atp8a2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	atp8a2
Entrez Gene ID	101730757
Full Name	ATPase, aminophospholipid transporter, class I, type 8A, member 2
General protein information	Preferred Names ATPase, aminophospholipid transporter, class I, type 8A, member 2 Names phospholipid-transporting ATPase IB ATPase phospholipid transporting 8A2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031897248.1	XP_031753108.1	phospholipid-transporting ATPase IB isoform X2
XM_012957935.2	XP_012813389.1	phospholipid-transporting ATPase IB
XM_012957935.3	XP_012813389.2	phospholipid-transporting ATPase IB isoform X1
XM_031897249.1	XP_031753109.1	phospholipid-transporting ATPase IB isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ATP8A2	XP_003433345.1
Bos taurus (cattle)	ATP8A2	NP_001157274.3
Gallus gallus (chicken)	ATP8A2	XP_417130.4
Saccharomyces cerevisiae (baker's yeast)	DRS2	NP_009376.2
Neurospora crassa	NCU00352	XP_957823.1
Homo sapiens (human)	ATP8A2	NP_057613.4
Mus musculus (house mouse)	Atp8a2	NP_056618.1
Danio rerio (zebrafish)	atp8a2	XP_005162794.1
Kluyveromyces lactis	KLLA0A04015g	XP_451177.1
Magnaporthe oryzae (rice blast fungus)	MGG_12922	XP_003712724.1
Oryza sativa (rice)	Os10g0412000	NP_001064586.1
Pan troglodytes (chimpanzee)	ATP8A2	XP_522636.3
Schizosaccharomyces pombe (fission yeast)	SPBC887.12	NP_596486.1
Arabidopsis thaliana (thale cress)	ALA3	NP_176191.1
	ATP8A2	XP_001092901.2
Rattus norvegicus (Norway rat)	LOC691889	XP_003751540.1
Eremothecium gossypii	AGOS_ADR350W	NP_984446.2
Xenopus tropicalis (tropical clawed frog)	atp8a2	XP_004912100.1

Gene Ontology

The following atp8a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the atp8a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa52836	XM_031897249.1 Latest version!	Xenopus tropicalis ATPase, aminophospholipid transporter, class I, type 8A, member 2 (atp8a2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa52834	XM_012957935.3 Latest version!	Xenopus tropicalis ATPase, aminophospholipid transporter, class I, type 8A, member 2 (atp8a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa52835	XM_031897248.1 Latest version!	Xenopus tropicalis ATPase, aminophospholipid transporter, class I, type 8A, member 2 (atp8a2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa37504	XM_012957935.2 Latest version!	Xenopus tropicalis ATPase, aminophospholipid transporter, class I, type 8A, member 2 (atp8a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

atp8a2 ( XM_031897249.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753109.1 Xenopus tropicalis ATPase, aminophospholipid transporter, class I, type 8A, member 2 (atp8a2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa52836
Clone ID Related Accession (Same CDS sequence)	XM_031897249.1
Accession Version	XM_031897249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phospholipid-transporting ATPase IB isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTACA TCGAAACATC CAATCTTGAT GGGGAAACGA ACTTAAAAAT ACGACAGGGG 
TTACCAGCAA CATCAAACCT TCTGTCAGAG GAGGACTTGG TGAAAATGAC TGGAAGAATC 
GAATGTGAAG GACCTAATCG ACATCTTTAT GACTTTATTG GAAACCTGCG CCTAGGGACC 
AACAACCCTG TTCCTGTTGG ACCTGAACAA ATTTTGTTGA GAGGTGCCCA GATCAGAAAC 
ACCCAGTGGG TTATTGGAAT AGTTGTGTAC ACTGGACATG ACACCAAATT AATGCAGAAT 
TCAACTAAAG CGCCGCTGAA GAGATCAAAT GTTGAAAAAG TGACCAATGT GCAAATCCTG 
GTTTTATTTG GCATTCTCCT GGTTATGGCT CTTGTCAGCT CAGTTGGAGC CTTATTATGG 
AACTGGAAAT ATGAGGGAAC CAACTGGTAC CTAGGAGATA TTGATGCAGT CTCTACAAAT 
TTTGGTTACA ACCTTTTGAC CTTCATCATT TTGTACAACA ACCTCATTCC TATCAGCCTT 
CTGGTGACTT TGGAAGTAGT TAAGTTTACC CAGGCTCTTT TCATCAACTG GGACAGAGAT 
ATGTATTATT CAGAAACAGA CACCCCTGCC ATGGCAAGAA CATCTAATCT GAATGAGGAG 
CTTGGACAGG TGAAATATCT TTTCTCTGAC AAAACTGGGA CTTTAACATG CAACGTCATG 
CACTTTAAGA AATGCAGCAT TGGTGGAATT ACCTATGGGC ACTTTCCAGA ATTTGAAAGG 
GAACGTTCAT CTGAAGATTT CAGCCAGCTT CCAACCACTA GCGAGTCCTG TGATTTTGAT 
GACCCCAAGC TGTTCCAGAA CATTGAAAAT GATTCTCCAA CAGGAGATAC TATTAAAGAA 
TTTCTCACCT TGTTGGCTGT CTGTCATACT GTTGTCCCCG AGAAAGATGA AGATACAATA 
ATCTACCAGG CCTCCTCTCC AGATGAAGGA GCACTAGTGA AAGGTGCAAG AAAACTTGGC 
TACACTTTTA CTGGGAGAAC TCCGGACTCT GTGATTATTG ATGCAATGGG GAAAGAACAG 
AGCTTTGAAC TCCTCAATGT GCTGGAATTC TCCAGCAACA GGAAGAGGAT GTCTGTTATT 
GTCCGAACTC CATCAGGGAA CCTTCGCCTT TATTGCAAAG GAGCAGATAA TGTTATTTTT 
GAGAGGCTTT CAAGAGAGTC GAAGTATATG GAACAAACCC TAAACCACCT GGAACAATTT 
GCTTCTGAAG GTTTAAGGAC CTTGTGTCTT GCATACTGTG ACCTGCAAGA TTCAGAGTAT 
GAGGCGTGGT TGAACGTTCA TAGTGAAGCC AGCGTGGTTA TTAAGGACAG AGCCCAGAAA 
CTTGAAGAGT GCTATGAAAT TATTGAAAAG GATTTGATGC TGCTTGGTGC AACCGCCATT 
GAAGACCGCC TTCAAGGAGG AGTCCCAGAG ACCATAGCAA CATTAATGAA AGCTGAAATA 
AAAATATGGG TCTTAACAGG AGATAAACAA GAAACAGCAG TCAACATAGG TTATTCGTGC 
AGGCTGGTGT CACAAACAAT GTCGCTCATC GTAGTGAATG AGGATTCTCT GGATGCAACA 
AGGTCAACAC TGACTAATCA CTGTACAAAC CTTGGCGATG GGCTTGGCAA AGAAAACGAC 
CTTGCTCTAA TCATCGACGG GCAAACGCTA AAATACGCGC TTTCATTTGA GGTTCGTCAG 
AGCTTTCTCG ATCTGGCGCT TTCCTGCAAA GCTGTCATTT GCTGCAGAGT GTCTCCGCTG 
CAGAAATCTG AAATAGTAGA CATGGTAAAG AAACATGTGA ATGCCATCAC ACTGGCTATT 
GGAGATGGTG CCAACGATGT AGGAATGATC CAGACCGCCC ACGTAGGAGT CGGGATCAGT 
GGGAACGAGG GCATGCAAGC AACCAACAAC TCCGATTACG CCATAGCGCA GTTTTGCTAT 
TTGGAGAAGC TGCTACTCGT GCACGGAGCT TGGAGCTACA ATCGAGTGAC CAAGTGCATC 
CTATACTGTT TTTACAAGAA TGTGGTTCTC TACATCATCG AGCTCTGGTT TGCAGTCGTT 
AATGGATTTT CTGGACAAGT TTTGTTTGAG CGATGGTGCA TTGGTTTGTA CAATGTGATA 
TTCACCGCCT TACCGCCTTT TACGCTGGGA ATCTGTGAGC GATCGTGCAG CCAAGACAGC 
ATGCTAAGGT TTCCCCAGCT CTACAAGATC ACACAGAATG CCGATGGGTT TAACACACGG 
GTTTTCTGGG GTCACTGCAT CAACGCCTTG ATCCACTCCA TAATTCTCTT CTGGTTTCCA 
CTGAAAATGC TCGAACACGA TGCTGTGTTT ACCAATGGAC AGGTTACAGA CTATTTATTT 
GTTGGAAACA TTGTTTACAC TTACGTTGTA GTGACAGTCT GTTTGAAAGC TGGTTTGGAG 
ACCACGGCAT GGACAAAGTT CAGTCACCTG GCAGTTTGGG GCAGCATGCT GATGTGGCTG 
GTATTTTTTG GAGCCTATTC TGCGATCTGG CCAACTATTC CCATTGCTCC TGATATGCTT 
GGACAGGCCG GGATGGTATT AACGTCGGGG TACTTCTGGC TCGGGTTACT CTTGGTCCCC 
ACTGCATGTC TACTAAGGGA TGTGACCTGG AGAGCGGCAA AGCACACCTA CCACAAGACC 
TTGCTGGAAC AAGTGCAAGA GATTGAGACC AGAGCCAAAG AAATGAGTAA AGCTGCCATG 
CGGGATTCCA ATGGCAAAAG CTTGAACGAA CGTGACCACT TGTTGAGGAG ACTGGGCAGA 
AAAACCCCTC CGAGCCTCTT TCGTGCTAAT TCCGTGCAAC AAAGTGTATC GTACGGCTAT 
GCATTTTCCC AAGAAGAACA CGGTGTTGTT TCCCAGTCTC AAGTTGTCCG ATCCTATGAC 
ACAACAAAAC AGAGAGCCGG GATAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753109.1
CDS	92..3061
Translation	MCYIETSNLDGETNLKIRQGLPATSNLLSEEDLVKMTGRIECEGPNRHLYDFIGNLRLGTNNPVPVGPEQILLRGAQIRNTQWVIGIVVYTGHDTKLMQNSTKAPLKRSNVEKVTNVQILVLFGILLVMALVSSVGALLWNWKYEGTNWYLGDIDAVSTNFGYNLLTFIILYNNLIPISLLVTLEVVKFTQALFINWDRDMYYSETDTPAMARTSNLNEELGQVKYLFSDKTGTLTCNVMHFKKCSIGGITYGHFPEFERERSSEDFSQLPTTSESCDFDDPKLFQNIENDSPTGDTIKEFLTLLAVCHTVVPEKDEDTIIYQASSPDEGALVKGARKLGYTFTGRTPDSVIIDAMGKEQSFELLNVLEFSSNRKRMSVIVRTPSGNLRLYCKGADNVIFERLSRESKYMEQTLNHLEQFASEGLRTLCLAYCDLQDSEYEAWLNVHSEASVVIKDRAQKLEECYEIIEKDLMLLGATAIEDRLQGGVPETIATLMKAEIKIWVLTGDKQETAVNIGYSCRLVSQTMSLIVVNEDSLDATRSTLTNHCTNLGDGLGKENDLALIIDGQTLKYALSFEVRQSFLDLALSCKAVICCRVSPLQKSEIVDMVKKHVNAITLAIGDGANDVGMIQTAHVGVGISGNEGMQATNNSDYAIAQFCYLEKLLLVHGAWSYNRVTKCILYCFYKNVVLYIIELWFAVVNGFSGQVLFERWCIGLYNVIFTALPPFTLGICERSCSQDSMLRFPQLYKITQNADGFNTRVFWGHCINALIHSIILFWFPLKMLEHDAVFTNGQVTDYLFVGNIVYTYVVVTVCLKAGLETTAWTKFSHLAVWGSMLMWLVFFGAYSAIWPTIPIAPDMLGQAGMVLTSGYFWLGLLLVPTACLLRDVTWRAAKHTYHKTLLEQVQEIETRAKEMSKAAMRDSNGKSLNERDHLLRRLGRKTPPSLFRANSVQQSVSYGYAFSQEEHGVVSQSQVVRSYDTTKQRAGIE

Target ORF information:

RefSeq Version	XM_031897249.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ATPase, aminophospholipid transporter, class I, type 8A, member 2 (atp8a2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897249.1

ORF Insert Sequence:

ATGTGCTACA TCGAAACATC CAATCTTGAT GGGGAAACGA ACTTAAAAAT ACGACAGGGG 
TTACCAGCAA CATCAAACCT TCTGTCAGAG GAGGACTTGG TGAAAATGAC TGGAAGAATC 
GAATGTGAAG GACCTAATCG ACATCTTTAT GACTTTATTG GAAACCTGCG CCTAGGGACC 
AACAACCCTG TTCCTGTTGG ACCTGAACAA ATTTTGTTGA GAGGTGCCCA GATCAGAAAC 
ACCCAGTGGG TTATTGGAAT AGTTGTGTAC ACTGGACATG ACACCAAATT AATGCAGAAT 
TCAACTAAAG CGCCGCTGAA GAGATCAAAT GTTGAAAAAG TGACCAATGT GCAAATCCTG 
GTTTTATTTG GCATTCTCCT GGTTATGGCT CTTGTCAGCT CAGTTGGAGC CTTATTATGG 
AACTGGAAAT ATGAGGGAAC CAACTGGTAC CTAGGAGATA TTGATGCAGT CTCTACAAAT 
TTTGGTTACA ACCTTTTGAC CTTCATCATT TTGTACAACA ACCTCATTCC TATCAGCCTT 
CTGGTGACTT TGGAAGTAGT TAAGTTTACC CAGGCTCTTT TCATCAACTG GGACAGAGAT 
ATGTATTATT CAGAAACAGA CACCCCTGCC ATGGCAAGAA CATCTAATCT GAATGAGGAG 
CTTGGACAGG TGAAATATCT TTTCTCTGAC AAAACTGGGA CTTTAACATG CAACGTCATG 
CACTTTAAGA AATGCAGCAT TGGTGGAATT ACCTATGGGC ACTTTCCAGA ATTTGAAAGG 
GAACGTTCAT CTGAAGATTT CAGCCAGCTT CCAACCACTA GCGAGTCCTG TGATTTTGAT 
GACCCCAAGC TGTTCCAGAA CATTGAAAAT GATTCTCCAA CAGGAGATAC TATTAAAGAA 
TTTCTCACCT TGTTGGCTGT CTGTCATACT GTTGTCCCCG AGAAAGATGA AGATACAATA 
ATCTACCAGG CCTCCTCTCC AGATGAAGGA GCACTAGTGA AAGGTGCAAG AAAACTTGGC 
TACACTTTTA CTGGGAGAAC TCCGGACTCT GTGATTATTG ATGCAATGGG GAAAGAACAG 
AGCTTTGAAC TCCTCAATGT GCTGGAATTC TCCAGCAACA GGAAGAGGAT GTCTGTTATT 
GTCCGAACTC CATCAGGGAA CCTTCGCCTT TATTGCAAAG GAGCAGATAA TGTTATTTTT 
GAGAGGCTTT CAAGAGAGTC GAAGTATATG GAACAAACCC TAAACCACCT GGAACAATTT 
GCTTCTGAAG GTTTAAGGAC CTTGTGTCTT GCATACTGTG ACCTGCAAGA TTCAGAGTAT 
GAGGCGTGGT TGAACGTTCA TAGTGAAGCC AGCGTGGTTA TTAAGGACAG AGCCCAGAAA 
CTTGAAGAGT GCTATGAAAT TATTGAAAAG GATTTGATGC TGCTTGGTGC AACCGCCATT 
GAAGACCGCC TTCAAGGAGG AGTCCCAGAG ACCATAGCAA CATTAATGAA AGCTGAAATA 
AAAATATGGG TCTTAACAGG AGATAAACAA GAAACAGCAG TCAACATAGG TTATTCGTGC 
AGGCTGGTGT CACAAACAAT GTCGCTCATC GTAGTGAATG AGGATTCTCT GGATGCAACA 
AGGTCAACAC TGACTAATCA CTGTACAAAC CTTGGCGATG GGCTTGGCAA AGAAAACGAC 
CTTGCTCTAA TCATCGACGG GCAAACGCTA AAATACGCGC TTTCATTTGA GGTTCGTCAG 
AGCTTTCTCG ATCTGGCGCT TTCCTGCAAA GCTGTCATTT GCTGCAGAGT GTCTCCGCTG 
CAGAAATCTG AAATAGTAGA CATGGTAAAG AAACATGTGA ATGCCATCAC ACTGGCTATT 
GGAGATGGTG CCAACGATGT AGGAATGATC CAGACCGCCC ACGTAGGAGT CGGGATCAGT 
GGGAACGAGG GCATGCAAGC AACCAACAAC TCCGATTACG CCATAGCGCA GTTTTGCTAT 
TTGGAGAAGC TGCTACTCGT GCACGGAGCT TGGAGCTACA ATCGAGTGAC CAAGTGCATC 
CTATACTGTT TTTACAAGAA TGTGGTTCTC TACATCATCG AGCTCTGGTT TGCAGTCGTT 
AATGGATTTT CTGGACAAGT TTTGTTTGAG CGATGGTGCA TTGGTTTGTA CAATGTGATA 
TTCACCGCCT TACCGCCTTT TACGCTGGGA ATCTGTGAGC GATCGTGCAG CCAAGACAGC 
ATGCTAAGGT TTCCCCAGCT CTACAAGATC ACACAGAATG CCGATGGGTT TAACACACGG 
GTTTTCTGGG GTCACTGCAT CAACGCCTTG ATCCACTCCA TAATTCTCTT CTGGTTTCCA 
CTGAAAATGC TCGAACACGA TGCTGTGTTT ACCAATGGAC AGGTTACAGA CTATTTATTT 
GTTGGAAACA TTGTTTACAC TTACGTTGTA GTGACAGTCT GTTTGAAAGC TGGTTTGGAG 
ACCACGGCAT GGACAAAGTT CAGTCACCTG GCAGTTTGGG GCAGCATGCT GATGTGGCTG 
GTATTTTTTG GAGCCTATTC TGCGATCTGG CCAACTATTC CCATTGCTCC TGATATGCTT 
GGACAGGCCG GGATGGTATT AACGTCGGGG TACTTCTGGC TCGGGTTACT CTTGGTCCCC 
ACTGCATGTC TACTAAGGGA TGTGACCTGG AGAGCGGCAA AGCACACCTA CCACAAGACC 
TTGCTGGAAC AAGTGCAAGA GATTGAGACC AGAGCCAAAG AAATGAGTAA AGCTGCCATG 
CGGGATTCCA ATGGCAAAAG CTTGAACGAA CGTGACCACT TGTTGAGGAG ACTGGGCAGA 
AAAACCCCTC CGAGCCTCTT TCGTGCTAAT TCCGTGCAAC AAAGTGTATC GTACGGCTAT 
GCATTTTCCC AAGAAGAACA CGGTGTTGTT TCCCAGTCTC AAGTTGTCCG ATCCTATGAC 
ACAACAAAAC AGAGAGCCGG GATAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp8a2 ( XM_012957935.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813389.2 Xenopus tropicalis ATPase, aminophospholipid transporter, class I, type 8A, member 2 (atp8a2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa52834
Clone ID Related Accession (Same CDS sequence)	XM_012957935.3
Accession Version	XM_012957935.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phospholipid-transporting ATPase IB isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957935.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCCT CCTCCCTCCC CCTGCCTTCA CCTCCAGCTC CCCGCCCCTC TCCCCCCTCC 
CCTCTCCGCT TCCCTTTATC TGATCGCTGT GTGCTCCCGC TGGCAGGTCC GCTCCGCTGC 
TCCTCGGGTT ACAAGAAGGC GGATGATGAG ATGTCCGGGG CCACGTCCAG TGCTGACTTG 
GGGGACTCCT CCGGCCGCTG CATCCTCATC AACCAACCCC AGAGCTGCAA GTTCAGGGAC 
AACACAGTCA GCACAGCCAA GTACAGTGTG TTAACGTTTC TACCTCGGTT CCTCTATGAG 
CAGATCAGGA GAGCTGCCAA TGCTTTCTTC CTCTTCATTG CCTTATTACA GCAAATTCCT 
GATGTGTCTC CGACAGGAAG ATATACCACT TTGGTGCCCT TAATCTTTAT TCTCACAGTG 
GCCGGCATCA AGGAAATCAT AGAAGACTAT AAAAGACACA AGGCAGATAA CACTGTTAAT 
AAAAAGAAAA CCATAGTGCT GAAAGGGGGG ACATGGCAGA ACATGGTTTG GAGAGAGGTG 
GCAGTAGGAG ACATTGTGAA GGTTACCAAT GGGCAACATC TTCCAGCTGA TATGATCATT 
TTATCTTCCA GTGAACCACA AGCTATGTGC TACATCGAAA CATCCAATCT TGATGGGGAA 
ACGAACTTAA AAATACGACA GGGGTTACCA GCAACATCAA ACCTTCTGTC AGAGGAGGAC 
TTGGTGAAAA TGACTGGAAG AATCGAATGT GAAGGACCTA ATCGACATCT TTATGACTTT 
ATTGGAAACC TGCGCCTAGG GACCAACAAC CCTGTTCCTG TTGGACCTGA ACAAATTTTG 
TTGAGAGGTG CCCAGATCAG AAACACCCAG TGGGTTATTG GAATAGTTGT GTACACTGGA 
CATGACACCA AATTAATGCA GAATTCAACT AAAGCGCCGC TGAAGAGATC AAATGTTGAA 
AAAGTGACCA ATGTGCAAAT CCTGGTTTTA TTTGGCATTC TCCTGGTTAT GGCTCTTGTC 
AGCTCAGTTG GAGCCTTATT ATGGAACTGG AAATATGAGG GAACCAACTG GTACCTAGGA 
GATATTGATG CAGTCTCTAC AAATTTTGGT TACAACCTTT TGACCTTCAT CATTTTGTAC 
AACAACCTCA TTCCTATCAG CCTTCTGGTG ACTTTGGAAG TAGTTAAGTT TACCCAGGCT 
CTTTTCATCA ACTGGGACAG AGATATGTAT TATTCAGAAA CAGACACCCC TGCCATGGCA 
AGAACATCTA ATCTGAATGA GGAGCTTGGA CAGGTGAAAT ATCTTTTCTC TGACAAAACT 
GGGACTTTAA CATGCAACGT CATGCACTTT AAGAAATGCA GCATTGGTGG AATTACCTAT 
GGGCACTTTC CAGAATTTGA AAGGGAACGT TCATCTGAAG ATTTCAGCCA GCTTCCAACC 
ACTAGCGAGT CCTGTGATTT TGATGACCCC AAGCTGTTCC AGAACATTGA AAATGATTCT 
CCAACAGGAG ATACTATTAA AGAATTTCTC ACCTTGTTGG CTGTCTGTCA TACTGTTGTC 
CCCGAGAAAG ATGAAGATAC AATAATCTAC CAGGCCTCCT CTCCAGATGA AGGAGCACTA 
GTGAAAGGTG CAAGAAAACT TGGCTACACT TTTACTGGGA GAACTCCGGA CTCTGTGATT 
ATTGATGCAA TGGGGAAAGA ACAGAGCTTT GAACTCCTCA ATGTGCTGGA ATTCTCCAGC 
AACAGGAAGA GGATGTCTGT TATTGTCCGA ACTCCATCAG GGAACCTTCG CCTTTATTGC 
AAAGGAGCAG ATAATGTTAT TTTTGAGAGG CTTTCAAGAG AGTCGAAGTA TATGGAACAA 
ACCCTAAACC ACCTGGAACA ATTTGCTTCT GAAGGTTTAA GGACCTTGTG TCTTGCATAC 
TGTGACCTGC AAGATTCAGA GTATGAGGCG TGGTTGAACG TTCATAGTGA AGCCAGCGTG 
GTTATTAAGG ACAGAGCCCA GAAACTTGAA GAGTGCTATG AAATTATTGA AAAGGATTTG 
ATGCTGCTTG GTGCAACCGC CATTGAAGAC CGCCTTCAAG GAGGAGTCCC AGAGACCATA 
GCAACATTAA TGAAAGCTGA AATAAAAATA TGGGTCTTAA CAGGAGATAA ACAAGAAACA 
GCAGTCAACA TAGGTTATTC GTGCAGGCTG GTGTCACAAA CAATGTCGCT CATCGTAGTG 
AATGAGGATT CTCTGGATGC AACAAGGTCA ACACTGACTA ATCACTGTAC AAACCTTGGC 
GATGGGCTTG GCAAAGAAAA CGACCTTGCT CTAATCATCG ACGGGCAAAC GCTAAAATAC 
GCGCTTTCAT TTGAGGTTCG TCAGAGCTTT CTCGATCTGG CGCTTTCCTG CAAAGCTGTC 
ATTTGCTGCA GAGTGTCTCC GCTGCAGAAA TCTGAAATAG TAGACATGGT AAAGAAACAT 
GTGAATGCCA TCACACTGGC TATTGGAGAT GGTGCCAACG ATGTAGGAAT GATCCAGACC 
GCCCACGTAG GAGTCGGGAT CAGTGGGAAC GAGGGCATGC AAGCAACCAA CAACTCCGAT 
TACGCCATAG CGCAGTTTTG CTATTTGGAG AAGCTGCTAC TCGTGCACGG AGCTTGGAGC 
TACAATCGAG TGACCAAGTG CATCCTATAC TGTTTTTACA AGAATGTGGT TCTCTACATC 
ATCGAGCTCT GGTTTGCAGT CGTTAATGGA TTTTCTGGAC AAGTTTTGTT TGAGCGATGG 
TGCATTGGTT TGTACAATGT GATATTCACC GCCTTACCGC CTTTTACGCT GGGAATCTGT 
GAGCGATCGT GCAGCCAAGA CAGCATGCTA AGGTTTCCCC AGCTCTACAA GATCACACAG 
AATGCCGATG GGTTTAACAC ACGGGTTTTC TGGGGTCACT GCATCAACGC CTTGATCCAC 
TCCATAATTC TCTTCTGGTT TCCACTGAAA ATGCTCGAAC ACGATGCTGT GTTTACCAAT 
GGACAGGTTA CAGACTATTT ATTTGTTGGA AACATTGTTT ACACTTACGT TGTAGTGACA 
GTCTGTTTGA AAGCTGGTTT GGAGACCACG GCATGGACAA AGTTCAGTCA CCTGGCAGTT 
TGGGGCAGCA TGCTGATGTG GCTGGTATTT TTTGGAGCCT ATTCTGCGAT CTGGCCAACT 
ATTCCCATTG CTCCTGATAT GCTTGGACAG GCCGGGATGG TATTAACGTC GGGGTACTTC 
TGGCTCGGGT TACTCTTGGT CCCCACTGCA TGTCTACTAA GGGATGTGAC CTGGAGAGCG 
GCAAAGCACA CCTACCACAA GACCTTGCTG GAACAAGTGC AAGAGATTGA GACCAGAGCC 
AAAGAAATGA GTAAAGCTGC CATGCGGGAT TCCAATGGCA AAAGCTTGAA CGAACGTGAC 
CACTTGTTGA GGAGACTGGG CAGAAAAACC CCTCCGAGCC TCTTTCGTGC TAATTCCGTG 
CAACAAAGTG TATCGTACGG CTATGCATTT TCCCAAGAAG AACACGGTGT TGTTTCCCAG 
TCTCAAGTTG TCCGATCCTA TGACACAACA AAACAGAGAG CCGGGATAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813389.2
CDS	33..3626
Translation	MSASSLPLPSPPAPRPSPPSPLRFPLSDRCVLPLAGPLRCSSGYKKADDEMSGATSSADLGDSSGRCILINQPQSCKFRDNTVSTAKYSVLTFLPRFLYEQIRRAANAFFLFIALLQQIPDVSPTGRYTTLVPLIFILTVAGIKEIIEDYKRHKADNTVNKKKTIVLKGGTWQNMVWREVAVGDIVKVTNGQHLPADMIILSSSEPQAMCYIETSNLDGETNLKIRQGLPATSNLLSEEDLVKMTGRIECEGPNRHLYDFIGNLRLGTNNPVPVGPEQILLRGAQIRNTQWVIGIVVYTGHDTKLMQNSTKAPLKRSNVEKVTNVQILVLFGILLVMALVSSVGALLWNWKYEGTNWYLGDIDAVSTNFGYNLLTFIILYNNLIPISLLVTLEVVKFTQALFINWDRDMYYSETDTPAMARTSNLNEELGQVKYLFSDKTGTLTCNVMHFKKCSIGGITYGHFPEFERERSSEDFSQLPTTSESCDFDDPKLFQNIENDSPTGDTIKEFLTLLAVCHTVVPEKDEDTIIYQASSPDEGALVKGARKLGYTFTGRTPDSVIIDAMGKEQSFELLNVLEFSSNRKRMSVIVRTPSGNLRLYCKGADNVIFERLSRESKYMEQTLNHLEQFASEGLRTLCLAYCDLQDSEYEAWLNVHSEASVVIKDRAQKLEECYEIIEKDLMLLGATAIEDRLQGGVPETIATLMKAEIKIWVLTGDKQETAVNIGYSCRLVSQTMSLIVVNEDSLDATRSTLTNHCTNLGDGLGKENDLALIIDGQTLKYALSFEVRQSFLDLALSCKAVICCRVSPLQKSEIVDMVKKHVNAITLAIGDGANDVGMIQTAHVGVGISGNEGMQATNNSDYAIAQFCYLEKLLLVHGAWSYNRVTKCILYCFYKNVVLYIIELWFAVVNGFSGQVLFERWCIGLYNVIFTALPPFTLGICERSCSQDSMLRFPQLYKITQNADGFNTRVFWGHCINALIHSIILFWFPLKMLEHDAVFTNGQVTDYLFVGNIVYTYVVVTVCLKAGLETTAWTKFSHLAVWGSMLMWLVFFGAYSAIWPTIPIAPDMLGQAGMVLTSGYFWLGLLLVPTACLLRDVTWRAAKHTYHKTLLEQVQEIETRAKEMSKAAMRDSNGKSLNERDHLLRRLGRKTPPSLFRANSVQQSVSYGYAFSQEEHGVVSQSQVVRSYDTTKQRAGIE

Target ORF information:

RefSeq Version	XM_012957935.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ATPase, aminophospholipid transporter, class I, type 8A, member 2 (atp8a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957935.3

ORF Insert Sequence:

ATGTCTGCCT CCTCCCTCCC CCTGCCTTCA CCTCCAGCTC CCCGCCCCTC TCCCCCCTCC 
CCTCTCCGCT TCCCTTTATC TGATCGCTGT GTGCTCCCGC TGGCAGGTCC GCTCCGCTGC 
TCCTCGGGTT ACAAGAAGGC GGATGATGAG ATGTCCGGGG CCACGTCCAG TGCTGACTTG 
GGGGACTCCT CCGGCCGCTG CATCCTCATC AACCAACCCC AGAGCTGCAA GTTCAGGGAC 
AACACAGTCA GCACAGCCAA GTACAGTGTG TTAACGTTTC TACCTCGGTT CCTCTATGAG 
CAGATCAGGA GAGCTGCCAA TGCTTTCTTC CTCTTCATTG CCTTATTACA GCAAATTCCT 
GATGTGTCTC CGACAGGAAG ATATACCACT TTGGTGCCCT TAATCTTTAT TCTCACAGTG 
GCCGGCATCA AGGAAATCAT AGAAGACTAT AAAAGACACA AGGCAGATAA CACTGTTAAT 
AAAAAGAAAA CCATAGTGCT GAAAGGGGGG ACATGGCAGA ACATGGTTTG GAGAGAGGTG 
GCAGTAGGAG ACATTGTGAA GGTTACCAAT GGGCAACATC TTCCAGCTGA TATGATCATT 
TTATCTTCCA GTGAACCACA AGCTATGTGC TACATCGAAA CATCCAATCT TGATGGGGAA 
ACGAACTTAA AAATACGACA GGGGTTACCA GCAACATCAA ACCTTCTGTC AGAGGAGGAC 
TTGGTGAAAA TGACTGGAAG AATCGAATGT GAAGGACCTA ATCGACATCT TTATGACTTT 
ATTGGAAACC TGCGCCTAGG GACCAACAAC CCTGTTCCTG TTGGACCTGA ACAAATTTTG 
TTGAGAGGTG CCCAGATCAG AAACACCCAG TGGGTTATTG GAATAGTTGT GTACACTGGA 
CATGACACCA AATTAATGCA GAATTCAACT AAAGCGCCGC TGAAGAGATC AAATGTTGAA 
AAAGTGACCA ATGTGCAAAT CCTGGTTTTA TTTGGCATTC TCCTGGTTAT GGCTCTTGTC 
AGCTCAGTTG GAGCCTTATT ATGGAACTGG AAATATGAGG GAACCAACTG GTACCTAGGA 
GATATTGATG CAGTCTCTAC AAATTTTGGT TACAACCTTT TGACCTTCAT CATTTTGTAC 
AACAACCTCA TTCCTATCAG CCTTCTGGTG ACTTTGGAAG TAGTTAAGTT TACCCAGGCT 
CTTTTCATCA ACTGGGACAG AGATATGTAT TATTCAGAAA CAGACACCCC TGCCATGGCA 
AGAACATCTA ATCTGAATGA GGAGCTTGGA CAGGTGAAAT ATCTTTTCTC TGACAAAACT 
GGGACTTTAA CATGCAACGT CATGCACTTT AAGAAATGCA GCATTGGTGG AATTACCTAT 
GGGCACTTTC CAGAATTTGA AAGGGAACGT TCATCTGAAG ATTTCAGCCA GCTTCCAACC 
ACTAGCGAGT CCTGTGATTT TGATGACCCC AAGCTGTTCC AGAACATTGA AAATGATTCT 
CCAACAGGAG ATACTATTAA AGAATTTCTC ACCTTGTTGG CTGTCTGTCA TACTGTTGTC 
CCCGAGAAAG ATGAAGATAC AATAATCTAC CAGGCCTCCT CTCCAGATGA AGGAGCACTA 
GTGAAAGGTG CAAGAAAACT TGGCTACACT TTTACTGGGA GAACTCCGGA CTCTGTGATT 
ATTGATGCAA TGGGGAAAGA ACAGAGCTTT GAACTCCTCA ATGTGCTGGA ATTCTCCAGC 
AACAGGAAGA GGATGTCTGT TATTGTCCGA ACTCCATCAG GGAACCTTCG CCTTTATTGC 
AAAGGAGCAG ATAATGTTAT TTTTGAGAGG CTTTCAAGAG AGTCGAAGTA TATGGAACAA 
ACCCTAAACC ACCTGGAACA ATTTGCTTCT GAAGGTTTAA GGACCTTGTG TCTTGCATAC 
TGTGACCTGC AAGATTCAGA GTATGAGGCG TGGTTGAACG TTCATAGTGA AGCCAGCGTG 
GTTATTAAGG ACAGAGCCCA GAAACTTGAA GAGTGCTATG AAATTATTGA AAAGGATTTG 
ATGCTGCTTG GTGCAACCGC CATTGAAGAC CGCCTTCAAG GAGGAGTCCC AGAGACCATA 
GCAACATTAA TGAAAGCTGA AATAAAAATA TGGGTCTTAA CAGGAGATAA ACAAGAAACA 
GCAGTCAACA TAGGTTATTC GTGCAGGCTG GTGTCACAAA CAATGTCGCT CATCGTAGTG 
AATGAGGATT CTCTGGATGC AACAAGGTCA ACACTGACTA ATCACTGTAC AAACCTTGGC 
GATGGGCTTG GCAAAGAAAA CGACCTTGCT CTAATCATCG ACGGGCAAAC GCTAAAATAC 
GCGCTTTCAT TTGAGGTTCG TCAGAGCTTT CTCGATCTGG CGCTTTCCTG CAAAGCTGTC 
ATTTGCTGCA GAGTGTCTCC GCTGCAGAAA TCTGAAATAG TAGACATGGT AAAGAAACAT 
GTGAATGCCA TCACACTGGC TATTGGAGAT GGTGCCAACG ATGTAGGAAT GATCCAGACC 
GCCCACGTAG GAGTCGGGAT CAGTGGGAAC GAGGGCATGC AAGCAACCAA CAACTCCGAT 
TACGCCATAG CGCAGTTTTG CTATTTGGAG AAGCTGCTAC TCGTGCACGG AGCTTGGAGC 
TACAATCGAG TGACCAAGTG CATCCTATAC TGTTTTTACA AGAATGTGGT TCTCTACATC 
ATCGAGCTCT GGTTTGCAGT CGTTAATGGA TTTTCTGGAC AAGTTTTGTT TGAGCGATGG 
TGCATTGGTT TGTACAATGT GATATTCACC GCCTTACCGC CTTTTACGCT GGGAATCTGT 
GAGCGATCGT GCAGCCAAGA CAGCATGCTA AGGTTTCCCC AGCTCTACAA GATCACACAG 
AATGCCGATG GGTTTAACAC ACGGGTTTTC TGGGGTCACT GCATCAACGC CTTGATCCAC 
TCCATAATTC TCTTCTGGTT TCCACTGAAA ATGCTCGAAC ACGATGCTGT GTTTACCAAT 
GGACAGGTTA CAGACTATTT ATTTGTTGGA AACATTGTTT ACACTTACGT TGTAGTGACA 
GTCTGTTTGA AAGCTGGTTT GGAGACCACG GCATGGACAA AGTTCAGTCA CCTGGCAGTT 
TGGGGCAGCA TGCTGATGTG GCTGGTATTT TTTGGAGCCT ATTCTGCGAT CTGGCCAACT 
ATTCCCATTG CTCCTGATAT GCTTGGACAG GCCGGGATGG TATTAACGTC GGGGTACTTC 
TGGCTCGGGT TACTCTTGGT CCCCACTGCA TGTCTACTAA GGGATGTGAC CTGGAGAGCG 
GCAAAGCACA CCTACCACAA GACCTTGCTG GAACAAGTGC AAGAGATTGA GACCAGAGCC 
AAAGAAATGA GTAAAGCTGC CATGCGGGAT TCCAATGGCA AAAGCTTGAA CGAACGTGAC 
CACTTGTTGA GGAGACTGGG CAGAAAAACC CCTCCGAGCC TCTTTCGTGC TAATTCCGTG 
CAACAAAGTG TATCGTACGG CTATGCATTT TCCCAAGAAG AACACGGTGT TGTTTCCCAG 
TCTCAAGTTG TCCGATCCTA TGACACAACA AAACAGAGAG CCGGGATAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp8a2 ( XM_031897248.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753108.1 Xenopus tropicalis ATPase, aminophospholipid transporter, class I, type 8A, member 2 (atp8a2), transcript variant X2, mRNA.

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CloneID	OXa52835
Clone ID Related Accession (Same CDS sequence)	XM_031897248.1
Accession Version	XM_031897248.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phospholipid-transporting ATPase IB isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTTGC AGAGGAAATC AAAGAAGATC CATTCCTCAG GGGGTCCGCT CCGCTGCTCC 
TCGGGTTACA AGAAGGCGGA TGATGAGATG TCCGGGGCCA CGTCCAGTGC TGACTTGGGG 
GACTCCTCCG GCCGCTGCAT CCTCATCAAC CAACCCCAGA GCTGCAAGTT CAGGGACAAC 
ACAGTCAGCA CAGCCAAGTA CAGTGTGTTA ACGTTTCTAC CTCGGTTCCT CTATGAGCAG 
ATCAGGAGAG CTGCCAATGC TTTCTTCCTC TTCATTGCCT TATTACAGCA AATTCCTGAT 
GTGTCTCCGA CAGGAAGATA TACCACTTTG GTGCCCTTAA TCTTTATTCT CACAGTGGCC 
GGCATCAAGG AAATCATAGA AGACTATAAA AGACACAAGG CAGATAACAC TGTTAATAAA 
AAGAAAACCA TAGTGCTGAA AGGGGGGACA TGGCAGAACA TGGTTTGGAG AGAGGTGGCA 
GTAGGAGACA TTGTGAAGGT TACCAATGGG CAACATCTTC CAGCTGATAT GATCATTTTA 
TCTTCCAGTG AACCACAAGC TATGTGCTAC ATCGAAACAT CCAATCTTGA TGGGGAAACG 
AACTTAAAAA TACGACAGGG GTTACCAGCA ACATCAAACC TTCTGTCAGA GGAGGACTTG 
GTGAAAATGA CTGGAAGAAT CGAATGTGAA GGACCTAATC GACATCTTTA TGACTTTATT 
GGAAACCTGC GCCTAGGGAC CAACAACCCT GTTCCTGTTG GACCTGAACA AATTTTGTTG 
AGAGGTGCCC AGATCAGAAA CACCCAGTGG GTTATTGGAA TAGTTGTGTA CACTGGACAT 
GACACCAAAT TAATGCAGAA TTCAACTAAA GCGCCGCTGA AGAGATCAAA TGTTGAAAAA 
GTGACCAATG TGCAAATCCT GGTTTTATTT GGCATTCTCC TGGTTATGGC TCTTGTCAGC 
TCAGTTGGAG CCTTATTATG GAACTGGAAA TATGAGGGAA CCAACTGGTA CCTAGGAGAT 
ATTGATGCAG TCTCTACAAA TTTTGGTTAC AACCTTTTGA CCTTCATCAT TTTGTACAAC 
AACCTCATTC CTATCAGCCT TCTGGTGACT TTGGAAGTAG TTAAGTTTAC CCAGGCTCTT 
TTCATCAACT GGGACAGAGA TATGTATTAT TCAGAAACAG ACACCCCTGC CATGGCAAGA 
ACATCTAATC TGAATGAGGA GCTTGGACAG GTGAAATATC TTTTCTCTGA CAAAACTGGG 
ACTTTAACAT GCAACGTCAT GCACTTTAAG AAATGCAGCA TTGGTGGAAT TACCTATGGG 
CACTTTCCAG AATTTGAAAG GGAACGTTCA TCTGAAGATT TCAGCCAGCT TCCAACCACT 
AGCGAGTCCT GTGATTTTGA TGACCCCAAG CTGTTCCAGA ACATTGAAAA TGATTCTCCA 
ACAGGAGATA CTATTAAAGA ATTTCTCACC TTGTTGGCTG TCTGTCATAC TGTTGTCCCC 
GAGAAAGATG AAGATACAAT AATCTACCAG GCCTCCTCTC CAGATGAAGG AGCACTAGTG 
AAAGGTGCAA GAAAACTTGG CTACACTTTT ACTGGGAGAA CTCCGGACTC TGTGATTATT 
GATGCAATGG GGAAAGAACA GAGCTTTGAA CTCCTCAATG TGCTGGAATT CTCCAGCAAC 
AGGAAGAGGA TGTCTGTTAT TGTCCGAACT CCATCAGGGA ACCTTCGCCT TTATTGCAAA 
GGAGCAGATA ATGTTATTTT TGAGAGGCTT TCAAGAGAGT CGAAGTATAT GGAACAAACC 
CTAAACCACC TGGAACAATT TGCTTCTGAA GGTTTAAGGA CCTTGTGTCT TGCATACTGT 
GACCTGCAAG ATTCAGAGTA TGAGGCGTGG TTGAACGTTC ATAGTGAAGC CAGCGTGGTT 
ATTAAGGACA GAGCCCAGAA ACTTGAAGAG TGCTATGAAA TTATTGAAAA GGATTTGATG 
CTGCTTGGTG CAACCGCCAT TGAAGACCGC CTTCAAGGAG GAGTCCCAGA GACCATAGCA 
ACATTAATGA AAGCTGAAAT AAAAATATGG GTCTTAACAG GAGATAAACA AGAAACAGCA 
GTCAACATAG GTTATTCGTG CAGGCTGGTG TCACAAACAA TGTCGCTCAT CGTAGTGAAT 
GAGGATTCTC TGGATGCAAC AAGGTCAACA CTGACTAATC ACTGTACAAA CCTTGGCGAT 
GGGCTTGGCA AAGAAAACGA CCTTGCTCTA ATCATCGACG GGCAAACGCT AAAATACGCG 
CTTTCATTTG AGGTTCGTCA GAGCTTTCTC GATCTGGCGC TTTCCTGCAA AGCTGTCATT 
TGCTGCAGAG TGTCTCCGCT GCAGAAATCT GAAATAGTAG ACATGGTAAA GAAACATGTG 
AATGCCATCA CACTGGCTAT TGGAGATGGT GCCAACGATG TAGGAATGAT CCAGACCGCC 
CACGTAGGAG TCGGGATCAG TGGGAACGAG GGCATGCAAG CAACCAACAA CTCCGATTAC 
GCCATAGCGC AGTTTTGCTA TTTGGAGAAG CTGCTACTCG TGCACGGAGC TTGGAGCTAC 
AATCGAGTGA CCAAGTGCAT CCTATACTGT TTTTACAAGA ATGTGGTTCT CTACATCATC 
GAGCTCTGGT TTGCAGTCGT TAATGGATTT TCTGGACAAG TTTTGTTTGA GCGATGGTGC 
ATTGGTTTGT ACAATGTGAT ATTCACCGCC TTACCGCCTT TTACGCTGGG AATCTGTGAG 
CGATCGTGCA GCCAAGACAG CATGCTAAGG TTTCCCCAGC TCTACAAGAT CACACAGAAT 
GCCGATGGGT TTAACACACG GGTTTTCTGG GGTCACTGCA TCAACGCCTT GATCCACTCC 
ATAATTCTCT TCTGGTTTCC ACTGAAAATG CTCGAACACG ATGCTGTGTT TACCAATGGA 
CAGGTTACAG ACTATTTATT TGTTGGAAAC ATTGTTTACA CTTACGTTGT AGTGACAGTC 
TGTTTGAAAG CTGGTTTGGA GACCACGGCA TGGACAAAGT TCAGTCACCT GGCAGTTTGG 
GGCAGCATGC TGATGTGGCT GGTATTTTTT GGAGCCTATT CTGCGATCTG GCCAACTATT 
CCCATTGCTC CTGATATGCT TGGACAGGCC GGGATGGTAT TAACGTCGGG GTACTTCTGG 
CTCGGGTTAC TCTTGGTCCC CACTGCATGT CTACTAAGGG ATGTGACCTG GAGAGCGGCA 
AAGCACACCT ACCACAAGAC CTTGCTGGAA CAAGTGCAAG AGATTGAGAC CAGAGCCAAA 
GAAATGAGTA AAGCTGCCAT GCGGGATTCC AATGGCAAAA GCTTGAACGA ACGTGACCAC 
TTGTTGAGGA GACTGGGCAG AAAAACCCCT CCGAGCCTCT TTCGTGCTAA TTCCGTGCAA 
CAAAGTGTAT CGTACGGCTA TGCATTTTCC CAAGAAGAAC ACGGTGTTGT TTCCCAGTCT 
CAAGTTGTCC GATCCTATGA CACAACAAAA CAGAGAGCCG GGATAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753108.1
CDS	60..3590
Translation	MSLQRKSKKIHSSGGPLRCSSGYKKADDEMSGATSSADLGDSSGRCILINQPQSCKFRDNTVSTAKYSVLTFLPRFLYEQIRRAANAFFLFIALLQQIPDVSPTGRYTTLVPLIFILTVAGIKEIIEDYKRHKADNTVNKKKTIVLKGGTWQNMVWREVAVGDIVKVTNGQHLPADMIILSSSEPQAMCYIETSNLDGETNLKIRQGLPATSNLLSEEDLVKMTGRIECEGPNRHLYDFIGNLRLGTNNPVPVGPEQILLRGAQIRNTQWVIGIVVYTGHDTKLMQNSTKAPLKRSNVEKVTNVQILVLFGILLVMALVSSVGALLWNWKYEGTNWYLGDIDAVSTNFGYNLLTFIILYNNLIPISLLVTLEVVKFTQALFINWDRDMYYSETDTPAMARTSNLNEELGQVKYLFSDKTGTLTCNVMHFKKCSIGGITYGHFPEFERERSSEDFSQLPTTSESCDFDDPKLFQNIENDSPTGDTIKEFLTLLAVCHTVVPEKDEDTIIYQASSPDEGALVKGARKLGYTFTGRTPDSVIIDAMGKEQSFELLNVLEFSSNRKRMSVIVRTPSGNLRLYCKGADNVIFERLSRESKYMEQTLNHLEQFASEGLRTLCLAYCDLQDSEYEAWLNVHSEASVVIKDRAQKLEECYEIIEKDLMLLGATAIEDRLQGGVPETIATLMKAEIKIWVLTGDKQETAVNIGYSCRLVSQTMSLIVVNEDSLDATRSTLTNHCTNLGDGLGKENDLALIIDGQTLKYALSFEVRQSFLDLALSCKAVICCRVSPLQKSEIVDMVKKHVNAITLAIGDGANDVGMIQTAHVGVGISGNEGMQATNNSDYAIAQFCYLEKLLLVHGAWSYNRVTKCILYCFYKNVVLYIIELWFAVVNGFSGQVLFERWCIGLYNVIFTALPPFTLGICERSCSQDSMLRFPQLYKITQNADGFNTRVFWGHCINALIHSIILFWFPLKMLEHDAVFTNGQVTDYLFVGNIVYTYVVVTVCLKAGLETTAWTKFSHLAVWGSMLMWLVFFGAYSAIWPTIPIAPDMLGQAGMVLTSGYFWLGLLLVPTACLLRDVTWRAAKHTYHKTLLEQVQEIETRAKEMSKAAMRDSNGKSLNERDHLLRRLGRKTPPSLFRANSVQQSVSYGYAFSQEEHGVVSQSQVVRSYDTTKQRAGIE

Target ORF information:

RefSeq Version	XM_031897248.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ATPase, aminophospholipid transporter, class I, type 8A, member 2 (atp8a2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897248.1

ORF Insert Sequence:

ATGTCCTTGC AGAGGAAATC AAAGAAGATC CATTCCTCAG GGGGTCCGCT CCGCTGCTCC 
TCGGGTTACA AGAAGGCGGA TGATGAGATG TCCGGGGCCA CGTCCAGTGC TGACTTGGGG 
GACTCCTCCG GCCGCTGCAT CCTCATCAAC CAACCCCAGA GCTGCAAGTT CAGGGACAAC 
ACAGTCAGCA CAGCCAAGTA CAGTGTGTTA ACGTTTCTAC CTCGGTTCCT CTATGAGCAG 
ATCAGGAGAG CTGCCAATGC TTTCTTCCTC TTCATTGCCT TATTACAGCA AATTCCTGAT 
GTGTCTCCGA CAGGAAGATA TACCACTTTG GTGCCCTTAA TCTTTATTCT CACAGTGGCC 
GGCATCAAGG AAATCATAGA AGACTATAAA AGACACAAGG CAGATAACAC TGTTAATAAA 
AAGAAAACCA TAGTGCTGAA AGGGGGGACA TGGCAGAACA TGGTTTGGAG AGAGGTGGCA 
GTAGGAGACA TTGTGAAGGT TACCAATGGG CAACATCTTC CAGCTGATAT GATCATTTTA 
TCTTCCAGTG AACCACAAGC TATGTGCTAC ATCGAAACAT CCAATCTTGA TGGGGAAACG 
AACTTAAAAA TACGACAGGG GTTACCAGCA ACATCAAACC TTCTGTCAGA GGAGGACTTG 
GTGAAAATGA CTGGAAGAAT CGAATGTGAA GGACCTAATC GACATCTTTA TGACTTTATT 
GGAAACCTGC GCCTAGGGAC CAACAACCCT GTTCCTGTTG GACCTGAACA AATTTTGTTG 
AGAGGTGCCC AGATCAGAAA CACCCAGTGG GTTATTGGAA TAGTTGTGTA CACTGGACAT 
GACACCAAAT TAATGCAGAA TTCAACTAAA GCGCCGCTGA AGAGATCAAA TGTTGAAAAA 
GTGACCAATG TGCAAATCCT GGTTTTATTT GGCATTCTCC TGGTTATGGC TCTTGTCAGC 
TCAGTTGGAG CCTTATTATG GAACTGGAAA TATGAGGGAA CCAACTGGTA CCTAGGAGAT 
ATTGATGCAG TCTCTACAAA TTTTGGTTAC AACCTTTTGA CCTTCATCAT TTTGTACAAC 
AACCTCATTC CTATCAGCCT TCTGGTGACT TTGGAAGTAG TTAAGTTTAC CCAGGCTCTT 
TTCATCAACT GGGACAGAGA TATGTATTAT TCAGAAACAG ACACCCCTGC CATGGCAAGA 
ACATCTAATC TGAATGAGGA GCTTGGACAG GTGAAATATC TTTTCTCTGA CAAAACTGGG 
ACTTTAACAT GCAACGTCAT GCACTTTAAG AAATGCAGCA TTGGTGGAAT TACCTATGGG 
CACTTTCCAG AATTTGAAAG GGAACGTTCA TCTGAAGATT TCAGCCAGCT TCCAACCACT 
AGCGAGTCCT GTGATTTTGA TGACCCCAAG CTGTTCCAGA ACATTGAAAA TGATTCTCCA 
ACAGGAGATA CTATTAAAGA ATTTCTCACC TTGTTGGCTG TCTGTCATAC TGTTGTCCCC 
GAGAAAGATG AAGATACAAT AATCTACCAG GCCTCCTCTC CAGATGAAGG AGCACTAGTG 
AAAGGTGCAA GAAAACTTGG CTACACTTTT ACTGGGAGAA CTCCGGACTC TGTGATTATT 
GATGCAATGG GGAAAGAACA GAGCTTTGAA CTCCTCAATG TGCTGGAATT CTCCAGCAAC 
AGGAAGAGGA TGTCTGTTAT TGTCCGAACT CCATCAGGGA ACCTTCGCCT TTATTGCAAA 
GGAGCAGATA ATGTTATTTT TGAGAGGCTT TCAAGAGAGT CGAAGTATAT GGAACAAACC 
CTAAACCACC TGGAACAATT TGCTTCTGAA GGTTTAAGGA CCTTGTGTCT TGCATACTGT 
GACCTGCAAG ATTCAGAGTA TGAGGCGTGG TTGAACGTTC ATAGTGAAGC CAGCGTGGTT 
ATTAAGGACA GAGCCCAGAA ACTTGAAGAG TGCTATGAAA TTATTGAAAA GGATTTGATG 
CTGCTTGGTG CAACCGCCAT TGAAGACCGC CTTCAAGGAG GAGTCCCAGA GACCATAGCA 
ACATTAATGA AAGCTGAAAT AAAAATATGG GTCTTAACAG GAGATAAACA AGAAACAGCA 
GTCAACATAG GTTATTCGTG CAGGCTGGTG TCACAAACAA TGTCGCTCAT CGTAGTGAAT 
GAGGATTCTC TGGATGCAAC AAGGTCAACA CTGACTAATC ACTGTACAAA CCTTGGCGAT 
GGGCTTGGCA AAGAAAACGA CCTTGCTCTA ATCATCGACG GGCAAACGCT AAAATACGCG 
CTTTCATTTG AGGTTCGTCA GAGCTTTCTC GATCTGGCGC TTTCCTGCAA AGCTGTCATT 
TGCTGCAGAG TGTCTCCGCT GCAGAAATCT GAAATAGTAG ACATGGTAAA GAAACATGTG 
AATGCCATCA CACTGGCTAT TGGAGATGGT GCCAACGATG TAGGAATGAT CCAGACCGCC 
CACGTAGGAG TCGGGATCAG TGGGAACGAG GGCATGCAAG CAACCAACAA CTCCGATTAC 
GCCATAGCGC AGTTTTGCTA TTTGGAGAAG CTGCTACTCG TGCACGGAGC TTGGAGCTAC 
AATCGAGTGA CCAAGTGCAT CCTATACTGT TTTTACAAGA ATGTGGTTCT CTACATCATC 
GAGCTCTGGT TTGCAGTCGT TAATGGATTT TCTGGACAAG TTTTGTTTGA GCGATGGTGC 
ATTGGTTTGT ACAATGTGAT ATTCACCGCC TTACCGCCTT TTACGCTGGG AATCTGTGAG 
CGATCGTGCA GCCAAGACAG CATGCTAAGG TTTCCCCAGC TCTACAAGAT CACACAGAAT 
GCCGATGGGT TTAACACACG GGTTTTCTGG GGTCACTGCA TCAACGCCTT GATCCACTCC 
ATAATTCTCT TCTGGTTTCC ACTGAAAATG CTCGAACACG ATGCTGTGTT TACCAATGGA 
CAGGTTACAG ACTATTTATT TGTTGGAAAC ATTGTTTACA CTTACGTTGT AGTGACAGTC 
TGTTTGAAAG CTGGTTTGGA GACCACGGCA TGGACAAAGT TCAGTCACCT GGCAGTTTGG 
GGCAGCATGC TGATGTGGCT GGTATTTTTT GGAGCCTATT CTGCGATCTG GCCAACTATT 
CCCATTGCTC CTGATATGCT TGGACAGGCC GGGATGGTAT TAACGTCGGG GTACTTCTGG 
CTCGGGTTAC TCTTGGTCCC CACTGCATGT CTACTAAGGG ATGTGACCTG GAGAGCGGCA 
AAGCACACCT ACCACAAGAC CTTGCTGGAA CAAGTGCAAG AGATTGAGAC CAGAGCCAAA 
GAAATGAGTA AAGCTGCCAT GCGGGATTCC AATGGCAAAA GCTTGAACGA ACGTGACCAC 
TTGTTGAGGA GACTGGGCAG AAAAACCCCT CCGAGCCTCT TTCGTGCTAA TTCCGTGCAA 
CAAAGTGTAT CGTACGGCTA TGCATTTTCC CAAGAAGAAC ACGGTGTTGT TTCCCAGTCT 
CAAGTTGTCC GATCCTATGA CACAACAAAA CAGAGAGCCG GGATAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp8a2 ( XM_012957935.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813389.1 Xenopus tropicalis ATPase, aminophospholipid transporter, class I, type 8A, member 2 (atp8a2), mRNA.

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CloneID	OXa37504
Clone ID Related Accession (Same CDS sequence)	XM_012957935.2
Accession Version	XM_012957935.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phospholipid-transporting ATPase IB
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957935.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCCT CCTCCCTCCC CCTGCCTTCA CCTCCAGCTC CCCGCCCCTC TCCCCCCTCC 
CCTCTCCGCT TCCCTTTATC TGATCGCTGT GTGCTCCCGC TGGCAGGTCC GCTCCGCTGC 
TCCTCGGGTT ACAAGAAGGC GGATGATGAG ATGTCCGGGG CCACGTCCAG TGCTGACTTG 
GGGGACTCCT CCGGCCGCTG CATCCTCATC AACCAACCCC AGAGCTGCAA GTTCAGGGAC 
AACACAGTCA GCACAGCCAA GTACAGTGTG TTAACGTTTC TACCTCGGTT CCTCTATGAG 
CAGATCAGGA GAGCTGCCAA TGCTTTCTTC CTCTTCATTG CCTTATTACA GCAAATTCCT 
GATGTGTCTC CGACAGGAAG ATATACCACT TTGGTGCCCT TAATCTTTAT TCTCACAGTG 
GCCGGCATCA AGGAAATCAT AGAAGACTAT AAAAGACACA AGGCAGATAA CACTGTTAAT 
AAAAAGAAAA CCATAGTGCT GAAAGGGGGG ACATGGCAGA ACATGGTTTG GAGAGAGGTG 
GCAGTAGGAG ACATTGTGAA GGTTACCAAT GGGCAACATC TTCCAGCTGA TATGATCATT 
TTATCTTCCA GTGAACCACA AGCTATGTGC TACATCGAAA CATCCAATCT TGATGGGGAA 
ACGAACTTAA AAATACGACA GGGGTTACCA GCAACATCAA ACCTTCTGTC AGAGGAGGAC 
TTGGTGAAAA TGACTGGAAG AATCGAATGT GAAGGACCTA ATCGACATCT TTATGACTTT 
ATTGGAAACC TGCGCCTAGG GACCAACAAC CCTGTTCCTG TTGGACCTGA ACAAATTTTG 
TTGAGAGGTG CCCAGATCAG AAACACCCAG TGGGTTATTG GAATAGTTGT GTACACTGGA 
CATGACACCA AATTAATGCA GAATTCAACT AAAGCGCCGC TGAAGAGATC AAATGTTGAA 
AAAGTGACCA ATGTGCAAAT CCTGGTTTTA TTTGGCATTC TCCTGGTTAT GGCTCTTGTC 
AGCTCAGTTG GAGCCTTATT ATGGAACTGG AAATATGAGG GAACCAACTG GTACCTAGGA 
GATATTGATG CAGTCTCTAC AAATTTTGGT TACAACCTTT TGACCTTCAT CATTTTGTAC 
AACAATCTCA TTCCTATCAG CCTTCTGGTG ACTTTGGAAG TAGTTAAGTT TACCCAGGCT 
CTTTTCATCA ACTGGGACAG AGATATGTAT TATTCAGAAA CAGACACCCC TGCCATGGCA 
AGAACATCTA ATCTGAATGA GGAGCTTGGA CAGGTGAAAT ATCTTTTCTC TGACAAAACT 
GGGACTTTAA CATGCAACGT CATGCACTTT AAGAAATGCA GCATTGGTGG AATTACCTAT 
GGGCACTTTC CAGAATTTGA AAGGGAACGT TCATCTGAAG ATTTCAGCCA GCTTCCAACC 
ACTAGCGAGT CCTGTGATTT TGATGACCCC AAGCTGTTCC AGAACATTGA AAGTGATTCT 
CCAACAGGAG ATACTATTAA AGAATTTCTC ACCTTGTTGG CTGTCTGTCA TACTGTTGTC 
CCCGAGAAAG ATGAAGATAC AATAATCTAC CAGGCCTCCT CTCCAGATGA AGGAGCACTA 
GTGAAAGGTG CAAGAAAACT TGGCTACACT TTTACTGGGA GAACTCCGGA CTCTGTGATT 
ATTGATGCAA TGGGGAAAGA ACAGAGCTTT GAACTCCTCA ATGTGCTGGA ATTCTCCAGC 
AACAGGAAGA GGATGTCTGT TATTGTCCGA ACTCCATCAG GGAACCTTCG CCTTTATTGC 
AAAGGAGCAG ATAATGTTAT TTTTGAGAGG CTTTCAAGAG AGTCGAAGTA TATGGAACAA 
ACCCTAAACC ACCTGGAACA ATTTGCTTCT GAAGGCTTAA GGACCTTGTG TCTTGCATAC 
TGTGACCTGC AAGATTCGGA GTATCAGGAA TGGTTGAACG TTCATAGTGA AGCCAGCGTG 
GTTATTAAGG ACAGAGCCCA AAAACTTGAA GAGTGCTATG AAATTATTGA AAAGGATTTG 
ATGCTGCTTG GTGCAACCGC CATTGAAGAC CGCCTTCAAG GAGGAGTCCC AGAGACCATA 
GCAACATTAA TGAAAGCTGA AATAAAAATA TGGGTCTTAA CAGGAGATAA ACAAGAAACA 
GCAGTCAACA TAGGTTATTC GTGCAGGCTG GTGTCACAAA CAATGTCGCT CATCGTAGTG 
AATGAGGATT CTCTGGATGC AACAAGGTCA ACACTGACTA ATCACTGTAC AAACCTTGGC 
GATGGGCTTG GCAAAGAAAA CGACCTTGCT CTAATCATCG ACGGGCAAAC GCTAAAATAC 
GCGCTTTCAT TTGAGGTTCG TCAGAGCTTT CTCGATCTGG CGCTTTCCTG CAAAGCTGTC 
ATTTGCTGCA GAGTGTCTCC GCTGCAGAAA TCTGAAATAG TAGACATGGT AAAGAAACAT 
GTGAATGCCA TCACACTGGC TATTGGAGAT GGTGCCAACG ATGTAGGAAT GATCCAGACC 
GCCCACGTAG GAGTCGGGAT CAGTGGGAAC GAGGGCATGC AAGCAACCAA CAACTCCGAT 
TACGCCATAG CGCAGTTTTG CTATTTGGAG AAGCTGCTAC TCGTGCACGG AGCTTGGAGC 
TACAATCGAG TGACCAAGTG CATCCTATAC TGTTTTTACA AGAATGTGGT TCTCTACATC 
ATCGAGCTCT GGTTTGCAGT CGTTAATGGA TTTTCTGGAC AAGTTTTGTT TGAGCGATGG 
TGCATTGGTT TGTACAATGT GATATTCACC GCCTTACCGC CTTTTACGCT GGGAATCTGT 
GAGCGATCGT GCAGCCAAGA CAGCATGCTA AGGTTTCCCC AGCTCTACAA GATCACACAG 
AATGCCGATG GGTTTAACAC ACGGGTTTTC TGGGGTCACT GCATCAACGC CTTGATCCAC 
TCCATAATTC TCTTCTGGTT TCCACTGAAA ATGCTCGAAC ACGATGCTGT GTTTACCAAT 
GGACAGGTTA CAGACTATCT ATTTGTTGGA AACATTGTTT ACCCTTACGT GTTAGTGACA 
GTCTGTTTGA AAGTTGGTTT GGAGACCCCG GCATGGACAA AGTTCAGTCA CCTGGCAGTT 
TGGGGCAGCA TGCTGATGTG GCTGGTATTT TTTGGAGCCT ATTCTGCGAT CTGGCCAACT 
ATTCCCATTG CTCCTGATAT GCTTGGACAG GCCGGGATGG TATTAACGTC GGGGTACTTC 
TGGCTCGGGT TACTCTTGGT CCCCACTGCA TGTCTACTAA GGGATGTGAC CTGGAGAGCG 
GCAAAGCACA CCTACCACAA GACCTTGCTG GAACAAGTGC AAGAGATTGA GACCAGAGCC 
AAAGAAATGA GTAAAGCTGC CATGCGGGAT TCCAATGGCA AAAGCTTGAA CGAACGTGAC 
CACTTGTTGA GGAGACTGGG CAGAAAAACC CCTCCGAGCC TCTTTCGTGC TAATTCCGTG 
CAACAAAGTG TATCGTACGG CTATGCATTT TCCCAAGAAG AACACGGTGT TGTTTCCCAG 
TCTCAAGTTG TCCGATCCTA TGACACAACA AAACAGAGAG CCGGGATAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813389.1
CDS	73..3666
Translation	MSASSLPLPSPPAPRPSPPSPLRFPLSDRCVLPLAGPLRCSSGYKKADDEMSGATSSADLGDSSGRCILINQPQSCKFRDNTVSTAKYSVLTFLPRFLYEQIRRAANAFFLFIALLQQIPDVSPTGRYTTLVPLIFILTVAGIKEIIEDYKRHKADNTVNKKKTIVLKGGTWQNMVWREVAVGDIVKVTNGQHLPADMIILSSSEPQAMCYIETSNLDGETNLKIRQGLPATSNLLSEEDLVKMTGRIECEGPNRHLYDFIGNLRLGTNNPVPVGPEQILLRGAQIRNTQWVIGIVVYTGHDTKLMQNSTKAPLKRSNVEKVTNVQILVLFGILLVMALVSSVGALLWNWKYEGTNWYLGDIDAVSTNFGYNLLTFIILYNNLIPISLLVTLEVVKFTQALFINWDRDMYYSETDTPAMARTSNLNEELGQVKYLFSDKTGTLTCNVMHFKKCSIGGITYGHFPEFERERSSEDFSQLPTTSESCDFDDPKLFQNIESDSPTGDTIKEFLTLLAVCHTVVPEKDEDTIIYQASSPDEGALVKGARKLGYTFTGRTPDSVIIDAMGKEQSFELLNVLEFSSNRKRMSVIVRTPSGNLRLYCKGADNVIFERLSRESKYMEQTLNHLEQFASEGLRTLCLAYCDLQDSEYQEWLNVHSEASVVIKDRAQKLEECYEIIEKDLMLLGATAIEDRLQGGVPETIATLMKAEIKIWVLTGDKQETAVNIGYSCRLVSQTMSLIVVNEDSLDATRSTLTNHCTNLGDGLGKENDLALIIDGQTLKYALSFEVRQSFLDLALSCKAVICCRVSPLQKSEIVDMVKKHVNAITLAIGDGANDVGMIQTAHVGVGISGNEGMQATNNSDYAIAQFCYLEKLLLVHGAWSYNRVTKCILYCFYKNVVLYIIELWFAVVNGFSGQVLFERWCIGLYNVIFTALPPFTLGICERSCSQDSMLRFPQLYKITQNADGFNTRVFWGHCINALIHSIILFWFPLKMLEHDAVFTNGQVTDYLFVGNIVYPYVLVTVCLKVGLETPAWTKFSHLAVWGSMLMWLVFFGAYSAIWPTIPIAPDMLGQAGMVLTSGYFWLGLLLVPTACLLRDVTWRAAKHTYHKTLLEQVQEIETRAKEMSKAAMRDSNGKSLNERDHLLRRLGRKTPPSLFRANSVQQSVSYGYAFSQEEHGVVSQSQVVRSYDTTKQRAGIE

Target ORF information:

RefSeq Version	XM_012957935.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ATPase, aminophospholipid transporter, class I, type 8A, member 2 (atp8a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957935.2

ORF Insert Sequence:

ATGTCTGCCT CCTCCCTCCC CCTGCCTTCA CCTCCAGCTC CCCGCCCCTC TCCCCCCTCC 
CCTCTCCGCT TCCCTTTATC TGATCGCTGT GTGCTCCCGC TGGCAGGTCC GCTCCGCTGC 
TCCTCGGGTT ACAAGAAGGC GGATGATGAG ATGTCCGGGG CCACGTCCAG TGCTGACTTG 
GGGGACTCCT CCGGCCGCTG CATCCTCATC AACCAACCCC AGAGCTGCAA GTTCAGGGAC 
AACACAGTCA GCACAGCCAA GTACAGTGTG TTAACGTTTC TACCTCGGTT CCTCTATGAG 
CAGATCAGGA GAGCTGCCAA TGCTTTCTTC CTCTTCATTG CCTTATTACA GCAAATTCCT 
GATGTGTCTC CGACAGGAAG ATATACCACT TTGGTGCCCT TAATCTTTAT TCTCACAGTG 
GCCGGCATCA AGGAAATCAT AGAAGACTAT AAAAGACACA AGGCAGATAA CACTGTTAAT 
AAAAAGAAAA CCATAGTGCT GAAAGGGGGG ACATGGCAGA ACATGGTTTG GAGAGAGGTG 
GCAGTAGGAG ACATTGTGAA GGTTACCAAT GGGCAACATC TTCCAGCTGA TATGATCATT 
TTATCTTCCA GTGAACCACA AGCTATGTGC TACATCGAAA CATCCAATCT TGATGGGGAA 
ACGAACTTAA AAATACGACA GGGGTTACCA GCAACATCAA ACCTTCTGTC AGAGGAGGAC 
TTGGTGAAAA TGACTGGAAG AATCGAATGT GAAGGACCTA ATCGACATCT TTATGACTTT 
ATTGGAAACC TGCGCCTAGG GACCAACAAC CCTGTTCCTG TTGGACCTGA ACAAATTTTG 
TTGAGAGGTG CCCAGATCAG AAACACCCAG TGGGTTATTG GAATAGTTGT GTACACTGGA 
CATGACACCA AATTAATGCA GAATTCAACT AAAGCGCCGC TGAAGAGATC AAATGTTGAA 
AAAGTGACCA ATGTGCAAAT CCTGGTTTTA TTTGGCATTC TCCTGGTTAT GGCTCTTGTC 
AGCTCAGTTG GAGCCTTATT ATGGAACTGG AAATATGAGG GAACCAACTG GTACCTAGGA 
GATATTGATG CAGTCTCTAC AAATTTTGGT TACAACCTTT TGACCTTCAT CATTTTGTAC 
AACAATCTCA TTCCTATCAG CCTTCTGGTG ACTTTGGAAG TAGTTAAGTT TACCCAGGCT 
CTTTTCATCA ACTGGGACAG AGATATGTAT TATTCAGAAA CAGACACCCC TGCCATGGCA 
AGAACATCTA ATCTGAATGA GGAGCTTGGA CAGGTGAAAT ATCTTTTCTC TGACAAAACT 
GGGACTTTAA CATGCAACGT CATGCACTTT AAGAAATGCA GCATTGGTGG AATTACCTAT 
GGGCACTTTC CAGAATTTGA AAGGGAACGT TCATCTGAAG ATTTCAGCCA GCTTCCAACC 
ACTAGCGAGT CCTGTGATTT TGATGACCCC AAGCTGTTCC AGAACATTGA AAGTGATTCT 
CCAACAGGAG ATACTATTAA AGAATTTCTC ACCTTGTTGG CTGTCTGTCA TACTGTTGTC 
CCCGAGAAAG ATGAAGATAC AATAATCTAC CAGGCCTCCT CTCCAGATGA AGGAGCACTA 
GTGAAAGGTG CAAGAAAACT TGGCTACACT TTTACTGGGA GAACTCCGGA CTCTGTGATT 
ATTGATGCAA TGGGGAAAGA ACAGAGCTTT GAACTCCTCA ATGTGCTGGA ATTCTCCAGC 
AACAGGAAGA GGATGTCTGT TATTGTCCGA ACTCCATCAG GGAACCTTCG CCTTTATTGC 
AAAGGAGCAG ATAATGTTAT TTTTGAGAGG CTTTCAAGAG AGTCGAAGTA TATGGAACAA 
ACCCTAAACC ACCTGGAACA ATTTGCTTCT GAAGGCTTAA GGACCTTGTG TCTTGCATAC 
TGTGACCTGC AAGATTCGGA GTATCAGGAA TGGTTGAACG TTCATAGTGA AGCCAGCGTG 
GTTATTAAGG ACAGAGCCCA AAAACTTGAA GAGTGCTATG AAATTATTGA AAAGGATTTG 
ATGCTGCTTG GTGCAACCGC CATTGAAGAC CGCCTTCAAG GAGGAGTCCC AGAGACCATA 
GCAACATTAA TGAAAGCTGA AATAAAAATA TGGGTCTTAA CAGGAGATAA ACAAGAAACA 
GCAGTCAACA TAGGTTATTC GTGCAGGCTG GTGTCACAAA CAATGTCGCT CATCGTAGTG 
AATGAGGATT CTCTGGATGC AACAAGGTCA ACACTGACTA ATCACTGTAC AAACCTTGGC 
GATGGGCTTG GCAAAGAAAA CGACCTTGCT CTAATCATCG ACGGGCAAAC GCTAAAATAC 
GCGCTTTCAT TTGAGGTTCG TCAGAGCTTT CTCGATCTGG CGCTTTCCTG CAAAGCTGTC 
ATTTGCTGCA GAGTGTCTCC GCTGCAGAAA TCTGAAATAG TAGACATGGT AAAGAAACAT 
GTGAATGCCA TCACACTGGC TATTGGAGAT GGTGCCAACG ATGTAGGAAT GATCCAGACC 
GCCCACGTAG GAGTCGGGAT CAGTGGGAAC GAGGGCATGC AAGCAACCAA CAACTCCGAT 
TACGCCATAG CGCAGTTTTG CTATTTGGAG AAGCTGCTAC TCGTGCACGG AGCTTGGAGC 
TACAATCGAG TGACCAAGTG CATCCTATAC TGTTTTTACA AGAATGTGGT TCTCTACATC 
ATCGAGCTCT GGTTTGCAGT CGTTAATGGA TTTTCTGGAC AAGTTTTGTT TGAGCGATGG 
TGCATTGGTT TGTACAATGT GATATTCACC GCCTTACCGC CTTTTACGCT GGGAATCTGT 
GAGCGATCGT GCAGCCAAGA CAGCATGCTA AGGTTTCCCC AGCTCTACAA GATCACACAG 
AATGCCGATG GGTTTAACAC ACGGGTTTTC TGGGGTCACT GCATCAACGC CTTGATCCAC 
TCCATAATTC TCTTCTGGTT TCCACTGAAA ATGCTCGAAC ACGATGCTGT GTTTACCAAT 
GGACAGGTTA CAGACTATCT ATTTGTTGGA AACATTGTTT ACCCTTACGT GTTAGTGACA 
GTCTGTTTGA AAGTTGGTTT GGAGACCCCG GCATGGACAA AGTTCAGTCA CCTGGCAGTT 
TGGGGCAGCA TGCTGATGTG GCTGGTATTT TTTGGAGCCT ATTCTGCGAT CTGGCCAACT 
ATTCCCATTG CTCCTGATAT GCTTGGACAG GCCGGGATGG TATTAACGTC GGGGTACTTC 
TGGCTCGGGT TACTCTTGGT CCCCACTGCA TGTCTACTAA GGGATGTGAC CTGGAGAGCG 
GCAAAGCACA CCTACCACAA GACCTTGCTG GAACAAGTGC AAGAGATTGA GACCAGAGCC 
AAAGAAATGA GTAAAGCTGC CATGCGGGAT TCCAATGGCA AAAGCTTGAA CGAACGTGAC 
CACTTGTTGA GGAGACTGGG CAGAAAAACC CCTCCGAGCC TCTTTCGTGC TAATTCCGTG 
CAACAAAGTG TATCGTACGG CTATGCATTT TCCCAAGAAG AACACGGTGT TGTTTCCCAG 
TCTCAAGTTG TCCGATCCTA TGACACAACA AAACAGAGAG CCGGGATAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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atp8a2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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