col18a1 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012970904.2	XP_012826358.1	collagen alpha-1(XVIII) chain isoform X2
XM_012970906.2	XP_012826360.1	collagen alpha-1(XVIII) chain isoform X4
XM_012970907.2	XP_012826361.1	collagen alpha-1(XVIII) chain isoform X5
XM_012970905.1	XP_012826359.1	collagen alpha-1(XVIII) chain isoform X3
XM_012970906.3	XP_012826360.2	collagen alpha-1(XVIII) chain isoform X4
XM_012970907.3	XP_012826361.2	collagen alpha-1(XVIII) chain isoform X5
XM_012970904.3	XP_012826358.2	collagen alpha-1(XVIII) chain isoform X2
XM_012970903.2	XP_012826357.1	collagen alpha-1(XVIII) chain isoform X1
XM_018097368.1	XP_017952857.1	collagen alpha-1(XVIII) chain isoform X6
XM_012970905.2	XP_012826359.2	collagen alpha-1(XVIII) chain isoform X3
XM_012970903.3	XP_012826357.2	collagen alpha-1(XVIII) chain isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa66572	XM_012970906.3 Latest version!	Xenopus tropicalis collagen type XVIII alpha 1 (col18a1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa66573	XM_012970907.3 Latest version!	Xenopus tropicalis collagen type XVIII alpha 1 (col18a1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa66569	XM_012970903.3 Latest version!	Xenopus tropicalis collagen type XVIII alpha 1 (col18a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa66570	XM_012970904.3 Latest version!	Xenopus tropicalis collagen type XVIII alpha 1 (col18a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa66571	XM_012970905.2 Latest version!	Xenopus tropicalis collagen type XVIII alpha 1 (col18a1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa21931	XM_012970907.2 Latest version!	Xenopus tropicalis collagen, type XVIII, alpha 1 (col18a1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OXa26097	XM_012970903.2 Latest version!	Xenopus tropicalis collagen, type XVIII, alpha 1 (col18a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa26098	XM_012970904.2 Latest version!	Xenopus tropicalis collagen, type XVIII, alpha 1 (col18a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa26099	XM_012970905.1 Latest version!	Xenopus tropicalis collagen, type XVIII, alpha 1 (col18a1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa26100	XM_012970906.2 Latest version!	Xenopus tropicalis collagen, type XVIII, alpha 1 (col18a1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OXa46789	XM_018097368.1 Latest version!	Xenopus tropicalis collagen, type XVIII, alpha 1 (col18a1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

col18a1 ( XM_012970906.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826360.2 Xenopus tropicalis collagen type XVIII alpha 1 (col18a1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa66572
Clone ID Related Accession (Same CDS sequence)	XM_012970906.3
Accession Version	XM_012970906.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(XVIII) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970906.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCTCA GGTGTACGTG GGTCCTGTTG CTCTACGCAT CGTATCATGT TCTTCTGGTC 
TCCTCCCAAG GCAAAGACAA CTTCAACCCA GAAGTTGGCC TCTTGCAGTT GATTGGAGAA 
CCTGTACCTG AACAGATCAC CAAGGTCTTT GGGCCCGACA AGACTCCCGC CTATGTATTT 
GGTAAAGAAG CCAATACAGG CCAGGTGGCC CAATACCATC TGCCCAACCC TATGTATAGG 
GACTTCTCTC TCCTCTTCCA TGTCCAGCCA ACCACAGACA AGGCCGGCAT CCTCTTTGCC 
ATCACAGATG CCTCTCAGTC TATTGTGTAT ATCGGGGTGA AGCTTTCTGA AGTCAAGGAT 
GGGAAGCAAG ACATCATCTT CTACTATACA GAGCCTGGCT CACAGGATTC CTATGTAGCT 
GCCACCTTCA ATGTCCCATC ACTGGTGAAT CGTTGGTCTC GCTTTGCAAT CAGTGTGGAG 
GAAGAAGAAG TTGTGCTGTT TATGGATTGT GATGAGCTGG AAAGGGTGCG CTTCGAGAGA 
TCACCTGATG AGATGGAACT AGAAGATGGC TCTGGGCTCT TTGTTGCTCA AGCAGGTGGC 
GCTGATCCAG ACAGATATCA GGGTGTAATA GCAGATCTAA AGATCGTAGC CGATCCAAGA 
GCCGCAGAAC ATCAGTGTGA GGAGGATGGA GATGACTCTG ATGGGATCTC TGGAGATTTT 
GGCAGTGGCA TTGCGGACAA TGATTTGGTG ACTTCTCCTC CTGTGATAAT CAAAGTGCAG 
CCTCCTATAA CTGTCCCTCC CATACAGAGG ACCCCTGTGG AGAAAGAGAT TGTTCAGACA 
GGTCAAGGCC AGCAAGGGGT AAAAGGAGAG AAGGGTGATC GTGGAGAGAA GGGTGAGAGG 
GGACTGACTG GCCCTAAGGG AGACGGTGGG AATGCAAGTC CAACTTCTGA TTCCAAAGGA 
GAAAAGGGTC AGAAAGGAGA GGTCGGAGTG AAGGGAAGTG CAGGCTTTGG CTATCCCGGG 
CCTAAAGGGC AGAAAGGAGA TCCTGGAGAC TTGGGACCAA GAGGACCACC AGGCCCACCT 
GGACCATCTA TATTACAGAA AAGTGATGGA ACTACAGTGC AACAGGTCAC AGGACCTCCA 
GGTCCTCAAG GCCCTGCGGG ACCACCAGGA AAAGAAGGGG AACCAGGTGA CCCTGGTGAA 
GATGGAAAAA CTGGCGAGGT TGGTCCCCAA GGATTTCCAG GGACCCCAGG AGATTCTGGG 
CAGAAGGGAG ACAAGGGTGA TTCTGGTGTT GGTATCAGAG GTGCTCCTGG GCCACCAGGT 
CCGCCAGGCC CCCCTGGACC TTCTAGAACC GACAAGCTGA CATTCATTGA CATGGAAGGT 
TCTGGATATG GTGGAGATTC GGAGCACATC AGGGGAGCCC CTGGTGTGCC TGGGCCCCCA 
GGTCCTCCAG GTGTACCAGG GTTACCCGGA GTACCCGGAA CTTTTGGCAT GAACAACACA 
GGATTGCCAG GTCCACCTGG ATTGCCAGGT CTTCCCGGAA AGGAAGGAAT TCCAGGTCCT 
CCTGGCCCCC CAGGACTCCC TGGAAAAGAC GGGTTGTCTG GTACACCTGG TGCTGCTGGA 
TTACGGGGTG AAGATGGAGA GATTGGTCTT CCAGGTGCTC CTGGAACAAA GGGTAGCACT 
GGTGAGCCAG GACTACCTGG ACCTCCTGGA CAATCTGGTT TAGCTGGACT TCCTGGACCA 
ATGGGACCAC GCGGGCTGCC AGGTCCTCCA GGACCACCTG GGCCTGTGGG TCATGGATCT 
GCAATTGGCT TTGATGACAT GGAAGCCTCA GGGCTTGGAT TTGGCTTGAG AGAAGGGACA 
CAGGGGGTGC CTGGACTTCC CGGGGCAAAG GGAGAGCCAG GACATCCAGG GCTGCCAGGA 
GTGAAGGGAG ATAGTGGTTT TCCAGGCCTT GAAGGAAGGC CGGGGTTGGA TGGATTCCCA 
GGTCCAGAGG GATTAAAGGG TGAGAAAGGT GAACGTGGTG AGCGGGGAGA ATCTGGCCGT 
GATGGTGTTG GTATTCCAGG TCCACCGGGA CCTCCTGGTC AGGTGCTTTA TTTACCATCC 
GATGACAAGA CTATTGTTGG TGCCATTCCT GGACCAGAGG GCAGGCAAGG TGTAGCTGGA 
CTTCCGGGCC CAAGGGGACC CAAAGGAGAG GCGGGCTTGA ACGGTATCCC AGGAACCTTT 
GGAGTAAAGG GAGAAAAGGG AGAGCCTGGA ATTATTATGG GACCAGATAG CACCATTTAC 
ACTGATCTAT CTAAAGGAGA GAAGGGTGAC GAAGGACCAA GGGGACCAGC AGGAGCTGCG 
GGACCATTTG GACAAAAGGG AGAAATCGGG TTCCCAGGAC GCCCTGGACG TCCTGGTATG 
AATGGACTGA AAGGGGAGAA AGGAGAACCT AGTGGTGCAA GTGGTGGCTT TGGACTGAGG 
GGGCCACCTG GTCCTCCTGG CCCACCCGGT CCTCCTGGCA GTGTGGTGTA TGGAGATCCA 
AATAACCCAG GAGCCCAAGT ATATCCAGGT CATAAAGGGG AGAGAGGATT TCCTGGACCC 
AAAGGTGATG TTGGTCCTGC TGGCCCACCA GGCTCTTTCC CATATGATTT AAGTACCTTT 
GCCTCGATTT TAAGGAGAGA AGCTGGACTC AAAGGAGAGA AGGGCGAATC TGGGGGTGGT 
TCCCATGGAC AGGGGATCCC AGGGCCACCT GGAAACCCTG GATATCCAGG ACTGCCGGGA 
GCTAAAGGAG ACAGTGTTCA AGGTCCTCCA GGCCCAGCCG GCCCACCAGG ACCTTCTGGA 
ATCGGTTATG AAGGAAGGCA AGGTCCACCA GGGCCCCCAG GTCCTCCAGG CCTGCCAGGT 
TCCTCCTCTT ATCCTGGGCC TCACAGGCAA ACCATCAGCA TACCTGGACC TCCTGGCCCA 
CCAGGCCCAC CAGGGCTCCC AGGAACATCA GGAGGATTAT CTGGGGCTAC TGTGCTACAG 
ACCTATCAGA CTATGATCAA CTTAGCCCAC AGGCTGCAGG AAGGGACAAT TGTGCTGGTC 
CAGGAGAGGT CGGAACTGTA TGTGCGTGTG AGAGAAGGCT TCCGAAAAAT TCTTCTTGGT 
GAATATACAA CATCCTCTGG CCCTGGACAG GATAATGAAG TTGCTGCGGA GCAGGCCCCT 
GTAGTTCCAT ATCCACACCG TAACAAAGAT TCTGATTACC TCCAGTCTAC AGAGGAGAAC 
AGCAACAATC CAAATGTGCC CTCAGGTGCC CGACCCTGGA GCGATCCTCG GTTACCAGAA 
CCACCAAGAG TAAACCCACC ACCACCCCCA GACCATCACC ACCATGGAAA CCCTCGTCCA 
TTGGAACCAT CCCCAGTGCA CACACACCAA GAGTTTAATC CTGTGCTTCA CCTTGTGGCC 
CTGAACACTC CACTGAGTGG CAGCATGAAA TCCATCCGCG GAGTGGACTT CCAGTGCTTT 
GAGCAAGCCC GTAAAGCTGG ACTACATGGC ACATTTAGAG CGTTTTTGTC TTCCCGACTT 
CAAGACCTGT ACAGCATTGT ACGCAGAGCT GATCGACAAT CTGTGCAAAT TGTTAATCTT 
AGGGATGAAG TGTTATTTGA CAACTGGGAG TCCCTGTTCT CTGGTTCTGA GGCTCAGATG 
AGGCCTGGTG CGAGTATCTT CTCTTTTGAT GGAAAAGATG TTGCAACAGA TCCTACATGG 
CCACAGAAGA TGGTTTGGCA TGGGTCTGAC TCGAAGGGAC GGCGGCTTAC TGAAAGCTAT 
TGCGAGACCT GGCGCACAGA TGAAGGTGCA GTAACTGGTC AGGCATCATC GCTGACGAGC 
GGGAAACTCT TAGAACAGCG CCCACAGAGT TGCAACAAGA ACTTCATTGT TTTGTGTATT 
GAGAACAGCT TCATGACCAA TAACCGGAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826360.2
CDS	177..4109
Translation	MILRCTWVLLLYASYHVLLVSSQGKDNFNPEVGLLQLIGEPVPEQITKVFGPDKTPAYVFGKEANTGQVAQYHLPNPMYRDFSLLFHVQPTTDKAGILFAITDASQSIVYIGVKLSEVKDGKQDIIFYYTEPGSQDSYVAATFNVPSLVNRWSRFAISVEEEEVVLFMDCDELERVRFERSPDEMELEDGSGLFVAQAGGADPDRYQGVIADLKIVADPRAAEHQCEEDGDDSDGISGDFGSGIADNDLVTSPPVIIKVQPPITVPPIQRTPVEKEIVQTGQGQQGVKGEKGDRGEKGERGLTGPKGDGGNASPTSDSKGEKGQKGEVGVKGSAGFGYPGPKGQKGDPGDLGPRGPPGPPGPSILQKSDGTTVQQVTGPPGPQGPAGPPGKEGEPGDPGEDGKTGEVGPQGFPGTPGDSGQKGDKGDSGVGIRGAPGPPGPPGPPGPSRTDKLTFIDMEGSGYGGDSEHIRGAPGVPGPPGPPGVPGLPGVPGTFGMNNTGLPGPPGLPGLPGKEGIPGPPGPPGLPGKDGLSGTPGAAGLRGEDGEIGLPGAPGTKGSTGEPGLPGPPGQSGLAGLPGPMGPRGLPGPPGPPGPVGHGSAIGFDDMEASGLGFGLREGTQGVPGLPGAKGEPGHPGLPGVKGDSGFPGLEGRPGLDGFPGPEGLKGEKGERGERGESGRDGVGIPGPPGPPGQVLYLPSDDKTIVGAIPGPEGRQGVAGLPGPRGPKGEAGLNGIPGTFGVKGEKGEPGIIMGPDSTIYTDLSKGEKGDEGPRGPAGAAGPFGQKGEIGFPGRPGRPGMNGLKGEKGEPSGASGGFGLRGPPGPPGPPGPPGSVVYGDPNNPGAQVYPGHKGERGFPGPKGDVGPAGPPGSFPYDLSTFASILRREAGLKGEKGESGGGSHGQGIPGPPGNPGYPGLPGAKGDSVQGPPGPAGPPGPSGIGYEGRQGPPGPPGPPGLPGSSSYPGPHRQTISIPGPPGPPGPPGLPGTSGGLSGATVLQTYQTMINLAHRLQEGTIVLVQERSELYVRVREGFRKILLGEYTTSSGPGQDNEVAAEQAPVVPYPHRNKDSDYLQSTEENSNNPNVPSGARPWSDPRLPEPPRVNPPPPPDHHHHGNPRPLEPSPVHTHQEFNPVLHLVALNTPLSGSMKSIRGVDFQCFEQARKAGLHGTFRAFLSSRLQDLYSIVRRADRQSVQIVNLRDEVLFDNWESLFSGSEAQMRPGASIFSFDGKDVATDPTWPQKMVWHGSDSKGRRLTESYCETWRTDEGAVTGQASSLTSGKLLEQRPQSCNKNFIVLCIENSFMTNNRK

Target ORF information:

RefSeq Version	XM_012970906.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen type XVIII alpha 1 (col18a1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970906.3

ORF Insert Sequence:

ATGATTCTCA GGTGTACGTG GGTCCTGTTG CTCTACGCAT CGTATCATGT TCTTCTGGTC 
TCCTCCCAAG GCAAAGACAA CTTCAACCCA GAAGTTGGCC TCTTGCAGTT GATTGGAGAA 
CCTGTACCTG AACAGATCAC CAAGGTCTTT GGGCCCGACA AGACTCCCGC CTATGTATTT 
GGTAAAGAAG CCAATACAGG CCAGGTGGCC CAATACCATC TGCCCAACCC TATGTATAGG 
GACTTCTCTC TCCTCTTCCA TGTCCAGCCA ACCACAGACA AGGCCGGCAT CCTCTTTGCC 
ATCACAGATG CCTCTCAGTC TATTGTGTAT ATCGGGGTGA AGCTTTCTGA AGTCAAGGAT 
GGGAAGCAAG ACATCATCTT CTACTATACA GAGCCTGGCT CACAGGATTC CTATGTAGCT 
GCCACCTTCA ATGTCCCATC ACTGGTGAAT CGTTGGTCTC GCTTTGCAAT CAGTGTGGAG 
GAAGAAGAAG TTGTGCTGTT TATGGATTGT GATGAGCTGG AAAGGGTGCG CTTCGAGAGA 
TCACCTGATG AGATGGAACT AGAAGATGGC TCTGGGCTCT TTGTTGCTCA AGCAGGTGGC 
GCTGATCCAG ACAGATATCA GGGTGTAATA GCAGATCTAA AGATCGTAGC CGATCCAAGA 
GCCGCAGAAC ATCAGTGTGA GGAGGATGGA GATGACTCTG ATGGGATCTC TGGAGATTTT 
GGCAGTGGCA TTGCGGACAA TGATTTGGTG ACTTCTCCTC CTGTGATAAT CAAAGTGCAG 
CCTCCTATAA CTGTCCCTCC CATACAGAGG ACCCCTGTGG AGAAAGAGAT TGTTCAGACA 
GGTCAAGGCC AGCAAGGGGT AAAAGGAGAG AAGGGTGATC GTGGAGAGAA GGGTGAGAGG 
GGACTGACTG GCCCTAAGGG AGACGGTGGG AATGCAAGTC CAACTTCTGA TTCCAAAGGA 
GAAAAGGGTC AGAAAGGAGA GGTCGGAGTG AAGGGAAGTG CAGGCTTTGG CTATCCCGGG 
CCTAAAGGGC AGAAAGGAGA TCCTGGAGAC TTGGGACCAA GAGGACCACC AGGCCCACCT 
GGACCATCTA TATTACAGAA AAGTGATGGA ACTACAGTGC AACAGGTCAC AGGACCTCCA 
GGTCCTCAAG GCCCTGCGGG ACCACCAGGA AAAGAAGGGG AACCAGGTGA CCCTGGTGAA 
GATGGAAAAA CTGGCGAGGT TGGTCCCCAA GGATTTCCAG GGACCCCAGG AGATTCTGGG 
CAGAAGGGAG ACAAGGGTGA TTCTGGTGTT GGTATCAGAG GTGCTCCTGG GCCACCAGGT 
CCGCCAGGCC CCCCTGGACC TTCTAGAACC GACAAGCTGA CATTCATTGA CATGGAAGGT 
TCTGGATATG GTGGAGATTC GGAGCACATC AGGGGAGCCC CTGGTGTGCC TGGGCCCCCA 
GGTCCTCCAG GTGTACCAGG GTTACCCGGA GTACCCGGAA CTTTTGGCAT GAACAACACA 
GGATTGCCAG GTCCACCTGG ATTGCCAGGT CTTCCCGGAA AGGAAGGAAT TCCAGGTCCT 
CCTGGCCCCC CAGGACTCCC TGGAAAAGAC GGGTTGTCTG GTACACCTGG TGCTGCTGGA 
TTACGGGGTG AAGATGGAGA GATTGGTCTT CCAGGTGCTC CTGGAACAAA GGGTAGCACT 
GGTGAGCCAG GACTACCTGG ACCTCCTGGA CAATCTGGTT TAGCTGGACT TCCTGGACCA 
ATGGGACCAC GCGGGCTGCC AGGTCCTCCA GGACCACCTG GGCCTGTGGG TCATGGATCT 
GCAATTGGCT TTGATGACAT GGAAGCCTCA GGGCTTGGAT TTGGCTTGAG AGAAGGGACA 
CAGGGGGTGC CTGGACTTCC CGGGGCAAAG GGAGAGCCAG GACATCCAGG GCTGCCAGGA 
GTGAAGGGAG ATAGTGGTTT TCCAGGCCTT GAAGGAAGGC CGGGGTTGGA TGGATTCCCA 
GGTCCAGAGG GATTAAAGGG TGAGAAAGGT GAACGTGGTG AGCGGGGAGA ATCTGGCCGT 
GATGGTGTTG GTATTCCAGG TCCACCGGGA CCTCCTGGTC AGGTGCTTTA TTTACCATCC 
GATGACAAGA CTATTGTTGG TGCCATTCCT GGACCAGAGG GCAGGCAAGG TGTAGCTGGA 
CTTCCGGGCC CAAGGGGACC CAAAGGAGAG GCGGGCTTGA ACGGTATCCC AGGAACCTTT 
GGAGTAAAGG GAGAAAAGGG AGAGCCTGGA ATTATTATGG GACCAGATAG CACCATTTAC 
ACTGATCTAT CTAAAGGAGA GAAGGGTGAC GAAGGACCAA GGGGACCAGC AGGAGCTGCG 
GGACCATTTG GACAAAAGGG AGAAATCGGG TTCCCAGGAC GCCCTGGACG TCCTGGTATG 
AATGGACTGA AAGGGGAGAA AGGAGAACCT AGTGGTGCAA GTGGTGGCTT TGGACTGAGG 
GGGCCACCTG GTCCTCCTGG CCCACCCGGT CCTCCTGGCA GTGTGGTGTA TGGAGATCCA 
AATAACCCAG GAGCCCAAGT ATATCCAGGT CATAAAGGGG AGAGAGGATT TCCTGGACCC 
AAAGGTGATG TTGGTCCTGC TGGCCCACCA GGCTCTTTCC CATATGATTT AAGTACCTTT 
GCCTCGATTT TAAGGAGAGA AGCTGGACTC AAAGGAGAGA AGGGCGAATC TGGGGGTGGT 
TCCCATGGAC AGGGGATCCC AGGGCCACCT GGAAACCCTG GATATCCAGG ACTGCCGGGA 
GCTAAAGGAG ACAGTGTTCA AGGTCCTCCA GGCCCAGCCG GCCCACCAGG ACCTTCTGGA 
ATCGGTTATG AAGGAAGGCA AGGTCCACCA GGGCCCCCAG GTCCTCCAGG CCTGCCAGGT 
TCCTCCTCTT ATCCTGGGCC TCACAGGCAA ACCATCAGCA TACCTGGACC TCCTGGCCCA 
CCAGGCCCAC CAGGGCTCCC AGGAACATCA GGAGGATTAT CTGGGGCTAC TGTGCTACAG 
ACCTATCAGA CTATGATCAA CTTAGCCCAC AGGCTGCAGG AAGGGACAAT TGTGCTGGTC 
CAGGAGAGGT CGGAACTGTA TGTGCGTGTG AGAGAAGGCT TCCGAAAAAT TCTTCTTGGT 
GAATATACAA CATCCTCTGG CCCTGGACAG GATAATGAAG TTGCTGCGGA GCAGGCCCCT 
GTAGTTCCAT ATCCACACCG TAACAAAGAT TCTGATTACC TCCAGTCTAC AGAGGAGAAC 
AGCAACAATC CAAATGTGCC CTCAGGTGCC CGACCCTGGA GCGATCCTCG GTTACCAGAA 
CCACCAAGAG TAAACCCACC ACCACCCCCA GACCATCACC ACCATGGAAA CCCTCGTCCA 
TTGGAACCAT CCCCAGTGCA CACACACCAA GAGTTTAATC CTGTGCTTCA CCTTGTGGCC 
CTGAACACTC CACTGAGTGG CAGCATGAAA TCCATCCGCG GAGTGGACTT CCAGTGCTTT 
GAGCAAGCCC GTAAAGCTGG ACTACATGGC ACATTTAGAG CGTTTTTGTC TTCCCGACTT 
CAAGACCTGT ACAGCATTGT ACGCAGAGCT GATCGACAAT CTGTGCAAAT TGTTAATCTT 
AGGGATGAAG TGTTATTTGA CAACTGGGAG TCCCTGTTCT CTGGTTCTGA GGCTCAGATG 
AGGCCTGGTG CGAGTATCTT CTCTTTTGAT GGAAAAGATG TTGCAACAGA TCCTACATGG 
CCACAGAAGA TGGTTTGGCA TGGGTCTGAC TCGAAGGGAC GGCGGCTTAC TGAAAGCTAT 
TGCGAGACCT GGCGCACAGA TGAAGGTGCA GTAACTGGTC AGGCATCATC GCTGACGAGC 
GGGAAACTCT TAGAACAGCG CCCACAGAGT TGCAACAAGA ACTTCATTGT TTTGTGTATT 
GAGAACAGCT TCATGACCAA TAACCGGAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col18a1 ( XM_012970907.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826361.2 Xenopus tropicalis collagen type XVIII alpha 1 (col18a1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa66573
Clone ID Related Accession (Same CDS sequence)	XM_012970907.3
Accession Version	XM_012970907.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(XVIII) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970907.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCTCA GGTGTACGTG GGTCCTGTTG CTCTACGCAT CGTATCATGT TCTTCTGGTC 
TCCTCCCAAG GCAAAGACAA CTTCAACCCA GAAGTTGGCC TCTTGCAGTT GATTGGAGAA 
CCTGTACCTG AACAGATCAC CAAGGTCTTT GGGCCCGACA AGACTCCCGC CTATGTATTT 
GGTAAAGAAG CCAATACAGG CCAGGTGGCC CAATACCATC TGCCCAACCC TATGTATAGG 
GACTTCTCTC TCCTCTTCCA TGTCCAGCCA ACCACAGACA AGGCCGGCAT CCTCTTTGCC 
ATCACAGATG CCTCTCAGTC TATTGTGTAT ATCGGGGTGA AGCTTTCTGA AGTCAAGGAT 
GGGAAGCAAG ACATCATCTT CTACTATACA GAGCCTGGCT CACAGGATTC CTATGTAGCT 
GCCACCTTCA ATGTCCCATC ACTGGTGAAT CGTTGGTCTC GCTTTGCAAT CAGTGTGGAG 
GAAGAAGAAG TTGTGCTGTT TATGGATTGT GATGAGCTGG AAAGGGTGCG CTTCGAGAGA 
TCACCTGATG AGATGGAACT AGAAGATGGC TCTGGGCTCT TTGTTGCTCA AGCAGGTGGC 
GCTGATCCAG ACAGATATCA GGGTGTAATA GCAGATCTAA AGATCGTAGC CGATCCAAGA 
GCCGCAGAAC ATCAGTGTGA GGAGGATGGA GATGACTCTG ATGGGATCTC TGGAGATTTT 
GGCAGTGGCA TTGCGGACAA TGATTTGGTG ACTTCTCCTC CTGTGATAAT CAAAGTGCAG 
CCTCCTATAA CTGTCCCTCC CATACAGAGG ACCCCTGTGG AGAAAGAGAT TGTTCAGACA 
GGTCAAGGCC AGCAAGGGGT AAAAGGAGAG AAGGGTGATC GTGGAGAGAA GGGTGAGAGG 
GGACTGACTG GCCCTAAGGG AGACGGTGGG AATGCAAGTC CAACTTCTGA TTCCAAAGGA 
GAAAAGGGTC AGAAAGGAGA GGTCGGAGTG AAGGGAAGTG CAGGCTTTGG CTATCCCGGG 
CCTAAAGGGC AGAAAGGAGA TCCTGGAGAC TTGGGACCAA GAGGACCACC AGGCCCACCT 
GGACCATCTA TATTACAGAA AAGTGATGGA ACTACAGTGC AACAGGTCAC AGGACCTCCA 
GGTCCTCAAG GCCCTGCGGG ACCACCAGGA AAAGAAGGGG AACCAGGTGA CCCTGGTGAA 
GATGGAAAAA CTGGCGAGGT TGGTCCCCAA GGATTTCCAG GGACCCCAGG AGATTCTGGG 
CAGAAGGGAG ACAAGGGTGA TTCTGGTGTT GGTATCAGAG GTGCTCCTGG GCCACCAGGT 
CCGCCAGGCC CCCCTGGACC TTCTAGAACC GACAAGCTGA CATTCATTGA CATGGAAGGT 
TCTGGATATG GTGGAGATTC GGAGCACATC AGGGGAGCCC CTGGTGTGCC TGGGCCCCCA 
GGTCCTCCAG GTGTACCAGG GTTACCCGGA GTACCCGGAA CTTTTGGCAT GAACAACACA 
GGATTGCCAG GTCCACCTGG ATTGCCAGGT CTTCCCGGAA AGGAAGGAAT TCCAGGTCCT 
CCTGGCCCCC CAGGACTCCC TGGAAAAGAC GGGTTGTCTG GTACACCTGG TGCTGCTGGA 
TTACGGGGTG AAGATGGAGA GATTGGTCTT CCAGGTGCTC CTGGAACAAA GGGTAGCACT 
GGTGAGCCAG GACTACCTGG ACCTCCTGGA CAATCTGGTT TAGCTGGACT TCCTGGACCA 
ATGGGACCAC GCGGGCTGCC AGGTCCTCCA GGACCACCTG GGCCTGTGGG TCATGGATCT 
GCAATTGGCT TTGATGACAT GGAAGCCTCA GGGCTTGGAT TTGGCTTGAG AGAAGGGACA 
CAGGGGGTGC CTGGACTTCC CGGGGCAAAG GGAGAGCCAG GACATCCAGG GCTGCCAGGA 
GTGAAGGGAG ATAGTGGTTT TCCAGGCCTT GAAGGAAGGC CGGGGTTGGA TGGATTCCCA 
GGTCCAGAGG GATTAAAGGG TGAGAAAGGT GAACGTGGTG AGCGGGGAGA ATCTGGCCGT 
GATGGTGTTG GTATTCCAGG TCCACCGGGA CCTCCTGGTC AGGTGCTTTA TTTACCATCC 
GATGACAAGA CTATTGTTGG TGCCATTCCT GGACCAGAGG GCCCAAGGGG ACCCAAAGGA 
GAGGCGGGCT TGAACGGTAT CCCAGGAACC TTTGGAGTAA AGGGAGAAAA GGGAGAGCCT 
GGAATTATTA TGGGACCAGA TAGCACCATT TACACTGATC TATCTAAAGG AGAGAAGGGT 
GACGAAGGAC CAAGGGGACC AGCAGGAGCT GCGGGACCAT TTGGACAAAA GGGAGAAATC 
GGGTTCCCAG GACGCCCTGG ACGTCCTGGT ATGAATGGAC TGAAAGGGGA GAAAGGAGAA 
CCTAGTGGTG CAAGTGGTGG CTTTGGACTG AGGGGGCCAC CTGGTCCTCC TGGCCCACCC 
GGTCCTCCTG GCAGTGTGGT GTATGGAGAT CCAAATAACC CAGGAGCCCA AGTATATCCA 
GGTCATAAAG GGGAGAGAGG ATTTCCTGGA CCCAAAGGTG ATGTTGGTCC TGCTGGCCCA 
CCAGGCTCTT TCCCATATGA TTTAAGTACC TTTGCCTCGA TTTTAAGGAG AGAAGCTGGA 
CTCAAAGGAG AGAAGGGCGA ATCTGGGGGT GGTTCCCATG GACAGGGGAT CCCAGGGCCA 
CCTGGAAACC CTGGATATCC AGGACTGCCG GGAGCTAAAG GAGACAGTGT TCAAGGTCCT 
CCAGGCCCAG CCGGCCCACC AGGACCTTCT GGAATCGGTT ATGAAGGAAG GCAAGGTCCA 
CCAGGGCCCC CAGGTCCTCC AGGCCTGCCA GGTTCCTCCT CTTATCCTGG GCCTCACAGG 
CAAACCATCA GCATACCTGG ACCTCCTGGC CCACCAGGCC CACCAGGGCT CCCAGGAACA 
TCAGGAGGAT TATCTGGGGC TACTGTGCTA CAGACCTATC AGACTATGAT CAACTTAGCC 
CACAGGCTGC AGGAAGGGAC AATTGTGCTG GTCCAGGAGA GGTCGGAACT GTATGTGCGT 
GTGAGAGAAG GCTTCCGAAA AATTCTTCTT GGTGAATATA CAACATCCTC TGGCCCTGGA 
CAGGATAATG AAGTTGCTGC GGAGCAGGCC CCTGTAGTTC CATATCCACA CCGTAACAAA 
GATTCTGATT ACCTCCAGTC TACAGAGGAG AACAGCAACA ATCCAAATGT GCCCTCAGGT 
GCCCGACCCT GGAGCGATCC TCGGTTACCA GAACCACCAA GAGTAAACCC ACCACCACCC 
CCAGACCATC ACCACCATGG AAACCCTCGT CCATTGGAAC CATCCCCAGT GCACACACAC 
CAAGAGTTTA ATCCTGTGCT TCACCTTGTG GCCCTGAACA CTCCACTGAG TGGCAGCATG 
AAATCCATCC GCGGAGTGGA CTTCCAGTGC TTTGAGCAAG CCCGTAAAGC TGGACTACAT 
GGCACATTTA GAGCGTTTTT GTCTTCCCGA CTTCAAGACC TGTACAGCAT TGTACGCAGA 
GCTGATCGAC AATCTGTGCA AATTGTTAAT CTTAGGGATG AAGTGTTATT TGACAACTGG 
GAGTCCCTGT TCTCTGGTTC TGAGGCTCAG ATGAGGCCTG GTGCGAGTAT CTTCTCTTTT 
GATGGAAAAG ATGTTGCAAC AGATCCTACA TGGCCACAGA AGATGGTTTG GCATGGGTCT 
GACTCGAAGG GACGGCGGCT TACTGAAAGC TATTGCGAGA CCTGGCGCAC AGATGAAGGT 
GCAGTAACTG GTCAGGCATC ATCGCTGACG AGCGGGAAAC TCTTAGAACA GCGCCCACAG 
AGTTGCAACA AGAACTTCAT TGTTTTGTGT ATTGAGAACA GCTTCATGAC CAATAACCGG 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826361.2
CDS	177..4082
Translation	MILRCTWVLLLYASYHVLLVSSQGKDNFNPEVGLLQLIGEPVPEQITKVFGPDKTPAYVFGKEANTGQVAQYHLPNPMYRDFSLLFHVQPTTDKAGILFAITDASQSIVYIGVKLSEVKDGKQDIIFYYTEPGSQDSYVAATFNVPSLVNRWSRFAISVEEEEVVLFMDCDELERVRFERSPDEMELEDGSGLFVAQAGGADPDRYQGVIADLKIVADPRAAEHQCEEDGDDSDGISGDFGSGIADNDLVTSPPVIIKVQPPITVPPIQRTPVEKEIVQTGQGQQGVKGEKGDRGEKGERGLTGPKGDGGNASPTSDSKGEKGQKGEVGVKGSAGFGYPGPKGQKGDPGDLGPRGPPGPPGPSILQKSDGTTVQQVTGPPGPQGPAGPPGKEGEPGDPGEDGKTGEVGPQGFPGTPGDSGQKGDKGDSGVGIRGAPGPPGPPGPPGPSRTDKLTFIDMEGSGYGGDSEHIRGAPGVPGPPGPPGVPGLPGVPGTFGMNNTGLPGPPGLPGLPGKEGIPGPPGPPGLPGKDGLSGTPGAAGLRGEDGEIGLPGAPGTKGSTGEPGLPGPPGQSGLAGLPGPMGPRGLPGPPGPPGPVGHGSAIGFDDMEASGLGFGLREGTQGVPGLPGAKGEPGHPGLPGVKGDSGFPGLEGRPGLDGFPGPEGLKGEKGERGERGESGRDGVGIPGPPGPPGQVLYLPSDDKTIVGAIPGPEGPRGPKGEAGLNGIPGTFGVKGEKGEPGIIMGPDSTIYTDLSKGEKGDEGPRGPAGAAGPFGQKGEIGFPGRPGRPGMNGLKGEKGEPSGASGGFGLRGPPGPPGPPGPPGSVVYGDPNNPGAQVYPGHKGERGFPGPKGDVGPAGPPGSFPYDLSTFASILRREAGLKGEKGESGGGSHGQGIPGPPGNPGYPGLPGAKGDSVQGPPGPAGPPGPSGIGYEGRQGPPGPPGPPGLPGSSSYPGPHRQTISIPGPPGPPGPPGLPGTSGGLSGATVLQTYQTMINLAHRLQEGTIVLVQERSELYVRVREGFRKILLGEYTTSSGPGQDNEVAAEQAPVVPYPHRNKDSDYLQSTEENSNNPNVPSGARPWSDPRLPEPPRVNPPPPPDHHHHGNPRPLEPSPVHTHQEFNPVLHLVALNTPLSGSMKSIRGVDFQCFEQARKAGLHGTFRAFLSSRLQDLYSIVRRADRQSVQIVNLRDEVLFDNWESLFSGSEAQMRPGASIFSFDGKDVATDPTWPQKMVWHGSDSKGRRLTESYCETWRTDEGAVTGQASSLTSGKLLEQRPQSCNKNFIVLCIENSFMTNNRK

Target ORF information:

RefSeq Version	XM_012970907.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen type XVIII alpha 1 (col18a1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970907.3

ORF Insert Sequence:

ATGATTCTCA GGTGTACGTG GGTCCTGTTG CTCTACGCAT CGTATCATGT TCTTCTGGTC 
TCCTCCCAAG GCAAAGACAA CTTCAACCCA GAAGTTGGCC TCTTGCAGTT GATTGGAGAA 
CCTGTACCTG AACAGATCAC CAAGGTCTTT GGGCCCGACA AGACTCCCGC CTATGTATTT 
GGTAAAGAAG CCAATACAGG CCAGGTGGCC CAATACCATC TGCCCAACCC TATGTATAGG 
GACTTCTCTC TCCTCTTCCA TGTCCAGCCA ACCACAGACA AGGCCGGCAT CCTCTTTGCC 
ATCACAGATG CCTCTCAGTC TATTGTGTAT ATCGGGGTGA AGCTTTCTGA AGTCAAGGAT 
GGGAAGCAAG ACATCATCTT CTACTATACA GAGCCTGGCT CACAGGATTC CTATGTAGCT 
GCCACCTTCA ATGTCCCATC ACTGGTGAAT CGTTGGTCTC GCTTTGCAAT CAGTGTGGAG 
GAAGAAGAAG TTGTGCTGTT TATGGATTGT GATGAGCTGG AAAGGGTGCG CTTCGAGAGA 
TCACCTGATG AGATGGAACT AGAAGATGGC TCTGGGCTCT TTGTTGCTCA AGCAGGTGGC 
GCTGATCCAG ACAGATATCA GGGTGTAATA GCAGATCTAA AGATCGTAGC CGATCCAAGA 
GCCGCAGAAC ATCAGTGTGA GGAGGATGGA GATGACTCTG ATGGGATCTC TGGAGATTTT 
GGCAGTGGCA TTGCGGACAA TGATTTGGTG ACTTCTCCTC CTGTGATAAT CAAAGTGCAG 
CCTCCTATAA CTGTCCCTCC CATACAGAGG ACCCCTGTGG AGAAAGAGAT TGTTCAGACA 
GGTCAAGGCC AGCAAGGGGT AAAAGGAGAG AAGGGTGATC GTGGAGAGAA GGGTGAGAGG 
GGACTGACTG GCCCTAAGGG AGACGGTGGG AATGCAAGTC CAACTTCTGA TTCCAAAGGA 
GAAAAGGGTC AGAAAGGAGA GGTCGGAGTG AAGGGAAGTG CAGGCTTTGG CTATCCCGGG 
CCTAAAGGGC AGAAAGGAGA TCCTGGAGAC TTGGGACCAA GAGGACCACC AGGCCCACCT 
GGACCATCTA TATTACAGAA AAGTGATGGA ACTACAGTGC AACAGGTCAC AGGACCTCCA 
GGTCCTCAAG GCCCTGCGGG ACCACCAGGA AAAGAAGGGG AACCAGGTGA CCCTGGTGAA 
GATGGAAAAA CTGGCGAGGT TGGTCCCCAA GGATTTCCAG GGACCCCAGG AGATTCTGGG 
CAGAAGGGAG ACAAGGGTGA TTCTGGTGTT GGTATCAGAG GTGCTCCTGG GCCACCAGGT 
CCGCCAGGCC CCCCTGGACC TTCTAGAACC GACAAGCTGA CATTCATTGA CATGGAAGGT 
TCTGGATATG GTGGAGATTC GGAGCACATC AGGGGAGCCC CTGGTGTGCC TGGGCCCCCA 
GGTCCTCCAG GTGTACCAGG GTTACCCGGA GTACCCGGAA CTTTTGGCAT GAACAACACA 
GGATTGCCAG GTCCACCTGG ATTGCCAGGT CTTCCCGGAA AGGAAGGAAT TCCAGGTCCT 
CCTGGCCCCC CAGGACTCCC TGGAAAAGAC GGGTTGTCTG GTACACCTGG TGCTGCTGGA 
TTACGGGGTG AAGATGGAGA GATTGGTCTT CCAGGTGCTC CTGGAACAAA GGGTAGCACT 
GGTGAGCCAG GACTACCTGG ACCTCCTGGA CAATCTGGTT TAGCTGGACT TCCTGGACCA 
ATGGGACCAC GCGGGCTGCC AGGTCCTCCA GGACCACCTG GGCCTGTGGG TCATGGATCT 
GCAATTGGCT TTGATGACAT GGAAGCCTCA GGGCTTGGAT TTGGCTTGAG AGAAGGGACA 
CAGGGGGTGC CTGGACTTCC CGGGGCAAAG GGAGAGCCAG GACATCCAGG GCTGCCAGGA 
GTGAAGGGAG ATAGTGGTTT TCCAGGCCTT GAAGGAAGGC CGGGGTTGGA TGGATTCCCA 
GGTCCAGAGG GATTAAAGGG TGAGAAAGGT GAACGTGGTG AGCGGGGAGA ATCTGGCCGT 
GATGGTGTTG GTATTCCAGG TCCACCGGGA CCTCCTGGTC AGGTGCTTTA TTTACCATCC 
GATGACAAGA CTATTGTTGG TGCCATTCCT GGACCAGAGG GCCCAAGGGG ACCCAAAGGA 
GAGGCGGGCT TGAACGGTAT CCCAGGAACC TTTGGAGTAA AGGGAGAAAA GGGAGAGCCT 
GGAATTATTA TGGGACCAGA TAGCACCATT TACACTGATC TATCTAAAGG AGAGAAGGGT 
GACGAAGGAC CAAGGGGACC AGCAGGAGCT GCGGGACCAT TTGGACAAAA GGGAGAAATC 
GGGTTCCCAG GACGCCCTGG ACGTCCTGGT ATGAATGGAC TGAAAGGGGA GAAAGGAGAA 
CCTAGTGGTG CAAGTGGTGG CTTTGGACTG AGGGGGCCAC CTGGTCCTCC TGGCCCACCC 
GGTCCTCCTG GCAGTGTGGT GTATGGAGAT CCAAATAACC CAGGAGCCCA AGTATATCCA 
GGTCATAAAG GGGAGAGAGG ATTTCCTGGA CCCAAAGGTG ATGTTGGTCC TGCTGGCCCA 
CCAGGCTCTT TCCCATATGA TTTAAGTACC TTTGCCTCGA TTTTAAGGAG AGAAGCTGGA 
CTCAAAGGAG AGAAGGGCGA ATCTGGGGGT GGTTCCCATG GACAGGGGAT CCCAGGGCCA 
CCTGGAAACC CTGGATATCC AGGACTGCCG GGAGCTAAAG GAGACAGTGT TCAAGGTCCT 
CCAGGCCCAG CCGGCCCACC AGGACCTTCT GGAATCGGTT ATGAAGGAAG GCAAGGTCCA 
CCAGGGCCCC CAGGTCCTCC AGGCCTGCCA GGTTCCTCCT CTTATCCTGG GCCTCACAGG 
CAAACCATCA GCATACCTGG ACCTCCTGGC CCACCAGGCC CACCAGGGCT CCCAGGAACA 
TCAGGAGGAT TATCTGGGGC TACTGTGCTA CAGACCTATC AGACTATGAT CAACTTAGCC 
CACAGGCTGC AGGAAGGGAC AATTGTGCTG GTCCAGGAGA GGTCGGAACT GTATGTGCGT 
GTGAGAGAAG GCTTCCGAAA AATTCTTCTT GGTGAATATA CAACATCCTC TGGCCCTGGA 
CAGGATAATG AAGTTGCTGC GGAGCAGGCC CCTGTAGTTC CATATCCACA CCGTAACAAA 
GATTCTGATT ACCTCCAGTC TACAGAGGAG AACAGCAACA ATCCAAATGT GCCCTCAGGT 
GCCCGACCCT GGAGCGATCC TCGGTTACCA GAACCACCAA GAGTAAACCC ACCACCACCC 
CCAGACCATC ACCACCATGG AAACCCTCGT CCATTGGAAC CATCCCCAGT GCACACACAC 
CAAGAGTTTA ATCCTGTGCT TCACCTTGTG GCCCTGAACA CTCCACTGAG TGGCAGCATG 
AAATCCATCC GCGGAGTGGA CTTCCAGTGC TTTGAGCAAG CCCGTAAAGC TGGACTACAT 
GGCACATTTA GAGCGTTTTT GTCTTCCCGA CTTCAAGACC TGTACAGCAT TGTACGCAGA 
GCTGATCGAC AATCTGTGCA AATTGTTAAT CTTAGGGATG AAGTGTTATT TGACAACTGG 
GAGTCCCTGT TCTCTGGTTC TGAGGCTCAG ATGAGGCCTG GTGCGAGTAT CTTCTCTTTT 
GATGGAAAAG ATGTTGCAAC AGATCCTACA TGGCCACAGA AGATGGTTTG GCATGGGTCT 
GACTCGAAGG GACGGCGGCT TACTGAAAGC TATTGCGAGA CCTGGCGCAC AGATGAAGGT 
GCAGTAACTG GTCAGGCATC ATCGCTGACG AGCGGGAAAC TCTTAGAACA GCGCCCACAG 
AGTTGCAACA AGAACTTCAT TGTTTTGTGT ATTGAGAACA GCTTCATGAC CAATAACCGG 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col18a1 ( XM_012970903.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826357.2 Xenopus tropicalis collagen type XVIII alpha 1 (col18a1), transcript variant X1, mRNA.

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CloneID	OXa66569
Clone ID Related Accession (Same CDS sequence)	XM_012970903.3
Accession Version	XM_012970903.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(XVIII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970903.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCAG CGCGGCTTAG CCTTGGGGTC ACAGTGCTTG TGCTCCTCTT ACCTGCTCTA 
TCCAATGGCC AGTTTTGGTC CTGGTTTACA GGGAGCAAAG AGACAACTGC GAGCCCCACA 
ACTCAACGTG TAACTCCAGG AACATCCTCA GTTCATAGTA CTGCTGTGCA GCATCACTCA 
CCTGATGCCT CGCCAACCAC ATTGCACCAT ATACCGGATG CACTACCCTC AGCGGAAAGT 
ACGTCAGCAC CTGCAGTGCA TCAGCCACCT GAAGAAGATA ACCTGCCTGA GACAACACCC 
TCTCACCTAG AGGCAGCCTC TGTGCCATCT TCAGAAAAAC CCACACCTTT TACAGAACAG 
TCACCCACAG CATCCTTCTC AGCCATACTC ATTCCCAGCA CAGTCATCCT AACCACAGAG 
GGCAAGAATG ACAGTACTGA GACAGCAGGA GTGACCAGAC CCCTCCAAAC GACAGAGTCC 
CCTACTACTT CCAGGAGGCA GGAGGAAAAG CAAATATGGA ACATATTCAG TGTGCAGAAA 
GGAAAGGAGA ACGTAGCGCC TGGGACTTTG GCCACAACAC CTGTAGCGAA GGATGAAACC 
AGTGCACCTT CTGTGTCAGA GGTTCCAATA TCTCAGAAGC CACAAAGTGA ACAAAACATT 
GCTGGTGTAG GGGCCGAGAT CTTAAATGTA GCAGAAGGAA TTAGGAACTT TGTGCATGGC 
TGGGAAGATA ACATTGCCAA CAGCACAAAG CAGACTCCTA CTCCTGACAC GGAGATGGTG 
ACTGTCACAA CAGCCCCAAG CCTTACTGTA AATGACACTG GCACCTTAAG CTACAACAAG 
ACAGAACCAA GCGAGAACAC TATGGCAAAC CATACGGAAG GTCCGGTGTC CGAGCCCCAT 
AAACTGATCT CATCCCACAA CAAGCCACTT CTACCAGACA ACTTCAACCC AGAAGTTGGC 
CTCTTGCAGT TGATTGGAGA ACCTGTACCT GAACAGATCA CCAAGGTCTT TGGGCCCGAC 
AAGACTCCCG CCTATGTATT TGGTAAAGAA GCCAATACAG GCCAGGTGGC CCAATACCAT 
CTGCCCAACC CTATGTATAG GGACTTCTCT CTCCTCTTCC ATGTCCAGCC AACCACAGAC 
AAGGCCGGCA TCCTCTTTGC CATCACAGAT GCCTCTCAGT CTATTGTGTA TATCGGGGTG 
AAGCTTTCTG AAGTCAAGGA TGGGAAGCAA GACATCATCT TCTACTATAC AGAGCCTGGC 
TCACAGGATT CCTATGTAGC TGCCACCTTC AATGTCCCAT CACTGGTGAA TCGTTGGTCT 
CGCTTTGCAA TCAGTGTGGA GGAAGAAGAA GTTGTGCTGT TTATGGATTG TGATGAGCTG 
GAAAGGGTGC GCTTCGAGAG ATCACCTGAT GAGATGGAAC TAGAAGATGG CTCTGGGCTC 
TTTGTTGCTC AAGCAGGTGG CGCTGATCCA GACAGATATC AGGGTGTAAT AGCAGATCTA 
AAGATCGTAG CCGATCCAAG AGCCGCAGAA CATCAGTGTG AGGAGGATGG AGATGACTCT 
GATGGGATCT CTGGAGATTT TGGCAGTGGC ATTGCGGACA ATGATTTGGT GACTTCTCCT 
CCTGTGATAA TCAAAGTGCA GCCTCCTATA ACTGTCCCTC CCATACAGAG GACCCCTGTG 
GAGAAAGAGA TTGTTCAGAC AGGTCAAGGC CAGCAAGGGG TAAAAGGAGA GAAGGGTGAT 
CGTGGAGAGA AGGGTGAGAG GGGACTGACT GGCCCTAAGG GAGACGGTGG GAATGCAAGT 
CCAACTTCTG ATTCCAAAGG AGAAAAGGGT CAGAAAGGAG AGGTCGGAGT GAAGGGAAGT 
GCAGGCTTTG GCTATCCCGG GCCTAAAGGG CAGAAAGGAG ATCCTGGAGA CTTGGGACCA 
AGAGGACCAC CAGGCCCACC TGGACCATCT ATATTACAGA AAAGTGATGG AACTACAGTG 
CAACAGGTCA CAGGACCTCC AGGTCCTCAA GGCCCTGCGG GACCACCAGG AAAAGAAGGG 
GAACCAGGTG ACCCTGGTGA AGATGGAAAA ACTGGCGAGG TTGGTCCCCA AGGATTTCCA 
GGGACCCCAG GAGATTCTGG GCAGAAGGGA GACAAGGGTG ATTCTGGTGT TGGTATCAGA 
GGTGCTCCTG GGCCACCAGG TCCGCCAGGC CCCCCTGGAC CTTCTAGAAC CGACAAGCTG 
ACATTCATTG ACATGGAAGG TTCTGGATAT GGTGGAGATT CGGAGCACAT CAGGGGAGCC 
CCTGGTGTGC CTGGGCCCCC AGGTCCTCCA GGTGTACCAG GGTTACCCGG AGTACCCGGA 
ACTTTTGGCA TGAACAACAC AGGATTGCCA GGTCCACCTG GATTGCCAGG TCTTCCCGGA 
AAGGAAGGAA TTCCAGGTCC TCCTGGCCCC CCAGGACTCC CTGGAAAAGA CGGGTTGTCT 
GGTACACCTG GTGCTGCTGG ATTACGGGGT GAAGATGGAG AGATTGGTCT TCCAGGTGCT 
CCTGGAACAA AGGGTAGCAC TGGTGAGCCA GGACTACCTG GACCTCCTGG ACAATCTGGT 
TTAGCTGGAC TTCCTGGACC AATGGGACCA CGCGGGCTGC CAGGTCCTCC AGGACCACCT 
GGGCCTGTGG GTCATGGATC TGCAATTGGC TTTGATGACA TGGAAGCCTC AGGGCTTGGA 
TTTGGCTTGA GAGAAGGGAC ACAGGGGGTG CCTGGACTTC CCGGGGCAAA GGGAGAGCCA 
GGACATCCAG GGCTGCCAGG AGTGAAGGGA GATAGTGGTT TTCCAGGCCT TGAAGGAAGG 
CCGGGGTTGG ATGGATTCCC AGGTCCAGAG GGATTAAAGG GTGAGAAAGG TGAACGTGGT 
GAGCGGGGAG AATCTGGCCG TGATGGTGTT GGTATTCCAG GTCCACCGGG ACCTCCTGGT 
CAGGTGCTTT ATTTACCATC CGATGACAAG ACTATTGTTG GTGCCATTCC TGGACCAGAG 
GGCAGGCAAG GTGTAGCTGG ACTTCCGGGC CCAAGGGGAC CCAAAGGAGA GGCGGGCTTG 
AACGGTATCC CAGGAACCTT TGGAGTAAAG GGAGAAAAGG GAGAGCCTGG AATTATTATG 
GGACCAGATA GCACCATTTA CACTGATCTA TCTAAAGGAG AGAAGGGTGA CGAAGGACCA 
AGGGGACCAG CAGGAGCTGC GGGACCATTT GGACAAAAGG GAGAAATCGG GTTCCCAGGA 
CGCCCTGGAC GTCCTGGTAT GAATGGACTG AAAGGGGAGA AAGGAGAACC TAGTGGTGCA 
AGTGGTGGCT TTGGACTGAG GGGGCCACCT GGTCCTCCTG GCCCACCCGG TCCTCCTGGC 
AGTGTGGTGT ATGGAGATCC AAATAACCCA GGAGCCCAAG TATATCCAGG TCATAAAGGG 
GAGAGAGGAT TTCCTGGACC CAAAGGTGAT GTTGGTCCTG CTGGCCCACC AGGCTCTTTC 
CCATATGATT TAAGTACCTT TGCCTCGATT TTAAGGAGAG AAGCTGGACT CAAAGGAGAG 
AAGGGCGAAT CTGGGGGTGG TTCCCATGGA CAGGGGATCC CAGGGCCACC TGGAAACCCT 
GGATATCCAG GACTGCCGGG AGCTAAAGGA GACAGTGTTC AAGGTCCTCC AGGCCCAGCC 
GGCCCACCAG GACCTTCTGG AATCGGTTAT GAAGGAAGGC AAGGTCCACC AGGGCCCCCA 
GGTCCTCCAG GCCTGCCAGG TTCCTCCTCT TATCCTGGGC CTCACAGGCA AACCATCAGC 
ATACCTGGAC CTCCTGGCCC ACCAGGCCCA CCAGGGCTCC CAGGAACATC AGGAGGATTA 
TCTGGGGCTA CTGTGCTACA GACCTATCAG ACTATGATCA ACTTAGCCCA CAGGCTGCAG 
GAAGGGACAA TTGTGCTGGT CCAGGAGAGG TCGGAACTGT ATGTGCGTGT GAGAGAAGGC 
TTCCGAAAAA TTCTTCTTGG TGAATATACA ACATCCTCTG GCCCTGGACA GGATAATGAA 
GTTGCTGCGG AGCAGGCCCC TGTAGTTCCA TATCCACACC GTAACAAAGA TTCTGATTAC 
CTCCAGTCTA CAGAGGAGAA CAGCAACAAT CCAAATGTGC CCTCAGGTGC CCGACCCTGG 
AGCGATCCTC GGTTACCAGA ACCACCAAGA GTAAACCCAC CACCACCCCC AGACCATCAC 
CACCATGGAA ACCCTCGTCC ATTGGAACCA TCCCCAGTGC ACACACACCA AGAGTTTAAT 
CCTGTGCTTC ACCTTGTGGC CCTGAACACT CCACTGAGTG GCAGCATGAA ATCCATCCGC 
GGAGTGGACT TCCAGTGCTT TGAGCAAGCC CGTAAAGCTG GACTACATGG CACATTTAGA 
GCGTTTTTGT CTTCCCGACT TCAAGACCTG TACAGCATTG TACGCAGAGC TGATCGACAA 
TCTGTGCAAA TTGTTAATCT TAGGGATGAA GTGTTATTTG ACAACTGGGA GTCCCTGTTC 
TCTGGTTCTG AGGCTCAGAT GAGGCCTGGT GCGAGTATCT TCTCTTTTGA TGGAAAAGAT 
GTTGCAACAG ATCCTACATG GCCACAGAAG ATGGTTTGGC ATGGGTCTGA CTCGAAGGGA 
CGGCGGCTTA CTGAAAGCTA TTGCGAGACC TGGCGCACAG ATGAAGGTGC AGTAACTGGT 
CAGGCATCAT CGCTGACGAG CGGGAAACTC TTAGAACAGC GCCCACAGAG TTGCAACAAG 
AACTTCATTG TTTTGTGTAT TGAGAACAGC TTCATGACCA ATAACCGGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826357.2
CDS	56..4849
Translation	MAPARLSLGVTVLVLLLPALSNGQFWSWFTGSKETTASPTTQRVTPGTSSVHSTAVQHHSPDASPTTLHHIPDALPSAESTSAPAVHQPPEEDNLPETTPSHLEAASVPSSEKPTPFTEQSPTASFSAILIPSTVILTTEGKNDSTETAGVTRPLQTTESPTTSRRQEEKQIWNIFSVQKGKENVAPGTLATTPVAKDETSAPSVSEVPISQKPQSEQNIAGVGAEILNVAEGIRNFVHGWEDNIANSTKQTPTPDTEMVTVTTAPSLTVNDTGTLSYNKTEPSENTMANHTEGPVSEPHKLISSHNKPLLPDNFNPEVGLLQLIGEPVPEQITKVFGPDKTPAYVFGKEANTGQVAQYHLPNPMYRDFSLLFHVQPTTDKAGILFAITDASQSIVYIGVKLSEVKDGKQDIIFYYTEPGSQDSYVAATFNVPSLVNRWSRFAISVEEEEVVLFMDCDELERVRFERSPDEMELEDGSGLFVAQAGGADPDRYQGVIADLKIVADPRAAEHQCEEDGDDSDGISGDFGSGIADNDLVTSPPVIIKVQPPITVPPIQRTPVEKEIVQTGQGQQGVKGEKGDRGEKGERGLTGPKGDGGNASPTSDSKGEKGQKGEVGVKGSAGFGYPGPKGQKGDPGDLGPRGPPGPPGPSILQKSDGTTVQQVTGPPGPQGPAGPPGKEGEPGDPGEDGKTGEVGPQGFPGTPGDSGQKGDKGDSGVGIRGAPGPPGPPGPPGPSRTDKLTFIDMEGSGYGGDSEHIRGAPGVPGPPGPPGVPGLPGVPGTFGMNNTGLPGPPGLPGLPGKEGIPGPPGPPGLPGKDGLSGTPGAAGLRGEDGEIGLPGAPGTKGSTGEPGLPGPPGQSGLAGLPGPMGPRGLPGPPGPPGPVGHGSAIGFDDMEASGLGFGLREGTQGVPGLPGAKGEPGHPGLPGVKGDSGFPGLEGRPGLDGFPGPEGLKGEKGERGERGESGRDGVGIPGPPGPPGQVLYLPSDDKTIVGAIPGPEGRQGVAGLPGPRGPKGEAGLNGIPGTFGVKGEKGEPGIIMGPDSTIYTDLSKGEKGDEGPRGPAGAAGPFGQKGEIGFPGRPGRPGMNGLKGEKGEPSGASGGFGLRGPPGPPGPPGPPGSVVYGDPNNPGAQVYPGHKGERGFPGPKGDVGPAGPPGSFPYDLSTFASILRREAGLKGEKGESGGGSHGQGIPGPPGNPGYPGLPGAKGDSVQGPPGPAGPPGPSGIGYEGRQGPPGPPGPPGLPGSSSYPGPHRQTISIPGPPGPPGPPGLPGTSGGLSGATVLQTYQTMINLAHRLQEGTIVLVQERSELYVRVREGFRKILLGEYTTSSGPGQDNEVAAEQAPVVPYPHRNKDSDYLQSTEENSNNPNVPSGARPWSDPRLPEPPRVNPPPPPDHHHHGNPRPLEPSPVHTHQEFNPVLHLVALNTPLSGSMKSIRGVDFQCFEQARKAGLHGTFRAFLSSRLQDLYSIVRRADRQSVQIVNLRDEVLFDNWESLFSGSEAQMRPGASIFSFDGKDVATDPTWPQKMVWHGSDSKGRRLTESYCETWRTDEGAVTGQASSLTSGKLLEQRPQSCNKNFIVLCIENSFMTNNRK

Target ORF information:

RefSeq Version	XM_012970903.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen type XVIII alpha 1 (col18a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970903.3

ORF Insert Sequence:

ATGGCTCCAG CGCGGCTTAG CCTTGGGGTC ACAGTGCTTG TGCTCCTCTT ACCTGCTCTA 
TCCAATGGCC AGTTTTGGTC CTGGTTTACA GGGAGCAAAG AGACAACTGC GAGCCCCACA 
ACTCAACGTG TAACTCCAGG AACATCCTCA GTTCATAGTA CTGCTGTGCA GCATCACTCA 
CCTGATGCCT CGCCAACCAC ATTGCACCAT ATACCGGATG CACTACCCTC AGCGGAAAGT 
ACGTCAGCAC CTGCAGTGCA TCAGCCACCT GAAGAAGATA ACCTGCCTGA GACAACACCC 
TCTCACCTAG AGGCAGCCTC TGTGCCATCT TCAGAAAAAC CCACACCTTT TACAGAACAG 
TCACCCACAG CATCCTTCTC AGCCATACTC ATTCCCAGCA CAGTCATCCT AACCACAGAG 
GGCAAGAATG ACAGTACTGA GACAGCAGGA GTGACCAGAC CCCTCCAAAC GACAGAGTCC 
CCTACTACTT CCAGGAGGCA GGAGGAAAAG CAAATATGGA ACATATTCAG TGTGCAGAAA 
GGAAAGGAGA ACGTAGCGCC TGGGACTTTG GCCACAACAC CTGTAGCGAA GGATGAAACC 
AGTGCACCTT CTGTGTCAGA GGTTCCAATA TCTCAGAAGC CACAAAGTGA ACAAAACATT 
GCTGGTGTAG GGGCCGAGAT CTTAAATGTA GCAGAAGGAA TTAGGAACTT TGTGCATGGC 
TGGGAAGATA ACATTGCCAA CAGCACAAAG CAGACTCCTA CTCCTGACAC GGAGATGGTG 
ACTGTCACAA CAGCCCCAAG CCTTACTGTA AATGACACTG GCACCTTAAG CTACAACAAG 
ACAGAACCAA GCGAGAACAC TATGGCAAAC CATACGGAAG GTCCGGTGTC CGAGCCCCAT 
AAACTGATCT CATCCCACAA CAAGCCACTT CTACCAGACA ACTTCAACCC AGAAGTTGGC 
CTCTTGCAGT TGATTGGAGA ACCTGTACCT GAACAGATCA CCAAGGTCTT TGGGCCCGAC 
AAGACTCCCG CCTATGTATT TGGTAAAGAA GCCAATACAG GCCAGGTGGC CCAATACCAT 
CTGCCCAACC CTATGTATAG GGACTTCTCT CTCCTCTTCC ATGTCCAGCC AACCACAGAC 
AAGGCCGGCA TCCTCTTTGC CATCACAGAT GCCTCTCAGT CTATTGTGTA TATCGGGGTG 
AAGCTTTCTG AAGTCAAGGA TGGGAAGCAA GACATCATCT TCTACTATAC AGAGCCTGGC 
TCACAGGATT CCTATGTAGC TGCCACCTTC AATGTCCCAT CACTGGTGAA TCGTTGGTCT 
CGCTTTGCAA TCAGTGTGGA GGAAGAAGAA GTTGTGCTGT TTATGGATTG TGATGAGCTG 
GAAAGGGTGC GCTTCGAGAG ATCACCTGAT GAGATGGAAC TAGAAGATGG CTCTGGGCTC 
TTTGTTGCTC AAGCAGGTGG CGCTGATCCA GACAGATATC AGGGTGTAAT AGCAGATCTA 
AAGATCGTAG CCGATCCAAG AGCCGCAGAA CATCAGTGTG AGGAGGATGG AGATGACTCT 
GATGGGATCT CTGGAGATTT TGGCAGTGGC ATTGCGGACA ATGATTTGGT GACTTCTCCT 
CCTGTGATAA TCAAAGTGCA GCCTCCTATA ACTGTCCCTC CCATACAGAG GACCCCTGTG 
GAGAAAGAGA TTGTTCAGAC AGGTCAAGGC CAGCAAGGGG TAAAAGGAGA GAAGGGTGAT 
CGTGGAGAGA AGGGTGAGAG GGGACTGACT GGCCCTAAGG GAGACGGTGG GAATGCAAGT 
CCAACTTCTG ATTCCAAAGG AGAAAAGGGT CAGAAAGGAG AGGTCGGAGT GAAGGGAAGT 
GCAGGCTTTG GCTATCCCGG GCCTAAAGGG CAGAAAGGAG ATCCTGGAGA CTTGGGACCA 
AGAGGACCAC CAGGCCCACC TGGACCATCT ATATTACAGA AAAGTGATGG AACTACAGTG 
CAACAGGTCA CAGGACCTCC AGGTCCTCAA GGCCCTGCGG GACCACCAGG AAAAGAAGGG 
GAACCAGGTG ACCCTGGTGA AGATGGAAAA ACTGGCGAGG TTGGTCCCCA AGGATTTCCA 
GGGACCCCAG GAGATTCTGG GCAGAAGGGA GACAAGGGTG ATTCTGGTGT TGGTATCAGA 
GGTGCTCCTG GGCCACCAGG TCCGCCAGGC CCCCCTGGAC CTTCTAGAAC CGACAAGCTG 
ACATTCATTG ACATGGAAGG TTCTGGATAT GGTGGAGATT CGGAGCACAT CAGGGGAGCC 
CCTGGTGTGC CTGGGCCCCC AGGTCCTCCA GGTGTACCAG GGTTACCCGG AGTACCCGGA 
ACTTTTGGCA TGAACAACAC AGGATTGCCA GGTCCACCTG GATTGCCAGG TCTTCCCGGA 
AAGGAAGGAA TTCCAGGTCC TCCTGGCCCC CCAGGACTCC CTGGAAAAGA CGGGTTGTCT 
GGTACACCTG GTGCTGCTGG ATTACGGGGT GAAGATGGAG AGATTGGTCT TCCAGGTGCT 
CCTGGAACAA AGGGTAGCAC TGGTGAGCCA GGACTACCTG GACCTCCTGG ACAATCTGGT 
TTAGCTGGAC TTCCTGGACC AATGGGACCA CGCGGGCTGC CAGGTCCTCC AGGACCACCT 
GGGCCTGTGG GTCATGGATC TGCAATTGGC TTTGATGACA TGGAAGCCTC AGGGCTTGGA 
TTTGGCTTGA GAGAAGGGAC ACAGGGGGTG CCTGGACTTC CCGGGGCAAA GGGAGAGCCA 
GGACATCCAG GGCTGCCAGG AGTGAAGGGA GATAGTGGTT TTCCAGGCCT TGAAGGAAGG 
CCGGGGTTGG ATGGATTCCC AGGTCCAGAG GGATTAAAGG GTGAGAAAGG TGAACGTGGT 
GAGCGGGGAG AATCTGGCCG TGATGGTGTT GGTATTCCAG GTCCACCGGG ACCTCCTGGT 
CAGGTGCTTT ATTTACCATC CGATGACAAG ACTATTGTTG GTGCCATTCC TGGACCAGAG 
GGCAGGCAAG GTGTAGCTGG ACTTCCGGGC CCAAGGGGAC CCAAAGGAGA GGCGGGCTTG 
AACGGTATCC CAGGAACCTT TGGAGTAAAG GGAGAAAAGG GAGAGCCTGG AATTATTATG 
GGACCAGATA GCACCATTTA CACTGATCTA TCTAAAGGAG AGAAGGGTGA CGAAGGACCA 
AGGGGACCAG CAGGAGCTGC GGGACCATTT GGACAAAAGG GAGAAATCGG GTTCCCAGGA 
CGCCCTGGAC GTCCTGGTAT GAATGGACTG AAAGGGGAGA AAGGAGAACC TAGTGGTGCA 
AGTGGTGGCT TTGGACTGAG GGGGCCACCT GGTCCTCCTG GCCCACCCGG TCCTCCTGGC 
AGTGTGGTGT ATGGAGATCC AAATAACCCA GGAGCCCAAG TATATCCAGG TCATAAAGGG 
GAGAGAGGAT TTCCTGGACC CAAAGGTGAT GTTGGTCCTG CTGGCCCACC AGGCTCTTTC 
CCATATGATT TAAGTACCTT TGCCTCGATT TTAAGGAGAG AAGCTGGACT CAAAGGAGAG 
AAGGGCGAAT CTGGGGGTGG TTCCCATGGA CAGGGGATCC CAGGGCCACC TGGAAACCCT 
GGATATCCAG GACTGCCGGG AGCTAAAGGA GACAGTGTTC AAGGTCCTCC AGGCCCAGCC 
GGCCCACCAG GACCTTCTGG AATCGGTTAT GAAGGAAGGC AAGGTCCACC AGGGCCCCCA 
GGTCCTCCAG GCCTGCCAGG TTCCTCCTCT TATCCTGGGC CTCACAGGCA AACCATCAGC 
ATACCTGGAC CTCCTGGCCC ACCAGGCCCA CCAGGGCTCC CAGGAACATC AGGAGGATTA 
TCTGGGGCTA CTGTGCTACA GACCTATCAG ACTATGATCA ACTTAGCCCA CAGGCTGCAG 
GAAGGGACAA TTGTGCTGGT CCAGGAGAGG TCGGAACTGT ATGTGCGTGT GAGAGAAGGC 
TTCCGAAAAA TTCTTCTTGG TGAATATACA ACATCCTCTG GCCCTGGACA GGATAATGAA 
GTTGCTGCGG AGCAGGCCCC TGTAGTTCCA TATCCACACC GTAACAAAGA TTCTGATTAC 
CTCCAGTCTA CAGAGGAGAA CAGCAACAAT CCAAATGTGC CCTCAGGTGC CCGACCCTGG 
AGCGATCCTC GGTTACCAGA ACCACCAAGA GTAAACCCAC CACCACCCCC AGACCATCAC 
CACCATGGAA ACCCTCGTCC ATTGGAACCA TCCCCAGTGC ACACACACCA AGAGTTTAAT 
CCTGTGCTTC ACCTTGTGGC CCTGAACACT CCACTGAGTG GCAGCATGAA ATCCATCCGC 
GGAGTGGACT TCCAGTGCTT TGAGCAAGCC CGTAAAGCTG GACTACATGG CACATTTAGA 
GCGTTTTTGT CTTCCCGACT TCAAGACCTG TACAGCATTG TACGCAGAGC TGATCGACAA 
TCTGTGCAAA TTGTTAATCT TAGGGATGAA GTGTTATTTG ACAACTGGGA GTCCCTGTTC 
TCTGGTTCTG AGGCTCAGAT GAGGCCTGGT GCGAGTATCT TCTCTTTTGA TGGAAAAGAT 
GTTGCAACAG ATCCTACATG GCCACAGAAG ATGGTTTGGC ATGGGTCTGA CTCGAAGGGA 
CGGCGGCTTA CTGAAAGCTA TTGCGAGACC TGGCGCACAG ATGAAGGTGC AGTAACTGGT 
CAGGCATCAT CGCTGACGAG CGGGAAACTC TTAGAACAGC GCCCACAGAG TTGCAACAAG 
AACTTCATTG TTTTGTGTAT TGAGAACAGC TTCATGACCA ATAACCGGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col18a1 ( XM_012970904.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826358.2 Xenopus tropicalis collagen type XVIII alpha 1 (col18a1), transcript variant X2, mRNA.

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CloneID	OXa66570
Clone ID Related Accession (Same CDS sequence)	XM_012970904.3
Accession Version	XM_012970904.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(XVIII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970904.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCAG CGCGGCTTAG CCTTGGGGTC ACAGTGCTTG TGCTCCTCTT ACCTGCTCTA 
TCCAATGGCC AGTTTTGGTC CTGGTTTACA GGGAGCAAAG AGACAACTGC GAGCCCCACA 
ACTCAACGTG TAACTCCAGG AACATCCTCA GTTCATAGTA CTGCTGTGCA GCATCACTCA 
CCTGATGCCT CGCCAACCAC ATTGCACCAT ATACCGGATG CACTACCCTC AGCGGAAAGT 
ACGTCAGCAC CTGCAGTGCA TCAGCCACCT GAAGAAGATA ACCTGCCTGA GACAACACCC 
TCTCACCTAG AGGCAGCCTC TGTGCCATCT TCAGAAAAAC CCACACCTTT TACAGAACAG 
TCACCCACAG CATCCTTCTC AGCCATACTC ATTCCCAGCA CAGTCATCCT AACCACAGAG 
GGCAAGAATG ACAGTACTGA GACAGCAGGA GTGACCAGAC CCCTCCAAAC GACAGAGTCC 
CCTACTACTT CCAGGAGGCA GGAGGAAAAG CAAATATGGA ACATATTCAG TGTGCAGAAA 
GGAAAGGAGA ACGTAGCGCC TGGGACTTTG GCCACAACAC CTGTAGCGAA GGATGAAACC 
AGTGCACCTT CTGTGTCAGA GGTTCCAATA TCTCAGAAGC CACAAAGTGA ACAAAACATT 
GCTGGTGTAG GGGCCGAGAT CTTAAATGTA GCAGAAGGAA TTAGGAACTT TGTGCATGGC 
TGGGAAGATA ACATTGCCAA CAGCACAAAG CAGACTCCTA CTCCTGACAC GGAGATGGTG 
ACTGTCACAA CAGCCCCAAG CCTTACTGTA AATGACACTG GCACCTTAAG CTACAACAAG 
ACAGAACCAA GCGAGAACAC TATGGCAAAC CATACGGAAG GTCCGGTGTC CGAGCCCCAT 
AAACTGATCT CATCCCACAA CAAGCCACTT CTACCAGACA ACTTCAACCC AGAAGTTGGC 
CTCTTGCAGT TGATTGGAGA ACCTGTACCT GAACAGATCA CCAAGGTCTT TGGGCCCGAC 
AAGACTCCCG CCTATGTATT TGGTAAAGAA GCCAATACAG GCCAGGTGGC CCAATACCAT 
CTGCCCAACC CTATGTATAG GGACTTCTCT CTCCTCTTCC ATGTCCAGCC AACCACAGAC 
AAGGCCGGCA TCCTCTTTGC CATCACAGAT GCCTCTCAGT CTATTGTGTA TATCGGGGTG 
AAGCTTTCTG AAGTCAAGGA TGGGAAGCAA GACATCATCT TCTACTATAC AGAGCCTGGC 
TCACAGGATT CCTATGTAGC TGCCACCTTC AATGTCCCAT CACTGGTGAA TCGTTGGTCT 
CGCTTTGCAA TCAGTGTGGA GGAAGAAGAA GTTGTGCTGT TTATGGATTG TGATGAGCTG 
GAAAGGGTGC GCTTCGAGAG ATCACCTGAT GAGATGGAAC TAGAAGATGG CTCTGGGCTC 
TTTGTTGCTC AAGCAGGTGG CGCTGATCCA GACAGATATC AGGGTGTAAT AGCAGATCTA 
AAGATCGTAG CCGATCCAAG AGCCGCAGAA CATCAGTGTG AGGAGGATGG AGATGACTCT 
GATGGGATCT CTGGAGATTT TGGCAGTGGC ATTGCGGACA ATGATTTGGT GACTTCTCCT 
CCTGTGATAA TCAAAGTGCA GCCTCCTATA ACTGTCCCTC CCATACAGAG GACCCCTGTG 
GAGAAAGAGA TTGTTCAGAC AGGTCAAGGC CAGCAAGGGG TAAAAGGAGA GAAGGGTGAT 
CGTGGAGAGA AGGGTGAGAG GGGACTGACT GGCCCTAAGG GAGACGGTGG GAATGCAAGT 
CCAACTTCTG ATTCCAAAGG AGAAAAGGGT CAGAAAGGAG AGGTCGGAGT GAAGGGAAGT 
GCAGGCTTTG GCTATCCCGG GCCTAAAGGG CAGAAAGGAG ATCCTGGAGA CTTGGGACCA 
AGAGGACCAC CAGGCCCACC TGGACCATCT ATATTACAGA AAAGTGATGG AACTACAGTG 
CAACAGGTCA CAGGACCTCC AGGTCCTCAA GGCCCTGCGG GACCACCAGG AAAAGAAGGG 
GAACCAGGTG ACCCTGGTGA AGATGGAAAA ACTGGCGAGG TTGGTCCCCA AGGATTTCCA 
GGGACCCCAG GAGATTCTGG GCAGAAGGGA GACAAGGGTG ATTCTGGTGT TGGTATCAGA 
GGTGCTCCTG GGCCACCAGG TCCGCCAGGC CCCCCTGGAC CTTCTAGAAC CGACAAGCTG 
ACATTCATTG ACATGGAAGG TTCTGGATAT GGTGGAGATT CGGAGCACAT CAGGGGAGCC 
CCTGGTGTGC CTGGGCCCCC AGGTCCTCCA GGTGTACCAG GGTTACCCGG AGTACCCGGA 
ACTTTTGGCA TGAACAACAC AGGATTGCCA GGTCCACCTG GATTGCCAGG TCTTCCCGGA 
AAGGAAGGAA TTCCAGGTCC TCCTGGCCCC CCAGGACTCC CTGGAAAAGA CGGGTTGTCT 
GGTACACCTG GTGCTGCTGG ATTACGGGGT GAAGATGGAG AGATTGGTCT TCCAGGTGCT 
CCTGGAACAA AGGGTAGCAC TGGTGAGCCA GGACTACCTG GACCTCCTGG ACAATCTGGT 
TTAGCTGGAC TTCCTGGACC AATGGGACCA CGCGGGCTGC CAGGTCCTCC AGGACCACCT 
GGGCCTGTGG GTCATGGATC TGCAATTGGC TTTGATGACA TGGAAGCCTC AGGGCTTGGA 
TTTGGCTTGA GAGAAGGGAC ACAGGGGGTG CCTGGACTTC CCGGGGCAAA GGGAGAGCCA 
GGACATCCAG GGCTGCCAGG AGTGAAGGGA GATAGTGGTT TTCCAGGCCT TGAAGGAAGG 
CCGGGGTTGG ATGGATTCCC AGGTCCAGAG GGATTAAAGG GTGAGAAAGG TGAACGTGGT 
GAGCGGGGAG AATCTGGCCG TGATGGTGTT GGTATTCCAG GTCCACCGGG ACCTCCTGGT 
CAGGTGCTTT ATTTACCATC CGATGACAAG ACTATTGTTG GTGCCATTCC TGGACCAGAG 
GGCCCAAGGG GACCCAAAGG AGAGGCGGGC TTGAACGGTA TCCCAGGAAC CTTTGGAGTA 
AAGGGAGAAA AGGGAGAGCC TGGAATTATT ATGGGACCAG ATAGCACCAT TTACACTGAT 
CTATCTAAAG GAGAGAAGGG TGACGAAGGA CCAAGGGGAC CAGCAGGAGC TGCGGGACCA 
TTTGGACAAA AGGGAGAAAT CGGGTTCCCA GGACGCCCTG GACGTCCTGG TATGAATGGA 
CTGAAAGGGG AGAAAGGAGA ACCTAGTGGT GCAAGTGGTG GCTTTGGACT GAGGGGGCCA 
CCTGGTCCTC CTGGCCCACC CGGTCCTCCT GGCAGTGTGG TGTATGGAGA TCCAAATAAC 
CCAGGAGCCC AAGTATATCC AGGTCATAAA GGGGAGAGAG GATTTCCTGG ACCCAAAGGT 
GATGTTGGTC CTGCTGGCCC ACCAGGCTCT TTCCCATATG ATTTAAGTAC CTTTGCCTCG 
ATTTTAAGGA GAGAAGCTGG ACTCAAAGGA GAGAAGGGCG AATCTGGGGG TGGTTCCCAT 
GGACAGGGGA TCCCAGGGCC ACCTGGAAAC CCTGGATATC CAGGACTGCC GGGAGCTAAA 
GGAGACAGTG TTCAAGGTCC TCCAGGCCCA GCCGGCCCAC CAGGACCTTC TGGAATCGGT 
TATGAAGGAA GGCAAGGTCC ACCAGGGCCC CCAGGTCCTC CAGGCCTGCC AGGTTCCTCC 
TCTTATCCTG GGCCTCACAG GCAAACCATC AGCATACCTG GACCTCCTGG CCCACCAGGC 
CCACCAGGGC TCCCAGGAAC ATCAGGAGGA TTATCTGGGG CTACTGTGCT ACAGACCTAT 
CAGACTATGA TCAACTTAGC CCACAGGCTG CAGGAAGGGA CAATTGTGCT GGTCCAGGAG 
AGGTCGGAAC TGTATGTGCG TGTGAGAGAA GGCTTCCGAA AAATTCTTCT TGGTGAATAT 
ACAACATCCT CTGGCCCTGG ACAGGATAAT GAAGTTGCTG CGGAGCAGGC CCCTGTAGTT 
CCATATCCAC ACCGTAACAA AGATTCTGAT TACCTCCAGT CTACAGAGGA GAACAGCAAC 
AATCCAAATG TGCCCTCAGG TGCCCGACCC TGGAGCGATC CTCGGTTACC AGAACCACCA 
AGAGTAAACC CACCACCACC CCCAGACCAT CACCACCATG GAAACCCTCG TCCATTGGAA 
CCATCCCCAG TGCACACACA CCAAGAGTTT AATCCTGTGC TTCACCTTGT GGCCCTGAAC 
ACTCCACTGA GTGGCAGCAT GAAATCCATC CGCGGAGTGG ACTTCCAGTG CTTTGAGCAA 
GCCCGTAAAG CTGGACTACA TGGCACATTT AGAGCGTTTT TGTCTTCCCG ACTTCAAGAC 
CTGTACAGCA TTGTACGCAG AGCTGATCGA CAATCTGTGC AAATTGTTAA TCTTAGGGAT 
GAAGTGTTAT TTGACAACTG GGAGTCCCTG TTCTCTGGTT CTGAGGCTCA GATGAGGCCT 
GGTGCGAGTA TCTTCTCTTT TGATGGAAAA GATGTTGCAA CAGATCCTAC ATGGCCACAG 
AAGATGGTTT GGCATGGGTC TGACTCGAAG GGACGGCGGC TTACTGAAAG CTATTGCGAG 
ACCTGGCGCA CAGATGAAGG TGCAGTAACT GGTCAGGCAT CATCGCTGAC GAGCGGGAAA 
CTCTTAGAAC AGCGCCCACA GAGTTGCAAC AAGAACTTCA TTGTTTTGTG TATTGAGAAC 
AGCTTCATGA CCAATAACCG GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826358.2
CDS	56..4822
Translation	MAPARLSLGVTVLVLLLPALSNGQFWSWFTGSKETTASPTTQRVTPGTSSVHSTAVQHHSPDASPTTLHHIPDALPSAESTSAPAVHQPPEEDNLPETTPSHLEAASVPSSEKPTPFTEQSPTASFSAILIPSTVILTTEGKNDSTETAGVTRPLQTTESPTTSRRQEEKQIWNIFSVQKGKENVAPGTLATTPVAKDETSAPSVSEVPISQKPQSEQNIAGVGAEILNVAEGIRNFVHGWEDNIANSTKQTPTPDTEMVTVTTAPSLTVNDTGTLSYNKTEPSENTMANHTEGPVSEPHKLISSHNKPLLPDNFNPEVGLLQLIGEPVPEQITKVFGPDKTPAYVFGKEANTGQVAQYHLPNPMYRDFSLLFHVQPTTDKAGILFAITDASQSIVYIGVKLSEVKDGKQDIIFYYTEPGSQDSYVAATFNVPSLVNRWSRFAISVEEEEVVLFMDCDELERVRFERSPDEMELEDGSGLFVAQAGGADPDRYQGVIADLKIVADPRAAEHQCEEDGDDSDGISGDFGSGIADNDLVTSPPVIIKVQPPITVPPIQRTPVEKEIVQTGQGQQGVKGEKGDRGEKGERGLTGPKGDGGNASPTSDSKGEKGQKGEVGVKGSAGFGYPGPKGQKGDPGDLGPRGPPGPPGPSILQKSDGTTVQQVTGPPGPQGPAGPPGKEGEPGDPGEDGKTGEVGPQGFPGTPGDSGQKGDKGDSGVGIRGAPGPPGPPGPPGPSRTDKLTFIDMEGSGYGGDSEHIRGAPGVPGPPGPPGVPGLPGVPGTFGMNNTGLPGPPGLPGLPGKEGIPGPPGPPGLPGKDGLSGTPGAAGLRGEDGEIGLPGAPGTKGSTGEPGLPGPPGQSGLAGLPGPMGPRGLPGPPGPPGPVGHGSAIGFDDMEASGLGFGLREGTQGVPGLPGAKGEPGHPGLPGVKGDSGFPGLEGRPGLDGFPGPEGLKGEKGERGERGESGRDGVGIPGPPGPPGQVLYLPSDDKTIVGAIPGPEGPRGPKGEAGLNGIPGTFGVKGEKGEPGIIMGPDSTIYTDLSKGEKGDEGPRGPAGAAGPFGQKGEIGFPGRPGRPGMNGLKGEKGEPSGASGGFGLRGPPGPPGPPGPPGSVVYGDPNNPGAQVYPGHKGERGFPGPKGDVGPAGPPGSFPYDLSTFASILRREAGLKGEKGESGGGSHGQGIPGPPGNPGYPGLPGAKGDSVQGPPGPAGPPGPSGIGYEGRQGPPGPPGPPGLPGSSSYPGPHRQTISIPGPPGPPGPPGLPGTSGGLSGATVLQTYQTMINLAHRLQEGTIVLVQERSELYVRVREGFRKILLGEYTTSSGPGQDNEVAAEQAPVVPYPHRNKDSDYLQSTEENSNNPNVPSGARPWSDPRLPEPPRVNPPPPPDHHHHGNPRPLEPSPVHTHQEFNPVLHLVALNTPLSGSMKSIRGVDFQCFEQARKAGLHGTFRAFLSSRLQDLYSIVRRADRQSVQIVNLRDEVLFDNWESLFSGSEAQMRPGASIFSFDGKDVATDPTWPQKMVWHGSDSKGRRLTESYCETWRTDEGAVTGQASSLTSGKLLEQRPQSCNKNFIVLCIENSFMTNNRK

Target ORF information:

RefSeq Version	XM_012970904.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen type XVIII alpha 1 (col18a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970904.3

ORF Insert Sequence:

ATGGCTCCAG CGCGGCTTAG CCTTGGGGTC ACAGTGCTTG TGCTCCTCTT ACCTGCTCTA 
TCCAATGGCC AGTTTTGGTC CTGGTTTACA GGGAGCAAAG AGACAACTGC GAGCCCCACA 
ACTCAACGTG TAACTCCAGG AACATCCTCA GTTCATAGTA CTGCTGTGCA GCATCACTCA 
CCTGATGCCT CGCCAACCAC ATTGCACCAT ATACCGGATG CACTACCCTC AGCGGAAAGT 
ACGTCAGCAC CTGCAGTGCA TCAGCCACCT GAAGAAGATA ACCTGCCTGA GACAACACCC 
TCTCACCTAG AGGCAGCCTC TGTGCCATCT TCAGAAAAAC CCACACCTTT TACAGAACAG 
TCACCCACAG CATCCTTCTC AGCCATACTC ATTCCCAGCA CAGTCATCCT AACCACAGAG 
GGCAAGAATG ACAGTACTGA GACAGCAGGA GTGACCAGAC CCCTCCAAAC GACAGAGTCC 
CCTACTACTT CCAGGAGGCA GGAGGAAAAG CAAATATGGA ACATATTCAG TGTGCAGAAA 
GGAAAGGAGA ACGTAGCGCC TGGGACTTTG GCCACAACAC CTGTAGCGAA GGATGAAACC 
AGTGCACCTT CTGTGTCAGA GGTTCCAATA TCTCAGAAGC CACAAAGTGA ACAAAACATT 
GCTGGTGTAG GGGCCGAGAT CTTAAATGTA GCAGAAGGAA TTAGGAACTT TGTGCATGGC 
TGGGAAGATA ACATTGCCAA CAGCACAAAG CAGACTCCTA CTCCTGACAC GGAGATGGTG 
ACTGTCACAA CAGCCCCAAG CCTTACTGTA AATGACACTG GCACCTTAAG CTACAACAAG 
ACAGAACCAA GCGAGAACAC TATGGCAAAC CATACGGAAG GTCCGGTGTC CGAGCCCCAT 
AAACTGATCT CATCCCACAA CAAGCCACTT CTACCAGACA ACTTCAACCC AGAAGTTGGC 
CTCTTGCAGT TGATTGGAGA ACCTGTACCT GAACAGATCA CCAAGGTCTT TGGGCCCGAC 
AAGACTCCCG CCTATGTATT TGGTAAAGAA GCCAATACAG GCCAGGTGGC CCAATACCAT 
CTGCCCAACC CTATGTATAG GGACTTCTCT CTCCTCTTCC ATGTCCAGCC AACCACAGAC 
AAGGCCGGCA TCCTCTTTGC CATCACAGAT GCCTCTCAGT CTATTGTGTA TATCGGGGTG 
AAGCTTTCTG AAGTCAAGGA TGGGAAGCAA GACATCATCT TCTACTATAC AGAGCCTGGC 
TCACAGGATT CCTATGTAGC TGCCACCTTC AATGTCCCAT CACTGGTGAA TCGTTGGTCT 
CGCTTTGCAA TCAGTGTGGA GGAAGAAGAA GTTGTGCTGT TTATGGATTG TGATGAGCTG 
GAAAGGGTGC GCTTCGAGAG ATCACCTGAT GAGATGGAAC TAGAAGATGG CTCTGGGCTC 
TTTGTTGCTC AAGCAGGTGG CGCTGATCCA GACAGATATC AGGGTGTAAT AGCAGATCTA 
AAGATCGTAG CCGATCCAAG AGCCGCAGAA CATCAGTGTG AGGAGGATGG AGATGACTCT 
GATGGGATCT CTGGAGATTT TGGCAGTGGC ATTGCGGACA ATGATTTGGT GACTTCTCCT 
CCTGTGATAA TCAAAGTGCA GCCTCCTATA ACTGTCCCTC CCATACAGAG GACCCCTGTG 
GAGAAAGAGA TTGTTCAGAC AGGTCAAGGC CAGCAAGGGG TAAAAGGAGA GAAGGGTGAT 
CGTGGAGAGA AGGGTGAGAG GGGACTGACT GGCCCTAAGG GAGACGGTGG GAATGCAAGT 
CCAACTTCTG ATTCCAAAGG AGAAAAGGGT CAGAAAGGAG AGGTCGGAGT GAAGGGAAGT 
GCAGGCTTTG GCTATCCCGG GCCTAAAGGG CAGAAAGGAG ATCCTGGAGA CTTGGGACCA 
AGAGGACCAC CAGGCCCACC TGGACCATCT ATATTACAGA AAAGTGATGG AACTACAGTG 
CAACAGGTCA CAGGACCTCC AGGTCCTCAA GGCCCTGCGG GACCACCAGG AAAAGAAGGG 
GAACCAGGTG ACCCTGGTGA AGATGGAAAA ACTGGCGAGG TTGGTCCCCA AGGATTTCCA 
GGGACCCCAG GAGATTCTGG GCAGAAGGGA GACAAGGGTG ATTCTGGTGT TGGTATCAGA 
GGTGCTCCTG GGCCACCAGG TCCGCCAGGC CCCCCTGGAC CTTCTAGAAC CGACAAGCTG 
ACATTCATTG ACATGGAAGG TTCTGGATAT GGTGGAGATT CGGAGCACAT CAGGGGAGCC 
CCTGGTGTGC CTGGGCCCCC AGGTCCTCCA GGTGTACCAG GGTTACCCGG AGTACCCGGA 
ACTTTTGGCA TGAACAACAC AGGATTGCCA GGTCCACCTG GATTGCCAGG TCTTCCCGGA 
AAGGAAGGAA TTCCAGGTCC TCCTGGCCCC CCAGGACTCC CTGGAAAAGA CGGGTTGTCT 
GGTACACCTG GTGCTGCTGG ATTACGGGGT GAAGATGGAG AGATTGGTCT TCCAGGTGCT 
CCTGGAACAA AGGGTAGCAC TGGTGAGCCA GGACTACCTG GACCTCCTGG ACAATCTGGT 
TTAGCTGGAC TTCCTGGACC AATGGGACCA CGCGGGCTGC CAGGTCCTCC AGGACCACCT 
GGGCCTGTGG GTCATGGATC TGCAATTGGC TTTGATGACA TGGAAGCCTC AGGGCTTGGA 
TTTGGCTTGA GAGAAGGGAC ACAGGGGGTG CCTGGACTTC CCGGGGCAAA GGGAGAGCCA 
GGACATCCAG GGCTGCCAGG AGTGAAGGGA GATAGTGGTT TTCCAGGCCT TGAAGGAAGG 
CCGGGGTTGG ATGGATTCCC AGGTCCAGAG GGATTAAAGG GTGAGAAAGG TGAACGTGGT 
GAGCGGGGAG AATCTGGCCG TGATGGTGTT GGTATTCCAG GTCCACCGGG ACCTCCTGGT 
CAGGTGCTTT ATTTACCATC CGATGACAAG ACTATTGTTG GTGCCATTCC TGGACCAGAG 
GGCCCAAGGG GACCCAAAGG AGAGGCGGGC TTGAACGGTA TCCCAGGAAC CTTTGGAGTA 
AAGGGAGAAA AGGGAGAGCC TGGAATTATT ATGGGACCAG ATAGCACCAT TTACACTGAT 
CTATCTAAAG GAGAGAAGGG TGACGAAGGA CCAAGGGGAC CAGCAGGAGC TGCGGGACCA 
TTTGGACAAA AGGGAGAAAT CGGGTTCCCA GGACGCCCTG GACGTCCTGG TATGAATGGA 
CTGAAAGGGG AGAAAGGAGA ACCTAGTGGT GCAAGTGGTG GCTTTGGACT GAGGGGGCCA 
CCTGGTCCTC CTGGCCCACC CGGTCCTCCT GGCAGTGTGG TGTATGGAGA TCCAAATAAC 
CCAGGAGCCC AAGTATATCC AGGTCATAAA GGGGAGAGAG GATTTCCTGG ACCCAAAGGT 
GATGTTGGTC CTGCTGGCCC ACCAGGCTCT TTCCCATATG ATTTAAGTAC CTTTGCCTCG 
ATTTTAAGGA GAGAAGCTGG ACTCAAAGGA GAGAAGGGCG AATCTGGGGG TGGTTCCCAT 
GGACAGGGGA TCCCAGGGCC ACCTGGAAAC CCTGGATATC CAGGACTGCC GGGAGCTAAA 
GGAGACAGTG TTCAAGGTCC TCCAGGCCCA GCCGGCCCAC CAGGACCTTC TGGAATCGGT 
TATGAAGGAA GGCAAGGTCC ACCAGGGCCC CCAGGTCCTC CAGGCCTGCC AGGTTCCTCC 
TCTTATCCTG GGCCTCACAG GCAAACCATC AGCATACCTG GACCTCCTGG CCCACCAGGC 
CCACCAGGGC TCCCAGGAAC ATCAGGAGGA TTATCTGGGG CTACTGTGCT ACAGACCTAT 
CAGACTATGA TCAACTTAGC CCACAGGCTG CAGGAAGGGA CAATTGTGCT GGTCCAGGAG 
AGGTCGGAAC TGTATGTGCG TGTGAGAGAA GGCTTCCGAA AAATTCTTCT TGGTGAATAT 
ACAACATCCT CTGGCCCTGG ACAGGATAAT GAAGTTGCTG CGGAGCAGGC CCCTGTAGTT 
CCATATCCAC ACCGTAACAA AGATTCTGAT TACCTCCAGT CTACAGAGGA GAACAGCAAC 
AATCCAAATG TGCCCTCAGG TGCCCGACCC TGGAGCGATC CTCGGTTACC AGAACCACCA 
AGAGTAAACC CACCACCACC CCCAGACCAT CACCACCATG GAAACCCTCG TCCATTGGAA 
CCATCCCCAG TGCACACACA CCAAGAGTTT AATCCTGTGC TTCACCTTGT GGCCCTGAAC 
ACTCCACTGA GTGGCAGCAT GAAATCCATC CGCGGAGTGG ACTTCCAGTG CTTTGAGCAA 
GCCCGTAAAG CTGGACTACA TGGCACATTT AGAGCGTTTT TGTCTTCCCG ACTTCAAGAC 
CTGTACAGCA TTGTACGCAG AGCTGATCGA CAATCTGTGC AAATTGTTAA TCTTAGGGAT 
GAAGTGTTAT TTGACAACTG GGAGTCCCTG TTCTCTGGTT CTGAGGCTCA GATGAGGCCT 
GGTGCGAGTA TCTTCTCTTT TGATGGAAAA GATGTTGCAA CAGATCCTAC ATGGCCACAG 
AAGATGGTTT GGCATGGGTC TGACTCGAAG GGACGGCGGC TTACTGAAAG CTATTGCGAG 
ACCTGGCGCA CAGATGAAGG TGCAGTAACT GGTCAGGCAT CATCGCTGAC GAGCGGGAAA 
CTCTTAGAAC AGCGCCCACA GAGTTGCAAC AAGAACTTCA TTGTTTTGTG TATTGAGAAC 
AGCTTCATGA CCAATAACCG GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col18a1 ( XM_012970905.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826359.2 Xenopus tropicalis collagen type XVIII alpha 1 (col18a1), transcript variant X3, mRNA.

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CloneID	OXa66571
Clone ID Related Accession (Same CDS sequence)	XM_012970905.2
Accession Version	XM_012970905.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(XVIII) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970905.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCATTT CCATTCCACA AGTAACCTCT CTTTCCAGTG GCCTATCCCA TGATGTGTTA 
AATTCATCCC AGTTGAATGG GAATAACATA GGCAAACAGA GCACTTACAC CACCAATCAG 
GTTTATAAGA GCCCTGAGCT CCTGAAACAT TCTGGGATAG CTGCCACTCT TCATGGTGTT 
TCTGCACCTA ACAATTATGT CACTAGTCTA ACGGGACATC CCCATCCATC TGAAACTGAT 
TTACTAACCA GCACTAAATC TCCCATTCCA TCTTTTAGCC ATAATAGCTC CAGGACTGCT 
TTTATCTTGC CCAGATTGAC TCCATCAAAT TCTCGCTGCT TGCCTACCCC TACCAGCCTC 
TCATTCTGCA AAAGATTAGG AATTGACTCC TTTCTGCTCC CCAATTATCT GAACCATAGT 
ACTGTGCTGG AAATTCAGGC AGCTTTGCAT GATTGGGAGG GGCTGTTTAA ATCTCAGTGC 
CACCGCTACC TGGAATCGTT TTTCTGCTTG CTTCTGGCAC CAAGTTGTAA TGCTGGCACT 
GTGGCACCTC CGCTGGTGCC ATGCCGTCAG TTTTGTGAGG TGCTGCAGGA TGCCTGCTGG 
AATCTTCTGG AGGGATCACA TCTCCCAGTG TCATGTGACT CTCTTCCTGG GAAGAATTCT 
GGGGTTTCTT GTCTTTATAT TCATTTTTAT GCAGGTCCTA AGACACAAGT TTCCGCTTCT 
TCGGAGCTGA CAGACAACTT CAACCCAGAA GTTGGCCTCT TGCAGTTGAT TGGAGAACCT 
GTACCTGAAC AGATCACCAA GGTCTTTGGG CCCGACAAGA CTCCCGCCTA TGTATTTGGT 
AAAGAAGCCA ATACAGGCCA GGTGGCCCAA TACCATCTGC CCAACCCTAT GTATAGGGAC 
TTCTCTCTCC TCTTCCATGT CCAGCCAACC ACAGACAAGG CCGGCATCCT CTTTGCCATC 
ACAGATGCCT CTCAGTCTAT TGTGTATATC GGGGTGAAGC TTTCTGAAGT CAAGGATGGG 
AAGCAAGACA TCATCTTCTA CTATACAGAG CCTGGCTCAC AGGATTCCTA TGTAGCTGCC 
ACCTTCAATG TCCCATCACT GGTGAATCGT TGGTCTCGCT TTGCAATCAG TGTGGAGGAA 
GAAGAAGTTG TGCTGTTTAT GGATTGTGAT GAGCTGGAAA GGGTGCGCTT CGAGAGATCA 
CCTGATGAGA TGGAACTAGA AGATGGCTCT GGGCTCTTTG TTGCTCAAGC AGGTGGCGCT 
GATCCAGACA GATATCAGGG TGTAATAGCA GATCTAAAGA TCGTAGCCGA TCCAAGAGCC 
GCAGAACATC AGTGTGAGGA GGATGGAGAT GACTCTGATG GGATCTCTGG AGATTTTGGC 
AGTGGCATTG CGGACAATGA TTTGGTGACT TCTCCTCCTG TGATAATCAA AGTGCAGCCT 
CCTATAACTG TCCCTCCCAT ACAGAGGACC CCTGTGGAGA AAGAGATTGT TCAGACAGGT 
CAAGGCCAGC AAGGGGTAAA AGGAGAGAAG GGTGATCGTG GAGAGAAGGG TGAGAGGGGA 
CTGACTGGCC CTAAGGGAGA CGGTGGGAAT GCAAGTCCAA CTTCTGATTC CAAAGGAGAA 
AAGGGTCAGA AAGGAGAGGT CGGAGTGAAG GGAAGTGCAG GCTTTGGCTA TCCCGGGCCT 
AAAGGGCAGA AAGGAGATCC TGGAGACTTG GGACCAAGAG GACCACCAGG CCCACCTGGA 
CCATCTATAT TACAGAAAAG TGATGGAACT ACAGTGCAAC AGGTCACAGG ACCTCCAGGT 
CCTCAAGGCC CTGCGGGACC ACCAGGAAAA GAAGGGGAAC CAGGTGACCC TGGTGAAGAT 
GGAAAAACTG GCGAGGTTGG TCCCCAAGGA TTTCCAGGGA CCCCAGGAGA TTCTGGGCAG 
AAGGGAGACA AGGGTGATTC TGGTGTTGGT ATCAGAGGTG CTCCTGGGCC ACCAGGTCCG 
CCAGGCCCCC CTGGACCTTC TAGAACCGAC AAGCTGACAT TCATTGACAT GGAAGGTTCT 
GGATATGGTG GAGATTCGGA GCACATCAGG GGAGCCCCTG GTGTGCCTGG GCCCCCAGGT 
CCTCCAGGTG TACCAGGGTT ACCCGGAGTA CCCGGAACTT TTGGCATGAA CAACACAGGA 
TTGCCAGGTC CACCTGGATT GCCAGGTCTT CCCGGAAAGG AAGGAATTCC AGGTCCTCCT 
GGCCCCCCAG GACTCCCTGG AAAAGACGGG TTGTCTGGTA CACCTGGTGC TGCTGGATTA 
CGGGGTGAAG ATGGAGAGAT TGGTCTTCCA GGTGCTCCTG GAACAAAGGG TAGCACTGGT 
GAGCCAGGAC TACCTGGACC TCCTGGACAA TCTGGTTTAG CTGGACTTCC TGGACCAATG 
GGACCACGCG GGCTGCCAGG TCCTCCAGGA CCACCTGGGC CTGTGGGTCA TGGATCTGCA 
ATTGGCTTTG ATGACATGGA AGCCTCAGGG CTTGGATTTG GCTTGAGAGA AGGGACACAG 
GGGGTGCCTG GACTTCCCGG GGCAAAGGGA GAGCCAGGAC ATCCAGGGCT GCCAGGAGTG 
AAGGGAGATA GTGGTTTTCC AGGCCTTGAA GGAAGGCCGG GGTTGGATGG ATTCCCAGGT 
CCAGAGGGAT TAAAGGGTGA GAAAGGTGAA CGTGGTGAGC GGGGAGAATC TGGCCGTGAT 
GGTGTTGGTA TTCCAGGTCC ACCGGGACCT CCTGGTCAGG TGCTTTATTT ACCATCCGAT 
GACAAGACTA TTGTTGGTGC CATTCCTGGA CCAGAGGGCA GGCAAGGTGT AGCTGGACTT 
CCGGGCCCAA GGGGACCCAA AGGAGAGGCG GGCTTGAACG GTATCCCAGG AACCTTTGGA 
GTAAAGGGAG AAAAGGGAGA GCCTGGAATT ATTATGGGAC CAGATAGCAC CATTTACACT 
GATCTATCTA AAGGAGAGAA GGGTGACGAA GGACCAAGGG GACCAGCAGG AGCTGCGGGA 
CCATTTGGAC AAAAGGGAGA AATCGGGTTC CCAGGACGCC CTGGACGTCC TGGTATGAAT 
GGACTGAAAG GGGAGAAAGG AGAACCTAGT GGTGCAAGTG GTGGCTTTGG ACTGAGGGGG 
CCACCTGGTC CTCCTGGCCC ACCCGGTCCT CCTGGCAGTG TGGTGTATGG AGATCCAAAT 
AACCCAGGAG CCCAAGTATA TCCAGGTCAT AAAGGGGAGA GAGGATTTCC TGGACCCAAA 
GGTGATGTTG GTCCTGCTGG CCCACCAGGC TCTTTCCCAT ATGATTTAAG TACCTTTGCC 
TCGATTTTAA GGAGAGAAGC TGGACTCAAA GGAGAGAAGG GCGAATCTGG GGGTGGTTCC 
CATGGACAGG GGATCCCAGG GCCACCTGGA AACCCTGGAT ATCCAGGACT GCCGGGAGCT 
AAAGGAGACA GTGTTCAAGG TCCTCCAGGC CCAGCCGGCC CACCAGGACC TTCTGGAATC 
GGTTATGAAG GAAGGCAAGG TCCACCAGGG CCCCCAGGTC CTCCAGGCCT GCCAGGTTCC 
TCCTCTTATC CTGGGCCTCA CAGGCAAACC ATCAGCATAC CTGGACCTCC TGGCCCACCA 
GGCCCACCAG GGCTCCCAGG AACATCAGGA GGATTATCTG GGGCTACTGT GCTACAGACC 
TATCAGACTA TGATCAACTT AGCCCACAGG CTGCAGGAAG GGACAATTGT GCTGGTCCAG 
GAGAGGTCGG AACTGTATGT GCGTGTGAGA GAAGGCTTCC GAAAAATTCT TCTTGGTGAA 
TATACAACAT CCTCTGGCCC TGGACAGGAT AATGAAGTTG CTGCGGAGCA GGCCCCTGTA 
GTTCCATATC CACACCGTAA CAAAGATTCT GATTACCTCC AGTCTACAGA GGAGAACAGC 
AACAATCCAA ATGTGCCCTC AGGTGCCCGA CCCTGGAGCG ATCCTCGGTT ACCAGAACCA 
CCAAGAGTAA ACCCACCACC ACCCCCAGAC CATCACCACC ATGGAAACCC TCGTCCATTG 
GAACCATCCC CAGTGCACAC ACACCAAGAG TTTAATCCTG TGCTTCACCT TGTGGCCCTG 
AACACTCCAC TGAGTGGCAG CATGAAATCC ATCCGCGGAG TGGACTTCCA GTGCTTTGAG 
CAAGCCCGTA AAGCTGGACT ACATGGCACA TTTAGAGCGT TTTTGTCTTC CCGACTTCAA 
GACCTGTACA GCATTGTACG CAGAGCTGAT CGACAATCTG TGCAAATTGT TAATCTTAGG 
GATGAAGTGT TATTTGACAA CTGGGAGTCC CTGTTCTCTG GTTCTGAGGC TCAGATGAGG 
CCTGGTGCGA GTATCTTCTC TTTTGATGGA AAAGATGTTG CAACAGATCC TACATGGCCA 
CAGAAGATGG TTTGGCATGG GTCTGACTCG AAGGGACGGC GGCTTACTGA AAGCTATTGC 
GAGACCTGGC GCACAGATGA AGGTGCAGTA ACTGGTCAGG CATCATCGCT GACGAGCGGG 
AAACTCTTAG AACAGCGCCC ACAGAGTTGC AACAAGAACT TCATTGTTTT GTGTATTGAG 
AACAGCTTCA TGACCAATAA CCGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826359.2
CDS	168..4757
Translation	MRISIPQVTSLSSGLSHDVLNSSQLNGNNIGKQSTYTTNQVYKSPELLKHSGIAATLHGVSAPNNYVTSLTGHPHPSETDLLTSTKSPIPSFSHNSSRTAFILPRLTPSNSRCLPTPTSLSFCKRLGIDSFLLPNYLNHSTVLEIQAALHDWEGLFKSQCHRYLESFFCLLLAPSCNAGTVAPPLVPCRQFCEVLQDACWNLLEGSHLPVSCDSLPGKNSGVSCLYIHFYAGPKTQVSASSELTDNFNPEVGLLQLIGEPVPEQITKVFGPDKTPAYVFGKEANTGQVAQYHLPNPMYRDFSLLFHVQPTTDKAGILFAITDASQSIVYIGVKLSEVKDGKQDIIFYYTEPGSQDSYVAATFNVPSLVNRWSRFAISVEEEEVVLFMDCDELERVRFERSPDEMELEDGSGLFVAQAGGADPDRYQGVIADLKIVADPRAAEHQCEEDGDDSDGISGDFGSGIADNDLVTSPPVIIKVQPPITVPPIQRTPVEKEIVQTGQGQQGVKGEKGDRGEKGERGLTGPKGDGGNASPTSDSKGEKGQKGEVGVKGSAGFGYPGPKGQKGDPGDLGPRGPPGPPGPSILQKSDGTTVQQVTGPPGPQGPAGPPGKEGEPGDPGEDGKTGEVGPQGFPGTPGDSGQKGDKGDSGVGIRGAPGPPGPPGPPGPSRTDKLTFIDMEGSGYGGDSEHIRGAPGVPGPPGPPGVPGLPGVPGTFGMNNTGLPGPPGLPGLPGKEGIPGPPGPPGLPGKDGLSGTPGAAGLRGEDGEIGLPGAPGTKGSTGEPGLPGPPGQSGLAGLPGPMGPRGLPGPPGPPGPVGHGSAIGFDDMEASGLGFGLREGTQGVPGLPGAKGEPGHPGLPGVKGDSGFPGLEGRPGLDGFPGPEGLKGEKGERGERGESGRDGVGIPGPPGPPGQVLYLPSDDKTIVGAIPGPEGRQGVAGLPGPRGPKGEAGLNGIPGTFGVKGEKGEPGIIMGPDSTIYTDLSKGEKGDEGPRGPAGAAGPFGQKGEIGFPGRPGRPGMNGLKGEKGEPSGASGGFGLRGPPGPPGPPGPPGSVVYGDPNNPGAQVYPGHKGERGFPGPKGDVGPAGPPGSFPYDLSTFASILRREAGLKGEKGESGGGSHGQGIPGPPGNPGYPGLPGAKGDSVQGPPGPAGPPGPSGIGYEGRQGPPGPPGPPGLPGSSSYPGPHRQTISIPGPPGPPGPPGLPGTSGGLSGATVLQTYQTMINLAHRLQEGTIVLVQERSELYVRVREGFRKILLGEYTTSSGPGQDNEVAAEQAPVVPYPHRNKDSDYLQSTEENSNNPNVPSGARPWSDPRLPEPPRVNPPPPPDHHHHGNPRPLEPSPVHTHQEFNPVLHLVALNTPLSGSMKSIRGVDFQCFEQARKAGLHGTFRAFLSSRLQDLYSIVRRADRQSVQIVNLRDEVLFDNWESLFSGSEAQMRPGASIFSFDGKDVATDPTWPQKMVWHGSDSKGRRLTESYCETWRTDEGAVTGQASSLTSGKLLEQRPQSCNKNFIVLCIENSFMTNNRK

Target ORF information:

RefSeq Version	XM_012970905.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen type XVIII alpha 1 (col18a1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970905.2

ORF Insert Sequence:

ATGCGCATTT CCATTCCACA AGTAACCTCT CTTTCCAGTG GCCTATCCCA TGATGTGTTA 
AATTCATCCC AGTTGAATGG GAATAACATA GGCAAACAGA GCACTTACAC CACCAATCAG 
GTTTATAAGA GCCCTGAGCT CCTGAAACAT TCTGGGATAG CTGCCACTCT TCATGGTGTT 
TCTGCACCTA ACAATTATGT CACTAGTCTA ACGGGACATC CCCATCCATC TGAAACTGAT 
TTACTAACCA GCACTAAATC TCCCATTCCA TCTTTTAGCC ATAATAGCTC CAGGACTGCT 
TTTATCTTGC CCAGATTGAC TCCATCAAAT TCTCGCTGCT TGCCTACCCC TACCAGCCTC 
TCATTCTGCA AAAGATTAGG AATTGACTCC TTTCTGCTCC CCAATTATCT GAACCATAGT 
ACTGTGCTGG AAATTCAGGC AGCTTTGCAT GATTGGGAGG GGCTGTTTAA ATCTCAGTGC 
CACCGCTACC TGGAATCGTT TTTCTGCTTG CTTCTGGCAC CAAGTTGTAA TGCTGGCACT 
GTGGCACCTC CGCTGGTGCC ATGCCGTCAG TTTTGTGAGG TGCTGCAGGA TGCCTGCTGG 
AATCTTCTGG AGGGATCACA TCTCCCAGTG TCATGTGACT CTCTTCCTGG GAAGAATTCT 
GGGGTTTCTT GTCTTTATAT TCATTTTTAT GCAGGTCCTA AGACACAAGT TTCCGCTTCT 
TCGGAGCTGA CAGACAACTT CAACCCAGAA GTTGGCCTCT TGCAGTTGAT TGGAGAACCT 
GTACCTGAAC AGATCACCAA GGTCTTTGGG CCCGACAAGA CTCCCGCCTA TGTATTTGGT 
AAAGAAGCCA ATACAGGCCA GGTGGCCCAA TACCATCTGC CCAACCCTAT GTATAGGGAC 
TTCTCTCTCC TCTTCCATGT CCAGCCAACC ACAGACAAGG CCGGCATCCT CTTTGCCATC 
ACAGATGCCT CTCAGTCTAT TGTGTATATC GGGGTGAAGC TTTCTGAAGT CAAGGATGGG 
AAGCAAGACA TCATCTTCTA CTATACAGAG CCTGGCTCAC AGGATTCCTA TGTAGCTGCC 
ACCTTCAATG TCCCATCACT GGTGAATCGT TGGTCTCGCT TTGCAATCAG TGTGGAGGAA 
GAAGAAGTTG TGCTGTTTAT GGATTGTGAT GAGCTGGAAA GGGTGCGCTT CGAGAGATCA 
CCTGATGAGA TGGAACTAGA AGATGGCTCT GGGCTCTTTG TTGCTCAAGC AGGTGGCGCT 
GATCCAGACA GATATCAGGG TGTAATAGCA GATCTAAAGA TCGTAGCCGA TCCAAGAGCC 
GCAGAACATC AGTGTGAGGA GGATGGAGAT GACTCTGATG GGATCTCTGG AGATTTTGGC 
AGTGGCATTG CGGACAATGA TTTGGTGACT TCTCCTCCTG TGATAATCAA AGTGCAGCCT 
CCTATAACTG TCCCTCCCAT ACAGAGGACC CCTGTGGAGA AAGAGATTGT TCAGACAGGT 
CAAGGCCAGC AAGGGGTAAA AGGAGAGAAG GGTGATCGTG GAGAGAAGGG TGAGAGGGGA 
CTGACTGGCC CTAAGGGAGA CGGTGGGAAT GCAAGTCCAA CTTCTGATTC CAAAGGAGAA 
AAGGGTCAGA AAGGAGAGGT CGGAGTGAAG GGAAGTGCAG GCTTTGGCTA TCCCGGGCCT 
AAAGGGCAGA AAGGAGATCC TGGAGACTTG GGACCAAGAG GACCACCAGG CCCACCTGGA 
CCATCTATAT TACAGAAAAG TGATGGAACT ACAGTGCAAC AGGTCACAGG ACCTCCAGGT 
CCTCAAGGCC CTGCGGGACC ACCAGGAAAA GAAGGGGAAC CAGGTGACCC TGGTGAAGAT 
GGAAAAACTG GCGAGGTTGG TCCCCAAGGA TTTCCAGGGA CCCCAGGAGA TTCTGGGCAG 
AAGGGAGACA AGGGTGATTC TGGTGTTGGT ATCAGAGGTG CTCCTGGGCC ACCAGGTCCG 
CCAGGCCCCC CTGGACCTTC TAGAACCGAC AAGCTGACAT TCATTGACAT GGAAGGTTCT 
GGATATGGTG GAGATTCGGA GCACATCAGG GGAGCCCCTG GTGTGCCTGG GCCCCCAGGT 
CCTCCAGGTG TACCAGGGTT ACCCGGAGTA CCCGGAACTT TTGGCATGAA CAACACAGGA 
TTGCCAGGTC CACCTGGATT GCCAGGTCTT CCCGGAAAGG AAGGAATTCC AGGTCCTCCT 
GGCCCCCCAG GACTCCCTGG AAAAGACGGG TTGTCTGGTA CACCTGGTGC TGCTGGATTA 
CGGGGTGAAG ATGGAGAGAT TGGTCTTCCA GGTGCTCCTG GAACAAAGGG TAGCACTGGT 
GAGCCAGGAC TACCTGGACC TCCTGGACAA TCTGGTTTAG CTGGACTTCC TGGACCAATG 
GGACCACGCG GGCTGCCAGG TCCTCCAGGA CCACCTGGGC CTGTGGGTCA TGGATCTGCA 
ATTGGCTTTG ATGACATGGA AGCCTCAGGG CTTGGATTTG GCTTGAGAGA AGGGACACAG 
GGGGTGCCTG GACTTCCCGG GGCAAAGGGA GAGCCAGGAC ATCCAGGGCT GCCAGGAGTG 
AAGGGAGATA GTGGTTTTCC AGGCCTTGAA GGAAGGCCGG GGTTGGATGG ATTCCCAGGT 
CCAGAGGGAT TAAAGGGTGA GAAAGGTGAA CGTGGTGAGC GGGGAGAATC TGGCCGTGAT 
GGTGTTGGTA TTCCAGGTCC ACCGGGACCT CCTGGTCAGG TGCTTTATTT ACCATCCGAT 
GACAAGACTA TTGTTGGTGC CATTCCTGGA CCAGAGGGCA GGCAAGGTGT AGCTGGACTT 
CCGGGCCCAA GGGGACCCAA AGGAGAGGCG GGCTTGAACG GTATCCCAGG AACCTTTGGA 
GTAAAGGGAG AAAAGGGAGA GCCTGGAATT ATTATGGGAC CAGATAGCAC CATTTACACT 
GATCTATCTA AAGGAGAGAA GGGTGACGAA GGACCAAGGG GACCAGCAGG AGCTGCGGGA 
CCATTTGGAC AAAAGGGAGA AATCGGGTTC CCAGGACGCC CTGGACGTCC TGGTATGAAT 
GGACTGAAAG GGGAGAAAGG AGAACCTAGT GGTGCAAGTG GTGGCTTTGG ACTGAGGGGG 
CCACCTGGTC CTCCTGGCCC ACCCGGTCCT CCTGGCAGTG TGGTGTATGG AGATCCAAAT 
AACCCAGGAG CCCAAGTATA TCCAGGTCAT AAAGGGGAGA GAGGATTTCC TGGACCCAAA 
GGTGATGTTG GTCCTGCTGG CCCACCAGGC TCTTTCCCAT ATGATTTAAG TACCTTTGCC 
TCGATTTTAA GGAGAGAAGC TGGACTCAAA GGAGAGAAGG GCGAATCTGG GGGTGGTTCC 
CATGGACAGG GGATCCCAGG GCCACCTGGA AACCCTGGAT ATCCAGGACT GCCGGGAGCT 
AAAGGAGACA GTGTTCAAGG TCCTCCAGGC CCAGCCGGCC CACCAGGACC TTCTGGAATC 
GGTTATGAAG GAAGGCAAGG TCCACCAGGG CCCCCAGGTC CTCCAGGCCT GCCAGGTTCC 
TCCTCTTATC CTGGGCCTCA CAGGCAAACC ATCAGCATAC CTGGACCTCC TGGCCCACCA 
GGCCCACCAG GGCTCCCAGG AACATCAGGA GGATTATCTG GGGCTACTGT GCTACAGACC 
TATCAGACTA TGATCAACTT AGCCCACAGG CTGCAGGAAG GGACAATTGT GCTGGTCCAG 
GAGAGGTCGG AACTGTATGT GCGTGTGAGA GAAGGCTTCC GAAAAATTCT TCTTGGTGAA 
TATACAACAT CCTCTGGCCC TGGACAGGAT AATGAAGTTG CTGCGGAGCA GGCCCCTGTA 
GTTCCATATC CACACCGTAA CAAAGATTCT GATTACCTCC AGTCTACAGA GGAGAACAGC 
AACAATCCAA ATGTGCCCTC AGGTGCCCGA CCCTGGAGCG ATCCTCGGTT ACCAGAACCA 
CCAAGAGTAA ACCCACCACC ACCCCCAGAC CATCACCACC ATGGAAACCC TCGTCCATTG 
GAACCATCCC CAGTGCACAC ACACCAAGAG TTTAATCCTG TGCTTCACCT TGTGGCCCTG 
AACACTCCAC TGAGTGGCAG CATGAAATCC ATCCGCGGAG TGGACTTCCA GTGCTTTGAG 
CAAGCCCGTA AAGCTGGACT ACATGGCACA TTTAGAGCGT TTTTGTCTTC CCGACTTCAA 
GACCTGTACA GCATTGTACG CAGAGCTGAT CGACAATCTG TGCAAATTGT TAATCTTAGG 
GATGAAGTGT TATTTGACAA CTGGGAGTCC CTGTTCTCTG GTTCTGAGGC TCAGATGAGG 
CCTGGTGCGA GTATCTTCTC TTTTGATGGA AAAGATGTTG CAACAGATCC TACATGGCCA 
CAGAAGATGG TTTGGCATGG GTCTGACTCG AAGGGACGGC GGCTTACTGA AAGCTATTGC 
GAGACCTGGC GCACAGATGA AGGTGCAGTA ACTGGTCAGG CATCATCGCT GACGAGCGGG 
AAACTCTTAG AACAGCGCCC ACAGAGTTGC AACAAGAACT TCATTGTTTT GTGTATTGAG 
AACAGCTTCA TGACCAATAA CCGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col18a1 ( XM_012970907.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826361.1 Xenopus tropicalis collagen, type XVIII, alpha 1 (col18a1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa21931
Clone ID Related Accession (Same CDS sequence)	XM_012970907.2
Accession Version	XM_012970907.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(XVIII) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970907.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCTCA GGTGTACGTG GGTCCTGTTG CTCTACGCAT CGTATCATGT TCTTCTGGTC 
TCCTCCCAAG GCAAAGACAA CTTCAACCCA GAAGTTGGCC TCTTGCAGTT GATTGGAGAA 
CCTGTACCTG AACAGATCAC CAAGGTCTTT GGGCCCGACA AGACTCCCGC CTATGTATTT 
GGTAAAGAAG CCAATACAGG CCAGGTGGCC CAATACCATC TGCCCAACCC TATGTATAGG 
GACTTCTCTC TCCTCTTCCA TGTCCAGCCA ACCACAGACA AGGCCGGCAT CCTCTTTGCC 
ATCACAGATG CCTCTCAGTC TATTGTGTAT ATCGGGGTGA AGCTTTCTGA AGTCAAGGAT 
GGGAAGCAAG ACATCATCTT CTACTATACA GAGCCTGGCT CACAGGATTC CTATGTAGCT 
GCCACCTTCA ATGTCCCATC ACTGGTGAAT CGTTGGTCTC GCTTTGCAAT CAGTGTGGAG 
GAAGAAGAAG TTGTGCTGTT TATGGATTGC GATGAGCTGG AAAGGGTGCG CTTCGAGAGA 
TCACCTGATG AGATGGAACT AGAAGATGGC TCTGGGCTCT TTGTTGCTCA AGCAGGTGGC 
GCTGATCCAG ACAGATATCA GGGTGTAATA GCAGATCTAA AGATCGTAGC CGATCCAAGA 
GCCGCAGAAC ATCAGTGTGA GGAGGATGGA GATGACTCTG ATGGGATCTC TGGAGATTTT 
GGCAGTGGCA TTGCGGACAA TGATTTGGTG ACTTCTCCTC CTGTGATAAT CAAAGTGCAG 
CCTCCTATAA CTGTCCCTCC CATACAGAGG ACCCCTGTGG AGAAAGAGAT TGTTCAGACA 
GGTCAAGGCC AGCAAGGGGT AAAAGGAGAG AAGGGTGATC GTGGAGAGAA GGGTGAGAGG 
GGACTGACTG GCCCTAAGGG AGACGGTGGG AATGCAAGTC CAACTTCTGA TTCCAAAGGA 
GAAAAGGGTC AGAAAGGAGA GGTCGGAGTG AAGGGAAGTG CAGGCTTTGG CTATCCCGGG 
CCTAAAGGGC AGAAAGGAGA TCCTGGAGAC TTGGGACCAA GAGGACCACC AGGCCCACCT 
GGACCATCTA TATTACAGAA AAGTGATGGA ACTACAGTGC AACAGGTCAC AGGACCTCCA 
GGTCCTCAAG GCCCTGCGGG ACCACCAGGA AAAGAAGGGG AACCAGGTGA CCCTGGTGAA 
GATGGAAAAA CTGGCGAGGT TGGTCCCCAA GGATTTCCAG GGACCCCAGG AGATTCTGGG 
CAGAAGGGAG ACAAGGGTGA TTCTGGTGTT GGTATCAGAG GTGCTCCTGG GCCACCAGGT 
CCGCCAGGCC CCCCTGGACC TTCTAGAACC GACAAGCTGA CATTCATTGA CATGGAAGGT 
TCTGGATATG GTGGAGATTC GGAGCACATC AGGGGAGCCC CTGGTGTGCC TGGGCCCCCA 
GGTCCTCCAG GTGTACCAGG GTTACCCGGA GTACCCGGAA CTTTTGGCAT GAACAACACA 
GGATTGCCAG GTCCACCTGG ATTGCCAGGT CTTCCCGGAA AGGAAGGAAT TCCAGGTCCT 
CCTGGCCCCC CAGGACTCCC TGGAAAAGAC GGGTTGTCTG GTACACCTGG TGCTGCTGGA 
TTACGGGGTG AAGATGGAGA GATTGGTCTT CCAGGTGCTC CTGGAACAAA GGGTAGCACT 
GGTGAGCCAG GACTACCTGG ACCTCCTGGA CAATCTGGTT TAGCTGGACT TCCTGGACCA 
ATGGGACCAC GCGGGCTGCC AGGTCCTCCA GGACCACCTG GGCCTGTGGG TCATGGATCT 
GCAATTGGCT TTGATGACAT GGAAGCCTCA GGGCTTGGAT TTGGCTTGAG AGAAGGGACA 
CAGGGGGTGC CTGGACTTCC CGGGGCAAAG GGAGAGCCAG GACATCCAGG GCTGCCAGGA 
GTGAAGGGAG ATAGTGGTTT TCCAGGCCTT GAAGGAAGGC CGGGGTTGGA TGGATTCCCA 
GGTCCAGAGG GATTAAAGGG TGAGAAAGGT GAACGTGGTG AGCGGGGAGA ATCTGGCCGT 
GATGGTGTTG GTATTCCAGG TCCACCGGGA CCTCCTGGTC AGGTGCTTTA TTTACCATCC 
GATGACAAGA CTATTGTTGG TGCCATTCCT GGACCAGAGG GCCCAAGGGG ACCCAAAGGA 
GAGGCGGGCT TGAACGGTAT CCCAGGAACC TTTGGAGTAA AGGGAGAGAA GGGAGAGCCT 
GGAATTATTA TGGGACCAGA TAGCACCATT TACACTGATT CATCTAAAGG AGAGAAGGGT 
GACGAAGGAC CAAGGGGACC AGCAGGAGCT GCGGGACCAT TTGGACAAAA GGGAGAAATC 
GGGTTCCCAG GACGCCCTGG ACGTCCTGGT ATGAATGGAC TGAAAGGGGA GAAAGGAGAA 
CCTAGTGGTG CAAGTGGTGG CTTTGGACTG AGGGGGCCAC CTGGTCCTCC TGGCCCACCC 
GGTCCTCCTG GCAGTGTGGT GTATGGCGAT CCAAATAACC CAGGAGCCCA AGTATATCCA 
GGTCATAAAG GGGAGAGAGG ATTTCCTGGA CCCAAAGGTG ATGTTGGTCC TGCTGGCCCA 
CCAGGCTCTT TCCCATATGA TTTAAGTACC TTTGCCTCGA TTTTAAGGAG AGAAGCTGGA 
CTCAAAGGAG AGAAGGGCGA ATCTGGGGGT GGTTCCCATG GACAGGGGAT CCCAGGGCCA 
CCTGGAAACC CTGGATATCC AGGACTGCCG GGAGCTAAAG GAGACAGTGT TCAAGGTCCT 
CCAGGCCCAG CCGGCCCACC AGGACCTTCT GGAATCGGTT ATGAAGGAAG GCAAGGTCCA 
CCAGGGCCCC CAGGTCCTCC AGGCCTGCCA GGTTCCTCCT CTTATCCTGG GCCTCACAGG 
CAAACCATCA GCATACCTGG ACCTCCTGGC CCACCAGGCC CACCAGGGCT CCCAGGAACA 
TCAGGAGGAT TATCTGGGGC TACTGTGCTA CAGACCTATC AGACTATGAT CAACTTAGCC 
CACAGGCTGC AGGAAGGGAC AATTGTGCTG GTCCAGGAGA GGTCGGAACT GTATGTGCGT 
GTGAGAGAAG GCTTCCGAAA AATTCTTCTT GGTGAATATA CAACATCCTC TGGCCCTGGA 
CAGGATAATG AAGTTGCTGC GGAGCAGGCC CCTGTAGTTC CATATCCACA CCGTAACAAA 
GATTCTGATT ACCTCCAGTC TACAGAGGAG AACAGCAACA ATCCAAATGT GCCCTCAGGT 
GCCCGACCCT GGAGCGATCC TCGGTTACCA GAACCACCAA GAGTAAACCC ACCACCACCC 
CCAGACCATC ACCACCATGG AAACCCTCGT CCATTGGAAC CATCCCCAGT GCACACACAC 
CAAGAGTTTA ATCCTGTGCT TCACCTTGTG GCCCTGAACA CTCCACTGAG TGGCAGCATG 
AAATCCATCC GCGGAGTGGA CTTCCAGTGC TTTGAGCAAG CCCGTAAAGC TGGACTACAT 
GGCACATTTA GAGCGTTTTT GTCTTCCCGA CTTCAAGACC TGTACAGCAT TGTACGCAGA 
GCTGATCGAC AATCTGTGCA AATTGTTAAT CTTAGGGATG AAGTGTTATT TGACAACTGG 
GAGTCCCTGT TCTCTGGTTC TGAGGCTCAG ATGAGGCCTG GTGCGAGTAT CTTCTCTTTT 
GATGGAAAAG ATGTTGCAAC AGATCCTACA TGGCCACAGA AGATGGTTTG GCATGGGTCT 
GACTCGAAGG GACGGCGGCT TACTGAAAGC TATTGCGAGA CCTGGCGCAC AGATGAAGGT 
GCAGTAACTG GTCAGGCATC ATCGCTGACG AGTGGGAAAC TCTTAGAACA GCGCCCACAG 
AGTTGCAACA AGAACTTCAT TGTTTTGTGT ATTGAGAACA GCTTCATGAC CAATAACCGG 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826361.1
CDS	178..4083
Translation	MILRCTWVLLLYASYHVLLVSSQGKDNFNPEVGLLQLIGEPVPEQITKVFGPDKTPAYVFGKEANTGQVAQYHLPNPMYRDFSLLFHVQPTTDKAGILFAITDASQSIVYIGVKLSEVKDGKQDIIFYYTEPGSQDSYVAATFNVPSLVNRWSRFAISVEEEEVVLFMDCDELERVRFERSPDEMELEDGSGLFVAQAGGADPDRYQGVIADLKIVADPRAAEHQCEEDGDDSDGISGDFGSGIADNDLVTSPPVIIKVQPPITVPPIQRTPVEKEIVQTGQGQQGVKGEKGDRGEKGERGLTGPKGDGGNASPTSDSKGEKGQKGEVGVKGSAGFGYPGPKGQKGDPGDLGPRGPPGPPGPSILQKSDGTTVQQVTGPPGPQGPAGPPGKEGEPGDPGEDGKTGEVGPQGFPGTPGDSGQKGDKGDSGVGIRGAPGPPGPPGPPGPSRTDKLTFIDMEGSGYGGDSEHIRGAPGVPGPPGPPGVPGLPGVPGTFGMNNTGLPGPPGLPGLPGKEGIPGPPGPPGLPGKDGLSGTPGAAGLRGEDGEIGLPGAPGTKGSTGEPGLPGPPGQSGLAGLPGPMGPRGLPGPPGPPGPVGHGSAIGFDDMEASGLGFGLREGTQGVPGLPGAKGEPGHPGLPGVKGDSGFPGLEGRPGLDGFPGPEGLKGEKGERGERGESGRDGVGIPGPPGPPGQVLYLPSDDKTIVGAIPGPEGPRGPKGEAGLNGIPGTFGVKGEKGEPGIIMGPDSTIYTDSSKGEKGDEGPRGPAGAAGPFGQKGEIGFPGRPGRPGMNGLKGEKGEPSGASGGFGLRGPPGPPGPPGPPGSVVYGDPNNPGAQVYPGHKGERGFPGPKGDVGPAGPPGSFPYDLSTFASILRREAGLKGEKGESGGGSHGQGIPGPPGNPGYPGLPGAKGDSVQGPPGPAGPPGPSGIGYEGRQGPPGPPGPPGLPGSSSYPGPHRQTISIPGPPGPPGPPGLPGTSGGLSGATVLQTYQTMINLAHRLQEGTIVLVQERSELYVRVREGFRKILLGEYTTSSGPGQDNEVAAEQAPVVPYPHRNKDSDYLQSTEENSNNPNVPSGARPWSDPRLPEPPRVNPPPPPDHHHHGNPRPLEPSPVHTHQEFNPVLHLVALNTPLSGSMKSIRGVDFQCFEQARKAGLHGTFRAFLSSRLQDLYSIVRRADRQSVQIVNLRDEVLFDNWESLFSGSEAQMRPGASIFSFDGKDVATDPTWPQKMVWHGSDSKGRRLTESYCETWRTDEGAVTGQASSLTSGKLLEQRPQSCNKNFIVLCIENSFMTNNRK

Target ORF information:

RefSeq Version	XM_012970907.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type XVIII, alpha 1 (col18a1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970907.2

ORF Insert Sequence:

ATGATTCTCA GGTGTACGTG GGTCCTGTTG CTCTACGCAT CGTATCATGT TCTTCTGGTC 
TCCTCCCAAG GCAAAGACAA CTTCAACCCA GAAGTTGGCC TCTTGCAGTT GATTGGAGAA 
CCTGTACCTG AACAGATCAC CAAGGTCTTT GGGCCCGACA AGACTCCCGC CTATGTATTT 
GGTAAAGAAG CCAATACAGG CCAGGTGGCC CAATACCATC TGCCCAACCC TATGTATAGG 
GACTTCTCTC TCCTCTTCCA TGTCCAGCCA ACCACAGACA AGGCCGGCAT CCTCTTTGCC 
ATCACAGATG CCTCTCAGTC TATTGTGTAT ATCGGGGTGA AGCTTTCTGA AGTCAAGGAT 
GGGAAGCAAG ACATCATCTT CTACTATACA GAGCCTGGCT CACAGGATTC CTATGTAGCT 
GCCACCTTCA ATGTCCCATC ACTGGTGAAT CGTTGGTCTC GCTTTGCAAT CAGTGTGGAG 
GAAGAAGAAG TTGTGCTGTT TATGGATTGC GATGAGCTGG AAAGGGTGCG CTTCGAGAGA 
TCACCTGATG AGATGGAACT AGAAGATGGC TCTGGGCTCT TTGTTGCTCA AGCAGGTGGC 
GCTGATCCAG ACAGATATCA GGGTGTAATA GCAGATCTAA AGATCGTAGC CGATCCAAGA 
GCCGCAGAAC ATCAGTGTGA GGAGGATGGA GATGACTCTG ATGGGATCTC TGGAGATTTT 
GGCAGTGGCA TTGCGGACAA TGATTTGGTG ACTTCTCCTC CTGTGATAAT CAAAGTGCAG 
CCTCCTATAA CTGTCCCTCC CATACAGAGG ACCCCTGTGG AGAAAGAGAT TGTTCAGACA 
GGTCAAGGCC AGCAAGGGGT AAAAGGAGAG AAGGGTGATC GTGGAGAGAA GGGTGAGAGG 
GGACTGACTG GCCCTAAGGG AGACGGTGGG AATGCAAGTC CAACTTCTGA TTCCAAAGGA 
GAAAAGGGTC AGAAAGGAGA GGTCGGAGTG AAGGGAAGTG CAGGCTTTGG CTATCCCGGG 
CCTAAAGGGC AGAAAGGAGA TCCTGGAGAC TTGGGACCAA GAGGACCACC AGGCCCACCT 
GGACCATCTA TATTACAGAA AAGTGATGGA ACTACAGTGC AACAGGTCAC AGGACCTCCA 
GGTCCTCAAG GCCCTGCGGG ACCACCAGGA AAAGAAGGGG AACCAGGTGA CCCTGGTGAA 
GATGGAAAAA CTGGCGAGGT TGGTCCCCAA GGATTTCCAG GGACCCCAGG AGATTCTGGG 
CAGAAGGGAG ACAAGGGTGA TTCTGGTGTT GGTATCAGAG GTGCTCCTGG GCCACCAGGT 
CCGCCAGGCC CCCCTGGACC TTCTAGAACC GACAAGCTGA CATTCATTGA CATGGAAGGT 
TCTGGATATG GTGGAGATTC GGAGCACATC AGGGGAGCCC CTGGTGTGCC TGGGCCCCCA 
GGTCCTCCAG GTGTACCAGG GTTACCCGGA GTACCCGGAA CTTTTGGCAT GAACAACACA 
GGATTGCCAG GTCCACCTGG ATTGCCAGGT CTTCCCGGAA AGGAAGGAAT TCCAGGTCCT 
CCTGGCCCCC CAGGACTCCC TGGAAAAGAC GGGTTGTCTG GTACACCTGG TGCTGCTGGA 
TTACGGGGTG AAGATGGAGA GATTGGTCTT CCAGGTGCTC CTGGAACAAA GGGTAGCACT 
GGTGAGCCAG GACTACCTGG ACCTCCTGGA CAATCTGGTT TAGCTGGACT TCCTGGACCA 
ATGGGACCAC GCGGGCTGCC AGGTCCTCCA GGACCACCTG GGCCTGTGGG TCATGGATCT 
GCAATTGGCT TTGATGACAT GGAAGCCTCA GGGCTTGGAT TTGGCTTGAG AGAAGGGACA 
CAGGGGGTGC CTGGACTTCC CGGGGCAAAG GGAGAGCCAG GACATCCAGG GCTGCCAGGA 
GTGAAGGGAG ATAGTGGTTT TCCAGGCCTT GAAGGAAGGC CGGGGTTGGA TGGATTCCCA 
GGTCCAGAGG GATTAAAGGG TGAGAAAGGT GAACGTGGTG AGCGGGGAGA ATCTGGCCGT 
GATGGTGTTG GTATTCCAGG TCCACCGGGA CCTCCTGGTC AGGTGCTTTA TTTACCATCC 
GATGACAAGA CTATTGTTGG TGCCATTCCT GGACCAGAGG GCCCAAGGGG ACCCAAAGGA 
GAGGCGGGCT TGAACGGTAT CCCAGGAACC TTTGGAGTAA AGGGAGAGAA GGGAGAGCCT 
GGAATTATTA TGGGACCAGA TAGCACCATT TACACTGATT CATCTAAAGG AGAGAAGGGT 
GACGAAGGAC CAAGGGGACC AGCAGGAGCT GCGGGACCAT TTGGACAAAA GGGAGAAATC 
GGGTTCCCAG GACGCCCTGG ACGTCCTGGT ATGAATGGAC TGAAAGGGGA GAAAGGAGAA 
CCTAGTGGTG CAAGTGGTGG CTTTGGACTG AGGGGGCCAC CTGGTCCTCC TGGCCCACCC 
GGTCCTCCTG GCAGTGTGGT GTATGGCGAT CCAAATAACC CAGGAGCCCA AGTATATCCA 
GGTCATAAAG GGGAGAGAGG ATTTCCTGGA CCCAAAGGTG ATGTTGGTCC TGCTGGCCCA 
CCAGGCTCTT TCCCATATGA TTTAAGTACC TTTGCCTCGA TTTTAAGGAG AGAAGCTGGA 
CTCAAAGGAG AGAAGGGCGA ATCTGGGGGT GGTTCCCATG GACAGGGGAT CCCAGGGCCA 
CCTGGAAACC CTGGATATCC AGGACTGCCG GGAGCTAAAG GAGACAGTGT TCAAGGTCCT 
CCAGGCCCAG CCGGCCCACC AGGACCTTCT GGAATCGGTT ATGAAGGAAG GCAAGGTCCA 
CCAGGGCCCC CAGGTCCTCC AGGCCTGCCA GGTTCCTCCT CTTATCCTGG GCCTCACAGG 
CAAACCATCA GCATACCTGG ACCTCCTGGC CCACCAGGCC CACCAGGGCT CCCAGGAACA 
TCAGGAGGAT TATCTGGGGC TACTGTGCTA CAGACCTATC AGACTATGAT CAACTTAGCC 
CACAGGCTGC AGGAAGGGAC AATTGTGCTG GTCCAGGAGA GGTCGGAACT GTATGTGCGT 
GTGAGAGAAG GCTTCCGAAA AATTCTTCTT GGTGAATATA CAACATCCTC TGGCCCTGGA 
CAGGATAATG AAGTTGCTGC GGAGCAGGCC CCTGTAGTTC CATATCCACA CCGTAACAAA 
GATTCTGATT ACCTCCAGTC TACAGAGGAG AACAGCAACA ATCCAAATGT GCCCTCAGGT 
GCCCGACCCT GGAGCGATCC TCGGTTACCA GAACCACCAA GAGTAAACCC ACCACCACCC 
CCAGACCATC ACCACCATGG AAACCCTCGT CCATTGGAAC CATCCCCAGT GCACACACAC 
CAAGAGTTTA ATCCTGTGCT TCACCTTGTG GCCCTGAACA CTCCACTGAG TGGCAGCATG 
AAATCCATCC GCGGAGTGGA CTTCCAGTGC TTTGAGCAAG CCCGTAAAGC TGGACTACAT 
GGCACATTTA GAGCGTTTTT GTCTTCCCGA CTTCAAGACC TGTACAGCAT TGTACGCAGA 
GCTGATCGAC AATCTGTGCA AATTGTTAAT CTTAGGGATG AAGTGTTATT TGACAACTGG 
GAGTCCCTGT TCTCTGGTTC TGAGGCTCAG ATGAGGCCTG GTGCGAGTAT CTTCTCTTTT 
GATGGAAAAG ATGTTGCAAC AGATCCTACA TGGCCACAGA AGATGGTTTG GCATGGGTCT 
GACTCGAAGG GACGGCGGCT TACTGAAAGC TATTGCGAGA CCTGGCGCAC AGATGAAGGT 
GCAGTAACTG GTCAGGCATC ATCGCTGACG AGTGGGAAAC TCTTAGAACA GCGCCCACAG 
AGTTGCAACA AGAACTTCAT TGTTTTGTGT ATTGAGAACA GCTTCATGAC CAATAACCGG 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col18a1 ( XM_012970903.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826357.1 Xenopus tropicalis collagen, type XVIII, alpha 1 (col18a1), transcript variant X1, mRNA.

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CloneID	OXa26097
Clone ID Related Accession (Same CDS sequence)	XM_012970903.2
Accession Version	XM_012970903.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(XVIII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970903.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCAG CGCGGCTTAG CCTTGGGGTC ACAGTGCTTG TGCTCCTCTT ACCTGCTCTA 
TCCAATGGCC AGTTTTGGTC CTGGTTTACA GGGAGCAAAG AGACAACTGC GAGCCCCACA 
ACTCAACGTG TAACTCCAGG AACATCCTCA GTTCATAGTA CTGCTGTGCA GCATCACTCA 
CCTGATGCCT CGCCAACCAC ATTGCACCAT ATACCGGATG CACTACCCTC AGCGGAAAGT 
ACGTCAGCAC CTGCAGTGCA TCAGCCACCT GAAGAAGATA ACCTGCCTGA GACAACACCC 
TCTCACCTAG AGGCAGCCTC TGTGCCATCT TCAGAAAAAC CCACACCTTT TACAGAACAG 
TCACCCACAG CATCCTCCTC AGCCATACTC ATTCCCAGCA CAGTCATCCT AACCACAGAG 
GGCAAGAATG ACAGTACTGA GACAGCAGGA GTGACCAGAC CCCTCCAAAC GACAGAGTCC 
CCTACTACTT CCAGGAGGCA GGAGGAAAAG CAAATATGGA ACATATTCAG TGTGCAGAAA 
GGAAAGGAGA ACGTAGCGCC TGGGACTTTG GCCACAACAC CTGTAGCGAA GGATGAAACC 
AGTGCACCTT CTGTGTCAGA GGTTCCAATA TCTCAGAAGC CACAAAGTGA ACAAAACATT 
GCTGGTGTAG GGGCCGAGAT CTTAAATGTA GCAGAAGGAA TTAGGAACTT TGTGCATGGC 
TGGGAAGATA ACATTGCCAA CAGCACAAAG CAGACTCCTA CTCCTGACAC GGAGATGGTG 
ACTGCCACAA CAGCCCCAAG CCTTACTGTA AATGACACTG GCACCTTAAG CTACAACAAG 
ACAGAACCAA GCGAGAACAC TATGGCAAAC CATACGGAAG GTCCGGTGTC CGAGCCCCAT 
AAACTGATCT CATCCCACAA CAAGCCACTT CTACCAGACA ACTTCAACCC AGAAGTTGGC 
CTCTTGCAGT TGATTGGAGA ACCTGTACCT GAACAGATCA CCAAGGTCTT TGGGCCCGAC 
AAGACTCCCG CCTATGTATT TGGTAAAGAA GCCAATACAG GCCAGGTGGC CCAATACCAT 
CTGCCCAACC CTATGTATAG GGACTTCTCT CTCCTCTTCC ATGTCCAGCC AACCACAGAC 
AAGGCCGGCA TCCTCTTTGC CATCACAGAT GCCTCTCAGT CTATTGTGTA TATCGGGGTG 
AAGCTTTCTG AAGTCAAGGA TGGGAAGCAA GACATCATCT TCTACTATAC AGAGCCTGGC 
TCACAGGATT CCTATGTAGC TGCCACCTTC AATGTCCCAT CACTGGTGAA TCGTTGGTCT 
CGCTTTGCAA TCAGTGTGGA GGAAGAAGAA GTTGTGCTGT TTATGGATTG CGATGAGCTG 
GAAAGGGTGC GCTTCGAGAG ATCACCTGAT GAGATGGAAC TAGAAGATGG CTCTGGGCTC 
TTTGTTGCTC AAGCAGGTGG CGCTGATCCA GACAGATATC AGGGTGTAAT AGCAGATCTA 
AAGATCGTAG CCGATCCAAG AGCCGCAGAA CATCAGTGTG AGGAGGATGG AGATGACTCT 
GATGGGATCT CTGGAGATTT TGGCAGTGGC ATTGCGGACA ATGATTTGGT GACTTCTCCT 
CCTGTGATAA TCAAAGTGCA GCCTCCTATA ACTGTCCCTC CCATACAGAG GACCCCTGTG 
GAGAAAGAGA TTGTTCAGAC AGGTCAAGGC CAGCAAGGGG TAAAAGGAGA GAAGGGTGAT 
CGTGGAGAGA AGGGTGAGAG GGGACTGACT GGCCCTAAGG GAGACGGTGG GAATGCAAGT 
CCAACTTCTG ATTCCAAAGG AGAAAAGGGT CAGAAAGGAG AGGTCGGAGT GAAGGGAAGT 
GCAGGCTTTG GCTATCCCGG GCCTAAAGGG CAGAAAGGAG ATCCTGGAGA CTTGGGACCA 
AGAGGACCAC CAGGCCCACC TGGACCATCT ATATTACAGA AAAGTGATGG AACTACAGTG 
CAACAGGTCA CAGGACCTCC AGGTCCTCAA GGCCCTGCGG GACCACCAGG AAAAGAAGGG 
GAACCAGGTG ACCCTGGTGA AGATGGAAAA ACTGGCGAGG TTGGTCCCCA AGGATTTCCA 
GGGACCCCAG GAGATTCTGG GCAGAAGGGA GACAAGGGTG ATTCTGGTGT TGGTATCAGA 
GGTGCTCCTG GGCCACCAGG TCCGCCAGGC CCCCCTGGAC CTTCTAGAAC CGACAAGCTG 
ACATTCATTG ACATGGAAGG TTCTGGATAT GGTGGAGATT CGGAGCACAT CAGGGGAGCC 
CCTGGTGTGC CTGGGCCCCC AGGTCCTCCA GGTGTACCAG GGTTACCCGG AGTACCCGGA 
ACTTTTGGCA TGAACAACAC AGGATTGCCA GGTCCACCTG GATTGCCAGG TCTTCCCGGA 
AAGGAAGGAA TTCCAGGTCC TCCTGGCCCC CCAGGACTCC CTGGAAAAGA CGGGTTGTCT 
GGTACACCTG GTGCTGCTGG ATTACGGGGT GAAGATGGAG AGATTGGTCT TCCAGGTGCT 
CCTGGAACAA AGGGTAGCAC TGGTGAGCCA GGACTACCTG GACCTCCTGG ACAATCTGGT 
TTAGCTGGAC TTCCTGGACC AATGGGACCA CGCGGGCTGC CAGGTCCTCC AGGACCACCT 
GGGCCTGTGG GTCATGGATC TGCAATTGGC TTTGATGACA TGGAAGCCTC AGGGCTTGGA 
TTTGGCTTGA GAGAAGGGAC ACAGGGGGTG CCTGGACTTC CCGGGGCAAA GGGAGAGCCA 
GGACATCCAG GGCTGCCAGG AGTGAAGGGA GATAGTGGTT TTCCAGGCCT TGAAGGAAGG 
CCGGGGTTGG ATGGATTCCC AGGTCCAGAG GGATTAAAGG GTGAGAAAGG TGAACGTGGT 
GAGCGGGGAG AATCTGGCCG TGATGGTGTT GGTATTCCAG GTCCACCGGG ACCTCCTGGT 
CAGGTGCTTT ATTTACCATC CGATGACAAG ACTATTGTTG GTGCCATTCC TGGACCAGAG 
GGCAGGCAAG GTGTAGCTGG ACTTCCGGGC CCAAGGGGAC CCAAAGGAGA GGCGGGCTTG 
AACGGTATCC CAGGAACCTT TGGAGTAAAG GGAGAGAAGG GAGAGCCTGG AATTATTATG 
GGACCAGATA GCACCATTTA CACTGATTCA TCTAAAGGAG AGAAGGGTGA CGAAGGACCA 
AGGGGACCAG CAGGAGCTGC GGGACCATTT GGACAAAAGG GAGAAATCGG GTTCCCAGGA 
CGCCCTGGAC GTCCTGGTAT GAATGGACTG AAAGGGGAGA AAGGAGAACC TAGTGGTGCA 
AGTGGTGGCT TTGGACTGAG GGGGCCACCT GGTCCTCCTG GCCCACCCGG TCCTCCTGGC 
AGTGTGGTGT ATGGCGATCC AAATAACCCA GGAGCCCAAG TATATCCAGG TCATAAAGGG 
GAGAGAGGAT TTCCTGGACC CAAAGGTGAT GTTGGTCCTG CTGGCCCACC AGGCTCTTTC 
CCATATGATT TAAGTACCTT TGCCTCGATT TTAAGGAGAG AAGCTGGACT CAAAGGAGAG 
AAGGGCGAAT CTGGGGGTGG TTCCCATGGA CAGGGGATCC CAGGGCCACC TGGAAACCCT 
GGATATCCAG GACTGCCGGG AGCTAAAGGA GACAGTGTTC AAGGTCCTCC AGGCCCAGCC 
GGCCCACCAG GACCTTCTGG AATCGGTTAT GAAGGAAGGC AAGGTCCACC AGGGCCCCCA 
GGTCCTCCAG GCCTGCCAGG TTCCTCCTCT TATCCTGGGC CTCACAGGCA AACCATCAGC 
ATACCTGGAC CTCCTGGCCC ACCAGGCCCA CCAGGGCTCC CAGGAACATC AGGAGGATTA 
TCTGGGGCTA CTGTGCTACA GACCTATCAG ACTATGATCA ACTTAGCCCA CAGGCTGCAG 
GAAGGGACAA TTGTGCTGGT CCAGGAGAGG TCGGAACTGT ATGTGCGTGT GAGAGAAGGC 
TTCCGAAAAA TTCTTCTTGG TGAATATACA ACATCCTCTG GCCCTGGACA GGATAATGAA 
GTTGCTGCGG AGCAGGCCCC TGTAGTTCCA TATCCACACC GTAACAAAGA TTCTGATTAC 
CTCCAGTCTA CAGAGGAGAA CAGCAACAAT CCAAATGTGC CCTCAGGTGC CCGACCCTGG 
AGCGATCCTC GGTTACCAGA ACCACCAAGA GTAAACCCAC CACCACCCCC AGACCATCAC 
CACCATGGAA ACCCTCGTCC ATTGGAACCA TCCCCAGTGC ACACACACCA AGAGTTTAAT 
CCTGTGCTTC ACCTTGTGGC CCTGAACACT CCACTGAGTG GCAGCATGAA ATCCATCCGC 
GGAGTGGACT TCCAGTGCTT TGAGCAAGCC CGTAAAGCTG GACTACATGG CACATTTAGA 
GCGTTTTTGT CTTCCCGACT TCAAGACCTG TACAGCATTG TACGCAGAGC TGATCGACAA 
TCTGTGCAAA TTGTTAATCT TAGGGATGAA GTGTTATTTG ACAACTGGGA GTCCCTGTTC 
TCTGGTTCTG AGGCTCAGAT GAGGCCTGGT GCGAGTATCT TCTCTTTTGA TGGAAAAGAT 
GTTGCAACAG ATCCTACATG GCCACAGAAG ATGGTTTGGC ATGGGTCTGA CTCGAAGGGA 
CGGCGGCTTA CTGAAAGCTA TTGCGAGACC TGGCGCACAG ATGAAGGTGC AGTAACTGGT 
CAGGCATCAT CGCTGACGAG TGGGAAACTC TTAGAACAGC GCCCACAGAG TTGCAACAAG 
AACTTCATTG TTTTGTGTAT TGAGAACAGC TTCATGACCA ATAACCGGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826357.1
CDS	56..4849
Translation	MAPARLSLGVTVLVLLLPALSNGQFWSWFTGSKETTASPTTQRVTPGTSSVHSTAVQHHSPDASPTTLHHIPDALPSAESTSAPAVHQPPEEDNLPETTPSHLEAASVPSSEKPTPFTEQSPTASSSAILIPSTVILTTEGKNDSTETAGVTRPLQTTESPTTSRRQEEKQIWNIFSVQKGKENVAPGTLATTPVAKDETSAPSVSEVPISQKPQSEQNIAGVGAEILNVAEGIRNFVHGWEDNIANSTKQTPTPDTEMVTATTAPSLTVNDTGTLSYNKTEPSENTMANHTEGPVSEPHKLISSHNKPLLPDNFNPEVGLLQLIGEPVPEQITKVFGPDKTPAYVFGKEANTGQVAQYHLPNPMYRDFSLLFHVQPTTDKAGILFAITDASQSIVYIGVKLSEVKDGKQDIIFYYTEPGSQDSYVAATFNVPSLVNRWSRFAISVEEEEVVLFMDCDELERVRFERSPDEMELEDGSGLFVAQAGGADPDRYQGVIADLKIVADPRAAEHQCEEDGDDSDGISGDFGSGIADNDLVTSPPVIIKVQPPITVPPIQRTPVEKEIVQTGQGQQGVKGEKGDRGEKGERGLTGPKGDGGNASPTSDSKGEKGQKGEVGVKGSAGFGYPGPKGQKGDPGDLGPRGPPGPPGPSILQKSDGTTVQQVTGPPGPQGPAGPPGKEGEPGDPGEDGKTGEVGPQGFPGTPGDSGQKGDKGDSGVGIRGAPGPPGPPGPPGPSRTDKLTFIDMEGSGYGGDSEHIRGAPGVPGPPGPPGVPGLPGVPGTFGMNNTGLPGPPGLPGLPGKEGIPGPPGPPGLPGKDGLSGTPGAAGLRGEDGEIGLPGAPGTKGSTGEPGLPGPPGQSGLAGLPGPMGPRGLPGPPGPPGPVGHGSAIGFDDMEASGLGFGLREGTQGVPGLPGAKGEPGHPGLPGVKGDSGFPGLEGRPGLDGFPGPEGLKGEKGERGERGESGRDGVGIPGPPGPPGQVLYLPSDDKTIVGAIPGPEGRQGVAGLPGPRGPKGEAGLNGIPGTFGVKGEKGEPGIIMGPDSTIYTDSSKGEKGDEGPRGPAGAAGPFGQKGEIGFPGRPGRPGMNGLKGEKGEPSGASGGFGLRGPPGPPGPPGPPGSVVYGDPNNPGAQVYPGHKGERGFPGPKGDVGPAGPPGSFPYDLSTFASILRREAGLKGEKGESGGGSHGQGIPGPPGNPGYPGLPGAKGDSVQGPPGPAGPPGPSGIGYEGRQGPPGPPGPPGLPGSSSYPGPHRQTISIPGPPGPPGPPGLPGTSGGLSGATVLQTYQTMINLAHRLQEGTIVLVQERSELYVRVREGFRKILLGEYTTSSGPGQDNEVAAEQAPVVPYPHRNKDSDYLQSTEENSNNPNVPSGARPWSDPRLPEPPRVNPPPPPDHHHHGNPRPLEPSPVHTHQEFNPVLHLVALNTPLSGSMKSIRGVDFQCFEQARKAGLHGTFRAFLSSRLQDLYSIVRRADRQSVQIVNLRDEVLFDNWESLFSGSEAQMRPGASIFSFDGKDVATDPTWPQKMVWHGSDSKGRRLTESYCETWRTDEGAVTGQASSLTSGKLLEQRPQSCNKNFIVLCIENSFMTNNRK

Target ORF information:

RefSeq Version	XM_012970903.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type XVIII, alpha 1 (col18a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970903.2

ORF Insert Sequence:

ATGGCTCCAG CGCGGCTTAG CCTTGGGGTC ACAGTGCTTG TGCTCCTCTT ACCTGCTCTA 
TCCAATGGCC AGTTTTGGTC CTGGTTTACA GGGAGCAAAG AGACAACTGC GAGCCCCACA 
ACTCAACGTG TAACTCCAGG AACATCCTCA GTTCATAGTA CTGCTGTGCA GCATCACTCA 
CCTGATGCCT CGCCAACCAC ATTGCACCAT ATACCGGATG CACTACCCTC AGCGGAAAGT 
ACGTCAGCAC CTGCAGTGCA TCAGCCACCT GAAGAAGATA ACCTGCCTGA GACAACACCC 
TCTCACCTAG AGGCAGCCTC TGTGCCATCT TCAGAAAAAC CCACACCTTT TACAGAACAG 
TCACCCACAG CATCCTCCTC AGCCATACTC ATTCCCAGCA CAGTCATCCT AACCACAGAG 
GGCAAGAATG ACAGTACTGA GACAGCAGGA GTGACCAGAC CCCTCCAAAC GACAGAGTCC 
CCTACTACTT CCAGGAGGCA GGAGGAAAAG CAAATATGGA ACATATTCAG TGTGCAGAAA 
GGAAAGGAGA ACGTAGCGCC TGGGACTTTG GCCACAACAC CTGTAGCGAA GGATGAAACC 
AGTGCACCTT CTGTGTCAGA GGTTCCAATA TCTCAGAAGC CACAAAGTGA ACAAAACATT 
GCTGGTGTAG GGGCCGAGAT CTTAAATGTA GCAGAAGGAA TTAGGAACTT TGTGCATGGC 
TGGGAAGATA ACATTGCCAA CAGCACAAAG CAGACTCCTA CTCCTGACAC GGAGATGGTG 
ACTGCCACAA CAGCCCCAAG CCTTACTGTA AATGACACTG GCACCTTAAG CTACAACAAG 
ACAGAACCAA GCGAGAACAC TATGGCAAAC CATACGGAAG GTCCGGTGTC CGAGCCCCAT 
AAACTGATCT CATCCCACAA CAAGCCACTT CTACCAGACA ACTTCAACCC AGAAGTTGGC 
CTCTTGCAGT TGATTGGAGA ACCTGTACCT GAACAGATCA CCAAGGTCTT TGGGCCCGAC 
AAGACTCCCG CCTATGTATT TGGTAAAGAA GCCAATACAG GCCAGGTGGC CCAATACCAT 
CTGCCCAACC CTATGTATAG GGACTTCTCT CTCCTCTTCC ATGTCCAGCC AACCACAGAC 
AAGGCCGGCA TCCTCTTTGC CATCACAGAT GCCTCTCAGT CTATTGTGTA TATCGGGGTG 
AAGCTTTCTG AAGTCAAGGA TGGGAAGCAA GACATCATCT TCTACTATAC AGAGCCTGGC 
TCACAGGATT CCTATGTAGC TGCCACCTTC AATGTCCCAT CACTGGTGAA TCGTTGGTCT 
CGCTTTGCAA TCAGTGTGGA GGAAGAAGAA GTTGTGCTGT TTATGGATTG CGATGAGCTG 
GAAAGGGTGC GCTTCGAGAG ATCACCTGAT GAGATGGAAC TAGAAGATGG CTCTGGGCTC 
TTTGTTGCTC AAGCAGGTGG CGCTGATCCA GACAGATATC AGGGTGTAAT AGCAGATCTA 
AAGATCGTAG CCGATCCAAG AGCCGCAGAA CATCAGTGTG AGGAGGATGG AGATGACTCT 
GATGGGATCT CTGGAGATTT TGGCAGTGGC ATTGCGGACA ATGATTTGGT GACTTCTCCT 
CCTGTGATAA TCAAAGTGCA GCCTCCTATA ACTGTCCCTC CCATACAGAG GACCCCTGTG 
GAGAAAGAGA TTGTTCAGAC AGGTCAAGGC CAGCAAGGGG TAAAAGGAGA GAAGGGTGAT 
CGTGGAGAGA AGGGTGAGAG GGGACTGACT GGCCCTAAGG GAGACGGTGG GAATGCAAGT 
CCAACTTCTG ATTCCAAAGG AGAAAAGGGT CAGAAAGGAG AGGTCGGAGT GAAGGGAAGT 
GCAGGCTTTG GCTATCCCGG GCCTAAAGGG CAGAAAGGAG ATCCTGGAGA CTTGGGACCA 
AGAGGACCAC CAGGCCCACC TGGACCATCT ATATTACAGA AAAGTGATGG AACTACAGTG 
CAACAGGTCA CAGGACCTCC AGGTCCTCAA GGCCCTGCGG GACCACCAGG AAAAGAAGGG 
GAACCAGGTG ACCCTGGTGA AGATGGAAAA ACTGGCGAGG TTGGTCCCCA AGGATTTCCA 
GGGACCCCAG GAGATTCTGG GCAGAAGGGA GACAAGGGTG ATTCTGGTGT TGGTATCAGA 
GGTGCTCCTG GGCCACCAGG TCCGCCAGGC CCCCCTGGAC CTTCTAGAAC CGACAAGCTG 
ACATTCATTG ACATGGAAGG TTCTGGATAT GGTGGAGATT CGGAGCACAT CAGGGGAGCC 
CCTGGTGTGC CTGGGCCCCC AGGTCCTCCA GGTGTACCAG GGTTACCCGG AGTACCCGGA 
ACTTTTGGCA TGAACAACAC AGGATTGCCA GGTCCACCTG GATTGCCAGG TCTTCCCGGA 
AAGGAAGGAA TTCCAGGTCC TCCTGGCCCC CCAGGACTCC CTGGAAAAGA CGGGTTGTCT 
GGTACACCTG GTGCTGCTGG ATTACGGGGT GAAGATGGAG AGATTGGTCT TCCAGGTGCT 
CCTGGAACAA AGGGTAGCAC TGGTGAGCCA GGACTACCTG GACCTCCTGG ACAATCTGGT 
TTAGCTGGAC TTCCTGGACC AATGGGACCA CGCGGGCTGC CAGGTCCTCC AGGACCACCT 
GGGCCTGTGG GTCATGGATC TGCAATTGGC TTTGATGACA TGGAAGCCTC AGGGCTTGGA 
TTTGGCTTGA GAGAAGGGAC ACAGGGGGTG CCTGGACTTC CCGGGGCAAA GGGAGAGCCA 
GGACATCCAG GGCTGCCAGG AGTGAAGGGA GATAGTGGTT TTCCAGGCCT TGAAGGAAGG 
CCGGGGTTGG ATGGATTCCC AGGTCCAGAG GGATTAAAGG GTGAGAAAGG TGAACGTGGT 
GAGCGGGGAG AATCTGGCCG TGATGGTGTT GGTATTCCAG GTCCACCGGG ACCTCCTGGT 
CAGGTGCTTT ATTTACCATC CGATGACAAG ACTATTGTTG GTGCCATTCC TGGACCAGAG 
GGCAGGCAAG GTGTAGCTGG ACTTCCGGGC CCAAGGGGAC CCAAAGGAGA GGCGGGCTTG 
AACGGTATCC CAGGAACCTT TGGAGTAAAG GGAGAGAAGG GAGAGCCTGG AATTATTATG 
GGACCAGATA GCACCATTTA CACTGATTCA TCTAAAGGAG AGAAGGGTGA CGAAGGACCA 
AGGGGACCAG CAGGAGCTGC GGGACCATTT GGACAAAAGG GAGAAATCGG GTTCCCAGGA 
CGCCCTGGAC GTCCTGGTAT GAATGGACTG AAAGGGGAGA AAGGAGAACC TAGTGGTGCA 
AGTGGTGGCT TTGGACTGAG GGGGCCACCT GGTCCTCCTG GCCCACCCGG TCCTCCTGGC 
AGTGTGGTGT ATGGCGATCC AAATAACCCA GGAGCCCAAG TATATCCAGG TCATAAAGGG 
GAGAGAGGAT TTCCTGGACC CAAAGGTGAT GTTGGTCCTG CTGGCCCACC AGGCTCTTTC 
CCATATGATT TAAGTACCTT TGCCTCGATT TTAAGGAGAG AAGCTGGACT CAAAGGAGAG 
AAGGGCGAAT CTGGGGGTGG TTCCCATGGA CAGGGGATCC CAGGGCCACC TGGAAACCCT 
GGATATCCAG GACTGCCGGG AGCTAAAGGA GACAGTGTTC AAGGTCCTCC AGGCCCAGCC 
GGCCCACCAG GACCTTCTGG AATCGGTTAT GAAGGAAGGC AAGGTCCACC AGGGCCCCCA 
GGTCCTCCAG GCCTGCCAGG TTCCTCCTCT TATCCTGGGC CTCACAGGCA AACCATCAGC 
ATACCTGGAC CTCCTGGCCC ACCAGGCCCA CCAGGGCTCC CAGGAACATC AGGAGGATTA 
TCTGGGGCTA CTGTGCTACA GACCTATCAG ACTATGATCA ACTTAGCCCA CAGGCTGCAG 
GAAGGGACAA TTGTGCTGGT CCAGGAGAGG TCGGAACTGT ATGTGCGTGT GAGAGAAGGC 
TTCCGAAAAA TTCTTCTTGG TGAATATACA ACATCCTCTG GCCCTGGACA GGATAATGAA 
GTTGCTGCGG AGCAGGCCCC TGTAGTTCCA TATCCACACC GTAACAAAGA TTCTGATTAC 
CTCCAGTCTA CAGAGGAGAA CAGCAACAAT CCAAATGTGC CCTCAGGTGC CCGACCCTGG 
AGCGATCCTC GGTTACCAGA ACCACCAAGA GTAAACCCAC CACCACCCCC AGACCATCAC 
CACCATGGAA ACCCTCGTCC ATTGGAACCA TCCCCAGTGC ACACACACCA AGAGTTTAAT 
CCTGTGCTTC ACCTTGTGGC CCTGAACACT CCACTGAGTG GCAGCATGAA ATCCATCCGC 
GGAGTGGACT TCCAGTGCTT TGAGCAAGCC CGTAAAGCTG GACTACATGG CACATTTAGA 
GCGTTTTTGT CTTCCCGACT TCAAGACCTG TACAGCATTG TACGCAGAGC TGATCGACAA 
TCTGTGCAAA TTGTTAATCT TAGGGATGAA GTGTTATTTG ACAACTGGGA GTCCCTGTTC 
TCTGGTTCTG AGGCTCAGAT GAGGCCTGGT GCGAGTATCT TCTCTTTTGA TGGAAAAGAT 
GTTGCAACAG ATCCTACATG GCCACAGAAG ATGGTTTGGC ATGGGTCTGA CTCGAAGGGA 
CGGCGGCTTA CTGAAAGCTA TTGCGAGACC TGGCGCACAG ATGAAGGTGC AGTAACTGGT 
CAGGCATCAT CGCTGACGAG TGGGAAACTC TTAGAACAGC GCCCACAGAG TTGCAACAAG 
AACTTCATTG TTTTGTGTAT TGAGAACAGC TTCATGACCA ATAACCGGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col18a1 ( XM_012970904.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826358.1 Xenopus tropicalis collagen, type XVIII, alpha 1 (col18a1), transcript variant X2, mRNA.

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CloneID	OXa26098
Clone ID Related Accession (Same CDS sequence)	XM_012970904.2
Accession Version	XM_012970904.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(XVIII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970904.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCAG CGCGGCTTAG CCTTGGGGTC ACAGTGCTTG TGCTCCTCTT ACCTGCTCTA 
TCCAATGGCC AGTTTTGGTC CTGGTTTACA GGGAGCAAAG AGACAACTGC GAGCCCCACA 
ACTCAACGTG TAACTCCAGG AACATCCTCA GTTCATAGTA CTGCTGTGCA GCATCACTCA 
CCTGATGCCT CGCCAACCAC ATTGCACCAT ATACCGGATG CACTACCCTC AGCGGAAAGT 
ACGTCAGCAC CTGCAGTGCA TCAGCCACCT GAAGAAGATA ACCTGCCTGA GACAACACCC 
TCTCACCTAG AGGCAGCCTC TGTGCCATCT TCAGAAAAAC CCACACCTTT TACAGAACAG 
TCACCCACAG CATCCTCCTC AGCCATACTC ATTCCCAGCA CAGTCATCCT AACCACAGAG 
GGCAAGAATG ACAGTACTGA GACAGCAGGA GTGACCAGAC CCCTCCAAAC GACAGAGTCC 
CCTACTACTT CCAGGAGGCA GGAGGAAAAG CAAATATGGA ACATATTCAG TGTGCAGAAA 
GGAAAGGAGA ACGTAGCGCC TGGGACTTTG GCCACAACAC CTGTAGCGAA GGATGAAACC 
AGTGCACCTT CTGTGTCAGA GGTTCCAATA TCTCAGAAGC CACAAAGTGA ACAAAACATT 
GCTGGTGTAG GGGCCGAGAT CTTAAATGTA GCAGAAGGAA TTAGGAACTT TGTGCATGGC 
TGGGAAGATA ACATTGCCAA CAGCACAAAG CAGACTCCTA CTCCTGACAC GGAGATGGTG 
ACTGCCACAA CAGCCCCAAG CCTTACTGTA AATGACACTG GCACCTTAAG CTACAACAAG 
ACAGAACCAA GCGAGAACAC TATGGCAAAC CATACGGAAG GTCCGGTGTC CGAGCCCCAT 
AAACTGATCT CATCCCACAA CAAGCCACTT CTACCAGACA ACTTCAACCC AGAAGTTGGC 
CTCTTGCAGT TGATTGGAGA ACCTGTACCT GAACAGATCA CCAAGGTCTT TGGGCCCGAC 
AAGACTCCCG CCTATGTATT TGGTAAAGAA GCCAATACAG GCCAGGTGGC CCAATACCAT 
CTGCCCAACC CTATGTATAG GGACTTCTCT CTCCTCTTCC ATGTCCAGCC AACCACAGAC 
AAGGCCGGCA TCCTCTTTGC CATCACAGAT GCCTCTCAGT CTATTGTGTA TATCGGGGTG 
AAGCTTTCTG AAGTCAAGGA TGGGAAGCAA GACATCATCT TCTACTATAC AGAGCCTGGC 
TCACAGGATT CCTATGTAGC TGCCACCTTC AATGTCCCAT CACTGGTGAA TCGTTGGTCT 
CGCTTTGCAA TCAGTGTGGA GGAAGAAGAA GTTGTGCTGT TTATGGATTG CGATGAGCTG 
GAAAGGGTGC GCTTCGAGAG ATCACCTGAT GAGATGGAAC TAGAAGATGG CTCTGGGCTC 
TTTGTTGCTC AAGCAGGTGG CGCTGATCCA GACAGATATC AGGGTGTAAT AGCAGATCTA 
AAGATCGTAG CCGATCCAAG AGCCGCAGAA CATCAGTGTG AGGAGGATGG AGATGACTCT 
GATGGGATCT CTGGAGATTT TGGCAGTGGC ATTGCGGACA ATGATTTGGT GACTTCTCCT 
CCTGTGATAA TCAAAGTGCA GCCTCCTATA ACTGTCCCTC CCATACAGAG GACCCCTGTG 
GAGAAAGAGA TTGTTCAGAC AGGTCAAGGC CAGCAAGGGG TAAAAGGAGA GAAGGGTGAT 
CGTGGAGAGA AGGGTGAGAG GGGACTGACT GGCCCTAAGG GAGACGGTGG GAATGCAAGT 
CCAACTTCTG ATTCCAAAGG AGAAAAGGGT CAGAAAGGAG AGGTCGGAGT GAAGGGAAGT 
GCAGGCTTTG GCTATCCCGG GCCTAAAGGG CAGAAAGGAG ATCCTGGAGA CTTGGGACCA 
AGAGGACCAC CAGGCCCACC TGGACCATCT ATATTACAGA AAAGTGATGG AACTACAGTG 
CAACAGGTCA CAGGACCTCC AGGTCCTCAA GGCCCTGCGG GACCACCAGG AAAAGAAGGG 
GAACCAGGTG ACCCTGGTGA AGATGGAAAA ACTGGCGAGG TTGGTCCCCA AGGATTTCCA 
GGGACCCCAG GAGATTCTGG GCAGAAGGGA GACAAGGGTG ATTCTGGTGT TGGTATCAGA 
GGTGCTCCTG GGCCACCAGG TCCGCCAGGC CCCCCTGGAC CTTCTAGAAC CGACAAGCTG 
ACATTCATTG ACATGGAAGG TTCTGGATAT GGTGGAGATT CGGAGCACAT CAGGGGAGCC 
CCTGGTGTGC CTGGGCCCCC AGGTCCTCCA GGTGTACCAG GGTTACCCGG AGTACCCGGA 
ACTTTTGGCA TGAACAACAC AGGATTGCCA GGTCCACCTG GATTGCCAGG TCTTCCCGGA 
AAGGAAGGAA TTCCAGGTCC TCCTGGCCCC CCAGGACTCC CTGGAAAAGA CGGGTTGTCT 
GGTACACCTG GTGCTGCTGG ATTACGGGGT GAAGATGGAG AGATTGGTCT TCCAGGTGCT 
CCTGGAACAA AGGGTAGCAC TGGTGAGCCA GGACTACCTG GACCTCCTGG ACAATCTGGT 
TTAGCTGGAC TTCCTGGACC AATGGGACCA CGCGGGCTGC CAGGTCCTCC AGGACCACCT 
GGGCCTGTGG GTCATGGATC TGCAATTGGC TTTGATGACA TGGAAGCCTC AGGGCTTGGA 
TTTGGCTTGA GAGAAGGGAC ACAGGGGGTG CCTGGACTTC CCGGGGCAAA GGGAGAGCCA 
GGACATCCAG GGCTGCCAGG AGTGAAGGGA GATAGTGGTT TTCCAGGCCT TGAAGGAAGG 
CCGGGGTTGG ATGGATTCCC AGGTCCAGAG GGATTAAAGG GTGAGAAAGG TGAACGTGGT 
GAGCGGGGAG AATCTGGCCG TGATGGTGTT GGTATTCCAG GTCCACCGGG ACCTCCTGGT 
CAGGTGCTTT ATTTACCATC CGATGACAAG ACTATTGTTG GTGCCATTCC TGGACCAGAG 
GGCCCAAGGG GACCCAAAGG AGAGGCGGGC TTGAACGGTA TCCCAGGAAC CTTTGGAGTA 
AAGGGAGAGA AGGGAGAGCC TGGAATTATT ATGGGACCAG ATAGCACCAT TTACACTGAT 
TCATCTAAAG GAGAGAAGGG TGACGAAGGA CCAAGGGGAC CAGCAGGAGC TGCGGGACCA 
TTTGGACAAA AGGGAGAAAT CGGGTTCCCA GGACGCCCTG GACGTCCTGG TATGAATGGA 
CTGAAAGGGG AGAAAGGAGA ACCTAGTGGT GCAAGTGGTG GCTTTGGACT GAGGGGGCCA 
CCTGGTCCTC CTGGCCCACC CGGTCCTCCT GGCAGTGTGG TGTATGGCGA TCCAAATAAC 
CCAGGAGCCC AAGTATATCC AGGTCATAAA GGGGAGAGAG GATTTCCTGG ACCCAAAGGT 
GATGTTGGTC CTGCTGGCCC ACCAGGCTCT TTCCCATATG ATTTAAGTAC CTTTGCCTCG 
ATTTTAAGGA GAGAAGCTGG ACTCAAAGGA GAGAAGGGCG AATCTGGGGG TGGTTCCCAT 
GGACAGGGGA TCCCAGGGCC ACCTGGAAAC CCTGGATATC CAGGACTGCC GGGAGCTAAA 
GGAGACAGTG TTCAAGGTCC TCCAGGCCCA GCCGGCCCAC CAGGACCTTC TGGAATCGGT 
TATGAAGGAA GGCAAGGTCC ACCAGGGCCC CCAGGTCCTC CAGGCCTGCC AGGTTCCTCC 
TCTTATCCTG GGCCTCACAG GCAAACCATC AGCATACCTG GACCTCCTGG CCCACCAGGC 
CCACCAGGGC TCCCAGGAAC ATCAGGAGGA TTATCTGGGG CTACTGTGCT ACAGACCTAT 
CAGACTATGA TCAACTTAGC CCACAGGCTG CAGGAAGGGA CAATTGTGCT GGTCCAGGAG 
AGGTCGGAAC TGTATGTGCG TGTGAGAGAA GGCTTCCGAA AAATTCTTCT TGGTGAATAT 
ACAACATCCT CTGGCCCTGG ACAGGATAAT GAAGTTGCTG CGGAGCAGGC CCCTGTAGTT 
CCATATCCAC ACCGTAACAA AGATTCTGAT TACCTCCAGT CTACAGAGGA GAACAGCAAC 
AATCCAAATG TGCCCTCAGG TGCCCGACCC TGGAGCGATC CTCGGTTACC AGAACCACCA 
AGAGTAAACC CACCACCACC CCCAGACCAT CACCACCATG GAAACCCTCG TCCATTGGAA 
CCATCCCCAG TGCACACACA CCAAGAGTTT AATCCTGTGC TTCACCTTGT GGCCCTGAAC 
ACTCCACTGA GTGGCAGCAT GAAATCCATC CGCGGAGTGG ACTTCCAGTG CTTTGAGCAA 
GCCCGTAAAG CTGGACTACA TGGCACATTT AGAGCGTTTT TGTCTTCCCG ACTTCAAGAC 
CTGTACAGCA TTGTACGCAG AGCTGATCGA CAATCTGTGC AAATTGTTAA TCTTAGGGAT 
GAAGTGTTAT TTGACAACTG GGAGTCCCTG TTCTCTGGTT CTGAGGCTCA GATGAGGCCT 
GGTGCGAGTA TCTTCTCTTT TGATGGAAAA GATGTTGCAA CAGATCCTAC ATGGCCACAG 
AAGATGGTTT GGCATGGGTC TGACTCGAAG GGACGGCGGC TTACTGAAAG CTATTGCGAG 
ACCTGGCGCA CAGATGAAGG TGCAGTAACT GGTCAGGCAT CATCGCTGAC GAGTGGGAAA 
CTCTTAGAAC AGCGCCCACA GAGTTGCAAC AAGAACTTCA TTGTTTTGTG TATTGAGAAC 
AGCTTCATGA CCAATAACCG GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826358.1
CDS	56..4822
Translation	MAPARLSLGVTVLVLLLPALSNGQFWSWFTGSKETTASPTTQRVTPGTSSVHSTAVQHHSPDASPTTLHHIPDALPSAESTSAPAVHQPPEEDNLPETTPSHLEAASVPSSEKPTPFTEQSPTASSSAILIPSTVILTTEGKNDSTETAGVTRPLQTTESPTTSRRQEEKQIWNIFSVQKGKENVAPGTLATTPVAKDETSAPSVSEVPISQKPQSEQNIAGVGAEILNVAEGIRNFVHGWEDNIANSTKQTPTPDTEMVTATTAPSLTVNDTGTLSYNKTEPSENTMANHTEGPVSEPHKLISSHNKPLLPDNFNPEVGLLQLIGEPVPEQITKVFGPDKTPAYVFGKEANTGQVAQYHLPNPMYRDFSLLFHVQPTTDKAGILFAITDASQSIVYIGVKLSEVKDGKQDIIFYYTEPGSQDSYVAATFNVPSLVNRWSRFAISVEEEEVVLFMDCDELERVRFERSPDEMELEDGSGLFVAQAGGADPDRYQGVIADLKIVADPRAAEHQCEEDGDDSDGISGDFGSGIADNDLVTSPPVIIKVQPPITVPPIQRTPVEKEIVQTGQGQQGVKGEKGDRGEKGERGLTGPKGDGGNASPTSDSKGEKGQKGEVGVKGSAGFGYPGPKGQKGDPGDLGPRGPPGPPGPSILQKSDGTTVQQVTGPPGPQGPAGPPGKEGEPGDPGEDGKTGEVGPQGFPGTPGDSGQKGDKGDSGVGIRGAPGPPGPPGPPGPSRTDKLTFIDMEGSGYGGDSEHIRGAPGVPGPPGPPGVPGLPGVPGTFGMNNTGLPGPPGLPGLPGKEGIPGPPGPPGLPGKDGLSGTPGAAGLRGEDGEIGLPGAPGTKGSTGEPGLPGPPGQSGLAGLPGPMGPRGLPGPPGPPGPVGHGSAIGFDDMEASGLGFGLREGTQGVPGLPGAKGEPGHPGLPGVKGDSGFPGLEGRPGLDGFPGPEGLKGEKGERGERGESGRDGVGIPGPPGPPGQVLYLPSDDKTIVGAIPGPEGPRGPKGEAGLNGIPGTFGVKGEKGEPGIIMGPDSTIYTDSSKGEKGDEGPRGPAGAAGPFGQKGEIGFPGRPGRPGMNGLKGEKGEPSGASGGFGLRGPPGPPGPPGPPGSVVYGDPNNPGAQVYPGHKGERGFPGPKGDVGPAGPPGSFPYDLSTFASILRREAGLKGEKGESGGGSHGQGIPGPPGNPGYPGLPGAKGDSVQGPPGPAGPPGPSGIGYEGRQGPPGPPGPPGLPGSSSYPGPHRQTISIPGPPGPPGPPGLPGTSGGLSGATVLQTYQTMINLAHRLQEGTIVLVQERSELYVRVREGFRKILLGEYTTSSGPGQDNEVAAEQAPVVPYPHRNKDSDYLQSTEENSNNPNVPSGARPWSDPRLPEPPRVNPPPPPDHHHHGNPRPLEPSPVHTHQEFNPVLHLVALNTPLSGSMKSIRGVDFQCFEQARKAGLHGTFRAFLSSRLQDLYSIVRRADRQSVQIVNLRDEVLFDNWESLFSGSEAQMRPGASIFSFDGKDVATDPTWPQKMVWHGSDSKGRRLTESYCETWRTDEGAVTGQASSLTSGKLLEQRPQSCNKNFIVLCIENSFMTNNRK

Target ORF information:

RefSeq Version	XM_012970904.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type XVIII, alpha 1 (col18a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970904.2

ORF Insert Sequence:

ATGGCTCCAG CGCGGCTTAG CCTTGGGGTC ACAGTGCTTG TGCTCCTCTT ACCTGCTCTA 
TCCAATGGCC AGTTTTGGTC CTGGTTTACA GGGAGCAAAG AGACAACTGC GAGCCCCACA 
ACTCAACGTG TAACTCCAGG AACATCCTCA GTTCATAGTA CTGCTGTGCA GCATCACTCA 
CCTGATGCCT CGCCAACCAC ATTGCACCAT ATACCGGATG CACTACCCTC AGCGGAAAGT 
ACGTCAGCAC CTGCAGTGCA TCAGCCACCT GAAGAAGATA ACCTGCCTGA GACAACACCC 
TCTCACCTAG AGGCAGCCTC TGTGCCATCT TCAGAAAAAC CCACACCTTT TACAGAACAG 
TCACCCACAG CATCCTCCTC AGCCATACTC ATTCCCAGCA CAGTCATCCT AACCACAGAG 
GGCAAGAATG ACAGTACTGA GACAGCAGGA GTGACCAGAC CCCTCCAAAC GACAGAGTCC 
CCTACTACTT CCAGGAGGCA GGAGGAAAAG CAAATATGGA ACATATTCAG TGTGCAGAAA 
GGAAAGGAGA ACGTAGCGCC TGGGACTTTG GCCACAACAC CTGTAGCGAA GGATGAAACC 
AGTGCACCTT CTGTGTCAGA GGTTCCAATA TCTCAGAAGC CACAAAGTGA ACAAAACATT 
GCTGGTGTAG GGGCCGAGAT CTTAAATGTA GCAGAAGGAA TTAGGAACTT TGTGCATGGC 
TGGGAAGATA ACATTGCCAA CAGCACAAAG CAGACTCCTA CTCCTGACAC GGAGATGGTG 
ACTGCCACAA CAGCCCCAAG CCTTACTGTA AATGACACTG GCACCTTAAG CTACAACAAG 
ACAGAACCAA GCGAGAACAC TATGGCAAAC CATACGGAAG GTCCGGTGTC CGAGCCCCAT 
AAACTGATCT CATCCCACAA CAAGCCACTT CTACCAGACA ACTTCAACCC AGAAGTTGGC 
CTCTTGCAGT TGATTGGAGA ACCTGTACCT GAACAGATCA CCAAGGTCTT TGGGCCCGAC 
AAGACTCCCG CCTATGTATT TGGTAAAGAA GCCAATACAG GCCAGGTGGC CCAATACCAT 
CTGCCCAACC CTATGTATAG GGACTTCTCT CTCCTCTTCC ATGTCCAGCC AACCACAGAC 
AAGGCCGGCA TCCTCTTTGC CATCACAGAT GCCTCTCAGT CTATTGTGTA TATCGGGGTG 
AAGCTTTCTG AAGTCAAGGA TGGGAAGCAA GACATCATCT TCTACTATAC AGAGCCTGGC 
TCACAGGATT CCTATGTAGC TGCCACCTTC AATGTCCCAT CACTGGTGAA TCGTTGGTCT 
CGCTTTGCAA TCAGTGTGGA GGAAGAAGAA GTTGTGCTGT TTATGGATTG CGATGAGCTG 
GAAAGGGTGC GCTTCGAGAG ATCACCTGAT GAGATGGAAC TAGAAGATGG CTCTGGGCTC 
TTTGTTGCTC AAGCAGGTGG CGCTGATCCA GACAGATATC AGGGTGTAAT AGCAGATCTA 
AAGATCGTAG CCGATCCAAG AGCCGCAGAA CATCAGTGTG AGGAGGATGG AGATGACTCT 
GATGGGATCT CTGGAGATTT TGGCAGTGGC ATTGCGGACA ATGATTTGGT GACTTCTCCT 
CCTGTGATAA TCAAAGTGCA GCCTCCTATA ACTGTCCCTC CCATACAGAG GACCCCTGTG 
GAGAAAGAGA TTGTTCAGAC AGGTCAAGGC CAGCAAGGGG TAAAAGGAGA GAAGGGTGAT 
CGTGGAGAGA AGGGTGAGAG GGGACTGACT GGCCCTAAGG GAGACGGTGG GAATGCAAGT 
CCAACTTCTG ATTCCAAAGG AGAAAAGGGT CAGAAAGGAG AGGTCGGAGT GAAGGGAAGT 
GCAGGCTTTG GCTATCCCGG GCCTAAAGGG CAGAAAGGAG ATCCTGGAGA CTTGGGACCA 
AGAGGACCAC CAGGCCCACC TGGACCATCT ATATTACAGA AAAGTGATGG AACTACAGTG 
CAACAGGTCA CAGGACCTCC AGGTCCTCAA GGCCCTGCGG GACCACCAGG AAAAGAAGGG 
GAACCAGGTG ACCCTGGTGA AGATGGAAAA ACTGGCGAGG TTGGTCCCCA AGGATTTCCA 
GGGACCCCAG GAGATTCTGG GCAGAAGGGA GACAAGGGTG ATTCTGGTGT TGGTATCAGA 
GGTGCTCCTG GGCCACCAGG TCCGCCAGGC CCCCCTGGAC CTTCTAGAAC CGACAAGCTG 
ACATTCATTG ACATGGAAGG TTCTGGATAT GGTGGAGATT CGGAGCACAT CAGGGGAGCC 
CCTGGTGTGC CTGGGCCCCC AGGTCCTCCA GGTGTACCAG GGTTACCCGG AGTACCCGGA 
ACTTTTGGCA TGAACAACAC AGGATTGCCA GGTCCACCTG GATTGCCAGG TCTTCCCGGA 
AAGGAAGGAA TTCCAGGTCC TCCTGGCCCC CCAGGACTCC CTGGAAAAGA CGGGTTGTCT 
GGTACACCTG GTGCTGCTGG ATTACGGGGT GAAGATGGAG AGATTGGTCT TCCAGGTGCT 
CCTGGAACAA AGGGTAGCAC TGGTGAGCCA GGACTACCTG GACCTCCTGG ACAATCTGGT 
TTAGCTGGAC TTCCTGGACC AATGGGACCA CGCGGGCTGC CAGGTCCTCC AGGACCACCT 
GGGCCTGTGG GTCATGGATC TGCAATTGGC TTTGATGACA TGGAAGCCTC AGGGCTTGGA 
TTTGGCTTGA GAGAAGGGAC ACAGGGGGTG CCTGGACTTC CCGGGGCAAA GGGAGAGCCA 
GGACATCCAG GGCTGCCAGG AGTGAAGGGA GATAGTGGTT TTCCAGGCCT TGAAGGAAGG 
CCGGGGTTGG ATGGATTCCC AGGTCCAGAG GGATTAAAGG GTGAGAAAGG TGAACGTGGT 
GAGCGGGGAG AATCTGGCCG TGATGGTGTT GGTATTCCAG GTCCACCGGG ACCTCCTGGT 
CAGGTGCTTT ATTTACCATC CGATGACAAG ACTATTGTTG GTGCCATTCC TGGACCAGAG 
GGCCCAAGGG GACCCAAAGG AGAGGCGGGC TTGAACGGTA TCCCAGGAAC CTTTGGAGTA 
AAGGGAGAGA AGGGAGAGCC TGGAATTATT ATGGGACCAG ATAGCACCAT TTACACTGAT 
TCATCTAAAG GAGAGAAGGG TGACGAAGGA CCAAGGGGAC CAGCAGGAGC TGCGGGACCA 
TTTGGACAAA AGGGAGAAAT CGGGTTCCCA GGACGCCCTG GACGTCCTGG TATGAATGGA 
CTGAAAGGGG AGAAAGGAGA ACCTAGTGGT GCAAGTGGTG GCTTTGGACT GAGGGGGCCA 
CCTGGTCCTC CTGGCCCACC CGGTCCTCCT GGCAGTGTGG TGTATGGCGA TCCAAATAAC 
CCAGGAGCCC AAGTATATCC AGGTCATAAA GGGGAGAGAG GATTTCCTGG ACCCAAAGGT 
GATGTTGGTC CTGCTGGCCC ACCAGGCTCT TTCCCATATG ATTTAAGTAC CTTTGCCTCG 
ATTTTAAGGA GAGAAGCTGG ACTCAAAGGA GAGAAGGGCG AATCTGGGGG TGGTTCCCAT 
GGACAGGGGA TCCCAGGGCC ACCTGGAAAC CCTGGATATC CAGGACTGCC GGGAGCTAAA 
GGAGACAGTG TTCAAGGTCC TCCAGGCCCA GCCGGCCCAC CAGGACCTTC TGGAATCGGT 
TATGAAGGAA GGCAAGGTCC ACCAGGGCCC CCAGGTCCTC CAGGCCTGCC AGGTTCCTCC 
TCTTATCCTG GGCCTCACAG GCAAACCATC AGCATACCTG GACCTCCTGG CCCACCAGGC 
CCACCAGGGC TCCCAGGAAC ATCAGGAGGA TTATCTGGGG CTACTGTGCT ACAGACCTAT 
CAGACTATGA TCAACTTAGC CCACAGGCTG CAGGAAGGGA CAATTGTGCT GGTCCAGGAG 
AGGTCGGAAC TGTATGTGCG TGTGAGAGAA GGCTTCCGAA AAATTCTTCT TGGTGAATAT 
ACAACATCCT CTGGCCCTGG ACAGGATAAT GAAGTTGCTG CGGAGCAGGC CCCTGTAGTT 
CCATATCCAC ACCGTAACAA AGATTCTGAT TACCTCCAGT CTACAGAGGA GAACAGCAAC 
AATCCAAATG TGCCCTCAGG TGCCCGACCC TGGAGCGATC CTCGGTTACC AGAACCACCA 
AGAGTAAACC CACCACCACC CCCAGACCAT CACCACCATG GAAACCCTCG TCCATTGGAA 
CCATCCCCAG TGCACACACA CCAAGAGTTT AATCCTGTGC TTCACCTTGT GGCCCTGAAC 
ACTCCACTGA GTGGCAGCAT GAAATCCATC CGCGGAGTGG ACTTCCAGTG CTTTGAGCAA 
GCCCGTAAAG CTGGACTACA TGGCACATTT AGAGCGTTTT TGTCTTCCCG ACTTCAAGAC 
CTGTACAGCA TTGTACGCAG AGCTGATCGA CAATCTGTGC AAATTGTTAA TCTTAGGGAT 
GAAGTGTTAT TTGACAACTG GGAGTCCCTG TTCTCTGGTT CTGAGGCTCA GATGAGGCCT 
GGTGCGAGTA TCTTCTCTTT TGATGGAAAA GATGTTGCAA CAGATCCTAC ATGGCCACAG 
AAGATGGTTT GGCATGGGTC TGACTCGAAG GGACGGCGGC TTACTGAAAG CTATTGCGAG 
ACCTGGCGCA CAGATGAAGG TGCAGTAACT GGTCAGGCAT CATCGCTGAC GAGTGGGAAA 
CTCTTAGAAC AGCGCCCACA GAGTTGCAAC AAGAACTTCA TTGTTTTGTG TATTGAGAAC 
AGCTTCATGA CCAATAACCG GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col18a1 ( XM_012970905.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826359.1 Xenopus tropicalis collagen, type XVIII, alpha 1 (col18a1), transcript variant X3, mRNA.

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CloneID	OXa26099
Clone ID Related Accession (Same CDS sequence)	XM_012970905.1
Accession Version	XM_012970905.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(XVIII) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCATTT CCATTCCACA AGTAACCTCT CTTTCCAGTG GCCTATCCCA TGATGTGTTA 
AATTCATCCC AGTTGAATGG GAATAACATA GGCAAACAGA GCACTTACAC CACCAATCAG 
GTTTATAAGA GCCCTGAGCT CCTGAAACAT TCTGGGATAG CTGCCACTCT TCATGGTGTT 
TCTGCACCTA ACAATTATGT CACTAGTCTA ACGGGACATC CCCATCCATC TGAAACTGAT 
TTACTAACCA GCACTAAATC TCCCATTCCA TCTTTTAGCC ATAATAGCTC CAGGACTGCT 
TTTATCTTGC CCAGATTGAC TCCATCAAAT TCTCGCTGCT TGCCTACCCC TACCAGCCTC 
TCATTCTGCA AAAGATTAGG AATTGACTCC TTTCTGCTCC CCAATTATCT GAACCATAGT 
ACTGTGCTGG AAATTCAGGC AGCTTTGCAT GATTGGGAGG GGCTGTTTAA ATCTCAGTGC 
CACCGCTACC TGGAATCGTT TTTCTGCTTG CTTCTGGCAC CAAGTTGTAA TGCTGGCACT 
GTGGCACCTC CGCTGGTGCC ATGCCGTCAG TTTTGTGAGG TGCTGCAGGA TGCCTGCTGG 
AATCTTCTGG AGGGATCACA TCTCCCAGTG TCATGTGACT CTCTTCCTGG GAAGAATTCT 
GGGGTTTCTT GTCTTTATAT TCATTTTTAT GCAGGTCCTA AGACACAAGT TTCCGCTTCT 
TCGGAGCTGA CAGACAACTT CAACCCAGAA GTTGGCCTCT TGCAGTTGAT TGGAGAACCT 
GTACCTGAAC AGATCACCAA GGTCTTTGGG CCCGACAAGA CTCCCGCCTA TGTATTTGGT 
AAAGAAGCCA ATACAGGCCA GGTGGCCCAA TACCATCTGC CCAACCCTAT GTATAGGGAC 
TTCTCTCTCC TCTTCCATGT CCAGCCAACC ACAGACAAGG CCGGCATCCT CTTTGCCATC 
ACAGATGCCT CTCAGTCTAT TGTGTATATC GGGGTGAAGC TTTCTGAAGT CAAGGATGGG 
AAGCAAGACA TCATCTTCTA CTATACAGAG CCTGGCTCAC AGGATTCCTA TGTAGCTGCC 
ACCTTCAATG TCCCATCACT GGTGAATCGT TGGTCTCGCT TTGCAATCAG TGTGGAGGAA 
GAAGAAGTTG TGCTGTTTAT GGATTGCGAT GAGCTGGAAA GGGTGCGCTT CGAGAGATCA 
CCTGATGAGA TGGAACTAGA AGATGGCTCT GGGCTCTTTG TTGCTCAAGC AGGTGGCGCT 
GATCCAGACA GATATCAGGG TGTAATAGCA GATCTAAAGA TCGTAGCCGA TCCAAGAGCC 
GCAGAACATC AGTGTGAGGA GGATGGAGAT GACTCTGATG GGATCTCTGG AGATTTTGGC 
AGTGGCATTG CGGACAATGA TTTGGTGACT TCTCCTCCTG TGATAATCAA AGTGCAGCCT 
CCTATAACTG TCCCTCCCAT ACAGAGGACC CCTGTGGAGA AAGAGATTGT TCAGACAGGT 
CAAGGCCAGC AAGGGGTAAA AGGAGAGAAG GGTGATCGTG GAGAGAAGGG TGAGAGGGGA 
CTGACTGGCC CTAAGGGAGA CGGTGGGAAT GCAAGTCCAA CTTCTGATTC CAAAGGAGAA 
AAGGGTCAGA AAGGAGAGGT CGGAGTGAAG GGAAGTGCAG GCTTTGGCTA TCCCGGGCCT 
AAAGGGCAGA AAGGAGATCC TGGAGACTTG GGACCAAGAG GACCACCAGG CCCACCTGGA 
CCATCTATAT TACAGAAAAG TGATGGAACT ACAGTGCAAC AGGTCACAGG ACCTCCAGGT 
CCTCAAGGCC CTGCGGGACC ACCAGGAAAA GAAGGGGAAC CAGGTGACCC TGGTGAAGAT 
GGAAAAACTG GCGAGGTTGG TCCCCAAGGA TTTCCAGGGA CCCCAGGAGA TTCTGGGCAG 
AAGGGAGACA AGGGTGATTC TGGTGTTGGT ATCAGAGGTG CTCCTGGGCC ACCAGGTCCG 
CCAGGCCCCC CTGGACCTTC TAGAACCGAC AAGCTGACAT TCATTGACAT GGAAGGTTCT 
GGATATGGTG GAGATTCGGA GCACATCAGG GGAGCCCCTG GTGTGCCTGG GCCCCCAGGT 
CCTCCAGGTG TACCAGGGTT ACCCGGAGTA CCCGGAACTT TTGGCATGAA CAACACAGGA 
TTGCCAGGTC CACCTGGATT GCCAGGTCTT CCCGGAAAGG AAGGAATTCC AGGTCCTCCT 
GGCCCCCCAG GACTCCCTGG AAAAGACGGG TTGTCTGGTA CACCTGGTGC TGCTGGATTA 
CGGGGTGAAG ATGGAGAGAT TGGTCTTCCA GGTGCTCCTG GAACAAAGGG TAGCACTGGT 
GAGCCAGGAC TACCTGGACC TCCTGGACAA TCTGGTTTAG CTGGACTTCC TGGACCAATG 
GGACCACGCG GGCTGCCAGG TCCTCCAGGA CCACCTGGGC CTGTGGGTCA TGGATCTGCA 
ATTGGCTTTG ATGACATGGA AGCCTCAGGG CTTGGATTTG GCTTGAGAGA AGGGACACAG 
GGGGTGCCTG GACTTCCCGG GGCAAAGGGA GAGCCAGGAC ATCCAGGGCT GCCAGGAGTG 
AAGGGAGATA GTGGTTTTCC AGGCCTTGAA GGAAGGCCGG GGTTGGATGG ATTCCCAGGT 
CCAGAGGGAT TAAAGGGTGA GAAAGGTGAA CGTGGTGAGC GGGGAGAATC TGGCCGTGAT 
GGTGTTGGTA TTCCAGGTCC ACCGGGACCT CCTGGTCAGG TGCTTTATTT ACCATCCGAT 
GACAAGACTA TTGTTGGTGC CATTCCTGGA CCAGAGGGCA GGCAAGGTGT AGCTGGACTT 
CCGGGCCCAA GGGGACCCAA AGGAGAGGCG GGCTTGAACG GTATCCCAGG AACCTTTGGA 
GTAAAGGGAG AGAAGGGAGA GCCTGGAATT ATTATGGGAC CAGATAGCAC CATTTACACT 
GATTCATCTA AAGGAGAGAA GGGTGACGAA GGACCAAGGG GACCAGCAGG AGCTGCGGGA 
CCATTTGGAC AAAAGGGAGA AATCGGGTTC CCAGGACGCC CTGGACGTCC TGGTATGAAT 
GGACTGAAAG GGGAGAAAGG AGAACCTAGT GGTGCAAGTG GTGGCTTTGG ACTGAGGGGG 
CCACCTGGTC CTCCTGGCCC ACCCGGTCCT CCTGGCAGTG TGGTGTATGG CGATCCAAAT 
AACCCAGGAG CCCAAGTATA TCCAGGTCAT AAAGGGGAGA GAGGATTTCC TGGACCCAAA 
GGTGATGTTG GTCCTGCTGG CCCACCAGGC TCTTTCCCAT ATGATTTAAG TACCTTTGCC 
TCGATTTTAA GGAGAGAAGC TGGACTCAAA GGAGAGAAGG GCGAATCTGG GGGTGGTTCC 
CATGGACAGG GGATCCCAGG GCCACCTGGA AACCCTGGAT ATCCAGGACT GCCGGGAGCT 
AAAGGAGACA GTGTTCAAGG TCCTCCAGGC CCAGCCGGCC CACCAGGACC TTCTGGAATC 
GGTTATGAAG GAAGGCAAGG TCCACCAGGG CCCCCAGGTC CTCCAGGCCT GCCAGGTTCC 
TCCTCTTATC CTGGGCCTCA CAGGCAAACC ATCAGCATAC CTGGACCTCC TGGCCCACCA 
GGCCCACCAG GGCTCCCAGG AACATCAGGA GGATTATCTG GGGCTACTGT GCTACAGACC 
TATCAGACTA TGATCAACTT AGCCCACAGG CTGCAGGAAG GGACAATTGT GCTGGTCCAG 
GAGAGGTCGG AACTGTATGT GCGTGTGAGA GAAGGCTTCC GAAAAATTCT TCTTGGTGAA 
TATACAACAT CCTCTGGCCC TGGACAGGAT AATGAAGTTG CTGCGGAGCA GGCCCCTGTA 
GTTCCATATC CACACCGTAA CAAAGATTCT GATTACCTCC AGTCTACAGA GGAGAACAGC 
AACAATCCAA ATGTGCCCTC AGGTGCCCGA CCCTGGAGCG ATCCTCGGTT ACCAGAACCA 
CCAAGAGTAA ACCCACCACC ACCCCCAGAC CATCACCACC ATGGAAACCC TCGTCCATTG 
GAACCATCCC CAGTGCACAC ACACCAAGAG TTTAATCCTG TGCTTCACCT TGTGGCCCTG 
AACACTCCAC TGAGTGGCAG CATGAAATCC ATCCGCGGAG TGGACTTCCA GTGCTTTGAG 
CAAGCCCGTA AAGCTGGACT ACATGGCACA TTTAGAGCGT TTTTGTCTTC CCGACTTCAA 
GACCTGTACA GCATTGTACG CAGAGCTGAT CGACAATCTG TGCAAATTGT TAATCTTAGG 
GATGAAGTGT TATTTGACAA CTGGGAGTCC CTGTTCTCTG GTTCTGAGGC TCAGATGAGG 
CCTGGTGCGA GTATCTTCTC TTTTGATGGA AAAGATGTTG CAACAGATCC TACATGGCCA 
CAGAAGATGG TTTGGCATGG GTCTGACTCG AAGGGACGGC GGCTTACTGA AAGCTATTGC 
GAGACCTGGC GCACAGATGA AGGTGCAGTA ACTGGTCAGG CATCATCGCT GACGAGTGGG 
AAACTCTTAG AACAGCGCCC ACAGAGTTGC AACAAGAACT TCATTGTTTT GTGTATTGAG 
AACAGCTTCA TGACCAATAA CCGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826359.1
CDS	168..4757
Translation	MRISIPQVTSLSSGLSHDVLNSSQLNGNNIGKQSTYTTNQVYKSPELLKHSGIAATLHGVSAPNNYVTSLTGHPHPSETDLLTSTKSPIPSFSHNSSRTAFILPRLTPSNSRCLPTPTSLSFCKRLGIDSFLLPNYLNHSTVLEIQAALHDWEGLFKSQCHRYLESFFCLLLAPSCNAGTVAPPLVPCRQFCEVLQDACWNLLEGSHLPVSCDSLPGKNSGVSCLYIHFYAGPKTQVSASSELTDNFNPEVGLLQLIGEPVPEQITKVFGPDKTPAYVFGKEANTGQVAQYHLPNPMYRDFSLLFHVQPTTDKAGILFAITDASQSIVYIGVKLSEVKDGKQDIIFYYTEPGSQDSYVAATFNVPSLVNRWSRFAISVEEEEVVLFMDCDELERVRFERSPDEMELEDGSGLFVAQAGGADPDRYQGVIADLKIVADPRAAEHQCEEDGDDSDGISGDFGSGIADNDLVTSPPVIIKVQPPITVPPIQRTPVEKEIVQTGQGQQGVKGEKGDRGEKGERGLTGPKGDGGNASPTSDSKGEKGQKGEVGVKGSAGFGYPGPKGQKGDPGDLGPRGPPGPPGPSILQKSDGTTVQQVTGPPGPQGPAGPPGKEGEPGDPGEDGKTGEVGPQGFPGTPGDSGQKGDKGDSGVGIRGAPGPPGPPGPPGPSRTDKLTFIDMEGSGYGGDSEHIRGAPGVPGPPGPPGVPGLPGVPGTFGMNNTGLPGPPGLPGLPGKEGIPGPPGPPGLPGKDGLSGTPGAAGLRGEDGEIGLPGAPGTKGSTGEPGLPGPPGQSGLAGLPGPMGPRGLPGPPGPPGPVGHGSAIGFDDMEASGLGFGLREGTQGVPGLPGAKGEPGHPGLPGVKGDSGFPGLEGRPGLDGFPGPEGLKGEKGERGERGESGRDGVGIPGPPGPPGQVLYLPSDDKTIVGAIPGPEGRQGVAGLPGPRGPKGEAGLNGIPGTFGVKGEKGEPGIIMGPDSTIYTDSSKGEKGDEGPRGPAGAAGPFGQKGEIGFPGRPGRPGMNGLKGEKGEPSGASGGFGLRGPPGPPGPPGPPGSVVYGDPNNPGAQVYPGHKGERGFPGPKGDVGPAGPPGSFPYDLSTFASILRREAGLKGEKGESGGGSHGQGIPGPPGNPGYPGLPGAKGDSVQGPPGPAGPPGPSGIGYEGRQGPPGPPGPPGLPGSSSYPGPHRQTISIPGPPGPPGPPGLPGTSGGLSGATVLQTYQTMINLAHRLQEGTIVLVQERSELYVRVREGFRKILLGEYTTSSGPGQDNEVAAEQAPVVPYPHRNKDSDYLQSTEENSNNPNVPSGARPWSDPRLPEPPRVNPPPPPDHHHHGNPRPLEPSPVHTHQEFNPVLHLVALNTPLSGSMKSIRGVDFQCFEQARKAGLHGTFRAFLSSRLQDLYSIVRRADRQSVQIVNLRDEVLFDNWESLFSGSEAQMRPGASIFSFDGKDVATDPTWPQKMVWHGSDSKGRRLTESYCETWRTDEGAVTGQASSLTSGKLLEQRPQSCNKNFIVLCIENSFMTNNRK

Target ORF information:

RefSeq Version	XM_012970905.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type XVIII, alpha 1 (col18a1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970905.1

ORF Insert Sequence:

ATGCGCATTT CCATTCCACA AGTAACCTCT CTTTCCAGTG GCCTATCCCA TGATGTGTTA 
AATTCATCCC AGTTGAATGG GAATAACATA GGCAAACAGA GCACTTACAC CACCAATCAG 
GTTTATAAGA GCCCTGAGCT CCTGAAACAT TCTGGGATAG CTGCCACTCT TCATGGTGTT 
TCTGCACCTA ACAATTATGT CACTAGTCTA ACGGGACATC CCCATCCATC TGAAACTGAT 
TTACTAACCA GCACTAAATC TCCCATTCCA TCTTTTAGCC ATAATAGCTC CAGGACTGCT 
TTTATCTTGC CCAGATTGAC TCCATCAAAT TCTCGCTGCT TGCCTACCCC TACCAGCCTC 
TCATTCTGCA AAAGATTAGG AATTGACTCC TTTCTGCTCC CCAATTATCT GAACCATAGT 
ACTGTGCTGG AAATTCAGGC AGCTTTGCAT GATTGGGAGG GGCTGTTTAA ATCTCAGTGC 
CACCGCTACC TGGAATCGTT TTTCTGCTTG CTTCTGGCAC CAAGTTGTAA TGCTGGCACT 
GTGGCACCTC CGCTGGTGCC ATGCCGTCAG TTTTGTGAGG TGCTGCAGGA TGCCTGCTGG 
AATCTTCTGG AGGGATCACA TCTCCCAGTG TCATGTGACT CTCTTCCTGG GAAGAATTCT 
GGGGTTTCTT GTCTTTATAT TCATTTTTAT GCAGGTCCTA AGACACAAGT TTCCGCTTCT 
TCGGAGCTGA CAGACAACTT CAACCCAGAA GTTGGCCTCT TGCAGTTGAT TGGAGAACCT 
GTACCTGAAC AGATCACCAA GGTCTTTGGG CCCGACAAGA CTCCCGCCTA TGTATTTGGT 
AAAGAAGCCA ATACAGGCCA GGTGGCCCAA TACCATCTGC CCAACCCTAT GTATAGGGAC 
TTCTCTCTCC TCTTCCATGT CCAGCCAACC ACAGACAAGG CCGGCATCCT CTTTGCCATC 
ACAGATGCCT CTCAGTCTAT TGTGTATATC GGGGTGAAGC TTTCTGAAGT CAAGGATGGG 
AAGCAAGACA TCATCTTCTA CTATACAGAG CCTGGCTCAC AGGATTCCTA TGTAGCTGCC 
ACCTTCAATG TCCCATCACT GGTGAATCGT TGGTCTCGCT TTGCAATCAG TGTGGAGGAA 
GAAGAAGTTG TGCTGTTTAT GGATTGCGAT GAGCTGGAAA GGGTGCGCTT CGAGAGATCA 
CCTGATGAGA TGGAACTAGA AGATGGCTCT GGGCTCTTTG TTGCTCAAGC AGGTGGCGCT 
GATCCAGACA GATATCAGGG TGTAATAGCA GATCTAAAGA TCGTAGCCGA TCCAAGAGCC 
GCAGAACATC AGTGTGAGGA GGATGGAGAT GACTCTGATG GGATCTCTGG AGATTTTGGC 
AGTGGCATTG CGGACAATGA TTTGGTGACT TCTCCTCCTG TGATAATCAA AGTGCAGCCT 
CCTATAACTG TCCCTCCCAT ACAGAGGACC CCTGTGGAGA AAGAGATTGT TCAGACAGGT 
CAAGGCCAGC AAGGGGTAAA AGGAGAGAAG GGTGATCGTG GAGAGAAGGG TGAGAGGGGA 
CTGACTGGCC CTAAGGGAGA CGGTGGGAAT GCAAGTCCAA CTTCTGATTC CAAAGGAGAA 
AAGGGTCAGA AAGGAGAGGT CGGAGTGAAG GGAAGTGCAG GCTTTGGCTA TCCCGGGCCT 
AAAGGGCAGA AAGGAGATCC TGGAGACTTG GGACCAAGAG GACCACCAGG CCCACCTGGA 
CCATCTATAT TACAGAAAAG TGATGGAACT ACAGTGCAAC AGGTCACAGG ACCTCCAGGT 
CCTCAAGGCC CTGCGGGACC ACCAGGAAAA GAAGGGGAAC CAGGTGACCC TGGTGAAGAT 
GGAAAAACTG GCGAGGTTGG TCCCCAAGGA TTTCCAGGGA CCCCAGGAGA TTCTGGGCAG 
AAGGGAGACA AGGGTGATTC TGGTGTTGGT ATCAGAGGTG CTCCTGGGCC ACCAGGTCCG 
CCAGGCCCCC CTGGACCTTC TAGAACCGAC AAGCTGACAT TCATTGACAT GGAAGGTTCT 
GGATATGGTG GAGATTCGGA GCACATCAGG GGAGCCCCTG GTGTGCCTGG GCCCCCAGGT 
CCTCCAGGTG TACCAGGGTT ACCCGGAGTA CCCGGAACTT TTGGCATGAA CAACACAGGA 
TTGCCAGGTC CACCTGGATT GCCAGGTCTT CCCGGAAAGG AAGGAATTCC AGGTCCTCCT 
GGCCCCCCAG GACTCCCTGG AAAAGACGGG TTGTCTGGTA CACCTGGTGC TGCTGGATTA 
CGGGGTGAAG ATGGAGAGAT TGGTCTTCCA GGTGCTCCTG GAACAAAGGG TAGCACTGGT 
GAGCCAGGAC TACCTGGACC TCCTGGACAA TCTGGTTTAG CTGGACTTCC TGGACCAATG 
GGACCACGCG GGCTGCCAGG TCCTCCAGGA CCACCTGGGC CTGTGGGTCA TGGATCTGCA 
ATTGGCTTTG ATGACATGGA AGCCTCAGGG CTTGGATTTG GCTTGAGAGA AGGGACACAG 
GGGGTGCCTG GACTTCCCGG GGCAAAGGGA GAGCCAGGAC ATCCAGGGCT GCCAGGAGTG 
AAGGGAGATA GTGGTTTTCC AGGCCTTGAA GGAAGGCCGG GGTTGGATGG ATTCCCAGGT 
CCAGAGGGAT TAAAGGGTGA GAAAGGTGAA CGTGGTGAGC GGGGAGAATC TGGCCGTGAT 
GGTGTTGGTA TTCCAGGTCC ACCGGGACCT CCTGGTCAGG TGCTTTATTT ACCATCCGAT 
GACAAGACTA TTGTTGGTGC CATTCCTGGA CCAGAGGGCA GGCAAGGTGT AGCTGGACTT 
CCGGGCCCAA GGGGACCCAA AGGAGAGGCG GGCTTGAACG GTATCCCAGG AACCTTTGGA 
GTAAAGGGAG AGAAGGGAGA GCCTGGAATT ATTATGGGAC CAGATAGCAC CATTTACACT 
GATTCATCTA AAGGAGAGAA GGGTGACGAA GGACCAAGGG GACCAGCAGG AGCTGCGGGA 
CCATTTGGAC AAAAGGGAGA AATCGGGTTC CCAGGACGCC CTGGACGTCC TGGTATGAAT 
GGACTGAAAG GGGAGAAAGG AGAACCTAGT GGTGCAAGTG GTGGCTTTGG ACTGAGGGGG 
CCACCTGGTC CTCCTGGCCC ACCCGGTCCT CCTGGCAGTG TGGTGTATGG CGATCCAAAT 
AACCCAGGAG CCCAAGTATA TCCAGGTCAT AAAGGGGAGA GAGGATTTCC TGGACCCAAA 
GGTGATGTTG GTCCTGCTGG CCCACCAGGC TCTTTCCCAT ATGATTTAAG TACCTTTGCC 
TCGATTTTAA GGAGAGAAGC TGGACTCAAA GGAGAGAAGG GCGAATCTGG GGGTGGTTCC 
CATGGACAGG GGATCCCAGG GCCACCTGGA AACCCTGGAT ATCCAGGACT GCCGGGAGCT 
AAAGGAGACA GTGTTCAAGG TCCTCCAGGC CCAGCCGGCC CACCAGGACC TTCTGGAATC 
GGTTATGAAG GAAGGCAAGG TCCACCAGGG CCCCCAGGTC CTCCAGGCCT GCCAGGTTCC 
TCCTCTTATC CTGGGCCTCA CAGGCAAACC ATCAGCATAC CTGGACCTCC TGGCCCACCA 
GGCCCACCAG GGCTCCCAGG AACATCAGGA GGATTATCTG GGGCTACTGT GCTACAGACC 
TATCAGACTA TGATCAACTT AGCCCACAGG CTGCAGGAAG GGACAATTGT GCTGGTCCAG 
GAGAGGTCGG AACTGTATGT GCGTGTGAGA GAAGGCTTCC GAAAAATTCT TCTTGGTGAA 
TATACAACAT CCTCTGGCCC TGGACAGGAT AATGAAGTTG CTGCGGAGCA GGCCCCTGTA 
GTTCCATATC CACACCGTAA CAAAGATTCT GATTACCTCC AGTCTACAGA GGAGAACAGC 
AACAATCCAA ATGTGCCCTC AGGTGCCCGA CCCTGGAGCG ATCCTCGGTT ACCAGAACCA 
CCAAGAGTAA ACCCACCACC ACCCCCAGAC CATCACCACC ATGGAAACCC TCGTCCATTG 
GAACCATCCC CAGTGCACAC ACACCAAGAG TTTAATCCTG TGCTTCACCT TGTGGCCCTG 
AACACTCCAC TGAGTGGCAG CATGAAATCC ATCCGCGGAG TGGACTTCCA GTGCTTTGAG 
CAAGCCCGTA AAGCTGGACT ACATGGCACA TTTAGAGCGT TTTTGTCTTC CCGACTTCAA 
GACCTGTACA GCATTGTACG CAGAGCTGAT CGACAATCTG TGCAAATTGT TAATCTTAGG 
GATGAAGTGT TATTTGACAA CTGGGAGTCC CTGTTCTCTG GTTCTGAGGC TCAGATGAGG 
CCTGGTGCGA GTATCTTCTC TTTTGATGGA AAAGATGTTG CAACAGATCC TACATGGCCA 
CAGAAGATGG TTTGGCATGG GTCTGACTCG AAGGGACGGC GGCTTACTGA AAGCTATTGC 
GAGACCTGGC GCACAGATGA AGGTGCAGTA ACTGGTCAGG CATCATCGCT GACGAGTGGG 
AAACTCTTAG AACAGCGCCC ACAGAGTTGC AACAAGAACT TCATTGTTTT GTGTATTGAG 
AACAGCTTCA TGACCAATAA CCGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col18a1 ( XM_012970906.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826360.1 Xenopus tropicalis collagen, type XVIII, alpha 1 (col18a1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa26100
Clone ID Related Accession (Same CDS sequence)	XM_012970906.2
Accession Version	XM_012970906.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(XVIII) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970906.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCTCA GGTGTACGTG GGTCCTGTTG CTCTACGCAT CGTATCATGT TCTTCTGGTC 
TCCTCCCAAG GCAAAGACAA CTTCAACCCA GAAGTTGGCC TCTTGCAGTT GATTGGAGAA 
CCTGTACCTG AACAGATCAC CAAGGTCTTT GGGCCCGACA AGACTCCCGC CTATGTATTT 
GGTAAAGAAG CCAATACAGG CCAGGTGGCC CAATACCATC TGCCCAACCC TATGTATAGG 
GACTTCTCTC TCCTCTTCCA TGTCCAGCCA ACCACAGACA AGGCCGGCAT CCTCTTTGCC 
ATCACAGATG CCTCTCAGTC TATTGTGTAT ATCGGGGTGA AGCTTTCTGA AGTCAAGGAT 
GGGAAGCAAG ACATCATCTT CTACTATACA GAGCCTGGCT CACAGGATTC CTATGTAGCT 
GCCACCTTCA ATGTCCCATC ACTGGTGAAT CGTTGGTCTC GCTTTGCAAT CAGTGTGGAG 
GAAGAAGAAG TTGTGCTGTT TATGGATTGC GATGAGCTGG AAAGGGTGCG CTTCGAGAGA 
TCACCTGATG AGATGGAACT AGAAGATGGC TCTGGGCTCT TTGTTGCTCA AGCAGGTGGC 
GCTGATCCAG ACAGATATCA GGGTGTAATA GCAGATCTAA AGATCGTAGC CGATCCAAGA 
GCCGCAGAAC ATCAGTGTGA GGAGGATGGA GATGACTCTG ATGGGATCTC TGGAGATTTT 
GGCAGTGGCA TTGCGGACAA TGATTTGGTG ACTTCTCCTC CTGTGATAAT CAAAGTGCAG 
CCTCCTATAA CTGTCCCTCC CATACAGAGG ACCCCTGTGG AGAAAGAGAT TGTTCAGACA 
GGTCAAGGCC AGCAAGGGGT AAAAGGAGAG AAGGGTGATC GTGGAGAGAA GGGTGAGAGG 
GGACTGACTG GCCCTAAGGG AGACGGTGGG AATGCAAGTC CAACTTCTGA TTCCAAAGGA 
GAAAAGGGTC AGAAAGGAGA GGTCGGAGTG AAGGGAAGTG CAGGCTTTGG CTATCCCGGG 
CCTAAAGGGC AGAAAGGAGA TCCTGGAGAC TTGGGACCAA GAGGACCACC AGGCCCACCT 
GGACCATCTA TATTACAGAA AAGTGATGGA ACTACAGTGC AACAGGTCAC AGGACCTCCA 
GGTCCTCAAG GCCCTGCGGG ACCACCAGGA AAAGAAGGGG AACCAGGTGA CCCTGGTGAA 
GATGGAAAAA CTGGCGAGGT TGGTCCCCAA GGATTTCCAG GGACCCCAGG AGATTCTGGG 
CAGAAGGGAG ACAAGGGTGA TTCTGGTGTT GGTATCAGAG GTGCTCCTGG GCCACCAGGT 
CCGCCAGGCC CCCCTGGACC TTCTAGAACC GACAAGCTGA CATTCATTGA CATGGAAGGT 
TCTGGATATG GTGGAGATTC GGAGCACATC AGGGGAGCCC CTGGTGTGCC TGGGCCCCCA 
GGTCCTCCAG GTGTACCAGG GTTACCCGGA GTACCCGGAA CTTTTGGCAT GAACAACACA 
GGATTGCCAG GTCCACCTGG ATTGCCAGGT CTTCCCGGAA AGGAAGGAAT TCCAGGTCCT 
CCTGGCCCCC CAGGACTCCC TGGAAAAGAC GGGTTGTCTG GTACACCTGG TGCTGCTGGA 
TTACGGGGTG AAGATGGAGA GATTGGTCTT CCAGGTGCTC CTGGAACAAA GGGTAGCACT 
GGTGAGCCAG GACTACCTGG ACCTCCTGGA CAATCTGGTT TAGCTGGACT TCCTGGACCA 
ATGGGACCAC GCGGGCTGCC AGGTCCTCCA GGACCACCTG GGCCTGTGGG TCATGGATCT 
GCAATTGGCT TTGATGACAT GGAAGCCTCA GGGCTTGGAT TTGGCTTGAG AGAAGGGACA 
CAGGGGGTGC CTGGACTTCC CGGGGCAAAG GGAGAGCCAG GACATCCAGG GCTGCCAGGA 
GTGAAGGGAG ATAGTGGTTT TCCAGGCCTT GAAGGAAGGC CGGGGTTGGA TGGATTCCCA 
GGTCCAGAGG GATTAAAGGG TGAGAAAGGT GAACGTGGTG AGCGGGGAGA ATCTGGCCGT 
GATGGTGTTG GTATTCCAGG TCCACCGGGA CCTCCTGGTC AGGTGCTTTA TTTACCATCC 
GATGACAAGA CTATTGTTGG TGCCATTCCT GGACCAGAGG GCAGGCAAGG TGTAGCTGGA 
CTTCCGGGCC CAAGGGGACC CAAAGGAGAG GCGGGCTTGA ACGGTATCCC AGGAACCTTT 
GGAGTAAAGG GAGAGAAGGG AGAGCCTGGA ATTATTATGG GACCAGATAG CACCATTTAC 
ACTGATTCAT CTAAAGGAGA GAAGGGTGAC GAAGGACCAA GGGGACCAGC AGGAGCTGCG 
GGACCATTTG GACAAAAGGG AGAAATCGGG TTCCCAGGAC GCCCTGGACG TCCTGGTATG 
AATGGACTGA AAGGGGAGAA AGGAGAACCT AGTGGTGCAA GTGGTGGCTT TGGACTGAGG 
GGGCCACCTG GTCCTCCTGG CCCACCCGGT CCTCCTGGCA GTGTGGTGTA TGGCGATCCA 
AATAACCCAG GAGCCCAAGT ATATCCAGGT CATAAAGGGG AGAGAGGATT TCCTGGACCC 
AAAGGTGATG TTGGTCCTGC TGGCCCACCA GGCTCTTTCC CATATGATTT AAGTACCTTT 
GCCTCGATTT TAAGGAGAGA AGCTGGACTC AAAGGAGAGA AGGGCGAATC TGGGGGTGGT 
TCCCATGGAC AGGGGATCCC AGGGCCACCT GGAAACCCTG GATATCCAGG ACTGCCGGGA 
GCTAAAGGAG ACAGTGTTCA AGGTCCTCCA GGCCCAGCCG GCCCACCAGG ACCTTCTGGA 
ATCGGTTATG AAGGAAGGCA AGGTCCACCA GGGCCCCCAG GTCCTCCAGG CCTGCCAGGT 
TCCTCCTCTT ATCCTGGGCC TCACAGGCAA ACCATCAGCA TACCTGGACC TCCTGGCCCA 
CCAGGCCCAC CAGGGCTCCC AGGAACATCA GGAGGATTAT CTGGGGCTAC TGTGCTACAG 
ACCTATCAGA CTATGATCAA CTTAGCCCAC AGGCTGCAGG AAGGGACAAT TGTGCTGGTC 
CAGGAGAGGT CGGAACTGTA TGTGCGTGTG AGAGAAGGCT TCCGAAAAAT TCTTCTTGGT 
GAATATACAA CATCCTCTGG CCCTGGACAG GATAATGAAG TTGCTGCGGA GCAGGCCCCT 
GTAGTTCCAT ATCCACACCG TAACAAAGAT TCTGATTACC TCCAGTCTAC AGAGGAGAAC 
AGCAACAATC CAAATGTGCC CTCAGGTGCC CGACCCTGGA GCGATCCTCG GTTACCAGAA 
CCACCAAGAG TAAACCCACC ACCACCCCCA GACCATCACC ACCATGGAAA CCCTCGTCCA 
TTGGAACCAT CCCCAGTGCA CACACACCAA GAGTTTAATC CTGTGCTTCA CCTTGTGGCC 
CTGAACACTC CACTGAGTGG CAGCATGAAA TCCATCCGCG GAGTGGACTT CCAGTGCTTT 
GAGCAAGCCC GTAAAGCTGG ACTACATGGC ACATTTAGAG CGTTTTTGTC TTCCCGACTT 
CAAGACCTGT ACAGCATTGT ACGCAGAGCT GATCGACAAT CTGTGCAAAT TGTTAATCTT 
AGGGATGAAG TGTTATTTGA CAACTGGGAG TCCCTGTTCT CTGGTTCTGA GGCTCAGATG 
AGGCCTGGTG CGAGTATCTT CTCTTTTGAT GGAAAAGATG TTGCAACAGA TCCTACATGG 
CCACAGAAGA TGGTTTGGCA TGGGTCTGAC TCGAAGGGAC GGCGGCTTAC TGAAAGCTAT 
TGCGAGACCT GGCGCACAGA TGAAGGTGCA GTAACTGGTC AGGCATCATC GCTGACGAGT 
GGGAAACTCT TAGAACAGCG CCCACAGAGT TGCAACAAGA ACTTCATTGT TTTGTGTATT 
GAGAACAGCT TCATGACCAA TAACCGGAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826360.1
CDS	178..4110
Translation	MILRCTWVLLLYASYHVLLVSSQGKDNFNPEVGLLQLIGEPVPEQITKVFGPDKTPAYVFGKEANTGQVAQYHLPNPMYRDFSLLFHVQPTTDKAGILFAITDASQSIVYIGVKLSEVKDGKQDIIFYYTEPGSQDSYVAATFNVPSLVNRWSRFAISVEEEEVVLFMDCDELERVRFERSPDEMELEDGSGLFVAQAGGADPDRYQGVIADLKIVADPRAAEHQCEEDGDDSDGISGDFGSGIADNDLVTSPPVIIKVQPPITVPPIQRTPVEKEIVQTGQGQQGVKGEKGDRGEKGERGLTGPKGDGGNASPTSDSKGEKGQKGEVGVKGSAGFGYPGPKGQKGDPGDLGPRGPPGPPGPSILQKSDGTTVQQVTGPPGPQGPAGPPGKEGEPGDPGEDGKTGEVGPQGFPGTPGDSGQKGDKGDSGVGIRGAPGPPGPPGPPGPSRTDKLTFIDMEGSGYGGDSEHIRGAPGVPGPPGPPGVPGLPGVPGTFGMNNTGLPGPPGLPGLPGKEGIPGPPGPPGLPGKDGLSGTPGAAGLRGEDGEIGLPGAPGTKGSTGEPGLPGPPGQSGLAGLPGPMGPRGLPGPPGPPGPVGHGSAIGFDDMEASGLGFGLREGTQGVPGLPGAKGEPGHPGLPGVKGDSGFPGLEGRPGLDGFPGPEGLKGEKGERGERGESGRDGVGIPGPPGPPGQVLYLPSDDKTIVGAIPGPEGRQGVAGLPGPRGPKGEAGLNGIPGTFGVKGEKGEPGIIMGPDSTIYTDSSKGEKGDEGPRGPAGAAGPFGQKGEIGFPGRPGRPGMNGLKGEKGEPSGASGGFGLRGPPGPPGPPGPPGSVVYGDPNNPGAQVYPGHKGERGFPGPKGDVGPAGPPGSFPYDLSTFASILRREAGLKGEKGESGGGSHGQGIPGPPGNPGYPGLPGAKGDSVQGPPGPAGPPGPSGIGYEGRQGPPGPPGPPGLPGSSSYPGPHRQTISIPGPPGPPGPPGLPGTSGGLSGATVLQTYQTMINLAHRLQEGTIVLVQERSELYVRVREGFRKILLGEYTTSSGPGQDNEVAAEQAPVVPYPHRNKDSDYLQSTEENSNNPNVPSGARPWSDPRLPEPPRVNPPPPPDHHHHGNPRPLEPSPVHTHQEFNPVLHLVALNTPLSGSMKSIRGVDFQCFEQARKAGLHGTFRAFLSSRLQDLYSIVRRADRQSVQIVNLRDEVLFDNWESLFSGSEAQMRPGASIFSFDGKDVATDPTWPQKMVWHGSDSKGRRLTESYCETWRTDEGAVTGQASSLTSGKLLEQRPQSCNKNFIVLCIENSFMTNNRK

Target ORF information:

RefSeq Version	XM_012970906.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type XVIII, alpha 1 (col18a1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970906.2

ORF Insert Sequence:

ATGATTCTCA GGTGTACGTG GGTCCTGTTG CTCTACGCAT CGTATCATGT TCTTCTGGTC 
TCCTCCCAAG GCAAAGACAA CTTCAACCCA GAAGTTGGCC TCTTGCAGTT GATTGGAGAA 
CCTGTACCTG AACAGATCAC CAAGGTCTTT GGGCCCGACA AGACTCCCGC CTATGTATTT 
GGTAAAGAAG CCAATACAGG CCAGGTGGCC CAATACCATC TGCCCAACCC TATGTATAGG 
GACTTCTCTC TCCTCTTCCA TGTCCAGCCA ACCACAGACA AGGCCGGCAT CCTCTTTGCC 
ATCACAGATG CCTCTCAGTC TATTGTGTAT ATCGGGGTGA AGCTTTCTGA AGTCAAGGAT 
GGGAAGCAAG ACATCATCTT CTACTATACA GAGCCTGGCT CACAGGATTC CTATGTAGCT 
GCCACCTTCA ATGTCCCATC ACTGGTGAAT CGTTGGTCTC GCTTTGCAAT CAGTGTGGAG 
GAAGAAGAAG TTGTGCTGTT TATGGATTGC GATGAGCTGG AAAGGGTGCG CTTCGAGAGA 
TCACCTGATG AGATGGAACT AGAAGATGGC TCTGGGCTCT TTGTTGCTCA AGCAGGTGGC 
GCTGATCCAG ACAGATATCA GGGTGTAATA GCAGATCTAA AGATCGTAGC CGATCCAAGA 
GCCGCAGAAC ATCAGTGTGA GGAGGATGGA GATGACTCTG ATGGGATCTC TGGAGATTTT 
GGCAGTGGCA TTGCGGACAA TGATTTGGTG ACTTCTCCTC CTGTGATAAT CAAAGTGCAG 
CCTCCTATAA CTGTCCCTCC CATACAGAGG ACCCCTGTGG AGAAAGAGAT TGTTCAGACA 
GGTCAAGGCC AGCAAGGGGT AAAAGGAGAG AAGGGTGATC GTGGAGAGAA GGGTGAGAGG 
GGACTGACTG GCCCTAAGGG AGACGGTGGG AATGCAAGTC CAACTTCTGA TTCCAAAGGA 
GAAAAGGGTC AGAAAGGAGA GGTCGGAGTG AAGGGAAGTG CAGGCTTTGG CTATCCCGGG 
CCTAAAGGGC AGAAAGGAGA TCCTGGAGAC TTGGGACCAA GAGGACCACC AGGCCCACCT 
GGACCATCTA TATTACAGAA AAGTGATGGA ACTACAGTGC AACAGGTCAC AGGACCTCCA 
GGTCCTCAAG GCCCTGCGGG ACCACCAGGA AAAGAAGGGG AACCAGGTGA CCCTGGTGAA 
GATGGAAAAA CTGGCGAGGT TGGTCCCCAA GGATTTCCAG GGACCCCAGG AGATTCTGGG 
CAGAAGGGAG ACAAGGGTGA TTCTGGTGTT GGTATCAGAG GTGCTCCTGG GCCACCAGGT 
CCGCCAGGCC CCCCTGGACC TTCTAGAACC GACAAGCTGA CATTCATTGA CATGGAAGGT 
TCTGGATATG GTGGAGATTC GGAGCACATC AGGGGAGCCC CTGGTGTGCC TGGGCCCCCA 
GGTCCTCCAG GTGTACCAGG GTTACCCGGA GTACCCGGAA CTTTTGGCAT GAACAACACA 
GGATTGCCAG GTCCACCTGG ATTGCCAGGT CTTCCCGGAA AGGAAGGAAT TCCAGGTCCT 
CCTGGCCCCC CAGGACTCCC TGGAAAAGAC GGGTTGTCTG GTACACCTGG TGCTGCTGGA 
TTACGGGGTG AAGATGGAGA GATTGGTCTT CCAGGTGCTC CTGGAACAAA GGGTAGCACT 
GGTGAGCCAG GACTACCTGG ACCTCCTGGA CAATCTGGTT TAGCTGGACT TCCTGGACCA 
ATGGGACCAC GCGGGCTGCC AGGTCCTCCA GGACCACCTG GGCCTGTGGG TCATGGATCT 
GCAATTGGCT TTGATGACAT GGAAGCCTCA GGGCTTGGAT TTGGCTTGAG AGAAGGGACA 
CAGGGGGTGC CTGGACTTCC CGGGGCAAAG GGAGAGCCAG GACATCCAGG GCTGCCAGGA 
GTGAAGGGAG ATAGTGGTTT TCCAGGCCTT GAAGGAAGGC CGGGGTTGGA TGGATTCCCA 
GGTCCAGAGG GATTAAAGGG TGAGAAAGGT GAACGTGGTG AGCGGGGAGA ATCTGGCCGT 
GATGGTGTTG GTATTCCAGG TCCACCGGGA CCTCCTGGTC AGGTGCTTTA TTTACCATCC 
GATGACAAGA CTATTGTTGG TGCCATTCCT GGACCAGAGG GCAGGCAAGG TGTAGCTGGA 
CTTCCGGGCC CAAGGGGACC CAAAGGAGAG GCGGGCTTGA ACGGTATCCC AGGAACCTTT 
GGAGTAAAGG GAGAGAAGGG AGAGCCTGGA ATTATTATGG GACCAGATAG CACCATTTAC 
ACTGATTCAT CTAAAGGAGA GAAGGGTGAC GAAGGACCAA GGGGACCAGC AGGAGCTGCG 
GGACCATTTG GACAAAAGGG AGAAATCGGG TTCCCAGGAC GCCCTGGACG TCCTGGTATG 
AATGGACTGA AAGGGGAGAA AGGAGAACCT AGTGGTGCAA GTGGTGGCTT TGGACTGAGG 
GGGCCACCTG GTCCTCCTGG CCCACCCGGT CCTCCTGGCA GTGTGGTGTA TGGCGATCCA 
AATAACCCAG GAGCCCAAGT ATATCCAGGT CATAAAGGGG AGAGAGGATT TCCTGGACCC 
AAAGGTGATG TTGGTCCTGC TGGCCCACCA GGCTCTTTCC CATATGATTT AAGTACCTTT 
GCCTCGATTT TAAGGAGAGA AGCTGGACTC AAAGGAGAGA AGGGCGAATC TGGGGGTGGT 
TCCCATGGAC AGGGGATCCC AGGGCCACCT GGAAACCCTG GATATCCAGG ACTGCCGGGA 
GCTAAAGGAG ACAGTGTTCA AGGTCCTCCA GGCCCAGCCG GCCCACCAGG ACCTTCTGGA 
ATCGGTTATG AAGGAAGGCA AGGTCCACCA GGGCCCCCAG GTCCTCCAGG CCTGCCAGGT 
TCCTCCTCTT ATCCTGGGCC TCACAGGCAA ACCATCAGCA TACCTGGACC TCCTGGCCCA 
CCAGGCCCAC CAGGGCTCCC AGGAACATCA GGAGGATTAT CTGGGGCTAC TGTGCTACAG 
ACCTATCAGA CTATGATCAA CTTAGCCCAC AGGCTGCAGG AAGGGACAAT TGTGCTGGTC 
CAGGAGAGGT CGGAACTGTA TGTGCGTGTG AGAGAAGGCT TCCGAAAAAT TCTTCTTGGT 
GAATATACAA CATCCTCTGG CCCTGGACAG GATAATGAAG TTGCTGCGGA GCAGGCCCCT 
GTAGTTCCAT ATCCACACCG TAACAAAGAT TCTGATTACC TCCAGTCTAC AGAGGAGAAC 
AGCAACAATC CAAATGTGCC CTCAGGTGCC CGACCCTGGA GCGATCCTCG GTTACCAGAA 
CCACCAAGAG TAAACCCACC ACCACCCCCA GACCATCACC ACCATGGAAA CCCTCGTCCA 
TTGGAACCAT CCCCAGTGCA CACACACCAA GAGTTTAATC CTGTGCTTCA CCTTGTGGCC 
CTGAACACTC CACTGAGTGG CAGCATGAAA TCCATCCGCG GAGTGGACTT CCAGTGCTTT 
GAGCAAGCCC GTAAAGCTGG ACTACATGGC ACATTTAGAG CGTTTTTGTC TTCCCGACTT 
CAAGACCTGT ACAGCATTGT ACGCAGAGCT GATCGACAAT CTGTGCAAAT TGTTAATCTT 
AGGGATGAAG TGTTATTTGA CAACTGGGAG TCCCTGTTCT CTGGTTCTGA GGCTCAGATG 
AGGCCTGGTG CGAGTATCTT CTCTTTTGAT GGAAAAGATG TTGCAACAGA TCCTACATGG 
CCACAGAAGA TGGTTTGGCA TGGGTCTGAC TCGAAGGGAC GGCGGCTTAC TGAAAGCTAT 
TGCGAGACCT GGCGCACAGA TGAAGGTGCA GTAACTGGTC AGGCATCATC GCTGACGAGT 
GGGAAACTCT TAGAACAGCG CCCACAGAGT TGCAACAAGA ACTTCATTGT TTTGTGTATT 
GAGAACAGCT TCATGACCAA TAACCGGAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col18a1 ( XM_018097368.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952857.1 Xenopus tropicalis collagen, type XVIII, alpha 1 (col18a1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa46789
Clone ID Related Accession (Same CDS sequence)	XM_018097368.1
Accession Version	XM_018097368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(XVIII) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATAGGG ACTTCTCTCT CCTCTTCCAT GTCCAGCCAA CCACAGACAA GGCCGGCATC 
CTCTTTGCCA TCACAGATGC CTCTCAGTCT ATTGTGTATA TCGGGGTGAA GCTTTCTGAA 
GTCAAGGATG GGAAGCAAGA CATCATCTTC TACTATACAG AGCCTGGCTC ACAGGATTCC 
TATGTAGCTG CCACCTTCAA TGTCCCATCA CTGGTGAATC GTTGGTCTCG CTTTGCAATC 
AGTGTGGAGG AAGAAGAAGT TGTGCTGTTT ATGGATTGCG ATGAGCTGGA AAGGGTGCGC 
TTCGAGAGAT CACCTGATGA GATGGAACTA GAAGATGGCT CTGGGCTCTT TGTTGCTCAA 
GCAGGTGGCG CTGATCCAGA CAGATATCAG GGTGTAATAG CAGATCTAAA GATCGTAGCC 
GATCCAAGAG CCGCAGAACA TCAGTGTGAG GAGGATGGAG ATGACTCTGA TGGGATCTCT 
GGAGATTTTG GCAGTGGCAT TGCGGACAAT GATTTGGTGA CTTCTCCTCC TGTGATAATC 
AAAGTGCAGC CTCCTATAAC TGTCCCTCCC ATACAGAGGA CCCCTGTGGA GAAAGAGATT 
GTTCAGACAG GTCAAGGCCA GCAAGGGGTA AAAGGAGAGA AGGGTGATCG TGGAGAGAAG 
GGTGAGAGGG GACTGACTGG CCCTAAGGGA GACGGTGGGA ATGCAAGTCC AACTTCTGAT 
TCCAAAGGAG AAAAGGGTCA GAAAGGAGAG GTCGGAGTGA AGGGAAGTGC AGGCTTTGGC 
TATCCCGGGC CTAAAGGGCA GAAAGGAGAT CCTGGAGACT TGGGACCAAG AGGACCACCA 
GGCCCACCTG GACCATCTAT ATTACAGAAA AGTGATGGAA CTACAGTGCA ACAGGTCACA 
GGACCTCCAG GTCCTCAAGG CCCTGCGGGA CCACCAGGAA AAGAAGGGGA ACCAGGTGAC 
CCTGGTGAAG ATGGAAAAAC TGGCGAGGTT GGTCCCCAAG GATTTCCAGG GACCCCAGGA 
GATTCTGGGC AGAAGGGAGA CAAGGGTGAT TCTGGTGTTG GTATCAGAGG TGCTCCTGGG 
CCACCAGGTC CGCCAGGCCC CCCTGGACCT TCTAGAACCG ACAAGCTGAC ATTCATTGAC 
ATGGAAGGTT CTGGATATGG TGGAGATTCG GAGCACATCA GGGGAGCCCC TGGTGTGCCT 
GGGCCCCCAG GTCCTCCAGG TGTACCAGGG TTACCCGGAG TACCCGGAAC TTTTGGCATG 
AACAACACAG GATTGCCAGG TCCACCTGGA TTGCCAGGTC TTCCCGGAAA GGAAGGAATT 
CCAGGTCCTC CTGGCCCCCC AGGACTCCCT GGAAAAGACG GGTTGTCTGG TACACCTGGT 
GCTGCTGGAT TACGGGGTGA AGATGGAGAG ATTGGTCTTC CAGGTGCTCC TGGAACAAAG 
GGTAGCACTG GTGAGCCAGG ACTACCTGGA CCTCCTGGAC AATCTGGTTT AGCTGGACTT 
CCTGGACCAA TGGGACCACG CGGGCTGCCA GGTCCTCCAG GACCACCTGG GCCTGTGGGT 
CATGGATCTG CAATTGGCTT TGATGACATG GAAGCCTCAG GGCTTGGATT TGGCTTGAGA 
GAAGGGACAC AGGGGGTGCC TGGACTTCCC GGGGCAAAGG GAGAGCCAGG ACATCCAGGG 
CTGCCAGGAG TGAAGGGAGA TAGTGGTTTT CCAGGCCTTG AAGGAAGGCC GGGGTTGGAT 
GGATTCCCAG GTCCAGAGGG ATTAAAGGGT GAGAAAGGTG AACGTGGTGA GCGGGGAGAA 
TCTGGCCGTG ATGGTGTTGG TATTCCAGGT CCACCGGGAC CTCCTGGTCA GGTGCTTTAT 
TTACCATCCG ATGACAAGAC TATTGTTGGT GCCATTCCTG GACCAGAGGG CAGGCAAGGT 
GTAGCTGGAC TTCCGGGCCC AAGGGGACCC AAAGGAGAGG CGGGCTTGAA CGGTATCCCA 
GGAACCTTTG GAGTAAAGGG AGAGAAGGGA GAGCCTGGAA TTATTATGGG ACCAGATAGC 
ACCATTTACA CTGATTCATC TAAAGGAGAG AAGGGTGACG AAGGACCAAG GGGACCAGCA 
GGAGCTGCGG GACCATTTGG ACAAAAGGGA GAAATCGGGT TCCCAGGACG CCCTGGACGT 
CCTGGTATGA ATGGACTGAA AGGGGAGAAA GGAGAACCTA GTGGTGCAAG TGGTGGCTTT 
GGACTGAGGG GGCCACCTGG TCCTCCTGGC CCACCCGGTC CTCCTGGCAG TGTGGTGTAT 
GGCGATCCAA ATAACCCAGG AGCCCAAGTA TATCCAGGTC ATAAAGGGGA GAGAGGATTT 
CCTGGACCCA AAGGTGATGT TGGTCCTGCT GGCCCACCAG GCTCTTTCCC ATATGATTTA 
AGTACCTTTG CCTCGATTTT AAGGAGAGAA GCTGGACTCA AAGGAGAGAA GGGCGAATCT 
GGGGGTGGTT CCCATGGACA GGGGATCCCA GGGCCACCTG GAAACCCTGG ATATCCAGGA 
CTGCCGGGAG CTAAAGGAGA CAGTGTTCAA GGTCCTCCAG GCCCAGCCGG CCCACCAGGA 
CCTTCTGGAA TCGGTTATGA AGGAAGGCAA GGTCCACCAG GGCCCCCAGG TCCTCCAGGC 
CTGCCAGGTT CCTCCTCTTA TCCTGGGCCT CACAGGCAAA CCATCAGCAT ACCTGGACCT 
CCTGGCCCAC CAGGCCCACC AGGGCTCCCA GGAACATCAG GAGGATTATC TGGGGCTACT 
GTGCTACAGA CCTATCAGAC TATGATCAAC TTAGCCCACA GGCTGCAGGA AGGGACAATT 
GTGCTGGTCC AGGAGAGGTC GGAACTGTAT GTGCGTGTGA GAGAAGGCTT CCGAAAAATT 
CTTCTTGGTG AATATACAAC ATCCTCTGGC CCTGGACAGG ATAATGAAGT TGCTGCGGAG 
CAGGCCCCTG TAGTTCCATA TCCACACCGT AACAAAGATT CTGATTACCT CCAGTCTACA 
GAGGAGAACA GCAACAATCC AAATGTGCCC TCAGGTGCCC GACCCTGGAG CGATCCTCGG 
TTACCAGAAC CACCAAGAGT AAACCCACCA CCACCCCCAG ACCATCACCA CCATGGAAAC 
CCTCGTCCAT TGGAACCATC CCCAGTGCAC ACACACCAAG AGTTTAATCC TGTGCTTCAC 
CTTGTGGCCC TGAACACTCC ACTGAGTGGC AGCATGAAAT CCATCCGCGG AGTGGACTTC 
CAGTGCTTTG AGCAAGCCCG TAAAGCTGGA CTACATGGCA CATTTAGAGC GTTTTTGTCT 
TCCCGACTTC AAGACCTGTA CAGCATTGTA CGCAGAGCTG ATCGACAATC TGTGCAAATT 
GTTAATCTTA GGGATGAAGT GTTATTTGAC AACTGGGAGT CCCTGTTCTC TGGTTCTGAG 
GCTCAGATGA GGCCTGGTGC GAGTATCTTC TCTTTTGATG GAAAAGATGT TGCAACAGAT 
CCTACATGGC CACAGAAGAT GGTTTGGCAT GGGTCTGACT CGAAGGGACG GCGGCTTACT 
GAAAGCTATT GCGAGACCTG GCGCACAGAT GAAGGTGCAG TAACTGGTCA GGCATCATCG 
CTGACGAGTG GGAAACTCTT AGAACAGCGC CCACAGAGTT GCAACAAGAA CTTCATTGTT 
TTGTGTATTG AGAACAGCTT CATGACCAAT AACCGGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952857.1
CDS	344..4045
Translation	MYRDFSLLFHVQPTTDKAGILFAITDASQSIVYIGVKLSEVKDGKQDIIFYYTEPGSQDSYVAATFNVPSLVNRWSRFAISVEEEEVVLFMDCDELERVRFERSPDEMELEDGSGLFVAQAGGADPDRYQGVIADLKIVADPRAAEHQCEEDGDDSDGISGDFGSGIADNDLVTSPPVIIKVQPPITVPPIQRTPVEKEIVQTGQGQQGVKGEKGDRGEKGERGLTGPKGDGGNASPTSDSKGEKGQKGEVGVKGSAGFGYPGPKGQKGDPGDLGPRGPPGPPGPSILQKSDGTTVQQVTGPPGPQGPAGPPGKEGEPGDPGEDGKTGEVGPQGFPGTPGDSGQKGDKGDSGVGIRGAPGPPGPPGPPGPSRTDKLTFIDMEGSGYGGDSEHIRGAPGVPGPPGPPGVPGLPGVPGTFGMNNTGLPGPPGLPGLPGKEGIPGPPGPPGLPGKDGLSGTPGAAGLRGEDGEIGLPGAPGTKGSTGEPGLPGPPGQSGLAGLPGPMGPRGLPGPPGPPGPVGHGSAIGFDDMEASGLGFGLREGTQGVPGLPGAKGEPGHPGLPGVKGDSGFPGLEGRPGLDGFPGPEGLKGEKGERGERGESGRDGVGIPGPPGPPGQVLYLPSDDKTIVGAIPGPEGRQGVAGLPGPRGPKGEAGLNGIPGTFGVKGEKGEPGIIMGPDSTIYTDSSKGEKGDEGPRGPAGAAGPFGQKGEIGFPGRPGRPGMNGLKGEKGEPSGASGGFGLRGPPGPPGPPGPPGSVVYGDPNNPGAQVYPGHKGERGFPGPKGDVGPAGPPGSFPYDLSTFASILRREAGLKGEKGESGGGSHGQGIPGPPGNPGYPGLPGAKGDSVQGPPGPAGPPGPSGIGYEGRQGPPGPPGPPGLPGSSSYPGPHRQTISIPGPPGPPGPPGLPGTSGGLSGATVLQTYQTMINLAHRLQEGTIVLVQERSELYVRVREGFRKILLGEYTTSSGPGQDNEVAAEQAPVVPYPHRNKDSDYLQSTEENSNNPNVPSGARPWSDPRLPEPPRVNPPPPPDHHHHGNPRPLEPSPVHTHQEFNPVLHLVALNTPLSGSMKSIRGVDFQCFEQARKAGLHGTFRAFLSSRLQDLYSIVRRADRQSVQIVNLRDEVLFDNWESLFSGSEAQMRPGASIFSFDGKDVATDPTWPQKMVWHGSDSKGRRLTESYCETWRTDEGAVTGQASSLTSGKLLEQRPQSCNKNFIVLCIENSFMTNNRK

Target ORF information:

RefSeq Version	XM_018097368.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type XVIII, alpha 1 (col18a1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097368.1

ORF Insert Sequence:

ATGTATAGGG ACTTCTCTCT CCTCTTCCAT GTCCAGCCAA CCACAGACAA GGCCGGCATC 
CTCTTTGCCA TCACAGATGC CTCTCAGTCT ATTGTGTATA TCGGGGTGAA GCTTTCTGAA 
GTCAAGGATG GGAAGCAAGA CATCATCTTC TACTATACAG AGCCTGGCTC ACAGGATTCC 
TATGTAGCTG CCACCTTCAA TGTCCCATCA CTGGTGAATC GTTGGTCTCG CTTTGCAATC 
AGTGTGGAGG AAGAAGAAGT TGTGCTGTTT ATGGATTGCG ATGAGCTGGA AAGGGTGCGC 
TTCGAGAGAT CACCTGATGA GATGGAACTA GAAGATGGCT CTGGGCTCTT TGTTGCTCAA 
GCAGGTGGCG CTGATCCAGA CAGATATCAG GGTGTAATAG CAGATCTAAA GATCGTAGCC 
GATCCAAGAG CCGCAGAACA TCAGTGTGAG GAGGATGGAG ATGACTCTGA TGGGATCTCT 
GGAGATTTTG GCAGTGGCAT TGCGGACAAT GATTTGGTGA CTTCTCCTCC TGTGATAATC 
AAAGTGCAGC CTCCTATAAC TGTCCCTCCC ATACAGAGGA CCCCTGTGGA GAAAGAGATT 
GTTCAGACAG GTCAAGGCCA GCAAGGGGTA AAAGGAGAGA AGGGTGATCG TGGAGAGAAG 
GGTGAGAGGG GACTGACTGG CCCTAAGGGA GACGGTGGGA ATGCAAGTCC AACTTCTGAT 
TCCAAAGGAG AAAAGGGTCA GAAAGGAGAG GTCGGAGTGA AGGGAAGTGC AGGCTTTGGC 
TATCCCGGGC CTAAAGGGCA GAAAGGAGAT CCTGGAGACT TGGGACCAAG AGGACCACCA 
GGCCCACCTG GACCATCTAT ATTACAGAAA AGTGATGGAA CTACAGTGCA ACAGGTCACA 
GGACCTCCAG GTCCTCAAGG CCCTGCGGGA CCACCAGGAA AAGAAGGGGA ACCAGGTGAC 
CCTGGTGAAG ATGGAAAAAC TGGCGAGGTT GGTCCCCAAG GATTTCCAGG GACCCCAGGA 
GATTCTGGGC AGAAGGGAGA CAAGGGTGAT TCTGGTGTTG GTATCAGAGG TGCTCCTGGG 
CCACCAGGTC CGCCAGGCCC CCCTGGACCT TCTAGAACCG ACAAGCTGAC ATTCATTGAC 
ATGGAAGGTT CTGGATATGG TGGAGATTCG GAGCACATCA GGGGAGCCCC TGGTGTGCCT 
GGGCCCCCAG GTCCTCCAGG TGTACCAGGG TTACCCGGAG TACCCGGAAC TTTTGGCATG 
AACAACACAG GATTGCCAGG TCCACCTGGA TTGCCAGGTC TTCCCGGAAA GGAAGGAATT 
CCAGGTCCTC CTGGCCCCCC AGGACTCCCT GGAAAAGACG GGTTGTCTGG TACACCTGGT 
GCTGCTGGAT TACGGGGTGA AGATGGAGAG ATTGGTCTTC CAGGTGCTCC TGGAACAAAG 
GGTAGCACTG GTGAGCCAGG ACTACCTGGA CCTCCTGGAC AATCTGGTTT AGCTGGACTT 
CCTGGACCAA TGGGACCACG CGGGCTGCCA GGTCCTCCAG GACCACCTGG GCCTGTGGGT 
CATGGATCTG CAATTGGCTT TGATGACATG GAAGCCTCAG GGCTTGGATT TGGCTTGAGA 
GAAGGGACAC AGGGGGTGCC TGGACTTCCC GGGGCAAAGG GAGAGCCAGG ACATCCAGGG 
CTGCCAGGAG TGAAGGGAGA TAGTGGTTTT CCAGGCCTTG AAGGAAGGCC GGGGTTGGAT 
GGATTCCCAG GTCCAGAGGG ATTAAAGGGT GAGAAAGGTG AACGTGGTGA GCGGGGAGAA 
TCTGGCCGTG ATGGTGTTGG TATTCCAGGT CCACCGGGAC CTCCTGGTCA GGTGCTTTAT 
TTACCATCCG ATGACAAGAC TATTGTTGGT GCCATTCCTG GACCAGAGGG CAGGCAAGGT 
GTAGCTGGAC TTCCGGGCCC AAGGGGACCC AAAGGAGAGG CGGGCTTGAA CGGTATCCCA 
GGAACCTTTG GAGTAAAGGG AGAGAAGGGA GAGCCTGGAA TTATTATGGG ACCAGATAGC 
ACCATTTACA CTGATTCATC TAAAGGAGAG AAGGGTGACG AAGGACCAAG GGGACCAGCA 
GGAGCTGCGG GACCATTTGG ACAAAAGGGA GAAATCGGGT TCCCAGGACG CCCTGGACGT 
CCTGGTATGA ATGGACTGAA AGGGGAGAAA GGAGAACCTA GTGGTGCAAG TGGTGGCTTT 
GGACTGAGGG GGCCACCTGG TCCTCCTGGC CCACCCGGTC CTCCTGGCAG TGTGGTGTAT 
GGCGATCCAA ATAACCCAGG AGCCCAAGTA TATCCAGGTC ATAAAGGGGA GAGAGGATTT 
CCTGGACCCA AAGGTGATGT TGGTCCTGCT GGCCCACCAG GCTCTTTCCC ATATGATTTA 
AGTACCTTTG CCTCGATTTT AAGGAGAGAA GCTGGACTCA AAGGAGAGAA GGGCGAATCT 
GGGGGTGGTT CCCATGGACA GGGGATCCCA GGGCCACCTG GAAACCCTGG ATATCCAGGA 
CTGCCGGGAG CTAAAGGAGA CAGTGTTCAA GGTCCTCCAG GCCCAGCCGG CCCACCAGGA 
CCTTCTGGAA TCGGTTATGA AGGAAGGCAA GGTCCACCAG GGCCCCCAGG TCCTCCAGGC 
CTGCCAGGTT CCTCCTCTTA TCCTGGGCCT CACAGGCAAA CCATCAGCAT ACCTGGACCT 
CCTGGCCCAC CAGGCCCACC AGGGCTCCCA GGAACATCAG GAGGATTATC TGGGGCTACT 
GTGCTACAGA CCTATCAGAC TATGATCAAC TTAGCCCACA GGCTGCAGGA AGGGACAATT 
GTGCTGGTCC AGGAGAGGTC GGAACTGTAT GTGCGTGTGA GAGAAGGCTT CCGAAAAATT 
CTTCTTGGTG AATATACAAC ATCCTCTGGC CCTGGACAGG ATAATGAAGT TGCTGCGGAG 
CAGGCCCCTG TAGTTCCATA TCCACACCGT AACAAAGATT CTGATTACCT CCAGTCTACA 
GAGGAGAACA GCAACAATCC AAATGTGCCC TCAGGTGCCC GACCCTGGAG CGATCCTCGG 
TTACCAGAAC CACCAAGAGT AAACCCACCA CCACCCCCAG ACCATCACCA CCATGGAAAC 
CCTCGTCCAT TGGAACCATC CCCAGTGCAC ACACACCAAG AGTTTAATCC TGTGCTTCAC 
CTTGTGGCCC TGAACACTCC ACTGAGTGGC AGCATGAAAT CCATCCGCGG AGTGGACTTC 
CAGTGCTTTG AGCAAGCCCG TAAAGCTGGA CTACATGGCA CATTTAGAGC GTTTTTGTCT 
TCCCGACTTC AAGACCTGTA CAGCATTGTA CGCAGAGCTG ATCGACAATC TGTGCAAATT 
GTTAATCTTA GGGATGAAGT GTTATTTGAC AACTGGGAGT CCCTGTTCTC TGGTTCTGAG 
GCTCAGATGA GGCCTGGTGC GAGTATCTTC TCTTTTGATG GAAAAGATGT TGCAACAGAT 
CCTACATGGC CACAGAAGAT GGTTTGGCAT GGGTCTGACT CGAAGGGACG GCGGCTTACT 
GAAAGCTATT GCGAGACCTG GCGCACAGAT GAAGGTGCAG TAACTGGTCA GGCATCATCG 
CTGACGAGTG GGAAACTCTT AGAACAGCGC CCACAGAGTT GCAACAAGAA CTTCATTGTT 
TTGTGTATTG AGAACAGCTT CATGACCAAT AACCGGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	col18a1
Entrez Gene ID	394845
Full Name	collagen type XVIII alpha 1
Synonyms	kno,kno1
General protein information	Preferred Names collagen type XVIII alpha 1 Names collagen alpha-1(XVIII) chain
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Rattus norvegicus (Norway rat)	Col18a1	NP_445941.2
Pan troglodytes (chimpanzee)	COL18A1	XP_531500.4
Homo sapiens (human)	COL18A1	NP_085059.2
	COL18A1	XP_002803149.1
Mus musculus (house mouse)	Col18a1	NP_001103461.1
Xenopus tropicalis (tropical clawed frog)	col18a1	NP_989236.1
Gallus gallus (chicken)	COL18A1	NP_989495.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

col18a1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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