sned1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following sned1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sned1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa37193 XM_012970835.2
Latest version!
Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa16046 XM_002933337.5
Latest version!
Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa37188 XM_012970829.3
Latest version!
Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa37189 XM_012970831.3
Latest version!
Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa37190 XM_031902549.1
Latest version!
Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa37191 XM_012970833.3
Latest version!
Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa37192 XM_012970834.2
Latest version!
Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OXa37194 XM_012970836.1
Latest version!
Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OXa44795 XM_018094448.1
Latest version!
Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OXa44796 XM_018094449.1
Latest version!
Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
View Old Accession Versions >>
OXa37188 XM_012970829.2 Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OXa37193 XM_012970835.1 Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OXa37191 XM_012970833.2 Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OXa37189 XM_012970831.2 Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OXa16046 XM_002933337.4 Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa37193
    Clone ID Related Accession (Same CDS sequence) XM_012970835.2 , XM_012970835.1
    Accession Version XM_012970835.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sushi, nidogen and EGF-like domain-containing protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970835.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGATGGA TCTGTTGCAC ATTCACTAAC ATGGGGAAAT GCTACTCCCA GCCCTGTCAG 
    AATGACGGAA CCTGTGTGGA TGAGCCTGGA AGCTTCACCT GTCTGTGTAC CTCAGATTTT
    ACAGGAACCT TATGTGAGAC AGAGGTATCG CCCTGCACAT ATATGACCTG TCATAATGGT
    GGGGAGTGCG AGGATCATAA TGGAATTGCA GTATGCGTGT GTCAGCGAGG ATACACAGGT
    GACAACTGTG ATACAGAACT CTTGCCATGC TCTTCCTCTC CTTGTTTGAA TGGTGGGTCC
    TGCGAGAATA TTGATGCTGG GTTTCGCTGT CAGTGCGCTT TGGGATTTAC TGGAACACAC
    TGTGAAAAAG AACCCAGCGC ATGCTTTTCG TCTCCCTGTA TGAATAACGG AGTCTGCAAG
    GATGTGGGTG CTGGATACAT CTGTGTGTGT GTTCAGGGAT TCACTGGAAT GTATTGTGAA
    ACAGAGATCA GGCCATGTTC TTCAGCTCCA TGTTTAAACA GAGGTGTGTG CAGAGAGAAT
    GGCGAGACTT ACAGCTGTCA GTGTGCACGC CGATTCACCG GAAAGCATTG CGAGACAGAG
    ATCAGACCTT GTTTTTCTAA TCCCTGTATG AACATGGGGA CATGTGAGGA CATGAGTGAA
    GGTTATAGCT GTATCTGCAC AGAAGGTTAC ACTGGAATCC ACTGTGAGAC AGAGTTAAGA
    CCCTGCTATT CATCCCCTTG CCTTAATGGG GCATTGTGCA AGGACTTTGG CATTACTTAC
    AGCTGTGTGT GTGCATGGGG GTTCACTGGT GCACGATGTG AGACAGAGCT GCACCCATGC
    TTTTCTTCTC CTTGCCTGAA TGGAGCAACA TGCAGAGATG TCGGTTTTGC TTACAGCTGT
    CAGTGTGCAC AGGGATTCAC AGGGATGCAC TGTGAGACAG AGATCAAGCC ATGTTTTTCA
    TCCCCTTGTC TGAACGGAGG GACCTGTGAG GACCTGGCAA TCAGCTTTGC CTGTTTATGT
    GCAGAGAGAT ATACTGGCTT GCACTGCGAG ACAGAGATAC AGCCTTGCTT TTCCTCTCCA
    TGCCTGAATA GAGGAATCTG TAATAATGAA GGTGCATTTT ACAGCTGTAC ATGTGCAGAG
    GGCTTTACAG GAGAAAACTG TGAAAAAGAG GTGAGCCCTT GCCTTTCTTC TCCCTGTCTG
    AATGGAGCAA CCTGCATTGA CATGGGTGCA AGTTACAGCT GCATGTGCCC ACAGGCCTTC
    ACTGGGGCGC TCTGTGACAC GGAGATACCA AGCCCATGTA CATCAAATCC TTGTGAGAAC
    AAAGGTGTGT GCAGGGAGGC AGAAGGAGGC TACTTGTGTG ACTGCCCCGC AGGTTTCAGT
    GGCATGCACT GCGAGCACCG TGTGTCCGAT GACTGCCCCT GCAGGAATGG AGGGCGATGT
    GCAGATGGAA ACAGCACTTG TCACTGCCCG CCAGGACACT TTGGGCTTCT CTGTGAATAT
    GAGGTCACCT CGCTACCGTG CAGCATGGGT TCACAGTGTC CAGATGGCGG ATCCTGCATG
    GAATATGGTG GAAGTTACTT GTGTGTGTGT CACACAGATT ACATCCTTAG TAACCACTCA
    ATACCCTCAC CCTGTGACTC GGATCCCTGC CTGAATGGTG GAACATGTGA ATCTCTAGAT
    GATGCATATA CATGTGCCTG TCCCCGTGGC TTCAATGGAA GACTTTGTGA GAAAGTAAAG
    CCAGCTCTGT GCAGCCTATC ACCTTGCCGC AATGGGGGCA CCTGCAAGGA GTCACGAGAA
    GAATATTATT GCGTTTGTCC CTATCCATTC ACTGGAAAAC ACTGTGAGAC TGGTATGAGG
    GGCCCTTGTT CGTCAGGTCC GTGTAATAAT GGTGGTACAT GTTTCCATTA TCTTGGGAAA
    TACAAGTGCG ACTGCCCACC AAACTTCTCT GGGCGACACT GTGAGAAAGC AGAATTGGAC
    TGTGGAGCCC CAGAGCAGGT AAAATATGCG AAGATGATTG TGACATCTAC ACTGCCGGGG
    GGTCGAGCAG AATATCACTG TAATGAAGGC TATGTGCTCA GTACTCCAAA CAACACTAGT
    GTGTGCAGTG AGGAAGGGAC ATGGAGCTTG CCCCCAGAGT GTGAAGAAAT AGACGAGTGT
    GCATCTCATC CCTGCCATAA TGGGGCATCC TGCAAGGACC GGATTTCCCA CTTTATCTGT
    GAATGCCAGA AAGGGTACAG TGGAAAGTAT TGTGAGCAAG AAACTAATGA GTGTCTGTCA
    CAGCCATGTA AAAATGGGGG TACCTGTCAG GATTTGCCTG GGACCTTCAA ATGTATTTGT
    CCTGAGGGTT TCACTGGATC TTATTGTGAG ACAGAGGTGG ATGGATGTGA CTCTTCTCCG
    TGCCAGAATG GAGGCTTGTG TGAGAACCTG GCGGGTTCTT ATCTGTGCGT GTGTCCCCGC
    GGCTTTTTTG GCTATCACTG TCAGACAGTG AGTGACATGT GCCTGTTGAA CCCCTGCGGT
    AACCGTGGGG TATGTGTGTC CAGCGCAGAT GGATTGATGA GCTGCACCTG CAGAACGGGG
    TACACTGGAA CCATGTGTGA GAAAGAGCTG CTGCCGCCAT TGTCACTGTC TCTGGGTGCG
    GTTGGAGAAA ACAGTGTTTG GGTGTTCTGG ACCCCCCCAG AAGATGGCAC TGGGCATGTA
    CTGGATGGGT TTGCAGTCAG TTACACTGCC CCAGATGGCT TATCTAGAAC AGACTTTGTA
    GAAAGAGCTC ACACTGAACA CGAGCTGCGG GGGCTTAGTC CTGGCCGCTC TTATAACATC
    ACAATAGTAA CAGTAAAGCG CAACTTGCGG CACAATGATA TCAGTCGCCC CCTCTACCTC
    ACAGCACGAA CACGGCCACC TAACGTTGAC AGGCCGAGGA TTAATTCTGT CATGGACACA
    TCTGTCTCAG TTTCCTGGTC ACTAAGTCCT CACAGACACA CATCTATTGC TCGTGTTCGT
    CTGTTTCTCC AGCACCCAAC GGAGCCGCCC CAGCAGGCAG AGCAGGAGCC ATCAGCAGGA
    GAATATATCT TCAGGGATCT ACGTCCTGGA GAAAAATACA GCGTCCAAAT TAGCACCTTG
    AGCCGCTCTG ACCCCAGCCA CCCAGCCACA GAAAGCCTGC CCAGTGCACC TTTGATAGCC
    TGGACACGTC CACACCCACC TTCCAACCTT ACGGCAGCTC TTGTCACAGC CAATTCTGTG
    CATCTAATTT GGGAGCAACC TCCTCATGGG TCAGTGGATG GCTATATCTT CAATGTGACC
    AATAATCACA GTACAAAGAG CCGGTATGTT CCCAGTGGAA AGATGACTTC ATATACAGTC
    CGTGACTTGC AGGCCGGCCA ACGCTACCGG ATAACAGTGA CTGCACTACG CAACACTCCC
    CAGGGCCCAG TGAACAGTGA GCCCAAGACA GTGCGTCCAC AGACACTGCA AAGGGAGGGA
    CCTCAGGAGC GTCGTTGGAC CCCCCCTAGA ATTCTGCGGA ACAGGCCTCC TCCACAGCCA
    CATCAGGAGG TGCGACTGCT GACAGAAAGG GGTCCTCCTC CTGCTGATAC TCCACAGCCG
    CACCGATTCA CAGAGCTGAA CGATGGGCGC CGTCGTATTT CAGCAAGGTT CAGCCATCGT
    ACCAATAAAG CTATTAATGT TACAAAAGAG CCAGACCCTC CAGTCAAAAT TGAGAGCTCA
    GACGAAGCTG CTATTCCTGT GAGGCAGGCG CTGCAGCTAC AGGACCCTGA GCAAAGAAGC
    AAAGAAGGAG AACGACTGGG CTGCCTGGAG ACCCCGTGTC TTAATGGAGG GACCTGTGTG
    AATGGAGACA CTTGTGACTG TCCTCTGGGA TTTAAAGGGC AGCAATGCCA GTTGGCCTGC
    AGAAAGGAGA CCTTTTCCTG CACAAGACTG ATTTCTGAGA CAACATCACA CCCAGTGTGG
    GAGGGAGGAA TCTGCCACCA CCTGTACAGA AGAGTTTACA AGGTACAGAA TGATGTGTGC
    AGCAGGGAGG TTTGTGAAGG TACCAGCAGA AGCAGAGCAA GTGAGTGGAC TCTTTTGGTT
    ACAATTACAG AAAGCAGAAT CCCTTCCAAG CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826289.1
    CDS36..4151
    Translation

    Target ORF information:

    RefSeq Version XM_012970835.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970835.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGATGGA TCTGTTGCAC ATTCACTAAC ATGGGGAAAT GCTACTCCCA GCCCTGTCAG 
    AATGACGGAA CCTGTGTGGA TGAGCCTGGA AGCTTCACCT GTCTGTGTAC CTCAGATTTT
    ACAGGAACCT TATGTGAGAC AGAGGTATCG CCCTGCACAT ATATGACCTG TCATAATGGT
    GGGGAGTGCG AGGATCATAA TGGAATTGCA GTATGCGTGT GTCAGCGAGG ATACACAGGT
    GACAACTGTG ATACAGAACT CTTGCCATGC TCTTCCTCTC CTTGTTTGAA TGGTGGGTCC
    TGCGAGAATA TTGATGCTGG GTTTCGCTGT CAGTGCGCTT TGGGATTTAC TGGAACACAC
    TGTGAAAAAG AACCCAGCGC ATGCTTTTCG TCTCCCTGTA TGAATAACGG AGTCTGCAAG
    GATGTGGGTG CTGGATACAT CTGTGTGTGT GTTCAGGGAT TCACTGGAAT GTATTGTGAA
    ACAGAGATCA GGCCATGTTC TTCAGCTCCA TGTTTAAACA GAGGTGTGTG CAGAGAGAAT
    GGCGAGACTT ACAGCTGTCA GTGTGCACGC CGATTCACCG GAAAGCATTG CGAGACAGAG
    ATCAGACCTT GTTTTTCTAA TCCCTGTATG AACATGGGGA CATGTGAGGA CATGAGTGAA
    GGTTATAGCT GTATCTGCAC AGAAGGTTAC ACTGGAATCC ACTGTGAGAC AGAGTTAAGA
    CCCTGCTATT CATCCCCTTG CCTTAATGGG GCATTGTGCA AGGACTTTGG CATTACTTAC
    AGCTGTGTGT GTGCATGGGG GTTCACTGGT GCACGATGTG AGACAGAGCT GCACCCATGC
    TTTTCTTCTC CTTGCCTGAA TGGAGCAACA TGCAGAGATG TCGGTTTTGC TTACAGCTGT
    CAGTGTGCAC AGGGATTCAC AGGGATGCAC TGTGAGACAG AGATCAAGCC ATGTTTTTCA
    TCCCCTTGTC TGAACGGAGG GACCTGTGAG GACCTGGCAA TCAGCTTTGC CTGTTTATGT
    GCAGAGAGAT ATACTGGCTT GCACTGCGAG ACAGAGATAC AGCCTTGCTT TTCCTCTCCA
    TGCCTGAATA GAGGAATCTG TAATAATGAA GGTGCATTTT ACAGCTGTAC ATGTGCAGAG
    GGCTTTACAG GAGAAAACTG TGAAAAAGAG GTGAGCCCTT GCCTTTCTTC TCCCTGTCTG
    AATGGAGCAA CCTGCATTGA CATGGGTGCA AGTTACAGCT GCATGTGCCC ACAGGCCTTC
    ACTGGGGCGC TCTGTGACAC GGAGATACCA AGCCCATGTA CATCAAATCC TTGTGAGAAC
    AAAGGTGTGT GCAGGGAGGC AGAAGGAGGC TACTTGTGTG ACTGCCCCGC AGGTTTCAGT
    GGCATGCACT GCGAGCACCG TGTGTCCGAT GACTGCCCCT GCAGGAATGG AGGGCGATGT
    GCAGATGGAA ACAGCACTTG TCACTGCCCG CCAGGACACT TTGGGCTTCT CTGTGAATAT
    GAGGTCACCT CGCTACCGTG CAGCATGGGT TCACAGTGTC CAGATGGCGG ATCCTGCATG
    GAATATGGTG GAAGTTACTT GTGTGTGTGT CACACAGATT ACATCCTTAG TAACCACTCA
    ATACCCTCAC CCTGTGACTC GGATCCCTGC CTGAATGGTG GAACATGTGA ATCTCTAGAT
    GATGCATATA CATGTGCCTG TCCCCGTGGC TTCAATGGAA GACTTTGTGA GAAAGTAAAG
    CCAGCTCTGT GCAGCCTATC ACCTTGCCGC AATGGGGGCA CCTGCAAGGA GTCACGAGAA
    GAATATTATT GCGTTTGTCC CTATCCATTC ACTGGAAAAC ACTGTGAGAC TGGTATGAGG
    GGCCCTTGTT CGTCAGGTCC GTGTAATAAT GGTGGTACAT GTTTCCATTA TCTTGGGAAA
    TACAAGTGCG ACTGCCCACC AAACTTCTCT GGGCGACACT GTGAGAAAGC AGAATTGGAC
    TGTGGAGCCC CAGAGCAGGT AAAATATGCG AAGATGATTG TGACATCTAC ACTGCCGGGG
    GGTCGAGCAG AATATCACTG TAATGAAGGC TATGTGCTCA GTACTCCAAA CAACACTAGT
    GTGTGCAGTG AGGAAGGGAC ATGGAGCTTG CCCCCAGAGT GTGAAGAAAT AGACGAGTGT
    GCATCTCATC CCTGCCATAA TGGGGCATCC TGCAAGGACC GGATTTCCCA CTTTATCTGT
    GAATGCCAGA AAGGGTACAG TGGAAAGTAT TGTGAGCAAG AAACTAATGA GTGTCTGTCA
    CAGCCATGTA AAAATGGGGG TACCTGTCAG GATTTGCCTG GGACCTTCAA ATGTATTTGT
    CCTGAGGGTT TCACTGGATC TTATTGTGAG ACAGAGGTGG ATGGATGTGA CTCTTCTCCG
    TGCCAGAATG GAGGCTTGTG TGAGAACCTG GCGGGTTCTT ATCTGTGCGT GTGTCCCCGC
    GGCTTTTTTG GCTATCACTG TCAGACAGTG AGTGACATGT GCCTGTTGAA CCCCTGCGGT
    AACCGTGGGG TATGTGTGTC CAGCGCAGAT GGATTGATGA GCTGCACCTG CAGAACGGGG
    TACACTGGAA CCATGTGTGA GAAAGAGCTG CTGCCGCCAT TGTCACTGTC TCTGGGTGCG
    GTTGGAGAAA ACAGTGTTTG GGTGTTCTGG ACCCCCCCAG AAGATGGCAC TGGGCATGTA
    CTGGATGGGT TTGCAGTCAG TTACACTGCC CCAGATGGCT TATCTAGAAC AGACTTTGTA
    GAAAGAGCTC ACACTGAACA CGAGCTGCGG GGGCTTAGTC CTGGCCGCTC TTATAACATC
    ACAATAGTAA CAGTAAAGCG CAACTTGCGG CACAATGATA TCAGTCGCCC CCTCTACCTC
    ACAGCACGAA CACGGCCACC TAACGTTGAC AGGCCGAGGA TTAATTCTGT CATGGACACA
    TCTGTCTCAG TTTCCTGGTC ACTAAGTCCT CACAGACACA CATCTATTGC TCGTGTTCGT
    CTGTTTCTCC AGCACCCAAC GGAGCCGCCC CAGCAGGCAG AGCAGGAGCC ATCAGCAGGA
    GAATATATCT TCAGGGATCT ACGTCCTGGA GAAAAATACA GCGTCCAAAT TAGCACCTTG
    AGCCGCTCTG ACCCCAGCCA CCCAGCCACA GAAAGCCTGC CCAGTGCACC TTTGATAGCC
    TGGACACGTC CACACCCACC TTCCAACCTT ACGGCAGCTC TTGTCACAGC CAATTCTGTG
    CATCTAATTT GGGAGCAACC TCCTCATGGG TCAGTGGATG GCTATATCTT CAATGTGACC
    AATAATCACA GTACAAAGAG CCGGTATGTT CCCAGTGGAA AGATGACTTC ATATACAGTC
    CGTGACTTGC AGGCCGGCCA ACGCTACCGG ATAACAGTGA CTGCACTACG CAACACTCCC
    CAGGGCCCAG TGAACAGTGA GCCCAAGACA GTGCGTCCAC AGACACTGCA AAGGGAGGGA
    CCTCAGGAGC GTCGTTGGAC CCCCCCTAGA ATTCTGCGGA ACAGGCCTCC TCCACAGCCA
    CATCAGGAGG TGCGACTGCT GACAGAAAGG GGTCCTCCTC CTGCTGATAC TCCACAGCCG
    CACCGATTCA CAGAGCTGAA CGATGGGCGC CGTCGTATTT CAGCAAGGTT CAGCCATCGT
    ACCAATAAAG CTATTAATGT TACAAAAGAG CCAGACCCTC CAGTCAAAAT TGAGAGCTCA
    GACGAAGCTG CTATTCCTGT GAGGCAGGCG CTGCAGCTAC AGGACCCTGA GCAAAGAAGC
    AAAGAAGGAG AACGACTGGG CTGCCTGGAG ACCCCGTGTC TTAATGGAGG GACCTGTGTG
    AATGGAGACA CTTGTGACTG TCCTCTGGGA TTTAAAGGGC AGCAATGCCA GTTGGCCTGC
    AGAAAGGAGA CCTTTTCCTG CACAAGACTG ATTTCTGAGA CAACATCACA CCCAGTGTGG
    GAGGGAGGAA TCTGCCACCA CCTGTACAGA AGAGTTTACA AGGTACAGAA TGATGTGTGC
    AGCAGGGAGG TTTGTGAAGG TACCAGCAGA AGCAGAGCAA GTGAGTGGAC TCTTTTGGTT
    ACAATTACAG AAAGCAGAAT CCCTTCCAAG CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa16046
    Clone ID Related Accession (Same CDS sequence) XM_002933337.5 , XM_002933337.4
    Accession Version XM_002933337.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4974bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sushi, nidogen and EGF-like domain-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002933337.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA
    GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA
    TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG
    GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC
    TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA
    GGGTACAGTG GAAAGTATTG TGAGCAAGAA ACTAATGAGT GTCTGTCACA GCCATGTAAA
    AATGGGGGTA CCTGTCAGGA TTTGCCTGGG ACCTTCAAAT GTATTTGTCC TGAGGGTTTC
    ACTGGATCTT ATTGTGAGAC AGAGGTGGAT GGATGTGACT CTTCTCCGTG CCAGAATGGA
    GGCTTGTGTG AGAACCTGGC GGGTTCTTAT CTGTGCGTGT GTCCCCGCGG CTTTTTTGGC
    TATCACTGTC AGACAGTGAG TGACATGTGC CTGTTGAACC CCTGCGGTAA CCGTGGGGTA
    TGTGTGTCCA GCGCAGATGG ATTGATGAGC TGCACCTGCA GAACGGGGTA CACTGGAACC
    ATGTGTGAGA AAGAGCTGCT GCCGCCATTG TCACTGTCTC TGGGTGCGGT TGGAGAAAAC
    AGTGTTTGGG TGTTCTGGAC CCCCCCAGAA GATGGCACTG GGCATGTACT GGATGGGTTT
    GCAGTCAGTT ACACTGCCCC AGATGGCTTA TCTAGAACAG ACTTTGTAGA AAGAGCTCAC
    ACTGAACACG AGCTGCGGGG GCTTAGTCCT GGCCGCTCTT ATAACATCAC AATAGTAACA
    GTAAAGCGCA ACTTGCGGCA CAATGATATC AGTCGCCCCC TCTACCTCAC AGCACGAACA
    CGGCCACCTA ACGTTGACAG GCCGAGGATT AATTCTGTCA TGGACACATC TGTCTCAGTT
    TCCTGGTCAC TAAGTCCTCA CAGACACACA TCTATTGCTC GTGTTCGTCT GTTTCTCCAG
    CACCCAACGG AGCCGCCCCA GCAGGCAGAG CAGGAGCCAT CAGCAGGAGA ATATATCTTC
    AGGGATCTAC GTCCTGGAGA AAAATACAGC GTCCAAATTA GCACCTTGAG CCGCTCTGAC
    CCCAGCCACC CAGCCACAGA AAGCCTGCCC AGTGCACCTT TGATAGCCTG GACACGTCCA
    CACCCACCTT CCAACCTTAC GGCAGCTCTT GTCACAGCCA ATTCTGTGCA TCTAATTTGG
    GAGCAACCTC CTCATGGGTC AGTGGATGGC TATATCTTCA ATGTGACCAA TAATCACAGT
    ACAAAGAGCC GGTATGTTCC CAGTGGAAAG ATGACTTCAT ATACAGTCCG TGACTTGCAG
    GCCGGCCAAC GCTACCGGAT AACAGTGACT GCACTACGCA ACACTCCCCA GGGCCCAGTG
    AACAGTGAGC CCAAGACAGT GCGTCCACAG ACACTGCAAA GGGAGGGACC TCAGGAGCGT
    CGTTGGACCC CCCCTAGAAT TCTGCGGAAC AGGCCTCCTC CACAGCCACA TCAGGAGGTG
    CGACTGCTGA CAGAAAGGGG TCCTCCTCCT GCTGATACTC CACAGCCGCA CCGATTCACA
    GAGCTGAACG ATGGGCGCCG TCGTATTTCA GCAAGGTTCA GCCATCGTAC CAATAAAGCT
    ATTAATGTTA CAAAAGAGCC AGACCCTCCA GTCAAAATTG AGAGCTCAGA CGAAGCTGCT
    ATTCCTGTGA GGCAGGCGCT GCAGCTACAG GACCCTGAGC AAAGAAGCAA AGAAGGAGAA
    CGACTGGGCT GCCTGGAGAC CCCGTGTCTT AATGGAGGGA CCTGTGTGAA TGGAGACACT
    TGTGACTGTC CTCTGGGATT TAAAGGGCAG CAATGCCAGT TGGCCTGCAG AAAGGAGACC
    TTTTCCTGCA CAAGACTGAT TTCTGAGACA ACATCACACC CAGTGTGGGA GGGAGGAATC
    TGCCACCACC TGTACAGAAG AGTTTACAAG GTACAGAATG ATGTGTGCAG CAGGGAGGTT
    TGTGAAGGTA CCAGCAGAAG CAGAGCAAAA AGCAGAATCC CTTCCAAGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002933383.2
    CDS1288..6261
    Translation

    Target ORF information:

    RefSeq Version XM_002933337.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002933337.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA
    GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA
    TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG
    GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC
    TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA
    GGGTACAGTG GAAAGTATTG TGAGCAAGAA ACTAATGAGT GTCTGTCACA GCCATGTAAA
    AATGGGGGTA CCTGTCAGGA TTTGCCTGGG ACCTTCAAAT GTATTTGTCC TGAGGGTTTC
    ACTGGATCTT ATTGTGAGAC AGAGGTGGAT GGATGTGACT CTTCTCCGTG CCAGAATGGA
    GGCTTGTGTG AGAACCTGGC GGGTTCTTAT CTGTGCGTGT GTCCCCGCGG CTTTTTTGGC
    TATCACTGTC AGACAGTGAG TGACATGTGC CTGTTGAACC CCTGCGGTAA CCGTGGGGTA
    TGTGTGTCCA GCGCAGATGG ATTGATGAGC TGCACCTGCA GAACGGGGTA CACTGGAACC
    ATGTGTGAGA AAGAGCTGCT GCCGCCATTG TCACTGTCTC TGGGTGCGGT TGGAGAAAAC
    AGTGTTTGGG TGTTCTGGAC CCCCCCAGAA GATGGCACTG GGCATGTACT GGATGGGTTT
    GCAGTCAGTT ACACTGCCCC AGATGGCTTA TCTAGAACAG ACTTTGTAGA AAGAGCTCAC
    ACTGAACACG AGCTGCGGGG GCTTAGTCCT GGCCGCTCTT ATAACATCAC AATAGTAACA
    GTAAAGCGCA ACTTGCGGCA CAATGATATC AGTCGCCCCC TCTACCTCAC AGCACGAACA
    CGGCCACCTA ACGTTGACAG GCCGAGGATT AATTCTGTCA TGGACACATC TGTCTCAGTT
    TCCTGGTCAC TAAGTCCTCA CAGACACACA TCTATTGCTC GTGTTCGTCT GTTTCTCCAG
    CACCCAACGG AGCCGCCCCA GCAGGCAGAG CAGGAGCCAT CAGCAGGAGA ATATATCTTC
    AGGGATCTAC GTCCTGGAGA AAAATACAGC GTCCAAATTA GCACCTTGAG CCGCTCTGAC
    CCCAGCCACC CAGCCACAGA AAGCCTGCCC AGTGCACCTT TGATAGCCTG GACACGTCCA
    CACCCACCTT CCAACCTTAC GGCAGCTCTT GTCACAGCCA ATTCTGTGCA TCTAATTTGG
    GAGCAACCTC CTCATGGGTC AGTGGATGGC TATATCTTCA ATGTGACCAA TAATCACAGT
    ACAAAGAGCC GGTATGTTCC CAGTGGAAAG ATGACTTCAT ATACAGTCCG TGACTTGCAG
    GCCGGCCAAC GCTACCGGAT AACAGTGACT GCACTACGCA ACACTCCCCA GGGCCCAGTG
    AACAGTGAGC CCAAGACAGT GCGTCCACAG ACACTGCAAA GGGAGGGACC TCAGGAGCGT
    CGTTGGACCC CCCCTAGAAT TCTGCGGAAC AGGCCTCCTC CACAGCCACA TCAGGAGGTG
    CGACTGCTGA CAGAAAGGGG TCCTCCTCCT GCTGATACTC CACAGCCGCA CCGATTCACA
    GAGCTGAACG ATGGGCGCCG TCGTATTTCA GCAAGGTTCA GCCATCGTAC CAATAAAGCT
    ATTAATGTTA CAAAAGAGCC AGACCCTCCA GTCAAAATTG AGAGCTCAGA CGAAGCTGCT
    ATTCCTGTGA GGCAGGCGCT GCAGCTACAG GACCCTGAGC AAAGAAGCAA AGAAGGAGAA
    CGACTGGGCT GCCTGGAGAC CCCGTGTCTT AATGGAGGGA CCTGTGTGAA TGGAGACACT
    TGTGACTGTC CTCTGGGATT TAAAGGGCAG CAATGCCAGT TGGCCTGCAG AAAGGAGACC
    TTTTCCTGCA CAAGACTGAT TTCTGAGACA ACATCACACC CAGTGTGGGA GGGAGGAATC
    TGCCACCACC TGTACAGAAG AGTTTACAAG GTACAGAATG ATGTGTGCAG CAGGGAGGTT
    TGTGAAGGTA CCAGCAGAAG CAGAGCAAAA AGCAGAATCC CTTCCAAGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37188
    Clone ID Related Accession (Same CDS sequence) XM_012970829.3 , XM_012970829.2
    Accession Version XM_012970829.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sushi, nidogen and EGF-like domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970829.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA
    GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA
    TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG
    GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC
    TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA
    GGGTACAGTG GAAAGTATTG TGAGCAAGAA ACTAATGAGT GTCTGTCACA GCCATGTAAA
    AATGGGGGTA CCTGTCAGGA TTTGCCTGGG ACCTTCAAAT GTATTTGTCC TGAGGGTTTC
    ACTGGATCTT ATTGTGAGAC AGAGGTGGAT GGATGTGACT CTTCTCCGTG CCAGAATGGA
    GGCTTGTGTG AGAACCTGGC GGGTTCTTAT CTGTGCGTGT GTCCCCGCGG CTTTTTTGGC
    TATCACTGTC AGACAGTGAG TGACATGTGC CTGTTGAACC CCTGCGGTAA CCGTGGGGTA
    TGTGTGTCCA GCGCAGATGG ATTGATGAGC TGCACCTGCA GAACGGGGTA CACTGGAACC
    ATGTGTGAGA AAGAGCTGCT GCCGCCATTG TCACTGTCTC TGGGTGCGGT TGGAGAAAAC
    AGTGTTTGGG TGTTCTGGAC CCCCCCAGAA GATGGCACTG GGCATGTACT GGATGGGTTT
    GCAGTCAGTT ACACTGCCCC AGATGGCTTA TCTAGAACAG ACTTTGTAGA AAGAGCTCAC
    ACTGAACACG AGCTGCGGGG GCTTAGTCCT GGCCGCTCTT ATAACATCAC AATAGTAACA
    GTAAAGCGCA ACTTGCGGCA CAATGATATC AGTCGCCCCC TCTACCTCAC AGCACGAACA
    CGGCCACCTA ACGTTGACAG GCCGAGGATT AATTCTGTCA TGGACACATC TGTCTCAGTT
    TCCTGGTCAC TAAGTCCTCA CAGACACACA TCTATTGCTC GTGTTCGTCT GTTTCTCCAG
    CACCCAACGG AGCCGCCCCA GCAGGCAGAG CAGGAGCCAT CAGCAGGAGA ATATATCTTC
    AGGGATCTAC GTCCTGGAGA AAAATACAGC GTCCAAATTA GCACCTTGAG CCGCTCTGAC
    CCCAGCCACC CAGCCACAGA AAGCCTGCCC AGTGCACCTT TGATAGCCTG GACACGTCCA
    CACCCACCTT CCAACCTTAC GGCAGCTCTT GTCACAGCCA ATTCTGTGCA TCTAATTTGG
    GAGCAACCTC CTCATGGGTC AGTGGATGGC TATATCTTCA ATGTGACCAA TAATCACAGT
    ACAAAGAGCC GGTATGTTCC CAGTGGAAAG ATGACTTCAT ATACAGTCCG TGACTTGCAG
    GCCGGCCAAC GCTACCGGAT AACAGTGACT GCACTACGCA ACACTCCCCA GGGCCCAGTG
    AACAGTGAGC CCAAGACAGT GCGTCCACAG ACACTGCAAA GGGAGGGACC TCAGGAGCGT
    CGTTGGACCC CCCCTAGAAT TCTGCGGAAC AGGCCTCCTC CACAGCCACA TCAGGAGGTG
    CGACTGCTGA CAGAAAGGGG TCCTCCTCCT GCTGATACTC CACAGCCGCA CCGATTCACA
    GAGCTGAACG ATGGGCGCCG TCGTATTTCA GCAAGGTTCA GCCATCGTAC CAATAAAGCT
    ATTAATGTTA CAAAAGAGCC AGACCCTCCA GTCAAAATTG AGAGCTCAGA CGAAGCTGCT
    ATTCCTGTGA GGCAGGCGCT GCAGCTACAG GACCCTGAGC AAAGAAGCAA AGAAGGAGAA
    CGACTGGGCT GCCTGGAGAC CCCGTGTCTT AATGGAGGGA CCTGTGTGAA TGGAGACACT
    TGTGACTGTC CTCTGGGATT TAAAGGGCAG CAATGCCAGT TGGCCTGCAG AAAGGAGACC
    TTTTCCTGCA CAAGACTGAT TTCTGAGACA ACATCACACC CAGTGTGGGA GGGAGGAATC
    TGCCACCACC TGTACAGAAG AGTTTACAAG GTACAGAATG ATGTGTGCAG CAGGGAGGTT
    TGTGAAGGTA CCAGCAGAAG CAGAGCAAGT GAGTGGACTC TTTTGGTTAC AATTACAGAA
    AGCAGAATCC CTTCCAAGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826283.1
    CDS1294..6297
    Translation

    Target ORF information:

    RefSeq Version XM_012970829.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970829.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA
    GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA
    TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG
    GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC
    TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA
    GGGTACAGTG GAAAGTATTG TGAGCAAGAA ACTAATGAGT GTCTGTCACA GCCATGTAAA
    AATGGGGGTA CCTGTCAGGA TTTGCCTGGG ACCTTCAAAT GTATTTGTCC TGAGGGTTTC
    ACTGGATCTT ATTGTGAGAC AGAGGTGGAT GGATGTGACT CTTCTCCGTG CCAGAATGGA
    GGCTTGTGTG AGAACCTGGC GGGTTCTTAT CTGTGCGTGT GTCCCCGCGG CTTTTTTGGC
    TATCACTGTC AGACAGTGAG TGACATGTGC CTGTTGAACC CCTGCGGTAA CCGTGGGGTA
    TGTGTGTCCA GCGCAGATGG ATTGATGAGC TGCACCTGCA GAACGGGGTA CACTGGAACC
    ATGTGTGAGA AAGAGCTGCT GCCGCCATTG TCACTGTCTC TGGGTGCGGT TGGAGAAAAC
    AGTGTTTGGG TGTTCTGGAC CCCCCCAGAA GATGGCACTG GGCATGTACT GGATGGGTTT
    GCAGTCAGTT ACACTGCCCC AGATGGCTTA TCTAGAACAG ACTTTGTAGA AAGAGCTCAC
    ACTGAACACG AGCTGCGGGG GCTTAGTCCT GGCCGCTCTT ATAACATCAC AATAGTAACA
    GTAAAGCGCA ACTTGCGGCA CAATGATATC AGTCGCCCCC TCTACCTCAC AGCACGAACA
    CGGCCACCTA ACGTTGACAG GCCGAGGATT AATTCTGTCA TGGACACATC TGTCTCAGTT
    TCCTGGTCAC TAAGTCCTCA CAGACACACA TCTATTGCTC GTGTTCGTCT GTTTCTCCAG
    CACCCAACGG AGCCGCCCCA GCAGGCAGAG CAGGAGCCAT CAGCAGGAGA ATATATCTTC
    AGGGATCTAC GTCCTGGAGA AAAATACAGC GTCCAAATTA GCACCTTGAG CCGCTCTGAC
    CCCAGCCACC CAGCCACAGA AAGCCTGCCC AGTGCACCTT TGATAGCCTG GACACGTCCA
    CACCCACCTT CCAACCTTAC GGCAGCTCTT GTCACAGCCA ATTCTGTGCA TCTAATTTGG
    GAGCAACCTC CTCATGGGTC AGTGGATGGC TATATCTTCA ATGTGACCAA TAATCACAGT
    ACAAAGAGCC GGTATGTTCC CAGTGGAAAG ATGACTTCAT ATACAGTCCG TGACTTGCAG
    GCCGGCCAAC GCTACCGGAT AACAGTGACT GCACTACGCA ACACTCCCCA GGGCCCAGTG
    AACAGTGAGC CCAAGACAGT GCGTCCACAG ACACTGCAAA GGGAGGGACC TCAGGAGCGT
    CGTTGGACCC CCCCTAGAAT TCTGCGGAAC AGGCCTCCTC CACAGCCACA TCAGGAGGTG
    CGACTGCTGA CAGAAAGGGG TCCTCCTCCT GCTGATACTC CACAGCCGCA CCGATTCACA
    GAGCTGAACG ATGGGCGCCG TCGTATTTCA GCAAGGTTCA GCCATCGTAC CAATAAAGCT
    ATTAATGTTA CAAAAGAGCC AGACCCTCCA GTCAAAATTG AGAGCTCAGA CGAAGCTGCT
    ATTCCTGTGA GGCAGGCGCT GCAGCTACAG GACCCTGAGC AAAGAAGCAA AGAAGGAGAA
    CGACTGGGCT GCCTGGAGAC CCCGTGTCTT AATGGAGGGA CCTGTGTGAA TGGAGACACT
    TGTGACTGTC CTCTGGGATT TAAAGGGCAG CAATGCCAGT TGGCCTGCAG AAAGGAGACC
    TTTTCCTGCA CAAGACTGAT TTCTGAGACA ACATCACACC CAGTGTGGGA GGGAGGAATC
    TGCCACCACC TGTACAGAAG AGTTTACAAG GTACAGAATG ATGTGTGCAG CAGGGAGGTT
    TGTGAAGGTA CCAGCAGAAG CAGAGCAAGT GAGTGGACTC TTTTGGTTAC AATTACAGAA
    AGCAGAATCC CTTCCAAGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37189
    Clone ID Related Accession (Same CDS sequence) XM_012970831.3 , XM_012970831.2
    Accession Version XM_012970831.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5001bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sushi, nidogen and EGF-like domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970831.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGAAT TGGACTGTGG AGCCCCAGAG
    CAGGTAAAAT ATGCGAAGAT GATTGTGACA TCTACACTGC CGGGGGGTCG AGCAGAATAT
    CACTGTAATG AAGGCTATGT GCTCAGTACT CCAAACAACA CTAGTGTGTG CAGTGAGGAA
    GGGACATGGA GCTTGCCCCC AGAGTGTGAA GAAATAGACG AGTGTGCATC TCATCCCTGC
    CATAATGGGG CATCCTGCAA GGACCGGATT TCCCACTTTA TCTGTGAATG CCAGAAAGGG
    TACAGTGGAA AGTATTGTGA GCAAGAAACT AATGAGTGTC TGTCACAGCC ATGTAAAAAT
    GGGGGTACCT GTCAGGATTT GCCTGGGACC TTCAAATGTA TTTGTCCTGA GGGTTTCACT
    GGATCTTATT GTGAGACAGA GGTGGATGGA TGTGACTCTT CTCCGTGCCA GAATGGAGGC
    TTGTGTGAGA ACCTGGCGGG TTCTTATCTG TGCGTGTGTC CCCGCGGCTT TTTTGGCTAT
    CACTGTCAGA CAGTGAGTGA CATGTGCCTG TTGAACCCCT GCGGTAACCG TGGGGTATGT
    GTGTCCAGCG CAGATGGATT GATGAGCTGC ACCTGCAGAA CGGGGTACAC TGGAACCATG
    TGTGAGAAAG AGCTGCTGCC GCCATTGTCA CTGTCTCTGG GTGCGGTTGG AGAAAACAGT
    GTTTGGGTGT TCTGGACCCC CCCAGAAGAT GGCACTGGGC ATGTACTGGA TGGGTTTGCA
    GTCAGTTACA CTGCCCCAGA TGGCTTATCT AGAACAGACT TTGTAGAAAG AGCTCACACT
    GAACACGAGC TGCGGGGGCT TAGTCCTGGC CGCTCTTATA ACATCACAAT AGTAACAGTA
    AAGCGCAACT TGCGGCACAA TGATATCAGT CGCCCCCTCT ACCTCACAGC ACGAACACGG
    CCACCTAACG TTGACAGGCC GAGGATTAAT TCTGTCATGG ACACATCTGT CTCAGTTTCC
    TGGTCACTAA GTCCTCACAG ACACACATCT ATTGCTCGTG TTCGTCTGTT TCTCCAGCAC
    CCAACGGAGC CGCCCCAGCA GGCAGAGCAG GAGCCATCAG CAGGAGAATA TATCTTCAGG
    GATCTACGTC CTGGAGAAAA ATACAGCGTC CAAATTAGCA CCTTGAGCCG CTCTGACCCC
    AGCCACCCAG CCACAGAAAG CCTGCCCAGT GCACCTTTGA TAGCCTGGAC ACGTCCACAC
    CCACCTTCCA ACCTTACGGC AGCTCTTGTC ACAGCCAATT CTGTGCATCT AATTTGGGAG
    CAACCTCCTC ATGGGTCAGT GGATGGCTAT ATCTTCAATG TGACCAATAA TCACAGTACA
    AAGAGCCGGT ATGTTCCCAG TGGAAAGATG ACTTCATATA CAGTCCGTGA CTTGCAGGCC
    GGCCAACGCT ACCGGATAAC AGTGACTGCA CTACGCAACA CTCCCCAGGG CCCAGTGAAC
    AGTGAGCCCA AGACAGTGCG TCCACAGACA CTGCAAAGGG AGGGACCTCA GGAGCGTCGT
    TGGACCCCCC CTAGAATTCT GCGGAACAGG CCTCCTCCAC AGCCACATCA GGAGGTGCGA
    CTGCTGACAG AAAGGGGTCC TCCTCCTGCT GATACTCCAC AGCCGCACCG ATTCACAGAG
    CTGAACGATG GGCGCCGTCG TATTTCAGCA AGGTTCAGCC ATCGTACCAA TAAAGCTATT
    AATGTTACAA AAGAGCCAGA CCCTCCAGTC AAAATTGAGA GCTCAGACGA AGCTGCTATT
    CCTGTGAGGC AGGCGCTGCA GCTACAGGAC CCTGAGCAAA GAAGCAAAGA AGGAGAACGA
    CTGGGCTGCC TGGAGACCCC GTGTCTTAAT GGAGGGACCT GTGTGAATGG AGACACTTGT
    GACTGTCCTC TGGGATTTAA AGGGCAGCAA TGCCAGTTGG CCTGCAGAAA GGAGACCTTT
    TCCTGCACAA GACTGATTTC TGAGACAACA TCACACCCAG TGTGGGAGGG AGGAATCTGC
    CACCACCTGT ACAGAAGAGT TTACAAGGTA CAGAATGATG TGTGCAGCAG GGAGGTTTGT
    GAAGGTACCA GCAGAAGCAG AGCAAGTGAG TGGACTCTTT TGGTTACAAT TACAGAAAGC
    AGAATCCCTT CCAAGCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826285.1
    CDS1296..6296
    Translation

    Target ORF information:

    RefSeq Version XM_012970831.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970831.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGAAT TGGACTGTGG AGCCCCAGAG
    CAGGTAAAAT ATGCGAAGAT GATTGTGACA TCTACACTGC CGGGGGGTCG AGCAGAATAT
    CACTGTAATG AAGGCTATGT GCTCAGTACT CCAAACAACA CTAGTGTGTG CAGTGAGGAA
    GGGACATGGA GCTTGCCCCC AGAGTGTGAA GAAATAGACG AGTGTGCATC TCATCCCTGC
    CATAATGGGG CATCCTGCAA GGACCGGATT TCCCACTTTA TCTGTGAATG CCAGAAAGGG
    TACAGTGGAA AGTATTGTGA GCAAGAAACT AATGAGTGTC TGTCACAGCC ATGTAAAAAT
    GGGGGTACCT GTCAGGATTT GCCTGGGACC TTCAAATGTA TTTGTCCTGA GGGTTTCACT
    GGATCTTATT GTGAGACAGA GGTGGATGGA TGTGACTCTT CTCCGTGCCA GAATGGAGGC
    TTGTGTGAGA ACCTGGCGGG TTCTTATCTG TGCGTGTGTC CCCGCGGCTT TTTTGGCTAT
    CACTGTCAGA CAGTGAGTGA CATGTGCCTG TTGAACCCCT GCGGTAACCG TGGGGTATGT
    GTGTCCAGCG CAGATGGATT GATGAGCTGC ACCTGCAGAA CGGGGTACAC TGGAACCATG
    TGTGAGAAAG AGCTGCTGCC GCCATTGTCA CTGTCTCTGG GTGCGGTTGG AGAAAACAGT
    GTTTGGGTGT TCTGGACCCC CCCAGAAGAT GGCACTGGGC ATGTACTGGA TGGGTTTGCA
    GTCAGTTACA CTGCCCCAGA TGGCTTATCT AGAACAGACT TTGTAGAAAG AGCTCACACT
    GAACACGAGC TGCGGGGGCT TAGTCCTGGC CGCTCTTATA ACATCACAAT AGTAACAGTA
    AAGCGCAACT TGCGGCACAA TGATATCAGT CGCCCCCTCT ACCTCACAGC ACGAACACGG
    CCACCTAACG TTGACAGGCC GAGGATTAAT TCTGTCATGG ACACATCTGT CTCAGTTTCC
    TGGTCACTAA GTCCTCACAG ACACACATCT ATTGCTCGTG TTCGTCTGTT TCTCCAGCAC
    CCAACGGAGC CGCCCCAGCA GGCAGAGCAG GAGCCATCAG CAGGAGAATA TATCTTCAGG
    GATCTACGTC CTGGAGAAAA ATACAGCGTC CAAATTAGCA CCTTGAGCCG CTCTGACCCC
    AGCCACCCAG CCACAGAAAG CCTGCCCAGT GCACCTTTGA TAGCCTGGAC ACGTCCACAC
    CCACCTTCCA ACCTTACGGC AGCTCTTGTC ACAGCCAATT CTGTGCATCT AATTTGGGAG
    CAACCTCCTC ATGGGTCAGT GGATGGCTAT ATCTTCAATG TGACCAATAA TCACAGTACA
    AAGAGCCGGT ATGTTCCCAG TGGAAAGATG ACTTCATATA CAGTCCGTGA CTTGCAGGCC
    GGCCAACGCT ACCGGATAAC AGTGACTGCA CTACGCAACA CTCCCCAGGG CCCAGTGAAC
    AGTGAGCCCA AGACAGTGCG TCCACAGACA CTGCAAAGGG AGGGACCTCA GGAGCGTCGT
    TGGACCCCCC CTAGAATTCT GCGGAACAGG CCTCCTCCAC AGCCACATCA GGAGGTGCGA
    CTGCTGACAG AAAGGGGTCC TCCTCCTGCT GATACTCCAC AGCCGCACCG ATTCACAGAG
    CTGAACGATG GGCGCCGTCG TATTTCAGCA AGGTTCAGCC ATCGTACCAA TAAAGCTATT
    AATGTTACAA AAGAGCCAGA CCCTCCAGTC AAAATTGAGA GCTCAGACGA AGCTGCTATT
    CCTGTGAGGC AGGCGCTGCA GCTACAGGAC CCTGAGCAAA GAAGCAAAGA AGGAGAACGA
    CTGGGCTGCC TGGAGACCCC GTGTCTTAAT GGAGGGACCT GTGTGAATGG AGACACTTGT
    GACTGTCCTC TGGGATTTAA AGGGCAGCAA TGCCAGTTGG CCTGCAGAAA GGAGACCTTT
    TCCTGCACAA GACTGATTTC TGAGACAACA TCACACCCAG TGTGGGAGGG AGGAATCTGC
    CACCACCTGT ACAGAAGAGT TTACAAGGTA CAGAATGATG TGTGCAGCAG GGAGGTTTGT
    GAAGGTACCA GCAGAAGCAG AGCAAGTGAG TGGACTCTTT TGGTTACAAT TACAGAAAGC
    AGAATCCCTT CCAAGCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37190
    Clone ID Related Accession (Same CDS sequence) XM_031902549.1
    Accession Version XM_031902549.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sushi, nidogen and EGF-like domain-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AACTCTTGCC ATGCTCTTCC TCTCCTTGTT TGAATGGTGG GTCCTGCGAG
    AATATTGATG CTGGGTTTCG CTGTCAGTGC GCTTTGGGAT TTACTGGAAC ACACTGTGAA
    AAAGAACCCA GCGCATGCTT TTCGTCTCCC TGTATGAATA ACGGAGTCTG CAAGGATGTG
    GGTGCTGGAT ACATCTGTGT GTGTGTTCAG GGATTCACTG GAATGTATTG TGAAACAGAG
    ATCAGGCCAT GTTCTTCAGC TCCATGTTTA AACAGAGGTG TGTGCAGAGA GAATGGCGAG
    ACTTACAGCT GTCAGTGTGC ACGCCGATTC ACCGGAAAGC ATTGCGAGAC AGAGATCAGA
    CCTTGTTTTT CTAATCCCTG TATGAACATG GGGACATGTG AGGACATGAG TGAAGGTTAT
    AGCTGTATCT GCACAGAAGG TTACACTGGA ATCCACTGTG AGACAGAGTT AAGACCCTGC
    TATTCATCCC CTTGCCTTAA TGGGGCATTG TGCAAGGACT TTGGCATTAC TTACAGCTGT
    GTGTGTGCAT GGGGGTTCAC TGGTGCACGA TGTGAGACAG AGCTGCACCC ATGCTTTTCT
    TCTCCTTGCC TGAATGGAGC AACATGCAGA GATGTCGGTT TTGCTTACAG CTGTCAGTGT
    GCACAGGGAT TCACAGGGAT GCACTGTGAG ACAGAGATCA AGCCATGTTT TTCATCCCCT
    TGTCTGAACG GAGGGACCTG TGAGGACCTG GCAATCAGCT TTGCCTGTTT ATGTGCAGAG
    AGATATACTG GCTTGCACTG CGAGACAGAG ATACAGCCTT GCTTTTCCTC TCCATGCCTG
    AATAGAGGAA TCTGTAATAA TGAAGGTGCA TTTTACAGCT GTACATGTGC AGAGGGCTTT
    ACAGGAGAAA ACTGTGAAAA AGAGGTGAGC CCTTGCCTTT CTTCTCCCTG TCTGAATGGA
    GCAACCTGCA TTGACATGGG TGCAAGTTAC AGCTGCATGT GCCCACAGGC CTTCACTGGG
    GCGCTCTGTG ACACGGAGAT ACCAAGCCCA TGTACATCAA ATCCTTGTGA GAACAAAGGT
    GTGTGCAGGG AGGCAGAAGG AGGCTACTTG TGTGACTGCC CCGCAGGTTT CAGTGGCATG
    CACTGCGAGC ACCGTGTGTC CGATGACTGC CCCTGCAGGA ATGGAGGGCG ATGTGCAGAT
    GGAAACAGCA CTTGTCACTG CCCGCCAGGA CACTTTGGGC TTCTCTGTGA ATATGAGGTC
    ACCTCGCTAC CGTGCAGCAT GGGTTCACAG TGTCCAGATG GCGGATCCTG CATGGAATAT
    GGTGGAAGTT ACTTGTGTGT GTGTCACACA GATTACATCC TTAGTAACCA CTCAATACCC
    TCACCCTGTG ACTCGGATCC CTGCCTGAAT GGTGGAACAT GTGAATCTCT AGATGATGCA
    TATACATGTG CCTGTCCCCG TGGCTTCAAT GGAAGACTTT GTGAGAAAGT AAAGCCAGCT
    CTGTGCAGCC TATCACCTTG CCGCAATGGG GGCACCTGCA AGGAGTCACG AGAAGAATAT
    TATTGCGTTT GTCCCTATCC ATTCACTGGA AAACACTGTG AGACTGGTAT GAGGGGCCCT
    TGTTCGTCAG GTCCGTGTAA TAATGGTGGT ACATGTTTCC ATTATCTTGG GAAATACAAG
    TGCGACTGCC CACCAAACTT CTCTGGGCGA CACTGTGAGA AAGCAGAATT GGACTGTGGA
    GCCCCAGAGC AGGTAAAATA TGCGAAGATG ATTGTGACAT CTACACTGCC GGGGGGTCGA
    GCAGAATATC ACTGTAATGA AGGCTATGTG CTCAGTACTC CAAACAACAC TAGTGTGTGC
    AGTGAGGAAG GGACATGGAG CTTGCCCCCA GAGTGTGAAG AAATAGACGA GTGTGCATCT
    CATCCCTGCC ATAATGGGGC ATCCTGCAAG GACCGGATTT CCCACTTTAT CTGTGAATGC
    CAGAAAGGGT ACAGTGGAAA GTATTGTGAG CAAGAAACTA ATGAGTGTCT GTCACAGCCA
    TGTAAAAATG GGGGTACCTG TCAGGATTTG CCTGGGACCT TCAAATGTAT TTGTCCTGAG
    GGTTTCACTG GATCTTATTG TGAGACAGAG GTGGATGGAT GTGACTCTTC TCCGTGCCAG
    AATGGAGGCT TGTGTGAGAA CCTGGCGGGT TCTTATCTGT GCGTGTGTCC CCGCGGCTTT
    TTTGGCTATC ACTGTCAGAC AGTGAGTGAC ATGTGCCTGT TGAACCCCTG CGGTAACCGT
    GGGGTATGTG TGTCCAGCGC AGATGGATTG ATGAGCTGCA CCTGCAGAAC GGGGTACACT
    GGAACCATGT GTGAGAAAGA GCTGCTGCCG CCATTGTCAC TGTCTCTGGG TGCGGTTGGA
    GAAAACAGTG TTTGGGTGTT CTGGACCCCC CCAGAAGATG GCACTGGGCA TGTACTGGAT
    GGGTTTGCAG TCAGTTACAC TGCCCCAGAT GGCTTATCTA GAACAGACTT TGTAGAAAGA
    GCTCACACTG AACACGAGCT GCGGGGGCTT AGTCCTGGCC GCTCTTATAA CATCACAATA
    GTAACAGTAA AGCGCAACTT GCGGCACAAT GATATCAGTC GCCCCCTCTA CCTCACAGCA
    CGAACACGGC CACCTAACGT TGACAGGCCG AGGATTAATT CTGTCATGGA CACATCTGTC
    TCAGTTTCCT GGTCACTAAG TCCTCACAGA CACACATCTA TTGCTCGTGT TCGTCTGTTT
    CTCCAGCACC CAACGGAGCC GCCCCAGCAG GCAGAGCAGG AGCCATCAGC AGGAGAATAT
    ATCTTCAGGG ATCTACGTCC TGGAGAAAAA TACAGCGTCC AAATTAGCAC CTTGAGCCGC
    TCTGACCCCA GCCACCCAGC CACAGAAAGC CTGCCCAGTG CACCTTTGAT AGCCTGGACA
    CGTCCACACC CACCTTCCAA CCTTACGGCA GCTCTTGTCA CAGCCAATTC TGTGCATCTA
    ATTTGGGAGC AACCTCCTCA TGGGTCAGTG GATGGCTATA TCTTCAATGT GACCAATAAT
    CACAGTACAA AGAGCCGGTA TGTTCCCAGT GGAAAGATGA CTTCATATAC AGTCCGTGAC
    TTGCAGGCCG GCCAACGCTA CCGGATAACA GTGACTGCAC TACGCAACAC TCCCCAGGGC
    CCAGTGAACA GTGAGCCCAA GACAGTGCGT CCACAGACAC TGCAAAGGGA GGGACCTCAG
    GAGCGTCGTT GGACCCCCCC TAGAATTCTG CGGAACAGGC CTCCTCCACA GCCACATCAG
    GAGGTGCGAC TGCTGACAGA AAGGGGTCCT CCTCCTGCTG ATACTCCACA GCCGCACCGA
    TTCACAGAGC TGAACGATGG GCGCCGTCGT ATTTCAGCAA GGTTCAGCCA TCGTACCAAT
    AAAGCTATTA ATGTTACAAA AGAGCCAGAC CCTCCAGTCA AAATTGAGAG CTCAGACGAA
    GCTGCTATTC CTGTGAGGCA GGCGCTGCAG CTACAGGACC CTGAGCAAAG AAGCAAAGAA
    GGAGAACGAC TGGGCTGCCT GGAGACCCCG TGTCTTAATG GAGGGACCTG TGTGAATGGA
    GACACTTGTG ACTGTCCTCT GGGATTTAAA GGGCAGCAAT GCCAGTTGGC CTGCAGAAAG
    GAGACCTTTT CCTGCACAAG ACTGATTTCT GAGACAACAT CACACCCAGT GTGGGAGGGA
    GGAATCTGCC ACCACCTGTA CAGAAGAGTT TACAAGGTAC AGAATGATGT GTGCAGCAGG
    GAGGTTTGTG AAGGTACCAG CAGAAGCAGA GCAAGTGAGT GGACTCTTTT GGTTACAATT
    ACAGAAAGCA GAATCCCTTC CAAGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758409.1
    CDS1295..6184
    Translation

    Target ORF information:

    RefSeq Version XM_031902549.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902549.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AACTCTTGCC ATGCTCTTCC TCTCCTTGTT TGAATGGTGG GTCCTGCGAG
    AATATTGATG CTGGGTTTCG CTGTCAGTGC GCTTTGGGAT TTACTGGAAC ACACTGTGAA
    AAAGAACCCA GCGCATGCTT TTCGTCTCCC TGTATGAATA ACGGAGTCTG CAAGGATGTG
    GGTGCTGGAT ACATCTGTGT GTGTGTTCAG GGATTCACTG GAATGTATTG TGAAACAGAG
    ATCAGGCCAT GTTCTTCAGC TCCATGTTTA AACAGAGGTG TGTGCAGAGA GAATGGCGAG
    ACTTACAGCT GTCAGTGTGC ACGCCGATTC ACCGGAAAGC ATTGCGAGAC AGAGATCAGA
    CCTTGTTTTT CTAATCCCTG TATGAACATG GGGACATGTG AGGACATGAG TGAAGGTTAT
    AGCTGTATCT GCACAGAAGG TTACACTGGA ATCCACTGTG AGACAGAGTT AAGACCCTGC
    TATTCATCCC CTTGCCTTAA TGGGGCATTG TGCAAGGACT TTGGCATTAC TTACAGCTGT
    GTGTGTGCAT GGGGGTTCAC TGGTGCACGA TGTGAGACAG AGCTGCACCC ATGCTTTTCT
    TCTCCTTGCC TGAATGGAGC AACATGCAGA GATGTCGGTT TTGCTTACAG CTGTCAGTGT
    GCACAGGGAT TCACAGGGAT GCACTGTGAG ACAGAGATCA AGCCATGTTT TTCATCCCCT
    TGTCTGAACG GAGGGACCTG TGAGGACCTG GCAATCAGCT TTGCCTGTTT ATGTGCAGAG
    AGATATACTG GCTTGCACTG CGAGACAGAG ATACAGCCTT GCTTTTCCTC TCCATGCCTG
    AATAGAGGAA TCTGTAATAA TGAAGGTGCA TTTTACAGCT GTACATGTGC AGAGGGCTTT
    ACAGGAGAAA ACTGTGAAAA AGAGGTGAGC CCTTGCCTTT CTTCTCCCTG TCTGAATGGA
    GCAACCTGCA TTGACATGGG TGCAAGTTAC AGCTGCATGT GCCCACAGGC CTTCACTGGG
    GCGCTCTGTG ACACGGAGAT ACCAAGCCCA TGTACATCAA ATCCTTGTGA GAACAAAGGT
    GTGTGCAGGG AGGCAGAAGG AGGCTACTTG TGTGACTGCC CCGCAGGTTT CAGTGGCATG
    CACTGCGAGC ACCGTGTGTC CGATGACTGC CCCTGCAGGA ATGGAGGGCG ATGTGCAGAT
    GGAAACAGCA CTTGTCACTG CCCGCCAGGA CACTTTGGGC TTCTCTGTGA ATATGAGGTC
    ACCTCGCTAC CGTGCAGCAT GGGTTCACAG TGTCCAGATG GCGGATCCTG CATGGAATAT
    GGTGGAAGTT ACTTGTGTGT GTGTCACACA GATTACATCC TTAGTAACCA CTCAATACCC
    TCACCCTGTG ACTCGGATCC CTGCCTGAAT GGTGGAACAT GTGAATCTCT AGATGATGCA
    TATACATGTG CCTGTCCCCG TGGCTTCAAT GGAAGACTTT GTGAGAAAGT AAAGCCAGCT
    CTGTGCAGCC TATCACCTTG CCGCAATGGG GGCACCTGCA AGGAGTCACG AGAAGAATAT
    TATTGCGTTT GTCCCTATCC ATTCACTGGA AAACACTGTG AGACTGGTAT GAGGGGCCCT
    TGTTCGTCAG GTCCGTGTAA TAATGGTGGT ACATGTTTCC ATTATCTTGG GAAATACAAG
    TGCGACTGCC CACCAAACTT CTCTGGGCGA CACTGTGAGA AAGCAGAATT GGACTGTGGA
    GCCCCAGAGC AGGTAAAATA TGCGAAGATG ATTGTGACAT CTACACTGCC GGGGGGTCGA
    GCAGAATATC ACTGTAATGA AGGCTATGTG CTCAGTACTC CAAACAACAC TAGTGTGTGC
    AGTGAGGAAG GGACATGGAG CTTGCCCCCA GAGTGTGAAG AAATAGACGA GTGTGCATCT
    CATCCCTGCC ATAATGGGGC ATCCTGCAAG GACCGGATTT CCCACTTTAT CTGTGAATGC
    CAGAAAGGGT ACAGTGGAAA GTATTGTGAG CAAGAAACTA ATGAGTGTCT GTCACAGCCA
    TGTAAAAATG GGGGTACCTG TCAGGATTTG CCTGGGACCT TCAAATGTAT TTGTCCTGAG
    GGTTTCACTG GATCTTATTG TGAGACAGAG GTGGATGGAT GTGACTCTTC TCCGTGCCAG
    AATGGAGGCT TGTGTGAGAA CCTGGCGGGT TCTTATCTGT GCGTGTGTCC CCGCGGCTTT
    TTTGGCTATC ACTGTCAGAC AGTGAGTGAC ATGTGCCTGT TGAACCCCTG CGGTAACCGT
    GGGGTATGTG TGTCCAGCGC AGATGGATTG ATGAGCTGCA CCTGCAGAAC GGGGTACACT
    GGAACCATGT GTGAGAAAGA GCTGCTGCCG CCATTGTCAC TGTCTCTGGG TGCGGTTGGA
    GAAAACAGTG TTTGGGTGTT CTGGACCCCC CCAGAAGATG GCACTGGGCA TGTACTGGAT
    GGGTTTGCAG TCAGTTACAC TGCCCCAGAT GGCTTATCTA GAACAGACTT TGTAGAAAGA
    GCTCACACTG AACACGAGCT GCGGGGGCTT AGTCCTGGCC GCTCTTATAA CATCACAATA
    GTAACAGTAA AGCGCAACTT GCGGCACAAT GATATCAGTC GCCCCCTCTA CCTCACAGCA
    CGAACACGGC CACCTAACGT TGACAGGCCG AGGATTAATT CTGTCATGGA CACATCTGTC
    TCAGTTTCCT GGTCACTAAG TCCTCACAGA CACACATCTA TTGCTCGTGT TCGTCTGTTT
    CTCCAGCACC CAACGGAGCC GCCCCAGCAG GCAGAGCAGG AGCCATCAGC AGGAGAATAT
    ATCTTCAGGG ATCTACGTCC TGGAGAAAAA TACAGCGTCC AAATTAGCAC CTTGAGCCGC
    TCTGACCCCA GCCACCCAGC CACAGAAAGC CTGCCCAGTG CACCTTTGAT AGCCTGGACA
    CGTCCACACC CACCTTCCAA CCTTACGGCA GCTCTTGTCA CAGCCAATTC TGTGCATCTA
    ATTTGGGAGC AACCTCCTCA TGGGTCAGTG GATGGCTATA TCTTCAATGT GACCAATAAT
    CACAGTACAA AGAGCCGGTA TGTTCCCAGT GGAAAGATGA CTTCATATAC AGTCCGTGAC
    TTGCAGGCCG GCCAACGCTA CCGGATAACA GTGACTGCAC TACGCAACAC TCCCCAGGGC
    CCAGTGAACA GTGAGCCCAA GACAGTGCGT CCACAGACAC TGCAAAGGGA GGGACCTCAG
    GAGCGTCGTT GGACCCCCCC TAGAATTCTG CGGAACAGGC CTCCTCCACA GCCACATCAG
    GAGGTGCGAC TGCTGACAGA AAGGGGTCCT CCTCCTGCTG ATACTCCACA GCCGCACCGA
    TTCACAGAGC TGAACGATGG GCGCCGTCGT ATTTCAGCAA GGTTCAGCCA TCGTACCAAT
    AAAGCTATTA ATGTTACAAA AGAGCCAGAC CCTCCAGTCA AAATTGAGAG CTCAGACGAA
    GCTGCTATTC CTGTGAGGCA GGCGCTGCAG CTACAGGACC CTGAGCAAAG AAGCAAAGAA
    GGAGAACGAC TGGGCTGCCT GGAGACCCCG TGTCTTAATG GAGGGACCTG TGTGAATGGA
    GACACTTGTG ACTGTCCTCT GGGATTTAAA GGGCAGCAAT GCCAGTTGGC CTGCAGAAAG
    GAGACCTTTT CCTGCACAAG ACTGATTTCT GAGACAACAT CACACCCAGT GTGGGAGGGA
    GGAATCTGCC ACCACCTGTA CAGAAGAGTT TACAAGGTAC AGAATGATGT GTGCAGCAGG
    GAGGTTTGTG AAGGTACCAG CAGAAGCAGA GCAAGTGAGT GGACTCTTTT GGTTACAATT
    ACAGAAAGCA GAATCCCTTC CAAGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37191
    Clone ID Related Accession (Same CDS sequence) XM_012970833.3 , XM_012970833.2
    Accession Version XM_012970833.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sushi, nidogen and EGF-like domain-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970833.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA
    GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA
    TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG
    GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAACTA ATGAGTGTCT GTCACAGCCA
    TGTAAAAATG GGGGTACCTG TCAGGATTTG CCTGGGACCT TCAAATGTAT TTGTCCTGAG
    GGTTTCACTG GATCTTATTG TGAGACAGAG GTGGATGGAT GTGACTCTTC TCCGTGCCAG
    AATGGAGGCT TGTGTGAGAA CCTGGCGGGT TCTTATCTGT GCGTGTGTCC CCGCGGCTTT
    TTTGGCTATC ACTGTCAGAC AGTGAGTGAC ATGTGCCTGT TGAACCCCTG CGGTAACCGT
    GGGGTATGTG TGTCCAGCGC AGATGGATTG ATGAGCTGCA CCTGCAGAAC GGGGTACACT
    GGAACCATGT GTGAGAAAGA GCTGCTGCCG CCATTGTCAC TGTCTCTGGG TGCGGTTGGA
    GAAAACAGTG TTTGGGTGTT CTGGACCCCC CCAGAAGATG GCACTGGGCA TGTACTGGAT
    GGGTTTGCAG TCAGTTACAC TGCCCCAGAT GGCTTATCTA GAACAGACTT TGTAGAAAGA
    GCTCACACTG AACACGAGCT GCGGGGGCTT AGTCCTGGCC GCTCTTATAA CATCACAATA
    GTAACAGTAA AGCGCAACTT GCGGCACAAT GATATCAGTC GCCCCCTCTA CCTCACAGCA
    CGAACACGGC CACCTAACGT TGACAGGCCG AGGATTAATT CTGTCATGGA CACATCTGTC
    TCAGTTTCCT GGTCACTAAG TCCTCACAGA CACACATCTA TTGCTCGTGT TCGTCTGTTT
    CTCCAGCACC CAACGGAGCC GCCCCAGCAG GCAGAGCAGG AGCCATCAGC AGGAGAATAT
    ATCTTCAGGG ATCTACGTCC TGGAGAAAAA TACAGCGTCC AAATTAGCAC CTTGAGCCGC
    TCTGACCCCA GCCACCCAGC CACAGAAAGC CTGCCCAGTG CACCTTTGAT AGCCTGGACA
    CGTCCACACC CACCTTCCAA CCTTACGGCA GCTCTTGTCA CAGCCAATTC TGTGCATCTA
    ATTTGGGAGC AACCTCCTCA TGGGTCAGTG GATGGCTATA TCTTCAATGT GACCAATAAT
    CACAGTACAA AGAGCCGGTA TGTTCCCAGT GGAAAGATGA CTTCATATAC AGTCCGTGAC
    TTGCAGGCCG GCCAACGCTA CCGGATAACA GTGACTGCAC TACGCAACAC TCCCCAGGGC
    CCAGTGAACA GTGAGCCCAA GACAGTGCGT CCACAGACAC TGCAAAGGGA GGGACCTCAG
    GAGCGTCGTT GGACCCCCCC TAGAATTCTG CGGAACAGGC CTCCTCCACA GCCACATCAG
    GAGGTGCGAC TGCTGACAGA AAGGGGTCCT CCTCCTGCTG ATACTCCACA GCCGCACCGA
    TTCACAGAGC TGAACGATGG GCGCCGTCGT ATTTCAGCAA GGTTCAGCCA TCGTACCAAT
    AAAGCTATTA ATGTTACAAA AGAGCCAGAC CCTCCAGTCA AAATTGAGAG CTCAGACGAA
    GCTGCTATTC CTGTGAGGCA GGCGCTGCAG CTACAGGACC CTGAGCAAAG AAGCAAAGAA
    GGAGAACGAC TGGGCTGCCT GGAGACCCCG TGTCTTAATG GAGGGACCTG TGTGAATGGA
    GACACTTGTG ACTGTCCTCT GGGATTTAAA GGGCAGCAAT GCCAGTTGGC CTGCAGAAAG
    GAGACCTTTT CCTGCACAAG ACTGATTTCT GAGACAACAT CACACCCAGT GTGGGAGGGA
    GGAATCTGCC ACCACCTGTA CAGAAGAGTT TACAAGGTAC AGAATGATGT GTGCAGCAGG
    GAGGTTTGTG AAGGTACCAG CAGAAGCAGA GCAAGTGAGT GGACTCTTTT GGTTACAATT
    ACAGAAAGCA GAATCCCTTC CAAGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826287.1
    CDS1299..6188
    Translation

    Target ORF information:

    RefSeq Version XM_012970833.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970833.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA
    GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA
    TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG
    GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAACTA ATGAGTGTCT GTCACAGCCA
    TGTAAAAATG GGGGTACCTG TCAGGATTTG CCTGGGACCT TCAAATGTAT TTGTCCTGAG
    GGTTTCACTG GATCTTATTG TGAGACAGAG GTGGATGGAT GTGACTCTTC TCCGTGCCAG
    AATGGAGGCT TGTGTGAGAA CCTGGCGGGT TCTTATCTGT GCGTGTGTCC CCGCGGCTTT
    TTTGGCTATC ACTGTCAGAC AGTGAGTGAC ATGTGCCTGT TGAACCCCTG CGGTAACCGT
    GGGGTATGTG TGTCCAGCGC AGATGGATTG ATGAGCTGCA CCTGCAGAAC GGGGTACACT
    GGAACCATGT GTGAGAAAGA GCTGCTGCCG CCATTGTCAC TGTCTCTGGG TGCGGTTGGA
    GAAAACAGTG TTTGGGTGTT CTGGACCCCC CCAGAAGATG GCACTGGGCA TGTACTGGAT
    GGGTTTGCAG TCAGTTACAC TGCCCCAGAT GGCTTATCTA GAACAGACTT TGTAGAAAGA
    GCTCACACTG AACACGAGCT GCGGGGGCTT AGTCCTGGCC GCTCTTATAA CATCACAATA
    GTAACAGTAA AGCGCAACTT GCGGCACAAT GATATCAGTC GCCCCCTCTA CCTCACAGCA
    CGAACACGGC CACCTAACGT TGACAGGCCG AGGATTAATT CTGTCATGGA CACATCTGTC
    TCAGTTTCCT GGTCACTAAG TCCTCACAGA CACACATCTA TTGCTCGTGT TCGTCTGTTT
    CTCCAGCACC CAACGGAGCC GCCCCAGCAG GCAGAGCAGG AGCCATCAGC AGGAGAATAT
    ATCTTCAGGG ATCTACGTCC TGGAGAAAAA TACAGCGTCC AAATTAGCAC CTTGAGCCGC
    TCTGACCCCA GCCACCCAGC CACAGAAAGC CTGCCCAGTG CACCTTTGAT AGCCTGGACA
    CGTCCACACC CACCTTCCAA CCTTACGGCA GCTCTTGTCA CAGCCAATTC TGTGCATCTA
    ATTTGGGAGC AACCTCCTCA TGGGTCAGTG GATGGCTATA TCTTCAATGT GACCAATAAT
    CACAGTACAA AGAGCCGGTA TGTTCCCAGT GGAAAGATGA CTTCATATAC AGTCCGTGAC
    TTGCAGGCCG GCCAACGCTA CCGGATAACA GTGACTGCAC TACGCAACAC TCCCCAGGGC
    CCAGTGAACA GTGAGCCCAA GACAGTGCGT CCACAGACAC TGCAAAGGGA GGGACCTCAG
    GAGCGTCGTT GGACCCCCCC TAGAATTCTG CGGAACAGGC CTCCTCCACA GCCACATCAG
    GAGGTGCGAC TGCTGACAGA AAGGGGTCCT CCTCCTGCTG ATACTCCACA GCCGCACCGA
    TTCACAGAGC TGAACGATGG GCGCCGTCGT ATTTCAGCAA GGTTCAGCCA TCGTACCAAT
    AAAGCTATTA ATGTTACAAA AGAGCCAGAC CCTCCAGTCA AAATTGAGAG CTCAGACGAA
    GCTGCTATTC CTGTGAGGCA GGCGCTGCAG CTACAGGACC CTGAGCAAAG AAGCAAAGAA
    GGAGAACGAC TGGGCTGCCT GGAGACCCCG TGTCTTAATG GAGGGACCTG TGTGAATGGA
    GACACTTGTG ACTGTCCTCT GGGATTTAAA GGGCAGCAAT GCCAGTTGGC CTGCAGAAAG
    GAGACCTTTT CCTGCACAAG ACTGATTTCT GAGACAACAT CACACCCAGT GTGGGAGGGA
    GGAATCTGCC ACCACCTGTA CAGAAGAGTT TACAAGGTAC AGAATGATGT GTGCAGCAGG
    GAGGTTTGTG AAGGTACCAG CAGAAGCAGA GCAAGTGAGT GGACTCTTTT GGTTACAATT
    ACAGAAAGCA GAATCCCTTC CAAGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa16046
    Clone ID Related Accession (Same CDS sequence) XM_002933337.5 , XM_002933337.4
    Accession Version XM_002933337.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4974bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sushi, nidogen and EGF-like domain-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002933337.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA
    GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA
    TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG
    GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC
    TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA
    GGGTACAGTG GAAAGTATTG TGAGCAAGAA ACTAATGAGT GTCTGTCACA GCCATGTAAA
    AATGGGGGTA CCTGTCAGGA TTTGCCTGGG ACCTTCAAAT GTATTTGTCC TGAGGGTTTC
    ACTGGATCTT ATTGTGAGAC AGAGGTGGAT GGATGTGACT CTTCTCCGTG CCAGAATGGA
    GGCTTGTGTG AGAACCTGGC GGGTTCTTAT CTGTGCGTGT GTCCCCGCGG CTTTTTTGGC
    TATCACTGTC AGACAGTGAG TGACATGTGC CTGTTGAACC CCTGCGGTAA CCGTGGGGTA
    TGTGTGTCCA GCGCAGATGG ATTGATGAGC TGCACCTGCA GAACGGGGTA CACTGGAACC
    ATGTGTGAGA AAGAGCTGCT GCCGCCATTG TCACTGTCTC TGGGTGCGGT TGGAGAAAAC
    AGTGTTTGGG TGTTCTGGAC CCCCCCAGAA GATGGCACTG GGCATGTACT GGATGGGTTT
    GCAGTCAGTT ACACTGCCCC AGATGGCTTA TCTAGAACAG ACTTTGTAGA AAGAGCTCAC
    ACTGAACACG AGCTGCGGGG GCTTAGTCCT GGCCGCTCTT ATAACATCAC AATAGTAACA
    GTAAAGCGCA ACTTGCGGCA CAATGATATC AGTCGCCCCC TCTACCTCAC AGCACGAACA
    CGGCCACCTA ACGTTGACAG GCCGAGGATT AATTCTGTCA TGGACACATC TGTCTCAGTT
    TCCTGGTCAC TAAGTCCTCA CAGACACACA TCTATTGCTC GTGTTCGTCT GTTTCTCCAG
    CACCCAACGG AGCCGCCCCA GCAGGCAGAG CAGGAGCCAT CAGCAGGAGA ATATATCTTC
    AGGGATCTAC GTCCTGGAGA AAAATACAGC GTCCAAATTA GCACCTTGAG CCGCTCTGAC
    CCCAGCCACC CAGCCACAGA AAGCCTGCCC AGTGCACCTT TGATAGCCTG GACACGTCCA
    CACCCACCTT CCAACCTTAC GGCAGCTCTT GTCACAGCCA ATTCTGTGCA TCTAATTTGG
    GAGCAACCTC CTCATGGGTC AGTGGATGGC TATATCTTCA ATGTGACCAA TAATCACAGT
    ACAAAGAGCC GGTATGTTCC CAGTGGAAAG ATGACTTCAT ATACAGTCCG TGACTTGCAG
    GCCGGCCAAC GCTACCGGAT AACAGTGACT GCACTACGCA ACACTCCCCA GGGCCCAGTG
    AACAGTGAGC CCAAGACAGT GCGTCCACAG ACACTGCAAA GGGAGGGACC TCAGGAGCGT
    CGTTGGACCC CCCCTAGAAT TCTGCGGAAC AGGCCTCCTC CACAGCCACA TCAGGAGGTG
    CGACTGCTGA CAGAAAGGGG TCCTCCTCCT GCTGATACTC CACAGCCGCA CCGATTCACA
    GAGCTGAACG ATGGGCGCCG TCGTATTTCA GCAAGGTTCA GCCATCGTAC CAATAAAGCT
    ATTAATGTTA CAAAAGAGCC AGACCCTCCA GTCAAAATTG AGAGCTCAGA CGAAGCTGCT
    ATTCCTGTGA GGCAGGCGCT GCAGCTACAG GACCCTGAGC AAAGAAGCAA AGAAGGAGAA
    CGACTGGGCT GCCTGGAGAC CCCGTGTCTT AATGGAGGGA CCTGTGTGAA TGGAGACACT
    TGTGACTGTC CTCTGGGATT TAAAGGGCAG CAATGCCAGT TGGCCTGCAG AAAGGAGACC
    TTTTCCTGCA CAAGACTGAT TTCTGAGACA ACATCACACC CAGTGTGGGA GGGAGGAATC
    TGCCACCACC TGTACAGAAG AGTTTACAAG GTACAGAATG ATGTGTGCAG CAGGGAGGTT
    TGTGAAGGTA CCAGCAGAAG CAGAGCAAAA AGCAGAATCC CTTCCAAGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002933383.2
    CDS3785..8758
    Translation

    Target ORF information:

    RefSeq Version XM_002933337.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002933337.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA
    GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA
    TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG
    GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC
    TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA
    GGGTACAGTG GAAAGTATTG TGAGCAAGAA ACTAATGAGT GTCTGTCACA GCCATGTAAA
    AATGGGGGTA CCTGTCAGGA TTTGCCTGGG ACCTTCAAAT GTATTTGTCC TGAGGGTTTC
    ACTGGATCTT ATTGTGAGAC AGAGGTGGAT GGATGTGACT CTTCTCCGTG CCAGAATGGA
    GGCTTGTGTG AGAACCTGGC GGGTTCTTAT CTGTGCGTGT GTCCCCGCGG CTTTTTTGGC
    TATCACTGTC AGACAGTGAG TGACATGTGC CTGTTGAACC CCTGCGGTAA CCGTGGGGTA
    TGTGTGTCCA GCGCAGATGG ATTGATGAGC TGCACCTGCA GAACGGGGTA CACTGGAACC
    ATGTGTGAGA AAGAGCTGCT GCCGCCATTG TCACTGTCTC TGGGTGCGGT TGGAGAAAAC
    AGTGTTTGGG TGTTCTGGAC CCCCCCAGAA GATGGCACTG GGCATGTACT GGATGGGTTT
    GCAGTCAGTT ACACTGCCCC AGATGGCTTA TCTAGAACAG ACTTTGTAGA AAGAGCTCAC
    ACTGAACACG AGCTGCGGGG GCTTAGTCCT GGCCGCTCTT ATAACATCAC AATAGTAACA
    GTAAAGCGCA ACTTGCGGCA CAATGATATC AGTCGCCCCC TCTACCTCAC AGCACGAACA
    CGGCCACCTA ACGTTGACAG GCCGAGGATT AATTCTGTCA TGGACACATC TGTCTCAGTT
    TCCTGGTCAC TAAGTCCTCA CAGACACACA TCTATTGCTC GTGTTCGTCT GTTTCTCCAG
    CACCCAACGG AGCCGCCCCA GCAGGCAGAG CAGGAGCCAT CAGCAGGAGA ATATATCTTC
    AGGGATCTAC GTCCTGGAGA AAAATACAGC GTCCAAATTA GCACCTTGAG CCGCTCTGAC
    CCCAGCCACC CAGCCACAGA AAGCCTGCCC AGTGCACCTT TGATAGCCTG GACACGTCCA
    CACCCACCTT CCAACCTTAC GGCAGCTCTT GTCACAGCCA ATTCTGTGCA TCTAATTTGG
    GAGCAACCTC CTCATGGGTC AGTGGATGGC TATATCTTCA ATGTGACCAA TAATCACAGT
    ACAAAGAGCC GGTATGTTCC CAGTGGAAAG ATGACTTCAT ATACAGTCCG TGACTTGCAG
    GCCGGCCAAC GCTACCGGAT AACAGTGACT GCACTACGCA ACACTCCCCA GGGCCCAGTG
    AACAGTGAGC CCAAGACAGT GCGTCCACAG ACACTGCAAA GGGAGGGACC TCAGGAGCGT
    CGTTGGACCC CCCCTAGAAT TCTGCGGAAC AGGCCTCCTC CACAGCCACA TCAGGAGGTG
    CGACTGCTGA CAGAAAGGGG TCCTCCTCCT GCTGATACTC CACAGCCGCA CCGATTCACA
    GAGCTGAACG ATGGGCGCCG TCGTATTTCA GCAAGGTTCA GCCATCGTAC CAATAAAGCT
    ATTAATGTTA CAAAAGAGCC AGACCCTCCA GTCAAAATTG AGAGCTCAGA CGAAGCTGCT
    ATTCCTGTGA GGCAGGCGCT GCAGCTACAG GACCCTGAGC AAAGAAGCAA AGAAGGAGAA
    CGACTGGGCT GCCTGGAGAC CCCGTGTCTT AATGGAGGGA CCTGTGTGAA TGGAGACACT
    TGTGACTGTC CTCTGGGATT TAAAGGGCAG CAATGCCAGT TGGCCTGCAG AAAGGAGACC
    TTTTCCTGCA CAAGACTGAT TTCTGAGACA ACATCACACC CAGTGTGGGA GGGAGGAATC
    TGCCACCACC TGTACAGAAG AGTTTACAAG GTACAGAATG ATGTGTGCAG CAGGGAGGTT
    TGTGAAGGTA CCAGCAGAAG CAGAGCAAAA AGCAGAATCC CTTCCAAGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37188
    Clone ID Related Accession (Same CDS sequence) XM_012970829.3 , XM_012970829.2
    Accession Version XM_012970829.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sushi, nidogen and EGF-like domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970829.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA
    GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA
    TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG
    GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC
    TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA
    GGGTACAGTG GAAAGTATTG TGAGCAAGAA ACTAATGAGT GTCTGTCACA GCCATGTAAA
    AATGGGGGTA CCTGTCAGGA TTTGCCTGGG ACCTTCAAAT GTATTTGTCC TGAGGGTTTC
    ACTGGATCTT ATTGTGAGAC AGAGGTGGAT GGATGTGACT CTTCTCCGTG CCAGAATGGA
    GGCTTGTGTG AGAACCTGGC GGGTTCTTAT CTGTGCGTGT GTCCCCGCGG CTTTTTTGGC
    TATCACTGTC AGACAGTGAG TGACATGTGC CTGTTGAACC CCTGCGGTAA CCGTGGGGTA
    TGTGTGTCCA GCGCAGATGG ATTGATGAGC TGCACCTGCA GAACGGGGTA CACTGGAACC
    ATGTGTGAGA AAGAGCTGCT GCCGCCATTG TCACTGTCTC TGGGTGCGGT TGGAGAAAAC
    AGTGTTTGGG TGTTCTGGAC CCCCCCAGAA GATGGCACTG GGCATGTACT GGATGGGTTT
    GCAGTCAGTT ACACTGCCCC AGATGGCTTA TCTAGAACAG ACTTTGTAGA AAGAGCTCAC
    ACTGAACACG AGCTGCGGGG GCTTAGTCCT GGCCGCTCTT ATAACATCAC AATAGTAACA
    GTAAAGCGCA ACTTGCGGCA CAATGATATC AGTCGCCCCC TCTACCTCAC AGCACGAACA
    CGGCCACCTA ACGTTGACAG GCCGAGGATT AATTCTGTCA TGGACACATC TGTCTCAGTT
    TCCTGGTCAC TAAGTCCTCA CAGACACACA TCTATTGCTC GTGTTCGTCT GTTTCTCCAG
    CACCCAACGG AGCCGCCCCA GCAGGCAGAG CAGGAGCCAT CAGCAGGAGA ATATATCTTC
    AGGGATCTAC GTCCTGGAGA AAAATACAGC GTCCAAATTA GCACCTTGAG CCGCTCTGAC
    CCCAGCCACC CAGCCACAGA AAGCCTGCCC AGTGCACCTT TGATAGCCTG GACACGTCCA
    CACCCACCTT CCAACCTTAC GGCAGCTCTT GTCACAGCCA ATTCTGTGCA TCTAATTTGG
    GAGCAACCTC CTCATGGGTC AGTGGATGGC TATATCTTCA ATGTGACCAA TAATCACAGT
    ACAAAGAGCC GGTATGTTCC CAGTGGAAAG ATGACTTCAT ATACAGTCCG TGACTTGCAG
    GCCGGCCAAC GCTACCGGAT AACAGTGACT GCACTACGCA ACACTCCCCA GGGCCCAGTG
    AACAGTGAGC CCAAGACAGT GCGTCCACAG ACACTGCAAA GGGAGGGACC TCAGGAGCGT
    CGTTGGACCC CCCCTAGAAT TCTGCGGAAC AGGCCTCCTC CACAGCCACA TCAGGAGGTG
    CGACTGCTGA CAGAAAGGGG TCCTCCTCCT GCTGATACTC CACAGCCGCA CCGATTCACA
    GAGCTGAACG ATGGGCGCCG TCGTATTTCA GCAAGGTTCA GCCATCGTAC CAATAAAGCT
    ATTAATGTTA CAAAAGAGCC AGACCCTCCA GTCAAAATTG AGAGCTCAGA CGAAGCTGCT
    ATTCCTGTGA GGCAGGCGCT GCAGCTACAG GACCCTGAGC AAAGAAGCAA AGAAGGAGAA
    CGACTGGGCT GCCTGGAGAC CCCGTGTCTT AATGGAGGGA CCTGTGTGAA TGGAGACACT
    TGTGACTGTC CTCTGGGATT TAAAGGGCAG CAATGCCAGT TGGCCTGCAG AAAGGAGACC
    TTTTCCTGCA CAAGACTGAT TTCTGAGACA ACATCACACC CAGTGTGGGA GGGAGGAATC
    TGCCACCACC TGTACAGAAG AGTTTACAAG GTACAGAATG ATGTGTGCAG CAGGGAGGTT
    TGTGAAGGTA CCAGCAGAAG CAGAGCAAGT GAGTGGACTC TTTTGGTTAC AATTACAGAA
    AGCAGAATCC CTTCCAAGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826283.1
    CDS3785..8788
    Translation

    Target ORF information:

    RefSeq Version XM_012970829.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970829.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA
    GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA
    TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG
    GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC
    TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA
    GGGTACAGTG GAAAGTATTG TGAGCAAGAA ACTAATGAGT GTCTGTCACA GCCATGTAAA
    AATGGGGGTA CCTGTCAGGA TTTGCCTGGG ACCTTCAAAT GTATTTGTCC TGAGGGTTTC
    ACTGGATCTT ATTGTGAGAC AGAGGTGGAT GGATGTGACT CTTCTCCGTG CCAGAATGGA
    GGCTTGTGTG AGAACCTGGC GGGTTCTTAT CTGTGCGTGT GTCCCCGCGG CTTTTTTGGC
    TATCACTGTC AGACAGTGAG TGACATGTGC CTGTTGAACC CCTGCGGTAA CCGTGGGGTA
    TGTGTGTCCA GCGCAGATGG ATTGATGAGC TGCACCTGCA GAACGGGGTA CACTGGAACC
    ATGTGTGAGA AAGAGCTGCT GCCGCCATTG TCACTGTCTC TGGGTGCGGT TGGAGAAAAC
    AGTGTTTGGG TGTTCTGGAC CCCCCCAGAA GATGGCACTG GGCATGTACT GGATGGGTTT
    GCAGTCAGTT ACACTGCCCC AGATGGCTTA TCTAGAACAG ACTTTGTAGA AAGAGCTCAC
    ACTGAACACG AGCTGCGGGG GCTTAGTCCT GGCCGCTCTT ATAACATCAC AATAGTAACA
    GTAAAGCGCA ACTTGCGGCA CAATGATATC AGTCGCCCCC TCTACCTCAC AGCACGAACA
    CGGCCACCTA ACGTTGACAG GCCGAGGATT AATTCTGTCA TGGACACATC TGTCTCAGTT
    TCCTGGTCAC TAAGTCCTCA CAGACACACA TCTATTGCTC GTGTTCGTCT GTTTCTCCAG
    CACCCAACGG AGCCGCCCCA GCAGGCAGAG CAGGAGCCAT CAGCAGGAGA ATATATCTTC
    AGGGATCTAC GTCCTGGAGA AAAATACAGC GTCCAAATTA GCACCTTGAG CCGCTCTGAC
    CCCAGCCACC CAGCCACAGA AAGCCTGCCC AGTGCACCTT TGATAGCCTG GACACGTCCA
    CACCCACCTT CCAACCTTAC GGCAGCTCTT GTCACAGCCA ATTCTGTGCA TCTAATTTGG
    GAGCAACCTC CTCATGGGTC AGTGGATGGC TATATCTTCA ATGTGACCAA TAATCACAGT
    ACAAAGAGCC GGTATGTTCC CAGTGGAAAG ATGACTTCAT ATACAGTCCG TGACTTGCAG
    GCCGGCCAAC GCTACCGGAT AACAGTGACT GCACTACGCA ACACTCCCCA GGGCCCAGTG
    AACAGTGAGC CCAAGACAGT GCGTCCACAG ACACTGCAAA GGGAGGGACC TCAGGAGCGT
    CGTTGGACCC CCCCTAGAAT TCTGCGGAAC AGGCCTCCTC CACAGCCACA TCAGGAGGTG
    CGACTGCTGA CAGAAAGGGG TCCTCCTCCT GCTGATACTC CACAGCCGCA CCGATTCACA
    GAGCTGAACG ATGGGCGCCG TCGTATTTCA GCAAGGTTCA GCCATCGTAC CAATAAAGCT
    ATTAATGTTA CAAAAGAGCC AGACCCTCCA GTCAAAATTG AGAGCTCAGA CGAAGCTGCT
    ATTCCTGTGA GGCAGGCGCT GCAGCTACAG GACCCTGAGC AAAGAAGCAA AGAAGGAGAA
    CGACTGGGCT GCCTGGAGAC CCCGTGTCTT AATGGAGGGA CCTGTGTGAA TGGAGACACT
    TGTGACTGTC CTCTGGGATT TAAAGGGCAG CAATGCCAGT TGGCCTGCAG AAAGGAGACC
    TTTTCCTGCA CAAGACTGAT TTCTGAGACA ACATCACACC CAGTGTGGGA GGGAGGAATC
    TGCCACCACC TGTACAGAAG AGTTTACAAG GTACAGAATG ATGTGTGCAG CAGGGAGGTT
    TGTGAAGGTA CCAGCAGAAG CAGAGCAAGT GAGTGGACTC TTTTGGTTAC AATTACAGAA
    AGCAGAATCC CTTCCAAGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37189
    Clone ID Related Accession (Same CDS sequence) XM_012970831.3 , XM_012970831.2
    Accession Version XM_012970831.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5001bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sushi, nidogen and EGF-like domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970831.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGAAT TGGACTGTGG AGCCCCAGAG
    CAGGTAAAAT ATGCGAAGAT GATTGTGACA TCTACACTGC CGGGGGGTCG AGCAGAATAT
    CACTGTAATG AAGGCTATGT GCTCAGTACT CCAAACAACA CTAGTGTGTG CAGTGAGGAA
    GGGACATGGA GCTTGCCCCC AGAGTGTGAA GAAATAGACG AGTGTGCATC TCATCCCTGC
    CATAATGGGG CATCCTGCAA GGACCGGATT TCCCACTTTA TCTGTGAATG CCAGAAAGGG
    TACAGTGGAA AGTATTGTGA GCAAGAAACT AATGAGTGTC TGTCACAGCC ATGTAAAAAT
    GGGGGTACCT GTCAGGATTT GCCTGGGACC TTCAAATGTA TTTGTCCTGA GGGTTTCACT
    GGATCTTATT GTGAGACAGA GGTGGATGGA TGTGACTCTT CTCCGTGCCA GAATGGAGGC
    TTGTGTGAGA ACCTGGCGGG TTCTTATCTG TGCGTGTGTC CCCGCGGCTT TTTTGGCTAT
    CACTGTCAGA CAGTGAGTGA CATGTGCCTG TTGAACCCCT GCGGTAACCG TGGGGTATGT
    GTGTCCAGCG CAGATGGATT GATGAGCTGC ACCTGCAGAA CGGGGTACAC TGGAACCATG
    TGTGAGAAAG AGCTGCTGCC GCCATTGTCA CTGTCTCTGG GTGCGGTTGG AGAAAACAGT
    GTTTGGGTGT TCTGGACCCC CCCAGAAGAT GGCACTGGGC ATGTACTGGA TGGGTTTGCA
    GTCAGTTACA CTGCCCCAGA TGGCTTATCT AGAACAGACT TTGTAGAAAG AGCTCACACT
    GAACACGAGC TGCGGGGGCT TAGTCCTGGC CGCTCTTATA ACATCACAAT AGTAACAGTA
    AAGCGCAACT TGCGGCACAA TGATATCAGT CGCCCCCTCT ACCTCACAGC ACGAACACGG
    CCACCTAACG TTGACAGGCC GAGGATTAAT TCTGTCATGG ACACATCTGT CTCAGTTTCC
    TGGTCACTAA GTCCTCACAG ACACACATCT ATTGCTCGTG TTCGTCTGTT TCTCCAGCAC
    CCAACGGAGC CGCCCCAGCA GGCAGAGCAG GAGCCATCAG CAGGAGAATA TATCTTCAGG
    GATCTACGTC CTGGAGAAAA ATACAGCGTC CAAATTAGCA CCTTGAGCCG CTCTGACCCC
    AGCCACCCAG CCACAGAAAG CCTGCCCAGT GCACCTTTGA TAGCCTGGAC ACGTCCACAC
    CCACCTTCCA ACCTTACGGC AGCTCTTGTC ACAGCCAATT CTGTGCATCT AATTTGGGAG
    CAACCTCCTC ATGGGTCAGT GGATGGCTAT ATCTTCAATG TGACCAATAA TCACAGTACA
    AAGAGCCGGT ATGTTCCCAG TGGAAAGATG ACTTCATATA CAGTCCGTGA CTTGCAGGCC
    GGCCAACGCT ACCGGATAAC AGTGACTGCA CTACGCAACA CTCCCCAGGG CCCAGTGAAC
    AGTGAGCCCA AGACAGTGCG TCCACAGACA CTGCAAAGGG AGGGACCTCA GGAGCGTCGT
    TGGACCCCCC CTAGAATTCT GCGGAACAGG CCTCCTCCAC AGCCACATCA GGAGGTGCGA
    CTGCTGACAG AAAGGGGTCC TCCTCCTGCT GATACTCCAC AGCCGCACCG ATTCACAGAG
    CTGAACGATG GGCGCCGTCG TATTTCAGCA AGGTTCAGCC ATCGTACCAA TAAAGCTATT
    AATGTTACAA AAGAGCCAGA CCCTCCAGTC AAAATTGAGA GCTCAGACGA AGCTGCTATT
    CCTGTGAGGC AGGCGCTGCA GCTACAGGAC CCTGAGCAAA GAAGCAAAGA AGGAGAACGA
    CTGGGCTGCC TGGAGACCCC GTGTCTTAAT GGAGGGACCT GTGTGAATGG AGACACTTGT
    GACTGTCCTC TGGGATTTAA AGGGCAGCAA TGCCAGTTGG CCTGCAGAAA GGAGACCTTT
    TCCTGCACAA GACTGATTTC TGAGACAACA TCACACCCAG TGTGGGAGGG AGGAATCTGC
    CACCACCTGT ACAGAAGAGT TTACAAGGTA CAGAATGATG TGTGCAGCAG GGAGGTTTGT
    GAAGGTACCA GCAGAAGCAG AGCAAGTGAG TGGACTCTTT TGGTTACAAT TACAGAAAGC
    AGAATCCCTT CCAAGCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826285.1
    CDS3785..8785
    Translation

    Target ORF information:

    RefSeq Version XM_012970831.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970831.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGAAT TGGACTGTGG AGCCCCAGAG
    CAGGTAAAAT ATGCGAAGAT GATTGTGACA TCTACACTGC CGGGGGGTCG AGCAGAATAT
    CACTGTAATG AAGGCTATGT GCTCAGTACT CCAAACAACA CTAGTGTGTG CAGTGAGGAA
    GGGACATGGA GCTTGCCCCC AGAGTGTGAA GAAATAGACG AGTGTGCATC TCATCCCTGC
    CATAATGGGG CATCCTGCAA GGACCGGATT TCCCACTTTA TCTGTGAATG CCAGAAAGGG
    TACAGTGGAA AGTATTGTGA GCAAGAAACT AATGAGTGTC TGTCACAGCC ATGTAAAAAT
    GGGGGTACCT GTCAGGATTT GCCTGGGACC TTCAAATGTA TTTGTCCTGA GGGTTTCACT
    GGATCTTATT GTGAGACAGA GGTGGATGGA TGTGACTCTT CTCCGTGCCA GAATGGAGGC
    TTGTGTGAGA ACCTGGCGGG TTCTTATCTG TGCGTGTGTC CCCGCGGCTT TTTTGGCTAT
    CACTGTCAGA CAGTGAGTGA CATGTGCCTG TTGAACCCCT GCGGTAACCG TGGGGTATGT
    GTGTCCAGCG CAGATGGATT GATGAGCTGC ACCTGCAGAA CGGGGTACAC TGGAACCATG
    TGTGAGAAAG AGCTGCTGCC GCCATTGTCA CTGTCTCTGG GTGCGGTTGG AGAAAACAGT
    GTTTGGGTGT TCTGGACCCC CCCAGAAGAT GGCACTGGGC ATGTACTGGA TGGGTTTGCA
    GTCAGTTACA CTGCCCCAGA TGGCTTATCT AGAACAGACT TTGTAGAAAG AGCTCACACT
    GAACACGAGC TGCGGGGGCT TAGTCCTGGC CGCTCTTATA ACATCACAAT AGTAACAGTA
    AAGCGCAACT TGCGGCACAA TGATATCAGT CGCCCCCTCT ACCTCACAGC ACGAACACGG
    CCACCTAACG TTGACAGGCC GAGGATTAAT TCTGTCATGG ACACATCTGT CTCAGTTTCC
    TGGTCACTAA GTCCTCACAG ACACACATCT ATTGCTCGTG TTCGTCTGTT TCTCCAGCAC
    CCAACGGAGC CGCCCCAGCA GGCAGAGCAG GAGCCATCAG CAGGAGAATA TATCTTCAGG
    GATCTACGTC CTGGAGAAAA ATACAGCGTC CAAATTAGCA CCTTGAGCCG CTCTGACCCC
    AGCCACCCAG CCACAGAAAG CCTGCCCAGT GCACCTTTGA TAGCCTGGAC ACGTCCACAC
    CCACCTTCCA ACCTTACGGC AGCTCTTGTC ACAGCCAATT CTGTGCATCT AATTTGGGAG
    CAACCTCCTC ATGGGTCAGT GGATGGCTAT ATCTTCAATG TGACCAATAA TCACAGTACA
    AAGAGCCGGT ATGTTCCCAG TGGAAAGATG ACTTCATATA CAGTCCGTGA CTTGCAGGCC
    GGCCAACGCT ACCGGATAAC AGTGACTGCA CTACGCAACA CTCCCCAGGG CCCAGTGAAC
    AGTGAGCCCA AGACAGTGCG TCCACAGACA CTGCAAAGGG AGGGACCTCA GGAGCGTCGT
    TGGACCCCCC CTAGAATTCT GCGGAACAGG CCTCCTCCAC AGCCACATCA GGAGGTGCGA
    CTGCTGACAG AAAGGGGTCC TCCTCCTGCT GATACTCCAC AGCCGCACCG ATTCACAGAG
    CTGAACGATG GGCGCCGTCG TATTTCAGCA AGGTTCAGCC ATCGTACCAA TAAAGCTATT
    AATGTTACAA AAGAGCCAGA CCCTCCAGTC AAAATTGAGA GCTCAGACGA AGCTGCTATT
    CCTGTGAGGC AGGCGCTGCA GCTACAGGAC CCTGAGCAAA GAAGCAAAGA AGGAGAACGA
    CTGGGCTGCC TGGAGACCCC GTGTCTTAAT GGAGGGACCT GTGTGAATGG AGACACTTGT
    GACTGTCCTC TGGGATTTAA AGGGCAGCAA TGCCAGTTGG CCTGCAGAAA GGAGACCTTT
    TCCTGCACAA GACTGATTTC TGAGACAACA TCACACCCAG TGTGGGAGGG AGGAATCTGC
    CACCACCTGT ACAGAAGAGT TTACAAGGTA CAGAATGATG TGTGCAGCAG GGAGGTTTGT
    GAAGGTACCA GCAGAAGCAG AGCAAGTGAG TGGACTCTTT TGGTTACAAT TACAGAAAGC
    AGAATCCCTT CCAAGCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37191
    Clone ID Related Accession (Same CDS sequence) XM_012970833.3 , XM_012970833.2
    Accession Version XM_012970833.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sushi, nidogen and EGF-like domain-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970833.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA
    GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA
    TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG
    GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAACTA ATGAGTGTCT GTCACAGCCA
    TGTAAAAATG GGGGTACCTG TCAGGATTTG CCTGGGACCT TCAAATGTAT TTGTCCTGAG
    GGTTTCACTG GATCTTATTG TGAGACAGAG GTGGATGGAT GTGACTCTTC TCCGTGCCAG
    AATGGAGGCT TGTGTGAGAA CCTGGCGGGT TCTTATCTGT GCGTGTGTCC CCGCGGCTTT
    TTTGGCTATC ACTGTCAGAC AGTGAGTGAC ATGTGCCTGT TGAACCCCTG CGGTAACCGT
    GGGGTATGTG TGTCCAGCGC AGATGGATTG ATGAGCTGCA CCTGCAGAAC GGGGTACACT
    GGAACCATGT GTGAGAAAGA GCTGCTGCCG CCATTGTCAC TGTCTCTGGG TGCGGTTGGA
    GAAAACAGTG TTTGGGTGTT CTGGACCCCC CCAGAAGATG GCACTGGGCA TGTACTGGAT
    GGGTTTGCAG TCAGTTACAC TGCCCCAGAT GGCTTATCTA GAACAGACTT TGTAGAAAGA
    GCTCACACTG AACACGAGCT GCGGGGGCTT AGTCCTGGCC GCTCTTATAA CATCACAATA
    GTAACAGTAA AGCGCAACTT GCGGCACAAT GATATCAGTC GCCCCCTCTA CCTCACAGCA
    CGAACACGGC CACCTAACGT TGACAGGCCG AGGATTAATT CTGTCATGGA CACATCTGTC
    TCAGTTTCCT GGTCACTAAG TCCTCACAGA CACACATCTA TTGCTCGTGT TCGTCTGTTT
    CTCCAGCACC CAACGGAGCC GCCCCAGCAG GCAGAGCAGG AGCCATCAGC AGGAGAATAT
    ATCTTCAGGG ATCTACGTCC TGGAGAAAAA TACAGCGTCC AAATTAGCAC CTTGAGCCGC
    TCTGACCCCA GCCACCCAGC CACAGAAAGC CTGCCCAGTG CACCTTTGAT AGCCTGGACA
    CGTCCACACC CACCTTCCAA CCTTACGGCA GCTCTTGTCA CAGCCAATTC TGTGCATCTA
    ATTTGGGAGC AACCTCCTCA TGGGTCAGTG GATGGCTATA TCTTCAATGT GACCAATAAT
    CACAGTACAA AGAGCCGGTA TGTTCCCAGT GGAAAGATGA CTTCATATAC AGTCCGTGAC
    TTGCAGGCCG GCCAACGCTA CCGGATAACA GTGACTGCAC TACGCAACAC TCCCCAGGGC
    CCAGTGAACA GTGAGCCCAA GACAGTGCGT CCACAGACAC TGCAAAGGGA GGGACCTCAG
    GAGCGTCGTT GGACCCCCCC TAGAATTCTG CGGAACAGGC CTCCTCCACA GCCACATCAG
    GAGGTGCGAC TGCTGACAGA AAGGGGTCCT CCTCCTGCTG ATACTCCACA GCCGCACCGA
    TTCACAGAGC TGAACGATGG GCGCCGTCGT ATTTCAGCAA GGTTCAGCCA TCGTACCAAT
    AAAGCTATTA ATGTTACAAA AGAGCCAGAC CCTCCAGTCA AAATTGAGAG CTCAGACGAA
    GCTGCTATTC CTGTGAGGCA GGCGCTGCAG CTACAGGACC CTGAGCAAAG AAGCAAAGAA
    GGAGAACGAC TGGGCTGCCT GGAGACCCCG TGTCTTAATG GAGGGACCTG TGTGAATGGA
    GACACTTGTG ACTGTCCTCT GGGATTTAAA GGGCAGCAAT GCCAGTTGGC CTGCAGAAAG
    GAGACCTTTT CCTGCACAAG ACTGATTTCT GAGACAACAT CACACCCAGT GTGGGAGGGA
    GGAATCTGCC ACCACCTGTA CAGAAGAGTT TACAAGGTAC AGAATGATGT GTGCAGCAGG
    GAGGTTTGTG AAGGTACCAG CAGAAGCAGA GCAAGTGAGT GGACTCTTTT GGTTACAATT
    ACAGAAAGCA GAATCCCTTC CAAGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826287.1
    CDS3785..8674
    Translation

    Target ORF information:

    RefSeq Version XM_012970833.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970833.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA
    GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA
    TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG
    GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAACTA ATGAGTGTCT GTCACAGCCA
    TGTAAAAATG GGGGTACCTG TCAGGATTTG CCTGGGACCT TCAAATGTAT TTGTCCTGAG
    GGTTTCACTG GATCTTATTG TGAGACAGAG GTGGATGGAT GTGACTCTTC TCCGTGCCAG
    AATGGAGGCT TGTGTGAGAA CCTGGCGGGT TCTTATCTGT GCGTGTGTCC CCGCGGCTTT
    TTTGGCTATC ACTGTCAGAC AGTGAGTGAC ATGTGCCTGT TGAACCCCTG CGGTAACCGT
    GGGGTATGTG TGTCCAGCGC AGATGGATTG ATGAGCTGCA CCTGCAGAAC GGGGTACACT
    GGAACCATGT GTGAGAAAGA GCTGCTGCCG CCATTGTCAC TGTCTCTGGG TGCGGTTGGA
    GAAAACAGTG TTTGGGTGTT CTGGACCCCC CCAGAAGATG GCACTGGGCA TGTACTGGAT
    GGGTTTGCAG TCAGTTACAC TGCCCCAGAT GGCTTATCTA GAACAGACTT TGTAGAAAGA
    GCTCACACTG AACACGAGCT GCGGGGGCTT AGTCCTGGCC GCTCTTATAA CATCACAATA
    GTAACAGTAA AGCGCAACTT GCGGCACAAT GATATCAGTC GCCCCCTCTA CCTCACAGCA
    CGAACACGGC CACCTAACGT TGACAGGCCG AGGATTAATT CTGTCATGGA CACATCTGTC
    TCAGTTTCCT GGTCACTAAG TCCTCACAGA CACACATCTA TTGCTCGTGT TCGTCTGTTT
    CTCCAGCACC CAACGGAGCC GCCCCAGCAG GCAGAGCAGG AGCCATCAGC AGGAGAATAT
    ATCTTCAGGG ATCTACGTCC TGGAGAAAAA TACAGCGTCC AAATTAGCAC CTTGAGCCGC
    TCTGACCCCA GCCACCCAGC CACAGAAAGC CTGCCCAGTG CACCTTTGAT AGCCTGGACA
    CGTCCACACC CACCTTCCAA CCTTACGGCA GCTCTTGTCA CAGCCAATTC TGTGCATCTA
    ATTTGGGAGC AACCTCCTCA TGGGTCAGTG GATGGCTATA TCTTCAATGT GACCAATAAT
    CACAGTACAA AGAGCCGGTA TGTTCCCAGT GGAAAGATGA CTTCATATAC AGTCCGTGAC
    TTGCAGGCCG GCCAACGCTA CCGGATAACA GTGACTGCAC TACGCAACAC TCCCCAGGGC
    CCAGTGAACA GTGAGCCCAA GACAGTGCGT CCACAGACAC TGCAAAGGGA GGGACCTCAG
    GAGCGTCGTT GGACCCCCCC TAGAATTCTG CGGAACAGGC CTCCTCCACA GCCACATCAG
    GAGGTGCGAC TGCTGACAGA AAGGGGTCCT CCTCCTGCTG ATACTCCACA GCCGCACCGA
    TTCACAGAGC TGAACGATGG GCGCCGTCGT ATTTCAGCAA GGTTCAGCCA TCGTACCAAT
    AAAGCTATTA ATGTTACAAA AGAGCCAGAC CCTCCAGTCA AAATTGAGAG CTCAGACGAA
    GCTGCTATTC CTGTGAGGCA GGCGCTGCAG CTACAGGACC CTGAGCAAAG AAGCAAAGAA
    GGAGAACGAC TGGGCTGCCT GGAGACCCCG TGTCTTAATG GAGGGACCTG TGTGAATGGA
    GACACTTGTG ACTGTCCTCT GGGATTTAAA GGGCAGCAAT GCCAGTTGGC CTGCAGAAAG
    GAGACCTTTT CCTGCACAAG ACTGATTTCT GAGACAACAT CACACCCAGT GTGGGAGGGA
    GGAATCTGCC ACCACCTGTA CAGAAGAGTT TACAAGGTAC AGAATGATGT GTGCAGCAGG
    GAGGTTTGTG AAGGTACCAG CAGAAGCAGA GCAAGTGAGT GGACTCTTTT GGTTACAATT
    ACAGAAAGCA GAATCCCTTC CAAGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37192
    Clone ID Related Accession (Same CDS sequence) XM_012970834.2
    Accession Version XM_012970834.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sushi, nidogen and EGF-like domain-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970834.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA
    GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA
    TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG
    GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC
    TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA
    GGGTACAGTG GAAAGTATTG TGAGCAAGAG GTGGATGGAT GTGACTCTTC TCCGTGCCAG
    AATGGAGGCT TGTGTGAGAA CCTGGCGGGT TCTTATCTGT GCGTGTGTCC CCGCGGCTTT
    TTTGGCTATC ACTGTCAGAC AGTGAGTGAC ATGTGCCTGT TGAACCCCTG CGGTAACCGT
    GGGGTATGTG TGTCCAGCGC AGATGGATTG ATGAGCTGCA CCTGCAGAAC GGGGTACACT
    GGAACCATGT GTGAGAAAGA GCTGCTGCCG CCATTGTCAC TGTCTCTGGG TGCGGTTGGA
    GAAAACAGTG TTTGGGTGTT CTGGACCCCC CCAGAAGATG GCACTGGGCA TGTACTGGAT
    GGGTTTGCAG TCAGTTACAC TGCCCCAGAT GGCTTATCTA GAACAGACTT TGTAGAAAGA
    GCTCACACTG AACACGAGCT GCGGGGGCTT AGTCCTGGCC GCTCTTATAA CATCACAATA
    GTAACAGTAA AGCGCAACTT GCGGCACAAT GATATCAGTC GCCCCCTCTA CCTCACAGCA
    CGAACACGGC CACCTAACGT TGACAGGCCG AGGATTAATT CTGTCATGGA CACATCTGTC
    TCAGTTTCCT GGTCACTAAG TCCTCACAGA CACACATCTA TTGCTCGTGT TCGTCTGTTT
    CTCCAGCACC CAACGGAGCC GCCCCAGCAG GCAGAGCAGG AGCCATCAGC AGGAGAATAT
    ATCTTCAGGG ATCTACGTCC TGGAGAAAAA TACAGCGTCC AAATTAGCAC CTTGAGCCGC
    TCTGACCCCA GCCACCCAGC CACAGAAAGC CTGCCCAGTG CACCTTTGAT AGCCTGGACA
    CGTCCACACC CACCTTCCAA CCTTACGGCA GCTCTTGTCA CAGCCAATTC TGTGCATCTA
    ATTTGGGAGC AACCTCCTCA TGGGTCAGTG GATGGCTATA TCTTCAATGT GACCAATAAT
    CACAGTACAA AGAGCCGGTA TGTTCCCAGT GGAAAGATGA CTTCATATAC AGTCCGTGAC
    TTGCAGGCCG GCCAACGCTA CCGGATAACA GTGACTGCAC TACGCAACAC TCCCCAGGGC
    CCAGTGAACA GTGAGCCCAA GACAGTGCGT CCACAGACAC TGCAAAGGGA GGGACCTCAG
    GAGCGTCGTT GGACCCCCCC TAGAATTCTG CGGAACAGGC CTCCTCCACA GCCACATCAG
    GAGGTGCGAC TGCTGACAGA AAGGGGTCCT CCTCCTGCTG ATACTCCACA GCCGCACCGA
    TTCACAGAGC TGAACGATGG GCGCCGTCGT ATTTCAGCAA GGTTCAGCCA TCGTACCAAT
    AAAGCTATTA ATGTTACAAA AGAGCCAGAC CCTCCAGTCA AAATTGAGAG CTCAGACGAA
    GCTGCTATTC CTGTGAGGCA GGCGCTGCAG CTACAGGACC CTGAGCAAAG AAGCAAAGAA
    GGAGAACGAC TGGGCTGCCT GGAGACCCCG TGTCTTAATG GAGGGACCTG TGTGAATGGA
    GACACTTGTG ACTGTCCTCT GGGATTTAAA GGGCAGCAAT GCCAGTTGGC CTGCAGAAAG
    GAGACCTTTT CCTGCACAAG ACTGATTTCT GAGACAACAT CACACCCAGT GTGGGAGGGA
    GGAATCTGCC ACCACCTGTA CAGAAGAGTT TACAAGGTAC AGAATGATGT GTGCAGCAGG
    GAGGTTTGTG AAGGTACCAG CAGAAGCAGA GCAAGTGAGT GGACTCTTTT GGTTACAATT
    ACAGAAAGCA GAATCCCTTC CAAGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826288.1
    CDS3785..8674
    Translation

    Target ORF information:

    RefSeq Version XM_012970834.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970834.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA
    GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA
    TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG
    GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC
    TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA
    GGGTACAGTG GAAAGTATTG TGAGCAAGAG GTGGATGGAT GTGACTCTTC TCCGTGCCAG
    AATGGAGGCT TGTGTGAGAA CCTGGCGGGT TCTTATCTGT GCGTGTGTCC CCGCGGCTTT
    TTTGGCTATC ACTGTCAGAC AGTGAGTGAC ATGTGCCTGT TGAACCCCTG CGGTAACCGT
    GGGGTATGTG TGTCCAGCGC AGATGGATTG ATGAGCTGCA CCTGCAGAAC GGGGTACACT
    GGAACCATGT GTGAGAAAGA GCTGCTGCCG CCATTGTCAC TGTCTCTGGG TGCGGTTGGA
    GAAAACAGTG TTTGGGTGTT CTGGACCCCC CCAGAAGATG GCACTGGGCA TGTACTGGAT
    GGGTTTGCAG TCAGTTACAC TGCCCCAGAT GGCTTATCTA GAACAGACTT TGTAGAAAGA
    GCTCACACTG AACACGAGCT GCGGGGGCTT AGTCCTGGCC GCTCTTATAA CATCACAATA
    GTAACAGTAA AGCGCAACTT GCGGCACAAT GATATCAGTC GCCCCCTCTA CCTCACAGCA
    CGAACACGGC CACCTAACGT TGACAGGCCG AGGATTAATT CTGTCATGGA CACATCTGTC
    TCAGTTTCCT GGTCACTAAG TCCTCACAGA CACACATCTA TTGCTCGTGT TCGTCTGTTT
    CTCCAGCACC CAACGGAGCC GCCCCAGCAG GCAGAGCAGG AGCCATCAGC AGGAGAATAT
    ATCTTCAGGG ATCTACGTCC TGGAGAAAAA TACAGCGTCC AAATTAGCAC CTTGAGCCGC
    TCTGACCCCA GCCACCCAGC CACAGAAAGC CTGCCCAGTG CACCTTTGAT AGCCTGGACA
    CGTCCACACC CACCTTCCAA CCTTACGGCA GCTCTTGTCA CAGCCAATTC TGTGCATCTA
    ATTTGGGAGC AACCTCCTCA TGGGTCAGTG GATGGCTATA TCTTCAATGT GACCAATAAT
    CACAGTACAA AGAGCCGGTA TGTTCCCAGT GGAAAGATGA CTTCATATAC AGTCCGTGAC
    TTGCAGGCCG GCCAACGCTA CCGGATAACA GTGACTGCAC TACGCAACAC TCCCCAGGGC
    CCAGTGAACA GTGAGCCCAA GACAGTGCGT CCACAGACAC TGCAAAGGGA GGGACCTCAG
    GAGCGTCGTT GGACCCCCCC TAGAATTCTG CGGAACAGGC CTCCTCCACA GCCACATCAG
    GAGGTGCGAC TGCTGACAGA AAGGGGTCCT CCTCCTGCTG ATACTCCACA GCCGCACCGA
    TTCACAGAGC TGAACGATGG GCGCCGTCGT ATTTCAGCAA GGTTCAGCCA TCGTACCAAT
    AAAGCTATTA ATGTTACAAA AGAGCCAGAC CCTCCAGTCA AAATTGAGAG CTCAGACGAA
    GCTGCTATTC CTGTGAGGCA GGCGCTGCAG CTACAGGACC CTGAGCAAAG AAGCAAAGAA
    GGAGAACGAC TGGGCTGCCT GGAGACCCCG TGTCTTAATG GAGGGACCTG TGTGAATGGA
    GACACTTGTG ACTGTCCTCT GGGATTTAAA GGGCAGCAAT GCCAGTTGGC CTGCAGAAAG
    GAGACCTTTT CCTGCACAAG ACTGATTTCT GAGACAACAT CACACCCAGT GTGGGAGGGA
    GGAATCTGCC ACCACCTGTA CAGAAGAGTT TACAAGGTAC AGAATGATGT GTGCAGCAGG
    GAGGTTTGTG AAGGTACCAG CAGAAGCAGA GCAAGTGAGT GGACTCTTTT GGTTACAATT
    ACAGAAAGCA GAATCCCTTC CAAGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37193
    Clone ID Related Accession (Same CDS sequence) XM_012970835.2 , XM_012970835.1
    Accession Version XM_012970835.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sushi, nidogen and EGF-like domain-containing protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGATGGA TCTGTTGCAC ATTCACTAAC ATGGGGAAAT GCTACTCCCA GCCCTGTCAG 
    AATGACGGAA CCTGTGTGGA TGAGCCTGGA AGCTTCACCT GTCTGTGTAC CTCAGATTTT
    ACAGGAACCT TATGTGAGAC AGAGGTATCG CCCTGCACAT ATATGACCTG TCATAATGGT
    GGGGAGTGCG AGGATCATAA TGGAATTGCA GTATGCGTGT GTCAGCGAGG ATACACAGGT
    GACAACTGTG ATACAGAACT CTTGCCATGC TCTTCCTCTC CTTGTTTGAA TGGTGGGTCC
    TGCGAGAATA TTGATGCTGG GTTTCGCTGT CAGTGCGCTT TGGGATTTAC TGGAACACAC
    TGTGAAAAAG AACCCAGCGC ATGCTTTTCG TCTCCCTGTA TGAATAACGG AGTCTGCAAG
    GATGTGGGTG CTGGATACAT CTGTGTGTGT GTTCAGGGAT TCACTGGAAT GTATTGTGAA
    ACAGAGATCA GGCCATGTTC TTCAGCTCCA TGTTTAAACA GAGGTGTGTG CAGAGAGAAT
    GGCGAGACTT ACAGCTGTCA GTGTGCACGC CGATTCACCG GAAAGCATTG CGAGACAGAG
    ATCAGACCTT GTTTTTCTAA TCCCTGTATG AACATGGGGA CATGTGAGGA CATGAGTGAA
    GGTTATAGCT GTATCTGCAC AGAAGGTTAC ACTGGAATCC ACTGTGAGAC AGAGTTAAGA
    CCCTGCTATT CATCCCCTTG CCTTAATGGG GCATTGTGCA AGGACTTTGG CATTACTTAC
    AGCTGTGTGT GTGCATGGGG GTTCACTGGT GCACGATGTG AGACAGAGCT GCACCCATGC
    TTTTCTTCTC CTTGCCTGAA TGGAGCAACA TGCAGAGATG TCGGTTTTGC TTACAGCTGT
    CAGTGTGCAC AGGGATTCAC AGGGATGCAC TGTGAGACAG AGATCAAGCC ATGTTTTTCA
    TCCCCTTGTC TGAACGGAGG GACCTGTGAG GACCTGGCAA TCAGCTTTGC CTGTTTATGT
    GCAGAGAGAT ATACTGGCTT GCACTGCGAG ACAGAGATAC AGCCTTGCTT TTCCTCTCCA
    TGCCTGAATA GAGGAATCTG TAATAATGAA GGTGCATTTT ACAGCTGTAC ATGTGCAGAG
    GGCTTTACAG GAGAAAACTG TGAAAAAGAG GTGAGCCCTT GCCTTTCTTC TCCCTGTCTG
    AATGGAGCAA CCTGCATTGA CATGGGTGCA AGTTACAGCT GCATGTGCCC ACAGGCCTTC
    ACTGGGGCGC TCTGTGACAC GGAGATACCA AGCCCATGTA CATCAAATCC TTGTGAGAAC
    AAAGGTGTGT GCAGGGAGGC AGAAGGAGGC TACTTGTGTG ACTGCCCCGC AGGTTTCAGT
    GGCATGCACT GCGAGCACCG TGTGTCCGAT GACTGCCCCT GCAGGAATGG AGGGCGATGT
    GCAGATGGAA ACAGCACTTG TCACTGCCCG CCAGGACACT TTGGGCTTCT CTGTGAATAT
    GAGGTCACCT CGCTACCGTG CAGCATGGGT TCACAGTGTC CAGATGGCGG ATCCTGCATG
    GAATATGGTG GAAGTTACTT GTGTGTGTGT CACACAGATT ACATCCTTAG TAACCACTCA
    ATACCCTCAC CCTGTGACTC GGATCCCTGC CTGAATGGTG GAACATGTGA ATCTCTAGAT
    GATGCATATA CATGTGCCTG TCCCCGTGGC TTCAATGGAA GACTTTGTGA GAAAGTAAAG
    CCAGCTCTGT GCAGCCTATC ACCTTGCCGC AATGGGGGCA CCTGCAAGGA GTCACGAGAA
    GAATATTATT GCGTTTGTCC CTATCCATTC ACTGGAAAAC ACTGTGAGAC TGGTATGAGG
    GGCCCTTGTT CGTCAGGTCC GTGTAATAAT GGTGGTACAT GTTTCCATTA TCTTGGGAAA
    TACAAGTGCG ACTGCCCACC AAACTTCTCT GGGCGACACT GTGAGAAAGC AGAATTGGAC
    TGTGGAGCCC CAGAGCAGGT AAAATATGCG AAGATGATTG TGACATCTAC ACTGCCGGGG
    GGTCGAGCAG AATATCACTG TAATGAAGGC TATGTGCTCA GTACTCCAAA CAACACTAGT
    GTGTGCAGTG AGGAAGGGAC ATGGAGCTTG CCCCCAGAGT GTGAAGAAAT AGACGAGTGT
    GCATCTCATC CCTGCCATAA TGGGGCATCC TGCAAGGACC GGATTTCCCA CTTTATCTGT
    GAATGCCAGA AAGGGTACAG TGGAAAGTAT TGTGAGCAAG AAACTAATGA GTGTCTGTCA
    CAGCCATGTA AAAATGGGGG TACCTGTCAG GATTTGCCTG GGACCTTCAA ATGTATTTGT
    CCTGAGGGTT TCACTGGATC TTATTGTGAG ACAGAGGTGG ATGGATGTGA CTCTTCTCCG
    TGCCAGAATG GAGGCTTGTG TGAGAACCTG GCGGGTTCTT ATCTGTGCGT GTGTCCCCGC
    GGCTTTTTTG GCTATCACTG TCAGACAGTG AGTGACATGT GCCTGTTGAA CCCCTGCGGT
    AACCGTGGGG TATGTGTGTC CAGCGCAGAT GGATTGATGA GCTGCACCTG CAGAACGGGG
    TACACTGGAA CCATGTGTGA GAAAGAGCTG CTGCCGCCAT TGTCACTGTC TCTGGGTGCG
    GTTGGAGAAA ACAGTGTTTG GGTGTTCTGG ACCCCCCCAG AAGATGGCAC TGGGCATGTA
    CTGGATGGGT TTGCAGTCAG TTACACTGCC CCAGATGGCT TATCTAGAAC AGACTTTGTA
    GAAAGAGCTC ACACTGAACA CGAGCTGCGG GGGCTTAGTC CTGGCCGCTC TTATAACATC
    ACAATAGTAA CAGTAAAGCG CAACTTGCGG CACAATGATA TCAGTCGCCC CCTCTACCTC
    ACAGCACGAA CACGGCCACC TAACGTTGAC AGGCCGAGGA TTAATTCTGT CATGGACACA
    TCTGTCTCAG TTTCCTGGTC ACTAAGTCCT CACAGACACA CATCTATTGC TCGTGTTCGT
    CTGTTTCTCC AGCACCCAAC GGAGCCGCCC CAGCAGGCAG AGCAGGAGCC ATCAGCAGGA
    GAATATATCT TCAGGGATCT ACGTCCTGGA GAAAAATACA GCGTCCAAAT TAGCACCTTG
    AGCCGCTCTG ACCCCAGCCA CCCAGCCACA GAAAGCCTGC CCAGTGCACC TTTGATAGCC
    TGGACACGTC CACACCCACC TTCCAACCTT ACGGCAGCTC TTGTCACAGC CAATTCTGTG
    CATCTAATTT GGGAGCAACC TCCTCATGGG TCAGTGGATG GCTATATCTT CAATGTGACC
    AATAATCACA GTACAAAGAG CCGGTATGTT CCCAGTGGAA AGATGACTTC ATATACAGTC
    CGTGACTTGC AGGCCGGCCA ACGCTACCGG ATAACAGTGA CTGCACTACG CAACACTCCC
    CAGGGCCCAG TGAACAGTGA GCCCAAGACA GTGCGTCCAC AGACACTGCA AAGGGAGGGA
    CCTCAGGAGC GTCGTTGGAC CCCCCCTAGA ATTCTGCGGA ACAGGCCTCC TCCACAGCCA
    CATCAGGAGG TGCGACTGCT GACAGAAAGG GGTCCTCCTC CTGCTGATAC TCCACAGCCG
    CACCGATTCA CAGAGCTGAA CGATGGGCGC CGTCGTATTT CAGCAAGGTT CAGCCATCGT
    ACCAATAAAG CTATTAATGT TACAAAAGAG CCAGACCCTC CAGTCAAAAT TGAGAGCTCA
    GACGAAGCTG CTATTCCTGT GAGGCAGGCG CTGCAGCTAC AGGACCCTGA GCAAAGAAGC
    AAAGAAGGAG AACGACTGGG CTGCCTGGAG ACCCCGTGTC TTAATGGAGG GACCTGTGTG
    AATGGAGACA CTTGTGACTG TCCTCTGGGA TTTAAAGGGC AGCAATGCCA GTTGGCCTGC
    AGAAAGGAGA CCTTTTCCTG CACAAGACTG ATTTCTGAGA CAACATCACA CCCAGTGTGG
    GAGGGAGGAA TCTGCCACCA CCTGTACAGA AGAGTTTACA AGGTACAGAA TGATGTGTGC
    AGCAGGGAGG TTTGTGAAGG TACCAGCAGA AGCAGAGCAA GTGAGTGGAC TCTTTTGGTT
    ACAATTACAG AAAGCAGAAT CCCTTCCAAG CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826289.1
    CDS48..4163
    Translation

    Target ORF information:

    RefSeq Version XM_012970835.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970835.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGATGGA TCTGTTGCAC ATTCACTAAC ATGGGGAAAT GCTACTCCCA GCCCTGTCAG 
    AATGACGGAA CCTGTGTGGA TGAGCCTGGA AGCTTCACCT GTCTGTGTAC CTCAGATTTT
    ACAGGAACCT TATGTGAGAC AGAGGTATCG CCCTGCACAT ATATGACCTG TCATAATGGT
    GGGGAGTGCG AGGATCATAA TGGAATTGCA GTATGCGTGT GTCAGCGAGG ATACACAGGT
    GACAACTGTG ATACAGAACT CTTGCCATGC TCTTCCTCTC CTTGTTTGAA TGGTGGGTCC
    TGCGAGAATA TTGATGCTGG GTTTCGCTGT CAGTGCGCTT TGGGATTTAC TGGAACACAC
    TGTGAAAAAG AACCCAGCGC ATGCTTTTCG TCTCCCTGTA TGAATAACGG AGTCTGCAAG
    GATGTGGGTG CTGGATACAT CTGTGTGTGT GTTCAGGGAT TCACTGGAAT GTATTGTGAA
    ACAGAGATCA GGCCATGTTC TTCAGCTCCA TGTTTAAACA GAGGTGTGTG CAGAGAGAAT
    GGCGAGACTT ACAGCTGTCA GTGTGCACGC CGATTCACCG GAAAGCATTG CGAGACAGAG
    ATCAGACCTT GTTTTTCTAA TCCCTGTATG AACATGGGGA CATGTGAGGA CATGAGTGAA
    GGTTATAGCT GTATCTGCAC AGAAGGTTAC ACTGGAATCC ACTGTGAGAC AGAGTTAAGA
    CCCTGCTATT CATCCCCTTG CCTTAATGGG GCATTGTGCA AGGACTTTGG CATTACTTAC
    AGCTGTGTGT GTGCATGGGG GTTCACTGGT GCACGATGTG AGACAGAGCT GCACCCATGC
    TTTTCTTCTC CTTGCCTGAA TGGAGCAACA TGCAGAGATG TCGGTTTTGC TTACAGCTGT
    CAGTGTGCAC AGGGATTCAC AGGGATGCAC TGTGAGACAG AGATCAAGCC ATGTTTTTCA
    TCCCCTTGTC TGAACGGAGG GACCTGTGAG GACCTGGCAA TCAGCTTTGC CTGTTTATGT
    GCAGAGAGAT ATACTGGCTT GCACTGCGAG ACAGAGATAC AGCCTTGCTT TTCCTCTCCA
    TGCCTGAATA GAGGAATCTG TAATAATGAA GGTGCATTTT ACAGCTGTAC ATGTGCAGAG
    GGCTTTACAG GAGAAAACTG TGAAAAAGAG GTGAGCCCTT GCCTTTCTTC TCCCTGTCTG
    AATGGAGCAA CCTGCATTGA CATGGGTGCA AGTTACAGCT GCATGTGCCC ACAGGCCTTC
    ACTGGGGCGC TCTGTGACAC GGAGATACCA AGCCCATGTA CATCAAATCC TTGTGAGAAC
    AAAGGTGTGT GCAGGGAGGC AGAAGGAGGC TACTTGTGTG ACTGCCCCGC AGGTTTCAGT
    GGCATGCACT GCGAGCACCG TGTGTCCGAT GACTGCCCCT GCAGGAATGG AGGGCGATGT
    GCAGATGGAA ACAGCACTTG TCACTGCCCG CCAGGACACT TTGGGCTTCT CTGTGAATAT
    GAGGTCACCT CGCTACCGTG CAGCATGGGT TCACAGTGTC CAGATGGCGG ATCCTGCATG
    GAATATGGTG GAAGTTACTT GTGTGTGTGT CACACAGATT ACATCCTTAG TAACCACTCA
    ATACCCTCAC CCTGTGACTC GGATCCCTGC CTGAATGGTG GAACATGTGA ATCTCTAGAT
    GATGCATATA CATGTGCCTG TCCCCGTGGC TTCAATGGAA GACTTTGTGA GAAAGTAAAG
    CCAGCTCTGT GCAGCCTATC ACCTTGCCGC AATGGGGGCA CCTGCAAGGA GTCACGAGAA
    GAATATTATT GCGTTTGTCC CTATCCATTC ACTGGAAAAC ACTGTGAGAC TGGTATGAGG
    GGCCCTTGTT CGTCAGGTCC GTGTAATAAT GGTGGTACAT GTTTCCATTA TCTTGGGAAA
    TACAAGTGCG ACTGCCCACC AAACTTCTCT GGGCGACACT GTGAGAAAGC AGAATTGGAC
    TGTGGAGCCC CAGAGCAGGT AAAATATGCG AAGATGATTG TGACATCTAC ACTGCCGGGG
    GGTCGAGCAG AATATCACTG TAATGAAGGC TATGTGCTCA GTACTCCAAA CAACACTAGT
    GTGTGCAGTG AGGAAGGGAC ATGGAGCTTG CCCCCAGAGT GTGAAGAAAT AGACGAGTGT
    GCATCTCATC CCTGCCATAA TGGGGCATCC TGCAAGGACC GGATTTCCCA CTTTATCTGT
    GAATGCCAGA AAGGGTACAG TGGAAAGTAT TGTGAGCAAG AAACTAATGA GTGTCTGTCA
    CAGCCATGTA AAAATGGGGG TACCTGTCAG GATTTGCCTG GGACCTTCAA ATGTATTTGT
    CCTGAGGGTT TCACTGGATC TTATTGTGAG ACAGAGGTGG ATGGATGTGA CTCTTCTCCG
    TGCCAGAATG GAGGCTTGTG TGAGAACCTG GCGGGTTCTT ATCTGTGCGT GTGTCCCCGC
    GGCTTTTTTG GCTATCACTG TCAGACAGTG AGTGACATGT GCCTGTTGAA CCCCTGCGGT
    AACCGTGGGG TATGTGTGTC CAGCGCAGAT GGATTGATGA GCTGCACCTG CAGAACGGGG
    TACACTGGAA CCATGTGTGA GAAAGAGCTG CTGCCGCCAT TGTCACTGTC TCTGGGTGCG
    GTTGGAGAAA ACAGTGTTTG GGTGTTCTGG ACCCCCCCAG AAGATGGCAC TGGGCATGTA
    CTGGATGGGT TTGCAGTCAG TTACACTGCC CCAGATGGCT TATCTAGAAC AGACTTTGTA
    GAAAGAGCTC ACACTGAACA CGAGCTGCGG GGGCTTAGTC CTGGCCGCTC TTATAACATC
    ACAATAGTAA CAGTAAAGCG CAACTTGCGG CACAATGATA TCAGTCGCCC CCTCTACCTC
    ACAGCACGAA CACGGCCACC TAACGTTGAC AGGCCGAGGA TTAATTCTGT CATGGACACA
    TCTGTCTCAG TTTCCTGGTC ACTAAGTCCT CACAGACACA CATCTATTGC TCGTGTTCGT
    CTGTTTCTCC AGCACCCAAC GGAGCCGCCC CAGCAGGCAG AGCAGGAGCC ATCAGCAGGA
    GAATATATCT TCAGGGATCT ACGTCCTGGA GAAAAATACA GCGTCCAAAT TAGCACCTTG
    AGCCGCTCTG ACCCCAGCCA CCCAGCCACA GAAAGCCTGC CCAGTGCACC TTTGATAGCC
    TGGACACGTC CACACCCACC TTCCAACCTT ACGGCAGCTC TTGTCACAGC CAATTCTGTG
    CATCTAATTT GGGAGCAACC TCCTCATGGG TCAGTGGATG GCTATATCTT CAATGTGACC
    AATAATCACA GTACAAAGAG CCGGTATGTT CCCAGTGGAA AGATGACTTC ATATACAGTC
    CGTGACTTGC AGGCCGGCCA ACGCTACCGG ATAACAGTGA CTGCACTACG CAACACTCCC
    CAGGGCCCAG TGAACAGTGA GCCCAAGACA GTGCGTCCAC AGACACTGCA AAGGGAGGGA
    CCTCAGGAGC GTCGTTGGAC CCCCCCTAGA ATTCTGCGGA ACAGGCCTCC TCCACAGCCA
    CATCAGGAGG TGCGACTGCT GACAGAAAGG GGTCCTCCTC CTGCTGATAC TCCACAGCCG
    CACCGATTCA CAGAGCTGAA CGATGGGCGC CGTCGTATTT CAGCAAGGTT CAGCCATCGT
    ACCAATAAAG CTATTAATGT TACAAAAGAG CCAGACCCTC CAGTCAAAAT TGAGAGCTCA
    GACGAAGCTG CTATTCCTGT GAGGCAGGCG CTGCAGCTAC AGGACCCTGA GCAAAGAAGC
    AAAGAAGGAG AACGACTGGG CTGCCTGGAG ACCCCGTGTC TTAATGGAGG GACCTGTGTG
    AATGGAGACA CTTGTGACTG TCCTCTGGGA TTTAAAGGGC AGCAATGCCA GTTGGCCTGC
    AGAAAGGAGA CCTTTTCCTG CACAAGACTG ATTTCTGAGA CAACATCACA CCCAGTGTGG
    GAGGGAGGAA TCTGCCACCA CCTGTACAGA AGAGTTTACA AGGTACAGAA TGATGTGTGC
    AGCAGGGAGG TTTGTGAAGG TACCAGCAGA AGCAGAGCAA GTGAGTGGAC TCTTTTGGTT
    ACAATTACAG AAAGCAGAAT CCCTTCCAAG CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37194
    Clone ID Related Accession (Same CDS sequence) XM_012970836.1
    Accession Version XM_012970836.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4053bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sushi, nidogen and EGF-like domain-containing protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACGGAAC CTGTGTGGAT GAGCCTGGAA GCTTCACCTG TCTGTGTACC TCAGATTTTA 
    CAGGAACCTT ATGTGAGACA GGTATCGCCC TGCACATATA TGACCTGTCA TAATGGTGGG
    GAGTGCGAGG ATCATAATGG AATTGCAGTA TGCGTGTGTC AGCGAGGATA CACAGGTGAC
    AACTGTGATA CAGAACTCTT GCCATGCTCT TCCTCTCCTT GTTTGAATGG TGGGTCCTGC
    GAGAATATTG ATGCTGGGTT TCGCTGTCAG TGCGCTTTGG GATTTACTGG AACACACTGT
    GAAAAAGAAC CCAGCGCATG CTTTTCGTCT CCCTGTATGA ATAACGGAGT CTGCAAGGAT
    GTGGGTGCTG GATACATCTG TGTGTGTGTT CAGGGATTCA CTGGAATGTA TTGTGAAACA
    GAGATCAGGC CATGTTCTTC AGCTCCATGT TTAAACAGAG GTGTGTGCAG AGAGAATGGC
    GAGACTTACA GCTGTCAGTG TGCACGCCGA TTCACCGGAA AGCATTGCGA GACAGAGATC
    AGACCTTGTT TTTCTAATCC CTGTATGAAC ATGGGGACAT GTGAGGACAT GAGTGAAGGT
    TATAGCTGTA TCTGCACAGA AGGTTACACT GGAATCCACT GTGAGACAGA GTTAAGACCC
    TGCTATTCAT CCCCTTGCCT TAATGGGGCA TTGTGCAAGG ACTTTGGCAT TACTTACAGC
    TGTGTGTGTG CATGGGGGTT CACTGGTGCA CGATGTGAGA CAGAGCTGCA CCCATGCTTT
    TCTTCTCCTT GCCTGAATGG AGCAACATGC AGAGATGTCG GTTTTGCTTA CAGCTGTCAG
    TGTGCACAGG GATTCACAGG GATGCACTGT GAGACAGAGA TCAAGCCATG TTTTTCATCC
    CCTTGTCTGA ACGGAGGGAC CTGTGAGGAC CTGGCAATCA GCTTTGCCTG TTTATGTGCA
    GAGAGATATA CTGGCTTGCA CTGCGAGACA GAGATACAGC CTTGCTTTTC CTCTCCATGC
    CTGAATAGAG GAATCTGTAA TAATGAAGGT GCATTTTACA GCTGTACATG TGCAGAGGGC
    TTTACAGGAG AAAACTGTGA AAAAGAGGTG AGCCCTTGCC TTTCTTCTCC CTGTCTGAAT
    GGAGCAACCT GCATTGACAT GGGTGCAAGT TACAGCTGCA TGTGCCCACA GGCCTTCACT
    GGGGCGCTCT GTGACACGGA GATACCAAGC CCATGTACAT CAAATCCTTG TGAGAACAAA
    GGTGTGTGCA GGGAGGCAGA AGGAGGCTAC TTGTGTGACT GCCCCGCAGG TTTCAGTGGC
    ATGCACTGCG AGCACCGTGT GTCCGATGAC TGCCCCTGCA GGAATGGAGG GCGATGTGCA
    GATGGAAACA GCACTTGTCA CTGCCCGCCA GGACACTTTG GGCTTCTCTG TGAATATGAG
    GTCACCTCGC TACCGTGCAG CATGGGTTCA CAGTGTCCAG ATGGCGGATC CTGCATGGAA
    TATGGTGGAA GTTACTTGTG TGTGTGTCAC ACAGATTACA TCCTTAGTAA CCACTCAATA
    CCCTCACCCT GTGACTCGGA TCCCTGCCTG AATGGTGGAA CATGTGAATC TCTAGATGAT
    GCATATACAT GTGCCTGTCC CCGTGGCTTC AATGGAAGAC TTTGTGAGAA AGTAAAGCCA
    GCTCTGTGCA GCCTATCACC TTGCCGCAAT GGGGGCACCT GCAAGGAGTC ACGAGAAGAA
    TATTATTGCG TTTGTCCCTA TCCATTCACT GGAAAACACT GTGAGACTGG TATGAGGGGC
    CCTTGTTCGT CAGGTCCGTG TAATAATGGT GGTACATGTT TCCATTATCT TGGGAAATAC
    AAGTGCGACT GCCCACCAAA CTTCTCTGGG CGACACTGTG AGAAAGCAGA ATTGGACTGT
    GGAGCCCCAG AGCAGGTAAA ATATGCGAAG ATGATTGTGA CATCTACACT GCCGGGGGGT
    CGAGCAGAAT ATCACTGTAA TGAAGGCTAT GTGCTCAGTA CTCCAAACAA CACTAGTGTG
    TGCAGTGAGG AAGGGACATG GAGCTTGCCC CCAGAGTGTG AAGAAATAGA CGAGTGTGCA
    TCTCATCCCT GCCATAATGG GGCATCCTGC AAGGACCGGA TTTCCCACTT TATCTGTGAA
    TGCCAGAAAG GGTACAGTGG AAAGTATTGT GAGCAAGAAA CTAATGAGTG TCTGTCACAG
    CCATGTAAAA ATGGGGGTAC CTGTCAGGAT TTGCCTGGGA CCTTCAAATG TATTTGTCCT
    GAGGGTTTCA CTGGATCTTA TTGTGAGACA GAGGTGGATG GATGTGACTC TTCTCCGTGC
    CAGAATGGAG GCTTGTGTGA GAACCTGGCG GGTTCTTATC TGTGCGTGTG TCCCCGCGGC
    TTTTTTGGCT ATCACTGTCA GACAGTGAGT GACATGTGCC TGTTGAACCC CTGCGGTAAC
    CGTGGGGTAT GTGTGTCCAG CGCAGATGGA TTGATGAGCT GCACCTGCAG AACGGGGTAC
    ACTGGAACCA TGTGTGAGAA AGAGCTGCTG CCGCCATTGT CACTGTCTCT GGGTGCGGTT
    GGAGAAAACA GTGTTTGGGT GTTCTGGACC CCCCCAGAAG ATGGCACTGG GCATGTACTG
    GATGGGTTTG CAGTCAGTTA CACTGCCCCA GATGGCTTAT CTAGAACAGA CTTTGTAGAA
    AGAGCTCACA CTGAACACGA GCTGCGGGGG CTTAGTCCTG GCCGCTCTTA TAACATCACA
    ATAGTAACAG TAAAGCGCAA CTTGCGGCAC AATGATATCA GTCGCCCCCT CTACCTCACA
    GCACGAACAC GGCCACCTAA CGTTGACAGG CCGAGGATTA ATTCTGTCAT GGACACATCT
    GTCTCAGTTT CCTGGTCACT AAGTCCTCAC AGACACACAT CTATTGCTCG TGTTCGTCTG
    TTTCTCCAGC ACCCAACGGA GCCGCCCCAG CAGGCAGAGC AGGAGCCATC AGCAGGAGAA
    TATATCTTCA GGGATCTACG TCCTGGAGAA AAATACAGCG TCCAAATTAG CACCTTGAGC
    CGCTCTGACC CCAGCCACCC AGCCACAGAA AGCCTGCCCA GTGCACCTTT GATAGCCTGG
    ACACGTCCAC ACCCACCTTC CAACCTTACG GCAGCTCTTG TCACAGCCAA TTCTGTGCAT
    CTAATTTGGG AGCAACCTCC TCATGGGTCA GTGGATGGCT ATATCTTCAA TGTGACCAAT
    AATCACAGTA CAAAGAGCCG GTATGTTCCC AGTGGAAAGA TGACTTCATA TACAGTCCGT
    GACTTGCAGG CCGGCCAACG CTACCGGATA ACAGTGACTG CACTACGCAA CACTCCCCAG
    GGCCCAGTGA ACAGTGAGCC CAAGACAGTG CGTCCACAGA CACTGCAAAG GGAGGGACCT
    CAGGAGCGTC GTTGGACCCC CCCTAGAATT CTGCGGAACA GGCCTCCTCC ACAGCCACAT
    CAGGAGGTGC GACTGCTGAC AGAAAGGGGT CCTCCTCCTG CTGATACTCC ACAGCCGCAC
    CGATTCACAG AGCTGAACGA TGGGCGCCGT CGTATTTCAG CAAGGTTCAG CCATCGTACC
    AATAAAGCTA TTAATGTTAC AAAAGAGCCA GACCCTCCAG TCAAAATTGA GAGCTCAGAC
    GAAGCTGCTA TTCCTGTGAG GCAGGCGCTG CAGCTACAGG ACCCTGAGCA AAGAAGCAAA
    GAAGGAGAAC GACTGGGCTG CCTGGAGACC CCGTGTCTTA ATGGAGGGAC CTGTGTGAAT
    GGAGACACTT GTGACTGTCC TCTGGGATTT AAAGGGCAGC AATGCCAGTT GGCCTGCAGA
    AAGGAGACCT TTTCCTGCAC AAGACTGATT TCTGAGACAA CATCACACCC AGTGTGGGAG
    GGAGGAATCT GCCACCACCT GTACAGAAGA GTTTACAAGG TACAGAATGA TGTGTGCAGC
    AGGGAGGTTT GTGAAGGTAC CAGCAGAAGC AGAGCAAGTG AGTGGACTCT TTTGGTTACA
    ATTACAGAAA GCAGAATCCC TTCCAAGCCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826290.1
    CDS124..4176
    Translation

    Target ORF information:

    RefSeq Version XM_012970836.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970836.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACGGAAC CTGTGTGGAT GAGCCTGGAA GCTTCACCTG TCTGTGTACC TCAGATTTTA 
    CAGGAACCTT ATGTGAGACA GGTATCGCCC TGCACATATA TGACCTGTCA TAATGGTGGG
    GAGTGCGAGG ATCATAATGG AATTGCAGTA TGCGTGTGTC AGCGAGGATA CACAGGTGAC
    AACTGTGATA CAGAACTCTT GCCATGCTCT TCCTCTCCTT GTTTGAATGG TGGGTCCTGC
    GAGAATATTG ATGCTGGGTT TCGCTGTCAG TGCGCTTTGG GATTTACTGG AACACACTGT
    GAAAAAGAAC CCAGCGCATG CTTTTCGTCT CCCTGTATGA ATAACGGAGT CTGCAAGGAT
    GTGGGTGCTG GATACATCTG TGTGTGTGTT CAGGGATTCA CTGGAATGTA TTGTGAAACA
    GAGATCAGGC CATGTTCTTC AGCTCCATGT TTAAACAGAG GTGTGTGCAG AGAGAATGGC
    GAGACTTACA GCTGTCAGTG TGCACGCCGA TTCACCGGAA AGCATTGCGA GACAGAGATC
    AGACCTTGTT TTTCTAATCC CTGTATGAAC ATGGGGACAT GTGAGGACAT GAGTGAAGGT
    TATAGCTGTA TCTGCACAGA AGGTTACACT GGAATCCACT GTGAGACAGA GTTAAGACCC
    TGCTATTCAT CCCCTTGCCT TAATGGGGCA TTGTGCAAGG ACTTTGGCAT TACTTACAGC
    TGTGTGTGTG CATGGGGGTT CACTGGTGCA CGATGTGAGA CAGAGCTGCA CCCATGCTTT
    TCTTCTCCTT GCCTGAATGG AGCAACATGC AGAGATGTCG GTTTTGCTTA CAGCTGTCAG
    TGTGCACAGG GATTCACAGG GATGCACTGT GAGACAGAGA TCAAGCCATG TTTTTCATCC
    CCTTGTCTGA ACGGAGGGAC CTGTGAGGAC CTGGCAATCA GCTTTGCCTG TTTATGTGCA
    GAGAGATATA CTGGCTTGCA CTGCGAGACA GAGATACAGC CTTGCTTTTC CTCTCCATGC
    CTGAATAGAG GAATCTGTAA TAATGAAGGT GCATTTTACA GCTGTACATG TGCAGAGGGC
    TTTACAGGAG AAAACTGTGA AAAAGAGGTG AGCCCTTGCC TTTCTTCTCC CTGTCTGAAT
    GGAGCAACCT GCATTGACAT GGGTGCAAGT TACAGCTGCA TGTGCCCACA GGCCTTCACT
    GGGGCGCTCT GTGACACGGA GATACCAAGC CCATGTACAT CAAATCCTTG TGAGAACAAA
    GGTGTGTGCA GGGAGGCAGA AGGAGGCTAC TTGTGTGACT GCCCCGCAGG TTTCAGTGGC
    ATGCACTGCG AGCACCGTGT GTCCGATGAC TGCCCCTGCA GGAATGGAGG GCGATGTGCA
    GATGGAAACA GCACTTGTCA CTGCCCGCCA GGACACTTTG GGCTTCTCTG TGAATATGAG
    GTCACCTCGC TACCGTGCAG CATGGGTTCA CAGTGTCCAG ATGGCGGATC CTGCATGGAA
    TATGGTGGAA GTTACTTGTG TGTGTGTCAC ACAGATTACA TCCTTAGTAA CCACTCAATA
    CCCTCACCCT GTGACTCGGA TCCCTGCCTG AATGGTGGAA CATGTGAATC TCTAGATGAT
    GCATATACAT GTGCCTGTCC CCGTGGCTTC AATGGAAGAC TTTGTGAGAA AGTAAAGCCA
    GCTCTGTGCA GCCTATCACC TTGCCGCAAT GGGGGCACCT GCAAGGAGTC ACGAGAAGAA
    TATTATTGCG TTTGTCCCTA TCCATTCACT GGAAAACACT GTGAGACTGG TATGAGGGGC
    CCTTGTTCGT CAGGTCCGTG TAATAATGGT GGTACATGTT TCCATTATCT TGGGAAATAC
    AAGTGCGACT GCCCACCAAA CTTCTCTGGG CGACACTGTG AGAAAGCAGA ATTGGACTGT
    GGAGCCCCAG AGCAGGTAAA ATATGCGAAG ATGATTGTGA CATCTACACT GCCGGGGGGT
    CGAGCAGAAT ATCACTGTAA TGAAGGCTAT GTGCTCAGTA CTCCAAACAA CACTAGTGTG
    TGCAGTGAGG AAGGGACATG GAGCTTGCCC CCAGAGTGTG AAGAAATAGA CGAGTGTGCA
    TCTCATCCCT GCCATAATGG GGCATCCTGC AAGGACCGGA TTTCCCACTT TATCTGTGAA
    TGCCAGAAAG GGTACAGTGG AAAGTATTGT GAGCAAGAAA CTAATGAGTG TCTGTCACAG
    CCATGTAAAA ATGGGGGTAC CTGTCAGGAT TTGCCTGGGA CCTTCAAATG TATTTGTCCT
    GAGGGTTTCA CTGGATCTTA TTGTGAGACA GAGGTGGATG GATGTGACTC TTCTCCGTGC
    CAGAATGGAG GCTTGTGTGA GAACCTGGCG GGTTCTTATC TGTGCGTGTG TCCCCGCGGC
    TTTTTTGGCT ATCACTGTCA GACAGTGAGT GACATGTGCC TGTTGAACCC CTGCGGTAAC
    CGTGGGGTAT GTGTGTCCAG CGCAGATGGA TTGATGAGCT GCACCTGCAG AACGGGGTAC
    ACTGGAACCA TGTGTGAGAA AGAGCTGCTG CCGCCATTGT CACTGTCTCT GGGTGCGGTT
    GGAGAAAACA GTGTTTGGGT GTTCTGGACC CCCCCAGAAG ATGGCACTGG GCATGTACTG
    GATGGGTTTG CAGTCAGTTA CACTGCCCCA GATGGCTTAT CTAGAACAGA CTTTGTAGAA
    AGAGCTCACA CTGAACACGA GCTGCGGGGG CTTAGTCCTG GCCGCTCTTA TAACATCACA
    ATAGTAACAG TAAAGCGCAA CTTGCGGCAC AATGATATCA GTCGCCCCCT CTACCTCACA
    GCACGAACAC GGCCACCTAA CGTTGACAGG CCGAGGATTA ATTCTGTCAT GGACACATCT
    GTCTCAGTTT CCTGGTCACT AAGTCCTCAC AGACACACAT CTATTGCTCG TGTTCGTCTG
    TTTCTCCAGC ACCCAACGGA GCCGCCCCAG CAGGCAGAGC AGGAGCCATC AGCAGGAGAA
    TATATCTTCA GGGATCTACG TCCTGGAGAA AAATACAGCG TCCAAATTAG CACCTTGAGC
    CGCTCTGACC CCAGCCACCC AGCCACAGAA AGCCTGCCCA GTGCACCTTT GATAGCCTGG
    ACACGTCCAC ACCCACCTTC CAACCTTACG GCAGCTCTTG TCACAGCCAA TTCTGTGCAT
    CTAATTTGGG AGCAACCTCC TCATGGGTCA GTGGATGGCT ATATCTTCAA TGTGACCAAT
    AATCACAGTA CAAAGAGCCG GTATGTTCCC AGTGGAAAGA TGACTTCATA TACAGTCCGT
    GACTTGCAGG CCGGCCAACG CTACCGGATA ACAGTGACTG CACTACGCAA CACTCCCCAG
    GGCCCAGTGA ACAGTGAGCC CAAGACAGTG CGTCCACAGA CACTGCAAAG GGAGGGACCT
    CAGGAGCGTC GTTGGACCCC CCCTAGAATT CTGCGGAACA GGCCTCCTCC ACAGCCACAT
    CAGGAGGTGC GACTGCTGAC AGAAAGGGGT CCTCCTCCTG CTGATACTCC ACAGCCGCAC
    CGATTCACAG AGCTGAACGA TGGGCGCCGT CGTATTTCAG CAAGGTTCAG CCATCGTACC
    AATAAAGCTA TTAATGTTAC AAAAGAGCCA GACCCTCCAG TCAAAATTGA GAGCTCAGAC
    GAAGCTGCTA TTCCTGTGAG GCAGGCGCTG CAGCTACAGG ACCCTGAGCA AAGAAGCAAA
    GAAGGAGAAC GACTGGGCTG CCTGGAGACC CCGTGTCTTA ATGGAGGGAC CTGTGTGAAT
    GGAGACACTT GTGACTGTCC TCTGGGATTT AAAGGGCAGC AATGCCAGTT GGCCTGCAGA
    AAGGAGACCT TTTCCTGCAC AAGACTGATT TCTGAGACAA CATCACACCC AGTGTGGGAG
    GGAGGAATCT GCCACCACCT GTACAGAAGA GTTTACAAGG TACAGAATGA TGTGTGCAGC
    AGGGAGGTTT GTGAAGGTAC CAGCAGAAGC AGAGCAAGTG AGTGGACTCT TTTGGTTACA
    ATTACAGAAA GCAGAATCCC TTCCAAGCCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa44795
    Clone ID Related Accession (Same CDS sequence) XM_018094448.1
    Accession Version XM_018094448.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4776bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sushi, nidogen and EGF-like domain-containing protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA
    GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA
    TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG
    GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAGGTGG ATGGATGTGA CTCTTCTCCG
    TGCCAGAATG GAGGCTTGTG TGAGAACCTG GCGGGTTCTT ATCTGTGCGT GTGTCCCCGC
    GGCTTTTTTG GCTATCACTG TCAGACAGTG AGTGACATGT GCCTGTTGAA CCCCTGCGGT
    AACCGTGGGG TATGTGTGTC CAGCGCAGAT GGATTGATGA GCTGCACCTG CAGAACGGGG
    TACACTGGAA CCATGTGTGA GAAAGAGCTG CTGCCGCCAT TGTCACTGTC TCTGGGTGCG
    GTTGGAGAAA ACAGTGTTTG GGTGTTCTGG ACCCCCCCAG AAGATGGCAC TGGGCATGTA
    CTGGATGGGT TTGCAGTCAG TTACACTGCC CCAGATGGCT TATCTAGAAC AGACTTTGTA
    GAAAGAGCTC ACACTGAACA CGAGCTGCGG GGGCTTAGTC CTGGCCGCTC TTATAACATC
    ACAATAGTAA CAGTAAAGCG CAACTTGCGG CACAATGATA TCAGTCGCCC CCTCTACCTC
    ACAGCACGAA CACGGCCACC TAACGTTGAC AGGCCGAGGA TTAATTCTGT CATGGACACA
    TCTGTCTCAG TTTCCTGGTC ACTAAGTCCT CACAGACACA CATCTATTGC TCGTGTTCGT
    CTGTTTCTCC AGCACCCAAC GGAGCCGCCC CAGCAGGCAG AGCAGGAGCC ATCAGCAGGA
    GAATATATCT TCAGGGATCT ACGTCCTGGA GAAAAATACA GCGTCCAAAT TAGCACCTTG
    AGCCGCTCTG ACCCCAGCCA CCCAGCCACA GAAAGCCTGC CCAGTGCACC TTTGATAGCC
    TGGACACGTC CACACCCACC TTCCAACCTT ACGGCAGCTC TTGTCACAGC CAATTCTGTG
    CATCTAATTT GGGAGCAACC TCCTCATGGG TCAGTGGATG GCTATATCTT CAATGTGACC
    AATAATCACA GTACAAAGAG CCGGTATGTT CCCAGTGGAA AGATGACTTC ATATACAGTC
    CGTGACTTGC AGGCCGGCCA ACGCTACCGG ATAACAGTGA CTGCACTACG CAACACTCCC
    CAGGGCCCAG TGAACAGTGA GCCCAAGACA GTGCGTCCAC AGACACTGCA AAGGGAGGGA
    CCTCAGGAGC GTCGTTGGAC CCCCCCTAGA ATTCTGCGGA ACAGGCCTCC TCCACAGCCA
    CATCAGGAGG TGCGACTGCT GACAGAAAGG GGTCCTCCTC CTGCTGATAC TCCACAGCCG
    CACCGATTCA CAGAGCTGAA CGATGGGCGC CGTCGTATTT CAGCAAGGTT CAGCCATCGT
    ACCAATAAAG CTATTAATGT TACAAAAGAG CCAGACCCTC CAGTCAAAAT TGAGAGCTCA
    GACGAAGCTG CTATTCCTGT GAGGCAGGCG CTGCAGCTAC AGGACCCTGA GCAAAGAAGC
    AAAGAAGGAG AACGACTGGG CTGCCTGGAG ACCCCGTGTC TTAATGGAGG GACCTGTGTG
    AATGGAGACA CTTGTGACTG TCCTCTGGGA TTTAAAGGGC AGCAATGCCA GTTGGCCTGC
    AGAAAGGAGA CCTTTTCCTG CACAAGACTG ATTTCTGAGA CAACATCACA CCCAGTGTGG
    GAGGGAGGAA TCTGCCACCA CCTGTACAGA AGAGTTTACA AGGTACAGAA TGATGTGTGC
    AGCAGGGAGG TTTGTGAAGG TACCAGCAGA AGCAGAGCAA GTGAGTGGAC TCTTTTGGTT
    ACAATTACAG AAAGCAGAAT CCCTTCCAAG CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949937.1
    CDS3785..8560
    Translation

    Target ORF information:

    RefSeq Version XM_018094448.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094448.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA
    GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA
    TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG
    GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAGGTGG ATGGATGTGA CTCTTCTCCG
    TGCCAGAATG GAGGCTTGTG TGAGAACCTG GCGGGTTCTT ATCTGTGCGT GTGTCCCCGC
    GGCTTTTTTG GCTATCACTG TCAGACAGTG AGTGACATGT GCCTGTTGAA CCCCTGCGGT
    AACCGTGGGG TATGTGTGTC CAGCGCAGAT GGATTGATGA GCTGCACCTG CAGAACGGGG
    TACACTGGAA CCATGTGTGA GAAAGAGCTG CTGCCGCCAT TGTCACTGTC TCTGGGTGCG
    GTTGGAGAAA ACAGTGTTTG GGTGTTCTGG ACCCCCCCAG AAGATGGCAC TGGGCATGTA
    CTGGATGGGT TTGCAGTCAG TTACACTGCC CCAGATGGCT TATCTAGAAC AGACTTTGTA
    GAAAGAGCTC ACACTGAACA CGAGCTGCGG GGGCTTAGTC CTGGCCGCTC TTATAACATC
    ACAATAGTAA CAGTAAAGCG CAACTTGCGG CACAATGATA TCAGTCGCCC CCTCTACCTC
    ACAGCACGAA CACGGCCACC TAACGTTGAC AGGCCGAGGA TTAATTCTGT CATGGACACA
    TCTGTCTCAG TTTCCTGGTC ACTAAGTCCT CACAGACACA CATCTATTGC TCGTGTTCGT
    CTGTTTCTCC AGCACCCAAC GGAGCCGCCC CAGCAGGCAG AGCAGGAGCC ATCAGCAGGA
    GAATATATCT TCAGGGATCT ACGTCCTGGA GAAAAATACA GCGTCCAAAT TAGCACCTTG
    AGCCGCTCTG ACCCCAGCCA CCCAGCCACA GAAAGCCTGC CCAGTGCACC TTTGATAGCC
    TGGACACGTC CACACCCACC TTCCAACCTT ACGGCAGCTC TTGTCACAGC CAATTCTGTG
    CATCTAATTT GGGAGCAACC TCCTCATGGG TCAGTGGATG GCTATATCTT CAATGTGACC
    AATAATCACA GTACAAAGAG CCGGTATGTT CCCAGTGGAA AGATGACTTC ATATACAGTC
    CGTGACTTGC AGGCCGGCCA ACGCTACCGG ATAACAGTGA CTGCACTACG CAACACTCCC
    CAGGGCCCAG TGAACAGTGA GCCCAAGACA GTGCGTCCAC AGACACTGCA AAGGGAGGGA
    CCTCAGGAGC GTCGTTGGAC CCCCCCTAGA ATTCTGCGGA ACAGGCCTCC TCCACAGCCA
    CATCAGGAGG TGCGACTGCT GACAGAAAGG GGTCCTCCTC CTGCTGATAC TCCACAGCCG
    CACCGATTCA CAGAGCTGAA CGATGGGCGC CGTCGTATTT CAGCAAGGTT CAGCCATCGT
    ACCAATAAAG CTATTAATGT TACAAAAGAG CCAGACCCTC CAGTCAAAAT TGAGAGCTCA
    GACGAAGCTG CTATTCCTGT GAGGCAGGCG CTGCAGCTAC AGGACCCTGA GCAAAGAAGC
    AAAGAAGGAG AACGACTGGG CTGCCTGGAG ACCCCGTGTC TTAATGGAGG GACCTGTGTG
    AATGGAGACA CTTGTGACTG TCCTCTGGGA TTTAAAGGGC AGCAATGCCA GTTGGCCTGC
    AGAAAGGAGA CCTTTTCCTG CACAAGACTG ATTTCTGAGA CAACATCACA CCCAGTGTGG
    GAGGGAGGAA TCTGCCACCA CCTGTACAGA AGAGTTTACA AGGTACAGAA TGATGTGTGC
    AGCAGGGAGG TTTGTGAAGG TACCAGCAGA AGCAGAGCAA GTGAGTGGAC TCTTTTGGTT
    ACAATTACAG AAAGCAGAAT CCCTTCCAAG CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa44796
    Clone ID Related Accession (Same CDS sequence) XM_018094449.1
    Accession Version XM_018094449.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sushi, nidogen and EGF-like domain-containing protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA
    GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA
    TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG
    GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC
    TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA
    GGGTACAGTG GAAAGTATTG TGAGCAAGAA ACTAATGAGT GTCTGTCACA GCCATGTAAA
    AATGGGGGTA CCTGTCAGGA TTTGCCTGGG ACCTTCAAAT GTATTTGTCC TGAGGGTTTC
    ACTGGATCTT ATTGTGAGAC AGAGGTGGAT GGATGTGACT CTTCTCCGTG CCAGAATGGA
    GGCTTGTGTG AGAACCTGGC GGGTTCTTAT CTGTGCGTGT GTCCCCGCGG CTTTTTTGGC
    TATCACTGTC AGACAGTGAG TGACATGTGC CTGTTGAACC CCTGCGGTAA CCGTGGGGTA
    TGTGTGTCCA GCGCAGATGG ATTGATGAGC TGCACCTGCA GAACGGGGTA CACTGGAACC
    ATGTGTGAGA AAGAGCTGCT GCCGCCATTG TCACTGTCTC TGGGTGCGGT TGGAGAAAAC
    AGTGTTTGGG TGTTCTGGAC CCCCCCAGAA GATGGCACTG GGCATGTACT GGATGGGTTT
    GCAGTCAGTT ACACTGCCCC AGATGGCTTA TCTAGAACAG ACTTTGTAGA AAGAGCTCAC
    ACTGAACACG AGCTGCGGGG GCTTAGTCCT GGCCGCTCTT ATAACATCAC AATAGTAACA
    GTAAAGCGCA ACTTGCGGCA CAATGATATC AGTCGCCCCC TCTACCTCAC AGCACGAACA
    CGGCCACCTA ACGTTGACAG GCCGAGGATT AATTCTGTCA TGGACACATC TGTCTCAGTT
    TCCTGGTCAC TAAGTCCTCA CAGACACACA TCTATTGCTC GTGTTCGTCT GTTTCTCCAG
    CACCCAACGG AGCCGCCCCA GCAGGCAGAG CAGGAGCCAT CAGCAGGAGA ATATATCTTC
    AGGGATCTAC GTCCTGGAGA AAAATACAGC GTCCAAATTA GCACCTTGAG CCGCTCTGAC
    CCCAGCCACC CAGCCACAGA AAGCCTGCCC AGTGCACCTT TGATAGCCTG GACACGTCCA
    CACCCACCTT CCAACCTTAC GGCAGCTCTT GTCACAGCCA ATTCTGTGCA TCTAATTTGG
    GAGCAACCTC CTCATGGGTC AGTGGATGGC TATATCTTCA ATGTGACCAA TAATCACAGT
    ACAAAGAGCC GGTATGTTCC CAGTGGAAAG ATGACTTCAT ATACAGTCCG TGACTTGCAG
    GCCGGCCAAC GCTACCGGAT AACAGTGACT GCACTACGCA ACACTCCCCA GGGCCCAGTG
    AACAGTGAGC CCAAGACAGT GCGTCCACAG ACACGTAATG CAAAGGGAGG GACCTCAGGA
    GCGTCGTTGG ACCCCCCCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949938.1
    CDS3785..8185
    Translation

    Target ORF information:

    RefSeq Version XM_018094449.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sushi, nidogen and EGF-like domains 1 (sned1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094449.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAGGTTGC CTTCTCTTTT CTGGATTTTC ATCTCTATGG TGTGGCGTGC ACAAGGCATG 
    GTGCCTCTGG AAGATTTTTA CCCCTTTGGT ACAAAAGAGG GGGACACTGT GACCCGCAGG
    CAAGACGATG GGGGATCTGG GCTACTGCCA ATTTCCAAAG CCTTCACGTT TTTCGGTGAC
    AAGCACAGTG GTCTCTATGT GAATAACAAT GGGGTGGTGT CCTTCCTCCG GGAGGTGTCC
    CAGTTTACTC CTGTGGCATT TCCTATCTCT GGTGACCGCC GTGTGGTGGC TGCATTTTGG
    GCAGATGTGG ATAACCGTAG GGGTGGCAAT GTGTTCTACC GCGAGACAGA GGATCTTGTG
    ATTGTGGATC GTGCCACTAG GGATGTACGC ACATACTTCC CAGAACTGCC AGACTTTCAC
    TCTACTTGGG TCTTCATTGC CACTTGGCAC CGTGTCACTT TCTATGGTGG TGATTCTACT
    TCACCTGTTA ACACCTTTCA GATTGTGCTG ATCACAGATG GCCTTCGCTC CTTCACCATT
    TTTAATTATG AAACAATTGA ATGGACAACA GGCCGGCATG CCAGCAGTGG TGGAGACAGT
    TCTGGTAGAG GGGGCATCGC TGCACAGGCA GGCTTTAATG CAGGGGATGG CTGGAGGTAC
    TTTAATATCC CTGGCTCTCG CACTGATGAC ATTGCAAGAA TTGCAAACAC GACCAATGTA
    GGGGTTCCTG GACGCTGGGT GTTCCGTATA GACAACAACC ATGTGCAGGT TGGGGAATGC
    AGGAATGCAA CTTCTGTGTG CTGGACTCTA CGTCCCTGCC AGAATGGGGG TCGCTGCATC
    GATGACTGTG TGACTGGGAA TCCCTCATAC ACTTGTTCTT GCCTTAGCGG CTTCACTGGA
    AAGCACTGCC ACATTGACAT GGGGAAATGC TACTCCCAGC CCTGTCAGAA TGACGGAACC
    TGTGTGGATG AGCCTGGAAG CTTCACCTGT CTGTGTACCT CAGATTTTAC AGGAACCTTA
    TGTGAGACAG AGGTATCGCC CTGCACATAT ATGACCTGTC ATAATGGTGG GGAGTGCGAG
    GATCATAATG GAATTGCAGT ATGCGTGTGT CAGCGAGGAT ACACAGGTGA CAACTGTGAT
    ACAGAACTCT TGCCATGCTC TTCCTCTCCT TGTTTGAATG GTGGGTCCTG CGAGAATATT
    GATGCTGGGT TTCGCTGTCA GTGCGCTTTG GGATTTACTG GAACACACTG TGAAAAAGAA
    CCCAGCGCAT GCTTTTCGTC TCCCTGTATG AATAACGGAG TCTGCAAGGA TGTGGGTGCT
    GGATACATCT GTGTGTGTGT TCAGGGATTC ACTGGAATGT ATTGTGAAAC AGAGATCAGG
    CCATGTTCTT CAGCTCCATG TTTAAACAGA GGTGTGTGCA GAGAGAATGG CGAGACTTAC
    AGCTGTCAGT GTGCACGCCG ATTCACCGGA AAGCATTGCG AGACAGAGAT CAGACCTTGT
    TTTTCTAATC CCTGTATGAA CATGGGGACA TGTGAGGACA TGAGTGAAGG TTATAGCTGT
    ATCTGCACAG AAGGTTACAC TGGAATCCAC TGTGAGACAG AGTTAAGACC CTGCTATTCA
    TCCCCTTGCC TTAATGGGGC ATTGTGCAAG GACTTTGGCA TTACTTACAG CTGTGTGTGT
    GCATGGGGGT TCACTGGTGC ACGATGTGAG ACAGAGCTGC ACCCATGCTT TTCTTCTCCT
    TGCCTGAATG GAGCAACATG CAGAGATGTC GGTTTTGCTT ACAGCTGTCA GTGTGCACAG
    GGATTCACAG GGATGCACTG TGAGACAGAG ATCAAGCCAT GTTTTTCATC CCCTTGTCTG
    AACGGAGGGA CCTGTGAGGA CCTGGCAATC AGCTTTGCCT GTTTATGTGC AGAGAGATAT
    ACTGGCTTGC ACTGCGAGAC AGAGATACAG CCTTGCTTTT CCTCTCCATG CCTGAATAGA
    GGAATCTGTA ATAATGAAGG TGCATTTTAC AGCTGTACAT GTGCAGAGGG CTTTACAGGA
    GAAAACTGTG AAAAAGAGGT GAGCCCTTGC CTTTCTTCTC CCTGTCTGAA TGGAGCAACC
    TGCATTGACA TGGGTGCAAG TTACAGCTGC ATGTGCCCAC AGGCCTTCAC TGGGGCGCTC
    TGTGACACGG AGATACCAAG CCCATGTACA TCAAATCCTT GTGAGAACAA AGGTGTGTGC
    AGGGAGGCAG AAGGAGGCTA CTTGTGTGAC TGCCCCGCAG GTTTCAGTGG CATGCACTGC
    GAGCACCGTG TGTCCGATGA CTGCCCCTGC AGGAATGGAG GGCGATGTGC AGATGGAAAC
    AGCACTTGTC ACTGCCCGCC AGGACACTTT GGGCTTCTCT GTGAATATGA GGTCACCTCG
    CTACCGTGCA GCATGGGTTC ACAGTGTCCA GATGGCGGAT CCTGCATGGA ATATGGTGGA
    AGTTACTTGT GTGTGTGTCA CACAGATTAC ATCCTTAGTA ACCACTCAAT ACCCTCACCC
    TGTGACTCGG ATCCCTGCCT GAATGGTGGA ACATGTGAAT CTCTAGATGA TGCATATACA
    TGTGCCTGTC CCCGTGGCTT CAATGGAAGA CTTTGTGAGA AAGTAAAGCC AGCTCTGTGC
    AGCCTATCAC CTTGCCGCAA TGGGGGCACC TGCAAGGAGT CACGAGAAGA ATATTATTGC
    GTTTGTCCCT ATCCATTCAC TGGAAAACAC TGTGAGACTG GTATGAGGGG CCCTTGTTCG
    TCAGGTCCGT GTAATAATGG TGGTACATGT TTCCATTATC TTGGGAAATA CAAGTGCGAC
    TGCCCACCAA ACTTCTCTGG GCGACACTGT GAGAAAGCAG AATTGGACTG TGGAGCCCCA
    GAGCAGGTAA AATATGCGAA GATGATTGTG ACATCTACAC TGCCGGGGGG TCGAGCAGAA
    TATCACTGTA ATGAAGGCTA TGTGCTCAGT ACTCCAAACA ACACTAGTGT GTGCAGTGAG
    GAAGGGACAT GGAGCTTGCC CCCAGAGTGT GAAGAAATAG ACGAGTGTGC ATCTCATCCC
    TGCCATAATG GGGCATCCTG CAAGGACCGG ATTTCCCACT TTATCTGTGA ATGCCAGAAA
    GGGTACAGTG GAAAGTATTG TGAGCAAGAA ACTAATGAGT GTCTGTCACA GCCATGTAAA
    AATGGGGGTA CCTGTCAGGA TTTGCCTGGG ACCTTCAAAT GTATTTGTCC TGAGGGTTTC
    ACTGGATCTT ATTGTGAGAC AGAGGTGGAT GGATGTGACT CTTCTCCGTG CCAGAATGGA
    GGCTTGTGTG AGAACCTGGC GGGTTCTTAT CTGTGCGTGT GTCCCCGCGG CTTTTTTGGC
    TATCACTGTC AGACAGTGAG TGACATGTGC CTGTTGAACC CCTGCGGTAA CCGTGGGGTA
    TGTGTGTCCA GCGCAGATGG ATTGATGAGC TGCACCTGCA GAACGGGGTA CACTGGAACC
    ATGTGTGAGA AAGAGCTGCT GCCGCCATTG TCACTGTCTC TGGGTGCGGT TGGAGAAAAC
    AGTGTTTGGG TGTTCTGGAC CCCCCCAGAA GATGGCACTG GGCATGTACT GGATGGGTTT
    GCAGTCAGTT ACACTGCCCC AGATGGCTTA TCTAGAACAG ACTTTGTAGA AAGAGCTCAC
    ACTGAACACG AGCTGCGGGG GCTTAGTCCT GGCCGCTCTT ATAACATCAC AATAGTAACA
    GTAAAGCGCA ACTTGCGGCA CAATGATATC AGTCGCCCCC TCTACCTCAC AGCACGAACA
    CGGCCACCTA ACGTTGACAG GCCGAGGATT AATTCTGTCA TGGACACATC TGTCTCAGTT
    TCCTGGTCAC TAAGTCCTCA CAGACACACA TCTATTGCTC GTGTTCGTCT GTTTCTCCAG
    CACCCAACGG AGCCGCCCCA GCAGGCAGAG CAGGAGCCAT CAGCAGGAGA ATATATCTTC
    AGGGATCTAC GTCCTGGAGA AAAATACAGC GTCCAAATTA GCACCTTGAG CCGCTCTGAC
    CCCAGCCACC CAGCCACAGA AAGCCTGCCC AGTGCACCTT TGATAGCCTG GACACGTCCA
    CACCCACCTT CCAACCTTAC GGCAGCTCTT GTCACAGCCA ATTCTGTGCA TCTAATTTGG
    GAGCAACCTC CTCATGGGTC AGTGGATGGC TATATCTTCA ATGTGACCAA TAATCACAGT
    ACAAAGAGCC GGTATGTTCC CAGTGGAAAG ATGACTTCAT ATACAGTCCG TGACTTGCAG
    GCCGGCCAAC GCTACCGGAT AACAGTGACT GCACTACGCA ACACTCCCCA GGGCCCAGTG
    AACAGTGAGC CCAAGACAGT GCGTCCACAG ACACGTAATG CAAAGGGAGG GACCTCAGGA
    GCGTCGTTGG ACCCCCCCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.