map3k5 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012963761.2	XP_012819215.1	mitogen-activated protein kinase kinase kinase 5 isoform X4
XM_012963758.3	XP_012819212.1	mitogen-activated protein kinase kinase kinase 5 isoform X1
XM_012963758.2	XP_012819212.1	mitogen-activated protein kinase kinase kinase 5 isoform X1
XM_018094280.1	XP_017949769.1	mitogen-activated protein kinase kinase kinase 5 isoform X4
XM_012963762.2	XP_012819216.1	mitogen-activated protein kinase kinase kinase 5 isoform X5
XM_012963762.3	XP_012819216.1	mitogen-activated protein kinase kinase kinase 5 isoform X4
XM_012963759.2	XP_012819213.1	mitogen-activated protein kinase kinase kinase 5 isoform X2
XM_012963760.2	XP_012819214.1	mitogen-activated protein kinase kinase kinase 5 isoform X3
XM_012963759.3	XP_012819213.1	mitogen-activated protein kinase kinase kinase 5 isoform X2
XM_012963760.3	XP_012819214.1	mitogen-activated protein kinase kinase kinase 5 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa36964	XM_012963758.3 Latest version!	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa36965	XM_012963759.3 Latest version!	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa36966	XM_012963760.3 Latest version!	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa36968	XM_012963762.3 Latest version!	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa36967	XM_012963761.2 Latest version!	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa36967	XM_018094280.1 Latest version!	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OXa36964	XM_012963758.2	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa36968	XM_012963762.2	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa36965	XM_012963759.2	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa36966	XM_012963760.2	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
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map3k5 ( XM_012963758.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819212.1 Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa36964
Clone ID Related Accession (Same CDS sequence)	XM_012963758.3 , XM_012963758.2
Accession Version	XM_012963758.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963758.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTAA TGAGTGACAG CAGCGAAGGA ATCAGTTTCT CTGTACCATG CTCACGGCTG 
CCTGGCTCAA GCTGCTGTGC TCATGAAGAC ACAGGAGGGC TTACGCTACA GGAAAATGCC 
ACCATATGCG GTGGAACCAG GATCCCTGAA GCTGGAAGCT TCTGGCGTGA AGATTACCTC 
CGTGCTTCAG CGGTTGGAAG TGGTAAAGAT GCCCCATGCT ATGGAAAGTG GGTTAACCCC 
CCTCCTACAG CTAGCGGAGG TATTAGCCTT CGTAGGATTA CTGTGGTATA TGTTATCAAT 
GAAGTAAGTC CGGAGTTGCA TGCTTGTGAG GCTGCTGCAT TATACAGTTT GCAGGAGGCA 
TGTGAGAACC ATGGGGCACT GCTTGAGACC CTGGATTTCG GCAAACTGGA CTTTGGCGAG 
ACGTCAGTGC TAGACACCTT CTACAATGCA GATATTGCAG TGGTTGAGAT GAATGACGCT 
TTTCGACAGC CTTCTTTGTT CTACCATCTG GGAGTAAGAG AAAGCTTTAG CATGGCAAAC 
AACATAATTC TTTACTGTGA CACAAATTCA GACTCCCTGC AGTCACTGAA GGAAATTATC 
GGGCAAAAGA ATACTATGTG CACAGGTAAT TACACATTTA TTCCTTACAT GATCACGCCA 
CAAAACAAAG TGTTTTGCTG TGAGAGTATT TTCATGAGAG GAATGACTGA ACTAATGCCG 
TCAAACTTTG AGTTACTTCT TGGTCCGATC TGCCTGCCCC TAGTGGATCG CTTTGTTCAG 
ATTCTGAAGG TGGCACAGGC CACGTCATGC CAATATTTTC GAGAGGCAAT ACTAAATGAT 
ATTAGAAGGG CTCGGAACCA ATACACTGGG AAGGATCTGG CTGCTGAGCT AATAAGGATC 
CGCCAGCGCG TAGACAGCAT CGAGTTGTTA TCACCTGACA TCATTATCAA TCTGCTTTTG 
TCATACAGAG ATATCCAGGA CTACGATTCA GTTGTGACGT TGGTGGAAAT ATTGGAGAAA 
CTTCCAACCT TTAATTTATC TTCCAATCAC CATATAAAAT TTCATTATGC ATTTGCATTA 
AACAGGAGAA ATCATCCAGG TGACAGAGTA AAAGCCCTTG AAATAATGTT GCCTTTGGTG 
CAACTTGAAG ATCAAGTAGC TTCAGACATA TACTGCATTG TGGGAAGGAT CTACAAAGAT 
ATGTTCTTAG ATTCTAACTT CATGGATACC GAAAGTCGTG ATAATGCTAT ACATTGGTAC 
AGGAAGGCCT TTGAATCTGA ACCAACACTT CATTCTGGAA TCAATTATGC GGTGCTACTT 
CTAGCTGCTG GTCATCAATT TGAGACTTCT TTGGAACTCC GGAAAATCGG AGTTAAACTC 
AGTAGCCTGT TGGGTAAAAA AGGAAATCTC GAAAAACTCC AAAGTTATTG GGATGTTGGA 
TTTTATTTGG GGGCATGTAT ACTGGCCAAT GACTCATCAA AAGTTATCCA TGCAGCAGAA 
AAGCTTTTCA AGCTCAAAAC CCCAGCATGG TTCTTGAATT CAGTTGTGGA GACCATTTTG 
ATTTATCAGC ATTTTAAAAC GCCAAACCAA GAACAACATG TAACAAACCA TGAGCTGGTT 
GATTTTTGGA TGGATTTTCT TGTTGAGTCT ACAAGTAGCA ATGTGTCTGT ATTACGGTTT 
CCTGTCTTAA TACTAGAGCC AACAAAAATC TACCAGCCTT CCTACCTATC AATCAATAGT 
GAAGCTGAAG TTCAGACAAT ATCTATTTGG CACGTTCTAC CTGGCGATAA GAAAGGAATC 
CATGAATGGA ATTTCCCTGC AGATTCTATC AGAGGTGTAA GCATTTCAAA GTTTGAAGAA 
AGGTGCTGTT TTCTGTATGT TCTGAACAAC TCTGACGATT TTCAAATATA TTTCTGCACA 
GAGCTCCACT GCAAAAAATT CTTTGATAAA GTAAACCGTA TCAAAGAAGA GAATGGAAAG 
AACACAGAAG AAGGGGAGTG TGATGGAGAT TTAATGGAGT TCGACTATGA GTATGATGAA 
AATGGGGACA GAGTGGTTCT CGGTAAAGGG AAACATGGAG TGGTATATGC AGGAAGAGAC 
TTGAGTAACC AGGTCAGAAT TGCAATTAAA GAAGTTCCAG AGAGAGATGG TCGATGCTCT 
CAACCACTTC ATGAAGAGAT TGCTTTGCAT AAACGTCTAA AGCATAAAAA CATTGTTCAG 
TATTTGGGAT CATTCAGTAA AGGTGGTTTC ATTAAAATAT TTATGGAACA AGTACCTGGA 
GGAAGCCTCT CCGCACTTCT AAGGTCAAAA TGGGGACCCT TGAAAGACAA TGAGCAGACA 
ATCGGATTTT ATACCAAACA GATCCTTGAA GGTTTGAAAT ATCTCCATGA TAACCAGATA 
GTTCATAGAG ATATCAAGGG TGATAATGTT CTTATAAACA CATACAGTGG TGTTCTGAAA 
ATCTCTGATT TTGGCACTTC AAAGAGACTT GCTGGAATCA ACCCAAATAC AGAAACCTTC 
ACTGGCACTC TACAATATAT GGCACCAGAA ATTATAGACA AAGGACCAAG AGGATATGGA 
AAGCCAGCAG ACATTTGGTC TCTGGGATGC ACAGTTATAG AAATGGCAAC AGGAAAACCA 
CCATTTTATG AGCTTGGAGA ACCACAAGCT GCCATGTTTA AAGTGGGCAT GTTTAAAATT 
CATCCAGATA TCCCAGAATC TATGTCGTCT GAAGCTAAGG CATTTTTGTT GCGGTGCTTT 
GAACCAGATC CTGTGAAACG CGCTACTGCT ACTGAACTTA TAACGGATGA ATTCTTGAAG 
GTGTCCTCTA GGAAACGCAA GTCCCAGCTT AAGCTTACAA CTCTTTCCTC TGGCTCAAAT 
GAATATCTCC GTAGCATATC TTTGCCGGTG TCAGTGATGG TGGAAGATAC AAGCAGCAGC 
AGTGAATACA GTTCCGTTTC TCCAGATTCA GACTTAAGAT CGGACCCATT TACTTTTAAA 
ACAAGAGCCA GATCATGTAT AGAAAAGGAT GTCAAGCTTA ACAGAAGTTT ATTTCTAGGA 
ATTCCAGACG AGAATTTTGA AGACAGTAGT GCTCCTCCTT CTCCTGAAGA ACGAGACTCT 
GGATTTTTCA TGCTGAAGAA AGATAGTGAA AGGAGGGCTA CACTTTATCA TATTCTTACA 
GAGGATCAGG ACAAGGTGGC TAGAAACTTA ATGGATGCTT TAGTCCAGGG ATCAGAAATA 
AAATTGAAAT GGGACCACAT CACTGCTATT GTTGACAGCC TAAAAGAGTT TGTAAGATCT 
ACTGACAGGA AAATCATTTC AACAACACTA TCAAAACTCA AACTTGAGTT AGACTTTGAT 
AGTCATGCTA TCAGTCAAGT CCAGATGGTG CTGTTTGGCT TTCAAGATGC GGTAAATAAA 
GTGCTACGAA ATCATAACAT AAAGCCTCAC TGGATGTTTG CCTTAGATAA TATAATTCGA 
AAGGCCGTGC AGACAGCTAT CACTATCCTG GTACCTGAGC TGAGGCCACA TTTTAGCATT 
ACATCAGAAA GTGACCAAGC AGATCAGGAG GACATAGAAG TAGAGGAAAG AAGTAATAAA 
CAACCCACAG AACAAACCAT TCACAGAGAA GTCAATGAGG ACAACGTGCA AACCTCAGGA 
GTGAGCACAC TCAGTTCAAC TTTGTCCCAC AACTCTCAGA ATGCCGACAG GTCCCTGAAT 
GTGCAACTTG AAAGACTTAG GTTAGAGTCA AACAGGCTTT TGGAAGAATT GTTACTTAAA 
GAGAGAGAAT TCCAAGTCCT TCTACGCCAA GCTATTGAAG TGAAGGACAG GGAAATCAAA 
CGCCTGCAGT GCACGTCTGA GCCAATAAGC ACTTCCGTAT CCAGCACAAG GTCAACTATC 
TCAGGCAGTG AAGACTTGAA TCTTGTTTAC TGGCTACACA AGTGCAGAGT AGATGAAGAT 
ACAATAAGCA AGTTTTTGGC AGAAGACTAT CAGCTTGAAG ATATTCTTTG TCATGTCACA 
CGAGATGATT TAAAATCTCT GAATCTGAGG GGAGGAGTAC TATGCACAAT ATGGAAAGAA 
ATCACTGACT ACAGAGAAAA ACAAGCCTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819212.1
CDS	185..4297
Translation	MQVMSDSSEGISFSVPCSRLPGSSCCAHEDTGGLTLQENATICGGTRIPEAGSFWREDYLRASAVGSGKDAPCYGKWVNPPPTASGGISLRRITVVYVINEVSPELHACEAAALYSLQEACENHGALLETLDFGKLDFGETSVLDTFYNADIAVVEMNDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIIGQKNTMCTGNYTFIPYMITPQNKVFCCESIFMRGMTELMPSNFELLLGPICLPLVDRFVQILKVAQATSCQYFREAILNDIRRARNQYTGKDLAAELIRIRQRVDSIELLSPDIIINLLLSYRDIQDYDSVVTLVEILEKLPTFNLSSNHHIKFHYAFALNRRNHPGDRVKALEIMLPLVQLEDQVASDIYCIVGRIYKDMFLDSNFMDTESRDNAIHWYRKAFESEPTLHSGINYAVLLLAAGHQFETSLELRKIGVKLSSLLGKKGNLEKLQSYWDVGFYLGACILANDSSKVIHAAEKLFKLKTPAWFLNSVVETILIYQHFKTPNQEQHVTNHELVDFWMDFLVESTSSNVSVLRFPVLILEPTKIYQPSYLSINSEAEVQTISIWHVLPGDKKGIHEWNFPADSIRGVSISKFEERCCFLYVLNNSDDFQIYFCTELHCKKFFDKVNRIKEENGKNTEEGECDGDLMEFDYEYDENGDRVVLGKGKHGVVYAGRDLSNQVRIAIKEVPERDGRCSQPLHEEIALHKRLKHKNIVQYLGSFSKGGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPNTETFTGTLQYMAPEIIDKGPRGYGKPADIWSLGCTVIEMATGKPPFYELGEPQAAMFKVGMFKIHPDIPESMSSEAKAFLLRCFEPDPVKRATATELITDEFLKVSSRKRKSQLKLTTLSSGSNEYLRSISLPVSVMVEDTSSSSEYSSVSPDSDLRSDPFTFKTRARSCIEKDVKLNRSLFLGIPDENFEDSSAPPSPEERDSGFFMLKKDSERRATLYHILTEDQDKVARNLMDALVQGSEIKLKWDHITAIVDSLKEFVRSTDRKIISTTLSKLKLELDFDSHAISQVQMVLFGFQDAVNKVLRNHNIKPHWMFALDNIIRKAVQTAITILVPELRPHFSITSESDQADQEDIEVEERSNKQPTEQTIHREVNEDNVQTSGVSTLSSTLSHNSQNADRSLNVQLERLRLESNRLLEELLLKEREFQVLLRQAIEVKDREIKRLQCTSEPISTSVSSTRSTISGSEDLNLVYWLHKCRVDEDTISKFLAEDYQLEDILCHVTRDDLKSLNLRGGVLCTIWKEITDYREKQAS

Target ORF information:

RefSeq Version	XM_012963758.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963758.3

ORF Insert Sequence:

ATGCAAGTAA TGAGTGACAG CAGCGAAGGA ATCAGTTTCT CTGTACCATG CTCACGGCTG 
CCTGGCTCAA GCTGCTGTGC TCATGAAGAC ACAGGAGGGC TTACGCTACA GGAAAATGCC 
ACCATATGCG GTGGAACCAG GATCCCTGAA GCTGGAAGCT TCTGGCGTGA AGATTACCTC 
CGTGCTTCAG CGGTTGGAAG TGGTAAAGAT GCCCCATGCT ATGGAAAGTG GGTTAACCCC 
CCTCCTACAG CTAGCGGAGG TATTAGCCTT CGTAGGATTA CTGTGGTATA TGTTATCAAT 
GAAGTAAGTC CGGAGTTGCA TGCTTGTGAG GCTGCTGCAT TATACAGTTT GCAGGAGGCA 
TGTGAGAACC ATGGGGCACT GCTTGAGACC CTGGATTTCG GCAAACTGGA CTTTGGCGAG 
ACGTCAGTGC TAGACACCTT CTACAATGCA GATATTGCAG TGGTTGAGAT GAATGACGCT 
TTTCGACAGC CTTCTTTGTT CTACCATCTG GGAGTAAGAG AAAGCTTTAG CATGGCAAAC 
AACATAATTC TTTACTGTGA CACAAATTCA GACTCCCTGC AGTCACTGAA GGAAATTATC 
GGGCAAAAGA ATACTATGTG CACAGGTAAT TACACATTTA TTCCTTACAT GATCACGCCA 
CAAAACAAAG TGTTTTGCTG TGAGAGTATT TTCATGAGAG GAATGACTGA ACTAATGCCG 
TCAAACTTTG AGTTACTTCT TGGTCCGATC TGCCTGCCCC TAGTGGATCG CTTTGTTCAG 
ATTCTGAAGG TGGCACAGGC CACGTCATGC CAATATTTTC GAGAGGCAAT ACTAAATGAT 
ATTAGAAGGG CTCGGAACCA ATACACTGGG AAGGATCTGG CTGCTGAGCT AATAAGGATC 
CGCCAGCGCG TAGACAGCAT CGAGTTGTTA TCACCTGACA TCATTATCAA TCTGCTTTTG 
TCATACAGAG ATATCCAGGA CTACGATTCA GTTGTGACGT TGGTGGAAAT ATTGGAGAAA 
CTTCCAACCT TTAATTTATC TTCCAATCAC CATATAAAAT TTCATTATGC ATTTGCATTA 
AACAGGAGAA ATCATCCAGG TGACAGAGTA AAAGCCCTTG AAATAATGTT GCCTTTGGTG 
CAACTTGAAG ATCAAGTAGC TTCAGACATA TACTGCATTG TGGGAAGGAT CTACAAAGAT 
ATGTTCTTAG ATTCTAACTT CATGGATACC GAAAGTCGTG ATAATGCTAT ACATTGGTAC 
AGGAAGGCCT TTGAATCTGA ACCAACACTT CATTCTGGAA TCAATTATGC GGTGCTACTT 
CTAGCTGCTG GTCATCAATT TGAGACTTCT TTGGAACTCC GGAAAATCGG AGTTAAACTC 
AGTAGCCTGT TGGGTAAAAA AGGAAATCTC GAAAAACTCC AAAGTTATTG GGATGTTGGA 
TTTTATTTGG GGGCATGTAT ACTGGCCAAT GACTCATCAA AAGTTATCCA TGCAGCAGAA 
AAGCTTTTCA AGCTCAAAAC CCCAGCATGG TTCTTGAATT CAGTTGTGGA GACCATTTTG 
ATTTATCAGC ATTTTAAAAC GCCAAACCAA GAACAACATG TAACAAACCA TGAGCTGGTT 
GATTTTTGGA TGGATTTTCT TGTTGAGTCT ACAAGTAGCA ATGTGTCTGT ATTACGGTTT 
CCTGTCTTAA TACTAGAGCC AACAAAAATC TACCAGCCTT CCTACCTATC AATCAATAGT 
GAAGCTGAAG TTCAGACAAT ATCTATTTGG CACGTTCTAC CTGGCGATAA GAAAGGAATC 
CATGAATGGA ATTTCCCTGC AGATTCTATC AGAGGTGTAA GCATTTCAAA GTTTGAAGAA 
AGGTGCTGTT TTCTGTATGT TCTGAACAAC TCTGACGATT TTCAAATATA TTTCTGCACA 
GAGCTCCACT GCAAAAAATT CTTTGATAAA GTAAACCGTA TCAAAGAAGA GAATGGAAAG 
AACACAGAAG AAGGGGAGTG TGATGGAGAT TTAATGGAGT TCGACTATGA GTATGATGAA 
AATGGGGACA GAGTGGTTCT CGGTAAAGGG AAACATGGAG TGGTATATGC AGGAAGAGAC 
TTGAGTAACC AGGTCAGAAT TGCAATTAAA GAAGTTCCAG AGAGAGATGG TCGATGCTCT 
CAACCACTTC ATGAAGAGAT TGCTTTGCAT AAACGTCTAA AGCATAAAAA CATTGTTCAG 
TATTTGGGAT CATTCAGTAA AGGTGGTTTC ATTAAAATAT TTATGGAACA AGTACCTGGA 
GGAAGCCTCT CCGCACTTCT AAGGTCAAAA TGGGGACCCT TGAAAGACAA TGAGCAGACA 
ATCGGATTTT ATACCAAACA GATCCTTGAA GGTTTGAAAT ATCTCCATGA TAACCAGATA 
GTTCATAGAG ATATCAAGGG TGATAATGTT CTTATAAACA CATACAGTGG TGTTCTGAAA 
ATCTCTGATT TTGGCACTTC AAAGAGACTT GCTGGAATCA ACCCAAATAC AGAAACCTTC 
ACTGGCACTC TACAATATAT GGCACCAGAA ATTATAGACA AAGGACCAAG AGGATATGGA 
AAGCCAGCAG ACATTTGGTC TCTGGGATGC ACAGTTATAG AAATGGCAAC AGGAAAACCA 
CCATTTTATG AGCTTGGAGA ACCACAAGCT GCCATGTTTA AAGTGGGCAT GTTTAAAATT 
CATCCAGATA TCCCAGAATC TATGTCGTCT GAAGCTAAGG CATTTTTGTT GCGGTGCTTT 
GAACCAGATC CTGTGAAACG CGCTACTGCT ACTGAACTTA TAACGGATGA ATTCTTGAAG 
GTGTCCTCTA GGAAACGCAA GTCCCAGCTT AAGCTTACAA CTCTTTCCTC TGGCTCAAAT 
GAATATCTCC GTAGCATATC TTTGCCGGTG TCAGTGATGG TGGAAGATAC AAGCAGCAGC 
AGTGAATACA GTTCCGTTTC TCCAGATTCA GACTTAAGAT CGGACCCATT TACTTTTAAA 
ACAAGAGCCA GATCATGTAT AGAAAAGGAT GTCAAGCTTA ACAGAAGTTT ATTTCTAGGA 
ATTCCAGACG AGAATTTTGA AGACAGTAGT GCTCCTCCTT CTCCTGAAGA ACGAGACTCT 
GGATTTTTCA TGCTGAAGAA AGATAGTGAA AGGAGGGCTA CACTTTATCA TATTCTTACA 
GAGGATCAGG ACAAGGTGGC TAGAAACTTA ATGGATGCTT TAGTCCAGGG ATCAGAAATA 
AAATTGAAAT GGGACCACAT CACTGCTATT GTTGACAGCC TAAAAGAGTT TGTAAGATCT 
ACTGACAGGA AAATCATTTC AACAACACTA TCAAAACTCA AACTTGAGTT AGACTTTGAT 
AGTCATGCTA TCAGTCAAGT CCAGATGGTG CTGTTTGGCT TTCAAGATGC GGTAAATAAA 
GTGCTACGAA ATCATAACAT AAAGCCTCAC TGGATGTTTG CCTTAGATAA TATAATTCGA 
AAGGCCGTGC AGACAGCTAT CACTATCCTG GTACCTGAGC TGAGGCCACA TTTTAGCATT 
ACATCAGAAA GTGACCAAGC AGATCAGGAG GACATAGAAG TAGAGGAAAG AAGTAATAAA 
CAACCCACAG AACAAACCAT TCACAGAGAA GTCAATGAGG ACAACGTGCA AACCTCAGGA 
GTGAGCACAC TCAGTTCAAC TTTGTCCCAC AACTCTCAGA ATGCCGACAG GTCCCTGAAT 
GTGCAACTTG AAAGACTTAG GTTAGAGTCA AACAGGCTTT TGGAAGAATT GTTACTTAAA 
GAGAGAGAAT TCCAAGTCCT TCTACGCCAA GCTATTGAAG TGAAGGACAG GGAAATCAAA 
CGCCTGCAGT GCACGTCTGA GCCAATAAGC ACTTCCGTAT CCAGCACAAG GTCAACTATC 
TCAGGCAGTG AAGACTTGAA TCTTGTTTAC TGGCTACACA AGTGCAGAGT AGATGAAGAT 
ACAATAAGCA AGTTTTTGGC AGAAGACTAT CAGCTTGAAG ATATTCTTTG TCATGTCACA 
CGAGATGATT TAAAATCTCT GAATCTGAGG GGAGGAGTAC TATGCACAAT ATGGAAAGAA 
ATCACTGACT ACAGAGAAAA ACAAGCCTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k5 ( XM_012963759.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819213.1 Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa36965
Clone ID Related Accession (Same CDS sequence)	XM_012963759.2 , XM_012963759.3
Accession Version	XM_012963759.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963759.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTAA TGAGTGACAG CAGCGAAGGA ATCAGTTTCT CTGTACCATG CTCACGGCTG 
CCTGGCTCAA GCTGCTGTGC TCATGAAGAC ACAGGAGGGC TTACGCTACA GGAAAATGCC 
ACCATATGCG GTGGAACCAG GATCCCTGAA GCTGGAAGCT TCTGGCGTGA AGATTACCTC 
CGTGCTTCAG CGGTTGGAAG TGGTAAAGAT GCCCCATGCT ATGGAAAGTG GGTTAACCCC 
CCTCCTACAG CTAGCGGAGG TATTAGCCTT CGTAGGATTA CTGTGGTATA TGTTATCAAT 
GAAGTAAGTC CGGAGTTGCA TGCTTGTGAG GCTGCTGCAT TATACAGTTT GCAGGAGGCA 
TGTGAGAACC ATGGGGCACT GCTTGAGACC CTGGATTTCG GCAAACTGGA CTTTGGCGAG 
ACGTCAGTGC TAGACACCTT CTACAATGCA GATATTGCAG TGGTTGAGAT GAATGACGCT 
TTTCGACAGC CTTCTTTGTT CTACCATCTG GGAGTAAGAG AAAGCTTTAG CATGGCAAAC 
AACATAATTC TTTACTGTGA CACAAATTCA GACTCCCTGC AGTCACTGAA GGAAATTATC 
GGGCAAAAGA ATACTATGTG CACAGGTAAT TACACATTTA TTCCTTACAT GATCACGCCA 
CAAAACAAAG TGTTTTGCTG TGAGAGTATT TTCATGAGAG GAATGACTGA ACTAATGCCG 
TCAAACTTTG AGTTACTTCT TGGTCCGATC TGCCTGCCCC TAGTGGATCG CTTTGTTCAG 
ATTCTGAAGG TGGCACAGGC CACGTCATGC CAATATTTTC GAGAGGCAAT ACTAAATGAT 
ATTAGAAGGG CTCGGAACCA ATACACTGGG AAGGATCTGG CTGCTGAGCT AATAAGGATC 
CGCCAGCGCG TAGACAGCAT CGAGTTGTTA TCACCTGACA TCATTATCAA TCTGCTTTTG 
TCATACAGAG ATATCCAGGA CTACGATTCA GTTGTGACGT TGGTGGAAAT ATTGGAGAAA 
CTTCCAACCT TTAATTTATC TTCCAATCAC CATATAAAAT TTCATTATGC ATTTGCATTA 
AACAGGAGAA ATCATCCAGG TGACAGAGTA AAAGCCCTTG AAATAATGTT GCCTTTGGTG 
CAACTTGAAG ATCAAGTAGC TTCAGACATA TACTGCATTG TGGGAAGGAT CTACAAAGAT 
ATGTTCTTAG ATTCTAACTT CATGGATACC GAAAGTCGTG ATAATGCTAT ACATTGGTAC 
AGGAAGGCCT TTGAATCTGA ACCAACACTT CATTCTGGAA TCAATTATGC GGTGCTACTT 
CTAGCTGCTG GTCATCAATT TGAGACTTCT TTGGAACTCC GGAAAATCGG AGTTAAACTC 
AGTAGCCTGT TGGGTAAAAA AGGAAATCTC GAAAAACTCC AAAGTTATTG GGATGTTGGA 
TTTTATTTGG GGGCATGTAT ACTGGCCAAT GACTCATCAA AAGTTATCCA TGCAGCAGAA 
AAGCTTTTCA AGCTCAAAAC CCCAGCATGG TTCTTGAATT CAGTTGTGGA GACCATTTTG 
ATTTATCAGC ATTTTAAAAC GCCAAACCAA GAACAACATG TAACAAACCA TGAGCTGGTT 
GATTTTTGGA TGGATTTTCT TGTTGAGTCT ACAAGTAGCA ATGTGTCTGT ATTACGGTTT 
CCTGTCTTAA TACTAGAGCC AACAAAAATC TACCAGCCTT CCTACCTATC AATCAATAGT 
GAAGCTGAAG TTCAGACAAT ATCTATTTGG CACGTTCTAC CTGGCGATAA GAAAGGAATC 
CATGAATGGA ATTTCCCTGC AGATTCTATC AGAGGTGTAA GCATTTCAAA GTTTGAAGAA 
AGGTGCTGTT TTCTGTATGT TCTGAACAAC TCTGACGATT TTCAAATATA TTTCTGCACA 
GAGCTCCACT GCAAAAAATT CTTTGATAAA GTAAACCGTA TCAAAGAAGA GAATGGAAAG 
AACACAGAAG AAGGGGAGTG TGATGGAGAT TTAATGGAGT TCGACTATGA GTATGATGAA 
AATGGGGACA GAGTGGTTCT CGGTAAAGGG AAACATGGAG TGGTATATGC AGGAAGAGAC 
TTGAGTAACC AGGTCAGAAT TGCAATTAAA GAAGTTCCAG AGAGAGATGG TCGATGCTCT 
CAACCACTTC ATGAAGAGAT TGCTTTGCAT AAACGTCTAA AGCATAAAAA CATTGTTCAG 
TATTTGGGAT CATTCAGTAA AGGTGGTTTC ATTAAAATAT TTATGGAACA AGTACCTGGA 
GGAAGCCTCT CCGCACTTCT AAGGTCAAAA TGGGGACCCT TGAAAGACAA TGAGCAGACA 
ATCGGATTTT ATACCAAACA GATCCTTGAA GGTTTGAAAT ATCTCCATGA TAACCAGATA 
GTTCATAGAG ATATCAAGGG TGATAATGTT CTTATAAACA CATACAGTGG TGTTCTGAAA 
ATCTCTGATT TTGGCACTTC AAAGAGACTT GCTGGAATCA ACCCAAATAC AGAAACCTTC 
ACTGGCACTC TACAATATAT GGCACCAGAA ATTATAGACA AAGGACCAAG AGGATATGGA 
AAGCCAGCAG ACATTTGGTC TCTGGGATGC ACAGTTATAG AAATGGCAAC AGGAAAACCA 
CCATTTTATG AGCTTGGAGA ACCACAAGCT GCCATGTTTA AAGTGGGCAT GTTTAAAATT 
CATCCAGATA TCCCAGAATC TATGTCGTCT GAAGCTAAGG CATTTTTGTT GCGGTGCTTT 
GAACCAGATC CTGTGAAACG CGCTACTGCT ACTGAACTTA TAACGGATGA ATTCTTGAAG 
GTGTCCTCTA GGAAACGCAA GTCCCAGCTT AAGCTTACAA CTCTTTCCTC TGGCTCAAAT 
GAATATCTCC GTAGCATATC TTTGCCGGTG TCAGTGATGG TGGAAGATAC AAGCAGCAGC 
AGTGAATACA GTTCCGTTTC TCCAGATTCA GACTTAAGAT CGGACCCATT TACTTTTAAA 
ACAAGAGCCA GATCATGTAT AGAAAAGGAT GTCAAGCTTA ACAGAAGTTT ATTTCTAGGA 
ATTCCAGACG AGAATTTTGA AGACAGTAGT GCTCCTCCTT CTCCTGAAGA ACGAGACTCT 
GGATTTTTCA TGCTGAAGAA AGATAGTGAA AGGAGGGCTA CACTTTATCA TATTCTTACA 
GAGGATCAGG ACAAGGTGGC TAGAAACTTA ATGGATGCTT TAGTCCAGGG ATCAGAAATA 
AAATTGAAAT GGGACCACAT CACTGCTATT GTTGACAGCC TAAAAGAGTT TGTAAGATCT 
ACTGACAGGA AAATCATTTC AACAACACTA TCAAAACTCA AACTTGAGTT AGACTTTGAT 
AGTCATGCTA TCAGTCAAGT CCAGATGGTG CTGTTTGGCT TTCAAGATGC GGTAAATAAA 
GTGCTACGAA ATCATAACAT AAAGCCTCAC TGGATGTTTG CCTTAGATAA TATAATTCGA 
AAGGCCGTGC AGACAGCTAT CACTATCCTG GTACCTGAGC TGAGGCCACA TTTTAGCATT 
ACATCAGAAA GTGACCAAGC AGATCAGGAG GACATAGAAG TAGAGGAAAG AAGTAATAAA 
CAACCCACAG AACAAACCAT TCACAGAGAA GTCAATGAGG ACAACGTGCA AACCTCAGGA 
GTGAGCACAC TCAGTTCAAC TTTGTCCCAC AACTCTCAGA ATGCCGACAG GTCCCTGAAT 
GTGCAACTTG AAAGACTTAG GTTAGAGTCA AACAGGCTTT TGGAAGAATT GTTACTTAAA 
GAGAGAGAAT TCCAAGTCCT TCTACGCCAA GCTATTGAAG TGAAGGACAG GGAAATCAAA 
CGCCTGCAGT GCACGTCTGA GCCAATAAGC ACTTCCGTAT CCAGCACAAG GTCAACTATC 
TCAGGCAGTG AAGACTTGAA TCTTGTTTAC TGGCTACACA AGTGCAGAGT AGATGAAGAT 
ACAATAAGCA AGGGGAGGAG TACTATGCAC AATATGGAAA GAAATCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819213.1
CDS	185..4195
Translation	MQVMSDSSEGISFSVPCSRLPGSSCCAHEDTGGLTLQENATICGGTRIPEAGSFWREDYLRASAVGSGKDAPCYGKWVNPPPTASGGISLRRITVVYVINEVSPELHACEAAALYSLQEACENHGALLETLDFGKLDFGETSVLDTFYNADIAVVEMNDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIIGQKNTMCTGNYTFIPYMITPQNKVFCCESIFMRGMTELMPSNFELLLGPICLPLVDRFVQILKVAQATSCQYFREAILNDIRRARNQYTGKDLAAELIRIRQRVDSIELLSPDIIINLLLSYRDIQDYDSVVTLVEILEKLPTFNLSSNHHIKFHYAFALNRRNHPGDRVKALEIMLPLVQLEDQVASDIYCIVGRIYKDMFLDSNFMDTESRDNAIHWYRKAFESEPTLHSGINYAVLLLAAGHQFETSLELRKIGVKLSSLLGKKGNLEKLQSYWDVGFYLGACILANDSSKVIHAAEKLFKLKTPAWFLNSVVETILIYQHFKTPNQEQHVTNHELVDFWMDFLVESTSSNVSVLRFPVLILEPTKIYQPSYLSINSEAEVQTISIWHVLPGDKKGIHEWNFPADSIRGVSISKFEERCCFLYVLNNSDDFQIYFCTELHCKKFFDKVNRIKEENGKNTEEGECDGDLMEFDYEYDENGDRVVLGKGKHGVVYAGRDLSNQVRIAIKEVPERDGRCSQPLHEEIALHKRLKHKNIVQYLGSFSKGGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPNTETFTGTLQYMAPEIIDKGPRGYGKPADIWSLGCTVIEMATGKPPFYELGEPQAAMFKVGMFKIHPDIPESMSSEAKAFLLRCFEPDPVKRATATELITDEFLKVSSRKRKSQLKLTTLSSGSNEYLRSISLPVSVMVEDTSSSSEYSSVSPDSDLRSDPFTFKTRARSCIEKDVKLNRSLFLGIPDENFEDSSAPPSPEERDSGFFMLKKDSERRATLYHILTEDQDKVARNLMDALVQGSEIKLKWDHITAIVDSLKEFVRSTDRKIISTTLSKLKLELDFDSHAISQVQMVLFGFQDAVNKVLRNHNIKPHWMFALDNIIRKAVQTAITILVPELRPHFSITSESDQADQEDIEVEERSNKQPTEQTIHREVNEDNVQTSGVSTLSSTLSHNSQNADRSLNVQLERLRLESNRLLEELLLKEREFQVLLRQAIEVKDREIKRLQCTSEPISTSVSSTRSTISGSEDLNLVYWLHKCRVDEDTISKGRSTMHNMERNH

Target ORF information:

RefSeq Version	XM_012963759.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963759.3

ORF Insert Sequence:

ATGCAAGTAA TGAGTGACAG CAGCGAAGGA ATCAGTTTCT CTGTACCATG CTCACGGCTG 
CCTGGCTCAA GCTGCTGTGC TCATGAAGAC ACAGGAGGGC TTACGCTACA GGAAAATGCC 
ACCATATGCG GTGGAACCAG GATCCCTGAA GCTGGAAGCT TCTGGCGTGA AGATTACCTC 
CGTGCTTCAG CGGTTGGAAG TGGTAAAGAT GCCCCATGCT ATGGAAAGTG GGTTAACCCC 
CCTCCTACAG CTAGCGGAGG TATTAGCCTT CGTAGGATTA CTGTGGTATA TGTTATCAAT 
GAAGTAAGTC CGGAGTTGCA TGCTTGTGAG GCTGCTGCAT TATACAGTTT GCAGGAGGCA 
TGTGAGAACC ATGGGGCACT GCTTGAGACC CTGGATTTCG GCAAACTGGA CTTTGGCGAG 
ACGTCAGTGC TAGACACCTT CTACAATGCA GATATTGCAG TGGTTGAGAT GAATGACGCT 
TTTCGACAGC CTTCTTTGTT CTACCATCTG GGAGTAAGAG AAAGCTTTAG CATGGCAAAC 
AACATAATTC TTTACTGTGA CACAAATTCA GACTCCCTGC AGTCACTGAA GGAAATTATC 
GGGCAAAAGA ATACTATGTG CACAGGTAAT TACACATTTA TTCCTTACAT GATCACGCCA 
CAAAACAAAG TGTTTTGCTG TGAGAGTATT TTCATGAGAG GAATGACTGA ACTAATGCCG 
TCAAACTTTG AGTTACTTCT TGGTCCGATC TGCCTGCCCC TAGTGGATCG CTTTGTTCAG 
ATTCTGAAGG TGGCACAGGC CACGTCATGC CAATATTTTC GAGAGGCAAT ACTAAATGAT 
ATTAGAAGGG CTCGGAACCA ATACACTGGG AAGGATCTGG CTGCTGAGCT AATAAGGATC 
CGCCAGCGCG TAGACAGCAT CGAGTTGTTA TCACCTGACA TCATTATCAA TCTGCTTTTG 
TCATACAGAG ATATCCAGGA CTACGATTCA GTTGTGACGT TGGTGGAAAT ATTGGAGAAA 
CTTCCAACCT TTAATTTATC TTCCAATCAC CATATAAAAT TTCATTATGC ATTTGCATTA 
AACAGGAGAA ATCATCCAGG TGACAGAGTA AAAGCCCTTG AAATAATGTT GCCTTTGGTG 
CAACTTGAAG ATCAAGTAGC TTCAGACATA TACTGCATTG TGGGAAGGAT CTACAAAGAT 
ATGTTCTTAG ATTCTAACTT CATGGATACC GAAAGTCGTG ATAATGCTAT ACATTGGTAC 
AGGAAGGCCT TTGAATCTGA ACCAACACTT CATTCTGGAA TCAATTATGC GGTGCTACTT 
CTAGCTGCTG GTCATCAATT TGAGACTTCT TTGGAACTCC GGAAAATCGG AGTTAAACTC 
AGTAGCCTGT TGGGTAAAAA AGGAAATCTC GAAAAACTCC AAAGTTATTG GGATGTTGGA 
TTTTATTTGG GGGCATGTAT ACTGGCCAAT GACTCATCAA AAGTTATCCA TGCAGCAGAA 
AAGCTTTTCA AGCTCAAAAC CCCAGCATGG TTCTTGAATT CAGTTGTGGA GACCATTTTG 
ATTTATCAGC ATTTTAAAAC GCCAAACCAA GAACAACATG TAACAAACCA TGAGCTGGTT 
GATTTTTGGA TGGATTTTCT TGTTGAGTCT ACAAGTAGCA ATGTGTCTGT ATTACGGTTT 
CCTGTCTTAA TACTAGAGCC AACAAAAATC TACCAGCCTT CCTACCTATC AATCAATAGT 
GAAGCTGAAG TTCAGACAAT ATCTATTTGG CACGTTCTAC CTGGCGATAA GAAAGGAATC 
CATGAATGGA ATTTCCCTGC AGATTCTATC AGAGGTGTAA GCATTTCAAA GTTTGAAGAA 
AGGTGCTGTT TTCTGTATGT TCTGAACAAC TCTGACGATT TTCAAATATA TTTCTGCACA 
GAGCTCCACT GCAAAAAATT CTTTGATAAA GTAAACCGTA TCAAAGAAGA GAATGGAAAG 
AACACAGAAG AAGGGGAGTG TGATGGAGAT TTAATGGAGT TCGACTATGA GTATGATGAA 
AATGGGGACA GAGTGGTTCT CGGTAAAGGG AAACATGGAG TGGTATATGC AGGAAGAGAC 
TTGAGTAACC AGGTCAGAAT TGCAATTAAA GAAGTTCCAG AGAGAGATGG TCGATGCTCT 
CAACCACTTC ATGAAGAGAT TGCTTTGCAT AAACGTCTAA AGCATAAAAA CATTGTTCAG 
TATTTGGGAT CATTCAGTAA AGGTGGTTTC ATTAAAATAT TTATGGAACA AGTACCTGGA 
GGAAGCCTCT CCGCACTTCT AAGGTCAAAA TGGGGACCCT TGAAAGACAA TGAGCAGACA 
ATCGGATTTT ATACCAAACA GATCCTTGAA GGTTTGAAAT ATCTCCATGA TAACCAGATA 
GTTCATAGAG ATATCAAGGG TGATAATGTT CTTATAAACA CATACAGTGG TGTTCTGAAA 
ATCTCTGATT TTGGCACTTC AAAGAGACTT GCTGGAATCA ACCCAAATAC AGAAACCTTC 
ACTGGCACTC TACAATATAT GGCACCAGAA ATTATAGACA AAGGACCAAG AGGATATGGA 
AAGCCAGCAG ACATTTGGTC TCTGGGATGC ACAGTTATAG AAATGGCAAC AGGAAAACCA 
CCATTTTATG AGCTTGGAGA ACCACAAGCT GCCATGTTTA AAGTGGGCAT GTTTAAAATT 
CATCCAGATA TCCCAGAATC TATGTCGTCT GAAGCTAAGG CATTTTTGTT GCGGTGCTTT 
GAACCAGATC CTGTGAAACG CGCTACTGCT ACTGAACTTA TAACGGATGA ATTCTTGAAG 
GTGTCCTCTA GGAAACGCAA GTCCCAGCTT AAGCTTACAA CTCTTTCCTC TGGCTCAAAT 
GAATATCTCC GTAGCATATC TTTGCCGGTG TCAGTGATGG TGGAAGATAC AAGCAGCAGC 
AGTGAATACA GTTCCGTTTC TCCAGATTCA GACTTAAGAT CGGACCCATT TACTTTTAAA 
ACAAGAGCCA GATCATGTAT AGAAAAGGAT GTCAAGCTTA ACAGAAGTTT ATTTCTAGGA 
ATTCCAGACG AGAATTTTGA AGACAGTAGT GCTCCTCCTT CTCCTGAAGA ACGAGACTCT 
GGATTTTTCA TGCTGAAGAA AGATAGTGAA AGGAGGGCTA CACTTTATCA TATTCTTACA 
GAGGATCAGG ACAAGGTGGC TAGAAACTTA ATGGATGCTT TAGTCCAGGG ATCAGAAATA 
AAATTGAAAT GGGACCACAT CACTGCTATT GTTGACAGCC TAAAAGAGTT TGTAAGATCT 
ACTGACAGGA AAATCATTTC AACAACACTA TCAAAACTCA AACTTGAGTT AGACTTTGAT 
AGTCATGCTA TCAGTCAAGT CCAGATGGTG CTGTTTGGCT TTCAAGATGC GGTAAATAAA 
GTGCTACGAA ATCATAACAT AAAGCCTCAC TGGATGTTTG CCTTAGATAA TATAATTCGA 
AAGGCCGTGC AGACAGCTAT CACTATCCTG GTACCTGAGC TGAGGCCACA TTTTAGCATT 
ACATCAGAAA GTGACCAAGC AGATCAGGAG GACATAGAAG TAGAGGAAAG AAGTAATAAA 
CAACCCACAG AACAAACCAT TCACAGAGAA GTCAATGAGG ACAACGTGCA AACCTCAGGA 
GTGAGCACAC TCAGTTCAAC TTTGTCCCAC AACTCTCAGA ATGCCGACAG GTCCCTGAAT 
GTGCAACTTG AAAGACTTAG GTTAGAGTCA AACAGGCTTT TGGAAGAATT GTTACTTAAA 
GAGAGAGAAT TCCAAGTCCT TCTACGCCAA GCTATTGAAG TGAAGGACAG GGAAATCAAA 
CGCCTGCAGT GCACGTCTGA GCCAATAAGC ACTTCCGTAT CCAGCACAAG GTCAACTATC 
TCAGGCAGTG AAGACTTGAA TCTTGTTTAC TGGCTACACA AGTGCAGAGT AGATGAAGAT 
ACAATAAGCA AGGGGAGGAG TACTATGCAC AATATGGAAA GAAATCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k5 ( XM_012963760.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819214.1 Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X5, mRNA.

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CloneID	OXa36966
Clone ID Related Accession (Same CDS sequence)	XM_012963760.2 , XM_012963760.3
Accession Version	XM_012963760.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963760.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTAA TGAGTGACAG CAGCGAAGGA ATCAGTTTCT CTGTACCATG CTCACGGCTG 
CCTGGCTCAA GCTGCTGTGC TCATGAAGAC ACAGGAGGGC TTACGCTACA GGAAAATGCC 
ACCATATGCG GTGGAACCAG GATCCCTGAA GCTGGAAGCT TCTGGCGTGA AGATTACCTC 
CGTGCTTCAG CGGTTGGAAG TGGTAAAGAT GCCCCATGCT ATGGAAAGTG GGTTAACCCC 
CCTCCTACAG CTAGCGGAGG TATTAGCCTT CGTAGGATTA CTGTGGTATA TGTTATCAAT 
GAAGTAAGTC CGGAGTTGCA TGCTTGTGAG GCTGCTGCAT TATACAGTTT GCAGGAGGCA 
TGTGAGAACC ATGGGGCACT GCTTGAGACC CTGGATTTCG GCAAACTGGA CTTTGGCGAG 
ACGTCAGTGC TAGACACCTT CTACAATGCA GATATTGCAG TGGTTGAGAT GAATGACGCT 
TTTCGACAGC CTTCTTTGTT CTACCATCTG GGAGTAAGAG AAAGCTTTAG CATGGCAAAC 
AACATAATTC TTTACTGTGA CACAAATTCA GACTCCCTGC AGTCACTGAA GGAAATTATC 
GGGCAAAAGA ATACTATGTG CACAGGTAAT TACACATTTA TTCCTTACAT GATCACGCCA 
CAAAACAAAG TGTTTTGCTG TGAGAGTATT TTCATGAGAG GAATGACTGA ACTAATGCCG 
TCAAACTTTG AGTTACTTCT TGGTCCGATC TGCCTGCCCC TAGTGGATCG CTTTGTTCAG 
ATTCTGAAGG TGGCACAGGC CACGTCATGC CAATATTTTC GAGAGGCAAT ACTAAATGAT 
ATTAGAAGGG CTCGGAACCA ATACACTGGG AAGGATCTGG CTGCTGAGCT AATAAGGATC 
CGCCAGCGCG TAGACAGCAT CGAGTTGTTA TCACCTGACA TCATTATCAA TCTGCTTTTG 
TCATACAGAG ATATCCAGGA CTACGATTCA GTTGTGACGT TGGTGGAAAT ATTGGAGAAA 
CTTCCAACCT TTAATTTATC TTCCAATCAC CATATAAAAT TTCATTATGC ATTTGCATTA 
AACAGGAGAA ATCATCCAGG TGACAGAGTA AAAGCCCTTG AAATAATGTT GCCTTTGGTG 
CAACTTGAAG ATCAAGTAGC TTCAGACATA TACTGCATTG TGGGAAGGAT CTACAAAGAT 
ATGTTCTTAG ATTCTAACTT CATGGATACC GAAAGTCGTG ATAATGCTAT ACATTGGTAC 
AGGAAGGCCT TTGAATCTGA ACCAACACTT CATTCTGGAA TCAATTATGC GGTGCTACTT 
CTAGCTGCTG GTCATCAATT TGAGACTTCT TTGGAACTCC GGAAAATCGG AGTTAAACTC 
AGTAGCCTGT TGGGTAAAAA AGGAAATCTC GAAAAACTCC AAAGTTATTG GGATGTTGGA 
TTTTATTTGG GGGCATGTAT ACTGGCCAAT GACTCATCAA AAGTTATCCA TGCAGCAGAA 
AAGCTTTTCA AGCTCAAAAC CCCAGCATGG TTCTTGAATT CAGTTGTGGA GACCATTTTG 
ATTTATCAGC ATTTTAAAAC GCCAAACCAA GAACAACATG TAACAAACCA TGAGCTGGTT 
GATTTTTGGA TGGATTTTCT TGTTGAGTCT ACAAGTAGCA ATGTGTCTGT ATTACGGTTT 
CCTGTCTTAA TACTAGAGCC AACAAAAATC TACCAGCCTT CCTACCTATC AATCAATAGT 
GAAGCTGAAG TTCAGACAAT ATCTATTTGG CACGTTCTAC CTGGCGATAA GAAAGGAATC 
CATGAATGGA ATTTCCCTGC AGATTCTATC AGAGGTGTAA GCATTTCAAA GTTTGAAGAA 
AGGTGCTGTT TTCTGTATGT TCTGAACAAC TCTGACGATT TTCAAATATA TTTCTGCACA 
GAGCTCCACT GCAAAAAATT CTTTGATAAA GTAAACCGTA TCAAAGAAGA GAATGGAAAG 
AACACAGAAG AAGGGGAGTG TGATGGAGAT TTAATGGAGT TCGACTATGA GTATGATGAA 
AATGGGGACA GAGTGGTTCT CGGTAAAGGG AAACATGGAG TGGTATATGC AGGAAGAGAC 
TTGAGTAACC AGGTCAGAAT TGCAATTAAA GAAGTTCCAG AGAGAGATGG TCGATGCTCT 
CAACCACTTC ATGAAGAGAT TGCTTTGCAT AAACGTCTAA AGCATAAAAA CATTGTTCAG 
TATTTGGGAT CATTCAGTAA AGGTGGTTTC ATTAAAATAT TTATGGAACA AGTACCTGGA 
GGAAGCCTCT CCGCACTTCT AAGGTCAAAA TGGGGACCCT TGAAAGACAA TGAGCAGACA 
ATCGGATTTT ATACCAAACA GATCCTTGAA GGTTTGAAAT ATCTCCATGA TAACCAGATA 
GTTCATAGAG ATATCAAGGG TGATAATGTT CTTATAAACA CATACAGTGG TGTTCTGAAA 
ATCTCTGATT TTGGCACTTC AAAGAGACTT GCTGGAATCA ACCCAAATAC AGAAACCTTC 
ACTGGCACTC TACAATATAT GGCACCAGAA ATTATAGACA AAGGACCAAG AGGATATGGA 
AAGCCAGCAG ACATTTGGTC TCTGGGATGC ACAGTTATAG AAATGGCAAC AGGAAAACCA 
CCATTTTATG AGCTTGGAGA ACCACAAGCT GCCATGTTTA AAGTGGGCAT GTTTAAAATT 
CATCCAGATA TCCCAGAATC TATGTCGTCT GAAGCTAAGG CATTTTTGTT GCGGTGCTTT 
GAACCAGATC CTGTGAAACG CGCTACTGCT ACTGAACTTA TAACGGATGA ATTCTTGAAG 
GTGTCCTCTA GGAAACGCAA GTCCCAGCTT AAGCTTACAA CTCTTTCCTC TGGCTCAAAT 
GAATATCTCC GTAGCATATC TTTGCCGGTG TCAGTGATGG TGGAAGATAC AAGCAGCAGC 
AGTGAATACA GTTCCGTTTC TCCAGATTCA GACTTAAGAT CGGACCCATT TACTTTTAAA 
ACAAGAGCCA GATCATGTAT AGAAAAGGAT GTCAAGCTTA ACAGAAGTTT ATTTCTAGGA 
ATTCCAGACG AGAATTTTGA AGACAGTAGT GCTCCTCCTT CTCCTGAAGA ACGAGACTCT 
GGATTTTTCA TGCTGAAGAA AGATAGTGAA AGGAGGGCTA CACTTTATCA TATTCTTACA 
GAGGATCAGG ACAAGGTGGC TAGAAACTTA ATGGATGCTT TAGTCCAGGG ATCAGAAATA 
AAATTGAAAT GGGACCACAT CACTGCTATT GTTGACAGCC TAAAAGAGTT TGTAAGATCT 
ACTGACAGGA AAATCATTTC AACAACACTA TCAAAACTCA AACTTGAGTT AGACTTTGAT 
AGTCATGCTA TCAGTCAAGT CCAGATGGTG CTGTTTGGCT TTCAAGATGC GGTAAATAAA 
GTGCTACGAA ATCATAACAT AAAGCCTCAC TGGATGTTTG CCTTAGATAA TATAATTCGA 
AAGGCCGTGC AGACAGCTAT CACTATCCTG GTACCTGAGC TGAGGCCACA TTTTAGCATT 
ACATCAGAAA GTGACCAAGC AGATCAGGAG GACATAGAAG TAGAGGAAAG AAGTAATAAA 
CAACCCACAG AACAAACCAT TCACAGAGAA GTCAATGAGG ACAACGTGCA AACCTCAGGA 
GTGAGCACAC TCAGTTCAAC TTTGTCCCAC AACTCTCAGA ATGCCGACAG GTCCCTGAAT 
GTGCAACTTG AAAGACTTAG GTTAGAGTCA AACAGGCTTT TGGAAGAATT GTTACTTAAA 
GAGAGAGAAT TCCAAGTCCT TCTACGCCAA GCTATTGAAG TGAAGGACAG GGAAATCAAA 
CGCCTGCAGT GCACGTCTGA GCCAATAAGT GGGCCCTTGC TAACTGTGGA AGACCACAAT 
GATCCCACAT TCAAAACTCT CTCTTTTCAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819214.1
CDS	186..4118
Translation	MQVMSDSSEGISFSVPCSRLPGSSCCAHEDTGGLTLQENATICGGTRIPEAGSFWREDYLRASAVGSGKDAPCYGKWVNPPPTASGGISLRRITVVYVINEVSPELHACEAAALYSLQEACENHGALLETLDFGKLDFGETSVLDTFYNADIAVVEMNDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIIGQKNTMCTGNYTFIPYMITPQNKVFCCESIFMRGMTELMPSNFELLLGPICLPLVDRFVQILKVAQATSCQYFREAILNDIRRARNQYTGKDLAAELIRIRQRVDSIELLSPDIIINLLLSYRDIQDYDSVVTLVEILEKLPTFNLSSNHHIKFHYAFALNRRNHPGDRVKALEIMLPLVQLEDQVASDIYCIVGRIYKDMFLDSNFMDTESRDNAIHWYRKAFESEPTLHSGINYAVLLLAAGHQFETSLELRKIGVKLSSLLGKKGNLEKLQSYWDVGFYLGACILANDSSKVIHAAEKLFKLKTPAWFLNSVVETILIYQHFKTPNQEQHVTNHELVDFWMDFLVESTSSNVSVLRFPVLILEPTKIYQPSYLSINSEAEVQTISIWHVLPGDKKGIHEWNFPADSIRGVSISKFEERCCFLYVLNNSDDFQIYFCTELHCKKFFDKVNRIKEENGKNTEEGECDGDLMEFDYEYDENGDRVVLGKGKHGVVYAGRDLSNQVRIAIKEVPERDGRCSQPLHEEIALHKRLKHKNIVQYLGSFSKGGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPNTETFTGTLQYMAPEIIDKGPRGYGKPADIWSLGCTVIEMATGKPPFYELGEPQAAMFKVGMFKIHPDIPESMSSEAKAFLLRCFEPDPVKRATATELITDEFLKVSSRKRKSQLKLTTLSSGSNEYLRSISLPVSVMVEDTSSSSEYSSVSPDSDLRSDPFTFKTRARSCIEKDVKLNRSLFLGIPDENFEDSSAPPSPEERDSGFFMLKKDSERRATLYHILTEDQDKVARNLMDALVQGSEIKLKWDHITAIVDSLKEFVRSTDRKIISTTLSKLKLELDFDSHAISQVQMVLFGFQDAVNKVLRNHNIKPHWMFALDNIIRKAVQTAITILVPELRPHFSITSESDQADQEDIEVEERSNKQPTEQTIHREVNEDNVQTSGVSTLSSTLSHNSQNADRSLNVQLERLRLESNRLLEELLLKEREFQVLLRQAIEVKDREIKRLQCTSEPISGPLLTVEDHNDPTFKTLSFQ

Target ORF information:

RefSeq Version	XM_012963760.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963760.3

ORF Insert Sequence:

ATGCAAGTAA TGAGTGACAG CAGCGAAGGA ATCAGTTTCT CTGTACCATG CTCACGGCTG 
CCTGGCTCAA GCTGCTGTGC TCATGAAGAC ACAGGAGGGC TTACGCTACA GGAAAATGCC 
ACCATATGCG GTGGAACCAG GATCCCTGAA GCTGGAAGCT TCTGGCGTGA AGATTACCTC 
CGTGCTTCAG CGGTTGGAAG TGGTAAAGAT GCCCCATGCT ATGGAAAGTG GGTTAACCCC 
CCTCCTACAG CTAGCGGAGG TATTAGCCTT CGTAGGATTA CTGTGGTATA TGTTATCAAT 
GAAGTAAGTC CGGAGTTGCA TGCTTGTGAG GCTGCTGCAT TATACAGTTT GCAGGAGGCA 
TGTGAGAACC ATGGGGCACT GCTTGAGACC CTGGATTTCG GCAAACTGGA CTTTGGCGAG 
ACGTCAGTGC TAGACACCTT CTACAATGCA GATATTGCAG TGGTTGAGAT GAATGACGCT 
TTTCGACAGC CTTCTTTGTT CTACCATCTG GGAGTAAGAG AAAGCTTTAG CATGGCAAAC 
AACATAATTC TTTACTGTGA CACAAATTCA GACTCCCTGC AGTCACTGAA GGAAATTATC 
GGGCAAAAGA ATACTATGTG CACAGGTAAT TACACATTTA TTCCTTACAT GATCACGCCA 
CAAAACAAAG TGTTTTGCTG TGAGAGTATT TTCATGAGAG GAATGACTGA ACTAATGCCG 
TCAAACTTTG AGTTACTTCT TGGTCCGATC TGCCTGCCCC TAGTGGATCG CTTTGTTCAG 
ATTCTGAAGG TGGCACAGGC CACGTCATGC CAATATTTTC GAGAGGCAAT ACTAAATGAT 
ATTAGAAGGG CTCGGAACCA ATACACTGGG AAGGATCTGG CTGCTGAGCT AATAAGGATC 
CGCCAGCGCG TAGACAGCAT CGAGTTGTTA TCACCTGACA TCATTATCAA TCTGCTTTTG 
TCATACAGAG ATATCCAGGA CTACGATTCA GTTGTGACGT TGGTGGAAAT ATTGGAGAAA 
CTTCCAACCT TTAATTTATC TTCCAATCAC CATATAAAAT TTCATTATGC ATTTGCATTA 
AACAGGAGAA ATCATCCAGG TGACAGAGTA AAAGCCCTTG AAATAATGTT GCCTTTGGTG 
CAACTTGAAG ATCAAGTAGC TTCAGACATA TACTGCATTG TGGGAAGGAT CTACAAAGAT 
ATGTTCTTAG ATTCTAACTT CATGGATACC GAAAGTCGTG ATAATGCTAT ACATTGGTAC 
AGGAAGGCCT TTGAATCTGA ACCAACACTT CATTCTGGAA TCAATTATGC GGTGCTACTT 
CTAGCTGCTG GTCATCAATT TGAGACTTCT TTGGAACTCC GGAAAATCGG AGTTAAACTC 
AGTAGCCTGT TGGGTAAAAA AGGAAATCTC GAAAAACTCC AAAGTTATTG GGATGTTGGA 
TTTTATTTGG GGGCATGTAT ACTGGCCAAT GACTCATCAA AAGTTATCCA TGCAGCAGAA 
AAGCTTTTCA AGCTCAAAAC CCCAGCATGG TTCTTGAATT CAGTTGTGGA GACCATTTTG 
ATTTATCAGC ATTTTAAAAC GCCAAACCAA GAACAACATG TAACAAACCA TGAGCTGGTT 
GATTTTTGGA TGGATTTTCT TGTTGAGTCT ACAAGTAGCA ATGTGTCTGT ATTACGGTTT 
CCTGTCTTAA TACTAGAGCC AACAAAAATC TACCAGCCTT CCTACCTATC AATCAATAGT 
GAAGCTGAAG TTCAGACAAT ATCTATTTGG CACGTTCTAC CTGGCGATAA GAAAGGAATC 
CATGAATGGA ATTTCCCTGC AGATTCTATC AGAGGTGTAA GCATTTCAAA GTTTGAAGAA 
AGGTGCTGTT TTCTGTATGT TCTGAACAAC TCTGACGATT TTCAAATATA TTTCTGCACA 
GAGCTCCACT GCAAAAAATT CTTTGATAAA GTAAACCGTA TCAAAGAAGA GAATGGAAAG 
AACACAGAAG AAGGGGAGTG TGATGGAGAT TTAATGGAGT TCGACTATGA GTATGATGAA 
AATGGGGACA GAGTGGTTCT CGGTAAAGGG AAACATGGAG TGGTATATGC AGGAAGAGAC 
TTGAGTAACC AGGTCAGAAT TGCAATTAAA GAAGTTCCAG AGAGAGATGG TCGATGCTCT 
CAACCACTTC ATGAAGAGAT TGCTTTGCAT AAACGTCTAA AGCATAAAAA CATTGTTCAG 
TATTTGGGAT CATTCAGTAA AGGTGGTTTC ATTAAAATAT TTATGGAACA AGTACCTGGA 
GGAAGCCTCT CCGCACTTCT AAGGTCAAAA TGGGGACCCT TGAAAGACAA TGAGCAGACA 
ATCGGATTTT ATACCAAACA GATCCTTGAA GGTTTGAAAT ATCTCCATGA TAACCAGATA 
GTTCATAGAG ATATCAAGGG TGATAATGTT CTTATAAACA CATACAGTGG TGTTCTGAAA 
ATCTCTGATT TTGGCACTTC AAAGAGACTT GCTGGAATCA ACCCAAATAC AGAAACCTTC 
ACTGGCACTC TACAATATAT GGCACCAGAA ATTATAGACA AAGGACCAAG AGGATATGGA 
AAGCCAGCAG ACATTTGGTC TCTGGGATGC ACAGTTATAG AAATGGCAAC AGGAAAACCA 
CCATTTTATG AGCTTGGAGA ACCACAAGCT GCCATGTTTA AAGTGGGCAT GTTTAAAATT 
CATCCAGATA TCCCAGAATC TATGTCGTCT GAAGCTAAGG CATTTTTGTT GCGGTGCTTT 
GAACCAGATC CTGTGAAACG CGCTACTGCT ACTGAACTTA TAACGGATGA ATTCTTGAAG 
GTGTCCTCTA GGAAACGCAA GTCCCAGCTT AAGCTTACAA CTCTTTCCTC TGGCTCAAAT 
GAATATCTCC GTAGCATATC TTTGCCGGTG TCAGTGATGG TGGAAGATAC AAGCAGCAGC 
AGTGAATACA GTTCCGTTTC TCCAGATTCA GACTTAAGAT CGGACCCATT TACTTTTAAA 
ACAAGAGCCA GATCATGTAT AGAAAAGGAT GTCAAGCTTA ACAGAAGTTT ATTTCTAGGA 
ATTCCAGACG AGAATTTTGA AGACAGTAGT GCTCCTCCTT CTCCTGAAGA ACGAGACTCT 
GGATTTTTCA TGCTGAAGAA AGATAGTGAA AGGAGGGCTA CACTTTATCA TATTCTTACA 
GAGGATCAGG ACAAGGTGGC TAGAAACTTA ATGGATGCTT TAGTCCAGGG ATCAGAAATA 
AAATTGAAAT GGGACCACAT CACTGCTATT GTTGACAGCC TAAAAGAGTT TGTAAGATCT 
ACTGACAGGA AAATCATTTC AACAACACTA TCAAAACTCA AACTTGAGTT AGACTTTGAT 
AGTCATGCTA TCAGTCAAGT CCAGATGGTG CTGTTTGGCT TTCAAGATGC GGTAAATAAA 
GTGCTACGAA ATCATAACAT AAAGCCTCAC TGGATGTTTG CCTTAGATAA TATAATTCGA 
AAGGCCGTGC AGACAGCTAT CACTATCCTG GTACCTGAGC TGAGGCCACA TTTTAGCATT 
ACATCAGAAA GTGACCAAGC AGATCAGGAG GACATAGAAG TAGAGGAAAG AAGTAATAAA 
CAACCCACAG AACAAACCAT TCACAGAGAA GTCAATGAGG ACAACGTGCA AACCTCAGGA 
GTGAGCACAC TCAGTTCAAC TTTGTCCCAC AACTCTCAGA ATGCCGACAG GTCCCTGAAT 
GTGCAACTTG AAAGACTTAG GTTAGAGTCA AACAGGCTTT TGGAAGAATT GTTACTTAAA 
GAGAGAGAAT TCCAAGTCCT TCTACGCCAA GCTATTGAAG TGAAGGACAG GGAAATCAAA 
CGCCTGCAGT GCACGTCTGA GCCAATAAGT GGGCCCTTGC TAACTGTGGA AGACCACAAT 
GATCCCACAT TCAAAACTCT CTCTTTTCAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k5 ( XM_012963762.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819216.1 Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa36968
Clone ID Related Accession (Same CDS sequence)	XM_012963762.2 , XM_012963762.3
Accession Version	XM_012963762.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963762.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTAA TGAGTGACAG CAGCGAAGGA ATCAGTTTCT CTGTACCATG CTCACGGCTG 
CCTGGCTCAA GCTGCTGTGC TCATGAAGAC ACAGGAGGGC TTACGCTACA GGAAAATGCC 
ACCATATGCG GTGGAACCAG GATCCCTGAA GCTGGAAGCT TCTGGCGTGA AGATTACCTC 
CGTGCTTCAG CGGTTGGAAG TGGTAAAGAT GCCCCATGCT ATGGAAAGTG GGTTAACCCC 
CCTCCTACAG CTAGCGGAGG TATTAGCCTT CGTAGGATTA CTGTGGTATA TGTTATCAAT 
GAAGTAAGTC CGGAGTTGCA TGCTTGTGAG GCTGCTGCAT TATACAGTTT GCAGGAGGCA 
TGTGAGAACC ATGGGGCACT GCTTGAGACC CTGGATTTCG GCAAACTGGA CTTTGGCGAG 
ACGTCAGTGC TAGACACCTT CTACAATGCA GATATTGCAG TGGTTGAGAT GAATGACGCT 
TTTCGACAGC CTTCTTTGTT CTACCATCTG GGAGTAAGAG AAAGCTTTAG CATGGCAAAC 
AACATAATTC TTTACTGTGA CACAAATTCA GACTCCCTGC AGTCACTGAA GATGTGCACA 
GGTAATTACA CATTTATTCC TTACATGATC ACGCCACAAA ACAAAGTGTT TTGCTGTGAG 
AGTATTTTCA TGAGAGGAAT GACTGAACTA ATGCCGTCAA ACTTTGAGTT ACTTCTTGGT 
CCGATCTGCC TGCCCCTAGT GGATCGCTTT GTTCAGATTC TGAAGGTGGC ACAGGCCACG 
TCATGCCAAT ATTTTCGAGA GGCAATACTA AATGATATTA GAAGGGCTCG GAACCAATAC 
ACTGGGAAGG ATCTGGCTGC TGAGCTAATA AGGATCCGCC AGCGCGTAGA CAGCATCGAG 
TTGTTATCAC CTGACATCAT TATCAATCTG CTTTTGTCAT ACAGAGATAT CCAGGACTAC 
GATTCAGTTG TGACGTTGGT GGAAATATTG GAGAAACTTC CAACCTTTAA TTTATCTTCC 
AATCACCATA TAAAATTTCA TTATGCATTT GCATTAAACA GGAGAAATCA TCCAGGTGAC 
AGAGTAAAAG CCCTTGAAAT AATGTTGCCT TTGGTGCAAC TTGAAGATCA AGTAGCTTCA 
GACATATACT GCATTGTGGG AAGGATCTAC AAAGATATGT TCTTAGATTC TAACTTCATG 
GATACCGAAA GTCGTGATAA TGCTATACAT TGGTACAGGA AGGCCTTTGA ATCTGAACCA 
ACACTTCATT CTGGAATCAA TTATGCGGTG CTACTTCTAG CTGCTGGTCA TCAATTTGAG 
ACTTCTTTGG AACTCCGGAA AATCGGAGTT AAACTCAGTA GCCTGTTGGG TAAAAAAGGA 
AATCTCGAAA AACTCCAAAG TTATTGGGAT GTTGGATTTT ATTTGGGGGC ATGTATACTG 
GCCAATGACT CATCAAAAGT TATCCATGCA GCAGAAAAGC TTTTCAAGCT CAAAACCCCA 
GCATGGTTCT TGAATTCAGT TGTGGAGACC ATTTTGATTT ATCAGCATTT TAAAACGCCA 
AACCAAGAAC AACATGTAAC AAACCATGAG CTGGTTGATT TTTGGATGGA TTTTCTTGTT 
GAGTCTACAA GTAGCAATGT GTCTGTATTA CGGTTTCCTG TCTTAATACT AGAGCCAACA 
AAAATCTACC AGCCTTCCTA CCTATCAATC AATAGTGAAG CTGAAGTTCA GACAATATCT 
ATTTGGCACG TTCTACCTGG CGATAAGAAA GGAATCCATG AATGGAATTT CCCTGCAGAT 
TCTATCAGAG GTGTAAGCAT TTCAAAGTTT GAAGAAAGGT GCTGTTTTCT GTATGTTCTG 
AACAACTCTG ACGATTTTCA AATATATTTC TGCACAGAGC TCCACTGCAA AAAATTCTTT 
GATAAAGTAA ACCGTATCAA AGAAGAGAAT GGAAAGAACA CAGAAGAAGG GGAGTGTGAT 
GGAGATTTAA TGGAGTTCGA CTATGAGTAT GATGAAAATG GGGACAGAGT GGTTCTCGGT 
AAAGGGAAAC ATGGAGTGGT ATATGCAGGA AGAGACTTGA GTAACCAGGT CAGAATTGCA 
ATTAAAGAAG TTCCAGAGAG AGATGGTCGA TGCTCTCAAC CACTTCATGA AGAGATTGCT 
TTGCATAAAC GTCTAAAGCA TAAAAACATT GTTCAGTATT TGGGATCATT CAGTAAAGGT 
GGTTTCATTA AAATATTTAT GGAACAAGTA CCTGGAGGAA GCCTCTCCGC ACTTCTAAGG 
TCAAAATGGG GACCCTTGAA AGACAATGAG CAGACAATCG GATTTTATAC CAAACAGATC 
CTTGAAGGTT TGAAATATCT CCATGATAAC CAGATAGTTC ATAGAGATAT CAAGGGTGAT 
AATGTTCTTA TAAACACATA CAGTGGTGTT CTGAAAATCT CTGATTTTGG CACTTCAAAG 
AGACTTGCTG GAATCAACCC AAATACAGAA ACCTTCACTG GCACTCTACA ATATATGGCA 
CCAGAAATTA TAGACAAAGG ACCAAGAGGA TATGGAAAGC CAGCAGACAT TTGGTCTCTG 
GGATGCACAG TTATAGAAAT GGCAACAGGA AAACCACCAT TTTATGAGCT TGGAGAACCA 
CAAGCTGCCA TGTTTAAAGT GGGCATGTTT AAAATTCATC CAGATATCCC AGAATCTATG 
TCGTCTGAAG CTAAGGCATT TTTGTTGCGG TGCTTTGAAC CAGATCCTGT GAAACGCGCT 
ACTGCTACTG AACTTATAAC GGATGAATTC TTGAAGGTGT CCTCTAGGAA ACGCAAGTCC 
CAGCTTAAGC TTACAACTCT TTCCTCTGGC TCAAATGAAT ATCTCCGTAG CATATCTTTG 
CCGGTGTCAG TGATGGTGGA AGATACAAGC AGCAGCAGTG AATACAGTTC CGTTTCTCCA 
GATTCAGACT TAAGATCGGA CCCATTTACT TTTAAAACAA GAGCCAGATC ATGTATAGAA 
AAGGATGTCA AGCTTAACAG AAGTTTATTT CTAGGAATTC CAGACGAGAA TTTTGAAGAC 
AGTAGTGCTC CTCCTTCTCC TGAAGAACGA GACTCTGGAT TTTTCATGCT GAAGAAAGAT 
AGTGAAAGGA GGGCTACACT TTATCATATT CTTACAGAGG ATCAGGACAA GGTGGCTAGA 
AACTTAATGG ATGCTTTAGT CCAGGGATCA GAAATAAAAT TGAAATGGGA CCACATCACT 
GCTATTGTTG ACAGCCTAAA AGAGTTTGTA AGATCTACTG ACAGGAAAAT CATTTCAACA 
ACACTATCAA AACTCAAACT TGAGTTAGAC TTTGATAGTC ATGCTATCAG TCAAGTCCAG 
ATGGTGCTGT TTGGCTTTCA AGATGCGGTA AATAAAGTGC TACGAAATCA TAACATAAAG 
CCTCACTGGA TGTTTGCCTT AGATAATATA ATTCGAAAGG CCGTGCAGAC AGCTATCACT 
ATCCTGGTAC CTGAGCTGAG GCCACATTTT AGCATTACAT CAGAAAGTGA CCAAGCAGAT 
CAGGAGGACA TAGAAGTAGA GGAAAGAAGT AATAAACAAC CCACAGAACA AACCATTCAC 
AGAGAAGTCA ATGAGGACAA CGTGCAAACC TCAGGAGTGA GCACACTCAG TTCAACTTTG 
TCCCACAACT CTCAGAATGC CGACAGGTCC CTGAATGTGC AACTTGAAAG ACTTAGGTTA 
GAGTCAAACA GGCTTTTGGA AGAATTGTTA CTTAAAGAGA GAGAATTCCA AGTCCTTCTA 
CGCCAAGCTA TTGAAGTGAA GGACAGGGAA ATCAAACGCC TGCAGTGCAC GTCTGAGCCA 
ATAAGCACTT CCGTATCCAG CACAAGGTCA ACTATCTCAG GCAGTGAAGA CTTGAATCTT 
GTTTACTGGC TACACAAGTG CAGAGTAGAT GAAGATACAA TAAGCAAGTT TTTGGCAGAA 
GACTATCAGC TTGAAGATAT TCTTTGTCAT GTCACACGAG ATGATTTAAA ATCTCTGAAT 
CTGAGGGGAG GAGTACTATG CACAATATGG AAAGAAATCA CTGACTACAG AGAAAAACAA 
GCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819216.1
CDS	185..4273
Translation	MQVMSDSSEGISFSVPCSRLPGSSCCAHEDTGGLTLQENATICGGTRIPEAGSFWREDYLRASAVGSGKDAPCYGKWVNPPPTASGGISLRRITVVYVINEVSPELHACEAAALYSLQEACENHGALLETLDFGKLDFGETSVLDTFYNADIAVVEMNDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKMCTGNYTFIPYMITPQNKVFCCESIFMRGMTELMPSNFELLLGPICLPLVDRFVQILKVAQATSCQYFREAILNDIRRARNQYTGKDLAAELIRIRQRVDSIELLSPDIIINLLLSYRDIQDYDSVVTLVEILEKLPTFNLSSNHHIKFHYAFALNRRNHPGDRVKALEIMLPLVQLEDQVASDIYCIVGRIYKDMFLDSNFMDTESRDNAIHWYRKAFESEPTLHSGINYAVLLLAAGHQFETSLELRKIGVKLSSLLGKKGNLEKLQSYWDVGFYLGACILANDSSKVIHAAEKLFKLKTPAWFLNSVVETILIYQHFKTPNQEQHVTNHELVDFWMDFLVESTSSNVSVLRFPVLILEPTKIYQPSYLSINSEAEVQTISIWHVLPGDKKGIHEWNFPADSIRGVSISKFEERCCFLYVLNNSDDFQIYFCTELHCKKFFDKVNRIKEENGKNTEEGECDGDLMEFDYEYDENGDRVVLGKGKHGVVYAGRDLSNQVRIAIKEVPERDGRCSQPLHEEIALHKRLKHKNIVQYLGSFSKGGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPNTETFTGTLQYMAPEIIDKGPRGYGKPADIWSLGCTVIEMATGKPPFYELGEPQAAMFKVGMFKIHPDIPESMSSEAKAFLLRCFEPDPVKRATATELITDEFLKVSSRKRKSQLKLTTLSSGSNEYLRSISLPVSVMVEDTSSSSEYSSVSPDSDLRSDPFTFKTRARSCIEKDVKLNRSLFLGIPDENFEDSSAPPSPEERDSGFFMLKKDSERRATLYHILTEDQDKVARNLMDALVQGSEIKLKWDHITAIVDSLKEFVRSTDRKIISTTLSKLKLELDFDSHAISQVQMVLFGFQDAVNKVLRNHNIKPHWMFALDNIIRKAVQTAITILVPELRPHFSITSESDQADQEDIEVEERSNKQPTEQTIHREVNEDNVQTSGVSTLSSTLSHNSQNADRSLNVQLERLRLESNRLLEELLLKEREFQVLLRQAIEVKDREIKRLQCTSEPISTSVSSTRSTISGSEDLNLVYWLHKCRVDEDTISKFLAEDYQLEDILCHVTRDDLKSLNLRGGVLCTIWKEITDYREKQAS

Target ORF information:

RefSeq Version	XM_012963762.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963762.3

ORF Insert Sequence:

ATGCAAGTAA TGAGTGACAG CAGCGAAGGA ATCAGTTTCT CTGTACCATG CTCACGGCTG 
CCTGGCTCAA GCTGCTGTGC TCATGAAGAC ACAGGAGGGC TTACGCTACA GGAAAATGCC 
ACCATATGCG GTGGAACCAG GATCCCTGAA GCTGGAAGCT TCTGGCGTGA AGATTACCTC 
CGTGCTTCAG CGGTTGGAAG TGGTAAAGAT GCCCCATGCT ATGGAAAGTG GGTTAACCCC 
CCTCCTACAG CTAGCGGAGG TATTAGCCTT CGTAGGATTA CTGTGGTATA TGTTATCAAT 
GAAGTAAGTC CGGAGTTGCA TGCTTGTGAG GCTGCTGCAT TATACAGTTT GCAGGAGGCA 
TGTGAGAACC ATGGGGCACT GCTTGAGACC CTGGATTTCG GCAAACTGGA CTTTGGCGAG 
ACGTCAGTGC TAGACACCTT CTACAATGCA GATATTGCAG TGGTTGAGAT GAATGACGCT 
TTTCGACAGC CTTCTTTGTT CTACCATCTG GGAGTAAGAG AAAGCTTTAG CATGGCAAAC 
AACATAATTC TTTACTGTGA CACAAATTCA GACTCCCTGC AGTCACTGAA GATGTGCACA 
GGTAATTACA CATTTATTCC TTACATGATC ACGCCACAAA ACAAAGTGTT TTGCTGTGAG 
AGTATTTTCA TGAGAGGAAT GACTGAACTA ATGCCGTCAA ACTTTGAGTT ACTTCTTGGT 
CCGATCTGCC TGCCCCTAGT GGATCGCTTT GTTCAGATTC TGAAGGTGGC ACAGGCCACG 
TCATGCCAAT ATTTTCGAGA GGCAATACTA AATGATATTA GAAGGGCTCG GAACCAATAC 
ACTGGGAAGG ATCTGGCTGC TGAGCTAATA AGGATCCGCC AGCGCGTAGA CAGCATCGAG 
TTGTTATCAC CTGACATCAT TATCAATCTG CTTTTGTCAT ACAGAGATAT CCAGGACTAC 
GATTCAGTTG TGACGTTGGT GGAAATATTG GAGAAACTTC CAACCTTTAA TTTATCTTCC 
AATCACCATA TAAAATTTCA TTATGCATTT GCATTAAACA GGAGAAATCA TCCAGGTGAC 
AGAGTAAAAG CCCTTGAAAT AATGTTGCCT TTGGTGCAAC TTGAAGATCA AGTAGCTTCA 
GACATATACT GCATTGTGGG AAGGATCTAC AAAGATATGT TCTTAGATTC TAACTTCATG 
GATACCGAAA GTCGTGATAA TGCTATACAT TGGTACAGGA AGGCCTTTGA ATCTGAACCA 
ACACTTCATT CTGGAATCAA TTATGCGGTG CTACTTCTAG CTGCTGGTCA TCAATTTGAG 
ACTTCTTTGG AACTCCGGAA AATCGGAGTT AAACTCAGTA GCCTGTTGGG TAAAAAAGGA 
AATCTCGAAA AACTCCAAAG TTATTGGGAT GTTGGATTTT ATTTGGGGGC ATGTATACTG 
GCCAATGACT CATCAAAAGT TATCCATGCA GCAGAAAAGC TTTTCAAGCT CAAAACCCCA 
GCATGGTTCT TGAATTCAGT TGTGGAGACC ATTTTGATTT ATCAGCATTT TAAAACGCCA 
AACCAAGAAC AACATGTAAC AAACCATGAG CTGGTTGATT TTTGGATGGA TTTTCTTGTT 
GAGTCTACAA GTAGCAATGT GTCTGTATTA CGGTTTCCTG TCTTAATACT AGAGCCAACA 
AAAATCTACC AGCCTTCCTA CCTATCAATC AATAGTGAAG CTGAAGTTCA GACAATATCT 
ATTTGGCACG TTCTACCTGG CGATAAGAAA GGAATCCATG AATGGAATTT CCCTGCAGAT 
TCTATCAGAG GTGTAAGCAT TTCAAAGTTT GAAGAAAGGT GCTGTTTTCT GTATGTTCTG 
AACAACTCTG ACGATTTTCA AATATATTTC TGCACAGAGC TCCACTGCAA AAAATTCTTT 
GATAAAGTAA ACCGTATCAA AGAAGAGAAT GGAAAGAACA CAGAAGAAGG GGAGTGTGAT 
GGAGATTTAA TGGAGTTCGA CTATGAGTAT GATGAAAATG GGGACAGAGT GGTTCTCGGT 
AAAGGGAAAC ATGGAGTGGT ATATGCAGGA AGAGACTTGA GTAACCAGGT CAGAATTGCA 
ATTAAAGAAG TTCCAGAGAG AGATGGTCGA TGCTCTCAAC CACTTCATGA AGAGATTGCT 
TTGCATAAAC GTCTAAAGCA TAAAAACATT GTTCAGTATT TGGGATCATT CAGTAAAGGT 
GGTTTCATTA AAATATTTAT GGAACAAGTA CCTGGAGGAA GCCTCTCCGC ACTTCTAAGG 
TCAAAATGGG GACCCTTGAA AGACAATGAG CAGACAATCG GATTTTATAC CAAACAGATC 
CTTGAAGGTT TGAAATATCT CCATGATAAC CAGATAGTTC ATAGAGATAT CAAGGGTGAT 
AATGTTCTTA TAAACACATA CAGTGGTGTT CTGAAAATCT CTGATTTTGG CACTTCAAAG 
AGACTTGCTG GAATCAACCC AAATACAGAA ACCTTCACTG GCACTCTACA ATATATGGCA 
CCAGAAATTA TAGACAAAGG ACCAAGAGGA TATGGAAAGC CAGCAGACAT TTGGTCTCTG 
GGATGCACAG TTATAGAAAT GGCAACAGGA AAACCACCAT TTTATGAGCT TGGAGAACCA 
CAAGCTGCCA TGTTTAAAGT GGGCATGTTT AAAATTCATC CAGATATCCC AGAATCTATG 
TCGTCTGAAG CTAAGGCATT TTTGTTGCGG TGCTTTGAAC CAGATCCTGT GAAACGCGCT 
ACTGCTACTG AACTTATAAC GGATGAATTC TTGAAGGTGT CCTCTAGGAA ACGCAAGTCC 
CAGCTTAAGC TTACAACTCT TTCCTCTGGC TCAAATGAAT ATCTCCGTAG CATATCTTTG 
CCGGTGTCAG TGATGGTGGA AGATACAAGC AGCAGCAGTG AATACAGTTC CGTTTCTCCA 
GATTCAGACT TAAGATCGGA CCCATTTACT TTTAAAACAA GAGCCAGATC ATGTATAGAA 
AAGGATGTCA AGCTTAACAG AAGTTTATTT CTAGGAATTC CAGACGAGAA TTTTGAAGAC 
AGTAGTGCTC CTCCTTCTCC TGAAGAACGA GACTCTGGAT TTTTCATGCT GAAGAAAGAT 
AGTGAAAGGA GGGCTACACT TTATCATATT CTTACAGAGG ATCAGGACAA GGTGGCTAGA 
AACTTAATGG ATGCTTTAGT CCAGGGATCA GAAATAAAAT TGAAATGGGA CCACATCACT 
GCTATTGTTG ACAGCCTAAA AGAGTTTGTA AGATCTACTG ACAGGAAAAT CATTTCAACA 
ACACTATCAA AACTCAAACT TGAGTTAGAC TTTGATAGTC ATGCTATCAG TCAAGTCCAG 
ATGGTGCTGT TTGGCTTTCA AGATGCGGTA AATAAAGTGC TACGAAATCA TAACATAAAG 
CCTCACTGGA TGTTTGCCTT AGATAATATA ATTCGAAAGG CCGTGCAGAC AGCTATCACT 
ATCCTGGTAC CTGAGCTGAG GCCACATTTT AGCATTACAT CAGAAAGTGA CCAAGCAGAT 
CAGGAGGACA TAGAAGTAGA GGAAAGAAGT AATAAACAAC CCACAGAACA AACCATTCAC 
AGAGAAGTCA ATGAGGACAA CGTGCAAACC TCAGGAGTGA GCACACTCAG TTCAACTTTG 
TCCCACAACT CTCAGAATGC CGACAGGTCC CTGAATGTGC AACTTGAAAG ACTTAGGTTA 
GAGTCAAACA GGCTTTTGGA AGAATTGTTA CTTAAAGAGA GAGAATTCCA AGTCCTTCTA 
CGCCAAGCTA TTGAAGTGAA GGACAGGGAA ATCAAACGCC TGCAGTGCAC GTCTGAGCCA 
ATAAGCACTT CCGTATCCAG CACAAGGTCA ACTATCTCAG GCAGTGAAGA CTTGAATCTT 
GTTTACTGGC TACACAAGTG CAGAGTAGAT GAAGATACAA TAAGCAAGTT TTTGGCAGAA 
GACTATCAGC TTGAAGATAT TCTTTGTCAT GTCACACGAG ATGATTTAAA ATCTCTGAAT 
CTGAGGGGAG GAGTACTATG CACAATATGG AAAGAAATCA CTGACTACAG AGAAAAACAA 
GCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k5 ( XM_012963760.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819214.1 Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa36966
Clone ID Related Accession (Same CDS sequence)	XM_012963760.2 , XM_012963760.3
Accession Version	XM_012963760.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963760.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTAA TGAGTGACAG CAGCGAAGGA ATCAGTTTCT CTGTACCATG CTCACGGCTG 
CCTGGCTCAA GCTGCTGTGC TCATGAAGAC ACAGGAGGGC TTACGCTACA GGAAAATGCC 
ACCATATGCG GTGGAACCAG GATCCCTGAA GCTGGAAGCT TCTGGCGTGA AGATTACCTC 
CGTGCTTCAG CGGTTGGAAG TGGTAAAGAT GCCCCATGCT ATGGAAAGTG GGTTAACCCC 
CCTCCTACAG CTAGCGGAGG TATTAGCCTT CGTAGGATTA CTGTGGTATA TGTTATCAAT 
GAAGTAAGTC CGGAGTTGCA TGCTTGTGAG GCTGCTGCAT TATACAGTTT GCAGGAGGCA 
TGTGAGAACC ATGGGGCACT GCTTGAGACC CTGGATTTCG GCAAACTGGA CTTTGGCGAG 
ACGTCAGTGC TAGACACCTT CTACAATGCA GATATTGCAG TGGTTGAGAT GAATGACGCT 
TTTCGACAGC CTTCTTTGTT CTACCATCTG GGAGTAAGAG AAAGCTTTAG CATGGCAAAC 
AACATAATTC TTTACTGTGA CACAAATTCA GACTCCCTGC AGTCACTGAA GGAAATTATC 
GGGCAAAAGA ATACTATGTG CACAGGTAAT TACACATTTA TTCCTTACAT GATCACGCCA 
CAAAACAAAG TGTTTTGCTG TGAGAGTATT TTCATGAGAG GAATGACTGA ACTAATGCCG 
TCAAACTTTG AGTTACTTCT TGGTCCGATC TGCCTGCCCC TAGTGGATCG CTTTGTTCAG 
ATTCTGAAGG TGGCACAGGC CACGTCATGC CAATATTTTC GAGAGGCAAT ACTAAATGAT 
ATTAGAAGGG CTCGGAACCA ATACACTGGG AAGGATCTGG CTGCTGAGCT AATAAGGATC 
CGCCAGCGCG TAGACAGCAT CGAGTTGTTA TCACCTGACA TCATTATCAA TCTGCTTTTG 
TCATACAGAG ATATCCAGGA CTACGATTCA GTTGTGACGT TGGTGGAAAT ATTGGAGAAA 
CTTCCAACCT TTAATTTATC TTCCAATCAC CATATAAAAT TTCATTATGC ATTTGCATTA 
AACAGGAGAA ATCATCCAGG TGACAGAGTA AAAGCCCTTG AAATAATGTT GCCTTTGGTG 
CAACTTGAAG ATCAAGTAGC TTCAGACATA TACTGCATTG TGGGAAGGAT CTACAAAGAT 
ATGTTCTTAG ATTCTAACTT CATGGATACC GAAAGTCGTG ATAATGCTAT ACATTGGTAC 
AGGAAGGCCT TTGAATCTGA ACCAACACTT CATTCTGGAA TCAATTATGC GGTGCTACTT 
CTAGCTGCTG GTCATCAATT TGAGACTTCT TTGGAACTCC GGAAAATCGG AGTTAAACTC 
AGTAGCCTGT TGGGTAAAAA AGGAAATCTC GAAAAACTCC AAAGTTATTG GGATGTTGGA 
TTTTATTTGG GGGCATGTAT ACTGGCCAAT GACTCATCAA AAGTTATCCA TGCAGCAGAA 
AAGCTTTTCA AGCTCAAAAC CCCAGCATGG TTCTTGAATT CAGTTGTGGA GACCATTTTG 
ATTTATCAGC ATTTTAAAAC GCCAAACCAA GAACAACATG TAACAAACCA TGAGCTGGTT 
GATTTTTGGA TGGATTTTCT TGTTGAGTCT ACAAGTAGCA ATGTGTCTGT ATTACGGTTT 
CCTGTCTTAA TACTAGAGCC AACAAAAATC TACCAGCCTT CCTACCTATC AATCAATAGT 
GAAGCTGAAG TTCAGACAAT ATCTATTTGG CACGTTCTAC CTGGCGATAA GAAAGGAATC 
CATGAATGGA ATTTCCCTGC AGATTCTATC AGAGGTGTAA GCATTTCAAA GTTTGAAGAA 
AGGTGCTGTT TTCTGTATGT TCTGAACAAC TCTGACGATT TTCAAATATA TTTCTGCACA 
GAGCTCCACT GCAAAAAATT CTTTGATAAA GTAAACCGTA TCAAAGAAGA GAATGGAAAG 
AACACAGAAG AAGGGGAGTG TGATGGAGAT TTAATGGAGT TCGACTATGA GTATGATGAA 
AATGGGGACA GAGTGGTTCT CGGTAAAGGG AAACATGGAG TGGTATATGC AGGAAGAGAC 
TTGAGTAACC AGGTCAGAAT TGCAATTAAA GAAGTTCCAG AGAGAGATGG TCGATGCTCT 
CAACCACTTC ATGAAGAGAT TGCTTTGCAT AAACGTCTAA AGCATAAAAA CATTGTTCAG 
TATTTGGGAT CATTCAGTAA AGGTGGTTTC ATTAAAATAT TTATGGAACA AGTACCTGGA 
GGAAGCCTCT CCGCACTTCT AAGGTCAAAA TGGGGACCCT TGAAAGACAA TGAGCAGACA 
ATCGGATTTT ATACCAAACA GATCCTTGAA GGTTTGAAAT ATCTCCATGA TAACCAGATA 
GTTCATAGAG ATATCAAGGG TGATAATGTT CTTATAAACA CATACAGTGG TGTTCTGAAA 
ATCTCTGATT TTGGCACTTC AAAGAGACTT GCTGGAATCA ACCCAAATAC AGAAACCTTC 
ACTGGCACTC TACAATATAT GGCACCAGAA ATTATAGACA AAGGACCAAG AGGATATGGA 
AAGCCAGCAG ACATTTGGTC TCTGGGATGC ACAGTTATAG AAATGGCAAC AGGAAAACCA 
CCATTTTATG AGCTTGGAGA ACCACAAGCT GCCATGTTTA AAGTGGGCAT GTTTAAAATT 
CATCCAGATA TCCCAGAATC TATGTCGTCT GAAGCTAAGG CATTTTTGTT GCGGTGCTTT 
GAACCAGATC CTGTGAAACG CGCTACTGCT ACTGAACTTA TAACGGATGA ATTCTTGAAG 
GTGTCCTCTA GGAAACGCAA GTCCCAGCTT AAGCTTACAA CTCTTTCCTC TGGCTCAAAT 
GAATATCTCC GTAGCATATC TTTGCCGGTG TCAGTGATGG TGGAAGATAC AAGCAGCAGC 
AGTGAATACA GTTCCGTTTC TCCAGATTCA GACTTAAGAT CGGACCCATT TACTTTTAAA 
ACAAGAGCCA GATCATGTAT AGAAAAGGAT GTCAAGCTTA ACAGAAGTTT ATTTCTAGGA 
ATTCCAGACG AGAATTTTGA AGACAGTAGT GCTCCTCCTT CTCCTGAAGA ACGAGACTCT 
GGATTTTTCA TGCTGAAGAA AGATAGTGAA AGGAGGGCTA CACTTTATCA TATTCTTACA 
GAGGATCAGG ACAAGGTGGC TAGAAACTTA ATGGATGCTT TAGTCCAGGG ATCAGAAATA 
AAATTGAAAT GGGACCACAT CACTGCTATT GTTGACAGCC TAAAAGAGTT TGTAAGATCT 
ACTGACAGGA AAATCATTTC AACAACACTA TCAAAACTCA AACTTGAGTT AGACTTTGAT 
AGTCATGCTA TCAGTCAAGT CCAGATGGTG CTGTTTGGCT TTCAAGATGC GGTAAATAAA 
GTGCTACGAA ATCATAACAT AAAGCCTCAC TGGATGTTTG CCTTAGATAA TATAATTCGA 
AAGGCCGTGC AGACAGCTAT CACTATCCTG GTACCTGAGC TGAGGCCACA TTTTAGCATT 
ACATCAGAAA GTGACCAAGC AGATCAGGAG GACATAGAAG TAGAGGAAAG AAGTAATAAA 
CAACCCACAG AACAAACCAT TCACAGAGAA GTCAATGAGG ACAACGTGCA AACCTCAGGA 
GTGAGCACAC TCAGTTCAAC TTTGTCCCAC AACTCTCAGA ATGCCGACAG GTCCCTGAAT 
GTGCAACTTG AAAGACTTAG GTTAGAGTCA AACAGGCTTT TGGAAGAATT GTTACTTAAA 
GAGAGAGAAT TCCAAGTCCT TCTACGCCAA GCTATTGAAG TGAAGGACAG GGAAATCAAA 
CGCCTGCAGT GCACGTCTGA GCCAATAAGT GGGCCCTTGC TAACTGTGGA AGACCACAAT 
GATCCCACAT TCAAAACTCT CTCTTTTCAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819214.1
CDS	208..4140
Translation	MQVMSDSSEGISFSVPCSRLPGSSCCAHEDTGGLTLQENATICGGTRIPEAGSFWREDYLRASAVGSGKDAPCYGKWVNPPPTASGGISLRRITVVYVINEVSPELHACEAAALYSLQEACENHGALLETLDFGKLDFGETSVLDTFYNADIAVVEMNDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIIGQKNTMCTGNYTFIPYMITPQNKVFCCESIFMRGMTELMPSNFELLLGPICLPLVDRFVQILKVAQATSCQYFREAILNDIRRARNQYTGKDLAAELIRIRQRVDSIELLSPDIIINLLLSYRDIQDYDSVVTLVEILEKLPTFNLSSNHHIKFHYAFALNRRNHPGDRVKALEIMLPLVQLEDQVASDIYCIVGRIYKDMFLDSNFMDTESRDNAIHWYRKAFESEPTLHSGINYAVLLLAAGHQFETSLELRKIGVKLSSLLGKKGNLEKLQSYWDVGFYLGACILANDSSKVIHAAEKLFKLKTPAWFLNSVVETILIYQHFKTPNQEQHVTNHELVDFWMDFLVESTSSNVSVLRFPVLILEPTKIYQPSYLSINSEAEVQTISIWHVLPGDKKGIHEWNFPADSIRGVSISKFEERCCFLYVLNNSDDFQIYFCTELHCKKFFDKVNRIKEENGKNTEEGECDGDLMEFDYEYDENGDRVVLGKGKHGVVYAGRDLSNQVRIAIKEVPERDGRCSQPLHEEIALHKRLKHKNIVQYLGSFSKGGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPNTETFTGTLQYMAPEIIDKGPRGYGKPADIWSLGCTVIEMATGKPPFYELGEPQAAMFKVGMFKIHPDIPESMSSEAKAFLLRCFEPDPVKRATATELITDEFLKVSSRKRKSQLKLTTLSSGSNEYLRSISLPVSVMVEDTSSSSEYSSVSPDSDLRSDPFTFKTRARSCIEKDVKLNRSLFLGIPDENFEDSSAPPSPEERDSGFFMLKKDSERRATLYHILTEDQDKVARNLMDALVQGSEIKLKWDHITAIVDSLKEFVRSTDRKIISTTLSKLKLELDFDSHAISQVQMVLFGFQDAVNKVLRNHNIKPHWMFALDNIIRKAVQTAITILVPELRPHFSITSESDQADQEDIEVEERSNKQPTEQTIHREVNEDNVQTSGVSTLSSTLSHNSQNADRSLNVQLERLRLESNRLLEELLLKEREFQVLLRQAIEVKDREIKRLQCTSEPISGPLLTVEDHNDPTFKTLSFQ

Target ORF information:

RefSeq Version	XM_012963760.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963760.2

ORF Insert Sequence:

ATGCAAGTAA TGAGTGACAG CAGCGAAGGA ATCAGTTTCT CTGTACCATG CTCACGGCTG 
CCTGGCTCAA GCTGCTGTGC TCATGAAGAC ACAGGAGGGC TTACGCTACA GGAAAATGCC 
ACCATATGCG GTGGAACCAG GATCCCTGAA GCTGGAAGCT TCTGGCGTGA AGATTACCTC 
CGTGCTTCAG CGGTTGGAAG TGGTAAAGAT GCCCCATGCT ATGGAAAGTG GGTTAACCCC 
CCTCCTACAG CTAGCGGAGG TATTAGCCTT CGTAGGATTA CTGTGGTATA TGTTATCAAT 
GAAGTAAGTC CGGAGTTGCA TGCTTGTGAG GCTGCTGCAT TATACAGTTT GCAGGAGGCA 
TGTGAGAACC ATGGGGCACT GCTTGAGACC CTGGATTTCG GCAAACTGGA CTTTGGCGAG 
ACGTCAGTGC TAGACACCTT CTACAATGCA GATATTGCAG TGGTTGAGAT GAATGACGCT 
TTTCGACAGC CTTCTTTGTT CTACCATCTG GGAGTAAGAG AAAGCTTTAG CATGGCAAAC 
AACATAATTC TTTACTGTGA CACAAATTCA GACTCCCTGC AGTCACTGAA GGAAATTATC 
GGGCAAAAGA ATACTATGTG CACAGGTAAT TACACATTTA TTCCTTACAT GATCACGCCA 
CAAAACAAAG TGTTTTGCTG TGAGAGTATT TTCATGAGAG GAATGACTGA ACTAATGCCG 
TCAAACTTTG AGTTACTTCT TGGTCCGATC TGCCTGCCCC TAGTGGATCG CTTTGTTCAG 
ATTCTGAAGG TGGCACAGGC CACGTCATGC CAATATTTTC GAGAGGCAAT ACTAAATGAT 
ATTAGAAGGG CTCGGAACCA ATACACTGGG AAGGATCTGG CTGCTGAGCT AATAAGGATC 
CGCCAGCGCG TAGACAGCAT CGAGTTGTTA TCACCTGACA TCATTATCAA TCTGCTTTTG 
TCATACAGAG ATATCCAGGA CTACGATTCA GTTGTGACGT TGGTGGAAAT ATTGGAGAAA 
CTTCCAACCT TTAATTTATC TTCCAATCAC CATATAAAAT TTCATTATGC ATTTGCATTA 
AACAGGAGAA ATCATCCAGG TGACAGAGTA AAAGCCCTTG AAATAATGTT GCCTTTGGTG 
CAACTTGAAG ATCAAGTAGC TTCAGACATA TACTGCATTG TGGGAAGGAT CTACAAAGAT 
ATGTTCTTAG ATTCTAACTT CATGGATACC GAAAGTCGTG ATAATGCTAT ACATTGGTAC 
AGGAAGGCCT TTGAATCTGA ACCAACACTT CATTCTGGAA TCAATTATGC GGTGCTACTT 
CTAGCTGCTG GTCATCAATT TGAGACTTCT TTGGAACTCC GGAAAATCGG AGTTAAACTC 
AGTAGCCTGT TGGGTAAAAA AGGAAATCTC GAAAAACTCC AAAGTTATTG GGATGTTGGA 
TTTTATTTGG GGGCATGTAT ACTGGCCAAT GACTCATCAA AAGTTATCCA TGCAGCAGAA 
AAGCTTTTCA AGCTCAAAAC CCCAGCATGG TTCTTGAATT CAGTTGTGGA GACCATTTTG 
ATTTATCAGC ATTTTAAAAC GCCAAACCAA GAACAACATG TAACAAACCA TGAGCTGGTT 
GATTTTTGGA TGGATTTTCT TGTTGAGTCT ACAAGTAGCA ATGTGTCTGT ATTACGGTTT 
CCTGTCTTAA TACTAGAGCC AACAAAAATC TACCAGCCTT CCTACCTATC AATCAATAGT 
GAAGCTGAAG TTCAGACAAT ATCTATTTGG CACGTTCTAC CTGGCGATAA GAAAGGAATC 
CATGAATGGA ATTTCCCTGC AGATTCTATC AGAGGTGTAA GCATTTCAAA GTTTGAAGAA 
AGGTGCTGTT TTCTGTATGT TCTGAACAAC TCTGACGATT TTCAAATATA TTTCTGCACA 
GAGCTCCACT GCAAAAAATT CTTTGATAAA GTAAACCGTA TCAAAGAAGA GAATGGAAAG 
AACACAGAAG AAGGGGAGTG TGATGGAGAT TTAATGGAGT TCGACTATGA GTATGATGAA 
AATGGGGACA GAGTGGTTCT CGGTAAAGGG AAACATGGAG TGGTATATGC AGGAAGAGAC 
TTGAGTAACC AGGTCAGAAT TGCAATTAAA GAAGTTCCAG AGAGAGATGG TCGATGCTCT 
CAACCACTTC ATGAAGAGAT TGCTTTGCAT AAACGTCTAA AGCATAAAAA CATTGTTCAG 
TATTTGGGAT CATTCAGTAA AGGTGGTTTC ATTAAAATAT TTATGGAACA AGTACCTGGA 
GGAAGCCTCT CCGCACTTCT AAGGTCAAAA TGGGGACCCT TGAAAGACAA TGAGCAGACA 
ATCGGATTTT ATACCAAACA GATCCTTGAA GGTTTGAAAT ATCTCCATGA TAACCAGATA 
GTTCATAGAG ATATCAAGGG TGATAATGTT CTTATAAACA CATACAGTGG TGTTCTGAAA 
ATCTCTGATT TTGGCACTTC AAAGAGACTT GCTGGAATCA ACCCAAATAC AGAAACCTTC 
ACTGGCACTC TACAATATAT GGCACCAGAA ATTATAGACA AAGGACCAAG AGGATATGGA 
AAGCCAGCAG ACATTTGGTC TCTGGGATGC ACAGTTATAG AAATGGCAAC AGGAAAACCA 
CCATTTTATG AGCTTGGAGA ACCACAAGCT GCCATGTTTA AAGTGGGCAT GTTTAAAATT 
CATCCAGATA TCCCAGAATC TATGTCGTCT GAAGCTAAGG CATTTTTGTT GCGGTGCTTT 
GAACCAGATC CTGTGAAACG CGCTACTGCT ACTGAACTTA TAACGGATGA ATTCTTGAAG 
GTGTCCTCTA GGAAACGCAA GTCCCAGCTT AAGCTTACAA CTCTTTCCTC TGGCTCAAAT 
GAATATCTCC GTAGCATATC TTTGCCGGTG TCAGTGATGG TGGAAGATAC AAGCAGCAGC 
AGTGAATACA GTTCCGTTTC TCCAGATTCA GACTTAAGAT CGGACCCATT TACTTTTAAA 
ACAAGAGCCA GATCATGTAT AGAAAAGGAT GTCAAGCTTA ACAGAAGTTT ATTTCTAGGA 
ATTCCAGACG AGAATTTTGA AGACAGTAGT GCTCCTCCTT CTCCTGAAGA ACGAGACTCT 
GGATTTTTCA TGCTGAAGAA AGATAGTGAA AGGAGGGCTA CACTTTATCA TATTCTTACA 
GAGGATCAGG ACAAGGTGGC TAGAAACTTA ATGGATGCTT TAGTCCAGGG ATCAGAAATA 
AAATTGAAAT GGGACCACAT CACTGCTATT GTTGACAGCC TAAAAGAGTT TGTAAGATCT 
ACTGACAGGA AAATCATTTC AACAACACTA TCAAAACTCA AACTTGAGTT AGACTTTGAT 
AGTCATGCTA TCAGTCAAGT CCAGATGGTG CTGTTTGGCT TTCAAGATGC GGTAAATAAA 
GTGCTACGAA ATCATAACAT AAAGCCTCAC TGGATGTTTG CCTTAGATAA TATAATTCGA 
AAGGCCGTGC AGACAGCTAT CACTATCCTG GTACCTGAGC TGAGGCCACA TTTTAGCATT 
ACATCAGAAA GTGACCAAGC AGATCAGGAG GACATAGAAG TAGAGGAAAG AAGTAATAAA 
CAACCCACAG AACAAACCAT TCACAGAGAA GTCAATGAGG ACAACGTGCA AACCTCAGGA 
GTGAGCACAC TCAGTTCAAC TTTGTCCCAC AACTCTCAGA ATGCCGACAG GTCCCTGAAT 
GTGCAACTTG AAAGACTTAG GTTAGAGTCA AACAGGCTTT TGGAAGAATT GTTACTTAAA 
GAGAGAGAAT TCCAAGTCCT TCTACGCCAA GCTATTGAAG TGAAGGACAG GGAAATCAAA 
CGCCTGCAGT GCACGTCTGA GCCAATAAGT GGGCCCTTGC TAACTGTGGA AGACCACAAT 
GATCCCACAT TCAAAACTCT CTCTTTTCAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k5 ( XM_012963761.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819215.1 Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X6, mRNA.

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CloneID	OXa36967
Clone ID Related Accession (Same CDS sequence)	XM_012963761.2 , XM_018094280.1
Accession Version	XM_012963761.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963761.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTAA TGAGTGACAG CAGCGAAGGA ATCAGTTTCT CTGTACCATG CTCACGGCTG 
CCTGGCTCAA GCTGCTGTGC TCATGAAGAC ACAGGAGGGC TTACGCTACA GGAAAATGCC 
ACCATATGCG GTGGAACCAG GATCCCTGAA GCTGGAAGCT TCTGGCGTGA AGATTACCTC 
CGTGCTTCAG CGGTTGGAAG TGGTAAAGAT GCCCCATGCT ATGGAAAGTG GGTTAACCCC 
CCTCCTACAG CTAGCGGAGG TATTAGCCTT CGTAGGATTA CTGTGGTATA TGTTATCAAT 
GAAGTAAGTC CGGAGTTGCA TGCTTGTGAG GCTGCTGCAT TATACAGTTT GCAGGAGGCA 
TGTGAGAACC ATGGGGCACT GCTTGAGACC CTGGATTTCG GCAAACTGGA CTTTGGCGAG 
ACGTCAGTGC TAGACACCTT CTACAATGCA GATATTGCAG TGGTTGAGAT GAATGACGCT 
TTTCGACAGC CTTCTTTGTT CTACCATCTG GGAGTAAGAG AAAGCTTTAG CATGGCAAAC 
AACATAATTC TTTACTGTGA CACAAATTCA GACTCCCTGC AGTCACTGAA GGAAATTATC 
GGGCAAAAGA ATACTATGTG CACAGGTAAT TACACATTTA TTCCTTACAT GATCACGCCA 
CAAAACAAAG TGTTTTGCTG TGAGAGTATT TTCATGAGAG GAATGACTGA ACTAATGCCG 
TCAAACTTTG AGTTACTTCT TGGTCCGATC TGCCTGCCCC TAGTGGATCG CTTTGTTCAG 
ATTCTGAAGG TGGCACAGGC CACGTCATGC CAATATTTTC GAGAGGCAAT ACTAAATGAT 
ATTAGAAGGG CTCGGAACCA ATACACTGGG AAGGATCTGG CTGCTGAGCT AATAAGGATC 
CGCCAGCGCG TAGACAGCAT CGAGTTGTTA TCACCTGACA TCATTATCAA TCTGCTTTTG 
TCATACAGAG ATATCCAGGA CTACGATTCA GTTGTGACGT TGGTGGAAAT ATTGGAGAAA 
CTTCCAACCT TTAATTTATC TTCCAATCAC CATATAAAAT TTCATTATGC ATTTGCATTA 
AACAGGAGAA ATCATCCAGG TGACAGAGTA AAAGCCCTTG AAATAATGTT GCCTTTGGTG 
CAACTTGAAG ATCAAGTAGC TTCAGACATA TACTGCATTG TGGGAAGGAT CTACAAAGAT 
ATGTTCTTAG ATTCTAACTT CATGGATACC GAAAGTCGTG ATAATGCTAT ACATTGGTAC 
AGGAAGGCCT TTGAATCTGA ACCAACACTT CATTCTGGAA TCAATTATGC GGTGCTACTT 
CTAGCTGCTG GTCATCAATT TGAGACTTCT TTGGAACTCC GGAAAATCGG AGTTAAACTC 
AGTAGCCTGT TGGGTAAAAA AGGAAATCTC GAAAAACTCC AAAGTTATTG GGATGTTGGA 
TTTTATTTGG GGGCATGTAT ACTGGCCAAT GACTCATCAA AAGTTATCCA TGCAGCAGAA 
AAGCTTTTCA AGCTCAAAAC CCCAGCATGG TTCTTGAATT CAGTTGTGGA GACCATTTTG 
ATTTATCAGC ATTTTAAAAC GCCAAACCAA GAACAACATG TAACAAACCA TGAGCTGGTT 
GATTTTTGGA TGGATTTTCT TGTTGAGTCT ACAAGTAGCA ATGTGTCTGT ATTACGGTTT 
CCTGTCTTAA TACTAGAGCC AACAAAAATC TACCAGCCTT CCTACCTATC AATCAATAGT 
GAAGCTGAAG TTCAGACAAT ATCTATTTGG CACGTTCTAC CTGGCGATAA GAAAGGAATC 
CATGAATGGA ATTTCCCTGC AGATTCTATC AGAGGTGTAA GCATTTCAAA GTTTGAAGAA 
AGGTGCTGTT TTCTGTATGT TCTGAACAAC TCTGACGATT TTCAAATATA TTTCTGCACA 
GAGCTCCACT GCAAAAAATT CTTTGATAAA GTAAACCGTA TCAAAGAAGA GAATGGAAAG 
AACACAGAAG AAGGGGAGTG TGATGGAGAT TTAATGGAGT TCGACTATGA GTATGATGAA 
AATGGGGACA GAGTGGTTCT CGGTAAAGGG AAACATGGAG TGGTATATGC AGGAAGAGAC 
TTGAGTAACC AGGTCAGAAT TGCAATTAAA GAAGTTCCAG AGAGAGATGG TCGATGCTCT 
CAACCACTTC ATGAAGAGAT TGCTTTGCAT AAACGTCTAA AGCATAAAAA CATTGTTCAG 
TATTTGGGAT CATTCAGTAA AGGTGGTTTC ATTAAAATAT TTATGGAACA AGTACCTGGA 
GGAAGCCTCT CCGCACTTCT AAGGTCAAAA TGGGGACCCT TGAAAGACAA TGAGCAGACA 
ATCGGATTTT ATACCAAACA GATCCTTGAA GGTTTGAAAT ATCTCCATGA TAACCAGATA 
GTTCATAGAG ATATCAAGGG TGATAATGTT CTTATAAACA CATACAGTGG TGTTCTGAAA 
ATCTCTGATT TTGGCACTTC AAAGAGACTT GCTGGAATCA ACCCAAATAC AGAAACCTTC 
ACTGGCACTC TACAATATAT GGCACCAGAA ATTATAGACA AAGGACCAAG AGGATATGGA 
AAGCCAGCAG ACATTTGGTC TCTGGGATGC ACAGTTATAG AAATGGCAAC AGGAAAACCA 
CCATTTTATG AGCTTGGAGA ACCACAAGCT GCCATGTTTA AAGTGGGCAT GTTTAAAATT 
CATCCAGATA TCCCAGAATC TATGTCGTCT GAAGCTAAGG CATTTTTGTT GCGGTGCTTT 
GAACCAGATC CTGTGAAACG CGCTACTGCT ACTGAACTTA TAACGGATGA ATTCTTGAAG 
GTGTCCTCTA GGAAACGCAA GTCCCAGCTT AAGCTTACAA CTCTTTCCTC TGGCTCAAAT 
GAATATCTCC GTAGCATATC TTTGCCGGTG TCAGTGATGG TGGAAGATAC AAGCAGCAGC 
AGTGAATACA GTTCCGTTTC TCCAGATTCA GACTTAAGAT CGGACCCATT TACTTTTAAA 
ACAAGAGCCA GATCATGTAT AGAAAAGGAT GTCAAGCTTA ACAGAAGTTT ATTTCTAGGA 
ATTCCAGACG AGAATTTTGA AGACAGTAGT GCTCCTCCTT CTCCTGAAGA ACGAGACTCT 
GGATTTTTCA TGCTGAAGAA AGATAGTGAA AGGAGGGCTA CACTTTATCA TATTCTTACA 
GAGGATCAGG ACAAGGTGGC TAGAAACTTA ATGGATGCTT TAGTCCAGGG ATCAGAAATA 
AAATTGAAAT GGGACCACAT CACTGCTATT GTTGACAGCC TAAAAGAGTT TGTAAGATCT 
ACTGACAGGA AAATCATTTC AACAACACTA TCAAAACTCA AACTTGAGTT AGACTTTGAT 
AGTCATGCTA TCAGTCAAGT CCAGATGGTG CTGTTTGGCT TTCAAGATGC GGTAAATAAA 
GTGCTACGAA ATCATAACAT AAAGCCTCAC TGGATGTTTG CCTTAGATAA TATAATTCGA 
AAGGCCGTGC AGACAGCTAT CACTATCCTG GTACCTGGCT TTTGGAAGAA TTGTTACTTA 
AAGAGAGAGA ATTCCAAGTC CTTCTACGCC AAGCTATTGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819215.1
CDS	207..3791
Translation	MQVMSDSSEGISFSVPCSRLPGSSCCAHEDTGGLTLQENATICGGTRIPEAGSFWREDYLRASAVGSGKDAPCYGKWVNPPPTASGGISLRRITVVYVINEVSPELHACEAAALYSLQEACENHGALLETLDFGKLDFGETSVLDTFYNADIAVVEMNDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIIGQKNTMCTGNYTFIPYMITPQNKVFCCESIFMRGMTELMPSNFELLLGPICLPLVDRFVQILKVAQATSCQYFREAILNDIRRARNQYTGKDLAAELIRIRQRVDSIELLSPDIIINLLLSYRDIQDYDSVVTLVEILEKLPTFNLSSNHHIKFHYAFALNRRNHPGDRVKALEIMLPLVQLEDQVASDIYCIVGRIYKDMFLDSNFMDTESRDNAIHWYRKAFESEPTLHSGINYAVLLLAAGHQFETSLELRKIGVKLSSLLGKKGNLEKLQSYWDVGFYLGACILANDSSKVIHAAEKLFKLKTPAWFLNSVVETILIYQHFKTPNQEQHVTNHELVDFWMDFLVESTSSNVSVLRFPVLILEPTKIYQPSYLSINSEAEVQTISIWHVLPGDKKGIHEWNFPADSIRGVSISKFEERCCFLYVLNNSDDFQIYFCTELHCKKFFDKVNRIKEENGKNTEEGECDGDLMEFDYEYDENGDRVVLGKGKHGVVYAGRDLSNQVRIAIKEVPERDGRCSQPLHEEIALHKRLKHKNIVQYLGSFSKGGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPNTETFTGTLQYMAPEIIDKGPRGYGKPADIWSLGCTVIEMATGKPPFYELGEPQAAMFKVGMFKIHPDIPESMSSEAKAFLLRCFEPDPVKRATATELITDEFLKVSSRKRKSQLKLTTLSSGSNEYLRSISLPVSVMVEDTSSSSEYSSVSPDSDLRSDPFTFKTRARSCIEKDVKLNRSLFLGIPDENFEDSSAPPSPEERDSGFFMLKKDSERRATLYHILTEDQDKVARNLMDALVQGSEIKLKWDHITAIVDSLKEFVRSTDRKIISTTLSKLKLELDFDSHAISQVQMVLFGFQDAVNKVLRNHNIKPHWMFALDNIIRKAVQTAITILVPGFWKNCYLKRENSKSFYAKLLK

Target ORF information:

RefSeq Version	XM_012963761.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963761.2

ORF Insert Sequence:

ATGCAAGTAA TGAGTGACAG CAGCGAAGGA ATCAGTTTCT CTGTACCATG CTCACGGCTG 
CCTGGCTCAA GCTGCTGTGC TCATGAAGAC ACAGGAGGGC TTACGCTACA GGAAAATGCC 
ACCATATGCG GTGGAACCAG GATCCCTGAA GCTGGAAGCT TCTGGCGTGA AGATTACCTC 
CGTGCTTCAG CGGTTGGAAG TGGTAAAGAT GCCCCATGCT ATGGAAAGTG GGTTAACCCC 
CCTCCTACAG CTAGCGGAGG TATTAGCCTT CGTAGGATTA CTGTGGTATA TGTTATCAAT 
GAAGTAAGTC CGGAGTTGCA TGCTTGTGAG GCTGCTGCAT TATACAGTTT GCAGGAGGCA 
TGTGAGAACC ATGGGGCACT GCTTGAGACC CTGGATTTCG GCAAACTGGA CTTTGGCGAG 
ACGTCAGTGC TAGACACCTT CTACAATGCA GATATTGCAG TGGTTGAGAT GAATGACGCT 
TTTCGACAGC CTTCTTTGTT CTACCATCTG GGAGTAAGAG AAAGCTTTAG CATGGCAAAC 
AACATAATTC TTTACTGTGA CACAAATTCA GACTCCCTGC AGTCACTGAA GGAAATTATC 
GGGCAAAAGA ATACTATGTG CACAGGTAAT TACACATTTA TTCCTTACAT GATCACGCCA 
CAAAACAAAG TGTTTTGCTG TGAGAGTATT TTCATGAGAG GAATGACTGA ACTAATGCCG 
TCAAACTTTG AGTTACTTCT TGGTCCGATC TGCCTGCCCC TAGTGGATCG CTTTGTTCAG 
ATTCTGAAGG TGGCACAGGC CACGTCATGC CAATATTTTC GAGAGGCAAT ACTAAATGAT 
ATTAGAAGGG CTCGGAACCA ATACACTGGG AAGGATCTGG CTGCTGAGCT AATAAGGATC 
CGCCAGCGCG TAGACAGCAT CGAGTTGTTA TCACCTGACA TCATTATCAA TCTGCTTTTG 
TCATACAGAG ATATCCAGGA CTACGATTCA GTTGTGACGT TGGTGGAAAT ATTGGAGAAA 
CTTCCAACCT TTAATTTATC TTCCAATCAC CATATAAAAT TTCATTATGC ATTTGCATTA 
AACAGGAGAA ATCATCCAGG TGACAGAGTA AAAGCCCTTG AAATAATGTT GCCTTTGGTG 
CAACTTGAAG ATCAAGTAGC TTCAGACATA TACTGCATTG TGGGAAGGAT CTACAAAGAT 
ATGTTCTTAG ATTCTAACTT CATGGATACC GAAAGTCGTG ATAATGCTAT ACATTGGTAC 
AGGAAGGCCT TTGAATCTGA ACCAACACTT CATTCTGGAA TCAATTATGC GGTGCTACTT 
CTAGCTGCTG GTCATCAATT TGAGACTTCT TTGGAACTCC GGAAAATCGG AGTTAAACTC 
AGTAGCCTGT TGGGTAAAAA AGGAAATCTC GAAAAACTCC AAAGTTATTG GGATGTTGGA 
TTTTATTTGG GGGCATGTAT ACTGGCCAAT GACTCATCAA AAGTTATCCA TGCAGCAGAA 
AAGCTTTTCA AGCTCAAAAC CCCAGCATGG TTCTTGAATT CAGTTGTGGA GACCATTTTG 
ATTTATCAGC ATTTTAAAAC GCCAAACCAA GAACAACATG TAACAAACCA TGAGCTGGTT 
GATTTTTGGA TGGATTTTCT TGTTGAGTCT ACAAGTAGCA ATGTGTCTGT ATTACGGTTT 
CCTGTCTTAA TACTAGAGCC AACAAAAATC TACCAGCCTT CCTACCTATC AATCAATAGT 
GAAGCTGAAG TTCAGACAAT ATCTATTTGG CACGTTCTAC CTGGCGATAA GAAAGGAATC 
CATGAATGGA ATTTCCCTGC AGATTCTATC AGAGGTGTAA GCATTTCAAA GTTTGAAGAA 
AGGTGCTGTT TTCTGTATGT TCTGAACAAC TCTGACGATT TTCAAATATA TTTCTGCACA 
GAGCTCCACT GCAAAAAATT CTTTGATAAA GTAAACCGTA TCAAAGAAGA GAATGGAAAG 
AACACAGAAG AAGGGGAGTG TGATGGAGAT TTAATGGAGT TCGACTATGA GTATGATGAA 
AATGGGGACA GAGTGGTTCT CGGTAAAGGG AAACATGGAG TGGTATATGC AGGAAGAGAC 
TTGAGTAACC AGGTCAGAAT TGCAATTAAA GAAGTTCCAG AGAGAGATGG TCGATGCTCT 
CAACCACTTC ATGAAGAGAT TGCTTTGCAT AAACGTCTAA AGCATAAAAA CATTGTTCAG 
TATTTGGGAT CATTCAGTAA AGGTGGTTTC ATTAAAATAT TTATGGAACA AGTACCTGGA 
GGAAGCCTCT CCGCACTTCT AAGGTCAAAA TGGGGACCCT TGAAAGACAA TGAGCAGACA 
ATCGGATTTT ATACCAAACA GATCCTTGAA GGTTTGAAAT ATCTCCATGA TAACCAGATA 
GTTCATAGAG ATATCAAGGG TGATAATGTT CTTATAAACA CATACAGTGG TGTTCTGAAA 
ATCTCTGATT TTGGCACTTC AAAGAGACTT GCTGGAATCA ACCCAAATAC AGAAACCTTC 
ACTGGCACTC TACAATATAT GGCACCAGAA ATTATAGACA AAGGACCAAG AGGATATGGA 
AAGCCAGCAG ACATTTGGTC TCTGGGATGC ACAGTTATAG AAATGGCAAC AGGAAAACCA 
CCATTTTATG AGCTTGGAGA ACCACAAGCT GCCATGTTTA AAGTGGGCAT GTTTAAAATT 
CATCCAGATA TCCCAGAATC TATGTCGTCT GAAGCTAAGG CATTTTTGTT GCGGTGCTTT 
GAACCAGATC CTGTGAAACG CGCTACTGCT ACTGAACTTA TAACGGATGA ATTCTTGAAG 
GTGTCCTCTA GGAAACGCAA GTCCCAGCTT AAGCTTACAA CTCTTTCCTC TGGCTCAAAT 
GAATATCTCC GTAGCATATC TTTGCCGGTG TCAGTGATGG TGGAAGATAC AAGCAGCAGC 
AGTGAATACA GTTCCGTTTC TCCAGATTCA GACTTAAGAT CGGACCCATT TACTTTTAAA 
ACAAGAGCCA GATCATGTAT AGAAAAGGAT GTCAAGCTTA ACAGAAGTTT ATTTCTAGGA 
ATTCCAGACG AGAATTTTGA AGACAGTAGT GCTCCTCCTT CTCCTGAAGA ACGAGACTCT 
GGATTTTTCA TGCTGAAGAA AGATAGTGAA AGGAGGGCTA CACTTTATCA TATTCTTACA 
GAGGATCAGG ACAAGGTGGC TAGAAACTTA ATGGATGCTT TAGTCCAGGG ATCAGAAATA 
AAATTGAAAT GGGACCACAT CACTGCTATT GTTGACAGCC TAAAAGAGTT TGTAAGATCT 
ACTGACAGGA AAATCATTTC AACAACACTA TCAAAACTCA AACTTGAGTT AGACTTTGAT 
AGTCATGCTA TCAGTCAAGT CCAGATGGTG CTGTTTGGCT TTCAAGATGC GGTAAATAAA 
GTGCTACGAA ATCATAACAT AAAGCCTCAC TGGATGTTTG CCTTAGATAA TATAATTCGA 
AAGGCCGTGC AGACAGCTAT CACTATCCTG GTACCTGGCT TTTGGAAGAA TTGTTACTTA 
AAGAGAGAGA ATTCCAAGTC CTTCTACGCC AAGCTATTGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k5 ( XM_018094280.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949769.1 Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X7, mRNA.

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CloneID	OXa36967
Clone ID Related Accession (Same CDS sequence)	XM_012963761.2 , XM_018094280.1
Accession Version	XM_018094280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTAA TGAGTGACAG CAGCGAAGGA ATCAGTTTCT CTGTACCATG CTCACGGCTG 
CCTGGCTCAA GCTGCTGTGC TCATGAAGAC ACAGGAGGGC TTACGCTACA GGAAAATGCC 
ACCATATGCG GTGGAACCAG GATCCCTGAA GCTGGAAGCT TCTGGCGTGA AGATTACCTC 
CGTGCTTCAG CGGTTGGAAG TGGTAAAGAT GCCCCATGCT ATGGAAAGTG GGTTAACCCC 
CCTCCTACAG CTAGCGGAGG TATTAGCCTT CGTAGGATTA CTGTGGTATA TGTTATCAAT 
GAAGTAAGTC CGGAGTTGCA TGCTTGTGAG GCTGCTGCAT TATACAGTTT GCAGGAGGCA 
TGTGAGAACC ATGGGGCACT GCTTGAGACC CTGGATTTCG GCAAACTGGA CTTTGGCGAG 
ACGTCAGTGC TAGACACCTT CTACAATGCA GATATTGCAG TGGTTGAGAT GAATGACGCT 
TTTCGACAGC CTTCTTTGTT CTACCATCTG GGAGTAAGAG AAAGCTTTAG CATGGCAAAC 
AACATAATTC TTTACTGTGA CACAAATTCA GACTCCCTGC AGTCACTGAA GGAAATTATC 
GGGCAAAAGA ATACTATGTG CACAGGTAAT TACACATTTA TTCCTTACAT GATCACGCCA 
CAAAACAAAG TGTTTTGCTG TGAGAGTATT TTCATGAGAG GAATGACTGA ACTAATGCCG 
TCAAACTTTG AGTTACTTCT TGGTCCGATC TGCCTGCCCC TAGTGGATCG CTTTGTTCAG 
ATTCTGAAGG TGGCACAGGC CACGTCATGC CAATATTTTC GAGAGGCAAT ACTAAATGAT 
ATTAGAAGGG CTCGGAACCA ATACACTGGG AAGGATCTGG CTGCTGAGCT AATAAGGATC 
CGCCAGCGCG TAGACAGCAT CGAGTTGTTA TCACCTGACA TCATTATCAA TCTGCTTTTG 
TCATACAGAG ATATCCAGGA CTACGATTCA GTTGTGACGT TGGTGGAAAT ATTGGAGAAA 
CTTCCAACCT TTAATTTATC TTCCAATCAC CATATAAAAT TTCATTATGC ATTTGCATTA 
AACAGGAGAA ATCATCCAGG TGACAGAGTA AAAGCCCTTG AAATAATGTT GCCTTTGGTG 
CAACTTGAAG ATCAAGTAGC TTCAGACATA TACTGCATTG TGGGAAGGAT CTACAAAGAT 
ATGTTCTTAG ATTCTAACTT CATGGATACC GAAAGTCGTG ATAATGCTAT ACATTGGTAC 
AGGAAGGCCT TTGAATCTGA ACCAACACTT CATTCTGGAA TCAATTATGC GGTGCTACTT 
CTAGCTGCTG GTCATCAATT TGAGACTTCT TTGGAACTCC GGAAAATCGG AGTTAAACTC 
AGTAGCCTGT TGGGTAAAAA AGGAAATCTC GAAAAACTCC AAAGTTATTG GGATGTTGGA 
TTTTATTTGG GGGCATGTAT ACTGGCCAAT GACTCATCAA AAGTTATCCA TGCAGCAGAA 
AAGCTTTTCA AGCTCAAAAC CCCAGCATGG TTCTTGAATT CAGTTGTGGA GACCATTTTG 
ATTTATCAGC ATTTTAAAAC GCCAAACCAA GAACAACATG TAACAAACCA TGAGCTGGTT 
GATTTTTGGA TGGATTTTCT TGTTGAGTCT ACAAGTAGCA ATGTGTCTGT ATTACGGTTT 
CCTGTCTTAA TACTAGAGCC AACAAAAATC TACCAGCCTT CCTACCTATC AATCAATAGT 
GAAGCTGAAG TTCAGACAAT ATCTATTTGG CACGTTCTAC CTGGCGATAA GAAAGGAATC 
CATGAATGGA ATTTCCCTGC AGATTCTATC AGAGGTGTAA GCATTTCAAA GTTTGAAGAA 
AGGTGCTGTT TTCTGTATGT TCTGAACAAC TCTGACGATT TTCAAATATA TTTCTGCACA 
GAGCTCCACT GCAAAAAATT CTTTGATAAA GTAAACCGTA TCAAAGAAGA GAATGGAAAG 
AACACAGAAG AAGGGGAGTG TGATGGAGAT TTAATGGAGT TCGACTATGA GTATGATGAA 
AATGGGGACA GAGTGGTTCT CGGTAAAGGG AAACATGGAG TGGTATATGC AGGAAGAGAC 
TTGAGTAACC AGGTCAGAAT TGCAATTAAA GAAGTTCCAG AGAGAGATGG TCGATGCTCT 
CAACCACTTC ATGAAGAGAT TGCTTTGCAT AAACGTCTAA AGCATAAAAA CATTGTTCAG 
TATTTGGGAT CATTCAGTAA AGGTGGTTTC ATTAAAATAT TTATGGAACA AGTACCTGGA 
GGAAGCCTCT CCGCACTTCT AAGGTCAAAA TGGGGACCCT TGAAAGACAA TGAGCAGACA 
ATCGGATTTT ATACCAAACA GATCCTTGAA GGTTTGAAAT ATCTCCATGA TAACCAGATA 
GTTCATAGAG ATATCAAGGG TGATAATGTT CTTATAAACA CATACAGTGG TGTTCTGAAA 
ATCTCTGATT TTGGCACTTC AAAGAGACTT GCTGGAATCA ACCCAAATAC AGAAACCTTC 
ACTGGCACTC TACAATATAT GGCACCAGAA ATTATAGACA AAGGACCAAG AGGATATGGA 
AAGCCAGCAG ACATTTGGTC TCTGGGATGC ACAGTTATAG AAATGGCAAC AGGAAAACCA 
CCATTTTATG AGCTTGGAGA ACCACAAGCT GCCATGTTTA AAGTGGGCAT GTTTAAAATT 
CATCCAGATA TCCCAGAATC TATGTCGTCT GAAGCTAAGG CATTTTTGTT GCGGTGCTTT 
GAACCAGATC CTGTGAAACG CGCTACTGCT ACTGAACTTA TAACGGATGA ATTCTTGAAG 
GTGTCCTCTA GGAAACGCAA GTCCCAGCTT AAGCTTACAA CTCTTTCCTC TGGCTCAAAT 
GAATATCTCC GTAGCATATC TTTGCCGGTG TCAGTGATGG TGGAAGATAC AAGCAGCAGC 
AGTGAATACA GTTCCGTTTC TCCAGATTCA GACTTAAGAT CGGACCCATT TACTTTTAAA 
ACAAGAGCCA GATCATGTAT AGAAAAGGAT GTCAAGCTTA ACAGAAGTTT ATTTCTAGGA 
ATTCCAGACG AGAATTTTGA AGACAGTAGT GCTCCTCCTT CTCCTGAAGA ACGAGACTCT 
GGATTTTTCA TGCTGAAGAA AGATAGTGAA AGGAGGGCTA CACTTTATCA TATTCTTACA 
GAGGATCAGG ACAAGGTGGC TAGAAACTTA ATGGATGCTT TAGTCCAGGG ATCAGAAATA 
AAATTGAAAT GGGACCACAT CACTGCTATT GTTGACAGCC TAAAAGAGTT TGTAAGATCT 
ACTGACAGGA AAATCATTTC AACAACACTA TCAAAACTCA AACTTGAGTT AGACTTTGAT 
AGTCATGCTA TCAGTCAAGT CCAGATGGTG CTGTTTGGCT TTCAAGATGC GGTAAATAAA 
GTGCTACGAA ATCATAACAT AAAGCCTCAC TGGATGTTTG CCTTAGATAA TATAATTCGA 
AAGGCCGTGC AGACAGCTAT CACTATCCTG GTACCTGGCT TTTGGAAGAA TTGTTACTTA 
AAGAGAGAGA ATTCCAAGTC CTTCTACGCC AAGCTATTGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949769.1
CDS	208..3792
Translation	MQVMSDSSEGISFSVPCSRLPGSSCCAHEDTGGLTLQENATICGGTRIPEAGSFWREDYLRASAVGSGKDAPCYGKWVNPPPTASGGISLRRITVVYVINEVSPELHACEAAALYSLQEACENHGALLETLDFGKLDFGETSVLDTFYNADIAVVEMNDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIIGQKNTMCTGNYTFIPYMITPQNKVFCCESIFMRGMTELMPSNFELLLGPICLPLVDRFVQILKVAQATSCQYFREAILNDIRRARNQYTGKDLAAELIRIRQRVDSIELLSPDIIINLLLSYRDIQDYDSVVTLVEILEKLPTFNLSSNHHIKFHYAFALNRRNHPGDRVKALEIMLPLVQLEDQVASDIYCIVGRIYKDMFLDSNFMDTESRDNAIHWYRKAFESEPTLHSGINYAVLLLAAGHQFETSLELRKIGVKLSSLLGKKGNLEKLQSYWDVGFYLGACILANDSSKVIHAAEKLFKLKTPAWFLNSVVETILIYQHFKTPNQEQHVTNHELVDFWMDFLVESTSSNVSVLRFPVLILEPTKIYQPSYLSINSEAEVQTISIWHVLPGDKKGIHEWNFPADSIRGVSISKFEERCCFLYVLNNSDDFQIYFCTELHCKKFFDKVNRIKEENGKNTEEGECDGDLMEFDYEYDENGDRVVLGKGKHGVVYAGRDLSNQVRIAIKEVPERDGRCSQPLHEEIALHKRLKHKNIVQYLGSFSKGGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPNTETFTGTLQYMAPEIIDKGPRGYGKPADIWSLGCTVIEMATGKPPFYELGEPQAAMFKVGMFKIHPDIPESMSSEAKAFLLRCFEPDPVKRATATELITDEFLKVSSRKRKSQLKLTTLSSGSNEYLRSISLPVSVMVEDTSSSSEYSSVSPDSDLRSDPFTFKTRARSCIEKDVKLNRSLFLGIPDENFEDSSAPPSPEERDSGFFMLKKDSERRATLYHILTEDQDKVARNLMDALVQGSEIKLKWDHITAIVDSLKEFVRSTDRKIISTTLSKLKLELDFDSHAISQVQMVLFGFQDAVNKVLRNHNIKPHWMFALDNIIRKAVQTAITILVPGFWKNCYLKRENSKSFYAKLLK

Target ORF information:

RefSeq Version	XM_018094280.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094280.1

ORF Insert Sequence:

ATGCAAGTAA TGAGTGACAG CAGCGAAGGA ATCAGTTTCT CTGTACCATG CTCACGGCTG 
CCTGGCTCAA GCTGCTGTGC TCATGAAGAC ACAGGAGGGC TTACGCTACA GGAAAATGCC 
ACCATATGCG GTGGAACCAG GATCCCTGAA GCTGGAAGCT TCTGGCGTGA AGATTACCTC 
CGTGCTTCAG CGGTTGGAAG TGGTAAAGAT GCCCCATGCT ATGGAAAGTG GGTTAACCCC 
CCTCCTACAG CTAGCGGAGG TATTAGCCTT CGTAGGATTA CTGTGGTATA TGTTATCAAT 
GAAGTAAGTC CGGAGTTGCA TGCTTGTGAG GCTGCTGCAT TATACAGTTT GCAGGAGGCA 
TGTGAGAACC ATGGGGCACT GCTTGAGACC CTGGATTTCG GCAAACTGGA CTTTGGCGAG 
ACGTCAGTGC TAGACACCTT CTACAATGCA GATATTGCAG TGGTTGAGAT GAATGACGCT 
TTTCGACAGC CTTCTTTGTT CTACCATCTG GGAGTAAGAG AAAGCTTTAG CATGGCAAAC 
AACATAATTC TTTACTGTGA CACAAATTCA GACTCCCTGC AGTCACTGAA GGAAATTATC 
GGGCAAAAGA ATACTATGTG CACAGGTAAT TACACATTTA TTCCTTACAT GATCACGCCA 
CAAAACAAAG TGTTTTGCTG TGAGAGTATT TTCATGAGAG GAATGACTGA ACTAATGCCG 
TCAAACTTTG AGTTACTTCT TGGTCCGATC TGCCTGCCCC TAGTGGATCG CTTTGTTCAG 
ATTCTGAAGG TGGCACAGGC CACGTCATGC CAATATTTTC GAGAGGCAAT ACTAAATGAT 
ATTAGAAGGG CTCGGAACCA ATACACTGGG AAGGATCTGG CTGCTGAGCT AATAAGGATC 
CGCCAGCGCG TAGACAGCAT CGAGTTGTTA TCACCTGACA TCATTATCAA TCTGCTTTTG 
TCATACAGAG ATATCCAGGA CTACGATTCA GTTGTGACGT TGGTGGAAAT ATTGGAGAAA 
CTTCCAACCT TTAATTTATC TTCCAATCAC CATATAAAAT TTCATTATGC ATTTGCATTA 
AACAGGAGAA ATCATCCAGG TGACAGAGTA AAAGCCCTTG AAATAATGTT GCCTTTGGTG 
CAACTTGAAG ATCAAGTAGC TTCAGACATA TACTGCATTG TGGGAAGGAT CTACAAAGAT 
ATGTTCTTAG ATTCTAACTT CATGGATACC GAAAGTCGTG ATAATGCTAT ACATTGGTAC 
AGGAAGGCCT TTGAATCTGA ACCAACACTT CATTCTGGAA TCAATTATGC GGTGCTACTT 
CTAGCTGCTG GTCATCAATT TGAGACTTCT TTGGAACTCC GGAAAATCGG AGTTAAACTC 
AGTAGCCTGT TGGGTAAAAA AGGAAATCTC GAAAAACTCC AAAGTTATTG GGATGTTGGA 
TTTTATTTGG GGGCATGTAT ACTGGCCAAT GACTCATCAA AAGTTATCCA TGCAGCAGAA 
AAGCTTTTCA AGCTCAAAAC CCCAGCATGG TTCTTGAATT CAGTTGTGGA GACCATTTTG 
ATTTATCAGC ATTTTAAAAC GCCAAACCAA GAACAACATG TAACAAACCA TGAGCTGGTT 
GATTTTTGGA TGGATTTTCT TGTTGAGTCT ACAAGTAGCA ATGTGTCTGT ATTACGGTTT 
CCTGTCTTAA TACTAGAGCC AACAAAAATC TACCAGCCTT CCTACCTATC AATCAATAGT 
GAAGCTGAAG TTCAGACAAT ATCTATTTGG CACGTTCTAC CTGGCGATAA GAAAGGAATC 
CATGAATGGA ATTTCCCTGC AGATTCTATC AGAGGTGTAA GCATTTCAAA GTTTGAAGAA 
AGGTGCTGTT TTCTGTATGT TCTGAACAAC TCTGACGATT TTCAAATATA TTTCTGCACA 
GAGCTCCACT GCAAAAAATT CTTTGATAAA GTAAACCGTA TCAAAGAAGA GAATGGAAAG 
AACACAGAAG AAGGGGAGTG TGATGGAGAT TTAATGGAGT TCGACTATGA GTATGATGAA 
AATGGGGACA GAGTGGTTCT CGGTAAAGGG AAACATGGAG TGGTATATGC AGGAAGAGAC 
TTGAGTAACC AGGTCAGAAT TGCAATTAAA GAAGTTCCAG AGAGAGATGG TCGATGCTCT 
CAACCACTTC ATGAAGAGAT TGCTTTGCAT AAACGTCTAA AGCATAAAAA CATTGTTCAG 
TATTTGGGAT CATTCAGTAA AGGTGGTTTC ATTAAAATAT TTATGGAACA AGTACCTGGA 
GGAAGCCTCT CCGCACTTCT AAGGTCAAAA TGGGGACCCT TGAAAGACAA TGAGCAGACA 
ATCGGATTTT ATACCAAACA GATCCTTGAA GGTTTGAAAT ATCTCCATGA TAACCAGATA 
GTTCATAGAG ATATCAAGGG TGATAATGTT CTTATAAACA CATACAGTGG TGTTCTGAAA 
ATCTCTGATT TTGGCACTTC AAAGAGACTT GCTGGAATCA ACCCAAATAC AGAAACCTTC 
ACTGGCACTC TACAATATAT GGCACCAGAA ATTATAGACA AAGGACCAAG AGGATATGGA 
AAGCCAGCAG ACATTTGGTC TCTGGGATGC ACAGTTATAG AAATGGCAAC AGGAAAACCA 
CCATTTTATG AGCTTGGAGA ACCACAAGCT GCCATGTTTA AAGTGGGCAT GTTTAAAATT 
CATCCAGATA TCCCAGAATC TATGTCGTCT GAAGCTAAGG CATTTTTGTT GCGGTGCTTT 
GAACCAGATC CTGTGAAACG CGCTACTGCT ACTGAACTTA TAACGGATGA ATTCTTGAAG 
GTGTCCTCTA GGAAACGCAA GTCCCAGCTT AAGCTTACAA CTCTTTCCTC TGGCTCAAAT 
GAATATCTCC GTAGCATATC TTTGCCGGTG TCAGTGATGG TGGAAGATAC AAGCAGCAGC 
AGTGAATACA GTTCCGTTTC TCCAGATTCA GACTTAAGAT CGGACCCATT TACTTTTAAA 
ACAAGAGCCA GATCATGTAT AGAAAAGGAT GTCAAGCTTA ACAGAAGTTT ATTTCTAGGA 
ATTCCAGACG AGAATTTTGA AGACAGTAGT GCTCCTCCTT CTCCTGAAGA ACGAGACTCT 
GGATTTTTCA TGCTGAAGAA AGATAGTGAA AGGAGGGCTA CACTTTATCA TATTCTTACA 
GAGGATCAGG ACAAGGTGGC TAGAAACTTA ATGGATGCTT TAGTCCAGGG ATCAGAAATA 
AAATTGAAAT GGGACCACAT CACTGCTATT GTTGACAGCC TAAAAGAGTT TGTAAGATCT 
ACTGACAGGA AAATCATTTC AACAACACTA TCAAAACTCA AACTTGAGTT AGACTTTGAT 
AGTCATGCTA TCAGTCAAGT CCAGATGGTG CTGTTTGGCT TTCAAGATGC GGTAAATAAA 
GTGCTACGAA ATCATAACAT AAAGCCTCAC TGGATGTTTG CCTTAGATAA TATAATTCGA 
AAGGCCGTGC AGACAGCTAT CACTATCCTG GTACCTGGCT TTTGGAAGAA TTGTTACTTA 
AAGAGAGAGA ATTCCAAGTC CTTCTACGCC AAGCTATTGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k5 ( XM_012963758.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819212.1 Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa36964
Clone ID Related Accession (Same CDS sequence)	XM_012963758.3 , XM_012963758.2
Accession Version	XM_012963758.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963758.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTAA TGAGTGACAG CAGCGAAGGA ATCAGTTTCT CTGTACCATG CTCACGGCTG 
CCTGGCTCAA GCTGCTGTGC TCATGAAGAC ACAGGAGGGC TTACGCTACA GGAAAATGCC 
ACCATATGCG GTGGAACCAG GATCCCTGAA GCTGGAAGCT TCTGGCGTGA AGATTACCTC 
CGTGCTTCAG CGGTTGGAAG TGGTAAAGAT GCCCCATGCT ATGGAAAGTG GGTTAACCCC 
CCTCCTACAG CTAGCGGAGG TATTAGCCTT CGTAGGATTA CTGTGGTATA TGTTATCAAT 
GAAGTAAGTC CGGAGTTGCA TGCTTGTGAG GCTGCTGCAT TATACAGTTT GCAGGAGGCA 
TGTGAGAACC ATGGGGCACT GCTTGAGACC CTGGATTTCG GCAAACTGGA CTTTGGCGAG 
ACGTCAGTGC TAGACACCTT CTACAATGCA GATATTGCAG TGGTTGAGAT GAATGACGCT 
TTTCGACAGC CTTCTTTGTT CTACCATCTG GGAGTAAGAG AAAGCTTTAG CATGGCAAAC 
AACATAATTC TTTACTGTGA CACAAATTCA GACTCCCTGC AGTCACTGAA GGAAATTATC 
GGGCAAAAGA ATACTATGTG CACAGGTAAT TACACATTTA TTCCTTACAT GATCACGCCA 
CAAAACAAAG TGTTTTGCTG TGAGAGTATT TTCATGAGAG GAATGACTGA ACTAATGCCG 
TCAAACTTTG AGTTACTTCT TGGTCCGATC TGCCTGCCCC TAGTGGATCG CTTTGTTCAG 
ATTCTGAAGG TGGCACAGGC CACGTCATGC CAATATTTTC GAGAGGCAAT ACTAAATGAT 
ATTAGAAGGG CTCGGAACCA ATACACTGGG AAGGATCTGG CTGCTGAGCT AATAAGGATC 
CGCCAGCGCG TAGACAGCAT CGAGTTGTTA TCACCTGACA TCATTATCAA TCTGCTTTTG 
TCATACAGAG ATATCCAGGA CTACGATTCA GTTGTGACGT TGGTGGAAAT ATTGGAGAAA 
CTTCCAACCT TTAATTTATC TTCCAATCAC CATATAAAAT TTCATTATGC ATTTGCATTA 
AACAGGAGAA ATCATCCAGG TGACAGAGTA AAAGCCCTTG AAATAATGTT GCCTTTGGTG 
CAACTTGAAG ATCAAGTAGC TTCAGACATA TACTGCATTG TGGGAAGGAT CTACAAAGAT 
ATGTTCTTAG ATTCTAACTT CATGGATACC GAAAGTCGTG ATAATGCTAT ACATTGGTAC 
AGGAAGGCCT TTGAATCTGA ACCAACACTT CATTCTGGAA TCAATTATGC GGTGCTACTT 
CTAGCTGCTG GTCATCAATT TGAGACTTCT TTGGAACTCC GGAAAATCGG AGTTAAACTC 
AGTAGCCTGT TGGGTAAAAA AGGAAATCTC GAAAAACTCC AAAGTTATTG GGATGTTGGA 
TTTTATTTGG GGGCATGTAT ACTGGCCAAT GACTCATCAA AAGTTATCCA TGCAGCAGAA 
AAGCTTTTCA AGCTCAAAAC CCCAGCATGG TTCTTGAATT CAGTTGTGGA GACCATTTTG 
ATTTATCAGC ATTTTAAAAC GCCAAACCAA GAACAACATG TAACAAACCA TGAGCTGGTT 
GATTTTTGGA TGGATTTTCT TGTTGAGTCT ACAAGTAGCA ATGTGTCTGT ATTACGGTTT 
CCTGTCTTAA TACTAGAGCC AACAAAAATC TACCAGCCTT CCTACCTATC AATCAATAGT 
GAAGCTGAAG TTCAGACAAT ATCTATTTGG CACGTTCTAC CTGGCGATAA GAAAGGAATC 
CATGAATGGA ATTTCCCTGC AGATTCTATC AGAGGTGTAA GCATTTCAAA GTTTGAAGAA 
AGGTGCTGTT TTCTGTATGT TCTGAACAAC TCTGACGATT TTCAAATATA TTTCTGCACA 
GAGCTCCACT GCAAAAAATT CTTTGATAAA GTAAACCGTA TCAAAGAAGA GAATGGAAAG 
AACACAGAAG AAGGGGAGTG TGATGGAGAT TTAATGGAGT TCGACTATGA GTATGATGAA 
AATGGGGACA GAGTGGTTCT CGGTAAAGGG AAACATGGAG TGGTATATGC AGGAAGAGAC 
TTGAGTAACC AGGTCAGAAT TGCAATTAAA GAAGTTCCAG AGAGAGATGG TCGATGCTCT 
CAACCACTTC ATGAAGAGAT TGCTTTGCAT AAACGTCTAA AGCATAAAAA CATTGTTCAG 
TATTTGGGAT CATTCAGTAA AGGTGGTTTC ATTAAAATAT TTATGGAACA AGTACCTGGA 
GGAAGCCTCT CCGCACTTCT AAGGTCAAAA TGGGGACCCT TGAAAGACAA TGAGCAGACA 
ATCGGATTTT ATACCAAACA GATCCTTGAA GGTTTGAAAT ATCTCCATGA TAACCAGATA 
GTTCATAGAG ATATCAAGGG TGATAATGTT CTTATAAACA CATACAGTGG TGTTCTGAAA 
ATCTCTGATT TTGGCACTTC AAAGAGACTT GCTGGAATCA ACCCAAATAC AGAAACCTTC 
ACTGGCACTC TACAATATAT GGCACCAGAA ATTATAGACA AAGGACCAAG AGGATATGGA 
AAGCCAGCAG ACATTTGGTC TCTGGGATGC ACAGTTATAG AAATGGCAAC AGGAAAACCA 
CCATTTTATG AGCTTGGAGA ACCACAAGCT GCCATGTTTA AAGTGGGCAT GTTTAAAATT 
CATCCAGATA TCCCAGAATC TATGTCGTCT GAAGCTAAGG CATTTTTGTT GCGGTGCTTT 
GAACCAGATC CTGTGAAACG CGCTACTGCT ACTGAACTTA TAACGGATGA ATTCTTGAAG 
GTGTCCTCTA GGAAACGCAA GTCCCAGCTT AAGCTTACAA CTCTTTCCTC TGGCTCAAAT 
GAATATCTCC GTAGCATATC TTTGCCGGTG TCAGTGATGG TGGAAGATAC AAGCAGCAGC 
AGTGAATACA GTTCCGTTTC TCCAGATTCA GACTTAAGAT CGGACCCATT TACTTTTAAA 
ACAAGAGCCA GATCATGTAT AGAAAAGGAT GTCAAGCTTA ACAGAAGTTT ATTTCTAGGA 
ATTCCAGACG AGAATTTTGA AGACAGTAGT GCTCCTCCTT CTCCTGAAGA ACGAGACTCT 
GGATTTTTCA TGCTGAAGAA AGATAGTGAA AGGAGGGCTA CACTTTATCA TATTCTTACA 
GAGGATCAGG ACAAGGTGGC TAGAAACTTA ATGGATGCTT TAGTCCAGGG ATCAGAAATA 
AAATTGAAAT GGGACCACAT CACTGCTATT GTTGACAGCC TAAAAGAGTT TGTAAGATCT 
ACTGACAGGA AAATCATTTC AACAACACTA TCAAAACTCA AACTTGAGTT AGACTTTGAT 
AGTCATGCTA TCAGTCAAGT CCAGATGGTG CTGTTTGGCT TTCAAGATGC GGTAAATAAA 
GTGCTACGAA ATCATAACAT AAAGCCTCAC TGGATGTTTG CCTTAGATAA TATAATTCGA 
AAGGCCGTGC AGACAGCTAT CACTATCCTG GTACCTGAGC TGAGGCCACA TTTTAGCATT 
ACATCAGAAA GTGACCAAGC AGATCAGGAG GACATAGAAG TAGAGGAAAG AAGTAATAAA 
CAACCCACAG AACAAACCAT TCACAGAGAA GTCAATGAGG ACAACGTGCA AACCTCAGGA 
GTGAGCACAC TCAGTTCAAC TTTGTCCCAC AACTCTCAGA ATGCCGACAG GTCCCTGAAT 
GTGCAACTTG AAAGACTTAG GTTAGAGTCA AACAGGCTTT TGGAAGAATT GTTACTTAAA 
GAGAGAGAAT TCCAAGTCCT TCTACGCCAA GCTATTGAAG TGAAGGACAG GGAAATCAAA 
CGCCTGCAGT GCACGTCTGA GCCAATAAGC ACTTCCGTAT CCAGCACAAG GTCAACTATC 
TCAGGCAGTG AAGACTTGAA TCTTGTTTAC TGGCTACACA AGTGCAGAGT AGATGAAGAT 
ACAATAAGCA AGTTTTTGGC AGAAGACTAT CAGCTTGAAG ATATTCTTTG TCATGTCACA 
CGAGATGATT TAAAATCTCT GAATCTGAGG GGAGGAGTAC TATGCACAAT ATGGAAAGAA 
ATCACTGACT ACAGAGAAAA ACAAGCCTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819212.1
CDS	206..4318
Translation	MQVMSDSSEGISFSVPCSRLPGSSCCAHEDTGGLTLQENATICGGTRIPEAGSFWREDYLRASAVGSGKDAPCYGKWVNPPPTASGGISLRRITVVYVINEVSPELHACEAAALYSLQEACENHGALLETLDFGKLDFGETSVLDTFYNADIAVVEMNDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIIGQKNTMCTGNYTFIPYMITPQNKVFCCESIFMRGMTELMPSNFELLLGPICLPLVDRFVQILKVAQATSCQYFREAILNDIRRARNQYTGKDLAAELIRIRQRVDSIELLSPDIIINLLLSYRDIQDYDSVVTLVEILEKLPTFNLSSNHHIKFHYAFALNRRNHPGDRVKALEIMLPLVQLEDQVASDIYCIVGRIYKDMFLDSNFMDTESRDNAIHWYRKAFESEPTLHSGINYAVLLLAAGHQFETSLELRKIGVKLSSLLGKKGNLEKLQSYWDVGFYLGACILANDSSKVIHAAEKLFKLKTPAWFLNSVVETILIYQHFKTPNQEQHVTNHELVDFWMDFLVESTSSNVSVLRFPVLILEPTKIYQPSYLSINSEAEVQTISIWHVLPGDKKGIHEWNFPADSIRGVSISKFEERCCFLYVLNNSDDFQIYFCTELHCKKFFDKVNRIKEENGKNTEEGECDGDLMEFDYEYDENGDRVVLGKGKHGVVYAGRDLSNQVRIAIKEVPERDGRCSQPLHEEIALHKRLKHKNIVQYLGSFSKGGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPNTETFTGTLQYMAPEIIDKGPRGYGKPADIWSLGCTVIEMATGKPPFYELGEPQAAMFKVGMFKIHPDIPESMSSEAKAFLLRCFEPDPVKRATATELITDEFLKVSSRKRKSQLKLTTLSSGSNEYLRSISLPVSVMVEDTSSSSEYSSVSPDSDLRSDPFTFKTRARSCIEKDVKLNRSLFLGIPDENFEDSSAPPSPEERDSGFFMLKKDSERRATLYHILTEDQDKVARNLMDALVQGSEIKLKWDHITAIVDSLKEFVRSTDRKIISTTLSKLKLELDFDSHAISQVQMVLFGFQDAVNKVLRNHNIKPHWMFALDNIIRKAVQTAITILVPELRPHFSITSESDQADQEDIEVEERSNKQPTEQTIHREVNEDNVQTSGVSTLSSTLSHNSQNADRSLNVQLERLRLESNRLLEELLLKEREFQVLLRQAIEVKDREIKRLQCTSEPISTSVSSTRSTISGSEDLNLVYWLHKCRVDEDTISKFLAEDYQLEDILCHVTRDDLKSLNLRGGVLCTIWKEITDYREKQAS

Target ORF information:

RefSeq Version	XM_012963758.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963758.2

ORF Insert Sequence:

ATGCAAGTAA TGAGTGACAG CAGCGAAGGA ATCAGTTTCT CTGTACCATG CTCACGGCTG 
CCTGGCTCAA GCTGCTGTGC TCATGAAGAC ACAGGAGGGC TTACGCTACA GGAAAATGCC 
ACCATATGCG GTGGAACCAG GATCCCTGAA GCTGGAAGCT TCTGGCGTGA AGATTACCTC 
CGTGCTTCAG CGGTTGGAAG TGGTAAAGAT GCCCCATGCT ATGGAAAGTG GGTTAACCCC 
CCTCCTACAG CTAGCGGAGG TATTAGCCTT CGTAGGATTA CTGTGGTATA TGTTATCAAT 
GAAGTAAGTC CGGAGTTGCA TGCTTGTGAG GCTGCTGCAT TATACAGTTT GCAGGAGGCA 
TGTGAGAACC ATGGGGCACT GCTTGAGACC CTGGATTTCG GCAAACTGGA CTTTGGCGAG 
ACGTCAGTGC TAGACACCTT CTACAATGCA GATATTGCAG TGGTTGAGAT GAATGACGCT 
TTTCGACAGC CTTCTTTGTT CTACCATCTG GGAGTAAGAG AAAGCTTTAG CATGGCAAAC 
AACATAATTC TTTACTGTGA CACAAATTCA GACTCCCTGC AGTCACTGAA GGAAATTATC 
GGGCAAAAGA ATACTATGTG CACAGGTAAT TACACATTTA TTCCTTACAT GATCACGCCA 
CAAAACAAAG TGTTTTGCTG TGAGAGTATT TTCATGAGAG GAATGACTGA ACTAATGCCG 
TCAAACTTTG AGTTACTTCT TGGTCCGATC TGCCTGCCCC TAGTGGATCG CTTTGTTCAG 
ATTCTGAAGG TGGCACAGGC CACGTCATGC CAATATTTTC GAGAGGCAAT ACTAAATGAT 
ATTAGAAGGG CTCGGAACCA ATACACTGGG AAGGATCTGG CTGCTGAGCT AATAAGGATC 
CGCCAGCGCG TAGACAGCAT CGAGTTGTTA TCACCTGACA TCATTATCAA TCTGCTTTTG 
TCATACAGAG ATATCCAGGA CTACGATTCA GTTGTGACGT TGGTGGAAAT ATTGGAGAAA 
CTTCCAACCT TTAATTTATC TTCCAATCAC CATATAAAAT TTCATTATGC ATTTGCATTA 
AACAGGAGAA ATCATCCAGG TGACAGAGTA AAAGCCCTTG AAATAATGTT GCCTTTGGTG 
CAACTTGAAG ATCAAGTAGC TTCAGACATA TACTGCATTG TGGGAAGGAT CTACAAAGAT 
ATGTTCTTAG ATTCTAACTT CATGGATACC GAAAGTCGTG ATAATGCTAT ACATTGGTAC 
AGGAAGGCCT TTGAATCTGA ACCAACACTT CATTCTGGAA TCAATTATGC GGTGCTACTT 
CTAGCTGCTG GTCATCAATT TGAGACTTCT TTGGAACTCC GGAAAATCGG AGTTAAACTC 
AGTAGCCTGT TGGGTAAAAA AGGAAATCTC GAAAAACTCC AAAGTTATTG GGATGTTGGA 
TTTTATTTGG GGGCATGTAT ACTGGCCAAT GACTCATCAA AAGTTATCCA TGCAGCAGAA 
AAGCTTTTCA AGCTCAAAAC CCCAGCATGG TTCTTGAATT CAGTTGTGGA GACCATTTTG 
ATTTATCAGC ATTTTAAAAC GCCAAACCAA GAACAACATG TAACAAACCA TGAGCTGGTT 
GATTTTTGGA TGGATTTTCT TGTTGAGTCT ACAAGTAGCA ATGTGTCTGT ATTACGGTTT 
CCTGTCTTAA TACTAGAGCC AACAAAAATC TACCAGCCTT CCTACCTATC AATCAATAGT 
GAAGCTGAAG TTCAGACAAT ATCTATTTGG CACGTTCTAC CTGGCGATAA GAAAGGAATC 
CATGAATGGA ATTTCCCTGC AGATTCTATC AGAGGTGTAA GCATTTCAAA GTTTGAAGAA 
AGGTGCTGTT TTCTGTATGT TCTGAACAAC TCTGACGATT TTCAAATATA TTTCTGCACA 
GAGCTCCACT GCAAAAAATT CTTTGATAAA GTAAACCGTA TCAAAGAAGA GAATGGAAAG 
AACACAGAAG AAGGGGAGTG TGATGGAGAT TTAATGGAGT TCGACTATGA GTATGATGAA 
AATGGGGACA GAGTGGTTCT CGGTAAAGGG AAACATGGAG TGGTATATGC AGGAAGAGAC 
TTGAGTAACC AGGTCAGAAT TGCAATTAAA GAAGTTCCAG AGAGAGATGG TCGATGCTCT 
CAACCACTTC ATGAAGAGAT TGCTTTGCAT AAACGTCTAA AGCATAAAAA CATTGTTCAG 
TATTTGGGAT CATTCAGTAA AGGTGGTTTC ATTAAAATAT TTATGGAACA AGTACCTGGA 
GGAAGCCTCT CCGCACTTCT AAGGTCAAAA TGGGGACCCT TGAAAGACAA TGAGCAGACA 
ATCGGATTTT ATACCAAACA GATCCTTGAA GGTTTGAAAT ATCTCCATGA TAACCAGATA 
GTTCATAGAG ATATCAAGGG TGATAATGTT CTTATAAACA CATACAGTGG TGTTCTGAAA 
ATCTCTGATT TTGGCACTTC AAAGAGACTT GCTGGAATCA ACCCAAATAC AGAAACCTTC 
ACTGGCACTC TACAATATAT GGCACCAGAA ATTATAGACA AAGGACCAAG AGGATATGGA 
AAGCCAGCAG ACATTTGGTC TCTGGGATGC ACAGTTATAG AAATGGCAAC AGGAAAACCA 
CCATTTTATG AGCTTGGAGA ACCACAAGCT GCCATGTTTA AAGTGGGCAT GTTTAAAATT 
CATCCAGATA TCCCAGAATC TATGTCGTCT GAAGCTAAGG CATTTTTGTT GCGGTGCTTT 
GAACCAGATC CTGTGAAACG CGCTACTGCT ACTGAACTTA TAACGGATGA ATTCTTGAAG 
GTGTCCTCTA GGAAACGCAA GTCCCAGCTT AAGCTTACAA CTCTTTCCTC TGGCTCAAAT 
GAATATCTCC GTAGCATATC TTTGCCGGTG TCAGTGATGG TGGAAGATAC AAGCAGCAGC 
AGTGAATACA GTTCCGTTTC TCCAGATTCA GACTTAAGAT CGGACCCATT TACTTTTAAA 
ACAAGAGCCA GATCATGTAT AGAAAAGGAT GTCAAGCTTA ACAGAAGTTT ATTTCTAGGA 
ATTCCAGACG AGAATTTTGA AGACAGTAGT GCTCCTCCTT CTCCTGAAGA ACGAGACTCT 
GGATTTTTCA TGCTGAAGAA AGATAGTGAA AGGAGGGCTA CACTTTATCA TATTCTTACA 
GAGGATCAGG ACAAGGTGGC TAGAAACTTA ATGGATGCTT TAGTCCAGGG ATCAGAAATA 
AAATTGAAAT GGGACCACAT CACTGCTATT GTTGACAGCC TAAAAGAGTT TGTAAGATCT 
ACTGACAGGA AAATCATTTC AACAACACTA TCAAAACTCA AACTTGAGTT AGACTTTGAT 
AGTCATGCTA TCAGTCAAGT CCAGATGGTG CTGTTTGGCT TTCAAGATGC GGTAAATAAA 
GTGCTACGAA ATCATAACAT AAAGCCTCAC TGGATGTTTG CCTTAGATAA TATAATTCGA 
AAGGCCGTGC AGACAGCTAT CACTATCCTG GTACCTGAGC TGAGGCCACA TTTTAGCATT 
ACATCAGAAA GTGACCAAGC AGATCAGGAG GACATAGAAG TAGAGGAAAG AAGTAATAAA 
CAACCCACAG AACAAACCAT TCACAGAGAA GTCAATGAGG ACAACGTGCA AACCTCAGGA 
GTGAGCACAC TCAGTTCAAC TTTGTCCCAC AACTCTCAGA ATGCCGACAG GTCCCTGAAT 
GTGCAACTTG AAAGACTTAG GTTAGAGTCA AACAGGCTTT TGGAAGAATT GTTACTTAAA 
GAGAGAGAAT TCCAAGTCCT TCTACGCCAA GCTATTGAAG TGAAGGACAG GGAAATCAAA 
CGCCTGCAGT GCACGTCTGA GCCAATAAGC ACTTCCGTAT CCAGCACAAG GTCAACTATC 
TCAGGCAGTG AAGACTTGAA TCTTGTTTAC TGGCTACACA AGTGCAGAGT AGATGAAGAT 
ACAATAAGCA AGTTTTTGGC AGAAGACTAT CAGCTTGAAG ATATTCTTTG TCATGTCACA 
CGAGATGATT TAAAATCTCT GAATCTGAGG GGAGGAGTAC TATGCACAAT ATGGAAAGAA 
ATCACTGACT ACAGAGAAAA ACAAGCCTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k5 ( XM_012963759.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819213.1 Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X4, mRNA.

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CloneID	OXa36965
Clone ID Related Accession (Same CDS sequence)	XM_012963759.2 , XM_012963759.3
Accession Version	XM_012963759.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963759.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTAA TGAGTGACAG CAGCGAAGGA ATCAGTTTCT CTGTACCATG CTCACGGCTG 
CCTGGCTCAA GCTGCTGTGC TCATGAAGAC ACAGGAGGGC TTACGCTACA GGAAAATGCC 
ACCATATGCG GTGGAACCAG GATCCCTGAA GCTGGAAGCT TCTGGCGTGA AGATTACCTC 
CGTGCTTCAG CGGTTGGAAG TGGTAAAGAT GCCCCATGCT ATGGAAAGTG GGTTAACCCC 
CCTCCTACAG CTAGCGGAGG TATTAGCCTT CGTAGGATTA CTGTGGTATA TGTTATCAAT 
GAAGTAAGTC CGGAGTTGCA TGCTTGTGAG GCTGCTGCAT TATACAGTTT GCAGGAGGCA 
TGTGAGAACC ATGGGGCACT GCTTGAGACC CTGGATTTCG GCAAACTGGA CTTTGGCGAG 
ACGTCAGTGC TAGACACCTT CTACAATGCA GATATTGCAG TGGTTGAGAT GAATGACGCT 
TTTCGACAGC CTTCTTTGTT CTACCATCTG GGAGTAAGAG AAAGCTTTAG CATGGCAAAC 
AACATAATTC TTTACTGTGA CACAAATTCA GACTCCCTGC AGTCACTGAA GGAAATTATC 
GGGCAAAAGA ATACTATGTG CACAGGTAAT TACACATTTA TTCCTTACAT GATCACGCCA 
CAAAACAAAG TGTTTTGCTG TGAGAGTATT TTCATGAGAG GAATGACTGA ACTAATGCCG 
TCAAACTTTG AGTTACTTCT TGGTCCGATC TGCCTGCCCC TAGTGGATCG CTTTGTTCAG 
ATTCTGAAGG TGGCACAGGC CACGTCATGC CAATATTTTC GAGAGGCAAT ACTAAATGAT 
ATTAGAAGGG CTCGGAACCA ATACACTGGG AAGGATCTGG CTGCTGAGCT AATAAGGATC 
CGCCAGCGCG TAGACAGCAT CGAGTTGTTA TCACCTGACA TCATTATCAA TCTGCTTTTG 
TCATACAGAG ATATCCAGGA CTACGATTCA GTTGTGACGT TGGTGGAAAT ATTGGAGAAA 
CTTCCAACCT TTAATTTATC TTCCAATCAC CATATAAAAT TTCATTATGC ATTTGCATTA 
AACAGGAGAA ATCATCCAGG TGACAGAGTA AAAGCCCTTG AAATAATGTT GCCTTTGGTG 
CAACTTGAAG ATCAAGTAGC TTCAGACATA TACTGCATTG TGGGAAGGAT CTACAAAGAT 
ATGTTCTTAG ATTCTAACTT CATGGATACC GAAAGTCGTG ATAATGCTAT ACATTGGTAC 
AGGAAGGCCT TTGAATCTGA ACCAACACTT CATTCTGGAA TCAATTATGC GGTGCTACTT 
CTAGCTGCTG GTCATCAATT TGAGACTTCT TTGGAACTCC GGAAAATCGG AGTTAAACTC 
AGTAGCCTGT TGGGTAAAAA AGGAAATCTC GAAAAACTCC AAAGTTATTG GGATGTTGGA 
TTTTATTTGG GGGCATGTAT ACTGGCCAAT GACTCATCAA AAGTTATCCA TGCAGCAGAA 
AAGCTTTTCA AGCTCAAAAC CCCAGCATGG TTCTTGAATT CAGTTGTGGA GACCATTTTG 
ATTTATCAGC ATTTTAAAAC GCCAAACCAA GAACAACATG TAACAAACCA TGAGCTGGTT 
GATTTTTGGA TGGATTTTCT TGTTGAGTCT ACAAGTAGCA ATGTGTCTGT ATTACGGTTT 
CCTGTCTTAA TACTAGAGCC AACAAAAATC TACCAGCCTT CCTACCTATC AATCAATAGT 
GAAGCTGAAG TTCAGACAAT ATCTATTTGG CACGTTCTAC CTGGCGATAA GAAAGGAATC 
CATGAATGGA ATTTCCCTGC AGATTCTATC AGAGGTGTAA GCATTTCAAA GTTTGAAGAA 
AGGTGCTGTT TTCTGTATGT TCTGAACAAC TCTGACGATT TTCAAATATA TTTCTGCACA 
GAGCTCCACT GCAAAAAATT CTTTGATAAA GTAAACCGTA TCAAAGAAGA GAATGGAAAG 
AACACAGAAG AAGGGGAGTG TGATGGAGAT TTAATGGAGT TCGACTATGA GTATGATGAA 
AATGGGGACA GAGTGGTTCT CGGTAAAGGG AAACATGGAG TGGTATATGC AGGAAGAGAC 
TTGAGTAACC AGGTCAGAAT TGCAATTAAA GAAGTTCCAG AGAGAGATGG TCGATGCTCT 
CAACCACTTC ATGAAGAGAT TGCTTTGCAT AAACGTCTAA AGCATAAAAA CATTGTTCAG 
TATTTGGGAT CATTCAGTAA AGGTGGTTTC ATTAAAATAT TTATGGAACA AGTACCTGGA 
GGAAGCCTCT CCGCACTTCT AAGGTCAAAA TGGGGACCCT TGAAAGACAA TGAGCAGACA 
ATCGGATTTT ATACCAAACA GATCCTTGAA GGTTTGAAAT ATCTCCATGA TAACCAGATA 
GTTCATAGAG ATATCAAGGG TGATAATGTT CTTATAAACA CATACAGTGG TGTTCTGAAA 
ATCTCTGATT TTGGCACTTC AAAGAGACTT GCTGGAATCA ACCCAAATAC AGAAACCTTC 
ACTGGCACTC TACAATATAT GGCACCAGAA ATTATAGACA AAGGACCAAG AGGATATGGA 
AAGCCAGCAG ACATTTGGTC TCTGGGATGC ACAGTTATAG AAATGGCAAC AGGAAAACCA 
CCATTTTATG AGCTTGGAGA ACCACAAGCT GCCATGTTTA AAGTGGGCAT GTTTAAAATT 
CATCCAGATA TCCCAGAATC TATGTCGTCT GAAGCTAAGG CATTTTTGTT GCGGTGCTTT 
GAACCAGATC CTGTGAAACG CGCTACTGCT ACTGAACTTA TAACGGATGA ATTCTTGAAG 
GTGTCCTCTA GGAAACGCAA GTCCCAGCTT AAGCTTACAA CTCTTTCCTC TGGCTCAAAT 
GAATATCTCC GTAGCATATC TTTGCCGGTG TCAGTGATGG TGGAAGATAC AAGCAGCAGC 
AGTGAATACA GTTCCGTTTC TCCAGATTCA GACTTAAGAT CGGACCCATT TACTTTTAAA 
ACAAGAGCCA GATCATGTAT AGAAAAGGAT GTCAAGCTTA ACAGAAGTTT ATTTCTAGGA 
ATTCCAGACG AGAATTTTGA AGACAGTAGT GCTCCTCCTT CTCCTGAAGA ACGAGACTCT 
GGATTTTTCA TGCTGAAGAA AGATAGTGAA AGGAGGGCTA CACTTTATCA TATTCTTACA 
GAGGATCAGG ACAAGGTGGC TAGAAACTTA ATGGATGCTT TAGTCCAGGG ATCAGAAATA 
AAATTGAAAT GGGACCACAT CACTGCTATT GTTGACAGCC TAAAAGAGTT TGTAAGATCT 
ACTGACAGGA AAATCATTTC AACAACACTA TCAAAACTCA AACTTGAGTT AGACTTTGAT 
AGTCATGCTA TCAGTCAAGT CCAGATGGTG CTGTTTGGCT TTCAAGATGC GGTAAATAAA 
GTGCTACGAA ATCATAACAT AAAGCCTCAC TGGATGTTTG CCTTAGATAA TATAATTCGA 
AAGGCCGTGC AGACAGCTAT CACTATCCTG GTACCTGAGC TGAGGCCACA TTTTAGCATT 
ACATCAGAAA GTGACCAAGC AGATCAGGAG GACATAGAAG TAGAGGAAAG AAGTAATAAA 
CAACCCACAG AACAAACCAT TCACAGAGAA GTCAATGAGG ACAACGTGCA AACCTCAGGA 
GTGAGCACAC TCAGTTCAAC TTTGTCCCAC AACTCTCAGA ATGCCGACAG GTCCCTGAAT 
GTGCAACTTG AAAGACTTAG GTTAGAGTCA AACAGGCTTT TGGAAGAATT GTTACTTAAA 
GAGAGAGAAT TCCAAGTCCT TCTACGCCAA GCTATTGAAG TGAAGGACAG GGAAATCAAA 
CGCCTGCAGT GCACGTCTGA GCCAATAAGC ACTTCCGTAT CCAGCACAAG GTCAACTATC 
TCAGGCAGTG AAGACTTGAA TCTTGTTTAC TGGCTACACA AGTGCAGAGT AGATGAAGAT 
ACAATAAGCA AGGGGAGGAG TACTATGCAC AATATGGAAA GAAATCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819213.1
CDS	207..4217
Translation	MQVMSDSSEGISFSVPCSRLPGSSCCAHEDTGGLTLQENATICGGTRIPEAGSFWREDYLRASAVGSGKDAPCYGKWVNPPPTASGGISLRRITVVYVINEVSPELHACEAAALYSLQEACENHGALLETLDFGKLDFGETSVLDTFYNADIAVVEMNDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIIGQKNTMCTGNYTFIPYMITPQNKVFCCESIFMRGMTELMPSNFELLLGPICLPLVDRFVQILKVAQATSCQYFREAILNDIRRARNQYTGKDLAAELIRIRQRVDSIELLSPDIIINLLLSYRDIQDYDSVVTLVEILEKLPTFNLSSNHHIKFHYAFALNRRNHPGDRVKALEIMLPLVQLEDQVASDIYCIVGRIYKDMFLDSNFMDTESRDNAIHWYRKAFESEPTLHSGINYAVLLLAAGHQFETSLELRKIGVKLSSLLGKKGNLEKLQSYWDVGFYLGACILANDSSKVIHAAEKLFKLKTPAWFLNSVVETILIYQHFKTPNQEQHVTNHELVDFWMDFLVESTSSNVSVLRFPVLILEPTKIYQPSYLSINSEAEVQTISIWHVLPGDKKGIHEWNFPADSIRGVSISKFEERCCFLYVLNNSDDFQIYFCTELHCKKFFDKVNRIKEENGKNTEEGECDGDLMEFDYEYDENGDRVVLGKGKHGVVYAGRDLSNQVRIAIKEVPERDGRCSQPLHEEIALHKRLKHKNIVQYLGSFSKGGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPNTETFTGTLQYMAPEIIDKGPRGYGKPADIWSLGCTVIEMATGKPPFYELGEPQAAMFKVGMFKIHPDIPESMSSEAKAFLLRCFEPDPVKRATATELITDEFLKVSSRKRKSQLKLTTLSSGSNEYLRSISLPVSVMVEDTSSSSEYSSVSPDSDLRSDPFTFKTRARSCIEKDVKLNRSLFLGIPDENFEDSSAPPSPEERDSGFFMLKKDSERRATLYHILTEDQDKVARNLMDALVQGSEIKLKWDHITAIVDSLKEFVRSTDRKIISTTLSKLKLELDFDSHAISQVQMVLFGFQDAVNKVLRNHNIKPHWMFALDNIIRKAVQTAITILVPELRPHFSITSESDQADQEDIEVEERSNKQPTEQTIHREVNEDNVQTSGVSTLSSTLSHNSQNADRSLNVQLERLRLESNRLLEELLLKEREFQVLLRQAIEVKDREIKRLQCTSEPISTSVSSTRSTISGSEDLNLVYWLHKCRVDEDTISKGRSTMHNMERNH

Target ORF information:

RefSeq Version	XM_012963759.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963759.2

ORF Insert Sequence:

ATGCAAGTAA TGAGTGACAG CAGCGAAGGA ATCAGTTTCT CTGTACCATG CTCACGGCTG 
CCTGGCTCAA GCTGCTGTGC TCATGAAGAC ACAGGAGGGC TTACGCTACA GGAAAATGCC 
ACCATATGCG GTGGAACCAG GATCCCTGAA GCTGGAAGCT TCTGGCGTGA AGATTACCTC 
CGTGCTTCAG CGGTTGGAAG TGGTAAAGAT GCCCCATGCT ATGGAAAGTG GGTTAACCCC 
CCTCCTACAG CTAGCGGAGG TATTAGCCTT CGTAGGATTA CTGTGGTATA TGTTATCAAT 
GAAGTAAGTC CGGAGTTGCA TGCTTGTGAG GCTGCTGCAT TATACAGTTT GCAGGAGGCA 
TGTGAGAACC ATGGGGCACT GCTTGAGACC CTGGATTTCG GCAAACTGGA CTTTGGCGAG 
ACGTCAGTGC TAGACACCTT CTACAATGCA GATATTGCAG TGGTTGAGAT GAATGACGCT 
TTTCGACAGC CTTCTTTGTT CTACCATCTG GGAGTAAGAG AAAGCTTTAG CATGGCAAAC 
AACATAATTC TTTACTGTGA CACAAATTCA GACTCCCTGC AGTCACTGAA GGAAATTATC 
GGGCAAAAGA ATACTATGTG CACAGGTAAT TACACATTTA TTCCTTACAT GATCACGCCA 
CAAAACAAAG TGTTTTGCTG TGAGAGTATT TTCATGAGAG GAATGACTGA ACTAATGCCG 
TCAAACTTTG AGTTACTTCT TGGTCCGATC TGCCTGCCCC TAGTGGATCG CTTTGTTCAG 
ATTCTGAAGG TGGCACAGGC CACGTCATGC CAATATTTTC GAGAGGCAAT ACTAAATGAT 
ATTAGAAGGG CTCGGAACCA ATACACTGGG AAGGATCTGG CTGCTGAGCT AATAAGGATC 
CGCCAGCGCG TAGACAGCAT CGAGTTGTTA TCACCTGACA TCATTATCAA TCTGCTTTTG 
TCATACAGAG ATATCCAGGA CTACGATTCA GTTGTGACGT TGGTGGAAAT ATTGGAGAAA 
CTTCCAACCT TTAATTTATC TTCCAATCAC CATATAAAAT TTCATTATGC ATTTGCATTA 
AACAGGAGAA ATCATCCAGG TGACAGAGTA AAAGCCCTTG AAATAATGTT GCCTTTGGTG 
CAACTTGAAG ATCAAGTAGC TTCAGACATA TACTGCATTG TGGGAAGGAT CTACAAAGAT 
ATGTTCTTAG ATTCTAACTT CATGGATACC GAAAGTCGTG ATAATGCTAT ACATTGGTAC 
AGGAAGGCCT TTGAATCTGA ACCAACACTT CATTCTGGAA TCAATTATGC GGTGCTACTT 
CTAGCTGCTG GTCATCAATT TGAGACTTCT TTGGAACTCC GGAAAATCGG AGTTAAACTC 
AGTAGCCTGT TGGGTAAAAA AGGAAATCTC GAAAAACTCC AAAGTTATTG GGATGTTGGA 
TTTTATTTGG GGGCATGTAT ACTGGCCAAT GACTCATCAA AAGTTATCCA TGCAGCAGAA 
AAGCTTTTCA AGCTCAAAAC CCCAGCATGG TTCTTGAATT CAGTTGTGGA GACCATTTTG 
ATTTATCAGC ATTTTAAAAC GCCAAACCAA GAACAACATG TAACAAACCA TGAGCTGGTT 
GATTTTTGGA TGGATTTTCT TGTTGAGTCT ACAAGTAGCA ATGTGTCTGT ATTACGGTTT 
CCTGTCTTAA TACTAGAGCC AACAAAAATC TACCAGCCTT CCTACCTATC AATCAATAGT 
GAAGCTGAAG TTCAGACAAT ATCTATTTGG CACGTTCTAC CTGGCGATAA GAAAGGAATC 
CATGAATGGA ATTTCCCTGC AGATTCTATC AGAGGTGTAA GCATTTCAAA GTTTGAAGAA 
AGGTGCTGTT TTCTGTATGT TCTGAACAAC TCTGACGATT TTCAAATATA TTTCTGCACA 
GAGCTCCACT GCAAAAAATT CTTTGATAAA GTAAACCGTA TCAAAGAAGA GAATGGAAAG 
AACACAGAAG AAGGGGAGTG TGATGGAGAT TTAATGGAGT TCGACTATGA GTATGATGAA 
AATGGGGACA GAGTGGTTCT CGGTAAAGGG AAACATGGAG TGGTATATGC AGGAAGAGAC 
TTGAGTAACC AGGTCAGAAT TGCAATTAAA GAAGTTCCAG AGAGAGATGG TCGATGCTCT 
CAACCACTTC ATGAAGAGAT TGCTTTGCAT AAACGTCTAA AGCATAAAAA CATTGTTCAG 
TATTTGGGAT CATTCAGTAA AGGTGGTTTC ATTAAAATAT TTATGGAACA AGTACCTGGA 
GGAAGCCTCT CCGCACTTCT AAGGTCAAAA TGGGGACCCT TGAAAGACAA TGAGCAGACA 
ATCGGATTTT ATACCAAACA GATCCTTGAA GGTTTGAAAT ATCTCCATGA TAACCAGATA 
GTTCATAGAG ATATCAAGGG TGATAATGTT CTTATAAACA CATACAGTGG TGTTCTGAAA 
ATCTCTGATT TTGGCACTTC AAAGAGACTT GCTGGAATCA ACCCAAATAC AGAAACCTTC 
ACTGGCACTC TACAATATAT GGCACCAGAA ATTATAGACA AAGGACCAAG AGGATATGGA 
AAGCCAGCAG ACATTTGGTC TCTGGGATGC ACAGTTATAG AAATGGCAAC AGGAAAACCA 
CCATTTTATG AGCTTGGAGA ACCACAAGCT GCCATGTTTA AAGTGGGCAT GTTTAAAATT 
CATCCAGATA TCCCAGAATC TATGTCGTCT GAAGCTAAGG CATTTTTGTT GCGGTGCTTT 
GAACCAGATC CTGTGAAACG CGCTACTGCT ACTGAACTTA TAACGGATGA ATTCTTGAAG 
GTGTCCTCTA GGAAACGCAA GTCCCAGCTT AAGCTTACAA CTCTTTCCTC TGGCTCAAAT 
GAATATCTCC GTAGCATATC TTTGCCGGTG TCAGTGATGG TGGAAGATAC AAGCAGCAGC 
AGTGAATACA GTTCCGTTTC TCCAGATTCA GACTTAAGAT CGGACCCATT TACTTTTAAA 
ACAAGAGCCA GATCATGTAT AGAAAAGGAT GTCAAGCTTA ACAGAAGTTT ATTTCTAGGA 
ATTCCAGACG AGAATTTTGA AGACAGTAGT GCTCCTCCTT CTCCTGAAGA ACGAGACTCT 
GGATTTTTCA TGCTGAAGAA AGATAGTGAA AGGAGGGCTA CACTTTATCA TATTCTTACA 
GAGGATCAGG ACAAGGTGGC TAGAAACTTA ATGGATGCTT TAGTCCAGGG ATCAGAAATA 
AAATTGAAAT GGGACCACAT CACTGCTATT GTTGACAGCC TAAAAGAGTT TGTAAGATCT 
ACTGACAGGA AAATCATTTC AACAACACTA TCAAAACTCA AACTTGAGTT AGACTTTGAT 
AGTCATGCTA TCAGTCAAGT CCAGATGGTG CTGTTTGGCT TTCAAGATGC GGTAAATAAA 
GTGCTACGAA ATCATAACAT AAAGCCTCAC TGGATGTTTG CCTTAGATAA TATAATTCGA 
AAGGCCGTGC AGACAGCTAT CACTATCCTG GTACCTGAGC TGAGGCCACA TTTTAGCATT 
ACATCAGAAA GTGACCAAGC AGATCAGGAG GACATAGAAG TAGAGGAAAG AAGTAATAAA 
CAACCCACAG AACAAACCAT TCACAGAGAA GTCAATGAGG ACAACGTGCA AACCTCAGGA 
GTGAGCACAC TCAGTTCAAC TTTGTCCCAC AACTCTCAGA ATGCCGACAG GTCCCTGAAT 
GTGCAACTTG AAAGACTTAG GTTAGAGTCA AACAGGCTTT TGGAAGAATT GTTACTTAAA 
GAGAGAGAAT TCCAAGTCCT TCTACGCCAA GCTATTGAAG TGAAGGACAG GGAAATCAAA 
CGCCTGCAGT GCACGTCTGA GCCAATAAGC ACTTCCGTAT CCAGCACAAG GTCAACTATC 
TCAGGCAGTG AAGACTTGAA TCTTGTTTAC TGGCTACACA AGTGCAGAGT AGATGAAGAT 
ACAATAAGCA AGGGGAGGAG TACTATGCAC AATATGGAAA GAAATCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k5 ( XM_012963762.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819216.1 Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X8, mRNA.

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CloneID	OXa36968
Clone ID Related Accession (Same CDS sequence)	XM_012963762.2 , XM_012963762.3
Accession Version	XM_012963762.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963762.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTAA TGAGTGACAG CAGCGAAGGA ATCAGTTTCT CTGTACCATG CTCACGGCTG 
CCTGGCTCAA GCTGCTGTGC TCATGAAGAC ACAGGAGGGC TTACGCTACA GGAAAATGCC 
ACCATATGCG GTGGAACCAG GATCCCTGAA GCTGGAAGCT TCTGGCGTGA AGATTACCTC 
CGTGCTTCAG CGGTTGGAAG TGGTAAAGAT GCCCCATGCT ATGGAAAGTG GGTTAACCCC 
CCTCCTACAG CTAGCGGAGG TATTAGCCTT CGTAGGATTA CTGTGGTATA TGTTATCAAT 
GAAGTAAGTC CGGAGTTGCA TGCTTGTGAG GCTGCTGCAT TATACAGTTT GCAGGAGGCA 
TGTGAGAACC ATGGGGCACT GCTTGAGACC CTGGATTTCG GCAAACTGGA CTTTGGCGAG 
ACGTCAGTGC TAGACACCTT CTACAATGCA GATATTGCAG TGGTTGAGAT GAATGACGCT 
TTTCGACAGC CTTCTTTGTT CTACCATCTG GGAGTAAGAG AAAGCTTTAG CATGGCAAAC 
AACATAATTC TTTACTGTGA CACAAATTCA GACTCCCTGC AGTCACTGAA GATGTGCACA 
GGTAATTACA CATTTATTCC TTACATGATC ACGCCACAAA ACAAAGTGTT TTGCTGTGAG 
AGTATTTTCA TGAGAGGAAT GACTGAACTA ATGCCGTCAA ACTTTGAGTT ACTTCTTGGT 
CCGATCTGCC TGCCCCTAGT GGATCGCTTT GTTCAGATTC TGAAGGTGGC ACAGGCCACG 
TCATGCCAAT ATTTTCGAGA GGCAATACTA AATGATATTA GAAGGGCTCG GAACCAATAC 
ACTGGGAAGG ATCTGGCTGC TGAGCTAATA AGGATCCGCC AGCGCGTAGA CAGCATCGAG 
TTGTTATCAC CTGACATCAT TATCAATCTG CTTTTGTCAT ACAGAGATAT CCAGGACTAC 
GATTCAGTTG TGACGTTGGT GGAAATATTG GAGAAACTTC CAACCTTTAA TTTATCTTCC 
AATCACCATA TAAAATTTCA TTATGCATTT GCATTAAACA GGAGAAATCA TCCAGGTGAC 
AGAGTAAAAG CCCTTGAAAT AATGTTGCCT TTGGTGCAAC TTGAAGATCA AGTAGCTTCA 
GACATATACT GCATTGTGGG AAGGATCTAC AAAGATATGT TCTTAGATTC TAACTTCATG 
GATACCGAAA GTCGTGATAA TGCTATACAT TGGTACAGGA AGGCCTTTGA ATCTGAACCA 
ACACTTCATT CTGGAATCAA TTATGCGGTG CTACTTCTAG CTGCTGGTCA TCAATTTGAG 
ACTTCTTTGG AACTCCGGAA AATCGGAGTT AAACTCAGTA GCCTGTTGGG TAAAAAAGGA 
AATCTCGAAA AACTCCAAAG TTATTGGGAT GTTGGATTTT ATTTGGGGGC ATGTATACTG 
GCCAATGACT CATCAAAAGT TATCCATGCA GCAGAAAAGC TTTTCAAGCT CAAAACCCCA 
GCATGGTTCT TGAATTCAGT TGTGGAGACC ATTTTGATTT ATCAGCATTT TAAAACGCCA 
AACCAAGAAC AACATGTAAC AAACCATGAG CTGGTTGATT TTTGGATGGA TTTTCTTGTT 
GAGTCTACAA GTAGCAATGT GTCTGTATTA CGGTTTCCTG TCTTAATACT AGAGCCAACA 
AAAATCTACC AGCCTTCCTA CCTATCAATC AATAGTGAAG CTGAAGTTCA GACAATATCT 
ATTTGGCACG TTCTACCTGG CGATAAGAAA GGAATCCATG AATGGAATTT CCCTGCAGAT 
TCTATCAGAG GTGTAAGCAT TTCAAAGTTT GAAGAAAGGT GCTGTTTTCT GTATGTTCTG 
AACAACTCTG ACGATTTTCA AATATATTTC TGCACAGAGC TCCACTGCAA AAAATTCTTT 
GATAAAGTAA ACCGTATCAA AGAAGAGAAT GGAAAGAACA CAGAAGAAGG GGAGTGTGAT 
GGAGATTTAA TGGAGTTCGA CTATGAGTAT GATGAAAATG GGGACAGAGT GGTTCTCGGT 
AAAGGGAAAC ATGGAGTGGT ATATGCAGGA AGAGACTTGA GTAACCAGGT CAGAATTGCA 
ATTAAAGAAG TTCCAGAGAG AGATGGTCGA TGCTCTCAAC CACTTCATGA AGAGATTGCT 
TTGCATAAAC GTCTAAAGCA TAAAAACATT GTTCAGTATT TGGGATCATT CAGTAAAGGT 
GGTTTCATTA AAATATTTAT GGAACAAGTA CCTGGAGGAA GCCTCTCCGC ACTTCTAAGG 
TCAAAATGGG GACCCTTGAA AGACAATGAG CAGACAATCG GATTTTATAC CAAACAGATC 
CTTGAAGGTT TGAAATATCT CCATGATAAC CAGATAGTTC ATAGAGATAT CAAGGGTGAT 
AATGTTCTTA TAAACACATA CAGTGGTGTT CTGAAAATCT CTGATTTTGG CACTTCAAAG 
AGACTTGCTG GAATCAACCC AAATACAGAA ACCTTCACTG GCACTCTACA ATATATGGCA 
CCAGAAATTA TAGACAAAGG ACCAAGAGGA TATGGAAAGC CAGCAGACAT TTGGTCTCTG 
GGATGCACAG TTATAGAAAT GGCAACAGGA AAACCACCAT TTTATGAGCT TGGAGAACCA 
CAAGCTGCCA TGTTTAAAGT GGGCATGTTT AAAATTCATC CAGATATCCC AGAATCTATG 
TCGTCTGAAG CTAAGGCATT TTTGTTGCGG TGCTTTGAAC CAGATCCTGT GAAACGCGCT 
ACTGCTACTG AACTTATAAC GGATGAATTC TTGAAGGTGT CCTCTAGGAA ACGCAAGTCC 
CAGCTTAAGC TTACAACTCT TTCCTCTGGC TCAAATGAAT ATCTCCGTAG CATATCTTTG 
CCGGTGTCAG TGATGGTGGA AGATACAAGC AGCAGCAGTG AATACAGTTC CGTTTCTCCA 
GATTCAGACT TAAGATCGGA CCCATTTACT TTTAAAACAA GAGCCAGATC ATGTATAGAA 
AAGGATGTCA AGCTTAACAG AAGTTTATTT CTAGGAATTC CAGACGAGAA TTTTGAAGAC 
AGTAGTGCTC CTCCTTCTCC TGAAGAACGA GACTCTGGAT TTTTCATGCT GAAGAAAGAT 
AGTGAAAGGA GGGCTACACT TTATCATATT CTTACAGAGG ATCAGGACAA GGTGGCTAGA 
AACTTAATGG ATGCTTTAGT CCAGGGATCA GAAATAAAAT TGAAATGGGA CCACATCACT 
GCTATTGTTG ACAGCCTAAA AGAGTTTGTA AGATCTACTG ACAGGAAAAT CATTTCAACA 
ACACTATCAA AACTCAAACT TGAGTTAGAC TTTGATAGTC ATGCTATCAG TCAAGTCCAG 
ATGGTGCTGT TTGGCTTTCA AGATGCGGTA AATAAAGTGC TACGAAATCA TAACATAAAG 
CCTCACTGGA TGTTTGCCTT AGATAATATA ATTCGAAAGG CCGTGCAGAC AGCTATCACT 
ATCCTGGTAC CTGAGCTGAG GCCACATTTT AGCATTACAT CAGAAAGTGA CCAAGCAGAT 
CAGGAGGACA TAGAAGTAGA GGAAAGAAGT AATAAACAAC CCACAGAACA AACCATTCAC 
AGAGAAGTCA ATGAGGACAA CGTGCAAACC TCAGGAGTGA GCACACTCAG TTCAACTTTG 
TCCCACAACT CTCAGAATGC CGACAGGTCC CTGAATGTGC AACTTGAAAG ACTTAGGTTA 
GAGTCAAACA GGCTTTTGGA AGAATTGTTA CTTAAAGAGA GAGAATTCCA AGTCCTTCTA 
CGCCAAGCTA TTGAAGTGAA GGACAGGGAA ATCAAACGCC TGCAGTGCAC GTCTGAGCCA 
ATAAGCACTT CCGTATCCAG CACAAGGTCA ACTATCTCAG GCAGTGAAGA CTTGAATCTT 
GTTTACTGGC TACACAAGTG CAGAGTAGAT GAAGATACAA TAAGCAAGTT TTTGGCAGAA 
GACTATCAGC TTGAAGATAT TCTTTGTCAT GTCACACGAG ATGATTTAAA ATCTCTGAAT 
CTGAGGGGAG GAGTACTATG CACAATATGG AAAGAAATCA CTGACTACAG AGAAAAACAA 
GCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819216.1
CDS	207..4295
Translation	MQVMSDSSEGISFSVPCSRLPGSSCCAHEDTGGLTLQENATICGGTRIPEAGSFWREDYLRASAVGSGKDAPCYGKWVNPPPTASGGISLRRITVVYVINEVSPELHACEAAALYSLQEACENHGALLETLDFGKLDFGETSVLDTFYNADIAVVEMNDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKMCTGNYTFIPYMITPQNKVFCCESIFMRGMTELMPSNFELLLGPICLPLVDRFVQILKVAQATSCQYFREAILNDIRRARNQYTGKDLAAELIRIRQRVDSIELLSPDIIINLLLSYRDIQDYDSVVTLVEILEKLPTFNLSSNHHIKFHYAFALNRRNHPGDRVKALEIMLPLVQLEDQVASDIYCIVGRIYKDMFLDSNFMDTESRDNAIHWYRKAFESEPTLHSGINYAVLLLAAGHQFETSLELRKIGVKLSSLLGKKGNLEKLQSYWDVGFYLGACILANDSSKVIHAAEKLFKLKTPAWFLNSVVETILIYQHFKTPNQEQHVTNHELVDFWMDFLVESTSSNVSVLRFPVLILEPTKIYQPSYLSINSEAEVQTISIWHVLPGDKKGIHEWNFPADSIRGVSISKFEERCCFLYVLNNSDDFQIYFCTELHCKKFFDKVNRIKEENGKNTEEGECDGDLMEFDYEYDENGDRVVLGKGKHGVVYAGRDLSNQVRIAIKEVPERDGRCSQPLHEEIALHKRLKHKNIVQYLGSFSKGGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPNTETFTGTLQYMAPEIIDKGPRGYGKPADIWSLGCTVIEMATGKPPFYELGEPQAAMFKVGMFKIHPDIPESMSSEAKAFLLRCFEPDPVKRATATELITDEFLKVSSRKRKSQLKLTTLSSGSNEYLRSISLPVSVMVEDTSSSSEYSSVSPDSDLRSDPFTFKTRARSCIEKDVKLNRSLFLGIPDENFEDSSAPPSPEERDSGFFMLKKDSERRATLYHILTEDQDKVARNLMDALVQGSEIKLKWDHITAIVDSLKEFVRSTDRKIISTTLSKLKLELDFDSHAISQVQMVLFGFQDAVNKVLRNHNIKPHWMFALDNIIRKAVQTAITILVPELRPHFSITSESDQADQEDIEVEERSNKQPTEQTIHREVNEDNVQTSGVSTLSSTLSHNSQNADRSLNVQLERLRLESNRLLEELLLKEREFQVLLRQAIEVKDREIKRLQCTSEPISTSVSSTRSTISGSEDLNLVYWLHKCRVDEDTISKFLAEDYQLEDILCHVTRDDLKSLNLRGGVLCTIWKEITDYREKQAS

Target ORF information:

RefSeq Version	XM_012963762.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963762.2

ORF Insert Sequence:

ATGCAAGTAA TGAGTGACAG CAGCGAAGGA ATCAGTTTCT CTGTACCATG CTCACGGCTG 
CCTGGCTCAA GCTGCTGTGC TCATGAAGAC ACAGGAGGGC TTACGCTACA GGAAAATGCC 
ACCATATGCG GTGGAACCAG GATCCCTGAA GCTGGAAGCT TCTGGCGTGA AGATTACCTC 
CGTGCTTCAG CGGTTGGAAG TGGTAAAGAT GCCCCATGCT ATGGAAAGTG GGTTAACCCC 
CCTCCTACAG CTAGCGGAGG TATTAGCCTT CGTAGGATTA CTGTGGTATA TGTTATCAAT 
GAAGTAAGTC CGGAGTTGCA TGCTTGTGAG GCTGCTGCAT TATACAGTTT GCAGGAGGCA 
TGTGAGAACC ATGGGGCACT GCTTGAGACC CTGGATTTCG GCAAACTGGA CTTTGGCGAG 
ACGTCAGTGC TAGACACCTT CTACAATGCA GATATTGCAG TGGTTGAGAT GAATGACGCT 
TTTCGACAGC CTTCTTTGTT CTACCATCTG GGAGTAAGAG AAAGCTTTAG CATGGCAAAC 
AACATAATTC TTTACTGTGA CACAAATTCA GACTCCCTGC AGTCACTGAA GATGTGCACA 
GGTAATTACA CATTTATTCC TTACATGATC ACGCCACAAA ACAAAGTGTT TTGCTGTGAG 
AGTATTTTCA TGAGAGGAAT GACTGAACTA ATGCCGTCAA ACTTTGAGTT ACTTCTTGGT 
CCGATCTGCC TGCCCCTAGT GGATCGCTTT GTTCAGATTC TGAAGGTGGC ACAGGCCACG 
TCATGCCAAT ATTTTCGAGA GGCAATACTA AATGATATTA GAAGGGCTCG GAACCAATAC 
ACTGGGAAGG ATCTGGCTGC TGAGCTAATA AGGATCCGCC AGCGCGTAGA CAGCATCGAG 
TTGTTATCAC CTGACATCAT TATCAATCTG CTTTTGTCAT ACAGAGATAT CCAGGACTAC 
GATTCAGTTG TGACGTTGGT GGAAATATTG GAGAAACTTC CAACCTTTAA TTTATCTTCC 
AATCACCATA TAAAATTTCA TTATGCATTT GCATTAAACA GGAGAAATCA TCCAGGTGAC 
AGAGTAAAAG CCCTTGAAAT AATGTTGCCT TTGGTGCAAC TTGAAGATCA AGTAGCTTCA 
GACATATACT GCATTGTGGG AAGGATCTAC AAAGATATGT TCTTAGATTC TAACTTCATG 
GATACCGAAA GTCGTGATAA TGCTATACAT TGGTACAGGA AGGCCTTTGA ATCTGAACCA 
ACACTTCATT CTGGAATCAA TTATGCGGTG CTACTTCTAG CTGCTGGTCA TCAATTTGAG 
ACTTCTTTGG AACTCCGGAA AATCGGAGTT AAACTCAGTA GCCTGTTGGG TAAAAAAGGA 
AATCTCGAAA AACTCCAAAG TTATTGGGAT GTTGGATTTT ATTTGGGGGC ATGTATACTG 
GCCAATGACT CATCAAAAGT TATCCATGCA GCAGAAAAGC TTTTCAAGCT CAAAACCCCA 
GCATGGTTCT TGAATTCAGT TGTGGAGACC ATTTTGATTT ATCAGCATTT TAAAACGCCA 
AACCAAGAAC AACATGTAAC AAACCATGAG CTGGTTGATT TTTGGATGGA TTTTCTTGTT 
GAGTCTACAA GTAGCAATGT GTCTGTATTA CGGTTTCCTG TCTTAATACT AGAGCCAACA 
AAAATCTACC AGCCTTCCTA CCTATCAATC AATAGTGAAG CTGAAGTTCA GACAATATCT 
ATTTGGCACG TTCTACCTGG CGATAAGAAA GGAATCCATG AATGGAATTT CCCTGCAGAT 
TCTATCAGAG GTGTAAGCAT TTCAAAGTTT GAAGAAAGGT GCTGTTTTCT GTATGTTCTG 
AACAACTCTG ACGATTTTCA AATATATTTC TGCACAGAGC TCCACTGCAA AAAATTCTTT 
GATAAAGTAA ACCGTATCAA AGAAGAGAAT GGAAAGAACA CAGAAGAAGG GGAGTGTGAT 
GGAGATTTAA TGGAGTTCGA CTATGAGTAT GATGAAAATG GGGACAGAGT GGTTCTCGGT 
AAAGGGAAAC ATGGAGTGGT ATATGCAGGA AGAGACTTGA GTAACCAGGT CAGAATTGCA 
ATTAAAGAAG TTCCAGAGAG AGATGGTCGA TGCTCTCAAC CACTTCATGA AGAGATTGCT 
TTGCATAAAC GTCTAAAGCA TAAAAACATT GTTCAGTATT TGGGATCATT CAGTAAAGGT 
GGTTTCATTA AAATATTTAT GGAACAAGTA CCTGGAGGAA GCCTCTCCGC ACTTCTAAGG 
TCAAAATGGG GACCCTTGAA AGACAATGAG CAGACAATCG GATTTTATAC CAAACAGATC 
CTTGAAGGTT TGAAATATCT CCATGATAAC CAGATAGTTC ATAGAGATAT CAAGGGTGAT 
AATGTTCTTA TAAACACATA CAGTGGTGTT CTGAAAATCT CTGATTTTGG CACTTCAAAG 
AGACTTGCTG GAATCAACCC AAATACAGAA ACCTTCACTG GCACTCTACA ATATATGGCA 
CCAGAAATTA TAGACAAAGG ACCAAGAGGA TATGGAAAGC CAGCAGACAT TTGGTCTCTG 
GGATGCACAG TTATAGAAAT GGCAACAGGA AAACCACCAT TTTATGAGCT TGGAGAACCA 
CAAGCTGCCA TGTTTAAAGT GGGCATGTTT AAAATTCATC CAGATATCCC AGAATCTATG 
TCGTCTGAAG CTAAGGCATT TTTGTTGCGG TGCTTTGAAC CAGATCCTGT GAAACGCGCT 
ACTGCTACTG AACTTATAAC GGATGAATTC TTGAAGGTGT CCTCTAGGAA ACGCAAGTCC 
CAGCTTAAGC TTACAACTCT TTCCTCTGGC TCAAATGAAT ATCTCCGTAG CATATCTTTG 
CCGGTGTCAG TGATGGTGGA AGATACAAGC AGCAGCAGTG AATACAGTTC CGTTTCTCCA 
GATTCAGACT TAAGATCGGA CCCATTTACT TTTAAAACAA GAGCCAGATC ATGTATAGAA 
AAGGATGTCA AGCTTAACAG AAGTTTATTT CTAGGAATTC CAGACGAGAA TTTTGAAGAC 
AGTAGTGCTC CTCCTTCTCC TGAAGAACGA GACTCTGGAT TTTTCATGCT GAAGAAAGAT 
AGTGAAAGGA GGGCTACACT TTATCATATT CTTACAGAGG ATCAGGACAA GGTGGCTAGA 
AACTTAATGG ATGCTTTAGT CCAGGGATCA GAAATAAAAT TGAAATGGGA CCACATCACT 
GCTATTGTTG ACAGCCTAAA AGAGTTTGTA AGATCTACTG ACAGGAAAAT CATTTCAACA 
ACACTATCAA AACTCAAACT TGAGTTAGAC TTTGATAGTC ATGCTATCAG TCAAGTCCAG 
ATGGTGCTGT TTGGCTTTCA AGATGCGGTA AATAAAGTGC TACGAAATCA TAACATAAAG 
CCTCACTGGA TGTTTGCCTT AGATAATATA ATTCGAAAGG CCGTGCAGAC AGCTATCACT 
ATCCTGGTAC CTGAGCTGAG GCCACATTTT AGCATTACAT CAGAAAGTGA CCAAGCAGAT 
CAGGAGGACA TAGAAGTAGA GGAAAGAAGT AATAAACAAC CCACAGAACA AACCATTCAC 
AGAGAAGTCA ATGAGGACAA CGTGCAAACC TCAGGAGTGA GCACACTCAG TTCAACTTTG 
TCCCACAACT CTCAGAATGC CGACAGGTCC CTGAATGTGC AACTTGAAAG ACTTAGGTTA 
GAGTCAAACA GGCTTTTGGA AGAATTGTTA CTTAAAGAGA GAGAATTCCA AGTCCTTCTA 
CGCCAAGCTA TTGAAGTGAA GGACAGGGAA ATCAAACGCC TGCAGTGCAC GTCTGAGCCA 
ATAAGCACTT CCGTATCCAG CACAAGGTCA ACTATCTCAG GCAGTGAAGA CTTGAATCTT 
GTTTACTGGC TACACAAGTG CAGAGTAGAT GAAGATACAA TAAGCAAGTT TTTGGCAGAA 
GACTATCAGC TTGAAGATAT TCTTTGTCAT GTCACACGAG ATGATTTAAA ATCTCTGAAT 
CTGAGGGGAG GAGTACTATG CACAATATGG AAAGAAATCA CTGACTACAG AGAAAAACAA 
GCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	map3k5
Entrez Gene ID	100497692
Full Name	mitogen-activated protein kinase kinase kinase 5
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Pan troglodytes (chimpanzee)	MAP3K5	XP_001171211.1
	MAP3K5	XP_002803972.1
Mus musculus (house mouse)	Map3k5	NP_032606.4
Danio rerio (zebrafish)	map3k5	NP_001155222.1
Homo sapiens (human)	MAP3K5	NP_005914.1
Rattus norvegicus (Norway rat)	Map3k5	NP_001264623.1
Gallus gallus (chicken)	MAP3K5	XP_419725.3
Xenopus tropicalis (tropical clawed frog)	LOC100497692	XP_002936311.2
Canis lupus familiaris (dog)	MAP3K5	XP_533420.4
Bos taurus (cattle)	MAP3K5	NP_001137553.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

map3k5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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