dot1l cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	dot1l
Entrez Gene ID	100497594
Full Name	DOT1-like histone H3K79 methyltransferase
General protein information	Preferred Names DOT1-like histone H3K79 methyltransferase Names histone-lysine N-methyltransferase, H3 lysine-79 specific
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012954295.2	XP_012809749.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
XM_018096995.1	XP_017952484.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X2
XM_018096997.1	XP_017952486.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X3
XM_018096998.1	XP_017952487.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X4
XM_012954295.3	XP_012809749.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
XM_018096998.2	XP_017952487.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DOT1L	XP_003953365.1
Canis lupus familiaris (dog)	DOT1L	XP_005633296.1
Homo sapiens (human)	DOT1L	NP_115871.1
	DOT1L	XP_001097213.2
Xenopus tropicalis (tropical clawed frog)	dot1l	XP_002937984.2
Bos taurus (cattle)	DOT1L	XP_005209126.1
Mus musculus (house mouse)	Dot1l	NP_955354.1
Rattus norvegicus (Norway rat)	Dot1l	NP_001102203.1
Gallus gallus (chicken)	DOT1L	XP_004948823.1
Danio rerio (zebrafish)	dot1l	XP_005170590.1

Gene Ontology

The following dot1l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dot1l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa42027	XM_018096995.1 Latest version!	Xenopus tropicalis DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OXa42028	XM_018096997.1 Latest version!	Xenopus tropicalis DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OXa42029	XM_018096998.1 Latest version!	Xenopus tropicalis DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OXa48512	XM_012954295.3 Latest version!	Xenopus tropicalis DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa48513	XM_018096998.2 Latest version!	Xenopus tropicalis DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa36930	XM_012954295.2 Latest version!	Xenopus tropicalis DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dot1l ( XM_018096995.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952484.1 Xenopus tropicalis DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa42027
Clone ID Related Accession (Same CDS sequence)	XM_018096995.1
Accession Version	XM_018096995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGAGA AGCTGGAACT AAGGCTGAAG TCCCCAGTGG GAGCCGAGCC TGCCGTCTAC 
CCTTGGCCGC TGCCTGTTTA CGATAAACAC CATGATGCTG CACATGAAAT TATTGAAACC 
ATCAGCTTTG AGAGCATGCA GCGGCTGTGT GAGAAGTATA ACAGAGCCAT TGACAGCATC 
CATCAGCTGT GGAAAGGCAC CACTCATCCT ATGAAGTTGA AAACTCGCCC TTCTACTGGT 
CTCCTTCGCC ACATTTTGCA GCAGGTCTAC AACCACTCTG TAACAGACCC TGAAAAACTC 
AACAATTATG AGCCGTTTTC TCCTGAGGTC TATGGAGAAA CCTCCTTTGA TTTGGTTGCC 
CAGATGATTG ATGAAATTAA GATGACTGAG GATGATCTCT TTGTGGACCT GGGAAGTGGT 
GTCGGGCAGG TTGTTCTGCA GGTTGCTGCA GCAACAGGTT GCAAGCATCA TTATGGTGTA 
GAGAAAGCGG ATATCCCAGC CAAGTATGCA GAGACAATGG ACACTGAGTT CAGGAAATGG 
ATGAAGTGGT ATGGAAAGAA GCATTCAGAG TACACACTGG AGCGTGGAGA CTTCCTCTCA 
GAGGAGTGGA GGGAAAGAAT AGCTAACACA AGTGTTATTT TTGTGAATAA TTTTGCCTTT 
GGTCCTGAGG TTGACCACCA GCTAAAGGAG AGATTCGCCA ACATGAAGGA AGGTGGCAGA 
ATCGTATCCT CCAAACCATT TGCACCTCTG AATTTCAGAA TAAATAGCCG AAACTTGAGT 
GATATTGGCA CTATTATGAG AGTTGTGGAG TTATCACCTC TTAAAGGATC TGTATCTTGG 
ACTGGGAAGC CTGTTTCTTA TTACCTGCAC ACTATAGACC GGACCTTACT TGAAAATTAT 
TTTTCTAGTT TGAAAAATCC AAAACTCAGG GAGGAACAGG AAGCAGCAAG ACGCAGACAA 
CAGAGAGAAG GGAAGAGCAA TACCACAACT CCTACAAAAG TTCATGAGAC AAAGGACTCT 
GCTGCAGAGG AAGAAAAGTC TGCCCCAGTG AAAAAGGCTT CTCCAGCCAA AGCCCGAAGA 
AAGCTCAGCA AAAAGGGCAG AAAAATGGCA GGTCGCAAGC GGGGACGTCC AAAAAAGTTG 
CTTACCACAA CAGCTGACCG CAAGTCCTCT AAATCACAGA CTGCACTGGA AACCCTCCAT 
CCACAAAATG TCCTTGTTCC TCAGTCTCCA ACAAAATCAC CCCCTCAGGA TTCATACAAA 
ACACCTCATA GTCCCTATTA TCAGCTACCT CCAAAAGTGC AACGTCAGCC TTCCAATCAG 
CTTCTGTCAT CTCCTACCCC TCCAGCCCTA CAGAAATTGC TCGATTCATT TAGGATCCAG 
TATTTGCAAC TTATGTCTTA CATGAAAACA CCCCATTACA AGGCTGGTCT GCAGCAACTG 
CTGGAACAGG AAAAGGAAAA GAATTCCCAC CTTCTTGGTG CTGCTCAGCA GCTGCTCACC 
CACTGTCAGG CTCAGAAAGA AGAAATAAAG AAACTTTTTC AGCAGAAACT GGATGAGCTG 
GGTGTTAAGG CCCTCACTTA CAGTGACCTC ATTCAAGCGC AGAAAGAGAT TTTTGCTCAC 
AACCAGCAGC TGAAAGAGCA AAGTCGACAG TTAGAGGAAG AGAATGGAGC ATTAAGAAAC 
CACAGCTTGC AGCTGCTGAA GGCACGTTGT GAAGAATTAA AGCTGGACTG GAATACACTG 
TCTTTGGAAA ATCTCATGAA GGAGAAGTTG GCCCTAAAAA ATCAGATATC CGAAAAGCAG 
AAGCACTGTT TGGAGCTACA AATCAGCATT GTGGAACTAG AAAAGTTTCA GAGACAACAA 
GAGCTGCTCC AGCTCAAGTC TTCCACCTTG CCAGAGGAAC CACCACCCAG CCATAATCGA 
ACCAAAGGTA GCCTTGGCAA CCACTTCCAC GCTGAACAGT CTTCAGTGCG CATGATGCCT 
CCTCCATCTT CTCACACAAA TGGTCTAAGC CCTGAGCGGT CCTTGAATGG TCATGCTTCT 
CATTATGAGC TGTACCATTC CTGTCGAGGA GGAAGCAGGC CCTGCTCCAA GGAAAGTACA 
CCATCTCACT CAGAGCATGC CGACATTGTG CCCAGTACCC AAACCCATGC AGCCAAGCAC 
AAAGGAGAGA AAATATCTGG CCCAACCCAA CCAGACTACA CGCGCTACTC CCCTGCCAAA 
ATTGCAATGA GACGGCACCT GAACCAGGAA CCCTTACCCA ATGGTAGAAG CACTGAAATA 
AATCATAGGT GTAATGTTAC AAAAGACCAC AGCTTCCCAT CCATAAGCCC TGGAATTCCA 
AATGGTGTCC AATTTAGCCC TCAGGAATCA AATCCTTCTT CCCCTGAGTT AACTCATACA 
CCAGGGGAGA GAGGACCTGA AAAGATGTTT CGAGAGCGAA ATTCCCCTTC TAATCCAGAA 
ACCATCACCA GTCTGCCTAT TAGTATTCCC CTGAGCACAG TCCAGCCTAA CAAGCTGCCA 
GTCAGTATTC CACTGGCCAG TGTAGTCCTG CCAAGTCGTG TGGAAAAGAG AAACAGCCCC 
AGTCCCGGAT GTCACAGCAG AGATGGCTCA CTTTCAGAGA ATCAATATGG TGCTACTTCT 
AATAGCCTCA GTTATGCTGC TGTCTCAAAA CCATTGGCTA CCTCAGGTTT CTTCTATCCA 
ACCTGGTCTT CATCTCTAAA TGGACCAGTT TTGAATAGCC ATGGGTCAGA GACGGATGGA 
ATGGATGACT CATCAAATTC TGGCACATTG TCTAATTCAG GAGGATCACG GAGCTCCACC 
CCTTCCCACA GTCACCTGAT CTCTCAGCGC TCCCCTGCCC AACATTCCAC ATCTGCTAGT 
CCTTGCACAT TTCCAGATCC AAGCAAAGGT TCTGGTGAAG GGGATATTGA CACAAGTGAT 
GCTGAAATGG AAGCAAAGCG TCGGATTATC TTCACCATTA CCAGTGGAAG CTCCAACGGC 
AGGTCACCAT CAAACAAACA CAGCCCTTTG ACTGCCACAA TCCGCATGGA GTGTGGCACA 
GGGCAGGATG CCAAAAGGAG AAGTGGTAGA CGAAAAAGAT CCTCTGCTGG CAGTCACAAT 
TCAGCTGTAT CACCCAAACG CAGATCTCTT CCCACATCTG TTGGTCTAAA CAGTTATACT 
TCAGGATCTC CACTGAATAT CAGCTCAATG GTGAGTAACA TTAACCAGCC CTTGGAGATT 
ACTGCAATCT CTTCCCCTGA AGCCTCTATG AAGAGTTCCC CTGCTTCCTA TCAGGATCAT 
GAGTTGCCCC CAGTTTTAAA AAAGGAAAAA CCCCTTTCTT TATCTAATGG CGTTCATTAT 
TCACCCTTAA CAACAGAGGA TGAGGCTGAG TCCGAGGAAG ACACCCAGAG TACAAGAACG 
GAGCGAAAGA GTTCCAGCCT GTGCTTTGAA AGCAAAGACT CCCCCCAGAG AAGTGAAGGG 
GAAAGAGGTC TCCCATACTC TAGCAAGAAG TCTTCATGTG AGAGTGTAAA ATGGAAAGCA 
ACATTCTCTC CAATTTCTGA TGCAACTCCG GGTCGTGCCT CTGACAGCCC TTCCTCACCT 
CACCGTCAGA ACACACATTA TTCATCTGGA GAGGGAGGCC GCAGAGGATC AGGGGGATTG 
AGTCCTGGCT CTGCAAATGG CCACAGTGAC CATCCTAGGG TAGAAGGGGG CAAAGGAGAG 
TGCAACCTTT TCTTAAGTAA AAAGATGTCT TCTGAAGGTA GCAAAAGAGA AGACAGGGGT 
CCTACAGCAA AACTGGATCG TTCCAGCAGT AAAGTCCCTC GAAGCCGTGA AAGGGATGGG 
AACACTGTGT TCATGACACA AGGAACTGGT GCTCTCATGA ATGGCAAGGG ACCTGGCTCA 
AATCCTTTAC ACCCACACCA TCATTTTTTG AACAGCTTGG CAGCTGGCTC TCAGCTTGCC 
TTGCAAGGTG GGCTTAACTC TGTTGCTAGC AATTCTGTTC TGCAGTCCAT CTTCAGCTCC 
ATCCCTGTAC ATGTGACTGG GCCAGCTGGA CGATTGTCTA ACTCTCACAG CATGTCTGGT 
TTCACAGCTC CTGGAGGTAC TGTAGGAGGT ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952484.1
CDS	168..4283
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETISFESMQRLCEKYNRAIDSIHQLWKGTTHPMKLKTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATGCKHHYGVEKADIPAKYAETMDTEFRKWMKWYGKKHSEYTLERGDFLSEEWRERIANTSVIFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTLLENYFSSLKNPKLREEQEAARRRQQREGKSNTTTPTKVHETKDSAAEEEKSAPVKKASPAKARRKLSKKGRKMAGRKRGRPKKLLTTTADRKSSKSQTALETLHPQNVLVPQSPTKSPPQDSYKTPHSPYYQLPPKVQRQPSNQLLSSPTPPALQKLLDSFRIQYLQLMSYMKTPHYKAGLQQLLEQEKEKNSHLLGAAQQLLTHCQAQKEEIKKLFQQKLDELGVKALTYSDLIQAQKEIFAHNQQLKEQSRQLEEENGALRNHSLQLLKARCEELKLDWNTLSLENLMKEKLALKNQISEKQKHCLELQISIVELEKFQRQQELLQLKSSTLPEEPPPSHNRTKGSLGNHFHAEQSSVRMMPPPSSHTNGLSPERSLNGHASHYELYHSCRGGSRPCSKESTPSHSEHADIVPSTQTHAAKHKGEKISGPTQPDYTRYSPAKIAMRRHLNQEPLPNGRSTEINHRCNVTKDHSFPSISPGIPNGVQFSPQESNPSSPELTHTPGERGPEKMFRERNSPSNPETITSLPISIPLSTVQPNKLPVSIPLASVVLPSRVEKRNSPSPGCHSRDGSLSENQYGATSNSLSYAAVSKPLATSGFFYPTWSSSLNGPVLNSHGSETDGMDDSSNSGTLSNSGGSRSSTPSHSHLISQRSPAQHSTSASPCTFPDPSKGSGEGDIDTSDAEMEAKRRIIFTITSGSSNGRSPSNKHSPLTATIRMECGTGQDAKRRSGRRKRSSAGSHNSAVSPKRRSLPTSVGLNSYTSGSPLNISSMVSNINQPLEITAISSPEASMKSSPASYQDHELPPVLKKEKPLSLSNGVHYSPLTTEDEAESEEDTQSTRTERKSSSLCFESKDSPQRSEGERGLPYSSKKSSCESVKWKATFSPISDATPGRASDSPSSPHRQNTHYSSGEGGRRGSGGLSPGSANGHSDHPRVEGGKGECNLFLSKKMSSEGSKREDRGPTAKLDRSSSKVPRSRERDGNTVFMTQGTGALMNGKGPGSNPLHPHHHFLNSLAAGSQLALQGGLNSVASNSVLQSIFSSIPVHVTGPAGRLSNSHSMSGFTAPGGTVGGI

Target ORF information:

RefSeq Version	XM_018096995.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096995.1

ORF Insert Sequence:

ATGGGCGAGA AGCTGGAACT AAGGCTGAAG TCCCCAGTGG GAGCCGAGCC TGCCGTCTAC 
CCTTGGCCGC TGCCTGTTTA CGATAAACAC CATGATGCTG CACATGAAAT TATTGAAACC 
ATCAGCTTTG AGAGCATGCA GCGGCTGTGT GAGAAGTATA ACAGAGCCAT TGACAGCATC 
CATCAGCTGT GGAAAGGCAC CACTCATCCT ATGAAGTTGA AAACTCGCCC TTCTACTGGT 
CTCCTTCGCC ACATTTTGCA GCAGGTCTAC AACCACTCTG TAACAGACCC TGAAAAACTC 
AACAATTATG AGCCGTTTTC TCCTGAGGTC TATGGAGAAA CCTCCTTTGA TTTGGTTGCC 
CAGATGATTG ATGAAATTAA GATGACTGAG GATGATCTCT TTGTGGACCT GGGAAGTGGT 
GTCGGGCAGG TTGTTCTGCA GGTTGCTGCA GCAACAGGTT GCAAGCATCA TTATGGTGTA 
GAGAAAGCGG ATATCCCAGC CAAGTATGCA GAGACAATGG ACACTGAGTT CAGGAAATGG 
ATGAAGTGGT ATGGAAAGAA GCATTCAGAG TACACACTGG AGCGTGGAGA CTTCCTCTCA 
GAGGAGTGGA GGGAAAGAAT AGCTAACACA AGTGTTATTT TTGTGAATAA TTTTGCCTTT 
GGTCCTGAGG TTGACCACCA GCTAAAGGAG AGATTCGCCA ACATGAAGGA AGGTGGCAGA 
ATCGTATCCT CCAAACCATT TGCACCTCTG AATTTCAGAA TAAATAGCCG AAACTTGAGT 
GATATTGGCA CTATTATGAG AGTTGTGGAG TTATCACCTC TTAAAGGATC TGTATCTTGG 
ACTGGGAAGC CTGTTTCTTA TTACCTGCAC ACTATAGACC GGACCTTACT TGAAAATTAT 
TTTTCTAGTT TGAAAAATCC AAAACTCAGG GAGGAACAGG AAGCAGCAAG ACGCAGACAA 
CAGAGAGAAG GGAAGAGCAA TACCACAACT CCTACAAAAG TTCATGAGAC AAAGGACTCT 
GCTGCAGAGG AAGAAAAGTC TGCCCCAGTG AAAAAGGCTT CTCCAGCCAA AGCCCGAAGA 
AAGCTCAGCA AAAAGGGCAG AAAAATGGCA GGTCGCAAGC GGGGACGTCC AAAAAAGTTG 
CTTACCACAA CAGCTGACCG CAAGTCCTCT AAATCACAGA CTGCACTGGA AACCCTCCAT 
CCACAAAATG TCCTTGTTCC TCAGTCTCCA ACAAAATCAC CCCCTCAGGA TTCATACAAA 
ACACCTCATA GTCCCTATTA TCAGCTACCT CCAAAAGTGC AACGTCAGCC TTCCAATCAG 
CTTCTGTCAT CTCCTACCCC TCCAGCCCTA CAGAAATTGC TCGATTCATT TAGGATCCAG 
TATTTGCAAC TTATGTCTTA CATGAAAACA CCCCATTACA AGGCTGGTCT GCAGCAACTG 
CTGGAACAGG AAAAGGAAAA GAATTCCCAC CTTCTTGGTG CTGCTCAGCA GCTGCTCACC 
CACTGTCAGG CTCAGAAAGA AGAAATAAAG AAACTTTTTC AGCAGAAACT GGATGAGCTG 
GGTGTTAAGG CCCTCACTTA CAGTGACCTC ATTCAAGCGC AGAAAGAGAT TTTTGCTCAC 
AACCAGCAGC TGAAAGAGCA AAGTCGACAG TTAGAGGAAG AGAATGGAGC ATTAAGAAAC 
CACAGCTTGC AGCTGCTGAA GGCACGTTGT GAAGAATTAA AGCTGGACTG GAATACACTG 
TCTTTGGAAA ATCTCATGAA GGAGAAGTTG GCCCTAAAAA ATCAGATATC CGAAAAGCAG 
AAGCACTGTT TGGAGCTACA AATCAGCATT GTGGAACTAG AAAAGTTTCA GAGACAACAA 
GAGCTGCTCC AGCTCAAGTC TTCCACCTTG CCAGAGGAAC CACCACCCAG CCATAATCGA 
ACCAAAGGTA GCCTTGGCAA CCACTTCCAC GCTGAACAGT CTTCAGTGCG CATGATGCCT 
CCTCCATCTT CTCACACAAA TGGTCTAAGC CCTGAGCGGT CCTTGAATGG TCATGCTTCT 
CATTATGAGC TGTACCATTC CTGTCGAGGA GGAAGCAGGC CCTGCTCCAA GGAAAGTACA 
CCATCTCACT CAGAGCATGC CGACATTGTG CCCAGTACCC AAACCCATGC AGCCAAGCAC 
AAAGGAGAGA AAATATCTGG CCCAACCCAA CCAGACTACA CGCGCTACTC CCCTGCCAAA 
ATTGCAATGA GACGGCACCT GAACCAGGAA CCCTTACCCA ATGGTAGAAG CACTGAAATA 
AATCATAGGT GTAATGTTAC AAAAGACCAC AGCTTCCCAT CCATAAGCCC TGGAATTCCA 
AATGGTGTCC AATTTAGCCC TCAGGAATCA AATCCTTCTT CCCCTGAGTT AACTCATACA 
CCAGGGGAGA GAGGACCTGA AAAGATGTTT CGAGAGCGAA ATTCCCCTTC TAATCCAGAA 
ACCATCACCA GTCTGCCTAT TAGTATTCCC CTGAGCACAG TCCAGCCTAA CAAGCTGCCA 
GTCAGTATTC CACTGGCCAG TGTAGTCCTG CCAAGTCGTG TGGAAAAGAG AAACAGCCCC 
AGTCCCGGAT GTCACAGCAG AGATGGCTCA CTTTCAGAGA ATCAATATGG TGCTACTTCT 
AATAGCCTCA GTTATGCTGC TGTCTCAAAA CCATTGGCTA CCTCAGGTTT CTTCTATCCA 
ACCTGGTCTT CATCTCTAAA TGGACCAGTT TTGAATAGCC ATGGGTCAGA GACGGATGGA 
ATGGATGACT CATCAAATTC TGGCACATTG TCTAATTCAG GAGGATCACG GAGCTCCACC 
CCTTCCCACA GTCACCTGAT CTCTCAGCGC TCCCCTGCCC AACATTCCAC ATCTGCTAGT 
CCTTGCACAT TTCCAGATCC AAGCAAAGGT TCTGGTGAAG GGGATATTGA CACAAGTGAT 
GCTGAAATGG AAGCAAAGCG TCGGATTATC TTCACCATTA CCAGTGGAAG CTCCAACGGC 
AGGTCACCAT CAAACAAACA CAGCCCTTTG ACTGCCACAA TCCGCATGGA GTGTGGCACA 
GGGCAGGATG CCAAAAGGAG AAGTGGTAGA CGAAAAAGAT CCTCTGCTGG CAGTCACAAT 
TCAGCTGTAT CACCCAAACG CAGATCTCTT CCCACATCTG TTGGTCTAAA CAGTTATACT 
TCAGGATCTC CACTGAATAT CAGCTCAATG GTGAGTAACA TTAACCAGCC CTTGGAGATT 
ACTGCAATCT CTTCCCCTGA AGCCTCTATG AAGAGTTCCC CTGCTTCCTA TCAGGATCAT 
GAGTTGCCCC CAGTTTTAAA AAAGGAAAAA CCCCTTTCTT TATCTAATGG CGTTCATTAT 
TCACCCTTAA CAACAGAGGA TGAGGCTGAG TCCGAGGAAG ACACCCAGAG TACAAGAACG 
GAGCGAAAGA GTTCCAGCCT GTGCTTTGAA AGCAAAGACT CCCCCCAGAG AAGTGAAGGG 
GAAAGAGGTC TCCCATACTC TAGCAAGAAG TCTTCATGTG AGAGTGTAAA ATGGAAAGCA 
ACATTCTCTC CAATTTCTGA TGCAACTCCG GGTCGTGCCT CTGACAGCCC TTCCTCACCT 
CACCGTCAGA ACACACATTA TTCATCTGGA GAGGGAGGCC GCAGAGGATC AGGGGGATTG 
AGTCCTGGCT CTGCAAATGG CCACAGTGAC CATCCTAGGG TAGAAGGGGG CAAAGGAGAG 
TGCAACCTTT TCTTAAGTAA AAAGATGTCT TCTGAAGGTA GCAAAAGAGA AGACAGGGGT 
CCTACAGCAA AACTGGATCG TTCCAGCAGT AAAGTCCCTC GAAGCCGTGA AAGGGATGGG 
AACACTGTGT TCATGACACA AGGAACTGGT GCTCTCATGA ATGGCAAGGG ACCTGGCTCA 
AATCCTTTAC ACCCACACCA TCATTTTTTG AACAGCTTGG CAGCTGGCTC TCAGCTTGCC 
TTGCAAGGTG GGCTTAACTC TGTTGCTAGC AATTCTGTTC TGCAGTCCAT CTTCAGCTCC 
ATCCCTGTAC ATGTGACTGG GCCAGCTGGA CGATTGTCTA ACTCTCACAG CATGTCTGGT 
TTCACAGCTC CTGGAGGTAC TGTAGGAGGT ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dot1l ( XM_018096997.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952486.1 Xenopus tropicalis DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa42028
Clone ID Related Accession (Same CDS sequence)	XM_018096997.1
Accession Version	XM_018096997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGAGA AGCTGGAACT AAGGCTGAAG TCCCCAGTGG GAGCCGAGCC TGCCGTCTAC 
CCTTGGCCGC TGCCTGTTTA CGATAAACAC CATGATGCTG CACATGAAAT TATTGAAACC 
ATCAGATGGG TCTGTGAAGA AATTCCAGAT CTCAAGCTTG CAATGGAAAA CTATGTTCTG 
ATAGACTATG ACACAAAGTG CTTTGAGAGC ATGCAGCGGC TGTGTGAGAA GTATAACAGA 
GCCATTGACA GCATCCATCA GCTGTGGAAA GGCACCACTC ATCCTATGAA GTTGAAAACT 
CGCCCTTCTA CTGGTCTCCT TCGCCACATT TTGCAGCAGG TCTACAACCA CTCTGTAACA 
GACCCTGAAA AACTCAACAA TTATGAGCCG TTTTCTCCTG AGGTCTATGG AGAAACCTCC 
TTTGATTTGG TTGCCCAGAT GATTGATGAA ATTAAGATGA CTGAGGATGA TCTCTTTGTG 
GACCTGGGAA GTGGTGTCGG GCAGGTTGTT CTGCAGGTTG CTGCAGCAAC AGGTTGCAAG 
CATCATTATG GTGTAGAGAA AGCGGATATC CCAGCCAAGT ATGCAGAGAC AATGGACACT 
GAGTTCAGGA AATGGATGAA GTGGTATGGA AAGAAGCATT CAGAGTACAC ACTGGAGCGT 
GGAGACTTCC TCTCAGAGGA GTGGAGGGAA AGAATAGCTA ACACAAGTGT TATTTTTGTG 
AATAATTTTG CCTTTGGTCC TGAGGTTGAC CACCAGCTAA AGGAGAGATT CGCCAACATG 
AAGGAAGGTG GCAGAATCGT ATCCTCCAAA CCATTTGCAC CTCTGAATTT CAGAATAAAT 
AGCCGAAACT TGAGTGATAT TGGCACTATT ATGAGAGTTG TGGAGTTATC ACCTCTTAAA 
GGATCTGTAT CTTGGACTGG GAAGCCTGTT TCTTATTACC TGCACACTAT AGACCGGACC 
TTACTTGAAA ATTATTTTTC TAGTTTGAAA AATCCAAAAC TCAGGGAGGA ACAGGAAGCA 
GCAAGACGCA GACAACAGAG AGAAGGGAAG AGCAATACCA CAACTCCTAC AAAAGTTCAT 
GAGACAAAGG ACTCTGCTGC AGAGGAAGAA AAGTCTGCCC CAGTGAAAAA GGCTTCTCCA 
GCCAAAGCCC GAAGAAAGCT CAGCAAAAAG GGCAGAAAAA TGGCAGGTCG CAAGCGGGGA 
CGTCCAAAAA AGTTGCTTAC CACAACAGCT GACCGCAAGT CCTCTAAATC ACAGACTGCA 
CTGGAAACCC TCCATCCACA AAATGTCCTT GTTCCTCAGT CTCCAACAAA ATCACCCCCT 
CAGGATTCAT ACAAAACACC TCATAGTCCC TATTATCAGC TACCTCCAAA AGTGCAACGT 
CAGCCTTCCA ATCAGCTTCT GTCATCTCCT ACCCCTCCAG CCCTACAGAA ATTGCTCGAT 
TCATTTAGGA TCCAGTATTT GCAACTTATG TCTTACATGA AAACACCCCA TTACAAGGCT 
GGTCTGCAGC AACTGCTGGA ACAGGAAAAG CTGGGTGTTA AGGCCCTCAC TTACAGTGAC 
CTCATTCAAG CGCAGAAAGA GATTTTTGCT CACAACCAGC AGCTGAAAGA GCAAAGTCGA 
CAGTTAGAGG AAGAGAATGG AGCATTAAGA AACCACAGCT TGCAGCTGCT GAAGGCACGT 
TGTGAAGAAT TAAAGCTGGA CTGGAATACA CTGTCTTTGG AAAATCTCAT GAAGGAGAAG 
TTGGCCCTAA AAAATCAGAT ATCCGAAAAG CAGAAGCACT GTTTGGAGCT ACAAATCAGC 
ATTGTGGAAC TAGAAAAGTT TCAGAGACAA CAAGAGCTGC TCCAGCTCAA GTCTTCCACC 
TTGCCAGAGG AACCACCACC CAGCCATAAT CGAACCAAAG GTAGCCTTGG CAACCACTTC 
CACGCTGAAC AGTCTTCAGT GCGCATGATG CCTCCTCCAT CTTCTCACAC AAATGGTCTA 
AGCCCTGAGC GGTCCTTGAA TGGTCATGCT TCTCATTATG AGCTGTACCA TTCCTGTCGA 
GGAGGAAGCA GGCCCTGCTC CAAGGAAAGT ACACCATCTC ACTCAGAGCA TGCCGACATT 
GTGCCCAGTA CCCAAACCCA TGCAGCCAAG CACAAAGGAG AGAAAATATC TGGCCCAACC 
CAACCAGACT ACACGCGCTA CTCCCCTGCC AAAATTGCAA TGAGACGGCA CCTGAACCAG 
GAACCCTTAC CCAATGGTAG AAGCACTGAA ATAAATCATA GGTGTAATGT TACAAAAGAC 
CACAGCTTCC CATCCATAAG CCCTGGAATT CCAAATGGTG TCCAATTTAG CCCTCAGGAA 
TCAAATCCTT CTTCCCCTGA GTTAACTCAT ACACCAGGGG AGAGAGGACC TGAAAAGATG 
TTTCGAGAGC GAAATTCCCC TTCTAATCCA GAAACCATCA CCAGTCTGCC TATTAGTATT 
CCCCTGAGCA CAGTCCAGCC TAACAAGCTG CCAGTCAGTA TTCCACTGGC CAGTGTAGTC 
CTGCCAAGTC GTGTGGAAAA GAGAAACAGC CCCAGTCCCG GATGTCACAG CAGAGATGGC 
TCACTTTCAG AGAATCAATA TGGTGCTACT TCTAATAGCC TCAGTTATGC TGCTGTCTCA 
AAACCATTGG CTACCTCAGG TTTCTTCTAT CCAACCTGGT CTTCATCTCT AAATGGACCA 
GTTTTGAATA GCCATGGGTC AGAGACGGAT GGAATGGATG ACTCATCAAA TTCTGGCACA 
TTGTCTAATT CAGGAGGATC ACGGAGCTCC ACCCCTTCCC ACAGTCACCT GATCTCTCAG 
CGCTCCCCTG CCCAACATTC CACATCTGCT AGTCCTTGCA CATTTCCAGA TCCAAGCAAA 
GGTTCTGGTG AAGGGGATAT TGACACAAGT GATGCTGAAA TGGAAGCAAA GCGTCGGATT 
ATCTTCACCA TTACCAGTGG AAGCTCCAAC GGCAGGTCAC CATCAAACAA ACACAGCCCT 
TTGACTGCCA CAATCCGCAT GGAGTGTGGC ACAGGGCAGG ATGCCAAAAG GAGAAGTGGT 
AGACGAAAAA GATCCTCTGC TGGCAGTCAC AATTCAGCTG TATCACCCAA ACGCAGATCT 
CTTCCCACAT CTGTTGGTCT AAACAGTTAT ACTTCAGGAT CTCCACTGAA TATCAGCTCA 
ATGGTGAGTA ACATTAACCA GCCCTTGGAG ATTACTGCAA TCTCTTCCCC TGAAGCCTCT 
ATGAAGAGTT CCCCTGCTTC CTATCAGGAT CATGAGTTGC CCCCAGTTTT AAAAAAGGAA 
AAACCCCTTT CTTTATCTAA TGGCGTTCAT TATTCACCCT TAACAACAGA GGATGAGGCT 
GAGTCCGAGG AAGACACCCA GAGTACAAGA ACGGAGCGAA AGAGTTCCAG CCTGTGCTTT 
GAAAGCAAAG ACTCCCCCCA GAGAAGTGAA GGGGAAAGAG GTCTCCCATA CTCTAGCAAG 
AAGTCTTCAT GTGAGAGTGT AAAATGGAAA GCAACATTCT CTCCAATTTC TGATGCAACT 
CCGGGTCGTG CCTCTGACAG CCCTTCCTCA CCTCACCGTC AGAACACACA TTATTCATCT 
GGAGAGGGAG GCCGCAGAGG ATCAGGGGGA TTGAGTCCTG GCTCTGCAAA TGGCCACAGT 
GACCATCCTA GGGTAGAAGG GGGCAAAGGA GAGTGCAACC TTTTCTTAAG TAAAAAGATG 
TCTTCTGAAG GTAGCAAAAG AGAAGACAGG GGTCCTACAG CAAAACTGGA TCGTTCCAGC 
AGTAAAGTCC CTCGAAGCCG TGAAAGGGAT GGGAACACTG TGTTCATGAC ACAAGGAACT 
GGTGCTCTCA TGAATGGCAA GGGACCTGGC TCAAATCCTT TACACCCACA CCATCATTTT 
TTGAACAGCT TGGCAGCTGG CTCTCAGCTT GCCTTGCAAG GTGGGCTTAA CTCTGTTGCT 
AGCAATTCTG TTCTGCAGTC CATCTTCAGC TCCATCCCTG TACATGTGAC TGGGCCAGCT 
GGACGATTGT CTAACTCTCA CAGCATGTCT GGTTTCACAG CTCCTGGAGG TACTGTAGGA 
GGTATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952486.1
CDS	168..4256
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKCFESMQRLCEKYNRAIDSIHQLWKGTTHPMKLKTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATGCKHHYGVEKADIPAKYAETMDTEFRKWMKWYGKKHSEYTLERGDFLSEEWRERIANTSVIFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTLLENYFSSLKNPKLREEQEAARRRQQREGKSNTTTPTKVHETKDSAAEEEKSAPVKKASPAKARRKLSKKGRKMAGRKRGRPKKLLTTTADRKSSKSQTALETLHPQNVLVPQSPTKSPPQDSYKTPHSPYYQLPPKVQRQPSNQLLSSPTPPALQKLLDSFRIQYLQLMSYMKTPHYKAGLQQLLEQEKLGVKALTYSDLIQAQKEIFAHNQQLKEQSRQLEEENGALRNHSLQLLKARCEELKLDWNTLSLENLMKEKLALKNQISEKQKHCLELQISIVELEKFQRQQELLQLKSSTLPEEPPPSHNRTKGSLGNHFHAEQSSVRMMPPPSSHTNGLSPERSLNGHASHYELYHSCRGGSRPCSKESTPSHSEHADIVPSTQTHAAKHKGEKISGPTQPDYTRYSPAKIAMRRHLNQEPLPNGRSTEINHRCNVTKDHSFPSISPGIPNGVQFSPQESNPSSPELTHTPGERGPEKMFRERNSPSNPETITSLPISIPLSTVQPNKLPVSIPLASVVLPSRVEKRNSPSPGCHSRDGSLSENQYGATSNSLSYAAVSKPLATSGFFYPTWSSSLNGPVLNSHGSETDGMDDSSNSGTLSNSGGSRSSTPSHSHLISQRSPAQHSTSASPCTFPDPSKGSGEGDIDTSDAEMEAKRRIIFTITSGSSNGRSPSNKHSPLTATIRMECGTGQDAKRRSGRRKRSSAGSHNSAVSPKRRSLPTSVGLNSYTSGSPLNISSMVSNINQPLEITAISSPEASMKSSPASYQDHELPPVLKKEKPLSLSNGVHYSPLTTEDEAESEEDTQSTRTERKSSSLCFESKDSPQRSEGERGLPYSSKKSSCESVKWKATFSPISDATPGRASDSPSSPHRQNTHYSSGEGGRRGSGGLSPGSANGHSDHPRVEGGKGECNLFLSKKMSSEGSKREDRGPTAKLDRSSSKVPRSRERDGNTVFMTQGTGALMNGKGPGSNPLHPHHHFLNSLAAGSQLALQGGLNSVASNSVLQSIFSSIPVHVTGPAGRLSNSHSMSGFTAPGGTVGGI

Target ORF information:

RefSeq Version	XM_018096997.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096997.1

ORF Insert Sequence:

ATGGGCGAGA AGCTGGAACT AAGGCTGAAG TCCCCAGTGG GAGCCGAGCC TGCCGTCTAC 
CCTTGGCCGC TGCCTGTTTA CGATAAACAC CATGATGCTG CACATGAAAT TATTGAAACC 
ATCAGATGGG TCTGTGAAGA AATTCCAGAT CTCAAGCTTG CAATGGAAAA CTATGTTCTG 
ATAGACTATG ACACAAAGTG CTTTGAGAGC ATGCAGCGGC TGTGTGAGAA GTATAACAGA 
GCCATTGACA GCATCCATCA GCTGTGGAAA GGCACCACTC ATCCTATGAA GTTGAAAACT 
CGCCCTTCTA CTGGTCTCCT TCGCCACATT TTGCAGCAGG TCTACAACCA CTCTGTAACA 
GACCCTGAAA AACTCAACAA TTATGAGCCG TTTTCTCCTG AGGTCTATGG AGAAACCTCC 
TTTGATTTGG TTGCCCAGAT GATTGATGAA ATTAAGATGA CTGAGGATGA TCTCTTTGTG 
GACCTGGGAA GTGGTGTCGG GCAGGTTGTT CTGCAGGTTG CTGCAGCAAC AGGTTGCAAG 
CATCATTATG GTGTAGAGAA AGCGGATATC CCAGCCAAGT ATGCAGAGAC AATGGACACT 
GAGTTCAGGA AATGGATGAA GTGGTATGGA AAGAAGCATT CAGAGTACAC ACTGGAGCGT 
GGAGACTTCC TCTCAGAGGA GTGGAGGGAA AGAATAGCTA ACACAAGTGT TATTTTTGTG 
AATAATTTTG CCTTTGGTCC TGAGGTTGAC CACCAGCTAA AGGAGAGATT CGCCAACATG 
AAGGAAGGTG GCAGAATCGT ATCCTCCAAA CCATTTGCAC CTCTGAATTT CAGAATAAAT 
AGCCGAAACT TGAGTGATAT TGGCACTATT ATGAGAGTTG TGGAGTTATC ACCTCTTAAA 
GGATCTGTAT CTTGGACTGG GAAGCCTGTT TCTTATTACC TGCACACTAT AGACCGGACC 
TTACTTGAAA ATTATTTTTC TAGTTTGAAA AATCCAAAAC TCAGGGAGGA ACAGGAAGCA 
GCAAGACGCA GACAACAGAG AGAAGGGAAG AGCAATACCA CAACTCCTAC AAAAGTTCAT 
GAGACAAAGG ACTCTGCTGC AGAGGAAGAA AAGTCTGCCC CAGTGAAAAA GGCTTCTCCA 
GCCAAAGCCC GAAGAAAGCT CAGCAAAAAG GGCAGAAAAA TGGCAGGTCG CAAGCGGGGA 
CGTCCAAAAA AGTTGCTTAC CACAACAGCT GACCGCAAGT CCTCTAAATC ACAGACTGCA 
CTGGAAACCC TCCATCCACA AAATGTCCTT GTTCCTCAGT CTCCAACAAA ATCACCCCCT 
CAGGATTCAT ACAAAACACC TCATAGTCCC TATTATCAGC TACCTCCAAA AGTGCAACGT 
CAGCCTTCCA ATCAGCTTCT GTCATCTCCT ACCCCTCCAG CCCTACAGAA ATTGCTCGAT 
TCATTTAGGA TCCAGTATTT GCAACTTATG TCTTACATGA AAACACCCCA TTACAAGGCT 
GGTCTGCAGC AACTGCTGGA ACAGGAAAAG CTGGGTGTTA AGGCCCTCAC TTACAGTGAC 
CTCATTCAAG CGCAGAAAGA GATTTTTGCT CACAACCAGC AGCTGAAAGA GCAAAGTCGA 
CAGTTAGAGG AAGAGAATGG AGCATTAAGA AACCACAGCT TGCAGCTGCT GAAGGCACGT 
TGTGAAGAAT TAAAGCTGGA CTGGAATACA CTGTCTTTGG AAAATCTCAT GAAGGAGAAG 
TTGGCCCTAA AAAATCAGAT ATCCGAAAAG CAGAAGCACT GTTTGGAGCT ACAAATCAGC 
ATTGTGGAAC TAGAAAAGTT TCAGAGACAA CAAGAGCTGC TCCAGCTCAA GTCTTCCACC 
TTGCCAGAGG AACCACCACC CAGCCATAAT CGAACCAAAG GTAGCCTTGG CAACCACTTC 
CACGCTGAAC AGTCTTCAGT GCGCATGATG CCTCCTCCAT CTTCTCACAC AAATGGTCTA 
AGCCCTGAGC GGTCCTTGAA TGGTCATGCT TCTCATTATG AGCTGTACCA TTCCTGTCGA 
GGAGGAAGCA GGCCCTGCTC CAAGGAAAGT ACACCATCTC ACTCAGAGCA TGCCGACATT 
GTGCCCAGTA CCCAAACCCA TGCAGCCAAG CACAAAGGAG AGAAAATATC TGGCCCAACC 
CAACCAGACT ACACGCGCTA CTCCCCTGCC AAAATTGCAA TGAGACGGCA CCTGAACCAG 
GAACCCTTAC CCAATGGTAG AAGCACTGAA ATAAATCATA GGTGTAATGT TACAAAAGAC 
CACAGCTTCC CATCCATAAG CCCTGGAATT CCAAATGGTG TCCAATTTAG CCCTCAGGAA 
TCAAATCCTT CTTCCCCTGA GTTAACTCAT ACACCAGGGG AGAGAGGACC TGAAAAGATG 
TTTCGAGAGC GAAATTCCCC TTCTAATCCA GAAACCATCA CCAGTCTGCC TATTAGTATT 
CCCCTGAGCA CAGTCCAGCC TAACAAGCTG CCAGTCAGTA TTCCACTGGC CAGTGTAGTC 
CTGCCAAGTC GTGTGGAAAA GAGAAACAGC CCCAGTCCCG GATGTCACAG CAGAGATGGC 
TCACTTTCAG AGAATCAATA TGGTGCTACT TCTAATAGCC TCAGTTATGC TGCTGTCTCA 
AAACCATTGG CTACCTCAGG TTTCTTCTAT CCAACCTGGT CTTCATCTCT AAATGGACCA 
GTTTTGAATA GCCATGGGTC AGAGACGGAT GGAATGGATG ACTCATCAAA TTCTGGCACA 
TTGTCTAATT CAGGAGGATC ACGGAGCTCC ACCCCTTCCC ACAGTCACCT GATCTCTCAG 
CGCTCCCCTG CCCAACATTC CACATCTGCT AGTCCTTGCA CATTTCCAGA TCCAAGCAAA 
GGTTCTGGTG AAGGGGATAT TGACACAAGT GATGCTGAAA TGGAAGCAAA GCGTCGGATT 
ATCTTCACCA TTACCAGTGG AAGCTCCAAC GGCAGGTCAC CATCAAACAA ACACAGCCCT 
TTGACTGCCA CAATCCGCAT GGAGTGTGGC ACAGGGCAGG ATGCCAAAAG GAGAAGTGGT 
AGACGAAAAA GATCCTCTGC TGGCAGTCAC AATTCAGCTG TATCACCCAA ACGCAGATCT 
CTTCCCACAT CTGTTGGTCT AAACAGTTAT ACTTCAGGAT CTCCACTGAA TATCAGCTCA 
ATGGTGAGTA ACATTAACCA GCCCTTGGAG ATTACTGCAA TCTCTTCCCC TGAAGCCTCT 
ATGAAGAGTT CCCCTGCTTC CTATCAGGAT CATGAGTTGC CCCCAGTTTT AAAAAAGGAA 
AAACCCCTTT CTTTATCTAA TGGCGTTCAT TATTCACCCT TAACAACAGA GGATGAGGCT 
GAGTCCGAGG AAGACACCCA GAGTACAAGA ACGGAGCGAA AGAGTTCCAG CCTGTGCTTT 
GAAAGCAAAG ACTCCCCCCA GAGAAGTGAA GGGGAAAGAG GTCTCCCATA CTCTAGCAAG 
AAGTCTTCAT GTGAGAGTGT AAAATGGAAA GCAACATTCT CTCCAATTTC TGATGCAACT 
CCGGGTCGTG CCTCTGACAG CCCTTCCTCA CCTCACCGTC AGAACACACA TTATTCATCT 
GGAGAGGGAG GCCGCAGAGG ATCAGGGGGA TTGAGTCCTG GCTCTGCAAA TGGCCACAGT 
GACCATCCTA GGGTAGAAGG GGGCAAAGGA GAGTGCAACC TTTTCTTAAG TAAAAAGATG 
TCTTCTGAAG GTAGCAAAAG AGAAGACAGG GGTCCTACAG CAAAACTGGA TCGTTCCAGC 
AGTAAAGTCC CTCGAAGCCG TGAAAGGGAT GGGAACACTG TGTTCATGAC ACAAGGAACT 
GGTGCTCTCA TGAATGGCAA GGGACCTGGC TCAAATCCTT TACACCCACA CCATCATTTT 
TTGAACAGCT TGGCAGCTGG CTCTCAGCTT GCCTTGCAAG GTGGGCTTAA CTCTGTTGCT 
AGCAATTCTG TTCTGCAGTC CATCTTCAGC TCCATCCCTG TACATGTGAC TGGGCCAGCT 
GGACGATTGT CTAACTCTCA CAGCATGTCT GGTTTCACAG CTCCTGGAGG TACTGTAGGA 
GGTATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dot1l ( XM_018096998.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952487.1 Xenopus tropicalis DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X4, mRNA.

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CloneID	OXa42029
Clone ID Related Accession (Same CDS sequence)	XM_018096998.1
Accession Version	XM_018096998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAAG GTGGCAGAAT CGTATCCTCC AAACCATTTG CACCTCTGAA TTTCAGAATA 
AATAGCCGAA ACTTGAGTGA TATTGGCACT ATTATGAGAG TTGTGGAGTT ATCACCTCTT 
AAAGGATCTG TATCTTGGAC TGGGAAGCCT GTTTCTTATT ACCTGCACAC TATAGACCGG 
ACCTTACTTG AAAATTATTT TTCTAGTTTG AAAAATCCAA AACTCAGGGA GGAACAGGAA 
GCAGCAAGAC GCAGACAACA GAGAGAAGGG AAGAGCAATA CCACAACTCC TACAAAAGTT 
CATGAGACAA AGGACTCTGC TGCAGAGGAA GAAAAGTCTG CCCCAGTGAA AAAGGCTTCT 
CCAGCCAAAG CCCGAAGAAA GCTCAGCAAA AAGGGCAGAA AAATGGCAGG TCGCAAGCGG 
GGACGTCCAA AAAAGTTGCT TACCACAACA GCTGACCGCA AGTCCTCTAA ATCACAGACT 
GCACTGGAAA CCCTCCATCC ACAAAATGTC CTTGTTCCTC AGTCTCCAAC AAAATCACCC 
CCTCAGGATT CATACAAAAC ACCTCATAGT CCCTATTATC AGCTACCTCC AAAAGTGCAA 
CGTCAGCCTT CCAATCAGCT TCTGTCATCT CCTACCCCTC CAGCCCTACA GAAATTGCTC 
GATTCATTTA GGATCCAGTA TTTGCAACTT ATGTCTTACA TGAAAACACC CCATTACAAG 
GCTGGTCTGC AGCAACTGCT GGAACAGGAA AAGGAAAAGA ATTCCCACCT TCTTGGTGCT 
GCTCAGCAGC TGCTCACCCA CTGTCAGGCT CAGAAAGAAG AAATAAAGAA ACTTTTTCAG 
CAGAAACTGG ATGAGCTGGG TGTTAAGGCC CTCACTTACA GTGACCTCAT TCAAGCGCAG 
AAAGAGATTT TTGCTCACAA CCAGCAGCTG AAAGAGCAAA GTCGACAGTT AGAGGAAGAG 
AATGGAGCAT TAAGAAACCA CAGCTTGCAG CTGCTGAAGG CACGTTGTGA AGAATTAAAG 
CTGGACTGGA ATACACTGTC TTTGGAAAAT CTCATGAAGG AGAAGTTGGC CCTAAAAAAT 
CAGATATCCG AAAAGCAGAA GCACTGTTTG GAGCTACAAA TCAGCATTGT GGAACTAGAA 
AAGTTTCAGA GACAACAAGA GCTGCTCCAG CTCAAGTCTT CCACCTTGCC AGAGGAACCA 
CCACCCAGCC ATAATCGAAC CAAAGGTAGC CTTGGCAACC ACTTCCACGC TGAACAGTCT 
TCAGTGCGCA TGATGCCTCC TCCATCTTCT CACACAAATG GTCTAAGCCC TGAGCGGTCC 
TTGAATGGTC ATGCTTCTCA TTATGAGCTG TACCATTCCT GTCGAGGAGG AAGCAGGCCC 
TGCTCCAAGG AAAGTACACC ATCTCACTCA GAGCATGCCG ACATTGTGCC CAGTACCCAA 
ACCCATGCAG CCAAGCACAA AGGAGAGAAA ATATCTGGCC CAACCCAACC AGACTACACG 
CGCTACTCCC CTGCCAAAAT TGCAATGAGA CGGCACCTGA ACCAGGAACC CTTACCCAAT 
GGTAGAAGCA CTGAAATAAA TCATAGGTGT AATGTTACAA AAGACCACAG CTTCCCATCC 
ATAAGCCCTG GAATTCCAAA TGGTGTCCAA TTTAGCCCTC AGGAATCAAA TCCTTCTTCC 
CCTGAGTTAA CTCATACACC AGGGGAGAGA GGACCTGAAA AGATGTTTCG AGAGCGAAAT 
TCCCCTTCTA ATCCAGAAAC CATCACCAGT CTGCCTATTA GTATTCCCCT GAGCACAGTC 
CAGCCTAACA AGCTGCCAGT CAGTATTCCA CTGGCCAGTG TAGTCCTGCC AAGTCGTGTG 
GAAAAGAGAA ACAGCCCCAG TCCCGGATGT CACAGCAGAG ATGGCTCACT TTCAGAGAAT 
CAATATGGTG CTACTTCTAA TAGCCTCAGT TATGCTGCTG TCTCAAAACC ATTGGCTACC 
TCAGGTTTCT TCTATCCAAC CTGGTCTTCA TCTCTAAATG GACCAGTTTT GAATAGCCAT 
GGGTCAGAGA CGGATGGAAT GGATGACTCA TCAAATTCTG GCACATTGTC TAATTCAGGA 
GGATCACGGA GCTCCACCCC TTCCCACAGT CACCTGATCT CTCAGCGCTC CCCTGCCCAA 
CATTCCACAT CTGCTAGTCC TTGCACATTT CCAGATCCAA GCAAAGGTTC TGGTGAAGGG 
GATATTGACA CAAGTGATGC TGAAATGGAA GCAAAGCGTC GGATTATCTT CACCATTACC 
AGTGGAAGCT CCAACGGCAG GTCACCATCA AACAAACACA GCCCTTTGAC TGCCACAATC 
CGCATGGAGT GTGGCACAGG GCAGGATGCC AAAAGGAGAA GTGGTAGACG AAAAAGATCC 
TCTGCTGGCA GTCACAATTC AGCTGTATCA CCCAAACGCA GATCTCTTCC CACATCTGTT 
GGTCTAAACA GTTATACTTC AGGATCTCCA CTGAATATCA GCTCAATGGT GAGTAACATT 
AACCAGCCCT TGGAGATTAC TGCAATCTCT TCCCCTGAAG CCTCTATGAA GAGTTCCCCT 
GCTTCCTATC AGGATCATGA GTTGCCCCCA GTTTTAAAAA AGGAAAAACC CCTTTCTTTA 
TCTAATGGCG TTCATTATTC ACCCTTAACA ACAGAGGATG AGGCTGAGTC CGAGGAAGAC 
ACCCAGAGTA CAAGAACGGA GCGAAAGAGT TCCAGCCTGT GCTTTGAAAG CAAAGACTCC 
CCCCAGAGAA GTGAAGGGGA AAGAGGTCTC CCATACTCTA GCAAGAAGTC TTCATGTGAG 
AGTGTAAAAT GGAAAGCAAC ATTCTCTCCA ATTTCTGATG CAACTCCGGG TCGTGCCTCT 
GACAGCCCTT CCTCACCTCA CCGTCAGAAC ACACATTATT CATCTGGAGA GGGAGGCCGC 
AGAGGATCAG GGGGATTGAG TCCTGGCTCT GCAAATGGCC ACAGTGACCA TCCTAGGGTA 
GAAGGGGGCA AAGGAGAGTG CAACCTTTTC TTAAGTAAAA AGATGTCTTC TGAAGGTAGC 
AAAAGAGAAG ACAGGGGTCC TACAGCAAAA CTGGATCGTT CCAGCAGTAA AGTCCCTCGA 
AGCCGTGAAA GGGATGGGAA CACTGTGTTC ATGACACAAG GAACTGGTGC TCTCATGAAT 
GGCAAGGGAC CTGGCTCAAA TCCTTTACAC CCACACCATC ATTTTTTGAA CAGCTTGGCA 
GCTGGCTCTC AGCTTGCCTT GCAAGGTGGG CTTAACTCTG TTGCTAGCAA TTCTGTTCTG 
CAGTCCATCT TCAGCTCCAT CCCTGTACAT GTGACTGGGC CAGCTGGACG ATTGTCTAAC 
TCTCACAGCA TGTCTGGTTT CACAGCTCCT GGAGGTACTG TAGGAGGTAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952487.1
CDS	90..3503
Translation	MKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTLLENYFSSLKNPKLREEQEAARRRQQREGKSNTTTPTKVHETKDSAAEEEKSAPVKKASPAKARRKLSKKGRKMAGRKRGRPKKLLTTTADRKSSKSQTALETLHPQNVLVPQSPTKSPPQDSYKTPHSPYYQLPPKVQRQPSNQLLSSPTPPALQKLLDSFRIQYLQLMSYMKTPHYKAGLQQLLEQEKEKNSHLLGAAQQLLTHCQAQKEEIKKLFQQKLDELGVKALTYSDLIQAQKEIFAHNQQLKEQSRQLEEENGALRNHSLQLLKARCEELKLDWNTLSLENLMKEKLALKNQISEKQKHCLELQISIVELEKFQRQQELLQLKSSTLPEEPPPSHNRTKGSLGNHFHAEQSSVRMMPPPSSHTNGLSPERSLNGHASHYELYHSCRGGSRPCSKESTPSHSEHADIVPSTQTHAAKHKGEKISGPTQPDYTRYSPAKIAMRRHLNQEPLPNGRSTEINHRCNVTKDHSFPSISPGIPNGVQFSPQESNPSSPELTHTPGERGPEKMFRERNSPSNPETITSLPISIPLSTVQPNKLPVSIPLASVVLPSRVEKRNSPSPGCHSRDGSLSENQYGATSNSLSYAAVSKPLATSGFFYPTWSSSLNGPVLNSHGSETDGMDDSSNSGTLSNSGGSRSSTPSHSHLISQRSPAQHSTSASPCTFPDPSKGSGEGDIDTSDAEMEAKRRIIFTITSGSSNGRSPSNKHSPLTATIRMECGTGQDAKRRSGRRKRSSAGSHNSAVSPKRRSLPTSVGLNSYTSGSPLNISSMVSNINQPLEITAISSPEASMKSSPASYQDHELPPVLKKEKPLSLSNGVHYSPLTTEDEAESEEDTQSTRTERKSSSLCFESKDSPQRSEGERGLPYSSKKSSCESVKWKATFSPISDATPGRASDSPSSPHRQNTHYSSGEGGRRGSGGLSPGSANGHSDHPRVEGGKGECNLFLSKKMSSEGSKREDRGPTAKLDRSSSKVPRSRERDGNTVFMTQGTGALMNGKGPGSNPLHPHHHFLNSLAAGSQLALQGGLNSVASNSVLQSIFSSIPVHVTGPAGRLSNSHSMSGFTAPGGTVGGI

Target ORF information:

RefSeq Version	XM_018096998.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096998.1

ORF Insert Sequence:

ATGAAGGAAG GTGGCAGAAT CGTATCCTCC AAACCATTTG CACCTCTGAA TTTCAGAATA 
AATAGCCGAA ACTTGAGTGA TATTGGCACT ATTATGAGAG TTGTGGAGTT ATCACCTCTT 
AAAGGATCTG TATCTTGGAC TGGGAAGCCT GTTTCTTATT ACCTGCACAC TATAGACCGG 
ACCTTACTTG AAAATTATTT TTCTAGTTTG AAAAATCCAA AACTCAGGGA GGAACAGGAA 
GCAGCAAGAC GCAGACAACA GAGAGAAGGG AAGAGCAATA CCACAACTCC TACAAAAGTT 
CATGAGACAA AGGACTCTGC TGCAGAGGAA GAAAAGTCTG CCCCAGTGAA AAAGGCTTCT 
CCAGCCAAAG CCCGAAGAAA GCTCAGCAAA AAGGGCAGAA AAATGGCAGG TCGCAAGCGG 
GGACGTCCAA AAAAGTTGCT TACCACAACA GCTGACCGCA AGTCCTCTAA ATCACAGACT 
GCACTGGAAA CCCTCCATCC ACAAAATGTC CTTGTTCCTC AGTCTCCAAC AAAATCACCC 
CCTCAGGATT CATACAAAAC ACCTCATAGT CCCTATTATC AGCTACCTCC AAAAGTGCAA 
CGTCAGCCTT CCAATCAGCT TCTGTCATCT CCTACCCCTC CAGCCCTACA GAAATTGCTC 
GATTCATTTA GGATCCAGTA TTTGCAACTT ATGTCTTACA TGAAAACACC CCATTACAAG 
GCTGGTCTGC AGCAACTGCT GGAACAGGAA AAGGAAAAGA ATTCCCACCT TCTTGGTGCT 
GCTCAGCAGC TGCTCACCCA CTGTCAGGCT CAGAAAGAAG AAATAAAGAA ACTTTTTCAG 
CAGAAACTGG ATGAGCTGGG TGTTAAGGCC CTCACTTACA GTGACCTCAT TCAAGCGCAG 
AAAGAGATTT TTGCTCACAA CCAGCAGCTG AAAGAGCAAA GTCGACAGTT AGAGGAAGAG 
AATGGAGCAT TAAGAAACCA CAGCTTGCAG CTGCTGAAGG CACGTTGTGA AGAATTAAAG 
CTGGACTGGA ATACACTGTC TTTGGAAAAT CTCATGAAGG AGAAGTTGGC CCTAAAAAAT 
CAGATATCCG AAAAGCAGAA GCACTGTTTG GAGCTACAAA TCAGCATTGT GGAACTAGAA 
AAGTTTCAGA GACAACAAGA GCTGCTCCAG CTCAAGTCTT CCACCTTGCC AGAGGAACCA 
CCACCCAGCC ATAATCGAAC CAAAGGTAGC CTTGGCAACC ACTTCCACGC TGAACAGTCT 
TCAGTGCGCA TGATGCCTCC TCCATCTTCT CACACAAATG GTCTAAGCCC TGAGCGGTCC 
TTGAATGGTC ATGCTTCTCA TTATGAGCTG TACCATTCCT GTCGAGGAGG AAGCAGGCCC 
TGCTCCAAGG AAAGTACACC ATCTCACTCA GAGCATGCCG ACATTGTGCC CAGTACCCAA 
ACCCATGCAG CCAAGCACAA AGGAGAGAAA ATATCTGGCC CAACCCAACC AGACTACACG 
CGCTACTCCC CTGCCAAAAT TGCAATGAGA CGGCACCTGA ACCAGGAACC CTTACCCAAT 
GGTAGAAGCA CTGAAATAAA TCATAGGTGT AATGTTACAA AAGACCACAG CTTCCCATCC 
ATAAGCCCTG GAATTCCAAA TGGTGTCCAA TTTAGCCCTC AGGAATCAAA TCCTTCTTCC 
CCTGAGTTAA CTCATACACC AGGGGAGAGA GGACCTGAAA AGATGTTTCG AGAGCGAAAT 
TCCCCTTCTA ATCCAGAAAC CATCACCAGT CTGCCTATTA GTATTCCCCT GAGCACAGTC 
CAGCCTAACA AGCTGCCAGT CAGTATTCCA CTGGCCAGTG TAGTCCTGCC AAGTCGTGTG 
GAAAAGAGAA ACAGCCCCAG TCCCGGATGT CACAGCAGAG ATGGCTCACT TTCAGAGAAT 
CAATATGGTG CTACTTCTAA TAGCCTCAGT TATGCTGCTG TCTCAAAACC ATTGGCTACC 
TCAGGTTTCT TCTATCCAAC CTGGTCTTCA TCTCTAAATG GACCAGTTTT GAATAGCCAT 
GGGTCAGAGA CGGATGGAAT GGATGACTCA TCAAATTCTG GCACATTGTC TAATTCAGGA 
GGATCACGGA GCTCCACCCC TTCCCACAGT CACCTGATCT CTCAGCGCTC CCCTGCCCAA 
CATTCCACAT CTGCTAGTCC TTGCACATTT CCAGATCCAA GCAAAGGTTC TGGTGAAGGG 
GATATTGACA CAAGTGATGC TGAAATGGAA GCAAAGCGTC GGATTATCTT CACCATTACC 
AGTGGAAGCT CCAACGGCAG GTCACCATCA AACAAACACA GCCCTTTGAC TGCCACAATC 
CGCATGGAGT GTGGCACAGG GCAGGATGCC AAAAGGAGAA GTGGTAGACG AAAAAGATCC 
TCTGCTGGCA GTCACAATTC AGCTGTATCA CCCAAACGCA GATCTCTTCC CACATCTGTT 
GGTCTAAACA GTTATACTTC AGGATCTCCA CTGAATATCA GCTCAATGGT GAGTAACATT 
AACCAGCCCT TGGAGATTAC TGCAATCTCT TCCCCTGAAG CCTCTATGAA GAGTTCCCCT 
GCTTCCTATC AGGATCATGA GTTGCCCCCA GTTTTAAAAA AGGAAAAACC CCTTTCTTTA 
TCTAATGGCG TTCATTATTC ACCCTTAACA ACAGAGGATG AGGCTGAGTC CGAGGAAGAC 
ACCCAGAGTA CAAGAACGGA GCGAAAGAGT TCCAGCCTGT GCTTTGAAAG CAAAGACTCC 
CCCCAGAGAA GTGAAGGGGA AAGAGGTCTC CCATACTCTA GCAAGAAGTC TTCATGTGAG 
AGTGTAAAAT GGAAAGCAAC ATTCTCTCCA ATTTCTGATG CAACTCCGGG TCGTGCCTCT 
GACAGCCCTT CCTCACCTCA CCGTCAGAAC ACACATTATT CATCTGGAGA GGGAGGCCGC 
AGAGGATCAG GGGGATTGAG TCCTGGCTCT GCAAATGGCC ACAGTGACCA TCCTAGGGTA 
GAAGGGGGCA AAGGAGAGTG CAACCTTTTC TTAAGTAAAA AGATGTCTTC TGAAGGTAGC 
AAAAGAGAAG ACAGGGGTCC TACAGCAAAA CTGGATCGTT CCAGCAGTAA AGTCCCTCGA 
AGCCGTGAAA GGGATGGGAA CACTGTGTTC ATGACACAAG GAACTGGTGC TCTCATGAAT 
GGCAAGGGAC CTGGCTCAAA TCCTTTACAC CCACACCATC ATTTTTTGAA CAGCTTGGCA 
GCTGGCTCTC AGCTTGCCTT GCAAGGTGGG CTTAACTCTG TTGCTAGCAA TTCTGTTCTG 
CAGTCCATCT TCAGCTCCAT CCCTGTACAT GTGACTGGGC CAGCTGGACG ATTGTCTAAC 
TCTCACAGCA TGTCTGGTTT CACAGCTCCT GGAGGTACTG TAGGAGGTAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dot1l ( XM_012954295.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809749.1 Xenopus tropicalis DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X1, mRNA.

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CloneID	OXa48512
Clone ID Related Accession (Same CDS sequence)	XM_012954295.3
Accession Version	XM_012954295.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012954295.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGAGA AGCTGGAACT AAGGCTGAAG TCCCCAGTGG GAGCCGAGCC TGCCGTCTAC 
CCTTGGCCGC TGCCTGTTTA CGATAAACAC CATGATGCTG CACATGAAAT TATTGAAACC 
ATCAGATGGG TCTGTGAAGA AATTCCAGAT CTCAAGCTTG CAATGGAAAA CTATGTTCTG 
ATAGACTATG ACACAAAGTG CTTTGAGAGC ATGCAGCGGC TGTGTGAGAA GTATAACAGA 
GCCATTGACA GCATCCATCA GCTGTGGAAA GGCACCACTC ATCCTATGAA GTTGAAAACT 
CGCCCTTCTA CTGGTCTCCT TCGCCACATT TTGCAGCAGG TCTACAACCA CTCTGTAACA 
GACCCTGAAA AACTCAACAA TTATGAGCCG TTTTCTCCTG AGGTCTATGG AGAAACCTCC 
TTTGATTTGG TTGCCCAGAT GATTGATGAA ATTAAGATGA CTGAGGATGA TCTCTTTGTG 
GACCTGGGAA GTGGTGTCGG GCAGGTTGTT CTGCAGGTTG CTGCAGCAAC AGGTTGCAAG 
CATCATTATG GTGTAGAGAA AGCGGATATC CCAGCCAAGT ATGCAGAGAC AATGGACACT 
GAGTTCAGGA AATGGATGAA GTGGTATGGA AAGAAGCATT CAGAGTACAC ACTGGAGCGT 
GGAGACTTCC TCTCAGAGGA GTGGAGGGAA AGAATAGCTA ACACAAGTGT TATTTTTGTG 
AATAATTTTG CCTTTGGTCC TGAGGTTGAC CACCAGCTAA AGGAGAGATT CGCCAACATG 
AAGGAAGGTG GCAGAATCGT ATCCTCCAAA CCATTTGCAC CTCTGAATTT CAGAATAAAT 
AGCCGAAACT TGAGTGATAT TGGCACTATT ATGAGAGTTG TGGAGTTATC ACCTCTTAAA 
GGATCTGTAT CTTGGACTGG GAAGCCTGTT TCTTATTACC TGCACACTAT AGACCGGACC 
TTACTTGAAA ATTATTTTTC TAGTTTGAAA AATCCAAAAC TCAGGGAGGA ACAGGAAGCA 
GCAAGACGCA GACAACAGAG AGAAGGGAAG AGCAATACCA CAACTCCTAC AAAAGTTCAT 
GAGACAAAGG ACTCTGCTGC AGAGGAAGAA AAGTCTGCCC CAGTGAAAAA GGCTTCTCCA 
GCCAAAGCCC GAAGAAAGCT CAGCAAAAAG GGCAGAAAAA TGGCAGGTCG CAAGCGGGGA 
CGTCCAAAAA AGTTGCTTAC CACAACAGCT GACCGCAAGT CCTCTAAATC ACAGACTGCA 
CTGGAAACCC TCCATCCACA AAATGTCCTT GTTCCTCAGT CTCCAACAAA ATCACCCCCT 
CAGGATTCAT ACAAAACACC TCATAGTCCC TATTATCAGC TACCTCCAAA AGTGCAACGT 
CAGCCTTCCA ATCAGCTTCT GTCATCTCCT ACCCCTCCAG CCCTACAGAA ATTGCTCGAT 
TCATTTAGGA TCCAGTATTT GCAACTTATG TCTTACATGA AAACACCCCA TTACAAGGCT 
GGTCTGCAGC AACTGCTGGA ACAGGAAAAG GAAAAGAATT CCCACCTTCT TGGTGCTGCT 
CAGCAGCTGC TCACCCACTG TCAGGCTCAG AAAGAAGAAA TAAAGAAACT TTTTCAGCAG 
AAACTGGATG AGCTGGGTGT TAAGGCCCTC ACTTACAGTG ACCTCATTCA AGCGCAGAAA 
GAGATTTTTG CTCACAACCA GCAGCTGAAA GAGCAAAGTC GACAGTTAGA GGAAGAGAAT 
GGAGCATTAA GAAACCACAG CTTGCAGCTG CTGAAGGCAC GTTGTGAAGA ATTAAAGCTG 
GACTGGAATA CACTGTCTTT GGAAAATCTC ATGAAGGAGA AGTTGGCCCT AAAAAATCAG 
ATATCCGAAA AGCAGAAGCA CTGTTTGGAG CTACAAATCA GCATTGTGGA ACTAGAAAAG 
TTTCAGAGAC AACAAGAGCT GCTCCAGCTC AAGTCTTCCA CCTTGCCAGA GGAACCACCA 
CCCAGCCATA ATCGAACCAA AGGTAGCCTT GGCAACCACT TCCACGCTGA ACAGTCTTCA 
GTGCGCATGA TGCCTCCTCC ATCTTCTCAC ACAAATGGTC TAAGCCCTGA GCGGTCCTTG 
AATGGTCATG CTTCTCATTA TGAGCTGTAC CATTCCTGTC GAGGAGGAAG CAGGCCCTGC 
TCCAAGGAAA GTACACCATC TCACTCAGAG CATGCCGACA TTGTGCCCAG TACCCAAACC 
CATGCAGCCA AGCACAAAGG AGAGAAAATA TCTGGCCCAA CCCAACCAGA CTACACGCGC 
TACTCCCCTG CCAAAATTGC AATGAGACGG CACCTGAACC AGGAACCCTT ACCCAATGGT 
AGAAGCACTG AAATAAATCA TAGGTGTAAT GTTACAAAAG ACCACAGCTT CCCATCCATA 
AGCCCTGGAA TTCCAAATGG TGTCCAATTT AGCCCTCAGG AATCAAATCC TTCTTCCCCT 
GAGTTAACTC ATACACCAGG GGAGAGAGGA CCTGAAAAGA TGTTTCGAGA GCGAAATTCC 
CCTTCTAATC CAGAAACCAT CACCAGTCTG CCTATTAGTA TTCCCCTGAG CACAGTCCAG 
CCTAACAAGC TGCCAGTCAG TATTCCACTG GCCAGTGTAG TCCTGCCAAG TCGTGTGGAA 
AAGAGAAACA GCCCCAGTCC CGGATGTCAC AGCAGAGATG GCTCACTTTC AGAGAATCAA 
TATGGTGCTA CTTCTAATAG CCTCAGTTAT GCTGCTGTCT CAAAACCATT GGCTACCTCA 
GGTTTCTTCT ATCCAACCTG GTCTTCATCT CTAAATGGAC CAGTTTTGAA TAGCCATGGG 
TCAGAGACGG ATGGAATGGA TGACTCATCA AATTCTGGCA CATTGTCTAA TTCAGGAGGA 
TCACGGAGCT CCACCCCTTC CCACAGTCAC CTGATCTCTC AGCGCTCCCC TGCCCAACAT 
TCCACATCTG CTAGTCCTTG CACATTTCCA GATCCAAGCA AAGGTTCTGG TGAAGGGGAT 
ATTGACACAA GTGATGCTGA AATGGAAGCA AAGCGTCGGA TTATCTTCAC CATTACCAGT 
GGAAGCTCCA ACGGCAGGTC ACCATCAAAC AAACACAGCC CTTTGACTGC CACAATCCGC 
ATGGAGTGTG GCACAGGGCA GGATGCCAAA AGGAGAAGTG GTAGACGAAA AAGATCCTCT 
GCTGGCAGTC ACAATTCAGC TGTATCACCC AAACGCAGAT CTCTTCCCAC ATCTGTTGGT 
CTAAACAGTT ATACTTCAGG ATCTCCACTG AATATCAGCT CAATGGTGAG TAACATTAAC 
CAGCCCTTGG AGATTACTGC AATCTCTTCC CCTGAAGCCT CTATGAAGAG TTCCCCTGCT 
TCCTATCAGG ATCATGAGTT GCCCCCAGTT TTAAAAAAGG AAAAACCCCT TTCTTTATCT 
AATGGCGTTC ATTATTCACC CTTAACAACA GAGGATGAGG CTGAGTCTGA GGAAGACACC 
CAGAGTACAA GAACGGAGCG AAAGAGTTCC AGCCTGTGCT TTGAAAGCAA AGACTCCCCC 
CAGAGAAGTG AAGGGGAAAG AGGTCTCCCA TACTCTAGCA AGAAGTCTTC ATGTGAGAGT 
GTAAAATGGA AAGCAACATT CTCTCCAATT TCTGATGCAA CTCCGGGTCG TGCCTCTGAC 
AGCCCTTCCT CACCTCACCG TCAGAACACA CATTATTCAT CTGGAGAGGG AGGCCGCAGA 
GGATCAGGGG GATTGAGTCC TGGCTCTGCA AATGGCCACA GTGACCATCC TAGGGTAGAA 
GGGGGCAAAG GAGAGTGCAA CCTTTTCTTA AGTAAAAAGA TGTCTTCTGA AGGTAGCAAA 
AGAGAAGACA GGGGTCCTAC AGCAAAACTG GATCGTTCCA GCAGTAAAGT CCCTCGAAGC 
CGTGAAAGGG ATGGGAACAC TGTGTTCATG ACACAAGGAA CTGGTGCTCT CATGAATGGC 
AAGGGACCTG GCTCAAATCC TTTACACCCA CACCATCATT TTTTGAACAG CTTGGCAGCT 
GGCTCTCAGC TTGCCTTGCA AGGTGGGCTT AACTCTGTTG CTAGCAATTC TGTTCTGCAG 
TCCATCTTCA GCTCCATCCC TGTACATGTG ACTGGGCCAG CTGGACGATT GTCTAACTCT 
CACAGCATGT CTGGTTTCAC AGCTCCTGGA GGTACTGTAG GAGGTATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809749.1
CDS	538..4728
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKCFESMQRLCEKYNRAIDSIHQLWKGTTHPMKLKTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATGCKHHYGVEKADIPAKYAETMDTEFRKWMKWYGKKHSEYTLERGDFLSEEWRERIANTSVIFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTLLENYFSSLKNPKLREEQEAARRRQQREGKSNTTTPTKVHETKDSAAEEEKSAPVKKASPAKARRKLSKKGRKMAGRKRGRPKKLLTTTADRKSSKSQTALETLHPQNVLVPQSPTKSPPQDSYKTPHSPYYQLPPKVQRQPSNQLLSSPTPPALQKLLDSFRIQYLQLMSYMKTPHYKAGLQQLLEQEKEKNSHLLGAAQQLLTHCQAQKEEIKKLFQQKLDELGVKALTYSDLIQAQKEIFAHNQQLKEQSRQLEEENGALRNHSLQLLKARCEELKLDWNTLSLENLMKEKLALKNQISEKQKHCLELQISIVELEKFQRQQELLQLKSSTLPEEPPPSHNRTKGSLGNHFHAEQSSVRMMPPPSSHTNGLSPERSLNGHASHYELYHSCRGGSRPCSKESTPSHSEHADIVPSTQTHAAKHKGEKISGPTQPDYTRYSPAKIAMRRHLNQEPLPNGRSTEINHRCNVTKDHSFPSISPGIPNGVQFSPQESNPSSPELTHTPGERGPEKMFRERNSPSNPETITSLPISIPLSTVQPNKLPVSIPLASVVLPSRVEKRNSPSPGCHSRDGSLSENQYGATSNSLSYAAVSKPLATSGFFYPTWSSSLNGPVLNSHGSETDGMDDSSNSGTLSNSGGSRSSTPSHSHLISQRSPAQHSTSASPCTFPDPSKGSGEGDIDTSDAEMEAKRRIIFTITSGSSNGRSPSNKHSPLTATIRMECGTGQDAKRRSGRRKRSSAGSHNSAVSPKRRSLPTSVGLNSYTSGSPLNISSMVSNINQPLEITAISSPEASMKSSPASYQDHELPPVLKKEKPLSLSNGVHYSPLTTEDEAESEEDTQSTRTERKSSSLCFESKDSPQRSEGERGLPYSSKKSSCESVKWKATFSPISDATPGRASDSPSSPHRQNTHYSSGEGGRRGSGGLSPGSANGHSDHPRVEGGKGECNLFLSKKMSSEGSKREDRGPTAKLDRSSSKVPRSRERDGNTVFMTQGTGALMNGKGPGSNPLHPHHHFLNSLAAGSQLALQGGLNSVASNSVLQSIFSSIPVHVTGPAGRLSNSHSMSGFTAPGGTVGGI

Target ORF information:

RefSeq Version	XM_012954295.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012954295.3

ORF Insert Sequence:

ATGGGCGAGA AGCTGGAACT AAGGCTGAAG TCCCCAGTGG GAGCCGAGCC TGCCGTCTAC 
CCTTGGCCGC TGCCTGTTTA CGATAAACAC CATGATGCTG CACATGAAAT TATTGAAACC 
ATCAGATGGG TCTGTGAAGA AATTCCAGAT CTCAAGCTTG CAATGGAAAA CTATGTTCTG 
ATAGACTATG ACACAAAGTG CTTTGAGAGC ATGCAGCGGC TGTGTGAGAA GTATAACAGA 
GCCATTGACA GCATCCATCA GCTGTGGAAA GGCACCACTC ATCCTATGAA GTTGAAAACT 
CGCCCTTCTA CTGGTCTCCT TCGCCACATT TTGCAGCAGG TCTACAACCA CTCTGTAACA 
GACCCTGAAA AACTCAACAA TTATGAGCCG TTTTCTCCTG AGGTCTATGG AGAAACCTCC 
TTTGATTTGG TTGCCCAGAT GATTGATGAA ATTAAGATGA CTGAGGATGA TCTCTTTGTG 
GACCTGGGAA GTGGTGTCGG GCAGGTTGTT CTGCAGGTTG CTGCAGCAAC AGGTTGCAAG 
CATCATTATG GTGTAGAGAA AGCGGATATC CCAGCCAAGT ATGCAGAGAC AATGGACACT 
GAGTTCAGGA AATGGATGAA GTGGTATGGA AAGAAGCATT CAGAGTACAC ACTGGAGCGT 
GGAGACTTCC TCTCAGAGGA GTGGAGGGAA AGAATAGCTA ACACAAGTGT TATTTTTGTG 
AATAATTTTG CCTTTGGTCC TGAGGTTGAC CACCAGCTAA AGGAGAGATT CGCCAACATG 
AAGGAAGGTG GCAGAATCGT ATCCTCCAAA CCATTTGCAC CTCTGAATTT CAGAATAAAT 
AGCCGAAACT TGAGTGATAT TGGCACTATT ATGAGAGTTG TGGAGTTATC ACCTCTTAAA 
GGATCTGTAT CTTGGACTGG GAAGCCTGTT TCTTATTACC TGCACACTAT AGACCGGACC 
TTACTTGAAA ATTATTTTTC TAGTTTGAAA AATCCAAAAC TCAGGGAGGA ACAGGAAGCA 
GCAAGACGCA GACAACAGAG AGAAGGGAAG AGCAATACCA CAACTCCTAC AAAAGTTCAT 
GAGACAAAGG ACTCTGCTGC AGAGGAAGAA AAGTCTGCCC CAGTGAAAAA GGCTTCTCCA 
GCCAAAGCCC GAAGAAAGCT CAGCAAAAAG GGCAGAAAAA TGGCAGGTCG CAAGCGGGGA 
CGTCCAAAAA AGTTGCTTAC CACAACAGCT GACCGCAAGT CCTCTAAATC ACAGACTGCA 
CTGGAAACCC TCCATCCACA AAATGTCCTT GTTCCTCAGT CTCCAACAAA ATCACCCCCT 
CAGGATTCAT ACAAAACACC TCATAGTCCC TATTATCAGC TACCTCCAAA AGTGCAACGT 
CAGCCTTCCA ATCAGCTTCT GTCATCTCCT ACCCCTCCAG CCCTACAGAA ATTGCTCGAT 
TCATTTAGGA TCCAGTATTT GCAACTTATG TCTTACATGA AAACACCCCA TTACAAGGCT 
GGTCTGCAGC AACTGCTGGA ACAGGAAAAG GAAAAGAATT CCCACCTTCT TGGTGCTGCT 
CAGCAGCTGC TCACCCACTG TCAGGCTCAG AAAGAAGAAA TAAAGAAACT TTTTCAGCAG 
AAACTGGATG AGCTGGGTGT TAAGGCCCTC ACTTACAGTG ACCTCATTCA AGCGCAGAAA 
GAGATTTTTG CTCACAACCA GCAGCTGAAA GAGCAAAGTC GACAGTTAGA GGAAGAGAAT 
GGAGCATTAA GAAACCACAG CTTGCAGCTG CTGAAGGCAC GTTGTGAAGA ATTAAAGCTG 
GACTGGAATA CACTGTCTTT GGAAAATCTC ATGAAGGAGA AGTTGGCCCT AAAAAATCAG 
ATATCCGAAA AGCAGAAGCA CTGTTTGGAG CTACAAATCA GCATTGTGGA ACTAGAAAAG 
TTTCAGAGAC AACAAGAGCT GCTCCAGCTC AAGTCTTCCA CCTTGCCAGA GGAACCACCA 
CCCAGCCATA ATCGAACCAA AGGTAGCCTT GGCAACCACT TCCACGCTGA ACAGTCTTCA 
GTGCGCATGA TGCCTCCTCC ATCTTCTCAC ACAAATGGTC TAAGCCCTGA GCGGTCCTTG 
AATGGTCATG CTTCTCATTA TGAGCTGTAC CATTCCTGTC GAGGAGGAAG CAGGCCCTGC 
TCCAAGGAAA GTACACCATC TCACTCAGAG CATGCCGACA TTGTGCCCAG TACCCAAACC 
CATGCAGCCA AGCACAAAGG AGAGAAAATA TCTGGCCCAA CCCAACCAGA CTACACGCGC 
TACTCCCCTG CCAAAATTGC AATGAGACGG CACCTGAACC AGGAACCCTT ACCCAATGGT 
AGAAGCACTG AAATAAATCA TAGGTGTAAT GTTACAAAAG ACCACAGCTT CCCATCCATA 
AGCCCTGGAA TTCCAAATGG TGTCCAATTT AGCCCTCAGG AATCAAATCC TTCTTCCCCT 
GAGTTAACTC ATACACCAGG GGAGAGAGGA CCTGAAAAGA TGTTTCGAGA GCGAAATTCC 
CCTTCTAATC CAGAAACCAT CACCAGTCTG CCTATTAGTA TTCCCCTGAG CACAGTCCAG 
CCTAACAAGC TGCCAGTCAG TATTCCACTG GCCAGTGTAG TCCTGCCAAG TCGTGTGGAA 
AAGAGAAACA GCCCCAGTCC CGGATGTCAC AGCAGAGATG GCTCACTTTC AGAGAATCAA 
TATGGTGCTA CTTCTAATAG CCTCAGTTAT GCTGCTGTCT CAAAACCATT GGCTACCTCA 
GGTTTCTTCT ATCCAACCTG GTCTTCATCT CTAAATGGAC CAGTTTTGAA TAGCCATGGG 
TCAGAGACGG ATGGAATGGA TGACTCATCA AATTCTGGCA CATTGTCTAA TTCAGGAGGA 
TCACGGAGCT CCACCCCTTC CCACAGTCAC CTGATCTCTC AGCGCTCCCC TGCCCAACAT 
TCCACATCTG CTAGTCCTTG CACATTTCCA GATCCAAGCA AAGGTTCTGG TGAAGGGGAT 
ATTGACACAA GTGATGCTGA AATGGAAGCA AAGCGTCGGA TTATCTTCAC CATTACCAGT 
GGAAGCTCCA ACGGCAGGTC ACCATCAAAC AAACACAGCC CTTTGACTGC CACAATCCGC 
ATGGAGTGTG GCACAGGGCA GGATGCCAAA AGGAGAAGTG GTAGACGAAA AAGATCCTCT 
GCTGGCAGTC ACAATTCAGC TGTATCACCC AAACGCAGAT CTCTTCCCAC ATCTGTTGGT 
CTAAACAGTT ATACTTCAGG ATCTCCACTG AATATCAGCT CAATGGTGAG TAACATTAAC 
CAGCCCTTGG AGATTACTGC AATCTCTTCC CCTGAAGCCT CTATGAAGAG TTCCCCTGCT 
TCCTATCAGG ATCATGAGTT GCCCCCAGTT TTAAAAAAGG AAAAACCCCT TTCTTTATCT 
AATGGCGTTC ATTATTCACC CTTAACAACA GAGGATGAGG CTGAGTCTGA GGAAGACACC 
CAGAGTACAA GAACGGAGCG AAAGAGTTCC AGCCTGTGCT TTGAAAGCAA AGACTCCCCC 
CAGAGAAGTG AAGGGGAAAG AGGTCTCCCA TACTCTAGCA AGAAGTCTTC ATGTGAGAGT 
GTAAAATGGA AAGCAACATT CTCTCCAATT TCTGATGCAA CTCCGGGTCG TGCCTCTGAC 
AGCCCTTCCT CACCTCACCG TCAGAACACA CATTATTCAT CTGGAGAGGG AGGCCGCAGA 
GGATCAGGGG GATTGAGTCC TGGCTCTGCA AATGGCCACA GTGACCATCC TAGGGTAGAA 
GGGGGCAAAG GAGAGTGCAA CCTTTTCTTA AGTAAAAAGA TGTCTTCTGA AGGTAGCAAA 
AGAGAAGACA GGGGTCCTAC AGCAAAACTG GATCGTTCCA GCAGTAAAGT CCCTCGAAGC 
CGTGAAAGGG ATGGGAACAC TGTGTTCATG ACACAAGGAA CTGGTGCTCT CATGAATGGC 
AAGGGACCTG GCTCAAATCC TTTACACCCA CACCATCATT TTTTGAACAG CTTGGCAGCT 
GGCTCTCAGC TTGCCTTGCA AGGTGGGCTT AACTCTGTTG CTAGCAATTC TGTTCTGCAG 
TCCATCTTCA GCTCCATCCC TGTACATGTG ACTGGGCCAG CTGGACGATT GTCTAACTCT 
CACAGCATGT CTGGTTTCAC AGCTCCTGGA GGTACTGTAG GAGGTATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dot1l ( XM_018096998.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952487.1 Xenopus tropicalis DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa48513
Clone ID Related Accession (Same CDS sequence)	XM_018096998.2
Accession Version	XM_018096998.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096998.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAAG GTGGCAGAAT CGTATCCTCC AAACCATTTG CACCTCTGAA TTTCAGAATA 
AATAGCCGAA ACTTGAGTGA TATTGGCACT ATTATGAGAG TTGTGGAGTT ATCACCTCTT 
AAAGGATCTG TATCTTGGAC TGGGAAGCCT GTTTCTTATT ACCTGCACAC TATAGACCGG 
ACCTTACTTG AAAATTATTT TTCTAGTTTG AAAAATCCAA AACTCAGGGA GGAACAGGAA 
GCAGCAAGAC GCAGACAACA GAGAGAAGGG AAGAGCAATA CCACAACTCC TACAAAAGTT 
CATGAGACAA AGGACTCTGC TGCAGAGGAA GAAAAGTCTG CCCCAGTGAA AAAGGCTTCT 
CCAGCCAAAG CCCGAAGAAA GCTCAGCAAA AAGGGCAGAA AAATGGCAGG TCGCAAGCGG 
GGACGTCCAA AAAAGTTGCT TACCACAACA GCTGACCGCA AGTCCTCTAA ATCACAGACT 
GCACTGGAAA CCCTCCATCC ACAAAATGTC CTTGTTCCTC AGTCTCCAAC AAAATCACCC 
CCTCAGGATT CATACAAAAC ACCTCATAGT CCCTATTATC AGCTACCTCC AAAAGTGCAA 
CGTCAGCCTT CCAATCAGCT TCTGTCATCT CCTACCCCTC CAGCCCTACA GAAATTGCTC 
GATTCATTTA GGATCCAGTA TTTGCAACTT ATGTCTTACA TGAAAACACC CCATTACAAG 
GCTGGTCTGC AGCAACTGCT GGAACAGGAA AAGGAAAAGA ATTCCCACCT TCTTGGTGCT 
GCTCAGCAGC TGCTCACCCA CTGTCAGGCT CAGAAAGAAG AAATAAAGAA ACTTTTTCAG 
CAGAAACTGG ATGAGCTGGG TGTTAAGGCC CTCACTTACA GTGACCTCAT TCAAGCGCAG 
AAAGAGATTT TTGCTCACAA CCAGCAGCTG AAAGAGCAAA GTCGACAGTT AGAGGAAGAG 
AATGGAGCAT TAAGAAACCA CAGCTTGCAG CTGCTGAAGG CACGTTGTGA AGAATTAAAG 
CTGGACTGGA ATACACTGTC TTTGGAAAAT CTCATGAAGG AGAAGTTGGC CCTAAAAAAT 
CAGATATCCG AAAAGCAGAA GCACTGTTTG GAGCTACAAA TCAGCATTGT GGAACTAGAA 
AAGTTTCAGA GACAACAAGA GCTGCTCCAG CTCAAGTCTT CCACCTTGCC AGAGGAACCA 
CCACCCAGCC ATAATCGAAC CAAAGGTAGC CTTGGCAACC ACTTCCACGC TGAACAGTCT 
TCAGTGCGCA TGATGCCTCC TCCATCTTCT CACACAAATG GTCTAAGCCC TGAGCGGTCC 
TTGAATGGTC ATGCTTCTCA TTATGAGCTG TACCATTCCT GTCGAGGAGG AAGCAGGCCC 
TGCTCCAAGG AAAGTACACC ATCTCACTCA GAGCATGCCG ACATTGTGCC CAGTACCCAA 
ACCCATGCAG CCAAGCACAA AGGAGAGAAA ATATCTGGCC CAACCCAACC AGACTACACG 
CGCTACTCCC CTGCCAAAAT TGCAATGAGA CGGCACCTGA ACCAGGAACC CTTACCCAAT 
GGTAGAAGCA CTGAAATAAA TCATAGGTGT AATGTTACAA AAGACCACAG CTTCCCATCC 
ATAAGCCCTG GAATTCCAAA TGGTGTCCAA TTTAGCCCTC AGGAATCAAA TCCTTCTTCC 
CCTGAGTTAA CTCATACACC AGGGGAGAGA GGACCTGAAA AGATGTTTCG AGAGCGAAAT 
TCCCCTTCTA ATCCAGAAAC CATCACCAGT CTGCCTATTA GTATTCCCCT GAGCACAGTC 
CAGCCTAACA AGCTGCCAGT CAGTATTCCA CTGGCCAGTG TAGTCCTGCC AAGTCGTGTG 
GAAAAGAGAA ACAGCCCCAG TCCCGGATGT CACAGCAGAG ATGGCTCACT TTCAGAGAAT 
CAATATGGTG CTACTTCTAA TAGCCTCAGT TATGCTGCTG TCTCAAAACC ATTGGCTACC 
TCAGGTTTCT TCTATCCAAC CTGGTCTTCA TCTCTAAATG GACCAGTTTT GAATAGCCAT 
GGGTCAGAGA CGGATGGAAT GGATGACTCA TCAAATTCTG GCACATTGTC TAATTCAGGA 
GGATCACGGA GCTCCACCCC TTCCCACAGT CACCTGATCT CTCAGCGCTC CCCTGCCCAA 
CATTCCACAT CTGCTAGTCC TTGCACATTT CCAGATCCAA GCAAAGGTTC TGGTGAAGGG 
GATATTGACA CAAGTGATGC TGAAATGGAA GCAAAGCGTC GGATTATCTT CACCATTACC 
AGTGGAAGCT CCAACGGCAG GTCACCATCA AACAAACACA GCCCTTTGAC TGCCACAATC 
CGCATGGAGT GTGGCACAGG GCAGGATGCC AAAAGGAGAA GTGGTAGACG AAAAAGATCC 
TCTGCTGGCA GTCACAATTC AGCTGTATCA CCCAAACGCA GATCTCTTCC CACATCTGTT 
GGTCTAAACA GTTATACTTC AGGATCTCCA CTGAATATCA GCTCAATGGT GAGTAACATT 
AACCAGCCCT TGGAGATTAC TGCAATCTCT TCCCCTGAAG CCTCTATGAA GAGTTCCCCT 
GCTTCCTATC AGGATCATGA GTTGCCCCCA GTTTTAAAAA AGGAAAAACC CCTTTCTTTA 
TCTAATGGCG TTCATTATTC ACCCTTAACA ACAGAGGATG AGGCTGAGTC TGAGGAAGAC 
ACCCAGAGTA CAAGAACGGA GCGAAAGAGT TCCAGCCTGT GCTTTGAAAG CAAAGACTCC 
CCCCAGAGAA GTGAAGGGGA AAGAGGTCTC CCATACTCTA GCAAGAAGTC TTCATGTGAG 
AGTGTAAAAT GGAAAGCAAC ATTCTCTCCA ATTTCTGATG CAACTCCGGG TCGTGCCTCT 
GACAGCCCTT CCTCACCTCA CCGTCAGAAC ACACATTATT CATCTGGAGA GGGAGGCCGC 
AGAGGATCAG GGGGATTGAG TCCTGGCTCT GCAAATGGCC ACAGTGACCA TCCTAGGGTA 
GAAGGGGGCA AAGGAGAGTG CAACCTTTTC TTAAGTAAAA AGATGTCTTC TGAAGGTAGC 
AAAAGAGAAG ACAGGGGTCC TACAGCAAAA CTGGATCGTT CCAGCAGTAA AGTCCCTCGA 
AGCCGTGAAA GGGATGGGAA CACTGTGTTC ATGACACAAG GAACTGGTGC TCTCATGAAT 
GGCAAGGGAC CTGGCTCAAA TCCTTTACAC CCACACCATC ATTTTTTGAA CAGCTTGGCA 
GCTGGCTCTC AGCTTGCCTT GCAAGGTGGG CTTAACTCTG TTGCTAGCAA TTCTGTTCTG 
CAGTCCATCT TCAGCTCCAT CCCTGTACAT GTGACTGGGC CAGCTGGACG ATTGTCTAAC 
TCTCACAGCA TGTCTGGTTT CACAGCTCCT GGAGGTACTG TAGGAGGTAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952487.1
CDS	90..3503
Translation	MKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTLLENYFSSLKNPKLREEQEAARRRQQREGKSNTTTPTKVHETKDSAAEEEKSAPVKKASPAKARRKLSKKGRKMAGRKRGRPKKLLTTTADRKSSKSQTALETLHPQNVLVPQSPTKSPPQDSYKTPHSPYYQLPPKVQRQPSNQLLSSPTPPALQKLLDSFRIQYLQLMSYMKTPHYKAGLQQLLEQEKEKNSHLLGAAQQLLTHCQAQKEEIKKLFQQKLDELGVKALTYSDLIQAQKEIFAHNQQLKEQSRQLEEENGALRNHSLQLLKARCEELKLDWNTLSLENLMKEKLALKNQISEKQKHCLELQISIVELEKFQRQQELLQLKSSTLPEEPPPSHNRTKGSLGNHFHAEQSSVRMMPPPSSHTNGLSPERSLNGHASHYELYHSCRGGSRPCSKESTPSHSEHADIVPSTQTHAAKHKGEKISGPTQPDYTRYSPAKIAMRRHLNQEPLPNGRSTEINHRCNVTKDHSFPSISPGIPNGVQFSPQESNPSSPELTHTPGERGPEKMFRERNSPSNPETITSLPISIPLSTVQPNKLPVSIPLASVVLPSRVEKRNSPSPGCHSRDGSLSENQYGATSNSLSYAAVSKPLATSGFFYPTWSSSLNGPVLNSHGSETDGMDDSSNSGTLSNSGGSRSSTPSHSHLISQRSPAQHSTSASPCTFPDPSKGSGEGDIDTSDAEMEAKRRIIFTITSGSSNGRSPSNKHSPLTATIRMECGTGQDAKRRSGRRKRSSAGSHNSAVSPKRRSLPTSVGLNSYTSGSPLNISSMVSNINQPLEITAISSPEASMKSSPASYQDHELPPVLKKEKPLSLSNGVHYSPLTTEDEAESEEDTQSTRTERKSSSLCFESKDSPQRSEGERGLPYSSKKSSCESVKWKATFSPISDATPGRASDSPSSPHRQNTHYSSGEGGRRGSGGLSPGSANGHSDHPRVEGGKGECNLFLSKKMSSEGSKREDRGPTAKLDRSSSKVPRSRERDGNTVFMTQGTGALMNGKGPGSNPLHPHHHFLNSLAAGSQLALQGGLNSVASNSVLQSIFSSIPVHVTGPAGRLSNSHSMSGFTAPGGTVGGI

Target ORF information:

RefSeq Version	XM_018096998.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096998.2

ORF Insert Sequence:

ATGAAGGAAG GTGGCAGAAT CGTATCCTCC AAACCATTTG CACCTCTGAA TTTCAGAATA 
AATAGCCGAA ACTTGAGTGA TATTGGCACT ATTATGAGAG TTGTGGAGTT ATCACCTCTT 
AAAGGATCTG TATCTTGGAC TGGGAAGCCT GTTTCTTATT ACCTGCACAC TATAGACCGG 
ACCTTACTTG AAAATTATTT TTCTAGTTTG AAAAATCCAA AACTCAGGGA GGAACAGGAA 
GCAGCAAGAC GCAGACAACA GAGAGAAGGG AAGAGCAATA CCACAACTCC TACAAAAGTT 
CATGAGACAA AGGACTCTGC TGCAGAGGAA GAAAAGTCTG CCCCAGTGAA AAAGGCTTCT 
CCAGCCAAAG CCCGAAGAAA GCTCAGCAAA AAGGGCAGAA AAATGGCAGG TCGCAAGCGG 
GGACGTCCAA AAAAGTTGCT TACCACAACA GCTGACCGCA AGTCCTCTAA ATCACAGACT 
GCACTGGAAA CCCTCCATCC ACAAAATGTC CTTGTTCCTC AGTCTCCAAC AAAATCACCC 
CCTCAGGATT CATACAAAAC ACCTCATAGT CCCTATTATC AGCTACCTCC AAAAGTGCAA 
CGTCAGCCTT CCAATCAGCT TCTGTCATCT CCTACCCCTC CAGCCCTACA GAAATTGCTC 
GATTCATTTA GGATCCAGTA TTTGCAACTT ATGTCTTACA TGAAAACACC CCATTACAAG 
GCTGGTCTGC AGCAACTGCT GGAACAGGAA AAGGAAAAGA ATTCCCACCT TCTTGGTGCT 
GCTCAGCAGC TGCTCACCCA CTGTCAGGCT CAGAAAGAAG AAATAAAGAA ACTTTTTCAG 
CAGAAACTGG ATGAGCTGGG TGTTAAGGCC CTCACTTACA GTGACCTCAT TCAAGCGCAG 
AAAGAGATTT TTGCTCACAA CCAGCAGCTG AAAGAGCAAA GTCGACAGTT AGAGGAAGAG 
AATGGAGCAT TAAGAAACCA CAGCTTGCAG CTGCTGAAGG CACGTTGTGA AGAATTAAAG 
CTGGACTGGA ATACACTGTC TTTGGAAAAT CTCATGAAGG AGAAGTTGGC CCTAAAAAAT 
CAGATATCCG AAAAGCAGAA GCACTGTTTG GAGCTACAAA TCAGCATTGT GGAACTAGAA 
AAGTTTCAGA GACAACAAGA GCTGCTCCAG CTCAAGTCTT CCACCTTGCC AGAGGAACCA 
CCACCCAGCC ATAATCGAAC CAAAGGTAGC CTTGGCAACC ACTTCCACGC TGAACAGTCT 
TCAGTGCGCA TGATGCCTCC TCCATCTTCT CACACAAATG GTCTAAGCCC TGAGCGGTCC 
TTGAATGGTC ATGCTTCTCA TTATGAGCTG TACCATTCCT GTCGAGGAGG AAGCAGGCCC 
TGCTCCAAGG AAAGTACACC ATCTCACTCA GAGCATGCCG ACATTGTGCC CAGTACCCAA 
ACCCATGCAG CCAAGCACAA AGGAGAGAAA ATATCTGGCC CAACCCAACC AGACTACACG 
CGCTACTCCC CTGCCAAAAT TGCAATGAGA CGGCACCTGA ACCAGGAACC CTTACCCAAT 
GGTAGAAGCA CTGAAATAAA TCATAGGTGT AATGTTACAA AAGACCACAG CTTCCCATCC 
ATAAGCCCTG GAATTCCAAA TGGTGTCCAA TTTAGCCCTC AGGAATCAAA TCCTTCTTCC 
CCTGAGTTAA CTCATACACC AGGGGAGAGA GGACCTGAAA AGATGTTTCG AGAGCGAAAT 
TCCCCTTCTA ATCCAGAAAC CATCACCAGT CTGCCTATTA GTATTCCCCT GAGCACAGTC 
CAGCCTAACA AGCTGCCAGT CAGTATTCCA CTGGCCAGTG TAGTCCTGCC AAGTCGTGTG 
GAAAAGAGAA ACAGCCCCAG TCCCGGATGT CACAGCAGAG ATGGCTCACT TTCAGAGAAT 
CAATATGGTG CTACTTCTAA TAGCCTCAGT TATGCTGCTG TCTCAAAACC ATTGGCTACC 
TCAGGTTTCT TCTATCCAAC CTGGTCTTCA TCTCTAAATG GACCAGTTTT GAATAGCCAT 
GGGTCAGAGA CGGATGGAAT GGATGACTCA TCAAATTCTG GCACATTGTC TAATTCAGGA 
GGATCACGGA GCTCCACCCC TTCCCACAGT CACCTGATCT CTCAGCGCTC CCCTGCCCAA 
CATTCCACAT CTGCTAGTCC TTGCACATTT CCAGATCCAA GCAAAGGTTC TGGTGAAGGG 
GATATTGACA CAAGTGATGC TGAAATGGAA GCAAAGCGTC GGATTATCTT CACCATTACC 
AGTGGAAGCT CCAACGGCAG GTCACCATCA AACAAACACA GCCCTTTGAC TGCCACAATC 
CGCATGGAGT GTGGCACAGG GCAGGATGCC AAAAGGAGAA GTGGTAGACG AAAAAGATCC 
TCTGCTGGCA GTCACAATTC AGCTGTATCA CCCAAACGCA GATCTCTTCC CACATCTGTT 
GGTCTAAACA GTTATACTTC AGGATCTCCA CTGAATATCA GCTCAATGGT GAGTAACATT 
AACCAGCCCT TGGAGATTAC TGCAATCTCT TCCCCTGAAG CCTCTATGAA GAGTTCCCCT 
GCTTCCTATC AGGATCATGA GTTGCCCCCA GTTTTAAAAA AGGAAAAACC CCTTTCTTTA 
TCTAATGGCG TTCATTATTC ACCCTTAACA ACAGAGGATG AGGCTGAGTC TGAGGAAGAC 
ACCCAGAGTA CAAGAACGGA GCGAAAGAGT TCCAGCCTGT GCTTTGAAAG CAAAGACTCC 
CCCCAGAGAA GTGAAGGGGA AAGAGGTCTC CCATACTCTA GCAAGAAGTC TTCATGTGAG 
AGTGTAAAAT GGAAAGCAAC ATTCTCTCCA ATTTCTGATG CAACTCCGGG TCGTGCCTCT 
GACAGCCCTT CCTCACCTCA CCGTCAGAAC ACACATTATT CATCTGGAGA GGGAGGCCGC 
AGAGGATCAG GGGGATTGAG TCCTGGCTCT GCAAATGGCC ACAGTGACCA TCCTAGGGTA 
GAAGGGGGCA AAGGAGAGTG CAACCTTTTC TTAAGTAAAA AGATGTCTTC TGAAGGTAGC 
AAAAGAGAAG ACAGGGGTCC TACAGCAAAA CTGGATCGTT CCAGCAGTAA AGTCCCTCGA 
AGCCGTGAAA GGGATGGGAA CACTGTGTTC ATGACACAAG GAACTGGTGC TCTCATGAAT 
GGCAAGGGAC CTGGCTCAAA TCCTTTACAC CCACACCATC ATTTTTTGAA CAGCTTGGCA 
GCTGGCTCTC AGCTTGCCTT GCAAGGTGGG CTTAACTCTG TTGCTAGCAA TTCTGTTCTG 
CAGTCCATCT TCAGCTCCAT CCCTGTACAT GTGACTGGGC CAGCTGGACG ATTGTCTAAC 
TCTCACAGCA TGTCTGGTTT CACAGCTCCT GGAGGTACTG TAGGAGGTAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dot1l ( XM_012954295.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809749.1 Xenopus tropicalis DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X1, mRNA.

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CloneID	OXa36930
Clone ID Related Accession (Same CDS sequence)	XM_012954295.2
Accession Version	XM_012954295.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012954295.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGAGA AGCTGGAACT AAGGCTGAAG TCCCCAGTGG GAGCCGAGCC TGCCGTCTAC 
CCTTGGCCGC TGCCTGTTTA CGATAAACAC CATGATGCTG CACATGAAAT TATTGAAACC 
ATCAGATGGG TCTGTGAAGA AATTCCAGAT CTCAAGCTTG CAATGGAAAA CTATGTTCTG 
ATAGACTATG ACACAAAGTG CTTTGAGAGC ATGCAGCGGC TGTGTGAGAA GTATAACAGA 
GCCATTGACA GCATCCATCA GCTGTGGAAA GGCACCACTC ATCCTATGAA GTTGAAAACT 
CGCCCTTCTA CTGGTCTCCT TCGCCACATT TTGCAGCAGG TCTACAACCA CTCTGTAACA 
GACCCTGAAA AACTCAACAA TTATGAGCCG TTTTCTCCTG AGGTCTATGG AGAAACCTCC 
TTTGATTTGG TTGCCCAGAT GATTGATGAA ATTAAGATGA CTGAGGATGA TCTCTTTGTG 
GACCTGGGAA GTGGTGTCGG GCAGGTTGTT CTGCAGGTTG CTGCAGCAAC AGGTTGCAAG 
CATCATTATG GTGTAGAGAA AGCGGATATC CCAGCCAAGT ATGCAGAGAC AATGGACACT 
GAGTTCAGGA AATGGATGAA GTGGTATGGA AAGAAGCATT CAGAGTACAC ACTGGAGCGT 
GGAGACTTCC TCTCAGAGGA GTGGAGGGAA AGAATAGCTA ACACAAGTGT TATTTTTGTG 
AATAATTTTG CCTTTGGTCC TGAGGTTGAC CACCAGCTAA AGGAGAGATT CGCCAACATG 
AAGGAAGGTG GCAGAATCGT ATCCTCCAAA CCATTTGCAC CTCTGAATTT CAGAATAAAT 
AGCCGAAACT TGAGTGATAT TGGCACTATT ATGAGAGTTG TGGAGTTATC ACCTCTTAAA 
GGATCTGTAT CTTGGACTGG GAAGCCTGTT TCTTATTACC TGCACACTAT AGACCGGACC 
TTACTTGAAA ATTATTTTTC TAGTTTGAAA AATCCAAAAC TCAGGGAGGA ACAGGAAGCA 
GCAAGACGCA GACAACAGAG AGAAGGGAAG AGCAATACCA CAACTCCTAC AAAAGTTCAT 
GAGACAAAGG ACTCTGCTGC AGAGGAAGAA AAGTCTGCCC CAGTGAAAAA GGCTTCTCCA 
GCCAAAGCCC GAAGAAAGCT CAGCAAAAAG GGCAGAAAAA TGGCAGGTCG CAAGCGGGGA 
CGTCCAAAAA AGTTGCTTAC CACAACAGCT GACCGCAAGT CCTCTAAATC ACAGACTGCA 
CTGGAAACCC TCCATCCACA AAATGTCCTT GTTCCTCAGT CTCCAACAAA ATCACCCCCT 
CAGGATTCAT ACAAAACACC TCATAGTCCC TATTATCAGC TACCTCCAAA AGTGCAACGT 
CAGCCTTCCA ATCAGCTTCT GTCATCTCCT ACCCCTCCAG CCCTACAGAA ATTGCTCGAT 
TCATTTAGGA TCCAGTATTT GCAACTTATG TCTTACATGA AAACACCCCA TTACAAGGCT 
GGTCTGCAGC AACTGCTGGA ACAGGAAAAG GAAAAGAATT CCCACCTTCT TGGTGCTGCT 
CAGCAGCTGC TCACCCACTG TCAGGCTCAG AAAGAAGAAA TAAAGAAACT TTTTCAGCAG 
AAACTGGATG AGCTGGGTGT TAAGGCCCTC ACTTACAGTG ACCTCATTCA AGCGCAGAAA 
GAGATTTTTG CTCACAACCA GCAGCTGAAA GAGCAAAGTC GACAGTTAGA GGAAGAGAAT 
GGAGCATTAA GAAACCACAG CTTGCAGCTG CTGAAGGCAC GTTGTGAAGA ATTAAAGCTG 
GACTGGAATA CACTGTCTTT GGAAAATCTC ATGAAGGAGA AGTTGGCCCT AAAAAATCAG 
ATATCCGAAA AGCAGAAGCA CTGTTTGGAG CTACAAATCA GCATTGTGGA ACTAGAAAAG 
TTTCAGAGAC AACAAGAGCT GCTCCAGCTC AAGTCTTCCA CCTTGCCAGA GGAACCACCA 
CCCAGCCATA ATCGAACCAA AGGTAGCCTT GGCAACCACT TCCACGCTGA ACAGTCTTCA 
GTGCGCATGA TGCCTCCTCC ATCTTCTCAC ACAAATGGTC TAAGCCCTGA GCGGTCCTTG 
AATGGTCATG CTTCTCATTA TGAGCTGTAC CATTCCTGTC GAGGAGGAAG CAGGCCCTGC 
TCCAAGGAAA GTACACCATC TCACTCAGAG CATGCCGACA TTGTGCCCAG TACCCAAACC 
CATGCAGCCA AGCACAAAGG AGAGAAAATA TCTGGCCCAA CCCAACCAGA CTACACGCGC 
TACTCCCCTG CCAAAATTGC AATGAGACGG CACCTGAACC AGGAACCCTT ACCCAATGGT 
AGAAGCACTG AAATAAATCA TAGGTGTAAT GTTACAAAAG ACCACAGCTT CCCATCCATA 
AGCCCTGGAA TTCCAAATGG TGTCCAATTT AGCCCTCAGG AATCAAATCC TTCTTCCCCT 
GAGTTAACTC ATACACCAGG GGAGAGAGGA CCTGAAAAGA TGTTTCGAGA GCGAAATTCC 
CCTTCTAATC CAGAAACCAT CACCAGTCTG CCTATTAGTA TTCCCCTGAG CACAGTCCAG 
CCTAACAAGC TGCCAGTCAG TATTCCACTG GCCAGTGTAG TCCTGCCAAG TCGTGTGGAA 
AAGAGAAACA GCCCCAGTCC CGGATGTCAC AGCAGAGATG GCTCACTTTC AGAGAATCAA 
TATGGTGCTA CTTCTAATAG CCTCAGTTAT GCTGCTGTCT CAAAACCATT GGCTACCTCA 
GGTTTCTTCT ATCCAACCTG GTCTTCATCT CTAAATGGAC CAGTTTTGAA TAGCCATGGG 
TCAGAGACGG ATGGAATGGA TGACTCATCA AATTCTGGCA CATTGTCTAA TTCAGGAGGA 
TCACGGAGCT CCACCCCTTC CCACAGTCAC CTGATCTCTC AGCGCTCCCC TGCCCAACAT 
TCCACATCTG CTAGTCCTTG CACATTTCCA GATCCAAGCA AAGGTTCTGG TGAAGGGGAT 
ATTGACACAA GTGATGCTGA AATGGAAGCA AAGCGTCGGA TTATCTTCAC CATTACCAGT 
GGAAGCTCCA ACGGCAGGTC ACCATCAAAC AAACACAGCC CTTTGACTGC CACAATCCGC 
ATGGAGTGTG GCACAGGGCA GGATGCCAAA AGGAGAAGTG GTAGACGAAA AAGATCCTCT 
GCTGGCAGTC ACAATTCAGC TGTATCACCC AAACGCAGAT CTCTTCCCAC ATCTGTTGGT 
CTAAACAGTT ATACTTCAGG ATCTCCACTG AATATCAGCT CAATGGTGAG TAACATTAAC 
CAGCCCTTGG AGATTACTGC AATCTCTTCC CCTGAAGCCT CTATGAAGAG TTCCCCTGCT 
TCCTATCAGG ATCATGAGTT GCCCCCAGTT TTAAAAAAGG AAAAACCCCT TTCTTTATCT 
AATGGCGTTC ATTATTCACC CTTAACAACA GAGGATGAGG CTGAGTCCGA GGAAGACACC 
CAGAGTACAA GAACGGAGCG AAAGAGTTCC AGCCTGTGCT TTGAAAGCAA AGACTCCCCC 
CAGAGAAGTG AAGGGGAAAG AGGTCTCCCA TACTCTAGCA AGAAGTCTTC ATGTGAGAGT 
GTAAAATGGA AAGCAACATT CTCTCCAATT TCTGATGCAA CTCCGGGTCG TGCCTCTGAC 
AGCCCTTCCT CACCTCACCG TCAGAACACA CATTATTCAT CTGGAGAGGG AGGCCGCAGA 
GGATCAGGGG GATTGAGTCC TGGCTCTGCA AATGGCCACA GTGACCATCC TAGGGTAGAA 
GGGGGCAAAG GAGAGTGCAA CCTTTTCTTA AGTAAAAAGA TGTCTTCTGA AGGTAGCAAA 
AGAGAAGACA GGGGTCCTAC AGCAAAACTG GATCGTTCCA GCAGTAAAGT CCCTCGAAGC 
CGTGAAAGGG ATGGGAACAC TGTGTTCATG ACACAAGGAA CTGGTGCTCT CATGAATGGC 
AAGGGACCTG GCTCAAATCC TTTACACCCA CACCATCATT TTTTGAACAG CTTGGCAGCT 
GGCTCTCAGC TTGCCTTGCA AGGTGGGCTT AACTCTGTTG CTAGCAATTC TGTTCTGCAG 
TCCATCTTCA GCTCCATCCC TGTACATGTG ACTGGGCCAG CTGGACGATT GTCTAACTCT 
CACAGCATGT CTGGTTTCAC AGCTCCTGGA GGTACTGTAG GAGGTATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809749.1
CDS	168..4358
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKCFESMQRLCEKYNRAIDSIHQLWKGTTHPMKLKTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATGCKHHYGVEKADIPAKYAETMDTEFRKWMKWYGKKHSEYTLERGDFLSEEWRERIANTSVIFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTLLENYFSSLKNPKLREEQEAARRRQQREGKSNTTTPTKVHETKDSAAEEEKSAPVKKASPAKARRKLSKKGRKMAGRKRGRPKKLLTTTADRKSSKSQTALETLHPQNVLVPQSPTKSPPQDSYKTPHSPYYQLPPKVQRQPSNQLLSSPTPPALQKLLDSFRIQYLQLMSYMKTPHYKAGLQQLLEQEKEKNSHLLGAAQQLLTHCQAQKEEIKKLFQQKLDELGVKALTYSDLIQAQKEIFAHNQQLKEQSRQLEEENGALRNHSLQLLKARCEELKLDWNTLSLENLMKEKLALKNQISEKQKHCLELQISIVELEKFQRQQELLQLKSSTLPEEPPPSHNRTKGSLGNHFHAEQSSVRMMPPPSSHTNGLSPERSLNGHASHYELYHSCRGGSRPCSKESTPSHSEHADIVPSTQTHAAKHKGEKISGPTQPDYTRYSPAKIAMRRHLNQEPLPNGRSTEINHRCNVTKDHSFPSISPGIPNGVQFSPQESNPSSPELTHTPGERGPEKMFRERNSPSNPETITSLPISIPLSTVQPNKLPVSIPLASVVLPSRVEKRNSPSPGCHSRDGSLSENQYGATSNSLSYAAVSKPLATSGFFYPTWSSSLNGPVLNSHGSETDGMDDSSNSGTLSNSGGSRSSTPSHSHLISQRSPAQHSTSASPCTFPDPSKGSGEGDIDTSDAEMEAKRRIIFTITSGSSNGRSPSNKHSPLTATIRMECGTGQDAKRRSGRRKRSSAGSHNSAVSPKRRSLPTSVGLNSYTSGSPLNISSMVSNINQPLEITAISSPEASMKSSPASYQDHELPPVLKKEKPLSLSNGVHYSPLTTEDEAESEEDTQSTRTERKSSSLCFESKDSPQRSEGERGLPYSSKKSSCESVKWKATFSPISDATPGRASDSPSSPHRQNTHYSSGEGGRRGSGGLSPGSANGHSDHPRVEGGKGECNLFLSKKMSSEGSKREDRGPTAKLDRSSSKVPRSRERDGNTVFMTQGTGALMNGKGPGSNPLHPHHHFLNSLAAGSQLALQGGLNSVASNSVLQSIFSSIPVHVTGPAGRLSNSHSMSGFTAPGGTVGGI

Target ORF information:

RefSeq Version	XM_012954295.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012954295.2

ORF Insert Sequence:

ATGGGCGAGA AGCTGGAACT AAGGCTGAAG TCCCCAGTGG GAGCCGAGCC TGCCGTCTAC 
CCTTGGCCGC TGCCTGTTTA CGATAAACAC CATGATGCTG CACATGAAAT TATTGAAACC 
ATCAGATGGG TCTGTGAAGA AATTCCAGAT CTCAAGCTTG CAATGGAAAA CTATGTTCTG 
ATAGACTATG ACACAAAGTG CTTTGAGAGC ATGCAGCGGC TGTGTGAGAA GTATAACAGA 
GCCATTGACA GCATCCATCA GCTGTGGAAA GGCACCACTC ATCCTATGAA GTTGAAAACT 
CGCCCTTCTA CTGGTCTCCT TCGCCACATT TTGCAGCAGG TCTACAACCA CTCTGTAACA 
GACCCTGAAA AACTCAACAA TTATGAGCCG TTTTCTCCTG AGGTCTATGG AGAAACCTCC 
TTTGATTTGG TTGCCCAGAT GATTGATGAA ATTAAGATGA CTGAGGATGA TCTCTTTGTG 
GACCTGGGAA GTGGTGTCGG GCAGGTTGTT CTGCAGGTTG CTGCAGCAAC AGGTTGCAAG 
CATCATTATG GTGTAGAGAA AGCGGATATC CCAGCCAAGT ATGCAGAGAC AATGGACACT 
GAGTTCAGGA AATGGATGAA GTGGTATGGA AAGAAGCATT CAGAGTACAC ACTGGAGCGT 
GGAGACTTCC TCTCAGAGGA GTGGAGGGAA AGAATAGCTA ACACAAGTGT TATTTTTGTG 
AATAATTTTG CCTTTGGTCC TGAGGTTGAC CACCAGCTAA AGGAGAGATT CGCCAACATG 
AAGGAAGGTG GCAGAATCGT ATCCTCCAAA CCATTTGCAC CTCTGAATTT CAGAATAAAT 
AGCCGAAACT TGAGTGATAT TGGCACTATT ATGAGAGTTG TGGAGTTATC ACCTCTTAAA 
GGATCTGTAT CTTGGACTGG GAAGCCTGTT TCTTATTACC TGCACACTAT AGACCGGACC 
TTACTTGAAA ATTATTTTTC TAGTTTGAAA AATCCAAAAC TCAGGGAGGA ACAGGAAGCA 
GCAAGACGCA GACAACAGAG AGAAGGGAAG AGCAATACCA CAACTCCTAC AAAAGTTCAT 
GAGACAAAGG ACTCTGCTGC AGAGGAAGAA AAGTCTGCCC CAGTGAAAAA GGCTTCTCCA 
GCCAAAGCCC GAAGAAAGCT CAGCAAAAAG GGCAGAAAAA TGGCAGGTCG CAAGCGGGGA 
CGTCCAAAAA AGTTGCTTAC CACAACAGCT GACCGCAAGT CCTCTAAATC ACAGACTGCA 
CTGGAAACCC TCCATCCACA AAATGTCCTT GTTCCTCAGT CTCCAACAAA ATCACCCCCT 
CAGGATTCAT ACAAAACACC TCATAGTCCC TATTATCAGC TACCTCCAAA AGTGCAACGT 
CAGCCTTCCA ATCAGCTTCT GTCATCTCCT ACCCCTCCAG CCCTACAGAA ATTGCTCGAT 
TCATTTAGGA TCCAGTATTT GCAACTTATG TCTTACATGA AAACACCCCA TTACAAGGCT 
GGTCTGCAGC AACTGCTGGA ACAGGAAAAG GAAAAGAATT CCCACCTTCT TGGTGCTGCT 
CAGCAGCTGC TCACCCACTG TCAGGCTCAG AAAGAAGAAA TAAAGAAACT TTTTCAGCAG 
AAACTGGATG AGCTGGGTGT TAAGGCCCTC ACTTACAGTG ACCTCATTCA AGCGCAGAAA 
GAGATTTTTG CTCACAACCA GCAGCTGAAA GAGCAAAGTC GACAGTTAGA GGAAGAGAAT 
GGAGCATTAA GAAACCACAG CTTGCAGCTG CTGAAGGCAC GTTGTGAAGA ATTAAAGCTG 
GACTGGAATA CACTGTCTTT GGAAAATCTC ATGAAGGAGA AGTTGGCCCT AAAAAATCAG 
ATATCCGAAA AGCAGAAGCA CTGTTTGGAG CTACAAATCA GCATTGTGGA ACTAGAAAAG 
TTTCAGAGAC AACAAGAGCT GCTCCAGCTC AAGTCTTCCA CCTTGCCAGA GGAACCACCA 
CCCAGCCATA ATCGAACCAA AGGTAGCCTT GGCAACCACT TCCACGCTGA ACAGTCTTCA 
GTGCGCATGA TGCCTCCTCC ATCTTCTCAC ACAAATGGTC TAAGCCCTGA GCGGTCCTTG 
AATGGTCATG CTTCTCATTA TGAGCTGTAC CATTCCTGTC GAGGAGGAAG CAGGCCCTGC 
TCCAAGGAAA GTACACCATC TCACTCAGAG CATGCCGACA TTGTGCCCAG TACCCAAACC 
CATGCAGCCA AGCACAAAGG AGAGAAAATA TCTGGCCCAA CCCAACCAGA CTACACGCGC 
TACTCCCCTG CCAAAATTGC AATGAGACGG CACCTGAACC AGGAACCCTT ACCCAATGGT 
AGAAGCACTG AAATAAATCA TAGGTGTAAT GTTACAAAAG ACCACAGCTT CCCATCCATA 
AGCCCTGGAA TTCCAAATGG TGTCCAATTT AGCCCTCAGG AATCAAATCC TTCTTCCCCT 
GAGTTAACTC ATACACCAGG GGAGAGAGGA CCTGAAAAGA TGTTTCGAGA GCGAAATTCC 
CCTTCTAATC CAGAAACCAT CACCAGTCTG CCTATTAGTA TTCCCCTGAG CACAGTCCAG 
CCTAACAAGC TGCCAGTCAG TATTCCACTG GCCAGTGTAG TCCTGCCAAG TCGTGTGGAA 
AAGAGAAACA GCCCCAGTCC CGGATGTCAC AGCAGAGATG GCTCACTTTC AGAGAATCAA 
TATGGTGCTA CTTCTAATAG CCTCAGTTAT GCTGCTGTCT CAAAACCATT GGCTACCTCA 
GGTTTCTTCT ATCCAACCTG GTCTTCATCT CTAAATGGAC CAGTTTTGAA TAGCCATGGG 
TCAGAGACGG ATGGAATGGA TGACTCATCA AATTCTGGCA CATTGTCTAA TTCAGGAGGA 
TCACGGAGCT CCACCCCTTC CCACAGTCAC CTGATCTCTC AGCGCTCCCC TGCCCAACAT 
TCCACATCTG CTAGTCCTTG CACATTTCCA GATCCAAGCA AAGGTTCTGG TGAAGGGGAT 
ATTGACACAA GTGATGCTGA AATGGAAGCA AAGCGTCGGA TTATCTTCAC CATTACCAGT 
GGAAGCTCCA ACGGCAGGTC ACCATCAAAC AAACACAGCC CTTTGACTGC CACAATCCGC 
ATGGAGTGTG GCACAGGGCA GGATGCCAAA AGGAGAAGTG GTAGACGAAA AAGATCCTCT 
GCTGGCAGTC ACAATTCAGC TGTATCACCC AAACGCAGAT CTCTTCCCAC ATCTGTTGGT 
CTAAACAGTT ATACTTCAGG ATCTCCACTG AATATCAGCT CAATGGTGAG TAACATTAAC 
CAGCCCTTGG AGATTACTGC AATCTCTTCC CCTGAAGCCT CTATGAAGAG TTCCCCTGCT 
TCCTATCAGG ATCATGAGTT GCCCCCAGTT TTAAAAAAGG AAAAACCCCT TTCTTTATCT 
AATGGCGTTC ATTATTCACC CTTAACAACA GAGGATGAGG CTGAGTCCGA GGAAGACACC 
CAGAGTACAA GAACGGAGCG AAAGAGTTCC AGCCTGTGCT TTGAAAGCAA AGACTCCCCC 
CAGAGAAGTG AAGGGGAAAG AGGTCTCCCA TACTCTAGCA AGAAGTCTTC ATGTGAGAGT 
GTAAAATGGA AAGCAACATT CTCTCCAATT TCTGATGCAA CTCCGGGTCG TGCCTCTGAC 
AGCCCTTCCT CACCTCACCG TCAGAACACA CATTATTCAT CTGGAGAGGG AGGCCGCAGA 
GGATCAGGGG GATTGAGTCC TGGCTCTGCA AATGGCCACA GTGACCATCC TAGGGTAGAA 
GGGGGCAAAG GAGAGTGCAA CCTTTTCTTA AGTAAAAAGA TGTCTTCTGA AGGTAGCAAA 
AGAGAAGACA GGGGTCCTAC AGCAAAACTG GATCGTTCCA GCAGTAAAGT CCCTCGAAGC 
CGTGAAAGGG ATGGGAACAC TGTGTTCATG ACACAAGGAA CTGGTGCTCT CATGAATGGC 
AAGGGACCTG GCTCAAATCC TTTACACCCA CACCATCATT TTTTGAACAG CTTGGCAGCT 
GGCTCTCAGC TTGCCTTGCA AGGTGGGCTT AACTCTGTTG CTAGCAATTC TGTTCTGCAG 
TCCATCTTCA GCTCCATCCC TGTACATGTG ACTGGGCCAG CTGGACGATT GTCTAACTCT 
CACAGCATGT CTGGTTTCAC AGCTCCTGGA GGTACTGTAG GAGGTATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

dot1l cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報