LOC101731929 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following LOC101731929 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101731929 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa57030 XM_004912313.3
Latest version!
Xenopus tropicalis uncharacterized LOC101731929 (LOC101731929), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa06664 XM_004912313.2
Latest version!
Xenopus tropicalis uncharacterized LOC101731929 (LOC101731929), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa57030
    Clone ID Related Accession (Same CDS sequence) XM_004912313.3
    Accession Version XM_004912313.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4473bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product uncharacterized protein LOC101731929
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912313.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTGTTA GCCAAGCATC ATCATACAAA ACCAGATCAC GAGCAAGCTG CTCTAGCAAG 
    TCCTCCATAA AAGAAATGGC TGCTCTCGCA CGTGCAGAAG CTGAAGCTGC AAAAGTAAAG
    TCTTCCTTTG CTGCACAAGA GATGCAACTA AAGAAAGAGA GGGCATCACT AGAAAAAGAA
    CGAGTAGATC TGCAAACATC GCTAGAAAGA CTCGCCGCAG AGAAAGAAGC GGCAGCCGCA
    ATAGCCAAAG CTGAATACCT GGAAGCCCTG GAGTTTTCCG GATCCGAGAA ACACAGCCAA
    GTACTTGGGC CGGACCTAGA CGAACAAGAT CCAGCCCAGC GTGTCTCAGA GTACGTCCAC
    CAACATCCCA AGAAAGAGGA CAACTATGAG CCAGCACATC CGCCAGCTGA CACAGAGTGC
    CAAAGATCCT ACCCCCAACC ACAGTACCTT AGGCAGGACG ATATGCGACC AAACGATGCT
    GACAACTACT GCCCTACGGA GCAGAGACTA CGACCTTTGC CTCGAATGAA AGGAACACCT
    TTTGCATCAG AACGGTATTA TACTGACTGC TCTAAACAAG AAGCTCTTAA AAGGTACGGT
    AATACACCAC ACATGCAGCA TCATAGCACA CCAGGTAGTA CTGATGCTAA CTGGGCCATC
    ATGGACTTTG CGAAGTTCTT TGCTAAACGG GAGCTGGTTA CCAGAGGACT TACAAAGTTC
    ACCGACCAGG CCGAGAACTA CAGGTCATGG CGAGCCTCCT TCCAAAACAC CATAAGAGAC
    TTAGACCTTT CCCATCAGGA AGAGTTAGAC CTCCTGGTAA AGTGGCTTGG AAGCGAGTCT
    GTTGAACACG CCAAAAGAAT CAGAGACATT AACATAAACT ATCCTGGCAG AGGCCTAAAA
    ATGGTGTGGG AAAGACTTAA TGAGTGTTAT GGGTCCATAG AAGCCATTGA AAATGCTTTG
    TTCAAAAGGA TTGATAACTT TCCCAGAATA GTGGGTAAAG GTTACCAGAA GCTCAGGGAG
    TTATGTGACT TGTTAAGAGA GGTACAGGTT GCTCAAGCAG AAGGAGATCT ACCAGGGTTA
    GCATTCCTAG ACACTGCCAG AGGGGTTAAT CCGATTGTTC AAAAACTCCC TTATAACTTA
    CAAGAGAAGT GGGTCACACA TGGTTCTACT TATAAACGGG TTCATAATGT ACCATTTCCC
    CCCTTCAAGG TGTTTGTAGA CTTTGTCAGT GAACAGGCAA GAATCAGAAA TGATCCCAGT
    TTTGATCTCA CAATGTCATG TCCCACTGCC CCTGGTGTCA GACCTCATAA AACACCAGTG
    GCAGTCCACA AAACTAACAT TGCCTCCACA GGTTCTCCAT ACAAACCATC TAAGTCTTCT
    CAAGAAGAGA ACAGACACAG AGATCTTAAC CAAGAATGCC CCCTGCACAA GAAACCACAT
    TCTTTACTAA AATGCAGAGC CTTCAGAGAA AAGACTTTAG AGGACCGCAA AGAGTTCCTT
    AAAGACAACC ACATCTGCTT CAGGTGCTGC ACTACAACAT CGCACCTAGC AAGGAACTGT
    GAGGTCAGTG TGTCATGCGC AGAATGTGGT AGTAAAGAAC ACAATACGGC TTTACACCCT
    GGGCCACCTT CTCAGGCATT ACACCAAACA GAGGACCATC AAAAGAAGCA GATAAACACT
    GACAAAACCA ACACAGTAGT CACTTCTCAG TGTACCGAGA TCTGTAAAGG AGTGGCAGGA
    GGAAAGTCCT GCTCAAAAAT CTGCCTTGTC AGAGTTTACC CGGCCAGTCA CCGAGATAAA
    GCGATTAAGG TATACGCAAT CCTAGACGAT CAAAGCAACC GGTCTTTAGC AAAATCCACC
    CTTTTTGACA CCTTCAACAT CATAGGACCC GGTTCTCCAT ACTCCTTAAA GACATGTGCA
    GGTACCGTTG AGACGGCGGG GAGGAAGGCA ACTGGATACA AGGTAGAGTC CATAGACGGG
    CAGACCTGCT TGTCACTGCC AACCATACTG GAGTGCAACC AAATTCCCGA CAACCGTTCT
    GAGATACCTA CACCAGAAGT AGCAGCATAC CACCCCCACC TGAGGCGTAT AGCTCACCAG
    ATACCAGAAC TGGATCCAGA AGCACCAATA GCCTTACTCC TAGGAAGAGA CATACTACGT
    GTTCACAAAG CTAGAGGACA GATTAATGGT TCCCAAAACG CTCCATACGC CCAGAGACTT
    GACCTAGGGT GGGTCATCAT AGGAGATGTC TGTCTAGGAG GAGTGCACAA GCCAATCTCT
    GTCAACAGCA TGCTCACCAG CACGGTTGAA AATGGACGTC CCTCCTTATT CCAACCATGT
    CAGAACAGTT TCCTGATAAA AGAATTGCCA CACAGAGATC CTGTCCCTTG TCCCTTCATG
    GATCCTTCTA GCGAAGACCA CGCCTGCGAG GGTGAACGAG ATCACTTAGG GTGTACAGTT
    TTCCATAGGT CAAGAGAAGA CAACAAGGTC GCAATGTCCA TAGAAGACAG GCTGTTCTTA
    GAGATCATGG ATCAAGGAAT GACAAAGGAC AAGTCAAGAA GCTGGGTCGC ACCTTTACCA
    TTTAGACCCC GAAGACAACG CTTACCTGAC AATAAAGAAC TTGTCTACAA AAGATTCATC
    TCCCTTAAGC ACAAACTTCA GAGGACACCA GAGATGAAAG AACATTTCTT TAACTTTATG
    CAAAGAATAT TCCAGAACAA CCATGCAGAA ATAGCACCTG CGCTCCGAGA CTCAGAGGAA
    CGTTGGTACT TACCCACATT CGGCGTATAT CATCCTAAAA AGCCAGGCCA GATACGAGTA
    GTGTTCGACT CAAGTGCCAG GTGCGAAGGT ATATCATTAA ACGATGTCTT ACTGTCTGGT
    CCAGACCTCA ACAACAGACT TCTAGAGGTA CTTCTACGTT TCCGTAAAGA TTCCATAGCA
    TTCATGGCCG ACGTACAACA GATGTTCTAC TGCTTCCTCG TCAAGGAGGA GCACAGAAAT
    TACTTGAGGT TCTTCTGGTA CCGCAACAAT GACCCTAATG AAGACATCGT AGAGTACCGT
    ATGAGGGTGC ACATCTTCGG AAACAGTCCT TCACCTGCAG TCGCTACCTA TGGTCTCAGA
    CTTTCAGCTC AAGAGGGTGA AGCAAAGTAT GGGTCAGATG TCAGATCCTT TGTAGAGAAA
    GATTTCTATG TGGATGACTG CTTGAAATCC ACACCTACGA ATGAGTCGGC AATCAGTCTC
    TTAAAAAGGA CTCAAGAGAT GCTCGCTTTA TCTAATCTGA GGTTACACAA AATTGCCTCC
    AACAGCCGTG AGTTAATGGA AGCCTTCTCA ACCCAAGACC ATTCGAGTGA CCTTAAGGAC
    TTGGATCTTG ACTCCGATTC CCTTCCCATG CAGCGGAGTC TTGGTTTGCT CTGGGACTTG
    AAGGCAGACA CCTTTACCTT CCAGATCAGC AAAGAAGAAA AGCCCTTCAC ACGCAGAGGA
    GTTCTGTCCA CGATAAACAG CTTGTACGAT CCTCTGGGAT TTGTAACACC TGTCACCATT
    CAAGGCAAAA TTATGTTAAG GGACCTCACC ACAGAGATGT CTGATTGGGA TGATCCGCTC
    CCCACAGAGA AAAAAGACCT ATGGACAGTT TGGAAAGGGT CTCTTGAAGC TTTGACCAGT
    CTTCATGTGA CACGACCCTA TGCTTCTATA CCATCTACAG AAGTCAAGAG ACAAAAGCTA
    CATATCTTCT GCGATGCTTC AATCAAGGCA ATCGCTGCTG TAGCATACCT AAAGACCATT
    GATGACAAAG AACAATGCCA TGTAGGATTC GTCATGAGTC GGACCAAACT GGCACCTCTC
    CGCGAACACA CAATACCCAG ACTGGAGCTC TGTGCTGCTG TGCTTGCAGT GGAGTTGGCT
    GAACTTATAA CTTCAGGAAT AGGCCTAGAA ATCAAAGAAG TCGAGTTCCA CACGGACAGT
    AAGGTAGTCC TAGGATATAT CTGCAACGAA ACTAGACGCT TTTATGTCTA CGTTTCCAAT
    CGGGTGCTGA GGATCAGGAG ATCCACTAGT CCTCAACAGT GGCACTACGT GCCTACACAA
    CATAATCCTG CAGACCACGC AACCAGATCC ATCGCAGCCT GCCACCTGAA AGCCACAACA
    TGGTTCACAG GTCCTGCGTT CCTGTACCGT TCAACTGCTT GCGATATCGG ATACGACACA
    TTTGAATTGA TAGACCCAGA CGCGGATGAA GAGATCCGAC CTGAAGTGTC TGTCCTTAAC
    ACAGTGACTT CTGATCATCA ACTTGAATCT CACCGTTTCA GCAGGTTCTC CACCTGGATG
    TCTCTGGTCC GTGCGATAGC CATCTTGATT CATATAGCTA AGTCCTACAC ATCTACATTA
    ACTGTGAATC AGAAACCTTG TAAAGGTTGG CATCACTGCG AGAACACCTT TACTGCTTTC
    AATCTGGAGA AGACAAGTAG TGACGTCCGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004912370.2
    CDS715..5187
    Translation

    Target ORF information:

    RefSeq Version XM_004912313.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis uncharacterized LOC101731929 (LOC101731929), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004912313.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTGTTA GCCAAGCATC ATCATACAAA ACCAGATCAC GAGCAAGCTG CTCTAGCAAG 
    TCCTCCATAA AAGAAATGGC TGCTCTCGCA CGTGCAGAAG CTGAAGCTGC AAAAGTAAAG
    TCTTCCTTTG CTGCACAAGA GATGCAACTA AAGAAAGAGA GGGCATCACT AGAAAAAGAA
    CGAGTAGATC TGCAAACATC GCTAGAAAGA CTCGCCGCAG AGAAAGAAGC GGCAGCCGCA
    ATAGCCAAAG CTGAATACCT GGAAGCCCTG GAGTTTTCCG GATCCGAGAA ACACAGCCAA
    GTACTTGGGC CGGACCTAGA CGAACAAGAT CCAGCCCAGC GTGTCTCAGA GTACGTCCAC
    CAACATCCCA AGAAAGAGGA CAACTATGAG CCAGCACATC CGCCAGCTGA CACAGAGTGC
    CAAAGATCCT ACCCCCAACC ACAGTACCTT AGGCAGGACG ATATGCGACC AAACGATGCT
    GACAACTACT GCCCTACGGA GCAGAGACTA CGACCTTTGC CTCGAATGAA AGGAACACCT
    TTTGCATCAG AACGGTATTA TACTGACTGC TCTAAACAAG AAGCTCTTAA AAGGTACGGT
    AATACACCAC ACATGCAGCA TCATAGCACA CCAGGTAGTA CTGATGCTAA CTGGGCCATC
    ATGGACTTTG CGAAGTTCTT TGCTAAACGG GAGCTGGTTA CCAGAGGACT TACAAAGTTC
    ACCGACCAGG CCGAGAACTA CAGGTCATGG CGAGCCTCCT TCCAAAACAC CATAAGAGAC
    TTAGACCTTT CCCATCAGGA AGAGTTAGAC CTCCTGGTAA AGTGGCTTGG AAGCGAGTCT
    GTTGAACACG CCAAAAGAAT CAGAGACATT AACATAAACT ATCCTGGCAG AGGCCTAAAA
    ATGGTGTGGG AAAGACTTAA TGAGTGTTAT GGGTCCATAG AAGCCATTGA AAATGCTTTG
    TTCAAAAGGA TTGATAACTT TCCCAGAATA GTGGGTAAAG GTTACCAGAA GCTCAGGGAG
    TTATGTGACT TGTTAAGAGA GGTACAGGTT GCTCAAGCAG AAGGAGATCT ACCAGGGTTA
    GCATTCCTAG ACACTGCCAG AGGGGTTAAT CCGATTGTTC AAAAACTCCC TTATAACTTA
    CAAGAGAAGT GGGTCACACA TGGTTCTACT TATAAACGGG TTCATAATGT ACCATTTCCC
    CCCTTCAAGG TGTTTGTAGA CTTTGTCAGT GAACAGGCAA GAATCAGAAA TGATCCCAGT
    TTTGATCTCA CAATGTCATG TCCCACTGCC CCTGGTGTCA GACCTCATAA AACACCAGTG
    GCAGTCCACA AAACTAACAT TGCCTCCACA GGTTCTCCAT ACAAACCATC TAAGTCTTCT
    CAAGAAGAGA ACAGACACAG AGATCTTAAC CAAGAATGCC CCCTGCACAA GAAACCACAT
    TCTTTACTAA AATGCAGAGC CTTCAGAGAA AAGACTTTAG AGGACCGCAA AGAGTTCCTT
    AAAGACAACC ACATCTGCTT CAGGTGCTGC ACTACAACAT CGCACCTAGC AAGGAACTGT
    GAGGTCAGTG TGTCATGCGC AGAATGTGGT AGTAAAGAAC ACAATACGGC TTTACACCCT
    GGGCCACCTT CTCAGGCATT ACACCAAACA GAGGACCATC AAAAGAAGCA GATAAACACT
    GACAAAACCA ACACAGTAGT CACTTCTCAG TGTACCGAGA TCTGTAAAGG AGTGGCAGGA
    GGAAAGTCCT GCTCAAAAAT CTGCCTTGTC AGAGTTTACC CGGCCAGTCA CCGAGATAAA
    GCGATTAAGG TATACGCAAT CCTAGACGAT CAAAGCAACC GGTCTTTAGC AAAATCCACC
    CTTTTTGACA CCTTCAACAT CATAGGACCC GGTTCTCCAT ACTCCTTAAA GACATGTGCA
    GGTACCGTTG AGACGGCGGG GAGGAAGGCA ACTGGATACA AGGTAGAGTC CATAGACGGG
    CAGACCTGCT TGTCACTGCC AACCATACTG GAGTGCAACC AAATTCCCGA CAACCGTTCT
    GAGATACCTA CACCAGAAGT AGCAGCATAC CACCCCCACC TGAGGCGTAT AGCTCACCAG
    ATACCAGAAC TGGATCCAGA AGCACCAATA GCCTTACTCC TAGGAAGAGA CATACTACGT
    GTTCACAAAG CTAGAGGACA GATTAATGGT TCCCAAAACG CTCCATACGC CCAGAGACTT
    GACCTAGGGT GGGTCATCAT AGGAGATGTC TGTCTAGGAG GAGTGCACAA GCCAATCTCT
    GTCAACAGCA TGCTCACCAG CACGGTTGAA AATGGACGTC CCTCCTTATT CCAACCATGT
    CAGAACAGTT TCCTGATAAA AGAATTGCCA CACAGAGATC CTGTCCCTTG TCCCTTCATG
    GATCCTTCTA GCGAAGACCA CGCCTGCGAG GGTGAACGAG ATCACTTAGG GTGTACAGTT
    TTCCATAGGT CAAGAGAAGA CAACAAGGTC GCAATGTCCA TAGAAGACAG GCTGTTCTTA
    GAGATCATGG ATCAAGGAAT GACAAAGGAC AAGTCAAGAA GCTGGGTCGC ACCTTTACCA
    TTTAGACCCC GAAGACAACG CTTACCTGAC AATAAAGAAC TTGTCTACAA AAGATTCATC
    TCCCTTAAGC ACAAACTTCA GAGGACACCA GAGATGAAAG AACATTTCTT TAACTTTATG
    CAAAGAATAT TCCAGAACAA CCATGCAGAA ATAGCACCTG CGCTCCGAGA CTCAGAGGAA
    CGTTGGTACT TACCCACATT CGGCGTATAT CATCCTAAAA AGCCAGGCCA GATACGAGTA
    GTGTTCGACT CAAGTGCCAG GTGCGAAGGT ATATCATTAA ACGATGTCTT ACTGTCTGGT
    CCAGACCTCA ACAACAGACT TCTAGAGGTA CTTCTACGTT TCCGTAAAGA TTCCATAGCA
    TTCATGGCCG ACGTACAACA GATGTTCTAC TGCTTCCTCG TCAAGGAGGA GCACAGAAAT
    TACTTGAGGT TCTTCTGGTA CCGCAACAAT GACCCTAATG AAGACATCGT AGAGTACCGT
    ATGAGGGTGC ACATCTTCGG AAACAGTCCT TCACCTGCAG TCGCTACCTA TGGTCTCAGA
    CTTTCAGCTC AAGAGGGTGA AGCAAAGTAT GGGTCAGATG TCAGATCCTT TGTAGAGAAA
    GATTTCTATG TGGATGACTG CTTGAAATCC ACACCTACGA ATGAGTCGGC AATCAGTCTC
    TTAAAAAGGA CTCAAGAGAT GCTCGCTTTA TCTAATCTGA GGTTACACAA AATTGCCTCC
    AACAGCCGTG AGTTAATGGA AGCCTTCTCA ACCCAAGACC ATTCGAGTGA CCTTAAGGAC
    TTGGATCTTG ACTCCGATTC CCTTCCCATG CAGCGGAGTC TTGGTTTGCT CTGGGACTTG
    AAGGCAGACA CCTTTACCTT CCAGATCAGC AAAGAAGAAA AGCCCTTCAC ACGCAGAGGA
    GTTCTGTCCA CGATAAACAG CTTGTACGAT CCTCTGGGAT TTGTAACACC TGTCACCATT
    CAAGGCAAAA TTATGTTAAG GGACCTCACC ACAGAGATGT CTGATTGGGA TGATCCGCTC
    CCCACAGAGA AAAAAGACCT ATGGACAGTT TGGAAAGGGT CTCTTGAAGC TTTGACCAGT
    CTTCATGTGA CACGACCCTA TGCTTCTATA CCATCTACAG AAGTCAAGAG ACAAAAGCTA
    CATATCTTCT GCGATGCTTC AATCAAGGCA ATCGCTGCTG TAGCATACCT AAAGACCATT
    GATGACAAAG AACAATGCCA TGTAGGATTC GTCATGAGTC GGACCAAACT GGCACCTCTC
    CGCGAACACA CAATACCCAG ACTGGAGCTC TGTGCTGCTG TGCTTGCAGT GGAGTTGGCT
    GAACTTATAA CTTCAGGAAT AGGCCTAGAA ATCAAAGAAG TCGAGTTCCA CACGGACAGT
    AAGGTAGTCC TAGGATATAT CTGCAACGAA ACTAGACGCT TTTATGTCTA CGTTTCCAAT
    CGGGTGCTGA GGATCAGGAG ATCCACTAGT CCTCAACAGT GGCACTACGT GCCTACACAA
    CATAATCCTG CAGACCACGC AACCAGATCC ATCGCAGCCT GCCACCTGAA AGCCACAACA
    TGGTTCACAG GTCCTGCGTT CCTGTACCGT TCAACTGCTT GCGATATCGG ATACGACACA
    TTTGAATTGA TAGACCCAGA CGCGGATGAA GAGATCCGAC CTGAAGTGTC TGTCCTTAAC
    ACAGTGACTT CTGATCATCA ACTTGAATCT CACCGTTTCA GCAGGTTCTC CACCTGGATG
    TCTCTGGTCC GTGCGATAGC CATCTTGATT CATATAGCTA AGTCCTACAC ATCTACATTA
    ACTGTGAATC AGAAACCTTG TAAAGGTTGG CATCACTGCG AGAACACCTT TACTGCTTTC
    AATCTGGAGA AGACAAGTAG TGACGTCCGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06664
    Clone ID Related Accession (Same CDS sequence) XM_004912313.2
    Accession Version XM_004912313.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4473bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product uncharacterized protein LOC101731929
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683513.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_004912313.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTGTTA GCCAAGCATC ATCATACAAA ACCAGATCAC GAGCAAGCTG CTCTAGCAAG 
    TCCTCCATAA AAGAAATGGC TGCTCTCGCA CGTGCAGAAG CTGAAGCTGC AAAAGTAAAG
    TCTTCCTTTG CTGCACAAGA GATGCAACTA AAGAAAGAGA GGGCATCACT AGAAAAAGAA
    CGAGTAGATC TGCAAACATC GCTAGAAAGA CTCGCCGCAG AGAAAGAAGC GGCAGCCGCA
    ATAGCCAAAG CTGAATACCT GGAAGCCCTG GAGTTTTCCG GATCCGAGAA ACACAGCCAA
    GTACTTGGGC CGGACCTAGA CGAACAAGAT CCAGCCCAGC GTGTCTCAGA GTACGTCCAC
    CAACATCCCA AGAAAGAGGA CAACTATGAG CCAGCACATC CGCCAGCTGA CACAGAGTGC
    CAAAGATCCT ACCCCCAACC ACAGTACCTT AGGCAGGACG ATATGCGACC AAACGATGCT
    GACAACTACT GCCCTACGGA GCAGAGACTA CGACCTTTGC CTCGAATGAA AGGAACACCT
    TTTGCATCAG AACGGTATTA TACTGACTGC TCTAAACAAG AAGCTCTTAA AAGGTACGGT
    AATACACCAC ACATGCAGCA TCATAGCACA CCAGGTAGTA CTGATGCTAA CTGGGCCATC
    ATGGACTTTG CGAAGTTCTT TGCTAAACGG GAGCTGGTTA CCAGAGGACT TACAAAGTTC
    ACCGACCAGG CCGAGAACTA CAGGTCATGG CGAGCCTCCT TCCAAAACAC CATAAGAGAC
    TTAGACCTTT CCCATCAGGA AGAGTTAGAC CTCCTGGTAA AGTGGCTTGG AAGCGAGTCT
    GTTGAACACG CCAAAAGAAT CAGAGACATT AACATAAACT ATCCTGGCAG AGGCCTAAAA
    ATGGTGTGGG AAAGACTTAA TGAGTGTTAT GGGTCCATAG AAGCCATTGA AAATGCTTTG
    TTCAAAAGGA TTGATAACTT TCCCAGAATA GTGGGTAAAG GTTACCAGAA GCTCAGGGAG
    TTATGTGACT TGTTAAGAGA GGTACAGGTT GCTCAAGCAG AAGGAGATCT ACCAGGGTTA
    GCATTCCTAG ACACTGCCAG AGGGGTTAAT CCGATTGTTC AAAAACTCCC TTATAACTTA
    CAAGAGAAGT GGGTCACACA TGGTTCTACT TATAAACGGG TTCATAATGT ACCATTTCCC
    CCCTTCAAGG TGTTTGTAGA CTTTGTCAGT GAACAGGCAA GAATCAGAAA TGATCCCAGT
    TTTGATCTCA CAATGTCATG TCCCACTGCC CCTGGTGTCA GACCTCATAA AACACCAGTG
    GCAGTCCACA AAACTAACAT TGCCTCCACA GGTTCTCCAT ACAAACCATC TAAGTCTTCT
    CAAGAAGAGA ACAGACACAG AGATCTTAAC CAAGAATGCC CCCTGCACAA GAAACCACAT
    TCTTTACTAA AATGCAGAGC CTTCAGAGAA AAGACTTTAG AGGACCGCAA AGAGTTCCTT
    AAAGACAACC ACATCTGCTT CAGGTGCTGC ACTACAACAT CGCACCTAGC AAGGAACTGT
    GAGGTCAGTG TGTCATGCGC AGAATGTGGT AGTAAAGAAC ACAATACGGC TTTACACCCT
    GGGCCACCTT CTCAGGCATT ACACCAAACA GAGGACCATC AAAAGAAGCA GATAAACACT
    GACAAAACCA ACACAGTAGT CACTTCTCAG TGTACCGAGA TCTGTAAAGG AGTGGCAGGA
    GGAAAGTCCT GCTCAAAAAT CTGCCTTGTC AGAGTTTACC CGGCCAGTCA CCGAGATAAA
    GCGATTAAGG TATACGCAAT CCTAGACGAT CAAAGCAACC GGTCTTTAGC AAAATCCACC
    CTTTTTGACA CCTTCAACAT CATAGGACCC GGTTCTCCAT ACTCCTTAAA GACATGTGCA
    GGTACCGTTG AGACGGCGGG GAGGAAGGCA ACTGGATACA AGGTAGAGTC CATAGACGGG
    CAGACCTGCT TATCACTGCC AACCATACTG GAGTGCAACC AAATTCCCGA CAACCGTTCT
    GAGATACCTA CACCAGAAGT AGCAGCATAC CACCCCCACC TGAGGCGTAT AGCTCACCAG
    ATACCAGAAC TGGATCCAGA AGCACCAATA GCCTTACTCC TAGGAAGAGA CATACTACGT
    GTTCACAAAG CTAGAGGACA GATTAATGGT TCCCAAAACG CTCCATACGC CCAGAGACTT
    GACCTAGGGT GGGTCATCAT AGGAGATGTC TGTCTAGGAG GAGTGCACAA GCCAATCTCT
    GTCAACAGCA TGCTCACCAG CACAGTTGAA AATGGACGTC CCTCCTTATT CCAACCATGT
    CAGAACAGTT TCCTGATAAA AGAATTGCCA CACAGAGATC CTGTCCCTTG TCCCTTCATG
    GATCCTTCTA GTGAAGACCA CGCCTGCGAG GGTGAACAAG ATCACTTAGG GTGTACAGTT
    TTCCATAGGT CTAGAGAAGA CAACAAGGTC GCAATGTCCA TAGAAGACAG GCTGTTCTTA
    GAGATCATGG ATCAAGGAAT GACAAAGGAC AAGTCAAAAA GCTGGGTCGC ACCTTTACCA
    TTTAGACCCC GAAGACAACG CTTACCTGAC AATAAAGAAC TTGTCTACAA AAGATTCATC
    TCCCTTAAGC ACAAACTTCA GAGGACACCA GAGATGAAAG AACATTTCTT TAACTTTATG
    CAAAGAATAT TCCAGAACAA CCATGCAGAA ATAGCACCTG CGCTCCGAGA CTCAGAGGAA
    CGTTGGTACT TACCCACATT CGGCGTATAT CATCCTAAAA AGCCAGGCCA GATACGAGTA
    GTGTTCGACT CAAGTGCCAG GTGCGAAGGT ATATCATTAA ACGATGTCTT ACTGTCTGGT
    CCAGACCTCA ACAACAGACT TCTAGAGGTA CTTCTACGTT TCCGTAAAGA TTCCATAGCA
    TTCATGGCCG ACATACAACA GATGTTCTAC TGCTTCCTCG TCAAGGAGGA GCACAGAAAT
    TACTTGAGGT TCTTCTGGTA CCGCAGCAAT GACCCTAATG AAGACATCGT AGAGTACCGT
    ATGAGGGTGC ACATCTTCGG AAACAGTCCT TCACCTGCAG TCGCTACCTA TGGTCTCAGA
    CTTTCAGCTC AAGAGGGTGA AGCAAAGTAT GGGTCAGATG TCAGATCCTT TGTAGAGAAA
    GATTTCTATG TGGATGACTG CTTGAAATCC ACACCTACGA ATGAGTCGGC AATCAGTCTC
    TTAAAAAGGA CTCAAGAGAT GCTCACTTTA TCTAATCTGA GGTTACACAA AATTGCCTCC
    AACAGCCGTG AGTTAATGGA AGCCTTCTCA ACCCAAGACC ATTCGAGTGA CCTTAAGGAC
    TTGGATCTTG ACTCCGATTC CCTTCCCATG CAGCGGAGTC TTGGTTTGCT CTGGGACTTG
    AAGGCAGACA CCTTTACCTT CCAGATCAGC AAAGAAGAAA AGCCCTTCAC ACGCAGAGGA
    GTTCTGTCCA CGATAAACAG CTTGTACGAT CCTCTGGGAT TTGTAACACC TGTCACCATT
    CAAGGCAAAA TTATGTTAAG GAACCTCACC ACAGAGATGT CTGATTGGGA TGATCCGCTC
    CCCACAGAGA AAAAAGACCT ATGGACAGTT TGGAAAGGGT CTCTTGAAGC TTTGACCAGT
    CTTCATGTGA CACGACCCTA TGCTTCCATA CCATCTACAG AAGTCAAGAG ACAAAAGCTA
    CATATCTTCT GCGATGCTTC AATCAAGGCA ATCGCTGCTG TAGCATACCT AAAGACCATT
    GATGACAAAG AACAATGCCA TGTAGGATTC GTCATGAGTC GGACCAAACT GGCACCTCTC
    CGCGAACACA CAATACCCAG ACTGGAGCTC TGTGCTGCTG TGCTTGCAGT GGAGTTGGCT
    GAACTTATAA CTTCAGGAAT AGGCCTAGAA GTCGAAGAAG TCGAGTTCCA CACGGACAGT
    AAGGTAGTCC TAGGATATAT CAGCAACGAA ACTAGACGCT TTTATGTCTA CGTCTCCAAT
    CGGGTGCTGA GGATCAGGAG ATCCACTAGT CCTCAACAGT GGCACTACGT GCCTACACAA
    CATAATCCTG CAGACCACGC AACCAGATCC ATCACAGCCT GCCACCTGAA AGCCACAACA
    TGGTTCACAG GTCCTGCGTT CCTGTACCGT TCAACTGCTT GCGATATCGG ATACGACACA
    TTTGAATTGA TAGACCCAGA CGCGGATGAA GAGATCCGAC CTGAAGTGTC TGTTCTTAAC
    ACAGTGACTT CTGATCATCA ACTTGAATCT CACCATTTCA GCAGGTTCTC CACCTGGACG
    TCTCTGGTCC GTGCGATAGC CATCTTGATT CATATAGCTA AGTCCTACAC ATCTACATTA
    ACTGTGAATC AGAAACCTTG TAAAGGTTGG CATCACTGCG AGAACACCTT TACTGCTTTC
    AATCTGGAGA AGACAAGTAG TGACGTCCGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004912370.1
    CDS713..5185
    Translation

    Target ORF information:

    RefSeq Version XM_004912313.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis uncharacterized LOC101731929 (LOC101731929), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004912313.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTGTTA GCCAAGCATC ATCATACAAA ACCAGATCAC GAGCAAGCTG CTCTAGCAAG 
    TCCTCCATAA AAGAAATGGC TGCTCTCGCA CGTGCAGAAG CTGAAGCTGC AAAAGTAAAG
    TCTTCCTTTG CTGCACAAGA GATGCAACTA AAGAAAGAGA GGGCATCACT AGAAAAAGAA
    CGAGTAGATC TGCAAACATC GCTAGAAAGA CTCGCCGCAG AGAAAGAAGC GGCAGCCGCA
    ATAGCCAAAG CTGAATACCT GGAAGCCCTG GAGTTTTCCG GATCCGAGAA ACACAGCCAA
    GTACTTGGGC CGGACCTAGA CGAACAAGAT CCAGCCCAGC GTGTCTCAGA GTACGTCCAC
    CAACATCCCA AGAAAGAGGA CAACTATGAG CCAGCACATC CGCCAGCTGA CACAGAGTGC
    CAAAGATCCT ACCCCCAACC ACAGTACCTT AGGCAGGACG ATATGCGACC AAACGATGCT
    GACAACTACT GCCCTACGGA GCAGAGACTA CGACCTTTGC CTCGAATGAA AGGAACACCT
    TTTGCATCAG AACGGTATTA TACTGACTGC TCTAAACAAG AAGCTCTTAA AAGGTACGGT
    AATACACCAC ACATGCAGCA TCATAGCACA CCAGGTAGTA CTGATGCTAA CTGGGCCATC
    ATGGACTTTG CGAAGTTCTT TGCTAAACGG GAGCTGGTTA CCAGAGGACT TACAAAGTTC
    ACCGACCAGG CCGAGAACTA CAGGTCATGG CGAGCCTCCT TCCAAAACAC CATAAGAGAC
    TTAGACCTTT CCCATCAGGA AGAGTTAGAC CTCCTGGTAA AGTGGCTTGG AAGCGAGTCT
    GTTGAACACG CCAAAAGAAT CAGAGACATT AACATAAACT ATCCTGGCAG AGGCCTAAAA
    ATGGTGTGGG AAAGACTTAA TGAGTGTTAT GGGTCCATAG AAGCCATTGA AAATGCTTTG
    TTCAAAAGGA TTGATAACTT TCCCAGAATA GTGGGTAAAG GTTACCAGAA GCTCAGGGAG
    TTATGTGACT TGTTAAGAGA GGTACAGGTT GCTCAAGCAG AAGGAGATCT ACCAGGGTTA
    GCATTCCTAG ACACTGCCAG AGGGGTTAAT CCGATTGTTC AAAAACTCCC TTATAACTTA
    CAAGAGAAGT GGGTCACACA TGGTTCTACT TATAAACGGG TTCATAATGT ACCATTTCCC
    CCCTTCAAGG TGTTTGTAGA CTTTGTCAGT GAACAGGCAA GAATCAGAAA TGATCCCAGT
    TTTGATCTCA CAATGTCATG TCCCACTGCC CCTGGTGTCA GACCTCATAA AACACCAGTG
    GCAGTCCACA AAACTAACAT TGCCTCCACA GGTTCTCCAT ACAAACCATC TAAGTCTTCT
    CAAGAAGAGA ACAGACACAG AGATCTTAAC CAAGAATGCC CCCTGCACAA GAAACCACAT
    TCTTTACTAA AATGCAGAGC CTTCAGAGAA AAGACTTTAG AGGACCGCAA AGAGTTCCTT
    AAAGACAACC ACATCTGCTT CAGGTGCTGC ACTACAACAT CGCACCTAGC AAGGAACTGT
    GAGGTCAGTG TGTCATGCGC AGAATGTGGT AGTAAAGAAC ACAATACGGC TTTACACCCT
    GGGCCACCTT CTCAGGCATT ACACCAAACA GAGGACCATC AAAAGAAGCA GATAAACACT
    GACAAAACCA ACACAGTAGT CACTTCTCAG TGTACCGAGA TCTGTAAAGG AGTGGCAGGA
    GGAAAGTCCT GCTCAAAAAT CTGCCTTGTC AGAGTTTACC CGGCCAGTCA CCGAGATAAA
    GCGATTAAGG TATACGCAAT CCTAGACGAT CAAAGCAACC GGTCTTTAGC AAAATCCACC
    CTTTTTGACA CCTTCAACAT CATAGGACCC GGTTCTCCAT ACTCCTTAAA GACATGTGCA
    GGTACCGTTG AGACGGCGGG GAGGAAGGCA ACTGGATACA AGGTAGAGTC CATAGACGGG
    CAGACCTGCT TATCACTGCC AACCATACTG GAGTGCAACC AAATTCCCGA CAACCGTTCT
    GAGATACCTA CACCAGAAGT AGCAGCATAC CACCCCCACC TGAGGCGTAT AGCTCACCAG
    ATACCAGAAC TGGATCCAGA AGCACCAATA GCCTTACTCC TAGGAAGAGA CATACTACGT
    GTTCACAAAG CTAGAGGACA GATTAATGGT TCCCAAAACG CTCCATACGC CCAGAGACTT
    GACCTAGGGT GGGTCATCAT AGGAGATGTC TGTCTAGGAG GAGTGCACAA GCCAATCTCT
    GTCAACAGCA TGCTCACCAG CACAGTTGAA AATGGACGTC CCTCCTTATT CCAACCATGT
    CAGAACAGTT TCCTGATAAA AGAATTGCCA CACAGAGATC CTGTCCCTTG TCCCTTCATG
    GATCCTTCTA GTGAAGACCA CGCCTGCGAG GGTGAACAAG ATCACTTAGG GTGTACAGTT
    TTCCATAGGT CTAGAGAAGA CAACAAGGTC GCAATGTCCA TAGAAGACAG GCTGTTCTTA
    GAGATCATGG ATCAAGGAAT GACAAAGGAC AAGTCAAAAA GCTGGGTCGC ACCTTTACCA
    TTTAGACCCC GAAGACAACG CTTACCTGAC AATAAAGAAC TTGTCTACAA AAGATTCATC
    TCCCTTAAGC ACAAACTTCA GAGGACACCA GAGATGAAAG AACATTTCTT TAACTTTATG
    CAAAGAATAT TCCAGAACAA CCATGCAGAA ATAGCACCTG CGCTCCGAGA CTCAGAGGAA
    CGTTGGTACT TACCCACATT CGGCGTATAT CATCCTAAAA AGCCAGGCCA GATACGAGTA
    GTGTTCGACT CAAGTGCCAG GTGCGAAGGT ATATCATTAA ACGATGTCTT ACTGTCTGGT
    CCAGACCTCA ACAACAGACT TCTAGAGGTA CTTCTACGTT TCCGTAAAGA TTCCATAGCA
    TTCATGGCCG ACATACAACA GATGTTCTAC TGCTTCCTCG TCAAGGAGGA GCACAGAAAT
    TACTTGAGGT TCTTCTGGTA CCGCAGCAAT GACCCTAATG AAGACATCGT AGAGTACCGT
    ATGAGGGTGC ACATCTTCGG AAACAGTCCT TCACCTGCAG TCGCTACCTA TGGTCTCAGA
    CTTTCAGCTC AAGAGGGTGA AGCAAAGTAT GGGTCAGATG TCAGATCCTT TGTAGAGAAA
    GATTTCTATG TGGATGACTG CTTGAAATCC ACACCTACGA ATGAGTCGGC AATCAGTCTC
    TTAAAAAGGA CTCAAGAGAT GCTCACTTTA TCTAATCTGA GGTTACACAA AATTGCCTCC
    AACAGCCGTG AGTTAATGGA AGCCTTCTCA ACCCAAGACC ATTCGAGTGA CCTTAAGGAC
    TTGGATCTTG ACTCCGATTC CCTTCCCATG CAGCGGAGTC TTGGTTTGCT CTGGGACTTG
    AAGGCAGACA CCTTTACCTT CCAGATCAGC AAAGAAGAAA AGCCCTTCAC ACGCAGAGGA
    GTTCTGTCCA CGATAAACAG CTTGTACGAT CCTCTGGGAT TTGTAACACC TGTCACCATT
    CAAGGCAAAA TTATGTTAAG GAACCTCACC ACAGAGATGT CTGATTGGGA TGATCCGCTC
    CCCACAGAGA AAAAAGACCT ATGGACAGTT TGGAAAGGGT CTCTTGAAGC TTTGACCAGT
    CTTCATGTGA CACGACCCTA TGCTTCCATA CCATCTACAG AAGTCAAGAG ACAAAAGCTA
    CATATCTTCT GCGATGCTTC AATCAAGGCA ATCGCTGCTG TAGCATACCT AAAGACCATT
    GATGACAAAG AACAATGCCA TGTAGGATTC GTCATGAGTC GGACCAAACT GGCACCTCTC
    CGCGAACACA CAATACCCAG ACTGGAGCTC TGTGCTGCTG TGCTTGCAGT GGAGTTGGCT
    GAACTTATAA CTTCAGGAAT AGGCCTAGAA GTCGAAGAAG TCGAGTTCCA CACGGACAGT
    AAGGTAGTCC TAGGATATAT CAGCAACGAA ACTAGACGCT TTTATGTCTA CGTCTCCAAT
    CGGGTGCTGA GGATCAGGAG ATCCACTAGT CCTCAACAGT GGCACTACGT GCCTACACAA
    CATAATCCTG CAGACCACGC AACCAGATCC ATCACAGCCT GCCACCTGAA AGCCACAACA
    TGGTTCACAG GTCCTGCGTT CCTGTACCGT TCAACTGCTT GCGATATCGG ATACGACACA
    TTTGAATTGA TAGACCCAGA CGCGGATGAA GAGATCCGAC CTGAAGTGTC TGTTCTTAAC
    ACAGTGACTT CTGATCATCA ACTTGAATCT CACCATTTCA GCAGGTTCTC CACCTGGACG
    TCTCTGGTCC GTGCGATAGC CATCTTGATT CATATAGCTA AGTCCTACAC ATCTACATTA
    ACTGTGAATC AGAAACCTTG TAAAGGTTGG CATCACTGCG AGAACACCTT TACTGCTTTC
    AATCTGGAGA AGACAAGTAG TGACGTCCGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.