sned1 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_002933337.5	XP_002933383.2	sushi, nidogen and EGF-like domain-containing protein 1 isoform X3
XM_012970833.3	XP_012826287.1	sushi, nidogen and EGF-like domain-containing protein 1 isoform X5
XM_012970835.2	XP_012826289.1	sushi, nidogen and EGF-like domain-containing protein 1 isoform X6
XM_012970829.3	XP_012826283.1	sushi, nidogen and EGF-like domain-containing protein 1 isoform X1
XM_012970829.2	XP_012826283.1	sushi, nidogen and EGF-like domain-containing protein 1 isoform X1
XM_012970834.2	XP_012826288.1	sushi, nidogen and EGF-like domain-containing protein 1 isoform X4
XM_012970835.1	XP_012826289.1	sushi, nidogen and EGF-like domain-containing protein 1 isoform X8
XM_012970833.2	XP_012826287.1	sushi, nidogen and EGF-like domain-containing protein 1 isoform X5
XM_018094449.1	XP_017949938.1	sushi, nidogen and EGF-like domain-containing protein 1 isoform X7
XM_012970831.3	XP_012826285.1	sushi, nidogen and EGF-like domain-containing protein 1 isoform X2
XM_012970836.1	XP_012826290.1	sushi, nidogen and EGF-like domain-containing protein 1 isoform X7
XM_012970831.2	XP_012826285.1	sushi, nidogen and EGF-like domain-containing protein 1 isoform X2
XM_002933337.4	XP_002933383.2	sushi, nidogen and EGF-like domain-containing protein 1 isoform X3
XM_018094448.1	XP_017949937.1	sushi, nidogen and EGF-like domain-containing protein 1 isoform X6
XM_031902549.1	XP_031758409.1	sushi, nidogen and EGF-like domain-containing protein 1 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa37193	XM_012970835.2 Latest version!	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa16046	XM_002933337.5 Latest version!	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa37188	XM_012970829.3 Latest version!	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa37189	XM_012970831.3 Latest version!	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa37190	XM_031902549.1 Latest version!	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa37191	XM_012970833.3 Latest version!	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa37192	XM_012970834.2 Latest version!	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa37194	XM_012970836.1 Latest version!	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OXa44795	XM_018094448.1 Latest version!	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa44796	XM_018094449.1 Latest version!	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
View Old Accession Versions >>
OXa37188	XM_012970829.2	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OXa37193	XM_012970835.1	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OXa37191	XM_012970833.2	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa37189	XM_012970831.2	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OXa16046	XM_002933337.4	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
Hide Old Accession Versions >>

sned1 ( XM_012970835.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826289.1 Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa37193
Clone ID Related Accession (Same CDS sequence)	XM_012970835.2 , XM_012970835.1
Accession Version	XM_012970835.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sushi, nidogen and EGF-like domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970835.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATGGA TCTGTTGCAC ATTCACTAAC ATGGGGAAAT GCTACTCCCA GCCCTGTCAG 
AATGACGGAA CCTGTGTGGA TGAGCCTGGA AGCTTCACCT GTCTGTGTAC CTCAGATTTT 
ACAGGAACCT TATGTGAGAC AGAGGTATCG CCCTGCACAT ATATGACCTG TCATAATGGT 
GGGGAGTGCG AGGATCATAA TGGAATTGCA GTATGCGTGT GTCAGCGAGG ATACACAGGT 
GACAACTGTG ATACAGAACT CTTGCCATGC TCTTCCTCTC CTTGTTTGAA TGGTGGGTCC 
TGCGAGAATA TTGATGCTGG GTTTCGCTGT CAGTGCGCTT TGGGATTTAC TGGAACACAC 
TGTGAAAAAG AACCCAGCGC ATGCTTTTCG TCTCCCTGTA TGAATAACGG AGTCTGCAAG 
GATGTGGGTG CTGGATACAT CTGTGTGTGT GTTCAGGGAT TCACTGGAAT GTATTGTGAA 
ACAGAGATCA GGCCATGTTC TTCAGCTCCA TGTTTAAACA GAGGTGTGTG CAGAGAGAAT 
GGCGAGACTT ACAGCTGTCA GTGTGCACGC CGATTCACCG GAAAGCATTG CGAGACAGAG 
ATCAGACCTT GTTTTTCTAA TCCCTGTATG AACATGGGGA CATGTGAGGA CATGAGTGAA 
GGTTATAGCT GTATCTGCAC AGAAGGTTAC ACTGGAATCC ACTGTGAGAC AGAGTTAAGA 
CCCTGCTATT CATCCCCTTG CCTTAATGGG GCATTGTGCA AGGACTTTGG CATTACTTAC 
AGCTGTGTGT GTGCATGGGG GTTCACTGGT GCACGATGTG AGACAGAGCT GCACCCATGC 
TTTTCTTCTC CTTGCCTGAA TGGAGCAACA TGCAGAGATG TCGGTTTTGC TTACAGCTGT 
CAGTGTGCAC AGGGATTCAC AGGGATGCAC TGTGAGACAG AGATCAAGCC ATGTTTTTCA 
TCCCCTTGTC TGAACGGAGG GACCTGTGAG GACCTGGCAA TCAGCTTTGC CTGTTTATGT 
GCAGAGAGAT ATACTGGCTT GCACTGCGAG ACAGAGATAC AGCCTTGCTT TTCCTCTCCA 
TGCCTGAATA GAGGAATCTG TAATAATGAA GGTGCATTTT ACAGCTGTAC ATGTGCAGAG 
GGCTTTACAG GAGAAAACTG TGAAAAAGAG GTGAGCCCTT GCCTTTCTTC TCCCTGTCTG 
AATGGAGCAA CCTGCATTGA CATGGGTGCA AGTTACAGCT GCATGTGCCC ACAGGCCTTC 
ACTGGGGCGC TCTGTGACAC GGAGATACCA AGCCCATGTA CATCAAATCC TTGTGAGAAC 
AAAGGTGTGT GCAGGGAGGC AGAAGGAGGC TACTTGTGTG ACTGCCCCGC AGGTTTCAGT 
GGCATGCACT GCGAGCACCG TGTGTCCGAT GACTGCCCCT GCAGGAATGG AGGGCGATGT 
GCAGATGGAA ACAGCACTTG TCACTGCCCG CCAGGACACT TTGGGCTTCT CTGTGAATAT 
GAGGTCACCT CGCTACCGTG CAGCATGGGT TCACAGTGTC CAGATGGCGG ATCCTGCATG 
GAATATGGTG GAAGTTACTT GTGTGTGTGT CACACAGATT ACATCCTTAG TAACCACTCA 
ATACCCTCAC CCTGTGACTC GGATCCCTGC CTGAATGGTG GAACATGTGA ATCTCTAGAT 
GATGCATATA CATGTGCCTG TCCCCGTGGC TTCAATGGAA GACTTTGTGA GAAAGTAAAG 
CCAGCTCTGT GCAGCCTATC ACCTTGCCGC AATGGGGGCA CCTGCAAGGA GTCACGAGAA 
GAATATTATT GCGTTTGTCC CTATCCATTC ACTGGAAAAC ACTGTGAGAC TGGTATGAGG 
GGCCCTTGTT CGTCAGGTCC GTGTAATAAT GGTGGTACAT GTTTCCATTA TCTTGGGAAA 
TACAAGTGCG ACTGCCCACC AAACTTCTCT GGGCGACACT GTGAGAAAGC AGAATTGGAC 
TGTGGAGCCC CAGAGCAGGT AAAATATGCG AAGATGATTG TGACATCTAC ACTGCCGGGG 
GGTCGAGCAG AATATCACTG TAATGAAGGC TATGTGCTCA GTACTCCAAA CAACACTAGT 
GTGTGCAGTG AGGAAGGGAC ATGGAGCTTG CCCCCAGAGT GTGAAGAAAT AGACGAGTGT 
GCATCTCATC CCTGCCATAA TGGGGCATCC TGCAAGGACC GGATTTCCCA CTTTATCTGT 
GAATGCCAGA AAGGGTACAG TGGAAAGTAT TGTGAGCAAG AAACTAATGA GTGTCTGTCA 
CAGCCATGTA AAAATGGGGG TACCTGTCAG GATTTGCCTG GGACCTTCAA ATGTATTTGT 
CCTGAGGGTT TCACTGGATC TTATTGTGAG ACAGAGGTGG ATGGATGTGA CTCTTCTCCG 
TGCCAGAATG GAGGCTTGTG TGAGAACCTG GCGGGTTCTT ATCTGTGCGT GTGTCCCCGC 
GGCTTTTTTG GCTATCACTG TCAGACAGTG AGTGACATGT GCCTGTTGAA CCCCTGCGGT 
AACCGTGGGG TATGTGTGTC CAGCGCAGAT GGATTGATGA GCTGCACCTG CAGAACGGGG 
TACACTGGAA CCATGTGTGA GAAAGAGCTG CTGCCGCCAT TGTCACTGTC TCTGGGTGCG 
GTTGGAGAAA ACAGTGTTTG GGTGTTCTGG ACCCCCCCAG AAGATGGCAC TGGGCATGTA 
CTGGATGGGT TTGCAGTCAG TTACACTGCC CCAGATGGCT TATCTAGAAC AGACTTTGTA 
GAAAGAGCTC ACACTGAACA CGAGCTGCGG GGGCTTAGTC CTGGCCGCTC TTATAACATC 
ACAATAGTAA CAGTAAAGCG CAACTTGCGG CACAATGATA TCAGTCGCCC CCTCTACCTC 
ACAGCACGAA CACGGCCACC TAACGTTGAC AGGCCGAGGA TTAATTCTGT CATGGACACA 
TCTGTCTCAG TTTCCTGGTC ACTAAGTCCT CACAGACACA CATCTATTGC TCGTGTTCGT 
CTGTTTCTCC AGCACCCAAC GGAGCCGCCC CAGCAGGCAG AGCAGGAGCC ATCAGCAGGA 
GAATATATCT TCAGGGATCT ACGTCCTGGA GAAAAATACA GCGTCCAAAT TAGCACCTTG 
AGCCGCTCTG ACCCCAGCCA CCCAGCCACA GAAAGCCTGC CCAGTGCACC TTTGATAGCC 
TGGACACGTC CACACCCACC TTCCAACCTT ACGGCAGCTC TTGTCACAGC CAATTCTGTG 
CATCTAATTT GGGAGCAACC TCCTCATGGG TCAGTGGATG GCTATATCTT CAATGTGACC 
AATAATCACA GTACAAAGAG CCGGTATGTT CCCAGTGGAA AGATGACTTC ATATACAGTC 
CGTGACTTGC AGGCCGGCCA ACGCTACCGG ATAACAGTGA CTGCACTACG CAACACTCCC 
CAGGGCCCAG TGAACAGTGA GCCCAAGACA GTGCGTCCAC AGACACTGCA AAGGGAGGGA 
CCTCAGGAGC GTCGTTGGAC CCCCCCTAGA ATTCTGCGGA ACAGGCCTCC TCCACAGCCA 
CATCAGGAGG TGCGACTGCT GACAGAAAGG GGTCCTCCTC CTGCTGATAC TCCACAGCCG 
CACCGATTCA CAGAGCTGAA CGATGGGCGC CGTCGTATTT CAGCAAGGTT CAGCCATCGT 
ACCAATAAAG CTATTAATGT TACAAAAGAG CCAGACCCTC CAGTCAAAAT TGAGAGCTCA 
GACGAAGCTG CTATTCCTGT GAGGCAGGCG CTGCAGCTAC AGGACCCTGA GCAAAGAAGC 
AAAGAAGGAG AACGACTGGG CTGCCTGGAG ACCCCGTGTC TTAATGGAGG GACCTGTGTG 
AATGGAGACA CTTGTGACTG TCCTCTGGGA TTTAAAGGGC AGCAATGCCA GTTGGCCTGC 
AGAAAGGAGA CCTTTTCCTG CACAAGACTG ATTTCTGAGA CAACATCACA CCCAGTGTGG 
GAGGGAGGAA TCTGCCACCA CCTGTACAGA AGAGTTTACA AGGTACAGAA TGATGTGTGC 
AGCAGGGAGG TTTGTGAAGG TACCAGCAGA AGCAGAGCAA GTGAGTGGAC TCTTTTGGTT 
ACAATTACAG AAAGCAGAAT CCCTTCCAAG CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826289.1
CDS	36..4151
Translation	MRWICCTFTNMGKCYSQPCQNDGTCVDEPGSFTCLCTSDFTGTLCETEVSPCTYMTCHNGGECEDHNGIAVCVCQRGYTGDNCDTELLPCSSSPCLNGGSCENIDAGFRCQCALGFTGTHCEKEPSACFSSPCMNNGVCKDVGAGYICVCVQGFTGMYCETEIRPCSSAPCLNRGVCRENGETYSCQCARRFTGKHCETEIRPCFSNPCMNMGTCEDMSEGYSCICTEGYTGIHCETELRPCYSSPCLNGALCKDFGITYSCVCAWGFTGARCETELHPCFSSPCLNGATCRDVGFAYSCQCAQGFTGMHCETEIKPCFSSPCLNGGTCEDLAISFACLCAERYTGLHCETEIQPCFSSPCLNRGICNNEGAFYSCTCAEGFTGENCEKEVSPCLSSPCLNGATCIDMGASYSCMCPQAFTGALCDTEIPSPCTSNPCENKGVCREAEGGYLCDCPAGFSGMHCEHRVSDDCPCRNGGRCADGNSTCHCPPGHFGLLCEYEVTSLPCSMGSQCPDGGSCMEYGGSYLCVCHTDYILSNHSIPSPCDSDPCLNGGTCESLDDAYTCACPRGFNGRLCEKVKPALCSLSPCRNGGTCKESREEYYCVCPYPFTGKHCETGMRGPCSSGPCNNGGTCFHYLGKYKCDCPPNFSGRHCEKAELDCGAPEQVKYAKMIVTSTLPGGRAEYHCNEGYVLSTPNNTSVCSEEGTWSLPPECEEIDECASHPCHNGASCKDRISHFICECQKGYSGKYCEQETNECLSQPCKNGGTCQDLPGTFKCICPEGFTGSYCETEVDGCDSSPCQNGGLCENLAGSYLCVCPRGFFGYHCQTVSDMCLLNPCGNRGVCVSSADGLMSCTCRTGYTGTMCEKELLPPLSLSLGAVGENSVWVFWTPPEDGTGHVLDGFAVSYTAPDGLSRTDFVERAHTEHELRGLSPGRSYNITIVTVKRNLRHNDISRPLYLTARTRPPNVDRPRINSVMDTSVSVSWSLSPHRHTSIARVRLFLQHPTEPPQQAEQEPSAGEYIFRDLRPGEKYSVQISTLSRSDPSHPATESLPSAPLIAWTRPHPPSNLTAALVTANSVHLIWEQPPHGSVDGYIFNVTNNHSTKSRYVPSGKMTSYTVRDLQAGQRYRITVTALRNTPQGPVNSEPKTVRPQTLQREGPQERRWTPPRILRNRPPPQPHQEVRLLTERGPPPADTPQPHRFTELNDGRRRISARFSHRTNKAINVTKEPDPPVKIESSDEAAIPVRQALQLQDPEQRSKEGERLGCLETPCLNGGTCVNGDTCDCPLGFKGQQCQLACRKETFSCTRLISETTSHPVWEGGICHHLYRRVYKVQNDVCSREVCEGTSRSRASEWTLLVTITESRIPSKP

Target ORF information:

RefSeq Version	XM_012970835.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970835.2

ORF Insert Sequence:

ATGAGATGGA TCTGTTGCAC ATTCACTAAC ATGGGGAAAT GCTACTCCCA GCCCTGTCAG 
AATGACGGAA CCTGTGTGGA TGAGCCTGGA AGCTTCACCT GTCTGTGTAC CTCAGATTTT 
ACAGGAACCT TATGTGAGAC AGAGGTATCG CCCTGCACAT ATATGACCTG TCATAATGGT 
GGGGAGTGCG AGGATCATAA TGGAATTGCA GTATGCGTGT GTCAGCGAGG ATACACAGGT 
GACAACTGTG ATACAGAACT CTTGCCATGC TCTTCCTCTC CTTGTTTGAA TGGTGGGTCC 
TGCGAGAATA TTGATGCTGG GTTTCGCTGT CAGTGCGCTT TGGGATTTAC TGGAACACAC 
TGTGAAAAAG AACCCAGCGC ATGCTTTTCG TCTCCCTGTA TGAATAACGG AGTCTGCAAG 
GATGTGGGTG CTGGATACAT CTGTGTGTGT GTTCAGGGAT TCACTGGAAT GTATTGTGAA 
ACAGAGATCA GGCCATGTTC TTCAGCTCCA TGTTTAAACA GAGGTGTGTG CAGAGAGAAT 
GGCGAGACTT ACAGCTGTCA GTGTGCACGC CGATTCACCG GAAAGCATTG CGAGACAGAG 
ATCAGACCTT GTTTTTCTAA TCCCTGTATG AACATGGGGA CATGTGAGGA CATGAGTGAA 
GGTTATAGCT GTATCTGCAC AGAAGGTTAC ACTGGAATCC ACTGTGAGAC AGAGTTAAGA 
CCCTGCTATT CATCCCCTTG CCTTAATGGG GCATTGTGCA AGGACTTTGG CATTACTTAC 
AGCTGTGTGT GTGCATGGGG GTTCACTGGT GCACGATGTG AGACAGAGCT GCACCCATGC 
TTTTCTTCTC CTTGCCTGAA TGGAGCAACA TGCAGAGATG TCGGTTTTGC TTACAGCTGT 
CAGTGTGCAC AGGGATTCAC AGGGATGCAC TGTGAGACAG AGATCAAGCC ATGTTTTTCA 
TCCCCTTGTC TGAACGGAGG GACCTGTGAG GACCTGGCAA TCAGCTTTGC CTGTTTATGT 
GCAGAGAGAT ATACTGGCTT GCACTGCGAG ACAGAGATAC AGCCTTGCTT TTCCTCTCCA 
TGCCTGAATA GAGGAATCTG TAATAATGAA GGTGCATTTT ACAGCTGTAC ATGTGCAGAG 
GGCTTTACAG GAGAAAACTG TGAAAAAGAG GTGAGCCCTT GCCTTTCTTC TCCCTGTCTG 
AATGGAGCAA CCTGCATTGA CATGGGTGCA AGTTACAGCT GCATGTGCCC ACAGGCCTTC 
ACTGGGGCGC TCTGTGACAC GGAGATACCA AGCCCATGTA CATCAAATCC TTGTGAGAAC 
AAAGGTGTGT GCAGGGAGGC AGAAGGAGGC TACTTGTGTG ACTGCCCCGC AGGTTTCAGT 
GGCATGCACT GCGAGCACCG TGTGTCCGAT GACTGCCCCT GCAGGAATGG AGGGCGATGT 
GCAGATGGAA ACAGCACTTG TCACTGCCCG CCAGGACACT TTGGGCTTCT CTGTGAATAT 
GAGGTCACCT CGCTACCGTG CAGCATGGGT TCACAGTGTC CAGATGGCGG ATCCTGCATG 
GAATATGGTG GAAGTTACTT GTGTGTGTGT CACACAGATT ACATCCTTAG TAACCACTCA 
ATACCCTCAC CCTGTGACTC GGATCCCTGC CTGAATGGTG GAACATGTGA ATCTCTAGAT 
GATGCATATA CATGTGCCTG TCCCCGTGGC TTCAATGGAA GACTTTGTGA GAAAGTAAAG 
CCAGCTCTGT GCAGCCTATC ACCTTGCCGC AATGGGGGCA CCTGCAAGGA GTCACGAGAA 
GAATATTATT GCGTTTGTCC CTATCCATTC ACTGGAAAAC ACTGTGAGAC TGGTATGAGG 
GGCCCTTGTT CGTCAGGTCC GTGTAATAAT GGTGGTACAT GTTTCCATTA TCTTGGGAAA 
TACAAGTGCG ACTGCCCACC AAACTTCTCT GGGCGACACT GTGAGAAAGC AGAATTGGAC 
TGTGGAGCCC CAGAGCAGGT AAAATATGCG AAGATGATTG TGACATCTAC ACTGCCGGGG 
GGTCGAGCAG AATATCACTG TAATGAAGGC TATGTGCTCA GTACTCCAAA CAACACTAGT 
GTGTGCAGTG AGGAAGGGAC ATGGAGCTTG CCCCCAGAGT GTGAAGAAAT AGACGAGTGT 
GCATCTCATC CCTGCCATAA TGGGGCATCC TGCAAGGACC GGATTTCCCA CTTTATCTGT 
GAATGCCAGA AAGGGTACAG TGGAAAGTAT TGTGAGCAAG AAACTAATGA GTGTCTGTCA 
CAGCCATGTA AAAATGGGGG TACCTGTCAG GATTTGCCTG GGACCTTCAA ATGTATTTGT 
CCTGAGGGTT TCACTGGATC TTATTGTGAG ACAGAGGTGG ATGGATGTGA CTCTTCTCCG 
TGCCAGAATG GAGGCTTGTG TGAGAACCTG GCGGGTTCTT ATCTGTGCGT GTGTCCCCGC 
GGCTTTTTTG GCTATCACTG TCAGACAGTG AGTGACATGT GCCTGTTGAA CCCCTGCGGT 
AACCGTGGGG TATGTGTGTC CAGCGCAGAT GGATTGATGA GCTGCACCTG CAGAACGGGG 
TACACTGGAA CCATGTGTGA GAAAGAGCTG CTGCCGCCAT TGTCACTGTC TCTGGGTGCG 
GTTGGAGAAA ACAGTGTTTG GGTGTTCTGG ACCCCCCCAG AAGATGGCAC TGGGCATGTA 
CTGGATGGGT TTGCAGTCAG TTACACTGCC CCAGATGGCT TATCTAGAAC AGACTTTGTA 
GAAAGAGCTC ACACTGAACA CGAGCTGCGG GGGCTTAGTC CTGGCCGCTC TTATAACATC 
ACAATAGTAA CAGTAAAGCG CAACTTGCGG CACAATGATA TCAGTCGCCC CCTCTACCTC 
ACAGCACGAA CACGGCCACC TAACGTTGAC AGGCCGAGGA TTAATTCTGT CATGGACACA 
TCTGTCTCAG TTTCCTGGTC ACTAAGTCCT CACAGACACA CATCTATTGC TCGTGTTCGT 
CTGTTTCTCC AGCACCCAAC GGAGCCGCCC CAGCAGGCAG AGCAGGAGCC ATCAGCAGGA 
GAATATATCT TCAGGGATCT ACGTCCTGGA GAAAAATACA GCGTCCAAAT TAGCACCTTG 
AGCCGCTCTG ACCCCAGCCA CCCAGCCACA GAAAGCCTGC CCAGTGCACC TTTGATAGCC 
TGGACACGTC CACACCCACC TTCCAACCTT ACGGCAGCTC TTGTCACAGC CAATTCTGTG 
CATCTAATTT GGGAGCAACC TCCTCATGGG TCAGTGGATG GCTATATCTT CAATGTGACC 
AATAATCACA GTACAAAGAG CCGGTATGTT CCCAGTGGAA AGATGACTTC ATATACAGTC 
CGTGACTTGC AGGCCGGCCA ACGCTACCGG ATAACAGTGA CTGCACTACG CAACACTCCC 
CAGGGCCCAG TGAACAGTGA GCCCAAGACA GTGCGTCCAC AGACACTGCA AAGGGAGGGA 
CCTCAGGAGC GTCGTTGGAC CCCCCCTAGA ATTCTGCGGA ACAGGCCTCC TCCACAGCCA 
CATCAGGAGG TGCGACTGCT GACAGAAAGG GGTCCTCCTC CTGCTGATAC TCCACAGCCG 
CACCGATTCA CAGAGCTGAA CGATGGGCGC CGTCGTATTT CAGCAAGGTT CAGCCATCGT 
ACCAATAAAG CTATTAATGT TACAAAAGAG CCAGACCCTC CAGTCAAAAT TGAGAGCTCA 
GACGAAGCTG CTATTCCTGT GAGGCAGGCG CTGCAGCTAC AGGACCCTGA GCAAAGAAGC 
AAAGAAGGAG AACGACTGGG CTGCCTGGAG ACCCCGTGTC TTAATGGAGG GACCTGTGTG 
AATGGAGACA CTTGTGACTG TCCTCTGGGA TTTAAAGGGC AGCAATGCCA GTTGGCCTGC 
AGAAAGGAGA CCTTTTCCTG CACAAGACTG ATTTCTGAGA CAACATCACA CCCAGTGTGG 
GAGGGAGGAA TCTGCCACCA CCTGTACAGA AGAGTTTACA AGGTACAGAA TGATGTGTGC 
AGCAGGGAGG TTTGTGAAGG TACCAGCAGA AGCAGAGCAA GTGAGTGGAC TCTTTTGGTT 
ACAATTACAG AAAGCAGAAT CCCTTCCAAG CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sned1 ( XM_002933337.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933383.2 Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa16046
Clone ID Related Accession (Same CDS sequence)	XM_002933337.5 , XM_002933337.4
Accession Version	XM_002933337.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sushi, nidogen and EGF-like domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002933337.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA 
GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA 
TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG 
GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC 
TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA 
GGGTACAGTG GAAAGTATTG TGAGCAAGAA ACTAATGAGT GTCTGTCACA GCCATGTAAA 
AATGGGGGTA CCTGTCAGGA TTTGCCTGGG ACCTTCAAAT GTATTTGTCC TGAGGGTTTC 
ACTGGATCTT ATTGTGAGAC AGAGGTGGAT GGATGTGACT CTTCTCCGTG CCAGAATGGA 
GGCTTGTGTG AGAACCTGGC GGGTTCTTAT CTGTGCGTGT GTCCCCGCGG CTTTTTTGGC 
TATCACTGTC AGACAGTGAG TGACATGTGC CTGTTGAACC CCTGCGGTAA CCGTGGGGTA 
TGTGTGTCCA GCGCAGATGG ATTGATGAGC TGCACCTGCA GAACGGGGTA CACTGGAACC 
ATGTGTGAGA AAGAGCTGCT GCCGCCATTG TCACTGTCTC TGGGTGCGGT TGGAGAAAAC 
AGTGTTTGGG TGTTCTGGAC CCCCCCAGAA GATGGCACTG GGCATGTACT GGATGGGTTT 
GCAGTCAGTT ACACTGCCCC AGATGGCTTA TCTAGAACAG ACTTTGTAGA AAGAGCTCAC 
ACTGAACACG AGCTGCGGGG GCTTAGTCCT GGCCGCTCTT ATAACATCAC AATAGTAACA 
GTAAAGCGCA ACTTGCGGCA CAATGATATC AGTCGCCCCC TCTACCTCAC AGCACGAACA 
CGGCCACCTA ACGTTGACAG GCCGAGGATT AATTCTGTCA TGGACACATC TGTCTCAGTT 
TCCTGGTCAC TAAGTCCTCA CAGACACACA TCTATTGCTC GTGTTCGTCT GTTTCTCCAG 
CACCCAACGG AGCCGCCCCA GCAGGCAGAG CAGGAGCCAT CAGCAGGAGA ATATATCTTC 
AGGGATCTAC GTCCTGGAGA AAAATACAGC GTCCAAATTA GCACCTTGAG CCGCTCTGAC 
CCCAGCCACC CAGCCACAGA AAGCCTGCCC AGTGCACCTT TGATAGCCTG GACACGTCCA 
CACCCACCTT CCAACCTTAC GGCAGCTCTT GTCACAGCCA ATTCTGTGCA TCTAATTTGG 
GAGCAACCTC CTCATGGGTC AGTGGATGGC TATATCTTCA ATGTGACCAA TAATCACAGT 
ACAAAGAGCC GGTATGTTCC CAGTGGAAAG ATGACTTCAT ATACAGTCCG TGACTTGCAG 
GCCGGCCAAC GCTACCGGAT AACAGTGACT GCACTACGCA ACACTCCCCA GGGCCCAGTG 
AACAGTGAGC CCAAGACAGT GCGTCCACAG ACACTGCAAA GGGAGGGACC TCAGGAGCGT 
CGTTGGACCC CCCCTAGAAT TCTGCGGAAC AGGCCTCCTC CACAGCCACA TCAGGAGGTG 
CGACTGCTGA CAGAAAGGGG TCCTCCTCCT GCTGATACTC CACAGCCGCA CCGATTCACA 
GAGCTGAACG ATGGGCGCCG TCGTATTTCA GCAAGGTTCA GCCATCGTAC CAATAAAGCT 
ATTAATGTTA CAAAAGAGCC AGACCCTCCA GTCAAAATTG AGAGCTCAGA CGAAGCTGCT 
ATTCCTGTGA GGCAGGCGCT GCAGCTACAG GACCCTGAGC AAAGAAGCAA AGAAGGAGAA 
CGACTGGGCT GCCTGGAGAC CCCGTGTCTT AATGGAGGGA CCTGTGTGAA TGGAGACACT 
TGTGACTGTC CTCTGGGATT TAAAGGGCAG CAATGCCAGT TGGCCTGCAG AAAGGAGACC 
TTTTCCTGCA CAAGACTGAT TTCTGAGACA ACATCACACC CAGTGTGGGA GGGAGGAATC 
TGCCACCACC TGTACAGAAG AGTTTACAAG GTACAGAATG ATGTGTGCAG CAGGGAGGTT 
TGTGAAGGTA CCAGCAGAAG CAGAGCAAAA AGCAGAATCC CTTCCAAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933383.2
CDS	1288..6261
Translation	MRLPSLFWIFISMVWRAQGMVPLEDFYPFGTKEGDTVTRRQDDGGSGLLPISKAFTFFGDKHSGLYVNNNGVVSFLREVSQFTPVAFPISGDRRVVAAFWADVDNRRGGNVFYRETEDLVIVDRATRDVRTYFPELPDFHSTWVFIATWHRVTFYGGDSTSPVNTFQIVLITDGLRSFTIFNYETIEWTTGRHASSGGDSSGRGGIAAQAGFNAGDGWRYFNIPGSRTDDIARIANTTNVGVPGRWVFRIDNNHVQVGECRNATSVCWTLRPCQNGGRCIDDCVTGNPSYTCSCLSGFTGKHCHIDMGKCYSQPCQNDGTCVDEPGSFTCLCTSDFTGTLCETEVSPCTYMTCHNGGECEDHNGIAVCVCQRGYTGDNCDTELLPCSSSPCLNGGSCENIDAGFRCQCALGFTGTHCEKEPSACFSSPCMNNGVCKDVGAGYICVCVQGFTGMYCETEIRPCSSAPCLNRGVCRENGETYSCQCARRFTGKHCETEIRPCFSNPCMNMGTCEDMSEGYSCICTEGYTGIHCETELRPCYSSPCLNGALCKDFGITYSCVCAWGFTGARCETELHPCFSSPCLNGATCRDVGFAYSCQCAQGFTGMHCETEIKPCFSSPCLNGGTCEDLAISFACLCAERYTGLHCETEIQPCFSSPCLNRGICNNEGAFYSCTCAEGFTGENCEKEVSPCLSSPCLNGATCIDMGASYSCMCPQAFTGALCDTEIPSPCTSNPCENKGVCREAEGGYLCDCPAGFSGMHCEHRVSDDCPCRNGGRCADGNSTCHCPPGHFGLLCEYEVTSLPCSMGSQCPDGGSCMEYGGSYLCVCHTDYILSNHSIPSPCDSDPCLNGGTCESLDDAYTCACPRGFNGRLCEKVKPALCSLSPCRNGGTCKESREEYYCVCPYPFTGKHCETGMRGPCSSGPCNNGGTCFHYLGKYKCDCPPNFSGRHCEKAELDCGAPEQVKYAKMIVTSTLPGGRAEYHCNEGYVLSTPNNTSVCSEEGTWSLPPECEEIDECASHPCHNGASCKDRISHFICECQKGYSGKYCEQETNECLSQPCKNGGTCQDLPGTFKCICPEGFTGSYCETEVDGCDSSPCQNGGLCENLAGSYLCVCPRGFFGYHCQTVSDMCLLNPCGNRGVCVSSADGLMSCTCRTGYTGTMCEKELLPPLSLSLGAVGENSVWVFWTPPEDGTGHVLDGFAVSYTAPDGLSRTDFVERAHTEHELRGLSPGRSYNITIVTVKRNLRHNDISRPLYLTARTRPPNVDRPRINSVMDTSVSVSWSLSPHRHTSIARVRLFLQHPTEPPQQAEQEPSAGEYIFRDLRPGEKYSVQISTLSRSDPSHPATESLPSAPLIAWTRPHPPSNLTAALVTANSVHLIWEQPPHGSVDGYIFNVTNNHSTKSRYVPSGKMTSYTVRDLQAGQRYRITVTALRNTPQGPVNSEPKTVRPQTLQREGPQERRWTPPRILRNRPPPQPHQEVRLLTERGPPPADTPQPHRFTELNDGRRRISARFSHRTNKAINVTKEPDPPVKIESSDEAAIPVRQALQLQDPEQRSKEGERLGCLETPCLNGGTCVNGDTCDCPLGFKGQQCQLACRKETFSCTRLISETTSHPVWEGGICHHLYRRVYKVQNDVCSREVCEGTSRSRAKSRIPSKP

Target ORF information:

RefSeq Version	XM_002933337.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933337.5

ORF Insert Sequence:

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA 
GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA 
TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG 
GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC 
TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA 
GGGTACAGTG GAAAGTATTG TGAGCAAGAA ACTAATGAGT GTCTGTCACA GCCATGTAAA 
AATGGGGGTA CCTGTCAGGA TTTGCCTGGG ACCTTCAAAT GTATTTGTCC TGAGGGTTTC 
ACTGGATCTT ATTGTGAGAC AGAGGTGGAT GGATGTGACT CTTCTCCGTG CCAGAATGGA 
GGCTTGTGTG AGAACCTGGC GGGTTCTTAT CTGTGCGTGT GTCCCCGCGG CTTTTTTGGC 
TATCACTGTC AGACAGTGAG TGACATGTGC CTGTTGAACC CCTGCGGTAA CCGTGGGGTA 
TGTGTGTCCA GCGCAGATGG ATTGATGAGC TGCACCTGCA GAACGGGGTA CACTGGAACC 
ATGTGTGAGA AAGAGCTGCT GCCGCCATTG TCACTGTCTC TGGGTGCGGT TGGAGAAAAC 
AGTGTTTGGG TGTTCTGGAC CCCCCCAGAA GATGGCACTG GGCATGTACT GGATGGGTTT 
GCAGTCAGTT ACACTGCCCC AGATGGCTTA TCTAGAACAG ACTTTGTAGA AAGAGCTCAC 
ACTGAACACG AGCTGCGGGG GCTTAGTCCT GGCCGCTCTT ATAACATCAC AATAGTAACA 
GTAAAGCGCA ACTTGCGGCA CAATGATATC AGTCGCCCCC TCTACCTCAC AGCACGAACA 
CGGCCACCTA ACGTTGACAG GCCGAGGATT AATTCTGTCA TGGACACATC TGTCTCAGTT 
TCCTGGTCAC TAAGTCCTCA CAGACACACA TCTATTGCTC GTGTTCGTCT GTTTCTCCAG 
CACCCAACGG AGCCGCCCCA GCAGGCAGAG CAGGAGCCAT CAGCAGGAGA ATATATCTTC 
AGGGATCTAC GTCCTGGAGA AAAATACAGC GTCCAAATTA GCACCTTGAG CCGCTCTGAC 
CCCAGCCACC CAGCCACAGA AAGCCTGCCC AGTGCACCTT TGATAGCCTG GACACGTCCA 
CACCCACCTT CCAACCTTAC GGCAGCTCTT GTCACAGCCA ATTCTGTGCA TCTAATTTGG 
GAGCAACCTC CTCATGGGTC AGTGGATGGC TATATCTTCA ATGTGACCAA TAATCACAGT 
ACAAAGAGCC GGTATGTTCC CAGTGGAAAG ATGACTTCAT ATACAGTCCG TGACTTGCAG 
GCCGGCCAAC GCTACCGGAT AACAGTGACT GCACTACGCA ACACTCCCCA GGGCCCAGTG 
AACAGTGAGC CCAAGACAGT GCGTCCACAG ACACTGCAAA GGGAGGGACC TCAGGAGCGT 
CGTTGGACCC CCCCTAGAAT TCTGCGGAAC AGGCCTCCTC CACAGCCACA TCAGGAGGTG 
CGACTGCTGA CAGAAAGGGG TCCTCCTCCT GCTGATACTC CACAGCCGCA CCGATTCACA 
GAGCTGAACG ATGGGCGCCG TCGTATTTCA GCAAGGTTCA GCCATCGTAC CAATAAAGCT 
ATTAATGTTA CAAAAGAGCC AGACCCTCCA GTCAAAATTG AGAGCTCAGA CGAAGCTGCT 
ATTCCTGTGA GGCAGGCGCT GCAGCTACAG GACCCTGAGC AAAGAAGCAA AGAAGGAGAA 
CGACTGGGCT GCCTGGAGAC CCCGTGTCTT AATGGAGGGA CCTGTGTGAA TGGAGACACT 
TGTGACTGTC CTCTGGGATT TAAAGGGCAG CAATGCCAGT TGGCCTGCAG AAAGGAGACC 
TTTTCCTGCA CAAGACTGAT TTCTGAGACA ACATCACACC CAGTGTGGGA GGGAGGAATC 
TGCCACCACC TGTACAGAAG AGTTTACAAG GTACAGAATG ATGTGTGCAG CAGGGAGGTT 
TGTGAAGGTA CCAGCAGAAG CAGAGCAAAA AGCAGAATCC CTTCCAAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sned1 ( XM_012970829.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826283.1 Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X1, mRNA.

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CloneID	OXa37188
Clone ID Related Accession (Same CDS sequence)	XM_012970829.3 , XM_012970829.2
Accession Version	XM_012970829.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sushi, nidogen and EGF-like domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970829.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA 
GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA 
TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG 
GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC 
TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA 
GGGTACAGTG GAAAGTATTG TGAGCAAGAA ACTAATGAGT GTCTGTCACA GCCATGTAAA 
AATGGGGGTA CCTGTCAGGA TTTGCCTGGG ACCTTCAAAT GTATTTGTCC TGAGGGTTTC 
ACTGGATCTT ATTGTGAGAC AGAGGTGGAT GGATGTGACT CTTCTCCGTG CCAGAATGGA 
GGCTTGTGTG AGAACCTGGC GGGTTCTTAT CTGTGCGTGT GTCCCCGCGG CTTTTTTGGC 
TATCACTGTC AGACAGTGAG TGACATGTGC CTGTTGAACC CCTGCGGTAA CCGTGGGGTA 
TGTGTGTCCA GCGCAGATGG ATTGATGAGC TGCACCTGCA GAACGGGGTA CACTGGAACC 
ATGTGTGAGA AAGAGCTGCT GCCGCCATTG TCACTGTCTC TGGGTGCGGT TGGAGAAAAC 
AGTGTTTGGG TGTTCTGGAC CCCCCCAGAA GATGGCACTG GGCATGTACT GGATGGGTTT 
GCAGTCAGTT ACACTGCCCC AGATGGCTTA TCTAGAACAG ACTTTGTAGA AAGAGCTCAC 
ACTGAACACG AGCTGCGGGG GCTTAGTCCT GGCCGCTCTT ATAACATCAC AATAGTAACA 
GTAAAGCGCA ACTTGCGGCA CAATGATATC AGTCGCCCCC TCTACCTCAC AGCACGAACA 
CGGCCACCTA ACGTTGACAG GCCGAGGATT AATTCTGTCA TGGACACATC TGTCTCAGTT 
TCCTGGTCAC TAAGTCCTCA CAGACACACA TCTATTGCTC GTGTTCGTCT GTTTCTCCAG 
CACCCAACGG AGCCGCCCCA GCAGGCAGAG CAGGAGCCAT CAGCAGGAGA ATATATCTTC 
AGGGATCTAC GTCCTGGAGA AAAATACAGC GTCCAAATTA GCACCTTGAG CCGCTCTGAC 
CCCAGCCACC CAGCCACAGA AAGCCTGCCC AGTGCACCTT TGATAGCCTG GACACGTCCA 
CACCCACCTT CCAACCTTAC GGCAGCTCTT GTCACAGCCA ATTCTGTGCA TCTAATTTGG 
GAGCAACCTC CTCATGGGTC AGTGGATGGC TATATCTTCA ATGTGACCAA TAATCACAGT 
ACAAAGAGCC GGTATGTTCC CAGTGGAAAG ATGACTTCAT ATACAGTCCG TGACTTGCAG 
GCCGGCCAAC GCTACCGGAT AACAGTGACT GCACTACGCA ACACTCCCCA GGGCCCAGTG 
AACAGTGAGC CCAAGACAGT GCGTCCACAG ACACTGCAAA GGGAGGGACC TCAGGAGCGT 
CGTTGGACCC CCCCTAGAAT TCTGCGGAAC AGGCCTCCTC CACAGCCACA TCAGGAGGTG 
CGACTGCTGA CAGAAAGGGG TCCTCCTCCT GCTGATACTC CACAGCCGCA CCGATTCACA 
GAGCTGAACG ATGGGCGCCG TCGTATTTCA GCAAGGTTCA GCCATCGTAC CAATAAAGCT 
ATTAATGTTA CAAAAGAGCC AGACCCTCCA GTCAAAATTG AGAGCTCAGA CGAAGCTGCT 
ATTCCTGTGA GGCAGGCGCT GCAGCTACAG GACCCTGAGC AAAGAAGCAA AGAAGGAGAA 
CGACTGGGCT GCCTGGAGAC CCCGTGTCTT AATGGAGGGA CCTGTGTGAA TGGAGACACT 
TGTGACTGTC CTCTGGGATT TAAAGGGCAG CAATGCCAGT TGGCCTGCAG AAAGGAGACC 
TTTTCCTGCA CAAGACTGAT TTCTGAGACA ACATCACACC CAGTGTGGGA GGGAGGAATC 
TGCCACCACC TGTACAGAAG AGTTTACAAG GTACAGAATG ATGTGTGCAG CAGGGAGGTT 
TGTGAAGGTA CCAGCAGAAG CAGAGCAAGT GAGTGGACTC TTTTGGTTAC AATTACAGAA 
AGCAGAATCC CTTCCAAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826283.1
CDS	1294..6297
Translation	MRLPSLFWIFISMVWRAQGMVPLEDFYPFGTKEGDTVTRRQDDGGSGLLPISKAFTFFGDKHSGLYVNNNGVVSFLREVSQFTPVAFPISGDRRVVAAFWADVDNRRGGNVFYRETEDLVIVDRATRDVRTYFPELPDFHSTWVFIATWHRVTFYGGDSTSPVNTFQIVLITDGLRSFTIFNYETIEWTTGRHASSGGDSSGRGGIAAQAGFNAGDGWRYFNIPGSRTDDIARIANTTNVGVPGRWVFRIDNNHVQVGECRNATSVCWTLRPCQNGGRCIDDCVTGNPSYTCSCLSGFTGKHCHIDMGKCYSQPCQNDGTCVDEPGSFTCLCTSDFTGTLCETEVSPCTYMTCHNGGECEDHNGIAVCVCQRGYTGDNCDTELLPCSSSPCLNGGSCENIDAGFRCQCALGFTGTHCEKEPSACFSSPCMNNGVCKDVGAGYICVCVQGFTGMYCETEIRPCSSAPCLNRGVCRENGETYSCQCARRFTGKHCETEIRPCFSNPCMNMGTCEDMSEGYSCICTEGYTGIHCETELRPCYSSPCLNGALCKDFGITYSCVCAWGFTGARCETELHPCFSSPCLNGATCRDVGFAYSCQCAQGFTGMHCETEIKPCFSSPCLNGGTCEDLAISFACLCAERYTGLHCETEIQPCFSSPCLNRGICNNEGAFYSCTCAEGFTGENCEKEVSPCLSSPCLNGATCIDMGASYSCMCPQAFTGALCDTEIPSPCTSNPCENKGVCREAEGGYLCDCPAGFSGMHCEHRVSDDCPCRNGGRCADGNSTCHCPPGHFGLLCEYEVTSLPCSMGSQCPDGGSCMEYGGSYLCVCHTDYILSNHSIPSPCDSDPCLNGGTCESLDDAYTCACPRGFNGRLCEKVKPALCSLSPCRNGGTCKESREEYYCVCPYPFTGKHCETGMRGPCSSGPCNNGGTCFHYLGKYKCDCPPNFSGRHCEKAELDCGAPEQVKYAKMIVTSTLPGGRAEYHCNEGYVLSTPNNTSVCSEEGTWSLPPECEEIDECASHPCHNGASCKDRISHFICECQKGYSGKYCEQETNECLSQPCKNGGTCQDLPGTFKCICPEGFTGSYCETEVDGCDSSPCQNGGLCENLAGSYLCVCPRGFFGYHCQTVSDMCLLNPCGNRGVCVSSADGLMSCTCRTGYTGTMCEKELLPPLSLSLGAVGENSVWVFWTPPEDGTGHVLDGFAVSYTAPDGLSRTDFVERAHTEHELRGLSPGRSYNITIVTVKRNLRHNDISRPLYLTARTRPPNVDRPRINSVMDTSVSVSWSLSPHRHTSIARVRLFLQHPTEPPQQAEQEPSAGEYIFRDLRPGEKYSVQISTLSRSDPSHPATESLPSAPLIAWTRPHPPSNLTAALVTANSVHLIWEQPPHGSVDGYIFNVTNNHSTKSRYVPSGKMTSYTVRDLQAGQRYRITVTALRNTPQGPVNSEPKTVRPQTLQREGPQERRWTPPRILRNRPPPQPHQEVRLLTERGPPPADTPQPHRFTELNDGRRRISARFSHRTNKAINVTKEPDPPVKIESSDEAAIPVRQALQLQDPEQRSKEGERLGCLETPCLNGGTCVNGDTCDCPLGFKGQQCQLACRKETFSCTRLISETTSHPVWEGGICHHLYRRVYKVQNDVCSREVCEGTSRSRASEWTLLVTITESRIPSKP

Target ORF information:

RefSeq Version	XM_012970829.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970829.3

ORF Insert Sequence:

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA 
GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA 
TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG 
GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC 
TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA 
GGGTACAGTG GAAAGTATTG TGAGCAAGAA ACTAATGAGT GTCTGTCACA GCCATGTAAA 
AATGGGGGTA CCTGTCAGGA TTTGCCTGGG ACCTTCAAAT GTATTTGTCC TGAGGGTTTC 
ACTGGATCTT ATTGTGAGAC AGAGGTGGAT GGATGTGACT CTTCTCCGTG CCAGAATGGA 
GGCTTGTGTG AGAACCTGGC GGGTTCTTAT CTGTGCGTGT GTCCCCGCGG CTTTTTTGGC 
TATCACTGTC AGACAGTGAG TGACATGTGC CTGTTGAACC CCTGCGGTAA CCGTGGGGTA 
TGTGTGTCCA GCGCAGATGG ATTGATGAGC TGCACCTGCA GAACGGGGTA CACTGGAACC 
ATGTGTGAGA AAGAGCTGCT GCCGCCATTG TCACTGTCTC TGGGTGCGGT TGGAGAAAAC 
AGTGTTTGGG TGTTCTGGAC CCCCCCAGAA GATGGCACTG GGCATGTACT GGATGGGTTT 
GCAGTCAGTT ACACTGCCCC AGATGGCTTA TCTAGAACAG ACTTTGTAGA AAGAGCTCAC 
ACTGAACACG AGCTGCGGGG GCTTAGTCCT GGCCGCTCTT ATAACATCAC AATAGTAACA 
GTAAAGCGCA ACTTGCGGCA CAATGATATC AGTCGCCCCC TCTACCTCAC AGCACGAACA 
CGGCCACCTA ACGTTGACAG GCCGAGGATT AATTCTGTCA TGGACACATC TGTCTCAGTT 
TCCTGGTCAC TAAGTCCTCA CAGACACACA TCTATTGCTC GTGTTCGTCT GTTTCTCCAG 
CACCCAACGG AGCCGCCCCA GCAGGCAGAG CAGGAGCCAT CAGCAGGAGA ATATATCTTC 
AGGGATCTAC GTCCTGGAGA AAAATACAGC GTCCAAATTA GCACCTTGAG CCGCTCTGAC 
CCCAGCCACC CAGCCACAGA AAGCCTGCCC AGTGCACCTT TGATAGCCTG GACACGTCCA 
CACCCACCTT CCAACCTTAC GGCAGCTCTT GTCACAGCCA ATTCTGTGCA TCTAATTTGG 
GAGCAACCTC CTCATGGGTC AGTGGATGGC TATATCTTCA ATGTGACCAA TAATCACAGT 
ACAAAGAGCC GGTATGTTCC CAGTGGAAAG ATGACTTCAT ATACAGTCCG TGACTTGCAG 
GCCGGCCAAC GCTACCGGAT AACAGTGACT GCACTACGCA ACACTCCCCA GGGCCCAGTG 
AACAGTGAGC CCAAGACAGT GCGTCCACAG ACACTGCAAA GGGAGGGACC TCAGGAGCGT 
CGTTGGACCC CCCCTAGAAT TCTGCGGAAC AGGCCTCCTC CACAGCCACA TCAGGAGGTG 
CGACTGCTGA CAGAAAGGGG TCCTCCTCCT GCTGATACTC CACAGCCGCA CCGATTCACA 
GAGCTGAACG ATGGGCGCCG TCGTATTTCA GCAAGGTTCA GCCATCGTAC CAATAAAGCT 
ATTAATGTTA CAAAAGAGCC AGACCCTCCA GTCAAAATTG AGAGCTCAGA CGAAGCTGCT 
ATTCCTGTGA GGCAGGCGCT GCAGCTACAG GACCCTGAGC AAAGAAGCAA AGAAGGAGAA 
CGACTGGGCT GCCTGGAGAC CCCGTGTCTT AATGGAGGGA CCTGTGTGAA TGGAGACACT 
TGTGACTGTC CTCTGGGATT TAAAGGGCAG CAATGCCAGT TGGCCTGCAG AAAGGAGACC 
TTTTCCTGCA CAAGACTGAT TTCTGAGACA ACATCACACC CAGTGTGGGA GGGAGGAATC 
TGCCACCACC TGTACAGAAG AGTTTACAAG GTACAGAATG ATGTGTGCAG CAGGGAGGTT 
TGTGAAGGTA CCAGCAGAAG CAGAGCAAGT GAGTGGACTC TTTTGGTTAC AATTACAGAA 
AGCAGAATCC CTTCCAAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sned1 ( XM_012970831.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826285.1 Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X2, mRNA.

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CloneID	OXa37189
Clone ID Related Accession (Same CDS sequence)	XM_012970831.3 , XM_012970831.2
Accession Version	XM_012970831.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sushi, nidogen and EGF-like domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970831.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGAAT TGGACTGTGG AGCCCCAGAG 
CAGGTAAAAT ATGCGAAGAT GATTGTGACA TCTACACTGC CGGGGGGTCG AGCAGAATAT 
CACTGTAATG AAGGCTATGT GCTCAGTACT CCAAACAACA CTAGTGTGTG CAGTGAGGAA 
GGGACATGGA GCTTGCCCCC AGAGTGTGAA GAAATAGACG AGTGTGCATC TCATCCCTGC 
CATAATGGGG CATCCTGCAA GGACCGGATT TCCCACTTTA TCTGTGAATG CCAGAAAGGG 
TACAGTGGAA AGTATTGTGA GCAAGAAACT AATGAGTGTC TGTCACAGCC ATGTAAAAAT 
GGGGGTACCT GTCAGGATTT GCCTGGGACC TTCAAATGTA TTTGTCCTGA GGGTTTCACT 
GGATCTTATT GTGAGACAGA GGTGGATGGA TGTGACTCTT CTCCGTGCCA GAATGGAGGC 
TTGTGTGAGA ACCTGGCGGG TTCTTATCTG TGCGTGTGTC CCCGCGGCTT TTTTGGCTAT 
CACTGTCAGA CAGTGAGTGA CATGTGCCTG TTGAACCCCT GCGGTAACCG TGGGGTATGT 
GTGTCCAGCG CAGATGGATT GATGAGCTGC ACCTGCAGAA CGGGGTACAC TGGAACCATG 
TGTGAGAAAG AGCTGCTGCC GCCATTGTCA CTGTCTCTGG GTGCGGTTGG AGAAAACAGT 
GTTTGGGTGT TCTGGACCCC CCCAGAAGAT GGCACTGGGC ATGTACTGGA TGGGTTTGCA 
GTCAGTTACA CTGCCCCAGA TGGCTTATCT AGAACAGACT TTGTAGAAAG AGCTCACACT 
GAACACGAGC TGCGGGGGCT TAGTCCTGGC CGCTCTTATA ACATCACAAT AGTAACAGTA 
AAGCGCAACT TGCGGCACAA TGATATCAGT CGCCCCCTCT ACCTCACAGC ACGAACACGG 
CCACCTAACG TTGACAGGCC GAGGATTAAT TCTGTCATGG ACACATCTGT CTCAGTTTCC 
TGGTCACTAA GTCCTCACAG ACACACATCT ATTGCTCGTG TTCGTCTGTT TCTCCAGCAC 
CCAACGGAGC CGCCCCAGCA GGCAGAGCAG GAGCCATCAG CAGGAGAATA TATCTTCAGG 
GATCTACGTC CTGGAGAAAA ATACAGCGTC CAAATTAGCA CCTTGAGCCG CTCTGACCCC 
AGCCACCCAG CCACAGAAAG CCTGCCCAGT GCACCTTTGA TAGCCTGGAC ACGTCCACAC 
CCACCTTCCA ACCTTACGGC AGCTCTTGTC ACAGCCAATT CTGTGCATCT AATTTGGGAG 
CAACCTCCTC ATGGGTCAGT GGATGGCTAT ATCTTCAATG TGACCAATAA TCACAGTACA 
AAGAGCCGGT ATGTTCCCAG TGGAAAGATG ACTTCATATA CAGTCCGTGA CTTGCAGGCC 
GGCCAACGCT ACCGGATAAC AGTGACTGCA CTACGCAACA CTCCCCAGGG CCCAGTGAAC 
AGTGAGCCCA AGACAGTGCG TCCACAGACA CTGCAAAGGG AGGGACCTCA GGAGCGTCGT 
TGGACCCCCC CTAGAATTCT GCGGAACAGG CCTCCTCCAC AGCCACATCA GGAGGTGCGA 
CTGCTGACAG AAAGGGGTCC TCCTCCTGCT GATACTCCAC AGCCGCACCG ATTCACAGAG 
CTGAACGATG GGCGCCGTCG TATTTCAGCA AGGTTCAGCC ATCGTACCAA TAAAGCTATT 
AATGTTACAA AAGAGCCAGA CCCTCCAGTC AAAATTGAGA GCTCAGACGA AGCTGCTATT 
CCTGTGAGGC AGGCGCTGCA GCTACAGGAC CCTGAGCAAA GAAGCAAAGA AGGAGAACGA 
CTGGGCTGCC TGGAGACCCC GTGTCTTAAT GGAGGGACCT GTGTGAATGG AGACACTTGT 
GACTGTCCTC TGGGATTTAA AGGGCAGCAA TGCCAGTTGG CCTGCAGAAA GGAGACCTTT 
TCCTGCACAA GACTGATTTC TGAGACAACA TCACACCCAG TGTGGGAGGG AGGAATCTGC 
CACCACCTGT ACAGAAGAGT TTACAAGGTA CAGAATGATG TGTGCAGCAG GGAGGTTTGT 
GAAGGTACCA GCAGAAGCAG AGCAAGTGAG TGGACTCTTT TGGTTACAAT TACAGAAAGC 
AGAATCCCTT CCAAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826285.1
CDS	1296..6296
Translation	MRLPSLFWIFISMVWRAQGMVPLEDFYPFGTKEGDTVTRRQDDGGSGLLPISKAFTFFGDKHSGLYVNNNGVVSFLREVSQFTPVAFPISGDRRVVAAFWADVDNRRGGNVFYRETEDLVIVDRATRDVRTYFPELPDFHSTWVFIATWHRVTFYGGDSTSPVNTFQIVLITDGLRSFTIFNYETIEWTTGRHASSGGDSSGRGGIAAQAGFNAGDGWRYFNIPGSRTDDIARIANTTNVGVPGRWVFRIDNNHVQVGECRNATSVCWTLRPCQNGGRCIDDCVTGNPSYTCSCLSGFTGKHCHIDMGKCYSQPCQNDGTCVDEPGSFTCLCTSDFTGTLCETEVSPCTYMTCHNGGECEDHNGIAVCVCQRGYTGDNCDTELLPCSSSPCLNGGSCENIDAGFRCQCALGFTGTHCEKEPSACFSSPCMNNGVCKDVGAGYICVCVQGFTGMYCETEIRPCSSAPCLNRGVCRENGETYSCQCARRFTGKHCETEIRPCFSNPCMNMGTCEDMSEGYSCICTEGYTGIHCETELRPCYSSPCLNGALCKDFGITYSCVCAWGFTGARCETELHPCFSSPCLNGATCRDVGFAYSCQCAQGFTGMHCETEIKPCFSSPCLNGGTCEDLAISFACLCAERYTGLHCETEIQPCFSSPCLNRGICNNEGAFYSCTCAEGFTGENCEKEVSPCLSSPCLNGATCIDMGASYSCMCPQAFTGALCDTEIPSPCTSNPCENKGVCREAEGGYLCDCPAGFSGMHCEHRVSDDCPCRNGGRCADGNSTCHCPPGHFGLLCEYEVTSLPCSMGSQCPDGGSCMEYGGSYLCVCHTDYILSNHSIPSPCDSDPCLNGGTCESLDDAYTCACPRGFNGRLCEKVKPALCSLSPCRNGGTCKESREEYYCVCPYPFTGKHCETGMRGPCSSGPCNNGGTCFHYLGKYKCDCPPNFSGRHCEKELDCGAPEQVKYAKMIVTSTLPGGRAEYHCNEGYVLSTPNNTSVCSEEGTWSLPPECEEIDECASHPCHNGASCKDRISHFICECQKGYSGKYCEQETNECLSQPCKNGGTCQDLPGTFKCICPEGFTGSYCETEVDGCDSSPCQNGGLCENLAGSYLCVCPRGFFGYHCQTVSDMCLLNPCGNRGVCVSSADGLMSCTCRTGYTGTMCEKELLPPLSLSLGAVGENSVWVFWTPPEDGTGHVLDGFAVSYTAPDGLSRTDFVERAHTEHELRGLSPGRSYNITIVTVKRNLRHNDISRPLYLTARTRPPNVDRPRINSVMDTSVSVSWSLSPHRHTSIARVRLFLQHPTEPPQQAEQEPSAGEYIFRDLRPGEKYSVQISTLSRSDPSHPATESLPSAPLIAWTRPHPPSNLTAALVTANSVHLIWEQPPHGSVDGYIFNVTNNHSTKSRYVPSGKMTSYTVRDLQAGQRYRITVTALRNTPQGPVNSEPKTVRPQTLQREGPQERRWTPPRILRNRPPPQPHQEVRLLTERGPPPADTPQPHRFTELNDGRRRISARFSHRTNKAINVTKEPDPPVKIESSDEAAIPVRQALQLQDPEQRSKEGERLGCLETPCLNGGTCVNGDTCDCPLGFKGQQCQLACRKETFSCTRLISETTSHPVWEGGICHHLYRRVYKVQNDVCSREVCEGTSRSRASEWTLLVTITESRIPSKP

Target ORF information:

RefSeq Version	XM_012970831.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970831.3

ORF Insert Sequence:

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGAAT TGGACTGTGG AGCCCCAGAG 
CAGGTAAAAT ATGCGAAGAT GATTGTGACA TCTACACTGC CGGGGGGTCG AGCAGAATAT 
CACTGTAATG AAGGCTATGT GCTCAGTACT CCAAACAACA CTAGTGTGTG CAGTGAGGAA 
GGGACATGGA GCTTGCCCCC AGAGTGTGAA GAAATAGACG AGTGTGCATC TCATCCCTGC 
CATAATGGGG CATCCTGCAA GGACCGGATT TCCCACTTTA TCTGTGAATG CCAGAAAGGG 
TACAGTGGAA AGTATTGTGA GCAAGAAACT AATGAGTGTC TGTCACAGCC ATGTAAAAAT 
GGGGGTACCT GTCAGGATTT GCCTGGGACC TTCAAATGTA TTTGTCCTGA GGGTTTCACT 
GGATCTTATT GTGAGACAGA GGTGGATGGA TGTGACTCTT CTCCGTGCCA GAATGGAGGC 
TTGTGTGAGA ACCTGGCGGG TTCTTATCTG TGCGTGTGTC CCCGCGGCTT TTTTGGCTAT 
CACTGTCAGA CAGTGAGTGA CATGTGCCTG TTGAACCCCT GCGGTAACCG TGGGGTATGT 
GTGTCCAGCG CAGATGGATT GATGAGCTGC ACCTGCAGAA CGGGGTACAC TGGAACCATG 
TGTGAGAAAG AGCTGCTGCC GCCATTGTCA CTGTCTCTGG GTGCGGTTGG AGAAAACAGT 
GTTTGGGTGT TCTGGACCCC CCCAGAAGAT GGCACTGGGC ATGTACTGGA TGGGTTTGCA 
GTCAGTTACA CTGCCCCAGA TGGCTTATCT AGAACAGACT TTGTAGAAAG AGCTCACACT 
GAACACGAGC TGCGGGGGCT TAGTCCTGGC CGCTCTTATA ACATCACAAT AGTAACAGTA 
AAGCGCAACT TGCGGCACAA TGATATCAGT CGCCCCCTCT ACCTCACAGC ACGAACACGG 
CCACCTAACG TTGACAGGCC GAGGATTAAT TCTGTCATGG ACACATCTGT CTCAGTTTCC 
TGGTCACTAA GTCCTCACAG ACACACATCT ATTGCTCGTG TTCGTCTGTT TCTCCAGCAC 
CCAACGGAGC CGCCCCAGCA GGCAGAGCAG GAGCCATCAG CAGGAGAATA TATCTTCAGG 
GATCTACGTC CTGGAGAAAA ATACAGCGTC CAAATTAGCA CCTTGAGCCG CTCTGACCCC 
AGCCACCCAG CCACAGAAAG CCTGCCCAGT GCACCTTTGA TAGCCTGGAC ACGTCCACAC 
CCACCTTCCA ACCTTACGGC AGCTCTTGTC ACAGCCAATT CTGTGCATCT AATTTGGGAG 
CAACCTCCTC ATGGGTCAGT GGATGGCTAT ATCTTCAATG TGACCAATAA TCACAGTACA 
AAGAGCCGGT ATGTTCCCAG TGGAAAGATG ACTTCATATA CAGTCCGTGA CTTGCAGGCC 
GGCCAACGCT ACCGGATAAC AGTGACTGCA CTACGCAACA CTCCCCAGGG CCCAGTGAAC 
AGTGAGCCCA AGACAGTGCG TCCACAGACA CTGCAAAGGG AGGGACCTCA GGAGCGTCGT 
TGGACCCCCC CTAGAATTCT GCGGAACAGG CCTCCTCCAC AGCCACATCA GGAGGTGCGA 
CTGCTGACAG AAAGGGGTCC TCCTCCTGCT GATACTCCAC AGCCGCACCG ATTCACAGAG 
CTGAACGATG GGCGCCGTCG TATTTCAGCA AGGTTCAGCC ATCGTACCAA TAAAGCTATT 
AATGTTACAA AAGAGCCAGA CCCTCCAGTC AAAATTGAGA GCTCAGACGA AGCTGCTATT 
CCTGTGAGGC AGGCGCTGCA GCTACAGGAC CCTGAGCAAA GAAGCAAAGA AGGAGAACGA 
CTGGGCTGCC TGGAGACCCC GTGTCTTAAT GGAGGGACCT GTGTGAATGG AGACACTTGT 
GACTGTCCTC TGGGATTTAA AGGGCAGCAA TGCCAGTTGG CCTGCAGAAA GGAGACCTTT 
TCCTGCACAA GACTGATTTC TGAGACAACA TCACACCCAG TGTGGGAGGG AGGAATCTGC 
CACCACCTGT ACAGAAGAGT TTACAAGGTA CAGAATGATG TGTGCAGCAG GGAGGTTTGT 
GAAGGTACCA GCAGAAGCAG AGCAAGTGAG TGGACTCTTT TGGTTACAAT TACAGAAAGC 
AGAATCCCTT CCAAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sned1 ( XM_031902549.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758409.1 Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X4, mRNA.

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CloneID	OXa37190
Clone ID Related Accession (Same CDS sequence)	XM_031902549.1
Accession Version	XM_031902549.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sushi, nidogen and EGF-like domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AACTCTTGCC ATGCTCTTCC TCTCCTTGTT TGAATGGTGG GTCCTGCGAG 
AATATTGATG CTGGGTTTCG CTGTCAGTGC GCTTTGGGAT TTACTGGAAC ACACTGTGAA 
AAAGAACCCA GCGCATGCTT TTCGTCTCCC TGTATGAATA ACGGAGTCTG CAAGGATGTG 
GGTGCTGGAT ACATCTGTGT GTGTGTTCAG GGATTCACTG GAATGTATTG TGAAACAGAG 
ATCAGGCCAT GTTCTTCAGC TCCATGTTTA AACAGAGGTG TGTGCAGAGA GAATGGCGAG 
ACTTACAGCT GTCAGTGTGC ACGCCGATTC ACCGGAAAGC ATTGCGAGAC AGAGATCAGA 
CCTTGTTTTT CTAATCCCTG TATGAACATG GGGACATGTG AGGACATGAG TGAAGGTTAT 
AGCTGTATCT GCACAGAAGG TTACACTGGA ATCCACTGTG AGACAGAGTT AAGACCCTGC 
TATTCATCCC CTTGCCTTAA TGGGGCATTG TGCAAGGACT TTGGCATTAC TTACAGCTGT 
GTGTGTGCAT GGGGGTTCAC TGGTGCACGA TGTGAGACAG AGCTGCACCC ATGCTTTTCT 
TCTCCTTGCC TGAATGGAGC AACATGCAGA GATGTCGGTT TTGCTTACAG CTGTCAGTGT 
GCACAGGGAT TCACAGGGAT GCACTGTGAG ACAGAGATCA AGCCATGTTT TTCATCCCCT 
TGTCTGAACG GAGGGACCTG TGAGGACCTG GCAATCAGCT TTGCCTGTTT ATGTGCAGAG 
AGATATACTG GCTTGCACTG CGAGACAGAG ATACAGCCTT GCTTTTCCTC TCCATGCCTG 
AATAGAGGAA TCTGTAATAA TGAAGGTGCA TTTTACAGCT GTACATGTGC AGAGGGCTTT 
ACAGGAGAAA ACTGTGAAAA AGAGGTGAGC CCTTGCCTTT CTTCTCCCTG TCTGAATGGA 
GCAACCTGCA TTGACATGGG TGCAAGTTAC AGCTGCATGT GCCCACAGGC CTTCACTGGG 
GCGCTCTGTG ACACGGAGAT ACCAAGCCCA TGTACATCAA ATCCTTGTGA GAACAAAGGT 
GTGTGCAGGG AGGCAGAAGG AGGCTACTTG TGTGACTGCC CCGCAGGTTT CAGTGGCATG 
CACTGCGAGC ACCGTGTGTC CGATGACTGC CCCTGCAGGA ATGGAGGGCG ATGTGCAGAT 
GGAAACAGCA CTTGTCACTG CCCGCCAGGA CACTTTGGGC TTCTCTGTGA ATATGAGGTC 
ACCTCGCTAC CGTGCAGCAT GGGTTCACAG TGTCCAGATG GCGGATCCTG CATGGAATAT 
GGTGGAAGTT ACTTGTGTGT GTGTCACACA GATTACATCC TTAGTAACCA CTCAATACCC 
TCACCCTGTG ACTCGGATCC CTGCCTGAAT GGTGGAACAT GTGAATCTCT AGATGATGCA 
TATACATGTG CCTGTCCCCG TGGCTTCAAT GGAAGACTTT GTGAGAAAGT AAAGCCAGCT 
CTGTGCAGCC TATCACCTTG CCGCAATGGG GGCACCTGCA AGGAGTCACG AGAAGAATAT 
TATTGCGTTT GTCCCTATCC ATTCACTGGA AAACACTGTG AGACTGGTAT GAGGGGCCCT 
TGTTCGTCAG GTCCGTGTAA TAATGGTGGT ACATGTTTCC ATTATCTTGG GAAATACAAG 
TGCGACTGCC CACCAAACTT CTCTGGGCGA CACTGTGAGA AAGCAGAATT GGACTGTGGA 
GCCCCAGAGC AGGTAAAATA TGCGAAGATG ATTGTGACAT CTACACTGCC GGGGGGTCGA 
GCAGAATATC ACTGTAATGA AGGCTATGTG CTCAGTACTC CAAACAACAC TAGTGTGTGC 
AGTGAGGAAG GGACATGGAG CTTGCCCCCA GAGTGTGAAG AAATAGACGA GTGTGCATCT 
CATCCCTGCC ATAATGGGGC ATCCTGCAAG GACCGGATTT CCCACTTTAT CTGTGAATGC 
CAGAAAGGGT ACAGTGGAAA GTATTGTGAG CAAGAAACTA ATGAGTGTCT GTCACAGCCA 
TGTAAAAATG GGGGTACCTG TCAGGATTTG CCTGGGACCT TCAAATGTAT TTGTCCTGAG 
GGTTTCACTG GATCTTATTG TGAGACAGAG GTGGATGGAT GTGACTCTTC TCCGTGCCAG 
AATGGAGGCT TGTGTGAGAA CCTGGCGGGT TCTTATCTGT GCGTGTGTCC CCGCGGCTTT 
TTTGGCTATC ACTGTCAGAC AGTGAGTGAC ATGTGCCTGT TGAACCCCTG CGGTAACCGT 
GGGGTATGTG TGTCCAGCGC AGATGGATTG ATGAGCTGCA CCTGCAGAAC GGGGTACACT 
GGAACCATGT GTGAGAAAGA GCTGCTGCCG CCATTGTCAC TGTCTCTGGG TGCGGTTGGA 
GAAAACAGTG TTTGGGTGTT CTGGACCCCC CCAGAAGATG GCACTGGGCA TGTACTGGAT 
GGGTTTGCAG TCAGTTACAC TGCCCCAGAT GGCTTATCTA GAACAGACTT TGTAGAAAGA 
GCTCACACTG AACACGAGCT GCGGGGGCTT AGTCCTGGCC GCTCTTATAA CATCACAATA 
GTAACAGTAA AGCGCAACTT GCGGCACAAT GATATCAGTC GCCCCCTCTA CCTCACAGCA 
CGAACACGGC CACCTAACGT TGACAGGCCG AGGATTAATT CTGTCATGGA CACATCTGTC 
TCAGTTTCCT GGTCACTAAG TCCTCACAGA CACACATCTA TTGCTCGTGT TCGTCTGTTT 
CTCCAGCACC CAACGGAGCC GCCCCAGCAG GCAGAGCAGG AGCCATCAGC AGGAGAATAT 
ATCTTCAGGG ATCTACGTCC TGGAGAAAAA TACAGCGTCC AAATTAGCAC CTTGAGCCGC 
TCTGACCCCA GCCACCCAGC CACAGAAAGC CTGCCCAGTG CACCTTTGAT AGCCTGGACA 
CGTCCACACC CACCTTCCAA CCTTACGGCA GCTCTTGTCA CAGCCAATTC TGTGCATCTA 
ATTTGGGAGC AACCTCCTCA TGGGTCAGTG GATGGCTATA TCTTCAATGT GACCAATAAT 
CACAGTACAA AGAGCCGGTA TGTTCCCAGT GGAAAGATGA CTTCATATAC AGTCCGTGAC 
TTGCAGGCCG GCCAACGCTA CCGGATAACA GTGACTGCAC TACGCAACAC TCCCCAGGGC 
CCAGTGAACA GTGAGCCCAA GACAGTGCGT CCACAGACAC TGCAAAGGGA GGGACCTCAG 
GAGCGTCGTT GGACCCCCCC TAGAATTCTG CGGAACAGGC CTCCTCCACA GCCACATCAG 
GAGGTGCGAC TGCTGACAGA AAGGGGTCCT CCTCCTGCTG ATACTCCACA GCCGCACCGA 
TTCACAGAGC TGAACGATGG GCGCCGTCGT ATTTCAGCAA GGTTCAGCCA TCGTACCAAT 
AAAGCTATTA ATGTTACAAA AGAGCCAGAC CCTCCAGTCA AAATTGAGAG CTCAGACGAA 
GCTGCTATTC CTGTGAGGCA GGCGCTGCAG CTACAGGACC CTGAGCAAAG AAGCAAAGAA 
GGAGAACGAC TGGGCTGCCT GGAGACCCCG TGTCTTAATG GAGGGACCTG TGTGAATGGA 
GACACTTGTG ACTGTCCTCT GGGATTTAAA GGGCAGCAAT GCCAGTTGGC CTGCAGAAAG 
GAGACCTTTT CCTGCACAAG ACTGATTTCT GAGACAACAT CACACCCAGT GTGGGAGGGA 
GGAATCTGCC ACCACCTGTA CAGAAGAGTT TACAAGGTAC AGAATGATGT GTGCAGCAGG 
GAGGTTTGTG AAGGTACCAG CAGAAGCAGA GCAAGTGAGT GGACTCTTTT GGTTACAATT 
ACAGAAAGCA GAATCCCTTC CAAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758409.1
CDS	1295..6184
Translation	MRLPSLFWIFISMVWRAQGMVPLEDFYPFGTKEGDTVTRRQDDGGSGLLPISKAFTFFGDKHSGLYVNNNGVVSFLREVSQFTPVAFPISGDRRVVAAFWADVDNRRGGNVFYRETEDLVIVDRATRDVRTYFPELPDFHSTWVFIATWHRVTFYGGDSTSPVNTFQIVLITDGLRSFTIFNYETIEWTTGRHASSGGDSSGRGGIAAQAGFNAGDGWRYFNIPGSRTDDIARIANTTNVGVPGRWVFRIDNNHVQVGECRNATSVCWTLRPCQNGGRCIDDCVTGNPSYTCSCLSGFTGKHCHIDMGKCYSQPCQNDGTCVDEPGSFTCLCTSDFTGTLCETELLPCSSSPCLNGGSCENIDAGFRCQCALGFTGTHCEKEPSACFSSPCMNNGVCKDVGAGYICVCVQGFTGMYCETEIRPCSSAPCLNRGVCRENGETYSCQCARRFTGKHCETEIRPCFSNPCMNMGTCEDMSEGYSCICTEGYTGIHCETELRPCYSSPCLNGALCKDFGITYSCVCAWGFTGARCETELHPCFSSPCLNGATCRDVGFAYSCQCAQGFTGMHCETEIKPCFSSPCLNGGTCEDLAISFACLCAERYTGLHCETEIQPCFSSPCLNRGICNNEGAFYSCTCAEGFTGENCEKEVSPCLSSPCLNGATCIDMGASYSCMCPQAFTGALCDTEIPSPCTSNPCENKGVCREAEGGYLCDCPAGFSGMHCEHRVSDDCPCRNGGRCADGNSTCHCPPGHFGLLCEYEVTSLPCSMGSQCPDGGSCMEYGGSYLCVCHTDYILSNHSIPSPCDSDPCLNGGTCESLDDAYTCACPRGFNGRLCEKVKPALCSLSPCRNGGTCKESREEYYCVCPYPFTGKHCETGMRGPCSSGPCNNGGTCFHYLGKYKCDCPPNFSGRHCEKAELDCGAPEQVKYAKMIVTSTLPGGRAEYHCNEGYVLSTPNNTSVCSEEGTWSLPPECEEIDECASHPCHNGASCKDRISHFICECQKGYSGKYCEQETNECLSQPCKNGGTCQDLPGTFKCICPEGFTGSYCETEVDGCDSSPCQNGGLCENLAGSYLCVCPRGFFGYHCQTVSDMCLLNPCGNRGVCVSSADGLMSCTCRTGYTGTMCEKELLPPLSLSLGAVGENSVWVFWTPPEDGTGHVLDGFAVSYTAPDGLSRTDFVERAHTEHELRGLSPGRSYNITIVTVKRNLRHNDISRPLYLTARTRPPNVDRPRINSVMDTSVSVSWSLSPHRHTSIARVRLFLQHPTEPPQQAEQEPSAGEYIFRDLRPGEKYSVQISTLSRSDPSHPATESLPSAPLIAWTRPHPPSNLTAALVTANSVHLIWEQPPHGSVDGYIFNVTNNHSTKSRYVPSGKMTSYTVRDLQAGQRYRITVTALRNTPQGPVNSEPKTVRPQTLQREGPQERRWTPPRILRNRPPPQPHQEVRLLTERGPPPADTPQPHRFTELNDGRRRISARFSHRTNKAINVTKEPDPPVKIESSDEAAIPVRQALQLQDPEQRSKEGERLGCLETPCLNGGTCVNGDTCDCPLGFKGQQCQLACRKETFSCTRLISETTSHPVWEGGICHHLYRRVYKVQNDVCSREVCEGTSRSRASEWTLLVTITESRIPSKP

Target ORF information:

RefSeq Version	XM_031902549.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902549.1

ORF Insert Sequence:

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AACTCTTGCC ATGCTCTTCC TCTCCTTGTT TGAATGGTGG GTCCTGCGAG 
AATATTGATG CTGGGTTTCG CTGTCAGTGC GCTTTGGGAT TTACTGGAAC ACACTGTGAA 
AAAGAACCCA GCGCATGCTT TTCGTCTCCC TGTATGAATA ACGGAGTCTG CAAGGATGTG 
GGTGCTGGAT ACATCTGTGT GTGTGTTCAG GGATTCACTG GAATGTATTG TGAAACAGAG 
ATCAGGCCAT GTTCTTCAGC TCCATGTTTA AACAGAGGTG TGTGCAGAGA GAATGGCGAG 
ACTTACAGCT GTCAGTGTGC ACGCCGATTC ACCGGAAAGC ATTGCGAGAC AGAGATCAGA 
CCTTGTTTTT CTAATCCCTG TATGAACATG GGGACATGTG AGGACATGAG TGAAGGTTAT 
AGCTGTATCT GCACAGAAGG TTACACTGGA ATCCACTGTG AGACAGAGTT AAGACCCTGC 
TATTCATCCC CTTGCCTTAA TGGGGCATTG TGCAAGGACT TTGGCATTAC TTACAGCTGT 
GTGTGTGCAT GGGGGTTCAC TGGTGCACGA TGTGAGACAG AGCTGCACCC ATGCTTTTCT 
TCTCCTTGCC TGAATGGAGC AACATGCAGA GATGTCGGTT TTGCTTACAG CTGTCAGTGT 
GCACAGGGAT TCACAGGGAT GCACTGTGAG ACAGAGATCA AGCCATGTTT TTCATCCCCT 
TGTCTGAACG GAGGGACCTG TGAGGACCTG GCAATCAGCT TTGCCTGTTT ATGTGCAGAG 
AGATATACTG GCTTGCACTG CGAGACAGAG ATACAGCCTT GCTTTTCCTC TCCATGCCTG 
AATAGAGGAA TCTGTAATAA TGAAGGTGCA TTTTACAGCT GTACATGTGC AGAGGGCTTT 
ACAGGAGAAA ACTGTGAAAA AGAGGTGAGC CCTTGCCTTT CTTCTCCCTG TCTGAATGGA 
GCAACCTGCA TTGACATGGG TGCAAGTTAC AGCTGCATGT GCCCACAGGC CTTCACTGGG 
GCGCTCTGTG ACACGGAGAT ACCAAGCCCA TGTACATCAA ATCCTTGTGA GAACAAAGGT 
GTGTGCAGGG AGGCAGAAGG AGGCTACTTG TGTGACTGCC CCGCAGGTTT CAGTGGCATG 
CACTGCGAGC ACCGTGTGTC CGATGACTGC CCCTGCAGGA ATGGAGGGCG ATGTGCAGAT 
GGAAACAGCA CTTGTCACTG CCCGCCAGGA CACTTTGGGC TTCTCTGTGA ATATGAGGTC 
ACCTCGCTAC CGTGCAGCAT GGGTTCACAG TGTCCAGATG GCGGATCCTG CATGGAATAT 
GGTGGAAGTT ACTTGTGTGT GTGTCACACA GATTACATCC TTAGTAACCA CTCAATACCC 
TCACCCTGTG ACTCGGATCC CTGCCTGAAT GGTGGAACAT GTGAATCTCT AGATGATGCA 
TATACATGTG CCTGTCCCCG TGGCTTCAAT GGAAGACTTT GTGAGAAAGT AAAGCCAGCT 
CTGTGCAGCC TATCACCTTG CCGCAATGGG GGCACCTGCA AGGAGTCACG AGAAGAATAT 
TATTGCGTTT GTCCCTATCC ATTCACTGGA AAACACTGTG AGACTGGTAT GAGGGGCCCT 
TGTTCGTCAG GTCCGTGTAA TAATGGTGGT ACATGTTTCC ATTATCTTGG GAAATACAAG 
TGCGACTGCC CACCAAACTT CTCTGGGCGA CACTGTGAGA AAGCAGAATT GGACTGTGGA 
GCCCCAGAGC AGGTAAAATA TGCGAAGATG ATTGTGACAT CTACACTGCC GGGGGGTCGA 
GCAGAATATC ACTGTAATGA AGGCTATGTG CTCAGTACTC CAAACAACAC TAGTGTGTGC 
AGTGAGGAAG GGACATGGAG CTTGCCCCCA GAGTGTGAAG AAATAGACGA GTGTGCATCT 
CATCCCTGCC ATAATGGGGC ATCCTGCAAG GACCGGATTT CCCACTTTAT CTGTGAATGC 
CAGAAAGGGT ACAGTGGAAA GTATTGTGAG CAAGAAACTA ATGAGTGTCT GTCACAGCCA 
TGTAAAAATG GGGGTACCTG TCAGGATTTG CCTGGGACCT TCAAATGTAT TTGTCCTGAG 
GGTTTCACTG GATCTTATTG TGAGACAGAG GTGGATGGAT GTGACTCTTC TCCGTGCCAG 
AATGGAGGCT TGTGTGAGAA CCTGGCGGGT TCTTATCTGT GCGTGTGTCC CCGCGGCTTT 
TTTGGCTATC ACTGTCAGAC AGTGAGTGAC ATGTGCCTGT TGAACCCCTG CGGTAACCGT 
GGGGTATGTG TGTCCAGCGC AGATGGATTG ATGAGCTGCA CCTGCAGAAC GGGGTACACT 
GGAACCATGT GTGAGAAAGA GCTGCTGCCG CCATTGTCAC TGTCTCTGGG TGCGGTTGGA 
GAAAACAGTG TTTGGGTGTT CTGGACCCCC CCAGAAGATG GCACTGGGCA TGTACTGGAT 
GGGTTTGCAG TCAGTTACAC TGCCCCAGAT GGCTTATCTA GAACAGACTT TGTAGAAAGA 
GCTCACACTG AACACGAGCT GCGGGGGCTT AGTCCTGGCC GCTCTTATAA CATCACAATA 
GTAACAGTAA AGCGCAACTT GCGGCACAAT GATATCAGTC GCCCCCTCTA CCTCACAGCA 
CGAACACGGC CACCTAACGT TGACAGGCCG AGGATTAATT CTGTCATGGA CACATCTGTC 
TCAGTTTCCT GGTCACTAAG TCCTCACAGA CACACATCTA TTGCTCGTGT TCGTCTGTTT 
CTCCAGCACC CAACGGAGCC GCCCCAGCAG GCAGAGCAGG AGCCATCAGC AGGAGAATAT 
ATCTTCAGGG ATCTACGTCC TGGAGAAAAA TACAGCGTCC AAATTAGCAC CTTGAGCCGC 
TCTGACCCCA GCCACCCAGC CACAGAAAGC CTGCCCAGTG CACCTTTGAT AGCCTGGACA 
CGTCCACACC CACCTTCCAA CCTTACGGCA GCTCTTGTCA CAGCCAATTC TGTGCATCTA 
ATTTGGGAGC AACCTCCTCA TGGGTCAGTG GATGGCTATA TCTTCAATGT GACCAATAAT 
CACAGTACAA AGAGCCGGTA TGTTCCCAGT GGAAAGATGA CTTCATATAC AGTCCGTGAC 
TTGCAGGCCG GCCAACGCTA CCGGATAACA GTGACTGCAC TACGCAACAC TCCCCAGGGC 
CCAGTGAACA GTGAGCCCAA GACAGTGCGT CCACAGACAC TGCAAAGGGA GGGACCTCAG 
GAGCGTCGTT GGACCCCCCC TAGAATTCTG CGGAACAGGC CTCCTCCACA GCCACATCAG 
GAGGTGCGAC TGCTGACAGA AAGGGGTCCT CCTCCTGCTG ATACTCCACA GCCGCACCGA 
TTCACAGAGC TGAACGATGG GCGCCGTCGT ATTTCAGCAA GGTTCAGCCA TCGTACCAAT 
AAAGCTATTA ATGTTACAAA AGAGCCAGAC CCTCCAGTCA AAATTGAGAG CTCAGACGAA 
GCTGCTATTC CTGTGAGGCA GGCGCTGCAG CTACAGGACC CTGAGCAAAG AAGCAAAGAA 
GGAGAACGAC TGGGCTGCCT GGAGACCCCG TGTCTTAATG GAGGGACCTG TGTGAATGGA 
GACACTTGTG ACTGTCCTCT GGGATTTAAA GGGCAGCAAT GCCAGTTGGC CTGCAGAAAG 
GAGACCTTTT CCTGCACAAG ACTGATTTCT GAGACAACAT CACACCCAGT GTGGGAGGGA 
GGAATCTGCC ACCACCTGTA CAGAAGAGTT TACAAGGTAC AGAATGATGT GTGCAGCAGG 
GAGGTTTGTG AAGGTACCAG CAGAAGCAGA GCAAGTGAGT GGACTCTTTT GGTTACAATT 
ACAGAAAGCA GAATCCCTTC CAAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sned1 ( XM_012970833.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826287.1 Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X5, mRNA.

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CloneID	OXa37191
Clone ID Related Accession (Same CDS sequence)	XM_012970833.3 , XM_012970833.2
Accession Version	XM_012970833.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sushi, nidogen and EGF-like domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970833.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA 
GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA 
TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG 
GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAACTA ATGAGTGTCT GTCACAGCCA 
TGTAAAAATG GGGGTACCTG TCAGGATTTG CCTGGGACCT TCAAATGTAT TTGTCCTGAG 
GGTTTCACTG GATCTTATTG TGAGACAGAG GTGGATGGAT GTGACTCTTC TCCGTGCCAG 
AATGGAGGCT TGTGTGAGAA CCTGGCGGGT TCTTATCTGT GCGTGTGTCC CCGCGGCTTT 
TTTGGCTATC ACTGTCAGAC AGTGAGTGAC ATGTGCCTGT TGAACCCCTG CGGTAACCGT 
GGGGTATGTG TGTCCAGCGC AGATGGATTG ATGAGCTGCA CCTGCAGAAC GGGGTACACT 
GGAACCATGT GTGAGAAAGA GCTGCTGCCG CCATTGTCAC TGTCTCTGGG TGCGGTTGGA 
GAAAACAGTG TTTGGGTGTT CTGGACCCCC CCAGAAGATG GCACTGGGCA TGTACTGGAT 
GGGTTTGCAG TCAGTTACAC TGCCCCAGAT GGCTTATCTA GAACAGACTT TGTAGAAAGA 
GCTCACACTG AACACGAGCT GCGGGGGCTT AGTCCTGGCC GCTCTTATAA CATCACAATA 
GTAACAGTAA AGCGCAACTT GCGGCACAAT GATATCAGTC GCCCCCTCTA CCTCACAGCA 
CGAACACGGC CACCTAACGT TGACAGGCCG AGGATTAATT CTGTCATGGA CACATCTGTC 
TCAGTTTCCT GGTCACTAAG TCCTCACAGA CACACATCTA TTGCTCGTGT TCGTCTGTTT 
CTCCAGCACC CAACGGAGCC GCCCCAGCAG GCAGAGCAGG AGCCATCAGC AGGAGAATAT 
ATCTTCAGGG ATCTACGTCC TGGAGAAAAA TACAGCGTCC AAATTAGCAC CTTGAGCCGC 
TCTGACCCCA GCCACCCAGC CACAGAAAGC CTGCCCAGTG CACCTTTGAT AGCCTGGACA 
CGTCCACACC CACCTTCCAA CCTTACGGCA GCTCTTGTCA CAGCCAATTC TGTGCATCTA 
ATTTGGGAGC AACCTCCTCA TGGGTCAGTG GATGGCTATA TCTTCAATGT GACCAATAAT 
CACAGTACAA AGAGCCGGTA TGTTCCCAGT GGAAAGATGA CTTCATATAC AGTCCGTGAC 
TTGCAGGCCG GCCAACGCTA CCGGATAACA GTGACTGCAC TACGCAACAC TCCCCAGGGC 
CCAGTGAACA GTGAGCCCAA GACAGTGCGT CCACAGACAC TGCAAAGGGA GGGACCTCAG 
GAGCGTCGTT GGACCCCCCC TAGAATTCTG CGGAACAGGC CTCCTCCACA GCCACATCAG 
GAGGTGCGAC TGCTGACAGA AAGGGGTCCT CCTCCTGCTG ATACTCCACA GCCGCACCGA 
TTCACAGAGC TGAACGATGG GCGCCGTCGT ATTTCAGCAA GGTTCAGCCA TCGTACCAAT 
AAAGCTATTA ATGTTACAAA AGAGCCAGAC CCTCCAGTCA AAATTGAGAG CTCAGACGAA 
GCTGCTATTC CTGTGAGGCA GGCGCTGCAG CTACAGGACC CTGAGCAAAG AAGCAAAGAA 
GGAGAACGAC TGGGCTGCCT GGAGACCCCG TGTCTTAATG GAGGGACCTG TGTGAATGGA 
GACACTTGTG ACTGTCCTCT GGGATTTAAA GGGCAGCAAT GCCAGTTGGC CTGCAGAAAG 
GAGACCTTTT CCTGCACAAG ACTGATTTCT GAGACAACAT CACACCCAGT GTGGGAGGGA 
GGAATCTGCC ACCACCTGTA CAGAAGAGTT TACAAGGTAC AGAATGATGT GTGCAGCAGG 
GAGGTTTGTG AAGGTACCAG CAGAAGCAGA GCAAGTGAGT GGACTCTTTT GGTTACAATT 
ACAGAAAGCA GAATCCCTTC CAAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826287.1
CDS	1299..6188
Translation	MRLPSLFWIFISMVWRAQGMVPLEDFYPFGTKEGDTVTRRQDDGGSGLLPISKAFTFFGDKHSGLYVNNNGVVSFLREVSQFTPVAFPISGDRRVVAAFWADVDNRRGGNVFYRETEDLVIVDRATRDVRTYFPELPDFHSTWVFIATWHRVTFYGGDSTSPVNTFQIVLITDGLRSFTIFNYETIEWTTGRHASSGGDSSGRGGIAAQAGFNAGDGWRYFNIPGSRTDDIARIANTTNVGVPGRWVFRIDNNHVQVGECRNATSVCWTLRPCQNGGRCIDDCVTGNPSYTCSCLSGFTGKHCHIDMGKCYSQPCQNDGTCVDEPGSFTCLCTSDFTGTLCETEVSPCTYMTCHNGGECEDHNGIAVCVCQRGYTGDNCDTELLPCSSSPCLNGGSCENIDAGFRCQCALGFTGTHCEKEPSACFSSPCMNNGVCKDVGAGYICVCVQGFTGMYCETEIRPCSSAPCLNRGVCRENGETYSCQCARRFTGKHCETEIRPCFSNPCMNMGTCEDMSEGYSCICTEGYTGIHCETELRPCYSSPCLNGALCKDFGITYSCVCAWGFTGARCETELHPCFSSPCLNGATCRDVGFAYSCQCAQGFTGMHCETEIKPCFSSPCLNGGTCEDLAISFACLCAERYTGLHCETEIQPCFSSPCLNRGICNNEGAFYSCTCAEGFTGENCEKEVSPCLSSPCLNGATCIDMGASYSCMCPQAFTGALCDTEIPSPCTSNPCENKGVCREAEGGYLCDCPAGFSGMHCEHRVSDDCPCRNGGRCADGNSTCHCPPGHFGLLCEYEVTSLPCSMGSQCPDGGSCMEYGGSYLCVCHTDYILSNHSIPSPCDSDPCLNGGTCESLDDAYTCACPRGFNGRLCEKVKPALCSLSPCRNGGTCKESREEYYCVCPYPFTGKHCETGMRGPCSSGPCNNGGTCFHYLGKYKCDCPPNFSGRHCEKAELDCGAPEQVKYAKMIVTSTLPGGRAEYHCNEGYVLSTPNNTSVCSEEGTWSLPPECEETNECLSQPCKNGGTCQDLPGTFKCICPEGFTGSYCETEVDGCDSSPCQNGGLCENLAGSYLCVCPRGFFGYHCQTVSDMCLLNPCGNRGVCVSSADGLMSCTCRTGYTGTMCEKELLPPLSLSLGAVGENSVWVFWTPPEDGTGHVLDGFAVSYTAPDGLSRTDFVERAHTEHELRGLSPGRSYNITIVTVKRNLRHNDISRPLYLTARTRPPNVDRPRINSVMDTSVSVSWSLSPHRHTSIARVRLFLQHPTEPPQQAEQEPSAGEYIFRDLRPGEKYSVQISTLSRSDPSHPATESLPSAPLIAWTRPHPPSNLTAALVTANSVHLIWEQPPHGSVDGYIFNVTNNHSTKSRYVPSGKMTSYTVRDLQAGQRYRITVTALRNTPQGPVNSEPKTVRPQTLQREGPQERRWTPPRILRNRPPPQPHQEVRLLTERGPPPADTPQPHRFTELNDGRRRISARFSHRTNKAINVTKEPDPPVKIESSDEAAIPVRQALQLQDPEQRSKEGERLGCLETPCLNGGTCVNGDTCDCPLGFKGQQCQLACRKETFSCTRLISETTSHPVWEGGICHHLYRRVYKVQNDVCSREVCEGTSRSRASEWTLLVTITESRIPSKP

Target ORF information:

RefSeq Version	XM_012970833.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970833.3

ORF Insert Sequence:

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA 
GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA 
TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG 
GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAACTA ATGAGTGTCT GTCACAGCCA 
TGTAAAAATG GGGGTACCTG TCAGGATTTG CCTGGGACCT TCAAATGTAT TTGTCCTGAG 
GGTTTCACTG GATCTTATTG TGAGACAGAG GTGGATGGAT GTGACTCTTC TCCGTGCCAG 
AATGGAGGCT TGTGTGAGAA CCTGGCGGGT TCTTATCTGT GCGTGTGTCC CCGCGGCTTT 
TTTGGCTATC ACTGTCAGAC AGTGAGTGAC ATGTGCCTGT TGAACCCCTG CGGTAACCGT 
GGGGTATGTG TGTCCAGCGC AGATGGATTG ATGAGCTGCA CCTGCAGAAC GGGGTACACT 
GGAACCATGT GTGAGAAAGA GCTGCTGCCG CCATTGTCAC TGTCTCTGGG TGCGGTTGGA 
GAAAACAGTG TTTGGGTGTT CTGGACCCCC CCAGAAGATG GCACTGGGCA TGTACTGGAT 
GGGTTTGCAG TCAGTTACAC TGCCCCAGAT GGCTTATCTA GAACAGACTT TGTAGAAAGA 
GCTCACACTG AACACGAGCT GCGGGGGCTT AGTCCTGGCC GCTCTTATAA CATCACAATA 
GTAACAGTAA AGCGCAACTT GCGGCACAAT GATATCAGTC GCCCCCTCTA CCTCACAGCA 
CGAACACGGC CACCTAACGT TGACAGGCCG AGGATTAATT CTGTCATGGA CACATCTGTC 
TCAGTTTCCT GGTCACTAAG TCCTCACAGA CACACATCTA TTGCTCGTGT TCGTCTGTTT 
CTCCAGCACC CAACGGAGCC GCCCCAGCAG GCAGAGCAGG AGCCATCAGC AGGAGAATAT 
ATCTTCAGGG ATCTACGTCC TGGAGAAAAA TACAGCGTCC AAATTAGCAC CTTGAGCCGC 
TCTGACCCCA GCCACCCAGC CACAGAAAGC CTGCCCAGTG CACCTTTGAT AGCCTGGACA 
CGTCCACACC CACCTTCCAA CCTTACGGCA GCTCTTGTCA CAGCCAATTC TGTGCATCTA 
ATTTGGGAGC AACCTCCTCA TGGGTCAGTG GATGGCTATA TCTTCAATGT GACCAATAAT 
CACAGTACAA AGAGCCGGTA TGTTCCCAGT GGAAAGATGA CTTCATATAC AGTCCGTGAC 
TTGCAGGCCG GCCAACGCTA CCGGATAACA GTGACTGCAC TACGCAACAC TCCCCAGGGC 
CCAGTGAACA GTGAGCCCAA GACAGTGCGT CCACAGACAC TGCAAAGGGA GGGACCTCAG 
GAGCGTCGTT GGACCCCCCC TAGAATTCTG CGGAACAGGC CTCCTCCACA GCCACATCAG 
GAGGTGCGAC TGCTGACAGA AAGGGGTCCT CCTCCTGCTG ATACTCCACA GCCGCACCGA 
TTCACAGAGC TGAACGATGG GCGCCGTCGT ATTTCAGCAA GGTTCAGCCA TCGTACCAAT 
AAAGCTATTA ATGTTACAAA AGAGCCAGAC CCTCCAGTCA AAATTGAGAG CTCAGACGAA 
GCTGCTATTC CTGTGAGGCA GGCGCTGCAG CTACAGGACC CTGAGCAAAG AAGCAAAGAA 
GGAGAACGAC TGGGCTGCCT GGAGACCCCG TGTCTTAATG GAGGGACCTG TGTGAATGGA 
GACACTTGTG ACTGTCCTCT GGGATTTAAA GGGCAGCAAT GCCAGTTGGC CTGCAGAAAG 
GAGACCTTTT CCTGCACAAG ACTGATTTCT GAGACAACAT CACACCCAGT GTGGGAGGGA 
GGAATCTGCC ACCACCTGTA CAGAAGAGTT TACAAGGTAC AGAATGATGT GTGCAGCAGG 
GAGGTTTGTG AAGGTACCAG CAGAAGCAGA GCAAGTGAGT GGACTCTTTT GGTTACAATT 
ACAGAAAGCA GAATCCCTTC CAAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sned1 ( XM_002933337.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933383.2 Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X3, mRNA.

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CloneID	OXa16046
Clone ID Related Accession (Same CDS sequence)	XM_002933337.5 , XM_002933337.4
Accession Version	XM_002933337.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sushi, nidogen and EGF-like domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002933337.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA 
GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA 
TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG 
GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC 
TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA 
GGGTACAGTG GAAAGTATTG TGAGCAAGAA ACTAATGAGT GTCTGTCACA GCCATGTAAA 
AATGGGGGTA CCTGTCAGGA TTTGCCTGGG ACCTTCAAAT GTATTTGTCC TGAGGGTTTC 
ACTGGATCTT ATTGTGAGAC AGAGGTGGAT GGATGTGACT CTTCTCCGTG CCAGAATGGA 
GGCTTGTGTG AGAACCTGGC GGGTTCTTAT CTGTGCGTGT GTCCCCGCGG CTTTTTTGGC 
TATCACTGTC AGACAGTGAG TGACATGTGC CTGTTGAACC CCTGCGGTAA CCGTGGGGTA 
TGTGTGTCCA GCGCAGATGG ATTGATGAGC TGCACCTGCA GAACGGGGTA CACTGGAACC 
ATGTGTGAGA AAGAGCTGCT GCCGCCATTG TCACTGTCTC TGGGTGCGGT TGGAGAAAAC 
AGTGTTTGGG TGTTCTGGAC CCCCCCAGAA GATGGCACTG GGCATGTACT GGATGGGTTT 
GCAGTCAGTT ACACTGCCCC AGATGGCTTA TCTAGAACAG ACTTTGTAGA AAGAGCTCAC 
ACTGAACACG AGCTGCGGGG GCTTAGTCCT GGCCGCTCTT ATAACATCAC AATAGTAACA 
GTAAAGCGCA ACTTGCGGCA CAATGATATC AGTCGCCCCC TCTACCTCAC AGCACGAACA 
CGGCCACCTA ACGTTGACAG GCCGAGGATT AATTCTGTCA TGGACACATC TGTCTCAGTT 
TCCTGGTCAC TAAGTCCTCA CAGACACACA TCTATTGCTC GTGTTCGTCT GTTTCTCCAG 
CACCCAACGG AGCCGCCCCA GCAGGCAGAG CAGGAGCCAT CAGCAGGAGA ATATATCTTC 
AGGGATCTAC GTCCTGGAGA AAAATACAGC GTCCAAATTA GCACCTTGAG CCGCTCTGAC 
CCCAGCCACC CAGCCACAGA AAGCCTGCCC AGTGCACCTT TGATAGCCTG GACACGTCCA 
CACCCACCTT CCAACCTTAC GGCAGCTCTT GTCACAGCCA ATTCTGTGCA TCTAATTTGG 
GAGCAACCTC CTCATGGGTC AGTGGATGGC TATATCTTCA ATGTGACCAA TAATCACAGT 
ACAAAGAGCC GGTATGTTCC CAGTGGAAAG ATGACTTCAT ATACAGTCCG TGACTTGCAG 
GCCGGCCAAC GCTACCGGAT AACAGTGACT GCACTACGCA ACACTCCCCA GGGCCCAGTG 
AACAGTGAGC CCAAGACAGT GCGTCCACAG ACACTGCAAA GGGAGGGACC TCAGGAGCGT 
CGTTGGACCC CCCCTAGAAT TCTGCGGAAC AGGCCTCCTC CACAGCCACA TCAGGAGGTG 
CGACTGCTGA CAGAAAGGGG TCCTCCTCCT GCTGATACTC CACAGCCGCA CCGATTCACA 
GAGCTGAACG ATGGGCGCCG TCGTATTTCA GCAAGGTTCA GCCATCGTAC CAATAAAGCT 
ATTAATGTTA CAAAAGAGCC AGACCCTCCA GTCAAAATTG AGAGCTCAGA CGAAGCTGCT 
ATTCCTGTGA GGCAGGCGCT GCAGCTACAG GACCCTGAGC AAAGAAGCAA AGAAGGAGAA 
CGACTGGGCT GCCTGGAGAC CCCGTGTCTT AATGGAGGGA CCTGTGTGAA TGGAGACACT 
TGTGACTGTC CTCTGGGATT TAAAGGGCAG CAATGCCAGT TGGCCTGCAG AAAGGAGACC 
TTTTCCTGCA CAAGACTGAT TTCTGAGACA ACATCACACC CAGTGTGGGA GGGAGGAATC 
TGCCACCACC TGTACAGAAG AGTTTACAAG GTACAGAATG ATGTGTGCAG CAGGGAGGTT 
TGTGAAGGTA CCAGCAGAAG CAGAGCAAAA AGCAGAATCC CTTCCAAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933383.2
CDS	3785..8758
Translation	MRLPSLFWIFISMVWRAQGMVPLEDFYPFGTKEGDTVTRRQDDGGSGLLPISKAFTFFGDKHSGLYVNNNGVVSFLREVSQFTPVAFPISGDRRVVAAFWADVDNRRGGNVFYRETEDLVIVDRATRDVRTYFPELPDFHSTWVFIATWHRVTFYGGDSTSPVNTFQIVLITDGLRSFTIFNYETIEWTTGRHASSGGDSSGRGGIAAQAGFNAGDGWRYFNIPGSRTDDIARIANTTNVGVPGRWVFRIDNNHVQVGECRNATSVCWTLRPCQNGGRCIDDCVTGNPSYTCSCLSGFTGKHCHIDMGKCYSQPCQNDGTCVDEPGSFTCLCTSDFTGTLCETEVSPCTYMTCHNGGECEDHNGIAVCVCQRGYTGDNCDTELLPCSSSPCLNGGSCENIDAGFRCQCALGFTGTHCEKEPSACFSSPCMNNGVCKDVGAGYICVCVQGFTGMYCETEIRPCSSAPCLNRGVCRENGETYSCQCARRFTGKHCETEIRPCFSNPCMNMGTCEDMSEGYSCICTEGYTGIHCETELRPCYSSPCLNGALCKDFGITYSCVCAWGFTGARCETELHPCFSSPCLNGATCRDVGFAYSCQCAQGFTGMHCETEIKPCFSSPCLNGGTCEDLAISFACLCAERYTGLHCETEIQPCFSSPCLNRGICNNEGAFYSCTCAEGFTGENCEKEVSPCLSSPCLNGATCIDMGASYSCMCPQAFTGALCDTEIPSPCTSNPCENKGVCREAEGGYLCDCPAGFSGMHCEHRVSDDCPCRNGGRCADGNSTCHCPPGHFGLLCEYEVTSLPCSMGSQCPDGGSCMEYGGSYLCVCHTDYILSNHSIPSPCDSDPCLNGGTCESLDDAYTCACPRGFNGRLCEKVKPALCSLSPCRNGGTCKESREEYYCVCPYPFTGKHCETGMRGPCSSGPCNNGGTCFHYLGKYKCDCPPNFSGRHCEKAELDCGAPEQVKYAKMIVTSTLPGGRAEYHCNEGYVLSTPNNTSVCSEEGTWSLPPECEEIDECASHPCHNGASCKDRISHFICECQKGYSGKYCEQETNECLSQPCKNGGTCQDLPGTFKCICPEGFTGSYCETEVDGCDSSPCQNGGLCENLAGSYLCVCPRGFFGYHCQTVSDMCLLNPCGNRGVCVSSADGLMSCTCRTGYTGTMCEKELLPPLSLSLGAVGENSVWVFWTPPEDGTGHVLDGFAVSYTAPDGLSRTDFVERAHTEHELRGLSPGRSYNITIVTVKRNLRHNDISRPLYLTARTRPPNVDRPRINSVMDTSVSVSWSLSPHRHTSIARVRLFLQHPTEPPQQAEQEPSAGEYIFRDLRPGEKYSVQISTLSRSDPSHPATESLPSAPLIAWTRPHPPSNLTAALVTANSVHLIWEQPPHGSVDGYIFNVTNNHSTKSRYVPSGKMTSYTVRDLQAGQRYRITVTALRNTPQGPVNSEPKTVRPQTLQREGPQERRWTPPRILRNRPPPQPHQEVRLLTERGPPPADTPQPHRFTELNDGRRRISARFSHRTNKAINVTKEPDPPVKIESSDEAAIPVRQALQLQDPEQRSKEGERLGCLETPCLNGGTCVNGDTCDCPLGFKGQQCQLACRKETFSCTRLISETTSHPVWEGGICHHLYRRVYKVQNDVCSREVCEGTSRSRAKSRIPSKP

Target ORF information:

RefSeq Version	XM_002933337.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933337.4

ORF Insert Sequence:

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA 
GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA 
TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG 
GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC 
TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA 
GGGTACAGTG GAAAGTATTG TGAGCAAGAA ACTAATGAGT GTCTGTCACA GCCATGTAAA 
AATGGGGGTA CCTGTCAGGA TTTGCCTGGG ACCTTCAAAT GTATTTGTCC TGAGGGTTTC 
ACTGGATCTT ATTGTGAGAC AGAGGTGGAT GGATGTGACT CTTCTCCGTG CCAGAATGGA 
GGCTTGTGTG AGAACCTGGC GGGTTCTTAT CTGTGCGTGT GTCCCCGCGG CTTTTTTGGC 
TATCACTGTC AGACAGTGAG TGACATGTGC CTGTTGAACC CCTGCGGTAA CCGTGGGGTA 
TGTGTGTCCA GCGCAGATGG ATTGATGAGC TGCACCTGCA GAACGGGGTA CACTGGAACC 
ATGTGTGAGA AAGAGCTGCT GCCGCCATTG TCACTGTCTC TGGGTGCGGT TGGAGAAAAC 
AGTGTTTGGG TGTTCTGGAC CCCCCCAGAA GATGGCACTG GGCATGTACT GGATGGGTTT 
GCAGTCAGTT ACACTGCCCC AGATGGCTTA TCTAGAACAG ACTTTGTAGA AAGAGCTCAC 
ACTGAACACG AGCTGCGGGG GCTTAGTCCT GGCCGCTCTT ATAACATCAC AATAGTAACA 
GTAAAGCGCA ACTTGCGGCA CAATGATATC AGTCGCCCCC TCTACCTCAC AGCACGAACA 
CGGCCACCTA ACGTTGACAG GCCGAGGATT AATTCTGTCA TGGACACATC TGTCTCAGTT 
TCCTGGTCAC TAAGTCCTCA CAGACACACA TCTATTGCTC GTGTTCGTCT GTTTCTCCAG 
CACCCAACGG AGCCGCCCCA GCAGGCAGAG CAGGAGCCAT CAGCAGGAGA ATATATCTTC 
AGGGATCTAC GTCCTGGAGA AAAATACAGC GTCCAAATTA GCACCTTGAG CCGCTCTGAC 
CCCAGCCACC CAGCCACAGA AAGCCTGCCC AGTGCACCTT TGATAGCCTG GACACGTCCA 
CACCCACCTT CCAACCTTAC GGCAGCTCTT GTCACAGCCA ATTCTGTGCA TCTAATTTGG 
GAGCAACCTC CTCATGGGTC AGTGGATGGC TATATCTTCA ATGTGACCAA TAATCACAGT 
ACAAAGAGCC GGTATGTTCC CAGTGGAAAG ATGACTTCAT ATACAGTCCG TGACTTGCAG 
GCCGGCCAAC GCTACCGGAT AACAGTGACT GCACTACGCA ACACTCCCCA GGGCCCAGTG 
AACAGTGAGC CCAAGACAGT GCGTCCACAG ACACTGCAAA GGGAGGGACC TCAGGAGCGT 
CGTTGGACCC CCCCTAGAAT TCTGCGGAAC AGGCCTCCTC CACAGCCACA TCAGGAGGTG 
CGACTGCTGA CAGAAAGGGG TCCTCCTCCT GCTGATACTC CACAGCCGCA CCGATTCACA 
GAGCTGAACG ATGGGCGCCG TCGTATTTCA GCAAGGTTCA GCCATCGTAC CAATAAAGCT 
ATTAATGTTA CAAAAGAGCC AGACCCTCCA GTCAAAATTG AGAGCTCAGA CGAAGCTGCT 
ATTCCTGTGA GGCAGGCGCT GCAGCTACAG GACCCTGAGC AAAGAAGCAA AGAAGGAGAA 
CGACTGGGCT GCCTGGAGAC CCCGTGTCTT AATGGAGGGA CCTGTGTGAA TGGAGACACT 
TGTGACTGTC CTCTGGGATT TAAAGGGCAG CAATGCCAGT TGGCCTGCAG AAAGGAGACC 
TTTTCCTGCA CAAGACTGAT TTCTGAGACA ACATCACACC CAGTGTGGGA GGGAGGAATC 
TGCCACCACC TGTACAGAAG AGTTTACAAG GTACAGAATG ATGTGTGCAG CAGGGAGGTT 
TGTGAAGGTA CCAGCAGAAG CAGAGCAAAA AGCAGAATCC CTTCCAAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sned1 ( XM_012970829.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826283.1 Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa37188
Clone ID Related Accession (Same CDS sequence)	XM_012970829.3 , XM_012970829.2
Accession Version	XM_012970829.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sushi, nidogen and EGF-like domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970829.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA 
GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA 
TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG 
GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC 
TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA 
GGGTACAGTG GAAAGTATTG TGAGCAAGAA ACTAATGAGT GTCTGTCACA GCCATGTAAA 
AATGGGGGTA CCTGTCAGGA TTTGCCTGGG ACCTTCAAAT GTATTTGTCC TGAGGGTTTC 
ACTGGATCTT ATTGTGAGAC AGAGGTGGAT GGATGTGACT CTTCTCCGTG CCAGAATGGA 
GGCTTGTGTG AGAACCTGGC GGGTTCTTAT CTGTGCGTGT GTCCCCGCGG CTTTTTTGGC 
TATCACTGTC AGACAGTGAG TGACATGTGC CTGTTGAACC CCTGCGGTAA CCGTGGGGTA 
TGTGTGTCCA GCGCAGATGG ATTGATGAGC TGCACCTGCA GAACGGGGTA CACTGGAACC 
ATGTGTGAGA AAGAGCTGCT GCCGCCATTG TCACTGTCTC TGGGTGCGGT TGGAGAAAAC 
AGTGTTTGGG TGTTCTGGAC CCCCCCAGAA GATGGCACTG GGCATGTACT GGATGGGTTT 
GCAGTCAGTT ACACTGCCCC AGATGGCTTA TCTAGAACAG ACTTTGTAGA AAGAGCTCAC 
ACTGAACACG AGCTGCGGGG GCTTAGTCCT GGCCGCTCTT ATAACATCAC AATAGTAACA 
GTAAAGCGCA ACTTGCGGCA CAATGATATC AGTCGCCCCC TCTACCTCAC AGCACGAACA 
CGGCCACCTA ACGTTGACAG GCCGAGGATT AATTCTGTCA TGGACACATC TGTCTCAGTT 
TCCTGGTCAC TAAGTCCTCA CAGACACACA TCTATTGCTC GTGTTCGTCT GTTTCTCCAG 
CACCCAACGG AGCCGCCCCA GCAGGCAGAG CAGGAGCCAT CAGCAGGAGA ATATATCTTC 
AGGGATCTAC GTCCTGGAGA AAAATACAGC GTCCAAATTA GCACCTTGAG CCGCTCTGAC 
CCCAGCCACC CAGCCACAGA AAGCCTGCCC AGTGCACCTT TGATAGCCTG GACACGTCCA 
CACCCACCTT CCAACCTTAC GGCAGCTCTT GTCACAGCCA ATTCTGTGCA TCTAATTTGG 
GAGCAACCTC CTCATGGGTC AGTGGATGGC TATATCTTCA ATGTGACCAA TAATCACAGT 
ACAAAGAGCC GGTATGTTCC CAGTGGAAAG ATGACTTCAT ATACAGTCCG TGACTTGCAG 
GCCGGCCAAC GCTACCGGAT AACAGTGACT GCACTACGCA ACACTCCCCA GGGCCCAGTG 
AACAGTGAGC CCAAGACAGT GCGTCCACAG ACACTGCAAA GGGAGGGACC TCAGGAGCGT 
CGTTGGACCC CCCCTAGAAT TCTGCGGAAC AGGCCTCCTC CACAGCCACA TCAGGAGGTG 
CGACTGCTGA CAGAAAGGGG TCCTCCTCCT GCTGATACTC CACAGCCGCA CCGATTCACA 
GAGCTGAACG ATGGGCGCCG TCGTATTTCA GCAAGGTTCA GCCATCGTAC CAATAAAGCT 
ATTAATGTTA CAAAAGAGCC AGACCCTCCA GTCAAAATTG AGAGCTCAGA CGAAGCTGCT 
ATTCCTGTGA GGCAGGCGCT GCAGCTACAG GACCCTGAGC AAAGAAGCAA AGAAGGAGAA 
CGACTGGGCT GCCTGGAGAC CCCGTGTCTT AATGGAGGGA CCTGTGTGAA TGGAGACACT 
TGTGACTGTC CTCTGGGATT TAAAGGGCAG CAATGCCAGT TGGCCTGCAG AAAGGAGACC 
TTTTCCTGCA CAAGACTGAT TTCTGAGACA ACATCACACC CAGTGTGGGA GGGAGGAATC 
TGCCACCACC TGTACAGAAG AGTTTACAAG GTACAGAATG ATGTGTGCAG CAGGGAGGTT 
TGTGAAGGTA CCAGCAGAAG CAGAGCAAGT GAGTGGACTC TTTTGGTTAC AATTACAGAA 
AGCAGAATCC CTTCCAAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826283.1
CDS	3785..8788
Translation	MRLPSLFWIFISMVWRAQGMVPLEDFYPFGTKEGDTVTRRQDDGGSGLLPISKAFTFFGDKHSGLYVNNNGVVSFLREVSQFTPVAFPISGDRRVVAAFWADVDNRRGGNVFYRETEDLVIVDRATRDVRTYFPELPDFHSTWVFIATWHRVTFYGGDSTSPVNTFQIVLITDGLRSFTIFNYETIEWTTGRHASSGGDSSGRGGIAAQAGFNAGDGWRYFNIPGSRTDDIARIANTTNVGVPGRWVFRIDNNHVQVGECRNATSVCWTLRPCQNGGRCIDDCVTGNPSYTCSCLSGFTGKHCHIDMGKCYSQPCQNDGTCVDEPGSFTCLCTSDFTGTLCETEVSPCTYMTCHNGGECEDHNGIAVCVCQRGYTGDNCDTELLPCSSSPCLNGGSCENIDAGFRCQCALGFTGTHCEKEPSACFSSPCMNNGVCKDVGAGYICVCVQGFTGMYCETEIRPCSSAPCLNRGVCRENGETYSCQCARRFTGKHCETEIRPCFSNPCMNMGTCEDMSEGYSCICTEGYTGIHCETELRPCYSSPCLNGALCKDFGITYSCVCAWGFTGARCETELHPCFSSPCLNGATCRDVGFAYSCQCAQGFTGMHCETEIKPCFSSPCLNGGTCEDLAISFACLCAERYTGLHCETEIQPCFSSPCLNRGICNNEGAFYSCTCAEGFTGENCEKEVSPCLSSPCLNGATCIDMGASYSCMCPQAFTGALCDTEIPSPCTSNPCENKGVCREAEGGYLCDCPAGFSGMHCEHRVSDDCPCRNGGRCADGNSTCHCPPGHFGLLCEYEVTSLPCSMGSQCPDGGSCMEYGGSYLCVCHTDYILSNHSIPSPCDSDPCLNGGTCESLDDAYTCACPRGFNGRLCEKVKPALCSLSPCRNGGTCKESREEYYCVCPYPFTGKHCETGMRGPCSSGPCNNGGTCFHYLGKYKCDCPPNFSGRHCEKAELDCGAPEQVKYAKMIVTSTLPGGRAEYHCNEGYVLSTPNNTSVCSEEGTWSLPPECEEIDECASHPCHNGASCKDRISHFICECQKGYSGKYCEQETNECLSQPCKNGGTCQDLPGTFKCICPEGFTGSYCETEVDGCDSSPCQNGGLCENLAGSYLCVCPRGFFGYHCQTVSDMCLLNPCGNRGVCVSSADGLMSCTCRTGYTGTMCEKELLPPLSLSLGAVGENSVWVFWTPPEDGTGHVLDGFAVSYTAPDGLSRTDFVERAHTEHELRGLSPGRSYNITIVTVKRNLRHNDISRPLYLTARTRPPNVDRPRINSVMDTSVSVSWSLSPHRHTSIARVRLFLQHPTEPPQQAEQEPSAGEYIFRDLRPGEKYSVQISTLSRSDPSHPATESLPSAPLIAWTRPHPPSNLTAALVTANSVHLIWEQPPHGSVDGYIFNVTNNHSTKSRYVPSGKMTSYTVRDLQAGQRYRITVTALRNTPQGPVNSEPKTVRPQTLQREGPQERRWTPPRILRNRPPPQPHQEVRLLTERGPPPADTPQPHRFTELNDGRRRISARFSHRTNKAINVTKEPDPPVKIESSDEAAIPVRQALQLQDPEQRSKEGERLGCLETPCLNGGTCVNGDTCDCPLGFKGQQCQLACRKETFSCTRLISETTSHPVWEGGICHHLYRRVYKVQNDVCSREVCEGTSRSRASEWTLLVTITESRIPSKP

Target ORF information:

RefSeq Version	XM_012970829.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970829.2

ORF Insert Sequence:

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA 
GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA 
TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG 
GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC 
TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA 
GGGTACAGTG GAAAGTATTG TGAGCAAGAA ACTAATGAGT GTCTGTCACA GCCATGTAAA 
AATGGGGGTA CCTGTCAGGA TTTGCCTGGG ACCTTCAAAT GTATTTGTCC TGAGGGTTTC 
ACTGGATCTT ATTGTGAGAC AGAGGTGGAT GGATGTGACT CTTCTCCGTG CCAGAATGGA 
GGCTTGTGTG AGAACCTGGC GGGTTCTTAT CTGTGCGTGT GTCCCCGCGG CTTTTTTGGC 
TATCACTGTC AGACAGTGAG TGACATGTGC CTGTTGAACC CCTGCGGTAA CCGTGGGGTA 
TGTGTGTCCA GCGCAGATGG ATTGATGAGC TGCACCTGCA GAACGGGGTA CACTGGAACC 
ATGTGTGAGA AAGAGCTGCT GCCGCCATTG TCACTGTCTC TGGGTGCGGT TGGAGAAAAC 
AGTGTTTGGG TGTTCTGGAC CCCCCCAGAA GATGGCACTG GGCATGTACT GGATGGGTTT 
GCAGTCAGTT ACACTGCCCC AGATGGCTTA TCTAGAACAG ACTTTGTAGA AAGAGCTCAC 
ACTGAACACG AGCTGCGGGG GCTTAGTCCT GGCCGCTCTT ATAACATCAC AATAGTAACA 
GTAAAGCGCA ACTTGCGGCA CAATGATATC AGTCGCCCCC TCTACCTCAC AGCACGAACA 
CGGCCACCTA ACGTTGACAG GCCGAGGATT AATTCTGTCA TGGACACATC TGTCTCAGTT 
TCCTGGTCAC TAAGTCCTCA CAGACACACA TCTATTGCTC GTGTTCGTCT GTTTCTCCAG 
CACCCAACGG AGCCGCCCCA GCAGGCAGAG CAGGAGCCAT CAGCAGGAGA ATATATCTTC 
AGGGATCTAC GTCCTGGAGA AAAATACAGC GTCCAAATTA GCACCTTGAG CCGCTCTGAC 
CCCAGCCACC CAGCCACAGA AAGCCTGCCC AGTGCACCTT TGATAGCCTG GACACGTCCA 
CACCCACCTT CCAACCTTAC GGCAGCTCTT GTCACAGCCA ATTCTGTGCA TCTAATTTGG 
GAGCAACCTC CTCATGGGTC AGTGGATGGC TATATCTTCA ATGTGACCAA TAATCACAGT 
ACAAAGAGCC GGTATGTTCC CAGTGGAAAG ATGACTTCAT ATACAGTCCG TGACTTGCAG 
GCCGGCCAAC GCTACCGGAT AACAGTGACT GCACTACGCA ACACTCCCCA GGGCCCAGTG 
AACAGTGAGC CCAAGACAGT GCGTCCACAG ACACTGCAAA GGGAGGGACC TCAGGAGCGT 
CGTTGGACCC CCCCTAGAAT TCTGCGGAAC AGGCCTCCTC CACAGCCACA TCAGGAGGTG 
CGACTGCTGA CAGAAAGGGG TCCTCCTCCT GCTGATACTC CACAGCCGCA CCGATTCACA 
GAGCTGAACG ATGGGCGCCG TCGTATTTCA GCAAGGTTCA GCCATCGTAC CAATAAAGCT 
ATTAATGTTA CAAAAGAGCC AGACCCTCCA GTCAAAATTG AGAGCTCAGA CGAAGCTGCT 
ATTCCTGTGA GGCAGGCGCT GCAGCTACAG GACCCTGAGC AAAGAAGCAA AGAAGGAGAA 
CGACTGGGCT GCCTGGAGAC CCCGTGTCTT AATGGAGGGA CCTGTGTGAA TGGAGACACT 
TGTGACTGTC CTCTGGGATT TAAAGGGCAG CAATGCCAGT TGGCCTGCAG AAAGGAGACC 
TTTTCCTGCA CAAGACTGAT TTCTGAGACA ACATCACACC CAGTGTGGGA GGGAGGAATC 
TGCCACCACC TGTACAGAAG AGTTTACAAG GTACAGAATG ATGTGTGCAG CAGGGAGGTT 
TGTGAAGGTA CCAGCAGAAG CAGAGCAAGT GAGTGGACTC TTTTGGTTAC AATTACAGAA 
AGCAGAATCC CTTCCAAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sned1 ( XM_012970831.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826285.1 Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X2, mRNA.

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CloneID	OXa37189
Clone ID Related Accession (Same CDS sequence)	XM_012970831.3 , XM_012970831.2
Accession Version	XM_012970831.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sushi, nidogen and EGF-like domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970831.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGAAT TGGACTGTGG AGCCCCAGAG 
CAGGTAAAAT ATGCGAAGAT GATTGTGACA TCTACACTGC CGGGGGGTCG AGCAGAATAT 
CACTGTAATG AAGGCTATGT GCTCAGTACT CCAAACAACA CTAGTGTGTG CAGTGAGGAA 
GGGACATGGA GCTTGCCCCC AGAGTGTGAA GAAATAGACG AGTGTGCATC TCATCCCTGC 
CATAATGGGG CATCCTGCAA GGACCGGATT TCCCACTTTA TCTGTGAATG CCAGAAAGGG 
TACAGTGGAA AGTATTGTGA GCAAGAAACT AATGAGTGTC TGTCACAGCC ATGTAAAAAT 
GGGGGTACCT GTCAGGATTT GCCTGGGACC TTCAAATGTA TTTGTCCTGA GGGTTTCACT 
GGATCTTATT GTGAGACAGA GGTGGATGGA TGTGACTCTT CTCCGTGCCA GAATGGAGGC 
TTGTGTGAGA ACCTGGCGGG TTCTTATCTG TGCGTGTGTC CCCGCGGCTT TTTTGGCTAT 
CACTGTCAGA CAGTGAGTGA CATGTGCCTG TTGAACCCCT GCGGTAACCG TGGGGTATGT 
GTGTCCAGCG CAGATGGATT GATGAGCTGC ACCTGCAGAA CGGGGTACAC TGGAACCATG 
TGTGAGAAAG AGCTGCTGCC GCCATTGTCA CTGTCTCTGG GTGCGGTTGG AGAAAACAGT 
GTTTGGGTGT TCTGGACCCC CCCAGAAGAT GGCACTGGGC ATGTACTGGA TGGGTTTGCA 
GTCAGTTACA CTGCCCCAGA TGGCTTATCT AGAACAGACT TTGTAGAAAG AGCTCACACT 
GAACACGAGC TGCGGGGGCT TAGTCCTGGC CGCTCTTATA ACATCACAAT AGTAACAGTA 
AAGCGCAACT TGCGGCACAA TGATATCAGT CGCCCCCTCT ACCTCACAGC ACGAACACGG 
CCACCTAACG TTGACAGGCC GAGGATTAAT TCTGTCATGG ACACATCTGT CTCAGTTTCC 
TGGTCACTAA GTCCTCACAG ACACACATCT ATTGCTCGTG TTCGTCTGTT TCTCCAGCAC 
CCAACGGAGC CGCCCCAGCA GGCAGAGCAG GAGCCATCAG CAGGAGAATA TATCTTCAGG 
GATCTACGTC CTGGAGAAAA ATACAGCGTC CAAATTAGCA CCTTGAGCCG CTCTGACCCC 
AGCCACCCAG CCACAGAAAG CCTGCCCAGT GCACCTTTGA TAGCCTGGAC ACGTCCACAC 
CCACCTTCCA ACCTTACGGC AGCTCTTGTC ACAGCCAATT CTGTGCATCT AATTTGGGAG 
CAACCTCCTC ATGGGTCAGT GGATGGCTAT ATCTTCAATG TGACCAATAA TCACAGTACA 
AAGAGCCGGT ATGTTCCCAG TGGAAAGATG ACTTCATATA CAGTCCGTGA CTTGCAGGCC 
GGCCAACGCT ACCGGATAAC AGTGACTGCA CTACGCAACA CTCCCCAGGG CCCAGTGAAC 
AGTGAGCCCA AGACAGTGCG TCCACAGACA CTGCAAAGGG AGGGACCTCA GGAGCGTCGT 
TGGACCCCCC CTAGAATTCT GCGGAACAGG CCTCCTCCAC AGCCACATCA GGAGGTGCGA 
CTGCTGACAG AAAGGGGTCC TCCTCCTGCT GATACTCCAC AGCCGCACCG ATTCACAGAG 
CTGAACGATG GGCGCCGTCG TATTTCAGCA AGGTTCAGCC ATCGTACCAA TAAAGCTATT 
AATGTTACAA AAGAGCCAGA CCCTCCAGTC AAAATTGAGA GCTCAGACGA AGCTGCTATT 
CCTGTGAGGC AGGCGCTGCA GCTACAGGAC CCTGAGCAAA GAAGCAAAGA AGGAGAACGA 
CTGGGCTGCC TGGAGACCCC GTGTCTTAAT GGAGGGACCT GTGTGAATGG AGACACTTGT 
GACTGTCCTC TGGGATTTAA AGGGCAGCAA TGCCAGTTGG CCTGCAGAAA GGAGACCTTT 
TCCTGCACAA GACTGATTTC TGAGACAACA TCACACCCAG TGTGGGAGGG AGGAATCTGC 
CACCACCTGT ACAGAAGAGT TTACAAGGTA CAGAATGATG TGTGCAGCAG GGAGGTTTGT 
GAAGGTACCA GCAGAAGCAG AGCAAGTGAG TGGACTCTTT TGGTTACAAT TACAGAAAGC 
AGAATCCCTT CCAAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826285.1
CDS	3785..8785
Translation	MRLPSLFWIFISMVWRAQGMVPLEDFYPFGTKEGDTVTRRQDDGGSGLLPISKAFTFFGDKHSGLYVNNNGVVSFLREVSQFTPVAFPISGDRRVVAAFWADVDNRRGGNVFYRETEDLVIVDRATRDVRTYFPELPDFHSTWVFIATWHRVTFYGGDSTSPVNTFQIVLITDGLRSFTIFNYETIEWTTGRHASSGGDSSGRGGIAAQAGFNAGDGWRYFNIPGSRTDDIARIANTTNVGVPGRWVFRIDNNHVQVGECRNATSVCWTLRPCQNGGRCIDDCVTGNPSYTCSCLSGFTGKHCHIDMGKCYSQPCQNDGTCVDEPGSFTCLCTSDFTGTLCETEVSPCTYMTCHNGGECEDHNGIAVCVCQRGYTGDNCDTELLPCSSSPCLNGGSCENIDAGFRCQCALGFTGTHCEKEPSACFSSPCMNNGVCKDVGAGYICVCVQGFTGMYCETEIRPCSSAPCLNRGVCRENGETYSCQCARRFTGKHCETEIRPCFSNPCMNMGTCEDMSEGYSCICTEGYTGIHCETELRPCYSSPCLNGALCKDFGITYSCVCAWGFTGARCETELHPCFSSPCLNGATCRDVGFAYSCQCAQGFTGMHCETEIKPCFSSPCLNGGTCEDLAISFACLCAERYTGLHCETEIQPCFSSPCLNRGICNNEGAFYSCTCAEGFTGENCEKEVSPCLSSPCLNGATCIDMGASYSCMCPQAFTGALCDTEIPSPCTSNPCENKGVCREAEGGYLCDCPAGFSGMHCEHRVSDDCPCRNGGRCADGNSTCHCPPGHFGLLCEYEVTSLPCSMGSQCPDGGSCMEYGGSYLCVCHTDYILSNHSIPSPCDSDPCLNGGTCESLDDAYTCACPRGFNGRLCEKVKPALCSLSPCRNGGTCKESREEYYCVCPYPFTGKHCETGMRGPCSSGPCNNGGTCFHYLGKYKCDCPPNFSGRHCEKELDCGAPEQVKYAKMIVTSTLPGGRAEYHCNEGYVLSTPNNTSVCSEEGTWSLPPECEEIDECASHPCHNGASCKDRISHFICECQKGYSGKYCEQETNECLSQPCKNGGTCQDLPGTFKCICPEGFTGSYCETEVDGCDSSPCQNGGLCENLAGSYLCVCPRGFFGYHCQTVSDMCLLNPCGNRGVCVSSADGLMSCTCRTGYTGTMCEKELLPPLSLSLGAVGENSVWVFWTPPEDGTGHVLDGFAVSYTAPDGLSRTDFVERAHTEHELRGLSPGRSYNITIVTVKRNLRHNDISRPLYLTARTRPPNVDRPRINSVMDTSVSVSWSLSPHRHTSIARVRLFLQHPTEPPQQAEQEPSAGEYIFRDLRPGEKYSVQISTLSRSDPSHPATESLPSAPLIAWTRPHPPSNLTAALVTANSVHLIWEQPPHGSVDGYIFNVTNNHSTKSRYVPSGKMTSYTVRDLQAGQRYRITVTALRNTPQGPVNSEPKTVRPQTLQREGPQERRWTPPRILRNRPPPQPHQEVRLLTERGPPPADTPQPHRFTELNDGRRRISARFSHRTNKAINVTKEPDPPVKIESSDEAAIPVRQALQLQDPEQRSKEGERLGCLETPCLNGGTCVNGDTCDCPLGFKGQQCQLACRKETFSCTRLISETTSHPVWEGGICHHLYRRVYKVQNDVCSREVCEGTSRSRASEWTLLVTITESRIPSKP

Target ORF information:

RefSeq Version	XM_012970831.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970831.2

ORF Insert Sequence:

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGAAT TGGACTGTGG AGCCCCAGAG 
CAGGTAAAAT ATGCGAAGAT GATTGTGACA TCTACACTGC CGGGGGGTCG AGCAGAATAT 
CACTGTAATG AAGGCTATGT GCTCAGTACT CCAAACAACA CTAGTGTGTG CAGTGAGGAA 
GGGACATGGA GCTTGCCCCC AGAGTGTGAA GAAATAGACG AGTGTGCATC TCATCCCTGC 
CATAATGGGG CATCCTGCAA GGACCGGATT TCCCACTTTA TCTGTGAATG CCAGAAAGGG 
TACAGTGGAA AGTATTGTGA GCAAGAAACT AATGAGTGTC TGTCACAGCC ATGTAAAAAT 
GGGGGTACCT GTCAGGATTT GCCTGGGACC TTCAAATGTA TTTGTCCTGA GGGTTTCACT 
GGATCTTATT GTGAGACAGA GGTGGATGGA TGTGACTCTT CTCCGTGCCA GAATGGAGGC 
TTGTGTGAGA ACCTGGCGGG TTCTTATCTG TGCGTGTGTC CCCGCGGCTT TTTTGGCTAT 
CACTGTCAGA CAGTGAGTGA CATGTGCCTG TTGAACCCCT GCGGTAACCG TGGGGTATGT 
GTGTCCAGCG CAGATGGATT GATGAGCTGC ACCTGCAGAA CGGGGTACAC TGGAACCATG 
TGTGAGAAAG AGCTGCTGCC GCCATTGTCA CTGTCTCTGG GTGCGGTTGG AGAAAACAGT 
GTTTGGGTGT TCTGGACCCC CCCAGAAGAT GGCACTGGGC ATGTACTGGA TGGGTTTGCA 
GTCAGTTACA CTGCCCCAGA TGGCTTATCT AGAACAGACT TTGTAGAAAG AGCTCACACT 
GAACACGAGC TGCGGGGGCT TAGTCCTGGC CGCTCTTATA ACATCACAAT AGTAACAGTA 
AAGCGCAACT TGCGGCACAA TGATATCAGT CGCCCCCTCT ACCTCACAGC ACGAACACGG 
CCACCTAACG TTGACAGGCC GAGGATTAAT TCTGTCATGG ACACATCTGT CTCAGTTTCC 
TGGTCACTAA GTCCTCACAG ACACACATCT ATTGCTCGTG TTCGTCTGTT TCTCCAGCAC 
CCAACGGAGC CGCCCCAGCA GGCAGAGCAG GAGCCATCAG CAGGAGAATA TATCTTCAGG 
GATCTACGTC CTGGAGAAAA ATACAGCGTC CAAATTAGCA CCTTGAGCCG CTCTGACCCC 
AGCCACCCAG CCACAGAAAG CCTGCCCAGT GCACCTTTGA TAGCCTGGAC ACGTCCACAC 
CCACCTTCCA ACCTTACGGC AGCTCTTGTC ACAGCCAATT CTGTGCATCT AATTTGGGAG 
CAACCTCCTC ATGGGTCAGT GGATGGCTAT ATCTTCAATG TGACCAATAA TCACAGTACA 
AAGAGCCGGT ATGTTCCCAG TGGAAAGATG ACTTCATATA CAGTCCGTGA CTTGCAGGCC 
GGCCAACGCT ACCGGATAAC AGTGACTGCA CTACGCAACA CTCCCCAGGG CCCAGTGAAC 
AGTGAGCCCA AGACAGTGCG TCCACAGACA CTGCAAAGGG AGGGACCTCA GGAGCGTCGT 
TGGACCCCCC CTAGAATTCT GCGGAACAGG CCTCCTCCAC AGCCACATCA GGAGGTGCGA 
CTGCTGACAG AAAGGGGTCC TCCTCCTGCT GATACTCCAC AGCCGCACCG ATTCACAGAG 
CTGAACGATG GGCGCCGTCG TATTTCAGCA AGGTTCAGCC ATCGTACCAA TAAAGCTATT 
AATGTTACAA AAGAGCCAGA CCCTCCAGTC AAAATTGAGA GCTCAGACGA AGCTGCTATT 
CCTGTGAGGC AGGCGCTGCA GCTACAGGAC CCTGAGCAAA GAAGCAAAGA AGGAGAACGA 
CTGGGCTGCC TGGAGACCCC GTGTCTTAAT GGAGGGACCT GTGTGAATGG AGACACTTGT 
GACTGTCCTC TGGGATTTAA AGGGCAGCAA TGCCAGTTGG CCTGCAGAAA GGAGACCTTT 
TCCTGCACAA GACTGATTTC TGAGACAACA TCACACCCAG TGTGGGAGGG AGGAATCTGC 
CACCACCTGT ACAGAAGAGT TTACAAGGTA CAGAATGATG TGTGCAGCAG GGAGGTTTGT 
GAAGGTACCA GCAGAAGCAG AGCAAGTGAG TGGACTCTTT TGGTTACAAT TACAGAAAGC 
AGAATCCCTT CCAAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sned1 ( XM_012970833.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826287.1 Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X5, mRNA.

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CloneID	OXa37191
Clone ID Related Accession (Same CDS sequence)	XM_012970833.3 , XM_012970833.2
Accession Version	XM_012970833.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sushi, nidogen and EGF-like domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970833.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA 
GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA 
TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG 
GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAACTA ATGAGTGTCT GTCACAGCCA 
TGTAAAAATG GGGGTACCTG TCAGGATTTG CCTGGGACCT TCAAATGTAT TTGTCCTGAG 
GGTTTCACTG GATCTTATTG TGAGACAGAG GTGGATGGAT GTGACTCTTC TCCGTGCCAG 
AATGGAGGCT TGTGTGAGAA CCTGGCGGGT TCTTATCTGT GCGTGTGTCC CCGCGGCTTT 
TTTGGCTATC ACTGTCAGAC AGTGAGTGAC ATGTGCCTGT TGAACCCCTG CGGTAACCGT 
GGGGTATGTG TGTCCAGCGC AGATGGATTG ATGAGCTGCA CCTGCAGAAC GGGGTACACT 
GGAACCATGT GTGAGAAAGA GCTGCTGCCG CCATTGTCAC TGTCTCTGGG TGCGGTTGGA 
GAAAACAGTG TTTGGGTGTT CTGGACCCCC CCAGAAGATG GCACTGGGCA TGTACTGGAT 
GGGTTTGCAG TCAGTTACAC TGCCCCAGAT GGCTTATCTA GAACAGACTT TGTAGAAAGA 
GCTCACACTG AACACGAGCT GCGGGGGCTT AGTCCTGGCC GCTCTTATAA CATCACAATA 
GTAACAGTAA AGCGCAACTT GCGGCACAAT GATATCAGTC GCCCCCTCTA CCTCACAGCA 
CGAACACGGC CACCTAACGT TGACAGGCCG AGGATTAATT CTGTCATGGA CACATCTGTC 
TCAGTTTCCT GGTCACTAAG TCCTCACAGA CACACATCTA TTGCTCGTGT TCGTCTGTTT 
CTCCAGCACC CAACGGAGCC GCCCCAGCAG GCAGAGCAGG AGCCATCAGC AGGAGAATAT 
ATCTTCAGGG ATCTACGTCC TGGAGAAAAA TACAGCGTCC AAATTAGCAC CTTGAGCCGC 
TCTGACCCCA GCCACCCAGC CACAGAAAGC CTGCCCAGTG CACCTTTGAT AGCCTGGACA 
CGTCCACACC CACCTTCCAA CCTTACGGCA GCTCTTGTCA CAGCCAATTC TGTGCATCTA 
ATTTGGGAGC AACCTCCTCA TGGGTCAGTG GATGGCTATA TCTTCAATGT GACCAATAAT 
CACAGTACAA AGAGCCGGTA TGTTCCCAGT GGAAAGATGA CTTCATATAC AGTCCGTGAC 
TTGCAGGCCG GCCAACGCTA CCGGATAACA GTGACTGCAC TACGCAACAC TCCCCAGGGC 
CCAGTGAACA GTGAGCCCAA GACAGTGCGT CCACAGACAC TGCAAAGGGA GGGACCTCAG 
GAGCGTCGTT GGACCCCCCC TAGAATTCTG CGGAACAGGC CTCCTCCACA GCCACATCAG 
GAGGTGCGAC TGCTGACAGA AAGGGGTCCT CCTCCTGCTG ATACTCCACA GCCGCACCGA 
TTCACAGAGC TGAACGATGG GCGCCGTCGT ATTTCAGCAA GGTTCAGCCA TCGTACCAAT 
AAAGCTATTA ATGTTACAAA AGAGCCAGAC CCTCCAGTCA AAATTGAGAG CTCAGACGAA 
GCTGCTATTC CTGTGAGGCA GGCGCTGCAG CTACAGGACC CTGAGCAAAG AAGCAAAGAA 
GGAGAACGAC TGGGCTGCCT GGAGACCCCG TGTCTTAATG GAGGGACCTG TGTGAATGGA 
GACACTTGTG ACTGTCCTCT GGGATTTAAA GGGCAGCAAT GCCAGTTGGC CTGCAGAAAG 
GAGACCTTTT CCTGCACAAG ACTGATTTCT GAGACAACAT CACACCCAGT GTGGGAGGGA 
GGAATCTGCC ACCACCTGTA CAGAAGAGTT TACAAGGTAC AGAATGATGT GTGCAGCAGG 
GAGGTTTGTG AAGGTACCAG CAGAAGCAGA GCAAGTGAGT GGACTCTTTT GGTTACAATT 
ACAGAAAGCA GAATCCCTTC CAAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826287.1
CDS	3785..8674
Translation	MRLPSLFWIFISMVWRAQGMVPLEDFYPFGTKEGDTVTRRQDDGGSGLLPISKAFTFFGDKHSGLYVNNNGVVSFLREVSQFTPVAFPISGDRRVVAAFWADVDNRRGGNVFYRETEDLVIVDRATRDVRTYFPELPDFHSTWVFIATWHRVTFYGGDSTSPVNTFQIVLITDGLRSFTIFNYETIEWTTGRHASSGGDSSGRGGIAAQAGFNAGDGWRYFNIPGSRTDDIARIANTTNVGVPGRWVFRIDNNHVQVGECRNATSVCWTLRPCQNGGRCIDDCVTGNPSYTCSCLSGFTGKHCHIDMGKCYSQPCQNDGTCVDEPGSFTCLCTSDFTGTLCETEVSPCTYMTCHNGGECEDHNGIAVCVCQRGYTGDNCDTELLPCSSSPCLNGGSCENIDAGFRCQCALGFTGTHCEKEPSACFSSPCMNNGVCKDVGAGYICVCVQGFTGMYCETEIRPCSSAPCLNRGVCRENGETYSCQCARRFTGKHCETEIRPCFSNPCMNMGTCEDMSEGYSCICTEGYTGIHCETELRPCYSSPCLNGALCKDFGITYSCVCAWGFTGARCETELHPCFSSPCLNGATCRDVGFAYSCQCAQGFTGMHCETEIKPCFSSPCLNGGTCEDLAISFACLCAERYTGLHCETEIQPCFSSPCLNRGICNNEGAFYSCTCAEGFTGENCEKEVSPCLSSPCLNGATCIDMGASYSCMCPQAFTGALCDTEIPSPCTSNPCENKGVCREAEGGYLCDCPAGFSGMHCEHRVSDDCPCRNGGRCADGNSTCHCPPGHFGLLCEYEVTSLPCSMGSQCPDGGSCMEYGGSYLCVCHTDYILSNHSIPSPCDSDPCLNGGTCESLDDAYTCACPRGFNGRLCEKVKPALCSLSPCRNGGTCKESREEYYCVCPYPFTGKHCETGMRGPCSSGPCNNGGTCFHYLGKYKCDCPPNFSGRHCEKAELDCGAPEQVKYAKMIVTSTLPGGRAEYHCNEGYVLSTPNNTSVCSEEGTWSLPPECEETNECLSQPCKNGGTCQDLPGTFKCICPEGFTGSYCETEVDGCDSSPCQNGGLCENLAGSYLCVCPRGFFGYHCQTVSDMCLLNPCGNRGVCVSSADGLMSCTCRTGYTGTMCEKELLPPLSLSLGAVGENSVWVFWTPPEDGTGHVLDGFAVSYTAPDGLSRTDFVERAHTEHELRGLSPGRSYNITIVTVKRNLRHNDISRPLYLTARTRPPNVDRPRINSVMDTSVSVSWSLSPHRHTSIARVRLFLQHPTEPPQQAEQEPSAGEYIFRDLRPGEKYSVQISTLSRSDPSHPATESLPSAPLIAWTRPHPPSNLTAALVTANSVHLIWEQPPHGSVDGYIFNVTNNHSTKSRYVPSGKMTSYTVRDLQAGQRYRITVTALRNTPQGPVNSEPKTVRPQTLQREGPQERRWTPPRILRNRPPPQPHQEVRLLTERGPPPADTPQPHRFTELNDGRRRISARFSHRTNKAINVTKEPDPPVKIESSDEAAIPVRQALQLQDPEQRSKEGERLGCLETPCLNGGTCVNGDTCDCPLGFKGQQCQLACRKETFSCTRLISETTSHPVWEGGICHHLYRRVYKVQNDVCSREVCEGTSRSRASEWTLLVTITESRIPSKP

Target ORF information:

RefSeq Version	XM_012970833.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970833.2

ORF Insert Sequence:

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA 
GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA 
TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG 
GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAACTA ATGAGTGTCT GTCACAGCCA 
TGTAAAAATG GGGGTACCTG TCAGGATTTG CCTGGGACCT TCAAATGTAT TTGTCCTGAG 
GGTTTCACTG GATCTTATTG TGAGACAGAG GTGGATGGAT GTGACTCTTC TCCGTGCCAG 
AATGGAGGCT TGTGTGAGAA CCTGGCGGGT TCTTATCTGT GCGTGTGTCC CCGCGGCTTT 
TTTGGCTATC ACTGTCAGAC AGTGAGTGAC ATGTGCCTGT TGAACCCCTG CGGTAACCGT 
GGGGTATGTG TGTCCAGCGC AGATGGATTG ATGAGCTGCA CCTGCAGAAC GGGGTACACT 
GGAACCATGT GTGAGAAAGA GCTGCTGCCG CCATTGTCAC TGTCTCTGGG TGCGGTTGGA 
GAAAACAGTG TTTGGGTGTT CTGGACCCCC CCAGAAGATG GCACTGGGCA TGTACTGGAT 
GGGTTTGCAG TCAGTTACAC TGCCCCAGAT GGCTTATCTA GAACAGACTT TGTAGAAAGA 
GCTCACACTG AACACGAGCT GCGGGGGCTT AGTCCTGGCC GCTCTTATAA CATCACAATA 
GTAACAGTAA AGCGCAACTT GCGGCACAAT GATATCAGTC GCCCCCTCTA CCTCACAGCA 
CGAACACGGC CACCTAACGT TGACAGGCCG AGGATTAATT CTGTCATGGA CACATCTGTC 
TCAGTTTCCT GGTCACTAAG TCCTCACAGA CACACATCTA TTGCTCGTGT TCGTCTGTTT 
CTCCAGCACC CAACGGAGCC GCCCCAGCAG GCAGAGCAGG AGCCATCAGC AGGAGAATAT 
ATCTTCAGGG ATCTACGTCC TGGAGAAAAA TACAGCGTCC AAATTAGCAC CTTGAGCCGC 
TCTGACCCCA GCCACCCAGC CACAGAAAGC CTGCCCAGTG CACCTTTGAT AGCCTGGACA 
CGTCCACACC CACCTTCCAA CCTTACGGCA GCTCTTGTCA CAGCCAATTC TGTGCATCTA 
ATTTGGGAGC AACCTCCTCA TGGGTCAGTG GATGGCTATA TCTTCAATGT GACCAATAAT 
CACAGTACAA AGAGCCGGTA TGTTCCCAGT GGAAAGATGA CTTCATATAC AGTCCGTGAC 
TTGCAGGCCG GCCAACGCTA CCGGATAACA GTGACTGCAC TACGCAACAC TCCCCAGGGC 
CCAGTGAACA GTGAGCCCAA GACAGTGCGT CCACAGACAC TGCAAAGGGA GGGACCTCAG 
GAGCGTCGTT GGACCCCCCC TAGAATTCTG CGGAACAGGC CTCCTCCACA GCCACATCAG 
GAGGTGCGAC TGCTGACAGA AAGGGGTCCT CCTCCTGCTG ATACTCCACA GCCGCACCGA 
TTCACAGAGC TGAACGATGG GCGCCGTCGT ATTTCAGCAA GGTTCAGCCA TCGTACCAAT 
AAAGCTATTA ATGTTACAAA AGAGCCAGAC CCTCCAGTCA AAATTGAGAG CTCAGACGAA 
GCTGCTATTC CTGTGAGGCA GGCGCTGCAG CTACAGGACC CTGAGCAAAG AAGCAAAGAA 
GGAGAACGAC TGGGCTGCCT GGAGACCCCG TGTCTTAATG GAGGGACCTG TGTGAATGGA 
GACACTTGTG ACTGTCCTCT GGGATTTAAA GGGCAGCAAT GCCAGTTGGC CTGCAGAAAG 
GAGACCTTTT CCTGCACAAG ACTGATTTCT GAGACAACAT CACACCCAGT GTGGGAGGGA 
GGAATCTGCC ACCACCTGTA CAGAAGAGTT TACAAGGTAC AGAATGATGT GTGCAGCAGG 
GAGGTTTGTG AAGGTACCAG CAGAAGCAGA GCAAGTGAGT GGACTCTTTT GGTTACAATT 
ACAGAAAGCA GAATCCCTTC CAAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sned1 ( XM_012970834.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826288.1 Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X4, mRNA.

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CloneID	OXa37192
Clone ID Related Accession (Same CDS sequence)	XM_012970834.2
Accession Version	XM_012970834.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sushi, nidogen and EGF-like domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970834.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA 
GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA 
TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG 
GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC 
TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA 
GGGTACAGTG GAAAGTATTG TGAGCAAGAG GTGGATGGAT GTGACTCTTC TCCGTGCCAG 
AATGGAGGCT TGTGTGAGAA CCTGGCGGGT TCTTATCTGT GCGTGTGTCC CCGCGGCTTT 
TTTGGCTATC ACTGTCAGAC AGTGAGTGAC ATGTGCCTGT TGAACCCCTG CGGTAACCGT 
GGGGTATGTG TGTCCAGCGC AGATGGATTG ATGAGCTGCA CCTGCAGAAC GGGGTACACT 
GGAACCATGT GTGAGAAAGA GCTGCTGCCG CCATTGTCAC TGTCTCTGGG TGCGGTTGGA 
GAAAACAGTG TTTGGGTGTT CTGGACCCCC CCAGAAGATG GCACTGGGCA TGTACTGGAT 
GGGTTTGCAG TCAGTTACAC TGCCCCAGAT GGCTTATCTA GAACAGACTT TGTAGAAAGA 
GCTCACACTG AACACGAGCT GCGGGGGCTT AGTCCTGGCC GCTCTTATAA CATCACAATA 
GTAACAGTAA AGCGCAACTT GCGGCACAAT GATATCAGTC GCCCCCTCTA CCTCACAGCA 
CGAACACGGC CACCTAACGT TGACAGGCCG AGGATTAATT CTGTCATGGA CACATCTGTC 
TCAGTTTCCT GGTCACTAAG TCCTCACAGA CACACATCTA TTGCTCGTGT TCGTCTGTTT 
CTCCAGCACC CAACGGAGCC GCCCCAGCAG GCAGAGCAGG AGCCATCAGC AGGAGAATAT 
ATCTTCAGGG ATCTACGTCC TGGAGAAAAA TACAGCGTCC AAATTAGCAC CTTGAGCCGC 
TCTGACCCCA GCCACCCAGC CACAGAAAGC CTGCCCAGTG CACCTTTGAT AGCCTGGACA 
CGTCCACACC CACCTTCCAA CCTTACGGCA GCTCTTGTCA CAGCCAATTC TGTGCATCTA 
ATTTGGGAGC AACCTCCTCA TGGGTCAGTG GATGGCTATA TCTTCAATGT GACCAATAAT 
CACAGTACAA AGAGCCGGTA TGTTCCCAGT GGAAAGATGA CTTCATATAC AGTCCGTGAC 
TTGCAGGCCG GCCAACGCTA CCGGATAACA GTGACTGCAC TACGCAACAC TCCCCAGGGC 
CCAGTGAACA GTGAGCCCAA GACAGTGCGT CCACAGACAC TGCAAAGGGA GGGACCTCAG 
GAGCGTCGTT GGACCCCCCC TAGAATTCTG CGGAACAGGC CTCCTCCACA GCCACATCAG 
GAGGTGCGAC TGCTGACAGA AAGGGGTCCT CCTCCTGCTG ATACTCCACA GCCGCACCGA 
TTCACAGAGC TGAACGATGG GCGCCGTCGT ATTTCAGCAA GGTTCAGCCA TCGTACCAAT 
AAAGCTATTA ATGTTACAAA AGAGCCAGAC CCTCCAGTCA AAATTGAGAG CTCAGACGAA 
GCTGCTATTC CTGTGAGGCA GGCGCTGCAG CTACAGGACC CTGAGCAAAG AAGCAAAGAA 
GGAGAACGAC TGGGCTGCCT GGAGACCCCG TGTCTTAATG GAGGGACCTG TGTGAATGGA 
GACACTTGTG ACTGTCCTCT GGGATTTAAA GGGCAGCAAT GCCAGTTGGC CTGCAGAAAG 
GAGACCTTTT CCTGCACAAG ACTGATTTCT GAGACAACAT CACACCCAGT GTGGGAGGGA 
GGAATCTGCC ACCACCTGTA CAGAAGAGTT TACAAGGTAC AGAATGATGT GTGCAGCAGG 
GAGGTTTGTG AAGGTACCAG CAGAAGCAGA GCAAGTGAGT GGACTCTTTT GGTTACAATT 
ACAGAAAGCA GAATCCCTTC CAAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826288.1
CDS	3785..8674
Translation	MRLPSLFWIFISMVWRAQGMVPLEDFYPFGTKEGDTVTRRQDDGGSGLLPISKAFTFFGDKHSGLYVNNNGVVSFLREVSQFTPVAFPISGDRRVVAAFWADVDNRRGGNVFYRETEDLVIVDRATRDVRTYFPELPDFHSTWVFIATWHRVTFYGGDSTSPVNTFQIVLITDGLRSFTIFNYETIEWTTGRHASSGGDSSGRGGIAAQAGFNAGDGWRYFNIPGSRTDDIARIANTTNVGVPGRWVFRIDNNHVQVGECRNATSVCWTLRPCQNGGRCIDDCVTGNPSYTCSCLSGFTGKHCHIDMGKCYSQPCQNDGTCVDEPGSFTCLCTSDFTGTLCETEVSPCTYMTCHNGGECEDHNGIAVCVCQRGYTGDNCDTELLPCSSSPCLNGGSCENIDAGFRCQCALGFTGTHCEKEPSACFSSPCMNNGVCKDVGAGYICVCVQGFTGMYCETEIRPCSSAPCLNRGVCRENGETYSCQCARRFTGKHCETEIRPCFSNPCMNMGTCEDMSEGYSCICTEGYTGIHCETELRPCYSSPCLNGALCKDFGITYSCVCAWGFTGARCETELHPCFSSPCLNGATCRDVGFAYSCQCAQGFTGMHCETEIKPCFSSPCLNGGTCEDLAISFACLCAERYTGLHCETEIQPCFSSPCLNRGICNNEGAFYSCTCAEGFTGENCEKEVSPCLSSPCLNGATCIDMGASYSCMCPQAFTGALCDTEIPSPCTSNPCENKGVCREAEGGYLCDCPAGFSGMHCEHRVSDDCPCRNGGRCADGNSTCHCPPGHFGLLCEYEVTSLPCSMGSQCPDGGSCMEYGGSYLCVCHTDYILSNHSIPSPCDSDPCLNGGTCESLDDAYTCACPRGFNGRLCEKVKPALCSLSPCRNGGTCKESREEYYCVCPYPFTGKHCETGMRGPCSSGPCNNGGTCFHYLGKYKCDCPPNFSGRHCEKAELDCGAPEQVKYAKMIVTSTLPGGRAEYHCNEGYVLSTPNNTSVCSEEGTWSLPPECEEIDECASHPCHNGASCKDRISHFICECQKGYSGKYCEQEVDGCDSSPCQNGGLCENLAGSYLCVCPRGFFGYHCQTVSDMCLLNPCGNRGVCVSSADGLMSCTCRTGYTGTMCEKELLPPLSLSLGAVGENSVWVFWTPPEDGTGHVLDGFAVSYTAPDGLSRTDFVERAHTEHELRGLSPGRSYNITIVTVKRNLRHNDISRPLYLTARTRPPNVDRPRINSVMDTSVSVSWSLSPHRHTSIARVRLFLQHPTEPPQQAEQEPSAGEYIFRDLRPGEKYSVQISTLSRSDPSHPATESLPSAPLIAWTRPHPPSNLTAALVTANSVHLIWEQPPHGSVDGYIFNVTNNHSTKSRYVPSGKMTSYTVRDLQAGQRYRITVTALRNTPQGPVNSEPKTVRPQTLQREGPQERRWTPPRILRNRPPPQPHQEVRLLTERGPPPADTPQPHRFTELNDGRRRISARFSHRTNKAINVTKEPDPPVKIESSDEAAIPVRQALQLQDPEQRSKEGERLGCLETPCLNGGTCVNGDTCDCPLGFKGQQCQLACRKETFSCTRLISETTSHPVWEGGICHHLYRRVYKVQNDVCSREVCEGTSRSRASEWTLLVTITESRIPSKP

Target ORF information:

RefSeq Version	XM_012970834.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970834.2

ORF Insert Sequence:

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA 
GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA 
TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG 
GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC 
TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA 
GGGTACAGTG GAAAGTATTG TGAGCAAGAG GTGGATGGAT GTGACTCTTC TCCGTGCCAG 
AATGGAGGCT TGTGTGAGAA CCTGGCGGGT TCTTATCTGT GCGTGTGTCC CCGCGGCTTT 
TTTGGCTATC ACTGTCAGAC AGTGAGTGAC ATGTGCCTGT TGAACCCCTG CGGTAACCGT 
GGGGTATGTG TGTCCAGCGC AGATGGATTG ATGAGCTGCA CCTGCAGAAC GGGGTACACT 
GGAACCATGT GTGAGAAAGA GCTGCTGCCG CCATTGTCAC TGTCTCTGGG TGCGGTTGGA 
GAAAACAGTG TTTGGGTGTT CTGGACCCCC CCAGAAGATG GCACTGGGCA TGTACTGGAT 
GGGTTTGCAG TCAGTTACAC TGCCCCAGAT GGCTTATCTA GAACAGACTT TGTAGAAAGA 
GCTCACACTG AACACGAGCT GCGGGGGCTT AGTCCTGGCC GCTCTTATAA CATCACAATA 
GTAACAGTAA AGCGCAACTT GCGGCACAAT GATATCAGTC GCCCCCTCTA CCTCACAGCA 
CGAACACGGC CACCTAACGT TGACAGGCCG AGGATTAATT CTGTCATGGA CACATCTGTC 
TCAGTTTCCT GGTCACTAAG TCCTCACAGA CACACATCTA TTGCTCGTGT TCGTCTGTTT 
CTCCAGCACC CAACGGAGCC GCCCCAGCAG GCAGAGCAGG AGCCATCAGC AGGAGAATAT 
ATCTTCAGGG ATCTACGTCC TGGAGAAAAA TACAGCGTCC AAATTAGCAC CTTGAGCCGC 
TCTGACCCCA GCCACCCAGC CACAGAAAGC CTGCCCAGTG CACCTTTGAT AGCCTGGACA 
CGTCCACACC CACCTTCCAA CCTTACGGCA GCTCTTGTCA CAGCCAATTC TGTGCATCTA 
ATTTGGGAGC AACCTCCTCA TGGGTCAGTG GATGGCTATA TCTTCAATGT GACCAATAAT 
CACAGTACAA AGAGCCGGTA TGTTCCCAGT GGAAAGATGA CTTCATATAC AGTCCGTGAC 
TTGCAGGCCG GCCAACGCTA CCGGATAACA GTGACTGCAC TACGCAACAC TCCCCAGGGC 
CCAGTGAACA GTGAGCCCAA GACAGTGCGT CCACAGACAC TGCAAAGGGA GGGACCTCAG 
GAGCGTCGTT GGACCCCCCC TAGAATTCTG CGGAACAGGC CTCCTCCACA GCCACATCAG 
GAGGTGCGAC TGCTGACAGA AAGGGGTCCT CCTCCTGCTG ATACTCCACA GCCGCACCGA 
TTCACAGAGC TGAACGATGG GCGCCGTCGT ATTTCAGCAA GGTTCAGCCA TCGTACCAAT 
AAAGCTATTA ATGTTACAAA AGAGCCAGAC CCTCCAGTCA AAATTGAGAG CTCAGACGAA 
GCTGCTATTC CTGTGAGGCA GGCGCTGCAG CTACAGGACC CTGAGCAAAG AAGCAAAGAA 
GGAGAACGAC TGGGCTGCCT GGAGACCCCG TGTCTTAATG GAGGGACCTG TGTGAATGGA 
GACACTTGTG ACTGTCCTCT GGGATTTAAA GGGCAGCAAT GCCAGTTGGC CTGCAGAAAG 
GAGACCTTTT CCTGCACAAG ACTGATTTCT GAGACAACAT CACACCCAGT GTGGGAGGGA 
GGAATCTGCC ACCACCTGTA CAGAAGAGTT TACAAGGTAC AGAATGATGT GTGCAGCAGG 
GAGGTTTGTG AAGGTACCAG CAGAAGCAGA GCAAGTGAGT GGACTCTTTT GGTTACAATT 
ACAGAAAGCA GAATCCCTTC CAAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sned1 ( XM_012970835.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826289.1 Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X8, mRNA.

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CloneID	OXa37193
Clone ID Related Accession (Same CDS sequence)	XM_012970835.2 , XM_012970835.1
Accession Version	XM_012970835.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sushi, nidogen and EGF-like domain-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATGGA TCTGTTGCAC ATTCACTAAC ATGGGGAAAT GCTACTCCCA GCCCTGTCAG 
AATGACGGAA CCTGTGTGGA TGAGCCTGGA AGCTTCACCT GTCTGTGTAC CTCAGATTTT 
ACAGGAACCT TATGTGAGAC AGAGGTATCG CCCTGCACAT ATATGACCTG TCATAATGGT 
GGGGAGTGCG AGGATCATAA TGGAATTGCA GTATGCGTGT GTCAGCGAGG ATACACAGGT 
GACAACTGTG ATACAGAACT CTTGCCATGC TCTTCCTCTC CTTGTTTGAA TGGTGGGTCC 
TGCGAGAATA TTGATGCTGG GTTTCGCTGT CAGTGCGCTT TGGGATTTAC TGGAACACAC 
TGTGAAAAAG AACCCAGCGC ATGCTTTTCG TCTCCCTGTA TGAATAACGG AGTCTGCAAG 
GATGTGGGTG CTGGATACAT CTGTGTGTGT GTTCAGGGAT TCACTGGAAT GTATTGTGAA 
ACAGAGATCA GGCCATGTTC TTCAGCTCCA TGTTTAAACA GAGGTGTGTG CAGAGAGAAT 
GGCGAGACTT ACAGCTGTCA GTGTGCACGC CGATTCACCG GAAAGCATTG CGAGACAGAG 
ATCAGACCTT GTTTTTCTAA TCCCTGTATG AACATGGGGA CATGTGAGGA CATGAGTGAA 
GGTTATAGCT GTATCTGCAC AGAAGGTTAC ACTGGAATCC ACTGTGAGAC AGAGTTAAGA 
CCCTGCTATT CATCCCCTTG CCTTAATGGG GCATTGTGCA AGGACTTTGG CATTACTTAC 
AGCTGTGTGT GTGCATGGGG GTTCACTGGT GCACGATGTG AGACAGAGCT GCACCCATGC 
TTTTCTTCTC CTTGCCTGAA TGGAGCAACA TGCAGAGATG TCGGTTTTGC TTACAGCTGT 
CAGTGTGCAC AGGGATTCAC AGGGATGCAC TGTGAGACAG AGATCAAGCC ATGTTTTTCA 
TCCCCTTGTC TGAACGGAGG GACCTGTGAG GACCTGGCAA TCAGCTTTGC CTGTTTATGT 
GCAGAGAGAT ATACTGGCTT GCACTGCGAG ACAGAGATAC AGCCTTGCTT TTCCTCTCCA 
TGCCTGAATA GAGGAATCTG TAATAATGAA GGTGCATTTT ACAGCTGTAC ATGTGCAGAG 
GGCTTTACAG GAGAAAACTG TGAAAAAGAG GTGAGCCCTT GCCTTTCTTC TCCCTGTCTG 
AATGGAGCAA CCTGCATTGA CATGGGTGCA AGTTACAGCT GCATGTGCCC ACAGGCCTTC 
ACTGGGGCGC TCTGTGACAC GGAGATACCA AGCCCATGTA CATCAAATCC TTGTGAGAAC 
AAAGGTGTGT GCAGGGAGGC AGAAGGAGGC TACTTGTGTG ACTGCCCCGC AGGTTTCAGT 
GGCATGCACT GCGAGCACCG TGTGTCCGAT GACTGCCCCT GCAGGAATGG AGGGCGATGT 
GCAGATGGAA ACAGCACTTG TCACTGCCCG CCAGGACACT TTGGGCTTCT CTGTGAATAT 
GAGGTCACCT CGCTACCGTG CAGCATGGGT TCACAGTGTC CAGATGGCGG ATCCTGCATG 
GAATATGGTG GAAGTTACTT GTGTGTGTGT CACACAGATT ACATCCTTAG TAACCACTCA 
ATACCCTCAC CCTGTGACTC GGATCCCTGC CTGAATGGTG GAACATGTGA ATCTCTAGAT 
GATGCATATA CATGTGCCTG TCCCCGTGGC TTCAATGGAA GACTTTGTGA GAAAGTAAAG 
CCAGCTCTGT GCAGCCTATC ACCTTGCCGC AATGGGGGCA CCTGCAAGGA GTCACGAGAA 
GAATATTATT GCGTTTGTCC CTATCCATTC ACTGGAAAAC ACTGTGAGAC TGGTATGAGG 
GGCCCTTGTT CGTCAGGTCC GTGTAATAAT GGTGGTACAT GTTTCCATTA TCTTGGGAAA 
TACAAGTGCG ACTGCCCACC AAACTTCTCT GGGCGACACT GTGAGAAAGC AGAATTGGAC 
TGTGGAGCCC CAGAGCAGGT AAAATATGCG AAGATGATTG TGACATCTAC ACTGCCGGGG 
GGTCGAGCAG AATATCACTG TAATGAAGGC TATGTGCTCA GTACTCCAAA CAACACTAGT 
GTGTGCAGTG AGGAAGGGAC ATGGAGCTTG CCCCCAGAGT GTGAAGAAAT AGACGAGTGT 
GCATCTCATC CCTGCCATAA TGGGGCATCC TGCAAGGACC GGATTTCCCA CTTTATCTGT 
GAATGCCAGA AAGGGTACAG TGGAAAGTAT TGTGAGCAAG AAACTAATGA GTGTCTGTCA 
CAGCCATGTA AAAATGGGGG TACCTGTCAG GATTTGCCTG GGACCTTCAA ATGTATTTGT 
CCTGAGGGTT TCACTGGATC TTATTGTGAG ACAGAGGTGG ATGGATGTGA CTCTTCTCCG 
TGCCAGAATG GAGGCTTGTG TGAGAACCTG GCGGGTTCTT ATCTGTGCGT GTGTCCCCGC 
GGCTTTTTTG GCTATCACTG TCAGACAGTG AGTGACATGT GCCTGTTGAA CCCCTGCGGT 
AACCGTGGGG TATGTGTGTC CAGCGCAGAT GGATTGATGA GCTGCACCTG CAGAACGGGG 
TACACTGGAA CCATGTGTGA GAAAGAGCTG CTGCCGCCAT TGTCACTGTC TCTGGGTGCG 
GTTGGAGAAA ACAGTGTTTG GGTGTTCTGG ACCCCCCCAG AAGATGGCAC TGGGCATGTA 
CTGGATGGGT TTGCAGTCAG TTACACTGCC CCAGATGGCT TATCTAGAAC AGACTTTGTA 
GAAAGAGCTC ACACTGAACA CGAGCTGCGG GGGCTTAGTC CTGGCCGCTC TTATAACATC 
ACAATAGTAA CAGTAAAGCG CAACTTGCGG CACAATGATA TCAGTCGCCC CCTCTACCTC 
ACAGCACGAA CACGGCCACC TAACGTTGAC AGGCCGAGGA TTAATTCTGT CATGGACACA 
TCTGTCTCAG TTTCCTGGTC ACTAAGTCCT CACAGACACA CATCTATTGC TCGTGTTCGT 
CTGTTTCTCC AGCACCCAAC GGAGCCGCCC CAGCAGGCAG AGCAGGAGCC ATCAGCAGGA 
GAATATATCT TCAGGGATCT ACGTCCTGGA GAAAAATACA GCGTCCAAAT TAGCACCTTG 
AGCCGCTCTG ACCCCAGCCA CCCAGCCACA GAAAGCCTGC CCAGTGCACC TTTGATAGCC 
TGGACACGTC CACACCCACC TTCCAACCTT ACGGCAGCTC TTGTCACAGC CAATTCTGTG 
CATCTAATTT GGGAGCAACC TCCTCATGGG TCAGTGGATG GCTATATCTT CAATGTGACC 
AATAATCACA GTACAAAGAG CCGGTATGTT CCCAGTGGAA AGATGACTTC ATATACAGTC 
CGTGACTTGC AGGCCGGCCA ACGCTACCGG ATAACAGTGA CTGCACTACG CAACACTCCC 
CAGGGCCCAG TGAACAGTGA GCCCAAGACA GTGCGTCCAC AGACACTGCA AAGGGAGGGA 
CCTCAGGAGC GTCGTTGGAC CCCCCCTAGA ATTCTGCGGA ACAGGCCTCC TCCACAGCCA 
CATCAGGAGG TGCGACTGCT GACAGAAAGG GGTCCTCCTC CTGCTGATAC TCCACAGCCG 
CACCGATTCA CAGAGCTGAA CGATGGGCGC CGTCGTATTT CAGCAAGGTT CAGCCATCGT 
ACCAATAAAG CTATTAATGT TACAAAAGAG CCAGACCCTC CAGTCAAAAT TGAGAGCTCA 
GACGAAGCTG CTATTCCTGT GAGGCAGGCG CTGCAGCTAC AGGACCCTGA GCAAAGAAGC 
AAAGAAGGAG AACGACTGGG CTGCCTGGAG ACCCCGTGTC TTAATGGAGG GACCTGTGTG 
AATGGAGACA CTTGTGACTG TCCTCTGGGA TTTAAAGGGC AGCAATGCCA GTTGGCCTGC 
AGAAAGGAGA CCTTTTCCTG CACAAGACTG ATTTCTGAGA CAACATCACA CCCAGTGTGG 
GAGGGAGGAA TCTGCCACCA CCTGTACAGA AGAGTTTACA AGGTACAGAA TGATGTGTGC 
AGCAGGGAGG TTTGTGAAGG TACCAGCAGA AGCAGAGCAA GTGAGTGGAC TCTTTTGGTT 
ACAATTACAG AAAGCAGAAT CCCTTCCAAG CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826289.1
CDS	48..4163
Translation	MRWICCTFTNMGKCYSQPCQNDGTCVDEPGSFTCLCTSDFTGTLCETEVSPCTYMTCHNGGECEDHNGIAVCVCQRGYTGDNCDTELLPCSSSPCLNGGSCENIDAGFRCQCALGFTGTHCEKEPSACFSSPCMNNGVCKDVGAGYICVCVQGFTGMYCETEIRPCSSAPCLNRGVCRENGETYSCQCARRFTGKHCETEIRPCFSNPCMNMGTCEDMSEGYSCICTEGYTGIHCETELRPCYSSPCLNGALCKDFGITYSCVCAWGFTGARCETELHPCFSSPCLNGATCRDVGFAYSCQCAQGFTGMHCETEIKPCFSSPCLNGGTCEDLAISFACLCAERYTGLHCETEIQPCFSSPCLNRGICNNEGAFYSCTCAEGFTGENCEKEVSPCLSSPCLNGATCIDMGASYSCMCPQAFTGALCDTEIPSPCTSNPCENKGVCREAEGGYLCDCPAGFSGMHCEHRVSDDCPCRNGGRCADGNSTCHCPPGHFGLLCEYEVTSLPCSMGSQCPDGGSCMEYGGSYLCVCHTDYILSNHSIPSPCDSDPCLNGGTCESLDDAYTCACPRGFNGRLCEKVKPALCSLSPCRNGGTCKESREEYYCVCPYPFTGKHCETGMRGPCSSGPCNNGGTCFHYLGKYKCDCPPNFSGRHCEKAELDCGAPEQVKYAKMIVTSTLPGGRAEYHCNEGYVLSTPNNTSVCSEEGTWSLPPECEEIDECASHPCHNGASCKDRISHFICECQKGYSGKYCEQETNECLSQPCKNGGTCQDLPGTFKCICPEGFTGSYCETEVDGCDSSPCQNGGLCENLAGSYLCVCPRGFFGYHCQTVSDMCLLNPCGNRGVCVSSADGLMSCTCRTGYTGTMCEKELLPPLSLSLGAVGENSVWVFWTPPEDGTGHVLDGFAVSYTAPDGLSRTDFVERAHTEHELRGLSPGRSYNITIVTVKRNLRHNDISRPLYLTARTRPPNVDRPRINSVMDTSVSVSWSLSPHRHTSIARVRLFLQHPTEPPQQAEQEPSAGEYIFRDLRPGEKYSVQISTLSRSDPSHPATESLPSAPLIAWTRPHPPSNLTAALVTANSVHLIWEQPPHGSVDGYIFNVTNNHSTKSRYVPSGKMTSYTVRDLQAGQRYRITVTALRNTPQGPVNSEPKTVRPQTLQREGPQERRWTPPRILRNRPPPQPHQEVRLLTERGPPPADTPQPHRFTELNDGRRRISARFSHRTNKAINVTKEPDPPVKIESSDEAAIPVRQALQLQDPEQRSKEGERLGCLETPCLNGGTCVNGDTCDCPLGFKGQQCQLACRKETFSCTRLISETTSHPVWEGGICHHLYRRVYKVQNDVCSREVCEGTSRSRASEWTLLVTITESRIPSKP

Target ORF information:

RefSeq Version	XM_012970835.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970835.1

ORF Insert Sequence:

ATGAGATGGA TCTGTTGCAC ATTCACTAAC ATGGGGAAAT GCTACTCCCA GCCCTGTCAG 
AATGACGGAA CCTGTGTGGA TGAGCCTGGA AGCTTCACCT GTCTGTGTAC CTCAGATTTT 
ACAGGAACCT TATGTGAGAC AGAGGTATCG CCCTGCACAT ATATGACCTG TCATAATGGT 
GGGGAGTGCG AGGATCATAA TGGAATTGCA GTATGCGTGT GTCAGCGAGG ATACACAGGT 
GACAACTGTG ATACAGAACT CTTGCCATGC TCTTCCTCTC CTTGTTTGAA TGGTGGGTCC 
TGCGAGAATA TTGATGCTGG GTTTCGCTGT CAGTGCGCTT TGGGATTTAC TGGAACACAC 
TGTGAAAAAG AACCCAGCGC ATGCTTTTCG TCTCCCTGTA TGAATAACGG AGTCTGCAAG 
GATGTGGGTG CTGGATACAT CTGTGTGTGT GTTCAGGGAT TCACTGGAAT GTATTGTGAA 
ACAGAGATCA GGCCATGTTC TTCAGCTCCA TGTTTAAACA GAGGTGTGTG CAGAGAGAAT 
GGCGAGACTT ACAGCTGTCA GTGTGCACGC CGATTCACCG GAAAGCATTG CGAGACAGAG 
ATCAGACCTT GTTTTTCTAA TCCCTGTATG AACATGGGGA CATGTGAGGA CATGAGTGAA 
GGTTATAGCT GTATCTGCAC AGAAGGTTAC ACTGGAATCC ACTGTGAGAC AGAGTTAAGA 
CCCTGCTATT CATCCCCTTG CCTTAATGGG GCATTGTGCA AGGACTTTGG CATTACTTAC 
AGCTGTGTGT GTGCATGGGG GTTCACTGGT GCACGATGTG AGACAGAGCT GCACCCATGC 
TTTTCTTCTC CTTGCCTGAA TGGAGCAACA TGCAGAGATG TCGGTTTTGC TTACAGCTGT 
CAGTGTGCAC AGGGATTCAC AGGGATGCAC TGTGAGACAG AGATCAAGCC ATGTTTTTCA 
TCCCCTTGTC TGAACGGAGG GACCTGTGAG GACCTGGCAA TCAGCTTTGC CTGTTTATGT 
GCAGAGAGAT ATACTGGCTT GCACTGCGAG ACAGAGATAC AGCCTTGCTT TTCCTCTCCA 
TGCCTGAATA GAGGAATCTG TAATAATGAA GGTGCATTTT ACAGCTGTAC ATGTGCAGAG 
GGCTTTACAG GAGAAAACTG TGAAAAAGAG GTGAGCCCTT GCCTTTCTTC TCCCTGTCTG 
AATGGAGCAA CCTGCATTGA CATGGGTGCA AGTTACAGCT GCATGTGCCC ACAGGCCTTC 
ACTGGGGCGC TCTGTGACAC GGAGATACCA AGCCCATGTA CATCAAATCC TTGTGAGAAC 
AAAGGTGTGT GCAGGGAGGC AGAAGGAGGC TACTTGTGTG ACTGCCCCGC AGGTTTCAGT 
GGCATGCACT GCGAGCACCG TGTGTCCGAT GACTGCCCCT GCAGGAATGG AGGGCGATGT 
GCAGATGGAA ACAGCACTTG TCACTGCCCG CCAGGACACT TTGGGCTTCT CTGTGAATAT 
GAGGTCACCT CGCTACCGTG CAGCATGGGT TCACAGTGTC CAGATGGCGG ATCCTGCATG 
GAATATGGTG GAAGTTACTT GTGTGTGTGT CACACAGATT ACATCCTTAG TAACCACTCA 
ATACCCTCAC CCTGTGACTC GGATCCCTGC CTGAATGGTG GAACATGTGA ATCTCTAGAT 
GATGCATATA CATGTGCCTG TCCCCGTGGC TTCAATGGAA GACTTTGTGA GAAAGTAAAG 
CCAGCTCTGT GCAGCCTATC ACCTTGCCGC AATGGGGGCA CCTGCAAGGA GTCACGAGAA 
GAATATTATT GCGTTTGTCC CTATCCATTC ACTGGAAAAC ACTGTGAGAC TGGTATGAGG 
GGCCCTTGTT CGTCAGGTCC GTGTAATAAT GGTGGTACAT GTTTCCATTA TCTTGGGAAA 
TACAAGTGCG ACTGCCCACC AAACTTCTCT GGGCGACACT GTGAGAAAGC AGAATTGGAC 
TGTGGAGCCC CAGAGCAGGT AAAATATGCG AAGATGATTG TGACATCTAC ACTGCCGGGG 
GGTCGAGCAG AATATCACTG TAATGAAGGC TATGTGCTCA GTACTCCAAA CAACACTAGT 
GTGTGCAGTG AGGAAGGGAC ATGGAGCTTG CCCCCAGAGT GTGAAGAAAT AGACGAGTGT 
GCATCTCATC CCTGCCATAA TGGGGCATCC TGCAAGGACC GGATTTCCCA CTTTATCTGT 
GAATGCCAGA AAGGGTACAG TGGAAAGTAT TGTGAGCAAG AAACTAATGA GTGTCTGTCA 
CAGCCATGTA AAAATGGGGG TACCTGTCAG GATTTGCCTG GGACCTTCAA ATGTATTTGT 
CCTGAGGGTT TCACTGGATC TTATTGTGAG ACAGAGGTGG ATGGATGTGA CTCTTCTCCG 
TGCCAGAATG GAGGCTTGTG TGAGAACCTG GCGGGTTCTT ATCTGTGCGT GTGTCCCCGC 
GGCTTTTTTG GCTATCACTG TCAGACAGTG AGTGACATGT GCCTGTTGAA CCCCTGCGGT 
AACCGTGGGG TATGTGTGTC CAGCGCAGAT GGATTGATGA GCTGCACCTG CAGAACGGGG 
TACACTGGAA CCATGTGTGA GAAAGAGCTG CTGCCGCCAT TGTCACTGTC TCTGGGTGCG 
GTTGGAGAAA ACAGTGTTTG GGTGTTCTGG ACCCCCCCAG AAGATGGCAC TGGGCATGTA 
CTGGATGGGT TTGCAGTCAG TTACACTGCC CCAGATGGCT TATCTAGAAC AGACTTTGTA 
GAAAGAGCTC ACACTGAACA CGAGCTGCGG GGGCTTAGTC CTGGCCGCTC TTATAACATC 
ACAATAGTAA CAGTAAAGCG CAACTTGCGG CACAATGATA TCAGTCGCCC CCTCTACCTC 
ACAGCACGAA CACGGCCACC TAACGTTGAC AGGCCGAGGA TTAATTCTGT CATGGACACA 
TCTGTCTCAG TTTCCTGGTC ACTAAGTCCT CACAGACACA CATCTATTGC TCGTGTTCGT 
CTGTTTCTCC AGCACCCAAC GGAGCCGCCC CAGCAGGCAG AGCAGGAGCC ATCAGCAGGA 
GAATATATCT TCAGGGATCT ACGTCCTGGA GAAAAATACA GCGTCCAAAT TAGCACCTTG 
AGCCGCTCTG ACCCCAGCCA CCCAGCCACA GAAAGCCTGC CCAGTGCACC TTTGATAGCC 
TGGACACGTC CACACCCACC TTCCAACCTT ACGGCAGCTC TTGTCACAGC CAATTCTGTG 
CATCTAATTT GGGAGCAACC TCCTCATGGG TCAGTGGATG GCTATATCTT CAATGTGACC 
AATAATCACA GTACAAAGAG CCGGTATGTT CCCAGTGGAA AGATGACTTC ATATACAGTC 
CGTGACTTGC AGGCCGGCCA ACGCTACCGG ATAACAGTGA CTGCACTACG CAACACTCCC 
CAGGGCCCAG TGAACAGTGA GCCCAAGACA GTGCGTCCAC AGACACTGCA AAGGGAGGGA 
CCTCAGGAGC GTCGTTGGAC CCCCCCTAGA ATTCTGCGGA ACAGGCCTCC TCCACAGCCA 
CATCAGGAGG TGCGACTGCT GACAGAAAGG GGTCCTCCTC CTGCTGATAC TCCACAGCCG 
CACCGATTCA CAGAGCTGAA CGATGGGCGC CGTCGTATTT CAGCAAGGTT CAGCCATCGT 
ACCAATAAAG CTATTAATGT TACAAAAGAG CCAGACCCTC CAGTCAAAAT TGAGAGCTCA 
GACGAAGCTG CTATTCCTGT GAGGCAGGCG CTGCAGCTAC AGGACCCTGA GCAAAGAAGC 
AAAGAAGGAG AACGACTGGG CTGCCTGGAG ACCCCGTGTC TTAATGGAGG GACCTGTGTG 
AATGGAGACA CTTGTGACTG TCCTCTGGGA TTTAAAGGGC AGCAATGCCA GTTGGCCTGC 
AGAAAGGAGA CCTTTTCCTG CACAAGACTG ATTTCTGAGA CAACATCACA CCCAGTGTGG 
GAGGGAGGAA TCTGCCACCA CCTGTACAGA AGAGTTTACA AGGTACAGAA TGATGTGTGC 
AGCAGGGAGG TTTGTGAAGG TACCAGCAGA AGCAGAGCAA GTGAGTGGAC TCTTTTGGTT 
ACAATTACAG AAAGCAGAAT CCCTTCCAAG CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sned1 ( XM_012970836.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826290.1 Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X7, mRNA.

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CloneID	OXa37194
Clone ID Related Accession (Same CDS sequence)	XM_012970836.1
Accession Version	XM_012970836.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sushi, nidogen and EGF-like domain-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAC CTGTGTGGAT GAGCCTGGAA GCTTCACCTG TCTGTGTACC TCAGATTTTA 
CAGGAACCTT ATGTGAGACA GGTATCGCCC TGCACATATA TGACCTGTCA TAATGGTGGG 
GAGTGCGAGG ATCATAATGG AATTGCAGTA TGCGTGTGTC AGCGAGGATA CACAGGTGAC 
AACTGTGATA CAGAACTCTT GCCATGCTCT TCCTCTCCTT GTTTGAATGG TGGGTCCTGC 
GAGAATATTG ATGCTGGGTT TCGCTGTCAG TGCGCTTTGG GATTTACTGG AACACACTGT 
GAAAAAGAAC CCAGCGCATG CTTTTCGTCT CCCTGTATGA ATAACGGAGT CTGCAAGGAT 
GTGGGTGCTG GATACATCTG TGTGTGTGTT CAGGGATTCA CTGGAATGTA TTGTGAAACA 
GAGATCAGGC CATGTTCTTC AGCTCCATGT TTAAACAGAG GTGTGTGCAG AGAGAATGGC 
GAGACTTACA GCTGTCAGTG TGCACGCCGA TTCACCGGAA AGCATTGCGA GACAGAGATC 
AGACCTTGTT TTTCTAATCC CTGTATGAAC ATGGGGACAT GTGAGGACAT GAGTGAAGGT 
TATAGCTGTA TCTGCACAGA AGGTTACACT GGAATCCACT GTGAGACAGA GTTAAGACCC 
TGCTATTCAT CCCCTTGCCT TAATGGGGCA TTGTGCAAGG ACTTTGGCAT TACTTACAGC 
TGTGTGTGTG CATGGGGGTT CACTGGTGCA CGATGTGAGA CAGAGCTGCA CCCATGCTTT 
TCTTCTCCTT GCCTGAATGG AGCAACATGC AGAGATGTCG GTTTTGCTTA CAGCTGTCAG 
TGTGCACAGG GATTCACAGG GATGCACTGT GAGACAGAGA TCAAGCCATG TTTTTCATCC 
CCTTGTCTGA ACGGAGGGAC CTGTGAGGAC CTGGCAATCA GCTTTGCCTG TTTATGTGCA 
GAGAGATATA CTGGCTTGCA CTGCGAGACA GAGATACAGC CTTGCTTTTC CTCTCCATGC 
CTGAATAGAG GAATCTGTAA TAATGAAGGT GCATTTTACA GCTGTACATG TGCAGAGGGC 
TTTACAGGAG AAAACTGTGA AAAAGAGGTG AGCCCTTGCC TTTCTTCTCC CTGTCTGAAT 
GGAGCAACCT GCATTGACAT GGGTGCAAGT TACAGCTGCA TGTGCCCACA GGCCTTCACT 
GGGGCGCTCT GTGACACGGA GATACCAAGC CCATGTACAT CAAATCCTTG TGAGAACAAA 
GGTGTGTGCA GGGAGGCAGA AGGAGGCTAC TTGTGTGACT GCCCCGCAGG TTTCAGTGGC 
ATGCACTGCG AGCACCGTGT GTCCGATGAC TGCCCCTGCA GGAATGGAGG GCGATGTGCA 
GATGGAAACA GCACTTGTCA CTGCCCGCCA GGACACTTTG GGCTTCTCTG TGAATATGAG 
GTCACCTCGC TACCGTGCAG CATGGGTTCA CAGTGTCCAG ATGGCGGATC CTGCATGGAA 
TATGGTGGAA GTTACTTGTG TGTGTGTCAC ACAGATTACA TCCTTAGTAA CCACTCAATA 
CCCTCACCCT GTGACTCGGA TCCCTGCCTG AATGGTGGAA CATGTGAATC TCTAGATGAT 
GCATATACAT GTGCCTGTCC CCGTGGCTTC AATGGAAGAC TTTGTGAGAA AGTAAAGCCA 
GCTCTGTGCA GCCTATCACC TTGCCGCAAT GGGGGCACCT GCAAGGAGTC ACGAGAAGAA 
TATTATTGCG TTTGTCCCTA TCCATTCACT GGAAAACACT GTGAGACTGG TATGAGGGGC 
CCTTGTTCGT CAGGTCCGTG TAATAATGGT GGTACATGTT TCCATTATCT TGGGAAATAC 
AAGTGCGACT GCCCACCAAA CTTCTCTGGG CGACACTGTG AGAAAGCAGA ATTGGACTGT 
GGAGCCCCAG AGCAGGTAAA ATATGCGAAG ATGATTGTGA CATCTACACT GCCGGGGGGT 
CGAGCAGAAT ATCACTGTAA TGAAGGCTAT GTGCTCAGTA CTCCAAACAA CACTAGTGTG 
TGCAGTGAGG AAGGGACATG GAGCTTGCCC CCAGAGTGTG AAGAAATAGA CGAGTGTGCA 
TCTCATCCCT GCCATAATGG GGCATCCTGC AAGGACCGGA TTTCCCACTT TATCTGTGAA 
TGCCAGAAAG GGTACAGTGG AAAGTATTGT GAGCAAGAAA CTAATGAGTG TCTGTCACAG 
CCATGTAAAA ATGGGGGTAC CTGTCAGGAT TTGCCTGGGA CCTTCAAATG TATTTGTCCT 
GAGGGTTTCA CTGGATCTTA TTGTGAGACA GAGGTGGATG GATGTGACTC TTCTCCGTGC 
CAGAATGGAG GCTTGTGTGA GAACCTGGCG GGTTCTTATC TGTGCGTGTG TCCCCGCGGC 
TTTTTTGGCT ATCACTGTCA GACAGTGAGT GACATGTGCC TGTTGAACCC CTGCGGTAAC 
CGTGGGGTAT GTGTGTCCAG CGCAGATGGA TTGATGAGCT GCACCTGCAG AACGGGGTAC 
ACTGGAACCA TGTGTGAGAA AGAGCTGCTG CCGCCATTGT CACTGTCTCT GGGTGCGGTT 
GGAGAAAACA GTGTTTGGGT GTTCTGGACC CCCCCAGAAG ATGGCACTGG GCATGTACTG 
GATGGGTTTG CAGTCAGTTA CACTGCCCCA GATGGCTTAT CTAGAACAGA CTTTGTAGAA 
AGAGCTCACA CTGAACACGA GCTGCGGGGG CTTAGTCCTG GCCGCTCTTA TAACATCACA 
ATAGTAACAG TAAAGCGCAA CTTGCGGCAC AATGATATCA GTCGCCCCCT CTACCTCACA 
GCACGAACAC GGCCACCTAA CGTTGACAGG CCGAGGATTA ATTCTGTCAT GGACACATCT 
GTCTCAGTTT CCTGGTCACT AAGTCCTCAC AGACACACAT CTATTGCTCG TGTTCGTCTG 
TTTCTCCAGC ACCCAACGGA GCCGCCCCAG CAGGCAGAGC AGGAGCCATC AGCAGGAGAA 
TATATCTTCA GGGATCTACG TCCTGGAGAA AAATACAGCG TCCAAATTAG CACCTTGAGC 
CGCTCTGACC CCAGCCACCC AGCCACAGAA AGCCTGCCCA GTGCACCTTT GATAGCCTGG 
ACACGTCCAC ACCCACCTTC CAACCTTACG GCAGCTCTTG TCACAGCCAA TTCTGTGCAT 
CTAATTTGGG AGCAACCTCC TCATGGGTCA GTGGATGGCT ATATCTTCAA TGTGACCAAT 
AATCACAGTA CAAAGAGCCG GTATGTTCCC AGTGGAAAGA TGACTTCATA TACAGTCCGT 
GACTTGCAGG CCGGCCAACG CTACCGGATA ACAGTGACTG CACTACGCAA CACTCCCCAG 
GGCCCAGTGA ACAGTGAGCC CAAGACAGTG CGTCCACAGA CACTGCAAAG GGAGGGACCT 
CAGGAGCGTC GTTGGACCCC CCCTAGAATT CTGCGGAACA GGCCTCCTCC ACAGCCACAT 
CAGGAGGTGC GACTGCTGAC AGAAAGGGGT CCTCCTCCTG CTGATACTCC ACAGCCGCAC 
CGATTCACAG AGCTGAACGA TGGGCGCCGT CGTATTTCAG CAAGGTTCAG CCATCGTACC 
AATAAAGCTA TTAATGTTAC AAAAGAGCCA GACCCTCCAG TCAAAATTGA GAGCTCAGAC 
GAAGCTGCTA TTCCTGTGAG GCAGGCGCTG CAGCTACAGG ACCCTGAGCA AAGAAGCAAA 
GAAGGAGAAC GACTGGGCTG CCTGGAGACC CCGTGTCTTA ATGGAGGGAC CTGTGTGAAT 
GGAGACACTT GTGACTGTCC TCTGGGATTT AAAGGGCAGC AATGCCAGTT GGCCTGCAGA 
AAGGAGACCT TTTCCTGCAC AAGACTGATT TCTGAGACAA CATCACACCC AGTGTGGGAG 
GGAGGAATCT GCCACCACCT GTACAGAAGA GTTTACAAGG TACAGAATGA TGTGTGCAGC 
AGGGAGGTTT GTGAAGGTAC CAGCAGAAGC AGAGCAAGTG AGTGGACTCT TTTGGTTACA 
ATTACAGAAA GCAGAATCCC TTCCAAGCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826290.1
CDS	124..4176
Translation	MTEPVWMSLEASPVCVPQILQEPYVRQVSPCTYMTCHNGGECEDHNGIAVCVCQRGYTGDNCDTELLPCSSSPCLNGGSCENIDAGFRCQCALGFTGTHCEKEPSACFSSPCMNNGVCKDVGAGYICVCVQGFTGMYCETEIRPCSSAPCLNRGVCRENGETYSCQCARRFTGKHCETEIRPCFSNPCMNMGTCEDMSEGYSCICTEGYTGIHCETELRPCYSSPCLNGALCKDFGITYSCVCAWGFTGARCETELHPCFSSPCLNGATCRDVGFAYSCQCAQGFTGMHCETEIKPCFSSPCLNGGTCEDLAISFACLCAERYTGLHCETEIQPCFSSPCLNRGICNNEGAFYSCTCAEGFTGENCEKEVSPCLSSPCLNGATCIDMGASYSCMCPQAFTGALCDTEIPSPCTSNPCENKGVCREAEGGYLCDCPAGFSGMHCEHRVSDDCPCRNGGRCADGNSTCHCPPGHFGLLCEYEVTSLPCSMGSQCPDGGSCMEYGGSYLCVCHTDYILSNHSIPSPCDSDPCLNGGTCESLDDAYTCACPRGFNGRLCEKVKPALCSLSPCRNGGTCKESREEYYCVCPYPFTGKHCETGMRGPCSSGPCNNGGTCFHYLGKYKCDCPPNFSGRHCEKAELDCGAPEQVKYAKMIVTSTLPGGRAEYHCNEGYVLSTPNNTSVCSEEGTWSLPPECEEIDECASHPCHNGASCKDRISHFICECQKGYSGKYCEQETNECLSQPCKNGGTCQDLPGTFKCICPEGFTGSYCETEVDGCDSSPCQNGGLCENLAGSYLCVCPRGFFGYHCQTVSDMCLLNPCGNRGVCVSSADGLMSCTCRTGYTGTMCEKELLPPLSLSLGAVGENSVWVFWTPPEDGTGHVLDGFAVSYTAPDGLSRTDFVERAHTEHELRGLSPGRSYNITIVTVKRNLRHNDISRPLYLTARTRPPNVDRPRINSVMDTSVSVSWSLSPHRHTSIARVRLFLQHPTEPPQQAEQEPSAGEYIFRDLRPGEKYSVQISTLSRSDPSHPATESLPSAPLIAWTRPHPPSNLTAALVTANSVHLIWEQPPHGSVDGYIFNVTNNHSTKSRYVPSGKMTSYTVRDLQAGQRYRITVTALRNTPQGPVNSEPKTVRPQTLQREGPQERRWTPPRILRNRPPPQPHQEVRLLTERGPPPADTPQPHRFTELNDGRRRISARFSHRTNKAINVTKEPDPPVKIESSDEAAIPVRQALQLQDPEQRSKEGERLGCLETPCLNGGTCVNGDTCDCPLGFKGQQCQLACRKETFSCTRLISETTSHPVWEGGICHHLYRRVYKVQNDVCSREVCEGTSRSRASEWTLLVTITESRIPSKP

Target ORF information:

RefSeq Version	XM_012970836.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970836.1

ORF Insert Sequence:

ATGACGGAAC CTGTGTGGAT GAGCCTGGAA GCTTCACCTG TCTGTGTACC TCAGATTTTA 
CAGGAACCTT ATGTGAGACA GGTATCGCCC TGCACATATA TGACCTGTCA TAATGGTGGG 
GAGTGCGAGG ATCATAATGG AATTGCAGTA TGCGTGTGTC AGCGAGGATA CACAGGTGAC 
AACTGTGATA CAGAACTCTT GCCATGCTCT TCCTCTCCTT GTTTGAATGG TGGGTCCTGC 
GAGAATATTG ATGCTGGGTT TCGCTGTCAG TGCGCTTTGG GATTTACTGG AACACACTGT 
GAAAAAGAAC CCAGCGCATG CTTTTCGTCT CCCTGTATGA ATAACGGAGT CTGCAAGGAT 
GTGGGTGCTG GATACATCTG TGTGTGTGTT CAGGGATTCA CTGGAATGTA TTGTGAAACA 
GAGATCAGGC CATGTTCTTC AGCTCCATGT TTAAACAGAG GTGTGTGCAG AGAGAATGGC 
GAGACTTACA GCTGTCAGTG TGCACGCCGA TTCACCGGAA AGCATTGCGA GACAGAGATC 
AGACCTTGTT TTTCTAATCC CTGTATGAAC ATGGGGACAT GTGAGGACAT GAGTGAAGGT 
TATAGCTGTA TCTGCACAGA AGGTTACACT GGAATCCACT GTGAGACAGA GTTAAGACCC 
TGCTATTCAT CCCCTTGCCT TAATGGGGCA TTGTGCAAGG ACTTTGGCAT TACTTACAGC 
TGTGTGTGTG CATGGGGGTT CACTGGTGCA CGATGTGAGA CAGAGCTGCA CCCATGCTTT 
TCTTCTCCTT GCCTGAATGG AGCAACATGC AGAGATGTCG GTTTTGCTTA CAGCTGTCAG 
TGTGCACAGG GATTCACAGG GATGCACTGT GAGACAGAGA TCAAGCCATG TTTTTCATCC 
CCTTGTCTGA ACGGAGGGAC CTGTGAGGAC CTGGCAATCA GCTTTGCCTG TTTATGTGCA 
GAGAGATATA CTGGCTTGCA CTGCGAGACA GAGATACAGC CTTGCTTTTC CTCTCCATGC 
CTGAATAGAG GAATCTGTAA TAATGAAGGT GCATTTTACA GCTGTACATG TGCAGAGGGC 
TTTACAGGAG AAAACTGTGA AAAAGAGGTG AGCCCTTGCC TTTCTTCTCC CTGTCTGAAT 
GGAGCAACCT GCATTGACAT GGGTGCAAGT TACAGCTGCA TGTGCCCACA GGCCTTCACT 
GGGGCGCTCT GTGACACGGA GATACCAAGC CCATGTACAT CAAATCCTTG TGAGAACAAA 
GGTGTGTGCA GGGAGGCAGA AGGAGGCTAC TTGTGTGACT GCCCCGCAGG TTTCAGTGGC 
ATGCACTGCG AGCACCGTGT GTCCGATGAC TGCCCCTGCA GGAATGGAGG GCGATGTGCA 
GATGGAAACA GCACTTGTCA CTGCCCGCCA GGACACTTTG GGCTTCTCTG TGAATATGAG 
GTCACCTCGC TACCGTGCAG CATGGGTTCA CAGTGTCCAG ATGGCGGATC CTGCATGGAA 
TATGGTGGAA GTTACTTGTG TGTGTGTCAC ACAGATTACA TCCTTAGTAA CCACTCAATA 
CCCTCACCCT GTGACTCGGA TCCCTGCCTG AATGGTGGAA CATGTGAATC TCTAGATGAT 
GCATATACAT GTGCCTGTCC CCGTGGCTTC AATGGAAGAC TTTGTGAGAA AGTAAAGCCA 
GCTCTGTGCA GCCTATCACC TTGCCGCAAT GGGGGCACCT GCAAGGAGTC ACGAGAAGAA 
TATTATTGCG TTTGTCCCTA TCCATTCACT GGAAAACACT GTGAGACTGG TATGAGGGGC 
CCTTGTTCGT CAGGTCCGTG TAATAATGGT GGTACATGTT TCCATTATCT TGGGAAATAC 
AAGTGCGACT GCCCACCAAA CTTCTCTGGG CGACACTGTG AGAAAGCAGA ATTGGACTGT 
GGAGCCCCAG AGCAGGTAAA ATATGCGAAG ATGATTGTGA CATCTACACT GCCGGGGGGT 
CGAGCAGAAT ATCACTGTAA TGAAGGCTAT GTGCTCAGTA CTCCAAACAA CACTAGTGTG 
TGCAGTGAGG AAGGGACATG GAGCTTGCCC CCAGAGTGTG AAGAAATAGA CGAGTGTGCA 
TCTCATCCCT GCCATAATGG GGCATCCTGC AAGGACCGGA TTTCCCACTT TATCTGTGAA 
TGCCAGAAAG GGTACAGTGG AAAGTATTGT GAGCAAGAAA CTAATGAGTG TCTGTCACAG 
CCATGTAAAA ATGGGGGTAC CTGTCAGGAT TTGCCTGGGA CCTTCAAATG TATTTGTCCT 
GAGGGTTTCA CTGGATCTTA TTGTGAGACA GAGGTGGATG GATGTGACTC TTCTCCGTGC 
CAGAATGGAG GCTTGTGTGA GAACCTGGCG GGTTCTTATC TGTGCGTGTG TCCCCGCGGC 
TTTTTTGGCT ATCACTGTCA GACAGTGAGT GACATGTGCC TGTTGAACCC CTGCGGTAAC 
CGTGGGGTAT GTGTGTCCAG CGCAGATGGA TTGATGAGCT GCACCTGCAG AACGGGGTAC 
ACTGGAACCA TGTGTGAGAA AGAGCTGCTG CCGCCATTGT CACTGTCTCT GGGTGCGGTT 
GGAGAAAACA GTGTTTGGGT GTTCTGGACC CCCCCAGAAG ATGGCACTGG GCATGTACTG 
GATGGGTTTG CAGTCAGTTA CACTGCCCCA GATGGCTTAT CTAGAACAGA CTTTGTAGAA 
AGAGCTCACA CTGAACACGA GCTGCGGGGG CTTAGTCCTG GCCGCTCTTA TAACATCACA 
ATAGTAACAG TAAAGCGCAA CTTGCGGCAC AATGATATCA GTCGCCCCCT CTACCTCACA 
GCACGAACAC GGCCACCTAA CGTTGACAGG CCGAGGATTA ATTCTGTCAT GGACACATCT 
GTCTCAGTTT CCTGGTCACT AAGTCCTCAC AGACACACAT CTATTGCTCG TGTTCGTCTG 
TTTCTCCAGC ACCCAACGGA GCCGCCCCAG CAGGCAGAGC AGGAGCCATC AGCAGGAGAA 
TATATCTTCA GGGATCTACG TCCTGGAGAA AAATACAGCG TCCAAATTAG CACCTTGAGC 
CGCTCTGACC CCAGCCACCC AGCCACAGAA AGCCTGCCCA GTGCACCTTT GATAGCCTGG 
ACACGTCCAC ACCCACCTTC CAACCTTACG GCAGCTCTTG TCACAGCCAA TTCTGTGCAT 
CTAATTTGGG AGCAACCTCC TCATGGGTCA GTGGATGGCT ATATCTTCAA TGTGACCAAT 
AATCACAGTA CAAAGAGCCG GTATGTTCCC AGTGGAAAGA TGACTTCATA TACAGTCCGT 
GACTTGCAGG CCGGCCAACG CTACCGGATA ACAGTGACTG CACTACGCAA CACTCCCCAG 
GGCCCAGTGA ACAGTGAGCC CAAGACAGTG CGTCCACAGA CACTGCAAAG GGAGGGACCT 
CAGGAGCGTC GTTGGACCCC CCCTAGAATT CTGCGGAACA GGCCTCCTCC ACAGCCACAT 
CAGGAGGTGC GACTGCTGAC AGAAAGGGGT CCTCCTCCTG CTGATACTCC ACAGCCGCAC 
CGATTCACAG AGCTGAACGA TGGGCGCCGT CGTATTTCAG CAAGGTTCAG CCATCGTACC 
AATAAAGCTA TTAATGTTAC AAAAGAGCCA GACCCTCCAG TCAAAATTGA GAGCTCAGAC 
GAAGCTGCTA TTCCTGTGAG GCAGGCGCTG CAGCTACAGG ACCCTGAGCA AAGAAGCAAA 
GAAGGAGAAC GACTGGGCTG CCTGGAGACC CCGTGTCTTA ATGGAGGGAC CTGTGTGAAT 
GGAGACACTT GTGACTGTCC TCTGGGATTT AAAGGGCAGC AATGCCAGTT GGCCTGCAGA 
AAGGAGACCT TTTCCTGCAC AAGACTGATT TCTGAGACAA CATCACACCC AGTGTGGGAG 
GGAGGAATCT GCCACCACCT GTACAGAAGA GTTTACAAGG TACAGAATGA TGTGTGCAGC 
AGGGAGGTTT GTGAAGGTAC CAGCAGAAGC AGAGCAAGTG AGTGGACTCT TTTGGTTACA 
ATTACAGAAA GCAGAATCCC TTCCAAGCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sned1 ( XM_018094448.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949937.1 Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa44795
Clone ID Related Accession (Same CDS sequence)	XM_018094448.1
Accession Version	XM_018094448.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sushi, nidogen and EGF-like domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA 
GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA 
TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG 
GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAGGTGG ATGGATGTGA CTCTTCTCCG 
TGCCAGAATG GAGGCTTGTG TGAGAACCTG GCGGGTTCTT ATCTGTGCGT GTGTCCCCGC 
GGCTTTTTTG GCTATCACTG TCAGACAGTG AGTGACATGT GCCTGTTGAA CCCCTGCGGT 
AACCGTGGGG TATGTGTGTC CAGCGCAGAT GGATTGATGA GCTGCACCTG CAGAACGGGG 
TACACTGGAA CCATGTGTGA GAAAGAGCTG CTGCCGCCAT TGTCACTGTC TCTGGGTGCG 
GTTGGAGAAA ACAGTGTTTG GGTGTTCTGG ACCCCCCCAG AAGATGGCAC TGGGCATGTA 
CTGGATGGGT TTGCAGTCAG TTACACTGCC CCAGATGGCT TATCTAGAAC AGACTTTGTA 
GAAAGAGCTC ACACTGAACA CGAGCTGCGG GGGCTTAGTC CTGGCCGCTC TTATAACATC 
ACAATAGTAA CAGTAAAGCG CAACTTGCGG CACAATGATA TCAGTCGCCC CCTCTACCTC 
ACAGCACGAA CACGGCCACC TAACGTTGAC AGGCCGAGGA TTAATTCTGT CATGGACACA 
TCTGTCTCAG TTTCCTGGTC ACTAAGTCCT CACAGACACA CATCTATTGC TCGTGTTCGT 
CTGTTTCTCC AGCACCCAAC GGAGCCGCCC CAGCAGGCAG AGCAGGAGCC ATCAGCAGGA 
GAATATATCT TCAGGGATCT ACGTCCTGGA GAAAAATACA GCGTCCAAAT TAGCACCTTG 
AGCCGCTCTG ACCCCAGCCA CCCAGCCACA GAAAGCCTGC CCAGTGCACC TTTGATAGCC 
TGGACACGTC CACACCCACC TTCCAACCTT ACGGCAGCTC TTGTCACAGC CAATTCTGTG 
CATCTAATTT GGGAGCAACC TCCTCATGGG TCAGTGGATG GCTATATCTT CAATGTGACC 
AATAATCACA GTACAAAGAG CCGGTATGTT CCCAGTGGAA AGATGACTTC ATATACAGTC 
CGTGACTTGC AGGCCGGCCA ACGCTACCGG ATAACAGTGA CTGCACTACG CAACACTCCC 
CAGGGCCCAG TGAACAGTGA GCCCAAGACA GTGCGTCCAC AGACACTGCA AAGGGAGGGA 
CCTCAGGAGC GTCGTTGGAC CCCCCCTAGA ATTCTGCGGA ACAGGCCTCC TCCACAGCCA 
CATCAGGAGG TGCGACTGCT GACAGAAAGG GGTCCTCCTC CTGCTGATAC TCCACAGCCG 
CACCGATTCA CAGAGCTGAA CGATGGGCGC CGTCGTATTT CAGCAAGGTT CAGCCATCGT 
ACCAATAAAG CTATTAATGT TACAAAAGAG CCAGACCCTC CAGTCAAAAT TGAGAGCTCA 
GACGAAGCTG CTATTCCTGT GAGGCAGGCG CTGCAGCTAC AGGACCCTGA GCAAAGAAGC 
AAAGAAGGAG AACGACTGGG CTGCCTGGAG ACCCCGTGTC TTAATGGAGG GACCTGTGTG 
AATGGAGACA CTTGTGACTG TCCTCTGGGA TTTAAAGGGC AGCAATGCCA GTTGGCCTGC 
AGAAAGGAGA CCTTTTCCTG CACAAGACTG ATTTCTGAGA CAACATCACA CCCAGTGTGG 
GAGGGAGGAA TCTGCCACCA CCTGTACAGA AGAGTTTACA AGGTACAGAA TGATGTGTGC 
AGCAGGGAGG TTTGTGAAGG TACCAGCAGA AGCAGAGCAA GTGAGTGGAC TCTTTTGGTT 
ACAATTACAG AAAGCAGAAT CCCTTCCAAG CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949937.1
CDS	3785..8560
Translation	MRLPSLFWIFISMVWRAQGMVPLEDFYPFGTKEGDTVTRRQDDGGSGLLPISKAFTFFGDKHSGLYVNNNGVVSFLREVSQFTPVAFPISGDRRVVAAFWADVDNRRGGNVFYRETEDLVIVDRATRDVRTYFPELPDFHSTWVFIATWHRVTFYGGDSTSPVNTFQIVLITDGLRSFTIFNYETIEWTTGRHASSGGDSSGRGGIAAQAGFNAGDGWRYFNIPGSRTDDIARIANTTNVGVPGRWVFRIDNNHVQVGECRNATSVCWTLRPCQNGGRCIDDCVTGNPSYTCSCLSGFTGKHCHIDMGKCYSQPCQNDGTCVDEPGSFTCLCTSDFTGTLCETEVSPCTYMTCHNGGECEDHNGIAVCVCQRGYTGDNCDTELLPCSSSPCLNGGSCENIDAGFRCQCALGFTGTHCEKEPSACFSSPCMNNGVCKDVGAGYICVCVQGFTGMYCETEIRPCSSAPCLNRGVCRENGETYSCQCARRFTGKHCETEIRPCFSNPCMNMGTCEDMSEGYSCICTEGYTGIHCETELRPCYSSPCLNGALCKDFGITYSCVCAWGFTGARCETELHPCFSSPCLNGATCRDVGFAYSCQCAQGFTGMHCETEIKPCFSSPCLNGGTCEDLAISFACLCAERYTGLHCETEIQPCFSSPCLNRGICNNEGAFYSCTCAEGFTGENCEKEVSPCLSSPCLNGATCIDMGASYSCMCPQAFTGALCDTEIPSPCTSNPCENKGVCREAEGGYLCDCPAGFSGMHCEHRVSDDCPCRNGGRCADGNSTCHCPPGHFGLLCEYEVTSLPCSMGSQCPDGGSCMEYGGSYLCVCHTDYILSNHSIPSPCDSDPCLNGGTCESLDDAYTCACPRGFNGRLCEKVKPALCSLSPCRNGGTCKESREEYYCVCPYPFTGKHCETGMRGPCSSGPCNNGGTCFHYLGKYKCDCPPNFSGRHCEKAELDCGAPEQVKYAKMIVTSTLPGGRAEYHCNEGYVLSTPNNTSVCSEEGTWSLPPECEEVDGCDSSPCQNGGLCENLAGSYLCVCPRGFFGYHCQTVSDMCLLNPCGNRGVCVSSADGLMSCTCRTGYTGTMCEKELLPPLSLSLGAVGENSVWVFWTPPEDGTGHVLDGFAVSYTAPDGLSRTDFVERAHTEHELRGLSPGRSYNITIVTVKRNLRHNDISRPLYLTARTRPPNVDRPRINSVMDTSVSVSWSLSPHRHTSIARVRLFLQHPTEPPQQAEQEPSAGEYIFRDLRPGEKYSVQISTLSRSDPSHPATESLPSAPLIAWTRPHPPSNLTAALVTANSVHLIWEQPPHGSVDGYIFNVTNNHSTKSRYVPSGKMTSYTVRDLQAGQRYRITVTALRNTPQGPVNSEPKTVRPQTLQREGPQERRWTPPRILRNRPPPQPHQEVRLLTERGPPPADTPQPHRFTELNDGRRRISARFSHRTNKAINVTKEPDPPVKIESSDEAAIPVRQALQLQDPEQRSKEGERLGCLETPCLNGGTCVNGDTCDCPLGFKGQQCQLACRKETFSCTRLISETTSHPVWEGGICHHLYRRVYKVQNDVCSREVCEGTSRSRASEWTLLVTITESRIPSKP

Target ORF information:

RefSeq Version	XM_018094448.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094448.1

ORF Insert Sequence:

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA 
GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA 
TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG 
GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAGGTGG ATGGATGTGA CTCTTCTCCG 
TGCCAGAATG GAGGCTTGTG TGAGAACCTG GCGGGTTCTT ATCTGTGCGT GTGTCCCCGC 
GGCTTTTTTG GCTATCACTG TCAGACAGTG AGTGACATGT GCCTGTTGAA CCCCTGCGGT 
AACCGTGGGG TATGTGTGTC CAGCGCAGAT GGATTGATGA GCTGCACCTG CAGAACGGGG 
TACACTGGAA CCATGTGTGA GAAAGAGCTG CTGCCGCCAT TGTCACTGTC TCTGGGTGCG 
GTTGGAGAAA ACAGTGTTTG GGTGTTCTGG ACCCCCCCAG AAGATGGCAC TGGGCATGTA 
CTGGATGGGT TTGCAGTCAG TTACACTGCC CCAGATGGCT TATCTAGAAC AGACTTTGTA 
GAAAGAGCTC ACACTGAACA CGAGCTGCGG GGGCTTAGTC CTGGCCGCTC TTATAACATC 
ACAATAGTAA CAGTAAAGCG CAACTTGCGG CACAATGATA TCAGTCGCCC CCTCTACCTC 
ACAGCACGAA CACGGCCACC TAACGTTGAC AGGCCGAGGA TTAATTCTGT CATGGACACA 
TCTGTCTCAG TTTCCTGGTC ACTAAGTCCT CACAGACACA CATCTATTGC TCGTGTTCGT 
CTGTTTCTCC AGCACCCAAC GGAGCCGCCC CAGCAGGCAG AGCAGGAGCC ATCAGCAGGA 
GAATATATCT TCAGGGATCT ACGTCCTGGA GAAAAATACA GCGTCCAAAT TAGCACCTTG 
AGCCGCTCTG ACCCCAGCCA CCCAGCCACA GAAAGCCTGC CCAGTGCACC TTTGATAGCC 
TGGACACGTC CACACCCACC TTCCAACCTT ACGGCAGCTC TTGTCACAGC CAATTCTGTG 
CATCTAATTT GGGAGCAACC TCCTCATGGG TCAGTGGATG GCTATATCTT CAATGTGACC 
AATAATCACA GTACAAAGAG CCGGTATGTT CCCAGTGGAA AGATGACTTC ATATACAGTC 
CGTGACTTGC AGGCCGGCCA ACGCTACCGG ATAACAGTGA CTGCACTACG CAACACTCCC 
CAGGGCCCAG TGAACAGTGA GCCCAAGACA GTGCGTCCAC AGACACTGCA AAGGGAGGGA 
CCTCAGGAGC GTCGTTGGAC CCCCCCTAGA ATTCTGCGGA ACAGGCCTCC TCCACAGCCA 
CATCAGGAGG TGCGACTGCT GACAGAAAGG GGTCCTCCTC CTGCTGATAC TCCACAGCCG 
CACCGATTCA CAGAGCTGAA CGATGGGCGC CGTCGTATTT CAGCAAGGTT CAGCCATCGT 
ACCAATAAAG CTATTAATGT TACAAAAGAG CCAGACCCTC CAGTCAAAAT TGAGAGCTCA 
GACGAAGCTG CTATTCCTGT GAGGCAGGCG CTGCAGCTAC AGGACCCTGA GCAAAGAAGC 
AAAGAAGGAG AACGACTGGG CTGCCTGGAG ACCCCGTGTC TTAATGGAGG GACCTGTGTG 
AATGGAGACA CTTGTGACTG TCCTCTGGGA TTTAAAGGGC AGCAATGCCA GTTGGCCTGC 
AGAAAGGAGA CCTTTTCCTG CACAAGACTG ATTTCTGAGA CAACATCACA CCCAGTGTGG 
GAGGGAGGAA TCTGCCACCA CCTGTACAGA AGAGTTTACA AGGTACAGAA TGATGTGTGC 
AGCAGGGAGG TTTGTGAAGG TACCAGCAGA AGCAGAGCAA GTGAGTGGAC TCTTTTGGTT 
ACAATTACAG AAAGCAGAAT CCCTTCCAAG CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sned1 ( XM_018094449.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949938.1 Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X7, mRNA.

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CloneID	OXa44796
Clone ID Related Accession (Same CDS sequence)	XM_018094449.1
Accession Version	XM_018094449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sushi, nidogen and EGF-like domain-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA 
GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA 
TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG 
GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC 
TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA 
GGGTACAGTG GAAAGTATTG TGAGCAAGAA ACTAATGAGT GTCTGTCACA GCCATGTAAA 
AATGGGGGTA CCTGTCAGGA TTTGCCTGGG ACCTTCAAAT GTATTTGTCC TGAGGGTTTC 
ACTGGATCTT ATTGTGAGAC AGAGGTGGAT GGATGTGACT CTTCTCCGTG CCAGAATGGA 
GGCTTGTGTG AGAACCTGGC GGGTTCTTAT CTGTGCGTGT GTCCCCGCGG CTTTTTTGGC 
TATCACTGTC AGACAGTGAG TGACATGTGC CTGTTGAACC CCTGCGGTAA CCGTGGGGTA 
TGTGTGTCCA GCGCAGATGG ATTGATGAGC TGCACCTGCA GAACGGGGTA CACTGGAACC 
ATGTGTGAGA AAGAGCTGCT GCCGCCATTG TCACTGTCTC TGGGTGCGGT TGGAGAAAAC 
AGTGTTTGGG TGTTCTGGAC CCCCCCAGAA GATGGCACTG GGCATGTACT GGATGGGTTT 
GCAGTCAGTT ACACTGCCCC AGATGGCTTA TCTAGAACAG ACTTTGTAGA AAGAGCTCAC 
ACTGAACACG AGCTGCGGGG GCTTAGTCCT GGCCGCTCTT ATAACATCAC AATAGTAACA 
GTAAAGCGCA ACTTGCGGCA CAATGATATC AGTCGCCCCC TCTACCTCAC AGCACGAACA 
CGGCCACCTA ACGTTGACAG GCCGAGGATT AATTCTGTCA TGGACACATC TGTCTCAGTT 
TCCTGGTCAC TAAGTCCTCA CAGACACACA TCTATTGCTC GTGTTCGTCT GTTTCTCCAG 
CACCCAACGG AGCCGCCCCA GCAGGCAGAG CAGGAGCCAT CAGCAGGAGA ATATATCTTC 
AGGGATCTAC GTCCTGGAGA AAAATACAGC GTCCAAATTA GCACCTTGAG CCGCTCTGAC 
CCCAGCCACC CAGCCACAGA AAGCCTGCCC AGTGCACCTT TGATAGCCTG GACACGTCCA 
CACCCACCTT CCAACCTTAC GGCAGCTCTT GTCACAGCCA ATTCTGTGCA TCTAATTTGG 
GAGCAACCTC CTCATGGGTC AGTGGATGGC TATATCTTCA ATGTGACCAA TAATCACAGT 
ACAAAGAGCC GGTATGTTCC CAGTGGAAAG ATGACTTCAT ATACAGTCCG TGACTTGCAG 
GCCGGCCAAC GCTACCGGAT AACAGTGACT GCACTACGCA ACACTCCCCA GGGCCCAGTG 
AACAGTGAGC CCAAGACAGT GCGTCCACAG ACACGTAATG CAAAGGGAGG GACCTCAGGA 
GCGTCGTTGG ACCCCCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949938.1
CDS	3785..8185
Translation	MRLPSLFWIFISMVWRAQGMVPLEDFYPFGTKEGDTVTRRQDDGGSGLLPISKAFTFFGDKHSGLYVNNNGVVSFLREVSQFTPVAFPISGDRRVVAAFWADVDNRRGGNVFYRETEDLVIVDRATRDVRTYFPELPDFHSTWVFIATWHRVTFYGGDSTSPVNTFQIVLITDGLRSFTIFNYETIEWTTGRHASSGGDSSGRGGIAAQAGFNAGDGWRYFNIPGSRTDDIARIANTTNVGVPGRWVFRIDNNHVQVGECRNATSVCWTLRPCQNGGRCIDDCVTGNPSYTCSCLSGFTGKHCHIDMGKCYSQPCQNDGTCVDEPGSFTCLCTSDFTGTLCETEVSPCTYMTCHNGGECEDHNGIAVCVCQRGYTGDNCDTELLPCSSSPCLNGGSCENIDAGFRCQCALGFTGTHCEKEPSACFSSPCMNNGVCKDVGAGYICVCVQGFTGMYCETEIRPCSSAPCLNRGVCRENGETYSCQCARRFTGKHCETEIRPCFSNPCMNMGTCEDMSEGYSCICTEGYTGIHCETELRPCYSSPCLNGALCKDFGITYSCVCAWGFTGARCETELHPCFSSPCLNGATCRDVGFAYSCQCAQGFTGMHCETEIKPCFSSPCLNGGTCEDLAISFACLCAERYTGLHCETEIQPCFSSPCLNRGICNNEGAFYSCTCAEGFTGENCEKEVSPCLSSPCLNGATCIDMGASYSCMCPQAFTGALCDTEIPSPCTSNPCENKGVCREAEGGYLCDCPAGFSGMHCEHRVSDDCPCRNGGRCADGNSTCHCPPGHFGLLCEYEVTSLPCSMGSQCPDGGSCMEYGGSYLCVCHTDYILSNHSIPSPCDSDPCLNGGTCESLDDAYTCACPRGFNGRLCEKVKPALCSLSPCRNGGTCKESREEYYCVCPYPFTGKHCETGMRGPCSSGPCNNGGTCFHYLGKYKCDCPPNFSGRHCEKAELDCGAPEQVKYAKMIVTSTLPGGRAEYHCNEGYVLSTPNNTSVCSEEGTWSLPPECEEIDECASHPCHNGASCKDRISHFICECQKGYSGKYCEQETNECLSQPCKNGGTCQDLPGTFKCICPEGFTGSYCETEVDGCDSSPCQNGGLCENLAGSYLCVCPRGFFGYHCQTVSDMCLLNPCGNRGVCVSSADGLMSCTCRTGYTGTMCEKELLPPLSLSLGAVGENSVWVFWTPPEDGTGHVLDGFAVSYTAPDGLSRTDFVERAHTEHELRGLSPGRSYNITIVTVKRNLRHNDISRPLYLTARTRPPNVDRPRINSVMDTSVSVSWSLSPHRHTSIARVRLFLQHPTEPPQQAEQEPSAGEYIFRDLRPGEKYSVQISTLSRSDPSHPATESLPSAPLIAWTRPHPPSNLTAALVTANSVHLIWEQPPHGSVDGYIFNVTNNHSTKSRYVPSGKMTSYTVRDLQAGQRYRITVTALRNTPQGPVNSEPKTVRPQTRNAKGGTSGASLDPP

Target ORF information:

RefSeq Version	XM_018094449.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094449.1

ORF Insert Sequence:

ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG 
CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC 
AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC 
CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG 
GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG 
ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC 
TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT 
TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT 
TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT 
TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC 
TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA 
GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC 
AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC 
GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA 
AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC 
TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA 
TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG 
GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT 
ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT 
GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA 
CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT 
GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG 
CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC 
AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT 
TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT 
ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA 
TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT 
GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT 
TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG 
GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG 
AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT 
ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA 
GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA 
GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC 
TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC 
TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC 
AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC 
GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC 
AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG 
CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA 
AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC 
TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA 
TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC 
AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC 
GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG 
TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC 
TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA 
GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA 
TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG 
GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC 
TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA 
GGGTACAGTG GAAAGTATTG TGAGCAAGAA ACTAATGAGT GTCTGTCACA GCCATGTAAA 
AATGGGGGTA CCTGTCAGGA TTTGCCTGGG ACCTTCAAAT GTATTTGTCC TGAGGGTTTC 
ACTGGATCTT ATTGTGAGAC AGAGGTGGAT GGATGTGACT CTTCTCCGTG CCAGAATGGA 
GGCTTGTGTG AGAACCTGGC GGGTTCTTAT CTGTGCGTGT GTCCCCGCGG CTTTTTTGGC 
TATCACTGTC AGACAGTGAG TGACATGTGC CTGTTGAACC CCTGCGGTAA CCGTGGGGTA 
TGTGTGTCCA GCGCAGATGG ATTGATGAGC TGCACCTGCA GAACGGGGTA CACTGGAACC 
ATGTGTGAGA AAGAGCTGCT GCCGCCATTG TCACTGTCTC TGGGTGCGGT TGGAGAAAAC 
AGTGTTTGGG TGTTCTGGAC CCCCCCAGAA GATGGCACTG GGCATGTACT GGATGGGTTT 
GCAGTCAGTT ACACTGCCCC AGATGGCTTA TCTAGAACAG ACTTTGTAGA AAGAGCTCAC 
ACTGAACACG AGCTGCGGGG GCTTAGTCCT GGCCGCTCTT ATAACATCAC AATAGTAACA 
GTAAAGCGCA ACTTGCGGCA CAATGATATC AGTCGCCCCC TCTACCTCAC AGCACGAACA 
CGGCCACCTA ACGTTGACAG GCCGAGGATT AATTCTGTCA TGGACACATC TGTCTCAGTT 
TCCTGGTCAC TAAGTCCTCA CAGACACACA TCTATTGCTC GTGTTCGTCT GTTTCTCCAG 
CACCCAACGG AGCCGCCCCA GCAGGCAGAG CAGGAGCCAT CAGCAGGAGA ATATATCTTC 
AGGGATCTAC GTCCTGGAGA AAAATACAGC GTCCAAATTA GCACCTTGAG CCGCTCTGAC 
CCCAGCCACC CAGCCACAGA AAGCCTGCCC AGTGCACCTT TGATAGCCTG GACACGTCCA 
CACCCACCTT CCAACCTTAC GGCAGCTCTT GTCACAGCCA ATTCTGTGCA TCTAATTTGG 
GAGCAACCTC CTCATGGGTC AGTGGATGGC TATATCTTCA ATGTGACCAA TAATCACAGT 
ACAAAGAGCC GGTATGTTCC CAGTGGAAAG ATGACTTCAT ATACAGTCCG TGACTTGCAG 
GCCGGCCAAC GCTACCGGAT AACAGTGACT GCACTACGCA ACACTCCCCA GGGCCCAGTG 
AACAGTGAGC CCAAGACAGT GCGTCCACAG ACACGTAATG CAAAGGGAGG GACCTCAGGA 
GCGTCGTTGG ACCCCCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	sned1
Entrez Gene ID	100498350
Full Name	sushi, nidogen and EGF-like domains 1
General protein information	Preferred Names sushi, nidogen and EGF-like domains 1 Names sushi, nidogen and EGF-like domain-containing protein 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Canis lupus familiaris (dog)	SNED1	XP_005636015.1
Gallus gallus (chicken)	SNED1	XP_003641796.1
Xenopus tropicalis (tropical clawed frog)	sned1	XP_002933383.2
Pan troglodytes (chimpanzee)	SNED1	XP_003309615.1
Mus musculus (house mouse)	Sned1	NP_766051.4
Danio rerio (zebrafish)	sned1	XP_001341690.4
Rattus norvegicus (Norway rat)	Sned1	NP_001161314.1
Homo sapiens (human)	SNED1	NP_001073906.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

sned1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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