col2a1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	col2a1
Entrez Gene ID	394828
Full Name	collagen, type II, alpha 1
Synonyms	col11a3,col2a1-a,col2a1-b,xcol2a1
General protein information	Preferred Names collagen, type II, alpha 1 Names collagen alpha-1(II) chain X-col II alpha-1 type II collagen
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012956663.2	XP_012812117.1	collagen alpha-1(II) chain isoform X2
NM_203889.1	NP_989220.1	collagen alpha-1(II) chain precursor
XM_012956661.2	XP_012812115.1	collagen alpha-1(II) chain isoform X1
XM_012956661.3	XP_012812115.1	collagen alpha-1(II) chain isoform X1
XM_012956663.3	XP_012812117.1	collagen alpha-1(II) chain isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	COL2A1	NP_001001135.2
Gallus gallus (chicken)	COL2A1	NP_989757.1
Xenopus tropicalis (tropical clawed frog)	col2a1	NP_989220.1
	COL2A1	XP_001100739.1
Canis lupus familiaris (dog)	COL2A1	NP_001006952.1
Rattus norvegicus (Norway rat)	Col2a1	NP_037061.1
Danio rerio (zebrafish)	col2a1a	NP_571367.1
Pan troglodytes (chimpanzee)	COL2A1	XP_509026.2
Homo sapiens (human)	COL2A1	NP_001835.3
Mus musculus (house mouse)	Col2a1	NP_112440.2

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following col2a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col2a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa26091	XM_012956661.3 Latest version!	Xenopus tropicalis collagen, type II, alpha 1 (col2a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa51238	XM_012956663.3 Latest version!	Xenopus tropicalis collagen, type II, alpha 1 (col2a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa21643	NM_203889.1 Latest version!	Xenopus tropicalis collagen, type II, alpha 1 (col2a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OXa26092	XM_012956663.2 Latest version!	Xenopus tropicalis collagen, type II, alpha 1 (col2a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
View Old Accession Versions >>
OXa26091	XM_012956661.2	Xenopus tropicalis collagen, type II, alpha 1 (col2a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col2a1 ( XM_012956661.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812115.1 Xenopus tropicalis collagen, type II, alpha 1 (col2a1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa26091
Clone ID Related Accession (Same CDS sequence)	XM_012956661.2 , XM_012956661.3
Accession Version	XM_012956661.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(II) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956661.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGCT TTGTGGATTC AAGGACTCTA GTGCTGTTCG CAGCCACACA AGTCATTTTA 
TTAGCGGTTG TACGATGCCA AGATGAAGAA GATGTCCTGG CCACAGGCAG CTGCGTGCAG 
CATGGGCAGA GGTATAGTGA TAAAGATGTG TGGAAACCAG AGCCCTGCCA AATCTGTGTC 
TGTGACACTG GGAATGTTCT CTGCGATGAG ATAATCTGCG AAGATCCCAA AGATTGCCCC 
AATGCTGAGA TCCCCTTCGG AGAGTGCTGC CCCATCTGCC CAACTGAGCA ATCTTCTACC 
TCCAGTGGGC AAGGAGTACT CAAGGGCCAG AAAGGTGAAC CCGGTGATAT TAAAGATGTT 
GTAGGACCAA AAGGACCACC TGGACCACAG GGACCTTCTG GTGAACAAGG ACCTAGAGGA 
GATCGTGGAG ACAAGGGCGA GAAAGGTGCT CCTGGACCCC GTGGAAGAGA TGGCGAACCC 
GGTACTCCTG GGAACCCAGG ACCTGTTGGA CCCCCTGGAC CTCCTGGACC CCCTGGCCTT 
GGCGGAAACT TTGCTGCTCA AATGACAGGA GGATTTGATG AAAAAGCTGG TGGTGCACAA 
ATGGGAGTTA TGCAAGGACC AATGGGCCCT ATGGGACCAA GAGGACCTCC CGGCCCAACA 
GGAGCTCCTG GACCTCAAGG TTTCCAAGGC AATCCCGGTG AACCTGGTGA ACCTGGCGCT 
GGTGGTCCAA TGGGTCCCCG TGGTCCTCCT GGCCCTGCTG GAAAGCCTGG TGATGATGGT 
GAAGCTGGCA AACCTGGCAA ATCTGGTGAG CGTGGACCCC CAGGACCACA GGGAGCTAGA 
GGTTTCCCTG GAACCCCAGG ACTTCCAGGA GTAAAAGGAC ACAGGGGTTA CCCAGGTCTT 
GATGGGTCAA AGGGTGAAGC TGGTGCTGCT GGTGCCAAGG GAGAAGGTGG TGCTACTGGT 
GAAGCTGGAT CTCCTGGTCC AATGGGTCCC CGTGGTCTTC CAGGTGAGAG AGGACGCCCT 
GGAGCTTCTG GTGCTGCTGG TGCTCGTGGT AATGATGGTC TTCCTGGCCC TGCTGGTCCC 
CCAGGACCTG TTGGCCCTGC TGGTGCTCCT GGTTTCCCTG GTGCTCCTGG TTCAAAGGGT 
GAAGCTGGCC CAACTGGTGC TCGTGGACCT GAGGGTGCTC AAGGACCCAG AGGAGAATCT 
GGTACCCCTG GATCTCCTGG ACCTGCTGGA GCTTCTGGTA ACCCTGGTAC TGATGGTATT 
CCTGGCGCCA AAGGTTCATC TGGTGCTCCT GGTATTGCTG GTGCCCCTGG TTTCCCTGGA 
CCACGTGGTC CTCCAGGACC TCAGGGAGCT ACTGGTCCTC TTGGTCCCAA AGGCCAGACT 
GGTGACCCCG GTGTTGCAGG TTTCAAGGGT GAACATGGTC CCAAAGGTGA AATCGGGTCT 
GCAGGTCCTC AGGGTGCTCC TGGCCCAGCT GGTGAAGAAG GCAAGAGAGG AGCCCGTGGT 
GAACCTGGTG CTGCTGGACC CCTTGGTCCT CCTGGAGAGA GAGGTGCTCC TGGTAATCGT 
GGTTTCCCTG GTCAAGATGG TCTTGCTGGT CCTAAGGGTG CTCCTGGTGA ACGTGGTGTT 
CCAGGTCTTG GTGGACCAAA GGGTGCTAAT GGTGATCCTG GCCGTCCTGG CGAACCTGGT 
CTCCCTGGTG CTAGGGGTCT TACTGGCCGT CCTGGTGATG CTGGTCCTCA GGGAAAAGTT 
GGGCCCTCTG GTGCTTCTGG TGAAGATGGT CGTCCTGGAC CTCCTGGGCC ACAAGGTGCT 
CGTGGTCAGC CTGGTGTTAT GGGTTTCCCT GGGCCTAAAG GTGCCAATGG CGAACCTGGC 
AAAGCTGGTG AGAAAGGACT GCTTGGTGCT CCTGGTCTGA GGGGTCTGCC TGGAAAAGAT 
GGTGAAACTG GTGCTCAAGG TCCCAATGGT CCAGCTGGAC CTGCTGGTGA AAGAGGTGAA 
CAAGGACCTC CTGGCCCATC TGGCTTCCAG GGACTTCCTG GACCTCCCGG TTCTCCCGGA 
GAAGGTGGCA AACCAGGTGA TCAGGGTGTT CCCGGTGAAG CAGGTGCCCC TGGTCTTGTT 
GGCCCAAGAG GTGAGCGTGG TTTCCCAGGT GAACGTGGTT CTTCAGGTCC TCAAGGTCTT 
CAGGGTCCTC GTGGTCTTCC TGGAACTCCT GGTACTGATG GTCCCAAGGG TGCAACTGGT 
CCATCTGGTC CCAATGGTGC CCAAGGTCCT CCTGGTCTTC AAGGTATGCC TGGTGAGAGA 
GGAGCTGCTG GTATTTCTGG ACCCAAGGGT GATAGAGGTG ATACTGGTGA GAAAGGCCCA 
GAGGGTGCTC CTGGTAAAGA TGGTTCAAGA GGTTTGACTG GTCCAATTGG TCCCCCTGGT 
CCATCTGGTC CTAATGGTGA GAAGGGAGAA TCTGGTCCTT CCGGTCCAGC TGGTATTGTT 
GGTGCCCGTG GTGCCCCTGG TGATCGTGGT GAGACTGGCC CTCCAGGTCC TGCTGGCTTT 
GCTGGTCCTC CTGGTGCTAC TGGATTCCCT GGTGCTGCTG GAAGAGTTGG TCCCCCTGGT 
CCCAATGGTA ACCCTGGACC TTCAGGTGCT CCTGGATCTG CTGGTAAAGA AGGGCCAAAG 
GGTGCACGTG GTGATGCTGG TCCTACTGGT CGTGCTGGTG ATCCAGGTCT TCAAGGTCCT 
GCTGGTGTTC CTGGAGAGAA GGGAGAGTCT GGTGAAGATG GTCCTTCTGG TCCAGATGGT 
CCCCCAGGTC CTCAAGGTTT GTCTGGACAG CGTGGTATTG TTGGTCTGCC AGGTCAGCGT 
GGTGAAAGAG GATTCCCTGG ACTTCCTGGT CCATCTGGTG AACCTGGAAA ACAAGGAGGC 
CCTGGATCTG CTGGAGACCG TGGACCCCCT GGCCCTGTTG GCCCACCTGG TTTGACTGGA 
CCTGCTGGAG AGCCTGGACG TGAAGGTAAC GCTGGATCTG ATGGCCCACC TGGTCGTGAT 
GGTGCTACTG GAATTAAGGG TGATCGTGGT GAGACTGGTC CTCTTGGTGC TCCTGGTGCT 
CCCGGTGCCC CTGGTGCTCC TGGACCTGTT GGCCCAACTG GAAAACAAGG AGACAGAGGA 
GAATCTGGAC CCCAAGGTCC ACTTGGTCCA TCTGGTCCTG CTGGTGCTCG TGGCTTGCCT 
GGTCCTCAAG GTCCACGTGG TGATAAGGGT GAAGCTGGTG AGGCAGGAGA GAGAGGACAG 
AAAGGTCACA GAGGATTCAC TGGTCTTCAG GGTCTGCCAG GTCCCCCAGG TACTGCTGGT 
GACCAAGGTG CTTCTGGTCC TGCCGGTCCT GGTGGTCCTA GAGGTCCCCC TGGCCCTGTT 
GGTCCTTCTG GCAAAGATGG CTCTAATGGT CTTCCTGGTC CAATTGGTCC ACCTGGTCCA 
CGCGGTCGCG GTGGTGAAAC TGGTCCTGCT GGTCCACCTG GTCAACCTGG CCCACCTGGT 
CCCCCTGGTC CTCCTGGCCC TGGAATTGAC ATGTCTGCTT TCGCCGGCCT ATCTCAACCT 
GAAAAAGGAC CCGACCCAAT GCGTTACATG CGTGCTGACC AAGCCTCCAG CTCTGTCCCT 
CAGCGTGATG TTGATGTTGA AGCCACTTTG AAATCCCTTA ATAACCAGAT CGAAAGCATC 
CGCAGCCCAG ATGGTACCAA GAAGAACCCA GCCCGCACAT GCCGTGACCT GAAACTCTGC 
CATCCTGAAT GGAAGAGCGG TGACTATTGG ATCGATCCCA ACCAGGGCTG CACTGTAGAT 
GCTATCAAAG TCTTCTGCAA CATGGAGACT GGAGAGACCT GTGTATACCC CAACCCATCA 
AAGATCCCCA AGAAGAACTG GTGGTCCGCC AAGGGCAAGG AAAAGAAACA CATTTGGTTT 
GGAGAGACAA TCAATGGTGG CTTCCAATTT AGCTATGGAG ATGACAGCTC AGCACCCAAC 
ACTGCCAACA TTCAGATGAC CTTCCTGCGT CTGCTCTCCA CTGATGCAAC CCAGAACATC 
ACCTATCACT GCAAGAACAG CATTGCATTT ATGGATGAAG CTTCAGGCAA CCTTAAGAAG 
GCTGTTCTAC TCCAAGGATC CAATGATGTA GAAATCAGAG CTGAGGGCAA CAGTAGATTC 
ACATACAATG CCTTGGAAGA TGGCTGCAAG AAACACACTG GCAAATGGAG CAAGACAGTA 
ATTGAATACA GGACACAGAA AACATCCCGC CTGCCCATCG TAGACATTGC ACCTATGGAT 
ATTGGTGGCG CTGATCAGGA ATTTGGTGTT GACATTGGCC CAGTCTGCTT CTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812115.1
CDS	199..4515
Translation	MFSFVDSRTLVLFAATQVILLAVVRCQDEEDVLATGSCVQHGQRYSDKDVWKPEPCQICVCDTGNVLCDEIICEDPKDCPNAEIPFGECCPICPTEQSSTSSGQGVLKGQKGEPGDIKDVVGPKGPPGPQGPSGEQGPRGDRGDKGEKGAPGPRGRDGEPGTPGNPGPVGPPGPPGPPGLGGNFAAQMTGGFDEKAGGAQMGVMQGPMGPMGPRGPPGPTGAPGPQGFQGNPGEPGEPGAGGPMGPRGPPGPAGKPGDDGEAGKPGKSGERGPPGPQGARGFPGTPGLPGVKGHRGYPGLDGSKGEAGAAGAKGEGGATGEAGSPGPMGPRGLPGERGRPGASGAAGARGNDGLPGPAGPPGPVGPAGAPGFPGAPGSKGEAGPTGARGPEGAQGPRGESGTPGSPGPAGASGNPGTDGIPGAKGSSGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQTGDPGVAGFKGEHGPKGEIGSAGPQGAPGPAGEEGKRGARGEPGAAGPLGPPGERGAPGNRGFPGQDGLAGPKGAPGERGVPGLGGPKGANGDPGRPGEPGLPGARGLTGRPGDAGPQGKVGPSGASGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLLGAPGLRGLPGKDGETGAQGPNGPAGPAGERGEQGPPGPSGFQGLPGPPGSPGEGGKPGDQGVPGEAGAPGLVGPRGERGFPGERGSSGPQGLQGPRGLPGTPGTDGPKGATGPSGPNGAQGPPGLQGMPGERGAAGISGPKGDRGDTGEKGPEGAPGKDGSRGLTGPIGPPGPSGPNGEKGESGPSGPAGIVGARGAPGDRGETGPPGPAGFAGPPGATGFPGAAGRVGPPGPNGNPGPSGAPGSAGKEGPKGARGDAGPTGRAGDPGLQGPAGVPGEKGESGEDGPSGPDGPPGPQGLSGQRGIVGLPGQRGERGFPGLPGPSGEPGKQGGPGSAGDRGPPGPVGPPGLTGPAGEPGREGNAGSDGPPGRDGATGIKGDRGETGPLGAPGAPGAPGAPGPVGPTGKQGDRGESGPQGPLGPSGPAGARGLPGPQGPRGDKGEAGEAGERGQKGHRGFTGLQGLPGPPGTAGDQGASGPAGPGGPRGPPGPVGPSGKDGSNGLPGPIGPPGPRGRGGETGPAGPPGQPGPPGPPGPPGPGIDMSAFAGLSQPEKGPDPMRYMRADQASSSVPQRDVDVEATLKSLNNQIESIRSPDGTKKNPARTCRDLKLCHPEWKSGDYWIDPNQGCTVDAIKVFCNMETGETCVYPNPSKIPKKNWWSAKGKEKKHIWFGETINGGFQFSYGDDSSAPNTANIQMTFLRLLSTDATQNITYHCKNSIAFMDEASGNLKKAVLLQGSNDVEIRAEGNSRFTYNALEDGCKKHTGKWSKTVIEYRTQKTSRLPIVDIAPMDIGGADQEFGVDIGPVCFL

Target ORF information:

RefSeq Version	XM_012956661.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type II, alpha 1 (col2a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956661.3

ORF Insert Sequence:

ATGTTTAGCT TTGTGGATTC AAGGACTCTA GTGCTGTTCG CAGCCACACA AGTCATTTTA 
TTAGCGGTTG TACGATGCCA AGATGAAGAA GATGTCCTGG CCACAGGCAG CTGCGTGCAG 
CATGGGCAGA GGTATAGTGA TAAAGATGTG TGGAAACCAG AGCCCTGCCA AATCTGTGTC 
TGTGACACTG GGAATGTTCT CTGCGATGAG ATAATCTGCG AAGATCCCAA AGATTGCCCC 
AATGCTGAGA TCCCCTTCGG AGAGTGCTGC CCCATCTGCC CAACTGAGCA ATCTTCTACC 
TCCAGTGGGC AAGGAGTACT CAAGGGCCAG AAAGGTGAAC CCGGTGATAT TAAAGATGTT 
GTAGGACCAA AAGGACCACC TGGACCACAG GGACCTTCTG GTGAACAAGG ACCTAGAGGA 
GATCGTGGAG ACAAGGGCGA GAAAGGTGCT CCTGGACCCC GTGGAAGAGA TGGCGAACCC 
GGTACTCCTG GGAACCCAGG ACCTGTTGGA CCCCCTGGAC CTCCTGGACC CCCTGGCCTT 
GGCGGAAACT TTGCTGCTCA AATGACAGGA GGATTTGATG AAAAAGCTGG TGGTGCACAA 
ATGGGAGTTA TGCAAGGACC AATGGGCCCT ATGGGACCAA GAGGACCTCC CGGCCCAACA 
GGAGCTCCTG GACCTCAAGG TTTCCAAGGC AATCCCGGTG AACCTGGTGA ACCTGGCGCT 
GGTGGTCCAA TGGGTCCCCG TGGTCCTCCT GGCCCTGCTG GAAAGCCTGG TGATGATGGT 
GAAGCTGGCA AACCTGGCAA ATCTGGTGAG CGTGGACCCC CAGGACCACA GGGAGCTAGA 
GGTTTCCCTG GAACCCCAGG ACTTCCAGGA GTAAAAGGAC ACAGGGGTTA CCCAGGTCTT 
GATGGGTCAA AGGGTGAAGC TGGTGCTGCT GGTGCCAAGG GAGAAGGTGG TGCTACTGGT 
GAAGCTGGAT CTCCTGGTCC AATGGGTCCC CGTGGTCTTC CAGGTGAGAG AGGACGCCCT 
GGAGCTTCTG GTGCTGCTGG TGCTCGTGGT AATGATGGTC TTCCTGGCCC TGCTGGTCCC 
CCAGGACCTG TTGGCCCTGC TGGTGCTCCT GGTTTCCCTG GTGCTCCTGG TTCAAAGGGT 
GAAGCTGGCC CAACTGGTGC TCGTGGACCT GAGGGTGCTC AAGGACCCAG AGGAGAATCT 
GGTACCCCTG GATCTCCTGG ACCTGCTGGA GCTTCTGGTA ACCCTGGTAC TGATGGTATT 
CCTGGCGCCA AAGGTTCATC TGGTGCTCCT GGTATTGCTG GTGCCCCTGG TTTCCCTGGA 
CCACGTGGTC CTCCAGGACC TCAGGGAGCT ACTGGTCCTC TTGGTCCCAA AGGCCAGACT 
GGTGACCCCG GTGTTGCAGG TTTCAAGGGT GAACATGGTC CCAAAGGTGA AATCGGGTCT 
GCAGGTCCTC AGGGTGCTCC TGGCCCAGCT GGTGAAGAAG GCAAGAGAGG AGCCCGTGGT 
GAACCTGGTG CTGCTGGACC CCTTGGTCCT CCTGGAGAGA GAGGTGCTCC TGGTAATCGT 
GGTTTCCCTG GTCAAGATGG TCTTGCTGGT CCTAAGGGTG CTCCTGGTGA ACGTGGTGTT 
CCAGGTCTTG GTGGACCAAA GGGTGCTAAT GGTGATCCTG GCCGTCCTGG CGAACCTGGT 
CTCCCTGGTG CTAGGGGTCT TACTGGCCGT CCTGGTGATG CTGGTCCTCA GGGAAAAGTT 
GGGCCCTCTG GTGCTTCTGG TGAAGATGGT CGTCCTGGAC CTCCTGGGCC ACAAGGTGCT 
CGTGGTCAGC CTGGTGTTAT GGGTTTCCCT GGGCCTAAAG GTGCCAATGG CGAACCTGGC 
AAAGCTGGTG AGAAAGGACT GCTTGGTGCT CCTGGTCTGA GGGGTCTGCC TGGAAAAGAT 
GGTGAAACTG GTGCTCAAGG TCCCAATGGT CCAGCTGGAC CTGCTGGTGA AAGAGGTGAA 
CAAGGACCTC CTGGCCCATC TGGCTTCCAG GGACTTCCTG GACCTCCCGG TTCTCCCGGA 
GAAGGTGGCA AACCAGGTGA TCAGGGTGTT CCCGGTGAAG CAGGTGCCCC TGGTCTTGTT 
GGCCCAAGAG GTGAGCGTGG TTTCCCAGGT GAACGTGGTT CTTCAGGTCC TCAAGGTCTT 
CAGGGTCCTC GTGGTCTTCC TGGAACTCCT GGTACTGATG GTCCCAAGGG TGCAACTGGT 
CCATCTGGTC CCAATGGTGC CCAAGGTCCT CCTGGTCTTC AAGGTATGCC TGGTGAGAGA 
GGAGCTGCTG GTATTTCTGG ACCCAAGGGT GATAGAGGTG ATACTGGTGA GAAAGGCCCA 
GAGGGTGCTC CTGGTAAAGA TGGTTCAAGA GGTTTGACTG GTCCAATTGG TCCCCCTGGT 
CCATCTGGTC CTAATGGTGA GAAGGGAGAA TCTGGTCCTT CCGGTCCAGC TGGTATTGTT 
GGTGCCCGTG GTGCCCCTGG TGATCGTGGT GAGACTGGCC CTCCAGGTCC TGCTGGCTTT 
GCTGGTCCTC CTGGTGCTAC TGGATTCCCT GGTGCTGCTG GAAGAGTTGG TCCCCCTGGT 
CCCAATGGTA ACCCTGGACC TTCAGGTGCT CCTGGATCTG CTGGTAAAGA AGGGCCAAAG 
GGTGCACGTG GTGATGCTGG TCCTACTGGT CGTGCTGGTG ATCCAGGTCT TCAAGGTCCT 
GCTGGTGTTC CTGGAGAGAA GGGAGAGTCT GGTGAAGATG GTCCTTCTGG TCCAGATGGT 
CCCCCAGGTC CTCAAGGTTT GTCTGGACAG CGTGGTATTG TTGGTCTGCC AGGTCAGCGT 
GGTGAAAGAG GATTCCCTGG ACTTCCTGGT CCATCTGGTG AACCTGGAAA ACAAGGAGGC 
CCTGGATCTG CTGGAGACCG TGGACCCCCT GGCCCTGTTG GCCCACCTGG TTTGACTGGA 
CCTGCTGGAG AGCCTGGACG TGAAGGTAAC GCTGGATCTG ATGGCCCACC TGGTCGTGAT 
GGTGCTACTG GAATTAAGGG TGATCGTGGT GAGACTGGTC CTCTTGGTGC TCCTGGTGCT 
CCCGGTGCCC CTGGTGCTCC TGGACCTGTT GGCCCAACTG GAAAACAAGG AGACAGAGGA 
GAATCTGGAC CCCAAGGTCC ACTTGGTCCA TCTGGTCCTG CTGGTGCTCG TGGCTTGCCT 
GGTCCTCAAG GTCCACGTGG TGATAAGGGT GAAGCTGGTG AGGCAGGAGA GAGAGGACAG 
AAAGGTCACA GAGGATTCAC TGGTCTTCAG GGTCTGCCAG GTCCCCCAGG TACTGCTGGT 
GACCAAGGTG CTTCTGGTCC TGCCGGTCCT GGTGGTCCTA GAGGTCCCCC TGGCCCTGTT 
GGTCCTTCTG GCAAAGATGG CTCTAATGGT CTTCCTGGTC CAATTGGTCC ACCTGGTCCA 
CGCGGTCGCG GTGGTGAAAC TGGTCCTGCT GGTCCACCTG GTCAACCTGG CCCACCTGGT 
CCCCCTGGTC CTCCTGGCCC TGGAATTGAC ATGTCTGCTT TCGCCGGCCT ATCTCAACCT 
GAAAAAGGAC CCGACCCAAT GCGTTACATG CGTGCTGACC AAGCCTCCAG CTCTGTCCCT 
CAGCGTGATG TTGATGTTGA AGCCACTTTG AAATCCCTTA ATAACCAGAT CGAAAGCATC 
CGCAGCCCAG ATGGTACCAA GAAGAACCCA GCCCGCACAT GCCGTGACCT GAAACTCTGC 
CATCCTGAAT GGAAGAGCGG TGACTATTGG ATCGATCCCA ACCAGGGCTG CACTGTAGAT 
GCTATCAAAG TCTTCTGCAA CATGGAGACT GGAGAGACCT GTGTATACCC CAACCCATCA 
AAGATCCCCA AGAAGAACTG GTGGTCCGCC AAGGGCAAGG AAAAGAAACA CATTTGGTTT 
GGAGAGACAA TCAATGGTGG CTTCCAATTT AGCTATGGAG ATGACAGCTC AGCACCCAAC 
ACTGCCAACA TTCAGATGAC CTTCCTGCGT CTGCTCTCCA CTGATGCAAC CCAGAACATC 
ACCTATCACT GCAAGAACAG CATTGCATTT ATGGATGAAG CTTCAGGCAA CCTTAAGAAG 
GCTGTTCTAC TCCAAGGATC CAATGATGTA GAAATCAGAG CTGAGGGCAA CAGTAGATTC 
ACATACAATG CCTTGGAAGA TGGCTGCAAG AAACACACTG GCAAATGGAG CAAGACAGTA 
ATTGAATACA GGACACAGAA AACATCCCGC CTGCCCATCG TAGACATTGC ACCTATGGAT 
ATTGGTGGCG CTGATCAGGA ATTTGGTGTT GACATTGGCC CAGTCTGCTT CTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col2a1 ( XM_012956663.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812117.1 Xenopus tropicalis collagen, type II, alpha 1 (col2a1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa51238
Clone ID Related Accession (Same CDS sequence)	XM_012956663.3
Accession Version	XM_012956663.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(II) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956663.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGCT TTGTGGATTC AAGGACTCTA GTGCTGTTCG CAGCCACACA AGTCATTTTA 
TTAGCGGTTG TACGATGCCA AGATGAAGAA GATGTCCGGC AAGGAGTACT CAAGGGCCAG 
AAAGGTGAAC CCGGTGATAT TAAAGATGTT GTAGGACCAA AAGGACCACC TGGACCACAG 
GGACCTTCTG GTGAACAAGG ACCTAGAGGA GATCGTGGAG ACAAGGGCGA GAAAGGTGCT 
CCTGGACCCC GTGGAAGAGA TGGCGAACCC GGTACTCCTG GGAACCCAGG ACCTGTTGGA 
CCCCCTGGAC CTCCTGGACC CCCTGGCCTT GGCGGAAACT TTGCTGCTCA AATGACAGGA 
GGATTTGATG AAAAAGCTGG TGGTGCACAA ATGGGAGTTA TGCAAGGACC AATGGGCCCT 
ATGGGACCAA GAGGACCTCC CGGCCCAACA GGAGCTCCTG GACCTCAAGG TTTCCAAGGC 
AATCCCGGTG AACCTGGTGA ACCTGGCGCT GGTGGTCCAA TGGGTCCCCG TGGTCCTCCT 
GGCCCTGCTG GAAAGCCTGG TGATGATGGT GAAGCTGGCA AACCTGGCAA ATCTGGTGAG 
CGTGGACCCC CAGGACCACA GGGAGCTAGA GGTTTCCCTG GAACCCCAGG ACTTCCAGGA 
GTAAAAGGAC ACAGGGGTTA CCCAGGTCTT GATGGGTCAA AGGGTGAAGC TGGTGCTGCT 
GGTGCCAAGG GAGAAGGTGG TGCTACTGGT GAAGCTGGAT CTCCTGGTCC AATGGGTCCC 
CGTGGTCTTC CAGGTGAGAG AGGACGCCCT GGAGCTTCTG GTGCTGCTGG TGCTCGTGGT 
AATGATGGTC TTCCTGGCCC TGCTGGTCCC CCAGGACCTG TTGGCCCTGC TGGTGCTCCT 
GGTTTCCCTG GTGCTCCTGG TTCAAAGGGT GAAGCTGGCC CAACTGGTGC TCGTGGACCT 
GAGGGTGCTC AAGGACCCAG AGGAGAATCT GGTACCCCTG GATCTCCTGG ACCTGCTGGA 
GCTTCTGGTA ACCCTGGTAC TGATGGTATT CCTGGCGCCA AAGGTTCATC TGGTGCTCCT 
GGTATTGCTG GTGCCCCTGG TTTCCCTGGA CCACGTGGTC CTCCAGGACC TCAGGGAGCT 
ACTGGTCCTC TTGGTCCCAA AGGCCAGACT GGTGACCCCG GTGTTGCAGG TTTCAAGGGT 
GAACATGGTC CCAAAGGTGA AATCGGGTCT GCAGGTCCTC AGGGTGCTCC TGGCCCAGCT 
GGTGAAGAAG GCAAGAGAGG AGCCCGTGGT GAACCTGGTG CTGCTGGACC CCTTGGTCCT 
CCTGGAGAGA GAGGTGCTCC TGGTAATCGT GGTTTCCCTG GTCAAGATGG TCTTGCTGGT 
CCTAAGGGTG CTCCTGGTGA ACGTGGTGTT CCAGGTCTTG GTGGACCAAA GGGTGCTAAT 
GGTGATCCTG GCCGTCCTGG CGAACCTGGT CTCCCTGGTG CTAGGGGTCT TACTGGCCGT 
CCTGGTGATG CTGGTCCTCA GGGAAAAGTT GGGCCCTCTG GTGCTTCTGG TGAAGATGGT 
CGTCCTGGAC CTCCTGGGCC ACAAGGTGCT CGTGGTCAGC CTGGTGTTAT GGGTTTCCCT 
GGGCCTAAAG GTGCCAATGG CGAACCTGGC AAAGCTGGTG AGAAAGGACT GCTTGGTGCT 
CCTGGTCTGA GGGGTCTGCC TGGAAAAGAT GGTGAAACTG GTGCTCAAGG TCCCAATGGT 
CCAGCTGGAC CTGCTGGTGA AAGAGGTGAA CAAGGACCTC CTGGCCCATC TGGCTTCCAG 
GGACTTCCTG GACCTCCCGG TTCTCCCGGA GAAGGTGGCA AACCAGGTGA TCAGGGTGTT 
CCCGGTGAAG CAGGTGCCCC TGGTCTTGTT GGCCCAAGAG GTGAGCGTGG TTTCCCAGGT 
GAACGTGGTT CTTCAGGTCC TCAAGGTCTT CAGGGTCCTC GTGGTCTTCC TGGAACTCCT 
GGTACTGATG GTCCCAAGGG TGCAACTGGT CCATCTGGTC CCAATGGTGC CCAAGGTCCT 
CCTGGTCTTC AAGGTATGCC TGGTGAGAGA GGAGCTGCTG GTATTTCTGG ACCCAAGGGT 
GATAGAGGTG ATACTGGTGA GAAAGGCCCA GAGGGTGCTC CTGGTAAAGA TGGTTCAAGA 
GGTTTGACTG GTCCAATTGG TCCCCCTGGT CCATCTGGTC CTAATGGTGA GAAGGGAGAA 
TCTGGTCCTT CCGGTCCAGC TGGTATTGTT GGTGCCCGTG GTGCCCCTGG TGATCGTGGT 
GAGACTGGCC CTCCAGGTCC TGCTGGCTTT GCTGGTCCTC CTGGTGCTGA TGGTCAAGCT 
GGTCTTAAGG GTGATCAAGG TGAATCTGGC CAGAAGGGTG ATGCTGGTGC TCCTGGTCCC 
CAGGGTCCAT CTGGTGCTCC TGGCCCACAG GGCCCAACTG GTGTTAATGG TCCTAAGGGA 
GCTCGTGGTG CTCAAGGTCC TCCTGGTGCT ACTGGATTCC CTGGTGCTGC TGGAAGAGTT 
GGTCCCCCTG GTCCCAATGG TAACCCTGGA CCTTCAGGTG CTCCTGGATC TGCTGGTAAA 
GAAGGGCCAA AGGGTGCACG TGGTGATGCT GGTCCTACTG GTCGTGCTGG TGATCCAGGT 
CTTCAAGGTC CTGCTGGTGT TCCTGGAGAG AAGGGAGAGT CTGGTGAAGA TGGTCCTTCT 
GGTCCAGATG GTCCCCCAGG TCCTCAAGGT TTGTCTGGAC AGCGTGGTAT TGTTGGTCTG 
CCAGGTCAGC GTGGTGAAAG AGGATTCCCT GGACTTCCTG GTCCATCTGG TGAACCTGGA 
AAACAAGGAG GCCCTGGATC TGCTGGAGAC CGTGGACCCC CTGGCCCTGT TGGCCCACCT 
GGTTTGACTG GACCTGCTGG AGAGCCTGGA CGTGAAGGTA ACGCTGGATC TGATGGCCCA 
CCTGGTCGTG ATGGTGCTAC TGGAATTAAG GGTGATCGTG GTGAGACTGG TCCTCTTGGT 
GCTCCTGGTG CTCCCGGTGC CCCTGGTGCT CCTGGACCTG TTGGCCCAAC TGGAAAACAA 
GGAGACAGAG GAGAATCTGG ACCCCAAGGT CCACTTGGTC CATCTGGTCC TGCTGGTGCT 
CGTGGCTTGC CTGGTCCTCA AGGTCCACGT GGTGATAAGG GTGAAGCTGG TGAGGCAGGA 
GAGAGAGGAC AGAAAGGTCA CAGAGGATTC ACTGGTCTTC AGGGTCTGCC AGGTCCCCCA 
GGTACTGCTG GTGACCAAGG TGCTTCTGGT CCTGCCGGTC CTGGTGGTCC TAGAGGTCCC 
CCTGGCCCTG TTGGTCCTTC TGGCAAAGAT GGCTCTAATG GTCTTCCTGG TCCAATTGGT 
CCACCTGGTC CACGCGGTCG CGGTGGTGAA ACTGGTCCTG CTGGTCCACC TGGTCAACCT 
GGCCCACCTG GTCCCCCTGG TCCTCCTGGC CCTGGAATTG ACATGTCTGC TTTCGCCGGC 
CTATCTCAAC CTGAAAAAGG ACCCGACCCA ATGCGTTACA TGCGTGCTGA CCAAGCCTCC 
AGCTCTGTCC CTCAGCGTGA TGTTGATGTT GAAGCCACTT TGAAATCCCT TAATAACCAG 
ATCGAAAGCA TCCGCAGCCC AGATGGTACC AAGAAGAACC CAGCCCGCAC ATGCCGTGAC 
CTGAAACTCT GCCATCCTGA ATGGAAGAGC GGTGACTATT GGATCGATCC CAACCAGGGC 
TGCACTGTAG ATGCTATCAA AGTCTTCTGC AACATGGAGA CTGGAGAGAC CTGTGTATAC 
CCCAACCCAT CAAAGATCCC CAAGAAGAAC TGGTGGTCCG CCAAGGGCAA GGAAAAGAAA 
CACATTTGGT TTGGAGAGAC AATCAATGGT GGCTTCCAAT TTAGCTATGG AGATGACAGC 
TCAGCACCCA ACACTGCCAA CATTCAGATG ACCTTCCTGC GTCTGCTCTC CACTGATGCA 
ACCCAGAACA TCACCTATCA CTGCAAGAAC AGCATTGCAT TTATGGATGA AGCTTCAGGC 
AACCTTAAGA AGGCTGTTCT ACTCCAAGGA TCCAATGATG TAGAAATCAG AGCTGAGGGC 
AACAGTAGAT TCACATACAA TGCCTTGGAA GATGGCTGCA AGAAACACAC TGGCAAATGG 
AGCAAGACAG TAATTGAATA CAGGACACAG AAAACATCCC GCCTGCCCAT CGTAGACATT 
GCACCTATGG ATATTGGTGG CGCTGATCAG GAATTTGGTG TTGACATTGG CCCAGTCTGC 
TTCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812117.1
CDS	199..4467
Translation	MFSFVDSRTLVLFAATQVILLAVVRCQDEEDVRQGVLKGQKGEPGDIKDVVGPKGPPGPQGPSGEQGPRGDRGDKGEKGAPGPRGRDGEPGTPGNPGPVGPPGPPGPPGLGGNFAAQMTGGFDEKAGGAQMGVMQGPMGPMGPRGPPGPTGAPGPQGFQGNPGEPGEPGAGGPMGPRGPPGPAGKPGDDGEAGKPGKSGERGPPGPQGARGFPGTPGLPGVKGHRGYPGLDGSKGEAGAAGAKGEGGATGEAGSPGPMGPRGLPGERGRPGASGAAGARGNDGLPGPAGPPGPVGPAGAPGFPGAPGSKGEAGPTGARGPEGAQGPRGESGTPGSPGPAGASGNPGTDGIPGAKGSSGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQTGDPGVAGFKGEHGPKGEIGSAGPQGAPGPAGEEGKRGARGEPGAAGPLGPPGERGAPGNRGFPGQDGLAGPKGAPGERGVPGLGGPKGANGDPGRPGEPGLPGARGLTGRPGDAGPQGKVGPSGASGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLLGAPGLRGLPGKDGETGAQGPNGPAGPAGERGEQGPPGPSGFQGLPGPPGSPGEGGKPGDQGVPGEAGAPGLVGPRGERGFPGERGSSGPQGLQGPRGLPGTPGTDGPKGATGPSGPNGAQGPPGLQGMPGERGAAGISGPKGDRGDTGEKGPEGAPGKDGSRGLTGPIGPPGPSGPNGEKGESGPSGPAGIVGARGAPGDRGETGPPGPAGFAGPPGADGQAGLKGDQGESGQKGDAGAPGPQGPSGAPGPQGPTGVNGPKGARGAQGPPGATGFPGAAGRVGPPGPNGNPGPSGAPGSAGKEGPKGARGDAGPTGRAGDPGLQGPAGVPGEKGESGEDGPSGPDGPPGPQGLSGQRGIVGLPGQRGERGFPGLPGPSGEPGKQGGPGSAGDRGPPGPVGPPGLTGPAGEPGREGNAGSDGPPGRDGATGIKGDRGETGPLGAPGAPGAPGAPGPVGPTGKQGDRGESGPQGPLGPSGPAGARGLPGPQGPRGDKGEAGEAGERGQKGHRGFTGLQGLPGPPGTAGDQGASGPAGPGGPRGPPGPVGPSGKDGSNGLPGPIGPPGPRGRGGETGPAGPPGQPGPPGPPGPPGPGIDMSAFAGLSQPEKGPDPMRYMRADQASSSVPQRDVDVEATLKSLNNQIESIRSPDGTKKNPARTCRDLKLCHPEWKSGDYWIDPNQGCTVDAIKVFCNMETGETCVYPNPSKIPKKNWWSAKGKEKKHIWFGETINGGFQFSYGDDSSAPNTANIQMTFLRLLSTDATQNITYHCKNSIAFMDEASGNLKKAVLLQGSNDVEIRAEGNSRFTYNALEDGCKKHTGKWSKTVIEYRTQKTSRLPIVDIAPMDIGGADQEFGVDIGPVCFL

Target ORF information:

RefSeq Version	XM_012956663.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type II, alpha 1 (col2a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956663.3

ORF Insert Sequence:

ATGTTTAGCT TTGTGGATTC AAGGACTCTA GTGCTGTTCG CAGCCACACA AGTCATTTTA 
TTAGCGGTTG TACGATGCCA AGATGAAGAA GATGTCCGGC AAGGAGTACT CAAGGGCCAG 
AAAGGTGAAC CCGGTGATAT TAAAGATGTT GTAGGACCAA AAGGACCACC TGGACCACAG 
GGACCTTCTG GTGAACAAGG ACCTAGAGGA GATCGTGGAG ACAAGGGCGA GAAAGGTGCT 
CCTGGACCCC GTGGAAGAGA TGGCGAACCC GGTACTCCTG GGAACCCAGG ACCTGTTGGA 
CCCCCTGGAC CTCCTGGACC CCCTGGCCTT GGCGGAAACT TTGCTGCTCA AATGACAGGA 
GGATTTGATG AAAAAGCTGG TGGTGCACAA ATGGGAGTTA TGCAAGGACC AATGGGCCCT 
ATGGGACCAA GAGGACCTCC CGGCCCAACA GGAGCTCCTG GACCTCAAGG TTTCCAAGGC 
AATCCCGGTG AACCTGGTGA ACCTGGCGCT GGTGGTCCAA TGGGTCCCCG TGGTCCTCCT 
GGCCCTGCTG GAAAGCCTGG TGATGATGGT GAAGCTGGCA AACCTGGCAA ATCTGGTGAG 
CGTGGACCCC CAGGACCACA GGGAGCTAGA GGTTTCCCTG GAACCCCAGG ACTTCCAGGA 
GTAAAAGGAC ACAGGGGTTA CCCAGGTCTT GATGGGTCAA AGGGTGAAGC TGGTGCTGCT 
GGTGCCAAGG GAGAAGGTGG TGCTACTGGT GAAGCTGGAT CTCCTGGTCC AATGGGTCCC 
CGTGGTCTTC CAGGTGAGAG AGGACGCCCT GGAGCTTCTG GTGCTGCTGG TGCTCGTGGT 
AATGATGGTC TTCCTGGCCC TGCTGGTCCC CCAGGACCTG TTGGCCCTGC TGGTGCTCCT 
GGTTTCCCTG GTGCTCCTGG TTCAAAGGGT GAAGCTGGCC CAACTGGTGC TCGTGGACCT 
GAGGGTGCTC AAGGACCCAG AGGAGAATCT GGTACCCCTG GATCTCCTGG ACCTGCTGGA 
GCTTCTGGTA ACCCTGGTAC TGATGGTATT CCTGGCGCCA AAGGTTCATC TGGTGCTCCT 
GGTATTGCTG GTGCCCCTGG TTTCCCTGGA CCACGTGGTC CTCCAGGACC TCAGGGAGCT 
ACTGGTCCTC TTGGTCCCAA AGGCCAGACT GGTGACCCCG GTGTTGCAGG TTTCAAGGGT 
GAACATGGTC CCAAAGGTGA AATCGGGTCT GCAGGTCCTC AGGGTGCTCC TGGCCCAGCT 
GGTGAAGAAG GCAAGAGAGG AGCCCGTGGT GAACCTGGTG CTGCTGGACC CCTTGGTCCT 
CCTGGAGAGA GAGGTGCTCC TGGTAATCGT GGTTTCCCTG GTCAAGATGG TCTTGCTGGT 
CCTAAGGGTG CTCCTGGTGA ACGTGGTGTT CCAGGTCTTG GTGGACCAAA GGGTGCTAAT 
GGTGATCCTG GCCGTCCTGG CGAACCTGGT CTCCCTGGTG CTAGGGGTCT TACTGGCCGT 
CCTGGTGATG CTGGTCCTCA GGGAAAAGTT GGGCCCTCTG GTGCTTCTGG TGAAGATGGT 
CGTCCTGGAC CTCCTGGGCC ACAAGGTGCT CGTGGTCAGC CTGGTGTTAT GGGTTTCCCT 
GGGCCTAAAG GTGCCAATGG CGAACCTGGC AAAGCTGGTG AGAAAGGACT GCTTGGTGCT 
CCTGGTCTGA GGGGTCTGCC TGGAAAAGAT GGTGAAACTG GTGCTCAAGG TCCCAATGGT 
CCAGCTGGAC CTGCTGGTGA AAGAGGTGAA CAAGGACCTC CTGGCCCATC TGGCTTCCAG 
GGACTTCCTG GACCTCCCGG TTCTCCCGGA GAAGGTGGCA AACCAGGTGA TCAGGGTGTT 
CCCGGTGAAG CAGGTGCCCC TGGTCTTGTT GGCCCAAGAG GTGAGCGTGG TTTCCCAGGT 
GAACGTGGTT CTTCAGGTCC TCAAGGTCTT CAGGGTCCTC GTGGTCTTCC TGGAACTCCT 
GGTACTGATG GTCCCAAGGG TGCAACTGGT CCATCTGGTC CCAATGGTGC CCAAGGTCCT 
CCTGGTCTTC AAGGTATGCC TGGTGAGAGA GGAGCTGCTG GTATTTCTGG ACCCAAGGGT 
GATAGAGGTG ATACTGGTGA GAAAGGCCCA GAGGGTGCTC CTGGTAAAGA TGGTTCAAGA 
GGTTTGACTG GTCCAATTGG TCCCCCTGGT CCATCTGGTC CTAATGGTGA GAAGGGAGAA 
TCTGGTCCTT CCGGTCCAGC TGGTATTGTT GGTGCCCGTG GTGCCCCTGG TGATCGTGGT 
GAGACTGGCC CTCCAGGTCC TGCTGGCTTT GCTGGTCCTC CTGGTGCTGA TGGTCAAGCT 
GGTCTTAAGG GTGATCAAGG TGAATCTGGC CAGAAGGGTG ATGCTGGTGC TCCTGGTCCC 
CAGGGTCCAT CTGGTGCTCC TGGCCCACAG GGCCCAACTG GTGTTAATGG TCCTAAGGGA 
GCTCGTGGTG CTCAAGGTCC TCCTGGTGCT ACTGGATTCC CTGGTGCTGC TGGAAGAGTT 
GGTCCCCCTG GTCCCAATGG TAACCCTGGA CCTTCAGGTG CTCCTGGATC TGCTGGTAAA 
GAAGGGCCAA AGGGTGCACG TGGTGATGCT GGTCCTACTG GTCGTGCTGG TGATCCAGGT 
CTTCAAGGTC CTGCTGGTGT TCCTGGAGAG AAGGGAGAGT CTGGTGAAGA TGGTCCTTCT 
GGTCCAGATG GTCCCCCAGG TCCTCAAGGT TTGTCTGGAC AGCGTGGTAT TGTTGGTCTG 
CCAGGTCAGC GTGGTGAAAG AGGATTCCCT GGACTTCCTG GTCCATCTGG TGAACCTGGA 
AAACAAGGAG GCCCTGGATC TGCTGGAGAC CGTGGACCCC CTGGCCCTGT TGGCCCACCT 
GGTTTGACTG GACCTGCTGG AGAGCCTGGA CGTGAAGGTA ACGCTGGATC TGATGGCCCA 
CCTGGTCGTG ATGGTGCTAC TGGAATTAAG GGTGATCGTG GTGAGACTGG TCCTCTTGGT 
GCTCCTGGTG CTCCCGGTGC CCCTGGTGCT CCTGGACCTG TTGGCCCAAC TGGAAAACAA 
GGAGACAGAG GAGAATCTGG ACCCCAAGGT CCACTTGGTC CATCTGGTCC TGCTGGTGCT 
CGTGGCTTGC CTGGTCCTCA AGGTCCACGT GGTGATAAGG GTGAAGCTGG TGAGGCAGGA 
GAGAGAGGAC AGAAAGGTCA CAGAGGATTC ACTGGTCTTC AGGGTCTGCC AGGTCCCCCA 
GGTACTGCTG GTGACCAAGG TGCTTCTGGT CCTGCCGGTC CTGGTGGTCC TAGAGGTCCC 
CCTGGCCCTG TTGGTCCTTC TGGCAAAGAT GGCTCTAATG GTCTTCCTGG TCCAATTGGT 
CCACCTGGTC CACGCGGTCG CGGTGGTGAA ACTGGTCCTG CTGGTCCACC TGGTCAACCT 
GGCCCACCTG GTCCCCCTGG TCCTCCTGGC CCTGGAATTG ACATGTCTGC TTTCGCCGGC 
CTATCTCAAC CTGAAAAAGG ACCCGACCCA ATGCGTTACA TGCGTGCTGA CCAAGCCTCC 
AGCTCTGTCC CTCAGCGTGA TGTTGATGTT GAAGCCACTT TGAAATCCCT TAATAACCAG 
ATCGAAAGCA TCCGCAGCCC AGATGGTACC AAGAAGAACC CAGCCCGCAC ATGCCGTGAC 
CTGAAACTCT GCCATCCTGA ATGGAAGAGC GGTGACTATT GGATCGATCC CAACCAGGGC 
TGCACTGTAG ATGCTATCAA AGTCTTCTGC AACATGGAGA CTGGAGAGAC CTGTGTATAC 
CCCAACCCAT CAAAGATCCC CAAGAAGAAC TGGTGGTCCG CCAAGGGCAA GGAAAAGAAA 
CACATTTGGT TTGGAGAGAC AATCAATGGT GGCTTCCAAT TTAGCTATGG AGATGACAGC 
TCAGCACCCA ACACTGCCAA CATTCAGATG ACCTTCCTGC GTCTGCTCTC CACTGATGCA 
ACCCAGAACA TCACCTATCA CTGCAAGAAC AGCATTGCAT TTATGGATGA AGCTTCAGGC 
AACCTTAAGA AGGCTGTTCT ACTCCAAGGA TCCAATGATG TAGAAATCAG AGCTGAGGGC 
AACAGTAGAT TCACATACAA TGCCTTGGAA GATGGCTGCA AGAAACACAC TGGCAAATGG 
AGCAAGACAG TAATTGAATA CAGGACACAG AAAACATCCC GCCTGCCCAT CGTAGACATT 
GCACCTATGG ATATTGGTGG CGCTGATCAG GAATTTGGTG TTGACATTGG CCCAGTCTGC 
TTCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col2a1 ( NM_203889.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_989220.1 Xenopus tropicalis collagen, type II, alpha 1 (col2a1), mRNA.

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CloneID	OXa21643
Clone ID Related Accession (Same CDS sequence)	NM_203889.1
Accession Version	NM_203889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(II) chain precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC063191.1. ##Evidence-Data-START## Transcript exon combination :: BC063191.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028291, SAMD00028292 [ECO:0000348] ##Evidence-Data-END##

ATGTTTAGCT TTGTGGATTC AAGGACTCTA GTGCTGTTCG CAGCCACACA AGTCATTTTA 
TTAGCGGTTG TACGATGCCA AGATGAAGAA GATGTCCTGG CCACAGGCAG CTGCGTGCAG 
CATGGGCAGA GGTATAGTGA TAAAGATGTG TGGAAACCAG AGCCCTGCCA AATCTGTGTC 
TGTGACACTG GGAATGTTCT CTGCGATGAG ATAATCTGCG AAGATCCCAA AGATTGCCCC 
AATGCTGAGA TCCCCTTCGG AGAGTGCTGC CCCATCTGCC CAACTGAGCA ATCTTCTACC 
TCCAGTGGGC AAGGAGTACT CAAGGGCCAG AAAGGTGAAC CCGGTGATAT TAAAGATGTT 
GTAGGACCAA AAGGACCACC TGGACCACAG GGACCTTCTG GTGAACAAGG ACCTAGAGGA 
GATCGTGGAG ACAAGGGCGA GAAAGGTGCT CCTGGACCCC GTGGAAGAGA TGGCGAACCC 
GGTACTCCTG GGAACCCAGG ACCTGTTGGA CCCCCTGGAC CTCCTGGACC CCCTGGCCTT 
GGCGGAAACT TTGCTGCTCA AATGACAGGA GGATTTGATG AAAAAGCTGG TGGTGCACAA 
ATGGGAGTTA TGCAAGGACC AATGGGCCCT ATGGGACCAA GAGGACCTCC CGGCCCAACA 
GGAGCTCCTG GACCTCAAGG TTTCCAAGGC AATCCCGGTG AACCTGGTGA ACCTGGCGCT 
GGTGGTCCAA TGGGTCCCCG TGGTCCTCCT GGCCCTGCTG GAAAGCCTGG TGATGATGGT 
GAAGCTGGCA AACCTGGCAA ATCTGGTGAG CGTGGACCCC CAGGACCACA GGGAGCTAGA 
GGTTTCCCTG GAACCCCAGG ACTTCCAGGA GTAAAAGGAC ACAGGGGTTA CCCAGGTCTT 
GATGGGTCAA AGGGTGAAGC TGGTGCTGCT GGTGCCAAGG GAGAAGGTGG TGCTACTGGT 
GAAGCTGGAT CTCCTGGTCC AATGGGTCCC CGTGGTCTTC CAGGTGAGAG AGGACGCCCT 
GGAGCTTCTG GTGCTGCTGG TGCTCGTGGT AATGATGGTC TTCCTGGCCC TGCTGGTCCC 
CCAGGACCTG TTGGCCCTGC TGGTGCTCCC GGTTTCCCTG GTGCTCCTGG TTCAAAGGGT 
GAAGCTGGCC CAACTGGTGC TCGTGGACCT GAGGGTGCTC AAGGACCCAG AGGAGAATCT 
GGTACCCCTG GATCTCCTGG ACCTGCTGGA GCTTCTGGTA ACCCTGGTAC TGATGGTATT 
CCTGGCGCCA AAGGTTCATC TGGTGCTCCT GGTATTGCTG GTGCCCCTGG TTTCCCTGGA 
CCACGTGGTC CTCCAGGACC TCAGGGAGCT ACTGGTCCTC TTGGTCCCAA AGGCCAGACT 
GGTGACCCCG GTGTTGCAGG TTTCAAGGGT GAACATGGTC CCAAAGGTGA AATCGGGTCT 
GCAGGTCCTC AGGGTGCTCC TGGCCCAGCT GGTGAAGAAG GCAAGAGAGG AGCCCGTGGT 
GAACCTGGTG CTGCTGGACC CCTTGGTCCT CCTGGAGAGA GAGGTGCTCC TGGTAATCGT 
GGTTTCCCTG GTCAAGATGG TCTTGCTGGT CCTAAGGGTG CTCCTGGTGA ACGTGGTGTT 
CCAGGTCTTG GTGGACCAAA GGGTGCTAAT GGTGATCCTG GCCGTCCTGG CGAACCTGGT 
CTCCCTGGTG CTAGGGGTCT TACTGGCCGT CCTGGTGATG CTGGTCCTCA GGGAAAAGTT 
GGGCCCTCTG GTGCTTCTGG TGAAGATGGT CGTCCTGGAC CTCCTGGGCC ACAAGGTGCT 
CGTGGTCAGC CTGGTGTTAT GGGTTTCCCT GGGCCTAAAG GTGCCAATGG CGAACCTGGC 
AAAGCTGGTG AGAAAGGACT GCTTGGTGCT CCTGGTCTGA GGGGTCTGCC TGGAAAAGAT 
GGTGAAACTG GTGCTCAAGG TCCCAATGGT CCAGCTGGAC CTGCTGGTGA AAGAGGTGAA 
CAAGGACCTC CTGGCCCATC TGGCTTCCAG GGACTTCCTG GACCTCCCGG TTCTCCCGGA 
GAAGGTGGCA AACCAGGTGA TCAGGGTGTT CCCGGTGAAG CAGGTGCCCC TGGTCTTGTT 
GGCCCAAGAG GTGAGCGTGG TTTCCCAGGT GAACGTGGTT CTTCAGGTCC TCAAGGTCTT 
CAGGGTCCTC GTGGTCTTCC TGGAACTCCT GGTACTGATG GTCCCAAGGG TGCAACTGGT 
CCATCTGGTC CCAATGGTGC CCAAGGTCCT CCTGGTCTTC AAGGTATGCC TGGTGAGAGA 
GGAGCTGCTG GTATATCTGG ACCCAAGGGT GATAGAGGTG ATACTGGTGA GAAAGGCCCA 
GAGGGTGCTC CTGGTAAAGA TGGTTCAAGA GGTTTGACTG GTCCAATTGG CCCCCCTGGT 
CCATCTGGTC CTAATGGTGA GAAGGGAGAA TCTGGTCCTT CCGGTCCAGC TGGTATTGTT 
GGTGCCCGTG GTGCCCCTGG TGATCGTGGT GAGACTGGCC CTCCAGGTCC TGCTGGCTTT 
GCTGGTCCTC CTGGTGCTGA TGGTCAAGCT GGTCTTAAGG GTGATCAAGG TGAATCTGGC 
CAGAAGGGTG ATGCTGGTGC TCCTGGTCCC CAGGGTCCAT CTGGTGCTCC TGGCCCACAG 
GGCCCAACTG GTGTTAATGG TCCTAAGGGA GCTCGCGGTG CTCAAGGTCC TCCTGGTGCT 
ACTGGATTCC CTGGTGCTGC TGGAAGAGTT GGTCCCCCTG GTCCCAATGG TAACCCTGGA 
CCTTCAGGTG CTCCTGGATC TGCTGGTAAA GAAGGGCCAA AGGGTGCACG TGGTGATGCT 
GGTCCTACTG GTCGTGCTGG TGATCCAGGT CTTCAAGGTC CTGCTGGTGT TCCTGGAGAG 
AAGGGAGAGT CTGGTGAAGA TGGTCCTTCT GGTCCAGATG GTCCCCCAGG TCCTCAAGGT 
TTGTCTGGAC AGCGTGGTAT TGTTGGTCTG CCAGGTCAGC GTGGTGAAAG AGGATTCCCT 
GGACTTCCTG GTCCATCTGG TGAACCTGGA AAACAAGGAG GCCCTGGATC TGCTGGAGAC 
CGTGGACCCC CTGGCCCTGT TGGCCCACCT GGTTTGACTG GACCTGCTGG AGAGCCTGGA 
CGTGAAGGTA ACGCTGGATC TGATGGCCCA CCTGGTCGTG ATGGTGCTAC TGGAATTAAG 
GGTGATCGTG GTGAGACTGG TCCTCTTGGT GCTCCTGGTG CTCCCGGTGC CCCTGGTGCT 
CCTGGACCTG TTGGCCCAAC TGGAAAACAA GGAGACAGAG GAGAATCTGG ACCCCAAGGT 
CCACTTGGTC CATCTGGTCC TGCTGGTGCT CGTGGCTTGC CTGGTCCTCA AGGTCCACGT 
GGTGATAAGG GTGAAGCTGG TGAGGCAGGA GAGAGAGGAC AGAAAGGTCA CAGAGGATTC 
ACTGGTCTTC AGGGTCTGCC AGGTCCCCCA GGTACTGCTG GTGACCAAGG TGCTTCTGGT 
CCTGCCGGTC CTGGTGGTCC TAGAGGTCCC CCTGGCCCTG TTGGTCCTTC TGGCAAAGAT 
GGCTCTAATG GTCTTCCTGG TCCAATTGGT CCACCTGGTC CACGCGGTCG CGGTGGTGAA 
ACTGGTCCTG CTGGTCCACC TGGTCAACCT GGCCCACCTG GTCCCCCTGG TCCTCCTGGC 
CCTGGAATTG ACATGTCTGC TTTCGCCGGC CTATCTCAAC CTGAAAAAGG ACCCGACCCA 
ATGCGTTACA TGCGTGCTGA CCAAGCCTCC AGCTCTGTCC CTCAGCGTGA TGTTGATGTT 
GAAGCCACTT TGAAATCCCT TAATAACCAG ATCGAAAGCA TCCGCAGCCC AGATGGTACC 
AAGAAGAACC CAGCCCGCAC ATGCCGTGAC CTGAAACTCT GCCATCCTGA ATGGAAGAGC 
GGTGACTATT GGATCGATCC CAACCAGGGC TGCACTGTAG ATGCTATCAA AGTCTTCTGC 
AACATGGAGA CTGGAGAGAC CTGTGTATAC CCCAACCCAT CAAAGATCCC CAAGAAGAAC 
TGGTGGTCCG CCAAGGGCAA GGAAAAGAAA CACATTTGGT TTGGAGAGAC AATCAATGGT 
GGCTTCCAAT TTAGCTATGG AGATGACAGC TCAGCACCCA ACACTGCCAA CATTCAGATG 
ACCTTCCTGC GTCTGCTCTC CACTGATGCA ACCCAGAACA TCACCTATCA CTGCAAGAAC 
AGCATTGCAT TTATGGATGA AGCTTCAGGC AACCTTAAGA AGGCTGTTCT ACTCCAAGGA 
TCCAATGATG TAGAAATCAG AGCTGAGGGC AACAGTAGAT TCACATACAA TGCCTTGGAA 
GATGGCTGCA AGAAACACAC TGGCAAATGG AGCAAGACAG TAATTGAATA CAGGACACAG 
AAAACATCTC GCCTGCCCAT CGTAGACATT GCACCTATGG ATATTGGTGG CGCTGATCAG 
GAATTTGGTG TTGACATTGG CCCAGTCTGC TTCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989220.1
CDS	172..4650
Translation	MFSFVDSRTLVLFAATQVILLAVVRCQDEEDVLATGSCVQHGQRYSDKDVWKPEPCQICVCDTGNVLCDEIICEDPKDCPNAEIPFGECCPICPTEQSSTSSGQGVLKGQKGEPGDIKDVVGPKGPPGPQGPSGEQGPRGDRGDKGEKGAPGPRGRDGEPGTPGNPGPVGPPGPPGPPGLGGNFAAQMTGGFDEKAGGAQMGVMQGPMGPMGPRGPPGPTGAPGPQGFQGNPGEPGEPGAGGPMGPRGPPGPAGKPGDDGEAGKPGKSGERGPPGPQGARGFPGTPGLPGVKGHRGYPGLDGSKGEAGAAGAKGEGGATGEAGSPGPMGPRGLPGERGRPGASGAAGARGNDGLPGPAGPPGPVGPAGAPGFPGAPGSKGEAGPTGARGPEGAQGPRGESGTPGSPGPAGASGNPGTDGIPGAKGSSGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQTGDPGVAGFKGEHGPKGEIGSAGPQGAPGPAGEEGKRGARGEPGAAGPLGPPGERGAPGNRGFPGQDGLAGPKGAPGERGVPGLGGPKGANGDPGRPGEPGLPGARGLTGRPGDAGPQGKVGPSGASGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLLGAPGLRGLPGKDGETGAQGPNGPAGPAGERGEQGPPGPSGFQGLPGPPGSPGEGGKPGDQGVPGEAGAPGLVGPRGERGFPGERGSSGPQGLQGPRGLPGTPGTDGPKGATGPSGPNGAQGPPGLQGMPGERGAAGISGPKGDRGDTGEKGPEGAPGKDGSRGLTGPIGPPGPSGPNGEKGESGPSGPAGIVGARGAPGDRGETGPPGPAGFAGPPGADGQAGLKGDQGESGQKGDAGAPGPQGPSGAPGPQGPTGVNGPKGARGAQGPPGATGFPGAAGRVGPPGPNGNPGPSGAPGSAGKEGPKGARGDAGPTGRAGDPGLQGPAGVPGEKGESGEDGPSGPDGPPGPQGLSGQRGIVGLPGQRGERGFPGLPGPSGEPGKQGGPGSAGDRGPPGPVGPPGLTGPAGEPGREGNAGSDGPPGRDGATGIKGDRGETGPLGAPGAPGAPGAPGPVGPTGKQGDRGESGPQGPLGPSGPAGARGLPGPQGPRGDKGEAGEAGERGQKGHRGFTGLQGLPGPPGTAGDQGASGPAGPGGPRGPPGPVGPSGKDGSNGLPGPIGPPGPRGRGGETGPAGPPGQPGPPGPPGPPGPGIDMSAFAGLSQPEKGPDPMRYMRADQASSSVPQRDVDVEATLKSLNNQIESIRSPDGTKKNPARTCRDLKLCHPEWKSGDYWIDPNQGCTVDAIKVFCNMETGETCVYPNPSKIPKKNWWSAKGKEKKHIWFGETINGGFQFSYGDDSSAPNTANIQMTFLRLLSTDATQNITYHCKNSIAFMDEASGNLKKAVLLQGSNDVEIRAEGNSRFTYNALEDGCKKHTGKWSKTVIEYRTQKTSRLPIVDIAPMDIGGADQEFGVDIGPVCFL

Target ORF information:

RefSeq Version	NM_203889.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type II, alpha 1 (col2a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_203889.1

ORF Insert Sequence:

ATGTTTAGCT TTGTGGATTC AAGGACTCTA GTGCTGTTCG CAGCCACACA AGTCATTTTA 
TTAGCGGTTG TACGATGCCA AGATGAAGAA GATGTCCTGG CCACAGGCAG CTGCGTGCAG 
CATGGGCAGA GGTATAGTGA TAAAGATGTG TGGAAACCAG AGCCCTGCCA AATCTGTGTC 
TGTGACACTG GGAATGTTCT CTGCGATGAG ATAATCTGCG AAGATCCCAA AGATTGCCCC 
AATGCTGAGA TCCCCTTCGG AGAGTGCTGC CCCATCTGCC CAACTGAGCA ATCTTCTACC 
TCCAGTGGGC AAGGAGTACT CAAGGGCCAG AAAGGTGAAC CCGGTGATAT TAAAGATGTT 
GTAGGACCAA AAGGACCACC TGGACCACAG GGACCTTCTG GTGAACAAGG ACCTAGAGGA 
GATCGTGGAG ACAAGGGCGA GAAAGGTGCT CCTGGACCCC GTGGAAGAGA TGGCGAACCC 
GGTACTCCTG GGAACCCAGG ACCTGTTGGA CCCCCTGGAC CTCCTGGACC CCCTGGCCTT 
GGCGGAAACT TTGCTGCTCA AATGACAGGA GGATTTGATG AAAAAGCTGG TGGTGCACAA 
ATGGGAGTTA TGCAAGGACC AATGGGCCCT ATGGGACCAA GAGGACCTCC CGGCCCAACA 
GGAGCTCCTG GACCTCAAGG TTTCCAAGGC AATCCCGGTG AACCTGGTGA ACCTGGCGCT 
GGTGGTCCAA TGGGTCCCCG TGGTCCTCCT GGCCCTGCTG GAAAGCCTGG TGATGATGGT 
GAAGCTGGCA AACCTGGCAA ATCTGGTGAG CGTGGACCCC CAGGACCACA GGGAGCTAGA 
GGTTTCCCTG GAACCCCAGG ACTTCCAGGA GTAAAAGGAC ACAGGGGTTA CCCAGGTCTT 
GATGGGTCAA AGGGTGAAGC TGGTGCTGCT GGTGCCAAGG GAGAAGGTGG TGCTACTGGT 
GAAGCTGGAT CTCCTGGTCC AATGGGTCCC CGTGGTCTTC CAGGTGAGAG AGGACGCCCT 
GGAGCTTCTG GTGCTGCTGG TGCTCGTGGT AATGATGGTC TTCCTGGCCC TGCTGGTCCC 
CCAGGACCTG TTGGCCCTGC TGGTGCTCCC GGTTTCCCTG GTGCTCCTGG TTCAAAGGGT 
GAAGCTGGCC CAACTGGTGC TCGTGGACCT GAGGGTGCTC AAGGACCCAG AGGAGAATCT 
GGTACCCCTG GATCTCCTGG ACCTGCTGGA GCTTCTGGTA ACCCTGGTAC TGATGGTATT 
CCTGGCGCCA AAGGTTCATC TGGTGCTCCT GGTATTGCTG GTGCCCCTGG TTTCCCTGGA 
CCACGTGGTC CTCCAGGACC TCAGGGAGCT ACTGGTCCTC TTGGTCCCAA AGGCCAGACT 
GGTGACCCCG GTGTTGCAGG TTTCAAGGGT GAACATGGTC CCAAAGGTGA AATCGGGTCT 
GCAGGTCCTC AGGGTGCTCC TGGCCCAGCT GGTGAAGAAG GCAAGAGAGG AGCCCGTGGT 
GAACCTGGTG CTGCTGGACC CCTTGGTCCT CCTGGAGAGA GAGGTGCTCC TGGTAATCGT 
GGTTTCCCTG GTCAAGATGG TCTTGCTGGT CCTAAGGGTG CTCCTGGTGA ACGTGGTGTT 
CCAGGTCTTG GTGGACCAAA GGGTGCTAAT GGTGATCCTG GCCGTCCTGG CGAACCTGGT 
CTCCCTGGTG CTAGGGGTCT TACTGGCCGT CCTGGTGATG CTGGTCCTCA GGGAAAAGTT 
GGGCCCTCTG GTGCTTCTGG TGAAGATGGT CGTCCTGGAC CTCCTGGGCC ACAAGGTGCT 
CGTGGTCAGC CTGGTGTTAT GGGTTTCCCT GGGCCTAAAG GTGCCAATGG CGAACCTGGC 
AAAGCTGGTG AGAAAGGACT GCTTGGTGCT CCTGGTCTGA GGGGTCTGCC TGGAAAAGAT 
GGTGAAACTG GTGCTCAAGG TCCCAATGGT CCAGCTGGAC CTGCTGGTGA AAGAGGTGAA 
CAAGGACCTC CTGGCCCATC TGGCTTCCAG GGACTTCCTG GACCTCCCGG TTCTCCCGGA 
GAAGGTGGCA AACCAGGTGA TCAGGGTGTT CCCGGTGAAG CAGGTGCCCC TGGTCTTGTT 
GGCCCAAGAG GTGAGCGTGG TTTCCCAGGT GAACGTGGTT CTTCAGGTCC TCAAGGTCTT 
CAGGGTCCTC GTGGTCTTCC TGGAACTCCT GGTACTGATG GTCCCAAGGG TGCAACTGGT 
CCATCTGGTC CCAATGGTGC CCAAGGTCCT CCTGGTCTTC AAGGTATGCC TGGTGAGAGA 
GGAGCTGCTG GTATATCTGG ACCCAAGGGT GATAGAGGTG ATACTGGTGA GAAAGGCCCA 
GAGGGTGCTC CTGGTAAAGA TGGTTCAAGA GGTTTGACTG GTCCAATTGG CCCCCCTGGT 
CCATCTGGTC CTAATGGTGA GAAGGGAGAA TCTGGTCCTT CCGGTCCAGC TGGTATTGTT 
GGTGCCCGTG GTGCCCCTGG TGATCGTGGT GAGACTGGCC CTCCAGGTCC TGCTGGCTTT 
GCTGGTCCTC CTGGTGCTGA TGGTCAAGCT GGTCTTAAGG GTGATCAAGG TGAATCTGGC 
CAGAAGGGTG ATGCTGGTGC TCCTGGTCCC CAGGGTCCAT CTGGTGCTCC TGGCCCACAG 
GGCCCAACTG GTGTTAATGG TCCTAAGGGA GCTCGCGGTG CTCAAGGTCC TCCTGGTGCT 
ACTGGATTCC CTGGTGCTGC TGGAAGAGTT GGTCCCCCTG GTCCCAATGG TAACCCTGGA 
CCTTCAGGTG CTCCTGGATC TGCTGGTAAA GAAGGGCCAA AGGGTGCACG TGGTGATGCT 
GGTCCTACTG GTCGTGCTGG TGATCCAGGT CTTCAAGGTC CTGCTGGTGT TCCTGGAGAG 
AAGGGAGAGT CTGGTGAAGA TGGTCCTTCT GGTCCAGATG GTCCCCCAGG TCCTCAAGGT 
TTGTCTGGAC AGCGTGGTAT TGTTGGTCTG CCAGGTCAGC GTGGTGAAAG AGGATTCCCT 
GGACTTCCTG GTCCATCTGG TGAACCTGGA AAACAAGGAG GCCCTGGATC TGCTGGAGAC 
CGTGGACCCC CTGGCCCTGT TGGCCCACCT GGTTTGACTG GACCTGCTGG AGAGCCTGGA 
CGTGAAGGTA ACGCTGGATC TGATGGCCCA CCTGGTCGTG ATGGTGCTAC TGGAATTAAG 
GGTGATCGTG GTGAGACTGG TCCTCTTGGT GCTCCTGGTG CTCCCGGTGC CCCTGGTGCT 
CCTGGACCTG TTGGCCCAAC TGGAAAACAA GGAGACAGAG GAGAATCTGG ACCCCAAGGT 
CCACTTGGTC CATCTGGTCC TGCTGGTGCT CGTGGCTTGC CTGGTCCTCA AGGTCCACGT 
GGTGATAAGG GTGAAGCTGG TGAGGCAGGA GAGAGAGGAC AGAAAGGTCA CAGAGGATTC 
ACTGGTCTTC AGGGTCTGCC AGGTCCCCCA GGTACTGCTG GTGACCAAGG TGCTTCTGGT 
CCTGCCGGTC CTGGTGGTCC TAGAGGTCCC CCTGGCCCTG TTGGTCCTTC TGGCAAAGAT 
GGCTCTAATG GTCTTCCTGG TCCAATTGGT CCACCTGGTC CACGCGGTCG CGGTGGTGAA 
ACTGGTCCTG CTGGTCCACC TGGTCAACCT GGCCCACCTG GTCCCCCTGG TCCTCCTGGC 
CCTGGAATTG ACATGTCTGC TTTCGCCGGC CTATCTCAAC CTGAAAAAGG ACCCGACCCA 
ATGCGTTACA TGCGTGCTGA CCAAGCCTCC AGCTCTGTCC CTCAGCGTGA TGTTGATGTT 
GAAGCCACTT TGAAATCCCT TAATAACCAG ATCGAAAGCA TCCGCAGCCC AGATGGTACC 
AAGAAGAACC CAGCCCGCAC ATGCCGTGAC CTGAAACTCT GCCATCCTGA ATGGAAGAGC 
GGTGACTATT GGATCGATCC CAACCAGGGC TGCACTGTAG ATGCTATCAA AGTCTTCTGC 
AACATGGAGA CTGGAGAGAC CTGTGTATAC CCCAACCCAT CAAAGATCCC CAAGAAGAAC 
TGGTGGTCCG CCAAGGGCAA GGAAAAGAAA CACATTTGGT TTGGAGAGAC AATCAATGGT 
GGCTTCCAAT TTAGCTATGG AGATGACAGC TCAGCACCCA ACACTGCCAA CATTCAGATG 
ACCTTCCTGC GTCTGCTCTC CACTGATGCA ACCCAGAACA TCACCTATCA CTGCAAGAAC 
AGCATTGCAT TTATGGATGA AGCTTCAGGC AACCTTAAGA AGGCTGTTCT ACTCCAAGGA 
TCCAATGATG TAGAAATCAG AGCTGAGGGC AACAGTAGAT TCACATACAA TGCCTTGGAA 
GATGGCTGCA AGAAACACAC TGGCAAATGG AGCAAGACAG TAATTGAATA CAGGACACAG 
AAAACATCTC GCCTGCCCAT CGTAGACATT GCACCTATGG ATATTGGTGG CGCTGATCAG 
GAATTTGGTG TTGACATTGG CCCAGTCTGC TTCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col2a1 ( XM_012956661.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812115.1 Xenopus tropicalis collagen, type II, alpha 1 (col2a1), transcript variant X1, mRNA.

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CloneID	OXa26091
Clone ID Related Accession (Same CDS sequence)	XM_012956661.2 , XM_012956661.3
Accession Version	XM_012956661.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(II) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956661.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGCT TTGTGGATTC AAGGACTCTA GTGCTGTTCG CAGCCACACA AGTCATTTTA 
TTAGCGGTTG TACGATGCCA AGATGAAGAA GATGTCCTGG CCACAGGCAG CTGCGTGCAG 
CATGGGCAGA GGTATAGTGA TAAAGATGTG TGGAAACCAG AGCCCTGCCA AATCTGTGTC 
TGTGACACTG GGAATGTTCT CTGCGATGAG ATAATCTGCG AAGATCCCAA AGATTGCCCC 
AATGCTGAGA TCCCCTTCGG AGAGTGCTGC CCCATCTGCC CAACTGAGCA ATCTTCTACC 
TCCAGTGGGC AAGGAGTACT CAAGGGCCAG AAAGGTGAAC CCGGTGATAT TAAAGATGTT 
GTAGGACCAA AAGGACCACC TGGACCACAG GGACCTTCTG GTGAACAAGG ACCTAGAGGA 
GATCGTGGAG ACAAGGGCGA GAAAGGTGCT CCTGGACCCC GTGGAAGAGA TGGCGAACCC 
GGTACTCCTG GGAACCCAGG ACCTGTTGGA CCCCCTGGAC CTCCTGGACC CCCTGGCCTT 
GGCGGAAACT TTGCTGCTCA AATGACAGGA GGATTTGATG AAAAAGCTGG TGGTGCACAA 
ATGGGAGTTA TGCAAGGACC AATGGGCCCT ATGGGACCAA GAGGACCTCC CGGCCCAACA 
GGAGCTCCTG GACCTCAAGG TTTCCAAGGC AATCCCGGTG AACCTGGTGA ACCTGGCGCT 
GGTGGTCCAA TGGGTCCCCG TGGTCCTCCT GGCCCTGCTG GAAAGCCTGG TGATGATGGT 
GAAGCTGGCA AACCTGGCAA ATCTGGTGAG CGTGGACCCC CAGGACCACA GGGAGCTAGA 
GGTTTCCCTG GAACCCCAGG ACTTCCAGGA GTAAAAGGAC ACAGGGGTTA CCCAGGTCTT 
GATGGGTCAA AGGGTGAAGC TGGTGCTGCT GGTGCCAAGG GAGAAGGTGG TGCTACTGGT 
GAAGCTGGAT CTCCTGGTCC AATGGGTCCC CGTGGTCTTC CAGGTGAGAG AGGACGCCCT 
GGAGCTTCTG GTGCTGCTGG TGCTCGTGGT AATGATGGTC TTCCTGGCCC TGCTGGTCCC 
CCAGGACCTG TTGGCCCTGC TGGTGCTCCT GGTTTCCCTG GTGCTCCTGG TTCAAAGGGT 
GAAGCTGGCC CAACTGGTGC TCGTGGACCT GAGGGTGCTC AAGGACCCAG AGGAGAATCT 
GGTACCCCTG GATCTCCTGG ACCTGCTGGA GCTTCTGGTA ACCCTGGTAC TGATGGTATT 
CCTGGCGCCA AAGGTTCATC TGGTGCTCCT GGTATTGCTG GTGCCCCTGG TTTCCCTGGA 
CCACGTGGTC CTCCAGGACC TCAGGGAGCT ACTGGTCCTC TTGGTCCCAA AGGCCAGACT 
GGTGACCCCG GTGTTGCAGG TTTCAAGGGT GAACATGGTC CCAAAGGTGA AATCGGGTCT 
GCAGGTCCTC AGGGTGCTCC TGGCCCAGCT GGTGAAGAAG GCAAGAGAGG AGCCCGTGGT 
GAACCTGGTG CTGCTGGACC CCTTGGTCCT CCTGGAGAGA GAGGTGCTCC TGGTAATCGT 
GGTTTCCCTG GTCAAGATGG TCTTGCTGGT CCTAAGGGTG CTCCTGGTGA ACGTGGTGTT 
CCAGGTCTTG GTGGACCAAA GGGTGCTAAT GGTGATCCTG GCCGTCCTGG CGAACCTGGT 
CTCCCTGGTG CTAGGGGTCT TACTGGCCGT CCTGGTGATG CTGGTCCTCA GGGAAAAGTT 
GGGCCCTCTG GTGCTTCTGG TGAAGATGGT CGTCCTGGAC CTCCTGGGCC ACAAGGTGCT 
CGTGGTCAGC CTGGTGTTAT GGGTTTCCCT GGGCCTAAAG GTGCCAATGG CGAACCTGGC 
AAAGCTGGTG AGAAAGGACT GCTTGGTGCT CCTGGTCTGA GGGGTCTGCC TGGAAAAGAT 
GGTGAAACTG GTGCTCAAGG TCCCAATGGT CCAGCTGGAC CTGCTGGTGA AAGAGGTGAA 
CAAGGACCTC CTGGCCCATC TGGCTTCCAG GGACTTCCTG GACCTCCCGG TTCTCCCGGA 
GAAGGTGGCA AACCAGGTGA TCAGGGTGTT CCCGGTGAAG CAGGTGCCCC TGGTCTTGTT 
GGCCCAAGAG GTGAGCGTGG TTTCCCAGGT GAACGTGGTT CTTCAGGTCC TCAAGGTCTT 
CAGGGTCCTC GTGGTCTTCC TGGAACTCCT GGTACTGATG GTCCCAAGGG TGCAACTGGT 
CCATCTGGTC CCAATGGTGC CCAAGGTCCT CCTGGTCTTC AAGGTATGCC TGGTGAGAGA 
GGAGCTGCTG GTATTTCTGG ACCCAAGGGT GATAGAGGTG ATACTGGTGA GAAAGGCCCA 
GAGGGTGCTC CTGGTAAAGA TGGTTCAAGA GGTTTGACTG GTCCAATTGG TCCCCCTGGT 
CCATCTGGTC CTAATGGTGA GAAGGGAGAA TCTGGTCCTT CCGGTCCAGC TGGTATTGTT 
GGTGCCCGTG GTGCCCCTGG TGATCGTGGT GAGACTGGCC CTCCAGGTCC TGCTGGCTTT 
GCTGGTCCTC CTGGTGCTAC TGGATTCCCT GGTGCTGCTG GAAGAGTTGG TCCCCCTGGT 
CCCAATGGTA ACCCTGGACC TTCAGGTGCT CCTGGATCTG CTGGTAAAGA AGGGCCAAAG 
GGTGCACGTG GTGATGCTGG TCCTACTGGT CGTGCTGGTG ATCCAGGTCT TCAAGGTCCT 
GCTGGTGTTC CTGGAGAGAA GGGAGAGTCT GGTGAAGATG GTCCTTCTGG TCCAGATGGT 
CCCCCAGGTC CTCAAGGTTT GTCTGGACAG CGTGGTATTG TTGGTCTGCC AGGTCAGCGT 
GGTGAAAGAG GATTCCCTGG ACTTCCTGGT CCATCTGGTG AACCTGGAAA ACAAGGAGGC 
CCTGGATCTG CTGGAGACCG TGGACCCCCT GGCCCTGTTG GCCCACCTGG TTTGACTGGA 
CCTGCTGGAG AGCCTGGACG TGAAGGTAAC GCTGGATCTG ATGGCCCACC TGGTCGTGAT 
GGTGCTACTG GAATTAAGGG TGATCGTGGT GAGACTGGTC CTCTTGGTGC TCCTGGTGCT 
CCCGGTGCCC CTGGTGCTCC TGGACCTGTT GGCCCAACTG GAAAACAAGG AGACAGAGGA 
GAATCTGGAC CCCAAGGTCC ACTTGGTCCA TCTGGTCCTG CTGGTGCTCG TGGCTTGCCT 
GGTCCTCAAG GTCCACGTGG TGATAAGGGT GAAGCTGGTG AGGCAGGAGA GAGAGGACAG 
AAAGGTCACA GAGGATTCAC TGGTCTTCAG GGTCTGCCAG GTCCCCCAGG TACTGCTGGT 
GACCAAGGTG CTTCTGGTCC TGCCGGTCCT GGTGGTCCTA GAGGTCCCCC TGGCCCTGTT 
GGTCCTTCTG GCAAAGATGG CTCTAATGGT CTTCCTGGTC CAATTGGTCC ACCTGGTCCA 
CGCGGTCGCG GTGGTGAAAC TGGTCCTGCT GGTCCACCTG GTCAACCTGG CCCACCTGGT 
CCCCCTGGTC CTCCTGGCCC TGGAATTGAC ATGTCTGCTT TCGCCGGCCT ATCTCAACCT 
GAAAAAGGAC CCGACCCAAT GCGTTACATG CGTGCTGACC AAGCCTCCAG CTCTGTCCCT 
CAGCGTGATG TTGATGTTGA AGCCACTTTG AAATCCCTTA ATAACCAGAT CGAAAGCATC 
CGCAGCCCAG ATGGTACCAA GAAGAACCCA GCCCGCACAT GCCGTGACCT GAAACTCTGC 
CATCCTGAAT GGAAGAGCGG TGACTATTGG ATCGATCCCA ACCAGGGCTG CACTGTAGAT 
GCTATCAAAG TCTTCTGCAA CATGGAGACT GGAGAGACCT GTGTATACCC CAACCCATCA 
AAGATCCCCA AGAAGAACTG GTGGTCCGCC AAGGGCAAGG AAAAGAAACA CATTTGGTTT 
GGAGAGACAA TCAATGGTGG CTTCCAATTT AGCTATGGAG ATGACAGCTC AGCACCCAAC 
ACTGCCAACA TTCAGATGAC CTTCCTGCGT CTGCTCTCCA CTGATGCAAC CCAGAACATC 
ACCTATCACT GCAAGAACAG CATTGCATTT ATGGATGAAG CTTCAGGCAA CCTTAAGAAG 
GCTGTTCTAC TCCAAGGATC CAATGATGTA GAAATCAGAG CTGAGGGCAA CAGTAGATTC 
ACATACAATG CCTTGGAAGA TGGCTGCAAG AAACACACTG GCAAATGGAG CAAGACAGTA 
ATTGAATACA GGACACAGAA AACATCCCGC CTGCCCATCG TAGACATTGC ACCTATGGAT 
ATTGGTGGCG CTGATCAGGA ATTTGGTGTT GACATTGGCC CAGTCTGCTT CTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812115.1
CDS	206..4522
Translation	MFSFVDSRTLVLFAATQVILLAVVRCQDEEDVLATGSCVQHGQRYSDKDVWKPEPCQICVCDTGNVLCDEIICEDPKDCPNAEIPFGECCPICPTEQSSTSSGQGVLKGQKGEPGDIKDVVGPKGPPGPQGPSGEQGPRGDRGDKGEKGAPGPRGRDGEPGTPGNPGPVGPPGPPGPPGLGGNFAAQMTGGFDEKAGGAQMGVMQGPMGPMGPRGPPGPTGAPGPQGFQGNPGEPGEPGAGGPMGPRGPPGPAGKPGDDGEAGKPGKSGERGPPGPQGARGFPGTPGLPGVKGHRGYPGLDGSKGEAGAAGAKGEGGATGEAGSPGPMGPRGLPGERGRPGASGAAGARGNDGLPGPAGPPGPVGPAGAPGFPGAPGSKGEAGPTGARGPEGAQGPRGESGTPGSPGPAGASGNPGTDGIPGAKGSSGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQTGDPGVAGFKGEHGPKGEIGSAGPQGAPGPAGEEGKRGARGEPGAAGPLGPPGERGAPGNRGFPGQDGLAGPKGAPGERGVPGLGGPKGANGDPGRPGEPGLPGARGLTGRPGDAGPQGKVGPSGASGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLLGAPGLRGLPGKDGETGAQGPNGPAGPAGERGEQGPPGPSGFQGLPGPPGSPGEGGKPGDQGVPGEAGAPGLVGPRGERGFPGERGSSGPQGLQGPRGLPGTPGTDGPKGATGPSGPNGAQGPPGLQGMPGERGAAGISGPKGDRGDTGEKGPEGAPGKDGSRGLTGPIGPPGPSGPNGEKGESGPSGPAGIVGARGAPGDRGETGPPGPAGFAGPPGATGFPGAAGRVGPPGPNGNPGPSGAPGSAGKEGPKGARGDAGPTGRAGDPGLQGPAGVPGEKGESGEDGPSGPDGPPGPQGLSGQRGIVGLPGQRGERGFPGLPGPSGEPGKQGGPGSAGDRGPPGPVGPPGLTGPAGEPGREGNAGSDGPPGRDGATGIKGDRGETGPLGAPGAPGAPGAPGPVGPTGKQGDRGESGPQGPLGPSGPAGARGLPGPQGPRGDKGEAGEAGERGQKGHRGFTGLQGLPGPPGTAGDQGASGPAGPGGPRGPPGPVGPSGKDGSNGLPGPIGPPGPRGRGGETGPAGPPGQPGPPGPPGPPGPGIDMSAFAGLSQPEKGPDPMRYMRADQASSSVPQRDVDVEATLKSLNNQIESIRSPDGTKKNPARTCRDLKLCHPEWKSGDYWIDPNQGCTVDAIKVFCNMETGETCVYPNPSKIPKKNWWSAKGKEKKHIWFGETINGGFQFSYGDDSSAPNTANIQMTFLRLLSTDATQNITYHCKNSIAFMDEASGNLKKAVLLQGSNDVEIRAEGNSRFTYNALEDGCKKHTGKWSKTVIEYRTQKTSRLPIVDIAPMDIGGADQEFGVDIGPVCFL

Target ORF information:

RefSeq Version	XM_012956661.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type II, alpha 1 (col2a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956661.2

ORF Insert Sequence:

ATGTTTAGCT TTGTGGATTC AAGGACTCTA GTGCTGTTCG CAGCCACACA AGTCATTTTA 
TTAGCGGTTG TACGATGCCA AGATGAAGAA GATGTCCTGG CCACAGGCAG CTGCGTGCAG 
CATGGGCAGA GGTATAGTGA TAAAGATGTG TGGAAACCAG AGCCCTGCCA AATCTGTGTC 
TGTGACACTG GGAATGTTCT CTGCGATGAG ATAATCTGCG AAGATCCCAA AGATTGCCCC 
AATGCTGAGA TCCCCTTCGG AGAGTGCTGC CCCATCTGCC CAACTGAGCA ATCTTCTACC 
TCCAGTGGGC AAGGAGTACT CAAGGGCCAG AAAGGTGAAC CCGGTGATAT TAAAGATGTT 
GTAGGACCAA AAGGACCACC TGGACCACAG GGACCTTCTG GTGAACAAGG ACCTAGAGGA 
GATCGTGGAG ACAAGGGCGA GAAAGGTGCT CCTGGACCCC GTGGAAGAGA TGGCGAACCC 
GGTACTCCTG GGAACCCAGG ACCTGTTGGA CCCCCTGGAC CTCCTGGACC CCCTGGCCTT 
GGCGGAAACT TTGCTGCTCA AATGACAGGA GGATTTGATG AAAAAGCTGG TGGTGCACAA 
ATGGGAGTTA TGCAAGGACC AATGGGCCCT ATGGGACCAA GAGGACCTCC CGGCCCAACA 
GGAGCTCCTG GACCTCAAGG TTTCCAAGGC AATCCCGGTG AACCTGGTGA ACCTGGCGCT 
GGTGGTCCAA TGGGTCCCCG TGGTCCTCCT GGCCCTGCTG GAAAGCCTGG TGATGATGGT 
GAAGCTGGCA AACCTGGCAA ATCTGGTGAG CGTGGACCCC CAGGACCACA GGGAGCTAGA 
GGTTTCCCTG GAACCCCAGG ACTTCCAGGA GTAAAAGGAC ACAGGGGTTA CCCAGGTCTT 
GATGGGTCAA AGGGTGAAGC TGGTGCTGCT GGTGCCAAGG GAGAAGGTGG TGCTACTGGT 
GAAGCTGGAT CTCCTGGTCC AATGGGTCCC CGTGGTCTTC CAGGTGAGAG AGGACGCCCT 
GGAGCTTCTG GTGCTGCTGG TGCTCGTGGT AATGATGGTC TTCCTGGCCC TGCTGGTCCC 
CCAGGACCTG TTGGCCCTGC TGGTGCTCCT GGTTTCCCTG GTGCTCCTGG TTCAAAGGGT 
GAAGCTGGCC CAACTGGTGC TCGTGGACCT GAGGGTGCTC AAGGACCCAG AGGAGAATCT 
GGTACCCCTG GATCTCCTGG ACCTGCTGGA GCTTCTGGTA ACCCTGGTAC TGATGGTATT 
CCTGGCGCCA AAGGTTCATC TGGTGCTCCT GGTATTGCTG GTGCCCCTGG TTTCCCTGGA 
CCACGTGGTC CTCCAGGACC TCAGGGAGCT ACTGGTCCTC TTGGTCCCAA AGGCCAGACT 
GGTGACCCCG GTGTTGCAGG TTTCAAGGGT GAACATGGTC CCAAAGGTGA AATCGGGTCT 
GCAGGTCCTC AGGGTGCTCC TGGCCCAGCT GGTGAAGAAG GCAAGAGAGG AGCCCGTGGT 
GAACCTGGTG CTGCTGGACC CCTTGGTCCT CCTGGAGAGA GAGGTGCTCC TGGTAATCGT 
GGTTTCCCTG GTCAAGATGG TCTTGCTGGT CCTAAGGGTG CTCCTGGTGA ACGTGGTGTT 
CCAGGTCTTG GTGGACCAAA GGGTGCTAAT GGTGATCCTG GCCGTCCTGG CGAACCTGGT 
CTCCCTGGTG CTAGGGGTCT TACTGGCCGT CCTGGTGATG CTGGTCCTCA GGGAAAAGTT 
GGGCCCTCTG GTGCTTCTGG TGAAGATGGT CGTCCTGGAC CTCCTGGGCC ACAAGGTGCT 
CGTGGTCAGC CTGGTGTTAT GGGTTTCCCT GGGCCTAAAG GTGCCAATGG CGAACCTGGC 
AAAGCTGGTG AGAAAGGACT GCTTGGTGCT CCTGGTCTGA GGGGTCTGCC TGGAAAAGAT 
GGTGAAACTG GTGCTCAAGG TCCCAATGGT CCAGCTGGAC CTGCTGGTGA AAGAGGTGAA 
CAAGGACCTC CTGGCCCATC TGGCTTCCAG GGACTTCCTG GACCTCCCGG TTCTCCCGGA 
GAAGGTGGCA AACCAGGTGA TCAGGGTGTT CCCGGTGAAG CAGGTGCCCC TGGTCTTGTT 
GGCCCAAGAG GTGAGCGTGG TTTCCCAGGT GAACGTGGTT CTTCAGGTCC TCAAGGTCTT 
CAGGGTCCTC GTGGTCTTCC TGGAACTCCT GGTACTGATG GTCCCAAGGG TGCAACTGGT 
CCATCTGGTC CCAATGGTGC CCAAGGTCCT CCTGGTCTTC AAGGTATGCC TGGTGAGAGA 
GGAGCTGCTG GTATTTCTGG ACCCAAGGGT GATAGAGGTG ATACTGGTGA GAAAGGCCCA 
GAGGGTGCTC CTGGTAAAGA TGGTTCAAGA GGTTTGACTG GTCCAATTGG TCCCCCTGGT 
CCATCTGGTC CTAATGGTGA GAAGGGAGAA TCTGGTCCTT CCGGTCCAGC TGGTATTGTT 
GGTGCCCGTG GTGCCCCTGG TGATCGTGGT GAGACTGGCC CTCCAGGTCC TGCTGGCTTT 
GCTGGTCCTC CTGGTGCTAC TGGATTCCCT GGTGCTGCTG GAAGAGTTGG TCCCCCTGGT 
CCCAATGGTA ACCCTGGACC TTCAGGTGCT CCTGGATCTG CTGGTAAAGA AGGGCCAAAG 
GGTGCACGTG GTGATGCTGG TCCTACTGGT CGTGCTGGTG ATCCAGGTCT TCAAGGTCCT 
GCTGGTGTTC CTGGAGAGAA GGGAGAGTCT GGTGAAGATG GTCCTTCTGG TCCAGATGGT 
CCCCCAGGTC CTCAAGGTTT GTCTGGACAG CGTGGTATTG TTGGTCTGCC AGGTCAGCGT 
GGTGAAAGAG GATTCCCTGG ACTTCCTGGT CCATCTGGTG AACCTGGAAA ACAAGGAGGC 
CCTGGATCTG CTGGAGACCG TGGACCCCCT GGCCCTGTTG GCCCACCTGG TTTGACTGGA 
CCTGCTGGAG AGCCTGGACG TGAAGGTAAC GCTGGATCTG ATGGCCCACC TGGTCGTGAT 
GGTGCTACTG GAATTAAGGG TGATCGTGGT GAGACTGGTC CTCTTGGTGC TCCTGGTGCT 
CCCGGTGCCC CTGGTGCTCC TGGACCTGTT GGCCCAACTG GAAAACAAGG AGACAGAGGA 
GAATCTGGAC CCCAAGGTCC ACTTGGTCCA TCTGGTCCTG CTGGTGCTCG TGGCTTGCCT 
GGTCCTCAAG GTCCACGTGG TGATAAGGGT GAAGCTGGTG AGGCAGGAGA GAGAGGACAG 
AAAGGTCACA GAGGATTCAC TGGTCTTCAG GGTCTGCCAG GTCCCCCAGG TACTGCTGGT 
GACCAAGGTG CTTCTGGTCC TGCCGGTCCT GGTGGTCCTA GAGGTCCCCC TGGCCCTGTT 
GGTCCTTCTG GCAAAGATGG CTCTAATGGT CTTCCTGGTC CAATTGGTCC ACCTGGTCCA 
CGCGGTCGCG GTGGTGAAAC TGGTCCTGCT GGTCCACCTG GTCAACCTGG CCCACCTGGT 
CCCCCTGGTC CTCCTGGCCC TGGAATTGAC ATGTCTGCTT TCGCCGGCCT ATCTCAACCT 
GAAAAAGGAC CCGACCCAAT GCGTTACATG CGTGCTGACC AAGCCTCCAG CTCTGTCCCT 
CAGCGTGATG TTGATGTTGA AGCCACTTTG AAATCCCTTA ATAACCAGAT CGAAAGCATC 
CGCAGCCCAG ATGGTACCAA GAAGAACCCA GCCCGCACAT GCCGTGACCT GAAACTCTGC 
CATCCTGAAT GGAAGAGCGG TGACTATTGG ATCGATCCCA ACCAGGGCTG CACTGTAGAT 
GCTATCAAAG TCTTCTGCAA CATGGAGACT GGAGAGACCT GTGTATACCC CAACCCATCA 
AAGATCCCCA AGAAGAACTG GTGGTCCGCC AAGGGCAAGG AAAAGAAACA CATTTGGTTT 
GGAGAGACAA TCAATGGTGG CTTCCAATTT AGCTATGGAG ATGACAGCTC AGCACCCAAC 
ACTGCCAACA TTCAGATGAC CTTCCTGCGT CTGCTCTCCA CTGATGCAAC CCAGAACATC 
ACCTATCACT GCAAGAACAG CATTGCATTT ATGGATGAAG CTTCAGGCAA CCTTAAGAAG 
GCTGTTCTAC TCCAAGGATC CAATGATGTA GAAATCAGAG CTGAGGGCAA CAGTAGATTC 
ACATACAATG CCTTGGAAGA TGGCTGCAAG AAACACACTG GCAAATGGAG CAAGACAGTA 
ATTGAATACA GGACACAGAA AACATCCCGC CTGCCCATCG TAGACATTGC ACCTATGGAT 
ATTGGTGGCG CTGATCAGGA ATTTGGTGTT GACATTGGCC CAGTCTGCTT CTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col2a1 ( XM_012956663.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812117.1 Xenopus tropicalis collagen, type II, alpha 1 (col2a1), transcript variant X2, mRNA.

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CloneID	OXa26092
Clone ID Related Accession (Same CDS sequence)	XM_012956663.2
Accession Version	XM_012956663.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(II) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956663.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGCT TTGTGGATTC AAGGACTCTA GTGCTGTTCG CAGCCACACA AGTCATTTTA 
TTAGCGGTTG TACGATGCCA AGATGAAGAA GATGTCCGGC AAGGAGTACT CAAGGGCCAG 
AAAGGTGAAC CCGGTGATAT TAAAGATGTT GTAGGACCAA AAGGACCACC TGGACCACAG 
GGACCTTCTG GTGAACAAGG ACCTAGAGGA GATCGTGGAG ACAAGGGCGA GAAAGGTGCT 
CCTGGACCCC GTGGAAGAGA TGGCGAACCC GGTACTCCTG GGAACCCAGG ACCTGTTGGA 
CCCCCTGGAC CTCCTGGACC CCCTGGCCTT GGCGGAAACT TTGCTGCTCA AATGACAGGA 
GGATTTGATG AAAAAGCTGG TGGTGCACAA ATGGGAGTTA TGCAAGGACC AATGGGCCCT 
ATGGGACCAA GAGGACCTCC CGGCCCAACA GGAGCTCCTG GACCTCAAGG TTTCCAAGGC 
AATCCCGGTG AACCTGGTGA ACCTGGCGCT GGTGGTCCAA TGGGTCCCCG TGGTCCTCCT 
GGCCCTGCTG GAAAGCCTGG TGATGATGGT GAAGCTGGCA AACCTGGCAA ATCTGGTGAG 
CGTGGACCCC CAGGACCACA GGGAGCTAGA GGTTTCCCTG GAACCCCAGG ACTTCCAGGA 
GTAAAAGGAC ACAGGGGTTA CCCAGGTCTT GATGGGTCAA AGGGTGAAGC TGGTGCTGCT 
GGTGCCAAGG GAGAAGGTGG TGCTACTGGT GAAGCTGGAT CTCCTGGTCC AATGGGTCCC 
CGTGGTCTTC CAGGTGAGAG AGGACGCCCT GGAGCTTCTG GTGCTGCTGG TGCTCGTGGT 
AATGATGGTC TTCCTGGCCC TGCTGGTCCC CCAGGACCTG TTGGCCCTGC TGGTGCTCCT 
GGTTTCCCTG GTGCTCCTGG TTCAAAGGGT GAAGCTGGCC CAACTGGTGC TCGTGGACCT 
GAGGGTGCTC AAGGACCCAG AGGAGAATCT GGTACCCCTG GATCTCCTGG ACCTGCTGGA 
GCTTCTGGTA ACCCTGGTAC TGATGGTATT CCTGGCGCCA AAGGTTCATC TGGTGCTCCT 
GGTATTGCTG GTGCCCCTGG TTTCCCTGGA CCACGTGGTC CTCCAGGACC TCAGGGAGCT 
ACTGGTCCTC TTGGTCCCAA AGGCCAGACT GGTGACCCCG GTGTTGCAGG TTTCAAGGGT 
GAACATGGTC CCAAAGGTGA AATCGGGTCT GCAGGTCCTC AGGGTGCTCC TGGCCCAGCT 
GGTGAAGAAG GCAAGAGAGG AGCCCGTGGT GAACCTGGTG CTGCTGGACC CCTTGGTCCT 
CCTGGAGAGA GAGGTGCTCC TGGTAATCGT GGTTTCCCTG GTCAAGATGG TCTTGCTGGT 
CCTAAGGGTG CTCCTGGTGA ACGTGGTGTT CCAGGTCTTG GTGGACCAAA GGGTGCTAAT 
GGTGATCCTG GCCGTCCTGG CGAACCTGGT CTCCCTGGTG CTAGGGGTCT TACTGGCCGT 
CCTGGTGATG CTGGTCCTCA GGGAAAAGTT GGGCCCTCTG GTGCTTCTGG TGAAGATGGT 
CGTCCTGGAC CTCCTGGGCC ACAAGGTGCT CGTGGTCAGC CTGGTGTTAT GGGTTTCCCT 
GGGCCTAAAG GTGCCAATGG CGAACCTGGC AAAGCTGGTG AGAAAGGACT GCTTGGTGCT 
CCTGGTCTGA GGGGTCTGCC TGGAAAAGAT GGTGAAACTG GTGCTCAAGG TCCCAATGGT 
CCAGCTGGAC CTGCTGGTGA AAGAGGTGAA CAAGGACCTC CTGGCCCATC TGGCTTCCAG 
GGACTTCCTG GACCTCCCGG TTCTCCCGGA GAAGGTGGCA AACCAGGTGA TCAGGGTGTT 
CCCGGTGAAG CAGGTGCCCC TGGTCTTGTT GGCCCAAGAG GTGAGCGTGG TTTCCCAGGT 
GAACGTGGTT CTTCAGGTCC TCAAGGTCTT CAGGGTCCTC GTGGTCTTCC TGGAACTCCT 
GGTACTGATG GTCCCAAGGG TGCAACTGGT CCATCTGGTC CCAATGGTGC CCAAGGTCCT 
CCTGGTCTTC AAGGTATGCC TGGTGAGAGA GGAGCTGCTG GTATTTCTGG ACCCAAGGGT 
GATAGAGGTG ATACTGGTGA GAAAGGCCCA GAGGGTGCTC CTGGTAAAGA TGGTTCAAGA 
GGTTTGACTG GTCCAATTGG TCCCCCTGGT CCATCTGGTC CTAATGGTGA GAAGGGAGAA 
TCTGGTCCTT CCGGTCCAGC TGGTATTGTT GGTGCCCGTG GTGCCCCTGG TGATCGTGGT 
GAGACTGGCC CTCCAGGTCC TGCTGGCTTT GCTGGTCCTC CTGGTGCTGA TGGTCAAGCT 
GGTCTTAAGG GTGATCAAGG TGAATCTGGC CAGAAGGGTG ATGCTGGTGC TCCTGGTCCC 
CAGGGTCCAT CTGGTGCTCC TGGCCCACAG GGCCCAACTG GTGTTAATGG TCCTAAGGGA 
GCTCGCGGTG CTCAAGGTCC TCCTGGTGCT ACTGGATTCC CTGGTGCTGC TGGAAGAGTT 
GGTCCCCCTG GTCCCAATGG TAACCCTGGA CCTTCAGGTG CTCCTGGATC TGCTGGTAAA 
GAAGGGCCAA AGGGTGCACG TGGTGATGCT GGTCCTACTG GTCGTGCTGG TGATCCAGGT 
CTTCAAGGTC CTGCTGGTGT TCCTGGAGAG AAGGGAGAGT CTGGTGAAGA TGGTCCTTCT 
GGTCCAGATG GTCCCCCAGG TCCTCAAGGT TTGTCTGGAC AGCGTGGTAT TGTTGGTCTG 
CCAGGTCAGC GTGGTGAAAG AGGATTCCCT GGACTTCCTG GTCCATCTGG TGAACCTGGA 
AAACAAGGAG GCCCTGGATC TGCTGGAGAC CGTGGACCCC CTGGCCCTGT TGGCCCACCT 
GGTTTGACTG GACCTGCTGG AGAGCCTGGA CGTGAAGGTA ACGCTGGATC TGATGGCCCA 
CCTGGTCGTG ATGGTGCTAC TGGAATTAAG GGTGATCGTG GTGAGACTGG TCCTCTTGGT 
GCTCCTGGTG CTCCCGGTGC CCCTGGTGCT CCTGGACCTG TTGGCCCAAC TGGAAAACAA 
GGAGACAGAG GAGAATCTGG ACCCCAAGGT CCACTTGGTC CATCTGGTCC TGCTGGTGCT 
CGTGGCTTGC CTGGTCCTCA AGGTCCACGT GGTGATAAGG GTGAAGCTGG TGAGGCAGGA 
GAGAGAGGAC AGAAAGGTCA CAGAGGATTC ACTGGTCTTC AGGGTCTGCC AGGTCCCCCA 
GGTACTGCTG GTGACCAAGG TGCTTCTGGT CCTGCCGGTC CTGGTGGTCC TAGAGGTCCC 
CCTGGCCCTG TTGGTCCTTC TGGCAAAGAT GGCTCTAATG GTCTTCCTGG TCCAATTGGT 
CCACCTGGTC CACGCGGTCG CGGTGGTGAA ACTGGTCCTG CTGGTCCACC TGGTCAACCT 
GGCCCACCTG GTCCCCCTGG TCCTCCTGGC CCTGGAATTG ACATGTCTGC TTTCGCCGGC 
CTATCTCAAC CTGAAAAAGG ACCCGACCCA ATGCGTTACA TGCGTGCTGA CCAAGCCTCC 
AGCTCTGTCC CTCAGCGTGA TGTTGATGTT GAAGCCACTT TGAAATCCCT TAATAACCAG 
ATCGAAAGCA TCCGCAGCCC AGATGGTACC AAGAAGAACC CAGCCCGCAC ATGCCGTGAC 
CTGAAACTCT GCCATCCTGA ATGGAAGAGC GGTGACTATT GGATCGATCC CAACCAGGGC 
TGCACTGTAG ATGCTATCAA AGTCTTCTGC AACATGGAGA CTGGAGAGAC CTGTGTATAC 
CCCAACCCAT CAAAGATCCC CAAGAAGAAC TGGTGGTCCG CCAAGGGCAA GGAAAAGAAA 
CACATTTGGT TTGGAGAGAC AATCAATGGT GGCTTCCAAT TTAGCTATGG AGATGACAGC 
TCAGCACCCA ACACTGCCAA CATTCAGATG ACCTTCCTGC GTCTGCTCTC CACTGATGCA 
ACCCAGAACA TCACCTATCA CTGCAAGAAC AGCATTGCAT TTATGGATGA AGCTTCAGGC 
AACCTTAAGA AGGCTGTTCT ACTCCAAGGA TCCAATGATG TAGAAATCAG AGCTGAGGGC 
AACAGTAGAT TCACATACAA TGCCTTGGAA GATGGCTGCA AGAAACACAC TGGCAAATGG 
AGCAAGACAG TAATTGAATA CAGGACACAG AAAACATCCC GCCTGCCCAT CGTAGACATT 
GCACCTATGG ATATTGGTGG CGCTGATCAG GAATTTGGTG TTGACATTGG CCCAGTCTGC 
TTCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812117.1
CDS	205..4473
Translation	MFSFVDSRTLVLFAATQVILLAVVRCQDEEDVRQGVLKGQKGEPGDIKDVVGPKGPPGPQGPSGEQGPRGDRGDKGEKGAPGPRGRDGEPGTPGNPGPVGPPGPPGPPGLGGNFAAQMTGGFDEKAGGAQMGVMQGPMGPMGPRGPPGPTGAPGPQGFQGNPGEPGEPGAGGPMGPRGPPGPAGKPGDDGEAGKPGKSGERGPPGPQGARGFPGTPGLPGVKGHRGYPGLDGSKGEAGAAGAKGEGGATGEAGSPGPMGPRGLPGERGRPGASGAAGARGNDGLPGPAGPPGPVGPAGAPGFPGAPGSKGEAGPTGARGPEGAQGPRGESGTPGSPGPAGASGNPGTDGIPGAKGSSGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQTGDPGVAGFKGEHGPKGEIGSAGPQGAPGPAGEEGKRGARGEPGAAGPLGPPGERGAPGNRGFPGQDGLAGPKGAPGERGVPGLGGPKGANGDPGRPGEPGLPGARGLTGRPGDAGPQGKVGPSGASGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLLGAPGLRGLPGKDGETGAQGPNGPAGPAGERGEQGPPGPSGFQGLPGPPGSPGEGGKPGDQGVPGEAGAPGLVGPRGERGFPGERGSSGPQGLQGPRGLPGTPGTDGPKGATGPSGPNGAQGPPGLQGMPGERGAAGISGPKGDRGDTGEKGPEGAPGKDGSRGLTGPIGPPGPSGPNGEKGESGPSGPAGIVGARGAPGDRGETGPPGPAGFAGPPGADGQAGLKGDQGESGQKGDAGAPGPQGPSGAPGPQGPTGVNGPKGARGAQGPPGATGFPGAAGRVGPPGPNGNPGPSGAPGSAGKEGPKGARGDAGPTGRAGDPGLQGPAGVPGEKGESGEDGPSGPDGPPGPQGLSGQRGIVGLPGQRGERGFPGLPGPSGEPGKQGGPGSAGDRGPPGPVGPPGLTGPAGEPGREGNAGSDGPPGRDGATGIKGDRGETGPLGAPGAPGAPGAPGPVGPTGKQGDRGESGPQGPLGPSGPAGARGLPGPQGPRGDKGEAGEAGERGQKGHRGFTGLQGLPGPPGTAGDQGASGPAGPGGPRGPPGPVGPSGKDGSNGLPGPIGPPGPRGRGGETGPAGPPGQPGPPGPPGPPGPGIDMSAFAGLSQPEKGPDPMRYMRADQASSSVPQRDVDVEATLKSLNNQIESIRSPDGTKKNPARTCRDLKLCHPEWKSGDYWIDPNQGCTVDAIKVFCNMETGETCVYPNPSKIPKKNWWSAKGKEKKHIWFGETINGGFQFSYGDDSSAPNTANIQMTFLRLLSTDATQNITYHCKNSIAFMDEASGNLKKAVLLQGSNDVEIRAEGNSRFTYNALEDGCKKHTGKWSKTVIEYRTQKTSRLPIVDIAPMDIGGADQEFGVDIGPVCFL

Target ORF information:

RefSeq Version	XM_012956663.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type II, alpha 1 (col2a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956663.2

ORF Insert Sequence:

ATGTTTAGCT TTGTGGATTC AAGGACTCTA GTGCTGTTCG CAGCCACACA AGTCATTTTA 
TTAGCGGTTG TACGATGCCA AGATGAAGAA GATGTCCGGC AAGGAGTACT CAAGGGCCAG 
AAAGGTGAAC CCGGTGATAT TAAAGATGTT GTAGGACCAA AAGGACCACC TGGACCACAG 
GGACCTTCTG GTGAACAAGG ACCTAGAGGA GATCGTGGAG ACAAGGGCGA GAAAGGTGCT 
CCTGGACCCC GTGGAAGAGA TGGCGAACCC GGTACTCCTG GGAACCCAGG ACCTGTTGGA 
CCCCCTGGAC CTCCTGGACC CCCTGGCCTT GGCGGAAACT TTGCTGCTCA AATGACAGGA 
GGATTTGATG AAAAAGCTGG TGGTGCACAA ATGGGAGTTA TGCAAGGACC AATGGGCCCT 
ATGGGACCAA GAGGACCTCC CGGCCCAACA GGAGCTCCTG GACCTCAAGG TTTCCAAGGC 
AATCCCGGTG AACCTGGTGA ACCTGGCGCT GGTGGTCCAA TGGGTCCCCG TGGTCCTCCT 
GGCCCTGCTG GAAAGCCTGG TGATGATGGT GAAGCTGGCA AACCTGGCAA ATCTGGTGAG 
CGTGGACCCC CAGGACCACA GGGAGCTAGA GGTTTCCCTG GAACCCCAGG ACTTCCAGGA 
GTAAAAGGAC ACAGGGGTTA CCCAGGTCTT GATGGGTCAA AGGGTGAAGC TGGTGCTGCT 
GGTGCCAAGG GAGAAGGTGG TGCTACTGGT GAAGCTGGAT CTCCTGGTCC AATGGGTCCC 
CGTGGTCTTC CAGGTGAGAG AGGACGCCCT GGAGCTTCTG GTGCTGCTGG TGCTCGTGGT 
AATGATGGTC TTCCTGGCCC TGCTGGTCCC CCAGGACCTG TTGGCCCTGC TGGTGCTCCT 
GGTTTCCCTG GTGCTCCTGG TTCAAAGGGT GAAGCTGGCC CAACTGGTGC TCGTGGACCT 
GAGGGTGCTC AAGGACCCAG AGGAGAATCT GGTACCCCTG GATCTCCTGG ACCTGCTGGA 
GCTTCTGGTA ACCCTGGTAC TGATGGTATT CCTGGCGCCA AAGGTTCATC TGGTGCTCCT 
GGTATTGCTG GTGCCCCTGG TTTCCCTGGA CCACGTGGTC CTCCAGGACC TCAGGGAGCT 
ACTGGTCCTC TTGGTCCCAA AGGCCAGACT GGTGACCCCG GTGTTGCAGG TTTCAAGGGT 
GAACATGGTC CCAAAGGTGA AATCGGGTCT GCAGGTCCTC AGGGTGCTCC TGGCCCAGCT 
GGTGAAGAAG GCAAGAGAGG AGCCCGTGGT GAACCTGGTG CTGCTGGACC CCTTGGTCCT 
CCTGGAGAGA GAGGTGCTCC TGGTAATCGT GGTTTCCCTG GTCAAGATGG TCTTGCTGGT 
CCTAAGGGTG CTCCTGGTGA ACGTGGTGTT CCAGGTCTTG GTGGACCAAA GGGTGCTAAT 
GGTGATCCTG GCCGTCCTGG CGAACCTGGT CTCCCTGGTG CTAGGGGTCT TACTGGCCGT 
CCTGGTGATG CTGGTCCTCA GGGAAAAGTT GGGCCCTCTG GTGCTTCTGG TGAAGATGGT 
CGTCCTGGAC CTCCTGGGCC ACAAGGTGCT CGTGGTCAGC CTGGTGTTAT GGGTTTCCCT 
GGGCCTAAAG GTGCCAATGG CGAACCTGGC AAAGCTGGTG AGAAAGGACT GCTTGGTGCT 
CCTGGTCTGA GGGGTCTGCC TGGAAAAGAT GGTGAAACTG GTGCTCAAGG TCCCAATGGT 
CCAGCTGGAC CTGCTGGTGA AAGAGGTGAA CAAGGACCTC CTGGCCCATC TGGCTTCCAG 
GGACTTCCTG GACCTCCCGG TTCTCCCGGA GAAGGTGGCA AACCAGGTGA TCAGGGTGTT 
CCCGGTGAAG CAGGTGCCCC TGGTCTTGTT GGCCCAAGAG GTGAGCGTGG TTTCCCAGGT 
GAACGTGGTT CTTCAGGTCC TCAAGGTCTT CAGGGTCCTC GTGGTCTTCC TGGAACTCCT 
GGTACTGATG GTCCCAAGGG TGCAACTGGT CCATCTGGTC CCAATGGTGC CCAAGGTCCT 
CCTGGTCTTC AAGGTATGCC TGGTGAGAGA GGAGCTGCTG GTATTTCTGG ACCCAAGGGT 
GATAGAGGTG ATACTGGTGA GAAAGGCCCA GAGGGTGCTC CTGGTAAAGA TGGTTCAAGA 
GGTTTGACTG GTCCAATTGG TCCCCCTGGT CCATCTGGTC CTAATGGTGA GAAGGGAGAA 
TCTGGTCCTT CCGGTCCAGC TGGTATTGTT GGTGCCCGTG GTGCCCCTGG TGATCGTGGT 
GAGACTGGCC CTCCAGGTCC TGCTGGCTTT GCTGGTCCTC CTGGTGCTGA TGGTCAAGCT 
GGTCTTAAGG GTGATCAAGG TGAATCTGGC CAGAAGGGTG ATGCTGGTGC TCCTGGTCCC 
CAGGGTCCAT CTGGTGCTCC TGGCCCACAG GGCCCAACTG GTGTTAATGG TCCTAAGGGA 
GCTCGCGGTG CTCAAGGTCC TCCTGGTGCT ACTGGATTCC CTGGTGCTGC TGGAAGAGTT 
GGTCCCCCTG GTCCCAATGG TAACCCTGGA CCTTCAGGTG CTCCTGGATC TGCTGGTAAA 
GAAGGGCCAA AGGGTGCACG TGGTGATGCT GGTCCTACTG GTCGTGCTGG TGATCCAGGT 
CTTCAAGGTC CTGCTGGTGT TCCTGGAGAG AAGGGAGAGT CTGGTGAAGA TGGTCCTTCT 
GGTCCAGATG GTCCCCCAGG TCCTCAAGGT TTGTCTGGAC AGCGTGGTAT TGTTGGTCTG 
CCAGGTCAGC GTGGTGAAAG AGGATTCCCT GGACTTCCTG GTCCATCTGG TGAACCTGGA 
AAACAAGGAG GCCCTGGATC TGCTGGAGAC CGTGGACCCC CTGGCCCTGT TGGCCCACCT 
GGTTTGACTG GACCTGCTGG AGAGCCTGGA CGTGAAGGTA ACGCTGGATC TGATGGCCCA 
CCTGGTCGTG ATGGTGCTAC TGGAATTAAG GGTGATCGTG GTGAGACTGG TCCTCTTGGT 
GCTCCTGGTG CTCCCGGTGC CCCTGGTGCT CCTGGACCTG TTGGCCCAAC TGGAAAACAA 
GGAGACAGAG GAGAATCTGG ACCCCAAGGT CCACTTGGTC CATCTGGTCC TGCTGGTGCT 
CGTGGCTTGC CTGGTCCTCA AGGTCCACGT GGTGATAAGG GTGAAGCTGG TGAGGCAGGA 
GAGAGAGGAC AGAAAGGTCA CAGAGGATTC ACTGGTCTTC AGGGTCTGCC AGGTCCCCCA 
GGTACTGCTG GTGACCAAGG TGCTTCTGGT CCTGCCGGTC CTGGTGGTCC TAGAGGTCCC 
CCTGGCCCTG TTGGTCCTTC TGGCAAAGAT GGCTCTAATG GTCTTCCTGG TCCAATTGGT 
CCACCTGGTC CACGCGGTCG CGGTGGTGAA ACTGGTCCTG CTGGTCCACC TGGTCAACCT 
GGCCCACCTG GTCCCCCTGG TCCTCCTGGC CCTGGAATTG ACATGTCTGC TTTCGCCGGC 
CTATCTCAAC CTGAAAAAGG ACCCGACCCA ATGCGTTACA TGCGTGCTGA CCAAGCCTCC 
AGCTCTGTCC CTCAGCGTGA TGTTGATGTT GAAGCCACTT TGAAATCCCT TAATAACCAG 
ATCGAAAGCA TCCGCAGCCC AGATGGTACC AAGAAGAACC CAGCCCGCAC ATGCCGTGAC 
CTGAAACTCT GCCATCCTGA ATGGAAGAGC GGTGACTATT GGATCGATCC CAACCAGGGC 
TGCACTGTAG ATGCTATCAA AGTCTTCTGC AACATGGAGA CTGGAGAGAC CTGTGTATAC 
CCCAACCCAT CAAAGATCCC CAAGAAGAAC TGGTGGTCCG CCAAGGGCAA GGAAAAGAAA 
CACATTTGGT TTGGAGAGAC AATCAATGGT GGCTTCCAAT TTAGCTATGG AGATGACAGC 
TCAGCACCCA ACACTGCCAA CATTCAGATG ACCTTCCTGC GTCTGCTCTC CACTGATGCA 
ACCCAGAACA TCACCTATCA CTGCAAGAAC AGCATTGCAT TTATGGATGA AGCTTCAGGC 
AACCTTAAGA AGGCTGTTCT ACTCCAAGGA TCCAATGATG TAGAAATCAG AGCTGAGGGC 
AACAGTAGAT TCACATACAA TGCCTTGGAA GATGGCTGCA AGAAACACAC TGGCAAATGG 
AGCAAGACAG TAATTGAATA CAGGACACAG AAAACATCCC GCCTGCCCAT CGTAGACATT 
GCACCTATGG ATATTGGTGG CGCTGATCAG GAATTTGGTG TTGACATTGG CCCAGTCTGC 
TTCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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col2a1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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