFFSLKWDDLLLRCLEKMQHSHQVTFPGRPPIIRKGNIDPIDISVVQRASNKKVTMIKNLELYGLDPSSVGSMLQQRVQASSTCTAIPGSKDRVQVQIQGNQVSQVGKLLLEEFKIPRKYISGLDLAKSGKKK

Target ORF information:

RefSeq Version	NM_204067.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 2D (eif2d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204067.1

ORF Insert Sequence:

ATGTTTGCTA AGAATTTTAG GGTTAAGTCA AATACAGCTA TAAAGGGATC GGACAGGAGG 
AAGCTGCGAG CAGATATCAG TTCTGCATTT CCTTTTCTTA CCACAGAAAA CTTGTCTGAG 
TTGATGCCAT CTAAAGAGGA ACTGAGTATT GTAAAAGTCT TTGCTCACAA AGGAGATGCA 
GTGACATTAT ACGTCCACAA TCGAAACCCA GTTATGTTTG AACTGGAGAA AAACCTTTAT 
CCAACAGTAT ATGCTTTGTG GAAGTATCCA GATCTTCTCC CAGCATTCAC CACATGGCCA 
CCTGTCCTGG AGAAAATGGC AGGTGGAGCA GATCTGATGC TGCCTGGTGT TGTTGTCCCC 
TCATTTGGTC TTCCTGAGAT ACAACAAGGC AGCCTATGTG CAATCACCTT GGTGGGAAAC 
AGGGCACCTG TAGCGGTTGG TGTGGCCACT ATGTCATCGA AAGAGATGCT GGCATCAGGA 
ATGAAAGGGA AAGGTTTCAA TATTTTGCAC ACATTTGGAG ATCAGTTATG GGCATATGGA 
GACAAGTCTT GTTTGCCAAA TATCCCCATA CCGGAGGTCA GTCTAACAGT AGTGGAAGAA 
GGAGATGGGC AAGCTGTGTC CCAGGAGGTC TCCTCCGAAG CCCAGGATCC ACAAGCACAG 
CTGGAGAATC TCAATCTCAT GGAGGAAGAA ACCACACACA AAGAAGAGGA TTCTGAAAAA 
CATTGTGAGG GTGTAGATGA ACAGATTAAA GAGACAGAAA ATTTAGTTCC TTTACAGAAG 
TCGTCTCAAG AAGCTATGGA CAGTTTGCTG CACCAGTGCT TCTTTCATGC ATTGAAGCAT 
AAAGTGAAAA AATCCGATCT TCCTCTTCTT ACAAGCACAT TTCTGCGCAA TTATCTTTTT 
GCCTGCTGTC CAGAAGGACA GCAAGTGGAT ATTAAAAAAT CAAGTTACAA AAAGCTAACT 
AAGTTTTTAC AAACTATGCA ACAGCGAAAG GTTTTGCAGG TGAAGGAGCT CAATAAGGGA 
GTTGAGAGCA TAGTGGAAAT AGACTGGAAG CATCCTGATG TTCGTTCCTT TGTTGCACCA 
GAGCCTGTAC CAGTGGACTT GGTAGTTTCT GGTAGCATAG ATGGGGGTGG AGAATCGCCA 
TACCAGCCCC CTGAAATAAT AACCCTCTAT GGTATTTCCA GTAAAATGCT GCCTCTTTTC 
CAAGAGGCAG GGCATAGAAA AGGAGATCTG CTCACCCATA GTGATGTTAG AAGCATCATT 
ATTACTTATG TGAAAAACAA TGAGCTTGTT GATTCAAATA ATAAGAACTT TGTGAATATA 
AACCCAGTTC TGTGTGATTG CCTGCTTGAA AAATCAGAGC AGAATGACTT TTTCAGTCTG 
AAATGGGATG ACCTTCTCTT AAGGTGTCTA GAGAAGATGC AGCATTCCCA CCAAGTGACC 
TTCCCTGGAC GACCACCTAT TATACGAAAA GGAAACATTG ATCCCATTGA TATCAGTGTG 
GTGCAGAGGG CGTCAAACAA AAAGGTGACA ATGATTAAAA ATCTTGAATT GTATGGACTA 
GATCCCTCTT CAGTTGGGAG CATGCTCCAA CAGCGTGTTC AAGCCAGTTC CACATGCACT 
GCCATTCCAG GGTCTAAGGA CAGAGTGCAG GTGCAGATAC AGGGCAACCA GGTGTCTCAG 
GTTGGAAAAC TACTACTTGA AGAGTTTAAA ATACCCCGCA AATACATTTC TGGATTGGAC 
CTGGCTAAAT CTGGTAAAAA GAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif2d ( XM_012958645.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814099.1 Xenopus tropicalis eukaryotic translation initiation factor 2D (eif2d), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa26190
Clone ID Related Accession (Same CDS sequence)	XM_012958645.3 , XM_012958645.2
Accession Version	XM_012958645.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 2D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958645.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCTA AGAATTTTAG GGTTAAGTCA AATACAGCTA TAAAGGGATC GGACAGGAGG 
AAGCTGCGAG CAGATATCAG TTCTGCATTT CCTTTTCTTA CCACAGAAAA CTTGTCTGAG 
TTGATGCCAT CTAAAGAGGA ACTGAGTATT GTAAAAGTCT TTGCTCACAA AGGAGATGCA 
GTGACATTAT ACGTCCACAA TCGAAACCCA GTTATGTTTG AACTGGAGAA AAACCTTTAT 
CCAACAGTAT ATGCTTTGTG GAAGTATCCA GATCTTCTCC CAGCATTCAC CACATGGCCA 
CCTGTCCTGG AGAAAATGGC AGGTGGAGCA GATCTGATGC TGCCTGGTGT TGTTGTCCCC 
TCATTTGGTC TTCCTGAGAT ACAACAAGGC AGCCTATGTG CAATCACCTT GGTGGGAAAC 
AGGGCACCTG TAGCGGTTGG TGTGGCCACT ATGTCATCGA AAGAGATGCT GGCATCAGGA 
ATGAAAGGGA AAGGTTTCAA TATTTTGCAC ACATTTGGAG ATCAGTTATG GGCATATGGA 
GACAAGTCTT GTTTGCCAAA TATCCCCATA CCGGAGGTCA GTCTAACAGT AGTGGAAGAA 
GGAGATGGGC AAGCTGTGTC CCAGGAGGTC TCCTCCGAAG CCCAGGATCC ACAAGCACAG 
CTGGAGAATC TCAATCTCAT GGAGGAAGAA ACCACACACA AAGAAGAGGA TTCTGAAAAA 
CATTGTGAGG GTGTAGATGA ACAGATTAAA GAGACAGAAA ATTTAGTTCC TTTACAGAAG 
TCGTCTCAAG CTATGGACAG TTTGCTGCAC CAGTGCTTCT TTCATGCATT GAAGCATAAA 
GTGAAAAAAT CCGATCTTCC TCTTCTTACA AGCACATTTC TGCGCAATTA TCTTTTTGCC 
TGCTGTCCAG AAGGACAGCA AGTGGATATT AAAAAATCAA GTTACAAAAA GCTAACTAAG 
TTTTTACAAA CTATGCAACA GCGAAAGGTT TTGCAGGTGA AGGAGCTCAA TAAGGGAGTT 
GAGAGCATAG TGGAAATAGA CTGGAAGCAT CCTGATGTTC GTTCCTTTGT TGCACCAGAG 
CCTGTACCAG TGGACTTGGT AGTTTCTGGT AGCATAGATG GGGGTGGAGA ATCGCCATAC 
CAGCCCCCTG AAATAATAAC CCTCTATGGT ATTTCCAGTA AAATGCTGCC TCTTTTCCAA 
GAGGCAGGGC ATAGAAAAGG AGATCTGCTC ACCCATAGTG ATGTTAGAAG CATCATTATT 
ACTTATGTGA AAAACAATGA GCTTGTTGAT TCAAATAATA AGAACTTTGT GAATATAAAC 
CCAGTTCTGT GTGATTGCCT GCTTGAAAAA TCAGAGCAGA ATGACTTTTT CAGTCTGAAA 
TGGGATGACC TTCTCTTAAG GTGTCTAGAG AAGATGCAGC ATTCCCACCA AGTGACCTTC 
CCTGGACGAC CACCTATTAT ACGAAAAGGA AACATTGATC CCATTGATAT CAGTGTGGTG 
CAGAGGGCGT CAAACAAAAA GGTGACAATG ATTAAAAATC TTGAATTGTA TGGACTAGAT 
CCCTCTTCAG TTGGGAGCAT GCTCCAACAG CGTGTTCAAG CCAGTTCCAC ATGCACTGCC 
ATTCCAGGGT CTAAGGACAG AGTGCAGGTG CAGATACAGG GCAACCAGGT GTCTCAGGTT 
GGAAAACTAC TACTTGAAGA GTTTAAAATA CCCCGCAAAT ACATTTCTGG ATTGGACCTG 
GCTAAATCTG GTAAAAAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814099.1
CDS	199..1962
Translation	MFAKNFRVKSNTAIKGSDRRKLRADISSAFPFLTTENLSELMPSKEELSIVKVFAHKGDAVTLYVHNRNPVMFELEKNLYPTVYALWKYPDLLPAFTTWPPVLEKMAGGADLMLPGVVVPSFGLPEIQQGSLCAITLVGNRAPVAVGVATMSSKEMLASGMKGKGFNILHTFGDQLWAYGDKSCLPNIPIPEVSLTVVEEGDGQAVSQEVSSEAQDPQAQLENLNLMEEETTHKEEDSEKHCEGVDEQIKETENLVPLQKSSQAMDSLLHQCFFHALKHKVKKSDLPLLTSTFLRNYLFACCPEGQQVDIKKSSYKKLTKFLQTMQQRKVLQVKELNKGVESIVEIDWKHPDVRSFVAPEPVPVDLVVSGSIDGGGESPYQPPEIITLYGISSKMLPLFQEAGHRKGDLLTHSDVRSIIITYVKNNELVDSNNKNFVNINPVLCDCLLEKSEQNDFFSLKWDDLLLRCLEKMQHSHQVTFPGRPPIIRKGNIDPIDISVVQRASNKKVTMIKNLELYGLDPSSVGSMLQQRVQASSTCTAIPGSKDRVQVQIQGNQVSQVGKLLLEEFKIPRKYISGLDLAKSGKKK

Target ORF information:

RefSeq Version	XM_012958645.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 2D (eif2d), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958645.2

ORF Insert Sequence:

ATGTTTGCTA AGAATTTTAG GGTTAAGTCA AATACAGCTA TAAAGGGATC GGACAGGAGG 
AAGCTGCGAG CAGATATCAG TTCTGCATTT CCTTTTCTTA CCACAGAAAA CTTGTCTGAG 
TTGATGCCAT CTAAAGAGGA ACTGAGTATT GTAAAAGTCT TTGCTCACAA AGGAGATGCA 
GTGACATTAT ACGTCCACAA TCGAAACCCA GTTATGTTTG AACTGGAGAA AAACCTTTAT 
CCAACAGTAT ATGCTTTGTG GAAGTATCCA GATCTTCTCC CAGCATTCAC CACATGGCCA 
CCTGTCCTGG AGAAAATGGC AGGTGGAGCA GATCTGATGC TGCCTGGTGT TGTTGTCCCC 
TCATTTGGTC TTCCTGAGAT ACAACAAGGC AGCCTATGTG CAATCACCTT GGTGGGAAAC 
AGGGCACCTG TAGCGGTTGG TGTGGCCACT ATGTCATCGA AAGAGATGCT GGCATCAGGA 
ATGAAAGGGA AAGGTTTCAA TATTTTGCAC ACATTTGGAG ATCAGTTATG GGCATATGGA 
GACAAGTCTT GTTTGCCAAA TATCCCCATA CCGGAGGTCA GTCTAACAGT AGTGGAAGAA 
GGAGATGGGC AAGCTGTGTC CCAGGAGGTC TCCTCCGAAG CCCAGGATCC ACAAGCACAG 
CTGGAGAATC TCAATCTCAT GGAGGAAGAA ACCACACACA AAGAAGAGGA TTCTGAAAAA 
CATTGTGAGG GTGTAGATGA ACAGATTAAA GAGACAGAAA ATTTAGTTCC TTTACAGAAG 
TCGTCTCAAG CTATGGACAG TTTGCTGCAC CAGTGCTTCT TTCATGCATT GAAGCATAAA 
GTGAAAAAAT CCGATCTTCC TCTTCTTACA AGCACATTTC TGCGCAATTA TCTTTTTGCC 
TGCTGTCCAG AAGGACAGCA AGTGGATATT AAAAAATCAA GTTACAAAAA GCTAACTAAG 
TTTTTACAAA CTATGCAACA GCGAAAGGTT TTGCAGGTGA AGGAGCTCAA TAAGGGAGTT 
GAGAGCATAG TGGAAATAGA CTGGAAGCAT CCTGATGTTC GTTCCTTTGT TGCACCAGAG 
CCTGTACCAG TGGACTTGGT AGTTTCTGGT AGCATAGATG GGGGTGGAGA ATCGCCATAC 
CAGCCCCCTG AAATAATAAC CCTCTATGGT ATTTCCAGTA AAATGCTGCC TCTTTTCCAA 
GAGGCAGGGC ATAGAAAAGG AGATCTGCTC ACCCATAGTG ATGTTAGAAG CATCATTATT 
ACTTATGTGA AAAACAATGA GCTTGTTGAT TCAAATAATA AGAACTTTGT GAATATAAAC 
CCAGTTCTGT GTGATTGCCT GCTTGAAAAA TCAGAGCAGA ATGACTTTTT CAGTCTGAAA 
TGGGATGACC TTCTCTTAAG GTGTCTAGAG AAGATGCAGC ATTCCCACCA AGTGACCTTC 
CCTGGACGAC CACCTATTAT ACGAAAAGGA AACATTGATC CCATTGATAT CAGTGTGGTG 
CAGAGGGCGT CAAACAAAAA GGTGACAATG ATTAAAAATC TTGAATTGTA TGGACTAGAT 
CCCTCTTCAG TTGGGAGCAT GCTCCAACAG CGTGTTCAAG CCAGTTCCAC ATGCACTGCC 
ATTCCAGGGT CTAAGGACAG AGTGCAGGTG CAGATACAGG GCAACCAGGT GTCTCAGGTT 
GGAAAACTAC TACTTGAAGA GTTTAAAATA CCCCGCAAAT ACATTTCTGG ATTGGACCTG 
GCTAAATCTG GTAAAAAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif2d ( XM_012958652.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814106.1 Xenopus tropicalis eukaryotic translation initiation factor 2D (eif2d), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa26191
Clone ID Related Accession (Same CDS sequence)	XM_012958652.3 , XM_012958652.2
Accession Version	XM_012958652.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 2D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958652.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATCTA AAGAGGAACT GAGTATTGTA AAAGTCTTTG CTCACAAAGG AGATGCAGTG 
ACATTATACG TCCACAATCG AAACCCAGTT ATGTTTGAAC TGGAGAAAAA CCTTTATCCA 
ACAGTATATG CTTTGTGGAA GTATCCAGAT CTTCTCCCAG CATTCACCAC ATGGCCACCT 
GTCCTGGAGA AAATGGCAGG TGGAGCAGAT CTGATGCTGC CTGGTGTTGT TGTCCCCTCA 
TTTGGTCTTC CTGAGATACA ACAAGGCAGC CTATGTGCAA TCACCTTGGT GGGAAACAGG 
GCACCTGTAG CGGTTGGTGT GGCCACTATG TCATCGAAAG AGATGCTGGC ATCAGGAATG 
AAAGGGAAAG GTTTCAATAT TTTGCACACA TTTGGAGATC AGTTATGGGC ATATGGAGAC 
AAGTCTTGTT TGCCAAATAT CCCCATACCG GAGGTCAGTC TAACAGTAGT GGAAGAAGGA 
GATGGGCAAG CTGTGTCCCA GGAGGTCTCC TCCGAAGCCC AGGATCCACA AGCACAGCTG 
GAGAATCTCA ATCTCATGGA GGAAGAAACC ACACACAAAG AAGAGGATTC TGAAAAACAT 
TGTGAGGGTG TAGATGAACA GATTAAAGAG ACAGAAAATT TAGTTCCTTT ACAGAAGTCG 
TCTCAAGAAG CTATGGACAG TTTGCTGCAC CAGTGCTTCT TTCATGCATT GAAGCATAAA 
GTGAAAAAAT CCGATCTTCC TCTTCTTACA AGCACATTTC TGCGCAATTA TCTTTTTGCC 
TGCTGTCCAG AAGGACAGCA AGTGGATATT AAAAAATCAA GTTACAAAAA GCTAACTAAG 
TTTTTACAAA CTATGCAACA GCGAAAGGTT TTGCAGGTGA AGGAGCTCAA TAAGGGAGTT 
GAGAGCATAG TGGAAATAGA CTGGAAGCAT CCTGATGTTC GTTCCTTTGT TGCACCAGAG 
CCTGTACCAG TGGACTTGGT AGTTTCTGGT AGCATAGATG GGGGTGGAGA ATCGCCATAC 
CAGCCCCCTG AAATAATAAC CCTCTATGGT ATTTCCAGTA AAATGCTGCC TCTTTTCCAA 
GAGGCAGGGC ATAGAAAAGG AGATCTGCTC ACCCATAGTG ATGTTAGAAG CATCATTATT 
ACTTATGTGA AAAACAATGA GCTTGTTGAT TCAAATAATA AGAACTTTGT GAATATAAAC 
CCAGTTCTGT GTGATTGCCT GCTTGAAAAA TCAGAGCAGA ATGACTTTTT CAGTCTGAAA 
TGGGATGACC TTCTCTTAAG GTGTCTAGAG AAGATGCAGC ATTCCCACCA AGTGACCTTC 
CCTGGACGAC CACCTATTAT ACGAAAAGGA AACATTGATC CCATTGATAT CAGTGTGGTG 
CAGAGGGCGT CAAACAAAAA GGTGACAATG ATTAAAAATC TTGAATTGTA TGGACTAGAT 
CCCTCTTCAG TTGGGAGCAT GCTCCAACAG CGTGTTCAAG CCAGTTCCAC ATGCACTGCC 
ATTCCAGGGT CTAAGGACAG AGTGCAGGTG CAGATACAGG GCAACCAGGT GTCTCAGGTT 
GGAAAACTAC TACTTGAAGA GTTTAAAATA CCCCGCAAAT ACATTTCTGG ATTGGACCTG 
GCTAAATCTG GTAAAAAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814106.1
CDS	200..1843
Translation	MPSKEELSIVKVFAHKGDAVTLYVHNRNPVMFELEKNLYPTVYALWKYPDLLPAFTTWPPVLEKMAGGADLMLPGVVVPSFGLPEIQQGSLCAITLVGNRAPVAVGVATMSSKEMLASGMKGKGFNILHTFGDQLWAYGDKSCLPNIPIPEVSLTVVEEGDGQAVSQEVSSEAQDPQAQLENLNLMEEETTHKEEDSEKHCEGVDEQIKETENLVPLQKSSQEAMDSLLHQCFFHALKHKVKKSDLPLLTSTFLRNYLFACCPEGQQVDIKKSSYKKLTKFLQTMQQRKVLQVKELNKGVESIVEIDWKHPDVRSFVAPEPVPVDLVVSGSIDGGGESPYQPPEIITLYGISSKMLPLFQEAGHRKGDLLTHSDVRSIIITYVKNNELVDSNNKNFVNINPVLCDCLLEKSEQNDFFSLKWDDLLLRCLEKMQHSHQVTFPGRPPIIRKGNIDPIDISVVQRASNKKVTMIKNLELYGLDPSSVGSMLQQRVQASSTCTAIPGSKDRVQVQIQGNQVSQVGKLLLEEFKIPRKYISGLDLAKSGKKK

Target ORF information:

RefSeq Version	XM_012958652.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 2D (eif2d), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958652.2

ORF Insert Sequence:

ATGCCATCTA AAGAGGAACT GAGTATTGTA AAAGTCTTTG CTCACAAAGG AGATGCAGTG 
ACATTATACG TCCACAATCG AAACCCAGTT ATGTTTGAAC TGGAGAAAAA CCTTTATCCA 
ACAGTATATG CTTTGTGGAA GTATCCAGAT CTTCTCCCAG CATTCACCAC ATGGCCACCT 
GTCCTGGAGA AAATGGCAGG TGGAGCAGAT CTGATGCTGC CTGGTGTTGT TGTCCCCTCA 
TTTGGTCTTC CTGAGATACA ACAAGGCAGC CTATGTGCAA TCACCTTGGT GGGAAACAGG 
GCACCTGTAG CGGTTGGTGT GGCCACTATG TCATCGAAAG AGATGCTGGC ATCAGGAATG 
AAAGGGAAAG GTTTCAATAT TTTGCACACA TTTGGAGATC AGTTATGGGC ATATGGAGAC 
AAGTCTTGTT TGCCAAATAT CCCCATACCG GAGGTCAGTC TAACAGTAGT GGAAGAAGGA 
GATGGGCAAG CTGTGTCCCA GGAGGTCTCC TCCGAAGCCC AGGATCCACA AGCACAGCTG 
GAGAATCTCA ATCTCATGGA GGAAGAAACC ACACACAAAG AAGAGGATTC TGAAAAACAT 
TGTGAGGGTG TAGATGAACA GATTAAAGAG ACAGAAAATT TAGTTCCTTT ACAGAAGTCG 
TCTCAAGAAG CTATGGACAG TTTGCTGCAC CAGTGCTTCT TTCATGCATT GAAGCATAAA 
GTGAAAAAAT CCGATCTTCC TCTTCTTACA AGCACATTTC TGCGCAATTA TCTTTTTGCC 
TGCTGTCCAG AAGGACAGCA AGTGGATATT AAAAAATCAA GTTACAAAAA GCTAACTAAG 
TTTTTACAAA CTATGCAACA GCGAAAGGTT TTGCAGGTGA AGGAGCTCAA TAAGGGAGTT 
GAGAGCATAG TGGAAATAGA CTGGAAGCAT CCTGATGTTC GTTCCTTTGT TGCACCAGAG 
CCTGTACCAG TGGACTTGGT AGTTTCTGGT AGCATAGATG GGGGTGGAGA ATCGCCATAC 
CAGCCCCCTG AAATAATAAC CCTCTATGGT ATTTCCAGTA AAATGCTGCC TCTTTTCCAA 
GAGGCAGGGC ATAGAAAAGG AGATCTGCTC ACCCATAGTG ATGTTAGAAG CATCATTATT 
ACTTATGTGA AAAACAATGA GCTTGTTGAT TCAAATAATA AGAACTTTGT GAATATAAAC 
CCAGTTCTGT GTGATTGCCT GCTTGAAAAA TCAGAGCAGA ATGACTTTTT CAGTCTGAAA 
TGGGATGACC TTCTCTTAAG GTGTCTAGAG AAGATGCAGC ATTCCCACCA AGTGACCTTC 
CCTGGACGAC CACCTATTAT ACGAAAAGGA AACATTGATC CCATTGATAT CAGTGTGGTG 
CAGAGGGCGT CAAACAAAAA GGTGACAATG ATTAAAAATC TTGAATTGTA TGGACTAGAT 
CCCTCTTCAG TTGGGAGCAT GCTCCAACAG CGTGTTCAAG CCAGTTCCAC ATGCACTGCC 
ATTCCAGGGT CTAAGGACAG AGTGCAGGTG CAGATACAGG GCAACCAGGT GTCTCAGGTT 
GGAAAACTAC TACTTGAAGA GTTTAAAATA CCCCGCAAAT ACATTTCTGG ATTGGACCTG 
GCTAAATCTG GTAAAAAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif2d ( XM_012958655.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814109.1 Xenopus tropicalis eukaryotic translation initiation factor 2D (eif2d), transcript variant X3, mRNA.

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CloneID	OXa26192
Clone ID Related Accession (Same CDS sequence)	XM_012958655.2
Accession Version	XM_012958655.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 2D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958655.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCTA AGAATTTTAG GGTTAAGTCA AATACAGCTA TAAAGGGATC GGACAGGAGG 
AAGCTGCGAG CAGATATCAG TTCTGCATTT CCTTTTCTTA CCACAGAAAA CTTGTCTGAG 
TTGATGCCAT CTAAAGAGGA ACTGAGTATT GTAAAAGTCT TTGCTCACAA AGGAGATGCA 
GTGACATTAT ACGTCCACAA TCGAAACCCA GTTATGTTTG AACTGGAGAA AAACCTTTAT 
CCAACAGTAT ATGCTTTGTG GAAGTATCCA GATCTTCTCC CAGCATTCAC CACATGGCCA 
CCTGTCCTGG AGAAAATGGC AGGTGGAGCA GATCTGATGC TGCCTGGTGT TGTTGTCCCC 
TCATTTGGTC TTCCTGAGAT ACAACAAGGC AGCCTATGTG CAATCACCTT GGTGGGAAAC 
AGGGCACCTG TAGCGGTTGG TGTGGCCACT ATGTCATCGA AAGAGATGCT GGCATCAGGA 
ATGAAAGGGA AAGGTTTCAA TATTTTGCAC ACATTTGGAG ATCAGTTATG GGCATATGGA 
GACAAGTCTT GTTTGCCAAA TATCCCCATA CCGGAGGTCA GTCTAACAGT AGTGGAAGAA 
GGAGATGGGC AAGCTGTGTC CCAGGAGGTC TCCTCCGAAG CCCAGGATCC ACAAGCACAG 
CTGGAGAATC TCAATCTCAT GGAGGAAGAA ACCACACACA AAGAAGAGGA TTCTGAAAAA 
CATTGTGAGG GTGTAGATGA ACAGATTAAA GAGACAGAAA ATTTAGTTCC TTTACAGAAG 
TCGTCTCAAG AAGCTATGGA CAGTTTGCTG CACCAGTGCT TCTTTCATGC ATTGAAGCAT 
AAAGTGAAAA AATCCGATCT TCCTCTTCTT ACAAGCACAT TTCTGCGCAA TTATCTTTTT 
GCCTGCTGTC CAGAAGGACA GCAAGTGGAT ATTAAAAAAT CAAGTTACAA AAAGCTAACT 
AAGTTTTTAC AAACTATGCA ACAGCGAAAG GTTTTGCAGG TGAAGGAGCT CAATAAGGGA 
GTTGAGAGCA TAGTGGAAAT AGACTGGAAG CATCCTGAAA AAGGAGATCT GCTCACCCAT 
AGTGATGTTA GAAGCATCAT TATTACTTAT GTGAAAAACA ATGAGCTTGT TGATTCAAAT 
AATAAGAACT TTGTGAATAT AAACCCAGTT CTGTGTGATT GCCTGCTTGA AAAATCAGAG 
CAGAATGACT TTTTCAGTCT GAAATGGGAT GACCTTCTCT TAAGGTGTCT AGAGAAGATG 
CAGCATTCCC ACCAAGTGAC CTTCCCTGGA CGACCACCTA TTATACGAAA AGGAAACATT 
GATCCCATTG ATATCAGTGT GGTGCAGAGG GCGTCAAACA AAAAGGTGAC AATGATTAAA 
AATCTTGAAT TGTATGGACT AGATCCCTCT TCAGTTGGGA GCATGCTCCA ACAGCGTGTT 
CAAGCCAGTT CCACATGCAC TGCCATTCCA GGGTCTAAGG ACAGAGTGCA GGTGCAGATA 
CAGGGCAACC AGGTGTCTCA GGTTGGAAAA CTACTACTTG AAGAGTTTAA AATACCCCGC 
AAATACATTT CTGGATTGGA CCTGGCTAAA TCTGGTAAAA AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814109.1
CDS	199..1806
Translation	MFAKNFRVKSNTAIKGSDRRKLRADISSAFPFLTTENLSELMPSKEELSIVKVFAHKGDAVTLYVHNRNPVMFELEKNLYPTVYALWKYPDLLPAFTTWPPVLEKMAGGADLMLPGVVVPSFGLPEIQQGSLCAITLVGNRAPVAVGVATMSSKEMLASGMKGKGFNILHTFGDQLWAYGDKSCLPNIPIPEVSLTVVEEGDGQAVSQEVSSEAQDPQAQLENLNLMEEETTHKEEDSEKHCEGVDEQIKETENLVPLQKSSQEAMDSLLHQCFFHALKHKVKKSDLPLLTSTFLRNYLFACCPEGQQVDIKKSSYKKLTKFLQTMQQRKVLQVKELNKGVESIVEIDWKHPEKGDLLTHSDVRSIIITYVKNNELVDSNNKNFVNINPVLCDCLLEKSEQNDFFSLKWDDLLLRCLEKMQHSHQVTFPGRPPIIRKGNIDPIDISVVQRASNKKVTMIKNLELYGLDPSSVGSMLQQRVQASSTCTAIPGSKDRVQVQIQGNQVSQVGKLLLEEFKIPRKYISGLDLAKSGKKK

Target ORF information:

RefSeq Version	XM_012958655.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 2D (eif2d), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958655.2

ORF Insert Sequence:

ATGTTTGCTA AGAATTTTAG GGTTAAGTCA AATACAGCTA TAAAGGGATC GGACAGGAGG 
AAGCTGCGAG CAGATATCAG TTCTGCATTT CCTTTTCTTA CCACAGAAAA CTTGTCTGAG 
TTGATGCCAT CTAAAGAGGA ACTGAGTATT GTAAAAGTCT TTGCTCACAA AGGAGATGCA 
GTGACATTAT ACGTCCACAA TCGAAACCCA GTTATGTTTG AACTGGAGAA AAACCTTTAT 
CCAACAGTAT ATGCTTTGTG GAAGTATCCA GATCTTCTCC CAGCATTCAC CACATGGCCA 
CCTGTCCTGG AGAAAATGGC AGGTGGAGCA GATCTGATGC TGCCTGGTGT TGTTGTCCCC 
TCATTTGGTC TTCCTGAGAT ACAACAAGGC AGCCTATGTG CAATCACCTT GGTGGGAAAC 
AGGGCACCTG TAGCGGTTGG TGTGGCCACT ATGTCATCGA AAGAGATGCT GGCATCAGGA 
ATGAAAGGGA AAGGTTTCAA TATTTTGCAC ACATTTGGAG ATCAGTTATG GGCATATGGA 
GACAAGTCTT GTTTGCCAAA TATCCCCATA CCGGAGGTCA GTCTAACAGT AGTGGAAGAA 
GGAGATGGGC AAGCTGTGTC CCAGGAGGTC TCCTCCGAAG CCCAGGATCC ACAAGCACAG 
CTGGAGAATC TCAATCTCAT GGAGGAAGAA ACCACACACA AAGAAGAGGA TTCTGAAAAA 
CATTGTGAGG GTGTAGATGA ACAGATTAAA GAGACAGAAA ATTTAGTTCC TTTACAGAAG 
TCGTCTCAAG AAGCTATGGA CAGTTTGCTG CACCAGTGCT TCTTTCATGC ATTGAAGCAT 
AAAGTGAAAA AATCCGATCT TCCTCTTCTT ACAAGCACAT TTCTGCGCAA TTATCTTTTT 
GCCTGCTGTC CAGAAGGACA GCAAGTGGAT ATTAAAAAAT CAAGTTACAA AAAGCTAACT 
AAGTTTTTAC AAACTATGCA ACAGCGAAAG GTTTTGCAGG TGAAGGAGCT CAATAAGGGA 
GTTGAGAGCA TAGTGGAAAT AGACTGGAAG CATCCTGAAA AAGGAGATCT GCTCACCCAT 
AGTGATGTTA GAAGCATCAT TATTACTTAT GTGAAAAACA ATGAGCTTGT TGATTCAAAT 
AATAAGAACT TTGTGAATAT AAACCCAGTT CTGTGTGATT GCCTGCTTGA AAAATCAGAG 
CAGAATGACT TTTTCAGTCT GAAATGGGAT GACCTTCTCT TAAGGTGTCT AGAGAAGATG 
CAGCATTCCC ACCAAGTGAC CTTCCCTGGA CGACCACCTA TTATACGAAA AGGAAACATT 
GATCCCATTG ATATCAGTGT GGTGCAGAGG GCGTCAAACA AAAAGGTGAC AATGATTAAA 
AATCTTGAAT TGTATGGACT AGATCCCTCT TCAGTTGGGA GCATGCTCCA ACAGCGTGTT 
CAAGCCAGTT CCACATGCAC TGCCATTCCA GGGTCTAAGG ACAGAGTGCA GGTGCAGATA 
CAGGGCAACC AGGTGTCTCA GGTTGGAAAA CTACTACTTG AAGAGTTTAA AATACCCCGC 
AAATACATTT CTGGATTGGA CCTGGCTAAA TCTGGTAAAA AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif2d ( XM_018091152.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946641.1 Xenopus tropicalis eukaryotic translation initiation factor 2D (eif2d), transcript variant X4, mRNA.

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CloneID	OXa42493
Clone ID Related Accession (Same CDS sequence)	XM_018091152.1
Accession Version	XM_018091152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 2D isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCTA AGAATTTTAG GGTTAAGTCA AATACAGCTA TAAAGGGATC GGACAGGAGG 
AAGCTGCGAG CAGATATCAG TTCTGCATTT CCTTTTCTTA CCACAGAAAA CTTGTCTGAG 
TTGATGCCAT CTAAAGAGGA ACTGAGTATT GTAAAAGTCT TTGCTCACAA AGGAGATGCA 
GTGACATTAT ACGTCCACAA TCGAAACCCA GTTATGTTTG AACTGGAGAA AAACCTTTAT 
CCAACAGTAT ATGCTTTGTG GAAGTATCCA GATCTTCTCC CAGCATTCAC CACATGGCCA 
CCTGTCCTGG AGAAAATGGC AGGTGGAGCA GATCTGATGC TGCCTGGTGT TGTTGTCCCC 
TCATTTGGTC TTCCTGAGAT ACAACAAGGC AGCCTATGTG CAATCACCTT GGTGGGAAAC 
AGGGCACCTG TAGCGGTTGG TGTGGCCACT ATGTCATCGA AAGAGATGCT GGCATCAGGA 
ATGAAAGGGA AAGGTTTCAA TATTTTGCAC ACATTTGGAG ATCAGTTATG GGCATATGGA 
GACAAGTCTT GTTTGCCAAA TATCCCCATA CCGGAGGTCA GTCTAACAGT AGTGGAAGAA 
GGAGATGGGC AAGCTGTGTC CCAGGAGGTC TCCTCCGAAG CCCAGGATCC ACAAGCACAG 
CTGGAGAATC TCAATCTCAT GGAGGAAGAA ACCACACACA AAGAAGAGGA TTCTGAAAAA 
CATTGTGAGG GTGTAGATGA ACAGATTAAA GAGACAGAAA ATTTAGTTCC TTTACAGAAG 
TCGTCTCAAG AAGCTATGGA CAGTTTGCTG CACCAGTGCT TCTTTCATGC ATTGAAGCAT 
AAAGTGAAAA AATCCGATCT TCCTCTTCTT ACAAGCACAT TTCTGCGCAA TTATCTTTTT 
GCCTGCTGTC CAGAAGGACA GCAAGTGGAT ATTAAAAAAT CAAGTTACAA AAAGCTAACT 
AAGTTTTTAC AAACTATGCA ACAGCGAAAG GTTTTGCAGG TGAAGGAGCT CAATAAGGGA 
GTTGAGAGCA TAGTGGAAAT AGACTGGAAG CATCCTGATG TTCGTTCCTT TGTTGCACCA 
GAGCCTGTAC CAGTGGACTT GGTAGTTTCT GGTAGCATAG ATGGGGGTGG AGAATCGCCA 
TACCAGCCCC CTGAAATAAT AACCCTCTAT GGTATTTCCA GTAAAATGCT GCCTCTTTTC 
CAAGAGGCAG GGCATAGAAA AGGAGATCTG CTCACCCATA GTGATGTTAG AAGCATCATT 
ATTACTTATG TGAAAAACAA TGAGCTTGTT GATTCAAATA ATAAGAACTT TGTGAATATA 
AACCCAGTTC TGTGTGATTG CCTGCTTGAA AAATCAGAGC AGAATGACTT TTTCAGTCTG 
AAATGGGATG ACCTTCTCTT AAGTGTAATA AATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946641.1
CDS	206..1624
Translation	MFAKNFRVKSNTAIKGSDRRKLRADISSAFPFLTTENLSELMPSKEELSIVKVFAHKGDAVTLYVHNRNPVMFELEKNLYPTVYALWKYPDLLPAFTTWPPVLEKMAGGADLMLPGVVVPSFGLPEIQQGSLCAITLVGNRAPVAVGVATMSSKEMLASGMKGKGFNILHTFGDQLWAYGDKSCLPNIPIPEVSLTVVEEGDGQAVSQEVSSEAQDPQAQLENLNLMEEETTHKEEDSEKHCEGVDEQIKETENLVPLQKSSQEAMDSLLHQCFFHALKHKVKKSDLPLLTSTFLRNYLFACCPEGQQVDIKKSSYKKLTKFLQTMQQRKVLQVKELNKGVESIVEIDWKHPDVRSFVAPEPVPVDLVVSGSIDGGGESPYQPPEIITLYGISSKMLPLFQEAGHRKGDLLTHSDVRSIIITYVKNNELVDSNNKNFVNINPVLCDCLLEKSEQNDFFSLKWDDLLLSVINF

Target ORF information:

RefSeq Version	XM_018091152.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 2D (eif2d), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091152.1

ORF Insert Sequence:

ATGTTTGCTA AGAATTTTAG GGTTAAGTCA AATACAGCTA TAAAGGGATC GGACAGGAGG 
AAGCTGCGAG CAGATATCAG TTCTGCATTT CCTTTTCTTA CCACAGAAAA CTTGTCTGAG 
TTGATGCCAT CTAAAGAGGA ACTGAGTATT GTAAAAGTCT TTGCTCACAA AGGAGATGCA 
GTGACATTAT ACGTCCACAA TCGAAACCCA GTTATGTTTG AACTGGAGAA AAACCTTTAT 
CCAACAGTAT ATGCTTTGTG GAAGTATCCA GATCTTCTCC CAGCATTCAC CACATGGCCA 
CCTGTCCTGG AGAAAATGGC AGGTGGAGCA GATCTGATGC TGCCTGGTGT TGTTGTCCCC 
TCATTTGGTC TTCCTGAGAT ACAACAAGGC AGCCTATGTG CAATCACCTT GGTGGGAAAC 
AGGGCACCTG TAGCGGTTGG TGTGGCCACT ATGTCATCGA AAGAGATGCT GGCATCAGGA 
ATGAAAGGGA AAGGTTTCAA TATTTTGCAC ACATTTGGAG ATCAGTTATG GGCATATGGA 
GACAAGTCTT GTTTGCCAAA TATCCCCATA CCGGAGGTCA GTCTAACAGT AGTGGAAGAA 
GGAGATGGGC AAGCTGTGTC CCAGGAGGTC TCCTCCGAAG CCCAGGATCC ACAAGCACAG 
CTGGAGAATC TCAATCTCAT GGAGGAAGAA ACCACACACA AAGAAGAGGA TTCTGAAAAA 
CATTGTGAGG GTGTAGATGA ACAGATTAAA GAGACAGAAA ATTTAGTTCC TTTACAGAAG 
TCGTCTCAAG AAGCTATGGA CAGTTTGCTG CACCAGTGCT TCTTTCATGC ATTGAAGCAT 
AAAGTGAAAA AATCCGATCT TCCTCTTCTT ACAAGCACAT TTCTGCGCAA TTATCTTTTT 
GCCTGCTGTC CAGAAGGACA GCAAGTGGAT ATTAAAAAAT CAAGTTACAA AAAGCTAACT 
AAGTTTTTAC AAACTATGCA ACAGCGAAAG GTTTTGCAGG TGAAGGAGCT CAATAAGGGA 
GTTGAGAGCA TAGTGGAAAT AGACTGGAAG CATCCTGATG TTCGTTCCTT TGTTGCACCA 
GAGCCTGTAC CAGTGGACTT GGTAGTTTCT GGTAGCATAG ATGGGGGTGG AGAATCGCCA 
TACCAGCCCC CTGAAATAAT AACCCTCTAT GGTATTTCCA GTAAAATGCT GCCTCTTTTC 
CAAGAGGCAG GGCATAGAAA AGGAGATCTG CTCACCCATA GTGATGTTAG AAGCATCATT 
ATTACTTATG TGAAAAACAA TGAGCTTGTT GATTCAAATA ATAAGAACTT TGTGAATATA 
AACCCAGTTC TGTGTGATTG CCTGCTTGAA AAATCAGAGC AGAATGACTT TTTCAGTCTG 
AAATGGGATG ACCTTCTCTT AAGTGTAATA AATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif2d ( XM_012958645.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814099.1 Xenopus tropicalis eukaryotic translation initiation factor 2D (eif2d), transcript variant X1, mRNA.

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CloneID	OXa26190
Clone ID Related Accession (Same CDS sequence)	XM_012958645.3 , XM_012958645.2
Accession Version	XM_012958645.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 2D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958645.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCTA AGAATTTTAG GGTTAAGTCA AATACAGCTA TAAAGGGATC GGACAGGAGG 
AAGCTGCGAG CAGATATCAG TTCTGCATTT CCTTTTCTTA CCACAGAAAA CTTGTCTGAG 
TTGATGCCAT CTAAAGAGGA ACTGAGTATT GTAAAAGTCT TTGCTCACAA AGGAGATGCA 
GTGACATTAT ACGTCCACAA TCGAAACCCA GTTATGTTTG AACTGGAGAA AAACCTTTAT 
CCAACAGTAT ATGCTTTGTG GAAGTATCCA GATCTTCTCC CAGCATTCAC CACATGGCCA 
CCTGTCCTGG AGAAAATGGC AGGTGGAGCA GATCTGATGC TGCCTGGTGT TGTTGTCCCC 
TCATTTGGTC TTCCTGAGAT ACAACAAGGC AGCCTATGTG CAATCACCTT GGTGGGAAAC 
AGGGCACCTG TAGCGGTTGG TGTGGCCACT ATGTCATCGA AAGAGATGCT GGCATCAGGA 
ATGAAAGGGA AAGGTTTCAA TATTTTGCAC ACATTTGGAG ATCAGTTATG GGCATATGGA 
GACAAGTCTT GTTTGCCAAA TATCCCCATA CCGGAGGTCA GTCTAACAGT AGTGGAAGAA 
GGAGATGGGC AAGCTGTGTC CCAGGAGGTC TCCTCCGAAG CCCAGGATCC ACAAGCACAG 
CTGGAGAATC TCAATCTCAT GGAGGAAGAA ACCACACACA AAGAAGAGGA TTCTGAAAAA 
CATTGTGAGG GTGTAGATGA ACAGATTAAA GAGACAGAAA ATTTAGTTCC TTTACAGAAG 
TCGTCTCAAG CTATGGACAG TTTGCTGCAC CAGTGCTTCT TTCATGCATT GAAGCATAAA 
GTGAAAAAAT CCGATCTTCC TCTTCTTACA AGCACATTTC TGCGCAATTA TCTTTTTGCC 
TGCTGTCCAG AAGGACAGCA AGTGGATATT AAAAAATCAA GTTACAAAAA GCTAACTAAG 
TTTTTACAAA CTATGCAACA GCGAAAGGTT TTGCAGGTGA AGGAGCTCAA TAAGGGAGTT 
GAGAGCATAG TGGAAATAGA CTGGAAGCAT CCTGATGTTC GTTCCTTTGT TGCACCAGAG 
CCTGTACCAG TGGACTTGGT AGTTTCTGGT AGCATAGATG GGGGTGGAGA ATCGCCATAC 
CAGCCCCCTG AAATAATAAC CCTCTATGGT ATTTCCAGTA AAATGCTGCC TCTTTTCCAA 
GAGGCAGGGC ATAGAAAAGG AGATCTGCTC ACCCATAGTG ATGTTAGAAG CATCATTATT 
ACTTATGTGA AAAACAATGA GCTTGTTGAT TCAAATAATA AGAACTTTGT GAATATAAAC 
CCAGTTCTGT GTGATTGCCT GCTTGAAAAA TCAGAGCAGA ATGACTTTTT CAGTCTGAAA 
TGGGATGACC TTCTCTTAAG GTGTCTAGAG AAGATGCAGC ATTCCCACCA AGTGACCTTC 
CCTGGACGAC CACCTATTAT ACGAAAAGGA AACATTGATC CCATTGATAT CAGTGTGGTG 
CAGAGGGCGT CAAACAAAAA GGTGACAATG ATTAAAAATC TTGAATTGTA TGGACTAGAT 
CCCTCTTCAG TTGGGAGCAT GCTCCAACAG CGTGTTCAAG CCAGTTCCAC ATGCACTGCC 
ATTCCAGGGT CTAAGGACAG AGTGCAGGTG CAGATACAGG GCAACCAGGT GTCTCAGGTT 
GGAAAACTAC TACTTGAAGA GTTTAAAATA CCCCGCAAAT ACATTTCTGG ATTGGACCTG 
GCTAAATCTG GTAAAAAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814099.1
CDS	161..1924
Translation	MFAKNFRVKSNTAIKGSDRRKLRADISSAFPFLTTENLSELMPSKEELSIVKVFAHKGDAVTLYVHNRNPVMFELEKNLYPTVYALWKYPDLLPAFTTWPPVLEKMAGGADLMLPGVVVPSFGLPEIQQGSLCAITLVGNRAPVAVGVATMSSKEMLASGMKGKGFNILHTFGDQLWAYGDKSCLPNIPIPEVSLTVVEEGDGQAVSQEVSSEAQDPQAQLENLNLMEEETTHKEEDSEKHCEGVDEQIKETENLVPLQKSSQAMDSLLHQCFFHALKHKVKKSDLPLLTSTFLRNYLFACCPEGQQVDIKKSSYKKLTKFLQTMQQRKVLQVKELNKGVESIVEIDWKHPDVRSFVAPEPVPVDLVVSGSIDGGGESPYQPPEIITLYGISSKMLPLFQEAGHRKGDLLTHSDVRSIIITYVKNNELVDSNNKNFVNINPVLCDCLLEKSEQNDFFSLKWDDLLLRCLEKMQHSHQVTFPGRPPIIRKGNIDPIDISVVQRASNKKVTMIKNLELYGLDPSSVGSMLQQRVQASSTCTAIPGSKDRVQVQIQGNQVSQVGKLLLEEFKIPRKYISGLDLAKSGKKK

Target ORF information:

RefSeq Version	XM_012958645.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 2D (eif2d), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958645.3

ORF Insert Sequence:

ATGTTTGCTA AGAATTTTAG GGTTAAGTCA AATACAGCTA TAAAGGGATC GGACAGGAGG 
AAGCTGCGAG CAGATATCAG TTCTGCATTT CCTTTTCTTA CCACAGAAAA CTTGTCTGAG 
TTGATGCCAT CTAAAGAGGA ACTGAGTATT GTAAAAGTCT TTGCTCACAA AGGAGATGCA 
GTGACATTAT ACGTCCACAA TCGAAACCCA GTTATGTTTG AACTGGAGAA AAACCTTTAT 
CCAACAGTAT ATGCTTTGTG GAAGTATCCA GATCTTCTCC CAGCATTCAC CACATGGCCA 
CCTGTCCTGG AGAAAATGGC AGGTGGAGCA GATCTGATGC TGCCTGGTGT TGTTGTCCCC 
TCATTTGGTC TTCCTGAGAT ACAACAAGGC AGCCTATGTG CAATCACCTT GGTGGGAAAC 
AGGGCACCTG TAGCGGTTGG TGTGGCCACT ATGTCATCGA AAGAGATGCT GGCATCAGGA 
ATGAAAGGGA AAGGTTTCAA TATTTTGCAC ACATTTGGAG ATCAGTTATG GGCATATGGA 
GACAAGTCTT GTTTGCCAAA TATCCCCATA CCGGAGGTCA GTCTAACAGT AGTGGAAGAA 
GGAGATGGGC AAGCTGTGTC CCAGGAGGTC TCCTCCGAAG CCCAGGATCC ACAAGCACAG 
CTGGAGAATC TCAATCTCAT GGAGGAAGAA ACCACACACA AAGAAGAGGA TTCTGAAAAA 
CATTGTGAGG GTGTAGATGA ACAGATTAAA GAGACAGAAA ATTTAGTTCC TTTACAGAAG 
TCGTCTCAAG CTATGGACAG TTTGCTGCAC CAGTGCTTCT TTCATGCATT GAAGCATAAA 
GTGAAAAAAT CCGATCTTCC TCTTCTTACA AGCACATTTC TGCGCAATTA TCTTTTTGCC 
TGCTGTCCAG AAGGACAGCA AGTGGATATT AAAAAATCAA GTTACAAAAA GCTAACTAAG 
TTTTTACAAA CTATGCAACA GCGAAAGGTT TTGCAGGTGA AGGAGCTCAA TAAGGGAGTT 
GAGAGCATAG TGGAAATAGA CTGGAAGCAT CCTGATGTTC GTTCCTTTGT TGCACCAGAG 
CCTGTACCAG TGGACTTGGT AGTTTCTGGT AGCATAGATG GGGGTGGAGA ATCGCCATAC 
CAGCCCCCTG AAATAATAAC CCTCTATGGT ATTTCCAGTA AAATGCTGCC TCTTTTCCAA 
GAGGCAGGGC ATAGAAAAGG AGATCTGCTC ACCCATAGTG ATGTTAGAAG CATCATTATT 
ACTTATGTGA AAAACAATGA GCTTGTTGAT TCAAATAATA AGAACTTTGT GAATATAAAC 
CCAGTTCTGT GTGATTGCCT GCTTGAAAAA TCAGAGCAGA ATGACTTTTT CAGTCTGAAA 
TGGGATGACC TTCTCTTAAG GTGTCTAGAG AAGATGCAGC ATTCCCACCA AGTGACCTTC 
CCTGGACGAC CACCTATTAT ACGAAAAGGA AACATTGATC CCATTGATAT CAGTGTGGTG 
CAGAGGGCGT CAAACAAAAA GGTGACAATG ATTAAAAATC TTGAATTGTA TGGACTAGAT 
CCCTCTTCAG TTGGGAGCAT GCTCCAACAG CGTGTTCAAG CCAGTTCCAC ATGCACTGCC 
ATTCCAGGGT CTAAGGACAG AGTGCAGGTG CAGATACAGG GCAACCAGGT GTCTCAGGTT 
GGAAAACTAC TACTTGAAGA GTTTAAAATA CCCCGCAAAT ACATTTCTGG ATTGGACCTG 
GCTAAATCTG GTAAAAAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif2d ( XM_012958652.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814106.1 Xenopus tropicalis eukaryotic translation initiation factor 2D (eif2d), transcript variant X2, mRNA.

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CloneID	OXa26191
Clone ID Related Accession (Same CDS sequence)	XM_012958652.3 , XM_012958652.2
Accession Version	XM_012958652.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 2D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958652.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATCTA AAGAGGAACT GAGTATTGTA AAAGTCTTTG CTCACAAAGG AGATGCAGTG 
ACATTATACG TCCACAATCG AAACCCAGTT ATGTTTGAAC TGGAGAAAAA CCTTTATCCA 
ACAGTATATG CTTTGTGGAA GTATCCAGAT CTTCTCCCAG CATTCACCAC ATGGCCACCT 
GTCCTGGAGA AAATGGCAGG TGGAGCAGAT CTGATGCTGC CTGGTGTTGT TGTCCCCTCA 
TTTGGTCTTC CTGAGATACA ACAAGGCAGC CTATGTGCAA TCACCTTGGT GGGAAACAGG 
GCACCTGTAG CGGTTGGTGT GGCCACTATG TCATCGAAAG AGATGCTGGC ATCAGGAATG 
AAAGGGAAAG GTTTCAATAT TTTGCACACA TTTGGAGATC AGTTATGGGC ATATGGAGAC 
AAGTCTTGTT TGCCAAATAT CCCCATACCG GAGGTCAGTC TAACAGTAGT GGAAGAAGGA 
GATGGGCAAG CTGTGTCCCA GGAGGTCTCC TCCGAAGCCC AGGATCCACA AGCACAGCTG 
GAGAATCTCA ATCTCATGGA GGAAGAAACC ACACACAAAG AAGAGGATTC TGAAAAACAT 
TGTGAGGGTG TAGATGAACA GATTAAAGAG ACAGAAAATT TAGTTCCTTT ACAGAAGTCG 
TCTCAAGAAG CTATGGACAG TTTGCTGCAC CAGTGCTTCT TTCATGCATT GAAGCATAAA 
GTGAAAAAAT CCGATCTTCC TCTTCTTACA AGCACATTTC TGCGCAATTA TCTTTTTGCC 
TGCTGTCCAG AAGGACAGCA AGTGGATATT AAAAAATCAA GTTACAAAAA GCTAACTAAG 
TTTTTACAAA CTATGCAACA GCGAAAGGTT TTGCAGGTGA AGGAGCTCAA TAAGGGAGTT 
GAGAGCATAG TGGAAATAGA CTGGAAGCAT CCTGATGTTC GTTCCTTTGT TGCACCAGAG 
CCTGTACCAG TGGACTTGGT AGTTTCTGGT AGCATAGATG GGGGTGGAGA ATCGCCATAC 
CAGCCCCCTG AAATAATAAC CCTCTATGGT ATTTCCAGTA AAATGCTGCC TCTTTTCCAA 
GAGGCAGGGC ATAGAAAAGG AGATCTGCTC ACCCATAGTG ATGTTAGAAG CATCATTATT 
ACTTATGTGA AAAACAATGA GCTTGTTGAT TCAAATAATA AGAACTTTGT GAATATAAAC 
CCAGTTCTGT GTGATTGCCT GCTTGAAAAA TCAGAGCAGA ATGACTTTTT CAGTCTGAAA 
TGGGATGACC TTCTCTTAAG GTGTCTAGAG AAGATGCAGC ATTCCCACCA AGTGACCTTC 
CCTGGACGAC CACCTATTAT ACGAAAAGGA AACATTGATC CCATTGATAT CAGTGTGGTG 
CAGAGGGCGT CAAACAAAAA GGTGACAATG ATTAAAAATC TTGAATTGTA TGGACTAGAT 
CCCTCTTCAG TTGGGAGCAT GCTCCAACAG CGTGTTCAAG CCAGTTCCAC ATGCACTGCC 
ATTCCAGGGT CTAAGGACAG AGTGCAGGTG CAGATACAGG GCAACCAGGT GTCTCAGGTT 
GGAAAACTAC TACTTGAAGA GTTTAAAATA CCCCGCAAAT ACATTTCTGG ATTGGACCTG 
GCTAAATCTG GTAAAAAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814106.1
CDS	166..1809
Translation	MPSKEELSIVKVFAHKGDAVTLYVHNRNPVMFELEKNLYPTVYALWKYPDLLPAFTTWPPVLEKMAGGADLMLPGVVVPSFGLPEIQQGSLCAITLVGNRAPVAVGVATMSSKEMLASGMKGKGFNILHTFGDQLWAYGDKSCLPNIPIPEVSLTVVEEGDGQAVSQEVSSEAQDPQAQLENLNLMEEETTHKEEDSEKHCEGVDEQIKETENLVPLQKSSQEAMDSLLHQCFFHALKHKVKKSDLPLLTSTFLRNYLFACCPEGQQVDIKKSSYKKLTKFLQTMQQRKVLQVKELNKGVESIVEIDWKHPDVRSFVAPEPVPVDLVVSGSIDGGGESPYQPPEIITLYGISSKMLPLFQEAGHRKGDLLTHSDVRSIIITYVKNNELVDSNNKNFVNINPVLCDCLLEKSEQNDFFSLKWDDLLLRCLEKMQHSHQVTFPGRPPIIRKGNIDPIDISVVQRASNKKVTMIKNLELYGLDPSSVGSMLQQRVQASSTCTAIPGSKDRVQVQIQGNQVSQVGKLLLEEFKIPRKYISGLDLAKSGKKK

Target ORF information:

RefSeq Version	XM_012958652.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 2D (eif2d), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958652.3

ORF Insert Sequence:

ATGCCATCTA AAGAGGAACT GAGTATTGTA AAAGTCTTTG CTCACAAAGG AGATGCAGTG 
ACATTATACG TCCACAATCG AAACCCAGTT ATGTTTGAAC TGGAGAAAAA CCTTTATCCA 
ACAGTATATG CTTTGTGGAA GTATCCAGAT CTTCTCCCAG CATTCACCAC ATGGCCACCT 
GTCCTGGAGA AAATGGCAGG TGGAGCAGAT CTGATGCTGC CTGGTGTTGT TGTCCCCTCA 
TTTGGTCTTC CTGAGATACA ACAAGGCAGC CTATGTGCAA TCACCTTGGT GGGAAACAGG 
GCACCTGTAG CGGTTGGTGT GGCCACTATG TCATCGAAAG AGATGCTGGC ATCAGGAATG 
AAAGGGAAAG GTTTCAATAT TTTGCACACA TTTGGAGATC AGTTATGGGC ATATGGAGAC 
AAGTCTTGTT TGCCAAATAT CCCCATACCG GAGGTCAGTC TAACAGTAGT GGAAGAAGGA 
GATGGGCAAG CTGTGTCCCA GGAGGTCTCC TCCGAAGCCC AGGATCCACA AGCACAGCTG 
GAGAATCTCA ATCTCATGGA GGAAGAAACC ACACACAAAG AAGAGGATTC TGAAAAACAT 
TGTGAGGGTG TAGATGAACA GATTAAAGAG ACAGAAAATT TAGTTCCTTT ACAGAAGTCG 
TCTCAAGAAG CTATGGACAG TTTGCTGCAC CAGTGCTTCT TTCATGCATT GAAGCATAAA 
GTGAAAAAAT CCGATCTTCC TCTTCTTACA AGCACATTTC TGCGCAATTA TCTTTTTGCC 
TGCTGTCCAG AAGGACAGCA AGTGGATATT AAAAAATCAA GTTACAAAAA GCTAACTAAG 
TTTTTACAAA CTATGCAACA GCGAAAGGTT TTGCAGGTGA AGGAGCTCAA TAAGGGAGTT 
GAGAGCATAG TGGAAATAGA CTGGAAGCAT CCTGATGTTC GTTCCTTTGT TGCACCAGAG 
CCTGTACCAG TGGACTTGGT AGTTTCTGGT AGCATAGATG GGGGTGGAGA ATCGCCATAC 
CAGCCCCCTG AAATAATAAC CCTCTATGGT ATTTCCAGTA AAATGCTGCC TCTTTTCCAA 
GAGGCAGGGC ATAGAAAAGG AGATCTGCTC ACCCATAGTG ATGTTAGAAG CATCATTATT 
ACTTATGTGA AAAACAATGA GCTTGTTGAT TCAAATAATA AGAACTTTGT GAATATAAAC 
CCAGTTCTGT GTGATTGCCT GCTTGAAAAA TCAGAGCAGA ATGACTTTTT CAGTCTGAAA 
TGGGATGACC TTCTCTTAAG GTGTCTAGAG AAGATGCAGC ATTCCCACCA AGTGACCTTC 
CCTGGACGAC CACCTATTAT ACGAAAAGGA AACATTGATC CCATTGATAT CAGTGTGGTG 
CAGAGGGCGT CAAACAAAAA GGTGACAATG ATTAAAAATC TTGAATTGTA TGGACTAGAT 
CCCTCTTCAG TTGGGAGCAT GCTCCAACAG CGTGTTCAAG CCAGTTCCAC ATGCACTGCC 
ATTCCAGGGT CTAAGGACAG AGTGCAGGTG CAGATACAGG GCAACCAGGT GTCTCAGGTT 
GGAAAACTAC TACTTGAAGA GTTTAAAATA CCCCGCAAAT ACATTTCTGG ATTGGACCTG 
GCTAAATCTG GTAAAAAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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eif2d cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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