mylk cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following mylk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mylk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa67272 XM_031893655.1
Latest version!
Xenopus tropicalis myosin light chain kinase (mylk), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa67273 XM_031893656.1
Latest version!
Xenopus tropicalis myosin light chain kinase (mylk), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa67274 XM_031893658.1
Latest version!
Xenopus tropicalis myosin light chain kinase (mylk), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa67275 XM_031893659.1
Latest version!
Xenopus tropicalis myosin light chain kinase (mylk), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa67272
    Clone ID Related Accession (Same CDS sequence) XM_031893655.1
    Accession Version XM_031893655.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5910bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product myosin light chain kinase, smooth muscle isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTTTTTAT CGAAGGATAA AACCAGCGCC AAAGAACCTC CTTATCTTGG GGTGTCGGTT 
    CTAGACTCCA AGAACAAGAG CGTTCCACCA AAAGGCCCCG GAGACATCAT CCAAAAAGAG
    CCACTTGGAC GGACCCCGCG GGAACCCACC CAGGAGTTTA TAAGAGATTC CCCATCTGAT
    GGTTTCACAA AAGAGACGTC TTCCAAAAAG TTGCCTTCTG TAATGAACAG AGAGACCTCG
    TTCTTGTCGG CACGGGAGCG GATAATGGGG CCCACTATAA AGTCGCCTTA CGGTTCTGCT
    AAGACTTTAG GGAACCCGGC TTCTCTGTAT GAAATGTCTA AAGAGACCCG TTCAGATGTC
    CCTTCTAGAC AGATATCGAA CGTGACCCTC AGCGAGAGCT TGAAGCCAAA AGAGACCGCA
    GAATTTAGTT CTACGCAGTC TTTTCCCAAA TCTAGAGAAT CTCAATCTAG ACTCCATGGT
    ACTACAAGCA GCACCCTGTT GGGATCGAGC ACCCAGCAGC ACCCAGATAC CCCACAGCGT
    ACAGCCGAGA CCTCGCCAGA TGGTGGGGAG GTAAAACGCC AGAAGACCGC CTCCAGTACC
    ATCACCCTGA AGTCTGTCAA GATAGAAACC CAACCCAAGT CTGAGATAAG TCATGGTGGG
    ACCAGGCACA ACCAGCAGTC ACTTCCCTTG GGCCGGGAGG GGTCTCATTC ACACCCGGGG
    TTGTCGGACA CAAAAGAAAC CTTGAGCAGA AGCCTTGGAA CTGCCCCCGG CTTCGACCTG
    AGTCCCTCAA GCCAAGAGGT GCCGGAGGGC AGCGAAGTGA TATTCAGGTG CAAAGTTTCG
    GGAGATCCCA GACCGCGGGT CGAGTGGTTC CGAGAGAATT CGGCGCTGGG GGAGAGGGAC
    GGGGTGCGGA TCAGGGAAGG GGCTCGGATT CACTCGCTGC ACTTGCTGAA GGCACAGACG
    GGAGACAGCG GGAATTACAG CTGTGAAGTC TCCAACGCGA GAGGGCGGGT GTCTCATAGC
    TGGACCCTGA GCGTCACGCG GCGCAAGATG GAGGCGGTCG CGCCTCGGTT TGTCCGCAAG
    CTACGGGGCT GCTCCATTAT AGAGGGGGAG GATTTTGTGT TGGAATGTTC CATTGAGGGG
    CAACCGGCTC CTGAGATCTC GTGGCTGCTG AACGACCAGC CCATACAATA TGCCCACTCA
    GCGTATGAAG ACGGGGTGGC GAGGCTGCGG GTACAGGATG CCCTGCCGGA GGATGAGGGG
    GTATATACCT GCCTGGCACA GAACCGCGGC GGGAAGGTGT CCTGTTGTGC AACCGTCACT
    GTGACCGAAA AGAAGGCGGC AGAGGAGCCG GGGGGCGACC GAGAGCCAAA GAGCAGCCCC
    CCAATATTCC TGAAGCGCCT CACTGACCTG CAGGTTATGG ATGGGAGTCA AGTCACCATG
    AGCGTTGAAC TGTCAGGGAC CCCCCCTGCC GAGATCCTTT GGTTCCATAA TGGGAAAGAG
    ATTCAGGAGA CGGAGGATTT CCATTTTGAG CAGAGTGGTA ACGAGCACCG TCTGTGCATC
    CAGGAAGTCT TCCCCGAGGA CACAGGAACC TACATGTGCA AAGCCTGGAA TAGTTTGGGC
    CAAACTGCAA CGGAGGCCAC CTTAACCGTA CAAGAGCCGC AGGAGGGAAT CCAGCCATGG
    TTTATCAGCA AACCAAAGTG CACCAGCGCT TCAGTGGGGC AGAATGTGAT CCTATCCTGC
    GCCATCGCCG GGGACCCCTT CCCGCGGGTA CAATGGCTGA AGGACGGGCG CGAGTTGCCC
    CCCGAGCGGA TCCTGCAGAG CGACGACGTC TTCACTCTCA TCCTCAGAAA CGTCCAGCAG
    AGCGACGGCG GCCAATACCA GATCACGCTG ATCAATGCGG TGGGAGAATG CAGCTGCCGG
    GTCTCGCTGA TGGTCGAGGG AAGCTCCGGG GGTCCCTCTC ATACACACCA AACCTCCCTG
    ATTGTCTCTT TCTGTCTCTT TCCCAATCGG ACTCTCAGGC AGAGGGAGCA GCTGCGCTCT
    TGCGAGAGGG ATGGATGGGA TGAGGAGGAT GCTCTGAGTC TTGGCAGGAC TACTGAGGAG
    CAACAGGAGA ACATTCGAGG GGTTCTCAAG AGGAGAGTTG AGGTCAAGGA GAGCTCGGAG
    GAGAAGCTCC GGCAGCAAGA GGCGGAACAA GTCGACTTCC GTAGGGTTCT GGGCAAAAAG
    GTGAGCACGA GGAGCGTTTC CGAGGAGGAT TTGAAGGACA TCCCACCGGA GCAGATGGAC
    TTCCGAGCCA ACCTCCAGCG GCAGGTCAAA CCTAAGGCTG AGGATGAACG CAAGGTCAAT
    AGCCCACAGC AGGTGGACTT CCGCTCGGTA CTGGGCAAGA AGCCATCGAC TCCCAAAACC
    CCTCTTCCAG AGAAGGTGGT CGCCAAGGCA GCAACTCCTG ACTTCAGGTC GGTCCTCGGT
    GGCAAGAAAA AGCTTCCCAC TGAGAATGGC AGCCCCAACG ACGTTCCACA TGAACAGGGC
    AAATCAAAGG TGAACTGCTG CACGGTAGTG GATGGGGGGG TTGCCAAGAA CAGCGTGACC
    AACAACAGTG TCCCTAATGC AGCCAAGATC AGTGTCCTCA GTGTGGACAA GGCCAATGTC
    CCAGGTACAG CCAAGAACAG TGTCCCCAGT GTGGACAAGA CCAGTGCCCC AGGTACAGCC
    AAGAACAGTG TCCCCAGTGT GGACAAGACC AGTGCCCCAA GTACAGCCAA GAACAGTGTC
    TCAAGTGGAT CCAAGGCCAG TTTCCTAGGT GCGGATAAGG CCGGTGTCCC AAGTGTGGCC
    AAGACCAGTG TCCCAACTGC AACCAAGACC AATGTCCCAA GTGTGGACAA GACTAATGTC
    CCTGCCGCAA CCAAGACCAA TGTCCCAAGT TTGGCCAAGA CCAGTCTTCC AACTGCAACC
    AAGACCAATG TCCCAAGTGT GGACAAGGCC AGTGTCCCAA GTGTGGACAA GTCTAGTGTC
    CCTGCTGCAG TCAAGACCAA TGATAAGGAC GTTGCCCCAA GTGCAGACAA GCAGCTAACC
    CCAAATGTGG ATAAAGATAT TGCCCCAAGT TCAGAAAAGG ACGTTGCCCC AAGTGTGGAC
    AAGGACGTTG CCCCAAGTTC AGAGAAGGAC GTTGCCCCAA GTGTGGACAA GGACATTGCC
    CCAAGTGTGG ACAAGGACAT TGCCCCAAGT TCAGACAAAG ACGTTGCCCC CAGTTCAGAC
    AAGGATGTTG CCCCCAGTTC AGACAAGGAT GTTGCCCCCG GTTCAGACAA GGATGTTGCC
    CCCAGTTCAG ACAAGGATGT TGCCCCCAGT TCAGAGAAGG ACGTTGCCCC GAGTTCAGAG
    AAGGACGTTG CCCCAAATTC AGAGAAGGAC GTTGCCCCAA ATTCAGAGAA GGACGTTGCC
    CCAAGTTCAG AAAAGGACGT TGCCCCAAGT GCGCCCACAA AACCCGAGTT TTCGGAGGTA
    CTGCAGGATG TACGTGTTAC CGATGGGGAG AAGCTGGTGT TGCAGTGCCG CGTCACAGCC
    GAACCACCTC CGACCGTTGT GTGGACTCTG GATGGAAAAG TCATCAAGTC CTCCAAGTAC
    ATTGTCCTGT CCCAAGAAGG CTCCGTGTGT TCCCTGAGCA TCGAGAAGAC CTTCCCGGAG
    GACGAGGGGC AGTACCTGTG CGCGGTGGAG AATGCGGCCG GGAGGGCAGA ATGTGCCTGT
    AATGTCACGG TGGAAGATAC CTCGGGCAAC AAGGGCATCA AGAAGAGCAA GAAGCCGAGA
    AGCAACGCTC CGCTCTCCAA CGCAACGGAA AATGAAGCTA CGATTAAGAA GAAGCCGGCT
    CCGAAGACCC CCCCCAAAGC AGCATCTGCC CCTCAGATCC TACAGTTCCC AGAAGACAGG
    AAGGTGAAAG CCGGGGAGTC GGTCGAGCTC CTGTGCAAGG TGGGGGGGAC CCAGCCCATT
    ACCTGCATGT GGATGAAGTT CAGGAAGGAG ATTAAAGAAA GTGAATTTAT CAAGATTGAG
    AGCACAGAAG AGACCAGTAA GCTGACAATC TCCTCGGCCC GACAGGAGCA CTGCGGCTGC
    TACACCCTGG TGGCGGAGAA CAAACAGGGC AGCAAACAGG CGCAGGTTAA CCTGACTGTG
    TTTGATAAAC CCGACCCGCC GGCGGGGGTG CCCTGTGCCT CCTGTATGAA GGGCTCGGTG
    CTCACCCTGT CGTGGTACGG CCCCACGTAT GATGGGGGCA GTGTGGTCGA GTCCTACTCG
    GTGGAGATCT GGGACTCAGT GGGCAAAGTC TGGACGGATC TGACCTCCTG CCGCAGCACC
    TCCTTTAATG TGGAGAATTT ACTGCAGGAC AGAGAGTACA AATTCCGGGT CCGGGCCACT
    AACATCCACG GCACCAGCGA ACCCAGTCAG GAGTCGGAAC CAGTTACCTA CAAGGAGAAG
    GAAGAAGAAC ACAAAGAAGA AGAGGCAGAA GTTTCAGACG ACGAGGAGAA GGAGTCGGAG
    CCGGAATACA GGGACGTCAC TCTCAACAGC AGCGAGAAAG TGACCGACTA CTACGATGTG
    GAGGAGAGAC TGGGCACGGG CAAATTTGGC CAAGTCTTCC GAATCGTAGA GAAGAAGAGC
    AGGAAAATCT ATGCCGGGAA GTTCTTCAAA GCCTATTCGG CCAAAGACAA GGAGGGCATC
    CGGCAAGAAA TTGCCATCAT GAACTGCCTG CACCACCCCA AGCTGGTCCA GTGCGTCGAC
    GCGTTTGAGT CCAAAGCCGA CATGGTGATG GTTCTGGAAC TGGTCTCGGG CGGGGAACTC
    TTCGAGCGAA TCATTGACGA GGATTTTGAG CTGACGGAGC GGGAGGTGAT TAAGTACATG
    AAGCAGATCT GCGAGGGGGT CGAGTTCATC CACAAACAGG GGATCGTGCA CCTCGACCTC
    AAACCCGAGA ACATCATGTG CGTCAATAAG ACTGGCACCA AGATCAAACT CATCGACTTC
    GGCCTCGCCC GCAGATTGGA GAGCTGCCCG AACCTCAAAG TGCTTTTTGG AACCCCTGAA
    TTTGTAGCCC CCGAAGTGAT AAACTACGAG CCGATTGGTT ACTCGACGGA CATGTGGAGC
    ATCGGGGTCA TCTGCTACAT CTTGGTCAGT GGACTTTCTC CATTCATGGG GGATAATGAC
    AATGAAACGC TGGCGAATGT AACTTCGGCC ACTTGGGACT TCGATGATGA AGCCTTTGAT
    GAAATATCTG AAGAAGCCAA AAACTTAATC AGCAGTTTGT TGAAGAAGGA CATGAAGAAT
    CGGCTGAACT GTACCCAGTG CCTCCAGCAT TCGTGGCTAC AGCAGGACAC TGCCACAATG
    GAAGCCAAGA AACTCTCCAA AGACAGGATG AAGAAATATA TGGCTCGGAG GAAATGGCAG
    AAAACCGGGC ACGCAGTGAG GGCAATCGGA CGTCTCTCCT CTATGGCCAT GATCTCTGGG
    CTGAGCGCCA GGAAGTCATC GGGCAGCGCC CCCAGCAGCC CCCTGTCTGC GGAGCTTGAA
    GCCAATGAAG AAGACAACCA GGCTTTCCTG GAGTCGGTGA CAGAGGAGAA GCCCCCCGCA
    AAGCCTTTCT TCACGAAGAC CATTCTGGAT ATCGAAGTCG TGGAAGGGAG CGCCGCGAGG
    TTTGACTGCA GAATTGAGGG GCACCCCGAC CCGGAGGTGA TTTGGTACAA GGACGACCAA
    CCCATCAAGG AGTCTCGGCA CTTCCAGATC GATTACGACG AGGACGGTAA CTGCTCCCTG
    ACCATCTCGG AAGTTTGCGG CGACGACGAC GCCAAATACA CCTGCAAAGC CGCCAACAGC
    CTGGGGGATA CGACCTGCAC GGCCGAGCTG ATTGTAGAGG TGATGGCGGA GGAGGAGGAG
    GAAGAGGAGG AGGAAGAGGA GGAGGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031749515.1
    CDS516..6425
    Translation

    Target ORF information:

    RefSeq Version XM_031893655.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis myosin light chain kinase (mylk), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031893655.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTTTTTAT CGAAGGATAA AACCAGCGCC AAAGAACCTC CTTATCTTGG GGTGTCGGTT 
    CTAGACTCCA AGAACAAGAG CGTTCCACCA AAAGGCCCCG GAGACATCAT CCAAAAAGAG
    CCACTTGGAC GGACCCCGCG GGAACCCACC CAGGAGTTTA TAAGAGATTC CCCATCTGAT
    GGTTTCACAA AAGAGACGTC TTCCAAAAAG TTGCCTTCTG TAATGAACAG AGAGACCTCG
    TTCTTGTCGG CACGGGAGCG GATAATGGGG CCCACTATAA AGTCGCCTTA CGGTTCTGCT
    AAGACTTTAG GGAACCCGGC TTCTCTGTAT GAAATGTCTA AAGAGACCCG TTCAGATGTC
    CCTTCTAGAC AGATATCGAA CGTGACCCTC AGCGAGAGCT TGAAGCCAAA AGAGACCGCA
    GAATTTAGTT CTACGCAGTC TTTTCCCAAA TCTAGAGAAT CTCAATCTAG ACTCCATGGT
    ACTACAAGCA GCACCCTGTT GGGATCGAGC ACCCAGCAGC ACCCAGATAC CCCACAGCGT
    ACAGCCGAGA CCTCGCCAGA TGGTGGGGAG GTAAAACGCC AGAAGACCGC CTCCAGTACC
    ATCACCCTGA AGTCTGTCAA GATAGAAACC CAACCCAAGT CTGAGATAAG TCATGGTGGG
    ACCAGGCACA ACCAGCAGTC ACTTCCCTTG GGCCGGGAGG GGTCTCATTC ACACCCGGGG
    TTGTCGGACA CAAAAGAAAC CTTGAGCAGA AGCCTTGGAA CTGCCCCCGG CTTCGACCTG
    AGTCCCTCAA GCCAAGAGGT GCCGGAGGGC AGCGAAGTGA TATTCAGGTG CAAAGTTTCG
    GGAGATCCCA GACCGCGGGT CGAGTGGTTC CGAGAGAATT CGGCGCTGGG GGAGAGGGAC
    GGGGTGCGGA TCAGGGAAGG GGCTCGGATT CACTCGCTGC ACTTGCTGAA GGCACAGACG
    GGAGACAGCG GGAATTACAG CTGTGAAGTC TCCAACGCGA GAGGGCGGGT GTCTCATAGC
    TGGACCCTGA GCGTCACGCG GCGCAAGATG GAGGCGGTCG CGCCTCGGTT TGTCCGCAAG
    CTACGGGGCT GCTCCATTAT AGAGGGGGAG GATTTTGTGT TGGAATGTTC CATTGAGGGG
    CAACCGGCTC CTGAGATCTC GTGGCTGCTG AACGACCAGC CCATACAATA TGCCCACTCA
    GCGTATGAAG ACGGGGTGGC GAGGCTGCGG GTACAGGATG CCCTGCCGGA GGATGAGGGG
    GTATATACCT GCCTGGCACA GAACCGCGGC GGGAAGGTGT CCTGTTGTGC AACCGTCACT
    GTGACCGAAA AGAAGGCGGC AGAGGAGCCG GGGGGCGACC GAGAGCCAAA GAGCAGCCCC
    CCAATATTCC TGAAGCGCCT CACTGACCTG CAGGTTATGG ATGGGAGTCA AGTCACCATG
    AGCGTTGAAC TGTCAGGGAC CCCCCCTGCC GAGATCCTTT GGTTCCATAA TGGGAAAGAG
    ATTCAGGAGA CGGAGGATTT CCATTTTGAG CAGAGTGGTA ACGAGCACCG TCTGTGCATC
    CAGGAAGTCT TCCCCGAGGA CACAGGAACC TACATGTGCA AAGCCTGGAA TAGTTTGGGC
    CAAACTGCAA CGGAGGCCAC CTTAACCGTA CAAGAGCCGC AGGAGGGAAT CCAGCCATGG
    TTTATCAGCA AACCAAAGTG CACCAGCGCT TCAGTGGGGC AGAATGTGAT CCTATCCTGC
    GCCATCGCCG GGGACCCCTT CCCGCGGGTA CAATGGCTGA AGGACGGGCG CGAGTTGCCC
    CCCGAGCGGA TCCTGCAGAG CGACGACGTC TTCACTCTCA TCCTCAGAAA CGTCCAGCAG
    AGCGACGGCG GCCAATACCA GATCACGCTG ATCAATGCGG TGGGAGAATG CAGCTGCCGG
    GTCTCGCTGA TGGTCGAGGG AAGCTCCGGG GGTCCCTCTC ATACACACCA AACCTCCCTG
    ATTGTCTCTT TCTGTCTCTT TCCCAATCGG ACTCTCAGGC AGAGGGAGCA GCTGCGCTCT
    TGCGAGAGGG ATGGATGGGA TGAGGAGGAT GCTCTGAGTC TTGGCAGGAC TACTGAGGAG
    CAACAGGAGA ACATTCGAGG GGTTCTCAAG AGGAGAGTTG AGGTCAAGGA GAGCTCGGAG
    GAGAAGCTCC GGCAGCAAGA GGCGGAACAA GTCGACTTCC GTAGGGTTCT GGGCAAAAAG
    GTGAGCACGA GGAGCGTTTC CGAGGAGGAT TTGAAGGACA TCCCACCGGA GCAGATGGAC
    TTCCGAGCCA ACCTCCAGCG GCAGGTCAAA CCTAAGGCTG AGGATGAACG CAAGGTCAAT
    AGCCCACAGC AGGTGGACTT CCGCTCGGTA CTGGGCAAGA AGCCATCGAC TCCCAAAACC
    CCTCTTCCAG AGAAGGTGGT CGCCAAGGCA GCAACTCCTG ACTTCAGGTC GGTCCTCGGT
    GGCAAGAAAA AGCTTCCCAC TGAGAATGGC AGCCCCAACG ACGTTCCACA TGAACAGGGC
    AAATCAAAGG TGAACTGCTG CACGGTAGTG GATGGGGGGG TTGCCAAGAA CAGCGTGACC
    AACAACAGTG TCCCTAATGC AGCCAAGATC AGTGTCCTCA GTGTGGACAA GGCCAATGTC
    CCAGGTACAG CCAAGAACAG TGTCCCCAGT GTGGACAAGA CCAGTGCCCC AGGTACAGCC
    AAGAACAGTG TCCCCAGTGT GGACAAGACC AGTGCCCCAA GTACAGCCAA GAACAGTGTC
    TCAAGTGGAT CCAAGGCCAG TTTCCTAGGT GCGGATAAGG CCGGTGTCCC AAGTGTGGCC
    AAGACCAGTG TCCCAACTGC AACCAAGACC AATGTCCCAA GTGTGGACAA GACTAATGTC
    CCTGCCGCAA CCAAGACCAA TGTCCCAAGT TTGGCCAAGA CCAGTCTTCC AACTGCAACC
    AAGACCAATG TCCCAAGTGT GGACAAGGCC AGTGTCCCAA GTGTGGACAA GTCTAGTGTC
    CCTGCTGCAG TCAAGACCAA TGATAAGGAC GTTGCCCCAA GTGCAGACAA GCAGCTAACC
    CCAAATGTGG ATAAAGATAT TGCCCCAAGT TCAGAAAAGG ACGTTGCCCC AAGTGTGGAC
    AAGGACGTTG CCCCAAGTTC AGAGAAGGAC GTTGCCCCAA GTGTGGACAA GGACATTGCC
    CCAAGTGTGG ACAAGGACAT TGCCCCAAGT TCAGACAAAG ACGTTGCCCC CAGTTCAGAC
    AAGGATGTTG CCCCCAGTTC AGACAAGGAT GTTGCCCCCG GTTCAGACAA GGATGTTGCC
    CCCAGTTCAG ACAAGGATGT TGCCCCCAGT TCAGAGAAGG ACGTTGCCCC GAGTTCAGAG
    AAGGACGTTG CCCCAAATTC AGAGAAGGAC GTTGCCCCAA ATTCAGAGAA GGACGTTGCC
    CCAAGTTCAG AAAAGGACGT TGCCCCAAGT GCGCCCACAA AACCCGAGTT TTCGGAGGTA
    CTGCAGGATG TACGTGTTAC CGATGGGGAG AAGCTGGTGT TGCAGTGCCG CGTCACAGCC
    GAACCACCTC CGACCGTTGT GTGGACTCTG GATGGAAAAG TCATCAAGTC CTCCAAGTAC
    ATTGTCCTGT CCCAAGAAGG CTCCGTGTGT TCCCTGAGCA TCGAGAAGAC CTTCCCGGAG
    GACGAGGGGC AGTACCTGTG CGCGGTGGAG AATGCGGCCG GGAGGGCAGA ATGTGCCTGT
    AATGTCACGG TGGAAGATAC CTCGGGCAAC AAGGGCATCA AGAAGAGCAA GAAGCCGAGA
    AGCAACGCTC CGCTCTCCAA CGCAACGGAA AATGAAGCTA CGATTAAGAA GAAGCCGGCT
    CCGAAGACCC CCCCCAAAGC AGCATCTGCC CCTCAGATCC TACAGTTCCC AGAAGACAGG
    AAGGTGAAAG CCGGGGAGTC GGTCGAGCTC CTGTGCAAGG TGGGGGGGAC CCAGCCCATT
    ACCTGCATGT GGATGAAGTT CAGGAAGGAG ATTAAAGAAA GTGAATTTAT CAAGATTGAG
    AGCACAGAAG AGACCAGTAA GCTGACAATC TCCTCGGCCC GACAGGAGCA CTGCGGCTGC
    TACACCCTGG TGGCGGAGAA CAAACAGGGC AGCAAACAGG CGCAGGTTAA CCTGACTGTG
    TTTGATAAAC CCGACCCGCC GGCGGGGGTG CCCTGTGCCT CCTGTATGAA GGGCTCGGTG
    CTCACCCTGT CGTGGTACGG CCCCACGTAT GATGGGGGCA GTGTGGTCGA GTCCTACTCG
    GTGGAGATCT GGGACTCAGT GGGCAAAGTC TGGACGGATC TGACCTCCTG CCGCAGCACC
    TCCTTTAATG TGGAGAATTT ACTGCAGGAC AGAGAGTACA AATTCCGGGT CCGGGCCACT
    AACATCCACG GCACCAGCGA ACCCAGTCAG GAGTCGGAAC CAGTTACCTA CAAGGAGAAG
    GAAGAAGAAC ACAAAGAAGA AGAGGCAGAA GTTTCAGACG ACGAGGAGAA GGAGTCGGAG
    CCGGAATACA GGGACGTCAC TCTCAACAGC AGCGAGAAAG TGACCGACTA CTACGATGTG
    GAGGAGAGAC TGGGCACGGG CAAATTTGGC CAAGTCTTCC GAATCGTAGA GAAGAAGAGC
    AGGAAAATCT ATGCCGGGAA GTTCTTCAAA GCCTATTCGG CCAAAGACAA GGAGGGCATC
    CGGCAAGAAA TTGCCATCAT GAACTGCCTG CACCACCCCA AGCTGGTCCA GTGCGTCGAC
    GCGTTTGAGT CCAAAGCCGA CATGGTGATG GTTCTGGAAC TGGTCTCGGG CGGGGAACTC
    TTCGAGCGAA TCATTGACGA GGATTTTGAG CTGACGGAGC GGGAGGTGAT TAAGTACATG
    AAGCAGATCT GCGAGGGGGT CGAGTTCATC CACAAACAGG GGATCGTGCA CCTCGACCTC
    AAACCCGAGA ACATCATGTG CGTCAATAAG ACTGGCACCA AGATCAAACT CATCGACTTC
    GGCCTCGCCC GCAGATTGGA GAGCTGCCCG AACCTCAAAG TGCTTTTTGG AACCCCTGAA
    TTTGTAGCCC CCGAAGTGAT AAACTACGAG CCGATTGGTT ACTCGACGGA CATGTGGAGC
    ATCGGGGTCA TCTGCTACAT CTTGGTCAGT GGACTTTCTC CATTCATGGG GGATAATGAC
    AATGAAACGC TGGCGAATGT AACTTCGGCC ACTTGGGACT TCGATGATGA AGCCTTTGAT
    GAAATATCTG AAGAAGCCAA AAACTTAATC AGCAGTTTGT TGAAGAAGGA CATGAAGAAT
    CGGCTGAACT GTACCCAGTG CCTCCAGCAT TCGTGGCTAC AGCAGGACAC TGCCACAATG
    GAAGCCAAGA AACTCTCCAA AGACAGGATG AAGAAATATA TGGCTCGGAG GAAATGGCAG
    AAAACCGGGC ACGCAGTGAG GGCAATCGGA CGTCTCTCCT CTATGGCCAT GATCTCTGGG
    CTGAGCGCCA GGAAGTCATC GGGCAGCGCC CCCAGCAGCC CCCTGTCTGC GGAGCTTGAA
    GCCAATGAAG AAGACAACCA GGCTTTCCTG GAGTCGGTGA CAGAGGAGAA GCCCCCCGCA
    AAGCCTTTCT TCACGAAGAC CATTCTGGAT ATCGAAGTCG TGGAAGGGAG CGCCGCGAGG
    TTTGACTGCA GAATTGAGGG GCACCCCGAC CCGGAGGTGA TTTGGTACAA GGACGACCAA
    CCCATCAAGG AGTCTCGGCA CTTCCAGATC GATTACGACG AGGACGGTAA CTGCTCCCTG
    ACCATCTCGG AAGTTTGCGG CGACGACGAC GCCAAATACA CCTGCAAAGC CGCCAACAGC
    CTGGGGGATA CGACCTGCAC GGCCGAGCTG ATTGTAGAGG TGATGGCGGA GGAGGAGGAG
    GAAGAGGAGG AGGAAGAGGA GGAGGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa67273
    Clone ID Related Accession (Same CDS sequence) XM_031893656.1
    Accession Version XM_031893656.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5868bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product myosin light chain kinase, smooth muscle isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGTTTTTAT CGAAGGATAA AACCAGCGCC AAAGAACCTC CTTATCTTGG GGTGTCGGTT 
    CTAGACTCCA AGAACAAGAG CGTTCCACCA AAAGGCCCCG GAGACATCAT CCAAAAAGAG
    CCACTTGGAC GGACCCCGCG GGAACCCACC CAGGAGTTTA TAAGAGATTC CCCATCTGAT
    GGTTTCACAA AAGAGACGTC TTCCAAAAAG TTGCCTTCTG TAATGAACAG AGAGACCTCG
    TTCTTGTCGG CACGGGAGCG GATAATGGGG CCCACTATAA AGTCGCCTTA CGGTTCTGCT
    AAGACTTTAG GGAACCCGGC TTCTCTGTAT GAAATGTCTA AAGAGACCCG TTCAGATGTC
    CCTTCTAGAC AGATATCGAA CGTGACCCTC AGCGAGAGCT TGAAGCCAAA AGAGACCGCA
    GAATTTAGTT CTACGCAGTC TTTTCCCAAA TCTAGAGAAT CTCAATCTAG ACTCCATGGT
    ACTACAAGCA GCACCCTGTT GGGATCGAGC ACCCAGCAGC ACCCAGATAC CCCACAGCGT
    ACAGCCGAGA CCTCGCCAGA TGGTGGGGAG GTAAAACGCC AGAAGACCGC CTCCAGTACC
    ATCACCCTGA AGTCTGTCAA GATAGAAACC CAACCCAAGT CTGAGATAAG TCATGGTGGG
    ACCAGGCACA ACCAGCAGTC ACTTCCCTTG GGCCGGGAGG GGTCTCATTC ACACCCGGGG
    TTGTCGGACA CAAAAGAAAC CTTGAGCAGA AGCCTTGGAA CTGCCCCCGG CTTCGACCTG
    AGTCCCTCAA GCCAAGAGGT GCCGGAGGGC AGCGAAGTGA TATTCAGGTG CAAAGTTTCG
    GGAGATCCCA GACCGCGGGT CGAGTGGTTC CGAGAGAATT CGGCGCTGGG GGAGAGGGAC
    GGGGTGCGGA TCAGGGAAGG GGCTCGGATT CACTCGCTGC ACTTGCTGAA GGCACAGACG
    GGAGACAGCG GGAATTACAG CTGTGAAGTC TCCAACGCGA GAGGGCGGGT GTCTCATAGC
    TGGACCCTGA GCGTCACGCG GCGCAAGATG GAGGCGGTCG CGCCTCGGTT TGTCCGCAAG
    CTACGGGGCT GCTCCATTAT AGAGGGGGAG GATTTTGTGT TGGAATGTTC CATTGAGGGG
    CAACCGGCTC CTGAGATCTC GTGGCTGCTG AACGACCAGC CCATACAATA TGCCCACTCA
    GCGTATGAAG ACGGGGTGGC GAGGCTGCGG GTACAGGATG CCCTGCCGGA GGATGAGGGG
    GTATATACCT GCCTGGCACA GAACCGCGGC GGGAAGGTGT CCTGTTGTGC AACCGTCACT
    GTGACCGAAA AGAAGGCGGC AGAGGAGCCG GGGGGCGACC GAGAGCCAAA GAGCAGCCCC
    CCAATATTCC TGAAGCGCCT CACTGACCTG CAGGTTATGG ATGGGAGTCA AGTCACCATG
    AGCGTTGAAC TGTCAGGGAC CCCCCCTGCC GAGATCCTTT GGTTCCATAA TGGGAAAGAG
    ATTCAGGAGA CGGAGGATTT CCATTTTGAG CAGAGTGGTA ACGAGCACCG TCTGTGCATC
    CAGGAAGTCT TCCCCGAGGA CACAGGAACC TACATGTGCA AAGCCTGGAA TAGTTTGGGC
    CAAACTGCAA CGGAGGCCAC CTTAACCGTA CAAGAGCCGC AGGAGGGAAT CCAGCCATGG
    TTTATCAGCA AACCAAAGTG CACCAGCGCT TCAGTGGGGC AGAATGTGAT CCTATCCTGC
    GCCATCGCCG GGGACCCCTT CCCGCGGGTA CAATGGCTGA AGGACGGGCG CGAGTTGCCC
    CCCGAGCGGA TCCTGCAGAG CGACGACGTC TTCACTCTCA TCCTCAGAAA CGTCCAGCAG
    AGCGACGGCG GCCAATACCA GATCACGCTG ATCAATGCGG TGGGAGAATG CAGCTGCCGG
    GTCTCGCTGA TGGTCGAGGG AAGCTCCGGG GGTCCCTCTC ATACACAGAC TCTCAGGCAG
    AGGGAGCAGC TGCGCTCTTG CGAGAGGGAT GGATGGGATG AGGAGGATGC TCTGAGTCTT
    GGCAGGACTA CTGAGGAGCA ACAGGAGAAC ATTCGAGGGG TTCTCAAGAG GAGAGTTGAG
    GTCAAGGAGA GCTCGGAGGA GAAGCTCCGG CAGCAAGAGG CGGAACAAGT CGACTTCCGT
    AGGGTTCTGG GCAAAAAGGT GAGCACGAGG AGCGTTTCCG AGGAGGATTT GAAGGACATC
    CCACCGGAGC AGATGGACTT CCGAGCCAAC CTCCAGCGGC AGGTCAAACC TAAGGCTGAG
    GATGAACGCA AGGTCAATAG CCCACAGCAG GTGGACTTCC GCTCGGTACT GGGCAAGAAG
    CCATCGACTC CCAAAACCCC TCTTCCAGAG AAGGTGGTCG CCAAGGCAGC AACTCCTGAC
    TTCAGGTCGG TCCTCGGTGG CAAGAAAAAG CTTCCCACTG AGAATGGCAG CCCCAACGAC
    GTTCCACATG AACAGGGCAA ATCAAAGGTG AACTGCTGCA CGGTAGTGGA TGGGGGGGTT
    GCCAAGAACA GCGTGACCAA CAACAGTGTC CCTAATGCAG CCAAGATCAG TGTCCTCAGT
    GTGGACAAGG CCAATGTCCC AGGTACAGCC AAGAACAGTG TCCCCAGTGT GGACAAGACC
    AGTGCCCCAG GTACAGCCAA GAACAGTGTC CCCAGTGTGG ACAAGACCAG TGCCCCAAGT
    ACAGCCAAGA ACAGTGTCTC AAGTGGATCC AAGGCCAGTT TCCTAGGTGC GGATAAGGCC
    GGTGTCCCAA GTGTGGCCAA GACCAGTGTC CCAACTGCAA CCAAGACCAA TGTCCCAAGT
    GTGGACAAGA CTAATGTCCC TGCCGCAACC AAGACCAATG TCCCAAGTTT GGCCAAGACC
    AGTCTTCCAA CTGCAACCAA GACCAATGTC CCAAGTGTGG ACAAGGCCAG TGTCCCAAGT
    GTGGACAAGT CTAGTGTCCC TGCTGCAGTC AAGACCAATG ATAAGGACGT TGCCCCAAGT
    GCAGACAAGC AGCTAACCCC AAATGTGGAT AAAGATATTG CCCCAAGTTC AGAAAAGGAC
    GTTGCCCCAA GTGTGGACAA GGACGTTGCC CCAAGTTCAG AGAAGGACGT TGCCCCAAGT
    GTGGACAAGG ACATTGCCCC AAGTGTGGAC AAGGACATTG CCCCAAGTTC AGACAAAGAC
    GTTGCCCCCA GTTCAGACAA GGATGTTGCC CCCAGTTCAG ACAAGGATGT TGCCCCCGGT
    TCAGACAAGG ATGTTGCCCC CAGTTCAGAC AAGGATGTTG CCCCCAGTTC AGAGAAGGAC
    GTTGCCCCGA GTTCAGAGAA GGACGTTGCC CCAAATTCAG AGAAGGACGT TGCCCCAAAT
    TCAGAGAAGG ACGTTGCCCC AAGTTCAGAA AAGGACGTTG CCCCAAGTGC GCCCACAAAA
    CCCGAGTTTT CGGAGGTACT GCAGGATGTA CGTGTTACCG ATGGGGAGAA GCTGGTGTTG
    CAGTGCCGCG TCACAGCCGA ACCACCTCCG ACCGTTGTGT GGACTCTGGA TGGAAAAGTC
    ATCAAGTCCT CCAAGTACAT TGTCCTGTCC CAAGAAGGCT CCGTGTGTTC CCTGAGCATC
    GAGAAGACCT TCCCGGAGGA CGAGGGGCAG TACCTGTGCG CGGTGGAGAA TGCGGCCGGG
    AGGGCAGAAT GTGCCTGTAA TGTCACGGTG GAAGATACCT CGGGCAACAA GGGCATCAAG
    AAGAGCAAGA AGCCGAGAAG CAACGCTCCG CTCTCCAACG CAACGGAAAA TGAAGCTACG
    ATTAAGAAGA AGCCGGCTCC GAAGACCCCC CCCAAAGCAG CATCTGCCCC TCAGATCCTA
    CAGTTCCCAG AAGACAGGAA GGTGAAAGCC GGGGAGTCGG TCGAGCTCCT GTGCAAGGTG
    GGGGGGACCC AGCCCATTAC CTGCATGTGG ATGAAGTTCA GGAAGGAGAT TAAAGAAAGT
    GAATTTATCA AGATTGAGAG CACAGAAGAG ACCAGTAAGC TGACAATCTC CTCGGCCCGA
    CAGGAGCACT GCGGCTGCTA CACCCTGGTG GCGGAGAACA AACAGGGCAG CAAACAGGCG
    CAGGTTAACC TGACTGTGTT TGATAAACCC GACCCGCCGG CGGGGGTGCC CTGTGCCTCC
    TGTATGAAGG GCTCGGTGCT CACCCTGTCG TGGTACGGCC CCACGTATGA TGGGGGCAGT
    GTGGTCGAGT CCTACTCGGT GGAGATCTGG GACTCAGTGG GCAAAGTCTG GACGGATCTG
    ACCTCCTGCC GCAGCACCTC CTTTAATGTG GAGAATTTAC TGCAGGACAG AGAGTACAAA
    TTCCGGGTCC GGGCCACTAA CATCCACGGC ACCAGCGAAC CCAGTCAGGA GTCGGAACCA
    GTTACCTACA AGGAGAAGGA AGAAGAACAC AAAGAAGAAG AGGCAGAAGT TTCAGACGAC
    GAGGAGAAGG AGTCGGAGCC GGAATACAGG GACGTCACTC TCAACAGCAG CGAGAAAGTG
    ACCGACTACT ACGATGTGGA GGAGAGACTG GGCACGGGCA AATTTGGCCA AGTCTTCCGA
    ATCGTAGAGA AGAAGAGCAG GAAAATCTAT GCCGGGAAGT TCTTCAAAGC CTATTCGGCC
    AAAGACAAGG AGGGCATCCG GCAAGAAATT GCCATCATGA ACTGCCTGCA CCACCCCAAG
    CTGGTCCAGT GCGTCGACGC GTTTGAGTCC AAAGCCGACA TGGTGATGGT TCTGGAACTG
    GTCTCGGGCG GGGAACTCTT CGAGCGAATC ATTGACGAGG ATTTTGAGCT GACGGAGCGG
    GAGGTGATTA AGTACATGAA GCAGATCTGC GAGGGGGTCG AGTTCATCCA CAAACAGGGG
    ATCGTGCACC TCGACCTCAA ACCCGAGAAC ATCATGTGCG TCAATAAGAC TGGCACCAAG
    ATCAAACTCA TCGACTTCGG CCTCGCCCGC AGATTGGAGA GCTGCCCGAA CCTCAAAGTG
    CTTTTTGGAA CCCCTGAATT TGTAGCCCCC GAAGTGATAA ACTACGAGCC GATTGGTTAC
    TCGACGGACA TGTGGAGCAT CGGGGTCATC TGCTACATCT TGGTCAGTGG ACTTTCTCCA
    TTCATGGGGG ATAATGACAA TGAAACGCTG GCGAATGTAA CTTCGGCCAC TTGGGACTTC
    GATGATGAAG CCTTTGATGA AATATCTGAA GAAGCCAAAA ACTTAATCAG CAGTTTGTTG
    AAGAAGGACA TGAAGAATCG GCTGAACTGT ACCCAGTGCC TCCAGCATTC GTGGCTACAG
    CAGGACACTG CCACAATGGA AGCCAAGAAA CTCTCCAAAG ACAGGATGAA GAAATATATG
    GCTCGGAGGA AATGGCAGAA AACCGGGCAC GCAGTGAGGG CAATCGGACG TCTCTCCTCT
    ATGGCCATGA TCTCTGGGCT GAGCGCCAGG AAGTCATCGG GCAGCGCCCC CAGCAGCCCC
    CTGTCTGCGG AGCTTGAAGC CAATGAAGAA GACAACCAGG CTTTCCTGGA GTCGGTGACA
    GAGGAGAAGC CCCCCGCAAA GCCTTTCTTC ACGAAGACCA TTCTGGATAT CGAAGTCGTG
    GAAGGGAGCG CCGCGAGGTT TGACTGCAGA ATTGAGGGGC ACCCCGACCC GGAGGTGATT
    TGGTACAAGG ACGACCAACC CATCAAGGAG TCTCGGCACT TCCAGATCGA TTACGACGAG
    GACGGTAACT GCTCCCTGAC CATCTCGGAA GTTTGCGGCG ACGACGACGC CAAATACACC
    TGCAAAGCCG CCAACAGCCT GGGGGATACG ACCTGCACGG CCGAGCTGAT TGTAGAGGTG
    ATGGCGGAGG AGGAGGAGGA AGAGGAGGAG GAAGAGGAGG AGGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031749516.1
    CDS516..6383
    Translation

    Target ORF information:

    RefSeq Version XM_031893656.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis myosin light chain kinase (mylk), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031893656.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGTTTTTAT CGAAGGATAA AACCAGCGCC AAAGAACCTC CTTATCTTGG GGTGTCGGTT 
    CTAGACTCCA AGAACAAGAG CGTTCCACCA AAAGGCCCCG GAGACATCAT CCAAAAAGAG
    CCACTTGGAC GGACCCCGCG GGAACCCACC CAGGAGTTTA TAAGAGATTC CCCATCTGAT
    GGTTTCACAA AAGAGACGTC TTCCAAAAAG TTGCCTTCTG TAATGAACAG AGAGACCTCG
    TTCTTGTCGG CACGGGAGCG GATAATGGGG CCCACTATAA AGTCGCCTTA CGGTTCTGCT
    AAGACTTTAG GGAACCCGGC TTCTCTGTAT GAAATGTCTA AAGAGACCCG TTCAGATGTC
    CCTTCTAGAC AGATATCGAA CGTGACCCTC AGCGAGAGCT TGAAGCCAAA AGAGACCGCA
    GAATTTAGTT CTACGCAGTC TTTTCCCAAA TCTAGAGAAT CTCAATCTAG ACTCCATGGT
    ACTACAAGCA GCACCCTGTT GGGATCGAGC ACCCAGCAGC ACCCAGATAC CCCACAGCGT
    ACAGCCGAGA CCTCGCCAGA TGGTGGGGAG GTAAAACGCC AGAAGACCGC CTCCAGTACC
    ATCACCCTGA AGTCTGTCAA GATAGAAACC CAACCCAAGT CTGAGATAAG TCATGGTGGG
    ACCAGGCACA ACCAGCAGTC ACTTCCCTTG GGCCGGGAGG GGTCTCATTC ACACCCGGGG
    TTGTCGGACA CAAAAGAAAC CTTGAGCAGA AGCCTTGGAA CTGCCCCCGG CTTCGACCTG
    AGTCCCTCAA GCCAAGAGGT GCCGGAGGGC AGCGAAGTGA TATTCAGGTG CAAAGTTTCG
    GGAGATCCCA GACCGCGGGT CGAGTGGTTC CGAGAGAATT CGGCGCTGGG GGAGAGGGAC
    GGGGTGCGGA TCAGGGAAGG GGCTCGGATT CACTCGCTGC ACTTGCTGAA GGCACAGACG
    GGAGACAGCG GGAATTACAG CTGTGAAGTC TCCAACGCGA GAGGGCGGGT GTCTCATAGC
    TGGACCCTGA GCGTCACGCG GCGCAAGATG GAGGCGGTCG CGCCTCGGTT TGTCCGCAAG
    CTACGGGGCT GCTCCATTAT AGAGGGGGAG GATTTTGTGT TGGAATGTTC CATTGAGGGG
    CAACCGGCTC CTGAGATCTC GTGGCTGCTG AACGACCAGC CCATACAATA TGCCCACTCA
    GCGTATGAAG ACGGGGTGGC GAGGCTGCGG GTACAGGATG CCCTGCCGGA GGATGAGGGG
    GTATATACCT GCCTGGCACA GAACCGCGGC GGGAAGGTGT CCTGTTGTGC AACCGTCACT
    GTGACCGAAA AGAAGGCGGC AGAGGAGCCG GGGGGCGACC GAGAGCCAAA GAGCAGCCCC
    CCAATATTCC TGAAGCGCCT CACTGACCTG CAGGTTATGG ATGGGAGTCA AGTCACCATG
    AGCGTTGAAC TGTCAGGGAC CCCCCCTGCC GAGATCCTTT GGTTCCATAA TGGGAAAGAG
    ATTCAGGAGA CGGAGGATTT CCATTTTGAG CAGAGTGGTA ACGAGCACCG TCTGTGCATC
    CAGGAAGTCT TCCCCGAGGA CACAGGAACC TACATGTGCA AAGCCTGGAA TAGTTTGGGC
    CAAACTGCAA CGGAGGCCAC CTTAACCGTA CAAGAGCCGC AGGAGGGAAT CCAGCCATGG
    TTTATCAGCA AACCAAAGTG CACCAGCGCT TCAGTGGGGC AGAATGTGAT CCTATCCTGC
    GCCATCGCCG GGGACCCCTT CCCGCGGGTA CAATGGCTGA AGGACGGGCG CGAGTTGCCC
    CCCGAGCGGA TCCTGCAGAG CGACGACGTC TTCACTCTCA TCCTCAGAAA CGTCCAGCAG
    AGCGACGGCG GCCAATACCA GATCACGCTG ATCAATGCGG TGGGAGAATG CAGCTGCCGG
    GTCTCGCTGA TGGTCGAGGG AAGCTCCGGG GGTCCCTCTC ATACACAGAC TCTCAGGCAG
    AGGGAGCAGC TGCGCTCTTG CGAGAGGGAT GGATGGGATG AGGAGGATGC TCTGAGTCTT
    GGCAGGACTA CTGAGGAGCA ACAGGAGAAC ATTCGAGGGG TTCTCAAGAG GAGAGTTGAG
    GTCAAGGAGA GCTCGGAGGA GAAGCTCCGG CAGCAAGAGG CGGAACAAGT CGACTTCCGT
    AGGGTTCTGG GCAAAAAGGT GAGCACGAGG AGCGTTTCCG AGGAGGATTT GAAGGACATC
    CCACCGGAGC AGATGGACTT CCGAGCCAAC CTCCAGCGGC AGGTCAAACC TAAGGCTGAG
    GATGAACGCA AGGTCAATAG CCCACAGCAG GTGGACTTCC GCTCGGTACT GGGCAAGAAG
    CCATCGACTC CCAAAACCCC TCTTCCAGAG AAGGTGGTCG CCAAGGCAGC AACTCCTGAC
    TTCAGGTCGG TCCTCGGTGG CAAGAAAAAG CTTCCCACTG AGAATGGCAG CCCCAACGAC
    GTTCCACATG AACAGGGCAA ATCAAAGGTG AACTGCTGCA CGGTAGTGGA TGGGGGGGTT
    GCCAAGAACA GCGTGACCAA CAACAGTGTC CCTAATGCAG CCAAGATCAG TGTCCTCAGT
    GTGGACAAGG CCAATGTCCC AGGTACAGCC AAGAACAGTG TCCCCAGTGT GGACAAGACC
    AGTGCCCCAG GTACAGCCAA GAACAGTGTC CCCAGTGTGG ACAAGACCAG TGCCCCAAGT
    ACAGCCAAGA ACAGTGTCTC AAGTGGATCC AAGGCCAGTT TCCTAGGTGC GGATAAGGCC
    GGTGTCCCAA GTGTGGCCAA GACCAGTGTC CCAACTGCAA CCAAGACCAA TGTCCCAAGT
    GTGGACAAGA CTAATGTCCC TGCCGCAACC AAGACCAATG TCCCAAGTTT GGCCAAGACC
    AGTCTTCCAA CTGCAACCAA GACCAATGTC CCAAGTGTGG ACAAGGCCAG TGTCCCAAGT
    GTGGACAAGT CTAGTGTCCC TGCTGCAGTC AAGACCAATG ATAAGGACGT TGCCCCAAGT
    GCAGACAAGC AGCTAACCCC AAATGTGGAT AAAGATATTG CCCCAAGTTC AGAAAAGGAC
    GTTGCCCCAA GTGTGGACAA GGACGTTGCC CCAAGTTCAG AGAAGGACGT TGCCCCAAGT
    GTGGACAAGG ACATTGCCCC AAGTGTGGAC AAGGACATTG CCCCAAGTTC AGACAAAGAC
    GTTGCCCCCA GTTCAGACAA GGATGTTGCC CCCAGTTCAG ACAAGGATGT TGCCCCCGGT
    TCAGACAAGG ATGTTGCCCC CAGTTCAGAC AAGGATGTTG CCCCCAGTTC AGAGAAGGAC
    GTTGCCCCGA GTTCAGAGAA GGACGTTGCC CCAAATTCAG AGAAGGACGT TGCCCCAAAT
    TCAGAGAAGG ACGTTGCCCC AAGTTCAGAA AAGGACGTTG CCCCAAGTGC GCCCACAAAA
    CCCGAGTTTT CGGAGGTACT GCAGGATGTA CGTGTTACCG ATGGGGAGAA GCTGGTGTTG
    CAGTGCCGCG TCACAGCCGA ACCACCTCCG ACCGTTGTGT GGACTCTGGA TGGAAAAGTC
    ATCAAGTCCT CCAAGTACAT TGTCCTGTCC CAAGAAGGCT CCGTGTGTTC CCTGAGCATC
    GAGAAGACCT TCCCGGAGGA CGAGGGGCAG TACCTGTGCG CGGTGGAGAA TGCGGCCGGG
    AGGGCAGAAT GTGCCTGTAA TGTCACGGTG GAAGATACCT CGGGCAACAA GGGCATCAAG
    AAGAGCAAGA AGCCGAGAAG CAACGCTCCG CTCTCCAACG CAACGGAAAA TGAAGCTACG
    ATTAAGAAGA AGCCGGCTCC GAAGACCCCC CCCAAAGCAG CATCTGCCCC TCAGATCCTA
    CAGTTCCCAG AAGACAGGAA GGTGAAAGCC GGGGAGTCGG TCGAGCTCCT GTGCAAGGTG
    GGGGGGACCC AGCCCATTAC CTGCATGTGG ATGAAGTTCA GGAAGGAGAT TAAAGAAAGT
    GAATTTATCA AGATTGAGAG CACAGAAGAG ACCAGTAAGC TGACAATCTC CTCGGCCCGA
    CAGGAGCACT GCGGCTGCTA CACCCTGGTG GCGGAGAACA AACAGGGCAG CAAACAGGCG
    CAGGTTAACC TGACTGTGTT TGATAAACCC GACCCGCCGG CGGGGGTGCC CTGTGCCTCC
    TGTATGAAGG GCTCGGTGCT CACCCTGTCG TGGTACGGCC CCACGTATGA TGGGGGCAGT
    GTGGTCGAGT CCTACTCGGT GGAGATCTGG GACTCAGTGG GCAAAGTCTG GACGGATCTG
    ACCTCCTGCC GCAGCACCTC CTTTAATGTG GAGAATTTAC TGCAGGACAG AGAGTACAAA
    TTCCGGGTCC GGGCCACTAA CATCCACGGC ACCAGCGAAC CCAGTCAGGA GTCGGAACCA
    GTTACCTACA AGGAGAAGGA AGAAGAACAC AAAGAAGAAG AGGCAGAAGT TTCAGACGAC
    GAGGAGAAGG AGTCGGAGCC GGAATACAGG GACGTCACTC TCAACAGCAG CGAGAAAGTG
    ACCGACTACT ACGATGTGGA GGAGAGACTG GGCACGGGCA AATTTGGCCA AGTCTTCCGA
    ATCGTAGAGA AGAAGAGCAG GAAAATCTAT GCCGGGAAGT TCTTCAAAGC CTATTCGGCC
    AAAGACAAGG AGGGCATCCG GCAAGAAATT GCCATCATGA ACTGCCTGCA CCACCCCAAG
    CTGGTCCAGT GCGTCGACGC GTTTGAGTCC AAAGCCGACA TGGTGATGGT TCTGGAACTG
    GTCTCGGGCG GGGAACTCTT CGAGCGAATC ATTGACGAGG ATTTTGAGCT GACGGAGCGG
    GAGGTGATTA AGTACATGAA GCAGATCTGC GAGGGGGTCG AGTTCATCCA CAAACAGGGG
    ATCGTGCACC TCGACCTCAA ACCCGAGAAC ATCATGTGCG TCAATAAGAC TGGCACCAAG
    ATCAAACTCA TCGACTTCGG CCTCGCCCGC AGATTGGAGA GCTGCCCGAA CCTCAAAGTG
    CTTTTTGGAA CCCCTGAATT TGTAGCCCCC GAAGTGATAA ACTACGAGCC GATTGGTTAC
    TCGACGGACA TGTGGAGCAT CGGGGTCATC TGCTACATCT TGGTCAGTGG ACTTTCTCCA
    TTCATGGGGG ATAATGACAA TGAAACGCTG GCGAATGTAA CTTCGGCCAC TTGGGACTTC
    GATGATGAAG CCTTTGATGA AATATCTGAA GAAGCCAAAA ACTTAATCAG CAGTTTGTTG
    AAGAAGGACA TGAAGAATCG GCTGAACTGT ACCCAGTGCC TCCAGCATTC GTGGCTACAG
    CAGGACACTG CCACAATGGA AGCCAAGAAA CTCTCCAAAG ACAGGATGAA GAAATATATG
    GCTCGGAGGA AATGGCAGAA AACCGGGCAC GCAGTGAGGG CAATCGGACG TCTCTCCTCT
    ATGGCCATGA TCTCTGGGCT GAGCGCCAGG AAGTCATCGG GCAGCGCCCC CAGCAGCCCC
    CTGTCTGCGG AGCTTGAAGC CAATGAAGAA GACAACCAGG CTTTCCTGGA GTCGGTGACA
    GAGGAGAAGC CCCCCGCAAA GCCTTTCTTC ACGAAGACCA TTCTGGATAT CGAAGTCGTG
    GAAGGGAGCG CCGCGAGGTT TGACTGCAGA ATTGAGGGGC ACCCCGACCC GGAGGTGATT
    TGGTACAAGG ACGACCAACC CATCAAGGAG TCTCGGCACT TCCAGATCGA TTACGACGAG
    GACGGTAACT GCTCCCTGAC CATCTCGGAA GTTTGCGGCG ACGACGACGC CAAATACACC
    TGCAAAGCCG CCAACAGCCT GGGGGATACG ACCTGCACGG CCGAGCTGAT TGTAGAGGTG
    ATGGCGGAGG AGGAGGAGGA AGAGGAGGAG GAAGAGGAGG AGGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa67274
    Clone ID Related Accession (Same CDS sequence) XM_031893658.1
    Accession Version XM_031893658.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product myosin light chain kinase, smooth muscle isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGTTTTTAT CGAAGGATAA AACCAGCGCC AAAGAACCTC CTTATCTTGG GGTGTCGGTT 
    CTAGACTCCA AGAACAAGAG CGTTCCACCA AAAGGCCCCG GAGACATCAT CCAAAAAGAG
    CCACTTGGAC GGACCCCGCG GGAACCCACC CAGGAGTTTA TAAGAGATTC CCCATCTGAT
    GGTTTCACAA AAGAGACGTC TTCCAAAAAG TTGCCTTCTG TAATGAACAG AGAGACCTCG
    TTCTTGTCGG CACGGGAGCG GATAATGGGG CCCACTATAA AGTCGCCTTA CGGTTCTGCT
    AAGACTTTAG GGAACCCGGC TTCTCTGTAT GAAATGTCTA AAGAGACCCG TTCAGATGTC
    CCTTCTAGAC AGATATCGAA CGTGACCCTC AGCGAGAGCT TGAAGCCAAA AGAGACCGCA
    GAATTTAGTT CTACGCAGTC TTTTCCCAAA TCTAGAGAAT CTCAATCTAG ACTCCATGGT
    ACTACAAGCA GCACCCTGTT GGGATCGAGC ACCCAGCAGC ACCCAGATAC CCCACAGCGT
    ACAGCCGAGA CCTCGCCAGA TGGTGGGGAG GTAAAACGCC AGAAGACCGC CTCCAGTACC
    ATCACCCTGA AGTCTGTCAA GATAGAAACC CAACCCAAGT CTGAGATAAG TCATGGTGGG
    ACCAGGCACA ACCAGCAGTC ACTTCCCTTG GGCCGGGAGG GGTCTCATTC ACACCCGGGG
    TTGTCGGACA CAAAAGAAAC CTTGAGCAGA AGCCTTGGAA CTGCCCCCGG CTTCGACCTG
    AGTCCCTCAA GCCAAGAGGT GCCGGAGGGC AGCGAAGTGA TATTCAGGTG CAAAGTTTCG
    GGAGATCCCA GACCGCGGGT CGAGTGGTTC CGAGAGAATT CGGCGCTGGG GGAGAGGGAC
    GGGGTGCGGA TCAGGGAAGG GGCTCGGATT CACTCGCTGC ACTTGCTGAA GGCACAGACG
    GGAGACAGCG GGAATTACAG CTGTGAAGTC TCCAACGCGA GAGGGCGGGT GTCTCATAGC
    TGGACCCTGA GCGTCACGCG GCGCAAGATG GAGGCGGTCG CGCCTCGGTT TGTCCGCAAG
    CTACGGGGCT GCTCCATTAT AGAGGGGGAG GATTTTGTGT TGGAATGTTC CATTGAGGGG
    CAACCGGCTC CTGAGATCTC GTGGCTGCTG AACGACCAGC CCATACAATA TGCCCACTCA
    GCGTATGAAG ACGGGGTGGC GAGGCTGCGG GTACAGGATG CCCTGCCGGA GGATGAGGGG
    GTATATACCT GCCTGGCACA GAACCGCGGC GGGAAGGTGT CCTGTTGTGC AACCGTCACT
    GTGACCGAAA AGAAGGCGGC AGAGGAGCCG GGGGGCGACC GAGAGCCAAA GAGCAGCCCC
    CCAATATTCC TGAAGCGCCT CACTGACCTG CAGGTTATGG ATGGGAGTCA AGTCACCATG
    AGCGTTGAAC TGTCAGGGAC CCCCCCTGCC GAGATCCTTT GGTTCCATAA TGGGAAAGAG
    ATTCAGGAGA CGGAGGATTT CCATTTTGAG CAGAGTGGTA ACGAGCACCG TCTGTGCATC
    CAGGAAGTCT TCCCCGAGGA CACAGGAACC TACATGTGCA AAGCCTGGAA TAGTTTGGGC
    CAAACTGCAA CGGAGGCCAC CTTAACCGTA CAAGAGCCGC AGGAGGGAAT CCAGCCATGG
    TTTATCAGCA AACCAAAGTG CACCAGCGCT TCAGTGGGGC AGAATGTGAT CCTATCCTGC
    GCCATCGCCG GGGACCCCTT CCCGCGGGTA CAATGGCTGA AGGACGGGCG CGAGTTGCCC
    CCCGAGCGGA TCCTGCAGAG CGACGACGTC TTCACTCTCA TCCTCAGAAA CGTCCAGCAG
    AGCGACGGCG GCCAATACCA GATCACGCTG ATCAATGCGG TGGGAGAATG CAGCTGCCGG
    GTCTCGCTGA TGGTCGAGGG AAGCTCCGGG GGTCCCTCTC ATACACAGCA GAGGGAGCAG
    CTGCGCTCTT GCGAGAGGGA TGGATGGGAT GAGGAGGATG CTCTGAGTCT TGGCAGGACT
    ACTGAGGAGC AACAGGAGAA CATTCGAGGG GTTCTCAAGA GGAGAGTTGA GGTCAAGGAG
    AGCTCGGAGG AGAAGCTCCG GCAGCAAGAG GCGGAACAAG TCGACTTCCG TAGGGTTCTG
    GGCAAAAAGG TGAGCACGAG GAGCGTTTCC GAGGAGGATT TGAAGGACAT CCCACCGGAG
    CAGATGGACT TCCGAGCCAA CCTCCAGCGG CAGGTCAAAC CTAAGGCTGA GGATGAACGC
    AAGGTCAATA GCCCACAGCA GGTGGACTTC CGCTCGGTAC TGGGCAAGAA GCCATCGACT
    CCCAAAACCC CTCTTCCAGA GAAGGTGGTC GCCAAGGCAG CAACTCCTGA CTTCAGGTCG
    GTCCTCGGTG GCAAGAAAAA GCTTCCCACT GAGAATGGCA GCCCCAACGA CGTTCCACAT
    GAACAGGGCA AATCAAAGGT GAACTGCTGC ACGGTAGTGG ATGGGGGGGT TGCCAAGAAC
    AGCGTGACCA ACAACAGTGT CCCTAATGCA GCCAAGATCA GTGTCCTCAG TGTGGACAAG
    GCCAATGTCC CAGGTACAGC CAAGAACAGT GTCCCCAGTG TGGACAAGAC CAGTGCCCCA
    GGTACAGCCA AGAACAGTGT CCCCAGTGTG GACAAGACCA GTGCCCCAAG TACAGCCAAG
    AACAGTGTCT CAAGTGGATC CAAGGCCAGT TTCCTAGGTG CGGATAAGGC CGGTGTCCCA
    AGTGTGGCCA AGACCAGTGT CCCAACTGCA ACCAAGACCA ATGTCCCAAG TGTGGACAAG
    ACTAATGTCC CTGCCGCAAC CAAGACCAAT GTCCCAAGTT TGGCCAAGAC CAGTCTTCCA
    ACTGCAACCA AGACCAATGT CCCAAGTGTG GACAAGGCCA GTGTCCCAAG TGTGGACAAG
    TCTAGTGTCC CTGCTGCAGT CAAGACCAAT GATAAGGACG TTGCCCCAAG TGCAGACAAG
    CAGCTAACCC CAAATGTGGA TAAAGATATT GCCCCAAGTT CAGAAAAGGA CGTTGCCCCA
    AGTGTGGACA AGGACGTTGC CCCAAGTTCA GAGAAGGACG TTGCCCCAAG TGTGGACAAG
    GACATTGCCC CAAGTGTGGA CAAGGACATT GCCCCAAGTT CAGACAAAGA CGTTGCCCCC
    AGTTCAGACA AGGATGTTGC CCCCAGTTCA GACAAGGATG TTGCCCCCGG TTCAGACAAG
    GATGTTGCCC CCAGTTCAGA CAAGGATGTT GCCCCCAGTT CAGAGAAGGA CGTTGCCCCG
    AGTTCAGAGA AGGACGTTGC CCCAAATTCA GAGAAGGACG TTGCCCCAAA TTCAGAGAAG
    GACGTTGCCC CAAGTTCAGA AAAGGACGTT GCCCCAAGTG CGCCCACAAA ACCCGAGTTT
    TCGGAGGTAC TGCAGGATGT ACGTGTTACC GATGGGGAGA AGCTGGTGTT GCAGTGCCGC
    GTCACAGCCG AACCACCTCC GACCGTTGTG TGGACTCTGG ATGGAAAAGT CATCAAGTCC
    TCCAAGTACA TTGTCCTGTC CCAAGAAGGC TCCGTGTGTT CCCTGAGCAT CGAGAAGACC
    TTCCCGGAGG ACGAGGGGCA GTACCTGTGC GCGGTGGAGA ATGCGGCCGG GAGGGCAGAA
    TGTGCCTGTA ATGTCACGGT GGAAGATACC TCGGGCAACA AGGGCATCAA GAAGAGCAAG
    AAGCCGAGAA GCAACGCTCC GCTCTCCAAC GCAACGGAAA ATGAAGCTAC GATTAAGAAG
    AAGCCGGCTC CGAAGACCCC CCCCAAAGCA GCATCTGCCC CTCAGATCCT ACAGTTCCCA
    GAAGACAGGA AGGTGAAAGC CGGGGAGTCG GTCGAGCTCC TGTGCAAGGT GGGGGGGACC
    CAGCCCATTA CCTGCATGTG GATGAAGTTC AGGAAGGAGA TTAAAGAAAG TGAATTTATC
    AAGATTGAGA GCACAGAAGA GACCAGTAAG CTGACAATCT CCTCGGCCCG ACAGGAGCAC
    TGCGGCTGCT ACACCCTGGT GGCGGAGAAC AAACAGGGCA GCAAACAGGC GCAGGTTAAC
    CTGACTGTGT TTGATAAACC CGACCCGCCG GCGGGGGTGC CCTGTGCCTC CTGTATGAAG
    GGCTCGGTGC TCACCCTGTC GTGGTACGGC CCCACGTATG ATGGGGGCAG TGTGGTCGAG
    TCCTACTCGG TGGAGATCTG GGACTCAGTG GGCAAAGTCT GGACGGATCT GACCTCCTGC
    CGCAGCACCT CCTTTAATGT GGAGAATTTA CTGCAGGACA GAGAGTACAA ATTCCGGGTC
    CGGGCCACTA ACATCCACGG CACCAGCGAA CCCAGTCAGG AGTCGGAACC AGTTACCTAC
    AAGGAGAAGG AAGAAGAACA CAAAGAAGAA GAGGCAGAAG TTTCAGACGA CGAGGAGAAG
    GAGTCGGAGC CGGAATACAG GGACGTCACT CTCAACAGCA GCGAGAAAGT GACCGACTAC
    TACGATGTGG AGGAGAGACT GGGCACGGGC AAATTTGGCC AAGTCTTCCG AATCGTAGAG
    AAGAAGAGCA GGAAAATCTA TGCCGGGAAG TTCTTCAAAG CCTATTCGGC CAAAGACAAG
    GAGGGCATCC GGCAAGAAAT TGCCATCATG AACTGCCTGC ACCACCCCAA GCTGGTCCAG
    TGCGTCGACG CGTTTGAGTC CAAAGCCGAC ATGGTGATGG TTCTGGAACT GGTCTCGGGC
    GGGGAACTCT TCGAGCGAAT CATTGACGAG GATTTTGAGC TGACGGAGCG GGAGGTGATT
    AAGTACATGA AGCAGATCTG CGAGGGGGTC GAGTTCATCC ACAAACAGGG GATCGTGCAC
    CTCGACCTCA AACCCGAGAA CATCATGTGC GTCAATAAGA CTGGCACCAA GATCAAACTC
    ATCGACTTCG GCCTCGCCCG CAGATTGGAG AGCTGCCCGA ACCTCAAAGT GCTTTTTGGA
    ACCCCTGAAT TTGTAGCCCC CGAAGTGATA AACTACGAGC CGATTGGTTA CTCGACGGAC
    ATGTGGAGCA TCGGGGTCAT CTGCTACATC TTGGTCAGTG GACTTTCTCC ATTCATGGGG
    GATAATGACA ATGAAACGCT GGCGAATGTA ACTTCGGCCA CTTGGGACTT CGATGATGAA
    GCCTTTGATG AAATATCTGA AGAAGCCAAA AACTTAATCA GCAGTTTGTT GAAGAAGGAC
    ATGAAGAATC GGCTGAACTG TACCCAGTGC CTCCAGCATT CGTGGCTACA GCAGGACACT
    GCCACAATGG AAGCCAAGAA ACTCTCCAAA GACAGGATGA AGAAATATAT GGCTCGGAGG
    AAATGGCAGA AAACCGGGCA CGCAGTGAGG GCAATCGGAC GTCTCTCCTC TATGGCCATG
    ATCTCTGGGC TGAGCGCCAG GAAGTCATCG GGCAGCGCCC CCAGCAGCCC CCTGTCTGCG
    GAGCTTGAAG CCAATGAAGA AGACAACCAG GCTTTCCTGG AGTCGGTGAC AGAGGAGAAG
    CCCCCCGCAA AGCCTTTCTT CACGAAGACC ATTCTGGATA TCGAAGTCGT GGAAGGGAGC
    GCCGCGAGGT TTGACTGCAG AATTGAGGGG CACCCCGACC CGGAGGTGAT TTGGTACAAG
    GACGACCAAC CCATCAAGGA GTCTCGGCAC TTCCAGATCG ATTACGACGA GGACGGTAAC
    TGCTCCCTGA CCATCTCGGA AGTTTGCGGC GACGACGACG CCAAATACAC CTGCAAAGCC
    GCCAACAGCC TGGGGGATAC GACCTGCACG GCCGAGCTGA TTGTAGAGGT GATGGCGGAG
    GAGGAGGAGG AAGAGGAGGA GGAAGAGGAG GAGGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031749518.1
    CDS516..6374
    Translation

    Target ORF information:

    RefSeq Version XM_031893658.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis myosin light chain kinase (mylk), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031893658.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGTTTTTAT CGAAGGATAA AACCAGCGCC AAAGAACCTC CTTATCTTGG GGTGTCGGTT 
    CTAGACTCCA AGAACAAGAG CGTTCCACCA AAAGGCCCCG GAGACATCAT CCAAAAAGAG
    CCACTTGGAC GGACCCCGCG GGAACCCACC CAGGAGTTTA TAAGAGATTC CCCATCTGAT
    GGTTTCACAA AAGAGACGTC TTCCAAAAAG TTGCCTTCTG TAATGAACAG AGAGACCTCG
    TTCTTGTCGG CACGGGAGCG GATAATGGGG CCCACTATAA AGTCGCCTTA CGGTTCTGCT
    AAGACTTTAG GGAACCCGGC TTCTCTGTAT GAAATGTCTA AAGAGACCCG TTCAGATGTC
    CCTTCTAGAC AGATATCGAA CGTGACCCTC AGCGAGAGCT TGAAGCCAAA AGAGACCGCA
    GAATTTAGTT CTACGCAGTC TTTTCCCAAA TCTAGAGAAT CTCAATCTAG ACTCCATGGT
    ACTACAAGCA GCACCCTGTT GGGATCGAGC ACCCAGCAGC ACCCAGATAC CCCACAGCGT
    ACAGCCGAGA CCTCGCCAGA TGGTGGGGAG GTAAAACGCC AGAAGACCGC CTCCAGTACC
    ATCACCCTGA AGTCTGTCAA GATAGAAACC CAACCCAAGT CTGAGATAAG TCATGGTGGG
    ACCAGGCACA ACCAGCAGTC ACTTCCCTTG GGCCGGGAGG GGTCTCATTC ACACCCGGGG
    TTGTCGGACA CAAAAGAAAC CTTGAGCAGA AGCCTTGGAA CTGCCCCCGG CTTCGACCTG
    AGTCCCTCAA GCCAAGAGGT GCCGGAGGGC AGCGAAGTGA TATTCAGGTG CAAAGTTTCG
    GGAGATCCCA GACCGCGGGT CGAGTGGTTC CGAGAGAATT CGGCGCTGGG GGAGAGGGAC
    GGGGTGCGGA TCAGGGAAGG GGCTCGGATT CACTCGCTGC ACTTGCTGAA GGCACAGACG
    GGAGACAGCG GGAATTACAG CTGTGAAGTC TCCAACGCGA GAGGGCGGGT GTCTCATAGC
    TGGACCCTGA GCGTCACGCG GCGCAAGATG GAGGCGGTCG CGCCTCGGTT TGTCCGCAAG
    CTACGGGGCT GCTCCATTAT AGAGGGGGAG GATTTTGTGT TGGAATGTTC CATTGAGGGG
    CAACCGGCTC CTGAGATCTC GTGGCTGCTG AACGACCAGC CCATACAATA TGCCCACTCA
    GCGTATGAAG ACGGGGTGGC GAGGCTGCGG GTACAGGATG CCCTGCCGGA GGATGAGGGG
    GTATATACCT GCCTGGCACA GAACCGCGGC GGGAAGGTGT CCTGTTGTGC AACCGTCACT
    GTGACCGAAA AGAAGGCGGC AGAGGAGCCG GGGGGCGACC GAGAGCCAAA GAGCAGCCCC
    CCAATATTCC TGAAGCGCCT CACTGACCTG CAGGTTATGG ATGGGAGTCA AGTCACCATG
    AGCGTTGAAC TGTCAGGGAC CCCCCCTGCC GAGATCCTTT GGTTCCATAA TGGGAAAGAG
    ATTCAGGAGA CGGAGGATTT CCATTTTGAG CAGAGTGGTA ACGAGCACCG TCTGTGCATC
    CAGGAAGTCT TCCCCGAGGA CACAGGAACC TACATGTGCA AAGCCTGGAA TAGTTTGGGC
    CAAACTGCAA CGGAGGCCAC CTTAACCGTA CAAGAGCCGC AGGAGGGAAT CCAGCCATGG
    TTTATCAGCA AACCAAAGTG CACCAGCGCT TCAGTGGGGC AGAATGTGAT CCTATCCTGC
    GCCATCGCCG GGGACCCCTT CCCGCGGGTA CAATGGCTGA AGGACGGGCG CGAGTTGCCC
    CCCGAGCGGA TCCTGCAGAG CGACGACGTC TTCACTCTCA TCCTCAGAAA CGTCCAGCAG
    AGCGACGGCG GCCAATACCA GATCACGCTG ATCAATGCGG TGGGAGAATG CAGCTGCCGG
    GTCTCGCTGA TGGTCGAGGG AAGCTCCGGG GGTCCCTCTC ATACACAGCA GAGGGAGCAG
    CTGCGCTCTT GCGAGAGGGA TGGATGGGAT GAGGAGGATG CTCTGAGTCT TGGCAGGACT
    ACTGAGGAGC AACAGGAGAA CATTCGAGGG GTTCTCAAGA GGAGAGTTGA GGTCAAGGAG
    AGCTCGGAGG AGAAGCTCCG GCAGCAAGAG GCGGAACAAG TCGACTTCCG TAGGGTTCTG
    GGCAAAAAGG TGAGCACGAG GAGCGTTTCC GAGGAGGATT TGAAGGACAT CCCACCGGAG
    CAGATGGACT TCCGAGCCAA CCTCCAGCGG CAGGTCAAAC CTAAGGCTGA GGATGAACGC
    AAGGTCAATA GCCCACAGCA GGTGGACTTC CGCTCGGTAC TGGGCAAGAA GCCATCGACT
    CCCAAAACCC CTCTTCCAGA GAAGGTGGTC GCCAAGGCAG CAACTCCTGA CTTCAGGTCG
    GTCCTCGGTG GCAAGAAAAA GCTTCCCACT GAGAATGGCA GCCCCAACGA CGTTCCACAT
    GAACAGGGCA AATCAAAGGT GAACTGCTGC ACGGTAGTGG ATGGGGGGGT TGCCAAGAAC
    AGCGTGACCA ACAACAGTGT CCCTAATGCA GCCAAGATCA GTGTCCTCAG TGTGGACAAG
    GCCAATGTCC CAGGTACAGC CAAGAACAGT GTCCCCAGTG TGGACAAGAC CAGTGCCCCA
    GGTACAGCCA AGAACAGTGT CCCCAGTGTG GACAAGACCA GTGCCCCAAG TACAGCCAAG
    AACAGTGTCT CAAGTGGATC CAAGGCCAGT TTCCTAGGTG CGGATAAGGC CGGTGTCCCA
    AGTGTGGCCA AGACCAGTGT CCCAACTGCA ACCAAGACCA ATGTCCCAAG TGTGGACAAG
    ACTAATGTCC CTGCCGCAAC CAAGACCAAT GTCCCAAGTT TGGCCAAGAC CAGTCTTCCA
    ACTGCAACCA AGACCAATGT CCCAAGTGTG GACAAGGCCA GTGTCCCAAG TGTGGACAAG
    TCTAGTGTCC CTGCTGCAGT CAAGACCAAT GATAAGGACG TTGCCCCAAG TGCAGACAAG
    CAGCTAACCC CAAATGTGGA TAAAGATATT GCCCCAAGTT CAGAAAAGGA CGTTGCCCCA
    AGTGTGGACA AGGACGTTGC CCCAAGTTCA GAGAAGGACG TTGCCCCAAG TGTGGACAAG
    GACATTGCCC CAAGTGTGGA CAAGGACATT GCCCCAAGTT CAGACAAAGA CGTTGCCCCC
    AGTTCAGACA AGGATGTTGC CCCCAGTTCA GACAAGGATG TTGCCCCCGG TTCAGACAAG
    GATGTTGCCC CCAGTTCAGA CAAGGATGTT GCCCCCAGTT CAGAGAAGGA CGTTGCCCCG
    AGTTCAGAGA AGGACGTTGC CCCAAATTCA GAGAAGGACG TTGCCCCAAA TTCAGAGAAG
    GACGTTGCCC CAAGTTCAGA AAAGGACGTT GCCCCAAGTG CGCCCACAAA ACCCGAGTTT
    TCGGAGGTAC TGCAGGATGT ACGTGTTACC GATGGGGAGA AGCTGGTGTT GCAGTGCCGC
    GTCACAGCCG AACCACCTCC GACCGTTGTG TGGACTCTGG ATGGAAAAGT CATCAAGTCC
    TCCAAGTACA TTGTCCTGTC CCAAGAAGGC TCCGTGTGTT CCCTGAGCAT CGAGAAGACC
    TTCCCGGAGG ACGAGGGGCA GTACCTGTGC GCGGTGGAGA ATGCGGCCGG GAGGGCAGAA
    TGTGCCTGTA ATGTCACGGT GGAAGATACC TCGGGCAACA AGGGCATCAA GAAGAGCAAG
    AAGCCGAGAA GCAACGCTCC GCTCTCCAAC GCAACGGAAA ATGAAGCTAC GATTAAGAAG
    AAGCCGGCTC CGAAGACCCC CCCCAAAGCA GCATCTGCCC CTCAGATCCT ACAGTTCCCA
    GAAGACAGGA AGGTGAAAGC CGGGGAGTCG GTCGAGCTCC TGTGCAAGGT GGGGGGGACC
    CAGCCCATTA CCTGCATGTG GATGAAGTTC AGGAAGGAGA TTAAAGAAAG TGAATTTATC
    AAGATTGAGA GCACAGAAGA GACCAGTAAG CTGACAATCT CCTCGGCCCG ACAGGAGCAC
    TGCGGCTGCT ACACCCTGGT GGCGGAGAAC AAACAGGGCA GCAAACAGGC GCAGGTTAAC
    CTGACTGTGT TTGATAAACC CGACCCGCCG GCGGGGGTGC CCTGTGCCTC CTGTATGAAG
    GGCTCGGTGC TCACCCTGTC GTGGTACGGC CCCACGTATG ATGGGGGCAG TGTGGTCGAG
    TCCTACTCGG TGGAGATCTG GGACTCAGTG GGCAAAGTCT GGACGGATCT GACCTCCTGC
    CGCAGCACCT CCTTTAATGT GGAGAATTTA CTGCAGGACA GAGAGTACAA ATTCCGGGTC
    CGGGCCACTA ACATCCACGG CACCAGCGAA CCCAGTCAGG AGTCGGAACC AGTTACCTAC
    AAGGAGAAGG AAGAAGAACA CAAAGAAGAA GAGGCAGAAG TTTCAGACGA CGAGGAGAAG
    GAGTCGGAGC CGGAATACAG GGACGTCACT CTCAACAGCA GCGAGAAAGT GACCGACTAC
    TACGATGTGG AGGAGAGACT GGGCACGGGC AAATTTGGCC AAGTCTTCCG AATCGTAGAG
    AAGAAGAGCA GGAAAATCTA TGCCGGGAAG TTCTTCAAAG CCTATTCGGC CAAAGACAAG
    GAGGGCATCC GGCAAGAAAT TGCCATCATG AACTGCCTGC ACCACCCCAA GCTGGTCCAG
    TGCGTCGACG CGTTTGAGTC CAAAGCCGAC ATGGTGATGG TTCTGGAACT GGTCTCGGGC
    GGGGAACTCT TCGAGCGAAT CATTGACGAG GATTTTGAGC TGACGGAGCG GGAGGTGATT
    AAGTACATGA AGCAGATCTG CGAGGGGGTC GAGTTCATCC ACAAACAGGG GATCGTGCAC
    CTCGACCTCA AACCCGAGAA CATCATGTGC GTCAATAAGA CTGGCACCAA GATCAAACTC
    ATCGACTTCG GCCTCGCCCG CAGATTGGAG AGCTGCCCGA ACCTCAAAGT GCTTTTTGGA
    ACCCCTGAAT TTGTAGCCCC CGAAGTGATA AACTACGAGC CGATTGGTTA CTCGACGGAC
    ATGTGGAGCA TCGGGGTCAT CTGCTACATC TTGGTCAGTG GACTTTCTCC ATTCATGGGG
    GATAATGACA ATGAAACGCT GGCGAATGTA ACTTCGGCCA CTTGGGACTT CGATGATGAA
    GCCTTTGATG AAATATCTGA AGAAGCCAAA AACTTAATCA GCAGTTTGTT GAAGAAGGAC
    ATGAAGAATC GGCTGAACTG TACCCAGTGC CTCCAGCATT CGTGGCTACA GCAGGACACT
    GCCACAATGG AAGCCAAGAA ACTCTCCAAA GACAGGATGA AGAAATATAT GGCTCGGAGG
    AAATGGCAGA AAACCGGGCA CGCAGTGAGG GCAATCGGAC GTCTCTCCTC TATGGCCATG
    ATCTCTGGGC TGAGCGCCAG GAAGTCATCG GGCAGCGCCC CCAGCAGCCC CCTGTCTGCG
    GAGCTTGAAG CCAATGAAGA AGACAACCAG GCTTTCCTGG AGTCGGTGAC AGAGGAGAAG
    CCCCCCGCAA AGCCTTTCTT CACGAAGACC ATTCTGGATA TCGAAGTCGT GGAAGGGAGC
    GCCGCGAGGT TTGACTGCAG AATTGAGGGG CACCCCGACC CGGAGGTGAT TTGGTACAAG
    GACGACCAAC CCATCAAGGA GTCTCGGCAC TTCCAGATCG ATTACGACGA GGACGGTAAC
    TGCTCCCTGA CCATCTCGGA AGTTTGCGGC GACGACGACG CCAAATACAC CTGCAAAGCC
    GCCAACAGCC TGGGGGATAC GACCTGCACG GCCGAGCTGA TTGTAGAGGT GATGGCGGAG
    GAGGAGGAGG AAGAGGAGGA GGAAGAGGAG GAGGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa67275
    Clone ID Related Accession (Same CDS sequence) XM_031893659.1
    Accession Version XM_031893659.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product myosin light chain kinase, smooth muscle isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGTTTTTAT CGAAGGATAA AACCAGCGCC AAAGAACCTC CTTATCTTGG GGTGTCGGTT 
    CTAGACTCCA AGAACAAGAG CGTTCCACCA AAAGGCCCCG GAGACATCAT CCAAAAAGAG
    CCACTTGGAC GGACCCCGCG GGAACCCACC CAGGAGTTTA TAAGAGATTC CCCATCTGAT
    GGTTTCACAA AAGAGACGTC TTCCAAAAAG TTGCCTTCTG TAATGAACAG AGAGACCTCG
    TTCTTGTCGG CACGGGAGCG GATAATGGGG CCCACTATAA AGTCGCCTTA CGGTTCTGCT
    AAGACTTTAG GGAACCCGGC TTCTCTGTAT GAAATGTCTA AAGAGACCCG TTCAGATGTC
    CCTTCTAGAC AGATATCGAA CGTGACCCTC AGCGAGAGCT TGAAGCCAAA AGAGACCGCA
    GAATTTAGTT CTACGCAGTC TTTTCCCAAA TCTAGAGAAT CTCAATCTAG ACTCCATGGT
    ACTACAAGCA GCACCCTGTT GGGATCGAGC ACCCAGCAGC ACCCAGATAC CCCACAGCGT
    ACAGCCGAGA CCTCGCCAGA TGGTGGGGAG GTAAAACGCC AGAAGACCGC CTCCAGTACC
    ATCACCCTGA AGTCTGTCAA GATAGAAACC CAACCCAAGT CTGAGATAAG TCATGGTGGG
    ACCAGGCACA ACCAGCAGTC ACTTCCCTTG GGCCGGGAGG GGTCTCATTC ACACCCGGGG
    TTGTCGGACA CAAAAGAAAC CTTGAGCAGA AGCCTTGGAA CTGCCCCCGG CTTCGACCTG
    AGTCCCTCAA GCCAAGAGGT GCCGGAGGGC AGCGAAGTGA TATTCAGGTG CAAAGTTTCG
    GGAGATCCCA GACCGCGGGT CGAGTGGTTC CGAGAGAATT CGGCGCTGGG GGAGAGGGAC
    GGGGTGCGGA TCAGGGAAGG GGCTCGGATT CACTCGCTGC ACTTGCTGAA GGCACAGACG
    GGAGACAGCG GGAATTACAG CTGTGAAGTC TCCAACGCGA GAGGGCGGGT GTCTCATAGC
    TGGACCCTGA GCGTCACGCG GCGCAAGATG GAGGCGGTCG CGCCTCGGTT TGTCCGCAAG
    CTACGGGGCT GCTCCATTAT AGAGGGGGAG GATTTTGTGT TGGAATGTTC CATTGAGGGG
    CAACCGGCTC CTGAGATCTC GTGGCTGCTG AACGACCAGC CCATACAATA TGCCCACTCA
    GCGTATGAAG ACGGGGTGGC GAGGCTGCGG GTACAGGATG CCCTGCCGGA GGATGAGGGG
    GTATATACCT GCCTGGCACA GAACCGCGGC GGGAAGGTGT CCTGTTGTGC AACCGTCACT
    GTGACCGAAA AGAAGGCGGC AGAGGAGCCG GGGGGCGACC GAGAGCCAAA GAGCAGCCCC
    CCAATATTCC TGAAGCGCCT CACTGACCTG CAGGTTATGG ATGGGAGTCA AGTCACCATG
    AGCGTTGAAC TGTCAGGGAC CCCCCCTGCC GAGATCCTTT GGTTCCATAA TGGGAAAGAG
    ATTCAGGAGA CGGAGGATTT CCATTTTGAG CAGAGTGGTA ACGAGCACCG TCTGTGCATC
    CAGGAAGTCT TCCCCGAGGA CACAGGAACC TACATGTGCA AAGCCTGGAA TAGTTTGGGC
    CAAACTGCAA CGGAGGCCAC CTTAACCGTA CAAGAGCCGC AGGAGGGAAT CCAGCCATGG
    TTTATCAGCA AACCAAAGTG CACCAGCGCT TCAGTGGGGC AGAATGTGAT CCTATCCTGC
    GCCATCGCCG GGGACCCCTT CCCGCGGGTA CAATGGCTGA AGGACGGGCG CGAGTTGCCC
    CCCGAGCGGA TCCTGCAGAG CGACGACGTC TTCACTCTCA TCCTCAGAAA CGTCCAGCAG
    AGCGACGGCG GCCAATACCA GATCACGCTG ATCAATGCGG TGGGAGAATG CAGCTGCCGG
    GTCTCGCTGA TGGTCGAGGG AAGCTCCGGG GGTCCCTCTC ATACACACCA AACCTCCCTG
    ATTGTCTCTT TCTGTCTCTT TCCCAATCGG ACTCTCAGGC AGAGGGAGCA GCTGCGCTCT
    TGCGAGAGGG ATGGATGGGA TGAGGAGGAT GCTCTGAGTC TTGGCAGGAC TACTGAGGAG
    CAACAGGAGA ACATTCGAGG GGTTCTCAAG AGGAGAGTTG AGGTCAAGGA GAGCTCGGAG
    GAGAAGCTCC GGCAGCAAGA GGCGGAACAA GTCGACTTCC GTAGGGTTCT GGGCAAAAAG
    GTGAGCACGA GGAGCGTTTC CGAGGAGGAT TTGAAGGACA TCCCACCGGA GCAGATGGAC
    TTCCGAGCCA ACCTCCAGCG GCAGGTCAAA CCTAAGGCTG AGGATGAACG CAAGGTCAAT
    AGCCCACAGC AGGTGGACTT CCGCTCGGTA CTGGGCAAGA AGCCATCGAC TCCCAAAACC
    CCTCTTCCAG AGAAGGTGGT CGCCAAGGCA GCAACTCCTG ACTTCAGGTC GGTCCTCGGT
    GGCAAGAAAA AGCTTCCCAC TGAGAATGGC AGCCCCAACG ACGTTCCACA TGAACAGGGC
    AAATCAAAGG TGAACTGCTG CACGGTAGTG GATGGGGGGG TTGCCAAGAA CAGCGTGACC
    AACAACAGTG TCCCTAATGC AGCCAAGATC AGTGTCCTCA GTGTGGACAA GGCCAATGTC
    CCAGGTACAG CCAAGAACAG TGTCCCCAGT GTGGACAAGA CCAGTGCCCC AGGTACAGCC
    AAGAACAGTG TCCCCAGTGT GGACAAGACC AGTGCCCCAA GTACAGCCAA GAACAGTGTC
    TCAAGTGGAT CCAAGGCCAG TTTCCTAGGT GCGGATAAGG CCGGTGTCCC AAGTGTGGCC
    AAGACCAGTG TCCCAACTGC AACCAAGACC AATGTCCCAA GTGTGGACAA GACTAATGTC
    CCTGCCGCAA CCAAGACCAA TGTCCCAAGT TTGGCCAAGA CCAGTCTTCC AACTGCAACC
    AAGACCAATG TCCCAAGTGT GGACAAGGCC AGTGTCCCAA GTGTGGACAA GTCTAGTGTC
    CCTGCTGCAG TCAAGACCAA TGATAAGGAC GTTGCCCCAA GTGCAGACAA GCAGCTAACC
    CCAAATGTGG ATAAAGATAT TGCCCCAAGT TCAGAAAAGG ACGTTGCCCC AAGTGTGGAC
    AAGGACGTTG CCCCAAGTTC AGAGAAGGAC GTTGCCCCAA GTGTGGACAA GGACATTGCC
    CCAAGTGTGG ACAAGGACAT TGCCCCAAGT TCAGACAAAG ACGTTGCCCC CAGTTCAGAC
    AAGGATGTTG CCCCCAGTTC AGACAAGGAT GTTGCCCCCG GTTCAGACAA GGATGTTGCC
    CCCAGTTCAG ACAAGGATGT TGCCCCCAGT TCAGAGAAGG ACGTTGCCCC GAGTTCAGAG
    AAGGACGTTG CCCCAAATTC AGAGAAGGAC GTTGCCCCAA ATTCAGAGAA GGACGTTGCC
    CCAAGTTCAG AAAAGGACGT TGCCCCAAGT GCGCCCACAA AACCCGAGTT TTCGGAGGTA
    CTGCAGGATG TACGTGTTAC CGATGGGGAG AAGCTGGTGT TGCAGTGCCG CGTCACAGCC
    GAACCACCTC CGACCGTTGT GTGGACTCTG GATGGAAAAG TCATCAAGTC CTCCAAGTAC
    ATTGTCCTGT CCCAAGAAGG CTCCGTGTGT TCCCTGAGCA TCGAGAAGAC CTTCCCGGAG
    GACGAGGGGC AGTACCTGTG CGCGGTGGAG AATGCGGCCG GGAGGGCAGA ATGTGCCTGT
    AATGTCACGG TGGAAGATAC CTCGGGCAAC AAGGGCATCA AGAAGAGCAA GAAGCCGAGA
    AGCAACGCTC CGCTCTCCAA CGCAACGGAA ACATCTGCCC CTCAGATCCT ACAGTTCCCA
    GAAGACAGGA AGGTGAAAGC CGGGGAGTCG GTCGAGCTCC TGTGCAAGGT GGGGGGGACC
    CAGCCCATTA CCTGCATGTG GATGAAGTTC AGGAAGGAGA TTAAAGAAAG TGAATTTATC
    AAGATTGAGA GCACAGAAGA GACCAGTAAG CTGACAATCT CCTCGGCCCG ACAGGAGCAC
    TGCGGCTGCT ACACCCTGGT GGCGGAGAAC AAACAGGGCA GCAAACAGGC GCAGGTTAAC
    CTGACTGTGT TTGATAAACC CGACCCGCCG GCGGGGGTGC CCTGTGCCTC CTGTATGAAG
    GGCTCGGTGC TCACCCTGTC GTGGTACGGC CCCACGTATG ATGGGGGCAG TGTGGTCGAG
    TCCTACTCGG TGGAGATCTG GGACTCAGTG GGCAAAGTCT GGACGGATCT GACCTCCTGC
    CGCAGCACCT CCTTTAATGT GGAGAATTTA CTGCAGGACA GAGAGTACAA ATTCCGGGTC
    CGGGCCACTA ACATCCACGG CACCAGCGAA CCCAGTCAGG AGTCGGAACC AGTTACCTAC
    AAGGAGAAGG AAGAAGAACA CAAAGAAGAA GAGGCAGAAG TTTCAGACGA CGAGGAGAAG
    GAGTCGGAGC CGGAATACAG GGACGTCACT CTCAACAGCA GCGAGAAAGT GACCGACTAC
    TACGATGTGG AGGAGAGACT GGGCACGGGC AAATTTGGCC AAGTCTTCCG AATCGTAGAG
    AAGAAGAGCA GGAAAATCTA TGCCGGGAAG TTCTTCAAAG CCTATTCGGC CAAAGACAAG
    GAGGGCATCC GGCAAGAAAT TGCCATCATG AACTGCCTGC ACCACCCCAA GCTGGTCCAG
    TGCGTCGACG CGTTTGAGTC CAAAGCCGAC ATGGTGATGG TTCTGGAACT GGTCTCGGGC
    GGGGAACTCT TCGAGCGAAT CATTGACGAG GATTTTGAGC TGACGGAGCG GGAGGTGATT
    AAGTACATGA AGCAGATCTG CGAGGGGGTC GAGTTCATCC ACAAACAGGG GATCGTGCAC
    CTCGACCTCA AACCCGAGAA CATCATGTGC GTCAATAAGA CTGGCACCAA GATCAAACTC
    ATCGACTTCG GCCTCGCCCG CAGATTGGAG AGCTGCCCGA ACCTCAAAGT GCTTTTTGGA
    ACCCCTGAAT TTGTAGCCCC CGAAGTGATA AACTACGAGC CGATTGGTTA CTCGACGGAC
    ATGTGGAGCA TCGGGGTCAT CTGCTACATC TTGGTCAGTG GACTTTCTCC ATTCATGGGG
    GATAATGACA ATGAAACGCT GGCGAATGTA ACTTCGGCCA CTTGGGACTT CGATGATGAA
    GCCTTTGATG AAATATCTGA AGAAGCCAAA AACTTAATCA GCAGTTTGTT GAAGAAGGAC
    ATGAAGAATC GGCTGAACTG TACCCAGTGC CTCCAGCATT CGTGGCTACA GCAGGACACT
    GCCACAATGG AAGCCAAGAA ACTCTCCAAA GACAGGATGA AGAAATATAT GGCTCGGAGG
    AAATGGCAGA AAACCGGGCA CGCAGTGAGG GCAATCGGAC GTCTCTCCTC TATGGCCATG
    ATCTCTGGGC TGAGCGCCAG GAAGTCATCG GGCAGCGCCC CCAGCAGCCC CCTGTCTGCG
    GAGCTTGAAG CCAATGAAGA AGACAACCAG GCTTTCCTGG AGTCGGTGAC AGAGGAGAAG
    CCCCCCGCAA AGCCTTTCTT CACGAAGACC ATTCTGGATA TCGAAGTCGT GGAAGGGAGC
    GCCGCGAGGT TTGACTGCAG AATTGAGGGG CACCCCGACC CGGAGGTGAT TTGGTACAAG
    GACGACCAAC CCATCAAGGA GTCTCGGCAC TTCCAGATCG ATTACGACGA GGACGGTAAC
    TGCTCCCTGA CCATCTCGGA AGTTTGCGGC GACGACGACG CCAAATACAC CTGCAAAGCC
    GCCAACAGCC TGGGGGATAC GACCTGCACG GCCGAGCTGA TTGTAGAGGT GATGGCGGAG
    GAGGAGGAGG AAGAGGAGGA GGAAGAGGAG GAGGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031749519.1
    CDS516..6374
    Translation

    Target ORF information:

    RefSeq Version XM_031893659.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis myosin light chain kinase (mylk), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031893659.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGTTTTTAT CGAAGGATAA AACCAGCGCC AAAGAACCTC CTTATCTTGG GGTGTCGGTT 
    CTAGACTCCA AGAACAAGAG CGTTCCACCA AAAGGCCCCG GAGACATCAT CCAAAAAGAG
    CCACTTGGAC GGACCCCGCG GGAACCCACC CAGGAGTTTA TAAGAGATTC CCCATCTGAT
    GGTTTCACAA AAGAGACGTC TTCCAAAAAG TTGCCTTCTG TAATGAACAG AGAGACCTCG
    TTCTTGTCGG CACGGGAGCG GATAATGGGG CCCACTATAA AGTCGCCTTA CGGTTCTGCT
    AAGACTTTAG GGAACCCGGC TTCTCTGTAT GAAATGTCTA AAGAGACCCG TTCAGATGTC
    CCTTCTAGAC AGATATCGAA CGTGACCCTC AGCGAGAGCT TGAAGCCAAA AGAGACCGCA
    GAATTTAGTT CTACGCAGTC TTTTCCCAAA TCTAGAGAAT CTCAATCTAG ACTCCATGGT
    ACTACAAGCA GCACCCTGTT GGGATCGAGC ACCCAGCAGC ACCCAGATAC CCCACAGCGT
    ACAGCCGAGA CCTCGCCAGA TGGTGGGGAG GTAAAACGCC AGAAGACCGC CTCCAGTACC
    ATCACCCTGA AGTCTGTCAA GATAGAAACC CAACCCAAGT CTGAGATAAG TCATGGTGGG
    ACCAGGCACA ACCAGCAGTC ACTTCCCTTG GGCCGGGAGG GGTCTCATTC ACACCCGGGG
    TTGTCGGACA CAAAAGAAAC CTTGAGCAGA AGCCTTGGAA CTGCCCCCGG CTTCGACCTG
    AGTCCCTCAA GCCAAGAGGT GCCGGAGGGC AGCGAAGTGA TATTCAGGTG CAAAGTTTCG
    GGAGATCCCA GACCGCGGGT CGAGTGGTTC CGAGAGAATT CGGCGCTGGG GGAGAGGGAC
    GGGGTGCGGA TCAGGGAAGG GGCTCGGATT CACTCGCTGC ACTTGCTGAA GGCACAGACG
    GGAGACAGCG GGAATTACAG CTGTGAAGTC TCCAACGCGA GAGGGCGGGT GTCTCATAGC
    TGGACCCTGA GCGTCACGCG GCGCAAGATG GAGGCGGTCG CGCCTCGGTT TGTCCGCAAG
    CTACGGGGCT GCTCCATTAT AGAGGGGGAG GATTTTGTGT TGGAATGTTC CATTGAGGGG
    CAACCGGCTC CTGAGATCTC GTGGCTGCTG AACGACCAGC CCATACAATA TGCCCACTCA
    GCGTATGAAG ACGGGGTGGC GAGGCTGCGG GTACAGGATG CCCTGCCGGA GGATGAGGGG
    GTATATACCT GCCTGGCACA GAACCGCGGC GGGAAGGTGT CCTGTTGTGC AACCGTCACT
    GTGACCGAAA AGAAGGCGGC AGAGGAGCCG GGGGGCGACC GAGAGCCAAA GAGCAGCCCC
    CCAATATTCC TGAAGCGCCT CACTGACCTG CAGGTTATGG ATGGGAGTCA AGTCACCATG
    AGCGTTGAAC TGTCAGGGAC CCCCCCTGCC GAGATCCTTT GGTTCCATAA TGGGAAAGAG
    ATTCAGGAGA CGGAGGATTT CCATTTTGAG CAGAGTGGTA ACGAGCACCG TCTGTGCATC
    CAGGAAGTCT TCCCCGAGGA CACAGGAACC TACATGTGCA AAGCCTGGAA TAGTTTGGGC
    CAAACTGCAA CGGAGGCCAC CTTAACCGTA CAAGAGCCGC AGGAGGGAAT CCAGCCATGG
    TTTATCAGCA AACCAAAGTG CACCAGCGCT TCAGTGGGGC AGAATGTGAT CCTATCCTGC
    GCCATCGCCG GGGACCCCTT CCCGCGGGTA CAATGGCTGA AGGACGGGCG CGAGTTGCCC
    CCCGAGCGGA TCCTGCAGAG CGACGACGTC TTCACTCTCA TCCTCAGAAA CGTCCAGCAG
    AGCGACGGCG GCCAATACCA GATCACGCTG ATCAATGCGG TGGGAGAATG CAGCTGCCGG
    GTCTCGCTGA TGGTCGAGGG AAGCTCCGGG GGTCCCTCTC ATACACACCA AACCTCCCTG
    ATTGTCTCTT TCTGTCTCTT TCCCAATCGG ACTCTCAGGC AGAGGGAGCA GCTGCGCTCT
    TGCGAGAGGG ATGGATGGGA TGAGGAGGAT GCTCTGAGTC TTGGCAGGAC TACTGAGGAG
    CAACAGGAGA ACATTCGAGG GGTTCTCAAG AGGAGAGTTG AGGTCAAGGA GAGCTCGGAG
    GAGAAGCTCC GGCAGCAAGA GGCGGAACAA GTCGACTTCC GTAGGGTTCT GGGCAAAAAG
    GTGAGCACGA GGAGCGTTTC CGAGGAGGAT TTGAAGGACA TCCCACCGGA GCAGATGGAC
    TTCCGAGCCA ACCTCCAGCG GCAGGTCAAA CCTAAGGCTG AGGATGAACG CAAGGTCAAT
    AGCCCACAGC AGGTGGACTT CCGCTCGGTA CTGGGCAAGA AGCCATCGAC TCCCAAAACC
    CCTCTTCCAG AGAAGGTGGT CGCCAAGGCA GCAACTCCTG ACTTCAGGTC GGTCCTCGGT
    GGCAAGAAAA AGCTTCCCAC TGAGAATGGC AGCCCCAACG ACGTTCCACA TGAACAGGGC
    AAATCAAAGG TGAACTGCTG CACGGTAGTG GATGGGGGGG TTGCCAAGAA CAGCGTGACC
    AACAACAGTG TCCCTAATGC AGCCAAGATC AGTGTCCTCA GTGTGGACAA GGCCAATGTC
    CCAGGTACAG CCAAGAACAG TGTCCCCAGT GTGGACAAGA CCAGTGCCCC AGGTACAGCC
    AAGAACAGTG TCCCCAGTGT GGACAAGACC AGTGCCCCAA GTACAGCCAA GAACAGTGTC
    TCAAGTGGAT CCAAGGCCAG TTTCCTAGGT GCGGATAAGG CCGGTGTCCC AAGTGTGGCC
    AAGACCAGTG TCCCAACTGC AACCAAGACC AATGTCCCAA GTGTGGACAA GACTAATGTC
    CCTGCCGCAA CCAAGACCAA TGTCCCAAGT TTGGCCAAGA CCAGTCTTCC AACTGCAACC
    AAGACCAATG TCCCAAGTGT GGACAAGGCC AGTGTCCCAA GTGTGGACAA GTCTAGTGTC
    CCTGCTGCAG TCAAGACCAA TGATAAGGAC GTTGCCCCAA GTGCAGACAA GCAGCTAACC
    CCAAATGTGG ATAAAGATAT TGCCCCAAGT TCAGAAAAGG ACGTTGCCCC AAGTGTGGAC
    AAGGACGTTG CCCCAAGTTC AGAGAAGGAC GTTGCCCCAA GTGTGGACAA GGACATTGCC
    CCAAGTGTGG ACAAGGACAT TGCCCCAAGT TCAGACAAAG ACGTTGCCCC CAGTTCAGAC
    AAGGATGTTG CCCCCAGTTC AGACAAGGAT GTTGCCCCCG GTTCAGACAA GGATGTTGCC
    CCCAGTTCAG ACAAGGATGT TGCCCCCAGT TCAGAGAAGG ACGTTGCCCC GAGTTCAGAG
    AAGGACGTTG CCCCAAATTC AGAGAAGGAC GTTGCCCCAA ATTCAGAGAA GGACGTTGCC
    CCAAGTTCAG AAAAGGACGT TGCCCCAAGT GCGCCCACAA AACCCGAGTT TTCGGAGGTA
    CTGCAGGATG TACGTGTTAC CGATGGGGAG AAGCTGGTGT TGCAGTGCCG CGTCACAGCC
    GAACCACCTC CGACCGTTGT GTGGACTCTG GATGGAAAAG TCATCAAGTC CTCCAAGTAC
    ATTGTCCTGT CCCAAGAAGG CTCCGTGTGT TCCCTGAGCA TCGAGAAGAC CTTCCCGGAG
    GACGAGGGGC AGTACCTGTG CGCGGTGGAG AATGCGGCCG GGAGGGCAGA ATGTGCCTGT
    AATGTCACGG TGGAAGATAC CTCGGGCAAC AAGGGCATCA AGAAGAGCAA GAAGCCGAGA
    AGCAACGCTC CGCTCTCCAA CGCAACGGAA ACATCTGCCC CTCAGATCCT ACAGTTCCCA
    GAAGACAGGA AGGTGAAAGC CGGGGAGTCG GTCGAGCTCC TGTGCAAGGT GGGGGGGACC
    CAGCCCATTA CCTGCATGTG GATGAAGTTC AGGAAGGAGA TTAAAGAAAG TGAATTTATC
    AAGATTGAGA GCACAGAAGA GACCAGTAAG CTGACAATCT CCTCGGCCCG ACAGGAGCAC
    TGCGGCTGCT ACACCCTGGT GGCGGAGAAC AAACAGGGCA GCAAACAGGC GCAGGTTAAC
    CTGACTGTGT TTGATAAACC CGACCCGCCG GCGGGGGTGC CCTGTGCCTC CTGTATGAAG
    GGCTCGGTGC TCACCCTGTC GTGGTACGGC CCCACGTATG ATGGGGGCAG TGTGGTCGAG
    TCCTACTCGG TGGAGATCTG GGACTCAGTG GGCAAAGTCT GGACGGATCT GACCTCCTGC
    CGCAGCACCT CCTTTAATGT GGAGAATTTA CTGCAGGACA GAGAGTACAA ATTCCGGGTC
    CGGGCCACTA ACATCCACGG CACCAGCGAA CCCAGTCAGG AGTCGGAACC AGTTACCTAC
    AAGGAGAAGG AAGAAGAACA CAAAGAAGAA GAGGCAGAAG TTTCAGACGA CGAGGAGAAG
    GAGTCGGAGC CGGAATACAG GGACGTCACT CTCAACAGCA GCGAGAAAGT GACCGACTAC
    TACGATGTGG AGGAGAGACT GGGCACGGGC AAATTTGGCC AAGTCTTCCG AATCGTAGAG
    AAGAAGAGCA GGAAAATCTA TGCCGGGAAG TTCTTCAAAG CCTATTCGGC CAAAGACAAG
    GAGGGCATCC GGCAAGAAAT TGCCATCATG AACTGCCTGC ACCACCCCAA GCTGGTCCAG
    TGCGTCGACG CGTTTGAGTC CAAAGCCGAC ATGGTGATGG TTCTGGAACT GGTCTCGGGC
    GGGGAACTCT TCGAGCGAAT CATTGACGAG GATTTTGAGC TGACGGAGCG GGAGGTGATT
    AAGTACATGA AGCAGATCTG CGAGGGGGTC GAGTTCATCC ACAAACAGGG GATCGTGCAC
    CTCGACCTCA AACCCGAGAA CATCATGTGC GTCAATAAGA CTGGCACCAA GATCAAACTC
    ATCGACTTCG GCCTCGCCCG CAGATTGGAG AGCTGCCCGA ACCTCAAAGT GCTTTTTGGA
    ACCCCTGAAT TTGTAGCCCC CGAAGTGATA AACTACGAGC CGATTGGTTA CTCGACGGAC
    ATGTGGAGCA TCGGGGTCAT CTGCTACATC TTGGTCAGTG GACTTTCTCC ATTCATGGGG
    GATAATGACA ATGAAACGCT GGCGAATGTA ACTTCGGCCA CTTGGGACTT CGATGATGAA
    GCCTTTGATG AAATATCTGA AGAAGCCAAA AACTTAATCA GCAGTTTGTT GAAGAAGGAC
    ATGAAGAATC GGCTGAACTG TACCCAGTGC CTCCAGCATT CGTGGCTACA GCAGGACACT
    GCCACAATGG AAGCCAAGAA ACTCTCCAAA GACAGGATGA AGAAATATAT GGCTCGGAGG
    AAATGGCAGA AAACCGGGCA CGCAGTGAGG GCAATCGGAC GTCTCTCCTC TATGGCCATG
    ATCTCTGGGC TGAGCGCCAG GAAGTCATCG GGCAGCGCCC CCAGCAGCCC CCTGTCTGCG
    GAGCTTGAAG CCAATGAAGA AGACAACCAG GCTTTCCTGG AGTCGGTGAC AGAGGAGAAG
    CCCCCCGCAA AGCCTTTCTT CACGAAGACC ATTCTGGATA TCGAAGTCGT GGAAGGGAGC
    GCCGCGAGGT TTGACTGCAG AATTGAGGGG CACCCCGACC CGGAGGTGAT TTGGTACAAG
    GACGACCAAC CCATCAAGGA GTCTCGGCAC TTCCAGATCG ATTACGACGA GGACGGTAAC
    TGCTCCCTGA CCATCTCGGA AGTTTGCGGC GACGACGACG CCAAATACAC CTGCAAAGCC
    GCCAACAGCC TGGGGGATAC GACCTGCACG GCCGAGCTGA TTGTAGAGGT GATGGCGGAG
    GAGGAGGAGG AAGAGGAGGA GGAAGAGGAG GAGGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.