tnrc6b gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012961478.2	XP_012816932.1	trinucleotide repeat-containing gene 6B protein isoform X3
XM_031901080.1	XP_031756940.1	trinucleotide repeat-containing gene 6B protein isoform X1
XM_031901082.1	XP_031756942.1	trinucleotide repeat-containing gene 6B protein isoform X3
XM_012961481.3	XP_012816935.2	trinucleotide repeat-containing gene 6B protein isoform X6
XM_018093653.1	XP_017949142.1	trinucleotide repeat-containing gene 6B protein isoform X1
XM_012961481.2	XP_012816935.1	trinucleotide repeat-containing gene 6B protein isoform X4
XM_012961477.3	XP_012816931.2	trinucleotide repeat-containing gene 6B protein isoform X4
XM_002934209.5	XP_002934255.3	trinucleotide repeat-containing gene 6B protein isoform X4
XM_018093655.1	XP_017949144.1	trinucleotide repeat-containing gene 6B protein isoform X5
XM_012961478.3	XP_012816932.2	trinucleotide repeat-containing gene 6B protein isoform X4
XM_018093654.1	XP_017949143.1	trinucleotide repeat-containing gene 6B protein isoform X2
XM_012961477.2	XP_012816931.1	trinucleotide repeat-containing gene 6B protein isoform X3
XM_002934209.4	XP_002934255.2	trinucleotide repeat-containing gene 6B protein isoform X3
XM_018093656.1	XP_017949145.1	trinucleotide repeat-containing gene 6B protein isoform X6
XM_031901083.1	XP_031756943.1	trinucleotide repeat-containing gene 6B protein isoform X5
XM_031901081.1	XP_031756941.1	trinucleotide repeat-containing gene 6B protein isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa57432	XM_031901080.1 Latest version!	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57433	XM_031901081.1 Latest version!	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57434	XM_031901082.1 Latest version!	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57435	XM_012961477.3 Latest version!	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57435	XM_012961478.3 Latest version!	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57435	XM_002934209.5 Latest version!	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57436	XM_031901083.1 Latest version!	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57437	XM_012961481.3 Latest version!	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa09307	XM_012961478.2 Latest version!	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OXa09307	XM_002934209.4 Latest version!	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OXa09307	XM_012961477.2 Latest version!	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OXa37107	XM_012961481.2 Latest version!	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OXa44221	XM_018093653.1 Latest version!	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OXa44222	XM_018093654.1 Latest version!	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OXa44223	XM_018093655.1 Latest version!	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OXa44224	XM_018093656.1 Latest version!	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

tnrc6b ( XM_031901080.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756940.1 Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa57432
Clone ID Related Accession (Same CDS sequence)	XM_031901080.1
Accession Version	XM_031901080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trinucleotide repeat-containing gene 6B protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAATG GCTGCCAGTC TCTCGTTTAT GCACATTTCC CTTTAAAGAG GAGAAAACAG 
AAATATATAA ACAAGTGTGT TTGGAATTTA TTATTGTGTA TTGCATTTTT GCCTATTTTT 
ATTTTGTGTA CTTGCGTTCA TTTTTTTTGT ACTGGATTTA CCTTGTATGC CTCAAACCGC 
TGGATTATAG CACAGTTGCA CTTTATAAGA GAGAAGGAGC AAGAAAGGGA AGAACAGTTA 
ATGGAAGACA AGAAAAGGAA GAAAGAGGAT AAAAAGAAGA AAGAAGCTGC TCAGAAGGTC 
ACAGAACAAA AAACCAAAGT GCCCGAAGTG ACGAAACCAA GTTCAAGCCA AGCGACGGCC 
GCCAGCCTAC CTGGCAGCTC CCCCTCGCCA CCAGTCAATG GTGGCAACAA TGCCAAAAGG 
GTGGCAGTGC CGAACGGACA ACCGCCCAGC ACCGCCCGCT ATATGCCTCG GGAGGTGCCG 
CCGCGATTCC GCTGCCAGCA GGACCACAAA GTGTTACTGA AGCGTGGACA GCCTCCTCCA 
GCGTCCTGTA TGCTGCCGGG GGGAGGAGCA GGGACTCCCC CCTCAACCAC TGCTCCAGGG 
GCCGGCCCAG GCAACGCACA GCCAGGGGCG GGGACACTGC TGCCAGGGGA AAGCGGGGCT 
TCAACAGACT CATCATCGGG GGCAGCTGCT ACTTCAAATT ATGCAAATTC CACTTGGGGC 
TCAGGAGGCC CCACCAGCAG CGGCCCCACC ACAAACCCTC CCCACGCCTG GGACAAAGTG 
ATTGTAGACG GGTCGGACAT GGAAGAGTGG CCTTGCATAG CCGGCGTGGA CGCAGAGCCC 
CCTTCCGACA ACGCCACAGA CAACAACAGT GCCTCGAACT CAGCCTCGGA GAAGGGACCC 
CCACAAGGAA GCACCACTAG CCACAAAGGG CGAGGGGGCC AGGGCCAGCA GCAACAGCAG 
CAGCAGCCTG GTAGTGAATG TATCCAGGGC GTCTGGAAAT CTGACCCCAA GGCTAAGCCC 
GTCCCGTCTT CCAACCCTGC TTCAGAGGGT GTAAATAGAC TGGGAAACTG GAGAAATGGG 
GGTGGGTCAG AGAGAGCAGG TTCTGCCTCT GGTTTCAGCA ACTTTAATCC AAATACTAAC 
CCATCTGCTT GGCCTGCCCT TGTCCAGGAA GGCAACAGGA AAGGGAACCC AGAGTCAGAC 
AGCAGCTTTT CTGGCGCACA ACTCGGCCCA GTGGGTTTGA CCTCTCGGGA GCAGCAGTCA 
AAGATGGAAA ATGCAGGTTC CAGCTTTGCA GGATCGGGCA GGGAGTCTGC CACAACCCAT 
CACACTGACG GACCAAAAAA TGGAAACACT AACTCTACGA ACTCAAATAC TTTGAACCCT 
CCTGATAACA AGGGAACAGC TTTTGGGGTG GGCTCAATGG ATTCCTGCCG GGGTTCAGAC 
TCCCCTGCTC AAAGCACTGG AGAAAGAAAG AATGGGGGGA TTGGCTCTTG GGGAGCGGGA 
AGGGGTCAGT CTGCCACAGG AGATGCTAAT TGTGGACATA GCAACTCTGG GAGCAATGGA 
CGAGAGAGAG AAGACGCAAG GAAAGCGCCT TCATCTCATA GACCCAATGG GTCAAGGGGG 
GAATCTTGGG AAAGCAAAAG TGGGAATGGT TCTTGGGGTT ACAGCCCGCA GGCAGACCTC 
AGTGAAGTGA AATGGAGTGA AGGGAACAAA TTGGGGGCTG GAGGGGCGTC TCAGGGAGAA 
TGGAAGCAGC CAGCCGGGCA TGAGGACTTA AAGGTGGGAG AGTGGGGAGG CTTGAACCAA 
GGAAACTCTA GCACTGGAGC ATGGGACAAT CAGAAGGGCC ACTCTCTATC GGATAACCAG 
GGCAACACGT CATCAGCAGC CTGCTGGGGC CGAGCCCCCA GCTCTGCAGG AAGTGAAGCT 
GGGGGGCAAA GCACTGGAAG CAACGCTAAA GCAGGGGGGA GCGGAGGAAG CGGAGACAGC 
CTGAACTCCA GCAGTCGACG TTCACACAGA CCTGCCCTCC CAGACAGTCA GGCTGCTCTT 
CAGACTTTAC TGAATCGCAC TGATTTGGAT CCCCGGGTGC TGTCCAACTC TGGCTGGGGC 
CAAACCCAGA TCAAGCAAGA TGCATCATGG GAGAGTGAGG AAGTGCCCTC TCGAACTGAA 
GGCAAATCAG ACCGTGGGAC TGAAGGTTGG GAGAACGTGT CCCAAGCCAA AACCTCAGGG 
GGTTGGGGGG ATGCTCCCAG CCAAAGCAAT CAAAACAAGT CAGGCTGGGG GGAGCCCCCT 
GTCCCTGCAC CTTCAACCCA AGAATGGAAG GACACCAAAG CAACTGGCTG GAATGACTAT 
AAAAACAGCC CAACTGGTTG GGTTGGTGGA GGAGGTGGTG GGGTGGTTGG AGCACGACCA 
GAGGACAAGG CAGTTCCAGG TTGGAACAAT GAAAGTGGTG GGGCTGCGGA GCCTGGGTGG 
AGTACTCGCC AGTCTAATCC AGGAGGTGGC TGGTCAGCAG GAAAGAATGG TTGGGGGGGA 
AATGGTAGTG AGGACATGGA GCCTCCCAAG GCCCCTGGAG GCTGGGAAGG CTCAGGAAGC 
AAATCCTCCT CTGGCTGGGG TGAAGTTGGA CAAAATGATG TGGGTTCTTG GGGTAATGGG 
GCAAACTCAA ATCCAGCTCC ACGAACTGGC TGGGAGGAGA CTAAGAGGCC CCAGGGAGGA 
GGAAACTGGG GTGAGGCAGC TAGGCCCCAA CTACCCTGGA ACAAACAGCA GGAATCAACG 
CCATCTTCAG CAACTCCTAG CACCTGGGGG GGCTCTCAGG CTCCCCCGCC ACCTGGAAAT 
GGGAGGTCAC AGCCAACTTC AGGGTGGAAG GGTGGTCCCC AGCCAACTGT TTCCAAGGAG 
GAAGAGCCAA GTGGCTGGGA GGAACCATCC TCACAATCCA TTGGTCATAA AATGGACATT 
GATGACGGAA CAGCAGCTTG GGGGGATCCT AACAATTACA ATTACAAAAA TGTCAGCCTG 
TGGGACAAGA ATGCTCAAGC AGGCCCTGGG CAGATGGCTC GGGAGCAAGG AATATCTGGG 
ACATTGGCAA ACAAGGCAGC TCCCTCAGTG TGGAGTAAAA ACAAAGGACC CCCAACTGAC 
AATGGGACAT CAGCTTGGGG TGAGCCAACT GAACCTGCTT CAGGGTGGGG AGAAGGTGAG 
GAGTCGGGTC CACCAACACC AGGATGGGGA AGTGCACCCC AAGTAAATCC CAGTGCCATG 
AAATCTGGTA CAAAATCTAT GCAAGATGGC TGGGGTGAGA GTGATGGGCC GGTGACAGGA 
ACACGTCACA GCAGCTGGGA TGAAGAAGAA GAGGGTGGAG TCTGGAACAG TGCAGGTTCT 
CAGGGAAGTG GCTCCTCCCA CAACTCTGCA GGTTGGGGAC AAGGAGGAAA GAAACCACAG 
ATCAAGGGTC CCTTAAAAGG AGGGAGCAAT GATACCTGGA TGAACACCTT GCCCAAACAG 
TTCTCCAACA TGGGATTGTT GAGCCAAGTT GATGATCCCT CTACCACTAA GATGGACCAT 
CCTGGCGGGC TTCCAGATAA GAAGTTTGAG ACAGACAAGC GGGGCGTGAA TCTCGGGGAT 
TTTAATGACA TGATGCGGAA GGAACGGTCT GGCTTCCGTC TGAACAATTC CAAAGAGATG 
GGAGCTGCAG ACAGTGGGCC TTATTTTGAG AAGCTGACTT TGCCTTTCTC CAATCAAGAC 
GGGTGCCTTG GGGATGAGGC TCCCTGTTCC CCTTTTTCTC CCTCCCCTAG CTACAAGCTA 
TCTCCCTCCG GCTCCTCGCT CCCTTCCGTG GGCCTGGGGG CTATCGGTTC TGGCCTCAAC 
CCCCAAACCT TCGCTGCTAG ACAAGGTGGC AATCACGGAC TATTTGGAAA CAGCGGTGCA 
CAATCGCGAG GCATGCACAA CCCTGTGCCA TCGCTCAACT CCTCTCCCAA CCTCCGGGCA 
CAAGTGCCTC CCCAGTTCCT CTCTCCCCAG GTCTCAGCCT CCATGCTAAA GCAGTTTCCT 
AACAGTAACA TGAATCCAGG ACTTTTCAAT ATGGGGCCAC AGCTTTCCCC TCAGCAGATT 
GCAATGCTGA GCCAGCTTCC TCAAATTCAG CAGTTCCAGC TGGCATGTCA GCTCCTCTTG 
CAACAACAAC AACAGCAGCA ACAGCAGCAG CAGCAGCAGC AACTCCTTCA GAACCAGAGG 
AAGATCTCAC AAGCGGTCAG ACAGCAGCAG GAACAGCAGC AGATAGCACG CATGGTAAGT 
GCCCTACAAC AGCAGCAACA GCAACAGCAG CAGCAGCAAC AGAGGCAGCC AGGAATGAAG 
CACTCTCCCT CTCACCCTGC TGGACCCAAA GGTCACTTAG ATGCTGTTGG ACTCTCAGAC 
ATGCAAGCCA AAGGGCACAT GCCATATGGA TCTGGTTTTG GCTCAAGTGG GATGGATTAT 
GGTACAGTAG GTAAGGAGGC GGGAGCAGAG TCTCGATTTA AACAGTGGAC ATCCATGATG 
GAGGGGCTAC CGTCCACTGT TTCTCAAGAT ACCAGCCTGC ATAAAAATGG CTCCATTGCA 
CCCCCAGTAA AAAATCGTGG AGGTTCCCCC TACCACCAAT ATGACATGTT GTCTACGGAT 
GCTTTGGGAG GGCATGGGGG GCCAACAGCT GAGAGCTGGT TACCTGCCAA ATCACCCCCT 
TCAAGCAAAA TTGGAAACAA GTCAATCAAT GCCAGCTGGC CCCCAGAGTT TCAGCCAGGA 
GTGCCATGGA AAGGGATCCA AAATATTGAT CCAGAATCTG ATCCTTATGT TACCCCTGGC 
AGTGTGCTTG GGGGTCCAGC CCCATCTCCC ATTGTAGATA CTGACCACCA GCTACTGCAG 
GACACCCCCA CAGGGTCCAA TTCTTCCCTC AACACCTCGC TGCCTTCACC TGGTGCCTGG 
CCCTTTAGTG CCTCTGAGAA TATCCATAGC ACTTCAGCAC TCTCTGCAAA GTTTGGGGAC 
TACAAATCGA CCTGGTCACC AGACCCCATA GGACATAACC CATCACATCA CTCAAACAAG 
ATGTGGAAAA ACCAGATGTC CAGGAACAGC CCTGGGCTGC CTCGCCCACC TCCAGGGCTC 
ACAAACAACC CCAAAGCCAC CAACTCTCCC TGGAACAGCA CAGCACCTCG CTCCGTCAGG 
GGCTGGGGCG GACAGGACTC CAGAATGGCT TCTGCATCAG CTTGGAGTGA TGGTGGATCT 
GTGCGCCCCA GCTACTGGCT GATCCTTCTT AATCTCACAC CACAGATCGA TGGTTCCACC 
CTGCGAACGA TCTGCATGCA GCATGGCCCC CTGCTGACAT TCCATCTCAA CCTTACCCAG 
GGCACAGCTC TAATCCGATA CAGCACCAAA CAGGAGGCAG CCAAGGCCCA GAGTGCCCTC 
CATATGTGTG TTCTGGGTAA TACAACTATC CTGGCGGAGT TTGCAACGGA TGAGGAGGTG 
AGCCGGTATC TTGCACAAGC TCAGCCACCA GCTCCCTCTG CTCCAGGAGC AGGCTGGCAG 
CCTTTGGAAG TGGGACCGAA CCACCCAGCT GAACCAGTAG GGCCAGCCCT AAACCTTTTT 
GGAGGAACCA CAGGGCTAGG GCAATGGAGC AGCGGCGGTG GTGGTAGTGA CCTGTCAGGG 
GTTTCTCTCT GGGGTACTCC TAGCAGCTAC ACATCCAGTC TGTGGGGTGT ACCGAGCACA 
GAGGACGCCC ACAGGATGGG CAGCCCTGCC CCGTTACTAC CCGGTGACCT TCTGGGAGGA 
GGGACCGACT CGATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756940.1
CDS	53..5830
Translation	MQNGCQSLVYAHFPLKRRKQKYINKCVWNLLLCIAFLPIFILCTCVHFFCTGFTLYASNRWIIAQLHFIREKEQEREEQLMEDKKRKKEDKKKKEAAQKVTEQKTKVPEVTKPSSSQATAASLPGSSPSPPVNGGNNAKRVAVPNGQPPSTARYMPREVPPRFRCQQDHKVLLKRGQPPPASCMLPGGGAGTPPSTTAPGAGPGNAQPGAGTLLPGESGASTDSSSGAAATSNYANSTWGSGGPTSSGPTTNPPHAWDKVIVDGSDMEEWPCIAGVDAEPPSDNATDNNSASNSASEKGPPQGSTTSHKGRGGQGQQQQQQQPGSECIQGVWKSDPKAKPVPSSNPASEGVNRLGNWRNGGGSERAGSASGFSNFNPNTNPSAWPALVQEGNRKGNPESDSSFSGAQLGPVGLTSREQQSKMENAGSSFAGSGRESATTHHTDGPKNGNTNSTNSNTLNPPDNKGTAFGVGSMDSCRGSDSPAQSTGERKNGGIGSWGAGRGQSATGDANCGHSNSGSNGREREDARKAPSSHRPNGSRGESWESKSGNGSWGYSPQADLSEVKWSEGNKLGAGGASQGEWKQPAGHEDLKVGEWGGLNQGNSSTGAWDNQKGHSLSDNQGNTSSAACWGRAPSSAGSEAGGQSTGSNAKAGGSGGSGDSLNSSSRRSHRPALPDSQAALQTLLNRTDLDPRVLSNSGWGQTQIKQDASWESEEVPSRTEGKSDRGTEGWENVSQAKTSGGWGDAPSQSNQNKSGWGEPPVPAPSTQEWKDTKATGWNDYKNSPTGWVGGGGGGVVGARPEDKAVPGWNNESGGAAEPGWSTRQSNPGGGWSAGKNGWGGNGSEDMEPPKAPGGWEGSGSKSSSGWGEVGQNDVGSWGNGANSNPAPRTGWEETKRPQGGGNWGEAARPQLPWNKQQESTPSSATPSTWGGSQAPPPPGNGRSQPTSGWKGGPQPTVSKEEEPSGWEEPSSQSIGHKMDIDDGTAAWGDPNNYNYKNVSLWDKNAQAGPGQMAREQGISGTLANKAAPSVWSKNKGPPTDNGTSAWGEPTEPASGWGEGEESGPPTPGWGSAPQVNPSAMKSGTKSMQDGWGESDGPVTGTRHSSWDEEEEGGVWNSAGSQGSGSSHNSAGWGQGGKKPQIKGPLKGGSNDTWMNTLPKQFSNMGLLSQVDDPSTTKMDHPGGLPDKKFETDKRGVNLGDFNDMMRKERSGFRLNNSKEMGAADSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSSLPSVGLGAIGSGLNPQTFAARQGGNHGLFGNSGAQSRGMHNPVPSLNSSPNLRAQVPPQFLSPQVSASMLKQFPNSNMNPGLFNMGPQLSPQQIAMLSQLPQIQQFQLACQLLLQQQQQQQQQQQQQQLLQNQRKISQAVRQQQEQQQIARMVSALQQQQQQQQQQQQRQPGMKHSPSHPAGPKGHLDAVGLSDMQAKGHMPYGSGFGSSGMDYGTVGKEAGAESRFKQWTSMMEGLPSTVSQDTSLHKNGSIAPPVKNRGGSPYHQYDMLSTDALGGHGGPTAESWLPAKSPPSSKIGNKSINASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGPAPSPIVDTDHQLLQDTPTGSNSSLNTSLPSPGAWPFSASENIHSTSALSAKFGDYKSTWSPDPIGHNPSHHSNKMWKNQMSRNSPGLPRPPPGLTNNPKATNSPWNSTAPRSVRGWGGQDSRMASASAWSDGGSVRPSYWLILLNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQSALHMCVLGNTTILAEFATDEEVSRYLAQAQPPAPSAPGAGWQPLEVGPNHPAEPVGPALNLFGGTTGLGQWSSGGGGSDLSGVSLWGTPSSYTSSLWGVPSTEDAHRMGSPAPLLPGDLLGGGTDSI

Target ORF information:

RefSeq Version	XM_031901080.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901080.1

ORF Insert Sequence:

ATGCAAAATG GCTGCCAGTC TCTCGTTTAT GCACATTTCC CTTTAAAGAG GAGAAAACAG 
AAATATATAA ACAAGTGTGT TTGGAATTTA TTATTGTGTA TTGCATTTTT GCCTATTTTT 
ATTTTGTGTA CTTGCGTTCA TTTTTTTTGT ACTGGATTTA CCTTGTATGC CTCAAACCGC 
TGGATTATAG CACAGTTGCA CTTTATAAGA GAGAAGGAGC AAGAAAGGGA AGAACAGTTA 
ATGGAAGACA AGAAAAGGAA GAAAGAGGAT AAAAAGAAGA AAGAAGCTGC TCAGAAGGTC 
ACAGAACAAA AAACCAAAGT GCCCGAAGTG ACGAAACCAA GTTCAAGCCA AGCGACGGCC 
GCCAGCCTAC CTGGCAGCTC CCCCTCGCCA CCAGTCAATG GTGGCAACAA TGCCAAAAGG 
GTGGCAGTGC CGAACGGACA ACCGCCCAGC ACCGCCCGCT ATATGCCTCG GGAGGTGCCG 
CCGCGATTCC GCTGCCAGCA GGACCACAAA GTGTTACTGA AGCGTGGACA GCCTCCTCCA 
GCGTCCTGTA TGCTGCCGGG GGGAGGAGCA GGGACTCCCC CCTCAACCAC TGCTCCAGGG 
GCCGGCCCAG GCAACGCACA GCCAGGGGCG GGGACACTGC TGCCAGGGGA AAGCGGGGCT 
TCAACAGACT CATCATCGGG GGCAGCTGCT ACTTCAAATT ATGCAAATTC CACTTGGGGC 
TCAGGAGGCC CCACCAGCAG CGGCCCCACC ACAAACCCTC CCCACGCCTG GGACAAAGTG 
ATTGTAGACG GGTCGGACAT GGAAGAGTGG CCTTGCATAG CCGGCGTGGA CGCAGAGCCC 
CCTTCCGACA ACGCCACAGA CAACAACAGT GCCTCGAACT CAGCCTCGGA GAAGGGACCC 
CCACAAGGAA GCACCACTAG CCACAAAGGG CGAGGGGGCC AGGGCCAGCA GCAACAGCAG 
CAGCAGCCTG GTAGTGAATG TATCCAGGGC GTCTGGAAAT CTGACCCCAA GGCTAAGCCC 
GTCCCGTCTT CCAACCCTGC TTCAGAGGGT GTAAATAGAC TGGGAAACTG GAGAAATGGG 
GGTGGGTCAG AGAGAGCAGG TTCTGCCTCT GGTTTCAGCA ACTTTAATCC AAATACTAAC 
CCATCTGCTT GGCCTGCCCT TGTCCAGGAA GGCAACAGGA AAGGGAACCC AGAGTCAGAC 
AGCAGCTTTT CTGGCGCACA ACTCGGCCCA GTGGGTTTGA CCTCTCGGGA GCAGCAGTCA 
AAGATGGAAA ATGCAGGTTC CAGCTTTGCA GGATCGGGCA GGGAGTCTGC CACAACCCAT 
CACACTGACG GACCAAAAAA TGGAAACACT AACTCTACGA ACTCAAATAC TTTGAACCCT 
CCTGATAACA AGGGAACAGC TTTTGGGGTG GGCTCAATGG ATTCCTGCCG GGGTTCAGAC 
TCCCCTGCTC AAAGCACTGG AGAAAGAAAG AATGGGGGGA TTGGCTCTTG GGGAGCGGGA 
AGGGGTCAGT CTGCCACAGG AGATGCTAAT TGTGGACATA GCAACTCTGG GAGCAATGGA 
CGAGAGAGAG AAGACGCAAG GAAAGCGCCT TCATCTCATA GACCCAATGG GTCAAGGGGG 
GAATCTTGGG AAAGCAAAAG TGGGAATGGT TCTTGGGGTT ACAGCCCGCA GGCAGACCTC 
AGTGAAGTGA AATGGAGTGA AGGGAACAAA TTGGGGGCTG GAGGGGCGTC TCAGGGAGAA 
TGGAAGCAGC CAGCCGGGCA TGAGGACTTA AAGGTGGGAG AGTGGGGAGG CTTGAACCAA 
GGAAACTCTA GCACTGGAGC ATGGGACAAT CAGAAGGGCC ACTCTCTATC GGATAACCAG 
GGCAACACGT CATCAGCAGC CTGCTGGGGC CGAGCCCCCA GCTCTGCAGG AAGTGAAGCT 
GGGGGGCAAA GCACTGGAAG CAACGCTAAA GCAGGGGGGA GCGGAGGAAG CGGAGACAGC 
CTGAACTCCA GCAGTCGACG TTCACACAGA CCTGCCCTCC CAGACAGTCA GGCTGCTCTT 
CAGACTTTAC TGAATCGCAC TGATTTGGAT CCCCGGGTGC TGTCCAACTC TGGCTGGGGC 
CAAACCCAGA TCAAGCAAGA TGCATCATGG GAGAGTGAGG AAGTGCCCTC TCGAACTGAA 
GGCAAATCAG ACCGTGGGAC TGAAGGTTGG GAGAACGTGT CCCAAGCCAA AACCTCAGGG 
GGTTGGGGGG ATGCTCCCAG CCAAAGCAAT CAAAACAAGT CAGGCTGGGG GGAGCCCCCT 
GTCCCTGCAC CTTCAACCCA AGAATGGAAG GACACCAAAG CAACTGGCTG GAATGACTAT 
AAAAACAGCC CAACTGGTTG GGTTGGTGGA GGAGGTGGTG GGGTGGTTGG AGCACGACCA 
GAGGACAAGG CAGTTCCAGG TTGGAACAAT GAAAGTGGTG GGGCTGCGGA GCCTGGGTGG 
AGTACTCGCC AGTCTAATCC AGGAGGTGGC TGGTCAGCAG GAAAGAATGG TTGGGGGGGA 
AATGGTAGTG AGGACATGGA GCCTCCCAAG GCCCCTGGAG GCTGGGAAGG CTCAGGAAGC 
AAATCCTCCT CTGGCTGGGG TGAAGTTGGA CAAAATGATG TGGGTTCTTG GGGTAATGGG 
GCAAACTCAA ATCCAGCTCC ACGAACTGGC TGGGAGGAGA CTAAGAGGCC CCAGGGAGGA 
GGAAACTGGG GTGAGGCAGC TAGGCCCCAA CTACCCTGGA ACAAACAGCA GGAATCAACG 
CCATCTTCAG CAACTCCTAG CACCTGGGGG GGCTCTCAGG CTCCCCCGCC ACCTGGAAAT 
GGGAGGTCAC AGCCAACTTC AGGGTGGAAG GGTGGTCCCC AGCCAACTGT TTCCAAGGAG 
GAAGAGCCAA GTGGCTGGGA GGAACCATCC TCACAATCCA TTGGTCATAA AATGGACATT 
GATGACGGAA CAGCAGCTTG GGGGGATCCT AACAATTACA ATTACAAAAA TGTCAGCCTG 
TGGGACAAGA ATGCTCAAGC AGGCCCTGGG CAGATGGCTC GGGAGCAAGG AATATCTGGG 
ACATTGGCAA ACAAGGCAGC TCCCTCAGTG TGGAGTAAAA ACAAAGGACC CCCAACTGAC 
AATGGGACAT CAGCTTGGGG TGAGCCAACT GAACCTGCTT CAGGGTGGGG AGAAGGTGAG 
GAGTCGGGTC CACCAACACC AGGATGGGGA AGTGCACCCC AAGTAAATCC CAGTGCCATG 
AAATCTGGTA CAAAATCTAT GCAAGATGGC TGGGGTGAGA GTGATGGGCC GGTGACAGGA 
ACACGTCACA GCAGCTGGGA TGAAGAAGAA GAGGGTGGAG TCTGGAACAG TGCAGGTTCT 
CAGGGAAGTG GCTCCTCCCA CAACTCTGCA GGTTGGGGAC AAGGAGGAAA GAAACCACAG 
ATCAAGGGTC CCTTAAAAGG AGGGAGCAAT GATACCTGGA TGAACACCTT GCCCAAACAG 
TTCTCCAACA TGGGATTGTT GAGCCAAGTT GATGATCCCT CTACCACTAA GATGGACCAT 
CCTGGCGGGC TTCCAGATAA GAAGTTTGAG ACAGACAAGC GGGGCGTGAA TCTCGGGGAT 
TTTAATGACA TGATGCGGAA GGAACGGTCT GGCTTCCGTC TGAACAATTC CAAAGAGATG 
GGAGCTGCAG ACAGTGGGCC TTATTTTGAG AAGCTGACTT TGCCTTTCTC CAATCAAGAC 
GGGTGCCTTG GGGATGAGGC TCCCTGTTCC CCTTTTTCTC CCTCCCCTAG CTACAAGCTA 
TCTCCCTCCG GCTCCTCGCT CCCTTCCGTG GGCCTGGGGG CTATCGGTTC TGGCCTCAAC 
CCCCAAACCT TCGCTGCTAG ACAAGGTGGC AATCACGGAC TATTTGGAAA CAGCGGTGCA 
CAATCGCGAG GCATGCACAA CCCTGTGCCA TCGCTCAACT CCTCTCCCAA CCTCCGGGCA 
CAAGTGCCTC CCCAGTTCCT CTCTCCCCAG GTCTCAGCCT CCATGCTAAA GCAGTTTCCT 
AACAGTAACA TGAATCCAGG ACTTTTCAAT ATGGGGCCAC AGCTTTCCCC TCAGCAGATT 
GCAATGCTGA GCCAGCTTCC TCAAATTCAG CAGTTCCAGC TGGCATGTCA GCTCCTCTTG 
CAACAACAAC AACAGCAGCA ACAGCAGCAG CAGCAGCAGC AACTCCTTCA GAACCAGAGG 
AAGATCTCAC AAGCGGTCAG ACAGCAGCAG GAACAGCAGC AGATAGCACG CATGGTAAGT 
GCCCTACAAC AGCAGCAACA GCAACAGCAG CAGCAGCAAC AGAGGCAGCC AGGAATGAAG 
CACTCTCCCT CTCACCCTGC TGGACCCAAA GGTCACTTAG ATGCTGTTGG ACTCTCAGAC 
ATGCAAGCCA AAGGGCACAT GCCATATGGA TCTGGTTTTG GCTCAAGTGG GATGGATTAT 
GGTACAGTAG GTAAGGAGGC GGGAGCAGAG TCTCGATTTA AACAGTGGAC ATCCATGATG 
GAGGGGCTAC CGTCCACTGT TTCTCAAGAT ACCAGCCTGC ATAAAAATGG CTCCATTGCA 
CCCCCAGTAA AAAATCGTGG AGGTTCCCCC TACCACCAAT ATGACATGTT GTCTACGGAT 
GCTTTGGGAG GGCATGGGGG GCCAACAGCT GAGAGCTGGT TACCTGCCAA ATCACCCCCT 
TCAAGCAAAA TTGGAAACAA GTCAATCAAT GCCAGCTGGC CCCCAGAGTT TCAGCCAGGA 
GTGCCATGGA AAGGGATCCA AAATATTGAT CCAGAATCTG ATCCTTATGT TACCCCTGGC 
AGTGTGCTTG GGGGTCCAGC CCCATCTCCC ATTGTAGATA CTGACCACCA GCTACTGCAG 
GACACCCCCA CAGGGTCCAA TTCTTCCCTC AACACCTCGC TGCCTTCACC TGGTGCCTGG 
CCCTTTAGTG CCTCTGAGAA TATCCATAGC ACTTCAGCAC TCTCTGCAAA GTTTGGGGAC 
TACAAATCGA CCTGGTCACC AGACCCCATA GGACATAACC CATCACATCA CTCAAACAAG 
ATGTGGAAAA ACCAGATGTC CAGGAACAGC CCTGGGCTGC CTCGCCCACC TCCAGGGCTC 
ACAAACAACC CCAAAGCCAC CAACTCTCCC TGGAACAGCA CAGCACCTCG CTCCGTCAGG 
GGCTGGGGCG GACAGGACTC CAGAATGGCT TCTGCATCAG CTTGGAGTGA TGGTGGATCT 
GTGCGCCCCA GCTACTGGCT GATCCTTCTT AATCTCACAC CACAGATCGA TGGTTCCACC 
CTGCGAACGA TCTGCATGCA GCATGGCCCC CTGCTGACAT TCCATCTCAA CCTTACCCAG 
GGCACAGCTC TAATCCGATA CAGCACCAAA CAGGAGGCAG CCAAGGCCCA GAGTGCCCTC 
CATATGTGTG TTCTGGGTAA TACAACTATC CTGGCGGAGT TTGCAACGGA TGAGGAGGTG 
AGCCGGTATC TTGCACAAGC TCAGCCACCA GCTCCCTCTG CTCCAGGAGC AGGCTGGCAG 
CCTTTGGAAG TGGGACCGAA CCACCCAGCT GAACCAGTAG GGCCAGCCCT AAACCTTTTT 
GGAGGAACCA CAGGGCTAGG GCAATGGAGC AGCGGCGGTG GTGGTAGTGA CCTGTCAGGG 
GTTTCTCTCT GGGGTACTCC TAGCAGCTAC ACATCCAGTC TGTGGGGTGT ACCGAGCACA 
GAGGACGCCC ACAGGATGGG CAGCCCTGCC CCGTTACTAC CCGGTGACCT TCTGGGAGGA 
GGGACCGACT CGATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnrc6b ( XM_031901081.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756941.1 Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X2, mRNA.

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CloneID	OXa57433
Clone ID Related Accession (Same CDS sequence)	XM_031901081.1
Accession Version	XM_031901081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trinucleotide repeat-containing gene 6B protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAATG GCTGCCAGTC TCTCGTTTAT GCACATTTCC CTTTAAAGAG GAGAAAACAG 
AAATATATAA ACAAGTGTGT TTGGAATTTA TTATTGTGTA TTGCATTTTT GCCTATTTTT 
ATTTTGTGTA CTTGCGTTCA TTTTTTTTGT ACTGGATTTA CCTTGTATGC CTCAAACCGC 
TGGATTATAG CACAGTTGCA CTTTATAAGA GAGAAGGAGC AAGAAAGGGA AGAACAGTTA 
ATGGAAGACA AGAAAAGGAA GAAAGAGGAT AAAAAGAAGA AAGAAGCTGC TCAGAAGGTC 
ACAGAACAAA AAACCAAAGT GCCCGAAGTG ACGAAACCAA GTTCAAGCCA AGCGACGGCC 
GCCAGCCTAC CTGGCAGCTC CCCCTCGCCA CCAGTCAATG GTGGCAACAA TGCCAAAAGG 
GTGGCAGTGC CGAACGGACA ACCGCCCAGC ACCGCCCGCT ATATGCCTCG GGAGGTGCCG 
CCGCGATTCC GCTGCCAGCA GGACCACAAA GTGTTACTGA AGCGTGGACA GCCTCCTCCA 
GCGTCCTGTA TGCTGCCGGG GGGAGGAGCA GGGACTCCCC CCTCAACCAC TGCTCCAGGG 
GCCGGCCCAG GCAACGCACA GCCAGGGGCG GGGACACTGC TGCCAGGGGA AAGCGGGGCT 
TCAACAGACT CATCATCGGG GGCAGCTGCT ACTTCAAATT ATGCAAATTC CACTTGGGGC 
TCAGGAGGCC CCACCAGCAG CGGCCCCACC ACAAACCCTC CCCACGCCTG GGACAAAGTG 
ATTGTAGACG GGTCGGACAT GGAAGAGTGG CCTTGCATAG CCGGCGTGGA CGCAGAGCCC 
CCTTCCGACA ACGCCACAGA CAACAACAGT GCCTCGAACT CAGCCTCGGA GAAGGGACCC 
CCACAAGGAA GCACCACTAG CCACAAAGGG CGAGGGGGCC AGGGCCAGCA GCAACAGCAG 
CAGCAGCCTG GTAGTGAATG TATCCAGGGC GTCTGGAAAT CTGACCCCAA GGCTAAGCCC 
GTCCCGTCTT CCAACCCTGC TTCAGAGGGT GTAAATAGAC TGGGAAACTG GAGAAATGGG 
GGTGGGTCAG AGAGAGCAGG TTCTGCCTCT GGTTTCAGCA ACTTTAATCC AAATACTAAC 
CCATCTGCTT GGCCTGCCCT TGTCCAGGAA GGCAACAGGA AAGGGAACCC AGAGTCAGAC 
AGCAGCTTTT CTGGCGCACA ACTCGGCCCA GTGGGTTTGA CCTCTCGGGA GCAGCAGTCA 
AAGATGGAAA ATGCAGGTTC CAGCTTTGCA GGATCGGGCA GGGAGTCTGC CACAACCCAT 
CACACTGACG GACCAAAAAA TGGAAACACT AACTCTACGA ACTCAAATAC TTTGAACCCT 
CCTGATAACA AGGGAACAGC TTTTGGGGTG GGCTCAATGG ATTCCTGCCG GGGTTCAGAC 
TCCCCTGCTC AAAGCACTGG AGAAAGAAAG AATGGGGGGA TTGGCTCTTG GGGAGCGGGA 
AGGGGTCAGT CTGCCACAGG AGATGCTAAT TGTGGACATA GCAACTCTGG GAGCAATGGA 
CGAGAGAGAG AAGACGCAAG GAAAGCGCCT TCATCTCATA GACCCAATGG GTCAAGGGGG 
GAATCTTGGG AAAGCAAAAG TGGGAATGGT TCTTGGGGTT ACAGCCCGCA GGCAGACCTC 
AGTGAAGTGA AATGGAGTGA AGGGAACAAA TTGGGGGCTG GAGGGGCGTC TCAGGGAGAA 
TGGAAGCAGC CAGCCGGGCA TGAGGACTTA AAGGTGGGAG AGTGGGGAGG CTTGAACCAA 
GGAAACTCTA GCACTGGAGC ATGGGACAAT CAGAAGGGCC ACTCTCTATC GGATAACCAG 
GGCAACACGT CATCAGCAGC CTGCTGGGGC CGAGCCCCCA GCTCTGCAGG AAGTGAAGCT 
GGGGGGCAAA GCACTGGAAG CAACGCTAAA GCAGGGGGGA GCGGAGGAAG CGGAGACAGC 
CTGAACTCCA GCAGTCGACG TTCACACAGA CCTGCCCTCC CAGACAGTCA GGCTGCTCTT 
CAGACTTTAC TGAATCGCAC TGATTTGGAT CCCCGGGTGC TGTCCAACTC TGGCTGGGGC 
CAAACCCAGA TCAAGCAAGA TGCATCATGG GAGAGTGAGG AAGTGCCCTC TCGAACTGAA 
GGCAAATCAG ACCGTGGGAC TGAAGGTTGG GAGAACGTGT CCCAAGCCAA AACCTCAGGG 
GGTTGGGGGG ATGCTCCCAG CCAAAGCAAT CAAAACAAGT CAGGCTGGGG GGAGCCCCCT 
GTCCCTGCAC CTTCAACCCA AGAATGGAAG GACACCAAAG CAACTGGCTG GAATGACTAT 
AAAAACAGCC CAACTGGTTG GGTTGGTGGA GGAGGTGGTG GGGTGGTTGG AGCACGACCA 
GAGGACAAGG CAGTTCCAGG TTGGAACAAT GAAAGTGGTG GGGCTGCGGA GCCTGGGTGG 
AGTACTCGCC AGTCTAATCC AGGAGGTGGC TGGTCAGCAG GAAAGAATGG TTGGGGGGGA 
AATGGTAGTG AGGACATGGA GCCTCCCAAG GCCCCTGGAG GCTGGGAAGG CTCAGGAAGC 
AAATCCTCCT CTGGCTGGGG TGAAGTTGGA CAAAATGATG TGGGTTCTTG GGGTAATGGG 
GCAAACTCAA ATCCAGCTCC ACGAACTGGC TGGGAGGAGA CTAAGAGGCC CCAGGGAGGA 
GGAAACTGGG GTGAGGCAGC TAGGCCCCAA CTACCCTGGA ACAAACAGCA GGAATCAACG 
CCATCTTCAG CAACTCCTAG CACCTGGGGG GGCTCTCAGG CTCCCCCGCC ACCTGGAAAT 
GGGAGGTCAC AGCCAACTTC AGGGTGGAAG GGTGGTCCCC AGCCAACTGT TTCCAAGGAG 
GAAGAGCCAA GTGGCTGGGA GGAACCATCC TCACAATCCA TTGGTCATAA AATGGACATT 
GATGACGGAA CAGCAGCTTG GGGGGATCCT AACAATTACA ATTACAAAAA TGTCAGCCTG 
TGGGACAAGA ATGCTCAAGC AGGCCCTGGG CAGATGGCTC GGGAGCAAGG AATATCTGGG 
ACATTGGCAA ACAAGGCAGC TCCCTCAGTG TGGAGTAAAA ACAAAGGACC CCCAACTGAC 
AATGGGACAT CAGCTTGGGG TGAGCCAACT GAACCTGCTT CAGGGTGGGG AGAAGGTGAG 
GAGTCGGGTC CACCAACACC AGGATGGGGA AGTGCACCCC AAGTAAATCC CAGTGCCATG 
AAATCTGGTA CAAAATCTAT GCAAGATGGC TGGGGTGAGA GTGATGGGCC GGTGACAGGA 
ACACGTCACA GCAGCTGGGA TGAAGAAGAA GAGGGTGGAG TCTGGAACAG TGCAGGTTCT 
CAGGGAAGTG GCTCCTCCCA CAACTCTGCA GGTTGGGGAC AAGGAGGAAA GAAACCACAG 
ATCAAGGGTC CCTTAAAAGG AGGGAGCAAT GATACCTGGA TGAACACCTT GCCCAAACAG 
TTCTCCAACA TGGGATTGTT GAGCCAAGTT GATGATCCCT CTACCACTAA GATGGACCAT 
CCTGGCGGGC TTCCAGATAA GAAGTTTGAG ACAGACAAGC GGGGCGTGAA TCTCGGGGAT 
TTTAATGACA TGATGCGGAA GGAACGGTCT GGCTTCCGTC TGAACAATTC CAAAGAGATG 
GGAGCTGCAG ACAGTGGGCC TTATTTTGAG AAGCTGACTT TGCCTTTCTC CAATCAAGAC 
GGGTGCCTTG GGGATGAGGC TCCCTGTTCC CCTTTTTCTC CCTCCCCTAG CTACAAGCTA 
TCTCCCTCCG GCTCCTCGCT CCCTTCCGTG GGCCTGGGGG CTATCGGTTC TGGCCTCAAC 
CCCCAAACCT TCGCTGCTAG ACAAGGTGGC AATCACGGAC TATTTGGAAA CAGCGGTGCA 
CAATCGCGAG GCATGCACAA CCCTGTGCCA TCGCTCAACT CCTCTCCCAA CCTCCGGGCA 
CAAGTGCCTC CCCAGTTCCT CTCTCCCCAG GTCTCAGCCT CCATGCTAAA GCAGTTTCCT 
AACAGTAACA TGAATCCAGG ACTTTTCAAT ATGGGGCCAC AGCTTTCCCC TCAGCAGATT 
GCAATGCTGA GCCAGCTTCC TCAAATTCAG CAGTTCCAGC TGGCATGTCA GCTCCTCTTG 
CAACAACAAC AACAGCAGCA ACAGCAGCAG CAGCAGCAGC AACTCCTTCA GAACCAGAGG 
AAGATCTCAC AAGCGGTCAG ACAGCAGCAG GAACAGCAGA TAGCACGCAT GGTAAGTGCC 
CTACAACAGC AGCAACAGCA ACAGCAGCAG CAGCAACAGA GGCAGCCAGG AATGAAGCAC 
TCTCCCTCTC ACCCTGCTGG ACCCAAAGGT CACTTAGATG CTGTTGGACT CTCAGACATG 
CAAGCCAAAG GGCACATGCC ATATGGATCT GGTTTTGGCT CAAGTGGGAT GGATTATGGT 
ACAGTAGGTA AGGAGGCGGG AGCAGAGTCT CGATTTAAAC AGTGGACATC CATGATGGAG 
GGGCTACCGT CCACTGTTTC TCAAGATACC AGCCTGCATA AAAATGGCTC CATTGCACCC 
CCAGTAAAAA ATCGTGGAGG TTCCCCCTAC CACCAATATG ACATGTTGTC TACGGATGCT 
TTGGGAGGGC ATGGGGGGCC AACAGCTGAG AGCTGGTTAC CTGCCAAATC ACCCCCTTCA 
AGCAAAATTG GAAACAAGTC AATCAATGCC AGCTGGCCCC CAGAGTTTCA GCCAGGAGTG 
CCATGGAAAG GGATCCAAAA TATTGATCCA GAATCTGATC CTTATGTTAC CCCTGGCAGT 
GTGCTTGGGG GTCCAGCCCC ATCTCCCATT GTAGATACTG ACCACCAGCT ACTGCAGGAC 
ACCCCCACAG GGTCCAATTC TTCCCTCAAC ACCTCGCTGC CTTCACCTGG TGCCTGGCCC 
TTTAGTGCCT CTGAGAATAT CCATAGCACT TCAGCACTCT CTGCAAAGTT TGGGGACTAC 
AAATCGACCT GGTCACCAGA CCCCATAGGA CATAACCCAT CACATCACTC AAACAAGATG 
TGGAAAAACC AGATGTCCAG GAACAGCCCT GGGCTGCCTC GCCCACCTCC AGGGCTCACA 
AACAACCCCA AAGCCACCAA CTCTCCCTGG AACAGCACAG CACCTCGCTC CGTCAGGGGC 
TGGGGCGGAC AGGACTCCAG AATGGCTTCT GCATCAGCTT GGAGTGATGG TGGATCTGTG 
CGCCCCAGCT ACTGGCTGAT CCTTCTTAAT CTCACACCAC AGATCGATGG TTCCACCCTG 
CGAACGATCT GCATGCAGCA TGGCCCCCTG CTGACATTCC ATCTCAACCT TACCCAGGGC 
ACAGCTCTAA TCCGATACAG CACCAAACAG GAGGCAGCCA AGGCCCAGAG TGCCCTCCAT 
ATGTGTGTTC TGGGTAATAC AACTATCCTG GCGGAGTTTG CAACGGATGA GGAGGTGAGC 
CGGTATCTTG CACAAGCTCA GCCACCAGCT CCCTCTGCTC CAGGAGCAGG CTGGCAGCCT 
TTGGAAGTGG GACCGAACCA CCCAGCTGAA CCAGTAGGGC CAGCCCTAAA CCTTTTTGGA 
GGAACCACAG GGCTAGGGCA ATGGAGCAGC GGCGGTGGTG GTAGTGACCT GTCAGGGGTT 
TCTCTCTGGG GTACTCCTAG CAGCTACACA TCCAGTCTGT GGGGTGTACC GAGCACAGAG 
GACGCCCACA GGATGGGCAG CCCTGCCCCG TTACTACCCG GTGACCTTCT GGGAGGAGGG 
ACCGACTCGA TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756941.1
CDS	53..5827
Translation	MQNGCQSLVYAHFPLKRRKQKYINKCVWNLLLCIAFLPIFILCTCVHFFCTGFTLYASNRWIIAQLHFIREKEQEREEQLMEDKKRKKEDKKKKEAAQKVTEQKTKVPEVTKPSSSQATAASLPGSSPSPPVNGGNNAKRVAVPNGQPPSTARYMPREVPPRFRCQQDHKVLLKRGQPPPASCMLPGGGAGTPPSTTAPGAGPGNAQPGAGTLLPGESGASTDSSSGAAATSNYANSTWGSGGPTSSGPTTNPPHAWDKVIVDGSDMEEWPCIAGVDAEPPSDNATDNNSASNSASEKGPPQGSTTSHKGRGGQGQQQQQQQPGSECIQGVWKSDPKAKPVPSSNPASEGVNRLGNWRNGGGSERAGSASGFSNFNPNTNPSAWPALVQEGNRKGNPESDSSFSGAQLGPVGLTSREQQSKMENAGSSFAGSGRESATTHHTDGPKNGNTNSTNSNTLNPPDNKGTAFGVGSMDSCRGSDSPAQSTGERKNGGIGSWGAGRGQSATGDANCGHSNSGSNGREREDARKAPSSHRPNGSRGESWESKSGNGSWGYSPQADLSEVKWSEGNKLGAGGASQGEWKQPAGHEDLKVGEWGGLNQGNSSTGAWDNQKGHSLSDNQGNTSSAACWGRAPSSAGSEAGGQSTGSNAKAGGSGGSGDSLNSSSRRSHRPALPDSQAALQTLLNRTDLDPRVLSNSGWGQTQIKQDASWESEEVPSRTEGKSDRGTEGWENVSQAKTSGGWGDAPSQSNQNKSGWGEPPVPAPSTQEWKDTKATGWNDYKNSPTGWVGGGGGGVVGARPEDKAVPGWNNESGGAAEPGWSTRQSNPGGGWSAGKNGWGGNGSEDMEPPKAPGGWEGSGSKSSSGWGEVGQNDVGSWGNGANSNPAPRTGWEETKRPQGGGNWGEAARPQLPWNKQQESTPSSATPSTWGGSQAPPPPGNGRSQPTSGWKGGPQPTVSKEEEPSGWEEPSSQSIGHKMDIDDGTAAWGDPNNYNYKNVSLWDKNAQAGPGQMAREQGISGTLANKAAPSVWSKNKGPPTDNGTSAWGEPTEPASGWGEGEESGPPTPGWGSAPQVNPSAMKSGTKSMQDGWGESDGPVTGTRHSSWDEEEEGGVWNSAGSQGSGSSHNSAGWGQGGKKPQIKGPLKGGSNDTWMNTLPKQFSNMGLLSQVDDPSTTKMDHPGGLPDKKFETDKRGVNLGDFNDMMRKERSGFRLNNSKEMGAADSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSSLPSVGLGAIGSGLNPQTFAARQGGNHGLFGNSGAQSRGMHNPVPSLNSSPNLRAQVPPQFLSPQVSASMLKQFPNSNMNPGLFNMGPQLSPQQIAMLSQLPQIQQFQLACQLLLQQQQQQQQQQQQQQLLQNQRKISQAVRQQQEQQIARMVSALQQQQQQQQQQQQRQPGMKHSPSHPAGPKGHLDAVGLSDMQAKGHMPYGSGFGSSGMDYGTVGKEAGAESRFKQWTSMMEGLPSTVSQDTSLHKNGSIAPPVKNRGGSPYHQYDMLSTDALGGHGGPTAESWLPAKSPPSSKIGNKSINASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGPAPSPIVDTDHQLLQDTPTGSNSSLNTSLPSPGAWPFSASENIHSTSALSAKFGDYKSTWSPDPIGHNPSHHSNKMWKNQMSRNSPGLPRPPPGLTNNPKATNSPWNSTAPRSVRGWGGQDSRMASASAWSDGGSVRPSYWLILLNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQSALHMCVLGNTTILAEFATDEEVSRYLAQAQPPAPSAPGAGWQPLEVGPNHPAEPVGPALNLFGGTTGLGQWSSGGGGSDLSGVSLWGTPSSYTSSLWGVPSTEDAHRMGSPAPLLPGDLLGGGTDSI

Target ORF information:

RefSeq Version	XM_031901081.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901081.1

ORF Insert Sequence:

ATGCAAAATG GCTGCCAGTC TCTCGTTTAT GCACATTTCC CTTTAAAGAG GAGAAAACAG 
AAATATATAA ACAAGTGTGT TTGGAATTTA TTATTGTGTA TTGCATTTTT GCCTATTTTT 
ATTTTGTGTA CTTGCGTTCA TTTTTTTTGT ACTGGATTTA CCTTGTATGC CTCAAACCGC 
TGGATTATAG CACAGTTGCA CTTTATAAGA GAGAAGGAGC AAGAAAGGGA AGAACAGTTA 
ATGGAAGACA AGAAAAGGAA GAAAGAGGAT AAAAAGAAGA AAGAAGCTGC TCAGAAGGTC 
ACAGAACAAA AAACCAAAGT GCCCGAAGTG ACGAAACCAA GTTCAAGCCA AGCGACGGCC 
GCCAGCCTAC CTGGCAGCTC CCCCTCGCCA CCAGTCAATG GTGGCAACAA TGCCAAAAGG 
GTGGCAGTGC CGAACGGACA ACCGCCCAGC ACCGCCCGCT ATATGCCTCG GGAGGTGCCG 
CCGCGATTCC GCTGCCAGCA GGACCACAAA GTGTTACTGA AGCGTGGACA GCCTCCTCCA 
GCGTCCTGTA TGCTGCCGGG GGGAGGAGCA GGGACTCCCC CCTCAACCAC TGCTCCAGGG 
GCCGGCCCAG GCAACGCACA GCCAGGGGCG GGGACACTGC TGCCAGGGGA AAGCGGGGCT 
TCAACAGACT CATCATCGGG GGCAGCTGCT ACTTCAAATT ATGCAAATTC CACTTGGGGC 
TCAGGAGGCC CCACCAGCAG CGGCCCCACC ACAAACCCTC CCCACGCCTG GGACAAAGTG 
ATTGTAGACG GGTCGGACAT GGAAGAGTGG CCTTGCATAG CCGGCGTGGA CGCAGAGCCC 
CCTTCCGACA ACGCCACAGA CAACAACAGT GCCTCGAACT CAGCCTCGGA GAAGGGACCC 
CCACAAGGAA GCACCACTAG CCACAAAGGG CGAGGGGGCC AGGGCCAGCA GCAACAGCAG 
CAGCAGCCTG GTAGTGAATG TATCCAGGGC GTCTGGAAAT CTGACCCCAA GGCTAAGCCC 
GTCCCGTCTT CCAACCCTGC TTCAGAGGGT GTAAATAGAC TGGGAAACTG GAGAAATGGG 
GGTGGGTCAG AGAGAGCAGG TTCTGCCTCT GGTTTCAGCA ACTTTAATCC AAATACTAAC 
CCATCTGCTT GGCCTGCCCT TGTCCAGGAA GGCAACAGGA AAGGGAACCC AGAGTCAGAC 
AGCAGCTTTT CTGGCGCACA ACTCGGCCCA GTGGGTTTGA CCTCTCGGGA GCAGCAGTCA 
AAGATGGAAA ATGCAGGTTC CAGCTTTGCA GGATCGGGCA GGGAGTCTGC CACAACCCAT 
CACACTGACG GACCAAAAAA TGGAAACACT AACTCTACGA ACTCAAATAC TTTGAACCCT 
CCTGATAACA AGGGAACAGC TTTTGGGGTG GGCTCAATGG ATTCCTGCCG GGGTTCAGAC 
TCCCCTGCTC AAAGCACTGG AGAAAGAAAG AATGGGGGGA TTGGCTCTTG GGGAGCGGGA 
AGGGGTCAGT CTGCCACAGG AGATGCTAAT TGTGGACATA GCAACTCTGG GAGCAATGGA 
CGAGAGAGAG AAGACGCAAG GAAAGCGCCT TCATCTCATA GACCCAATGG GTCAAGGGGG 
GAATCTTGGG AAAGCAAAAG TGGGAATGGT TCTTGGGGTT ACAGCCCGCA GGCAGACCTC 
AGTGAAGTGA AATGGAGTGA AGGGAACAAA TTGGGGGCTG GAGGGGCGTC TCAGGGAGAA 
TGGAAGCAGC CAGCCGGGCA TGAGGACTTA AAGGTGGGAG AGTGGGGAGG CTTGAACCAA 
GGAAACTCTA GCACTGGAGC ATGGGACAAT CAGAAGGGCC ACTCTCTATC GGATAACCAG 
GGCAACACGT CATCAGCAGC CTGCTGGGGC CGAGCCCCCA GCTCTGCAGG AAGTGAAGCT 
GGGGGGCAAA GCACTGGAAG CAACGCTAAA GCAGGGGGGA GCGGAGGAAG CGGAGACAGC 
CTGAACTCCA GCAGTCGACG TTCACACAGA CCTGCCCTCC CAGACAGTCA GGCTGCTCTT 
CAGACTTTAC TGAATCGCAC TGATTTGGAT CCCCGGGTGC TGTCCAACTC TGGCTGGGGC 
CAAACCCAGA TCAAGCAAGA TGCATCATGG GAGAGTGAGG AAGTGCCCTC TCGAACTGAA 
GGCAAATCAG ACCGTGGGAC TGAAGGTTGG GAGAACGTGT CCCAAGCCAA AACCTCAGGG 
GGTTGGGGGG ATGCTCCCAG CCAAAGCAAT CAAAACAAGT CAGGCTGGGG GGAGCCCCCT 
GTCCCTGCAC CTTCAACCCA AGAATGGAAG GACACCAAAG CAACTGGCTG GAATGACTAT 
AAAAACAGCC CAACTGGTTG GGTTGGTGGA GGAGGTGGTG GGGTGGTTGG AGCACGACCA 
GAGGACAAGG CAGTTCCAGG TTGGAACAAT GAAAGTGGTG GGGCTGCGGA GCCTGGGTGG 
AGTACTCGCC AGTCTAATCC AGGAGGTGGC TGGTCAGCAG GAAAGAATGG TTGGGGGGGA 
AATGGTAGTG AGGACATGGA GCCTCCCAAG GCCCCTGGAG GCTGGGAAGG CTCAGGAAGC 
AAATCCTCCT CTGGCTGGGG TGAAGTTGGA CAAAATGATG TGGGTTCTTG GGGTAATGGG 
GCAAACTCAA ATCCAGCTCC ACGAACTGGC TGGGAGGAGA CTAAGAGGCC CCAGGGAGGA 
GGAAACTGGG GTGAGGCAGC TAGGCCCCAA CTACCCTGGA ACAAACAGCA GGAATCAACG 
CCATCTTCAG CAACTCCTAG CACCTGGGGG GGCTCTCAGG CTCCCCCGCC ACCTGGAAAT 
GGGAGGTCAC AGCCAACTTC AGGGTGGAAG GGTGGTCCCC AGCCAACTGT TTCCAAGGAG 
GAAGAGCCAA GTGGCTGGGA GGAACCATCC TCACAATCCA TTGGTCATAA AATGGACATT 
GATGACGGAA CAGCAGCTTG GGGGGATCCT AACAATTACA ATTACAAAAA TGTCAGCCTG 
TGGGACAAGA ATGCTCAAGC AGGCCCTGGG CAGATGGCTC GGGAGCAAGG AATATCTGGG 
ACATTGGCAA ACAAGGCAGC TCCCTCAGTG TGGAGTAAAA ACAAAGGACC CCCAACTGAC 
AATGGGACAT CAGCTTGGGG TGAGCCAACT GAACCTGCTT CAGGGTGGGG AGAAGGTGAG 
GAGTCGGGTC CACCAACACC AGGATGGGGA AGTGCACCCC AAGTAAATCC CAGTGCCATG 
AAATCTGGTA CAAAATCTAT GCAAGATGGC TGGGGTGAGA GTGATGGGCC GGTGACAGGA 
ACACGTCACA GCAGCTGGGA TGAAGAAGAA GAGGGTGGAG TCTGGAACAG TGCAGGTTCT 
CAGGGAAGTG GCTCCTCCCA CAACTCTGCA GGTTGGGGAC AAGGAGGAAA GAAACCACAG 
ATCAAGGGTC CCTTAAAAGG AGGGAGCAAT GATACCTGGA TGAACACCTT GCCCAAACAG 
TTCTCCAACA TGGGATTGTT GAGCCAAGTT GATGATCCCT CTACCACTAA GATGGACCAT 
CCTGGCGGGC TTCCAGATAA GAAGTTTGAG ACAGACAAGC GGGGCGTGAA TCTCGGGGAT 
TTTAATGACA TGATGCGGAA GGAACGGTCT GGCTTCCGTC TGAACAATTC CAAAGAGATG 
GGAGCTGCAG ACAGTGGGCC TTATTTTGAG AAGCTGACTT TGCCTTTCTC CAATCAAGAC 
GGGTGCCTTG GGGATGAGGC TCCCTGTTCC CCTTTTTCTC CCTCCCCTAG CTACAAGCTA 
TCTCCCTCCG GCTCCTCGCT CCCTTCCGTG GGCCTGGGGG CTATCGGTTC TGGCCTCAAC 
CCCCAAACCT TCGCTGCTAG ACAAGGTGGC AATCACGGAC TATTTGGAAA CAGCGGTGCA 
CAATCGCGAG GCATGCACAA CCCTGTGCCA TCGCTCAACT CCTCTCCCAA CCTCCGGGCA 
CAAGTGCCTC CCCAGTTCCT CTCTCCCCAG GTCTCAGCCT CCATGCTAAA GCAGTTTCCT 
AACAGTAACA TGAATCCAGG ACTTTTCAAT ATGGGGCCAC AGCTTTCCCC TCAGCAGATT 
GCAATGCTGA GCCAGCTTCC TCAAATTCAG CAGTTCCAGC TGGCATGTCA GCTCCTCTTG 
CAACAACAAC AACAGCAGCA ACAGCAGCAG CAGCAGCAGC AACTCCTTCA GAACCAGAGG 
AAGATCTCAC AAGCGGTCAG ACAGCAGCAG GAACAGCAGA TAGCACGCAT GGTAAGTGCC 
CTACAACAGC AGCAACAGCA ACAGCAGCAG CAGCAACAGA GGCAGCCAGG AATGAAGCAC 
TCTCCCTCTC ACCCTGCTGG ACCCAAAGGT CACTTAGATG CTGTTGGACT CTCAGACATG 
CAAGCCAAAG GGCACATGCC ATATGGATCT GGTTTTGGCT CAAGTGGGAT GGATTATGGT 
ACAGTAGGTA AGGAGGCGGG AGCAGAGTCT CGATTTAAAC AGTGGACATC CATGATGGAG 
GGGCTACCGT CCACTGTTTC TCAAGATACC AGCCTGCATA AAAATGGCTC CATTGCACCC 
CCAGTAAAAA ATCGTGGAGG TTCCCCCTAC CACCAATATG ACATGTTGTC TACGGATGCT 
TTGGGAGGGC ATGGGGGGCC AACAGCTGAG AGCTGGTTAC CTGCCAAATC ACCCCCTTCA 
AGCAAAATTG GAAACAAGTC AATCAATGCC AGCTGGCCCC CAGAGTTTCA GCCAGGAGTG 
CCATGGAAAG GGATCCAAAA TATTGATCCA GAATCTGATC CTTATGTTAC CCCTGGCAGT 
GTGCTTGGGG GTCCAGCCCC ATCTCCCATT GTAGATACTG ACCACCAGCT ACTGCAGGAC 
ACCCCCACAG GGTCCAATTC TTCCCTCAAC ACCTCGCTGC CTTCACCTGG TGCCTGGCCC 
TTTAGTGCCT CTGAGAATAT CCATAGCACT TCAGCACTCT CTGCAAAGTT TGGGGACTAC 
AAATCGACCT GGTCACCAGA CCCCATAGGA CATAACCCAT CACATCACTC AAACAAGATG 
TGGAAAAACC AGATGTCCAG GAACAGCCCT GGGCTGCCTC GCCCACCTCC AGGGCTCACA 
AACAACCCCA AAGCCACCAA CTCTCCCTGG AACAGCACAG CACCTCGCTC CGTCAGGGGC 
TGGGGCGGAC AGGACTCCAG AATGGCTTCT GCATCAGCTT GGAGTGATGG TGGATCTGTG 
CGCCCCAGCT ACTGGCTGAT CCTTCTTAAT CTCACACCAC AGATCGATGG TTCCACCCTG 
CGAACGATCT GCATGCAGCA TGGCCCCCTG CTGACATTCC ATCTCAACCT TACCCAGGGC 
ACAGCTCTAA TCCGATACAG CACCAAACAG GAGGCAGCCA AGGCCCAGAG TGCCCTCCAT 
ATGTGTGTTC TGGGTAATAC AACTATCCTG GCGGAGTTTG CAACGGATGA GGAGGTGAGC 
CGGTATCTTG CACAAGCTCA GCCACCAGCT CCCTCTGCTC CAGGAGCAGG CTGGCAGCCT 
TTGGAAGTGG GACCGAACCA CCCAGCTGAA CCAGTAGGGC CAGCCCTAAA CCTTTTTGGA 
GGAACCACAG GGCTAGGGCA ATGGAGCAGC GGCGGTGGTG GTAGTGACCT GTCAGGGGTT 
TCTCTCTGGG GTACTCCTAG CAGCTACACA TCCAGTCTGT GGGGTGTACC GAGCACAGAG 
GACGCCCACA GGATGGGCAG CCCTGCCCCG TTACTACCCG GTGACCTTCT GGGAGGAGGG 
ACCGACTCGA TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnrc6b ( XM_031901082.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756942.1 Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X3, mRNA.

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CloneID	OXa57434
Clone ID Related Accession (Same CDS sequence)	XM_031901082.1
Accession Version	XM_031901082.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trinucleotide repeat-containing gene 6B protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAATG GCTGCCAGTC TCTCGTTTAT GCACATTTCC CTTTAAAGAG GAGAAAACAG 
AAATATATAA ACAAGTGTGT TTGGAATTTA TTATTGTGTA TTGCATTTTT GCCTATTTTT 
ATTTTGTGTA CTTGCGTTCA TTTTTTTTGT ACTGGATTTA CCTTGTATGC CTCAAACCGC 
TGGATTATAG CACAGTTGCA CTTTATAAGA GAGAAGGAGC AAGAAAGGGA AGAACAGTTA 
ATGGAAGACA AGAAAAGGAA GAAAGAGGAT AAAAAGAAGA AAGAAGCTGC TCAGAAGGTC 
ACAGAACAAA AAACCAAAGT GCCCGAAGTG ACGAAACCAA GTTCAAGCCA AGCGACGGCC 
GCCAGCCTAC CTGGCAGCTC CCCCTCGCCA CCAGTCAATG GTGGCAACAA TGCCAAAAGG 
GTGGCAGTGC CGAACGGACA ACCGCCCAGC ACCGCCCGCT ATATGCCTCG GGAGGTGCCG 
CCGCGATTCC GCTGCCAGCA GGACCACAAA GTGTTACTGA AGCGTGGACA GCCTCCTCCA 
GCGTCCTGTA TGCTGCCGGG GGGAGGAGCA GGGACTCCCC CCTCAACCAC TGCTCCAGGG 
GCCGGCCCAG GCAACGCACA GCCAGGGGCG GGGACACTGC TGCCAGGGGA AAGCGGGGCT 
TCAACAGACT CATCATCGGG GGCAGCTGCT ACTTCAAATT ATGCAAATTC CACTTGGGGC 
TCAGGAGGCC CCACCAGCAG CGGCCCCACC ACAAACCCTC CCCACGCCTG GGACAAAGTG 
ATTGTAGACG GGTCGGACAT GGAAGAGTGG CCTTGCATAG CCGGCGTGGA CGCAGAGCCC 
CCTTCCGACA ACGCCACAGA CAACAACAGT GCCTCGAACT CAGCCTCGGA GAAGGGACCC 
CCACAAGGAA GCACCACTAG CCACAAAGGG CGAGGGGGCC AGGGCCAGCA GCAACAGCAG 
CAGCAGCCTG GTAGTGAATG TATCCAGGGC GTCTGGAAAT CTGACCCCAA GGCTAAGCCC 
GTCCCGTCTT CCAACCCTGC TTCAGAGGGT GTAAATAGAC TGGGAAACTG GAGAAATGGG 
GGTGGGTCAG AGAGAGCAGG TTCTGCCTCT GGTTTCAGCA ACTTTAATCC AAATACTAAC 
CCATCTGCTT GGCCTGCCCT TGTCCAGGAA GGCAACAGGA AAGGGAACCC AGAGTCAGAC 
AGCAGCTTTT CTGGCGCACA ACTCGGCCCA GTGGGTTTGA CCTCTCGGGA GCAGCAGTCA 
AAGATGGAAA ATGCAGGTTC CAGCTTTGCA GGATCGGGCA GGGAGTCTGC CACAACCCAT 
CACACTGACG GACCAAAAAA TGGAAACACT AACTCTACGA ACTCAAATAC TTTGAACCCT 
CCTGATAACA AGGGAACAGC TTTTGGGGTG GGCTCAATGG ATTCCTGCCG GGGTTCAGAC 
TCCCCTGCTC AAAGCACTGG AGAAAGAAAG AATGGGGGGA TTGGCTCTTG GGGAGCGGGA 
AGGGGTCAGT CTGCCACAGG AGATGCTAAT TGTGGACATA GCAACTCTGG GAGCAATGGA 
CGAGAGAGAG AAGACGCAAG GAAAGCGCCT TCATCTCATA GACCCAATGG GTCAAGGGGG 
GAATCTTGGG AAAGCAAAAG TGGGAATGGT TCTTGGGGTT ACAGCCCGCA GGCAGACCTC 
AGTGAAGTGA AATGGAGTGA AGGGAACAAA TTGGGGGCTG GAGGGGCGTC TCAGGGAGAA 
TGGAAGCAGC CAGCCGGGCA TGAGGACTTA AAGGTGGGAG AGTGGGGAGG CTTGAACCAA 
GGAAACTCTA GCACTGGAGC ATGGGACAAT CAGAAGGGCC ACTCTCTATC GGATAACCAG 
GGCAACACGT CATCAGCAGC CTGCTGGGGC CGAGCCCCCA GCTCTGCAGG AAGTGAAGCT 
GGGGGGCAAA GCACTGGAAG CAACGCTAAA GCAGGGGGGA GCGGAGGAAG CGGAGACAGC 
CTGAACTCCA GCAGTCGACG TTCACACAGA CCTGCCCTCC CAGACAGTCA GGCTGCTCTT 
CAGACTTTAC TGAATCGCAC TGATTTGGAT CCCCGGGTGC TGTCCAACTC TGGCTGGGGC 
CAAACCCAGA TCAAGCAAGA TGCATCATGG GAGAGTGAGG AAGTGCCCTC TCGAACTGAA 
GGCAAATCAG ACCGTGGGAC TGAAGGTTGG GAGAACGTGT CCCAAGCCAA AACCTCAGGG 
GGTTGGGGGG ATGCTCCCAG CCAAAGCAAT CAAAACAAGT CAGGCTGGGG GGAGCCCCCT 
GTCCCTGCAC CTTCAACCCA AGAATGGAAG GACACCAAAG CAACTGGCTG GAATGACTAT 
AAAAACAGCC CAACTGGTTG GGTTGGTGGA GGAGGTGGTG GGGTGGTTGG AGCACGACCA 
GAGGACAAGG CAGTTCCAGG TTGGAACAAT GAAAGTGGTG GGGCTGCGGA GCCTGGGTGG 
AGTACTCGCC AGTCTAATCC AGGAGGTGGC TGGTCAGCAG GAAAGAATGG TTGGGGGGGA 
AATGGTAGTG AGGACATGGA GCCTCCCAAG GCCCCTGGAG GCTGGGAAGG CTCAGGAAGC 
AAATCCTCCT CTGGCTGGGG TGAAGTTGGA CAAAATGATG TGGGTTCTTG GGGTAATGGG 
GCAAACTCAA ATCCAGCTCC ACGAACTGGC TGGGAGGAGA CTAAGAGGCC CCAGGGAGGA 
GGAAACTGGG GTGAGGCAGC TAGGCCCCAA CTACCCTGGA ACAAACAGCA GGAATCAACG 
CCATCTTCAG CAACTCCTAG CACCTGGGGG GGCTCTCAGG CTCCCCCGCC ACCTGGAAAT 
GGGAGGTCAC AGCCAACTTC AGGGTGGAAG GGTGGTCCCC AGCCAACTGT TTCCAAGGAG 
GAAGAGCCAA GTGGCTGGGA GGAACCATCC TCACAATCCA TTGGTCATAA AATGGACATT 
GATGACGGAA CAGCAGCTTG GGGGGATCCT AACAATTACA ATTACAAAAA TGTCAGCCTG 
TGGGACAAGA ATGCTCAAGC AGGCCCTGGG CAGATGGCTC GGGAGCAAGG AATATCTGGG 
ACATTGGCAA ACAAGGCAGC TCCCTCAGTG TGGAGTAAAA ACAAAGGACC CCCAACTGAC 
AATGGGACAT CAGCTTGGGG TGAGCCAACT GAACCTGCTT CAGGGTGGGG AGAAGGTGAG 
GAGTCGGGTC CACCAACACC AGGATGGGGA AGTGCACCCC AAGTAAATCC CAGTGCCATG 
AAATCTGGTA CAAAATCTAT GCAAGATGGC TGGGGTGAGA GTGATGGGCC GGTGACAGGA 
ACACGTCACA GCAGCTGGGA TGAAGAAGAA GAGGGTGGAG TCTGGAACAG TGCAGGTTCT 
CAGGGAAGTG GCTCCTCCCA CAACTCTGCA GGTTGGGGAC AAGGAGGAAA GAAACCACAG 
ATCAAGGGTC CCTTAAAAGG AGGGAGCAAT GATACCTGGA TGAACACCTT GCCCAAACAG 
TTCTCCAACA TGGGATTGTT GAGCCAAGTT GATGATCCCT CTACCACTAA GATGGACCAT 
CCTGGCGGGC TTCCAGATAA GAAGTTTGAG ACAGACAAGC GGGGCGTGAA TCTCGGGGAT 
TTTAATGACA TGATGCGGAA GGAACGGTCT GGCTTCCGTC TGAACAATTC CAAAGAGATG 
GGAGCTGCAG ACAGTGGGCC TTATTTTGAG AAGGGTGGCA ATCACGGACT ATTTGGAAAC 
AGCGGTGCAC AATCGCGAGG CATGCACAAC CCTGTGCCAT CGCTCAACTC CTCTCCCAAC 
CTCCGGGCAC AAGTGCCTCC CCAGTTCCTC TCTCCCCAGG TCTCAGCCTC CATGCTAAAG 
CAGTTTCCTA ACAGTAACAT GAATCCAGGA CTTTTCAATA TGGGGCCACA GCTTTCCCCT 
CAGCAGATTG CAATGCTGAG CCAGCTTCCT CAAATTCAGC AGTTCCAGCT GGCATGTCAG 
CTCCTCTTGC AACAACAACA ACAGCAGCAA CAGCAGCAGC AGCAGCAGCA ACTCCTTCAG 
AACCAGAGGA AGATCTCACA AGCGGTCAGA CAGCAGCAGG AACAGCAGCA GATAGCACGC 
ATGGTAAGTG CCCTACAACA GCAGCAACAG CAACAGCAGC AGCAGCAACA GAGGCAGCCA 
GGAATGAAGC ACTCTCCCTC TCACCCTGCT GGACCCAAAG GTCACTTAGA TGCTGTTGGA 
CTCTCAGACA TGCAAGCCAA AGGGCACATG CCATATGGAT CTGGTTTTGG CTCAAGTGGG 
ATGGATTATG GTACAGTAGG TAAGGAGGCG GGAGCAGAGT CTCGATTTAA ACAGTGGACA 
TCCATGATGG AGGGGCTACC GTCCACTGTT TCTCAAGATA CCAGCCTGCA TAAAAATGGC 
TCCATTGCAC CCCCAGTAAA AAATCGTGGA GGTTCCCCCT ACCACCAATA TGACATGTTG 
TCTACGGATG CTTTGGGAGG GCATGGGGGG CCAACAGCTG AGAGCTGGTT ACCTGCCAAA 
TCACCCCCTT CAAGCAAAAT TGGAAACAAG TCAATCAATG CCAGCTGGCC CCCAGAGTTT 
CAGCCAGGAG TGCCATGGAA AGGGATCCAA AATATTGATC CAGAATCTGA TCCTTATGTT 
ACCCCTGGCA GTGTGCTTGG GGGTCCAGCC CCATCTCCCA TTGTAGATAC TGACCACCAG 
CTACTGCAGG ACACCCCCAC AGGGTCCAAT TCTTCCCTCA ACACCTCGCT GCCTTCACCT 
GGTGCCTGGC CCTTTAGTGC CTCTGAGAAT ATCCATAGCA CTTCAGCACT CTCTGCAAAG 
TTTGGGGACT ACAAATCGAC CTGGTCACCA GACCCCATAG GACATAACCC ATCACATCAC 
TCAAACAAGA TGTGGAAAAA CCAGATGTCC AGGAACAGCC CTGGGCTGCC TCGCCCACCT 
CCAGGGCTCA CAAACAACCC CAAAGCCACC AACTCTCCCT GGAACAGCAC AGCACCTCGC 
TCCGTCAGGG GCTGGGGCGG ACAGGACTCC AGAATGGCTT CTGCATCAGC TTGGAGTGAT 
GGTGGATCTG TGCGCCCCAG CTACTGGCTG ATCCTTCTTA ATCTCACACC ACAGATCGAT 
GGTTCCACCC TGCGAACGAT CTGCATGCAG CATGGCCCCC TGCTGACATT CCATCTCAAC 
CTTACCCAGG GCACAGCTCT AATCCGATAC AGCACCAAAC AGGAGGCAGC CAAGGCCCAG 
AGTGCCCTCC ATATGTGTGT TCTGGGTAAT ACAACTATCC TGGCGGAGTT TGCAACGGAT 
GAGGAGGTGA GCCGGTATCT TGCACAAGCT CAGCCACCAG CTCCCTCTGC TCCAGGAGCA 
GGCTGGCAGC CTTTGGAAGT GGGACCGAAC CACCCAGCTG AACCAGTAGG GCCAGCCCTA 
AACCTTTTTG GAGGAACCAC AGGGCTAGGG CAATGGAGCA GCGGCGGTGG TGGTAGTGAC 
CTGTCAGGGG TTTCTCTCTG GGGTACTCCT AGCAGCTACA CATCCAGTCT GTGGGGTGTA 
CCGAGCACAG AGGACGCCCA CAGGATGGGC AGCCCTGCCC CGTTACTACC CGGTGACCTT 
CTGGGAGGAG GGACCGACTC GATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756942.1
CDS	53..5659
Translation	MQNGCQSLVYAHFPLKRRKQKYINKCVWNLLLCIAFLPIFILCTCVHFFCTGFTLYASNRWIIAQLHFIREKEQEREEQLMEDKKRKKEDKKKKEAAQKVTEQKTKVPEVTKPSSSQATAASLPGSSPSPPVNGGNNAKRVAVPNGQPPSTARYMPREVPPRFRCQQDHKVLLKRGQPPPASCMLPGGGAGTPPSTTAPGAGPGNAQPGAGTLLPGESGASTDSSSGAAATSNYANSTWGSGGPTSSGPTTNPPHAWDKVIVDGSDMEEWPCIAGVDAEPPSDNATDNNSASNSASEKGPPQGSTTSHKGRGGQGQQQQQQQPGSECIQGVWKSDPKAKPVPSSNPASEGVNRLGNWRNGGGSERAGSASGFSNFNPNTNPSAWPALVQEGNRKGNPESDSSFSGAQLGPVGLTSREQQSKMENAGSSFAGSGRESATTHHTDGPKNGNTNSTNSNTLNPPDNKGTAFGVGSMDSCRGSDSPAQSTGERKNGGIGSWGAGRGQSATGDANCGHSNSGSNGREREDARKAPSSHRPNGSRGESWESKSGNGSWGYSPQADLSEVKWSEGNKLGAGGASQGEWKQPAGHEDLKVGEWGGLNQGNSSTGAWDNQKGHSLSDNQGNTSSAACWGRAPSSAGSEAGGQSTGSNAKAGGSGGSGDSLNSSSRRSHRPALPDSQAALQTLLNRTDLDPRVLSNSGWGQTQIKQDASWESEEVPSRTEGKSDRGTEGWENVSQAKTSGGWGDAPSQSNQNKSGWGEPPVPAPSTQEWKDTKATGWNDYKNSPTGWVGGGGGGVVGARPEDKAVPGWNNESGGAAEPGWSTRQSNPGGGWSAGKNGWGGNGSEDMEPPKAPGGWEGSGSKSSSGWGEVGQNDVGSWGNGANSNPAPRTGWEETKRPQGGGNWGEAARPQLPWNKQQESTPSSATPSTWGGSQAPPPPGNGRSQPTSGWKGGPQPTVSKEEEPSGWEEPSSQSIGHKMDIDDGTAAWGDPNNYNYKNVSLWDKNAQAGPGQMAREQGISGTLANKAAPSVWSKNKGPPTDNGTSAWGEPTEPASGWGEGEESGPPTPGWGSAPQVNPSAMKSGTKSMQDGWGESDGPVTGTRHSSWDEEEEGGVWNSAGSQGSGSSHNSAGWGQGGKKPQIKGPLKGGSNDTWMNTLPKQFSNMGLLSQVDDPSTTKMDHPGGLPDKKFETDKRGVNLGDFNDMMRKERSGFRLNNSKEMGAADSGPYFEKGGNHGLFGNSGAQSRGMHNPVPSLNSSPNLRAQVPPQFLSPQVSASMLKQFPNSNMNPGLFNMGPQLSPQQIAMLSQLPQIQQFQLACQLLLQQQQQQQQQQQQQQLLQNQRKISQAVRQQQEQQQIARMVSALQQQQQQQQQQQQRQPGMKHSPSHPAGPKGHLDAVGLSDMQAKGHMPYGSGFGSSGMDYGTVGKEAGAESRFKQWTSMMEGLPSTVSQDTSLHKNGSIAPPVKNRGGSPYHQYDMLSTDALGGHGGPTAESWLPAKSPPSSKIGNKSINASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGPAPSPIVDTDHQLLQDTPTGSNSSLNTSLPSPGAWPFSASENIHSTSALSAKFGDYKSTWSPDPIGHNPSHHSNKMWKNQMSRNSPGLPRPPPGLTNNPKATNSPWNSTAPRSVRGWGGQDSRMASASAWSDGGSVRPSYWLILLNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQSALHMCVLGNTTILAEFATDEEVSRYLAQAQPPAPSAPGAGWQPLEVGPNHPAEPVGPALNLFGGTTGLGQWSSGGGGSDLSGVSLWGTPSSYTSSLWGVPSTEDAHRMGSPAPLLPGDLLGGGTDSI

Target ORF information:

RefSeq Version	XM_031901082.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901082.1

ORF Insert Sequence:

ATGCAAAATG GCTGCCAGTC TCTCGTTTAT GCACATTTCC CTTTAAAGAG GAGAAAACAG 
AAATATATAA ACAAGTGTGT TTGGAATTTA TTATTGTGTA TTGCATTTTT GCCTATTTTT 
ATTTTGTGTA CTTGCGTTCA TTTTTTTTGT ACTGGATTTA CCTTGTATGC CTCAAACCGC 
TGGATTATAG CACAGTTGCA CTTTATAAGA GAGAAGGAGC AAGAAAGGGA AGAACAGTTA 
ATGGAAGACA AGAAAAGGAA GAAAGAGGAT AAAAAGAAGA AAGAAGCTGC TCAGAAGGTC 
ACAGAACAAA AAACCAAAGT GCCCGAAGTG ACGAAACCAA GTTCAAGCCA AGCGACGGCC 
GCCAGCCTAC CTGGCAGCTC CCCCTCGCCA CCAGTCAATG GTGGCAACAA TGCCAAAAGG 
GTGGCAGTGC CGAACGGACA ACCGCCCAGC ACCGCCCGCT ATATGCCTCG GGAGGTGCCG 
CCGCGATTCC GCTGCCAGCA GGACCACAAA GTGTTACTGA AGCGTGGACA GCCTCCTCCA 
GCGTCCTGTA TGCTGCCGGG GGGAGGAGCA GGGACTCCCC CCTCAACCAC TGCTCCAGGG 
GCCGGCCCAG GCAACGCACA GCCAGGGGCG GGGACACTGC TGCCAGGGGA AAGCGGGGCT 
TCAACAGACT CATCATCGGG GGCAGCTGCT ACTTCAAATT ATGCAAATTC CACTTGGGGC 
TCAGGAGGCC CCACCAGCAG CGGCCCCACC ACAAACCCTC CCCACGCCTG GGACAAAGTG 
ATTGTAGACG GGTCGGACAT GGAAGAGTGG CCTTGCATAG CCGGCGTGGA CGCAGAGCCC 
CCTTCCGACA ACGCCACAGA CAACAACAGT GCCTCGAACT CAGCCTCGGA GAAGGGACCC 
CCACAAGGAA GCACCACTAG CCACAAAGGG CGAGGGGGCC AGGGCCAGCA GCAACAGCAG 
CAGCAGCCTG GTAGTGAATG TATCCAGGGC GTCTGGAAAT CTGACCCCAA GGCTAAGCCC 
GTCCCGTCTT CCAACCCTGC TTCAGAGGGT GTAAATAGAC TGGGAAACTG GAGAAATGGG 
GGTGGGTCAG AGAGAGCAGG TTCTGCCTCT GGTTTCAGCA ACTTTAATCC AAATACTAAC 
CCATCTGCTT GGCCTGCCCT TGTCCAGGAA GGCAACAGGA AAGGGAACCC AGAGTCAGAC 
AGCAGCTTTT CTGGCGCACA ACTCGGCCCA GTGGGTTTGA CCTCTCGGGA GCAGCAGTCA 
AAGATGGAAA ATGCAGGTTC CAGCTTTGCA GGATCGGGCA GGGAGTCTGC CACAACCCAT 
CACACTGACG GACCAAAAAA TGGAAACACT AACTCTACGA ACTCAAATAC TTTGAACCCT 
CCTGATAACA AGGGAACAGC TTTTGGGGTG GGCTCAATGG ATTCCTGCCG GGGTTCAGAC 
TCCCCTGCTC AAAGCACTGG AGAAAGAAAG AATGGGGGGA TTGGCTCTTG GGGAGCGGGA 
AGGGGTCAGT CTGCCACAGG AGATGCTAAT TGTGGACATA GCAACTCTGG GAGCAATGGA 
CGAGAGAGAG AAGACGCAAG GAAAGCGCCT TCATCTCATA GACCCAATGG GTCAAGGGGG 
GAATCTTGGG AAAGCAAAAG TGGGAATGGT TCTTGGGGTT ACAGCCCGCA GGCAGACCTC 
AGTGAAGTGA AATGGAGTGA AGGGAACAAA TTGGGGGCTG GAGGGGCGTC TCAGGGAGAA 
TGGAAGCAGC CAGCCGGGCA TGAGGACTTA AAGGTGGGAG AGTGGGGAGG CTTGAACCAA 
GGAAACTCTA GCACTGGAGC ATGGGACAAT CAGAAGGGCC ACTCTCTATC GGATAACCAG 
GGCAACACGT CATCAGCAGC CTGCTGGGGC CGAGCCCCCA GCTCTGCAGG AAGTGAAGCT 
GGGGGGCAAA GCACTGGAAG CAACGCTAAA GCAGGGGGGA GCGGAGGAAG CGGAGACAGC 
CTGAACTCCA GCAGTCGACG TTCACACAGA CCTGCCCTCC CAGACAGTCA GGCTGCTCTT 
CAGACTTTAC TGAATCGCAC TGATTTGGAT CCCCGGGTGC TGTCCAACTC TGGCTGGGGC 
CAAACCCAGA TCAAGCAAGA TGCATCATGG GAGAGTGAGG AAGTGCCCTC TCGAACTGAA 
GGCAAATCAG ACCGTGGGAC TGAAGGTTGG GAGAACGTGT CCCAAGCCAA AACCTCAGGG 
GGTTGGGGGG ATGCTCCCAG CCAAAGCAAT CAAAACAAGT CAGGCTGGGG GGAGCCCCCT 
GTCCCTGCAC CTTCAACCCA AGAATGGAAG GACACCAAAG CAACTGGCTG GAATGACTAT 
AAAAACAGCC CAACTGGTTG GGTTGGTGGA GGAGGTGGTG GGGTGGTTGG AGCACGACCA 
GAGGACAAGG CAGTTCCAGG TTGGAACAAT GAAAGTGGTG GGGCTGCGGA GCCTGGGTGG 
AGTACTCGCC AGTCTAATCC AGGAGGTGGC TGGTCAGCAG GAAAGAATGG TTGGGGGGGA 
AATGGTAGTG AGGACATGGA GCCTCCCAAG GCCCCTGGAG GCTGGGAAGG CTCAGGAAGC 
AAATCCTCCT CTGGCTGGGG TGAAGTTGGA CAAAATGATG TGGGTTCTTG GGGTAATGGG 
GCAAACTCAA ATCCAGCTCC ACGAACTGGC TGGGAGGAGA CTAAGAGGCC CCAGGGAGGA 
GGAAACTGGG GTGAGGCAGC TAGGCCCCAA CTACCCTGGA ACAAACAGCA GGAATCAACG 
CCATCTTCAG CAACTCCTAG CACCTGGGGG GGCTCTCAGG CTCCCCCGCC ACCTGGAAAT 
GGGAGGTCAC AGCCAACTTC AGGGTGGAAG GGTGGTCCCC AGCCAACTGT TTCCAAGGAG 
GAAGAGCCAA GTGGCTGGGA GGAACCATCC TCACAATCCA TTGGTCATAA AATGGACATT 
GATGACGGAA CAGCAGCTTG GGGGGATCCT AACAATTACA ATTACAAAAA TGTCAGCCTG 
TGGGACAAGA ATGCTCAAGC AGGCCCTGGG CAGATGGCTC GGGAGCAAGG AATATCTGGG 
ACATTGGCAA ACAAGGCAGC TCCCTCAGTG TGGAGTAAAA ACAAAGGACC CCCAACTGAC 
AATGGGACAT CAGCTTGGGG TGAGCCAACT GAACCTGCTT CAGGGTGGGG AGAAGGTGAG 
GAGTCGGGTC CACCAACACC AGGATGGGGA AGTGCACCCC AAGTAAATCC CAGTGCCATG 
AAATCTGGTA CAAAATCTAT GCAAGATGGC TGGGGTGAGA GTGATGGGCC GGTGACAGGA 
ACACGTCACA GCAGCTGGGA TGAAGAAGAA GAGGGTGGAG TCTGGAACAG TGCAGGTTCT 
CAGGGAAGTG GCTCCTCCCA CAACTCTGCA GGTTGGGGAC AAGGAGGAAA GAAACCACAG 
ATCAAGGGTC CCTTAAAAGG AGGGAGCAAT GATACCTGGA TGAACACCTT GCCCAAACAG 
TTCTCCAACA TGGGATTGTT GAGCCAAGTT GATGATCCCT CTACCACTAA GATGGACCAT 
CCTGGCGGGC TTCCAGATAA GAAGTTTGAG ACAGACAAGC GGGGCGTGAA TCTCGGGGAT 
TTTAATGACA TGATGCGGAA GGAACGGTCT GGCTTCCGTC TGAACAATTC CAAAGAGATG 
GGAGCTGCAG ACAGTGGGCC TTATTTTGAG AAGGGTGGCA ATCACGGACT ATTTGGAAAC 
AGCGGTGCAC AATCGCGAGG CATGCACAAC CCTGTGCCAT CGCTCAACTC CTCTCCCAAC 
CTCCGGGCAC AAGTGCCTCC CCAGTTCCTC TCTCCCCAGG TCTCAGCCTC CATGCTAAAG 
CAGTTTCCTA ACAGTAACAT GAATCCAGGA CTTTTCAATA TGGGGCCACA GCTTTCCCCT 
CAGCAGATTG CAATGCTGAG CCAGCTTCCT CAAATTCAGC AGTTCCAGCT GGCATGTCAG 
CTCCTCTTGC AACAACAACA ACAGCAGCAA CAGCAGCAGC AGCAGCAGCA ACTCCTTCAG 
AACCAGAGGA AGATCTCACA AGCGGTCAGA CAGCAGCAGG AACAGCAGCA GATAGCACGC 
ATGGTAAGTG CCCTACAACA GCAGCAACAG CAACAGCAGC AGCAGCAACA GAGGCAGCCA 
GGAATGAAGC ACTCTCCCTC TCACCCTGCT GGACCCAAAG GTCACTTAGA TGCTGTTGGA 
CTCTCAGACA TGCAAGCCAA AGGGCACATG CCATATGGAT CTGGTTTTGG CTCAAGTGGG 
ATGGATTATG GTACAGTAGG TAAGGAGGCG GGAGCAGAGT CTCGATTTAA ACAGTGGACA 
TCCATGATGG AGGGGCTACC GTCCACTGTT TCTCAAGATA CCAGCCTGCA TAAAAATGGC 
TCCATTGCAC CCCCAGTAAA AAATCGTGGA GGTTCCCCCT ACCACCAATA TGACATGTTG 
TCTACGGATG CTTTGGGAGG GCATGGGGGG CCAACAGCTG AGAGCTGGTT ACCTGCCAAA 
TCACCCCCTT CAAGCAAAAT TGGAAACAAG TCAATCAATG CCAGCTGGCC CCCAGAGTTT 
CAGCCAGGAG TGCCATGGAA AGGGATCCAA AATATTGATC CAGAATCTGA TCCTTATGTT 
ACCCCTGGCA GTGTGCTTGG GGGTCCAGCC CCATCTCCCA TTGTAGATAC TGACCACCAG 
CTACTGCAGG ACACCCCCAC AGGGTCCAAT TCTTCCCTCA ACACCTCGCT GCCTTCACCT 
GGTGCCTGGC CCTTTAGTGC CTCTGAGAAT ATCCATAGCA CTTCAGCACT CTCTGCAAAG 
TTTGGGGACT ACAAATCGAC CTGGTCACCA GACCCCATAG GACATAACCC ATCACATCAC 
TCAAACAAGA TGTGGAAAAA CCAGATGTCC AGGAACAGCC CTGGGCTGCC TCGCCCACCT 
CCAGGGCTCA CAAACAACCC CAAAGCCACC AACTCTCCCT GGAACAGCAC AGCACCTCGC 
TCCGTCAGGG GCTGGGGCGG ACAGGACTCC AGAATGGCTT CTGCATCAGC TTGGAGTGAT 
GGTGGATCTG TGCGCCCCAG CTACTGGCTG ATCCTTCTTA ATCTCACACC ACAGATCGAT 
GGTTCCACCC TGCGAACGAT CTGCATGCAG CATGGCCCCC TGCTGACATT CCATCTCAAC 
CTTACCCAGG GCACAGCTCT AATCCGATAC AGCACCAAAC AGGAGGCAGC CAAGGCCCAG 
AGTGCCCTCC ATATGTGTGT TCTGGGTAAT ACAACTATCC TGGCGGAGTT TGCAACGGAT 
GAGGAGGTGA GCCGGTATCT TGCACAAGCT CAGCCACCAG CTCCCTCTGC TCCAGGAGCA 
GGCTGGCAGC CTTTGGAAGT GGGACCGAAC CACCCAGCTG AACCAGTAGG GCCAGCCCTA 
AACCTTTTTG GAGGAACCAC AGGGCTAGGG CAATGGAGCA GCGGCGGTGG TGGTAGTGAC 
CTGTCAGGGG TTTCTCTCTG GGGTACTCCT AGCAGCTACA CATCCAGTCT GTGGGGTGTA 
CCGAGCACAG AGGACGCCCA CAGGATGGGC AGCCCTGCCC CGTTACTACC CGGTGACCTT 
CTGGGAGGAG GGACCGACTC GATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnrc6b ( XM_012961477.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816931.2 Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X4, mRNA.

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CloneID	OXa57435
Clone ID Related Accession (Same CDS sequence)	XM_012961477.3 , XM_002934209.5 , XM_012961478.3
Accession Version	XM_012961477.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trinucleotide repeat-containing gene 6B protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961477.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAGA AACACTTGAA GAAAGCTGTT CCCAGAGAGA AGGAGCAAGA AAGGGAAGAA 
CAGTTAATGG AAGACAAGAA AAGGAAGAAA GAGGATAAAA AGAAGAAAGA AGCTGCTCAG 
AAGGTCACAG AACAAAAAAC CAAAGTGCCC GAAGTGACGA AACCAAGTTC AAGCCAAGCG 
ACGGCCGCCA GCCTACCTGG CAGCTCCCCC TCGCCACCAG TCAATGGTGG CAACAATGCC 
AAAAGGGTGG CAGTGCCGAA CGGACAACCG CCCAGCACCG CCCGCTATAT GCCTCGGGAG 
GTGCCGCCGC GATTCCGCTG CCAGCAGGAC CACAAAGTGT TACTGAAGCG TGGACAGCCT 
CCTCCAGCGT CCTGTATGCT GCCGGGGGGA GGAGCAGGGA CTCCCCCCTC AACCACTGCT 
CCAGGGGCCG GCCCAGGCAA CGCACAGCCA GGGGCGGGGA CACTGCTGCC AGGGGAAAGC 
GGGGCTTCAA CAGACTCATC ATCGGGGGCA GCTGCTACTT CAAATTATGC AAATTCCACT 
TGGGGCTCAG GAGGCCCCAC CAGCAGCGGC CCCACCACAA ACCCTCCCCA CGCCTGGGAC 
AAAGTGATTG TAGACGGGTC GGACATGGAA GAGTGGCCTT GCATAGCCGG CGTGGACGCA 
GAGCCCCCTT CCGACAACGC CACAGACAAC AACAGTGCCT CGAACTCAGC CTCGGAGAAG 
GGACCCCCAC AAGGAAGCAC CACTAGCCAC AAAGGGCGAG GGGGCCAGGG CCAGCAGCAA 
CAGCAGCAGC AGCCTGGTAG TGAATGTATC CAGGGCGTCT GGAAATCTGA CCCCAAGGCT 
AAGCCCGTCC CGTCTTCCAA CCCTGCTTCA GAGGGTGTAA ATAGACTGGG AAACTGGAGA 
AATGGGGGTG GGTCAGAGAG AGCAGGTTCT GCCTCTGGTT TCAGCAACTT TAATCCAAAT 
ACTAACCCAT CTGCTTGGCC TGCCCTTGTC CAGGAAGGCA ACAGGAAAGG GAACCCAGAG 
TCAGACAGCA GCTTTTCTGG CGCACAACTC GGCCCAGTGG GTTTGACCTC TCGGGAGCAG 
CAGTCAAAGA TGGAAAATGC AGGTTCCAGC TTTGCAGGAT CGGGCAGGGA GTCTGCCACA 
ACCCATCACA CTGACGGACC AAAAAATGGA AACACTAACT CTACGAACTC AAATACTTTG 
AACCCTCCTG ATAACAAGGG AACAGCTTTT GGGGTGGGCT CAATGGATTC CTGCCGGGGT 
TCAGACTCCC CTGCTCAAAG CACTGGAGAA AGAAAGAATG GGGGGATTGG CTCTTGGGGA 
GCGGGAAGGG GTCAGTCTGC CACAGGAGAT GCTAATTGTG GACATAGCAA CTCTGGGAGC 
AATGGACGAG AGAGAGAAGA CGCAAGGAAA GCGCCTTCAT CTCATAGACC CAATGGGTCA 
AGGGGGGAAT CTTGGGAAAG CAAAAGTGGG AATGGTTCTT GGGGTTACAG CCCGCAGGCA 
GACCTCAGTG AAGTGAAATG GAGTGAAGGG AACAAATTGG GGGCTGGAGG GGCGTCTCAG 
GGAGAATGGA AGCAGCCAGC CGGGCATGAG GACTTAAAGG TGGGAGAGTG GGGAGGCTTG 
AACCAAGGAA ACTCTAGCAC TGGAGCATGG GACAATCAGA AGGGCCACTC TCTATCGGAT 
AACCAGGGCA ACACGTCATC AGCAGCCTGC TGGGGCCGAG CCCCCAGCTC TGCAGGAAGT 
GAAGCTGGGG GGCAAAGCAC TGGAAGCAAC GCTAAAGCAG GGGGGAGCGG AGGAAGCGGA 
GACAGCCTGA ACTCCAGCAG TCGACGTTCA CACAGACCTG CCCTCCCAGA CAGTCAGGCT 
GCTCTTCAGA CTTTACTGAA TCGCACTGAT TTGGATCCCC GGGTGCTGTC CAACTCTGGC 
TGGGGCCAAA CCCAGATCAA GCAAGATGCA TCATGGGAGA GTGAGGAAGT GCCCTCTCGA 
ACTGAAGGCA AATCAGACCG TGGGACTGAA GGTTGGGAGA ACGTGTCCCA AGCCAAAACC 
TCAGGGGGTT GGGGGGATGC TCCCAGCCAA AGCAATCAAA ACAAGTCAGG CTGGGGGGAG 
CCCCCTGTCC CTGCACCTTC AACCCAAGAA TGGAAGGACA CCAAAGCAAC TGGCTGGAAT 
GACTATAAAA ACAGCCCAAC TGGTTGGGTT GGTGGAGGAG GTGGTGGGGT GGTTGGAGCA 
CGACCAGAGG ACAAGGCAGT TCCAGGTTGG AACAATGAAA GTGGTGGGGC TGCGGAGCCT 
GGGTGGAGTA CTCGCCAGTC TAATCCAGGA GGTGGCTGGT CAGCAGGAAA GAATGGTTGG 
GGGGGAAATG GTAGTGAGGA CATGGAGCCT CCCAAGGCCC CTGGAGGCTG GGAAGGCTCA 
GGAAGCAAAT CCTCCTCTGG CTGGGGTGAA GTTGGACAAA ATGATGTGGG TTCTTGGGGT 
AATGGGGCAA ACTCAAATCC AGCTCCACGA ACTGGCTGGG AGGAGACTAA GAGGCCCCAG 
GGAGGAGGAA ACTGGGGTGA GGCAGCTAGG CCCCAACTAC CCTGGAACAA ACAGCAGGAA 
TCAACGCCAT CTTCAGCAAC TCCTAGCACC TGGGGGGGCT CTCAGGCTCC CCCGCCACCT 
GGAAATGGGA GGTCACAGCC AACTTCAGGG TGGAAGGGTG GTCCCCAGCC AACTGTTTCC 
AAGGAGGAAG AGCCAAGTGG CTGGGAGGAA CCATCCTCAC AATCCATTGG TCATAAAATG 
GACATTGATG ACGGAACAGC AGCTTGGGGG GATCCTAACA ATTACAATTA CAAAAATGTC 
AGCCTGTGGG ACAAGAATGC TCAAGCAGGC CCTGGGCAGA TGGCTCGGGA GCAAGGAATA 
TCTGGGACAT TGGCAAACAA GGCAGCTCCC TCAGTGTGGA GTAAAAACAA AGGACCCCCA 
ACTGACAATG GGACATCAGC TTGGGGTGAG CCAACTGAAC CTGCTTCAGG GTGGGGAGAA 
GGTGAGGAGT CGGGTCCACC AACACCAGGA TGGGGAAGTG CACCCCAAGT AAATCCCAGT 
GCCATGAAAT CTGGTACAAA ATCTATGCAA GATGGCTGGG GTGAGAGTGA TGGGCCGGTG 
ACAGGAACAC GTCACAGCAG CTGGGATGAA GAAGAAGAGG GTGGAGTCTG GAACAGTGCA 
GGTTCTCAGG GAAGTGGCTC CTCCCACAAC TCTGCAGGTT GGGGACAAGG AGGAAAGAAA 
CCACAGATCA AGGGTCCCTT AAAAGGAGGG AGCAATGATA CCTGGATGAA CACCTTGCCC 
AAACAGTTCT CCAACATGGG ATTGTTGAGC CAAGTTGATG ATCCCTCTAC CACTAAGATG 
GACCATCCTG GCGGGCTTCC AGATAAGAAG TTTGAGACAG ACAAGCGGGG CGTGAATCTC 
GGGGATTTTA ATGACATGAT GCGGAAGGAA CGGTCTGGCT TCCGTCTGAA CAATTCCAAA 
GAGATGGGAG CTGCAGACAG TGGGCCTTAT TTTGAGAAGC TGACTTTGCC TTTCTCCAAT 
CAAGACGGGT GCCTTGGGGA TGAGGCTCCC TGTTCCCCTT TTTCTCCCTC CCCTAGCTAC 
AAGCTATCTC CCTCCGGCTC CTCGCTCCCT TCCGTGGGCC TGGGGGCTAT CGGTTCTGGC 
CTCAACCCCC AAACCTTCGC TGCTAGACAA GGTGGCAATC ACGGACTATT TGGAAACAGC 
GGTGCACAAT CGCGAGGCAT GCACAACCCT GTGCCATCGC TCAACTCCTC TCCCAACCTC 
CGGGCACAAG TGCCTCCCCA GTTCCTCTCT CCCCAGGTCT CAGCCTCCAT GCTAAAGCAG 
TTTCCTAACA GTAACATGAA TCCAGGACTT TTCAATATGG GGCCACAGCT TTCCCCTCAG 
CAGATTGCAA TGCTGAGCCA GCTTCCTCAA ATTCAGCAGT TCCAGCTGGC ATGTCAGCTC 
CTCTTGCAAC AACAACAACA GCAGCAACAG CAGCAGCAGC AGCAGCAACT CCTTCAGAAC 
CAGAGGAAGA TCTCACAAGC GGTCAGACAG CAGCAGGAAC AGCAGCAGAT AGCACGCATG 
GTAAGTGCCC TACAACAGCA GCAACAGCAA CAGCAGCAGC AGCAACAGAG GCAGCCAGGA 
ATGAAGCACT CTCCCTCTCA CCCTGCTGGA CCCAAAGGTC ACTTAGATGC TGTTGGACTC 
TCAGACATGC AAGCCAAAGG GCACATGCCA TATGGATCTG GTTTTGGCTC AAGTGGGATG 
GATTATGGTA CAGTAGGTAA GGAGGCGGGA GCAGAGTCTC GATTTAAACA GTGGACATCC 
ATGATGGAGG GGCTACCGTC CACTGTTTCT CAAGATACCA GCCTGCATAA AAATGGCTCC 
ATTGCACCCC CAGTAAAAAA TCGTGGAGGT TCCCCCTACC ACCAATATGA CATGTTGTCT 
ACGGATGCTT TGGGAGGGCA TGGGGGGCCA ACAGCTGAGA GCTGGTTACC TGCCAAATCA 
CCCCCTTCAA GCAAAATTGG AAACAAGTCA ATCAATGCCA GCTGGCCCCC AGAGTTTCAG 
CCAGGAGTGC CATGGAAAGG GATCCAAAAT ATTGATCCAG AATCTGATCC TTATGTTACC 
CCTGGCAGTG TGCTTGGGGG TCCAGCCCCA TCTCCCATTG TAGATACTGA CCACCAGCTA 
CTGCAGGACA CCCCCACAGG GTCCAATTCT TCCCTCAACA CCTCGCTGCC TTCACCTGGT 
GCCTGGCCCT TTAGTGCCTC TGAGAATATC CATAGCACTT CAGCACTCTC TGCAAAGTTT 
GGGGACTACA AATCGACCTG GTCACCAGAC CCCATAGGAC ATAACCCATC ACATCACTCA 
AACAAGATGT GGAAAAACCA GATGTCCAGG AACAGCCCTG GGCTGCCTCG CCCACCTCCA 
GGGCTCACAA ACAACCCCAA AGCCACCAAC TCTCCCTGGA ACAGCACAGC ACCTCGCTCC 
GTCAGGGGCT GGGGCGGACA GGACTCCAGA ATGGCTTCTG CATCAGCTTG GAGTGATGGT 
GGATCTGTGC GCCCCAGCTA CTGGCTGATC CTTCTTAATC TCACACCACA GATCGATGGT 
TCCACCCTGC GAACGATCTG CATGCAGCAT GGCCCCCTGC TGACATTCCA TCTCAACCTT 
ACCCAGGGCA CAGCTCTAAT CCGATACAGC ACCAAACAGG AGGCAGCCAA GGCCCAGAGT 
GCCCTCCATA TGTGTGTTCT GGGTAATACA ACTATCCTGG CGGAGTTTGC AACGGATGAG 
GAGGTGAGCC GGTATCTTGC ACAAGCTCAG CCACCAGCTC CCTCTGCTCC AGGAGCAGGC 
TGGCAGCCTT TGGAAGTGGG ACCGAACCAC CCAGCTGAAC CAGTAGGGCC AGCCCTAAAC 
CTTTTTGGAG GAACCACAGG GCTAGGGCAA TGGAGCAGCG GCGGTGGTGG TAGTGACCTG 
TCAGGGGTTT CTCTCTGGGG TACTCCTAGC AGCTACACAT CCAGTCTGTG GGGTGTACCG 
AGCACAGAGG ACGCCCACAG GATGGGCAGC CCTGCCCCGT TACTACCCGG TGACCTTCTG 
GGAGGAGGGA CCGACTCGAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816931.2
CDS	366..5969
Translation	MREKHLKKAVPREKEQEREEQLMEDKKRKKEDKKKKEAAQKVTEQKTKVPEVTKPSSSQATAASLPGSSPSPPVNGGNNAKRVAVPNGQPPSTARYMPREVPPRFRCQQDHKVLLKRGQPPPASCMLPGGGAGTPPSTTAPGAGPGNAQPGAGTLLPGESGASTDSSSGAAATSNYANSTWGSGGPTSSGPTTNPPHAWDKVIVDGSDMEEWPCIAGVDAEPPSDNATDNNSASNSASEKGPPQGSTTSHKGRGGQGQQQQQQQPGSECIQGVWKSDPKAKPVPSSNPASEGVNRLGNWRNGGGSERAGSASGFSNFNPNTNPSAWPALVQEGNRKGNPESDSSFSGAQLGPVGLTSREQQSKMENAGSSFAGSGRESATTHHTDGPKNGNTNSTNSNTLNPPDNKGTAFGVGSMDSCRGSDSPAQSTGERKNGGIGSWGAGRGQSATGDANCGHSNSGSNGREREDARKAPSSHRPNGSRGESWESKSGNGSWGYSPQADLSEVKWSEGNKLGAGGASQGEWKQPAGHEDLKVGEWGGLNQGNSSTGAWDNQKGHSLSDNQGNTSSAACWGRAPSSAGSEAGGQSTGSNAKAGGSGGSGDSLNSSSRRSHRPALPDSQAALQTLLNRTDLDPRVLSNSGWGQTQIKQDASWESEEVPSRTEGKSDRGTEGWENVSQAKTSGGWGDAPSQSNQNKSGWGEPPVPAPSTQEWKDTKATGWNDYKNSPTGWVGGGGGGVVGARPEDKAVPGWNNESGGAAEPGWSTRQSNPGGGWSAGKNGWGGNGSEDMEPPKAPGGWEGSGSKSSSGWGEVGQNDVGSWGNGANSNPAPRTGWEETKRPQGGGNWGEAARPQLPWNKQQESTPSSATPSTWGGSQAPPPPGNGRSQPTSGWKGGPQPTVSKEEEPSGWEEPSSQSIGHKMDIDDGTAAWGDPNNYNYKNVSLWDKNAQAGPGQMAREQGISGTLANKAAPSVWSKNKGPPTDNGTSAWGEPTEPASGWGEGEESGPPTPGWGSAPQVNPSAMKSGTKSMQDGWGESDGPVTGTRHSSWDEEEEGGVWNSAGSQGSGSSHNSAGWGQGGKKPQIKGPLKGGSNDTWMNTLPKQFSNMGLLSQVDDPSTTKMDHPGGLPDKKFETDKRGVNLGDFNDMMRKERSGFRLNNSKEMGAADSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSSLPSVGLGAIGSGLNPQTFAARQGGNHGLFGNSGAQSRGMHNPVPSLNSSPNLRAQVPPQFLSPQVSASMLKQFPNSNMNPGLFNMGPQLSPQQIAMLSQLPQIQQFQLACQLLLQQQQQQQQQQQQQQLLQNQRKISQAVRQQQEQQQIARMVSALQQQQQQQQQQQQRQPGMKHSPSHPAGPKGHLDAVGLSDMQAKGHMPYGSGFGSSGMDYGTVGKEAGAESRFKQWTSMMEGLPSTVSQDTSLHKNGSIAPPVKNRGGSPYHQYDMLSTDALGGHGGPTAESWLPAKSPPSSKIGNKSINASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGPAPSPIVDTDHQLLQDTPTGSNSSLNTSLPSPGAWPFSASENIHSTSALSAKFGDYKSTWSPDPIGHNPSHHSNKMWKNQMSRNSPGLPRPPPGLTNNPKATNSPWNSTAPRSVRGWGGQDSRMASASAWSDGGSVRPSYWLILLNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQSALHMCVLGNTTILAEFATDEEVSRYLAQAQPPAPSAPGAGWQPLEVGPNHPAEPVGPALNLFGGTTGLGQWSSGGGGSDLSGVSLWGTPSSYTSSLWGVPSTEDAHRMGSPAPLLPGDLLGGGTDSI

Target ORF information:

RefSeq Version	XM_012961477.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961477.3

ORF Insert Sequence:

ATGAGGGAGA AACACTTGAA GAAAGCTGTT CCCAGAGAGA AGGAGCAAGA AAGGGAAGAA 
CAGTTAATGG AAGACAAGAA AAGGAAGAAA GAGGATAAAA AGAAGAAAGA AGCTGCTCAG 
AAGGTCACAG AACAAAAAAC CAAAGTGCCC GAAGTGACGA AACCAAGTTC AAGCCAAGCG 
ACGGCCGCCA GCCTACCTGG CAGCTCCCCC TCGCCACCAG TCAATGGTGG CAACAATGCC 
AAAAGGGTGG CAGTGCCGAA CGGACAACCG CCCAGCACCG CCCGCTATAT GCCTCGGGAG 
GTGCCGCCGC GATTCCGCTG CCAGCAGGAC CACAAAGTGT TACTGAAGCG TGGACAGCCT 
CCTCCAGCGT CCTGTATGCT GCCGGGGGGA GGAGCAGGGA CTCCCCCCTC AACCACTGCT 
CCAGGGGCCG GCCCAGGCAA CGCACAGCCA GGGGCGGGGA CACTGCTGCC AGGGGAAAGC 
GGGGCTTCAA CAGACTCATC ATCGGGGGCA GCTGCTACTT CAAATTATGC AAATTCCACT 
TGGGGCTCAG GAGGCCCCAC CAGCAGCGGC CCCACCACAA ACCCTCCCCA CGCCTGGGAC 
AAAGTGATTG TAGACGGGTC GGACATGGAA GAGTGGCCTT GCATAGCCGG CGTGGACGCA 
GAGCCCCCTT CCGACAACGC CACAGACAAC AACAGTGCCT CGAACTCAGC CTCGGAGAAG 
GGACCCCCAC AAGGAAGCAC CACTAGCCAC AAAGGGCGAG GGGGCCAGGG CCAGCAGCAA 
CAGCAGCAGC AGCCTGGTAG TGAATGTATC CAGGGCGTCT GGAAATCTGA CCCCAAGGCT 
AAGCCCGTCC CGTCTTCCAA CCCTGCTTCA GAGGGTGTAA ATAGACTGGG AAACTGGAGA 
AATGGGGGTG GGTCAGAGAG AGCAGGTTCT GCCTCTGGTT TCAGCAACTT TAATCCAAAT 
ACTAACCCAT CTGCTTGGCC TGCCCTTGTC CAGGAAGGCA ACAGGAAAGG GAACCCAGAG 
TCAGACAGCA GCTTTTCTGG CGCACAACTC GGCCCAGTGG GTTTGACCTC TCGGGAGCAG 
CAGTCAAAGA TGGAAAATGC AGGTTCCAGC TTTGCAGGAT CGGGCAGGGA GTCTGCCACA 
ACCCATCACA CTGACGGACC AAAAAATGGA AACACTAACT CTACGAACTC AAATACTTTG 
AACCCTCCTG ATAACAAGGG AACAGCTTTT GGGGTGGGCT CAATGGATTC CTGCCGGGGT 
TCAGACTCCC CTGCTCAAAG CACTGGAGAA AGAAAGAATG GGGGGATTGG CTCTTGGGGA 
GCGGGAAGGG GTCAGTCTGC CACAGGAGAT GCTAATTGTG GACATAGCAA CTCTGGGAGC 
AATGGACGAG AGAGAGAAGA CGCAAGGAAA GCGCCTTCAT CTCATAGACC CAATGGGTCA 
AGGGGGGAAT CTTGGGAAAG CAAAAGTGGG AATGGTTCTT GGGGTTACAG CCCGCAGGCA 
GACCTCAGTG AAGTGAAATG GAGTGAAGGG AACAAATTGG GGGCTGGAGG GGCGTCTCAG 
GGAGAATGGA AGCAGCCAGC CGGGCATGAG GACTTAAAGG TGGGAGAGTG GGGAGGCTTG 
AACCAAGGAA ACTCTAGCAC TGGAGCATGG GACAATCAGA AGGGCCACTC TCTATCGGAT 
AACCAGGGCA ACACGTCATC AGCAGCCTGC TGGGGCCGAG CCCCCAGCTC TGCAGGAAGT 
GAAGCTGGGG GGCAAAGCAC TGGAAGCAAC GCTAAAGCAG GGGGGAGCGG AGGAAGCGGA 
GACAGCCTGA ACTCCAGCAG TCGACGTTCA CACAGACCTG CCCTCCCAGA CAGTCAGGCT 
GCTCTTCAGA CTTTACTGAA TCGCACTGAT TTGGATCCCC GGGTGCTGTC CAACTCTGGC 
TGGGGCCAAA CCCAGATCAA GCAAGATGCA TCATGGGAGA GTGAGGAAGT GCCCTCTCGA 
ACTGAAGGCA AATCAGACCG TGGGACTGAA GGTTGGGAGA ACGTGTCCCA AGCCAAAACC 
TCAGGGGGTT GGGGGGATGC TCCCAGCCAA AGCAATCAAA ACAAGTCAGG CTGGGGGGAG 
CCCCCTGTCC CTGCACCTTC AACCCAAGAA TGGAAGGACA CCAAAGCAAC TGGCTGGAAT 
GACTATAAAA ACAGCCCAAC TGGTTGGGTT GGTGGAGGAG GTGGTGGGGT GGTTGGAGCA 
CGACCAGAGG ACAAGGCAGT TCCAGGTTGG AACAATGAAA GTGGTGGGGC TGCGGAGCCT 
GGGTGGAGTA CTCGCCAGTC TAATCCAGGA GGTGGCTGGT CAGCAGGAAA GAATGGTTGG 
GGGGGAAATG GTAGTGAGGA CATGGAGCCT CCCAAGGCCC CTGGAGGCTG GGAAGGCTCA 
GGAAGCAAAT CCTCCTCTGG CTGGGGTGAA GTTGGACAAA ATGATGTGGG TTCTTGGGGT 
AATGGGGCAA ACTCAAATCC AGCTCCACGA ACTGGCTGGG AGGAGACTAA GAGGCCCCAG 
GGAGGAGGAA ACTGGGGTGA GGCAGCTAGG CCCCAACTAC CCTGGAACAA ACAGCAGGAA 
TCAACGCCAT CTTCAGCAAC TCCTAGCACC TGGGGGGGCT CTCAGGCTCC CCCGCCACCT 
GGAAATGGGA GGTCACAGCC AACTTCAGGG TGGAAGGGTG GTCCCCAGCC AACTGTTTCC 
AAGGAGGAAG AGCCAAGTGG CTGGGAGGAA CCATCCTCAC AATCCATTGG TCATAAAATG 
GACATTGATG ACGGAACAGC AGCTTGGGGG GATCCTAACA ATTACAATTA CAAAAATGTC 
AGCCTGTGGG ACAAGAATGC TCAAGCAGGC CCTGGGCAGA TGGCTCGGGA GCAAGGAATA 
TCTGGGACAT TGGCAAACAA GGCAGCTCCC TCAGTGTGGA GTAAAAACAA AGGACCCCCA 
ACTGACAATG GGACATCAGC TTGGGGTGAG CCAACTGAAC CTGCTTCAGG GTGGGGAGAA 
GGTGAGGAGT CGGGTCCACC AACACCAGGA TGGGGAAGTG CACCCCAAGT AAATCCCAGT 
GCCATGAAAT CTGGTACAAA ATCTATGCAA GATGGCTGGG GTGAGAGTGA TGGGCCGGTG 
ACAGGAACAC GTCACAGCAG CTGGGATGAA GAAGAAGAGG GTGGAGTCTG GAACAGTGCA 
GGTTCTCAGG GAAGTGGCTC CTCCCACAAC TCTGCAGGTT GGGGACAAGG AGGAAAGAAA 
CCACAGATCA AGGGTCCCTT AAAAGGAGGG AGCAATGATA CCTGGATGAA CACCTTGCCC 
AAACAGTTCT CCAACATGGG ATTGTTGAGC CAAGTTGATG ATCCCTCTAC CACTAAGATG 
GACCATCCTG GCGGGCTTCC AGATAAGAAG TTTGAGACAG ACAAGCGGGG CGTGAATCTC 
GGGGATTTTA ATGACATGAT GCGGAAGGAA CGGTCTGGCT TCCGTCTGAA CAATTCCAAA 
GAGATGGGAG CTGCAGACAG TGGGCCTTAT TTTGAGAAGC TGACTTTGCC TTTCTCCAAT 
CAAGACGGGT GCCTTGGGGA TGAGGCTCCC TGTTCCCCTT TTTCTCCCTC CCCTAGCTAC 
AAGCTATCTC CCTCCGGCTC CTCGCTCCCT TCCGTGGGCC TGGGGGCTAT CGGTTCTGGC 
CTCAACCCCC AAACCTTCGC TGCTAGACAA GGTGGCAATC ACGGACTATT TGGAAACAGC 
GGTGCACAAT CGCGAGGCAT GCACAACCCT GTGCCATCGC TCAACTCCTC TCCCAACCTC 
CGGGCACAAG TGCCTCCCCA GTTCCTCTCT CCCCAGGTCT CAGCCTCCAT GCTAAAGCAG 
TTTCCTAACA GTAACATGAA TCCAGGACTT TTCAATATGG GGCCACAGCT TTCCCCTCAG 
CAGATTGCAA TGCTGAGCCA GCTTCCTCAA ATTCAGCAGT TCCAGCTGGC ATGTCAGCTC 
CTCTTGCAAC AACAACAACA GCAGCAACAG CAGCAGCAGC AGCAGCAACT CCTTCAGAAC 
CAGAGGAAGA TCTCACAAGC GGTCAGACAG CAGCAGGAAC AGCAGCAGAT AGCACGCATG 
GTAAGTGCCC TACAACAGCA GCAACAGCAA CAGCAGCAGC AGCAACAGAG GCAGCCAGGA 
ATGAAGCACT CTCCCTCTCA CCCTGCTGGA CCCAAAGGTC ACTTAGATGC TGTTGGACTC 
TCAGACATGC AAGCCAAAGG GCACATGCCA TATGGATCTG GTTTTGGCTC AAGTGGGATG 
GATTATGGTA CAGTAGGTAA GGAGGCGGGA GCAGAGTCTC GATTTAAACA GTGGACATCC 
ATGATGGAGG GGCTACCGTC CACTGTTTCT CAAGATACCA GCCTGCATAA AAATGGCTCC 
ATTGCACCCC CAGTAAAAAA TCGTGGAGGT TCCCCCTACC ACCAATATGA CATGTTGTCT 
ACGGATGCTT TGGGAGGGCA TGGGGGGCCA ACAGCTGAGA GCTGGTTACC TGCCAAATCA 
CCCCCTTCAA GCAAAATTGG AAACAAGTCA ATCAATGCCA GCTGGCCCCC AGAGTTTCAG 
CCAGGAGTGC CATGGAAAGG GATCCAAAAT ATTGATCCAG AATCTGATCC TTATGTTACC 
CCTGGCAGTG TGCTTGGGGG TCCAGCCCCA TCTCCCATTG TAGATACTGA CCACCAGCTA 
CTGCAGGACA CCCCCACAGG GTCCAATTCT TCCCTCAACA CCTCGCTGCC TTCACCTGGT 
GCCTGGCCCT TTAGTGCCTC TGAGAATATC CATAGCACTT CAGCACTCTC TGCAAAGTTT 
GGGGACTACA AATCGACCTG GTCACCAGAC CCCATAGGAC ATAACCCATC ACATCACTCA 
AACAAGATGT GGAAAAACCA GATGTCCAGG AACAGCCCTG GGCTGCCTCG CCCACCTCCA 
GGGCTCACAA ACAACCCCAA AGCCACCAAC TCTCCCTGGA ACAGCACAGC ACCTCGCTCC 
GTCAGGGGCT GGGGCGGACA GGACTCCAGA ATGGCTTCTG CATCAGCTTG GAGTGATGGT 
GGATCTGTGC GCCCCAGCTA CTGGCTGATC CTTCTTAATC TCACACCACA GATCGATGGT 
TCCACCCTGC GAACGATCTG CATGCAGCAT GGCCCCCTGC TGACATTCCA TCTCAACCTT 
ACCCAGGGCA CAGCTCTAAT CCGATACAGC ACCAAACAGG AGGCAGCCAA GGCCCAGAGT 
GCCCTCCATA TGTGTGTTCT GGGTAATACA ACTATCCTGG CGGAGTTTGC AACGGATGAG 
GAGGTGAGCC GGTATCTTGC ACAAGCTCAG CCACCAGCTC CCTCTGCTCC AGGAGCAGGC 
TGGCAGCCTT TGGAAGTGGG ACCGAACCAC CCAGCTGAAC CAGTAGGGCC AGCCCTAAAC 
CTTTTTGGAG GAACCACAGG GCTAGGGCAA TGGAGCAGCG GCGGTGGTGG TAGTGACCTG 
TCAGGGGTTT CTCTCTGGGG TACTCCTAGC AGCTACACAT CCAGTCTGTG GGGTGTACCG 
AGCACAGAGG ACGCCCACAG GATGGGCAGC CCTGCCCCGT TACTACCCGG TGACCTTCTG 
GGAGGAGGGA CCGACTCGAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnrc6b ( XM_002934209.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934255.3 Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X6, mRNA.

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CloneID	OXa57435
Clone ID Related Accession (Same CDS sequence)	XM_012961477.3 , XM_002934209.5 , XM_012961478.3
Accession Version	XM_002934209.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trinucleotide repeat-containing gene 6B protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934209.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAGA AACACTTGAA GAAAGCTGTT CCCAGAGAGA AGGAGCAAGA AAGGGAAGAA 
CAGTTAATGG AAGACAAGAA AAGGAAGAAA GAGGATAAAA AGAAGAAAGA AGCTGCTCAG 
AAGGTCACAG AACAAAAAAC CAAAGTGCCC GAAGTGACGA AACCAAGTTC AAGCCAAGCG 
ACGGCCGCCA GCCTACCTGG CAGCTCCCCC TCGCCACCAG TCAATGGTGG CAACAATGCC 
AAAAGGGTGG CAGTGCCGAA CGGACAACCG CCCAGCACCG CCCGCTATAT GCCTCGGGAG 
GTGCCGCCGC GATTCCGCTG CCAGCAGGAC CACAAAGTGT TACTGAAGCG TGGACAGCCT 
CCTCCAGCGT CCTGTATGCT GCCGGGGGGA GGAGCAGGGA CTCCCCCCTC AACCACTGCT 
CCAGGGGCCG GCCCAGGCAA CGCACAGCCA GGGGCGGGGA CACTGCTGCC AGGGGAAAGC 
GGGGCTTCAA CAGACTCATC ATCGGGGGCA GCTGCTACTT CAAATTATGC AAATTCCACT 
TGGGGCTCAG GAGGCCCCAC CAGCAGCGGC CCCACCACAA ACCCTCCCCA CGCCTGGGAC 
AAAGTGATTG TAGACGGGTC GGACATGGAA GAGTGGCCTT GCATAGCCGG CGTGGACGCA 
GAGCCCCCTT CCGACAACGC CACAGACAAC AACAGTGCCT CGAACTCAGC CTCGGAGAAG 
GGACCCCCAC AAGGAAGCAC CACTAGCCAC AAAGGGCGAG GGGGCCAGGG CCAGCAGCAA 
CAGCAGCAGC AGCCTGGTAG TGAATGTATC CAGGGCGTCT GGAAATCTGA CCCCAAGGCT 
AAGCCCGTCC CGTCTTCCAA CCCTGCTTCA GAGGGTGTAA ATAGACTGGG AAACTGGAGA 
AATGGGGGTG GGTCAGAGAG AGCAGGTTCT GCCTCTGGTT TCAGCAACTT TAATCCAAAT 
ACTAACCCAT CTGCTTGGCC TGCCCTTGTC CAGGAAGGCA ACAGGAAAGG GAACCCAGAG 
TCAGACAGCA GCTTTTCTGG CGCACAACTC GGCCCAGTGG GTTTGACCTC TCGGGAGCAG 
CAGTCAAAGA TGGAAAATGC AGGTTCCAGC TTTGCAGGAT CGGGCAGGGA GTCTGCCACA 
ACCCATCACA CTGACGGACC AAAAAATGGA AACACTAACT CTACGAACTC AAATACTTTG 
AACCCTCCTG ATAACAAGGG AACAGCTTTT GGGGTGGGCT CAATGGATTC CTGCCGGGGT 
TCAGACTCCC CTGCTCAAAG CACTGGAGAA AGAAAGAATG GGGGGATTGG CTCTTGGGGA 
GCGGGAAGGG GTCAGTCTGC CACAGGAGAT GCTAATTGTG GACATAGCAA CTCTGGGAGC 
AATGGACGAG AGAGAGAAGA CGCAAGGAAA GCGCCTTCAT CTCATAGACC CAATGGGTCA 
AGGGGGGAAT CTTGGGAAAG CAAAAGTGGG AATGGTTCTT GGGGTTACAG CCCGCAGGCA 
GACCTCAGTG AAGTGAAATG GAGTGAAGGG AACAAATTGG GGGCTGGAGG GGCGTCTCAG 
GGAGAATGGA AGCAGCCAGC CGGGCATGAG GACTTAAAGG TGGGAGAGTG GGGAGGCTTG 
AACCAAGGAA ACTCTAGCAC TGGAGCATGG GACAATCAGA AGGGCCACTC TCTATCGGAT 
AACCAGGGCA ACACGTCATC AGCAGCCTGC TGGGGCCGAG CCCCCAGCTC TGCAGGAAGT 
GAAGCTGGGG GGCAAAGCAC TGGAAGCAAC GCTAAAGCAG GGGGGAGCGG AGGAAGCGGA 
GACAGCCTGA ACTCCAGCAG TCGACGTTCA CACAGACCTG CCCTCCCAGA CAGTCAGGCT 
GCTCTTCAGA CTTTACTGAA TCGCACTGAT TTGGATCCCC GGGTGCTGTC CAACTCTGGC 
TGGGGCCAAA CCCAGATCAA GCAAGATGCA TCATGGGAGA GTGAGGAAGT GCCCTCTCGA 
ACTGAAGGCA AATCAGACCG TGGGACTGAA GGTTGGGAGA ACGTGTCCCA AGCCAAAACC 
TCAGGGGGTT GGGGGGATGC TCCCAGCCAA AGCAATCAAA ACAAGTCAGG CTGGGGGGAG 
CCCCCTGTCC CTGCACCTTC AACCCAAGAA TGGAAGGACA CCAAAGCAAC TGGCTGGAAT 
GACTATAAAA ACAGCCCAAC TGGTTGGGTT GGTGGAGGAG GTGGTGGGGT GGTTGGAGCA 
CGACCAGAGG ACAAGGCAGT TCCAGGTTGG AACAATGAAA GTGGTGGGGC TGCGGAGCCT 
GGGTGGAGTA CTCGCCAGTC TAATCCAGGA GGTGGCTGGT CAGCAGGAAA GAATGGTTGG 
GGGGGAAATG GTAGTGAGGA CATGGAGCCT CCCAAGGCCC CTGGAGGCTG GGAAGGCTCA 
GGAAGCAAAT CCTCCTCTGG CTGGGGTGAA GTTGGACAAA ATGATGTGGG TTCTTGGGGT 
AATGGGGCAA ACTCAAATCC AGCTCCACGA ACTGGCTGGG AGGAGACTAA GAGGCCCCAG 
GGAGGAGGAA ACTGGGGTGA GGCAGCTAGG CCCCAACTAC CCTGGAACAA ACAGCAGGAA 
TCAACGCCAT CTTCAGCAAC TCCTAGCACC TGGGGGGGCT CTCAGGCTCC CCCGCCACCT 
GGAAATGGGA GGTCACAGCC AACTTCAGGG TGGAAGGGTG GTCCCCAGCC AACTGTTTCC 
AAGGAGGAAG AGCCAAGTGG CTGGGAGGAA CCATCCTCAC AATCCATTGG TCATAAAATG 
GACATTGATG ACGGAACAGC AGCTTGGGGG GATCCTAACA ATTACAATTA CAAAAATGTC 
AGCCTGTGGG ACAAGAATGC TCAAGCAGGC CCTGGGCAGA TGGCTCGGGA GCAAGGAATA 
TCTGGGACAT TGGCAAACAA GGCAGCTCCC TCAGTGTGGA GTAAAAACAA AGGACCCCCA 
ACTGACAATG GGACATCAGC TTGGGGTGAG CCAACTGAAC CTGCTTCAGG GTGGGGAGAA 
GGTGAGGAGT CGGGTCCACC AACACCAGGA TGGGGAAGTG CACCCCAAGT AAATCCCAGT 
GCCATGAAAT CTGGTACAAA ATCTATGCAA GATGGCTGGG GTGAGAGTGA TGGGCCGGTG 
ACAGGAACAC GTCACAGCAG CTGGGATGAA GAAGAAGAGG GTGGAGTCTG GAACAGTGCA 
GGTTCTCAGG GAAGTGGCTC CTCCCACAAC TCTGCAGGTT GGGGACAAGG AGGAAAGAAA 
CCACAGATCA AGGGTCCCTT AAAAGGAGGG AGCAATGATA CCTGGATGAA CACCTTGCCC 
AAACAGTTCT CCAACATGGG ATTGTTGAGC CAAGTTGATG ATCCCTCTAC CACTAAGATG 
GACCATCCTG GCGGGCTTCC AGATAAGAAG TTTGAGACAG ACAAGCGGGG CGTGAATCTC 
GGGGATTTTA ATGACATGAT GCGGAAGGAA CGGTCTGGCT TCCGTCTGAA CAATTCCAAA 
GAGATGGGAG CTGCAGACAG TGGGCCTTAT TTTGAGAAGC TGACTTTGCC TTTCTCCAAT 
CAAGACGGGT GCCTTGGGGA TGAGGCTCCC TGTTCCCCTT TTTCTCCCTC CCCTAGCTAC 
AAGCTATCTC CCTCCGGCTC CTCGCTCCCT TCCGTGGGCC TGGGGGCTAT CGGTTCTGGC 
CTCAACCCCC AAACCTTCGC TGCTAGACAA GGTGGCAATC ACGGACTATT TGGAAACAGC 
GGTGCACAAT CGCGAGGCAT GCACAACCCT GTGCCATCGC TCAACTCCTC TCCCAACCTC 
CGGGCACAAG TGCCTCCCCA GTTCCTCTCT CCCCAGGTCT CAGCCTCCAT GCTAAAGCAG 
TTTCCTAACA GTAACATGAA TCCAGGACTT TTCAATATGG GGCCACAGCT TTCCCCTCAG 
CAGATTGCAA TGCTGAGCCA GCTTCCTCAA ATTCAGCAGT TCCAGCTGGC ATGTCAGCTC 
CTCTTGCAAC AACAACAACA GCAGCAACAG CAGCAGCAGC AGCAGCAACT CCTTCAGAAC 
CAGAGGAAGA TCTCACAAGC GGTCAGACAG CAGCAGGAAC AGCAGCAGAT AGCACGCATG 
GTAAGTGCCC TACAACAGCA GCAACAGCAA CAGCAGCAGC AGCAACAGAG GCAGCCAGGA 
ATGAAGCACT CTCCCTCTCA CCCTGCTGGA CCCAAAGGTC ACTTAGATGC TGTTGGACTC 
TCAGACATGC AAGCCAAAGG GCACATGCCA TATGGATCTG GTTTTGGCTC AAGTGGGATG 
GATTATGGTA CAGTAGGTAA GGAGGCGGGA GCAGAGTCTC GATTTAAACA GTGGACATCC 
ATGATGGAGG GGCTACCGTC CACTGTTTCT CAAGATACCA GCCTGCATAA AAATGGCTCC 
ATTGCACCCC CAGTAAAAAA TCGTGGAGGT TCCCCCTACC ACCAATATGA CATGTTGTCT 
ACGGATGCTT TGGGAGGGCA TGGGGGGCCA ACAGCTGAGA GCTGGTTACC TGCCAAATCA 
CCCCCTTCAA GCAAAATTGG AAACAAGTCA ATCAATGCCA GCTGGCCCCC AGAGTTTCAG 
CCAGGAGTGC CATGGAAAGG GATCCAAAAT ATTGATCCAG AATCTGATCC TTATGTTACC 
CCTGGCAGTG TGCTTGGGGG TCCAGCCCCA TCTCCCATTG TAGATACTGA CCACCAGCTA 
CTGCAGGACA CCCCCACAGG GTCCAATTCT TCCCTCAACA CCTCGCTGCC TTCACCTGGT 
GCCTGGCCCT TTAGTGCCTC TGAGAATATC CATAGCACTT CAGCACTCTC TGCAAAGTTT 
GGGGACTACA AATCGACCTG GTCACCAGAC CCCATAGGAC ATAACCCATC ACATCACTCA 
AACAAGATGT GGAAAAACCA GATGTCCAGG AACAGCCCTG GGCTGCCTCG CCCACCTCCA 
GGGCTCACAA ACAACCCCAA AGCCACCAAC TCTCCCTGGA ACAGCACAGC ACCTCGCTCC 
GTCAGGGGCT GGGGCGGACA GGACTCCAGA ATGGCTTCTG CATCAGCTTG GAGTGATGGT 
GGATCTGTGC GCCCCAGCTA CTGGCTGATC CTTCTTAATC TCACACCACA GATCGATGGT 
TCCACCCTGC GAACGATCTG CATGCAGCAT GGCCCCCTGC TGACATTCCA TCTCAACCTT 
ACCCAGGGCA CAGCTCTAAT CCGATACAGC ACCAAACAGG AGGCAGCCAA GGCCCAGAGT 
GCCCTCCATA TGTGTGTTCT GGGTAATACA ACTATCCTGG CGGAGTTTGC AACGGATGAG 
GAGGTGAGCC GGTATCTTGC ACAAGCTCAG CCACCAGCTC CCTCTGCTCC AGGAGCAGGC 
TGGCAGCCTT TGGAAGTGGG ACCGAACCAC CCAGCTGAAC CAGTAGGGCC AGCCCTAAAC 
CTTTTTGGAG GAACCACAGG GCTAGGGCAA TGGAGCAGCG GCGGTGGTGG TAGTGACCTG 
TCAGGGGTTT CTCTCTGGGG TACTCCTAGC AGCTACACAT CCAGTCTGTG GGGTGTACCG 
AGCACAGAGG ACGCCCACAG GATGGGCAGC CCTGCCCCGT TACTACCCGG TGACCTTCTG 
GGAGGAGGGA CCGACTCGAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934255.3
CDS	392..5995
Translation	MREKHLKKAVPREKEQEREEQLMEDKKRKKEDKKKKEAAQKVTEQKTKVPEVTKPSSSQATAASLPGSSPSPPVNGGNNAKRVAVPNGQPPSTARYMPREVPPRFRCQQDHKVLLKRGQPPPASCMLPGGGAGTPPSTTAPGAGPGNAQPGAGTLLPGESGASTDSSSGAAATSNYANSTWGSGGPTSSGPTTNPPHAWDKVIVDGSDMEEWPCIAGVDAEPPSDNATDNNSASNSASEKGPPQGSTTSHKGRGGQGQQQQQQQPGSECIQGVWKSDPKAKPVPSSNPASEGVNRLGNWRNGGGSERAGSASGFSNFNPNTNPSAWPALVQEGNRKGNPESDSSFSGAQLGPVGLTSREQQSKMENAGSSFAGSGRESATTHHTDGPKNGNTNSTNSNTLNPPDNKGTAFGVGSMDSCRGSDSPAQSTGERKNGGIGSWGAGRGQSATGDANCGHSNSGSNGREREDARKAPSSHRPNGSRGESWESKSGNGSWGYSPQADLSEVKWSEGNKLGAGGASQGEWKQPAGHEDLKVGEWGGLNQGNSSTGAWDNQKGHSLSDNQGNTSSAACWGRAPSSAGSEAGGQSTGSNAKAGGSGGSGDSLNSSSRRSHRPALPDSQAALQTLLNRTDLDPRVLSNSGWGQTQIKQDASWESEEVPSRTEGKSDRGTEGWENVSQAKTSGGWGDAPSQSNQNKSGWGEPPVPAPSTQEWKDTKATGWNDYKNSPTGWVGGGGGGVVGARPEDKAVPGWNNESGGAAEPGWSTRQSNPGGGWSAGKNGWGGNGSEDMEPPKAPGGWEGSGSKSSSGWGEVGQNDVGSWGNGANSNPAPRTGWEETKRPQGGGNWGEAARPQLPWNKQQESTPSSATPSTWGGSQAPPPPGNGRSQPTSGWKGGPQPTVSKEEEPSGWEEPSSQSIGHKMDIDDGTAAWGDPNNYNYKNVSLWDKNAQAGPGQMAREQGISGTLANKAAPSVWSKNKGPPTDNGTSAWGEPTEPASGWGEGEESGPPTPGWGSAPQVNPSAMKSGTKSMQDGWGESDGPVTGTRHSSWDEEEEGGVWNSAGSQGSGSSHNSAGWGQGGKKPQIKGPLKGGSNDTWMNTLPKQFSNMGLLSQVDDPSTTKMDHPGGLPDKKFETDKRGVNLGDFNDMMRKERSGFRLNNSKEMGAADSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSSLPSVGLGAIGSGLNPQTFAARQGGNHGLFGNSGAQSRGMHNPVPSLNSSPNLRAQVPPQFLSPQVSASMLKQFPNSNMNPGLFNMGPQLSPQQIAMLSQLPQIQQFQLACQLLLQQQQQQQQQQQQQQLLQNQRKISQAVRQQQEQQQIARMVSALQQQQQQQQQQQQRQPGMKHSPSHPAGPKGHLDAVGLSDMQAKGHMPYGSGFGSSGMDYGTVGKEAGAESRFKQWTSMMEGLPSTVSQDTSLHKNGSIAPPVKNRGGSPYHQYDMLSTDALGGHGGPTAESWLPAKSPPSSKIGNKSINASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGPAPSPIVDTDHQLLQDTPTGSNSSLNTSLPSPGAWPFSASENIHSTSALSAKFGDYKSTWSPDPIGHNPSHHSNKMWKNQMSRNSPGLPRPPPGLTNNPKATNSPWNSTAPRSVRGWGGQDSRMASASAWSDGGSVRPSYWLILLNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQSALHMCVLGNTTILAEFATDEEVSRYLAQAQPPAPSAPGAGWQPLEVGPNHPAEPVGPALNLFGGTTGLGQWSSGGGGSDLSGVSLWGTPSSYTSSLWGVPSTEDAHRMGSPAPLLPGDLLGGGTDSI

Target ORF information:

RefSeq Version	XM_002934209.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934209.5

ORF Insert Sequence:

ATGAGGGAGA AACACTTGAA GAAAGCTGTT CCCAGAGAGA AGGAGCAAGA AAGGGAAGAA 
CAGTTAATGG AAGACAAGAA AAGGAAGAAA GAGGATAAAA AGAAGAAAGA AGCTGCTCAG 
AAGGTCACAG AACAAAAAAC CAAAGTGCCC GAAGTGACGA AACCAAGTTC AAGCCAAGCG 
ACGGCCGCCA GCCTACCTGG CAGCTCCCCC TCGCCACCAG TCAATGGTGG CAACAATGCC 
AAAAGGGTGG CAGTGCCGAA CGGACAACCG CCCAGCACCG CCCGCTATAT GCCTCGGGAG 
GTGCCGCCGC GATTCCGCTG CCAGCAGGAC CACAAAGTGT TACTGAAGCG TGGACAGCCT 
CCTCCAGCGT CCTGTATGCT GCCGGGGGGA GGAGCAGGGA CTCCCCCCTC AACCACTGCT 
CCAGGGGCCG GCCCAGGCAA CGCACAGCCA GGGGCGGGGA CACTGCTGCC AGGGGAAAGC 
GGGGCTTCAA CAGACTCATC ATCGGGGGCA GCTGCTACTT CAAATTATGC AAATTCCACT 
TGGGGCTCAG GAGGCCCCAC CAGCAGCGGC CCCACCACAA ACCCTCCCCA CGCCTGGGAC 
AAAGTGATTG TAGACGGGTC GGACATGGAA GAGTGGCCTT GCATAGCCGG CGTGGACGCA 
GAGCCCCCTT CCGACAACGC CACAGACAAC AACAGTGCCT CGAACTCAGC CTCGGAGAAG 
GGACCCCCAC AAGGAAGCAC CACTAGCCAC AAAGGGCGAG GGGGCCAGGG CCAGCAGCAA 
CAGCAGCAGC AGCCTGGTAG TGAATGTATC CAGGGCGTCT GGAAATCTGA CCCCAAGGCT 
AAGCCCGTCC CGTCTTCCAA CCCTGCTTCA GAGGGTGTAA ATAGACTGGG AAACTGGAGA 
AATGGGGGTG GGTCAGAGAG AGCAGGTTCT GCCTCTGGTT TCAGCAACTT TAATCCAAAT 
ACTAACCCAT CTGCTTGGCC TGCCCTTGTC CAGGAAGGCA ACAGGAAAGG GAACCCAGAG 
TCAGACAGCA GCTTTTCTGG CGCACAACTC GGCCCAGTGG GTTTGACCTC TCGGGAGCAG 
CAGTCAAAGA TGGAAAATGC AGGTTCCAGC TTTGCAGGAT CGGGCAGGGA GTCTGCCACA 
ACCCATCACA CTGACGGACC AAAAAATGGA AACACTAACT CTACGAACTC AAATACTTTG 
AACCCTCCTG ATAACAAGGG AACAGCTTTT GGGGTGGGCT CAATGGATTC CTGCCGGGGT 
TCAGACTCCC CTGCTCAAAG CACTGGAGAA AGAAAGAATG GGGGGATTGG CTCTTGGGGA 
GCGGGAAGGG GTCAGTCTGC CACAGGAGAT GCTAATTGTG GACATAGCAA CTCTGGGAGC 
AATGGACGAG AGAGAGAAGA CGCAAGGAAA GCGCCTTCAT CTCATAGACC CAATGGGTCA 
AGGGGGGAAT CTTGGGAAAG CAAAAGTGGG AATGGTTCTT GGGGTTACAG CCCGCAGGCA 
GACCTCAGTG AAGTGAAATG GAGTGAAGGG AACAAATTGG GGGCTGGAGG GGCGTCTCAG 
GGAGAATGGA AGCAGCCAGC CGGGCATGAG GACTTAAAGG TGGGAGAGTG GGGAGGCTTG 
AACCAAGGAA ACTCTAGCAC TGGAGCATGG GACAATCAGA AGGGCCACTC TCTATCGGAT 
AACCAGGGCA ACACGTCATC AGCAGCCTGC TGGGGCCGAG CCCCCAGCTC TGCAGGAAGT 
GAAGCTGGGG GGCAAAGCAC TGGAAGCAAC GCTAAAGCAG GGGGGAGCGG AGGAAGCGGA 
GACAGCCTGA ACTCCAGCAG TCGACGTTCA CACAGACCTG CCCTCCCAGA CAGTCAGGCT 
GCTCTTCAGA CTTTACTGAA TCGCACTGAT TTGGATCCCC GGGTGCTGTC CAACTCTGGC 
TGGGGCCAAA CCCAGATCAA GCAAGATGCA TCATGGGAGA GTGAGGAAGT GCCCTCTCGA 
ACTGAAGGCA AATCAGACCG TGGGACTGAA GGTTGGGAGA ACGTGTCCCA AGCCAAAACC 
TCAGGGGGTT GGGGGGATGC TCCCAGCCAA AGCAATCAAA ACAAGTCAGG CTGGGGGGAG 
CCCCCTGTCC CTGCACCTTC AACCCAAGAA TGGAAGGACA CCAAAGCAAC TGGCTGGAAT 
GACTATAAAA ACAGCCCAAC TGGTTGGGTT GGTGGAGGAG GTGGTGGGGT GGTTGGAGCA 
CGACCAGAGG ACAAGGCAGT TCCAGGTTGG AACAATGAAA GTGGTGGGGC TGCGGAGCCT 
GGGTGGAGTA CTCGCCAGTC TAATCCAGGA GGTGGCTGGT CAGCAGGAAA GAATGGTTGG 
GGGGGAAATG GTAGTGAGGA CATGGAGCCT CCCAAGGCCC CTGGAGGCTG GGAAGGCTCA 
GGAAGCAAAT CCTCCTCTGG CTGGGGTGAA GTTGGACAAA ATGATGTGGG TTCTTGGGGT 
AATGGGGCAA ACTCAAATCC AGCTCCACGA ACTGGCTGGG AGGAGACTAA GAGGCCCCAG 
GGAGGAGGAA ACTGGGGTGA GGCAGCTAGG CCCCAACTAC CCTGGAACAA ACAGCAGGAA 
TCAACGCCAT CTTCAGCAAC TCCTAGCACC TGGGGGGGCT CTCAGGCTCC CCCGCCACCT 
GGAAATGGGA GGTCACAGCC AACTTCAGGG TGGAAGGGTG GTCCCCAGCC AACTGTTTCC 
AAGGAGGAAG AGCCAAGTGG CTGGGAGGAA CCATCCTCAC AATCCATTGG TCATAAAATG 
GACATTGATG ACGGAACAGC AGCTTGGGGG GATCCTAACA ATTACAATTA CAAAAATGTC 
AGCCTGTGGG ACAAGAATGC TCAAGCAGGC CCTGGGCAGA TGGCTCGGGA GCAAGGAATA 
TCTGGGACAT TGGCAAACAA GGCAGCTCCC TCAGTGTGGA GTAAAAACAA AGGACCCCCA 
ACTGACAATG GGACATCAGC TTGGGGTGAG CCAACTGAAC CTGCTTCAGG GTGGGGAGAA 
GGTGAGGAGT CGGGTCCACC AACACCAGGA TGGGGAAGTG CACCCCAAGT AAATCCCAGT 
GCCATGAAAT CTGGTACAAA ATCTATGCAA GATGGCTGGG GTGAGAGTGA TGGGCCGGTG 
ACAGGAACAC GTCACAGCAG CTGGGATGAA GAAGAAGAGG GTGGAGTCTG GAACAGTGCA 
GGTTCTCAGG GAAGTGGCTC CTCCCACAAC TCTGCAGGTT GGGGACAAGG AGGAAAGAAA 
CCACAGATCA AGGGTCCCTT AAAAGGAGGG AGCAATGATA CCTGGATGAA CACCTTGCCC 
AAACAGTTCT CCAACATGGG ATTGTTGAGC CAAGTTGATG ATCCCTCTAC CACTAAGATG 
GACCATCCTG GCGGGCTTCC AGATAAGAAG TTTGAGACAG ACAAGCGGGG CGTGAATCTC 
GGGGATTTTA ATGACATGAT GCGGAAGGAA CGGTCTGGCT TCCGTCTGAA CAATTCCAAA 
GAGATGGGAG CTGCAGACAG TGGGCCTTAT TTTGAGAAGC TGACTTTGCC TTTCTCCAAT 
CAAGACGGGT GCCTTGGGGA TGAGGCTCCC TGTTCCCCTT TTTCTCCCTC CCCTAGCTAC 
AAGCTATCTC CCTCCGGCTC CTCGCTCCCT TCCGTGGGCC TGGGGGCTAT CGGTTCTGGC 
CTCAACCCCC AAACCTTCGC TGCTAGACAA GGTGGCAATC ACGGACTATT TGGAAACAGC 
GGTGCACAAT CGCGAGGCAT GCACAACCCT GTGCCATCGC TCAACTCCTC TCCCAACCTC 
CGGGCACAAG TGCCTCCCCA GTTCCTCTCT CCCCAGGTCT CAGCCTCCAT GCTAAAGCAG 
TTTCCTAACA GTAACATGAA TCCAGGACTT TTCAATATGG GGCCACAGCT TTCCCCTCAG 
CAGATTGCAA TGCTGAGCCA GCTTCCTCAA ATTCAGCAGT TCCAGCTGGC ATGTCAGCTC 
CTCTTGCAAC AACAACAACA GCAGCAACAG CAGCAGCAGC AGCAGCAACT CCTTCAGAAC 
CAGAGGAAGA TCTCACAAGC GGTCAGACAG CAGCAGGAAC AGCAGCAGAT AGCACGCATG 
GTAAGTGCCC TACAACAGCA GCAACAGCAA CAGCAGCAGC AGCAACAGAG GCAGCCAGGA 
ATGAAGCACT CTCCCTCTCA CCCTGCTGGA CCCAAAGGTC ACTTAGATGC TGTTGGACTC 
TCAGACATGC AAGCCAAAGG GCACATGCCA TATGGATCTG GTTTTGGCTC AAGTGGGATG 
GATTATGGTA CAGTAGGTAA GGAGGCGGGA GCAGAGTCTC GATTTAAACA GTGGACATCC 
ATGATGGAGG GGCTACCGTC CACTGTTTCT CAAGATACCA GCCTGCATAA AAATGGCTCC 
ATTGCACCCC CAGTAAAAAA TCGTGGAGGT TCCCCCTACC ACCAATATGA CATGTTGTCT 
ACGGATGCTT TGGGAGGGCA TGGGGGGCCA ACAGCTGAGA GCTGGTTACC TGCCAAATCA 
CCCCCTTCAA GCAAAATTGG AAACAAGTCA ATCAATGCCA GCTGGCCCCC AGAGTTTCAG 
CCAGGAGTGC CATGGAAAGG GATCCAAAAT ATTGATCCAG AATCTGATCC TTATGTTACC 
CCTGGCAGTG TGCTTGGGGG TCCAGCCCCA TCTCCCATTG TAGATACTGA CCACCAGCTA 
CTGCAGGACA CCCCCACAGG GTCCAATTCT TCCCTCAACA CCTCGCTGCC TTCACCTGGT 
GCCTGGCCCT TTAGTGCCTC TGAGAATATC CATAGCACTT CAGCACTCTC TGCAAAGTTT 
GGGGACTACA AATCGACCTG GTCACCAGAC CCCATAGGAC ATAACCCATC ACATCACTCA 
AACAAGATGT GGAAAAACCA GATGTCCAGG AACAGCCCTG GGCTGCCTCG CCCACCTCCA 
GGGCTCACAA ACAACCCCAA AGCCACCAAC TCTCCCTGGA ACAGCACAGC ACCTCGCTCC 
GTCAGGGGCT GGGGCGGACA GGACTCCAGA ATGGCTTCTG CATCAGCTTG GAGTGATGGT 
GGATCTGTGC GCCCCAGCTA CTGGCTGATC CTTCTTAATC TCACACCACA GATCGATGGT 
TCCACCCTGC GAACGATCTG CATGCAGCAT GGCCCCCTGC TGACATTCCA TCTCAACCTT 
ACCCAGGGCA CAGCTCTAAT CCGATACAGC ACCAAACAGG AGGCAGCCAA GGCCCAGAGT 
GCCCTCCATA TGTGTGTTCT GGGTAATACA ACTATCCTGG CGGAGTTTGC AACGGATGAG 
GAGGTGAGCC GGTATCTTGC ACAAGCTCAG CCACCAGCTC CCTCTGCTCC AGGAGCAGGC 
TGGCAGCCTT TGGAAGTGGG ACCGAACCAC CCAGCTGAAC CAGTAGGGCC AGCCCTAAAC 
CTTTTTGGAG GAACCACAGG GCTAGGGCAA TGGAGCAGCG GCGGTGGTGG TAGTGACCTG 
TCAGGGGTTT CTCTCTGGGG TACTCCTAGC AGCTACACAT CCAGTCTGTG GGGTGTACCG 
AGCACAGAGG ACGCCCACAG GATGGGCAGC CCTGCCCCGT TACTACCCGG TGACCTTCTG 
GGAGGAGGGA CCGACTCGAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnrc6b ( XM_012961478.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816932.2 Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X5, mRNA.

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CloneID	OXa57435
Clone ID Related Accession (Same CDS sequence)	XM_012961477.3 , XM_002934209.5 , XM_012961478.3
Accession Version	XM_012961478.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trinucleotide repeat-containing gene 6B protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961478.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAGA AACACTTGAA GAAAGCTGTT CCCAGAGAGA AGGAGCAAGA AAGGGAAGAA 
CAGTTAATGG AAGACAAGAA AAGGAAGAAA GAGGATAAAA AGAAGAAAGA AGCTGCTCAG 
AAGGTCACAG AACAAAAAAC CAAAGTGCCC GAAGTGACGA AACCAAGTTC AAGCCAAGCG 
ACGGCCGCCA GCCTACCTGG CAGCTCCCCC TCGCCACCAG TCAATGGTGG CAACAATGCC 
AAAAGGGTGG CAGTGCCGAA CGGACAACCG CCCAGCACCG CCCGCTATAT GCCTCGGGAG 
GTGCCGCCGC GATTCCGCTG CCAGCAGGAC CACAAAGTGT TACTGAAGCG TGGACAGCCT 
CCTCCAGCGT CCTGTATGCT GCCGGGGGGA GGAGCAGGGA CTCCCCCCTC AACCACTGCT 
CCAGGGGCCG GCCCAGGCAA CGCACAGCCA GGGGCGGGGA CACTGCTGCC AGGGGAAAGC 
GGGGCTTCAA CAGACTCATC ATCGGGGGCA GCTGCTACTT CAAATTATGC AAATTCCACT 
TGGGGCTCAG GAGGCCCCAC CAGCAGCGGC CCCACCACAA ACCCTCCCCA CGCCTGGGAC 
AAAGTGATTG TAGACGGGTC GGACATGGAA GAGTGGCCTT GCATAGCCGG CGTGGACGCA 
GAGCCCCCTT CCGACAACGC CACAGACAAC AACAGTGCCT CGAACTCAGC CTCGGAGAAG 
GGACCCCCAC AAGGAAGCAC CACTAGCCAC AAAGGGCGAG GGGGCCAGGG CCAGCAGCAA 
CAGCAGCAGC AGCCTGGTAG TGAATGTATC CAGGGCGTCT GGAAATCTGA CCCCAAGGCT 
AAGCCCGTCC CGTCTTCCAA CCCTGCTTCA GAGGGTGTAA ATAGACTGGG AAACTGGAGA 
AATGGGGGTG GGTCAGAGAG AGCAGGTTCT GCCTCTGGTT TCAGCAACTT TAATCCAAAT 
ACTAACCCAT CTGCTTGGCC TGCCCTTGTC CAGGAAGGCA ACAGGAAAGG GAACCCAGAG 
TCAGACAGCA GCTTTTCTGG CGCACAACTC GGCCCAGTGG GTTTGACCTC TCGGGAGCAG 
CAGTCAAAGA TGGAAAATGC AGGTTCCAGC TTTGCAGGAT CGGGCAGGGA GTCTGCCACA 
ACCCATCACA CTGACGGACC AAAAAATGGA AACACTAACT CTACGAACTC AAATACTTTG 
AACCCTCCTG ATAACAAGGG AACAGCTTTT GGGGTGGGCT CAATGGATTC CTGCCGGGGT 
TCAGACTCCC CTGCTCAAAG CACTGGAGAA AGAAAGAATG GGGGGATTGG CTCTTGGGGA 
GCGGGAAGGG GTCAGTCTGC CACAGGAGAT GCTAATTGTG GACATAGCAA CTCTGGGAGC 
AATGGACGAG AGAGAGAAGA CGCAAGGAAA GCGCCTTCAT CTCATAGACC CAATGGGTCA 
AGGGGGGAAT CTTGGGAAAG CAAAAGTGGG AATGGTTCTT GGGGTTACAG CCCGCAGGCA 
GACCTCAGTG AAGTGAAATG GAGTGAAGGG AACAAATTGG GGGCTGGAGG GGCGTCTCAG 
GGAGAATGGA AGCAGCCAGC CGGGCATGAG GACTTAAAGG TGGGAGAGTG GGGAGGCTTG 
AACCAAGGAA ACTCTAGCAC TGGAGCATGG GACAATCAGA AGGGCCACTC TCTATCGGAT 
AACCAGGGCA ACACGTCATC AGCAGCCTGC TGGGGCCGAG CCCCCAGCTC TGCAGGAAGT 
GAAGCTGGGG GGCAAAGCAC TGGAAGCAAC GCTAAAGCAG GGGGGAGCGG AGGAAGCGGA 
GACAGCCTGA ACTCCAGCAG TCGACGTTCA CACAGACCTG CCCTCCCAGA CAGTCAGGCT 
GCTCTTCAGA CTTTACTGAA TCGCACTGAT TTGGATCCCC GGGTGCTGTC CAACTCTGGC 
TGGGGCCAAA CCCAGATCAA GCAAGATGCA TCATGGGAGA GTGAGGAAGT GCCCTCTCGA 
ACTGAAGGCA AATCAGACCG TGGGACTGAA GGTTGGGAGA ACGTGTCCCA AGCCAAAACC 
TCAGGGGGTT GGGGGGATGC TCCCAGCCAA AGCAATCAAA ACAAGTCAGG CTGGGGGGAG 
CCCCCTGTCC CTGCACCTTC AACCCAAGAA TGGAAGGACA CCAAAGCAAC TGGCTGGAAT 
GACTATAAAA ACAGCCCAAC TGGTTGGGTT GGTGGAGGAG GTGGTGGGGT GGTTGGAGCA 
CGACCAGAGG ACAAGGCAGT TCCAGGTTGG AACAATGAAA GTGGTGGGGC TGCGGAGCCT 
GGGTGGAGTA CTCGCCAGTC TAATCCAGGA GGTGGCTGGT CAGCAGGAAA GAATGGTTGG 
GGGGGAAATG GTAGTGAGGA CATGGAGCCT CCCAAGGCCC CTGGAGGCTG GGAAGGCTCA 
GGAAGCAAAT CCTCCTCTGG CTGGGGTGAA GTTGGACAAA ATGATGTGGG TTCTTGGGGT 
AATGGGGCAA ACTCAAATCC AGCTCCACGA ACTGGCTGGG AGGAGACTAA GAGGCCCCAG 
GGAGGAGGAA ACTGGGGTGA GGCAGCTAGG CCCCAACTAC CCTGGAACAA ACAGCAGGAA 
TCAACGCCAT CTTCAGCAAC TCCTAGCACC TGGGGGGGCT CTCAGGCTCC CCCGCCACCT 
GGAAATGGGA GGTCACAGCC AACTTCAGGG TGGAAGGGTG GTCCCCAGCC AACTGTTTCC 
AAGGAGGAAG AGCCAAGTGG CTGGGAGGAA CCATCCTCAC AATCCATTGG TCATAAAATG 
GACATTGATG ACGGAACAGC AGCTTGGGGG GATCCTAACA ATTACAATTA CAAAAATGTC 
AGCCTGTGGG ACAAGAATGC TCAAGCAGGC CCTGGGCAGA TGGCTCGGGA GCAAGGAATA 
TCTGGGACAT TGGCAAACAA GGCAGCTCCC TCAGTGTGGA GTAAAAACAA AGGACCCCCA 
ACTGACAATG GGACATCAGC TTGGGGTGAG CCAACTGAAC CTGCTTCAGG GTGGGGAGAA 
GGTGAGGAGT CGGGTCCACC AACACCAGGA TGGGGAAGTG CACCCCAAGT AAATCCCAGT 
GCCATGAAAT CTGGTACAAA ATCTATGCAA GATGGCTGGG GTGAGAGTGA TGGGCCGGTG 
ACAGGAACAC GTCACAGCAG CTGGGATGAA GAAGAAGAGG GTGGAGTCTG GAACAGTGCA 
GGTTCTCAGG GAAGTGGCTC CTCCCACAAC TCTGCAGGTT GGGGACAAGG AGGAAAGAAA 
CCACAGATCA AGGGTCCCTT AAAAGGAGGG AGCAATGATA CCTGGATGAA CACCTTGCCC 
AAACAGTTCT CCAACATGGG ATTGTTGAGC CAAGTTGATG ATCCCTCTAC CACTAAGATG 
GACCATCCTG GCGGGCTTCC AGATAAGAAG TTTGAGACAG ACAAGCGGGG CGTGAATCTC 
GGGGATTTTA ATGACATGAT GCGGAAGGAA CGGTCTGGCT TCCGTCTGAA CAATTCCAAA 
GAGATGGGAG CTGCAGACAG TGGGCCTTAT TTTGAGAAGC TGACTTTGCC TTTCTCCAAT 
CAAGACGGGT GCCTTGGGGA TGAGGCTCCC TGTTCCCCTT TTTCTCCCTC CCCTAGCTAC 
AAGCTATCTC CCTCCGGCTC CTCGCTCCCT TCCGTGGGCC TGGGGGCTAT CGGTTCTGGC 
CTCAACCCCC AAACCTTCGC TGCTAGACAA GGTGGCAATC ACGGACTATT TGGAAACAGC 
GGTGCACAAT CGCGAGGCAT GCACAACCCT GTGCCATCGC TCAACTCCTC TCCCAACCTC 
CGGGCACAAG TGCCTCCCCA GTTCCTCTCT CCCCAGGTCT CAGCCTCCAT GCTAAAGCAG 
TTTCCTAACA GTAACATGAA TCCAGGACTT TTCAATATGG GGCCACAGCT TTCCCCTCAG 
CAGATTGCAA TGCTGAGCCA GCTTCCTCAA ATTCAGCAGT TCCAGCTGGC ATGTCAGCTC 
CTCTTGCAAC AACAACAACA GCAGCAACAG CAGCAGCAGC AGCAGCAACT CCTTCAGAAC 
CAGAGGAAGA TCTCACAAGC GGTCAGACAG CAGCAGGAAC AGCAGCAGAT AGCACGCATG 
GTAAGTGCCC TACAACAGCA GCAACAGCAA CAGCAGCAGC AGCAACAGAG GCAGCCAGGA 
ATGAAGCACT CTCCCTCTCA CCCTGCTGGA CCCAAAGGTC ACTTAGATGC TGTTGGACTC 
TCAGACATGC AAGCCAAAGG GCACATGCCA TATGGATCTG GTTTTGGCTC AAGTGGGATG 
GATTATGGTA CAGTAGGTAA GGAGGCGGGA GCAGAGTCTC GATTTAAACA GTGGACATCC 
ATGATGGAGG GGCTACCGTC CACTGTTTCT CAAGATACCA GCCTGCATAA AAATGGCTCC 
ATTGCACCCC CAGTAAAAAA TCGTGGAGGT TCCCCCTACC ACCAATATGA CATGTTGTCT 
ACGGATGCTT TGGGAGGGCA TGGGGGGCCA ACAGCTGAGA GCTGGTTACC TGCCAAATCA 
CCCCCTTCAA GCAAAATTGG AAACAAGTCA ATCAATGCCA GCTGGCCCCC AGAGTTTCAG 
CCAGGAGTGC CATGGAAAGG GATCCAAAAT ATTGATCCAG AATCTGATCC TTATGTTACC 
CCTGGCAGTG TGCTTGGGGG TCCAGCCCCA TCTCCCATTG TAGATACTGA CCACCAGCTA 
CTGCAGGACA CCCCCACAGG GTCCAATTCT TCCCTCAACA CCTCGCTGCC TTCACCTGGT 
GCCTGGCCCT TTAGTGCCTC TGAGAATATC CATAGCACTT CAGCACTCTC TGCAAAGTTT 
GGGGACTACA AATCGACCTG GTCACCAGAC CCCATAGGAC ATAACCCATC ACATCACTCA 
AACAAGATGT GGAAAAACCA GATGTCCAGG AACAGCCCTG GGCTGCCTCG CCCACCTCCA 
GGGCTCACAA ACAACCCCAA AGCCACCAAC TCTCCCTGGA ACAGCACAGC ACCTCGCTCC 
GTCAGGGGCT GGGGCGGACA GGACTCCAGA ATGGCTTCTG CATCAGCTTG GAGTGATGGT 
GGATCTGTGC GCCCCAGCTA CTGGCTGATC CTTCTTAATC TCACACCACA GATCGATGGT 
TCCACCCTGC GAACGATCTG CATGCAGCAT GGCCCCCTGC TGACATTCCA TCTCAACCTT 
ACCCAGGGCA CAGCTCTAAT CCGATACAGC ACCAAACAGG AGGCAGCCAA GGCCCAGAGT 
GCCCTCCATA TGTGTGTTCT GGGTAATACA ACTATCCTGG CGGAGTTTGC AACGGATGAG 
GAGGTGAGCC GGTATCTTGC ACAAGCTCAG CCACCAGCTC CCTCTGCTCC AGGAGCAGGC 
TGGCAGCCTT TGGAAGTGGG ACCGAACCAC CCAGCTGAAC CAGTAGGGCC AGCCCTAAAC 
CTTTTTGGAG GAACCACAGG GCTAGGGCAA TGGAGCAGCG GCGGTGGTGG TAGTGACCTG 
TCAGGGGTTT CTCTCTGGGG TACTCCTAGC AGCTACACAT CCAGTCTGTG GGGTGTACCG 
AGCACAGAGG ACGCCCACAG GATGGGCAGC CCTGCCCCGT TACTACCCGG TGACCTTCTG 
GGAGGAGGGA CCGACTCGAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816932.2
CDS	387..5990
Translation	MREKHLKKAVPREKEQEREEQLMEDKKRKKEDKKKKEAAQKVTEQKTKVPEVTKPSSSQATAASLPGSSPSPPVNGGNNAKRVAVPNGQPPSTARYMPREVPPRFRCQQDHKVLLKRGQPPPASCMLPGGGAGTPPSTTAPGAGPGNAQPGAGTLLPGESGASTDSSSGAAATSNYANSTWGSGGPTSSGPTTNPPHAWDKVIVDGSDMEEWPCIAGVDAEPPSDNATDNNSASNSASEKGPPQGSTTSHKGRGGQGQQQQQQQPGSECIQGVWKSDPKAKPVPSSNPASEGVNRLGNWRNGGGSERAGSASGFSNFNPNTNPSAWPALVQEGNRKGNPESDSSFSGAQLGPVGLTSREQQSKMENAGSSFAGSGRESATTHHTDGPKNGNTNSTNSNTLNPPDNKGTAFGVGSMDSCRGSDSPAQSTGERKNGGIGSWGAGRGQSATGDANCGHSNSGSNGREREDARKAPSSHRPNGSRGESWESKSGNGSWGYSPQADLSEVKWSEGNKLGAGGASQGEWKQPAGHEDLKVGEWGGLNQGNSSTGAWDNQKGHSLSDNQGNTSSAACWGRAPSSAGSEAGGQSTGSNAKAGGSGGSGDSLNSSSRRSHRPALPDSQAALQTLLNRTDLDPRVLSNSGWGQTQIKQDASWESEEVPSRTEGKSDRGTEGWENVSQAKTSGGWGDAPSQSNQNKSGWGEPPVPAPSTQEWKDTKATGWNDYKNSPTGWVGGGGGGVVGARPEDKAVPGWNNESGGAAEPGWSTRQSNPGGGWSAGKNGWGGNGSEDMEPPKAPGGWEGSGSKSSSGWGEVGQNDVGSWGNGANSNPAPRTGWEETKRPQGGGNWGEAARPQLPWNKQQESTPSSATPSTWGGSQAPPPPGNGRSQPTSGWKGGPQPTVSKEEEPSGWEEPSSQSIGHKMDIDDGTAAWGDPNNYNYKNVSLWDKNAQAGPGQMAREQGISGTLANKAAPSVWSKNKGPPTDNGTSAWGEPTEPASGWGEGEESGPPTPGWGSAPQVNPSAMKSGTKSMQDGWGESDGPVTGTRHSSWDEEEEGGVWNSAGSQGSGSSHNSAGWGQGGKKPQIKGPLKGGSNDTWMNTLPKQFSNMGLLSQVDDPSTTKMDHPGGLPDKKFETDKRGVNLGDFNDMMRKERSGFRLNNSKEMGAADSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSSLPSVGLGAIGSGLNPQTFAARQGGNHGLFGNSGAQSRGMHNPVPSLNSSPNLRAQVPPQFLSPQVSASMLKQFPNSNMNPGLFNMGPQLSPQQIAMLSQLPQIQQFQLACQLLLQQQQQQQQQQQQQQLLQNQRKISQAVRQQQEQQQIARMVSALQQQQQQQQQQQQRQPGMKHSPSHPAGPKGHLDAVGLSDMQAKGHMPYGSGFGSSGMDYGTVGKEAGAESRFKQWTSMMEGLPSTVSQDTSLHKNGSIAPPVKNRGGSPYHQYDMLSTDALGGHGGPTAESWLPAKSPPSSKIGNKSINASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGPAPSPIVDTDHQLLQDTPTGSNSSLNTSLPSPGAWPFSASENIHSTSALSAKFGDYKSTWSPDPIGHNPSHHSNKMWKNQMSRNSPGLPRPPPGLTNNPKATNSPWNSTAPRSVRGWGGQDSRMASASAWSDGGSVRPSYWLILLNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQSALHMCVLGNTTILAEFATDEEVSRYLAQAQPPAPSAPGAGWQPLEVGPNHPAEPVGPALNLFGGTTGLGQWSSGGGGSDLSGVSLWGTPSSYTSSLWGVPSTEDAHRMGSPAPLLPGDLLGGGTDSI

Target ORF information:

RefSeq Version	XM_012961478.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961478.3

ORF Insert Sequence:

ATGAGGGAGA AACACTTGAA GAAAGCTGTT CCCAGAGAGA AGGAGCAAGA AAGGGAAGAA 
CAGTTAATGG AAGACAAGAA AAGGAAGAAA GAGGATAAAA AGAAGAAAGA AGCTGCTCAG 
AAGGTCACAG AACAAAAAAC CAAAGTGCCC GAAGTGACGA AACCAAGTTC AAGCCAAGCG 
ACGGCCGCCA GCCTACCTGG CAGCTCCCCC TCGCCACCAG TCAATGGTGG CAACAATGCC 
AAAAGGGTGG CAGTGCCGAA CGGACAACCG CCCAGCACCG CCCGCTATAT GCCTCGGGAG 
GTGCCGCCGC GATTCCGCTG CCAGCAGGAC CACAAAGTGT TACTGAAGCG TGGACAGCCT 
CCTCCAGCGT CCTGTATGCT GCCGGGGGGA GGAGCAGGGA CTCCCCCCTC AACCACTGCT 
CCAGGGGCCG GCCCAGGCAA CGCACAGCCA GGGGCGGGGA CACTGCTGCC AGGGGAAAGC 
GGGGCTTCAA CAGACTCATC ATCGGGGGCA GCTGCTACTT CAAATTATGC AAATTCCACT 
TGGGGCTCAG GAGGCCCCAC CAGCAGCGGC CCCACCACAA ACCCTCCCCA CGCCTGGGAC 
AAAGTGATTG TAGACGGGTC GGACATGGAA GAGTGGCCTT GCATAGCCGG CGTGGACGCA 
GAGCCCCCTT CCGACAACGC CACAGACAAC AACAGTGCCT CGAACTCAGC CTCGGAGAAG 
GGACCCCCAC AAGGAAGCAC CACTAGCCAC AAAGGGCGAG GGGGCCAGGG CCAGCAGCAA 
CAGCAGCAGC AGCCTGGTAG TGAATGTATC CAGGGCGTCT GGAAATCTGA CCCCAAGGCT 
AAGCCCGTCC CGTCTTCCAA CCCTGCTTCA GAGGGTGTAA ATAGACTGGG AAACTGGAGA 
AATGGGGGTG GGTCAGAGAG AGCAGGTTCT GCCTCTGGTT TCAGCAACTT TAATCCAAAT 
ACTAACCCAT CTGCTTGGCC TGCCCTTGTC CAGGAAGGCA ACAGGAAAGG GAACCCAGAG 
TCAGACAGCA GCTTTTCTGG CGCACAACTC GGCCCAGTGG GTTTGACCTC TCGGGAGCAG 
CAGTCAAAGA TGGAAAATGC AGGTTCCAGC TTTGCAGGAT CGGGCAGGGA GTCTGCCACA 
ACCCATCACA CTGACGGACC AAAAAATGGA AACACTAACT CTACGAACTC AAATACTTTG 
AACCCTCCTG ATAACAAGGG AACAGCTTTT GGGGTGGGCT CAATGGATTC CTGCCGGGGT 
TCAGACTCCC CTGCTCAAAG CACTGGAGAA AGAAAGAATG GGGGGATTGG CTCTTGGGGA 
GCGGGAAGGG GTCAGTCTGC CACAGGAGAT GCTAATTGTG GACATAGCAA CTCTGGGAGC 
AATGGACGAG AGAGAGAAGA CGCAAGGAAA GCGCCTTCAT CTCATAGACC CAATGGGTCA 
AGGGGGGAAT CTTGGGAAAG CAAAAGTGGG AATGGTTCTT GGGGTTACAG CCCGCAGGCA 
GACCTCAGTG AAGTGAAATG GAGTGAAGGG AACAAATTGG GGGCTGGAGG GGCGTCTCAG 
GGAGAATGGA AGCAGCCAGC CGGGCATGAG GACTTAAAGG TGGGAGAGTG GGGAGGCTTG 
AACCAAGGAA ACTCTAGCAC TGGAGCATGG GACAATCAGA AGGGCCACTC TCTATCGGAT 
AACCAGGGCA ACACGTCATC AGCAGCCTGC TGGGGCCGAG CCCCCAGCTC TGCAGGAAGT 
GAAGCTGGGG GGCAAAGCAC TGGAAGCAAC GCTAAAGCAG GGGGGAGCGG AGGAAGCGGA 
GACAGCCTGA ACTCCAGCAG TCGACGTTCA CACAGACCTG CCCTCCCAGA CAGTCAGGCT 
GCTCTTCAGA CTTTACTGAA TCGCACTGAT TTGGATCCCC GGGTGCTGTC CAACTCTGGC 
TGGGGCCAAA CCCAGATCAA GCAAGATGCA TCATGGGAGA GTGAGGAAGT GCCCTCTCGA 
ACTGAAGGCA AATCAGACCG TGGGACTGAA GGTTGGGAGA ACGTGTCCCA AGCCAAAACC 
TCAGGGGGTT GGGGGGATGC TCCCAGCCAA AGCAATCAAA ACAAGTCAGG CTGGGGGGAG 
CCCCCTGTCC CTGCACCTTC AACCCAAGAA TGGAAGGACA CCAAAGCAAC TGGCTGGAAT 
GACTATAAAA ACAGCCCAAC TGGTTGGGTT GGTGGAGGAG GTGGTGGGGT GGTTGGAGCA 
CGACCAGAGG ACAAGGCAGT TCCAGGTTGG AACAATGAAA GTGGTGGGGC TGCGGAGCCT 
GGGTGGAGTA CTCGCCAGTC TAATCCAGGA GGTGGCTGGT CAGCAGGAAA GAATGGTTGG 
GGGGGAAATG GTAGTGAGGA CATGGAGCCT CCCAAGGCCC CTGGAGGCTG GGAAGGCTCA 
GGAAGCAAAT CCTCCTCTGG CTGGGGTGAA GTTGGACAAA ATGATGTGGG TTCTTGGGGT 
AATGGGGCAA ACTCAAATCC AGCTCCACGA ACTGGCTGGG AGGAGACTAA GAGGCCCCAG 
GGAGGAGGAA ACTGGGGTGA GGCAGCTAGG CCCCAACTAC CCTGGAACAA ACAGCAGGAA 
TCAACGCCAT CTTCAGCAAC TCCTAGCACC TGGGGGGGCT CTCAGGCTCC CCCGCCACCT 
GGAAATGGGA GGTCACAGCC AACTTCAGGG TGGAAGGGTG GTCCCCAGCC AACTGTTTCC 
AAGGAGGAAG AGCCAAGTGG CTGGGAGGAA CCATCCTCAC AATCCATTGG TCATAAAATG 
GACATTGATG ACGGAACAGC AGCTTGGGGG GATCCTAACA ATTACAATTA CAAAAATGTC 
AGCCTGTGGG ACAAGAATGC TCAAGCAGGC CCTGGGCAGA TGGCTCGGGA GCAAGGAATA 
TCTGGGACAT TGGCAAACAA GGCAGCTCCC TCAGTGTGGA GTAAAAACAA AGGACCCCCA 
ACTGACAATG GGACATCAGC TTGGGGTGAG CCAACTGAAC CTGCTTCAGG GTGGGGAGAA 
GGTGAGGAGT CGGGTCCACC AACACCAGGA TGGGGAAGTG CACCCCAAGT AAATCCCAGT 
GCCATGAAAT CTGGTACAAA ATCTATGCAA GATGGCTGGG GTGAGAGTGA TGGGCCGGTG 
ACAGGAACAC GTCACAGCAG CTGGGATGAA GAAGAAGAGG GTGGAGTCTG GAACAGTGCA 
GGTTCTCAGG GAAGTGGCTC CTCCCACAAC TCTGCAGGTT GGGGACAAGG AGGAAAGAAA 
CCACAGATCA AGGGTCCCTT AAAAGGAGGG AGCAATGATA CCTGGATGAA CACCTTGCCC 
AAACAGTTCT CCAACATGGG ATTGTTGAGC CAAGTTGATG ATCCCTCTAC CACTAAGATG 
GACCATCCTG GCGGGCTTCC AGATAAGAAG TTTGAGACAG ACAAGCGGGG CGTGAATCTC 
GGGGATTTTA ATGACATGAT GCGGAAGGAA CGGTCTGGCT TCCGTCTGAA CAATTCCAAA 
GAGATGGGAG CTGCAGACAG TGGGCCTTAT TTTGAGAAGC TGACTTTGCC TTTCTCCAAT 
CAAGACGGGT GCCTTGGGGA TGAGGCTCCC TGTTCCCCTT TTTCTCCCTC CCCTAGCTAC 
AAGCTATCTC CCTCCGGCTC CTCGCTCCCT TCCGTGGGCC TGGGGGCTAT CGGTTCTGGC 
CTCAACCCCC AAACCTTCGC TGCTAGACAA GGTGGCAATC ACGGACTATT TGGAAACAGC 
GGTGCACAAT CGCGAGGCAT GCACAACCCT GTGCCATCGC TCAACTCCTC TCCCAACCTC 
CGGGCACAAG TGCCTCCCCA GTTCCTCTCT CCCCAGGTCT CAGCCTCCAT GCTAAAGCAG 
TTTCCTAACA GTAACATGAA TCCAGGACTT TTCAATATGG GGCCACAGCT TTCCCCTCAG 
CAGATTGCAA TGCTGAGCCA GCTTCCTCAA ATTCAGCAGT TCCAGCTGGC ATGTCAGCTC 
CTCTTGCAAC AACAACAACA GCAGCAACAG CAGCAGCAGC AGCAGCAACT CCTTCAGAAC 
CAGAGGAAGA TCTCACAAGC GGTCAGACAG CAGCAGGAAC AGCAGCAGAT AGCACGCATG 
GTAAGTGCCC TACAACAGCA GCAACAGCAA CAGCAGCAGC AGCAACAGAG GCAGCCAGGA 
ATGAAGCACT CTCCCTCTCA CCCTGCTGGA CCCAAAGGTC ACTTAGATGC TGTTGGACTC 
TCAGACATGC AAGCCAAAGG GCACATGCCA TATGGATCTG GTTTTGGCTC AAGTGGGATG 
GATTATGGTA CAGTAGGTAA GGAGGCGGGA GCAGAGTCTC GATTTAAACA GTGGACATCC 
ATGATGGAGG GGCTACCGTC CACTGTTTCT CAAGATACCA GCCTGCATAA AAATGGCTCC 
ATTGCACCCC CAGTAAAAAA TCGTGGAGGT TCCCCCTACC ACCAATATGA CATGTTGTCT 
ACGGATGCTT TGGGAGGGCA TGGGGGGCCA ACAGCTGAGA GCTGGTTACC TGCCAAATCA 
CCCCCTTCAA GCAAAATTGG AAACAAGTCA ATCAATGCCA GCTGGCCCCC AGAGTTTCAG 
CCAGGAGTGC CATGGAAAGG GATCCAAAAT ATTGATCCAG AATCTGATCC TTATGTTACC 
CCTGGCAGTG TGCTTGGGGG TCCAGCCCCA TCTCCCATTG TAGATACTGA CCACCAGCTA 
CTGCAGGACA CCCCCACAGG GTCCAATTCT TCCCTCAACA CCTCGCTGCC TTCACCTGGT 
GCCTGGCCCT TTAGTGCCTC TGAGAATATC CATAGCACTT CAGCACTCTC TGCAAAGTTT 
GGGGACTACA AATCGACCTG GTCACCAGAC CCCATAGGAC ATAACCCATC ACATCACTCA 
AACAAGATGT GGAAAAACCA GATGTCCAGG AACAGCCCTG GGCTGCCTCG CCCACCTCCA 
GGGCTCACAA ACAACCCCAA AGCCACCAAC TCTCCCTGGA ACAGCACAGC ACCTCGCTCC 
GTCAGGGGCT GGGGCGGACA GGACTCCAGA ATGGCTTCTG CATCAGCTTG GAGTGATGGT 
GGATCTGTGC GCCCCAGCTA CTGGCTGATC CTTCTTAATC TCACACCACA GATCGATGGT 
TCCACCCTGC GAACGATCTG CATGCAGCAT GGCCCCCTGC TGACATTCCA TCTCAACCTT 
ACCCAGGGCA CAGCTCTAAT CCGATACAGC ACCAAACAGG AGGCAGCCAA GGCCCAGAGT 
GCCCTCCATA TGTGTGTTCT GGGTAATACA ACTATCCTGG CGGAGTTTGC AACGGATGAG 
GAGGTGAGCC GGTATCTTGC ACAAGCTCAG CCACCAGCTC CCTCTGCTCC AGGAGCAGGC 
TGGCAGCCTT TGGAAGTGGG ACCGAACCAC CCAGCTGAAC CAGTAGGGCC AGCCCTAAAC 
CTTTTTGGAG GAACCACAGG GCTAGGGCAA TGGAGCAGCG GCGGTGGTGG TAGTGACCTG 
TCAGGGGTTT CTCTCTGGGG TACTCCTAGC AGCTACACAT CCAGTCTGTG GGGTGTACCG 
AGCACAGAGG ACGCCCACAG GATGGGCAGC CCTGCCCCGT TACTACCCGG TGACCTTCTG 
GGAGGAGGGA CCGACTCGAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnrc6b ( XM_031901083.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756943.1 Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X7, mRNA.

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CloneID	OXa57436
Clone ID Related Accession (Same CDS sequence)	XM_031901083.1
Accession Version	XM_031901083.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trinucleotide repeat-containing gene 6B protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAATG GCTGCCAGTC TCTCGTTTAT GCACATTTCC CTTTAAAGAG GAGAAAACAG 
AAATATATAA ACAAGTGTGT TTGGAATTTA TTATTGTGTA TTGCATTTTT GCCTATTTTT 
ATTTTGTGTA CTTGCGTTCA TTTTTTTTGT ACTGGATTTA CCTTGTATGC CTCAAACCGC 
TGGATTATAG CACAGTTGCA CTTTATAAGA GAGAAGGAGC AAGAAAGGGA AGAACAGTTA 
ATGGAAGACA AGAAAAGGAA GAAAGAGGAT AAAAAGAAGA AAGAAGCTGC TCAGAAGGTC 
ACAGAACAAA AAACCAAAGT GCCCGAAGTG ACGAAACCAA GTTCAAGCCA AGCGACGGCC 
GCCAGCCTAC CTGGCAGCTC CCCCTCGCCA CCAGTCAATG GTGGCAACAA TGCCAAAAGG 
GTGGCAGTGC CGAACGGACA ACCGCCCAGC ACCGCCCGCT ATATGCCTCG GGAGGTGCCG 
CCGCGATTCC GCTGCCAGCA GGACCACAAA GTGTTACTGA AGCGTGGACA GCCTCCTCCA 
GCGTCCTGTA TGCTGCCGGG GGGAGGAGCA GGGACTCCCC CCTCAACCAC TGCTCCAGGG 
GCCGGCCCAG GCAACGCACA GCCAGGGGCG GGGACACTGC TGCCAGGGGA AAGCGGGGCT 
TCAACAGACT CATCATCGGG GGCAGCTGCT ACTTCAAATT ATGCAAATTC CACTTGGGGC 
TCAGGAGGCC CCACCAGCAG CGGCCCCACC ACAAACCCTC CCCACGCCTG GGACAAAGTG 
ATTGTAGACG GGTCGGACAT GGAAGAGTGG CCTTGCATAG CCGGCGTGGA CGCAGAGCCC 
CCTTCCGACA ACGCCACAGA CAACAACAGT GCCTCGAACT CAGCCTCGGA GAAGGGACCC 
CCACAAGGAA GCACCACTAG CCACAAAGGG CGAGGGGGCC AGGGCCAGCA GCAACAGCAG 
CAGCAGCCTG GTAGTGAATG TATCCAGGGC GTCTGGAAAT CTGACCCCAA GGCTAAGCCC 
GTCCCGTCTT CCAACCCTGC TTCAGAGGGT GTAAATAGAC TGGGAAACTG GAGAAATGGG 
GGTGGGTCAG AGAGAGCAGG TTCTGCCTCT GGTTTCAGCA ACTTTAATCC AAATACTAAC 
CCATCTGCTT GGCCTGCCCT TGTCCAGGAA GGCAACAGGA AAGGGAACCC AGAGTCAGAC 
AGCAGCTTTT CTGGCGCACA ACTCGGCCCA GTGGGTTTGA CCTCTCGGGA GCAGCAGTCA 
AAGATGGAAA ATGCAGGTTC CAGCTTTGCA GGATCGGGCA GGGAGTCTGC CACAACCCAT 
CACACTGACG GACCAAAAAA TGGAAACACT AACTCTACGA ACTCAAATAC TTTGAACCCT 
CCTGATAACA AGGGAACAGC TTTTGGGGTG GGCTCAATGG ATTCCTGCCG GGGTTCAGAC 
TCCCCTGCTC AAAGCACTGG AGAAAGAAAG AATGGGGGGA TTGGCTCTTG GGGAGCGGGA 
AGGGGTCAGT CTGCCACAGG AGATGCTAAT TGTGGACATA GCAACTCTGG GAGCAATGGA 
CGAGAGAGAG AAGACGCAAG GAAAGCGCCT TCATCTCATA GACCCAATGG GTCAAGGGGG 
GAATCTTGGG AAAGCAAAAG TGGGAATGGT TCTTGGGGTT ACAGCCCGCA GGCAGACCTC 
AGTGAAGTGA AATGGAGTGA AGGGAACAAA TTGGGGGCTG GAGGGGCGTC TCAGGGAGAA 
TGGAAGCAGC CAGCCGGGCA TGAGGACTTA AAGGTGGGAG AGTGGGGAGG CTTGAACCAA 
GGAAACTCTA GCACTGGAGC ATGGGACAAT CAGAAGGGCC ACTCTCTATC GGATAACCAG 
GGCAACACGT CATCAGCAGC CTGCTGGGGC CGAGCCCCCA GCTCTGCAGG AAGTGAAGCT 
GGGGGGCAAA GCACTGGAAG CAACGCTAAA GCAGGGGGGA GCGGAGGAAG CGGAGACAGC 
CTGAACTCCA GCAGTCGACG TTCACACAGA CCTGCCCTCC CAGACAGTCA GGCTGCTCTT 
CAGACTTTAC TGAATCGCAC TGATTTGGAT CCCCGGGTGC TGTCCAACTC TGGCTGGGGC 
CAAACCCAGA TCAAGCAAGA TGCATCATGG GAGAGTGAGG AAGTGCCCTC TCGAACTGAA 
GGCAAATCAG ACCGTGGGAC TGAAGGTTGG GAGAACGTGT CCCAAGCCAA AACCTCAGGG 
GGTTGGGGGG ATGCTCCCAG CCAAAGCAAT CAAAACAAGT CAGGCTGGGG GGAGCCCCCT 
GTCCCTGCAC CTTCAACCCA AGAATGGAAG GACACCAAAG CAACTGGCTG GAATGACTAT 
AAAAACAGCC CAACTGGTTG GGTTGGTGGA GGAGGTGGTG GGGTGGTTGG AGCACGACCA 
GAGGACAAGG CAGTTCCAGG TTGGAACAAT GAAAGTGGTG GGGCTGCGGA GCCTGGGTGG 
AGTACTCGCC AGTCTAATCC AGGAGGTGGC TGGTCAGCAG GAAAGAATGG TTGGGGGGGA 
AATGGTAGTG AGGACATGGA GCCTCCCAAG GCCCCTGGAG GCTGGGAAGG CTCAGGAAGC 
AAATCCTCCT CTGGCTGGGG TGAAGTTGGA CAAAATGATG TGGGTTCTTG GGGTAATGGG 
GCAAACTCAA ATCCAGCTCC ACGAACTGGC TGGGAGGAGA CTAAGAGGCC CCAGGGAGGA 
GGAAACTGGG GTGAGGCAGC TAGGCCCCAA CTACCCTGGA ACAAACAGCA GGAATCAACG 
CCATCTTCAG CAACTCCTAG CACCTGGGGG GGCTCTCAGG CTCCCCCGCC ACCTGGAAAT 
GGGAGGTCAC AGCCAACTTC AGGGTGGAAG GGTGGTCCCC AGCCAACTGT TTCCAAGGAG 
GAAGAGCCAA GTGGCTGGGA GGAACCATCC TCACAATCCA TTGGTCATAA AATGGACATT 
GATGACGGAA CAGCAGCTTG GGGGGATCCT AACAATTACA ATTACAAAAA TGTCAGCCTG 
TGGGACAAGA ATGCTCAAGC AGGCCCTGGG CAGATGGCTC GGGAGCAAGG AATATCTGGG 
ACATTGGCAA ACAAGGCAGC TCCCTCAGTG TGGAGTAAAA ACAAAGGACC CCCAACTGAC 
AATGGGACAT CAGCTTGGGG TGAGCCAACT GAACCTGCTT CAGGGTGGGG AGAAGGTGAG 
GAGTCGGGTC CACCAACACC AGGATGGGGA AGTGCACCCC AAGTAAATCC CAGTGCCATG 
AAATCTGGTA CAAAATCTAT GCAAGATGGC TGGGGTGAGA GTGATGGGCC GGTGACAGGA 
ACACGTCACA GCAGCTGGGA TGAAGAAGAA GAGGGTGGAG TCTGGAACAG TGCAGGTTCT 
CAGGGAAGTG GCTCCTCCCA CAACTCTGCA GGTTGGGGAC AAGGAGGAAA GAAACCACAG 
ATCAAGGGTC CCTTAAAAGG AGGGAGCAAT GATACCTGGA TGAACACCTT GCCCAAACAG 
TTCTCCAACA TGGGATTGTT GAGCCAAGTT GATGATCCCT CTACCACTAA GATGGACCAT 
CCTGGCGGGC TTCCAGATAA GAAGTTTGAG ACAGACAAGC GGGGCGTGAA TCTCGGGGAT 
TTTAATGACA TGATGCGGAA GGAACGGTCT GGCTTCCGTC TGAACAATTC CAAAGAGATG 
GGAGCTGCAG ACAGTGGGCC TTATTTTGAG AAGGGTGGCA ATCACGGACT ATTTGGAAAC 
AGCGGTGCAC AATCGCGAGG CATGCACAAC CCTGTGCCAT CGCTCAACTC CTCTCCCAAC 
CTCCGGGCAC AAGTGCCTCC CCAGTTCCTC TCTCCCCAGG TCTCAGCCTC CATGCTAAAG 
CAGTTTCCTA ACAGTAACAT GAATCCAGGA CTTTTCAATA TGGGGCCACA GCTTTCCCCT 
CAGCAGATTG CAATGCTGAG CCAGCTTCCT CAAATTCAGC AGTTCCAGCT GGCATGTCAG 
CTCCTCTTGC AACAACAACA ACAGCAGCAA CAGCAGCAGC AGCAGCAGCA ACTCCTTCAG 
AACCAGAGGA AGATCTCACA AGCGGTCAGA CAGCAGCAGG AACAGCAGAT AGCACGCATG 
GTAAGTGCCC TACAACAGCA GCAACAGCAA CAGCAGCAGC AGCAACAGAG GCAGCCAGGA 
ATGAAGCACT CTCCCTCTCA CCCTGCTGGA CCCAAAGGTC ACTTAGATGC TGTTGGACTC 
TCAGACATGC AAGCCAAAGG GCACATGCCA TATGGATCTG GTTTTGGCTC AAGTGGGATG 
GATTATGGTA CAGTAGGTAA GGAGGCGGGA GCAGAGTCTC GATTTAAACA GTGGACATCC 
ATGATGGAGG GGCTACCGTC CACTGTTTCT CAAGATACCA GCCTGCATAA AAATGGCTCC 
ATTGCACCCC CAGTAAAAAA TCGTGGAGGT TCCCCCTACC ACCAATATGA CATGTTGTCT 
ACGGATGCTT TGGGAGGGCA TGGGGGGCCA ACAGCTGAGA GCTGGTTACC TGCCAAATCA 
CCCCCTTCAA GCAAAATTGG AAACAAGTCA ATCAATGCCA GCTGGCCCCC AGAGTTTCAG 
CCAGGAGTGC CATGGAAAGG GATCCAAAAT ATTGATCCAG AATCTGATCC TTATGTTACC 
CCTGGCAGTG TGCTTGGGGG TCCAGCCCCA TCTCCCATTG TAGATACTGA CCACCAGCTA 
CTGCAGGACA CCCCCACAGG GTCCAATTCT TCCCTCAACA CCTCGCTGCC TTCACCTGGT 
GCCTGGCCCT TTAGTGCCTC TGAGAATATC CATAGCACTT CAGCACTCTC TGCAAAGTTT 
GGGGACTACA AATCGACCTG GTCACCAGAC CCCATAGGAC ATAACCCATC ACATCACTCA 
AACAAGATGT GGAAAAACCA GATGTCCAGG AACAGCCCTG GGCTGCCTCG CCCACCTCCA 
GGGCTCACAA ACAACCCCAA AGCCACCAAC TCTCCCTGGA ACAGCACAGC ACCTCGCTCC 
GTCAGGGGCT GGGGCGGACA GGACTCCAGA ATGGCTTCTG CATCAGCTTG GAGTGATGGT 
GGATCTGTGC GCCCCAGCTA CTGGCTGATC CTTCTTAATC TCACACCACA GATCGATGGT 
TCCACCCTGC GAACGATCTG CATGCAGCAT GGCCCCCTGC TGACATTCCA TCTCAACCTT 
ACCCAGGGCA CAGCTCTAAT CCGATACAGC ACCAAACAGG AGGCAGCCAA GGCCCAGAGT 
GCCCTCCATA TGTGTGTTCT GGGTAATACA ACTATCCTGG CGGAGTTTGC AACGGATGAG 
GAGGTGAGCC GGTATCTTGC ACAAGCTCAG CCACCAGCTC CCTCTGCTCC AGGAGCAGGC 
TGGCAGCCTT TGGAAGTGGG ACCGAACCAC CCAGCTGAAC CAGTAGGGCC AGCCCTAAAC 
CTTTTTGGAG GAACCACAGG GCTAGGGCAA TGGAGCAGCG GCGGTGGTGG TAGTGACCTG 
TCAGGGGTTT CTCTCTGGGG TACTCCTAGC AGCTACACAT CCAGTCTGTG GGGTGTACCG 
AGCACAGAGG ACGCCCACAG GATGGGCAGC CCTGCCCCGT TACTACCCGG TGACCTTCTG 
GGAGGAGGGA CCGACTCGAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756943.1
CDS	53..5656
Translation	MQNGCQSLVYAHFPLKRRKQKYINKCVWNLLLCIAFLPIFILCTCVHFFCTGFTLYASNRWIIAQLHFIREKEQEREEQLMEDKKRKKEDKKKKEAAQKVTEQKTKVPEVTKPSSSQATAASLPGSSPSPPVNGGNNAKRVAVPNGQPPSTARYMPREVPPRFRCQQDHKVLLKRGQPPPASCMLPGGGAGTPPSTTAPGAGPGNAQPGAGTLLPGESGASTDSSSGAAATSNYANSTWGSGGPTSSGPTTNPPHAWDKVIVDGSDMEEWPCIAGVDAEPPSDNATDNNSASNSASEKGPPQGSTTSHKGRGGQGQQQQQQQPGSECIQGVWKSDPKAKPVPSSNPASEGVNRLGNWRNGGGSERAGSASGFSNFNPNTNPSAWPALVQEGNRKGNPESDSSFSGAQLGPVGLTSREQQSKMENAGSSFAGSGRESATTHHTDGPKNGNTNSTNSNTLNPPDNKGTAFGVGSMDSCRGSDSPAQSTGERKNGGIGSWGAGRGQSATGDANCGHSNSGSNGREREDARKAPSSHRPNGSRGESWESKSGNGSWGYSPQADLSEVKWSEGNKLGAGGASQGEWKQPAGHEDLKVGEWGGLNQGNSSTGAWDNQKGHSLSDNQGNTSSAACWGRAPSSAGSEAGGQSTGSNAKAGGSGGSGDSLNSSSRRSHRPALPDSQAALQTLLNRTDLDPRVLSNSGWGQTQIKQDASWESEEVPSRTEGKSDRGTEGWENVSQAKTSGGWGDAPSQSNQNKSGWGEPPVPAPSTQEWKDTKATGWNDYKNSPTGWVGGGGGGVVGARPEDKAVPGWNNESGGAAEPGWSTRQSNPGGGWSAGKNGWGGNGSEDMEPPKAPGGWEGSGSKSSSGWGEVGQNDVGSWGNGANSNPAPRTGWEETKRPQGGGNWGEAARPQLPWNKQQESTPSSATPSTWGGSQAPPPPGNGRSQPTSGWKGGPQPTVSKEEEPSGWEEPSSQSIGHKMDIDDGTAAWGDPNNYNYKNVSLWDKNAQAGPGQMAREQGISGTLANKAAPSVWSKNKGPPTDNGTSAWGEPTEPASGWGEGEESGPPTPGWGSAPQVNPSAMKSGTKSMQDGWGESDGPVTGTRHSSWDEEEEGGVWNSAGSQGSGSSHNSAGWGQGGKKPQIKGPLKGGSNDTWMNTLPKQFSNMGLLSQVDDPSTTKMDHPGGLPDKKFETDKRGVNLGDFNDMMRKERSGFRLNNSKEMGAADSGPYFEKGGNHGLFGNSGAQSRGMHNPVPSLNSSPNLRAQVPPQFLSPQVSASMLKQFPNSNMNPGLFNMGPQLSPQQIAMLSQLPQIQQFQLACQLLLQQQQQQQQQQQQQQLLQNQRKISQAVRQQQEQQIARMVSALQQQQQQQQQQQQRQPGMKHSPSHPAGPKGHLDAVGLSDMQAKGHMPYGSGFGSSGMDYGTVGKEAGAESRFKQWTSMMEGLPSTVSQDTSLHKNGSIAPPVKNRGGSPYHQYDMLSTDALGGHGGPTAESWLPAKSPPSSKIGNKSINASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGPAPSPIVDTDHQLLQDTPTGSNSSLNTSLPSPGAWPFSASENIHSTSALSAKFGDYKSTWSPDPIGHNPSHHSNKMWKNQMSRNSPGLPRPPPGLTNNPKATNSPWNSTAPRSVRGWGGQDSRMASASAWSDGGSVRPSYWLILLNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQSALHMCVLGNTTILAEFATDEEVSRYLAQAQPPAPSAPGAGWQPLEVGPNHPAEPVGPALNLFGGTTGLGQWSSGGGGSDLSGVSLWGTPSSYTSSLWGVPSTEDAHRMGSPAPLLPGDLLGGGTDSI

Target ORF information:

RefSeq Version	XM_031901083.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901083.1

ORF Insert Sequence:

ATGCAAAATG GCTGCCAGTC TCTCGTTTAT GCACATTTCC CTTTAAAGAG GAGAAAACAG 
AAATATATAA ACAAGTGTGT TTGGAATTTA TTATTGTGTA TTGCATTTTT GCCTATTTTT 
ATTTTGTGTA CTTGCGTTCA TTTTTTTTGT ACTGGATTTA CCTTGTATGC CTCAAACCGC 
TGGATTATAG CACAGTTGCA CTTTATAAGA GAGAAGGAGC AAGAAAGGGA AGAACAGTTA 
ATGGAAGACA AGAAAAGGAA GAAAGAGGAT AAAAAGAAGA AAGAAGCTGC TCAGAAGGTC 
ACAGAACAAA AAACCAAAGT GCCCGAAGTG ACGAAACCAA GTTCAAGCCA AGCGACGGCC 
GCCAGCCTAC CTGGCAGCTC CCCCTCGCCA CCAGTCAATG GTGGCAACAA TGCCAAAAGG 
GTGGCAGTGC CGAACGGACA ACCGCCCAGC ACCGCCCGCT ATATGCCTCG GGAGGTGCCG 
CCGCGATTCC GCTGCCAGCA GGACCACAAA GTGTTACTGA AGCGTGGACA GCCTCCTCCA 
GCGTCCTGTA TGCTGCCGGG GGGAGGAGCA GGGACTCCCC CCTCAACCAC TGCTCCAGGG 
GCCGGCCCAG GCAACGCACA GCCAGGGGCG GGGACACTGC TGCCAGGGGA AAGCGGGGCT 
TCAACAGACT CATCATCGGG GGCAGCTGCT ACTTCAAATT ATGCAAATTC CACTTGGGGC 
TCAGGAGGCC CCACCAGCAG CGGCCCCACC ACAAACCCTC CCCACGCCTG GGACAAAGTG 
ATTGTAGACG GGTCGGACAT GGAAGAGTGG CCTTGCATAG CCGGCGTGGA CGCAGAGCCC 
CCTTCCGACA ACGCCACAGA CAACAACAGT GCCTCGAACT CAGCCTCGGA GAAGGGACCC 
CCACAAGGAA GCACCACTAG CCACAAAGGG CGAGGGGGCC AGGGCCAGCA GCAACAGCAG 
CAGCAGCCTG GTAGTGAATG TATCCAGGGC GTCTGGAAAT CTGACCCCAA GGCTAAGCCC 
GTCCCGTCTT CCAACCCTGC TTCAGAGGGT GTAAATAGAC TGGGAAACTG GAGAAATGGG 
GGTGGGTCAG AGAGAGCAGG TTCTGCCTCT GGTTTCAGCA ACTTTAATCC AAATACTAAC 
CCATCTGCTT GGCCTGCCCT TGTCCAGGAA GGCAACAGGA AAGGGAACCC AGAGTCAGAC 
AGCAGCTTTT CTGGCGCACA ACTCGGCCCA GTGGGTTTGA CCTCTCGGGA GCAGCAGTCA 
AAGATGGAAA ATGCAGGTTC CAGCTTTGCA GGATCGGGCA GGGAGTCTGC CACAACCCAT 
CACACTGACG GACCAAAAAA TGGAAACACT AACTCTACGA ACTCAAATAC TTTGAACCCT 
CCTGATAACA AGGGAACAGC TTTTGGGGTG GGCTCAATGG ATTCCTGCCG GGGTTCAGAC 
TCCCCTGCTC AAAGCACTGG AGAAAGAAAG AATGGGGGGA TTGGCTCTTG GGGAGCGGGA 
AGGGGTCAGT CTGCCACAGG AGATGCTAAT TGTGGACATA GCAACTCTGG GAGCAATGGA 
CGAGAGAGAG AAGACGCAAG GAAAGCGCCT TCATCTCATA GACCCAATGG GTCAAGGGGG 
GAATCTTGGG AAAGCAAAAG TGGGAATGGT TCTTGGGGTT ACAGCCCGCA GGCAGACCTC 
AGTGAAGTGA AATGGAGTGA AGGGAACAAA TTGGGGGCTG GAGGGGCGTC TCAGGGAGAA 
TGGAAGCAGC CAGCCGGGCA TGAGGACTTA AAGGTGGGAG AGTGGGGAGG CTTGAACCAA 
GGAAACTCTA GCACTGGAGC ATGGGACAAT CAGAAGGGCC ACTCTCTATC GGATAACCAG 
GGCAACACGT CATCAGCAGC CTGCTGGGGC CGAGCCCCCA GCTCTGCAGG AAGTGAAGCT 
GGGGGGCAAA GCACTGGAAG CAACGCTAAA GCAGGGGGGA GCGGAGGAAG CGGAGACAGC 
CTGAACTCCA GCAGTCGACG TTCACACAGA CCTGCCCTCC CAGACAGTCA GGCTGCTCTT 
CAGACTTTAC TGAATCGCAC TGATTTGGAT CCCCGGGTGC TGTCCAACTC TGGCTGGGGC 
CAAACCCAGA TCAAGCAAGA TGCATCATGG GAGAGTGAGG AAGTGCCCTC TCGAACTGAA 
GGCAAATCAG ACCGTGGGAC TGAAGGTTGG GAGAACGTGT CCCAAGCCAA AACCTCAGGG 
GGTTGGGGGG ATGCTCCCAG CCAAAGCAAT CAAAACAAGT CAGGCTGGGG GGAGCCCCCT 
GTCCCTGCAC CTTCAACCCA AGAATGGAAG GACACCAAAG CAACTGGCTG GAATGACTAT 
AAAAACAGCC CAACTGGTTG GGTTGGTGGA GGAGGTGGTG GGGTGGTTGG AGCACGACCA 
GAGGACAAGG CAGTTCCAGG TTGGAACAAT GAAAGTGGTG GGGCTGCGGA GCCTGGGTGG 
AGTACTCGCC AGTCTAATCC AGGAGGTGGC TGGTCAGCAG GAAAGAATGG TTGGGGGGGA 
AATGGTAGTG AGGACATGGA GCCTCCCAAG GCCCCTGGAG GCTGGGAAGG CTCAGGAAGC 
AAATCCTCCT CTGGCTGGGG TGAAGTTGGA CAAAATGATG TGGGTTCTTG GGGTAATGGG 
GCAAACTCAA ATCCAGCTCC ACGAACTGGC TGGGAGGAGA CTAAGAGGCC CCAGGGAGGA 
GGAAACTGGG GTGAGGCAGC TAGGCCCCAA CTACCCTGGA ACAAACAGCA GGAATCAACG 
CCATCTTCAG CAACTCCTAG CACCTGGGGG GGCTCTCAGG CTCCCCCGCC ACCTGGAAAT 
GGGAGGTCAC AGCCAACTTC AGGGTGGAAG GGTGGTCCCC AGCCAACTGT TTCCAAGGAG 
GAAGAGCCAA GTGGCTGGGA GGAACCATCC TCACAATCCA TTGGTCATAA AATGGACATT 
GATGACGGAA CAGCAGCTTG GGGGGATCCT AACAATTACA ATTACAAAAA TGTCAGCCTG 
TGGGACAAGA ATGCTCAAGC AGGCCCTGGG CAGATGGCTC GGGAGCAAGG AATATCTGGG 
ACATTGGCAA ACAAGGCAGC TCCCTCAGTG TGGAGTAAAA ACAAAGGACC CCCAACTGAC 
AATGGGACAT CAGCTTGGGG TGAGCCAACT GAACCTGCTT CAGGGTGGGG AGAAGGTGAG 
GAGTCGGGTC CACCAACACC AGGATGGGGA AGTGCACCCC AAGTAAATCC CAGTGCCATG 
AAATCTGGTA CAAAATCTAT GCAAGATGGC TGGGGTGAGA GTGATGGGCC GGTGACAGGA 
ACACGTCACA GCAGCTGGGA TGAAGAAGAA GAGGGTGGAG TCTGGAACAG TGCAGGTTCT 
CAGGGAAGTG GCTCCTCCCA CAACTCTGCA GGTTGGGGAC AAGGAGGAAA GAAACCACAG 
ATCAAGGGTC CCTTAAAAGG AGGGAGCAAT GATACCTGGA TGAACACCTT GCCCAAACAG 
TTCTCCAACA TGGGATTGTT GAGCCAAGTT GATGATCCCT CTACCACTAA GATGGACCAT 
CCTGGCGGGC TTCCAGATAA GAAGTTTGAG ACAGACAAGC GGGGCGTGAA TCTCGGGGAT 
TTTAATGACA TGATGCGGAA GGAACGGTCT GGCTTCCGTC TGAACAATTC CAAAGAGATG 
GGAGCTGCAG ACAGTGGGCC TTATTTTGAG AAGGGTGGCA ATCACGGACT ATTTGGAAAC 
AGCGGTGCAC AATCGCGAGG CATGCACAAC CCTGTGCCAT CGCTCAACTC CTCTCCCAAC 
CTCCGGGCAC AAGTGCCTCC CCAGTTCCTC TCTCCCCAGG TCTCAGCCTC CATGCTAAAG 
CAGTTTCCTA ACAGTAACAT GAATCCAGGA CTTTTCAATA TGGGGCCACA GCTTTCCCCT 
CAGCAGATTG CAATGCTGAG CCAGCTTCCT CAAATTCAGC AGTTCCAGCT GGCATGTCAG 
CTCCTCTTGC AACAACAACA ACAGCAGCAA CAGCAGCAGC AGCAGCAGCA ACTCCTTCAG 
AACCAGAGGA AGATCTCACA AGCGGTCAGA CAGCAGCAGG AACAGCAGAT AGCACGCATG 
GTAAGTGCCC TACAACAGCA GCAACAGCAA CAGCAGCAGC AGCAACAGAG GCAGCCAGGA 
ATGAAGCACT CTCCCTCTCA CCCTGCTGGA CCCAAAGGTC ACTTAGATGC TGTTGGACTC 
TCAGACATGC AAGCCAAAGG GCACATGCCA TATGGATCTG GTTTTGGCTC AAGTGGGATG 
GATTATGGTA CAGTAGGTAA GGAGGCGGGA GCAGAGTCTC GATTTAAACA GTGGACATCC 
ATGATGGAGG GGCTACCGTC CACTGTTTCT CAAGATACCA GCCTGCATAA AAATGGCTCC 
ATTGCACCCC CAGTAAAAAA TCGTGGAGGT TCCCCCTACC ACCAATATGA CATGTTGTCT 
ACGGATGCTT TGGGAGGGCA TGGGGGGCCA ACAGCTGAGA GCTGGTTACC TGCCAAATCA 
CCCCCTTCAA GCAAAATTGG AAACAAGTCA ATCAATGCCA GCTGGCCCCC AGAGTTTCAG 
CCAGGAGTGC CATGGAAAGG GATCCAAAAT ATTGATCCAG AATCTGATCC TTATGTTACC 
CCTGGCAGTG TGCTTGGGGG TCCAGCCCCA TCTCCCATTG TAGATACTGA CCACCAGCTA 
CTGCAGGACA CCCCCACAGG GTCCAATTCT TCCCTCAACA CCTCGCTGCC TTCACCTGGT 
GCCTGGCCCT TTAGTGCCTC TGAGAATATC CATAGCACTT CAGCACTCTC TGCAAAGTTT 
GGGGACTACA AATCGACCTG GTCACCAGAC CCCATAGGAC ATAACCCATC ACATCACTCA 
AACAAGATGT GGAAAAACCA GATGTCCAGG AACAGCCCTG GGCTGCCTCG CCCACCTCCA 
GGGCTCACAA ACAACCCCAA AGCCACCAAC TCTCCCTGGA ACAGCACAGC ACCTCGCTCC 
GTCAGGGGCT GGGGCGGACA GGACTCCAGA ATGGCTTCTG CATCAGCTTG GAGTGATGGT 
GGATCTGTGC GCCCCAGCTA CTGGCTGATC CTTCTTAATC TCACACCACA GATCGATGGT 
TCCACCCTGC GAACGATCTG CATGCAGCAT GGCCCCCTGC TGACATTCCA TCTCAACCTT 
ACCCAGGGCA CAGCTCTAAT CCGATACAGC ACCAAACAGG AGGCAGCCAA GGCCCAGAGT 
GCCCTCCATA TGTGTGTTCT GGGTAATACA ACTATCCTGG CGGAGTTTGC AACGGATGAG 
GAGGTGAGCC GGTATCTTGC ACAAGCTCAG CCACCAGCTC CCTCTGCTCC AGGAGCAGGC 
TGGCAGCCTT TGGAAGTGGG ACCGAACCAC CCAGCTGAAC CAGTAGGGCC AGCCCTAAAC 
CTTTTTGGAG GAACCACAGG GCTAGGGCAA TGGAGCAGCG GCGGTGGTGG TAGTGACCTG 
TCAGGGGTTT CTCTCTGGGG TACTCCTAGC AGCTACACAT CCAGTCTGTG GGGTGTACCG 
AGCACAGAGG ACGCCCACAG GATGGGCAGC CCTGCCCCGT TACTACCCGG TGACCTTCTG 
GGAGGAGGGA CCGACTCGAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnrc6b ( XM_012961481.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816935.2 Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X8, mRNA.

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CloneID	OXa57437
Clone ID Related Accession (Same CDS sequence)	XM_012961481.3
Accession Version	XM_012961481.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trinucleotide repeat-containing gene 6B protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961481.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA AGAAAAGGAA GAAAGAGGAT AAAAAGAAGA AAGAAGCTGC TCAGAAGGTC 
ACAGAACAAA AAACCAAAGT GCCCGAAGTG ACGAAACCAA GTTCAAGCCA AGCGACGGCC 
GCCAGCCTAC CTGGCAGCTC CCCCTCGCCA CCAGTCAATG GTGGCAACAA TGCCAAAAGG 
GTGGCAGTGC CGAACGGACA ACCGCCCAGC ACCGCCCGCT ATATGCCTCG GGAGGTGCCG 
CCGCGATTCC GCTGCCAGCA GGACCACAAA GTGTTACTGA AGCGTGGACA GCCTCCTCCA 
GCGTCCTGTA TGCTGCCGGG GGGAGGAGCA GGGACTCCCC CCTCAACCAC TGCTCCAGGG 
GCCGGCCCAG GCAACGCACA GCCAGGGGCG GGGACACTGC TGCCAGGGGA AAGCGGGGCT 
TCAACAGACT CATCATCGGG GGCAGCTGCT ACTTCAAATT ATGCAAATTC CACTTGGGGC 
TCAGGAGGCC CCACCAGCAG CGGCCCCACC ACAAACCCTC CCCACGCCTG GGACAAAGTG 
ATTGTAGACG GGTCGGACAT GGAAGAGTGG CCTTGCATAG CCGGCGTGGA CGCAGAGCCC 
CCTTCCGACA ACGCCACAGA CAACAACAGT GCCTCGAACT CAGCCTCGGA GAAGGGACCC 
CCACAAGGAA GCACCACTAG CCACAAAGGG CGAGGGGGCC AGGGCCAGCA GCAACAGCAG 
CAGCAGCCTG GTAGTGAATG TATCCAGGGC GTCTGGAAAT CTGACCCCAA GGCTAAGCCC 
GTCCCGTCTT CCAACCCTGC TTCAGAGGGT GTAAATAGAC TGGGAAACTG GAGAAATGGG 
GGTGGGTCAG AGAGAGCAGG TTCTGCCTCT GGTTTCAGCA ACTTTAATCC AAATACTAAC 
CCATCTGCTT GGCCTGCCCT TGTCCAGGAA GGCAACAGGA AAGGGAACCC AGAGTCAGAC 
AGCAGCTTTT CTGGCGCACA ACTCGGCCCA GTGGGTTTGA CCTCTCGGGA GCAGCAGTCA 
AAGATGGAAA ATGCAGGTTC CAGCTTTGCA GGATCGGGCA GGGAGTCTGC CACAACCCAT 
CACACTGACG GACCAAAAAA TGGAAACACT AACTCTACGA ACTCAAATAC TTTGAACCCT 
CCTGATAACA AGGGAACAGC TTTTGGGGTG GGCTCAATGG ATTCCTGCCG GGGTTCAGAC 
TCCCCTGCTC AAAGCACTGG AGAAAGAAAG AATGGGGGGA TTGGCTCTTG GGGAGCGGGA 
AGGGGTCAGT CTGCCACAGG AGATGCTAAT TGTGGACATA GCAACTCTGG GAGCAATGGA 
CGAGAGAGAG AAGACGCAAG GAAAGCGCCT TCATCTCATA GACCCAATGG GTCAAGGGGG 
GAATCTTGGG AAAGCAAAAG TGGGAATGGT TCTTGGGGTT ACAGCCCGCA GGCAGACCTC 
AGTGAAGTGA AATGGAGTGA AGGGAACAAA TTGGGGGCTG GAGGGGCGTC TCAGGGAGAA 
TGGAAGCAGC CAGCCGGGCA TGAGGACTTA AAGGTGGGAG AGTGGGGAGG CTTGAACCAA 
GGAAACTCTA GCACTGGAGC ATGGGACAAT CAGAAGGGCC ACTCTCTATC GGATAACCAG 
GGCAACACGT CATCAGCAGC CTGCTGGGGC CGAGCCCCCA GCTCTGCAGG AAGTGAAGCT 
GGGGGGCAAA GCACTGGAAG CAACGCTAAA GCAGGGGGGA GCGGAGGAAG CGGAGACAGC 
CTGAACTCCA GCAGTCGACG TTCACACAGA CCTGCCCTCC CAGACAGTCA GGCTGCTCTT 
CAGACTTTAC TGAATCGCAC TGATTTGGAT CCCCGGGTGC TGTCCAACTC TGGCTGGGGC 
CAAACCCAGA TCAAGCAAGA TGCATCATGG GAGAGTGAGG AAGTGCCCTC TCGAACTGAA 
GGCAAATCAG ACCGTGGGAC TGAAGGTTGG GAGAACGTGT CCCAAGCCAA AACCTCAGGG 
GGTTGGGGGG ATGCTCCCAG CCAAAGCAAT CAAAACAAGT CAGGCTGGGG GGAGCCCCCT 
GTCCCTGCAC CTTCAACCCA AGAATGGAAG GACACCAAAG CAACTGGCTG GAATGACTAT 
AAAAACAGCC CAACTGGTTG GGTTGGTGGA GGAGGTGGTG GGGTGGTTGG AGCACGACCA 
GAGGACAAGG CAGTTCCAGG TTGGAACAAT GAAAGTGGTG GGGCTGCGGA GCCTGGGTGG 
AGTACTCGCC AGTCTAATCC AGGAGGTGGC TGGTCAGCAG GAAAGAATGG TTGGGGGGGA 
AATGGTAGTG AGGACATGGA GCCTCCCAAG GCCCCTGGAG GCTGGGAAGG CTCAGGAAGC 
AAATCCTCCT CTGGCTGGGG TGAAGTTGGA CAAAATGATG TGGGTTCTTG GGGTAATGGG 
GCAAACTCAA ATCCAGCTCC ACGAACTGGC TGGGAGGAGA CTAAGAGGCC CCAGGGAGGA 
GGAAACTGGG GTGAGGCAGC TAGGCCCCAA CTACCCTGGA ACAAACAGCA GGAATCAACG 
CCATCTTCAG CAACTCCTAG CACCTGGGGG GGCTCTCAGG CTCCCCCGCC ACCTGGAAAT 
GGGAGGTCAC AGCCAACTTC AGGGTGGAAG GGTGGTCCCC AGCCAACTGT TTCCAAGGAG 
GAAGAGCCAA GTGGCTGGGA GGAACCATCC TCACAATCCA TTGGTCATAA AATGGACATT 
GATGACGGAA CAGCAGCTTG GGGGGATCCT AACAATTACA ATTACAAAAA TGTCAGCCTG 
TGGGACAAGA ATGCTCAAGC AGGCCCTGGG CAGATGGCTC GGGAGCAAGG AATATCTGGG 
ACATTGGCAA ACAAGGCAGC TCCCTCAGTG TGGAGTAAAA ACAAAGGACC CCCAACTGAC 
AATGGGACAT CAGCTTGGGG TGAGCCAACT GAACCTGCTT CAGGGTGGGG AGAAGGTGAG 
GAGTCGGGTC CACCAACACC AGGATGGGGA AGTGCACCCC AAGTAAATCC CAGTGCCATG 
AAATCTGGTA CAAAATCTAT GCAAGATGGC TGGGGTGAGA GTGATGGGCC GGTGACAGGA 
ACACGTCACA GCAGCTGGGA TGAAGAAGAA GAGGGTGGAG TCTGGAACAG TGCAGGTTCT 
CAGGGAAGTG GCTCCTCCCA CAACTCTGCA GGTTGGGGAC AAGGAGGAAA GAAACCACAG 
ATCAAGGGTC CCTTAAAAGG AGGGAGCAAT GATACCTGGA TGAACACCTT GCCCAAACAG 
TTCTCCAACA TGGGATTGTT GAGCCAAGTT GATGATCCCT CTACCACTAA GATGGACCAT 
CCTGGCGGGC TTCCAGATAA GAAGTTTGAG ACAGACAAGC GGGGCGTGAA TCTCGGGGAT 
TTTAATGACA TGATGCGGAA GGAACGGTCT GGCTTCCGTC TGAACAATTC CAAAGAGATG 
GGAGCTGCAG ACAGTGGGCC TTATTTTGAG AAGCTGACTT TGCCTTTCTC CAATCAAGAC 
GGGTGCCTTG GGGATGAGGC TCCCTGTTCC CCTTTTTCTC CCTCCCCTAG CTACAAGCTA 
TCTCCCTCCG GCTCCTCGCT CCCTTCCGTG GGCCTGGGGG CTATCGGTTC TGGCCTCAAC 
CCCCAAACCT TCGCTGCTAG ACAAGGTGGC AATCACGGAC TATTTGGAAA CAGCGGTGCA 
CAATCGCGAG GCATGCACAA CCCTGTGCCA TCGCTCAACT CCTCTCCCAA CCTCCGGGCA 
CAAGTGCCTC CCCAGTTCCT CTCTCCCCAG GTCTCAGCCT CCATGCTAAA GCAGTTTCCT 
AACAGTAACA TGAATCCAGG ACTTTTCAAT ATGGGGCCAC AGCTTTCCCC TCAGCAGATT 
GCAATGCTGA GCCAGCTTCC TCAAATTCAG CAGTTCCAGC TGGCATGTCA GCTCCTCTTG 
CAACAACAAC AACAGCAGCA ACAGCAGCAG CAGCAGCAGC AACTCCTTCA GAACCAGAGG 
AAGATCTCAC AAGCGGTCAG ACAGCAGCAG GAACAGCAGC AGATAGCACG CATGGTAAGT 
GCCCTACAAC AGCAGCAACA GCAACAGCAG CAGCAGCAAC AGAGGCAGCC AGGAATGAAG 
CACTCTCCCT CTCACCCTGC TGGACCCAAA GGTCACTTAG ATGCTGTTGG ACTCTCAGAC 
ATGCAAGCCA AAGGGCACAT GCCATATGGA TCTGGTTTTG GCTCAAGTGG GATGGATTAT 
GGTACAGTAG GTAAGGAGGC GGGAGCAGAG TCTCGATTTA AACAGTGGAC ATCCATGATG 
GAGGGGCTAC CGTCCACTGT TTCTCAAGAT ACCAGCCTGC ATAAAAATGG CTCCATTGCA 
CCCCCAGTAA AAAATCGTGG AGGTTCCCCC TACCACCAAT ATGACATGTT GTCTACGGAT 
GCTTTGGGAG GGCATGGGGG GCCAACAGCT GAGAGCTGGT TACCTGCCAA ATCACCCCCT 
TCAAGCAAAA TTGGAAACAA GTCAATCAAT GCCAGCTGGC CCCCAGAGTT TCAGCCAGGA 
GTGCCATGGA AAGGGATCCA AAATATTGAT CCAGAATCTG ATCCTTATGT TACCCCTGGC 
AGTGTGCTTG GGGGTCCAGC CCCATCTCCC ATTGTAGATA CTGACCACCA GCTACTGCAG 
GACACCCCCA CAGGGTCCAA TTCTTCCCTC AACACCTCGC TGCCTTCACC TGGTGCCTGG 
CCCTTTAGTG CCTCTGAGAA TATCCATAGC ACTTCAGCAC TCTCTGCAAA GTTTGGGGAC 
TACAAATCGA CCTGGTCACC AGACCCCATA GGACATAACC CATCACATCA CTCAAACAAG 
ATGTGGAAAA ACCAGATGTC CAGGAACAGC CCTGGGCTGC CTCGCCCACC TCCAGGGCTC 
ACAAACAACC CCAAAGCCAC CAACTCTCCC TGGAACAGCA CAGCACCTCG CTCCGTCAGG 
GGCTGGGGCG GACAGGACTC CAGAATGGCT TCTGCATCAG CTTGGAGTGA TGGTGGATCT 
GTGCGCCCCA GCTACTGGCT GATCCTTCTT AATCTCACAC CACAGATCGA TGGTTCCACC 
CTGCGAACGA TCTGCATGCA GCATGGCCCC CTGCTGACAT TCCATCTCAA CCTTACCCAG 
GGCACAGCTC TAATCCGATA CAGCACCAAA CAGGAGGCAG CCAAGGCCCA GAGTGCCCTC 
CATATGTGTG TTCTGGGTAA TACAACTATC CTGGCGGAGT TTGCAACGGA TGAGGAGGTG 
AGCCGGTATC TTGCACAAGC TCAGCCACCA GCTCCCTCTG CTCCAGGAGC AGGCTGGCAG 
CCTTTGGAAG TGGGACCGAA CCACCCAGCT GAACCAGTAG GGCCAGCCCT AAACCTTTTT 
GGAGGAACCA CAGGGCTAGG GCAATGGAGC AGCGGCGGTG GTGGTAGTGA CCTGTCAGGG 
GTTTCTCTCT GGGGTACTCC TAGCAGCTAC ACATCCAGTC TGTGGGGTGT ACCGAGCACA 
GAGGACGCCC ACAGGATGGG CAGCCCTGCC CCGTTACTAC CCGGTGACCT TCTGGGAGGA 
GGGACCGACT CGATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816935.2
CDS	336..5873
Translation	MEDKKRKKEDKKKKEAAQKVTEQKTKVPEVTKPSSSQATAASLPGSSPSPPVNGGNNAKRVAVPNGQPPSTARYMPREVPPRFRCQQDHKVLLKRGQPPPASCMLPGGGAGTPPSTTAPGAGPGNAQPGAGTLLPGESGASTDSSSGAAATSNYANSTWGSGGPTSSGPTTNPPHAWDKVIVDGSDMEEWPCIAGVDAEPPSDNATDNNSASNSASEKGPPQGSTTSHKGRGGQGQQQQQQQPGSECIQGVWKSDPKAKPVPSSNPASEGVNRLGNWRNGGGSERAGSASGFSNFNPNTNPSAWPALVQEGNRKGNPESDSSFSGAQLGPVGLTSREQQSKMENAGSSFAGSGRESATTHHTDGPKNGNTNSTNSNTLNPPDNKGTAFGVGSMDSCRGSDSPAQSTGERKNGGIGSWGAGRGQSATGDANCGHSNSGSNGREREDARKAPSSHRPNGSRGESWESKSGNGSWGYSPQADLSEVKWSEGNKLGAGGASQGEWKQPAGHEDLKVGEWGGLNQGNSSTGAWDNQKGHSLSDNQGNTSSAACWGRAPSSAGSEAGGQSTGSNAKAGGSGGSGDSLNSSSRRSHRPALPDSQAALQTLLNRTDLDPRVLSNSGWGQTQIKQDASWESEEVPSRTEGKSDRGTEGWENVSQAKTSGGWGDAPSQSNQNKSGWGEPPVPAPSTQEWKDTKATGWNDYKNSPTGWVGGGGGGVVGARPEDKAVPGWNNESGGAAEPGWSTRQSNPGGGWSAGKNGWGGNGSEDMEPPKAPGGWEGSGSKSSSGWGEVGQNDVGSWGNGANSNPAPRTGWEETKRPQGGGNWGEAARPQLPWNKQQESTPSSATPSTWGGSQAPPPPGNGRSQPTSGWKGGPQPTVSKEEEPSGWEEPSSQSIGHKMDIDDGTAAWGDPNNYNYKNVSLWDKNAQAGPGQMAREQGISGTLANKAAPSVWSKNKGPPTDNGTSAWGEPTEPASGWGEGEESGPPTPGWGSAPQVNPSAMKSGTKSMQDGWGESDGPVTGTRHSSWDEEEEGGVWNSAGSQGSGSSHNSAGWGQGGKKPQIKGPLKGGSNDTWMNTLPKQFSNMGLLSQVDDPSTTKMDHPGGLPDKKFETDKRGVNLGDFNDMMRKERSGFRLNNSKEMGAADSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSSLPSVGLGAIGSGLNPQTFAARQGGNHGLFGNSGAQSRGMHNPVPSLNSSPNLRAQVPPQFLSPQVSASMLKQFPNSNMNPGLFNMGPQLSPQQIAMLSQLPQIQQFQLACQLLLQQQQQQQQQQQQQQLLQNQRKISQAVRQQQEQQQIARMVSALQQQQQQQQQQQQRQPGMKHSPSHPAGPKGHLDAVGLSDMQAKGHMPYGSGFGSSGMDYGTVGKEAGAESRFKQWTSMMEGLPSTVSQDTSLHKNGSIAPPVKNRGGSPYHQYDMLSTDALGGHGGPTAESWLPAKSPPSSKIGNKSINASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGPAPSPIVDTDHQLLQDTPTGSNSSLNTSLPSPGAWPFSASENIHSTSALSAKFGDYKSTWSPDPIGHNPSHHSNKMWKNQMSRNSPGLPRPPPGLTNNPKATNSPWNSTAPRSVRGWGGQDSRMASASAWSDGGSVRPSYWLILLNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQSALHMCVLGNTTILAEFATDEEVSRYLAQAQPPAPSAPGAGWQPLEVGPNHPAEPVGPALNLFGGTTGLGQWSSGGGGSDLSGVSLWGTPSSYTSSLWGVPSTEDAHRMGSPAPLLPGDLLGGGTDSI

Target ORF information:

RefSeq Version	XM_012961481.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961481.3

ORF Insert Sequence:

ATGGAAGACA AGAAAAGGAA GAAAGAGGAT AAAAAGAAGA AAGAAGCTGC TCAGAAGGTC 
ACAGAACAAA AAACCAAAGT GCCCGAAGTG ACGAAACCAA GTTCAAGCCA AGCGACGGCC 
GCCAGCCTAC CTGGCAGCTC CCCCTCGCCA CCAGTCAATG GTGGCAACAA TGCCAAAAGG 
GTGGCAGTGC CGAACGGACA ACCGCCCAGC ACCGCCCGCT ATATGCCTCG GGAGGTGCCG 
CCGCGATTCC GCTGCCAGCA GGACCACAAA GTGTTACTGA AGCGTGGACA GCCTCCTCCA 
GCGTCCTGTA TGCTGCCGGG GGGAGGAGCA GGGACTCCCC CCTCAACCAC TGCTCCAGGG 
GCCGGCCCAG GCAACGCACA GCCAGGGGCG GGGACACTGC TGCCAGGGGA AAGCGGGGCT 
TCAACAGACT CATCATCGGG GGCAGCTGCT ACTTCAAATT ATGCAAATTC CACTTGGGGC 
TCAGGAGGCC CCACCAGCAG CGGCCCCACC ACAAACCCTC CCCACGCCTG GGACAAAGTG 
ATTGTAGACG GGTCGGACAT GGAAGAGTGG CCTTGCATAG CCGGCGTGGA CGCAGAGCCC 
CCTTCCGACA ACGCCACAGA CAACAACAGT GCCTCGAACT CAGCCTCGGA GAAGGGACCC 
CCACAAGGAA GCACCACTAG CCACAAAGGG CGAGGGGGCC AGGGCCAGCA GCAACAGCAG 
CAGCAGCCTG GTAGTGAATG TATCCAGGGC GTCTGGAAAT CTGACCCCAA GGCTAAGCCC 
GTCCCGTCTT CCAACCCTGC TTCAGAGGGT GTAAATAGAC TGGGAAACTG GAGAAATGGG 
GGTGGGTCAG AGAGAGCAGG TTCTGCCTCT GGTTTCAGCA ACTTTAATCC AAATACTAAC 
CCATCTGCTT GGCCTGCCCT TGTCCAGGAA GGCAACAGGA AAGGGAACCC AGAGTCAGAC 
AGCAGCTTTT CTGGCGCACA ACTCGGCCCA GTGGGTTTGA CCTCTCGGGA GCAGCAGTCA 
AAGATGGAAA ATGCAGGTTC CAGCTTTGCA GGATCGGGCA GGGAGTCTGC CACAACCCAT 
CACACTGACG GACCAAAAAA TGGAAACACT AACTCTACGA ACTCAAATAC TTTGAACCCT 
CCTGATAACA AGGGAACAGC TTTTGGGGTG GGCTCAATGG ATTCCTGCCG GGGTTCAGAC 
TCCCCTGCTC AAAGCACTGG AGAAAGAAAG AATGGGGGGA TTGGCTCTTG GGGAGCGGGA 
AGGGGTCAGT CTGCCACAGG AGATGCTAAT TGTGGACATA GCAACTCTGG GAGCAATGGA 
CGAGAGAGAG AAGACGCAAG GAAAGCGCCT TCATCTCATA GACCCAATGG GTCAAGGGGG 
GAATCTTGGG AAAGCAAAAG TGGGAATGGT TCTTGGGGTT ACAGCCCGCA GGCAGACCTC 
AGTGAAGTGA AATGGAGTGA AGGGAACAAA TTGGGGGCTG GAGGGGCGTC TCAGGGAGAA 
TGGAAGCAGC CAGCCGGGCA TGAGGACTTA AAGGTGGGAG AGTGGGGAGG CTTGAACCAA 
GGAAACTCTA GCACTGGAGC ATGGGACAAT CAGAAGGGCC ACTCTCTATC GGATAACCAG 
GGCAACACGT CATCAGCAGC CTGCTGGGGC CGAGCCCCCA GCTCTGCAGG AAGTGAAGCT 
GGGGGGCAAA GCACTGGAAG CAACGCTAAA GCAGGGGGGA GCGGAGGAAG CGGAGACAGC 
CTGAACTCCA GCAGTCGACG TTCACACAGA CCTGCCCTCC CAGACAGTCA GGCTGCTCTT 
CAGACTTTAC TGAATCGCAC TGATTTGGAT CCCCGGGTGC TGTCCAACTC TGGCTGGGGC 
CAAACCCAGA TCAAGCAAGA TGCATCATGG GAGAGTGAGG AAGTGCCCTC TCGAACTGAA 
GGCAAATCAG ACCGTGGGAC TGAAGGTTGG GAGAACGTGT CCCAAGCCAA AACCTCAGGG 
GGTTGGGGGG ATGCTCCCAG CCAAAGCAAT CAAAACAAGT CAGGCTGGGG GGAGCCCCCT 
GTCCCTGCAC CTTCAACCCA AGAATGGAAG GACACCAAAG CAACTGGCTG GAATGACTAT 
AAAAACAGCC CAACTGGTTG GGTTGGTGGA GGAGGTGGTG GGGTGGTTGG AGCACGACCA 
GAGGACAAGG CAGTTCCAGG TTGGAACAAT GAAAGTGGTG GGGCTGCGGA GCCTGGGTGG 
AGTACTCGCC AGTCTAATCC AGGAGGTGGC TGGTCAGCAG GAAAGAATGG TTGGGGGGGA 
AATGGTAGTG AGGACATGGA GCCTCCCAAG GCCCCTGGAG GCTGGGAAGG CTCAGGAAGC 
AAATCCTCCT CTGGCTGGGG TGAAGTTGGA CAAAATGATG TGGGTTCTTG GGGTAATGGG 
GCAAACTCAA ATCCAGCTCC ACGAACTGGC TGGGAGGAGA CTAAGAGGCC CCAGGGAGGA 
GGAAACTGGG GTGAGGCAGC TAGGCCCCAA CTACCCTGGA ACAAACAGCA GGAATCAACG 
CCATCTTCAG CAACTCCTAG CACCTGGGGG GGCTCTCAGG CTCCCCCGCC ACCTGGAAAT 
GGGAGGTCAC AGCCAACTTC AGGGTGGAAG GGTGGTCCCC AGCCAACTGT TTCCAAGGAG 
GAAGAGCCAA GTGGCTGGGA GGAACCATCC TCACAATCCA TTGGTCATAA AATGGACATT 
GATGACGGAA CAGCAGCTTG GGGGGATCCT AACAATTACA ATTACAAAAA TGTCAGCCTG 
TGGGACAAGA ATGCTCAAGC AGGCCCTGGG CAGATGGCTC GGGAGCAAGG AATATCTGGG 
ACATTGGCAA ACAAGGCAGC TCCCTCAGTG TGGAGTAAAA ACAAAGGACC CCCAACTGAC 
AATGGGACAT CAGCTTGGGG TGAGCCAACT GAACCTGCTT CAGGGTGGGG AGAAGGTGAG 
GAGTCGGGTC CACCAACACC AGGATGGGGA AGTGCACCCC AAGTAAATCC CAGTGCCATG 
AAATCTGGTA CAAAATCTAT GCAAGATGGC TGGGGTGAGA GTGATGGGCC GGTGACAGGA 
ACACGTCACA GCAGCTGGGA TGAAGAAGAA GAGGGTGGAG TCTGGAACAG TGCAGGTTCT 
CAGGGAAGTG GCTCCTCCCA CAACTCTGCA GGTTGGGGAC AAGGAGGAAA GAAACCACAG 
ATCAAGGGTC CCTTAAAAGG AGGGAGCAAT GATACCTGGA TGAACACCTT GCCCAAACAG 
TTCTCCAACA TGGGATTGTT GAGCCAAGTT GATGATCCCT CTACCACTAA GATGGACCAT 
CCTGGCGGGC TTCCAGATAA GAAGTTTGAG ACAGACAAGC GGGGCGTGAA TCTCGGGGAT 
TTTAATGACA TGATGCGGAA GGAACGGTCT GGCTTCCGTC TGAACAATTC CAAAGAGATG 
GGAGCTGCAG ACAGTGGGCC TTATTTTGAG AAGCTGACTT TGCCTTTCTC CAATCAAGAC 
GGGTGCCTTG GGGATGAGGC TCCCTGTTCC CCTTTTTCTC CCTCCCCTAG CTACAAGCTA 
TCTCCCTCCG GCTCCTCGCT CCCTTCCGTG GGCCTGGGGG CTATCGGTTC TGGCCTCAAC 
CCCCAAACCT TCGCTGCTAG ACAAGGTGGC AATCACGGAC TATTTGGAAA CAGCGGTGCA 
CAATCGCGAG GCATGCACAA CCCTGTGCCA TCGCTCAACT CCTCTCCCAA CCTCCGGGCA 
CAAGTGCCTC CCCAGTTCCT CTCTCCCCAG GTCTCAGCCT CCATGCTAAA GCAGTTTCCT 
AACAGTAACA TGAATCCAGG ACTTTTCAAT ATGGGGCCAC AGCTTTCCCC TCAGCAGATT 
GCAATGCTGA GCCAGCTTCC TCAAATTCAG CAGTTCCAGC TGGCATGTCA GCTCCTCTTG 
CAACAACAAC AACAGCAGCA ACAGCAGCAG CAGCAGCAGC AACTCCTTCA GAACCAGAGG 
AAGATCTCAC AAGCGGTCAG ACAGCAGCAG GAACAGCAGC AGATAGCACG CATGGTAAGT 
GCCCTACAAC AGCAGCAACA GCAACAGCAG CAGCAGCAAC AGAGGCAGCC AGGAATGAAG 
CACTCTCCCT CTCACCCTGC TGGACCCAAA GGTCACTTAG ATGCTGTTGG ACTCTCAGAC 
ATGCAAGCCA AAGGGCACAT GCCATATGGA TCTGGTTTTG GCTCAAGTGG GATGGATTAT 
GGTACAGTAG GTAAGGAGGC GGGAGCAGAG TCTCGATTTA AACAGTGGAC ATCCATGATG 
GAGGGGCTAC CGTCCACTGT TTCTCAAGAT ACCAGCCTGC ATAAAAATGG CTCCATTGCA 
CCCCCAGTAA AAAATCGTGG AGGTTCCCCC TACCACCAAT ATGACATGTT GTCTACGGAT 
GCTTTGGGAG GGCATGGGGG GCCAACAGCT GAGAGCTGGT TACCTGCCAA ATCACCCCCT 
TCAAGCAAAA TTGGAAACAA GTCAATCAAT GCCAGCTGGC CCCCAGAGTT TCAGCCAGGA 
GTGCCATGGA AAGGGATCCA AAATATTGAT CCAGAATCTG ATCCTTATGT TACCCCTGGC 
AGTGTGCTTG GGGGTCCAGC CCCATCTCCC ATTGTAGATA CTGACCACCA GCTACTGCAG 
GACACCCCCA CAGGGTCCAA TTCTTCCCTC AACACCTCGC TGCCTTCACC TGGTGCCTGG 
CCCTTTAGTG CCTCTGAGAA TATCCATAGC ACTTCAGCAC TCTCTGCAAA GTTTGGGGAC 
TACAAATCGA CCTGGTCACC AGACCCCATA GGACATAACC CATCACATCA CTCAAACAAG 
ATGTGGAAAA ACCAGATGTC CAGGAACAGC CCTGGGCTGC CTCGCCCACC TCCAGGGCTC 
ACAAACAACC CCAAAGCCAC CAACTCTCCC TGGAACAGCA CAGCACCTCG CTCCGTCAGG 
GGCTGGGGCG GACAGGACTC CAGAATGGCT TCTGCATCAG CTTGGAGTGA TGGTGGATCT 
GTGCGCCCCA GCTACTGGCT GATCCTTCTT AATCTCACAC CACAGATCGA TGGTTCCACC 
CTGCGAACGA TCTGCATGCA GCATGGCCCC CTGCTGACAT TCCATCTCAA CCTTACCCAG 
GGCACAGCTC TAATCCGATA CAGCACCAAA CAGGAGGCAG CCAAGGCCCA GAGTGCCCTC 
CATATGTGTG TTCTGGGTAA TACAACTATC CTGGCGGAGT TTGCAACGGA TGAGGAGGTG 
AGCCGGTATC TTGCACAAGC TCAGCCACCA GCTCCCTCTG CTCCAGGAGC AGGCTGGCAG 
CCTTTGGAAG TGGGACCGAA CCACCCAGCT GAACCAGTAG GGCCAGCCCT AAACCTTTTT 
GGAGGAACCA CAGGGCTAGG GCAATGGAGC AGCGGCGGTG GTGGTAGTGA CCTGTCAGGG 
GTTTCTCTCT GGGGTACTCC TAGCAGCTAC ACATCCAGTC TGTGGGGTGT ACCGAGCACA 
GAGGACGCCC ACAGGATGGG CAGCCCTGCC CCGTTACTAC CCGGTGACCT TCTGGGAGGA 
GGGACCGACT CGATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnrc6b ( XM_012961478.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816932.1 Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X4, mRNA.

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CloneID	OXa09307
Clone ID Related Accession (Same CDS sequence)	XM_012961478.2 , XM_012961477.2 , XM_002934209.4
Accession Version	XM_012961478.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trinucleotide repeat-containing gene 6B protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961478.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAGA AACACTTGAA GAAAGCTGTT CCCAGAGAGA AGGAGCAAGA AAGGGAAGAA 
CAGTTAATGG AAGACAAGAA AAGGAAGAAA GAGGATAAAA AGAAGAAAGA AGCTGCTCAG 
AAGGTCACAG AACAAAAAAC CAAAGTGCCC GAAGTGACGA AACCAAGTTC AAGCCAAGCG 
ACGGCCGCCA GCCTACCTGG CAGCTCCCCC TCGCCACCAG TCAATGGTGG CAACAATGCC 
AAAAGGGTGG CAGTGCCGAA CGGACAACCG CCCAGCACCG CCCGCTATAT GCCTCGGGAG 
GTGCCGCCGC GATTCCGCTG CCAGCAGGAC CACAAAGTGT TACTGAAGCG TGGACAGCCT 
CCTCCAGCGT CCTGTATGCT GCCGGGGGGA GGAGCAGGGA CTCCCCCCTC AACCACTGCT 
CCAGGGGCCG GCCCAGGCAA CGCACAGCCA GGGGCGGGGA CACTGCTGCC AGGGGAAAGC 
GGGGCTTCAA CAGACTCATC ATCGGGGGCA GCTGCTACTT CAAATTATGC AAATTCCACT 
TGGGGCTCAG GAGGCCCCAC CAGCAGCGGC CCCACCACAA ACCCTCCCCA CGCCTGGGAC 
AAAGTGATTG TAGACGGGTC GGACATGGAA GAGTGGCCTT GCATAGCCGG CGTGGACGCA 
GAGCCCCCTT CCGACAACGC CACAGACAAC AACAGTGCCT CGAACTCAGC CTCGGAGAAG 
GGACCCCCAC AAGGAAGCAC CACTAGCCAC AAAGGGCGAG GGGGCCAGGG CCAGCAGCAA 
CAGCAGCAGC AGCCTGGTAG TGAATGTATC CAGGGCGTCT GGAAATCTGA CCCCAAGGCT 
AAGCCCGTCC CGTCTTCCAA CCCTGCTTCA GAGGGTGTAA ATAGACTGGG AAACTGGAGA 
AATGGGGGTG GGTCAGAGAG AGCAGGTTCT GCCTCTGGTT TCAGCAACTT TAATCCAAAT 
ACTAACCCAT CTGCTTGGCC TGCCCTTGTC CAGGAAGGCA ACAGGAAAGG GAACCCAGAG 
TCAGACAGCA GCTTTTCTGG CGCACAACTC GGCCCAGTGG GTTTGACCTC TCGGGAGCAG 
CAGTCAAAGA TGGAAAATGC AGGTTCCAGC TTTGCAGGAT CGGGCAGGGA GTCTGCCACA 
ACCCATCACA CTGACGGACC AAAAAATGGA AACACTAACT CTACGAACTC AAATACTTTG 
AACCCTCCTG ATAACAAGGG AACAGCTTTT GGGGTGGGCT CAATGGATTC CTGCCGGGGT 
TCAGACTCCC CTGCTCAAAG CACTGGAGAA AGAAAGAATG GGGGGATTGG CTCTTGGGGA 
GCGGGAAGGG GTCAGTCTGC CACAGGAGAT GCTAATTGTG GACATAGCAA CTCTGGGAGC 
AATGGACGAG AGAGAGAAGA CGCAAGGAAA GCGCCTTCAT CTCATAGACC CAATGGGTCA 
AGGGGGGAAT CTTGGGAAAG CAAAAGTGGG AATGGTTCTT GGGGTTACAG CCCGCAGGCA 
GACCTCAGTG AAGTGAAATG GAGTGAAGGG AACAAATTGG GGGCTGGAGG GGCGTCTCAG 
GGAGAATGGA AGCAGCCAGC CGGGCATGAG GACTTAAAGG TGGGAGAGTG GGGAGGCTTG 
AACCAAGGAA ACTCTAGCAC TGGAGCATGG GACAATCAGA AGGGCCACTC TCTATCGGAT 
AACCAGGGCA ACACGTCATC AGCAGCCTGC TGGGGCCGAG CCCCCAGCTC TGCAGGAAGT 
GAAGCTGGGG GGCAAAGCAC TGGAAGCAAC CCTAAAGCAG GGGGGAGCGG AGGAAGCGGA 
GACAGCCTGA ACTCCAGCAG TCGACGTTCA CACAGACCTG CCCTCCCAGA CAGTCAGGCT 
GCTCTTCAGA CTTTACTGAA TCGCACTGAT TTGGATCCCC GGGTGCTGTC CAACTCTGGC 
TGGGGCCAAA CCCAGATCAA GCAAGATGCA TCATGGGAGA GTGAGGAAGT GCCCTCTCGA 
ACTGAAGGCA AATCAGACCG TGGGACTGAA GGTTGGGAGA ACGTGTCCCA AGCCAAAACC 
TCAGGGGGTT GGGGGGATGC TCCCAGCCAA AGCAATCAAA ACAAGTCAGG CTGGGGGGAG 
CCCCCTGTCC CTGCACCTTC AACCCAAGAA TGGAAGGACA CCAAAGCAAC TGGCTGGAAT 
GACTATAAAA ACAGCCCAAC TGGTTGGGTT GGTGGAGGAG GTGGTGGGGT GGTTGGAGCA 
CGACCAGAGG ACAAGGCAGT TCCAGGTTGG AACAATGAAA GTGGTGGGGC TGCGGAGCCT 
GGGTGGAGTA CTCGCCAGTC TAATCCAGGA GGTGGCTGGT CAGCAGGAAA GAATGGTTGG 
GGGGGAAATG GTAGTGAGGA CATGGAGCCT CCCAAGGCCC CTGGAGGCTG GGAAGGCTCA 
GGAAGCAAAT CCTCCTCTGG CTGGGGTGAA GTTGGACAAA ATGATGTGGG TTCTTGGGGT 
AATGGGGCAA ACTCAAATCC AGCTCCACGA ACTGGCTGGG AGGAGACTAA GAGGCCCCAG 
GGAGGAGGAA ACTGGGGTGA GGCAGCTAGG CCCCAACTAC CCTGGAACAA ACAGCAGGAA 
TCAACGCCAT CTTCAGCAAC TCCTAGCACC TGGGGGGGCT CTCAGGCTCC CCCGCCACCT 
GGAAATGGGA GGTCACAGCC AACTTCAGGG TGGAAGGGTG GTCCCCAGCC AACTGTTTCC 
AAGGAGGAAG AGCCAAGTGG CTGGGAGGAA CCATCCTCAC AATCCATTGG TCATAAAATG 
GACATTGATG ACGGAACAGC AGCTTGGGGG GATCCTAACA ATTACAATTA CAAAAATGTC 
AGCCTGTGGG ACAAGAATGC TCAAGCAGGC CCTGGGCAGA TGGCTCGGGA GCAAGGAATA 
TCTGGGACAT TGGCAAACAA GGCAGCTCCC TCAGTGTGGA GTAAAAACAA AGGACCCCCA 
ACTGACAATG GGACATCAGC TTGGGGTGAG CCAACTGAAC CTGCTTCAGG GTGGGGAGAA 
GGTGAGGAGT CGGGTCCACC AACACCAGGA TGGGGAAGTG CACCCCAAGT AAATCCCAGT 
GCCATGAAAT CTGGTACAAA ATCTATGCAA GATGGCTGGG GTGAGAGTGA TGGGCCGGTG 
ACAGGAACAC GTCACAGCAG CTGGGATGAA GAAGAAGAGG GTGGAGTCTG GAACAGTGCA 
GGTTCTCAGG GAAGTGGCTC CTCCCACAAC TCTGCAGGTT GGGGACAAGG AGGAAAGAAA 
CCACAGATCA AGGGTCCCTT AAAAGGAGGG AGCAATGATA CCTGGATGAA CACCTTGCCC 
AAACAGTTCT CCAACATGGG ATTGTTGAGC CAAGTTGATG ACCCCTCTAC CACTAAGATG 
GACCATCCTG GCGGGCTTCC AGATAAGAAG TTTGAGACAG ACAAGCGGGG CGTGAATCTC 
GGGGATTTTA ATGACATGAT GCGGAAGGAA CGGTCTGGCT TCCGTCTGAA CAATTCCAAA 
GAGATGGGAG CTGCAGACAG TGGGCCTTAT TTTGAGAAGC TGACTTTGCC TTTCTCCAAT 
CAAGACGGGT GCCTTGGGGA TGAGGCTCCC TGTTCCCCTT TTTCTCCCTC CCCTAGCTAC 
AAGCTATCTC CCTCCGGCTC CTCGCTCCCT TCCGTGGGCC TGGGGGCTAT CGGTTCTGGC 
CTCAACCCCC AAACCTTCGC TGCTAGACAA GGTGGCAATC ACGGACTATT TGGAAACAGC 
GGTGCACAAT CGCGAGGCAT GCACAACCCT GTGCCATCGC TCAACTCCTC TCCCAACCTC 
CGGGCACAAG TGCCTCCCCA GTTCCTCTCT CCCCAGGTCT CAGCCTCCAT GCTAAAGCAG 
TTTCCTAACA GTAACATGAA TCCAGGACTT TTCAATATGG GGCCACAGCT TTCCCCTCAG 
CAGATTGCAA TGCTGAGCCA GCTTCCTCAA ATTCAGCAGT TCCAGCTGGC ATGTCAGCTC 
CTCTTGCAAC AACAACAACA GCAGCAGCAA CAGCAGCAGC AGCAGCAGCA ACTCCTTCAG 
AACCAGAGAA AGATCTCACA AGCGGTCAGA CAGCAGCAGG AACAGCAGCA GATAGCACGC 
ATGGTAAGTG CCCTACAACA GCAGCAACAG CAACAGCAGC AGCAGCAACA GAGGCAGCCA 
GGAATGAAGC ACTCTCCCTC TCACCCTGCT GGACCCAAAG GTCACTTAGA TGCTGTTGGA 
CTCTCAGACA TGCAAGCCAA AGGGCACATG CCATATGGAT CTGGTTTTGG CTCAAGTGGG 
ATGGATTATG GTACAGTAGG TAAGGAGGCG GGAGCAGAGT CTCGATTTAA ACAGTGGACA 
TCCATGATGG AGGGGCTACC GTCCACTGTT TCTCAAGATA CCAGCCTGCA TAAAAATGGC 
TCCATTGCAC CCCCAGTAAA AAATCGTGGA GGTTCCCCCT ACCACCAATA TGACATGTTG 
TCTACGGATG CTTTGGGAGG GCATGGGGGG CCAACAGCTG AGAGCTGGTT ACCTGCCAAA 
TCACCCCCTT CAAGCAAAAT TGGAAACAAG TCAATCAATG CCAGCTGGCC CCCAGAGTTT 
CAGCCAGGAG TGCCATGGAA AGGGATCCAA AATATTGATC CAGAATCTGA TCCTTATGTT 
ACCCCTGGCA GTGTGCTTGG GGGTCCAGCC CCATCTCCCA TTGTAGATAC TGACCACCAG 
CTACTGCAGG ACACCCCCAC AGGGTCCAAT TCTTCCCTCA ACACCTCGCT GCCTTCACCT 
GGTGCCTGGC CCTTTAGTGC CTCTGAGAAT ATCCATAGCA CTTCAGCACT CTCTGCAAAG 
TTTGGGGACT ACAAATCGAC CTGGTCACCA GACCCCATAG GACATAACCC ATCACATCAC 
TCAAACAAGA TGTGGAAAAA CCAGATGTCC AGGAACAGCC CTGGGCTGCC TCGCCCACCT 
CCAGGGCTCA CAAACAACCC CAAAGCCACC AACTCTCCCT GGAACAGCAC AGCACCTCGC 
TCCGTCAGGG GCTGGGGCGG ACAGGACTCC AGAATGGCTT CTGCATCAGC TTGGAGTGAT 
GGTGGATCTG TGCGCCCCAG CTACTGGCTG ATCCTTCTTA ATCTCACACC ACAGATCGAT 
GGTTCCACCC TGCGAACGAT CTGCATGCAG CATGGCCCCC TGCTGACATT CCATCTCAAC 
CTTACCCAGG GCACAGCTCT AATCCGATAC AGCACCAAAC AGGAGGCAGC CAAGGCCCAG 
AGTGCCCTCC ATATGTGTGT TCTGGGTAAT ACAACTATCC TGGCGGAGTT TGCAACGGAT 
GAGGAGGTGA GCCGGTATCT TGCACAAGCT CAGCCACCAG CTCCCTCTGC TCCAGGAGCA 
GGCTGGCAGC CTTTGGAAGT GGGACCGAAC CACCCAGCTG AACCAGTAGG GCCAGCCCTA 
AACCTTTTTG GAGGAACCAC AGGGCTAGGG CAATGGAGCA GCGGCGGTGG TGGTAGTGAC 
CTGTCAGGGG TTTCTCTCTG GGGTACTCCT AGCAGCTACA CATCCAGTCT GTGGGGTGTG 
CCGAGCACAG AGGACGCCCA CAGGATGGGC AGCCCTGCCC CGTTACTACC CGGTGACCTT 
CTGGGAGGAG GGACCGACTC GATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816932.1
CDS	385..5991
Translation	MREKHLKKAVPREKEQEREEQLMEDKKRKKEDKKKKEAAQKVTEQKTKVPEVTKPSSSQATAASLPGSSPSPPVNGGNNAKRVAVPNGQPPSTARYMPREVPPRFRCQQDHKVLLKRGQPPPASCMLPGGGAGTPPSTTAPGAGPGNAQPGAGTLLPGESGASTDSSSGAAATSNYANSTWGSGGPTSSGPTTNPPHAWDKVIVDGSDMEEWPCIAGVDAEPPSDNATDNNSASNSASEKGPPQGSTTSHKGRGGQGQQQQQQQPGSECIQGVWKSDPKAKPVPSSNPASEGVNRLGNWRNGGGSERAGSASGFSNFNPNTNPSAWPALVQEGNRKGNPESDSSFSGAQLGPVGLTSREQQSKMENAGSSFAGSGRESATTHHTDGPKNGNTNSTNSNTLNPPDNKGTAFGVGSMDSCRGSDSPAQSTGERKNGGIGSWGAGRGQSATGDANCGHSNSGSNGREREDARKAPSSHRPNGSRGESWESKSGNGSWGYSPQADLSEVKWSEGNKLGAGGASQGEWKQPAGHEDLKVGEWGGLNQGNSSTGAWDNQKGHSLSDNQGNTSSAACWGRAPSSAGSEAGGQSTGSNPKAGGSGGSGDSLNSSSRRSHRPALPDSQAALQTLLNRTDLDPRVLSNSGWGQTQIKQDASWESEEVPSRTEGKSDRGTEGWENVSQAKTSGGWGDAPSQSNQNKSGWGEPPVPAPSTQEWKDTKATGWNDYKNSPTGWVGGGGGGVVGARPEDKAVPGWNNESGGAAEPGWSTRQSNPGGGWSAGKNGWGGNGSEDMEPPKAPGGWEGSGSKSSSGWGEVGQNDVGSWGNGANSNPAPRTGWEETKRPQGGGNWGEAARPQLPWNKQQESTPSSATPSTWGGSQAPPPPGNGRSQPTSGWKGGPQPTVSKEEEPSGWEEPSSQSIGHKMDIDDGTAAWGDPNNYNYKNVSLWDKNAQAGPGQMAREQGISGTLANKAAPSVWSKNKGPPTDNGTSAWGEPTEPASGWGEGEESGPPTPGWGSAPQVNPSAMKSGTKSMQDGWGESDGPVTGTRHSSWDEEEEGGVWNSAGSQGSGSSHNSAGWGQGGKKPQIKGPLKGGSNDTWMNTLPKQFSNMGLLSQVDDPSTTKMDHPGGLPDKKFETDKRGVNLGDFNDMMRKERSGFRLNNSKEMGAADSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSSLPSVGLGAIGSGLNPQTFAARQGGNHGLFGNSGAQSRGMHNPVPSLNSSPNLRAQVPPQFLSPQVSASMLKQFPNSNMNPGLFNMGPQLSPQQIAMLSQLPQIQQFQLACQLLLQQQQQQQQQQQQQQQLLQNQRKISQAVRQQQEQQQIARMVSALQQQQQQQQQQQQRQPGMKHSPSHPAGPKGHLDAVGLSDMQAKGHMPYGSGFGSSGMDYGTVGKEAGAESRFKQWTSMMEGLPSTVSQDTSLHKNGSIAPPVKNRGGSPYHQYDMLSTDALGGHGGPTAESWLPAKSPPSSKIGNKSINASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGPAPSPIVDTDHQLLQDTPTGSNSSLNTSLPSPGAWPFSASENIHSTSALSAKFGDYKSTWSPDPIGHNPSHHSNKMWKNQMSRNSPGLPRPPPGLTNNPKATNSPWNSTAPRSVRGWGGQDSRMASASAWSDGGSVRPSYWLILLNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQSALHMCVLGNTTILAEFATDEEVSRYLAQAQPPAPSAPGAGWQPLEVGPNHPAEPVGPALNLFGGTTGLGQWSSGGGGSDLSGVSLWGTPSSYTSSLWGVPSTEDAHRMGSPAPLLPGDLLGGGTDSI

Target ORF information:

RefSeq Version	XM_012961478.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961478.2

ORF Insert Sequence:

ATGAGGGAGA AACACTTGAA GAAAGCTGTT CCCAGAGAGA AGGAGCAAGA AAGGGAAGAA 
CAGTTAATGG AAGACAAGAA AAGGAAGAAA GAGGATAAAA AGAAGAAAGA AGCTGCTCAG 
AAGGTCACAG AACAAAAAAC CAAAGTGCCC GAAGTGACGA AACCAAGTTC AAGCCAAGCG 
ACGGCCGCCA GCCTACCTGG CAGCTCCCCC TCGCCACCAG TCAATGGTGG CAACAATGCC 
AAAAGGGTGG CAGTGCCGAA CGGACAACCG CCCAGCACCG CCCGCTATAT GCCTCGGGAG 
GTGCCGCCGC GATTCCGCTG CCAGCAGGAC CACAAAGTGT TACTGAAGCG TGGACAGCCT 
CCTCCAGCGT CCTGTATGCT GCCGGGGGGA GGAGCAGGGA CTCCCCCCTC AACCACTGCT 
CCAGGGGCCG GCCCAGGCAA CGCACAGCCA GGGGCGGGGA CACTGCTGCC AGGGGAAAGC 
GGGGCTTCAA CAGACTCATC ATCGGGGGCA GCTGCTACTT CAAATTATGC AAATTCCACT 
TGGGGCTCAG GAGGCCCCAC CAGCAGCGGC CCCACCACAA ACCCTCCCCA CGCCTGGGAC 
AAAGTGATTG TAGACGGGTC GGACATGGAA GAGTGGCCTT GCATAGCCGG CGTGGACGCA 
GAGCCCCCTT CCGACAACGC CACAGACAAC AACAGTGCCT CGAACTCAGC CTCGGAGAAG 
GGACCCCCAC AAGGAAGCAC CACTAGCCAC AAAGGGCGAG GGGGCCAGGG CCAGCAGCAA 
CAGCAGCAGC AGCCTGGTAG TGAATGTATC CAGGGCGTCT GGAAATCTGA CCCCAAGGCT 
AAGCCCGTCC CGTCTTCCAA CCCTGCTTCA GAGGGTGTAA ATAGACTGGG AAACTGGAGA 
AATGGGGGTG GGTCAGAGAG AGCAGGTTCT GCCTCTGGTT TCAGCAACTT TAATCCAAAT 
ACTAACCCAT CTGCTTGGCC TGCCCTTGTC CAGGAAGGCA ACAGGAAAGG GAACCCAGAG 
TCAGACAGCA GCTTTTCTGG CGCACAACTC GGCCCAGTGG GTTTGACCTC TCGGGAGCAG 
CAGTCAAAGA TGGAAAATGC AGGTTCCAGC TTTGCAGGAT CGGGCAGGGA GTCTGCCACA 
ACCCATCACA CTGACGGACC AAAAAATGGA AACACTAACT CTACGAACTC AAATACTTTG 
AACCCTCCTG ATAACAAGGG AACAGCTTTT GGGGTGGGCT CAATGGATTC CTGCCGGGGT 
TCAGACTCCC CTGCTCAAAG CACTGGAGAA AGAAAGAATG GGGGGATTGG CTCTTGGGGA 
GCGGGAAGGG GTCAGTCTGC CACAGGAGAT GCTAATTGTG GACATAGCAA CTCTGGGAGC 
AATGGACGAG AGAGAGAAGA CGCAAGGAAA GCGCCTTCAT CTCATAGACC CAATGGGTCA 
AGGGGGGAAT CTTGGGAAAG CAAAAGTGGG AATGGTTCTT GGGGTTACAG CCCGCAGGCA 
GACCTCAGTG AAGTGAAATG GAGTGAAGGG AACAAATTGG GGGCTGGAGG GGCGTCTCAG 
GGAGAATGGA AGCAGCCAGC CGGGCATGAG GACTTAAAGG TGGGAGAGTG GGGAGGCTTG 
AACCAAGGAA ACTCTAGCAC TGGAGCATGG GACAATCAGA AGGGCCACTC TCTATCGGAT 
AACCAGGGCA ACACGTCATC AGCAGCCTGC TGGGGCCGAG CCCCCAGCTC TGCAGGAAGT 
GAAGCTGGGG GGCAAAGCAC TGGAAGCAAC CCTAAAGCAG GGGGGAGCGG AGGAAGCGGA 
GACAGCCTGA ACTCCAGCAG TCGACGTTCA CACAGACCTG CCCTCCCAGA CAGTCAGGCT 
GCTCTTCAGA CTTTACTGAA TCGCACTGAT TTGGATCCCC GGGTGCTGTC CAACTCTGGC 
TGGGGCCAAA CCCAGATCAA GCAAGATGCA TCATGGGAGA GTGAGGAAGT GCCCTCTCGA 
ACTGAAGGCA AATCAGACCG TGGGACTGAA GGTTGGGAGA ACGTGTCCCA AGCCAAAACC 
TCAGGGGGTT GGGGGGATGC TCCCAGCCAA AGCAATCAAA ACAAGTCAGG CTGGGGGGAG 
CCCCCTGTCC CTGCACCTTC AACCCAAGAA TGGAAGGACA CCAAAGCAAC TGGCTGGAAT 
GACTATAAAA ACAGCCCAAC TGGTTGGGTT GGTGGAGGAG GTGGTGGGGT GGTTGGAGCA 
CGACCAGAGG ACAAGGCAGT TCCAGGTTGG AACAATGAAA GTGGTGGGGC TGCGGAGCCT 
GGGTGGAGTA CTCGCCAGTC TAATCCAGGA GGTGGCTGGT CAGCAGGAAA GAATGGTTGG 
GGGGGAAATG GTAGTGAGGA CATGGAGCCT CCCAAGGCCC CTGGAGGCTG GGAAGGCTCA 
GGAAGCAAAT CCTCCTCTGG CTGGGGTGAA GTTGGACAAA ATGATGTGGG TTCTTGGGGT 
AATGGGGCAA ACTCAAATCC AGCTCCACGA ACTGGCTGGG AGGAGACTAA GAGGCCCCAG 
GGAGGAGGAA ACTGGGGTGA GGCAGCTAGG CCCCAACTAC CCTGGAACAA ACAGCAGGAA 
TCAACGCCAT CTTCAGCAAC TCCTAGCACC TGGGGGGGCT CTCAGGCTCC CCCGCCACCT 
GGAAATGGGA GGTCACAGCC AACTTCAGGG TGGAAGGGTG GTCCCCAGCC AACTGTTTCC 
AAGGAGGAAG AGCCAAGTGG CTGGGAGGAA CCATCCTCAC AATCCATTGG TCATAAAATG 
GACATTGATG ACGGAACAGC AGCTTGGGGG GATCCTAACA ATTACAATTA CAAAAATGTC 
AGCCTGTGGG ACAAGAATGC TCAAGCAGGC CCTGGGCAGA TGGCTCGGGA GCAAGGAATA 
TCTGGGACAT TGGCAAACAA GGCAGCTCCC TCAGTGTGGA GTAAAAACAA AGGACCCCCA 
ACTGACAATG GGACATCAGC TTGGGGTGAG CCAACTGAAC CTGCTTCAGG GTGGGGAGAA 
GGTGAGGAGT CGGGTCCACC AACACCAGGA TGGGGAAGTG CACCCCAAGT AAATCCCAGT 
GCCATGAAAT CTGGTACAAA ATCTATGCAA GATGGCTGGG GTGAGAGTGA TGGGCCGGTG 
ACAGGAACAC GTCACAGCAG CTGGGATGAA GAAGAAGAGG GTGGAGTCTG GAACAGTGCA 
GGTTCTCAGG GAAGTGGCTC CTCCCACAAC TCTGCAGGTT GGGGACAAGG AGGAAAGAAA 
CCACAGATCA AGGGTCCCTT AAAAGGAGGG AGCAATGATA CCTGGATGAA CACCTTGCCC 
AAACAGTTCT CCAACATGGG ATTGTTGAGC CAAGTTGATG ACCCCTCTAC CACTAAGATG 
GACCATCCTG GCGGGCTTCC AGATAAGAAG TTTGAGACAG ACAAGCGGGG CGTGAATCTC 
GGGGATTTTA ATGACATGAT GCGGAAGGAA CGGTCTGGCT TCCGTCTGAA CAATTCCAAA 
GAGATGGGAG CTGCAGACAG TGGGCCTTAT TTTGAGAAGC TGACTTTGCC TTTCTCCAAT 
CAAGACGGGT GCCTTGGGGA TGAGGCTCCC TGTTCCCCTT TTTCTCCCTC CCCTAGCTAC 
AAGCTATCTC CCTCCGGCTC CTCGCTCCCT TCCGTGGGCC TGGGGGCTAT CGGTTCTGGC 
CTCAACCCCC AAACCTTCGC TGCTAGACAA GGTGGCAATC ACGGACTATT TGGAAACAGC 
GGTGCACAAT CGCGAGGCAT GCACAACCCT GTGCCATCGC TCAACTCCTC TCCCAACCTC 
CGGGCACAAG TGCCTCCCCA GTTCCTCTCT CCCCAGGTCT CAGCCTCCAT GCTAAAGCAG 
TTTCCTAACA GTAACATGAA TCCAGGACTT TTCAATATGG GGCCACAGCT TTCCCCTCAG 
CAGATTGCAA TGCTGAGCCA GCTTCCTCAA ATTCAGCAGT TCCAGCTGGC ATGTCAGCTC 
CTCTTGCAAC AACAACAACA GCAGCAGCAA CAGCAGCAGC AGCAGCAGCA ACTCCTTCAG 
AACCAGAGAA AGATCTCACA AGCGGTCAGA CAGCAGCAGG AACAGCAGCA GATAGCACGC 
ATGGTAAGTG CCCTACAACA GCAGCAACAG CAACAGCAGC AGCAGCAACA GAGGCAGCCA 
GGAATGAAGC ACTCTCCCTC TCACCCTGCT GGACCCAAAG GTCACTTAGA TGCTGTTGGA 
CTCTCAGACA TGCAAGCCAA AGGGCACATG CCATATGGAT CTGGTTTTGG CTCAAGTGGG 
ATGGATTATG GTACAGTAGG TAAGGAGGCG GGAGCAGAGT CTCGATTTAA ACAGTGGACA 
TCCATGATGG AGGGGCTACC GTCCACTGTT TCTCAAGATA CCAGCCTGCA TAAAAATGGC 
TCCATTGCAC CCCCAGTAAA AAATCGTGGA GGTTCCCCCT ACCACCAATA TGACATGTTG 
TCTACGGATG CTTTGGGAGG GCATGGGGGG CCAACAGCTG AGAGCTGGTT ACCTGCCAAA 
TCACCCCCTT CAAGCAAAAT TGGAAACAAG TCAATCAATG CCAGCTGGCC CCCAGAGTTT 
CAGCCAGGAG TGCCATGGAA AGGGATCCAA AATATTGATC CAGAATCTGA TCCTTATGTT 
ACCCCTGGCA GTGTGCTTGG GGGTCCAGCC CCATCTCCCA TTGTAGATAC TGACCACCAG 
CTACTGCAGG ACACCCCCAC AGGGTCCAAT TCTTCCCTCA ACACCTCGCT GCCTTCACCT 
GGTGCCTGGC CCTTTAGTGC CTCTGAGAAT ATCCATAGCA CTTCAGCACT CTCTGCAAAG 
TTTGGGGACT ACAAATCGAC CTGGTCACCA GACCCCATAG GACATAACCC ATCACATCAC 
TCAAACAAGA TGTGGAAAAA CCAGATGTCC AGGAACAGCC CTGGGCTGCC TCGCCCACCT 
CCAGGGCTCA CAAACAACCC CAAAGCCACC AACTCTCCCT GGAACAGCAC AGCACCTCGC 
TCCGTCAGGG GCTGGGGCGG ACAGGACTCC AGAATGGCTT CTGCATCAGC TTGGAGTGAT 
GGTGGATCTG TGCGCCCCAG CTACTGGCTG ATCCTTCTTA ATCTCACACC ACAGATCGAT 
GGTTCCACCC TGCGAACGAT CTGCATGCAG CATGGCCCCC TGCTGACATT CCATCTCAAC 
CTTACCCAGG GCACAGCTCT AATCCGATAC AGCACCAAAC AGGAGGCAGC CAAGGCCCAG 
AGTGCCCTCC ATATGTGTGT TCTGGGTAAT ACAACTATCC TGGCGGAGTT TGCAACGGAT 
GAGGAGGTGA GCCGGTATCT TGCACAAGCT CAGCCACCAG CTCCCTCTGC TCCAGGAGCA 
GGCTGGCAGC CTTTGGAAGT GGGACCGAAC CACCCAGCTG AACCAGTAGG GCCAGCCCTA 
AACCTTTTTG GAGGAACCAC AGGGCTAGGG CAATGGAGCA GCGGCGGTGG TGGTAGTGAC 
CTGTCAGGGG TTTCTCTCTG GGGTACTCCT AGCAGCTACA CATCCAGTCT GTGGGGTGTG 
CCGAGCACAG AGGACGCCCA CAGGATGGGC AGCCCTGCCC CGTTACTACC CGGTGACCTT 
CTGGGAGGAG GGACCGACTC GATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnrc6b ( XM_012961477.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816931.1 Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X3, mRNA.

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CloneID	OXa09307
Clone ID Related Accession (Same CDS sequence)	XM_012961478.2 , XM_012961477.2 , XM_002934209.4
Accession Version	XM_012961477.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trinucleotide repeat-containing gene 6B protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961477.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAGA AACACTTGAA GAAAGCTGTT CCCAGAGAGA AGGAGCAAGA AAGGGAAGAA 
CAGTTAATGG AAGACAAGAA AAGGAAGAAA GAGGATAAAA AGAAGAAAGA AGCTGCTCAG 
AAGGTCACAG AACAAAAAAC CAAAGTGCCC GAAGTGACGA AACCAAGTTC AAGCCAAGCG 
ACGGCCGCCA GCCTACCTGG CAGCTCCCCC TCGCCACCAG TCAATGGTGG CAACAATGCC 
AAAAGGGTGG CAGTGCCGAA CGGACAACCG CCCAGCACCG CCCGCTATAT GCCTCGGGAG 
GTGCCGCCGC GATTCCGCTG CCAGCAGGAC CACAAAGTGT TACTGAAGCG TGGACAGCCT 
CCTCCAGCGT CCTGTATGCT GCCGGGGGGA GGAGCAGGGA CTCCCCCCTC AACCACTGCT 
CCAGGGGCCG GCCCAGGCAA CGCACAGCCA GGGGCGGGGA CACTGCTGCC AGGGGAAAGC 
GGGGCTTCAA CAGACTCATC ATCGGGGGCA GCTGCTACTT CAAATTATGC AAATTCCACT 
TGGGGCTCAG GAGGCCCCAC CAGCAGCGGC CCCACCACAA ACCCTCCCCA CGCCTGGGAC 
AAAGTGATTG TAGACGGGTC GGACATGGAA GAGTGGCCTT GCATAGCCGG CGTGGACGCA 
GAGCCCCCTT CCGACAACGC CACAGACAAC AACAGTGCCT CGAACTCAGC CTCGGAGAAG 
GGACCCCCAC AAGGAAGCAC CACTAGCCAC AAAGGGCGAG GGGGCCAGGG CCAGCAGCAA 
CAGCAGCAGC AGCCTGGTAG TGAATGTATC CAGGGCGTCT GGAAATCTGA CCCCAAGGCT 
AAGCCCGTCC CGTCTTCCAA CCCTGCTTCA GAGGGTGTAA ATAGACTGGG AAACTGGAGA 
AATGGGGGTG GGTCAGAGAG AGCAGGTTCT GCCTCTGGTT TCAGCAACTT TAATCCAAAT 
ACTAACCCAT CTGCTTGGCC TGCCCTTGTC CAGGAAGGCA ACAGGAAAGG GAACCCAGAG 
TCAGACAGCA GCTTTTCTGG CGCACAACTC GGCCCAGTGG GTTTGACCTC TCGGGAGCAG 
CAGTCAAAGA TGGAAAATGC AGGTTCCAGC TTTGCAGGAT CGGGCAGGGA GTCTGCCACA 
ACCCATCACA CTGACGGACC AAAAAATGGA AACACTAACT CTACGAACTC AAATACTTTG 
AACCCTCCTG ATAACAAGGG AACAGCTTTT GGGGTGGGCT CAATGGATTC CTGCCGGGGT 
TCAGACTCCC CTGCTCAAAG CACTGGAGAA AGAAAGAATG GGGGGATTGG CTCTTGGGGA 
GCGGGAAGGG GTCAGTCTGC CACAGGAGAT GCTAATTGTG GACATAGCAA CTCTGGGAGC 
AATGGACGAG AGAGAGAAGA CGCAAGGAAA GCGCCTTCAT CTCATAGACC CAATGGGTCA 
AGGGGGGAAT CTTGGGAAAG CAAAAGTGGG AATGGTTCTT GGGGTTACAG CCCGCAGGCA 
GACCTCAGTG AAGTGAAATG GAGTGAAGGG AACAAATTGG GGGCTGGAGG GGCGTCTCAG 
GGAGAATGGA AGCAGCCAGC CGGGCATGAG GACTTAAAGG TGGGAGAGTG GGGAGGCTTG 
AACCAAGGAA ACTCTAGCAC TGGAGCATGG GACAATCAGA AGGGCCACTC TCTATCGGAT 
AACCAGGGCA ACACGTCATC AGCAGCCTGC TGGGGCCGAG CCCCCAGCTC TGCAGGAAGT 
GAAGCTGGGG GGCAAAGCAC TGGAAGCAAC CCTAAAGCAG GGGGGAGCGG AGGAAGCGGA 
GACAGCCTGA ACTCCAGCAG TCGACGTTCA CACAGACCTG CCCTCCCAGA CAGTCAGGCT 
GCTCTTCAGA CTTTACTGAA TCGCACTGAT TTGGATCCCC GGGTGCTGTC CAACTCTGGC 
TGGGGCCAAA CCCAGATCAA GCAAGATGCA TCATGGGAGA GTGAGGAAGT GCCCTCTCGA 
ACTGAAGGCA AATCAGACCG TGGGACTGAA GGTTGGGAGA ACGTGTCCCA AGCCAAAACC 
TCAGGGGGTT GGGGGGATGC TCCCAGCCAA AGCAATCAAA ACAAGTCAGG CTGGGGGGAG 
CCCCCTGTCC CTGCACCTTC AACCCAAGAA TGGAAGGACA CCAAAGCAAC TGGCTGGAAT 
GACTATAAAA ACAGCCCAAC TGGTTGGGTT GGTGGAGGAG GTGGTGGGGT GGTTGGAGCA 
CGACCAGAGG ACAAGGCAGT TCCAGGTTGG AACAATGAAA GTGGTGGGGC TGCGGAGCCT 
GGGTGGAGTA CTCGCCAGTC TAATCCAGGA GGTGGCTGGT CAGCAGGAAA GAATGGTTGG 
GGGGGAAATG GTAGTGAGGA CATGGAGCCT CCCAAGGCCC CTGGAGGCTG GGAAGGCTCA 
GGAAGCAAAT CCTCCTCTGG CTGGGGTGAA GTTGGACAAA ATGATGTGGG TTCTTGGGGT 
AATGGGGCAA ACTCAAATCC AGCTCCACGA ACTGGCTGGG AGGAGACTAA GAGGCCCCAG 
GGAGGAGGAA ACTGGGGTGA GGCAGCTAGG CCCCAACTAC CCTGGAACAA ACAGCAGGAA 
TCAACGCCAT CTTCAGCAAC TCCTAGCACC TGGGGGGGCT CTCAGGCTCC CCCGCCACCT 
GGAAATGGGA GGTCACAGCC AACTTCAGGG TGGAAGGGTG GTCCCCAGCC AACTGTTTCC 
AAGGAGGAAG AGCCAAGTGG CTGGGAGGAA CCATCCTCAC AATCCATTGG TCATAAAATG 
GACATTGATG ACGGAACAGC AGCTTGGGGG GATCCTAACA ATTACAATTA CAAAAATGTC 
AGCCTGTGGG ACAAGAATGC TCAAGCAGGC CCTGGGCAGA TGGCTCGGGA GCAAGGAATA 
TCTGGGACAT TGGCAAACAA GGCAGCTCCC TCAGTGTGGA GTAAAAACAA AGGACCCCCA 
ACTGACAATG GGACATCAGC TTGGGGTGAG CCAACTGAAC CTGCTTCAGG GTGGGGAGAA 
GGTGAGGAGT CGGGTCCACC AACACCAGGA TGGGGAAGTG CACCCCAAGT AAATCCCAGT 
GCCATGAAAT CTGGTACAAA ATCTATGCAA GATGGCTGGG GTGAGAGTGA TGGGCCGGTG 
ACAGGAACAC GTCACAGCAG CTGGGATGAA GAAGAAGAGG GTGGAGTCTG GAACAGTGCA 
GGTTCTCAGG GAAGTGGCTC CTCCCACAAC TCTGCAGGTT GGGGACAAGG AGGAAAGAAA 
CCACAGATCA AGGGTCCCTT AAAAGGAGGG AGCAATGATA CCTGGATGAA CACCTTGCCC 
AAACAGTTCT CCAACATGGG ATTGTTGAGC CAAGTTGATG ACCCCTCTAC CACTAAGATG 
GACCATCCTG GCGGGCTTCC AGATAAGAAG TTTGAGACAG ACAAGCGGGG CGTGAATCTC 
GGGGATTTTA ATGACATGAT GCGGAAGGAA CGGTCTGGCT TCCGTCTGAA CAATTCCAAA 
GAGATGGGAG CTGCAGACAG TGGGCCTTAT TTTGAGAAGC TGACTTTGCC TTTCTCCAAT 
CAAGACGGGT GCCTTGGGGA TGAGGCTCCC TGTTCCCCTT TTTCTCCCTC CCCTAGCTAC 
AAGCTATCTC CCTCCGGCTC CTCGCTCCCT TCCGTGGGCC TGGGGGCTAT CGGTTCTGGC 
CTCAACCCCC AAACCTTCGC TGCTAGACAA GGTGGCAATC ACGGACTATT TGGAAACAGC 
GGTGCACAAT CGCGAGGCAT GCACAACCCT GTGCCATCGC TCAACTCCTC TCCCAACCTC 
CGGGCACAAG TGCCTCCCCA GTTCCTCTCT CCCCAGGTCT CAGCCTCCAT GCTAAAGCAG 
TTTCCTAACA GTAACATGAA TCCAGGACTT TTCAATATGG GGCCACAGCT TTCCCCTCAG 
CAGATTGCAA TGCTGAGCCA GCTTCCTCAA ATTCAGCAGT TCCAGCTGGC ATGTCAGCTC 
CTCTTGCAAC AACAACAACA GCAGCAGCAA CAGCAGCAGC AGCAGCAGCA ACTCCTTCAG 
AACCAGAGAA AGATCTCACA AGCGGTCAGA CAGCAGCAGG AACAGCAGCA GATAGCACGC 
ATGGTAAGTG CCCTACAACA GCAGCAACAG CAACAGCAGC AGCAGCAACA GAGGCAGCCA 
GGAATGAAGC ACTCTCCCTC TCACCCTGCT GGACCCAAAG GTCACTTAGA TGCTGTTGGA 
CTCTCAGACA TGCAAGCCAA AGGGCACATG CCATATGGAT CTGGTTTTGG CTCAAGTGGG 
ATGGATTATG GTACAGTAGG TAAGGAGGCG GGAGCAGAGT CTCGATTTAA ACAGTGGACA 
TCCATGATGG AGGGGCTACC GTCCACTGTT TCTCAAGATA CCAGCCTGCA TAAAAATGGC 
TCCATTGCAC CCCCAGTAAA AAATCGTGGA GGTTCCCCCT ACCACCAATA TGACATGTTG 
TCTACGGATG CTTTGGGAGG GCATGGGGGG CCAACAGCTG AGAGCTGGTT ACCTGCCAAA 
TCACCCCCTT CAAGCAAAAT TGGAAACAAG TCAATCAATG CCAGCTGGCC CCCAGAGTTT 
CAGCCAGGAG TGCCATGGAA AGGGATCCAA AATATTGATC CAGAATCTGA TCCTTATGTT 
ACCCCTGGCA GTGTGCTTGG GGGTCCAGCC CCATCTCCCA TTGTAGATAC TGACCACCAG 
CTACTGCAGG ACACCCCCAC AGGGTCCAAT TCTTCCCTCA ACACCTCGCT GCCTTCACCT 
GGTGCCTGGC CCTTTAGTGC CTCTGAGAAT ATCCATAGCA CTTCAGCACT CTCTGCAAAG 
TTTGGGGACT ACAAATCGAC CTGGTCACCA GACCCCATAG GACATAACCC ATCACATCAC 
TCAAACAAGA TGTGGAAAAA CCAGATGTCC AGGAACAGCC CTGGGCTGCC TCGCCCACCT 
CCAGGGCTCA CAAACAACCC CAAAGCCACC AACTCTCCCT GGAACAGCAC AGCACCTCGC 
TCCGTCAGGG GCTGGGGCGG ACAGGACTCC AGAATGGCTT CTGCATCAGC TTGGAGTGAT 
GGTGGATCTG TGCGCCCCAG CTACTGGCTG ATCCTTCTTA ATCTCACACC ACAGATCGAT 
GGTTCCACCC TGCGAACGAT CTGCATGCAG CATGGCCCCC TGCTGACATT CCATCTCAAC 
CTTACCCAGG GCACAGCTCT AATCCGATAC AGCACCAAAC AGGAGGCAGC CAAGGCCCAG 
AGTGCCCTCC ATATGTGTGT TCTGGGTAAT ACAACTATCC TGGCGGAGTT TGCAACGGAT 
GAGGAGGTGA GCCGGTATCT TGCACAAGCT CAGCCACCAG CTCCCTCTGC TCCAGGAGCA 
GGCTGGCAGC CTTTGGAAGT GGGACCGAAC CACCCAGCTG AACCAGTAGG GCCAGCCCTA 
AACCTTTTTG GAGGAACCAC AGGGCTAGGG CAATGGAGCA GCGGCGGTGG TGGTAGTGAC 
CTGTCAGGGG TTTCTCTCTG GGGTACTCCT AGCAGCTACA CATCCAGTCT GTGGGGTGTG 
CCGAGCACAG AGGACGCCCA CAGGATGGGC AGCCCTGCCC CGTTACTACC CGGTGACCTT 
CTGGGAGGAG GGACCGACTC GATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816931.1
CDS	364..5970
Translation	MREKHLKKAVPREKEQEREEQLMEDKKRKKEDKKKKEAAQKVTEQKTKVPEVTKPSSSQATAASLPGSSPSPPVNGGNNAKRVAVPNGQPPSTARYMPREVPPRFRCQQDHKVLLKRGQPPPASCMLPGGGAGTPPSTTAPGAGPGNAQPGAGTLLPGESGASTDSSSGAAATSNYANSTWGSGGPTSSGPTTNPPHAWDKVIVDGSDMEEWPCIAGVDAEPPSDNATDNNSASNSASEKGPPQGSTTSHKGRGGQGQQQQQQQPGSECIQGVWKSDPKAKPVPSSNPASEGVNRLGNWRNGGGSERAGSASGFSNFNPNTNPSAWPALVQEGNRKGNPESDSSFSGAQLGPVGLTSREQQSKMENAGSSFAGSGRESATTHHTDGPKNGNTNSTNSNTLNPPDNKGTAFGVGSMDSCRGSDSPAQSTGERKNGGIGSWGAGRGQSATGDANCGHSNSGSNGREREDARKAPSSHRPNGSRGESWESKSGNGSWGYSPQADLSEVKWSEGNKLGAGGASQGEWKQPAGHEDLKVGEWGGLNQGNSSTGAWDNQKGHSLSDNQGNTSSAACWGRAPSSAGSEAGGQSTGSNPKAGGSGGSGDSLNSSSRRSHRPALPDSQAALQTLLNRTDLDPRVLSNSGWGQTQIKQDASWESEEVPSRTEGKSDRGTEGWENVSQAKTSGGWGDAPSQSNQNKSGWGEPPVPAPSTQEWKDTKATGWNDYKNSPTGWVGGGGGGVVGARPEDKAVPGWNNESGGAAEPGWSTRQSNPGGGWSAGKNGWGGNGSEDMEPPKAPGGWEGSGSKSSSGWGEVGQNDVGSWGNGANSNPAPRTGWEETKRPQGGGNWGEAARPQLPWNKQQESTPSSATPSTWGGSQAPPPPGNGRSQPTSGWKGGPQPTVSKEEEPSGWEEPSSQSIGHKMDIDDGTAAWGDPNNYNYKNVSLWDKNAQAGPGQMAREQGISGTLANKAAPSVWSKNKGPPTDNGTSAWGEPTEPASGWGEGEESGPPTPGWGSAPQVNPSAMKSGTKSMQDGWGESDGPVTGTRHSSWDEEEEGGVWNSAGSQGSGSSHNSAGWGQGGKKPQIKGPLKGGSNDTWMNTLPKQFSNMGLLSQVDDPSTTKMDHPGGLPDKKFETDKRGVNLGDFNDMMRKERSGFRLNNSKEMGAADSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSSLPSVGLGAIGSGLNPQTFAARQGGNHGLFGNSGAQSRGMHNPVPSLNSSPNLRAQVPPQFLSPQVSASMLKQFPNSNMNPGLFNMGPQLSPQQIAMLSQLPQIQQFQLACQLLLQQQQQQQQQQQQQQQLLQNQRKISQAVRQQQEQQQIARMVSALQQQQQQQQQQQQRQPGMKHSPSHPAGPKGHLDAVGLSDMQAKGHMPYGSGFGSSGMDYGTVGKEAGAESRFKQWTSMMEGLPSTVSQDTSLHKNGSIAPPVKNRGGSPYHQYDMLSTDALGGHGGPTAESWLPAKSPPSSKIGNKSINASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGPAPSPIVDTDHQLLQDTPTGSNSSLNTSLPSPGAWPFSASENIHSTSALSAKFGDYKSTWSPDPIGHNPSHHSNKMWKNQMSRNSPGLPRPPPGLTNNPKATNSPWNSTAPRSVRGWGGQDSRMASASAWSDGGSVRPSYWLILLNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQSALHMCVLGNTTILAEFATDEEVSRYLAQAQPPAPSAPGAGWQPLEVGPNHPAEPVGPALNLFGGTTGLGQWSSGGGGSDLSGVSLWGTPSSYTSSLWGVPSTEDAHRMGSPAPLLPGDLLGGGTDSI

Target ORF information:

RefSeq Version	XM_012961477.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961477.2

ORF Insert Sequence:

ATGAGGGAGA AACACTTGAA GAAAGCTGTT CCCAGAGAGA AGGAGCAAGA AAGGGAAGAA 
CAGTTAATGG AAGACAAGAA AAGGAAGAAA GAGGATAAAA AGAAGAAAGA AGCTGCTCAG 
AAGGTCACAG AACAAAAAAC CAAAGTGCCC GAAGTGACGA AACCAAGTTC AAGCCAAGCG 
ACGGCCGCCA GCCTACCTGG CAGCTCCCCC TCGCCACCAG TCAATGGTGG CAACAATGCC 
AAAAGGGTGG CAGTGCCGAA CGGACAACCG CCCAGCACCG CCCGCTATAT GCCTCGGGAG 
GTGCCGCCGC GATTCCGCTG CCAGCAGGAC CACAAAGTGT TACTGAAGCG TGGACAGCCT 
CCTCCAGCGT CCTGTATGCT GCCGGGGGGA GGAGCAGGGA CTCCCCCCTC AACCACTGCT 
CCAGGGGCCG GCCCAGGCAA CGCACAGCCA GGGGCGGGGA CACTGCTGCC AGGGGAAAGC 
GGGGCTTCAA CAGACTCATC ATCGGGGGCA GCTGCTACTT CAAATTATGC AAATTCCACT 
TGGGGCTCAG GAGGCCCCAC CAGCAGCGGC CCCACCACAA ACCCTCCCCA CGCCTGGGAC 
AAAGTGATTG TAGACGGGTC GGACATGGAA GAGTGGCCTT GCATAGCCGG CGTGGACGCA 
GAGCCCCCTT CCGACAACGC CACAGACAAC AACAGTGCCT CGAACTCAGC CTCGGAGAAG 
GGACCCCCAC AAGGAAGCAC CACTAGCCAC AAAGGGCGAG GGGGCCAGGG CCAGCAGCAA 
CAGCAGCAGC AGCCTGGTAG TGAATGTATC CAGGGCGTCT GGAAATCTGA CCCCAAGGCT 
AAGCCCGTCC CGTCTTCCAA CCCTGCTTCA GAGGGTGTAA ATAGACTGGG AAACTGGAGA 
AATGGGGGTG GGTCAGAGAG AGCAGGTTCT GCCTCTGGTT TCAGCAACTT TAATCCAAAT 
ACTAACCCAT CTGCTTGGCC TGCCCTTGTC CAGGAAGGCA ACAGGAAAGG GAACCCAGAG 
TCAGACAGCA GCTTTTCTGG CGCACAACTC GGCCCAGTGG GTTTGACCTC TCGGGAGCAG 
CAGTCAAAGA TGGAAAATGC AGGTTCCAGC TTTGCAGGAT CGGGCAGGGA GTCTGCCACA 
ACCCATCACA CTGACGGACC AAAAAATGGA AACACTAACT CTACGAACTC AAATACTTTG 
AACCCTCCTG ATAACAAGGG AACAGCTTTT GGGGTGGGCT CAATGGATTC CTGCCGGGGT 
TCAGACTCCC CTGCTCAAAG CACTGGAGAA AGAAAGAATG GGGGGATTGG CTCTTGGGGA 
GCGGGAAGGG GTCAGTCTGC CACAGGAGAT GCTAATTGTG GACATAGCAA CTCTGGGAGC 
AATGGACGAG AGAGAGAAGA CGCAAGGAAA GCGCCTTCAT CTCATAGACC CAATGGGTCA 
AGGGGGGAAT CTTGGGAAAG CAAAAGTGGG AATGGTTCTT GGGGTTACAG CCCGCAGGCA 
GACCTCAGTG AAGTGAAATG GAGTGAAGGG AACAAATTGG GGGCTGGAGG GGCGTCTCAG 
GGAGAATGGA AGCAGCCAGC CGGGCATGAG GACTTAAAGG TGGGAGAGTG GGGAGGCTTG 
AACCAAGGAA ACTCTAGCAC TGGAGCATGG GACAATCAGA AGGGCCACTC TCTATCGGAT 
AACCAGGGCA ACACGTCATC AGCAGCCTGC TGGGGCCGAG CCCCCAGCTC TGCAGGAAGT 
GAAGCTGGGG GGCAAAGCAC TGGAAGCAAC CCTAAAGCAG GGGGGAGCGG AGGAAGCGGA 
GACAGCCTGA ACTCCAGCAG TCGACGTTCA CACAGACCTG CCCTCCCAGA CAGTCAGGCT 
GCTCTTCAGA CTTTACTGAA TCGCACTGAT TTGGATCCCC GGGTGCTGTC CAACTCTGGC 
TGGGGCCAAA CCCAGATCAA GCAAGATGCA TCATGGGAGA GTGAGGAAGT GCCCTCTCGA 
ACTGAAGGCA AATCAGACCG TGGGACTGAA GGTTGGGAGA ACGTGTCCCA AGCCAAAACC 
TCAGGGGGTT GGGGGGATGC TCCCAGCCAA AGCAATCAAA ACAAGTCAGG CTGGGGGGAG 
CCCCCTGTCC CTGCACCTTC AACCCAAGAA TGGAAGGACA CCAAAGCAAC TGGCTGGAAT 
GACTATAAAA ACAGCCCAAC TGGTTGGGTT GGTGGAGGAG GTGGTGGGGT GGTTGGAGCA 
CGACCAGAGG ACAAGGCAGT TCCAGGTTGG AACAATGAAA GTGGTGGGGC TGCGGAGCCT 
GGGTGGAGTA CTCGCCAGTC TAATCCAGGA GGTGGCTGGT CAGCAGGAAA GAATGGTTGG 
GGGGGAAATG GTAGTGAGGA CATGGAGCCT CCCAAGGCCC CTGGAGGCTG GGAAGGCTCA 
GGAAGCAAAT CCTCCTCTGG CTGGGGTGAA GTTGGACAAA ATGATGTGGG TTCTTGGGGT 
AATGGGGCAA ACTCAAATCC AGCTCCACGA ACTGGCTGGG AGGAGACTAA GAGGCCCCAG 
GGAGGAGGAA ACTGGGGTGA GGCAGCTAGG CCCCAACTAC CCTGGAACAA ACAGCAGGAA 
TCAACGCCAT CTTCAGCAAC TCCTAGCACC TGGGGGGGCT CTCAGGCTCC CCCGCCACCT 
GGAAATGGGA GGTCACAGCC AACTTCAGGG TGGAAGGGTG GTCCCCAGCC AACTGTTTCC 
AAGGAGGAAG AGCCAAGTGG CTGGGAGGAA CCATCCTCAC AATCCATTGG TCATAAAATG 
GACATTGATG ACGGAACAGC AGCTTGGGGG GATCCTAACA ATTACAATTA CAAAAATGTC 
AGCCTGTGGG ACAAGAATGC TCAAGCAGGC CCTGGGCAGA TGGCTCGGGA GCAAGGAATA 
TCTGGGACAT TGGCAAACAA GGCAGCTCCC TCAGTGTGGA GTAAAAACAA AGGACCCCCA 
ACTGACAATG GGACATCAGC TTGGGGTGAG CCAACTGAAC CTGCTTCAGG GTGGGGAGAA 
GGTGAGGAGT CGGGTCCACC AACACCAGGA TGGGGAAGTG CACCCCAAGT AAATCCCAGT 
GCCATGAAAT CTGGTACAAA ATCTATGCAA GATGGCTGGG GTGAGAGTGA TGGGCCGGTG 
ACAGGAACAC GTCACAGCAG CTGGGATGAA GAAGAAGAGG GTGGAGTCTG GAACAGTGCA 
GGTTCTCAGG GAAGTGGCTC CTCCCACAAC TCTGCAGGTT GGGGACAAGG AGGAAAGAAA 
CCACAGATCA AGGGTCCCTT AAAAGGAGGG AGCAATGATA CCTGGATGAA CACCTTGCCC 
AAACAGTTCT CCAACATGGG ATTGTTGAGC CAAGTTGATG ACCCCTCTAC CACTAAGATG 
GACCATCCTG GCGGGCTTCC AGATAAGAAG TTTGAGACAG ACAAGCGGGG CGTGAATCTC 
GGGGATTTTA ATGACATGAT GCGGAAGGAA CGGTCTGGCT TCCGTCTGAA CAATTCCAAA 
GAGATGGGAG CTGCAGACAG TGGGCCTTAT TTTGAGAAGC TGACTTTGCC TTTCTCCAAT 
CAAGACGGGT GCCTTGGGGA TGAGGCTCCC TGTTCCCCTT TTTCTCCCTC CCCTAGCTAC 
AAGCTATCTC CCTCCGGCTC CTCGCTCCCT TCCGTGGGCC TGGGGGCTAT CGGTTCTGGC 
CTCAACCCCC AAACCTTCGC TGCTAGACAA GGTGGCAATC ACGGACTATT TGGAAACAGC 
GGTGCACAAT CGCGAGGCAT GCACAACCCT GTGCCATCGC TCAACTCCTC TCCCAACCTC 
CGGGCACAAG TGCCTCCCCA GTTCCTCTCT CCCCAGGTCT CAGCCTCCAT GCTAAAGCAG 
TTTCCTAACA GTAACATGAA TCCAGGACTT TTCAATATGG GGCCACAGCT TTCCCCTCAG 
CAGATTGCAA TGCTGAGCCA GCTTCCTCAA ATTCAGCAGT TCCAGCTGGC ATGTCAGCTC 
CTCTTGCAAC AACAACAACA GCAGCAGCAA CAGCAGCAGC AGCAGCAGCA ACTCCTTCAG 
AACCAGAGAA AGATCTCACA AGCGGTCAGA CAGCAGCAGG AACAGCAGCA GATAGCACGC 
ATGGTAAGTG CCCTACAACA GCAGCAACAG CAACAGCAGC AGCAGCAACA GAGGCAGCCA 
GGAATGAAGC ACTCTCCCTC TCACCCTGCT GGACCCAAAG GTCACTTAGA TGCTGTTGGA 
CTCTCAGACA TGCAAGCCAA AGGGCACATG CCATATGGAT CTGGTTTTGG CTCAAGTGGG 
ATGGATTATG GTACAGTAGG TAAGGAGGCG GGAGCAGAGT CTCGATTTAA ACAGTGGACA 
TCCATGATGG AGGGGCTACC GTCCACTGTT TCTCAAGATA CCAGCCTGCA TAAAAATGGC 
TCCATTGCAC CCCCAGTAAA AAATCGTGGA GGTTCCCCCT ACCACCAATA TGACATGTTG 
TCTACGGATG CTTTGGGAGG GCATGGGGGG CCAACAGCTG AGAGCTGGTT ACCTGCCAAA 
TCACCCCCTT CAAGCAAAAT TGGAAACAAG TCAATCAATG CCAGCTGGCC CCCAGAGTTT 
CAGCCAGGAG TGCCATGGAA AGGGATCCAA AATATTGATC CAGAATCTGA TCCTTATGTT 
ACCCCTGGCA GTGTGCTTGG GGGTCCAGCC CCATCTCCCA TTGTAGATAC TGACCACCAG 
CTACTGCAGG ACACCCCCAC AGGGTCCAAT TCTTCCCTCA ACACCTCGCT GCCTTCACCT 
GGTGCCTGGC CCTTTAGTGC CTCTGAGAAT ATCCATAGCA CTTCAGCACT CTCTGCAAAG 
TTTGGGGACT ACAAATCGAC CTGGTCACCA GACCCCATAG GACATAACCC ATCACATCAC 
TCAAACAAGA TGTGGAAAAA CCAGATGTCC AGGAACAGCC CTGGGCTGCC TCGCCCACCT 
CCAGGGCTCA CAAACAACCC CAAAGCCACC AACTCTCCCT GGAACAGCAC AGCACCTCGC 
TCCGTCAGGG GCTGGGGCGG ACAGGACTCC AGAATGGCTT CTGCATCAGC TTGGAGTGAT 
GGTGGATCTG TGCGCCCCAG CTACTGGCTG ATCCTTCTTA ATCTCACACC ACAGATCGAT 
GGTTCCACCC TGCGAACGAT CTGCATGCAG CATGGCCCCC TGCTGACATT CCATCTCAAC 
CTTACCCAGG GCACAGCTCT AATCCGATAC AGCACCAAAC AGGAGGCAGC CAAGGCCCAG 
AGTGCCCTCC ATATGTGTGT TCTGGGTAAT ACAACTATCC TGGCGGAGTT TGCAACGGAT 
GAGGAGGTGA GCCGGTATCT TGCACAAGCT CAGCCACCAG CTCCCTCTGC TCCAGGAGCA 
GGCTGGCAGC CTTTGGAAGT GGGACCGAAC CACCCAGCTG AACCAGTAGG GCCAGCCCTA 
AACCTTTTTG GAGGAACCAC AGGGCTAGGG CAATGGAGCA GCGGCGGTGG TGGTAGTGAC 
CTGTCAGGGG TTTCTCTCTG GGGTACTCCT AGCAGCTACA CATCCAGTCT GTGGGGTGTG 
CCGAGCACAG AGGACGCCCA CAGGATGGGC AGCCCTGCCC CGTTACTACC CGGTGACCTT 
CTGGGAGGAG GGACCGACTC GATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnrc6b ( XM_002934209.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934255.2 Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X5, mRNA.

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CloneID	OXa09307
Clone ID Related Accession (Same CDS sequence)	XM_012961478.2 , XM_012961477.2 , XM_002934209.4
Accession Version	XM_002934209.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trinucleotide repeat-containing gene 6B protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934209.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAGA AACACTTGAA GAAAGCTGTT CCCAGAGAGA AGGAGCAAGA AAGGGAAGAA 
CAGTTAATGG AAGACAAGAA AAGGAAGAAA GAGGATAAAA AGAAGAAAGA AGCTGCTCAG 
AAGGTCACAG AACAAAAAAC CAAAGTGCCC GAAGTGACGA AACCAAGTTC AAGCCAAGCG 
ACGGCCGCCA GCCTACCTGG CAGCTCCCCC TCGCCACCAG TCAATGGTGG CAACAATGCC 
AAAAGGGTGG CAGTGCCGAA CGGACAACCG CCCAGCACCG CCCGCTATAT GCCTCGGGAG 
GTGCCGCCGC GATTCCGCTG CCAGCAGGAC CACAAAGTGT TACTGAAGCG TGGACAGCCT 
CCTCCAGCGT CCTGTATGCT GCCGGGGGGA GGAGCAGGGA CTCCCCCCTC AACCACTGCT 
CCAGGGGCCG GCCCAGGCAA CGCACAGCCA GGGGCGGGGA CACTGCTGCC AGGGGAAAGC 
GGGGCTTCAA CAGACTCATC ATCGGGGGCA GCTGCTACTT CAAATTATGC AAATTCCACT 
TGGGGCTCAG GAGGCCCCAC CAGCAGCGGC CCCACCACAA ACCCTCCCCA CGCCTGGGAC 
AAAGTGATTG TAGACGGGTC GGACATGGAA GAGTGGCCTT GCATAGCCGG CGTGGACGCA 
GAGCCCCCTT CCGACAACGC CACAGACAAC AACAGTGCCT CGAACTCAGC CTCGGAGAAG 
GGACCCCCAC AAGGAAGCAC CACTAGCCAC AAAGGGCGAG GGGGCCAGGG CCAGCAGCAA 
CAGCAGCAGC AGCCTGGTAG TGAATGTATC CAGGGCGTCT GGAAATCTGA CCCCAAGGCT 
AAGCCCGTCC CGTCTTCCAA CCCTGCTTCA GAGGGTGTAA ATAGACTGGG AAACTGGAGA 
AATGGGGGTG GGTCAGAGAG AGCAGGTTCT GCCTCTGGTT TCAGCAACTT TAATCCAAAT 
ACTAACCCAT CTGCTTGGCC TGCCCTTGTC CAGGAAGGCA ACAGGAAAGG GAACCCAGAG 
TCAGACAGCA GCTTTTCTGG CGCACAACTC GGCCCAGTGG GTTTGACCTC TCGGGAGCAG 
CAGTCAAAGA TGGAAAATGC AGGTTCCAGC TTTGCAGGAT CGGGCAGGGA GTCTGCCACA 
ACCCATCACA CTGACGGACC AAAAAATGGA AACACTAACT CTACGAACTC AAATACTTTG 
AACCCTCCTG ATAACAAGGG AACAGCTTTT GGGGTGGGCT CAATGGATTC CTGCCGGGGT 
TCAGACTCCC CTGCTCAAAG CACTGGAGAA AGAAAGAATG GGGGGATTGG CTCTTGGGGA 
GCGGGAAGGG GTCAGTCTGC CACAGGAGAT GCTAATTGTG GACATAGCAA CTCTGGGAGC 
AATGGACGAG AGAGAGAAGA CGCAAGGAAA GCGCCTTCAT CTCATAGACC CAATGGGTCA 
AGGGGGGAAT CTTGGGAAAG CAAAAGTGGG AATGGTTCTT GGGGTTACAG CCCGCAGGCA 
GACCTCAGTG AAGTGAAATG GAGTGAAGGG AACAAATTGG GGGCTGGAGG GGCGTCTCAG 
GGAGAATGGA AGCAGCCAGC CGGGCATGAG GACTTAAAGG TGGGAGAGTG GGGAGGCTTG 
AACCAAGGAA ACTCTAGCAC TGGAGCATGG GACAATCAGA AGGGCCACTC TCTATCGGAT 
AACCAGGGCA ACACGTCATC AGCAGCCTGC TGGGGCCGAG CCCCCAGCTC TGCAGGAAGT 
GAAGCTGGGG GGCAAAGCAC TGGAAGCAAC CCTAAAGCAG GGGGGAGCGG AGGAAGCGGA 
GACAGCCTGA ACTCCAGCAG TCGACGTTCA CACAGACCTG CCCTCCCAGA CAGTCAGGCT 
GCTCTTCAGA CTTTACTGAA TCGCACTGAT TTGGATCCCC GGGTGCTGTC CAACTCTGGC 
TGGGGCCAAA CCCAGATCAA GCAAGATGCA TCATGGGAGA GTGAGGAAGT GCCCTCTCGA 
ACTGAAGGCA AATCAGACCG TGGGACTGAA GGTTGGGAGA ACGTGTCCCA AGCCAAAACC 
TCAGGGGGTT GGGGGGATGC TCCCAGCCAA AGCAATCAAA ACAAGTCAGG CTGGGGGGAG 
CCCCCTGTCC CTGCACCTTC AACCCAAGAA TGGAAGGACA CCAAAGCAAC TGGCTGGAAT 
GACTATAAAA ACAGCCCAAC TGGTTGGGTT GGTGGAGGAG GTGGTGGGGT GGTTGGAGCA 
CGACCAGAGG ACAAGGCAGT TCCAGGTTGG AACAATGAAA GTGGTGGGGC TGCGGAGCCT 
GGGTGGAGTA CTCGCCAGTC TAATCCAGGA GGTGGCTGGT CAGCAGGAAA GAATGGTTGG 
GGGGGAAATG GTAGTGAGGA CATGGAGCCT CCCAAGGCCC CTGGAGGCTG GGAAGGCTCA 
GGAAGCAAAT CCTCCTCTGG CTGGGGTGAA GTTGGACAAA ATGATGTGGG TTCTTGGGGT 
AATGGGGCAA ACTCAAATCC AGCTCCACGA ACTGGCTGGG AGGAGACTAA GAGGCCCCAG 
GGAGGAGGAA ACTGGGGTGA GGCAGCTAGG CCCCAACTAC CCTGGAACAA ACAGCAGGAA 
TCAACGCCAT CTTCAGCAAC TCCTAGCACC TGGGGGGGCT CTCAGGCTCC CCCGCCACCT 
GGAAATGGGA GGTCACAGCC AACTTCAGGG TGGAAGGGTG GTCCCCAGCC AACTGTTTCC 
AAGGAGGAAG AGCCAAGTGG CTGGGAGGAA CCATCCTCAC AATCCATTGG TCATAAAATG 
GACATTGATG ACGGAACAGC AGCTTGGGGG GATCCTAACA ATTACAATTA CAAAAATGTC 
AGCCTGTGGG ACAAGAATGC TCAAGCAGGC CCTGGGCAGA TGGCTCGGGA GCAAGGAATA 
TCTGGGACAT TGGCAAACAA GGCAGCTCCC TCAGTGTGGA GTAAAAACAA AGGACCCCCA 
ACTGACAATG GGACATCAGC TTGGGGTGAG CCAACTGAAC CTGCTTCAGG GTGGGGAGAA 
GGTGAGGAGT CGGGTCCACC AACACCAGGA TGGGGAAGTG CACCCCAAGT AAATCCCAGT 
GCCATGAAAT CTGGTACAAA ATCTATGCAA GATGGCTGGG GTGAGAGTGA TGGGCCGGTG 
ACAGGAACAC GTCACAGCAG CTGGGATGAA GAAGAAGAGG GTGGAGTCTG GAACAGTGCA 
GGTTCTCAGG GAAGTGGCTC CTCCCACAAC TCTGCAGGTT GGGGACAAGG AGGAAAGAAA 
CCACAGATCA AGGGTCCCTT AAAAGGAGGG AGCAATGATA CCTGGATGAA CACCTTGCCC 
AAACAGTTCT CCAACATGGG ATTGTTGAGC CAAGTTGATG ACCCCTCTAC CACTAAGATG 
GACCATCCTG GCGGGCTTCC AGATAAGAAG TTTGAGACAG ACAAGCGGGG CGTGAATCTC 
GGGGATTTTA ATGACATGAT GCGGAAGGAA CGGTCTGGCT TCCGTCTGAA CAATTCCAAA 
GAGATGGGAG CTGCAGACAG TGGGCCTTAT TTTGAGAAGC TGACTTTGCC TTTCTCCAAT 
CAAGACGGGT GCCTTGGGGA TGAGGCTCCC TGTTCCCCTT TTTCTCCCTC CCCTAGCTAC 
AAGCTATCTC CCTCCGGCTC CTCGCTCCCT TCCGTGGGCC TGGGGGCTAT CGGTTCTGGC 
CTCAACCCCC AAACCTTCGC TGCTAGACAA GGTGGCAATC ACGGACTATT TGGAAACAGC 
GGTGCACAAT CGCGAGGCAT GCACAACCCT GTGCCATCGC TCAACTCCTC TCCCAACCTC 
CGGGCACAAG TGCCTCCCCA GTTCCTCTCT CCCCAGGTCT CAGCCTCCAT GCTAAAGCAG 
TTTCCTAACA GTAACATGAA TCCAGGACTT TTCAATATGG GGCCACAGCT TTCCCCTCAG 
CAGATTGCAA TGCTGAGCCA GCTTCCTCAA ATTCAGCAGT TCCAGCTGGC ATGTCAGCTC 
CTCTTGCAAC AACAACAACA GCAGCAGCAA CAGCAGCAGC AGCAGCAGCA ACTCCTTCAG 
AACCAGAGAA AGATCTCACA AGCGGTCAGA CAGCAGCAGG AACAGCAGCA GATAGCACGC 
ATGGTAAGTG CCCTACAACA GCAGCAACAG CAACAGCAGC AGCAGCAACA GAGGCAGCCA 
GGAATGAAGC ACTCTCCCTC TCACCCTGCT GGACCCAAAG GTCACTTAGA TGCTGTTGGA 
CTCTCAGACA TGCAAGCCAA AGGGCACATG CCATATGGAT CTGGTTTTGG CTCAAGTGGG 
ATGGATTATG GTACAGTAGG TAAGGAGGCG GGAGCAGAGT CTCGATTTAA ACAGTGGACA 
TCCATGATGG AGGGGCTACC GTCCACTGTT TCTCAAGATA CCAGCCTGCA TAAAAATGGC 
TCCATTGCAC CCCCAGTAAA AAATCGTGGA GGTTCCCCCT ACCACCAATA TGACATGTTG 
TCTACGGATG CTTTGGGAGG GCATGGGGGG CCAACAGCTG AGAGCTGGTT ACCTGCCAAA 
TCACCCCCTT CAAGCAAAAT TGGAAACAAG TCAATCAATG CCAGCTGGCC CCCAGAGTTT 
CAGCCAGGAG TGCCATGGAA AGGGATCCAA AATATTGATC CAGAATCTGA TCCTTATGTT 
ACCCCTGGCA GTGTGCTTGG GGGTCCAGCC CCATCTCCCA TTGTAGATAC TGACCACCAG 
CTACTGCAGG ACACCCCCAC AGGGTCCAAT TCTTCCCTCA ACACCTCGCT GCCTTCACCT 
GGTGCCTGGC CCTTTAGTGC CTCTGAGAAT ATCCATAGCA CTTCAGCACT CTCTGCAAAG 
TTTGGGGACT ACAAATCGAC CTGGTCACCA GACCCCATAG GACATAACCC ATCACATCAC 
TCAAACAAGA TGTGGAAAAA CCAGATGTCC AGGAACAGCC CTGGGCTGCC TCGCCCACCT 
CCAGGGCTCA CAAACAACCC CAAAGCCACC AACTCTCCCT GGAACAGCAC AGCACCTCGC 
TCCGTCAGGG GCTGGGGCGG ACAGGACTCC AGAATGGCTT CTGCATCAGC TTGGAGTGAT 
GGTGGATCTG TGCGCCCCAG CTACTGGCTG ATCCTTCTTA ATCTCACACC ACAGATCGAT 
GGTTCCACCC TGCGAACGAT CTGCATGCAG CATGGCCCCC TGCTGACATT CCATCTCAAC 
CTTACCCAGG GCACAGCTCT AATCCGATAC AGCACCAAAC AGGAGGCAGC CAAGGCCCAG 
AGTGCCCTCC ATATGTGTGT TCTGGGTAAT ACAACTATCC TGGCGGAGTT TGCAACGGAT 
GAGGAGGTGA GCCGGTATCT TGCACAAGCT CAGCCACCAG CTCCCTCTGC TCCAGGAGCA 
GGCTGGCAGC CTTTGGAAGT GGGACCGAAC CACCCAGCTG AACCAGTAGG GCCAGCCCTA 
AACCTTTTTG GAGGAACCAC AGGGCTAGGG CAATGGAGCA GCGGCGGTGG TGGTAGTGAC 
CTGTCAGGGG TTTCTCTCTG GGGTACTCCT AGCAGCTACA CATCCAGTCT GTGGGGTGTG 
CCGAGCACAG AGGACGCCCA CAGGATGGGC AGCCCTGCCC CGTTACTACC CGGTGACCTT 
CTGGGAGGAG GGACCGACTC GATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934255.2
CDS	390..5996
Translation	MREKHLKKAVPREKEQEREEQLMEDKKRKKEDKKKKEAAQKVTEQKTKVPEVTKPSSSQATAASLPGSSPSPPVNGGNNAKRVAVPNGQPPSTARYMPREVPPRFRCQQDHKVLLKRGQPPPASCMLPGGGAGTPPSTTAPGAGPGNAQPGAGTLLPGESGASTDSSSGAAATSNYANSTWGSGGPTSSGPTTNPPHAWDKVIVDGSDMEEWPCIAGVDAEPPSDNATDNNSASNSASEKGPPQGSTTSHKGRGGQGQQQQQQQPGSECIQGVWKSDPKAKPVPSSNPASEGVNRLGNWRNGGGSERAGSASGFSNFNPNTNPSAWPALVQEGNRKGNPESDSSFSGAQLGPVGLTSREQQSKMENAGSSFAGSGRESATTHHTDGPKNGNTNSTNSNTLNPPDNKGTAFGVGSMDSCRGSDSPAQSTGERKNGGIGSWGAGRGQSATGDANCGHSNSGSNGREREDARKAPSSHRPNGSRGESWESKSGNGSWGYSPQADLSEVKWSEGNKLGAGGASQGEWKQPAGHEDLKVGEWGGLNQGNSSTGAWDNQKGHSLSDNQGNTSSAACWGRAPSSAGSEAGGQSTGSNPKAGGSGGSGDSLNSSSRRSHRPALPDSQAALQTLLNRTDLDPRVLSNSGWGQTQIKQDASWESEEVPSRTEGKSDRGTEGWENVSQAKTSGGWGDAPSQSNQNKSGWGEPPVPAPSTQEWKDTKATGWNDYKNSPTGWVGGGGGGVVGARPEDKAVPGWNNESGGAAEPGWSTRQSNPGGGWSAGKNGWGGNGSEDMEPPKAPGGWEGSGSKSSSGWGEVGQNDVGSWGNGANSNPAPRTGWEETKRPQGGGNWGEAARPQLPWNKQQESTPSSATPSTWGGSQAPPPPGNGRSQPTSGWKGGPQPTVSKEEEPSGWEEPSSQSIGHKMDIDDGTAAWGDPNNYNYKNVSLWDKNAQAGPGQMAREQGISGTLANKAAPSVWSKNKGPPTDNGTSAWGEPTEPASGWGEGEESGPPTPGWGSAPQVNPSAMKSGTKSMQDGWGESDGPVTGTRHSSWDEEEEGGVWNSAGSQGSGSSHNSAGWGQGGKKPQIKGPLKGGSNDTWMNTLPKQFSNMGLLSQVDDPSTTKMDHPGGLPDKKFETDKRGVNLGDFNDMMRKERSGFRLNNSKEMGAADSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSSLPSVGLGAIGSGLNPQTFAARQGGNHGLFGNSGAQSRGMHNPVPSLNSSPNLRAQVPPQFLSPQVSASMLKQFPNSNMNPGLFNMGPQLSPQQIAMLSQLPQIQQFQLACQLLLQQQQQQQQQQQQQQQLLQNQRKISQAVRQQQEQQQIARMVSALQQQQQQQQQQQQRQPGMKHSPSHPAGPKGHLDAVGLSDMQAKGHMPYGSGFGSSGMDYGTVGKEAGAESRFKQWTSMMEGLPSTVSQDTSLHKNGSIAPPVKNRGGSPYHQYDMLSTDALGGHGGPTAESWLPAKSPPSSKIGNKSINASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGPAPSPIVDTDHQLLQDTPTGSNSSLNTSLPSPGAWPFSASENIHSTSALSAKFGDYKSTWSPDPIGHNPSHHSNKMWKNQMSRNSPGLPRPPPGLTNNPKATNSPWNSTAPRSVRGWGGQDSRMASASAWSDGGSVRPSYWLILLNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQSALHMCVLGNTTILAEFATDEEVSRYLAQAQPPAPSAPGAGWQPLEVGPNHPAEPVGPALNLFGGTTGLGQWSSGGGGSDLSGVSLWGTPSSYTSSLWGVPSTEDAHRMGSPAPLLPGDLLGGGTDSI

Target ORF information:

RefSeq Version	XM_002934209.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934209.4

ORF Insert Sequence:

ATGAGGGAGA AACACTTGAA GAAAGCTGTT CCCAGAGAGA AGGAGCAAGA AAGGGAAGAA 
CAGTTAATGG AAGACAAGAA AAGGAAGAAA GAGGATAAAA AGAAGAAAGA AGCTGCTCAG 
AAGGTCACAG AACAAAAAAC CAAAGTGCCC GAAGTGACGA AACCAAGTTC AAGCCAAGCG 
ACGGCCGCCA GCCTACCTGG CAGCTCCCCC TCGCCACCAG TCAATGGTGG CAACAATGCC 
AAAAGGGTGG CAGTGCCGAA CGGACAACCG CCCAGCACCG CCCGCTATAT GCCTCGGGAG 
GTGCCGCCGC GATTCCGCTG CCAGCAGGAC CACAAAGTGT TACTGAAGCG TGGACAGCCT 
CCTCCAGCGT CCTGTATGCT GCCGGGGGGA GGAGCAGGGA CTCCCCCCTC AACCACTGCT 
CCAGGGGCCG GCCCAGGCAA CGCACAGCCA GGGGCGGGGA CACTGCTGCC AGGGGAAAGC 
GGGGCTTCAA CAGACTCATC ATCGGGGGCA GCTGCTACTT CAAATTATGC AAATTCCACT 
TGGGGCTCAG GAGGCCCCAC CAGCAGCGGC CCCACCACAA ACCCTCCCCA CGCCTGGGAC 
AAAGTGATTG TAGACGGGTC GGACATGGAA GAGTGGCCTT GCATAGCCGG CGTGGACGCA 
GAGCCCCCTT CCGACAACGC CACAGACAAC AACAGTGCCT CGAACTCAGC CTCGGAGAAG 
GGACCCCCAC AAGGAAGCAC CACTAGCCAC AAAGGGCGAG GGGGCCAGGG CCAGCAGCAA 
CAGCAGCAGC AGCCTGGTAG TGAATGTATC CAGGGCGTCT GGAAATCTGA CCCCAAGGCT 
AAGCCCGTCC CGTCTTCCAA CCCTGCTTCA GAGGGTGTAA ATAGACTGGG AAACTGGAGA 
AATGGGGGTG GGTCAGAGAG AGCAGGTTCT GCCTCTGGTT TCAGCAACTT TAATCCAAAT 
ACTAACCCAT CTGCTTGGCC TGCCCTTGTC CAGGAAGGCA ACAGGAAAGG GAACCCAGAG 
TCAGACAGCA GCTTTTCTGG CGCACAACTC GGCCCAGTGG GTTTGACCTC TCGGGAGCAG 
CAGTCAAAGA TGGAAAATGC AGGTTCCAGC TTTGCAGGAT CGGGCAGGGA GTCTGCCACA 
ACCCATCACA CTGACGGACC AAAAAATGGA AACACTAACT CTACGAACTC AAATACTTTG 
AACCCTCCTG ATAACAAGGG AACAGCTTTT GGGGTGGGCT CAATGGATTC CTGCCGGGGT 
TCAGACTCCC CTGCTCAAAG CACTGGAGAA AGAAAGAATG GGGGGATTGG CTCTTGGGGA 
GCGGGAAGGG GTCAGTCTGC CACAGGAGAT GCTAATTGTG GACATAGCAA CTCTGGGAGC 
AATGGACGAG AGAGAGAAGA CGCAAGGAAA GCGCCTTCAT CTCATAGACC CAATGGGTCA 
AGGGGGGAAT CTTGGGAAAG CAAAAGTGGG AATGGTTCTT GGGGTTACAG CCCGCAGGCA 
GACCTCAGTG AAGTGAAATG GAGTGAAGGG AACAAATTGG GGGCTGGAGG GGCGTCTCAG 
GGAGAATGGA AGCAGCCAGC CGGGCATGAG GACTTAAAGG TGGGAGAGTG GGGAGGCTTG 
AACCAAGGAA ACTCTAGCAC TGGAGCATGG GACAATCAGA AGGGCCACTC TCTATCGGAT 
AACCAGGGCA ACACGTCATC AGCAGCCTGC TGGGGCCGAG CCCCCAGCTC TGCAGGAAGT 
GAAGCTGGGG GGCAAAGCAC TGGAAGCAAC CCTAAAGCAG GGGGGAGCGG AGGAAGCGGA 
GACAGCCTGA ACTCCAGCAG TCGACGTTCA CACAGACCTG CCCTCCCAGA CAGTCAGGCT 
GCTCTTCAGA CTTTACTGAA TCGCACTGAT TTGGATCCCC GGGTGCTGTC CAACTCTGGC 
TGGGGCCAAA CCCAGATCAA GCAAGATGCA TCATGGGAGA GTGAGGAAGT GCCCTCTCGA 
ACTGAAGGCA AATCAGACCG TGGGACTGAA GGTTGGGAGA ACGTGTCCCA AGCCAAAACC 
TCAGGGGGTT GGGGGGATGC TCCCAGCCAA AGCAATCAAA ACAAGTCAGG CTGGGGGGAG 
CCCCCTGTCC CTGCACCTTC AACCCAAGAA TGGAAGGACA CCAAAGCAAC TGGCTGGAAT 
GACTATAAAA ACAGCCCAAC TGGTTGGGTT GGTGGAGGAG GTGGTGGGGT GGTTGGAGCA 
CGACCAGAGG ACAAGGCAGT TCCAGGTTGG AACAATGAAA GTGGTGGGGC TGCGGAGCCT 
GGGTGGAGTA CTCGCCAGTC TAATCCAGGA GGTGGCTGGT CAGCAGGAAA GAATGGTTGG 
GGGGGAAATG GTAGTGAGGA CATGGAGCCT CCCAAGGCCC CTGGAGGCTG GGAAGGCTCA 
GGAAGCAAAT CCTCCTCTGG CTGGGGTGAA GTTGGACAAA ATGATGTGGG TTCTTGGGGT 
AATGGGGCAA ACTCAAATCC AGCTCCACGA ACTGGCTGGG AGGAGACTAA GAGGCCCCAG 
GGAGGAGGAA ACTGGGGTGA GGCAGCTAGG CCCCAACTAC CCTGGAACAA ACAGCAGGAA 
TCAACGCCAT CTTCAGCAAC TCCTAGCACC TGGGGGGGCT CTCAGGCTCC CCCGCCACCT 
GGAAATGGGA GGTCACAGCC AACTTCAGGG TGGAAGGGTG GTCCCCAGCC AACTGTTTCC 
AAGGAGGAAG AGCCAAGTGG CTGGGAGGAA CCATCCTCAC AATCCATTGG TCATAAAATG 
GACATTGATG ACGGAACAGC AGCTTGGGGG GATCCTAACA ATTACAATTA CAAAAATGTC 
AGCCTGTGGG ACAAGAATGC TCAAGCAGGC CCTGGGCAGA TGGCTCGGGA GCAAGGAATA 
TCTGGGACAT TGGCAAACAA GGCAGCTCCC TCAGTGTGGA GTAAAAACAA AGGACCCCCA 
ACTGACAATG GGACATCAGC TTGGGGTGAG CCAACTGAAC CTGCTTCAGG GTGGGGAGAA 
GGTGAGGAGT CGGGTCCACC AACACCAGGA TGGGGAAGTG CACCCCAAGT AAATCCCAGT 
GCCATGAAAT CTGGTACAAA ATCTATGCAA GATGGCTGGG GTGAGAGTGA TGGGCCGGTG 
ACAGGAACAC GTCACAGCAG CTGGGATGAA GAAGAAGAGG GTGGAGTCTG GAACAGTGCA 
GGTTCTCAGG GAAGTGGCTC CTCCCACAAC TCTGCAGGTT GGGGACAAGG AGGAAAGAAA 
CCACAGATCA AGGGTCCCTT AAAAGGAGGG AGCAATGATA CCTGGATGAA CACCTTGCCC 
AAACAGTTCT CCAACATGGG ATTGTTGAGC CAAGTTGATG ACCCCTCTAC CACTAAGATG 
GACCATCCTG GCGGGCTTCC AGATAAGAAG TTTGAGACAG ACAAGCGGGG CGTGAATCTC 
GGGGATTTTA ATGACATGAT GCGGAAGGAA CGGTCTGGCT TCCGTCTGAA CAATTCCAAA 
GAGATGGGAG CTGCAGACAG TGGGCCTTAT TTTGAGAAGC TGACTTTGCC TTTCTCCAAT 
CAAGACGGGT GCCTTGGGGA TGAGGCTCCC TGTTCCCCTT TTTCTCCCTC CCCTAGCTAC 
AAGCTATCTC CCTCCGGCTC CTCGCTCCCT TCCGTGGGCC TGGGGGCTAT CGGTTCTGGC 
CTCAACCCCC AAACCTTCGC TGCTAGACAA GGTGGCAATC ACGGACTATT TGGAAACAGC 
GGTGCACAAT CGCGAGGCAT GCACAACCCT GTGCCATCGC TCAACTCCTC TCCCAACCTC 
CGGGCACAAG TGCCTCCCCA GTTCCTCTCT CCCCAGGTCT CAGCCTCCAT GCTAAAGCAG 
TTTCCTAACA GTAACATGAA TCCAGGACTT TTCAATATGG GGCCACAGCT TTCCCCTCAG 
CAGATTGCAA TGCTGAGCCA GCTTCCTCAA ATTCAGCAGT TCCAGCTGGC ATGTCAGCTC 
CTCTTGCAAC AACAACAACA GCAGCAGCAA CAGCAGCAGC AGCAGCAGCA ACTCCTTCAG 
AACCAGAGAA AGATCTCACA AGCGGTCAGA CAGCAGCAGG AACAGCAGCA GATAGCACGC 
ATGGTAAGTG CCCTACAACA GCAGCAACAG CAACAGCAGC AGCAGCAACA GAGGCAGCCA 
GGAATGAAGC ACTCTCCCTC TCACCCTGCT GGACCCAAAG GTCACTTAGA TGCTGTTGGA 
CTCTCAGACA TGCAAGCCAA AGGGCACATG CCATATGGAT CTGGTTTTGG CTCAAGTGGG 
ATGGATTATG GTACAGTAGG TAAGGAGGCG GGAGCAGAGT CTCGATTTAA ACAGTGGACA 
TCCATGATGG AGGGGCTACC GTCCACTGTT TCTCAAGATA CCAGCCTGCA TAAAAATGGC 
TCCATTGCAC CCCCAGTAAA AAATCGTGGA GGTTCCCCCT ACCACCAATA TGACATGTTG 
TCTACGGATG CTTTGGGAGG GCATGGGGGG CCAACAGCTG AGAGCTGGTT ACCTGCCAAA 
TCACCCCCTT CAAGCAAAAT TGGAAACAAG TCAATCAATG CCAGCTGGCC CCCAGAGTTT 
CAGCCAGGAG TGCCATGGAA AGGGATCCAA AATATTGATC CAGAATCTGA TCCTTATGTT 
ACCCCTGGCA GTGTGCTTGG GGGTCCAGCC CCATCTCCCA TTGTAGATAC TGACCACCAG 
CTACTGCAGG ACACCCCCAC AGGGTCCAAT TCTTCCCTCA ACACCTCGCT GCCTTCACCT 
GGTGCCTGGC CCTTTAGTGC CTCTGAGAAT ATCCATAGCA CTTCAGCACT CTCTGCAAAG 
TTTGGGGACT ACAAATCGAC CTGGTCACCA GACCCCATAG GACATAACCC ATCACATCAC 
TCAAACAAGA TGTGGAAAAA CCAGATGTCC AGGAACAGCC CTGGGCTGCC TCGCCCACCT 
CCAGGGCTCA CAAACAACCC CAAAGCCACC AACTCTCCCT GGAACAGCAC AGCACCTCGC 
TCCGTCAGGG GCTGGGGCGG ACAGGACTCC AGAATGGCTT CTGCATCAGC TTGGAGTGAT 
GGTGGATCTG TGCGCCCCAG CTACTGGCTG ATCCTTCTTA ATCTCACACC ACAGATCGAT 
GGTTCCACCC TGCGAACGAT CTGCATGCAG CATGGCCCCC TGCTGACATT CCATCTCAAC 
CTTACCCAGG GCACAGCTCT AATCCGATAC AGCACCAAAC AGGAGGCAGC CAAGGCCCAG 
AGTGCCCTCC ATATGTGTGT TCTGGGTAAT ACAACTATCC TGGCGGAGTT TGCAACGGAT 
GAGGAGGTGA GCCGGTATCT TGCACAAGCT CAGCCACCAG CTCCCTCTGC TCCAGGAGCA 
GGCTGGCAGC CTTTGGAAGT GGGACCGAAC CACCCAGCTG AACCAGTAGG GCCAGCCCTA 
AACCTTTTTG GAGGAACCAC AGGGCTAGGG CAATGGAGCA GCGGCGGTGG TGGTAGTGAC 
CTGTCAGGGG TTTCTCTCTG GGGTACTCCT AGCAGCTACA CATCCAGTCT GTGGGGTGTG 
CCGAGCACAG AGGACGCCCA CAGGATGGGC AGCCCTGCCC CGTTACTACC CGGTGACCTT 
CTGGGAGGAG GGACCGACTC GATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnrc6b ( XM_012961481.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816935.1 Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X6, mRNA.

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CloneID	OXa37107
Clone ID Related Accession (Same CDS sequence)	XM_012961481.2
Accession Version	XM_012961481.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trinucleotide repeat-containing gene 6B protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961481.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA AGAAAAGGAA GAAAGAGGAT AAAAAGAAGA AAGAAGCTGC TCAGAAGGTC 
ACAGAACAAA AAACCAAAGT GCCCGAAGTG ACGAAACCAA GTTCAAGCCA AGCGACGGCC 
GCCAGCCTAC CTGGCAGCTC CCCCTCGCCA CCAGTCAATG GTGGCAACAA TGCCAAAAGG 
GTGGCAGTGC CGAACGGACA ACCGCCCAGC ACCGCCCGCT ATATGCCTCG GGAGGTGCCG 
CCGCGATTCC GCTGCCAGCA GGACCACAAA GTGTTACTGA AGCGTGGACA GCCTCCTCCA 
GCGTCCTGTA TGCTGCCGGG GGGAGGAGCA GGGACTCCCC CCTCAACCAC TGCTCCAGGG 
GCCGGCCCAG GCAACGCACA GCCAGGGGCG GGGACACTGC TGCCAGGGGA AAGCGGGGCT 
TCAACAGACT CATCATCGGG GGCAGCTGCT ACTTCAAATT ATGCAAATTC CACTTGGGGC 
TCAGGAGGCC CCACCAGCAG CGGCCCCACC ACAAACCCTC CCCACGCCTG GGACAAAGTG 
ATTGTAGACG GGTCGGACAT GGAAGAGTGG CCTTGCATAG CCGGCGTGGA CGCAGAGCCC 
CCTTCCGACA ACGCCACAGA CAACAACAGT GCCTCGAACT CAGCCTCGGA GAAGGGACCC 
CCACAAGGAA GCACCACTAG CCACAAAGGG CGAGGGGGCC AGGGCCAGCA GCAACAGCAG 
CAGCAGCCTG GTAGTGAATG TATCCAGGGC GTCTGGAAAT CTGACCCCAA GGCTAAGCCC 
GTCCCGTCTT CCAACCCTGC TTCAGAGGGT GTAAATAGAC TGGGAAACTG GAGAAATGGG 
GGTGGGTCAG AGAGAGCAGG TTCTGCCTCT GGTTTCAGCA ACTTTAATCC AAATACTAAC 
CCATCTGCTT GGCCTGCCCT TGTCCAGGAA GGCAACAGGA AAGGGAACCC AGAGTCAGAC 
AGCAGCTTTT CTGGCGCACA ACTCGGCCCA GTGGGTTTGA CCTCTCGGGA GCAGCAGTCA 
AAGATGGAAA ATGCAGGTTC CAGCTTTGCA GGATCGGGCA GGGAGTCTGC CACAACCCAT 
CACACTGACG GACCAAAAAA TGGAAACACT AACTCTACGA ACTCAAATAC TTTGAACCCT 
CCTGATAACA AGGGAACAGC TTTTGGGGTG GGCTCAATGG ATTCCTGCCG GGGTTCAGAC 
TCCCCTGCTC AAAGCACTGG AGAAAGAAAG AATGGGGGGA TTGGCTCTTG GGGAGCGGGA 
AGGGGTCAGT CTGCCACAGG AGATGCTAAT TGTGGACATA GCAACTCTGG GAGCAATGGA 
CGAGAGAGAG AAGACGCAAG GAAAGCGCCT TCATCTCATA GACCCAATGG GTCAAGGGGG 
GAATCTTGGG AAAGCAAAAG TGGGAATGGT TCTTGGGGTT ACAGCCCGCA GGCAGACCTC 
AGTGAAGTGA AATGGAGTGA AGGGAACAAA TTGGGGGCTG GAGGGGCGTC TCAGGGAGAA 
TGGAAGCAGC CAGCCGGGCA TGAGGACTTA AAGGTGGGAG AGTGGGGAGG CTTGAACCAA 
GGAAACTCTA GCACTGGAGC ATGGGACAAT CAGAAGGGCC ACTCTCTATC GGATAACCAG 
GGCAACACGT CATCAGCAGC CTGCTGGGGC CGAGCCCCCA GCTCTGCAGG AAGTGAAGCT 
GGGGGGCAAA GCACTGGAAG CAACCCTAAA GCAGGGGGGA GCGGAGGAAG CGGAGACAGC 
CTGAACTCCA GCAGTCGACG TTCACACAGA CCTGCCCTCC CAGACAGTCA GGCTGCTCTT 
CAGACTTTAC TGAATCGCAC TGATTTGGAT CCCCGGGTGC TGTCCAACTC TGGCTGGGGC 
CAAACCCAGA TCAAGCAAGA TGCATCATGG GAGAGTGAGG AAGTGCCCTC TCGAACTGAA 
GGCAAATCAG ACCGTGGGAC TGAAGGTTGG GAGAACGTGT CCCAAGCCAA AACCTCAGGG 
GGTTGGGGGG ATGCTCCCAG CCAAAGCAAT CAAAACAAGT CAGGCTGGGG GGAGCCCCCT 
GTCCCTGCAC CTTCAACCCA AGAATGGAAG GACACCAAAG CAACTGGCTG GAATGACTAT 
AAAAACAGCC CAACTGGTTG GGTTGGTGGA GGAGGTGGTG GGGTGGTTGG AGCACGACCA 
GAGGACAAGG CAGTTCCAGG TTGGAACAAT GAAAGTGGTG GGGCTGCGGA GCCTGGGTGG 
AGTACTCGCC AGTCTAATCC AGGAGGTGGC TGGTCAGCAG GAAAGAATGG TTGGGGGGGA 
AATGGTAGTG AGGACATGGA GCCTCCCAAG GCCCCTGGAG GCTGGGAAGG CTCAGGAAGC 
AAATCCTCCT CTGGCTGGGG TGAAGTTGGA CAAAATGATG TGGGTTCTTG GGGTAATGGG 
GCAAACTCAA ATCCAGCTCC ACGAACTGGC TGGGAGGAGA CTAAGAGGCC CCAGGGAGGA 
GGAAACTGGG GTGAGGCAGC TAGGCCCCAA CTACCCTGGA ACAAACAGCA GGAATCAACG 
CCATCTTCAG CAACTCCTAG CACCTGGGGG GGCTCTCAGG CTCCCCCGCC ACCTGGAAAT 
GGGAGGTCAC AGCCAACTTC AGGGTGGAAG GGTGGTCCCC AGCCAACTGT TTCCAAGGAG 
GAAGAGCCAA GTGGCTGGGA GGAACCATCC TCACAATCCA TTGGTCATAA AATGGACATT 
GATGACGGAA CAGCAGCTTG GGGGGATCCT AACAATTACA ATTACAAAAA TGTCAGCCTG 
TGGGACAAGA ATGCTCAAGC AGGCCCTGGG CAGATGGCTC GGGAGCAAGG AATATCTGGG 
ACATTGGCAA ACAAGGCAGC TCCCTCAGTG TGGAGTAAAA ACAAAGGACC CCCAACTGAC 
AATGGGACAT CAGCTTGGGG TGAGCCAACT GAACCTGCTT CAGGGTGGGG AGAAGGTGAG 
GAGTCGGGTC CACCAACACC AGGATGGGGA AGTGCACCCC AAGTAAATCC CAGTGCCATG 
AAATCTGGTA CAAAATCTAT GCAAGATGGC TGGGGTGAGA GTGATGGGCC GGTGACAGGA 
ACACGTCACA GCAGCTGGGA TGAAGAAGAA GAGGGTGGAG TCTGGAACAG TGCAGGTTCT 
CAGGGAAGTG GCTCCTCCCA CAACTCTGCA GGTTGGGGAC AAGGAGGAAA GAAACCACAG 
ATCAAGGGTC CCTTAAAAGG AGGGAGCAAT GATACCTGGA TGAACACCTT GCCCAAACAG 
TTCTCCAACA TGGGATTGTT GAGCCAAGTT GATGACCCCT CTACCACTAA GATGGACCAT 
CCTGGCGGGC TTCCAGATAA GAAGTTTGAG ACAGACAAGC GGGGCGTGAA TCTCGGGGAT 
TTTAATGACA TGATGCGGAA GGAACGGTCT GGCTTCCGTC TGAACAATTC CAAAGAGATG 
GGAGCTGCAG ACAGTGGGCC TTATTTTGAG AAGCTGACTT TGCCTTTCTC CAATCAAGAC 
GGGTGCCTTG GGGATGAGGC TCCCTGTTCC CCTTTTTCTC CCTCCCCTAG CTACAAGCTA 
TCTCCCTCCG GCTCCTCGCT CCCTTCCGTG GGCCTGGGGG CTATCGGTTC TGGCCTCAAC 
CCCCAAACCT TCGCTGCTAG ACAAGGTGGC AATCACGGAC TATTTGGAAA CAGCGGTGCA 
CAATCGCGAG GCATGCACAA CCCTGTGCCA TCGCTCAACT CCTCTCCCAA CCTCCGGGCA 
CAAGTGCCTC CCCAGTTCCT CTCTCCCCAG GTCTCAGCCT CCATGCTAAA GCAGTTTCCT 
AACAGTAACA TGAATCCAGG ACTTTTCAAT ATGGGGCCAC AGCTTTCCCC TCAGCAGATT 
GCAATGCTGA GCCAGCTTCC TCAAATTCAG CAGTTCCAGC TGGCATGTCA GCTCCTCTTG 
CAACAACAAC AACAGCAGCA GCAACAGCAG CAGCAGCAGC AGCAACTCCT TCAGAACCAG 
AGAAAGATCT CACAAGCGGT CAGACAGCAG CAGGAACAGC AGCAGATAGC ACGCATGGTA 
AGTGCCCTAC AACAGCAGCA ACAGCAACAG CAGCAGCAGC AACAGAGGCA GCCAGGAATG 
AAGCACTCTC CCTCTCACCC TGCTGGACCC AAAGGTCACT TAGATGCTGT TGGACTCTCA 
GACATGCAAG CCAAAGGGCA CATGCCATAT GGATCTGGTT TTGGCTCAAG TGGGATGGAT 
TATGGTACAG TAGGTAAGGA GGCGGGAGCA GAGTCTCGAT TTAAACAGTG GACATCCATG 
ATGGAGGGGC TACCGTCCAC TGTTTCTCAA GATACCAGCC TGCATAAAAA TGGCTCCATT 
GCACCCCCAG TAAAAAATCG TGGAGGTTCC CCCTACCACC AATATGACAT GTTGTCTACG 
GATGCTTTGG GAGGGCATGG GGGGCCAACA GCTGAGAGCT GGTTACCTGC CAAATCACCC 
CCTTCAAGCA AAATTGGAAA CAAGTCAATC AATGCCAGCT GGCCCCCAGA GTTTCAGCCA 
GGAGTGCCAT GGAAAGGGAT CCAAAATATT GATCCAGAAT CTGATCCTTA TGTTACCCCT 
GGCAGTGTGC TTGGGGGTCC AGCCCCATCT CCCATTGTAG ATACTGACCA CCAGCTACTG 
CAGGACACCC CCACAGGGTC CAATTCTTCC CTCAACACCT CGCTGCCTTC ACCTGGTGCC 
TGGCCCTTTA GTGCCTCTGA GAATATCCAT AGCACTTCAG CACTCTCTGC AAAGTTTGGG 
GACTACAAAT CGACCTGGTC ACCAGACCCC ATAGGACATA ACCCATCACA TCACTCAAAC 
AAGATGTGGA AAAACCAGAT GTCCAGGAAC AGCCCTGGGC TGCCTCGCCC ACCTCCAGGG 
CTCACAAACA ACCCCAAAGC CACCAACTCT CCCTGGAACA GCACAGCACC TCGCTCCGTC 
AGGGGCTGGG GCGGACAGGA CTCCAGAATG GCTTCTGCAT CAGCTTGGAG TGATGGTGGA 
TCTGTGCGCC CCAGCTACTG GCTGATCCTT CTTAATCTCA CACCACAGAT CGATGGTTCC 
ACCCTGCGAA CGATCTGCAT GCAGCATGGC CCCCTGCTGA CATTCCATCT CAACCTTACC 
CAGGGCACAG CTCTAATCCG ATACAGCACC AAACAGGAGG CAGCCAAGGC CCAGAGTGCC 
CTCCATATGT GTGTTCTGGG TAATACAACT ATCCTGGCGG AGTTTGCAAC GGATGAGGAG 
GTGAGCCGGT ATCTTGCACA AGCTCAGCCA CCAGCTCCCT CTGCTCCAGG AGCAGGCTGG 
CAGCCTTTGG AAGTGGGACC GAACCACCCA GCTGAACCAG TAGGGCCAGC CCTAAACCTT 
TTTGGAGGAA CCACAGGGCT AGGGCAATGG AGCAGCGGCG GTGGTGGTAG TGACCTGTCA 
GGGGTTTCTC TCTGGGGTAC TCCTAGCAGC TACACATCCA GTCTGTGGGG TGTGCCGAGC 
ACAGAGGACG CCCACAGGAT GGGCAGCCCT GCCCCGTTAC TACCCGGTGA CCTTCTGGGA 
GGAGGGACCG ACTCGATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816935.1
CDS	335..5875
Translation	MEDKKRKKEDKKKKEAAQKVTEQKTKVPEVTKPSSSQATAASLPGSSPSPPVNGGNNAKRVAVPNGQPPSTARYMPREVPPRFRCQQDHKVLLKRGQPPPASCMLPGGGAGTPPSTTAPGAGPGNAQPGAGTLLPGESGASTDSSSGAAATSNYANSTWGSGGPTSSGPTTNPPHAWDKVIVDGSDMEEWPCIAGVDAEPPSDNATDNNSASNSASEKGPPQGSTTSHKGRGGQGQQQQQQQPGSECIQGVWKSDPKAKPVPSSNPASEGVNRLGNWRNGGGSERAGSASGFSNFNPNTNPSAWPALVQEGNRKGNPESDSSFSGAQLGPVGLTSREQQSKMENAGSSFAGSGRESATTHHTDGPKNGNTNSTNSNTLNPPDNKGTAFGVGSMDSCRGSDSPAQSTGERKNGGIGSWGAGRGQSATGDANCGHSNSGSNGREREDARKAPSSHRPNGSRGESWESKSGNGSWGYSPQADLSEVKWSEGNKLGAGGASQGEWKQPAGHEDLKVGEWGGLNQGNSSTGAWDNQKGHSLSDNQGNTSSAACWGRAPSSAGSEAGGQSTGSNPKAGGSGGSGDSLNSSSRRSHRPALPDSQAALQTLLNRTDLDPRVLSNSGWGQTQIKQDASWESEEVPSRTEGKSDRGTEGWENVSQAKTSGGWGDAPSQSNQNKSGWGEPPVPAPSTQEWKDTKATGWNDYKNSPTGWVGGGGGGVVGARPEDKAVPGWNNESGGAAEPGWSTRQSNPGGGWSAGKNGWGGNGSEDMEPPKAPGGWEGSGSKSSSGWGEVGQNDVGSWGNGANSNPAPRTGWEETKRPQGGGNWGEAARPQLPWNKQQESTPSSATPSTWGGSQAPPPPGNGRSQPTSGWKGGPQPTVSKEEEPSGWEEPSSQSIGHKMDIDDGTAAWGDPNNYNYKNVSLWDKNAQAGPGQMAREQGISGTLANKAAPSVWSKNKGPPTDNGTSAWGEPTEPASGWGEGEESGPPTPGWGSAPQVNPSAMKSGTKSMQDGWGESDGPVTGTRHSSWDEEEEGGVWNSAGSQGSGSSHNSAGWGQGGKKPQIKGPLKGGSNDTWMNTLPKQFSNMGLLSQVDDPSTTKMDHPGGLPDKKFETDKRGVNLGDFNDMMRKERSGFRLNNSKEMGAADSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSSLPSVGLGAIGSGLNPQTFAARQGGNHGLFGNSGAQSRGMHNPVPSLNSSPNLRAQVPPQFLSPQVSASMLKQFPNSNMNPGLFNMGPQLSPQQIAMLSQLPQIQQFQLACQLLLQQQQQQQQQQQQQQQLLQNQRKISQAVRQQQEQQQIARMVSALQQQQQQQQQQQQRQPGMKHSPSHPAGPKGHLDAVGLSDMQAKGHMPYGSGFGSSGMDYGTVGKEAGAESRFKQWTSMMEGLPSTVSQDTSLHKNGSIAPPVKNRGGSPYHQYDMLSTDALGGHGGPTAESWLPAKSPPSSKIGNKSINASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGPAPSPIVDTDHQLLQDTPTGSNSSLNTSLPSPGAWPFSASENIHSTSALSAKFGDYKSTWSPDPIGHNPSHHSNKMWKNQMSRNSPGLPRPPPGLTNNPKATNSPWNSTAPRSVRGWGGQDSRMASASAWSDGGSVRPSYWLILLNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQSALHMCVLGNTTILAEFATDEEVSRYLAQAQPPAPSAPGAGWQPLEVGPNHPAEPVGPALNLFGGTTGLGQWSSGGGGSDLSGVSLWGTPSSYTSSLWGVPSTEDAHRMGSPAPLLPGDLLGGGTDSI

Target ORF information:

RefSeq Version	XM_012961481.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961481.2

ORF Insert Sequence:

ATGGAAGACA AGAAAAGGAA GAAAGAGGAT AAAAAGAAGA AAGAAGCTGC TCAGAAGGTC 
ACAGAACAAA AAACCAAAGT GCCCGAAGTG ACGAAACCAA GTTCAAGCCA AGCGACGGCC 
GCCAGCCTAC CTGGCAGCTC CCCCTCGCCA CCAGTCAATG GTGGCAACAA TGCCAAAAGG 
GTGGCAGTGC CGAACGGACA ACCGCCCAGC ACCGCCCGCT ATATGCCTCG GGAGGTGCCG 
CCGCGATTCC GCTGCCAGCA GGACCACAAA GTGTTACTGA AGCGTGGACA GCCTCCTCCA 
GCGTCCTGTA TGCTGCCGGG GGGAGGAGCA GGGACTCCCC CCTCAACCAC TGCTCCAGGG 
GCCGGCCCAG GCAACGCACA GCCAGGGGCG GGGACACTGC TGCCAGGGGA AAGCGGGGCT 
TCAACAGACT CATCATCGGG GGCAGCTGCT ACTTCAAATT ATGCAAATTC CACTTGGGGC 
TCAGGAGGCC CCACCAGCAG CGGCCCCACC ACAAACCCTC CCCACGCCTG GGACAAAGTG 
ATTGTAGACG GGTCGGACAT GGAAGAGTGG CCTTGCATAG CCGGCGTGGA CGCAGAGCCC 
CCTTCCGACA ACGCCACAGA CAACAACAGT GCCTCGAACT CAGCCTCGGA GAAGGGACCC 
CCACAAGGAA GCACCACTAG CCACAAAGGG CGAGGGGGCC AGGGCCAGCA GCAACAGCAG 
CAGCAGCCTG GTAGTGAATG TATCCAGGGC GTCTGGAAAT CTGACCCCAA GGCTAAGCCC 
GTCCCGTCTT CCAACCCTGC TTCAGAGGGT GTAAATAGAC TGGGAAACTG GAGAAATGGG 
GGTGGGTCAG AGAGAGCAGG TTCTGCCTCT GGTTTCAGCA ACTTTAATCC AAATACTAAC 
CCATCTGCTT GGCCTGCCCT TGTCCAGGAA GGCAACAGGA AAGGGAACCC AGAGTCAGAC 
AGCAGCTTTT CTGGCGCACA ACTCGGCCCA GTGGGTTTGA CCTCTCGGGA GCAGCAGTCA 
AAGATGGAAA ATGCAGGTTC CAGCTTTGCA GGATCGGGCA GGGAGTCTGC CACAACCCAT 
CACACTGACG GACCAAAAAA TGGAAACACT AACTCTACGA ACTCAAATAC TTTGAACCCT 
CCTGATAACA AGGGAACAGC TTTTGGGGTG GGCTCAATGG ATTCCTGCCG GGGTTCAGAC 
TCCCCTGCTC AAAGCACTGG AGAAAGAAAG AATGGGGGGA TTGGCTCTTG GGGAGCGGGA 
AGGGGTCAGT CTGCCACAGG AGATGCTAAT TGTGGACATA GCAACTCTGG GAGCAATGGA 
CGAGAGAGAG AAGACGCAAG GAAAGCGCCT TCATCTCATA GACCCAATGG GTCAAGGGGG 
GAATCTTGGG AAAGCAAAAG TGGGAATGGT TCTTGGGGTT ACAGCCCGCA GGCAGACCTC 
AGTGAAGTGA AATGGAGTGA AGGGAACAAA TTGGGGGCTG GAGGGGCGTC TCAGGGAGAA 
TGGAAGCAGC CAGCCGGGCA TGAGGACTTA AAGGTGGGAG AGTGGGGAGG CTTGAACCAA 
GGAAACTCTA GCACTGGAGC ATGGGACAAT CAGAAGGGCC ACTCTCTATC GGATAACCAG 
GGCAACACGT CATCAGCAGC CTGCTGGGGC CGAGCCCCCA GCTCTGCAGG AAGTGAAGCT 
GGGGGGCAAA GCACTGGAAG CAACCCTAAA GCAGGGGGGA GCGGAGGAAG CGGAGACAGC 
CTGAACTCCA GCAGTCGACG TTCACACAGA CCTGCCCTCC CAGACAGTCA GGCTGCTCTT 
CAGACTTTAC TGAATCGCAC TGATTTGGAT CCCCGGGTGC TGTCCAACTC TGGCTGGGGC 
CAAACCCAGA TCAAGCAAGA TGCATCATGG GAGAGTGAGG AAGTGCCCTC TCGAACTGAA 
GGCAAATCAG ACCGTGGGAC TGAAGGTTGG GAGAACGTGT CCCAAGCCAA AACCTCAGGG 
GGTTGGGGGG ATGCTCCCAG CCAAAGCAAT CAAAACAAGT CAGGCTGGGG GGAGCCCCCT 
GTCCCTGCAC CTTCAACCCA AGAATGGAAG GACACCAAAG CAACTGGCTG GAATGACTAT 
AAAAACAGCC CAACTGGTTG GGTTGGTGGA GGAGGTGGTG GGGTGGTTGG AGCACGACCA 
GAGGACAAGG CAGTTCCAGG TTGGAACAAT GAAAGTGGTG GGGCTGCGGA GCCTGGGTGG 
AGTACTCGCC AGTCTAATCC AGGAGGTGGC TGGTCAGCAG GAAAGAATGG TTGGGGGGGA 
AATGGTAGTG AGGACATGGA GCCTCCCAAG GCCCCTGGAG GCTGGGAAGG CTCAGGAAGC 
AAATCCTCCT CTGGCTGGGG TGAAGTTGGA CAAAATGATG TGGGTTCTTG GGGTAATGGG 
GCAAACTCAA ATCCAGCTCC ACGAACTGGC TGGGAGGAGA CTAAGAGGCC CCAGGGAGGA 
GGAAACTGGG GTGAGGCAGC TAGGCCCCAA CTACCCTGGA ACAAACAGCA GGAATCAACG 
CCATCTTCAG CAACTCCTAG CACCTGGGGG GGCTCTCAGG CTCCCCCGCC ACCTGGAAAT 
GGGAGGTCAC AGCCAACTTC AGGGTGGAAG GGTGGTCCCC AGCCAACTGT TTCCAAGGAG 
GAAGAGCCAA GTGGCTGGGA GGAACCATCC TCACAATCCA TTGGTCATAA AATGGACATT 
GATGACGGAA CAGCAGCTTG GGGGGATCCT AACAATTACA ATTACAAAAA TGTCAGCCTG 
TGGGACAAGA ATGCTCAAGC AGGCCCTGGG CAGATGGCTC GGGAGCAAGG AATATCTGGG 
ACATTGGCAA ACAAGGCAGC TCCCTCAGTG TGGAGTAAAA ACAAAGGACC CCCAACTGAC 
AATGGGACAT CAGCTTGGGG TGAGCCAACT GAACCTGCTT CAGGGTGGGG AGAAGGTGAG 
GAGTCGGGTC CACCAACACC AGGATGGGGA AGTGCACCCC AAGTAAATCC CAGTGCCATG 
AAATCTGGTA CAAAATCTAT GCAAGATGGC TGGGGTGAGA GTGATGGGCC GGTGACAGGA 
ACACGTCACA GCAGCTGGGA TGAAGAAGAA GAGGGTGGAG TCTGGAACAG TGCAGGTTCT 
CAGGGAAGTG GCTCCTCCCA CAACTCTGCA GGTTGGGGAC AAGGAGGAAA GAAACCACAG 
ATCAAGGGTC CCTTAAAAGG AGGGAGCAAT GATACCTGGA TGAACACCTT GCCCAAACAG 
TTCTCCAACA TGGGATTGTT GAGCCAAGTT GATGACCCCT CTACCACTAA GATGGACCAT 
CCTGGCGGGC TTCCAGATAA GAAGTTTGAG ACAGACAAGC GGGGCGTGAA TCTCGGGGAT 
TTTAATGACA TGATGCGGAA GGAACGGTCT GGCTTCCGTC TGAACAATTC CAAAGAGATG 
GGAGCTGCAG ACAGTGGGCC TTATTTTGAG AAGCTGACTT TGCCTTTCTC CAATCAAGAC 
GGGTGCCTTG GGGATGAGGC TCCCTGTTCC CCTTTTTCTC CCTCCCCTAG CTACAAGCTA 
TCTCCCTCCG GCTCCTCGCT CCCTTCCGTG GGCCTGGGGG CTATCGGTTC TGGCCTCAAC 
CCCCAAACCT TCGCTGCTAG ACAAGGTGGC AATCACGGAC TATTTGGAAA CAGCGGTGCA 
CAATCGCGAG GCATGCACAA CCCTGTGCCA TCGCTCAACT CCTCTCCCAA CCTCCGGGCA 
CAAGTGCCTC CCCAGTTCCT CTCTCCCCAG GTCTCAGCCT CCATGCTAAA GCAGTTTCCT 
AACAGTAACA TGAATCCAGG ACTTTTCAAT ATGGGGCCAC AGCTTTCCCC TCAGCAGATT 
GCAATGCTGA GCCAGCTTCC TCAAATTCAG CAGTTCCAGC TGGCATGTCA GCTCCTCTTG 
CAACAACAAC AACAGCAGCA GCAACAGCAG CAGCAGCAGC AGCAACTCCT TCAGAACCAG 
AGAAAGATCT CACAAGCGGT CAGACAGCAG CAGGAACAGC AGCAGATAGC ACGCATGGTA 
AGTGCCCTAC AACAGCAGCA ACAGCAACAG CAGCAGCAGC AACAGAGGCA GCCAGGAATG 
AAGCACTCTC CCTCTCACCC TGCTGGACCC AAAGGTCACT TAGATGCTGT TGGACTCTCA 
GACATGCAAG CCAAAGGGCA CATGCCATAT GGATCTGGTT TTGGCTCAAG TGGGATGGAT 
TATGGTACAG TAGGTAAGGA GGCGGGAGCA GAGTCTCGAT TTAAACAGTG GACATCCATG 
ATGGAGGGGC TACCGTCCAC TGTTTCTCAA GATACCAGCC TGCATAAAAA TGGCTCCATT 
GCACCCCCAG TAAAAAATCG TGGAGGTTCC CCCTACCACC AATATGACAT GTTGTCTACG 
GATGCTTTGG GAGGGCATGG GGGGCCAACA GCTGAGAGCT GGTTACCTGC CAAATCACCC 
CCTTCAAGCA AAATTGGAAA CAAGTCAATC AATGCCAGCT GGCCCCCAGA GTTTCAGCCA 
GGAGTGCCAT GGAAAGGGAT CCAAAATATT GATCCAGAAT CTGATCCTTA TGTTACCCCT 
GGCAGTGTGC TTGGGGGTCC AGCCCCATCT CCCATTGTAG ATACTGACCA CCAGCTACTG 
CAGGACACCC CCACAGGGTC CAATTCTTCC CTCAACACCT CGCTGCCTTC ACCTGGTGCC 
TGGCCCTTTA GTGCCTCTGA GAATATCCAT AGCACTTCAG CACTCTCTGC AAAGTTTGGG 
GACTACAAAT CGACCTGGTC ACCAGACCCC ATAGGACATA ACCCATCACA TCACTCAAAC 
AAGATGTGGA AAAACCAGAT GTCCAGGAAC AGCCCTGGGC TGCCTCGCCC ACCTCCAGGG 
CTCACAAACA ACCCCAAAGC CACCAACTCT CCCTGGAACA GCACAGCACC TCGCTCCGTC 
AGGGGCTGGG GCGGACAGGA CTCCAGAATG GCTTCTGCAT CAGCTTGGAG TGATGGTGGA 
TCTGTGCGCC CCAGCTACTG GCTGATCCTT CTTAATCTCA CACCACAGAT CGATGGTTCC 
ACCCTGCGAA CGATCTGCAT GCAGCATGGC CCCCTGCTGA CATTCCATCT CAACCTTACC 
CAGGGCACAG CTCTAATCCG ATACAGCACC AAACAGGAGG CAGCCAAGGC CCAGAGTGCC 
CTCCATATGT GTGTTCTGGG TAATACAACT ATCCTGGCGG AGTTTGCAAC GGATGAGGAG 
GTGAGCCGGT ATCTTGCACA AGCTCAGCCA CCAGCTCCCT CTGCTCCAGG AGCAGGCTGG 
CAGCCTTTGG AAGTGGGACC GAACCACCCA GCTGAACCAG TAGGGCCAGC CCTAAACCTT 
TTTGGAGGAA CCACAGGGCT AGGGCAATGG AGCAGCGGCG GTGGTGGTAG TGACCTGTCA 
GGGGTTTCTC TCTGGGGTAC TCCTAGCAGC TACACATCCA GTCTGTGGGG TGTGCCGAGC 
ACAGAGGACG CCCACAGGAT GGGCAGCCCT GCCCCGTTAC TACCCGGTGA CCTTCTGGGA 
GGAGGGACCG ACTCGATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnrc6b ( XM_018093653.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949142.1 Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X1, mRNA.

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CloneID	OXa44221
Clone ID Related Accession (Same CDS sequence)	XM_018093653.1
Accession Version	XM_018093653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trinucleotide repeat-containing gene 6B protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAATG GCTGCCAGTC TCTCGTTTAT GCACATTTCC CTTTAAAGAG GAGAAAACAG 
AAATATATAA ACAAAGAGAA GGAGCAAGAA AGGGAAGAAC AGTTAATGGA AGACAAGAAA 
AGGAAGAAAG AGGATAAAAA GAAGAAAGAA GCTGCTCAGA AGGTCACAGA ACAAAAAACC 
AAAGTGCCCG AAGTGACGAA ACCAAGTTCA AGCCAAGCGA CGGCCGCCAG CCTACCTGGC 
AGCTCCCCCT CGCCACCAGT CAATGGTGGC AACAATGCCA AAAGGGTGGC AGTGCCGAAC 
GGACAACCGC CCAGCACCGC CCGCTATATG CCTCGGGAGG TGCCGCCGCG ATTCCGCTGC 
CAGCAGGACC ACAAAGTGTT ACTGAAGCGT GGACAGCCTC CTCCAGCGTC CTGTATGCTG 
CCGGGGGGAG GAGCAGGGAC TCCCCCCTCA ACCACTGCTC CAGGGGCCGG CCCAGGCAAC 
GCACAGCCAG GGGCGGGGAC ACTGCTGCCA GGGGAAAGCG GGGCTTCAAC AGACTCATCA 
TCGGGGGCAG CTGCTACTTC AAATTATGCA AATTCCACTT GGGGCTCAGG AGGCCCCACC 
AGCAGCGGCC CCACCACAAA CCCTCCCCAC GCCTGGGACA AAGTGATTGT AGACGGGTCG 
GACATGGAAG AGTGGCCTTG CATAGCCGGC GTGGACGCAG AGCCCCCTTC CGACAACGCC 
ACAGACAACA ACAGTGCCTC GAACTCAGCC TCGGAGAAGG GACCCCCACA AGGAAGCACC 
ACTAGCCACA AAGGGCGAGG GGGCCAGGGC CAGCAGCAAC AGCAGCAGCA GCCTGGTAGT 
GAATGTATCC AGGGCGTCTG GAAATCTGAC CCCAAGGCTA AGCCCGTCCC GTCTTCCAAC 
CCTGCTTCAG AGGGTGTAAA TAGACTGGGA AACTGGAGAA ATGGGGGTGG GTCAGAGAGA 
GCAGGTTCTG CCTCTGGTTT CAGCAACTTT AATCCAAATA CTAACCCATC TGCTTGGCCT 
GCCCTTGTCC AGGAAGGCAA CAGGAAAGGG AACCCAGAGT CAGACAGCAG CTTTTCTGGC 
GCACAACTCG GCCCAGTGGG TTTGACCTCT CGGGAGCAGC AGTCAAAGAT GGAAAATGCA 
GGTTCCAGCT TTGCAGGATC GGGCAGGGAG TCTGCCACAA CCCATCACAC TGACGGACCA 
AAAAATGGAA ACACTAACTC TACGAACTCA AATACTTTGA ACCCTCCTGA TAACAAGGGA 
ACAGCTTTTG GGGTGGGCTC AATGGATTCC TGCCGGGGTT CAGACTCCCC TGCTCAAAGC 
ACTGGAGAAA GAAAGAATGG GGGGATTGGC TCTTGGGGAG CGGGAAGGGG TCAGTCTGCC 
ACAGGAGATG CTAATTGTGG ACATAGCAAC TCTGGGAGCA ATGGACGAGA GAGAGAAGAC 
GCAAGGAAAG CGCCTTCATC TCATAGACCC AATGGGTCAA GGGGGGAATC TTGGGAAAGC 
AAAAGTGGGA ATGGTTCTTG GGGTTACAGC CCGCAGGCAG ACCTCAGTGA AGTGAAATGG 
AGTGAAGGGA ACAAATTGGG GGCTGGAGGG GCGTCTCAGG GAGAATGGAA GCAGCCAGCC 
GGGCATGAGG ACTTAAAGGT GGGAGAGTGG GGAGGCTTGA ACCAAGGAAA CTCTAGCACT 
GGAGCATGGG ACAATCAGAA GGGCCACTCT CTATCGGATA ACCAGGGCAA CACGTCATCA 
GCAGCCTGCT GGGGCCGAGC CCCCAGCTCT GCAGGAAGTG AAGCTGGGGG GCAAAGCACT 
GGAAGCAACC CTAAAGCAGG GGGGAGCGGA GGAAGCGGAG ACAGCCTGAA CTCCAGCAGT 
CGACGTTCAC ACAGACCTGC CCTCCCAGAC AGTCAGGCTG CTCTTCAGAC TTTACTGAAT 
CGCACTGATT TGGATCCCCG GGTGCTGTCC AACTCTGGCT GGGGCCAAAC CCAGATCAAG 
CAAGATGCAT CATGGGAGAG TGAGGAAGTG CCCTCTCGAA CTGAAGGCAA ATCAGACCGT 
GGGACTGAAG GTTGGGAGAA CGTGTCCCAA GCCAAAACCT CAGGGGGTTG GGGGGATGCT 
CCCAGCCAAA GCAATCAAAA CAAGTCAGGC TGGGGGGAGC CCCCTGTCCC TGCACCTTCA 
ACCCAAGAAT GGAAGGACAC CAAAGCAACT GGCTGGAATG ACTATAAAAA CAGCCCAACT 
GGTTGGGTTG GTGGAGGAGG TGGTGGGGTG GTTGGAGCAC GACCAGAGGA CAAGGCAGTT 
CCAGGTTGGA ACAATGAAAG TGGTGGGGCT GCGGAGCCTG GGTGGAGTAC TCGCCAGTCT 
AATCCAGGAG GTGGCTGGTC AGCAGGAAAG AATGGTTGGG GGGGAAATGG TAGTGAGGAC 
ATGGAGCCTC CCAAGGCCCC TGGAGGCTGG GAAGGCTCAG GAAGCAAATC CTCCTCTGGC 
TGGGGTGAAG TTGGACAAAA TGATGTGGGT TCTTGGGGTA ATGGGGCAAA CTCAAATCCA 
GCTCCACGAA CTGGCTGGGA GGAGACTAAG AGGCCCCAGG GAGGAGGAAA CTGGGGTGAG 
GCAGCTAGGC CCCAACTACC CTGGAACAAA CAGCAGGAAT CAACGCCATC TTCAGCAACT 
CCTAGCACCT GGGGGGGCTC TCAGGCTCCC CCGCCACCTG GAAATGGGAG GTCACAGCCA 
ACTTCAGGGT GGAAGGGTGG TCCCCAGCCA ACTGTTTCCA AGGAGGAAGA GCCAAGTGGC 
TGGGAGGAAC CATCCTCACA ATCCATTGGT CATAAAATGG ACATTGATGA CGGAACAGCA 
GCTTGGGGGG ATCCTAACAA TTACAATTAC AAAAATGTCA GCCTGTGGGA CAAGAATGCT 
CAAGCAGGCC CTGGGCAGAT GGCTCGGGAG CAAGGAATAT CTGGGACATT GGCAAACAAG 
GCAGCTCCCT CAGTGTGGAG TAAAAACAAA GGACCCCCAA CTGACAATGG GACATCAGCT 
TGGGGTGAGC CAACTGAACC TGCTTCAGGG TGGGGAGAAG GTGAGGAGTC GGGTCCACCA 
ACACCAGGAT GGGGAAGTGC ACCCCAAGTA AATCCCAGTG CCATGAAATC TGGTACAAAA 
TCTATGCAAG ATGGCTGGGG TGAGAGTGAT GGGCCGGTGA CAGGAACACG TCACAGCAGC 
TGGGATGAAG AAGAAGAGGG TGGAGTCTGG AACAGTGCAG GTTCTCAGGG AAGTGGCTCC 
TCCCACAACT CTGCAGGTTG GGGACAAGGA GGAAAGAAAC CACAGATCAA GGGTCCCTTA 
AAAGGAGGGA GCAATGATAC CTGGATGAAC ACCTTGCCCA AACAGTTCTC CAACATGGGA 
TTGTTGAGCC AAGTTGATGA CCCCTCTACC ACTAAGATGG ACCATCCTGG CGGGCTTCCA 
GATAAGAAGT TTGAGACAGA CAAGCGGGGC GTGAATCTCG GGGATTTTAA TGACATGATG 
CGGAAGGAAC GGTCTGGCTT CCGTCTGAAC AATTCCAAAG AGATGGGAGC TGCAGACAGT 
GGGCCTTATT TTGAGAAGCT GACTTTGCCT TTCTCCAATC AAGACGGGTG CCTTGGGGAT 
GAGGCTCCCT GTTCCCCTTT TTCTCCCTCC CCTAGCTACA AGCTATCTCC CTCCGGCTCC 
TCGCTCCCTT CCGTGGGCCT GGGGGCTATC GGTTCTGGCC TCAACCCCCA AACCTTCGCT 
GCTAGACAAG GTGGCAATCA CGGACTATTT GGAAACAGCG GTGCACAATC GCGAGGCATG 
CACAACCCTG TGCCATCGCT CAACTCCTCT CCCAACCTCC GGGCACAAGT GCCTCCCCAG 
TTCCTCTCTC CCCAGGTCTC AGCCTCCATG CTAAAGCAGT TTCCTAACAG TAACATGAAT 
CCAGGACTTT TCAATATGGG GCCACAGCTT TCCCCTCAGC AGATTGCAAT GCTGAGCCAG 
CTTCCTCAAA TTCAGCAGTT CCAGCTGGCA TGTCAGCTCC TCTTGCAACA ACAACAACAG 
CAGCAGCAAC AGCAGCAGCA GCAGCAGCAA CTCCTTCAGA ACCAGAGAAA GATCTCACAA 
GCGGTCAGAC AGCAGCAGGA ACAGCAGCAG ATAGCACGCA TGGTAAGTGC CCTACAACAG 
CAGCAACAGC AACAGCAGCA GCAGCAACAG AGGCAGCCAG GAATGAAGCA CTCTCCCTCT 
CACCCTGCTG GACCCAAAGG TCACTTAGAT GCTGTTGGAC TCTCAGACAT GCAAGCCAAA 
GGGCACATGC CATATGGATC TGGTTTTGGC TCAAGTGGGA TGGATTATGG TACAGTAGGT 
AAGGAGGCGG GAGCAGAGTC TCGATTTAAA CAGTGGACAT CCATGATGGA GGGGCTACCG 
TCCACTGTTT CTCAAGATAC CAGCCTGCAT AAAAATGGCT CCATTGCACC CCCAGTAAAA 
AATCGTGGAG GTTCCCCCTA CCACCAATAT GACATGTTGT CTACGGATGC TTTGGGAGGG 
CATGGGGGGC CAACAGCTGA GAGCTGGTTA CCTGCCAAAT CACCCCCTTC AAGCAAAATT 
GGAAACAAGT CAATCAATGC CAGCTGGCCC CCAGAGTTTC AGCCAGGAGT GCCATGGAAA 
GGGATCCAAA ATATTGATCC AGAATCTGAT CCTTATGTTA CCCCTGGCAG TGTGCTTGGG 
GGTCCAGCCC CATCTCCCAT TGTAGATACT GACCACCAGC TACTGCAGGA CACCCCCACA 
GGGTCCAATT CTTCCCTCAA CACCTCGCTG CCTTCACCTG GTGCCTGGCC CTTTAGTGCC 
TCTGAGAATA TCCATAGCAC TTCAGCACTC TCTGCAAAGT TTGGGGACTA CAAATCGACC 
TGGTCACCAG ACCCCATAGG ACATAACCCA TCACATCACT CAAACAAGAT GTGGAAAAAC 
CAGATGTCCA GGAACAGCCC TGGGCTGCCT CGCCCACCTC CAGGGCTCAC AAACAACCCC 
AAAGCCACCA ACTCTCCCTG GAACAGCACA GCACCTCGCT CCGTCAGGGG CTGGGGCGGA 
CAGGACTCCA GAATGGCTTC TGCATCAGCT TGGAGTGATG GTGGATCTGT GCGCCCCAGC 
TACTGGCTGA TCCTTCTTAA TCTCACACCA CAGATCGATG GTTCCACCCT GCGAACGATC 
TGCATGCAGC ATGGCCCCCT GCTGACATTC CATCTCAACC TTACCCAGGG CACAGCTCTA 
ATCCGATACA GCACCAAACA GGAGGCAGCC AAGGCCCAGA GTGCCCTCCA TATGTGTGTT 
CTGGGTAATA CAACTATCCT GGCGGAGTTT GCAACGGATG AGGAGGTGAG CCGGTATCTT 
GCACAAGCTC AGCCACCAGC TCCCTCTGCT CCAGGAGCAG GCTGGCAGCC TTTGGAAGTG 
GGACCGAACC ACCCAGCTGA ACCAGTAGGG CCAGCCCTAA ACCTTTTTGG AGGAACCACA 
GGGCTAGGGC AATGGAGCAG CGGCGGTGGT GGTAGTGACC TGTCAGGGGT TTCTCTCTGG 
GGTACTCCTA GCAGCTACAC ATCCAGTCTG TGGGGTGTGC CGAGCACAGA GGACGCCCAC 
AGGATGGGCA GCCCTGCCCC GTTACTACCC GGTGACCTTC TGGGAGGAGG GACCGACTCG 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949142.1
CDS	49..5694
Translation	MQNGCQSLVYAHFPLKRRKQKYINKEKEQEREEQLMEDKKRKKEDKKKKEAAQKVTEQKTKVPEVTKPSSSQATAASLPGSSPSPPVNGGNNAKRVAVPNGQPPSTARYMPREVPPRFRCQQDHKVLLKRGQPPPASCMLPGGGAGTPPSTTAPGAGPGNAQPGAGTLLPGESGASTDSSSGAAATSNYANSTWGSGGPTSSGPTTNPPHAWDKVIVDGSDMEEWPCIAGVDAEPPSDNATDNNSASNSASEKGPPQGSTTSHKGRGGQGQQQQQQQPGSECIQGVWKSDPKAKPVPSSNPASEGVNRLGNWRNGGGSERAGSASGFSNFNPNTNPSAWPALVQEGNRKGNPESDSSFSGAQLGPVGLTSREQQSKMENAGSSFAGSGRESATTHHTDGPKNGNTNSTNSNTLNPPDNKGTAFGVGSMDSCRGSDSPAQSTGERKNGGIGSWGAGRGQSATGDANCGHSNSGSNGREREDARKAPSSHRPNGSRGESWESKSGNGSWGYSPQADLSEVKWSEGNKLGAGGASQGEWKQPAGHEDLKVGEWGGLNQGNSSTGAWDNQKGHSLSDNQGNTSSAACWGRAPSSAGSEAGGQSTGSNPKAGGSGGSGDSLNSSSRRSHRPALPDSQAALQTLLNRTDLDPRVLSNSGWGQTQIKQDASWESEEVPSRTEGKSDRGTEGWENVSQAKTSGGWGDAPSQSNQNKSGWGEPPVPAPSTQEWKDTKATGWNDYKNSPTGWVGGGGGGVVGARPEDKAVPGWNNESGGAAEPGWSTRQSNPGGGWSAGKNGWGGNGSEDMEPPKAPGGWEGSGSKSSSGWGEVGQNDVGSWGNGANSNPAPRTGWEETKRPQGGGNWGEAARPQLPWNKQQESTPSSATPSTWGGSQAPPPPGNGRSQPTSGWKGGPQPTVSKEEEPSGWEEPSSQSIGHKMDIDDGTAAWGDPNNYNYKNVSLWDKNAQAGPGQMAREQGISGTLANKAAPSVWSKNKGPPTDNGTSAWGEPTEPASGWGEGEESGPPTPGWGSAPQVNPSAMKSGTKSMQDGWGESDGPVTGTRHSSWDEEEEGGVWNSAGSQGSGSSHNSAGWGQGGKKPQIKGPLKGGSNDTWMNTLPKQFSNMGLLSQVDDPSTTKMDHPGGLPDKKFETDKRGVNLGDFNDMMRKERSGFRLNNSKEMGAADSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSSLPSVGLGAIGSGLNPQTFAARQGGNHGLFGNSGAQSRGMHNPVPSLNSSPNLRAQVPPQFLSPQVSASMLKQFPNSNMNPGLFNMGPQLSPQQIAMLSQLPQIQQFQLACQLLLQQQQQQQQQQQQQQQLLQNQRKISQAVRQQQEQQQIARMVSALQQQQQQQQQQQQRQPGMKHSPSHPAGPKGHLDAVGLSDMQAKGHMPYGSGFGSSGMDYGTVGKEAGAESRFKQWTSMMEGLPSTVSQDTSLHKNGSIAPPVKNRGGSPYHQYDMLSTDALGGHGGPTAESWLPAKSPPSSKIGNKSINASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGPAPSPIVDTDHQLLQDTPTGSNSSLNTSLPSPGAWPFSASENIHSTSALSAKFGDYKSTWSPDPIGHNPSHHSNKMWKNQMSRNSPGLPRPPPGLTNNPKATNSPWNSTAPRSVRGWGGQDSRMASASAWSDGGSVRPSYWLILLNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQSALHMCVLGNTTILAEFATDEEVSRYLAQAQPPAPSAPGAGWQPLEVGPNHPAEPVGPALNLFGGTTGLGQWSSGGGGSDLSGVSLWGTPSSYTSSLWGVPSTEDAHRMGSPAPLLPGDLLGGGTDSI

Target ORF information:

RefSeq Version	XM_018093653.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093653.1

ORF Insert Sequence:

ATGCAAAATG GCTGCCAGTC TCTCGTTTAT GCACATTTCC CTTTAAAGAG GAGAAAACAG 
AAATATATAA ACAAAGAGAA GGAGCAAGAA AGGGAAGAAC AGTTAATGGA AGACAAGAAA 
AGGAAGAAAG AGGATAAAAA GAAGAAAGAA GCTGCTCAGA AGGTCACAGA ACAAAAAACC 
AAAGTGCCCG AAGTGACGAA ACCAAGTTCA AGCCAAGCGA CGGCCGCCAG CCTACCTGGC 
AGCTCCCCCT CGCCACCAGT CAATGGTGGC AACAATGCCA AAAGGGTGGC AGTGCCGAAC 
GGACAACCGC CCAGCACCGC CCGCTATATG CCTCGGGAGG TGCCGCCGCG ATTCCGCTGC 
CAGCAGGACC ACAAAGTGTT ACTGAAGCGT GGACAGCCTC CTCCAGCGTC CTGTATGCTG 
CCGGGGGGAG GAGCAGGGAC TCCCCCCTCA ACCACTGCTC CAGGGGCCGG CCCAGGCAAC 
GCACAGCCAG GGGCGGGGAC ACTGCTGCCA GGGGAAAGCG GGGCTTCAAC AGACTCATCA 
TCGGGGGCAG CTGCTACTTC AAATTATGCA AATTCCACTT GGGGCTCAGG AGGCCCCACC 
AGCAGCGGCC CCACCACAAA CCCTCCCCAC GCCTGGGACA AAGTGATTGT AGACGGGTCG 
GACATGGAAG AGTGGCCTTG CATAGCCGGC GTGGACGCAG AGCCCCCTTC CGACAACGCC 
ACAGACAACA ACAGTGCCTC GAACTCAGCC TCGGAGAAGG GACCCCCACA AGGAAGCACC 
ACTAGCCACA AAGGGCGAGG GGGCCAGGGC CAGCAGCAAC AGCAGCAGCA GCCTGGTAGT 
GAATGTATCC AGGGCGTCTG GAAATCTGAC CCCAAGGCTA AGCCCGTCCC GTCTTCCAAC 
CCTGCTTCAG AGGGTGTAAA TAGACTGGGA AACTGGAGAA ATGGGGGTGG GTCAGAGAGA 
GCAGGTTCTG CCTCTGGTTT CAGCAACTTT AATCCAAATA CTAACCCATC TGCTTGGCCT 
GCCCTTGTCC AGGAAGGCAA CAGGAAAGGG AACCCAGAGT CAGACAGCAG CTTTTCTGGC 
GCACAACTCG GCCCAGTGGG TTTGACCTCT CGGGAGCAGC AGTCAAAGAT GGAAAATGCA 
GGTTCCAGCT TTGCAGGATC GGGCAGGGAG TCTGCCACAA CCCATCACAC TGACGGACCA 
AAAAATGGAA ACACTAACTC TACGAACTCA AATACTTTGA ACCCTCCTGA TAACAAGGGA 
ACAGCTTTTG GGGTGGGCTC AATGGATTCC TGCCGGGGTT CAGACTCCCC TGCTCAAAGC 
ACTGGAGAAA GAAAGAATGG GGGGATTGGC TCTTGGGGAG CGGGAAGGGG TCAGTCTGCC 
ACAGGAGATG CTAATTGTGG ACATAGCAAC TCTGGGAGCA ATGGACGAGA GAGAGAAGAC 
GCAAGGAAAG CGCCTTCATC TCATAGACCC AATGGGTCAA GGGGGGAATC TTGGGAAAGC 
AAAAGTGGGA ATGGTTCTTG GGGTTACAGC CCGCAGGCAG ACCTCAGTGA AGTGAAATGG 
AGTGAAGGGA ACAAATTGGG GGCTGGAGGG GCGTCTCAGG GAGAATGGAA GCAGCCAGCC 
GGGCATGAGG ACTTAAAGGT GGGAGAGTGG GGAGGCTTGA ACCAAGGAAA CTCTAGCACT 
GGAGCATGGG ACAATCAGAA GGGCCACTCT CTATCGGATA ACCAGGGCAA CACGTCATCA 
GCAGCCTGCT GGGGCCGAGC CCCCAGCTCT GCAGGAAGTG AAGCTGGGGG GCAAAGCACT 
GGAAGCAACC CTAAAGCAGG GGGGAGCGGA GGAAGCGGAG ACAGCCTGAA CTCCAGCAGT 
CGACGTTCAC ACAGACCTGC CCTCCCAGAC AGTCAGGCTG CTCTTCAGAC TTTACTGAAT 
CGCACTGATT TGGATCCCCG GGTGCTGTCC AACTCTGGCT GGGGCCAAAC CCAGATCAAG 
CAAGATGCAT CATGGGAGAG TGAGGAAGTG CCCTCTCGAA CTGAAGGCAA ATCAGACCGT 
GGGACTGAAG GTTGGGAGAA CGTGTCCCAA GCCAAAACCT CAGGGGGTTG GGGGGATGCT 
CCCAGCCAAA GCAATCAAAA CAAGTCAGGC TGGGGGGAGC CCCCTGTCCC TGCACCTTCA 
ACCCAAGAAT GGAAGGACAC CAAAGCAACT GGCTGGAATG ACTATAAAAA CAGCCCAACT 
GGTTGGGTTG GTGGAGGAGG TGGTGGGGTG GTTGGAGCAC GACCAGAGGA CAAGGCAGTT 
CCAGGTTGGA ACAATGAAAG TGGTGGGGCT GCGGAGCCTG GGTGGAGTAC TCGCCAGTCT 
AATCCAGGAG GTGGCTGGTC AGCAGGAAAG AATGGTTGGG GGGGAAATGG TAGTGAGGAC 
ATGGAGCCTC CCAAGGCCCC TGGAGGCTGG GAAGGCTCAG GAAGCAAATC CTCCTCTGGC 
TGGGGTGAAG TTGGACAAAA TGATGTGGGT TCTTGGGGTA ATGGGGCAAA CTCAAATCCA 
GCTCCACGAA CTGGCTGGGA GGAGACTAAG AGGCCCCAGG GAGGAGGAAA CTGGGGTGAG 
GCAGCTAGGC CCCAACTACC CTGGAACAAA CAGCAGGAAT CAACGCCATC TTCAGCAACT 
CCTAGCACCT GGGGGGGCTC TCAGGCTCCC CCGCCACCTG GAAATGGGAG GTCACAGCCA 
ACTTCAGGGT GGAAGGGTGG TCCCCAGCCA ACTGTTTCCA AGGAGGAAGA GCCAAGTGGC 
TGGGAGGAAC CATCCTCACA ATCCATTGGT CATAAAATGG ACATTGATGA CGGAACAGCA 
GCTTGGGGGG ATCCTAACAA TTACAATTAC AAAAATGTCA GCCTGTGGGA CAAGAATGCT 
CAAGCAGGCC CTGGGCAGAT GGCTCGGGAG CAAGGAATAT CTGGGACATT GGCAAACAAG 
GCAGCTCCCT CAGTGTGGAG TAAAAACAAA GGACCCCCAA CTGACAATGG GACATCAGCT 
TGGGGTGAGC CAACTGAACC TGCTTCAGGG TGGGGAGAAG GTGAGGAGTC GGGTCCACCA 
ACACCAGGAT GGGGAAGTGC ACCCCAAGTA AATCCCAGTG CCATGAAATC TGGTACAAAA 
TCTATGCAAG ATGGCTGGGG TGAGAGTGAT GGGCCGGTGA CAGGAACACG TCACAGCAGC 
TGGGATGAAG AAGAAGAGGG TGGAGTCTGG AACAGTGCAG GTTCTCAGGG AAGTGGCTCC 
TCCCACAACT CTGCAGGTTG GGGACAAGGA GGAAAGAAAC CACAGATCAA GGGTCCCTTA 
AAAGGAGGGA GCAATGATAC CTGGATGAAC ACCTTGCCCA AACAGTTCTC CAACATGGGA 
TTGTTGAGCC AAGTTGATGA CCCCTCTACC ACTAAGATGG ACCATCCTGG CGGGCTTCCA 
GATAAGAAGT TTGAGACAGA CAAGCGGGGC GTGAATCTCG GGGATTTTAA TGACATGATG 
CGGAAGGAAC GGTCTGGCTT CCGTCTGAAC AATTCCAAAG AGATGGGAGC TGCAGACAGT 
GGGCCTTATT TTGAGAAGCT GACTTTGCCT TTCTCCAATC AAGACGGGTG CCTTGGGGAT 
GAGGCTCCCT GTTCCCCTTT TTCTCCCTCC CCTAGCTACA AGCTATCTCC CTCCGGCTCC 
TCGCTCCCTT CCGTGGGCCT GGGGGCTATC GGTTCTGGCC TCAACCCCCA AACCTTCGCT 
GCTAGACAAG GTGGCAATCA CGGACTATTT GGAAACAGCG GTGCACAATC GCGAGGCATG 
CACAACCCTG TGCCATCGCT CAACTCCTCT CCCAACCTCC GGGCACAAGT GCCTCCCCAG 
TTCCTCTCTC CCCAGGTCTC AGCCTCCATG CTAAAGCAGT TTCCTAACAG TAACATGAAT 
CCAGGACTTT TCAATATGGG GCCACAGCTT TCCCCTCAGC AGATTGCAAT GCTGAGCCAG 
CTTCCTCAAA TTCAGCAGTT CCAGCTGGCA TGTCAGCTCC TCTTGCAACA ACAACAACAG 
CAGCAGCAAC AGCAGCAGCA GCAGCAGCAA CTCCTTCAGA ACCAGAGAAA GATCTCACAA 
GCGGTCAGAC AGCAGCAGGA ACAGCAGCAG ATAGCACGCA TGGTAAGTGC CCTACAACAG 
CAGCAACAGC AACAGCAGCA GCAGCAACAG AGGCAGCCAG GAATGAAGCA CTCTCCCTCT 
CACCCTGCTG GACCCAAAGG TCACTTAGAT GCTGTTGGAC TCTCAGACAT GCAAGCCAAA 
GGGCACATGC CATATGGATC TGGTTTTGGC TCAAGTGGGA TGGATTATGG TACAGTAGGT 
AAGGAGGCGG GAGCAGAGTC TCGATTTAAA CAGTGGACAT CCATGATGGA GGGGCTACCG 
TCCACTGTTT CTCAAGATAC CAGCCTGCAT AAAAATGGCT CCATTGCACC CCCAGTAAAA 
AATCGTGGAG GTTCCCCCTA CCACCAATAT GACATGTTGT CTACGGATGC TTTGGGAGGG 
CATGGGGGGC CAACAGCTGA GAGCTGGTTA CCTGCCAAAT CACCCCCTTC AAGCAAAATT 
GGAAACAAGT CAATCAATGC CAGCTGGCCC CCAGAGTTTC AGCCAGGAGT GCCATGGAAA 
GGGATCCAAA ATATTGATCC AGAATCTGAT CCTTATGTTA CCCCTGGCAG TGTGCTTGGG 
GGTCCAGCCC CATCTCCCAT TGTAGATACT GACCACCAGC TACTGCAGGA CACCCCCACA 
GGGTCCAATT CTTCCCTCAA CACCTCGCTG CCTTCACCTG GTGCCTGGCC CTTTAGTGCC 
TCTGAGAATA TCCATAGCAC TTCAGCACTC TCTGCAAAGT TTGGGGACTA CAAATCGACC 
TGGTCACCAG ACCCCATAGG ACATAACCCA TCACATCACT CAAACAAGAT GTGGAAAAAC 
CAGATGTCCA GGAACAGCCC TGGGCTGCCT CGCCCACCTC CAGGGCTCAC AAACAACCCC 
AAAGCCACCA ACTCTCCCTG GAACAGCACA GCACCTCGCT CCGTCAGGGG CTGGGGCGGA 
CAGGACTCCA GAATGGCTTC TGCATCAGCT TGGAGTGATG GTGGATCTGT GCGCCCCAGC 
TACTGGCTGA TCCTTCTTAA TCTCACACCA CAGATCGATG GTTCCACCCT GCGAACGATC 
TGCATGCAGC ATGGCCCCCT GCTGACATTC CATCTCAACC TTACCCAGGG CACAGCTCTA 
ATCCGATACA GCACCAAACA GGAGGCAGCC AAGGCCCAGA GTGCCCTCCA TATGTGTGTT 
CTGGGTAATA CAACTATCCT GGCGGAGTTT GCAACGGATG AGGAGGTGAG CCGGTATCTT 
GCACAAGCTC AGCCACCAGC TCCCTCTGCT CCAGGAGCAG GCTGGCAGCC TTTGGAAGTG 
GGACCGAACC ACCCAGCTGA ACCAGTAGGG CCAGCCCTAA ACCTTTTTGG AGGAACCACA 
GGGCTAGGGC AATGGAGCAG CGGCGGTGGT GGTAGTGACC TGTCAGGGGT TTCTCTCTGG 
GGTACTCCTA GCAGCTACAC ATCCAGTCTG TGGGGTGTGC CGAGCACAGA GGACGCCCAC 
AGGATGGGCA GCCCTGCCCC GTTACTACCC GGTGACCTTC TGGGAGGAGG GACCGACTCG 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnrc6b ( XM_018093654.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949143.1 Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X2, mRNA.

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CloneID	OXa44222
Clone ID Related Accession (Same CDS sequence)	XM_018093654.1
Accession Version	XM_018093654.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trinucleotide repeat-containing gene 6B protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAATG GCTGCCAGTC TCTCGTTTAT GCACATTTCC CTTTAAAGAG GAGAAAACAG 
AAATATATAA ACAAAGAGAA GGAGCAAGAA AGGGAAGAAC AGTTAATGGA AGACAAGAAA 
AGGAAGAAAG AGGATAAAAA GAAGAAAGAA GCTGCTCAGA AGGTCACAGA ACAAAAAACC 
AAAGTGCCCG AAGTGACGAA ACCAAGTTCA AGCCAAGCGA CGGCCGCCAG CCTACCTGGC 
AGCTCCCCCT CGCCACCAGT CAATGGTGGC AACAATGCCA AAAGGGTGGC AGTGCCGAAC 
GGACAACCGC CCAGCACCGC CCGCTATATG CCTCGGGAGG TGCCGCCGCG ATTCCGCTGC 
CAGCAGGACC ACAAAGTGTT ACTGAAGCGT GGACAGCCTC CTCCAGCGTC CTGTATGCTG 
CCGGGGGGAG GAGCAGGGAC TCCCCCCTCA ACCACTGCTC CAGGGGCCGG CCCAGGCAAC 
GCACAGCCAG GGGCGGGGAC ACTGCTGCCA GGGGAAAGCG GGGCTTCAAC AGACTCATCA 
TCGGGGGCAG CTGCTACTTC AAATTATGCA AATTCCACTT GGGGCTCAGG AGGCCCCACC 
AGCAGCGGCC CCACCACAAA CCCTCCCCAC GCCTGGGACA AAGTGATTGT AGACGGGTCG 
GACATGGAAG AGTGGCCTTG CATAGCCGGC GTGGACGCAG AGCCCCCTTC CGACAACGCC 
ACAGACAACA ACAGTGCCTC GAACTCAGCC TCGGAGAAGG GACCCCCACA AGGAAGCACC 
ACTAGCCACA AAGGGCGAGG GGGCCAGGGC CAGCAGCAAC AGCAGCAGCA GCCTGGTAGT 
GAATGTATCC AGGGCGTCTG GAAATCTGAC CCCAAGGCTA AGCCCGTCCC GTCTTCCAAC 
CCTGCTTCAG AGGGTGTAAA TAGACTGGGA AACTGGAGAA ATGGGGGTGG GTCAGAGAGA 
GCAGGTTCTG CCTCTGGTTT CAGCAACTTT AATCCAAATA CTAACCCATC TGCTTGGCCT 
GCCCTTGTCC AGGAAGGCAA CAGGAAAGGG AACCCAGAGT CAGACAGCAG CTTTTCTGGC 
GCACAACTCG GCCCAGTGGG TTTGACCTCT CGGGAGCAGC AGTCAAAGAT GGAAAATGCA 
GGTTCCAGCT TTGCAGGATC GGGCAGGGAG TCTGCCACAA CCCATCACAC TGACGGACCA 
AAAAATGGAA ACACTAACTC TACGAACTCA AATACTTTGA ACCCTCCTGA TAACAAGGGA 
ACAGCTTTTG GGGTGGGCTC AATGGATTCC TGCCGGGGTT CAGACTCCCC TGCTCAAAGC 
ACTGGAGAAA GAAAGAATGG GGGGATTGGC TCTTGGGGAG CGGGAAGGGG TCAGTCTGCC 
ACAGGAGATG CTAATTGTGG ACATAGCAAC TCTGGGAGCA ATGGACGAGA GAGAGAAGAC 
GCAAGGAAAG CGCCTTCATC TCATAGACCC AATGGGTCAA GGGGGGAATC TTGGGAAAGC 
AAAAGTGGGA ATGGTTCTTG GGGTTACAGC CCGCAGGCAG ACCTCAGTGA AGTGAAATGG 
AGTGAAGGGA ACAAATTGGG GGCTGGAGGG GCGTCTCAGG GAGAATGGAA GCAGCCAGCC 
GGGCATGAGG ACTTAAAGGT GGGAGAGTGG GGAGGCTTGA ACCAAGGAAA CTCTAGCACT 
GGAGCATGGG ACAATCAGAA GGGCCACTCT CTATCGGATA ACCAGGGCAA CACGTCATCA 
GCAGCCTGCT GGGGCCGAGC CCCCAGCTCT GCAGGAAGTG AAGCTGGGGG GCAAAGCACT 
GGAAGCAACC CTAAAGCAGG GGGGAGCGGA GGAAGCGGAG ACAGCCTGAA CTCCAGCAGT 
CGACGTTCAC ACAGACCTGC CCTCCCAGAC AGTCAGGCTG CTCTTCAGAC TTTACTGAAT 
CGCACTGATT TGGATCCCCG GGTGCTGTCC AACTCTGGCT GGGGCCAAAC CCAGATCAAG 
CAAGATGCAT CATGGGAGAG TGAGGAAGTG CCCTCTCGAA CTGAAGGCAA ATCAGACCGT 
GGGACTGAAG GTTGGGAGAA CGTGTCCCAA GCCAAAACCT CAGGGGGTTG GGGGGATGCT 
CCCAGCCAAA GCAATCAAAA CAAGTCAGGC TGGGGGGAGC CCCCTGTCCC TGCACCTTCA 
ACCCAAGAAT GGAAGGACAC CAAAGCAACT GGCTGGAATG ACTATAAAAA CAGCCCAACT 
GGTTGGGTTG GTGGAGGAGG TGGTGGGGTG GTTGGAGCAC GACCAGAGGA CAAGGCAGTT 
CCAGGTTGGA ACAATGAAAG TGGTGGGGCT GCGGAGCCTG GGTGGAGTAC TCGCCAGTCT 
AATCCAGGAG GTGGCTGGTC AGCAGGAAAG AATGGTTGGG GGGGAAATGG TAGTGAGGAC 
ATGGAGCCTC CCAAGGCCCC TGGAGGCTGG GAAGGCTCAG GAAGCAAATC CTCCTCTGGC 
TGGGGTGAAG TTGGACAAAA TGATGTGGGT TCTTGGGGTA ATGGGGCAAA CTCAAATCCA 
GCTCCACGAA CTGGCTGGGA GGAGACTAAG AGGCCCCAGG GAGGAGGAAA CTGGGGTGAG 
GCAGCTAGGC CCCAACTACC CTGGAACAAA CAGCAGGAAT CAACGCCATC TTCAGCAACT 
CCTAGCACCT GGGGGGGCTC TCAGGCTCCC CCGCCACCTG GAAATGGGAG GTCACAGCCA 
ACTTCAGGGT GGAAGGGTGG TCCCCAGCCA ACTGTTTCCA AGGAGGAAGA GCCAAGTGGC 
TGGGAGGAAC CATCCTCACA ATCCATTGGT CATAAAATGG ACATTGATGA CGGAACAGCA 
GCTTGGGGGG ATCCTAACAA TTACAATTAC AAAAATGTCA GCCTGTGGGA CAAGAATGCT 
CAAGCAGGCC CTGGGCAGAT GGCTCGGGAG CAAGGAATAT CTGGGACATT GGCAAACAAG 
GCAGCTCCCT CAGTGTGGAG TAAAAACAAA GGACCCCCAA CTGACAATGG GACATCAGCT 
TGGGGTGAGC CAACTGAACC TGCTTCAGGG TGGGGAGAAG GTGAGGAGTC GGGTCCACCA 
ACACCAGGAT GGGGAAGTGC ACCCCAAGTA AATCCCAGTG CCATGAAATC TGGTACAAAA 
TCTATGCAAG ATGGCTGGGG TGAGAGTGAT GGGCCGGTGA CAGGAACACG TCACAGCAGC 
TGGGATGAAG AAGAAGAGGG TGGAGTCTGG AACAGTGCAG GTTCTCAGGG AAGTGGCTCC 
TCCCACAACT CTGCAGGTTG GGGACAAGGA GGAAAGAAAC CACAGATCAA GGGTCCCTTA 
AAAGGAGGGA GCAATGATAC CTGGATGAAC ACCTTGCCCA AACAGTTCTC CAACATGGGA 
TTGTTGAGCC AAGTTGATGA CCCCTCTACC ACTAAGATGG ACCATCCTGG CGGGCTTCCA 
GATAAGAAGT TTGAGACAGA CAAGCGGGGC GTGAATCTCG GGGATTTTAA TGACATGATG 
CGGAAGGAAC GGTCTGGCTT CCGTCTGAAC AATTCCAAAG AGATGGGAGC TGCAGACAGT 
GGGCCTTATT TTGAGAAGCT GACTTTGCCT TTCTCCAATC AAGACGGGTG CCTTGGGGAT 
GAGGCTCCCT GTTCCCCTTT TTCTCCCTCC CCTAGCTACA AGCTATCTCC CTCCGGCTCC 
TCGCTCCCTT CCGTGGGCCT GGGGGCTATC GGTTCTGGCC TCAACCCCCA AACCTTCGCT 
GCTAGACAAG GTGGCAATCA CGGACTATTT GGAAACAGCG GTGCACAATC GCGAGGCATG 
CACAACCCTG TGCCATCGCT CAACTCCTCT CCCAACCTCC GGGCACAAGT GCCTCCCCAG 
TTCCTCTCTC CCCAGGTCTC AGCCTCCATG CTAAAGCAGT TTCCTAACAG TAACATGAAT 
CCAGGACTTT TCAATATGGG GCCACAGCTT TCCCCTCAGC AGATTGCAAT GCTGAGCCAG 
CTTCCTCAAA TTCAGCAGTT CCAGCTGGCA TGTCAGCTCC TCTTGCAACA ACAACAACAG 
CAGCAGCAAC AGCAGCAGCA GCAGCAGCAA CTCCTTCAGA ACCAGAGAAA GATCTCACAA 
GCGGTCAGAC AGCAGCAGGA ACAGCAGATA GCACGCATGG TAAGTGCCCT ACAACAGCAG 
CAACAGCAAC AGCAGCAGCA GCAACAGAGG CAGCCAGGAA TGAAGCACTC TCCCTCTCAC 
CCTGCTGGAC CCAAAGGTCA CTTAGATGCT GTTGGACTCT CAGACATGCA AGCCAAAGGG 
CACATGCCAT ATGGATCTGG TTTTGGCTCA AGTGGGATGG ATTATGGTAC AGTAGGTAAG 
GAGGCGGGAG CAGAGTCTCG ATTTAAACAG TGGACATCCA TGATGGAGGG GCTACCGTCC 
ACTGTTTCTC AAGATACCAG CCTGCATAAA AATGGCTCCA TTGCACCCCC AGTAAAAAAT 
CGTGGAGGTT CCCCCTACCA CCAATATGAC ATGTTGTCTA CGGATGCTTT GGGAGGGCAT 
GGGGGGCCAA CAGCTGAGAG CTGGTTACCT GCCAAATCAC CCCCTTCAAG CAAAATTGGA 
AACAAGTCAA TCAATGCCAG CTGGCCCCCA GAGTTTCAGC CAGGAGTGCC ATGGAAAGGG 
ATCCAAAATA TTGATCCAGA ATCTGATCCT TATGTTACCC CTGGCAGTGT GCTTGGGGGT 
CCAGCCCCAT CTCCCATTGT AGATACTGAC CACCAGCTAC TGCAGGACAC CCCCACAGGG 
TCCAATTCTT CCCTCAACAC CTCGCTGCCT TCACCTGGTG CCTGGCCCTT TAGTGCCTCT 
GAGAATATCC ATAGCACTTC AGCACTCTCT GCAAAGTTTG GGGACTACAA ATCGACCTGG 
TCACCAGACC CCATAGGACA TAACCCATCA CATCACTCAA ACAAGATGTG GAAAAACCAG 
ATGTCCAGGA ACAGCCCTGG GCTGCCTCGC CCACCTCCAG GGCTCACAAA CAACCCCAAA 
GCCACCAACT CTCCCTGGAA CAGCACAGCA CCTCGCTCCG TCAGGGGCTG GGGCGGACAG 
GACTCCAGAA TGGCTTCTGC ATCAGCTTGG AGTGATGGTG GATCTGTGCG CCCCAGCTAC 
TGGCTGATCC TTCTTAATCT CACACCACAG ATCGATGGTT CCACCCTGCG AACGATCTGC 
ATGCAGCATG GCCCCCTGCT GACATTCCAT CTCAACCTTA CCCAGGGCAC AGCTCTAATC 
CGATACAGCA CCAAACAGGA GGCAGCCAAG GCCCAGAGTG CCCTCCATAT GTGTGTTCTG 
GGTAATACAA CTATCCTGGC GGAGTTTGCA ACGGATGAGG AGGTGAGCCG GTATCTTGCA 
CAAGCTCAGC CACCAGCTCC CTCTGCTCCA GGAGCAGGCT GGCAGCCTTT GGAAGTGGGA 
CCGAACCACC CAGCTGAACC AGTAGGGCCA GCCCTAAACC TTTTTGGAGG AACCACAGGG 
CTAGGGCAAT GGAGCAGCGG CGGTGGTGGT AGTGACCTGT CAGGGGTTTC TCTCTGGGGT 
ACTCCTAGCA GCTACACATC CAGTCTGTGG GGTGTGCCGA GCACAGAGGA CGCCCACAGG 
ATGGGCAGCC CTGCCCCGTT ACTACCCGGT GACCTTCTGG GAGGAGGGAC CGACTCGATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949143.1
CDS	49..5691
Translation	MQNGCQSLVYAHFPLKRRKQKYINKEKEQEREEQLMEDKKRKKEDKKKKEAAQKVTEQKTKVPEVTKPSSSQATAASLPGSSPSPPVNGGNNAKRVAVPNGQPPSTARYMPREVPPRFRCQQDHKVLLKRGQPPPASCMLPGGGAGTPPSTTAPGAGPGNAQPGAGTLLPGESGASTDSSSGAAATSNYANSTWGSGGPTSSGPTTNPPHAWDKVIVDGSDMEEWPCIAGVDAEPPSDNATDNNSASNSASEKGPPQGSTTSHKGRGGQGQQQQQQQPGSECIQGVWKSDPKAKPVPSSNPASEGVNRLGNWRNGGGSERAGSASGFSNFNPNTNPSAWPALVQEGNRKGNPESDSSFSGAQLGPVGLTSREQQSKMENAGSSFAGSGRESATTHHTDGPKNGNTNSTNSNTLNPPDNKGTAFGVGSMDSCRGSDSPAQSTGERKNGGIGSWGAGRGQSATGDANCGHSNSGSNGREREDARKAPSSHRPNGSRGESWESKSGNGSWGYSPQADLSEVKWSEGNKLGAGGASQGEWKQPAGHEDLKVGEWGGLNQGNSSTGAWDNQKGHSLSDNQGNTSSAACWGRAPSSAGSEAGGQSTGSNPKAGGSGGSGDSLNSSSRRSHRPALPDSQAALQTLLNRTDLDPRVLSNSGWGQTQIKQDASWESEEVPSRTEGKSDRGTEGWENVSQAKTSGGWGDAPSQSNQNKSGWGEPPVPAPSTQEWKDTKATGWNDYKNSPTGWVGGGGGGVVGARPEDKAVPGWNNESGGAAEPGWSTRQSNPGGGWSAGKNGWGGNGSEDMEPPKAPGGWEGSGSKSSSGWGEVGQNDVGSWGNGANSNPAPRTGWEETKRPQGGGNWGEAARPQLPWNKQQESTPSSATPSTWGGSQAPPPPGNGRSQPTSGWKGGPQPTVSKEEEPSGWEEPSSQSIGHKMDIDDGTAAWGDPNNYNYKNVSLWDKNAQAGPGQMAREQGISGTLANKAAPSVWSKNKGPPTDNGTSAWGEPTEPASGWGEGEESGPPTPGWGSAPQVNPSAMKSGTKSMQDGWGESDGPVTGTRHSSWDEEEEGGVWNSAGSQGSGSSHNSAGWGQGGKKPQIKGPLKGGSNDTWMNTLPKQFSNMGLLSQVDDPSTTKMDHPGGLPDKKFETDKRGVNLGDFNDMMRKERSGFRLNNSKEMGAADSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSSLPSVGLGAIGSGLNPQTFAARQGGNHGLFGNSGAQSRGMHNPVPSLNSSPNLRAQVPPQFLSPQVSASMLKQFPNSNMNPGLFNMGPQLSPQQIAMLSQLPQIQQFQLACQLLLQQQQQQQQQQQQQQQLLQNQRKISQAVRQQQEQQIARMVSALQQQQQQQQQQQQRQPGMKHSPSHPAGPKGHLDAVGLSDMQAKGHMPYGSGFGSSGMDYGTVGKEAGAESRFKQWTSMMEGLPSTVSQDTSLHKNGSIAPPVKNRGGSPYHQYDMLSTDALGGHGGPTAESWLPAKSPPSSKIGNKSINASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGPAPSPIVDTDHQLLQDTPTGSNSSLNTSLPSPGAWPFSASENIHSTSALSAKFGDYKSTWSPDPIGHNPSHHSNKMWKNQMSRNSPGLPRPPPGLTNNPKATNSPWNSTAPRSVRGWGGQDSRMASASAWSDGGSVRPSYWLILLNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQSALHMCVLGNTTILAEFATDEEVSRYLAQAQPPAPSAPGAGWQPLEVGPNHPAEPVGPALNLFGGTTGLGQWSSGGGGSDLSGVSLWGTPSSYTSSLWGVPSTEDAHRMGSPAPLLPGDLLGGGTDSI

Target ORF information:

RefSeq Version	XM_018093654.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093654.1

ORF Insert Sequence:

ATGCAAAATG GCTGCCAGTC TCTCGTTTAT GCACATTTCC CTTTAAAGAG GAGAAAACAG 
AAATATATAA ACAAAGAGAA GGAGCAAGAA AGGGAAGAAC AGTTAATGGA AGACAAGAAA 
AGGAAGAAAG AGGATAAAAA GAAGAAAGAA GCTGCTCAGA AGGTCACAGA ACAAAAAACC 
AAAGTGCCCG AAGTGACGAA ACCAAGTTCA AGCCAAGCGA CGGCCGCCAG CCTACCTGGC 
AGCTCCCCCT CGCCACCAGT CAATGGTGGC AACAATGCCA AAAGGGTGGC AGTGCCGAAC 
GGACAACCGC CCAGCACCGC CCGCTATATG CCTCGGGAGG TGCCGCCGCG ATTCCGCTGC 
CAGCAGGACC ACAAAGTGTT ACTGAAGCGT GGACAGCCTC CTCCAGCGTC CTGTATGCTG 
CCGGGGGGAG GAGCAGGGAC TCCCCCCTCA ACCACTGCTC CAGGGGCCGG CCCAGGCAAC 
GCACAGCCAG GGGCGGGGAC ACTGCTGCCA GGGGAAAGCG GGGCTTCAAC AGACTCATCA 
TCGGGGGCAG CTGCTACTTC AAATTATGCA AATTCCACTT GGGGCTCAGG AGGCCCCACC 
AGCAGCGGCC CCACCACAAA CCCTCCCCAC GCCTGGGACA AAGTGATTGT AGACGGGTCG 
GACATGGAAG AGTGGCCTTG CATAGCCGGC GTGGACGCAG AGCCCCCTTC CGACAACGCC 
ACAGACAACA ACAGTGCCTC GAACTCAGCC TCGGAGAAGG GACCCCCACA AGGAAGCACC 
ACTAGCCACA AAGGGCGAGG GGGCCAGGGC CAGCAGCAAC AGCAGCAGCA GCCTGGTAGT 
GAATGTATCC AGGGCGTCTG GAAATCTGAC CCCAAGGCTA AGCCCGTCCC GTCTTCCAAC 
CCTGCTTCAG AGGGTGTAAA TAGACTGGGA AACTGGAGAA ATGGGGGTGG GTCAGAGAGA 
GCAGGTTCTG CCTCTGGTTT CAGCAACTTT AATCCAAATA CTAACCCATC TGCTTGGCCT 
GCCCTTGTCC AGGAAGGCAA CAGGAAAGGG AACCCAGAGT CAGACAGCAG CTTTTCTGGC 
GCACAACTCG GCCCAGTGGG TTTGACCTCT CGGGAGCAGC AGTCAAAGAT GGAAAATGCA 
GGTTCCAGCT TTGCAGGATC GGGCAGGGAG TCTGCCACAA CCCATCACAC TGACGGACCA 
AAAAATGGAA ACACTAACTC TACGAACTCA AATACTTTGA ACCCTCCTGA TAACAAGGGA 
ACAGCTTTTG GGGTGGGCTC AATGGATTCC TGCCGGGGTT CAGACTCCCC TGCTCAAAGC 
ACTGGAGAAA GAAAGAATGG GGGGATTGGC TCTTGGGGAG CGGGAAGGGG TCAGTCTGCC 
ACAGGAGATG CTAATTGTGG ACATAGCAAC TCTGGGAGCA ATGGACGAGA GAGAGAAGAC 
GCAAGGAAAG CGCCTTCATC TCATAGACCC AATGGGTCAA GGGGGGAATC TTGGGAAAGC 
AAAAGTGGGA ATGGTTCTTG GGGTTACAGC CCGCAGGCAG ACCTCAGTGA AGTGAAATGG 
AGTGAAGGGA ACAAATTGGG GGCTGGAGGG GCGTCTCAGG GAGAATGGAA GCAGCCAGCC 
GGGCATGAGG ACTTAAAGGT GGGAGAGTGG GGAGGCTTGA ACCAAGGAAA CTCTAGCACT 
GGAGCATGGG ACAATCAGAA GGGCCACTCT CTATCGGATA ACCAGGGCAA CACGTCATCA 
GCAGCCTGCT GGGGCCGAGC CCCCAGCTCT GCAGGAAGTG AAGCTGGGGG GCAAAGCACT 
GGAAGCAACC CTAAAGCAGG GGGGAGCGGA GGAAGCGGAG ACAGCCTGAA CTCCAGCAGT 
CGACGTTCAC ACAGACCTGC CCTCCCAGAC AGTCAGGCTG CTCTTCAGAC TTTACTGAAT 
CGCACTGATT TGGATCCCCG GGTGCTGTCC AACTCTGGCT GGGGCCAAAC CCAGATCAAG 
CAAGATGCAT CATGGGAGAG TGAGGAAGTG CCCTCTCGAA CTGAAGGCAA ATCAGACCGT 
GGGACTGAAG GTTGGGAGAA CGTGTCCCAA GCCAAAACCT CAGGGGGTTG GGGGGATGCT 
CCCAGCCAAA GCAATCAAAA CAAGTCAGGC TGGGGGGAGC CCCCTGTCCC TGCACCTTCA 
ACCCAAGAAT GGAAGGACAC CAAAGCAACT GGCTGGAATG ACTATAAAAA CAGCCCAACT 
GGTTGGGTTG GTGGAGGAGG TGGTGGGGTG GTTGGAGCAC GACCAGAGGA CAAGGCAGTT 
CCAGGTTGGA ACAATGAAAG TGGTGGGGCT GCGGAGCCTG GGTGGAGTAC TCGCCAGTCT 
AATCCAGGAG GTGGCTGGTC AGCAGGAAAG AATGGTTGGG GGGGAAATGG TAGTGAGGAC 
ATGGAGCCTC CCAAGGCCCC TGGAGGCTGG GAAGGCTCAG GAAGCAAATC CTCCTCTGGC 
TGGGGTGAAG TTGGACAAAA TGATGTGGGT TCTTGGGGTA ATGGGGCAAA CTCAAATCCA 
GCTCCACGAA CTGGCTGGGA GGAGACTAAG AGGCCCCAGG GAGGAGGAAA CTGGGGTGAG 
GCAGCTAGGC CCCAACTACC CTGGAACAAA CAGCAGGAAT CAACGCCATC TTCAGCAACT 
CCTAGCACCT GGGGGGGCTC TCAGGCTCCC CCGCCACCTG GAAATGGGAG GTCACAGCCA 
ACTTCAGGGT GGAAGGGTGG TCCCCAGCCA ACTGTTTCCA AGGAGGAAGA GCCAAGTGGC 
TGGGAGGAAC CATCCTCACA ATCCATTGGT CATAAAATGG ACATTGATGA CGGAACAGCA 
GCTTGGGGGG ATCCTAACAA TTACAATTAC AAAAATGTCA GCCTGTGGGA CAAGAATGCT 
CAAGCAGGCC CTGGGCAGAT GGCTCGGGAG CAAGGAATAT CTGGGACATT GGCAAACAAG 
GCAGCTCCCT CAGTGTGGAG TAAAAACAAA GGACCCCCAA CTGACAATGG GACATCAGCT 
TGGGGTGAGC CAACTGAACC TGCTTCAGGG TGGGGAGAAG GTGAGGAGTC GGGTCCACCA 
ACACCAGGAT GGGGAAGTGC ACCCCAAGTA AATCCCAGTG CCATGAAATC TGGTACAAAA 
TCTATGCAAG ATGGCTGGGG TGAGAGTGAT GGGCCGGTGA CAGGAACACG TCACAGCAGC 
TGGGATGAAG AAGAAGAGGG TGGAGTCTGG AACAGTGCAG GTTCTCAGGG AAGTGGCTCC 
TCCCACAACT CTGCAGGTTG GGGACAAGGA GGAAAGAAAC CACAGATCAA GGGTCCCTTA 
AAAGGAGGGA GCAATGATAC CTGGATGAAC ACCTTGCCCA AACAGTTCTC CAACATGGGA 
TTGTTGAGCC AAGTTGATGA CCCCTCTACC ACTAAGATGG ACCATCCTGG CGGGCTTCCA 
GATAAGAAGT TTGAGACAGA CAAGCGGGGC GTGAATCTCG GGGATTTTAA TGACATGATG 
CGGAAGGAAC GGTCTGGCTT CCGTCTGAAC AATTCCAAAG AGATGGGAGC TGCAGACAGT 
GGGCCTTATT TTGAGAAGCT GACTTTGCCT TTCTCCAATC AAGACGGGTG CCTTGGGGAT 
GAGGCTCCCT GTTCCCCTTT TTCTCCCTCC CCTAGCTACA AGCTATCTCC CTCCGGCTCC 
TCGCTCCCTT CCGTGGGCCT GGGGGCTATC GGTTCTGGCC TCAACCCCCA AACCTTCGCT 
GCTAGACAAG GTGGCAATCA CGGACTATTT GGAAACAGCG GTGCACAATC GCGAGGCATG 
CACAACCCTG TGCCATCGCT CAACTCCTCT CCCAACCTCC GGGCACAAGT GCCTCCCCAG 
TTCCTCTCTC CCCAGGTCTC AGCCTCCATG CTAAAGCAGT TTCCTAACAG TAACATGAAT 
CCAGGACTTT TCAATATGGG GCCACAGCTT TCCCCTCAGC AGATTGCAAT GCTGAGCCAG 
CTTCCTCAAA TTCAGCAGTT CCAGCTGGCA TGTCAGCTCC TCTTGCAACA ACAACAACAG 
CAGCAGCAAC AGCAGCAGCA GCAGCAGCAA CTCCTTCAGA ACCAGAGAAA GATCTCACAA 
GCGGTCAGAC AGCAGCAGGA ACAGCAGATA GCACGCATGG TAAGTGCCCT ACAACAGCAG 
CAACAGCAAC AGCAGCAGCA GCAACAGAGG CAGCCAGGAA TGAAGCACTC TCCCTCTCAC 
CCTGCTGGAC CCAAAGGTCA CTTAGATGCT GTTGGACTCT CAGACATGCA AGCCAAAGGG 
CACATGCCAT ATGGATCTGG TTTTGGCTCA AGTGGGATGG ATTATGGTAC AGTAGGTAAG 
GAGGCGGGAG CAGAGTCTCG ATTTAAACAG TGGACATCCA TGATGGAGGG GCTACCGTCC 
ACTGTTTCTC AAGATACCAG CCTGCATAAA AATGGCTCCA TTGCACCCCC AGTAAAAAAT 
CGTGGAGGTT CCCCCTACCA CCAATATGAC ATGTTGTCTA CGGATGCTTT GGGAGGGCAT 
GGGGGGCCAA CAGCTGAGAG CTGGTTACCT GCCAAATCAC CCCCTTCAAG CAAAATTGGA 
AACAAGTCAA TCAATGCCAG CTGGCCCCCA GAGTTTCAGC CAGGAGTGCC ATGGAAAGGG 
ATCCAAAATA TTGATCCAGA ATCTGATCCT TATGTTACCC CTGGCAGTGT GCTTGGGGGT 
CCAGCCCCAT CTCCCATTGT AGATACTGAC CACCAGCTAC TGCAGGACAC CCCCACAGGG 
TCCAATTCTT CCCTCAACAC CTCGCTGCCT TCACCTGGTG CCTGGCCCTT TAGTGCCTCT 
GAGAATATCC ATAGCACTTC AGCACTCTCT GCAAAGTTTG GGGACTACAA ATCGACCTGG 
TCACCAGACC CCATAGGACA TAACCCATCA CATCACTCAA ACAAGATGTG GAAAAACCAG 
ATGTCCAGGA ACAGCCCTGG GCTGCCTCGC CCACCTCCAG GGCTCACAAA CAACCCCAAA 
GCCACCAACT CTCCCTGGAA CAGCACAGCA CCTCGCTCCG TCAGGGGCTG GGGCGGACAG 
GACTCCAGAA TGGCTTCTGC ATCAGCTTGG AGTGATGGTG GATCTGTGCG CCCCAGCTAC 
TGGCTGATCC TTCTTAATCT CACACCACAG ATCGATGGTT CCACCCTGCG AACGATCTGC 
ATGCAGCATG GCCCCCTGCT GACATTCCAT CTCAACCTTA CCCAGGGCAC AGCTCTAATC 
CGATACAGCA CCAAACAGGA GGCAGCCAAG GCCCAGAGTG CCCTCCATAT GTGTGTTCTG 
GGTAATACAA CTATCCTGGC GGAGTTTGCA ACGGATGAGG AGGTGAGCCG GTATCTTGCA 
CAAGCTCAGC CACCAGCTCC CTCTGCTCCA GGAGCAGGCT GGCAGCCTTT GGAAGTGGGA 
CCGAACCACC CAGCTGAACC AGTAGGGCCA GCCCTAAACC TTTTTGGAGG AACCACAGGG 
CTAGGGCAAT GGAGCAGCGG CGGTGGTGGT AGTGACCTGT CAGGGGTTTC TCTCTGGGGT 
ACTCCTAGCA GCTACACATC CAGTCTGTGG GGTGTGCCGA GCACAGAGGA CGCCCACAGG 
ATGGGCAGCC CTGCCCCGTT ACTACCCGGT GACCTTCTGG GAGGAGGGAC CGACTCGATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnrc6b ( XM_018093655.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949144.1 Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X7, mRNA.

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CloneID	OXa44223
Clone ID Related Accession (Same CDS sequence)	XM_018093655.1
Accession Version	XM_018093655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trinucleotide repeat-containing gene 6B protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAATG GCTGCCAGTC TCTCGTTTAT GCACATTTCC CTTTAAAGAG GAGAAAACAG 
AAATATATAA ACAAAGAGAA GGAGCAAGAA AGGGAAGAAC AGTTAATGGA AGACAAGAAA 
AGGAAGAAAG AGGATAAAAA GAAGAAAGAA GCTGCTCAGA AGGTCACAGA ACAAAAAACC 
AAAGTGCCCG AAGTGACGAA ACCAAGTTCA AGCCAAGCGA CGGCCGCCAG CCTACCTGGC 
AGCTCCCCCT CGCCACCAGT CAATGGTGGC AACAATGCCA AAAGGGTGGC AGTGCCGAAC 
GGACAACCGC CCAGCACCGC CCGCTATATG CCTCGGGAGG TGCCGCCGCG ATTCCGCTGC 
CAGCAGGACC ACAAAGTGTT ACTGAAGCGT GGACAGCCTC CTCCAGCGTC CTGTATGCTG 
CCGGGGGGAG GAGCAGGGAC TCCCCCCTCA ACCACTGCTC CAGGGGCCGG CCCAGGCAAC 
GCACAGCCAG GGGCGGGGAC ACTGCTGCCA GGGGAAAGCG GGGCTTCAAC AGACTCATCA 
TCGGGGGCAG CTGCTACTTC AAATTATGCA AATTCCACTT GGGGCTCAGG AGGCCCCACC 
AGCAGCGGCC CCACCACAAA CCCTCCCCAC GCCTGGGACA AAGTGATTGT AGACGGGTCG 
GACATGGAAG AGTGGCCTTG CATAGCCGGC GTGGACGCAG AGCCCCCTTC CGACAACGCC 
ACAGACAACA ACAGTGCCTC GAACTCAGCC TCGGAGAAGG GACCCCCACA AGGAAGCACC 
ACTAGCCACA AAGGGCGAGG GGGCCAGGGC CAGCAGCAAC AGCAGCAGCA GCCTGGTAGT 
GAATGTATCC AGGGCGTCTG GAAATCTGAC CCCAAGGCTA AGCCCGTCCC GTCTTCCAAC 
CCTGCTTCAG AGGGTGTAAA TAGACTGGGA AACTGGAGAA ATGGGGGTGG GTCAGAGAGA 
GCAGGTTCTG CCTCTGGTTT CAGCAACTTT AATCCAAATA CTAACCCATC TGCTTGGCCT 
GCCCTTGTCC AGGAAGGCAA CAGGAAAGGG AACCCAGAGT CAGACAGCAG CTTTTCTGGC 
GCACAACTCG GCCCAGTGGG TTTGACCTCT CGGGAGCAGC AGTCAAAGAT GGAAAATGCA 
GGTTCCAGCT TTGCAGGATC GGGCAGGGAG TCTGCCACAA CCCATCACAC TGACGGACCA 
AAAAATGGAA ACACTAACTC TACGAACTCA AATACTTTGA ACCCTCCTGA TAACAAGGGA 
ACAGCTTTTG GGGTGGGCTC AATGGATTCC TGCCGGGGTT CAGACTCCCC TGCTCAAAGC 
ACTGGAGAAA GAAAGAATGG GGGGATTGGC TCTTGGGGAG CGGGAAGGGG TCAGTCTGCC 
ACAGGAGATG CTAATTGTGG ACATAGCAAC TCTGGGAGCA ATGGACGAGA GAGAGAAGAC 
GCAAGGAAAG CGCCTTCATC TCATAGACCC AATGGGTCAA GGGGGGAATC TTGGGAAAGC 
AAAAGTGGGA ATGGTTCTTG GGGTTACAGC CCGCAGGCAG ACCTCAGTGA AGTGAAATGG 
AGTGAAGGGA ACAAATTGGG GGCTGGAGGG GCGTCTCAGG GAGAATGGAA GCAGCCAGCC 
GGGCATGAGG ACTTAAAGGT GGGAGAGTGG GGAGGCTTGA ACCAAGGAAA CTCTAGCACT 
GGAGCATGGG ACAATCAGAA GGGCCACTCT CTATCGGATA ACCAGGGCAA CACGTCATCA 
GCAGCCTGCT GGGGCCGAGC CCCCAGCTCT GCAGGAAGTG AAGCTGGGGG GCAAAGCACT 
GGAAGCAACC CTAAAGCAGG GGGGAGCGGA GGAAGCGGAG ACAGCCTGAA CTCCAGCAGT 
CGACGTTCAC ACAGACCTGC CCTCCCAGAC AGTCAGGCTG CTCTTCAGAC TTTACTGAAT 
CGCACTGATT TGGATCCCCG GGTGCTGTCC AACTCTGGCT GGGGCCAAAC CCAGATCAAG 
CAAGATGCAT CATGGGAGAG TGAGGAAGTG CCCTCTCGAA CTGAAGGCAA ATCAGACCGT 
GGGACTGAAG GTTGGGAGAA CGTGTCCCAA GCCAAAACCT CAGGGGGTTG GGGGGATGCT 
CCCAGCCAAA GCAATCAAAA CAAGTCAGGC TGGGGGGAGC CCCCTGTCCC TGCACCTTCA 
ACCCAAGAAT GGAAGGACAC CAAAGCAACT GGCTGGAATG ACTATAAAAA CAGCCCAACT 
GGTTGGGTTG GTGGAGGAGG TGGTGGGGTG GTTGGAGCAC GACCAGAGGA CAAGGCAGTT 
CCAGGTTGGA ACAATGAAAG TGGTGGGGCT GCGGAGCCTG GGTGGAGTAC TCGCCAGTCT 
AATCCAGGAG GTGGCTGGTC AGCAGGAAAG AATGGTTGGG GGGGAAATGG TAGTGAGGAC 
ATGGAGCCTC CCAAGGCCCC TGGAGGCTGG GAAGGCTCAG GAAGCAAATC CTCCTCTGGC 
TGGGGTGAAG TTGGACAAAA TGATGTGGGT TCTTGGGGTA ATGGGGCAAA CTCAAATCCA 
GCTCCACGAA CTGGCTGGGA GGAGACTAAG AGGCCCCAGG GAGGAGGAAA CTGGGGTGAG 
GCAGCTAGGC CCCAACTACC CTGGAACAAA CAGCAGGAAT CAACGCCATC TTCAGCAACT 
CCTAGCACCT GGGGGGGCTC TCAGGCTCCC CCGCCACCTG GAAATGGGAG GTCACAGCCA 
ACTTCAGGGT GGAAGGGTGG TCCCCAGCCA ACTGTTTCCA AGGAGGAAGA GCCAAGTGGC 
TGGGAGGAAC CATCCTCACA ATCCATTGGT CATAAAATGG ACATTGATGA CGGAACAGCA 
GCTTGGGGGG ATCCTAACAA TTACAATTAC AAAAATGTCA GCCTGTGGGA CAAGAATGCT 
CAAGCAGGCC CTGGGCAGAT GGCTCGGGAG CAAGGAATAT CTGGGACATT GGCAAACAAG 
GCAGCTCCCT CAGTGTGGAG TAAAAACAAA GGACCCCCAA CTGACAATGG GACATCAGCT 
TGGGGTGAGC CAACTGAACC TGCTTCAGGG TGGGGAGAAG GTGAGGAGTC GGGTCCACCA 
ACACCAGGAT GGGGAAGTGC ACCCCAAGTA AATCCCAGTG CCATGAAATC TGGTACAAAA 
TCTATGCAAG ATGGCTGGGG TGAGAGTGAT GGGCCGGTGA CAGGAACACG TCACAGCAGC 
TGGGATGAAG AAGAAGAGGG TGGAGTCTGG AACAGTGCAG GTTCTCAGGG AAGTGGCTCC 
TCCCACAACT CTGCAGGTTG GGGACAAGGA GGAAAGAAAC CACAGATCAA GGGTCCCTTA 
AAAGGAGGGA GCAATGATAC CTGGATGAAC ACCTTGCCCA AACAGTTCTC CAACATGGGA 
TTGTTGAGCC AAGTTGATGA CCCCTCTACC ACTAAGATGG ACCATCCTGG CGGGCTTCCA 
GATAAGAAGT TTGAGACAGA CAAGCGGGGC GTGAATCTCG GGGATTTTAA TGACATGATG 
CGGAAGGAAC GGTCTGGCTT CCGTCTGAAC AATTCCAAAG AGATGGGAGC TGCAGACAGT 
GGGCCTTATT TTGAGAAGGG TGGCAATCAC GGACTATTTG GAAACAGCGG TGCACAATCG 
CGAGGCATGC ACAACCCTGT GCCATCGCTC AACTCCTCTC CCAACCTCCG GGCACAAGTG 
CCTCCCCAGT TCCTCTCTCC CCAGGTCTCA GCCTCCATGC TAAAGCAGTT TCCTAACAGT 
AACATGAATC CAGGACTTTT CAATATGGGG CCACAGCTTT CCCCTCAGCA GATTGCAATG 
CTGAGCCAGC TTCCTCAAAT TCAGCAGTTC CAGCTGGCAT GTCAGCTCCT CTTGCAACAA 
CAACAACAGC AGCAGCAACA GCAGCAGCAG CAGCAGCAAC TCCTTCAGAA CCAGAGAAAG 
ATCTCACAAG CGGTCAGACA GCAGCAGGAA CAGCAGCAGA TAGCACGCAT GGTAAGTGCC 
CTACAACAGC AGCAACAGCA ACAGCAGCAG CAGCAACAGA GGCAGCCAGG AATGAAGCAC 
TCTCCCTCTC ACCCTGCTGG ACCCAAAGGT CACTTAGATG CTGTTGGACT CTCAGACATG 
CAAGCCAAAG GGCACATGCC ATATGGATCT GGTTTTGGCT CAAGTGGGAT GGATTATGGT 
ACAGTAGGTA AGGAGGCGGG AGCAGAGTCT CGATTTAAAC AGTGGACATC CATGATGGAG 
GGGCTACCGT CCACTGTTTC TCAAGATACC AGCCTGCATA AAAATGGCTC CATTGCACCC 
CCAGTAAAAA ATCGTGGAGG TTCCCCCTAC CACCAATATG ACATGTTGTC TACGGATGCT 
TTGGGAGGGC ATGGGGGGCC AACAGCTGAG AGCTGGTTAC CTGCCAAATC ACCCCCTTCA 
AGCAAAATTG GAAACAAGTC AATCAATGCC AGCTGGCCCC CAGAGTTTCA GCCAGGAGTG 
CCATGGAAAG GGATCCAAAA TATTGATCCA GAATCTGATC CTTATGTTAC CCCTGGCAGT 
GTGCTTGGGG GTCCAGCCCC ATCTCCCATT GTAGATACTG ACCACCAGCT ACTGCAGGAC 
ACCCCCACAG GGTCCAATTC TTCCCTCAAC ACCTCGCTGC CTTCACCTGG TGCCTGGCCC 
TTTAGTGCCT CTGAGAATAT CCATAGCACT TCAGCACTCT CTGCAAAGTT TGGGGACTAC 
AAATCGACCT GGTCACCAGA CCCCATAGGA CATAACCCAT CACATCACTC AAACAAGATG 
TGGAAAAACC AGATGTCCAG GAACAGCCCT GGGCTGCCTC GCCCACCTCC AGGGCTCACA 
AACAACCCCA AAGCCACCAA CTCTCCCTGG AACAGCACAG CACCTCGCTC CGTCAGGGGC 
TGGGGCGGAC AGGACTCCAG AATGGCTTCT GCATCAGCTT GGAGTGATGG TGGATCTGTG 
CGCCCCAGCT ACTGGCTGAT CCTTCTTAAT CTCACACCAC AGATCGATGG TTCCACCCTG 
CGAACGATCT GCATGCAGCA TGGCCCCCTG CTGACATTCC ATCTCAACCT TACCCAGGGC 
ACAGCTCTAA TCCGATACAG CACCAAACAG GAGGCAGCCA AGGCCCAGAG TGCCCTCCAT 
ATGTGTGTTC TGGGTAATAC AACTATCCTG GCGGAGTTTG CAACGGATGA GGAGGTGAGC 
CGGTATCTTG CACAAGCTCA GCCACCAGCT CCCTCTGCTC CAGGAGCAGG CTGGCAGCCT 
TTGGAAGTGG GACCGAACCA CCCAGCTGAA CCAGTAGGGC CAGCCCTAAA CCTTTTTGGA 
GGAACCACAG GGCTAGGGCA ATGGAGCAGC GGCGGTGGTG GTAGTGACCT GTCAGGGGTT 
TCTCTCTGGG GTACTCCTAG CAGCTACACA TCCAGTCTGT GGGGTGTGCC GAGCACAGAG 
GACGCCCACA GGATGGGCAG CCCTGCCCCG TTACTACCCG GTGACCTTCT GGGAGGAGGG 
ACCGACTCGA TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949144.1
CDS	49..5523
Translation	MQNGCQSLVYAHFPLKRRKQKYINKEKEQEREEQLMEDKKRKKEDKKKKEAAQKVTEQKTKVPEVTKPSSSQATAASLPGSSPSPPVNGGNNAKRVAVPNGQPPSTARYMPREVPPRFRCQQDHKVLLKRGQPPPASCMLPGGGAGTPPSTTAPGAGPGNAQPGAGTLLPGESGASTDSSSGAAATSNYANSTWGSGGPTSSGPTTNPPHAWDKVIVDGSDMEEWPCIAGVDAEPPSDNATDNNSASNSASEKGPPQGSTTSHKGRGGQGQQQQQQQPGSECIQGVWKSDPKAKPVPSSNPASEGVNRLGNWRNGGGSERAGSASGFSNFNPNTNPSAWPALVQEGNRKGNPESDSSFSGAQLGPVGLTSREQQSKMENAGSSFAGSGRESATTHHTDGPKNGNTNSTNSNTLNPPDNKGTAFGVGSMDSCRGSDSPAQSTGERKNGGIGSWGAGRGQSATGDANCGHSNSGSNGREREDARKAPSSHRPNGSRGESWESKSGNGSWGYSPQADLSEVKWSEGNKLGAGGASQGEWKQPAGHEDLKVGEWGGLNQGNSSTGAWDNQKGHSLSDNQGNTSSAACWGRAPSSAGSEAGGQSTGSNPKAGGSGGSGDSLNSSSRRSHRPALPDSQAALQTLLNRTDLDPRVLSNSGWGQTQIKQDASWESEEVPSRTEGKSDRGTEGWENVSQAKTSGGWGDAPSQSNQNKSGWGEPPVPAPSTQEWKDTKATGWNDYKNSPTGWVGGGGGGVVGARPEDKAVPGWNNESGGAAEPGWSTRQSNPGGGWSAGKNGWGGNGSEDMEPPKAPGGWEGSGSKSSSGWGEVGQNDVGSWGNGANSNPAPRTGWEETKRPQGGGNWGEAARPQLPWNKQQESTPSSATPSTWGGSQAPPPPGNGRSQPTSGWKGGPQPTVSKEEEPSGWEEPSSQSIGHKMDIDDGTAAWGDPNNYNYKNVSLWDKNAQAGPGQMAREQGISGTLANKAAPSVWSKNKGPPTDNGTSAWGEPTEPASGWGEGEESGPPTPGWGSAPQVNPSAMKSGTKSMQDGWGESDGPVTGTRHSSWDEEEEGGVWNSAGSQGSGSSHNSAGWGQGGKKPQIKGPLKGGSNDTWMNTLPKQFSNMGLLSQVDDPSTTKMDHPGGLPDKKFETDKRGVNLGDFNDMMRKERSGFRLNNSKEMGAADSGPYFEKGGNHGLFGNSGAQSRGMHNPVPSLNSSPNLRAQVPPQFLSPQVSASMLKQFPNSNMNPGLFNMGPQLSPQQIAMLSQLPQIQQFQLACQLLLQQQQQQQQQQQQQQQLLQNQRKISQAVRQQQEQQQIARMVSALQQQQQQQQQQQQRQPGMKHSPSHPAGPKGHLDAVGLSDMQAKGHMPYGSGFGSSGMDYGTVGKEAGAESRFKQWTSMMEGLPSTVSQDTSLHKNGSIAPPVKNRGGSPYHQYDMLSTDALGGHGGPTAESWLPAKSPPSSKIGNKSINASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGPAPSPIVDTDHQLLQDTPTGSNSSLNTSLPSPGAWPFSASENIHSTSALSAKFGDYKSTWSPDPIGHNPSHHSNKMWKNQMSRNSPGLPRPPPGLTNNPKATNSPWNSTAPRSVRGWGGQDSRMASASAWSDGGSVRPSYWLILLNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQSALHMCVLGNTTILAEFATDEEVSRYLAQAQPPAPSAPGAGWQPLEVGPNHPAEPVGPALNLFGGTTGLGQWSSGGGGSDLSGVSLWGTPSSYTSSLWGVPSTEDAHRMGSPAPLLPGDLLGGGTDSI

Target ORF information:

RefSeq Version	XM_018093655.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093655.1

ORF Insert Sequence:

ATGCAAAATG GCTGCCAGTC TCTCGTTTAT GCACATTTCC CTTTAAAGAG GAGAAAACAG 
AAATATATAA ACAAAGAGAA GGAGCAAGAA AGGGAAGAAC AGTTAATGGA AGACAAGAAA 
AGGAAGAAAG AGGATAAAAA GAAGAAAGAA GCTGCTCAGA AGGTCACAGA ACAAAAAACC 
AAAGTGCCCG AAGTGACGAA ACCAAGTTCA AGCCAAGCGA CGGCCGCCAG CCTACCTGGC 
AGCTCCCCCT CGCCACCAGT CAATGGTGGC AACAATGCCA AAAGGGTGGC AGTGCCGAAC 
GGACAACCGC CCAGCACCGC CCGCTATATG CCTCGGGAGG TGCCGCCGCG ATTCCGCTGC 
CAGCAGGACC ACAAAGTGTT ACTGAAGCGT GGACAGCCTC CTCCAGCGTC CTGTATGCTG 
CCGGGGGGAG GAGCAGGGAC TCCCCCCTCA ACCACTGCTC CAGGGGCCGG CCCAGGCAAC 
GCACAGCCAG GGGCGGGGAC ACTGCTGCCA GGGGAAAGCG GGGCTTCAAC AGACTCATCA 
TCGGGGGCAG CTGCTACTTC AAATTATGCA AATTCCACTT GGGGCTCAGG AGGCCCCACC 
AGCAGCGGCC CCACCACAAA CCCTCCCCAC GCCTGGGACA AAGTGATTGT AGACGGGTCG 
GACATGGAAG AGTGGCCTTG CATAGCCGGC GTGGACGCAG AGCCCCCTTC CGACAACGCC 
ACAGACAACA ACAGTGCCTC GAACTCAGCC TCGGAGAAGG GACCCCCACA AGGAAGCACC 
ACTAGCCACA AAGGGCGAGG GGGCCAGGGC CAGCAGCAAC AGCAGCAGCA GCCTGGTAGT 
GAATGTATCC AGGGCGTCTG GAAATCTGAC CCCAAGGCTA AGCCCGTCCC GTCTTCCAAC 
CCTGCTTCAG AGGGTGTAAA TAGACTGGGA AACTGGAGAA ATGGGGGTGG GTCAGAGAGA 
GCAGGTTCTG CCTCTGGTTT CAGCAACTTT AATCCAAATA CTAACCCATC TGCTTGGCCT 
GCCCTTGTCC AGGAAGGCAA CAGGAAAGGG AACCCAGAGT CAGACAGCAG CTTTTCTGGC 
GCACAACTCG GCCCAGTGGG TTTGACCTCT CGGGAGCAGC AGTCAAAGAT GGAAAATGCA 
GGTTCCAGCT TTGCAGGATC GGGCAGGGAG TCTGCCACAA CCCATCACAC TGACGGACCA 
AAAAATGGAA ACACTAACTC TACGAACTCA AATACTTTGA ACCCTCCTGA TAACAAGGGA 
ACAGCTTTTG GGGTGGGCTC AATGGATTCC TGCCGGGGTT CAGACTCCCC TGCTCAAAGC 
ACTGGAGAAA GAAAGAATGG GGGGATTGGC TCTTGGGGAG CGGGAAGGGG TCAGTCTGCC 
ACAGGAGATG CTAATTGTGG ACATAGCAAC TCTGGGAGCA ATGGACGAGA GAGAGAAGAC 
GCAAGGAAAG CGCCTTCATC TCATAGACCC AATGGGTCAA GGGGGGAATC TTGGGAAAGC 
AAAAGTGGGA ATGGTTCTTG GGGTTACAGC CCGCAGGCAG ACCTCAGTGA AGTGAAATGG 
AGTGAAGGGA ACAAATTGGG GGCTGGAGGG GCGTCTCAGG GAGAATGGAA GCAGCCAGCC 
GGGCATGAGG ACTTAAAGGT GGGAGAGTGG GGAGGCTTGA ACCAAGGAAA CTCTAGCACT 
GGAGCATGGG ACAATCAGAA GGGCCACTCT CTATCGGATA ACCAGGGCAA CACGTCATCA 
GCAGCCTGCT GGGGCCGAGC CCCCAGCTCT GCAGGAAGTG AAGCTGGGGG GCAAAGCACT 
GGAAGCAACC CTAAAGCAGG GGGGAGCGGA GGAAGCGGAG ACAGCCTGAA CTCCAGCAGT 
CGACGTTCAC ACAGACCTGC CCTCCCAGAC AGTCAGGCTG CTCTTCAGAC TTTACTGAAT 
CGCACTGATT TGGATCCCCG GGTGCTGTCC AACTCTGGCT GGGGCCAAAC CCAGATCAAG 
CAAGATGCAT CATGGGAGAG TGAGGAAGTG CCCTCTCGAA CTGAAGGCAA ATCAGACCGT 
GGGACTGAAG GTTGGGAGAA CGTGTCCCAA GCCAAAACCT CAGGGGGTTG GGGGGATGCT 
CCCAGCCAAA GCAATCAAAA CAAGTCAGGC TGGGGGGAGC CCCCTGTCCC TGCACCTTCA 
ACCCAAGAAT GGAAGGACAC CAAAGCAACT GGCTGGAATG ACTATAAAAA CAGCCCAACT 
GGTTGGGTTG GTGGAGGAGG TGGTGGGGTG GTTGGAGCAC GACCAGAGGA CAAGGCAGTT 
CCAGGTTGGA ACAATGAAAG TGGTGGGGCT GCGGAGCCTG GGTGGAGTAC TCGCCAGTCT 
AATCCAGGAG GTGGCTGGTC AGCAGGAAAG AATGGTTGGG GGGGAAATGG TAGTGAGGAC 
ATGGAGCCTC CCAAGGCCCC TGGAGGCTGG GAAGGCTCAG GAAGCAAATC CTCCTCTGGC 
TGGGGTGAAG TTGGACAAAA TGATGTGGGT TCTTGGGGTA ATGGGGCAAA CTCAAATCCA 
GCTCCACGAA CTGGCTGGGA GGAGACTAAG AGGCCCCAGG GAGGAGGAAA CTGGGGTGAG 
GCAGCTAGGC CCCAACTACC CTGGAACAAA CAGCAGGAAT CAACGCCATC TTCAGCAACT 
CCTAGCACCT GGGGGGGCTC TCAGGCTCCC CCGCCACCTG GAAATGGGAG GTCACAGCCA 
ACTTCAGGGT GGAAGGGTGG TCCCCAGCCA ACTGTTTCCA AGGAGGAAGA GCCAAGTGGC 
TGGGAGGAAC CATCCTCACA ATCCATTGGT CATAAAATGG ACATTGATGA CGGAACAGCA 
GCTTGGGGGG ATCCTAACAA TTACAATTAC AAAAATGTCA GCCTGTGGGA CAAGAATGCT 
CAAGCAGGCC CTGGGCAGAT GGCTCGGGAG CAAGGAATAT CTGGGACATT GGCAAACAAG 
GCAGCTCCCT CAGTGTGGAG TAAAAACAAA GGACCCCCAA CTGACAATGG GACATCAGCT 
TGGGGTGAGC CAACTGAACC TGCTTCAGGG TGGGGAGAAG GTGAGGAGTC GGGTCCACCA 
ACACCAGGAT GGGGAAGTGC ACCCCAAGTA AATCCCAGTG CCATGAAATC TGGTACAAAA 
TCTATGCAAG ATGGCTGGGG TGAGAGTGAT GGGCCGGTGA CAGGAACACG TCACAGCAGC 
TGGGATGAAG AAGAAGAGGG TGGAGTCTGG AACAGTGCAG GTTCTCAGGG AAGTGGCTCC 
TCCCACAACT CTGCAGGTTG GGGACAAGGA GGAAAGAAAC CACAGATCAA GGGTCCCTTA 
AAAGGAGGGA GCAATGATAC CTGGATGAAC ACCTTGCCCA AACAGTTCTC CAACATGGGA 
TTGTTGAGCC AAGTTGATGA CCCCTCTACC ACTAAGATGG ACCATCCTGG CGGGCTTCCA 
GATAAGAAGT TTGAGACAGA CAAGCGGGGC GTGAATCTCG GGGATTTTAA TGACATGATG 
CGGAAGGAAC GGTCTGGCTT CCGTCTGAAC AATTCCAAAG AGATGGGAGC TGCAGACAGT 
GGGCCTTATT TTGAGAAGGG TGGCAATCAC GGACTATTTG GAAACAGCGG TGCACAATCG 
CGAGGCATGC ACAACCCTGT GCCATCGCTC AACTCCTCTC CCAACCTCCG GGCACAAGTG 
CCTCCCCAGT TCCTCTCTCC CCAGGTCTCA GCCTCCATGC TAAAGCAGTT TCCTAACAGT 
AACATGAATC CAGGACTTTT CAATATGGGG CCACAGCTTT CCCCTCAGCA GATTGCAATG 
CTGAGCCAGC TTCCTCAAAT TCAGCAGTTC CAGCTGGCAT GTCAGCTCCT CTTGCAACAA 
CAACAACAGC AGCAGCAACA GCAGCAGCAG CAGCAGCAAC TCCTTCAGAA CCAGAGAAAG 
ATCTCACAAG CGGTCAGACA GCAGCAGGAA CAGCAGCAGA TAGCACGCAT GGTAAGTGCC 
CTACAACAGC AGCAACAGCA ACAGCAGCAG CAGCAACAGA GGCAGCCAGG AATGAAGCAC 
TCTCCCTCTC ACCCTGCTGG ACCCAAAGGT CACTTAGATG CTGTTGGACT CTCAGACATG 
CAAGCCAAAG GGCACATGCC ATATGGATCT GGTTTTGGCT CAAGTGGGAT GGATTATGGT 
ACAGTAGGTA AGGAGGCGGG AGCAGAGTCT CGATTTAAAC AGTGGACATC CATGATGGAG 
GGGCTACCGT CCACTGTTTC TCAAGATACC AGCCTGCATA AAAATGGCTC CATTGCACCC 
CCAGTAAAAA ATCGTGGAGG TTCCCCCTAC CACCAATATG ACATGTTGTC TACGGATGCT 
TTGGGAGGGC ATGGGGGGCC AACAGCTGAG AGCTGGTTAC CTGCCAAATC ACCCCCTTCA 
AGCAAAATTG GAAACAAGTC AATCAATGCC AGCTGGCCCC CAGAGTTTCA GCCAGGAGTG 
CCATGGAAAG GGATCCAAAA TATTGATCCA GAATCTGATC CTTATGTTAC CCCTGGCAGT 
GTGCTTGGGG GTCCAGCCCC ATCTCCCATT GTAGATACTG ACCACCAGCT ACTGCAGGAC 
ACCCCCACAG GGTCCAATTC TTCCCTCAAC ACCTCGCTGC CTTCACCTGG TGCCTGGCCC 
TTTAGTGCCT CTGAGAATAT CCATAGCACT TCAGCACTCT CTGCAAAGTT TGGGGACTAC 
AAATCGACCT GGTCACCAGA CCCCATAGGA CATAACCCAT CACATCACTC AAACAAGATG 
TGGAAAAACC AGATGTCCAG GAACAGCCCT GGGCTGCCTC GCCCACCTCC AGGGCTCACA 
AACAACCCCA AAGCCACCAA CTCTCCCTGG AACAGCACAG CACCTCGCTC CGTCAGGGGC 
TGGGGCGGAC AGGACTCCAG AATGGCTTCT GCATCAGCTT GGAGTGATGG TGGATCTGTG 
CGCCCCAGCT ACTGGCTGAT CCTTCTTAAT CTCACACCAC AGATCGATGG TTCCACCCTG 
CGAACGATCT GCATGCAGCA TGGCCCCCTG CTGACATTCC ATCTCAACCT TACCCAGGGC 
ACAGCTCTAA TCCGATACAG CACCAAACAG GAGGCAGCCA AGGCCCAGAG TGCCCTCCAT 
ATGTGTGTTC TGGGTAATAC AACTATCCTG GCGGAGTTTG CAACGGATGA GGAGGTGAGC 
CGGTATCTTG CACAAGCTCA GCCACCAGCT CCCTCTGCTC CAGGAGCAGG CTGGCAGCCT 
TTGGAAGTGG GACCGAACCA CCCAGCTGAA CCAGTAGGGC CAGCCCTAAA CCTTTTTGGA 
GGAACCACAG GGCTAGGGCA ATGGAGCAGC GGCGGTGGTG GTAGTGACCT GTCAGGGGTT 
TCTCTCTGGG GTACTCCTAG CAGCTACACA TCCAGTCTGT GGGGTGTGCC GAGCACAGAG 
GACGCCCACA GGATGGGCAG CCCTGCCCCG TTACTACCCG GTGACCTTCT GGGAGGAGGG 
ACCGACTCGA TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnrc6b ( XM_018093656.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949145.1 Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X8, mRNA.

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CloneID	OXa44224
Clone ID Related Accession (Same CDS sequence)	XM_018093656.1
Accession Version	XM_018093656.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trinucleotide repeat-containing gene 6B protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAATG GCTGCCAGTC TCTCGTTTAT GCACATTTCC CTTTAAAGAG GAGAAAACAG 
AAATATATAA ACAAAGAGAA GGAGCAAGAA AGGGAAGAAC AGTTAATGGA AGACAAGAAA 
AGGAAGAAAG AGGATAAAAA GAAGAAAGAA GCTGCTCAGA AGGTCACAGA ACAAAAAACC 
AAAGTGCCCG AAGTGACGAA ACCAAGTTCA AGCCAAGCGA CGGCCGCCAG CCTACCTGGC 
AGCTCCCCCT CGCCACCAGT CAATGGTGGC AACAATGCCA AAAGGGTGGC AGTGCCGAAC 
GGACAACCGC CCAGCACCGC CCGCTATATG CCTCGGGAGG TGCCGCCGCG ATTCCGCTGC 
CAGCAGGACC ACAAAGTGTT ACTGAAGCGT GGACAGCCTC CTCCAGCGTC CTGTATGCTG 
CCGGGGGGAG GAGCAGGGAC TCCCCCCTCA ACCACTGCTC CAGGGGCCGG CCCAGGCAAC 
GCACAGCCAG GGGCGGGGAC ACTGCTGCCA GGGGAAAGCG GGGCTTCAAC AGACTCATCA 
TCGGGGGCAG CTGCTACTTC AAATTATGCA AATTCCACTT GGGGCTCAGG AGGCCCCACC 
AGCAGCGGCC CCACCACAAA CCCTCCCCAC GCCTGGGACA AAGTGATTGT AGACGGGTCG 
GACATGGAAG AGTGGCCTTG CATAGCCGGC GTGGACGCAG AGCCCCCTTC CGACAACGCC 
ACAGACAACA ACAGTGCCTC GAACTCAGCC TCGGAGAAGG GACCCCCACA AGGAAGCACC 
ACTAGCCACA AAGGGCGAGG GGGCCAGGGC CAGCAGCAAC AGCAGCAGCA GCCTGGTAGT 
GAATGTATCC AGGGCGTCTG GAAATCTGAC CCCAAGGCTA AGCCCGTCCC GTCTTCCAAC 
CCTGCTTCAG AGGGTGTAAA TAGACTGGGA AACTGGAGAA ATGGGGGTGG GTCAGAGAGA 
GCAGGTTCTG CCTCTGGTTT CAGCAACTTT AATCCAAATA CTAACCCATC TGCTTGGCCT 
GCCCTTGTCC AGGAAGGCAA CAGGAAAGGG AACCCAGAGT CAGACAGCAG CTTTTCTGGC 
GCACAACTCG GCCCAGTGGG TTTGACCTCT CGGGAGCAGC AGTCAAAGAT GGAAAATGCA 
GGTTCCAGCT TTGCAGGATC GGGCAGGGAG TCTGCCACAA CCCATCACAC TGACGGACCA 
AAAAATGGAA ACACTAACTC TACGAACTCA AATACTTTGA ACCCTCCTGA TAACAAGGGA 
ACAGCTTTTG GGGTGGGCTC AATGGATTCC TGCCGGGGTT CAGACTCCCC TGCTCAAAGC 
ACTGGAGAAA GAAAGAATGG GGGGATTGGC TCTTGGGGAG CGGGAAGGGG TCAGTCTGCC 
ACAGGAGATG CTAATTGTGG ACATAGCAAC TCTGGGAGCA ATGGACGAGA GAGAGAAGAC 
GCAAGGAAAG CGCCTTCATC TCATAGACCC AATGGGTCAA GGGGGGAATC TTGGGAAAGC 
AAAAGTGGGA ATGGTTCTTG GGGTTACAGC CCGCAGGCAG ACCTCAGTGA AGTGAAATGG 
AGTGAAGGGA ACAAATTGGG GGCTGGAGGG GCGTCTCAGG GAGAATGGAA GCAGCCAGCC 
GGGCATGAGG ACTTAAAGGT GGGAGAGTGG GGAGGCTTGA ACCAAGGAAA CTCTAGCACT 
GGAGCATGGG ACAATCAGAA GGGCCACTCT CTATCGGATA ACCAGGGCAA CACGTCATCA 
GCAGCCTGCT GGGGCCGAGC CCCCAGCTCT GCAGGAAGTG AAGCTGGGGG GCAAAGCACT 
GGAAGCAACC CTAAAGCAGG GGGGAGCGGA GGAAGCGGAG ACAGCCTGAA CTCCAGCAGT 
CGACGTTCAC ACAGACCTGC CCTCCCAGAC AGTCAGGCTG CTCTTCAGAC TTTACTGAAT 
CGCACTGATT TGGATCCCCG GGTGCTGTCC AACTCTGGCT GGGGCCAAAC CCAGATCAAG 
CAAGATGCAT CATGGGAGAG TGAGGAAGTG CCCTCTCGAA CTGAAGGCAA ATCAGACCGT 
GGGACTGAAG GTTGGGAGAA CGTGTCCCAA GCCAAAACCT CAGGGGGTTG GGGGGATGCT 
CCCAGCCAAA GCAATCAAAA CAAGTCAGGC TGGGGGGAGC CCCCTGTCCC TGCACCTTCA 
ACCCAAGAAT GGAAGGACAC CAAAGCAACT GGCTGGAATG ACTATAAAAA CAGCCCAACT 
GGTTGGGTTG GTGGAGGAGG TGGTGGGGTG GTTGGAGCAC GACCAGAGGA CAAGGCAGTT 
CCAGGTTGGA ACAATGAAAG TGGTGGGGCT GCGGAGCCTG GGTGGAGTAC TCGCCAGTCT 
AATCCAGGAG GTGGCTGGTC AGCAGGAAAG AATGGTTGGG GGGGAAATGG TAGTGAGGAC 
ATGGAGCCTC CCAAGGCCCC TGGAGGCTGG GAAGGCTCAG GAAGCAAATC CTCCTCTGGC 
TGGGGTGAAG TTGGACAAAA TGATGTGGGT TCTTGGGGTA ATGGGGCAAA CTCAAATCCA 
GCTCCACGAA CTGGCTGGGA GGAGACTAAG AGGCCCCAGG GAGGAGGAAA CTGGGGTGAG 
GCAGCTAGGC CCCAACTACC CTGGAACAAA CAGCAGGAAT CAACGCCATC TTCAGCAACT 
CCTAGCACCT GGGGGGGCTC TCAGGCTCCC CCGCCACCTG GAAATGGGAG GTCACAGCCA 
ACTTCAGGGT GGAAGGGTGG TCCCCAGCCA ACTGTTTCCA AGGAGGAAGA GCCAAGTGGC 
TGGGAGGAAC CATCCTCACA ATCCATTGGT CATAAAATGG ACATTGATGA CGGAACAGCA 
GCTTGGGGGG ATCCTAACAA TTACAATTAC AAAAATGTCA GCCTGTGGGA CAAGAATGCT 
CAAGCAGGCC CTGGGCAGAT GGCTCGGGAG CAAGGAATAT CTGGGACATT GGCAAACAAG 
GCAGCTCCCT CAGTGTGGAG TAAAAACAAA GGACCCCCAA CTGACAATGG GACATCAGCT 
TGGGGTGAGC CAACTGAACC TGCTTCAGGG TGGGGAGAAG GTGAGGAGTC GGGTCCACCA 
ACACCAGGAT GGGGAAGTGC ACCCCAAGTA AATCCCAGTG CCATGAAATC TGGTACAAAA 
TCTATGCAAG ATGGCTGGGG TGAGAGTGAT GGGCCGGTGA CAGGAACACG TCACAGCAGC 
TGGGATGAAG AAGAAGAGGG TGGAGTCTGG AACAGTGCAG GTTCTCAGGG AAGTGGCTCC 
TCCCACAACT CTGCAGGTTG GGGACAAGGA GGAAAGAAAC CACAGATCAA GGGTCCCTTA 
AAAGGAGGGA GCAATGATAC CTGGATGAAC ACCTTGCCCA AACAGTTCTC CAACATGGGA 
TTGTTGAGCC AAGTTGATGA CCCCTCTACC ACTAAGATGG ACCATCCTGG CGGGCTTCCA 
GATAAGAAGT TTGAGACAGA CAAGCGGGGC GTGAATCTCG GGGATTTTAA TGACATGATG 
CGGAAGGAAC GGTCTGGCTT CCGTCTGAAC AATTCCAAAG AGATGGGAGC TGCAGACAGT 
GGGCCTTATT TTGAGAAGGG TGGCAATCAC GGACTATTTG GAAACAGCGG TGCACAATCG 
CGAGGCATGC ACAACCCTGT GCCATCGCTC AACTCCTCTC CCAACCTCCG GGCACAAGTG 
CCTCCCCAGT TCCTCTCTCC CCAGGTCTCA GCCTCCATGC TAAAGCAGTT TCCTAACAGT 
AACATGAATC CAGGACTTTT CAATATGGGG CCACAGCTTT CCCCTCAGCA GATTGCAATG 
CTGAGCCAGC TTCCTCAAAT TCAGCAGTTC CAGCTGGCAT GTCAGCTCCT CTTGCAACAA 
CAACAACAGC AGCAGCAACA GCAGCAGCAG CAGCAGCAAC TCCTTCAGAA CCAGAGAAAG 
ATCTCACAAG CGGTCAGACA GCAGCAGGAA CAGCAGATAG CACGCATGGT AAGTGCCCTA 
CAACAGCAGC AACAGCAACA GCAGCAGCAG CAACAGAGGC AGCCAGGAAT GAAGCACTCT 
CCCTCTCACC CTGCTGGACC CAAAGGTCAC TTAGATGCTG TTGGACTCTC AGACATGCAA 
GCCAAAGGGC ACATGCCATA TGGATCTGGT TTTGGCTCAA GTGGGATGGA TTATGGTACA 
GTAGGTAAGG AGGCGGGAGC AGAGTCTCGA TTTAAACAGT GGACATCCAT GATGGAGGGG 
CTACCGTCCA CTGTTTCTCA AGATACCAGC CTGCATAAAA ATGGCTCCAT TGCACCCCCA 
GTAAAAAATC GTGGAGGTTC CCCCTACCAC CAATATGACA TGTTGTCTAC GGATGCTTTG 
GGAGGGCATG GGGGGCCAAC AGCTGAGAGC TGGTTACCTG CCAAATCACC CCCTTCAAGC 
AAAATTGGAA ACAAGTCAAT CAATGCCAGC TGGCCCCCAG AGTTTCAGCC AGGAGTGCCA 
TGGAAAGGGA TCCAAAATAT TGATCCAGAA TCTGATCCTT ATGTTACCCC TGGCAGTGTG 
CTTGGGGGTC CAGCCCCATC TCCCATTGTA GATACTGACC ACCAGCTACT GCAGGACACC 
CCCACAGGGT CCAATTCTTC CCTCAACACC TCGCTGCCTT CACCTGGTGC CTGGCCCTTT 
AGTGCCTCTG AGAATATCCA TAGCACTTCA GCACTCTCTG CAAAGTTTGG GGACTACAAA 
TCGACCTGGT CACCAGACCC CATAGGACAT AACCCATCAC ATCACTCAAA CAAGATGTGG 
AAAAACCAGA TGTCCAGGAA CAGCCCTGGG CTGCCTCGCC CACCTCCAGG GCTCACAAAC 
AACCCCAAAG CCACCAACTC TCCCTGGAAC AGCACAGCAC CTCGCTCCGT CAGGGGCTGG 
GGCGGACAGG ACTCCAGAAT GGCTTCTGCA TCAGCTTGGA GTGATGGTGG ATCTGTGCGC 
CCCAGCTACT GGCTGATCCT TCTTAATCTC ACACCACAGA TCGATGGTTC CACCCTGCGA 
ACGATCTGCA TGCAGCATGG CCCCCTGCTG ACATTCCATC TCAACCTTAC CCAGGGCACA 
GCTCTAATCC GATACAGCAC CAAACAGGAG GCAGCCAAGG CCCAGAGTGC CCTCCATATG 
TGTGTTCTGG GTAATACAAC TATCCTGGCG GAGTTTGCAA CGGATGAGGA GGTGAGCCGG 
TATCTTGCAC AAGCTCAGCC ACCAGCTCCC TCTGCTCCAG GAGCAGGCTG GCAGCCTTTG 
GAAGTGGGAC CGAACCACCC AGCTGAACCA GTAGGGCCAG CCCTAAACCT TTTTGGAGGA 
ACCACAGGGC TAGGGCAATG GAGCAGCGGC GGTGGTGGTA GTGACCTGTC AGGGGTTTCT 
CTCTGGGGTA CTCCTAGCAG CTACACATCC AGTCTGTGGG GTGTGCCGAG CACAGAGGAC 
GCCCACAGGA TGGGCAGCCC TGCCCCGTTA CTACCCGGTG ACCTTCTGGG AGGAGGGACC 
GACTCGATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949145.1
CDS	49..5520
Translation	MQNGCQSLVYAHFPLKRRKQKYINKEKEQEREEQLMEDKKRKKEDKKKKEAAQKVTEQKTKVPEVTKPSSSQATAASLPGSSPSPPVNGGNNAKRVAVPNGQPPSTARYMPREVPPRFRCQQDHKVLLKRGQPPPASCMLPGGGAGTPPSTTAPGAGPGNAQPGAGTLLPGESGASTDSSSGAAATSNYANSTWGSGGPTSSGPTTNPPHAWDKVIVDGSDMEEWPCIAGVDAEPPSDNATDNNSASNSASEKGPPQGSTTSHKGRGGQGQQQQQQQPGSECIQGVWKSDPKAKPVPSSNPASEGVNRLGNWRNGGGSERAGSASGFSNFNPNTNPSAWPALVQEGNRKGNPESDSSFSGAQLGPVGLTSREQQSKMENAGSSFAGSGRESATTHHTDGPKNGNTNSTNSNTLNPPDNKGTAFGVGSMDSCRGSDSPAQSTGERKNGGIGSWGAGRGQSATGDANCGHSNSGSNGREREDARKAPSSHRPNGSRGESWESKSGNGSWGYSPQADLSEVKWSEGNKLGAGGASQGEWKQPAGHEDLKVGEWGGLNQGNSSTGAWDNQKGHSLSDNQGNTSSAACWGRAPSSAGSEAGGQSTGSNPKAGGSGGSGDSLNSSSRRSHRPALPDSQAALQTLLNRTDLDPRVLSNSGWGQTQIKQDASWESEEVPSRTEGKSDRGTEGWENVSQAKTSGGWGDAPSQSNQNKSGWGEPPVPAPSTQEWKDTKATGWNDYKNSPTGWVGGGGGGVVGARPEDKAVPGWNNESGGAAEPGWSTRQSNPGGGWSAGKNGWGGNGSEDMEPPKAPGGWEGSGSKSSSGWGEVGQNDVGSWGNGANSNPAPRTGWEETKRPQGGGNWGEAARPQLPWNKQQESTPSSATPSTWGGSQAPPPPGNGRSQPTSGWKGGPQPTVSKEEEPSGWEEPSSQSIGHKMDIDDGTAAWGDPNNYNYKNVSLWDKNAQAGPGQMAREQGISGTLANKAAPSVWSKNKGPPTDNGTSAWGEPTEPASGWGEGEESGPPTPGWGSAPQVNPSAMKSGTKSMQDGWGESDGPVTGTRHSSWDEEEEGGVWNSAGSQGSGSSHNSAGWGQGGKKPQIKGPLKGGSNDTWMNTLPKQFSNMGLLSQVDDPSTTKMDHPGGLPDKKFETDKRGVNLGDFNDMMRKERSGFRLNNSKEMGAADSGPYFEKGGNHGLFGNSGAQSRGMHNPVPSLNSSPNLRAQVPPQFLSPQVSASMLKQFPNSNMNPGLFNMGPQLSPQQIAMLSQLPQIQQFQLACQLLLQQQQQQQQQQQQQQQLLQNQRKISQAVRQQQEQQIARMVSALQQQQQQQQQQQQRQPGMKHSPSHPAGPKGHLDAVGLSDMQAKGHMPYGSGFGSSGMDYGTVGKEAGAESRFKQWTSMMEGLPSTVSQDTSLHKNGSIAPPVKNRGGSPYHQYDMLSTDALGGHGGPTAESWLPAKSPPSSKIGNKSINASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGPAPSPIVDTDHQLLQDTPTGSNSSLNTSLPSPGAWPFSASENIHSTSALSAKFGDYKSTWSPDPIGHNPSHHSNKMWKNQMSRNSPGLPRPPPGLTNNPKATNSPWNSTAPRSVRGWGGQDSRMASASAWSDGGSVRPSYWLILLNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQSALHMCVLGNTTILAEFATDEEVSRYLAQAQPPAPSAPGAGWQPLEVGPNHPAEPVGPALNLFGGTTGLGQWSSGGGGSDLSGVSLWGTPSSYTSSLWGVPSTEDAHRMGSPAPLLPGDLLGGGTDSI

Target ORF information:

RefSeq Version	XM_018093656.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trinucleotide repeat containing 6B (tnrc6b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093656.1

ORF Insert Sequence:

ATGCAAAATG GCTGCCAGTC TCTCGTTTAT GCACATTTCC CTTTAAAGAG GAGAAAACAG 
AAATATATAA ACAAAGAGAA GGAGCAAGAA AGGGAAGAAC AGTTAATGGA AGACAAGAAA 
AGGAAGAAAG AGGATAAAAA GAAGAAAGAA GCTGCTCAGA AGGTCACAGA ACAAAAAACC 
AAAGTGCCCG AAGTGACGAA ACCAAGTTCA AGCCAAGCGA CGGCCGCCAG CCTACCTGGC 
AGCTCCCCCT CGCCACCAGT CAATGGTGGC AACAATGCCA AAAGGGTGGC AGTGCCGAAC 
GGACAACCGC CCAGCACCGC CCGCTATATG CCTCGGGAGG TGCCGCCGCG ATTCCGCTGC 
CAGCAGGACC ACAAAGTGTT ACTGAAGCGT GGACAGCCTC CTCCAGCGTC CTGTATGCTG 
CCGGGGGGAG GAGCAGGGAC TCCCCCCTCA ACCACTGCTC CAGGGGCCGG CCCAGGCAAC 
GCACAGCCAG GGGCGGGGAC ACTGCTGCCA GGGGAAAGCG GGGCTTCAAC AGACTCATCA 
TCGGGGGCAG CTGCTACTTC AAATTATGCA AATTCCACTT GGGGCTCAGG AGGCCCCACC 
AGCAGCGGCC CCACCACAAA CCCTCCCCAC GCCTGGGACA AAGTGATTGT AGACGGGTCG 
GACATGGAAG AGTGGCCTTG CATAGCCGGC GTGGACGCAG AGCCCCCTTC CGACAACGCC 
ACAGACAACA ACAGTGCCTC GAACTCAGCC TCGGAGAAGG GACCCCCACA AGGAAGCACC 
ACTAGCCACA AAGGGCGAGG GGGCCAGGGC CAGCAGCAAC AGCAGCAGCA GCCTGGTAGT 
GAATGTATCC AGGGCGTCTG GAAATCTGAC CCCAAGGCTA AGCCCGTCCC GTCTTCCAAC 
CCTGCTTCAG AGGGTGTAAA TAGACTGGGA AACTGGAGAA ATGGGGGTGG GTCAGAGAGA 
GCAGGTTCTG CCTCTGGTTT CAGCAACTTT AATCCAAATA CTAACCCATC TGCTTGGCCT 
GCCCTTGTCC AGGAAGGCAA CAGGAAAGGG AACCCAGAGT CAGACAGCAG CTTTTCTGGC 
GCACAACTCG GCCCAGTGGG TTTGACCTCT CGGGAGCAGC AGTCAAAGAT GGAAAATGCA 
GGTTCCAGCT TTGCAGGATC GGGCAGGGAG TCTGCCACAA CCCATCACAC TGACGGACCA 
AAAAATGGAA ACACTAACTC TACGAACTCA AATACTTTGA ACCCTCCTGA TAACAAGGGA 
ACAGCTTTTG GGGTGGGCTC AATGGATTCC TGCCGGGGTT CAGACTCCCC TGCTCAAAGC 
ACTGGAGAAA GAAAGAATGG GGGGATTGGC TCTTGGGGAG CGGGAAGGGG TCAGTCTGCC 
ACAGGAGATG CTAATTGTGG ACATAGCAAC TCTGGGAGCA ATGGACGAGA GAGAGAAGAC 
GCAAGGAAAG CGCCTTCATC TCATAGACCC AATGGGTCAA GGGGGGAATC TTGGGAAAGC 
AAAAGTGGGA ATGGTTCTTG GGGTTACAGC CCGCAGGCAG ACCTCAGTGA AGTGAAATGG 
AGTGAAGGGA ACAAATTGGG GGCTGGAGGG GCGTCTCAGG GAGAATGGAA GCAGCCAGCC 
GGGCATGAGG ACTTAAAGGT GGGAGAGTGG GGAGGCTTGA ACCAAGGAAA CTCTAGCACT 
GGAGCATGGG ACAATCAGAA GGGCCACTCT CTATCGGATA ACCAGGGCAA CACGTCATCA 
GCAGCCTGCT GGGGCCGAGC CCCCAGCTCT GCAGGAAGTG AAGCTGGGGG GCAAAGCACT 
GGAAGCAACC CTAAAGCAGG GGGGAGCGGA GGAAGCGGAG ACAGCCTGAA CTCCAGCAGT 
CGACGTTCAC ACAGACCTGC CCTCCCAGAC AGTCAGGCTG CTCTTCAGAC TTTACTGAAT 
CGCACTGATT TGGATCCCCG GGTGCTGTCC AACTCTGGCT GGGGCCAAAC CCAGATCAAG 
CAAGATGCAT CATGGGAGAG TGAGGAAGTG CCCTCTCGAA CTGAAGGCAA ATCAGACCGT 
GGGACTGAAG GTTGGGAGAA CGTGTCCCAA GCCAAAACCT CAGGGGGTTG GGGGGATGCT 
CCCAGCCAAA GCAATCAAAA CAAGTCAGGC TGGGGGGAGC CCCCTGTCCC TGCACCTTCA 
ACCCAAGAAT GGAAGGACAC CAAAGCAACT GGCTGGAATG ACTATAAAAA CAGCCCAACT 
GGTTGGGTTG GTGGAGGAGG TGGTGGGGTG GTTGGAGCAC GACCAGAGGA CAAGGCAGTT 
CCAGGTTGGA ACAATGAAAG TGGTGGGGCT GCGGAGCCTG GGTGGAGTAC TCGCCAGTCT 
AATCCAGGAG GTGGCTGGTC AGCAGGAAAG AATGGTTGGG GGGGAAATGG TAGTGAGGAC 
ATGGAGCCTC CCAAGGCCCC TGGAGGCTGG GAAGGCTCAG GAAGCAAATC CTCCTCTGGC 
TGGGGTGAAG TTGGACAAAA TGATGTGGGT TCTTGGGGTA ATGGGGCAAA CTCAAATCCA 
GCTCCACGAA CTGGCTGGGA GGAGACTAAG AGGCCCCAGG GAGGAGGAAA CTGGGGTGAG 
GCAGCTAGGC CCCAACTACC CTGGAACAAA CAGCAGGAAT CAACGCCATC TTCAGCAACT 
CCTAGCACCT GGGGGGGCTC TCAGGCTCCC CCGCCACCTG GAAATGGGAG GTCACAGCCA 
ACTTCAGGGT GGAAGGGTGG TCCCCAGCCA ACTGTTTCCA AGGAGGAAGA GCCAAGTGGC 
TGGGAGGAAC CATCCTCACA ATCCATTGGT CATAAAATGG ACATTGATGA CGGAACAGCA 
GCTTGGGGGG ATCCTAACAA TTACAATTAC AAAAATGTCA GCCTGTGGGA CAAGAATGCT 
CAAGCAGGCC CTGGGCAGAT GGCTCGGGAG CAAGGAATAT CTGGGACATT GGCAAACAAG 
GCAGCTCCCT CAGTGTGGAG TAAAAACAAA GGACCCCCAA CTGACAATGG GACATCAGCT 
TGGGGTGAGC CAACTGAACC TGCTTCAGGG TGGGGAGAAG GTGAGGAGTC GGGTCCACCA 
ACACCAGGAT GGGGAAGTGC ACCCCAAGTA AATCCCAGTG CCATGAAATC TGGTACAAAA 
TCTATGCAAG ATGGCTGGGG TGAGAGTGAT GGGCCGGTGA CAGGAACACG TCACAGCAGC 
TGGGATGAAG AAGAAGAGGG TGGAGTCTGG AACAGTGCAG GTTCTCAGGG AAGTGGCTCC 
TCCCACAACT CTGCAGGTTG GGGACAAGGA GGAAAGAAAC CACAGATCAA GGGTCCCTTA 
AAAGGAGGGA GCAATGATAC CTGGATGAAC ACCTTGCCCA AACAGTTCTC CAACATGGGA 
TTGTTGAGCC AAGTTGATGA CCCCTCTACC ACTAAGATGG ACCATCCTGG CGGGCTTCCA 
GATAAGAAGT TTGAGACAGA CAAGCGGGGC GTGAATCTCG GGGATTTTAA TGACATGATG 
CGGAAGGAAC GGTCTGGCTT CCGTCTGAAC AATTCCAAAG AGATGGGAGC TGCAGACAGT 
GGGCCTTATT TTGAGAAGGG TGGCAATCAC GGACTATTTG GAAACAGCGG TGCACAATCG 
CGAGGCATGC ACAACCCTGT GCCATCGCTC AACTCCTCTC CCAACCTCCG GGCACAAGTG 
CCTCCCCAGT TCCTCTCTCC CCAGGTCTCA GCCTCCATGC TAAAGCAGTT TCCTAACAGT 
AACATGAATC CAGGACTTTT CAATATGGGG CCACAGCTTT CCCCTCAGCA GATTGCAATG 
CTGAGCCAGC TTCCTCAAAT TCAGCAGTTC CAGCTGGCAT GTCAGCTCCT CTTGCAACAA 
CAACAACAGC AGCAGCAACA GCAGCAGCAG CAGCAGCAAC TCCTTCAGAA CCAGAGAAAG 
ATCTCACAAG CGGTCAGACA GCAGCAGGAA CAGCAGATAG CACGCATGGT AAGTGCCCTA 
CAACAGCAGC AACAGCAACA GCAGCAGCAG CAACAGAGGC AGCCAGGAAT GAAGCACTCT 
CCCTCTCACC CTGCTGGACC CAAAGGTCAC TTAGATGCTG TTGGACTCTC AGACATGCAA 
GCCAAAGGGC ACATGCCATA TGGATCTGGT TTTGGCTCAA GTGGGATGGA TTATGGTACA 
GTAGGTAAGG AGGCGGGAGC AGAGTCTCGA TTTAAACAGT GGACATCCAT GATGGAGGGG 
CTACCGTCCA CTGTTTCTCA AGATACCAGC CTGCATAAAA ATGGCTCCAT TGCACCCCCA 
GTAAAAAATC GTGGAGGTTC CCCCTACCAC CAATATGACA TGTTGTCTAC GGATGCTTTG 
GGAGGGCATG GGGGGCCAAC AGCTGAGAGC TGGTTACCTG CCAAATCACC CCCTTCAAGC 
AAAATTGGAA ACAAGTCAAT CAATGCCAGC TGGCCCCCAG AGTTTCAGCC AGGAGTGCCA 
TGGAAAGGGA TCCAAAATAT TGATCCAGAA TCTGATCCTT ATGTTACCCC TGGCAGTGTG 
CTTGGGGGTC CAGCCCCATC TCCCATTGTA GATACTGACC ACCAGCTACT GCAGGACACC 
CCCACAGGGT CCAATTCTTC CCTCAACACC TCGCTGCCTT CACCTGGTGC CTGGCCCTTT 
AGTGCCTCTG AGAATATCCA TAGCACTTCA GCACTCTCTG CAAAGTTTGG GGACTACAAA 
TCGACCTGGT CACCAGACCC CATAGGACAT AACCCATCAC ATCACTCAAA CAAGATGTGG 
AAAAACCAGA TGTCCAGGAA CAGCCCTGGG CTGCCTCGCC CACCTCCAGG GCTCACAAAC 
AACCCCAAAG CCACCAACTC TCCCTGGAAC AGCACAGCAC CTCGCTCCGT CAGGGGCTGG 
GGCGGACAGG ACTCCAGAAT GGCTTCTGCA TCAGCTTGGA GTGATGGTGG ATCTGTGCGC 
CCCAGCTACT GGCTGATCCT TCTTAATCTC ACACCACAGA TCGATGGTTC CACCCTGCGA 
ACGATCTGCA TGCAGCATGG CCCCCTGCTG ACATTCCATC TCAACCTTAC CCAGGGCACA 
GCTCTAATCC GATACAGCAC CAAACAGGAG GCAGCCAAGG CCCAGAGTGC CCTCCATATG 
TGTGTTCTGG GTAATACAAC TATCCTGGCG GAGTTTGCAA CGGATGAGGA GGTGAGCCGG 
TATCTTGCAC AAGCTCAGCC ACCAGCTCCC TCTGCTCCAG GAGCAGGCTG GCAGCCTTTG 
GAAGTGGGAC CGAACCACCC AGCTGAACCA GTAGGGCCAG CCCTAAACCT TTTTGGAGGA 
ACCACAGGGC TAGGGCAATG GAGCAGCGGC GGTGGTGGTA GTGACCTGTC AGGGGTTTCT 
CTCTGGGGTA CTCCTAGCAG CTACACATCC AGTCTGTGGG GTGTGCCGAG CACAGAGGAC 
GCCCACAGGA TGGGCAGCCC TGCCCCGTTA CTACCCGGTG ACCTTCTGGG AGGAGGGACC 
GACTCGATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	tnrc6b
Entrez Gene ID	100498152
Full Name	trinucleotide repeat containing 6B
General protein information	Preferred Names trinucleotide repeat containing 6B Names trinucleotide repeat-containing gene 6B protein
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

	TNRC6B	XP_001101111.2
Canis lupus familiaris (dog)	TNRC6B	XP_005625909.1
Bos taurus (cattle)	TNRC6B	NP_001179584.1
Gallus gallus (chicken)	TNRC6B	XP_004937779.1
Danio rerio (zebrafish)	tnrc6b	XP_002663990.3
Xenopus tropicalis (tropical clawed frog)	tnrc6b	XP_002934255.2
Homo sapiens (human)	TNRC6B	NP_001155973.1
Pan troglodytes (chimpanzee)	TNRC6B	XP_003317299.1
Mus musculus (house mouse)	Tnrc6b	NP_659061.2
Rattus norvegicus (Norway rat)	Tnrc6b	NP_620200.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

tnrc6b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

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Target ORF information:

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ORF Insert Sequence:

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