mast1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following mast1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mast1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa41025 XM_018089902.1
Latest version!
Xenopus tropicalis microtubule associated serine/threonine kinase 1 (mast1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OXa55394 XM_031899443.1
Latest version!
Xenopus tropicalis microtubule associated serine/threonine kinase 1 (mast1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa55395 XM_031899444.1
Latest version!
Xenopus tropicalis microtubule associated serine/threonine kinase 1 (mast1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa55396 XM_031899445.1
Latest version!
Xenopus tropicalis microtubule associated serine/threonine kinase 1 (mast1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa55397 XM_031899446.1
Latest version!
Xenopus tropicalis microtubule associated serine/threonine kinase 1 (mast1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa55398 XM_031899447.1
Latest version!
Xenopus tropicalis microtubule associated serine/threonine kinase 1 (mast1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa55399 XM_031899448.1
Latest version!
Xenopus tropicalis microtubule associated serine/threonine kinase 1 (mast1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa55400 XM_031899449.1
Latest version!
Xenopus tropicalis microtubule associated serine/threonine kinase 1 (mast1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa41025
    Clone ID Related Accession (Same CDS sequence) XM_018089902.1
    Accession Version XM_018089902.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5457bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product microtubule-associated serine/threonine-protein kinase 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683492.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGATTCACC CAGCGTGGAA CTGGGCCCAG ATCCGATCCC CCCTAGGGCG TAGCCGCGAT 
    CCGCCGTCTC CCTCCCCCTC GACGCGCCGC AGTCCTCCTC CTCCTCCTCA GGAACGCCTA
    CACCAGCTCC CCTTCCAGCC CACACTGGAT GAGCTCCACT TCCTTTCCAA ACACTTTGGC
    AGCACAGAAA GTATCCCCGA TGATGATGGG GGGCGCAGGT CACCTGCGGT CAGACCACGA
    TCCCGCAGCT TAAGTCCTGG CCGATCTCCC TCCTCTTATG ACAATGAAAT TGTGATGATG
    AACCATGTTT ATAAAGAGAG GTTTCCCAAG GCCACAGCGC AGATGGAGGA TAGGCTACGG
    ATGTTCATCT CCTCCTTCTC CCCCGAGAAT GTCCTACCAC TGGCGGACGG ACTCCTCAAC
    TTTATCCACC ATCAGATCGT GGAGCTGGCG AGGGACTGCC TGAACAGGAG CACCGAGGGC
    ATGATCACAA CCGCCCACTT TTACCAACTA CAGGAGAATC TGGAGAAGCT GCTGAATGAT
    GCAGTGGAAC GTTCTTCAGA GGGATCAGAA GTTGCCTTCA TTGCTCAACT GATGAAAAAT
    CTTTTAATCA TCATCTCTCG ACCTGCTCGT CTGCTGGAGT GTCTGGAATT TAACCCTGAA
    GAGTTCTATC ACATGCTGGA GGTAGCCGAG GACCAGGTGA AAGAAGGAAA CTTGAAAAAA
    GACATACCCC AATACATCCT GAGACAGTTG GGATTAACCA GGGATTCCTT CCCAGAGGTA
    GCCGCTCCCG AGGAACCAGT GACCCTGGAA GCACAAACCT CAGAAGCAGA GGACAACGTA
    GAGGCAAAGA GTGGGGCGGC CCATAAACCC ATCAGACCAC CCGAGGAAAG TGACTTTGAA
    AATATAAAGC TCATCAGTAA CGGAGCCTAT GGAGCGGTGT ATTTGGTGCG TCACCGGGAG
    ACGCGGCAAC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACTTGCGTCT GAGGAAACAA
    GTCCACCAGG CCTTTGTAGA ACGGGACATT CTGACCTTTG CTCAGAACCC CTTTGTGGTC
    AGCATGTTCT GCTCCTTCCA GACCCGTAGG CACCTCTGTA TGGTGATGGA GTATGTGGAA
    GGGGGTGACT GTGCCACCCT ACTGAAGCAC ATTGGTGCTC TCCCATTGGA AATGGCGCGG
    TTATATTTCG CAGAGACGGT TCTAGCTCTG GAATACCTAC ATAATTATGG GATTGTGCAC
    CGAGATCTGA AACCGGACAA TCTCCTAATC ACTTCGATGG GTCACATAAA GCTTACAGAT
    TTCGGCCTGT CCAAGATGGG GCTTATGAAC CTCACCACTA ATCTCTATGA AGGACACATC
    GAGAGCGAAG CAAGGGAATT CCTGGACAAG CAGGTCTGTG GAACCCCTGA ATACATCGCA
    CCGGAGGTTA TCCTTAGACA AGGTTACGGG AAGCCAGTGG ACTGGTGGGC GATGGGCATA
    ATTCTGTATG AGTTCCTGGT TGGTTGCGTG CCATTTTTTG GAGACACCCC AGAGGAGCTG
    TTTGGTCAGG TGATCAGTGA TGACATTGTG TGGCCAGAAG CAGAGGAAGC TCTACCCGCC
    GATGCCCAAA ATCTCATCTC CTGCCTCCTG CAGACAGATC CCTTACAAAG GCTTGGAACA
    GGGGGAGCCA TTGAGGTGAA AATTCACAGA TTTTTCACTG ATTTGGACTG GAATGGATTG
    CTCAGGCAGA AAGCTGAATT CATCCCTCAC TTGGAGGCAG AAGATGATAC CAGTTACTTT
    GACACTCGAT CGGATCGGTA CCAGCACATT AATTCCTACG ATGAGGATGA TACCAATGAT
    GAAGACTCGG TGGAGATCAG GCAGTTCTCA TCCTGCACCC CAAGATTTAG TAAGGTGTAT
    AGTAGTATGG AGCAGCTCTC CCAGCTGGAG CAGAAACCCC CCACTCCTCC TAGAGACACC
    AACAAACAAG AAAGGTCTGA GAAGACGACC AAGAGAGAGA CGTTGGGGGG CGTCACCCTT
    CGAGACCGCT GCTGGAGGAC CGTGTCTCCT GAAATGAAAC AATTCTCCGA TGCGGCAGAC
    GCAGAATCCA GCCCCCCCTT GGCTGCCCGG CGGCGCTTTT CTGCCTTAGT GGACCCGACC
    CGCTATACGT CTCCCCTGGA GTATGAGGTG GAGTACAGAG TGAGGGGAAG CAGCGGGCCG
    CTGCCCGCCC AAAGCAAGAT CCCTCTGGGA GAGCTGGAGC CAGACAAGTC TGTCGACTCT
    CAGCCCCACC AGGAGGCGGA TTCCTCTCCT CGCGCCACAA ACGAGATTGT CCTGCGCAGA
    GCCAGGCACC AACACCTGTC CGGGGACACC GAGAGGAGGA GAACCGGCGG GAAAGTCATC
    AAGTCGGCAT CGACCACCGC CCTGTCTGTC ATGATCCCTA CAGTTGAACA GCACAGCAGC
    TCTCCCCTGG TCAGCCCCAT GTCCCCCCGC TCTCTGTCGT CCAATCCCTC CTCCCGGGAT
    TCCTCTCCGA GCCGCGACTA CACGCCGGCA GTGAGCAGCC TCCGATCCCC CATTTGCATC
    CAACGCTCCG GGAAAAAATA CGGTTTCACC TTGCGGGCCA TCCGGGTGTA CATGGGGGAA
    AGTGATGTGT ACAGCGTGCA GCACATTGTC TGGCACGTGG AGGAAGGAGG GCCTGCCCAT
    GAAGCGGGTC TCTGTGCCGG GGACCTCATT ACACACATTA ACGGGGAGCC GGTGCACGGC
    CTGGTGCACA CTGAAGTTGT GGAGCTGATT TTAAAGAGCG GTAACAAGGT GTTAGTAACC
    ACCACCCCCT TTGAGAACAC CTCGATCCGA ACCGGCCCAG CCAGGAGAAG CAGTTATCGA
    TCCAAAATGG CGCGGAGGAA CAAGCGGATC GCTGGGAGAG AGGGGCAGGA GAGTAAGAAG
    CGGAGCTCTC TATTCCGTAA GATCACCAAG CAATCCAATC TTCTGCACAC CAGCCGTTCC
    CTCTCCTCTC TGAGCCGCTC CCTCTCCTCA AGTGACAGTC TTCCAGGATC ACCCACTCAC
    AGCCTCTCGG CCCGTTCCCC GACCCATAGC TACCGCTCCA TGGACTCTGC TTACCTTGGT
    GCTTCTTCGC AGGGCAGTTC CCCCTCTTCC AGTACCCCTA ATTCCCCTGC ACCACCACCT
    CCTGCCTCAG CCTCTTCCTG TGCGTCAGCC TCCTCCTCCT CCACCACTCC TGCTCCCCCC
    TCCCCACACA TCCGACCCAG CACCCTGCAT GGCTTATCTC CCAAACTGCA CCGCCAGTAT
    CGCTCAGCGC GATGTAAATC TGCGGGTAAT ATGCCCCTCT CTCCCCTTGC ACACACCCCT
    TCCCCTACTG CAGCTGCCTC ACCCCCTCTC CTACCAAGTC ACAGCATTGG CAGCAGCCTG
    ACCACTCAGA GCTTTCCCCC AAAACTGCAT CCATCTCCAC CAGTGTCACG CCCTCGCCCA
    AAGAGCGCAG AGCCTCCACG GTCCCCACTT CTTAAGAGAG TGCAGTCTGC AGAGAAACTG
    GGTGGGGGAG GAGGTGGTGG GTCACCCAGA AAACAGGAGC CGGGAGGGGG AGGAACAATA
    GAGGGAGATT TAGCAGAAGC CATTGGTGTG AGGAGCCAGC CAGTAAGGAG AGTGGGTAGG
    CAGGAGTCTC CTCGCAGTCT TTGTGGAGGG GAAAATATGA TAGGAGAGAA CTTTGAGAGG
    CTGGAAGGAG AAGGAGCGAT AGGACTATCG CCGCTAGGAG ACAGTCTGAA ATGTAGTGAG
    GGTAAAGTCC AAGCAGCCGT TAAGGTTGGA GTAGGATCAA GGACTACTCC CCCATTTTCT
    CCCCAAGAAC CTCCAGCCGC ATCAACTGCA GTCCTTCCCC CCCAAAGTGA CCCTTCCCTT
    GCTATGGTAG GAAGGTGCCA AGTGAAAACC CTAAGTCCTG TACAAGAACA TGAAGGTAGA
    AGGGGGGAAG GAGAGGACGG GGGAGCAGAG GGAGAGGGAA GAAAGGGAGA TTATGAGGAG
    GAACAAGGAA GAACAAGAGA ACAATGGTAC TTAGAGGTGG TAGAAGAGTT GACTACAGTA
    GGGAAAAGCA GCATGCATGC TGGGAGTAAA GCGACTCCCT CTAACCTCAC AGCCGACTCT
    AGTATCTACC ATTCCCACTC AAATAAGAGT AAAGTCGCTG TGGTTGACTC TATGAAGAGC
    ACACCCTCTC ACCCTACAGG TACAACTCTG GAGCAGTCTC TTTCTTTGAA AGGGATTAGA
    TCTGATGCCG ACTCTAAAAA GGGGCAAGAT CCTAAAAACT GCCAGATTAC TCCTCTTCTT
    CCCGTAAGGG AGGGCCTAAA TGTTGAACCA ATTCAAAATG GTTCTACTCA CCCTCCTGAA
    CTTTCCCACG TGGCCAAGGA GCAAAGTATC GACCATCTAA AAGGTCCATC ATCTTCTGGC
    CAACAAATGA AAAGCACTAG CACTTTCCTA CCTACTCAGC TTCACCCAAC CACTGGTAGA
    AAGCTGGAGG CTGATAATGT AAGTAGGCAT TTACTCTGTA CAGAGGGTAC AAAGTATGGT
    CTGATTAAAC CAACAGATTC TCTGTCTATT AGTATTAAAG GGGAGAAGGC CAGTTTAGCA
    AAAAGCCCCC AGGATGATCC AGTAAAGGCC GCTGATCATA CTACTGGGGT GCAAGGACCT
    GCTCCTGCAA AAGGACCAGC AATTTCACCT GTAAATATTA AAATCGGCAG AGAAGGATCT
    GAACAGACAA GGGTGACCAC TACTGACCCA ACTATTAATA GGCTGAGCGA AACACAAACT
    AATCAGAGAC CTGCAGCTGC CACTGGTCTT GCTAGCAATA TGGTACTTAG GGAGATGGAG
    AAGAAGGAAG AGTTCAGTTC AGCCCAGAAT ATAAAGAAAC CAAGTGGAGC AGAATTAGAA
    CAGAAGCGGG TGGCCATTGT AGTCCCGATT GTAACTAGAC CCAGTGATGT AAAGGCTGAC
    ACAACAAGGG TAGTATCTGC TCCCCTGACT GTTAATAGAC CTGCCGCAGG AGAGAGTGAC
    CAAGCAAAAG TGACCACTGT TACCCCAACT ATTGATAAAG TCAGTGGCCC AGAAAGTGCC
    TCTGCCAAGG TGACCGCCGT TGCCTCAATG AGTAGCGCTA ACAAAGCTGT TAATGAACCA
    CCAAGCCTGT TCCCCGATAA TAAAGTAGCC GACTCCAATC AAATAAGGTT CTCGTCACAG
    AATAACAAGG GCGCGAGAGA ATCAGAAAGT GACCAGATAA GGGCAACGGG CAATATTCCC
    GTTATTAACC AGACCGCTCA AACAACGGTT CCTTCCCCAA CACCCACAAA TGGCCAAGGA
    CCCCCTATAA TTATCCGAGT TAGTGGGGTT CCATGTGATG CCCAGGGCGG GCTGTGTCCT
    GAGAAACCTT CCAGCCCCCA AAGAGCGCCC ACCACTACCA GCCAACCCCC TTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017945391.1
    CDS1..5457
    Translation

    Target ORF information:

    RefSeq Version XM_018089902.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis microtubule associated serine/threonine kinase 1 (mast1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018089902.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGATTCACC CAGCGTGGAA CTGGGCCCAG ATCCGATCCC CCCTAGGGCG TAGCCGCGAT 
    CCGCCGTCTC CCTCCCCCTC GACGCGCCGC AGTCCTCCTC CTCCTCCTCA GGAACGCCTA
    CACCAGCTCC CCTTCCAGCC CACACTGGAT GAGCTCCACT TCCTTTCCAA ACACTTTGGC
    AGCACAGAAA GTATCCCCGA TGATGATGGG GGGCGCAGGT CACCTGCGGT CAGACCACGA
    TCCCGCAGCT TAAGTCCTGG CCGATCTCCC TCCTCTTATG ACAATGAAAT TGTGATGATG
    AACCATGTTT ATAAAGAGAG GTTTCCCAAG GCCACAGCGC AGATGGAGGA TAGGCTACGG
    ATGTTCATCT CCTCCTTCTC CCCCGAGAAT GTCCTACCAC TGGCGGACGG ACTCCTCAAC
    TTTATCCACC ATCAGATCGT GGAGCTGGCG AGGGACTGCC TGAACAGGAG CACCGAGGGC
    ATGATCACAA CCGCCCACTT TTACCAACTA CAGGAGAATC TGGAGAAGCT GCTGAATGAT
    GCAGTGGAAC GTTCTTCAGA GGGATCAGAA GTTGCCTTCA TTGCTCAACT GATGAAAAAT
    CTTTTAATCA TCATCTCTCG ACCTGCTCGT CTGCTGGAGT GTCTGGAATT TAACCCTGAA
    GAGTTCTATC ACATGCTGGA GGTAGCCGAG GACCAGGTGA AAGAAGGAAA CTTGAAAAAA
    GACATACCCC AATACATCCT GAGACAGTTG GGATTAACCA GGGATTCCTT CCCAGAGGTA
    GCCGCTCCCG AGGAACCAGT GACCCTGGAA GCACAAACCT CAGAAGCAGA GGACAACGTA
    GAGGCAAAGA GTGGGGCGGC CCATAAACCC ATCAGACCAC CCGAGGAAAG TGACTTTGAA
    AATATAAAGC TCATCAGTAA CGGAGCCTAT GGAGCGGTGT ATTTGGTGCG TCACCGGGAG
    ACGCGGCAAC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACTTGCGTCT GAGGAAACAA
    GTCCACCAGG CCTTTGTAGA ACGGGACATT CTGACCTTTG CTCAGAACCC CTTTGTGGTC
    AGCATGTTCT GCTCCTTCCA GACCCGTAGG CACCTCTGTA TGGTGATGGA GTATGTGGAA
    GGGGGTGACT GTGCCACCCT ACTGAAGCAC ATTGGTGCTC TCCCATTGGA AATGGCGCGG
    TTATATTTCG CAGAGACGGT TCTAGCTCTG GAATACCTAC ATAATTATGG GATTGTGCAC
    CGAGATCTGA AACCGGACAA TCTCCTAATC ACTTCGATGG GTCACATAAA GCTTACAGAT
    TTCGGCCTGT CCAAGATGGG GCTTATGAAC CTCACCACTA ATCTCTATGA AGGACACATC
    GAGAGCGAAG CAAGGGAATT CCTGGACAAG CAGGTCTGTG GAACCCCTGA ATACATCGCA
    CCGGAGGTTA TCCTTAGACA AGGTTACGGG AAGCCAGTGG ACTGGTGGGC GATGGGCATA
    ATTCTGTATG AGTTCCTGGT TGGTTGCGTG CCATTTTTTG GAGACACCCC AGAGGAGCTG
    TTTGGTCAGG TGATCAGTGA TGACATTGTG TGGCCAGAAG CAGAGGAAGC TCTACCCGCC
    GATGCCCAAA ATCTCATCTC CTGCCTCCTG CAGACAGATC CCTTACAAAG GCTTGGAACA
    GGGGGAGCCA TTGAGGTGAA AATTCACAGA TTTTTCACTG ATTTGGACTG GAATGGATTG
    CTCAGGCAGA AAGCTGAATT CATCCCTCAC TTGGAGGCAG AAGATGATAC CAGTTACTTT
    GACACTCGAT CGGATCGGTA CCAGCACATT AATTCCTACG ATGAGGATGA TACCAATGAT
    GAAGACTCGG TGGAGATCAG GCAGTTCTCA TCCTGCACCC CAAGATTTAG TAAGGTGTAT
    AGTAGTATGG AGCAGCTCTC CCAGCTGGAG CAGAAACCCC CCACTCCTCC TAGAGACACC
    AACAAACAAG AAAGGTCTGA GAAGACGACC AAGAGAGAGA CGTTGGGGGG CGTCACCCTT
    CGAGACCGCT GCTGGAGGAC CGTGTCTCCT GAAATGAAAC AATTCTCCGA TGCGGCAGAC
    GCAGAATCCA GCCCCCCCTT GGCTGCCCGG CGGCGCTTTT CTGCCTTAGT GGACCCGACC
    CGCTATACGT CTCCCCTGGA GTATGAGGTG GAGTACAGAG TGAGGGGAAG CAGCGGGCCG
    CTGCCCGCCC AAAGCAAGAT CCCTCTGGGA GAGCTGGAGC CAGACAAGTC TGTCGACTCT
    CAGCCCCACC AGGAGGCGGA TTCCTCTCCT CGCGCCACAA ACGAGATTGT CCTGCGCAGA
    GCCAGGCACC AACACCTGTC CGGGGACACC GAGAGGAGGA GAACCGGCGG GAAAGTCATC
    AAGTCGGCAT CGACCACCGC CCTGTCTGTC ATGATCCCTA CAGTTGAACA GCACAGCAGC
    TCTCCCCTGG TCAGCCCCAT GTCCCCCCGC TCTCTGTCGT CCAATCCCTC CTCCCGGGAT
    TCCTCTCCGA GCCGCGACTA CACGCCGGCA GTGAGCAGCC TCCGATCCCC CATTTGCATC
    CAACGCTCCG GGAAAAAATA CGGTTTCACC TTGCGGGCCA TCCGGGTGTA CATGGGGGAA
    AGTGATGTGT ACAGCGTGCA GCACATTGTC TGGCACGTGG AGGAAGGAGG GCCTGCCCAT
    GAAGCGGGTC TCTGTGCCGG GGACCTCATT ACACACATTA ACGGGGAGCC GGTGCACGGC
    CTGGTGCACA CTGAAGTTGT GGAGCTGATT TTAAAGAGCG GTAACAAGGT GTTAGTAACC
    ACCACCCCCT TTGAGAACAC CTCGATCCGA ACCGGCCCAG CCAGGAGAAG CAGTTATCGA
    TCCAAAATGG CGCGGAGGAA CAAGCGGATC GCTGGGAGAG AGGGGCAGGA GAGTAAGAAG
    CGGAGCTCTC TATTCCGTAA GATCACCAAG CAATCCAATC TTCTGCACAC CAGCCGTTCC
    CTCTCCTCTC TGAGCCGCTC CCTCTCCTCA AGTGACAGTC TTCCAGGATC ACCCACTCAC
    AGCCTCTCGG CCCGTTCCCC GACCCATAGC TACCGCTCCA TGGACTCTGC TTACCTTGGT
    GCTTCTTCGC AGGGCAGTTC CCCCTCTTCC AGTACCCCTA ATTCCCCTGC ACCACCACCT
    CCTGCCTCAG CCTCTTCCTG TGCGTCAGCC TCCTCCTCCT CCACCACTCC TGCTCCCCCC
    TCCCCACACA TCCGACCCAG CACCCTGCAT GGCTTATCTC CCAAACTGCA CCGCCAGTAT
    CGCTCAGCGC GATGTAAATC TGCGGGTAAT ATGCCCCTCT CTCCCCTTGC ACACACCCCT
    TCCCCTACTG CAGCTGCCTC ACCCCCTCTC CTACCAAGTC ACAGCATTGG CAGCAGCCTG
    ACCACTCAGA GCTTTCCCCC AAAACTGCAT CCATCTCCAC CAGTGTCACG CCCTCGCCCA
    AAGAGCGCAG AGCCTCCACG GTCCCCACTT CTTAAGAGAG TGCAGTCTGC AGAGAAACTG
    GGTGGGGGAG GAGGTGGTGG GTCACCCAGA AAACAGGAGC CGGGAGGGGG AGGAACAATA
    GAGGGAGATT TAGCAGAAGC CATTGGTGTG AGGAGCCAGC CAGTAAGGAG AGTGGGTAGG
    CAGGAGTCTC CTCGCAGTCT TTGTGGAGGG GAAAATATGA TAGGAGAGAA CTTTGAGAGG
    CTGGAAGGAG AAGGAGCGAT AGGACTATCG CCGCTAGGAG ACAGTCTGAA ATGTAGTGAG
    GGTAAAGTCC AAGCAGCCGT TAAGGTTGGA GTAGGATCAA GGACTACTCC CCCATTTTCT
    CCCCAAGAAC CTCCAGCCGC ATCAACTGCA GTCCTTCCCC CCCAAAGTGA CCCTTCCCTT
    GCTATGGTAG GAAGGTGCCA AGTGAAAACC CTAAGTCCTG TACAAGAACA TGAAGGTAGA
    AGGGGGGAAG GAGAGGACGG GGGAGCAGAG GGAGAGGGAA GAAAGGGAGA TTATGAGGAG
    GAACAAGGAA GAACAAGAGA ACAATGGTAC TTAGAGGTGG TAGAAGAGTT GACTACAGTA
    GGGAAAAGCA GCATGCATGC TGGGAGTAAA GCGACTCCCT CTAACCTCAC AGCCGACTCT
    AGTATCTACC ATTCCCACTC AAATAAGAGT AAAGTCGCTG TGGTTGACTC TATGAAGAGC
    ACACCCTCTC ACCCTACAGG TACAACTCTG GAGCAGTCTC TTTCTTTGAA AGGGATTAGA
    TCTGATGCCG ACTCTAAAAA GGGGCAAGAT CCTAAAAACT GCCAGATTAC TCCTCTTCTT
    CCCGTAAGGG AGGGCCTAAA TGTTGAACCA ATTCAAAATG GTTCTACTCA CCCTCCTGAA
    CTTTCCCACG TGGCCAAGGA GCAAAGTATC GACCATCTAA AAGGTCCATC ATCTTCTGGC
    CAACAAATGA AAAGCACTAG CACTTTCCTA CCTACTCAGC TTCACCCAAC CACTGGTAGA
    AAGCTGGAGG CTGATAATGT AAGTAGGCAT TTACTCTGTA CAGAGGGTAC AAAGTATGGT
    CTGATTAAAC CAACAGATTC TCTGTCTATT AGTATTAAAG GGGAGAAGGC CAGTTTAGCA
    AAAAGCCCCC AGGATGATCC AGTAAAGGCC GCTGATCATA CTACTGGGGT GCAAGGACCT
    GCTCCTGCAA AAGGACCAGC AATTTCACCT GTAAATATTA AAATCGGCAG AGAAGGATCT
    GAACAGACAA GGGTGACCAC TACTGACCCA ACTATTAATA GGCTGAGCGA AACACAAACT
    AATCAGAGAC CTGCAGCTGC CACTGGTCTT GCTAGCAATA TGGTACTTAG GGAGATGGAG
    AAGAAGGAAG AGTTCAGTTC AGCCCAGAAT ATAAAGAAAC CAAGTGGAGC AGAATTAGAA
    CAGAAGCGGG TGGCCATTGT AGTCCCGATT GTAACTAGAC CCAGTGATGT AAAGGCTGAC
    ACAACAAGGG TAGTATCTGC TCCCCTGACT GTTAATAGAC CTGCCGCAGG AGAGAGTGAC
    CAAGCAAAAG TGACCACTGT TACCCCAACT ATTGATAAAG TCAGTGGCCC AGAAAGTGCC
    TCTGCCAAGG TGACCGCCGT TGCCTCAATG AGTAGCGCTA ACAAAGCTGT TAATGAACCA
    CCAAGCCTGT TCCCCGATAA TAAAGTAGCC GACTCCAATC AAATAAGGTT CTCGTCACAG
    AATAACAAGG GCGCGAGAGA ATCAGAAAGT GACCAGATAA GGGCAACGGG CAATATTCCC
    GTTATTAACC AGACCGCTCA AACAACGGTT CCTTCCCCAA CACCCACAAA TGGCCAAGGA
    CCCCCTATAA TTATCCGAGT TAGTGGGGTT CCATGTGATG CCCAGGGCGG GCTGTGTCCT
    GAGAAACCTT CCAGCCCCCA AAGAGCGCCC ACCACTACCA GCCAACCCCC TTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55394
    Clone ID Related Accession (Same CDS sequence) XM_031899443.1
    Accession Version XM_031899443.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product microtubule-associated serine/threonine-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGATGATT CTGGCATTAT CAGACGCAGG AGGCTGCAGA AGGATCTGCC CCTGCCGAGG 
    AAGAGCAGCG GTTGCAGAAG CAGCAACAGA AAGAGTTTAA TCCTCACAAG CAGCAGTTCC
    CCCACTCTCC CCCGGCCCCA CTCCCCCCTG CCGGGGCATA TAGGTGGGAG CCCTATGGAC
    AGTCCCAGGA ACTTCTCCCC CAGCGCCGCT GCTCACTTCT CCTTTGCATC ATCTCGCAGC
    AGAACCGATG GCCGTCGCTG GTCCTTGGCC TCCCTCCCGT CGTCCGGCTA TGGGACAAAT
    ACCCCGAGTT CCACGGTGTC GTCCTCCTCC TCCTCTCAGG AACGCCTACA CCAGCTCCCC
    TTCCAGCCCA CACTGGATGA GCTCCACTTC CTTTCCAAAC ACTTTGGCAG CACAGAAAGT
    ATCCCCGATG ATGATGGGGG GCGCAGGTCA CCTGCGGTCA GACCACGATC CCGCAGCTTA
    AGTCCTGGCC GATCTCCCTC CTCTTATGAC AATGAAATTG TGATGATGAA CCATGTTTAT
    AAAGAGAGGT TTCCCAAGGC CACAGCGCAG ATGGAGGATA GGCTACGGAT GTTCATCTCC
    TCCTTCTCCC CCGAGAATGT CCTACCACTG GCGGACGGAC TCCTCAACTT TATCCACCAT
    CAGATCGTGG AGCTGGCGAG GGACTGCCTG AACAGGAGCA CCGAGGGCAT GATCACAACC
    GCCCACTTTT ACCAACTACA GGAGAATCTG GAGAAGCTGC TGAATGATGC AGTGGAACGT
    TCTTCAGAGG GATCAGAAGT TGCCTTCATT GCTCAACTGA TGAAAAATCT TTTAATCATC
    ATCTCTCGAC CTGCTCGTCT GCTGGAGTGT CTGGAATTTA ACCCTGAAGA GTTCTATCAC
    ATGCTGGAGG TAGCCGAGGA CCAGGTGAAA GAAGGAAACT TGAAAAAAGA CATACCCCAA
    TACATCCTGA GACAGTTGGG ATTAACCAGG GATTCCTTCC CAGAGGTAGC CGCTCCCGAG
    GAACCAGTGA CCCTGGAAGC ACAAACCTCA GAAGCAGAGG ACAACGTAGA GGCAAAGAGT
    GGGGCGGCCC ATAAACCCAT CAGACCACCC GAGGAAAGTG ACTTTGAAAA TATAAAGCTC
    ATCAGTAACG GAGCCTATGG AGCGGTGTAT TTGGTGCGTC ACCGGGAGAC GCGGCAACGC
    TTTGCCATGA AGAAGATCAA CAAGCAGAAC TTGCGTCTGA GGAAACAAGT CCACCAGGCC
    TTTGTAGAAC GGGACATTCT GACCTTTGCT CAGAACCCCT TTGTGGTCAG CATGTTCTGC
    TCCTTCCAGA CCCGTAGGCA CCTCTGTATG GTGATGGAGT ATGTGGAAGG GGGTGACTGT
    GCCACCCTAC TGAAGCACAT TGGTGCTCTC CCATTGGAAA TGGCGCGGTT ATATTTCGCA
    GAGACGGTTC TAGCTCTGGA ATACCTACAT AATTATGGGA TTGTGCACCG AGATCTGAAA
    CCGGACAATC TCCTAATCAC TTCGATGGGT CACATAAAGC TTACAGATTT CGGCCTGTCC
    AAGATGGGGC TTATGAACCT CACCACTAAT CTCTATGAAG GACACATCGA GAGCGAAGCA
    AGGGAATTCC TGGACAAGCA GGTCTGTGGA ACCCCTGAAT ACATCGCACC GGAGGTTATC
    CTTAGACAAG GTTACGGGAA GCCAGTGGAC TGGTGGGCGA TGGGCATAAT TCTGTATGAG
    TTCCTGGTTG GTTGCGTGCC ATTTTTTGGA GACACCCCAG AGGAGCTGTT TGGTCAGGTG
    ATCAGTGATG ACATTGTGTG GCCAGAAGCA GAGGAAGCTC TACCCGCCGA TGCCCAAAAT
    CTCATCTCCT GCCTCCTGCA GACAGATCCC TTACAAAGGC TTGGAACAGG GGGAGCCATT
    GAGGTGAAAA TTCACAGATT TTTCACTGAT TTGGACTGGA ATGGATTGCT CAGGCAGAAA
    GCTGAATTCA TCCCTCACTT GGAGGCAGAA GATGATACCA GTTACTTTGA CACTCGATCG
    GATCGGTACC AGCACATTAA TTCCTACGAT GAGGATGATA CCAATGATGA AGACTCGGTG
    GAGATCAGGC AGTTCTCATC CTGCACCCCA AGATTTAGTA AGGTGTATAG TAGTATGGAG
    CAGCTCTCCC AGCTGGAGCA GAAACCCCCC ACTCCTCCTA GAGACACCAA CAAACAAGAA
    AGGTCTGAGA AGACGACCAA GAGAGAGACG TTGGGGGGCG TCACCCTTCG AGACCGCTGC
    TGGAGGACCG TGTCTCCTGA AATGAAACAA TTCTCCGATG CGGCAGACGC AGAATCCAGC
    CCCCCCTTGG CTGCCCGGCG GCGCTTTTCT GCCTTAGTGG ACCCGACCCG CTATACGTCT
    CCCCTGGAGT ATGAGGTGGA GTACAGAGTG AGGGGAAGCA GCGGGCCGCT GCCCGCCCAA
    AGCAAGATCC CTCTGGGAGA GCTGGAGCCA GTAGACAAGT CTGTCGACTC TCAGCCCCAC
    CAGGAGGCGG ATTCCTCTCC TCGCGCCACA AACGAGATTG TCCTGCGCAG AGCCAGGCAC
    CAACACCTGT CCGGGGACAC CGAGAGGAGG AGAACCGGCG GGAAAGTCAT CAAGTCGGCA
    TCGACCACCG CCCTGTCTGT CATGATCCCT ACAGTTGAAC AGCACAGCAG CTCTCCCCTG
    GTCAGCCCCA TGTCCCCCCG CTCTCTGTCG TCCAATCCCT CCTCCCGGGA TTCCTCTCCG
    AGCCGCGACT ACACGCCGGC AGTGAGCAGC CTCCGATCCC CCATTTGCAT CCAACGCTCC
    GGGAAAAAAT ACGGTTTCAC CTTGCGGGCC ATCCGGGTGT ACATGGGGGA AAGTGATGTG
    TACAGCGTGC AGCACATTGT CTGGCACGTG GAGGAAGGAG GGCCTGCCCA TGAAGCGGGT
    CTCTGTGCCG GGGACCTCAT TACACACATT AACGGGGAGC CGGTGCACGG CCTGGTGCAC
    ACTGAAGTTG TGGAGCTGAT TTTAAAGAGC GGTAACAAGG TGTTAGTAAC CACCACCCCC
    TTTGAGAACA CCTCGATCCG AACCGGCCCA GCCAGGAGAA GCAGTTATCG ATCCAAAATG
    GCGCGGAGGA ACAAGCGGAT CGCTGGGAGA GAGGGGCAGG AGAGTAAGAA GCGGAGCTCT
    CTATTCCGTA AGATCACCAA GCAATCCAAT CTTCTGCACA CCAGCCGTTC CCTCTCCTCT
    CTGAGCCGCT CCCTCTCCTC AAGTGACAGT CTTCCAGGAT CACCCACTCA CAGCCTCTCG
    GCCCGTTCCC CGACCCATAG CTACCGCTCC ATGGACTCTG CTTACCTTGG TGCTTCTTCG
    CAGGGCAGTT CCCCCTCTTC CAGTACCCCT AATTCCCCTG CACCACCACC TCCTGCCTCA
    GCCTCTTCCT GTGCGTCAGC CTCCTCCTCC TCCACCACTC CTGCTCCCCC CTCCCCACAC
    ATCCGACCCA GCACCCTGCA TGGCTTATCT CCCAAACTGC ACCGCCAGTA TCGCTCAGCG
    CGATGTAAAT CTGCGGGTAA TATGCCCCTC TCTCCCCTTG CACACACCCC TTCCCCTACT
    GCAGCTGCCT CACCCCCTCT CCTACCAAGT CACAGCATTG GCAGCAGCCT GACCACTCAG
    AGCTTTCCCC CAAAACTGCA TCCATCTCCA CCAGTGTCAC GCCCTCGCCC AAAGAGCGCA
    GAGCCTCCAC GGTCCCCACT TCTTAAGAGA GTGCAGTCTG CAGAGAAACT GGGTGGGGGA
    GGAGGTGGTG GGTCACCCAG AAAACAGGAG CCGGGAGGGG GAGGAACAAT AGAGGGAGAT
    TTAGCAGAAG CCATTGGTGT GAGGAGCCAG CCAGTAAGGA GAGTGGGTAG GCAGGAGTCT
    CCTCGCAGTC TTTGTGGAGG GGAAAATATG ATAGGAGAGA ACTTTGAGAG GCTGGAAGGA
    GAAGGAGCGA TAGGACTATC GCCGCTAGGA GACAGTCTGA AATGTAGTGA GGGTAAAGTC
    CAAGCAGCCG TTAAGGTTGG AGTAGGATCA AGGACTACTC CCCCATTTTC TCCCCAAGAA
    CCTCCAGCCG CATCAACTGC AGTCCTTCCC CCCCAAAGTG ACCCTTCCCT TGCTATGGTA
    GGAAGGTGCC AAGTGAAAAC CCTAAGTCCT GTACAAGAAC ATGAAGGTAG AAGGGGGGAA
    GGAGAGGACG GGGGAGCAGA GGGAGAGGGA AGAAAGGGAG ATTATGAGGA GGAACAAGGA
    AGAACAAGAG AACAATGGTA CTTAGAGGTG GTAGAAGAGT TGACTACAGT AGGGAAAAGC
    AGCATGCATG CTGGGAGTAA AGCGACTCCC TCTAACCTCA CAGCCGACTC TAGTATCTAC
    CATTCCCACT CAAATAAGAG TAAAGTCGCT GTGGTTGACT CTATGAAGAG CACACCCTCT
    CACCCTACAG GTACAACTCT GGAGCAGTCT CTTTCTTTGA AAGGGATTAG ATCTGATGCC
    GACTCTAAAA AGGGGCAAGA TCCTAAAAAC TGCCAGATTA CTCCTCTTCT TCCCGTAAGG
    GAGGGCCTAA ATGTTGAACC AATTCAAAAT GGTTCTACTC ACCCTCCTGA ACTTTCCCAC
    GTGGCCAAGG AGCAAAGTAT CGACCATCTA AAAGGTCCAT CATCTTCTGG CCAACAAATG
    AAAAGCACTA GCACTTTCCT ACCTACTCAG CTTCACCCAA CCACTGGTAG AAAGCTGGAG
    GCTGATAATG TAAGTAGGCA TTTACTCTGT ACAGAGGGTA CAAAGTATGG TCTGATTAAA
    CCAACAGATT CTCTGTCTAT TAGTATTAAA GGGGAGAAGG CCAGTTTAGC AAAAAGCCCC
    CAGGATGATC CAGTAAAGGC CGCTGATCAT ACTACTGGGG TGCAAGGACC TGCTCCTGCA
    AAAGGACCAG CAATTTCACC TGTAAATATT AAAATCGGCA GAGAAGGATC TGAACAGACA
    AGGGTGACCA CTACTGACCC AACTATTAAT AGGCTGAGCG AAACACAAAC TAATCAGAGA
    CCTGCAGCTG CCACTGGTCT TGCTAGCAAT ATGGTACTTA GGGAGATGGA GAAGAAGGAA
    GAGTTCAGTT CAGCCCAGAA TATAAAGAAA CCAAGTGGAG CAGAATTAGA ACAGAAGCGG
    GTGGCCATTG TAGTCCCGAT TGTAACTAGA CCCAGTGATG TAAAGGCTGA CACAACAAGG
    GTAGTATCTG CTCCCCTGAC TGTTAATAGA CCTGCCGCAG GAGAGAGTGA CCAAGCAAAA
    GTGACCACTG TTACCCCAAC TATTGATAAA GTCAGTGGCC CAGAAAGTGC CTCTGCCAAG
    GTGACCGCCG TTGCCTCAAT GAGTAGCGCT AACAAAGCTG TTAATGAACC ACCAAGCCTG
    TTCCCCGATA ATAAAGTAGC CGACTCCAAT CAAATAAGGT TCTCGTCACA GAATAACAAG
    GGCGCGAGAG AATCAGAAAG TGACCAGATA AGGGCAACGG GCAATATTCC CGTTATTAAC
    CAGACCGCTC AAACAACGGT TCCTTCCCCA ACACCCACAA ATGGCCAAGG ACCCCCTATA
    ATTATCCGAG TTAGTGGGGT TCCATGTGAT GCCCAGGGCG GGCTGTGTCC TGAGAAACCT
    TCCAGCCCCC AAAGAGCGCC CACCACTACC AGCCAACCCC CTTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755303.1
    CDS64..5751
    Translation

    Target ORF information:

    RefSeq Version XM_031899443.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis microtubule associated serine/threonine kinase 1 (mast1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899443.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGATGATT CTGGCATTAT CAGACGCAGG AGGCTGCAGA AGGATCTGCC CCTGCCGAGG 
    AAGAGCAGCG GTTGCAGAAG CAGCAACAGA AAGAGTTTAA TCCTCACAAG CAGCAGTTCC
    CCCACTCTCC CCCGGCCCCA CTCCCCCCTG CCGGGGCATA TAGGTGGGAG CCCTATGGAC
    AGTCCCAGGA ACTTCTCCCC CAGCGCCGCT GCTCACTTCT CCTTTGCATC ATCTCGCAGC
    AGAACCGATG GCCGTCGCTG GTCCTTGGCC TCCCTCCCGT CGTCCGGCTA TGGGACAAAT
    ACCCCGAGTT CCACGGTGTC GTCCTCCTCC TCCTCTCAGG AACGCCTACA CCAGCTCCCC
    TTCCAGCCCA CACTGGATGA GCTCCACTTC CTTTCCAAAC ACTTTGGCAG CACAGAAAGT
    ATCCCCGATG ATGATGGGGG GCGCAGGTCA CCTGCGGTCA GACCACGATC CCGCAGCTTA
    AGTCCTGGCC GATCTCCCTC CTCTTATGAC AATGAAATTG TGATGATGAA CCATGTTTAT
    AAAGAGAGGT TTCCCAAGGC CACAGCGCAG ATGGAGGATA GGCTACGGAT GTTCATCTCC
    TCCTTCTCCC CCGAGAATGT CCTACCACTG GCGGACGGAC TCCTCAACTT TATCCACCAT
    CAGATCGTGG AGCTGGCGAG GGACTGCCTG AACAGGAGCA CCGAGGGCAT GATCACAACC
    GCCCACTTTT ACCAACTACA GGAGAATCTG GAGAAGCTGC TGAATGATGC AGTGGAACGT
    TCTTCAGAGG GATCAGAAGT TGCCTTCATT GCTCAACTGA TGAAAAATCT TTTAATCATC
    ATCTCTCGAC CTGCTCGTCT GCTGGAGTGT CTGGAATTTA ACCCTGAAGA GTTCTATCAC
    ATGCTGGAGG TAGCCGAGGA CCAGGTGAAA GAAGGAAACT TGAAAAAAGA CATACCCCAA
    TACATCCTGA GACAGTTGGG ATTAACCAGG GATTCCTTCC CAGAGGTAGC CGCTCCCGAG
    GAACCAGTGA CCCTGGAAGC ACAAACCTCA GAAGCAGAGG ACAACGTAGA GGCAAAGAGT
    GGGGCGGCCC ATAAACCCAT CAGACCACCC GAGGAAAGTG ACTTTGAAAA TATAAAGCTC
    ATCAGTAACG GAGCCTATGG AGCGGTGTAT TTGGTGCGTC ACCGGGAGAC GCGGCAACGC
    TTTGCCATGA AGAAGATCAA CAAGCAGAAC TTGCGTCTGA GGAAACAAGT CCACCAGGCC
    TTTGTAGAAC GGGACATTCT GACCTTTGCT CAGAACCCCT TTGTGGTCAG CATGTTCTGC
    TCCTTCCAGA CCCGTAGGCA CCTCTGTATG GTGATGGAGT ATGTGGAAGG GGGTGACTGT
    GCCACCCTAC TGAAGCACAT TGGTGCTCTC CCATTGGAAA TGGCGCGGTT ATATTTCGCA
    GAGACGGTTC TAGCTCTGGA ATACCTACAT AATTATGGGA TTGTGCACCG AGATCTGAAA
    CCGGACAATC TCCTAATCAC TTCGATGGGT CACATAAAGC TTACAGATTT CGGCCTGTCC
    AAGATGGGGC TTATGAACCT CACCACTAAT CTCTATGAAG GACACATCGA GAGCGAAGCA
    AGGGAATTCC TGGACAAGCA GGTCTGTGGA ACCCCTGAAT ACATCGCACC GGAGGTTATC
    CTTAGACAAG GTTACGGGAA GCCAGTGGAC TGGTGGGCGA TGGGCATAAT TCTGTATGAG
    TTCCTGGTTG GTTGCGTGCC ATTTTTTGGA GACACCCCAG AGGAGCTGTT TGGTCAGGTG
    ATCAGTGATG ACATTGTGTG GCCAGAAGCA GAGGAAGCTC TACCCGCCGA TGCCCAAAAT
    CTCATCTCCT GCCTCCTGCA GACAGATCCC TTACAAAGGC TTGGAACAGG GGGAGCCATT
    GAGGTGAAAA TTCACAGATT TTTCACTGAT TTGGACTGGA ATGGATTGCT CAGGCAGAAA
    GCTGAATTCA TCCCTCACTT GGAGGCAGAA GATGATACCA GTTACTTTGA CACTCGATCG
    GATCGGTACC AGCACATTAA TTCCTACGAT GAGGATGATA CCAATGATGA AGACTCGGTG
    GAGATCAGGC AGTTCTCATC CTGCACCCCA AGATTTAGTA AGGTGTATAG TAGTATGGAG
    CAGCTCTCCC AGCTGGAGCA GAAACCCCCC ACTCCTCCTA GAGACACCAA CAAACAAGAA
    AGGTCTGAGA AGACGACCAA GAGAGAGACG TTGGGGGGCG TCACCCTTCG AGACCGCTGC
    TGGAGGACCG TGTCTCCTGA AATGAAACAA TTCTCCGATG CGGCAGACGC AGAATCCAGC
    CCCCCCTTGG CTGCCCGGCG GCGCTTTTCT GCCTTAGTGG ACCCGACCCG CTATACGTCT
    CCCCTGGAGT ATGAGGTGGA GTACAGAGTG AGGGGAAGCA GCGGGCCGCT GCCCGCCCAA
    AGCAAGATCC CTCTGGGAGA GCTGGAGCCA GTAGACAAGT CTGTCGACTC TCAGCCCCAC
    CAGGAGGCGG ATTCCTCTCC TCGCGCCACA AACGAGATTG TCCTGCGCAG AGCCAGGCAC
    CAACACCTGT CCGGGGACAC CGAGAGGAGG AGAACCGGCG GGAAAGTCAT CAAGTCGGCA
    TCGACCACCG CCCTGTCTGT CATGATCCCT ACAGTTGAAC AGCACAGCAG CTCTCCCCTG
    GTCAGCCCCA TGTCCCCCCG CTCTCTGTCG TCCAATCCCT CCTCCCGGGA TTCCTCTCCG
    AGCCGCGACT ACACGCCGGC AGTGAGCAGC CTCCGATCCC CCATTTGCAT CCAACGCTCC
    GGGAAAAAAT ACGGTTTCAC CTTGCGGGCC ATCCGGGTGT ACATGGGGGA AAGTGATGTG
    TACAGCGTGC AGCACATTGT CTGGCACGTG GAGGAAGGAG GGCCTGCCCA TGAAGCGGGT
    CTCTGTGCCG GGGACCTCAT TACACACATT AACGGGGAGC CGGTGCACGG CCTGGTGCAC
    ACTGAAGTTG TGGAGCTGAT TTTAAAGAGC GGTAACAAGG TGTTAGTAAC CACCACCCCC
    TTTGAGAACA CCTCGATCCG AACCGGCCCA GCCAGGAGAA GCAGTTATCG ATCCAAAATG
    GCGCGGAGGA ACAAGCGGAT CGCTGGGAGA GAGGGGCAGG AGAGTAAGAA GCGGAGCTCT
    CTATTCCGTA AGATCACCAA GCAATCCAAT CTTCTGCACA CCAGCCGTTC CCTCTCCTCT
    CTGAGCCGCT CCCTCTCCTC AAGTGACAGT CTTCCAGGAT CACCCACTCA CAGCCTCTCG
    GCCCGTTCCC CGACCCATAG CTACCGCTCC ATGGACTCTG CTTACCTTGG TGCTTCTTCG
    CAGGGCAGTT CCCCCTCTTC CAGTACCCCT AATTCCCCTG CACCACCACC TCCTGCCTCA
    GCCTCTTCCT GTGCGTCAGC CTCCTCCTCC TCCACCACTC CTGCTCCCCC CTCCCCACAC
    ATCCGACCCA GCACCCTGCA TGGCTTATCT CCCAAACTGC ACCGCCAGTA TCGCTCAGCG
    CGATGTAAAT CTGCGGGTAA TATGCCCCTC TCTCCCCTTG CACACACCCC TTCCCCTACT
    GCAGCTGCCT CACCCCCTCT CCTACCAAGT CACAGCATTG GCAGCAGCCT GACCACTCAG
    AGCTTTCCCC CAAAACTGCA TCCATCTCCA CCAGTGTCAC GCCCTCGCCC AAAGAGCGCA
    GAGCCTCCAC GGTCCCCACT TCTTAAGAGA GTGCAGTCTG CAGAGAAACT GGGTGGGGGA
    GGAGGTGGTG GGTCACCCAG AAAACAGGAG CCGGGAGGGG GAGGAACAAT AGAGGGAGAT
    TTAGCAGAAG CCATTGGTGT GAGGAGCCAG CCAGTAAGGA GAGTGGGTAG GCAGGAGTCT
    CCTCGCAGTC TTTGTGGAGG GGAAAATATG ATAGGAGAGA ACTTTGAGAG GCTGGAAGGA
    GAAGGAGCGA TAGGACTATC GCCGCTAGGA GACAGTCTGA AATGTAGTGA GGGTAAAGTC
    CAAGCAGCCG TTAAGGTTGG AGTAGGATCA AGGACTACTC CCCCATTTTC TCCCCAAGAA
    CCTCCAGCCG CATCAACTGC AGTCCTTCCC CCCCAAAGTG ACCCTTCCCT TGCTATGGTA
    GGAAGGTGCC AAGTGAAAAC CCTAAGTCCT GTACAAGAAC ATGAAGGTAG AAGGGGGGAA
    GGAGAGGACG GGGGAGCAGA GGGAGAGGGA AGAAAGGGAG ATTATGAGGA GGAACAAGGA
    AGAACAAGAG AACAATGGTA CTTAGAGGTG GTAGAAGAGT TGACTACAGT AGGGAAAAGC
    AGCATGCATG CTGGGAGTAA AGCGACTCCC TCTAACCTCA CAGCCGACTC TAGTATCTAC
    CATTCCCACT CAAATAAGAG TAAAGTCGCT GTGGTTGACT CTATGAAGAG CACACCCTCT
    CACCCTACAG GTACAACTCT GGAGCAGTCT CTTTCTTTGA AAGGGATTAG ATCTGATGCC
    GACTCTAAAA AGGGGCAAGA TCCTAAAAAC TGCCAGATTA CTCCTCTTCT TCCCGTAAGG
    GAGGGCCTAA ATGTTGAACC AATTCAAAAT GGTTCTACTC ACCCTCCTGA ACTTTCCCAC
    GTGGCCAAGG AGCAAAGTAT CGACCATCTA AAAGGTCCAT CATCTTCTGG CCAACAAATG
    AAAAGCACTA GCACTTTCCT ACCTACTCAG CTTCACCCAA CCACTGGTAG AAAGCTGGAG
    GCTGATAATG TAAGTAGGCA TTTACTCTGT ACAGAGGGTA CAAAGTATGG TCTGATTAAA
    CCAACAGATT CTCTGTCTAT TAGTATTAAA GGGGAGAAGG CCAGTTTAGC AAAAAGCCCC
    CAGGATGATC CAGTAAAGGC CGCTGATCAT ACTACTGGGG TGCAAGGACC TGCTCCTGCA
    AAAGGACCAG CAATTTCACC TGTAAATATT AAAATCGGCA GAGAAGGATC TGAACAGACA
    AGGGTGACCA CTACTGACCC AACTATTAAT AGGCTGAGCG AAACACAAAC TAATCAGAGA
    CCTGCAGCTG CCACTGGTCT TGCTAGCAAT ATGGTACTTA GGGAGATGGA GAAGAAGGAA
    GAGTTCAGTT CAGCCCAGAA TATAAAGAAA CCAAGTGGAG CAGAATTAGA ACAGAAGCGG
    GTGGCCATTG TAGTCCCGAT TGTAACTAGA CCCAGTGATG TAAAGGCTGA CACAACAAGG
    GTAGTATCTG CTCCCCTGAC TGTTAATAGA CCTGCCGCAG GAGAGAGTGA CCAAGCAAAA
    GTGACCACTG TTACCCCAAC TATTGATAAA GTCAGTGGCC CAGAAAGTGC CTCTGCCAAG
    GTGACCGCCG TTGCCTCAAT GAGTAGCGCT AACAAAGCTG TTAATGAACC ACCAAGCCTG
    TTCCCCGATA ATAAAGTAGC CGACTCCAAT CAAATAAGGT TCTCGTCACA GAATAACAAG
    GGCGCGAGAG AATCAGAAAG TGACCAGATA AGGGCAACGG GCAATATTCC CGTTATTAAC
    CAGACCGCTC AAACAACGGT TCCTTCCCCA ACACCCACAA ATGGCCAAGG ACCCCCTATA
    ATTATCCGAG TTAGTGGGGT TCCATGTGAT GCCCAGGGCG GGCTGTGTCC TGAGAAACCT
    TCCAGCCCCC AAAGAGCGCC CACCACTACC AGCCAACCCC CTTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55395
    Clone ID Related Accession (Same CDS sequence) XM_031899444.1
    Accession Version XM_031899444.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5685bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product microtubule-associated serine/threonine-protein kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGATGATT CTGGCATTAT CAGACGCAGG AGGCTGCAGA AGGATCTGCC CCTGCCGAGG 
    AAGAGCAGCG GTTGCAGAAG CAGCAACAGA AAGAGTTTAA TCCTCACAAG CAGCAGTTCC
    CCCACTCTCC CCCGGCCCCA CTCCCCCCTG CCGGGGCATA TAGGTGGGAG CCCTATGGAC
    AGTCCCAGGA ACTTCTCCCC CAGCGCCGCT GCTCACTTCT CCTTTGCATC ATCTCGCAGA
    ACCGATGGCC GTCGCTGGTC CTTGGCCTCC CTCCCGTCGT CCGGCTATGG GACAAATACC
    CCGAGTTCCA CGGTGTCGTC CTCCTCCTCC TCTCAGGAAC GCCTACACCA GCTCCCCTTC
    CAGCCCACAC TGGATGAGCT CCACTTCCTT TCCAAACACT TTGGCAGCAC AGAAAGTATC
    CCCGATGATG ATGGGGGGCG CAGGTCACCT GCGGTCAGAC CACGATCCCG CAGCTTAAGT
    CCTGGCCGAT CTCCCTCCTC TTATGACAAT GAAATTGTGA TGATGAACCA TGTTTATAAA
    GAGAGGTTTC CCAAGGCCAC AGCGCAGATG GAGGATAGGC TACGGATGTT CATCTCCTCC
    TTCTCCCCCG AGAATGTCCT ACCACTGGCG GACGGACTCC TCAACTTTAT CCACCATCAG
    ATCGTGGAGC TGGCGAGGGA CTGCCTGAAC AGGAGCACCG AGGGCATGAT CACAACCGCC
    CACTTTTACC AACTACAGGA GAATCTGGAG AAGCTGCTGA ATGATGCAGT GGAACGTTCT
    TCAGAGGGAT CAGAAGTTGC CTTCATTGCT CAACTGATGA AAAATCTTTT AATCATCATC
    TCTCGACCTG CTCGTCTGCT GGAGTGTCTG GAATTTAACC CTGAAGAGTT CTATCACATG
    CTGGAGGTAG CCGAGGACCA GGTGAAAGAA GGAAACTTGA AAAAAGACAT ACCCCAATAC
    ATCCTGAGAC AGTTGGGATT AACCAGGGAT TCCTTCCCAG AGGTAGCCGC TCCCGAGGAA
    CCAGTGACCC TGGAAGCACA AACCTCAGAA GCAGAGGACA ACGTAGAGGC AAAGAGTGGG
    GCGGCCCATA AACCCATCAG ACCACCCGAG GAAAGTGACT TTGAAAATAT AAAGCTCATC
    AGTAACGGAG CCTATGGAGC GGTGTATTTG GTGCGTCACC GGGAGACGCG GCAACGCTTT
    GCCATGAAGA AGATCAACAA GCAGAACTTG CGTCTGAGGA AACAAGTCCA CCAGGCCTTT
    GTAGAACGGG ACATTCTGAC CTTTGCTCAG AACCCCTTTG TGGTCAGCAT GTTCTGCTCC
    TTCCAGACCC GTAGGCACCT CTGTATGGTG ATGGAGTATG TGGAAGGGGG TGACTGTGCC
    ACCCTACTGA AGCACATTGG TGCTCTCCCA TTGGAAATGG CGCGGTTATA TTTCGCAGAG
    ACGGTTCTAG CTCTGGAATA CCTACATAAT TATGGGATTG TGCACCGAGA TCTGAAACCG
    GACAATCTCC TAATCACTTC GATGGGTCAC ATAAAGCTTA CAGATTTCGG CCTGTCCAAG
    ATGGGGCTTA TGAACCTCAC CACTAATCTC TATGAAGGAC ACATCGAGAG CGAAGCAAGG
    GAATTCCTGG ACAAGCAGGT CTGTGGAACC CCTGAATACA TCGCACCGGA GGTTATCCTT
    AGACAAGGTT ACGGGAAGCC AGTGGACTGG TGGGCGATGG GCATAATTCT GTATGAGTTC
    CTGGTTGGTT GCGTGCCATT TTTTGGAGAC ACCCCAGAGG AGCTGTTTGG TCAGGTGATC
    AGTGATGACA TTGTGTGGCC AGAAGCAGAG GAAGCTCTAC CCGCCGATGC CCAAAATCTC
    ATCTCCTGCC TCCTGCAGAC AGATCCCTTA CAAAGGCTTG GAACAGGGGG AGCCATTGAG
    GTGAAAATTC ACAGATTTTT CACTGATTTG GACTGGAATG GATTGCTCAG GCAGAAAGCT
    GAATTCATCC CTCACTTGGA GGCAGAAGAT GATACCAGTT ACTTTGACAC TCGATCGGAT
    CGGTACCAGC ACATTAATTC CTACGATGAG GATGATACCA ATGATGAAGA CTCGGTGGAG
    ATCAGGCAGT TCTCATCCTG CACCCCAAGA TTTAGTAAGG TGTATAGTAG TATGGAGCAG
    CTCTCCCAGC TGGAGCAGAA ACCCCCCACT CCTCCTAGAG ACACCAACAA ACAAGAAAGG
    TCTGAGAAGA CGACCAAGAG AGAGACGTTG GGGGGCGTCA CCCTTCGAGA CCGCTGCTGG
    AGGACCGTGT CTCCTGAAAT GAAACAATTC TCCGATGCGG CAGACGCAGA ATCCAGCCCC
    CCCTTGGCTG CCCGGCGGCG CTTTTCTGCC TTAGTGGACC CGACCCGCTA TACGTCTCCC
    CTGGAGTATG AGGTGGAGTA CAGAGTGAGG GGAAGCAGCG GGCCGCTGCC CGCCCAAAGC
    AAGATCCCTC TGGGAGAGCT GGAGCCAGTA GACAAGTCTG TCGACTCTCA GCCCCACCAG
    GAGGCGGATT CCTCTCCTCG CGCCACAAAC GAGATTGTCC TGCGCAGAGC CAGGCACCAA
    CACCTGTCCG GGGACACCGA GAGGAGGAGA ACCGGCGGGA AAGTCATCAA GTCGGCATCG
    ACCACCGCCC TGTCTGTCAT GATCCCTACA GTTGAACAGC ACAGCAGCTC TCCCCTGGTC
    AGCCCCATGT CCCCCCGCTC TCTGTCGTCC AATCCCTCCT CCCGGGATTC CTCTCCGAGC
    CGCGACTACA CGCCGGCAGT GAGCAGCCTC CGATCCCCCA TTTGCATCCA ACGCTCCGGG
    AAAAAATACG GTTTCACCTT GCGGGCCATC CGGGTGTACA TGGGGGAAAG TGATGTGTAC
    AGCGTGCAGC ACATTGTCTG GCACGTGGAG GAAGGAGGGC CTGCCCATGA AGCGGGTCTC
    TGTGCCGGGG ACCTCATTAC ACACATTAAC GGGGAGCCGG TGCACGGCCT GGTGCACACT
    GAAGTTGTGG AGCTGATTTT AAAGAGCGGT AACAAGGTGT TAGTAACCAC CACCCCCTTT
    GAGAACACCT CGATCCGAAC CGGCCCAGCC AGGAGAAGCA GTTATCGATC CAAAATGGCG
    CGGAGGAACA AGCGGATCGC TGGGAGAGAG GGGCAGGAGA GTAAGAAGCG GAGCTCTCTA
    TTCCGTAAGA TCACCAAGCA ATCCAATCTT CTGCACACCA GCCGTTCCCT CTCCTCTCTG
    AGCCGCTCCC TCTCCTCAAG TGACAGTCTT CCAGGATCAC CCACTCACAG CCTCTCGGCC
    CGTTCCCCGA CCCATAGCTA CCGCTCCATG GACTCTGCTT ACCTTGGTGC TTCTTCGCAG
    GGCAGTTCCC CCTCTTCCAG TACCCCTAAT TCCCCTGCAC CACCACCTCC TGCCTCAGCC
    TCTTCCTGTG CGTCAGCCTC CTCCTCCTCC ACCACTCCTG CTCCCCCCTC CCCACACATC
    CGACCCAGCA CCCTGCATGG CTTATCTCCC AAACTGCACC GCCAGTATCG CTCAGCGCGA
    TGTAAATCTG CGGGTAATAT GCCCCTCTCT CCCCTTGCAC ACACCCCTTC CCCTACTGCA
    GCTGCCTCAC CCCCTCTCCT ACCAAGTCAC AGCATTGGCA GCAGCCTGAC CACTCAGAGC
    TTTCCCCCAA AACTGCATCC ATCTCCACCA GTGTCACGCC CTCGCCCAAA GAGCGCAGAG
    CCTCCACGGT CCCCACTTCT TAAGAGAGTG CAGTCTGCAG AGAAACTGGG TGGGGGAGGA
    GGTGGTGGGT CACCCAGAAA ACAGGAGCCG GGAGGGGGAG GAACAATAGA GGGAGATTTA
    GCAGAAGCCA TTGGTGTGAG GAGCCAGCCA GTAAGGAGAG TGGGTAGGCA GGAGTCTCCT
    CGCAGTCTTT GTGGAGGGGA AAATATGATA GGAGAGAACT TTGAGAGGCT GGAAGGAGAA
    GGAGCGATAG GACTATCGCC GCTAGGAGAC AGTCTGAAAT GTAGTGAGGG TAAAGTCCAA
    GCAGCCGTTA AGGTTGGAGT AGGATCAAGG ACTACTCCCC CATTTTCTCC CCAAGAACCT
    CCAGCCGCAT CAACTGCAGT CCTTCCCCCC CAAAGTGACC CTTCCCTTGC TATGGTAGGA
    AGGTGCCAAG TGAAAACCCT AAGTCCTGTA CAAGAACATG AAGGTAGAAG GGGGGAAGGA
    GAGGACGGGG GAGCAGAGGG AGAGGGAAGA AAGGGAGATT ATGAGGAGGA ACAAGGAAGA
    ACAAGAGAAC AATGGTACTT AGAGGTGGTA GAAGAGTTGA CTACAGTAGG GAAAAGCAGC
    ATGCATGCTG GGAGTAAAGC GACTCCCTCT AACCTCACAG CCGACTCTAG TATCTACCAT
    TCCCACTCAA ATAAGAGTAA AGTCGCTGTG GTTGACTCTA TGAAGAGCAC ACCCTCTCAC
    CCTACAGGTA CAACTCTGGA GCAGTCTCTT TCTTTGAAAG GGATTAGATC TGATGCCGAC
    TCTAAAAAGG GGCAAGATCC TAAAAACTGC CAGATTACTC CTCTTCTTCC CGTAAGGGAG
    GGCCTAAATG TTGAACCAAT TCAAAATGGT TCTACTCACC CTCCTGAACT TTCCCACGTG
    GCCAAGGAGC AAAGTATCGA CCATCTAAAA GGTCCATCAT CTTCTGGCCA ACAAATGAAA
    AGCACTAGCA CTTTCCTACC TACTCAGCTT CACCCAACCA CTGGTAGAAA GCTGGAGGCT
    GATAATGTAA GTAGGCATTT ACTCTGTACA GAGGGTACAA AGTATGGTCT GATTAAACCA
    ACAGATTCTC TGTCTATTAG TATTAAAGGG GAGAAGGCCA GTTTAGCAAA AAGCCCCCAG
    GATGATCCAG TAAAGGCCGC TGATCATACT ACTGGGGTGC AAGGACCTGC TCCTGCAAAA
    GGACCAGCAA TTTCACCTGT AAATATTAAA ATCGGCAGAG AAGGATCTGA ACAGACAAGG
    GTGACCACTA CTGACCCAAC TATTAATAGG CTGAGCGAAA CACAAACTAA TCAGAGACCT
    GCAGCTGCCA CTGGTCTTGC TAGCAATATG GTACTTAGGG AGATGGAGAA GAAGGAAGAG
    TTCAGTTCAG CCCAGAATAT AAAGAAACCA AGTGGAGCAG AATTAGAACA GAAGCGGGTG
    GCCATTGTAG TCCCGATTGT AACTAGACCC AGTGATGTAA AGGCTGACAC AACAAGGGTA
    GTATCTGCTC CCCTGACTGT TAATAGACCT GCCGCAGGAG AGAGTGACCA AGCAAAAGTG
    ACCACTGTTA CCCCAACTAT TGATAAAGTC AGTGGCCCAG AAAGTGCCTC TGCCAAGGTG
    ACCGCCGTTG CCTCAATGAG TAGCGCTAAC AAAGCTGTTA ATGAACCACC AAGCCTGTTC
    CCCGATAATA AAGTAGCCGA CTCCAATCAA ATAAGGTTCT CGTCACAGAA TAACAAGGGC
    GCGAGAGAAT CAGAAAGTGA CCAGATAAGG GCAACGGGCA ATATTCCCGT TATTAACCAG
    ACCGCTCAAA CAACGGTTCC TTCCCCAACA CCCACAAATG GCCAAGGACC CCCTATAATT
    ATCCGAGTTA GTGGGGTTCC ATGTGATGCC CAGGGCGGGC TGTGTCCTGA GAAACCTTCC
    AGCCCCCAAA GAGCGCCCAC CACTACCAGC CAACCCCCTT CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755304.1
    CDS64..5748
    Translation

    Target ORF information:

    RefSeq Version XM_031899444.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis microtubule associated serine/threonine kinase 1 (mast1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899444.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGATGATT CTGGCATTAT CAGACGCAGG AGGCTGCAGA AGGATCTGCC CCTGCCGAGG 
    AAGAGCAGCG GTTGCAGAAG CAGCAACAGA AAGAGTTTAA TCCTCACAAG CAGCAGTTCC
    CCCACTCTCC CCCGGCCCCA CTCCCCCCTG CCGGGGCATA TAGGTGGGAG CCCTATGGAC
    AGTCCCAGGA ACTTCTCCCC CAGCGCCGCT GCTCACTTCT CCTTTGCATC ATCTCGCAGA
    ACCGATGGCC GTCGCTGGTC CTTGGCCTCC CTCCCGTCGT CCGGCTATGG GACAAATACC
    CCGAGTTCCA CGGTGTCGTC CTCCTCCTCC TCTCAGGAAC GCCTACACCA GCTCCCCTTC
    CAGCCCACAC TGGATGAGCT CCACTTCCTT TCCAAACACT TTGGCAGCAC AGAAAGTATC
    CCCGATGATG ATGGGGGGCG CAGGTCACCT GCGGTCAGAC CACGATCCCG CAGCTTAAGT
    CCTGGCCGAT CTCCCTCCTC TTATGACAAT GAAATTGTGA TGATGAACCA TGTTTATAAA
    GAGAGGTTTC CCAAGGCCAC AGCGCAGATG GAGGATAGGC TACGGATGTT CATCTCCTCC
    TTCTCCCCCG AGAATGTCCT ACCACTGGCG GACGGACTCC TCAACTTTAT CCACCATCAG
    ATCGTGGAGC TGGCGAGGGA CTGCCTGAAC AGGAGCACCG AGGGCATGAT CACAACCGCC
    CACTTTTACC AACTACAGGA GAATCTGGAG AAGCTGCTGA ATGATGCAGT GGAACGTTCT
    TCAGAGGGAT CAGAAGTTGC CTTCATTGCT CAACTGATGA AAAATCTTTT AATCATCATC
    TCTCGACCTG CTCGTCTGCT GGAGTGTCTG GAATTTAACC CTGAAGAGTT CTATCACATG
    CTGGAGGTAG CCGAGGACCA GGTGAAAGAA GGAAACTTGA AAAAAGACAT ACCCCAATAC
    ATCCTGAGAC AGTTGGGATT AACCAGGGAT TCCTTCCCAG AGGTAGCCGC TCCCGAGGAA
    CCAGTGACCC TGGAAGCACA AACCTCAGAA GCAGAGGACA ACGTAGAGGC AAAGAGTGGG
    GCGGCCCATA AACCCATCAG ACCACCCGAG GAAAGTGACT TTGAAAATAT AAAGCTCATC
    AGTAACGGAG CCTATGGAGC GGTGTATTTG GTGCGTCACC GGGAGACGCG GCAACGCTTT
    GCCATGAAGA AGATCAACAA GCAGAACTTG CGTCTGAGGA AACAAGTCCA CCAGGCCTTT
    GTAGAACGGG ACATTCTGAC CTTTGCTCAG AACCCCTTTG TGGTCAGCAT GTTCTGCTCC
    TTCCAGACCC GTAGGCACCT CTGTATGGTG ATGGAGTATG TGGAAGGGGG TGACTGTGCC
    ACCCTACTGA AGCACATTGG TGCTCTCCCA TTGGAAATGG CGCGGTTATA TTTCGCAGAG
    ACGGTTCTAG CTCTGGAATA CCTACATAAT TATGGGATTG TGCACCGAGA TCTGAAACCG
    GACAATCTCC TAATCACTTC GATGGGTCAC ATAAAGCTTA CAGATTTCGG CCTGTCCAAG
    ATGGGGCTTA TGAACCTCAC CACTAATCTC TATGAAGGAC ACATCGAGAG CGAAGCAAGG
    GAATTCCTGG ACAAGCAGGT CTGTGGAACC CCTGAATACA TCGCACCGGA GGTTATCCTT
    AGACAAGGTT ACGGGAAGCC AGTGGACTGG TGGGCGATGG GCATAATTCT GTATGAGTTC
    CTGGTTGGTT GCGTGCCATT TTTTGGAGAC ACCCCAGAGG AGCTGTTTGG TCAGGTGATC
    AGTGATGACA TTGTGTGGCC AGAAGCAGAG GAAGCTCTAC CCGCCGATGC CCAAAATCTC
    ATCTCCTGCC TCCTGCAGAC AGATCCCTTA CAAAGGCTTG GAACAGGGGG AGCCATTGAG
    GTGAAAATTC ACAGATTTTT CACTGATTTG GACTGGAATG GATTGCTCAG GCAGAAAGCT
    GAATTCATCC CTCACTTGGA GGCAGAAGAT GATACCAGTT ACTTTGACAC TCGATCGGAT
    CGGTACCAGC ACATTAATTC CTACGATGAG GATGATACCA ATGATGAAGA CTCGGTGGAG
    ATCAGGCAGT TCTCATCCTG CACCCCAAGA TTTAGTAAGG TGTATAGTAG TATGGAGCAG
    CTCTCCCAGC TGGAGCAGAA ACCCCCCACT CCTCCTAGAG ACACCAACAA ACAAGAAAGG
    TCTGAGAAGA CGACCAAGAG AGAGACGTTG GGGGGCGTCA CCCTTCGAGA CCGCTGCTGG
    AGGACCGTGT CTCCTGAAAT GAAACAATTC TCCGATGCGG CAGACGCAGA ATCCAGCCCC
    CCCTTGGCTG CCCGGCGGCG CTTTTCTGCC TTAGTGGACC CGACCCGCTA TACGTCTCCC
    CTGGAGTATG AGGTGGAGTA CAGAGTGAGG GGAAGCAGCG GGCCGCTGCC CGCCCAAAGC
    AAGATCCCTC TGGGAGAGCT GGAGCCAGTA GACAAGTCTG TCGACTCTCA GCCCCACCAG
    GAGGCGGATT CCTCTCCTCG CGCCACAAAC GAGATTGTCC TGCGCAGAGC CAGGCACCAA
    CACCTGTCCG GGGACACCGA GAGGAGGAGA ACCGGCGGGA AAGTCATCAA GTCGGCATCG
    ACCACCGCCC TGTCTGTCAT GATCCCTACA GTTGAACAGC ACAGCAGCTC TCCCCTGGTC
    AGCCCCATGT CCCCCCGCTC TCTGTCGTCC AATCCCTCCT CCCGGGATTC CTCTCCGAGC
    CGCGACTACA CGCCGGCAGT GAGCAGCCTC CGATCCCCCA TTTGCATCCA ACGCTCCGGG
    AAAAAATACG GTTTCACCTT GCGGGCCATC CGGGTGTACA TGGGGGAAAG TGATGTGTAC
    AGCGTGCAGC ACATTGTCTG GCACGTGGAG GAAGGAGGGC CTGCCCATGA AGCGGGTCTC
    TGTGCCGGGG ACCTCATTAC ACACATTAAC GGGGAGCCGG TGCACGGCCT GGTGCACACT
    GAAGTTGTGG AGCTGATTTT AAAGAGCGGT AACAAGGTGT TAGTAACCAC CACCCCCTTT
    GAGAACACCT CGATCCGAAC CGGCCCAGCC AGGAGAAGCA GTTATCGATC CAAAATGGCG
    CGGAGGAACA AGCGGATCGC TGGGAGAGAG GGGCAGGAGA GTAAGAAGCG GAGCTCTCTA
    TTCCGTAAGA TCACCAAGCA ATCCAATCTT CTGCACACCA GCCGTTCCCT CTCCTCTCTG
    AGCCGCTCCC TCTCCTCAAG TGACAGTCTT CCAGGATCAC CCACTCACAG CCTCTCGGCC
    CGTTCCCCGA CCCATAGCTA CCGCTCCATG GACTCTGCTT ACCTTGGTGC TTCTTCGCAG
    GGCAGTTCCC CCTCTTCCAG TACCCCTAAT TCCCCTGCAC CACCACCTCC TGCCTCAGCC
    TCTTCCTGTG CGTCAGCCTC CTCCTCCTCC ACCACTCCTG CTCCCCCCTC CCCACACATC
    CGACCCAGCA CCCTGCATGG CTTATCTCCC AAACTGCACC GCCAGTATCG CTCAGCGCGA
    TGTAAATCTG CGGGTAATAT GCCCCTCTCT CCCCTTGCAC ACACCCCTTC CCCTACTGCA
    GCTGCCTCAC CCCCTCTCCT ACCAAGTCAC AGCATTGGCA GCAGCCTGAC CACTCAGAGC
    TTTCCCCCAA AACTGCATCC ATCTCCACCA GTGTCACGCC CTCGCCCAAA GAGCGCAGAG
    CCTCCACGGT CCCCACTTCT TAAGAGAGTG CAGTCTGCAG AGAAACTGGG TGGGGGAGGA
    GGTGGTGGGT CACCCAGAAA ACAGGAGCCG GGAGGGGGAG GAACAATAGA GGGAGATTTA
    GCAGAAGCCA TTGGTGTGAG GAGCCAGCCA GTAAGGAGAG TGGGTAGGCA GGAGTCTCCT
    CGCAGTCTTT GTGGAGGGGA AAATATGATA GGAGAGAACT TTGAGAGGCT GGAAGGAGAA
    GGAGCGATAG GACTATCGCC GCTAGGAGAC AGTCTGAAAT GTAGTGAGGG TAAAGTCCAA
    GCAGCCGTTA AGGTTGGAGT AGGATCAAGG ACTACTCCCC CATTTTCTCC CCAAGAACCT
    CCAGCCGCAT CAACTGCAGT CCTTCCCCCC CAAAGTGACC CTTCCCTTGC TATGGTAGGA
    AGGTGCCAAG TGAAAACCCT AAGTCCTGTA CAAGAACATG AAGGTAGAAG GGGGGAAGGA
    GAGGACGGGG GAGCAGAGGG AGAGGGAAGA AAGGGAGATT ATGAGGAGGA ACAAGGAAGA
    ACAAGAGAAC AATGGTACTT AGAGGTGGTA GAAGAGTTGA CTACAGTAGG GAAAAGCAGC
    ATGCATGCTG GGAGTAAAGC GACTCCCTCT AACCTCACAG CCGACTCTAG TATCTACCAT
    TCCCACTCAA ATAAGAGTAA AGTCGCTGTG GTTGACTCTA TGAAGAGCAC ACCCTCTCAC
    CCTACAGGTA CAACTCTGGA GCAGTCTCTT TCTTTGAAAG GGATTAGATC TGATGCCGAC
    TCTAAAAAGG GGCAAGATCC TAAAAACTGC CAGATTACTC CTCTTCTTCC CGTAAGGGAG
    GGCCTAAATG TTGAACCAAT TCAAAATGGT TCTACTCACC CTCCTGAACT TTCCCACGTG
    GCCAAGGAGC AAAGTATCGA CCATCTAAAA GGTCCATCAT CTTCTGGCCA ACAAATGAAA
    AGCACTAGCA CTTTCCTACC TACTCAGCTT CACCCAACCA CTGGTAGAAA GCTGGAGGCT
    GATAATGTAA GTAGGCATTT ACTCTGTACA GAGGGTACAA AGTATGGTCT GATTAAACCA
    ACAGATTCTC TGTCTATTAG TATTAAAGGG GAGAAGGCCA GTTTAGCAAA AAGCCCCCAG
    GATGATCCAG TAAAGGCCGC TGATCATACT ACTGGGGTGC AAGGACCTGC TCCTGCAAAA
    GGACCAGCAA TTTCACCTGT AAATATTAAA ATCGGCAGAG AAGGATCTGA ACAGACAAGG
    GTGACCACTA CTGACCCAAC TATTAATAGG CTGAGCGAAA CACAAACTAA TCAGAGACCT
    GCAGCTGCCA CTGGTCTTGC TAGCAATATG GTACTTAGGG AGATGGAGAA GAAGGAAGAG
    TTCAGTTCAG CCCAGAATAT AAAGAAACCA AGTGGAGCAG AATTAGAACA GAAGCGGGTG
    GCCATTGTAG TCCCGATTGT AACTAGACCC AGTGATGTAA AGGCTGACAC AACAAGGGTA
    GTATCTGCTC CCCTGACTGT TAATAGACCT GCCGCAGGAG AGAGTGACCA AGCAAAAGTG
    ACCACTGTTA CCCCAACTAT TGATAAAGTC AGTGGCCCAG AAAGTGCCTC TGCCAAGGTG
    ACCGCCGTTG CCTCAATGAG TAGCGCTAAC AAAGCTGTTA ATGAACCACC AAGCCTGTTC
    CCCGATAATA AAGTAGCCGA CTCCAATCAA ATAAGGTTCT CGTCACAGAA TAACAAGGGC
    GCGAGAGAAT CAGAAAGTGA CCAGATAAGG GCAACGGGCA ATATTCCCGT TATTAACCAG
    ACCGCTCAAA CAACGGTTCC TTCCCCAACA CCCACAAATG GCCAAGGACC CCCTATAATT
    ATCCGAGTTA GTGGGGTTCC ATGTGATGCC CAGGGCGGGC TGTGTCCTGA GAAACCTTCC
    AGCCCCCAAA GAGCGCCCAC CACTACCAGC CAACCCCCTT CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55396
    Clone ID Related Accession (Same CDS sequence) XM_031899445.1
    Accession Version XM_031899445.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5685bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product microtubule-associated serine/threonine-protein kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGATGATT CTGGCATTAT CAGACGCAGG AGGCTGCAGG ATCTGCCCCT GCCGAGGAAG 
    AGCAGCGGTT GCAGAAGCAG CAACAGAAAG AGTTTAATCC TCACAAGCAG CAGTTCCCCC
    ACTCTCCCCC GGCCCCACTC CCCCCTGCCG GGGCATATAG GTGGGAGCCC TATGGACAGT
    CCCAGGAACT TCTCCCCCAG CGCCGCTGCT CACTTCTCCT TTGCATCATC TCGCAGCAGA
    ACCGATGGCC GTCGCTGGTC CTTGGCCTCC CTCCCGTCGT CCGGCTATGG GACAAATACC
    CCGAGTTCCA CGGTGTCGTC CTCCTCCTCC TCTCAGGAAC GCCTACACCA GCTCCCCTTC
    CAGCCCACAC TGGATGAGCT CCACTTCCTT TCCAAACACT TTGGCAGCAC AGAAAGTATC
    CCCGATGATG ATGGGGGGCG CAGGTCACCT GCGGTCAGAC CACGATCCCG CAGCTTAAGT
    CCTGGCCGAT CTCCCTCCTC TTATGACAAT GAAATTGTGA TGATGAACCA TGTTTATAAA
    GAGAGGTTTC CCAAGGCCAC AGCGCAGATG GAGGATAGGC TACGGATGTT CATCTCCTCC
    TTCTCCCCCG AGAATGTCCT ACCACTGGCG GACGGACTCC TCAACTTTAT CCACCATCAG
    ATCGTGGAGC TGGCGAGGGA CTGCCTGAAC AGGAGCACCG AGGGCATGAT CACAACCGCC
    CACTTTTACC AACTACAGGA GAATCTGGAG AAGCTGCTGA ATGATGCAGT GGAACGTTCT
    TCAGAGGGAT CAGAAGTTGC CTTCATTGCT CAACTGATGA AAAATCTTTT AATCATCATC
    TCTCGACCTG CTCGTCTGCT GGAGTGTCTG GAATTTAACC CTGAAGAGTT CTATCACATG
    CTGGAGGTAG CCGAGGACCA GGTGAAAGAA GGAAACTTGA AAAAAGACAT ACCCCAATAC
    ATCCTGAGAC AGTTGGGATT AACCAGGGAT TCCTTCCCAG AGGTAGCCGC TCCCGAGGAA
    CCAGTGACCC TGGAAGCACA AACCTCAGAA GCAGAGGACA ACGTAGAGGC AAAGAGTGGG
    GCGGCCCATA AACCCATCAG ACCACCCGAG GAAAGTGACT TTGAAAATAT AAAGCTCATC
    AGTAACGGAG CCTATGGAGC GGTGTATTTG GTGCGTCACC GGGAGACGCG GCAACGCTTT
    GCCATGAAGA AGATCAACAA GCAGAACTTG CGTCTGAGGA AACAAGTCCA CCAGGCCTTT
    GTAGAACGGG ACATTCTGAC CTTTGCTCAG AACCCCTTTG TGGTCAGCAT GTTCTGCTCC
    TTCCAGACCC GTAGGCACCT CTGTATGGTG ATGGAGTATG TGGAAGGGGG TGACTGTGCC
    ACCCTACTGA AGCACATTGG TGCTCTCCCA TTGGAAATGG CGCGGTTATA TTTCGCAGAG
    ACGGTTCTAG CTCTGGAATA CCTACATAAT TATGGGATTG TGCACCGAGA TCTGAAACCG
    GACAATCTCC TAATCACTTC GATGGGTCAC ATAAAGCTTA CAGATTTCGG CCTGTCCAAG
    ATGGGGCTTA TGAACCTCAC CACTAATCTC TATGAAGGAC ACATCGAGAG CGAAGCAAGG
    GAATTCCTGG ACAAGCAGGT CTGTGGAACC CCTGAATACA TCGCACCGGA GGTTATCCTT
    AGACAAGGTT ACGGGAAGCC AGTGGACTGG TGGGCGATGG GCATAATTCT GTATGAGTTC
    CTGGTTGGTT GCGTGCCATT TTTTGGAGAC ACCCCAGAGG AGCTGTTTGG TCAGGTGATC
    AGTGATGACA TTGTGTGGCC AGAAGCAGAG GAAGCTCTAC CCGCCGATGC CCAAAATCTC
    ATCTCCTGCC TCCTGCAGAC AGATCCCTTA CAAAGGCTTG GAACAGGGGG AGCCATTGAG
    GTGAAAATTC ACAGATTTTT CACTGATTTG GACTGGAATG GATTGCTCAG GCAGAAAGCT
    GAATTCATCC CTCACTTGGA GGCAGAAGAT GATACCAGTT ACTTTGACAC TCGATCGGAT
    CGGTACCAGC ACATTAATTC CTACGATGAG GATGATACCA ATGATGAAGA CTCGGTGGAG
    ATCAGGCAGT TCTCATCCTG CACCCCAAGA TTTAGTAAGG TGTATAGTAG TATGGAGCAG
    CTCTCCCAGC TGGAGCAGAA ACCCCCCACT CCTCCTAGAG ACACCAACAA ACAAGAAAGG
    TCTGAGAAGA CGACCAAGAG AGAGACGTTG GGGGGCGTCA CCCTTCGAGA CCGCTGCTGG
    AGGACCGTGT CTCCTGAAAT GAAACAATTC TCCGATGCGG CAGACGCAGA ATCCAGCCCC
    CCCTTGGCTG CCCGGCGGCG CTTTTCTGCC TTAGTGGACC CGACCCGCTA TACGTCTCCC
    CTGGAGTATG AGGTGGAGTA CAGAGTGAGG GGAAGCAGCG GGCCGCTGCC CGCCCAAAGC
    AAGATCCCTC TGGGAGAGCT GGAGCCAGTA GACAAGTCTG TCGACTCTCA GCCCCACCAG
    GAGGCGGATT CCTCTCCTCG CGCCACAAAC GAGATTGTCC TGCGCAGAGC CAGGCACCAA
    CACCTGTCCG GGGACACCGA GAGGAGGAGA ACCGGCGGGA AAGTCATCAA GTCGGCATCG
    ACCACCGCCC TGTCTGTCAT GATCCCTACA GTTGAACAGC ACAGCAGCTC TCCCCTGGTC
    AGCCCCATGT CCCCCCGCTC TCTGTCGTCC AATCCCTCCT CCCGGGATTC CTCTCCGAGC
    CGCGACTACA CGCCGGCAGT GAGCAGCCTC CGATCCCCCA TTTGCATCCA ACGCTCCGGG
    AAAAAATACG GTTTCACCTT GCGGGCCATC CGGGTGTACA TGGGGGAAAG TGATGTGTAC
    AGCGTGCAGC ACATTGTCTG GCACGTGGAG GAAGGAGGGC CTGCCCATGA AGCGGGTCTC
    TGTGCCGGGG ACCTCATTAC ACACATTAAC GGGGAGCCGG TGCACGGCCT GGTGCACACT
    GAAGTTGTGG AGCTGATTTT AAAGAGCGGT AACAAGGTGT TAGTAACCAC CACCCCCTTT
    GAGAACACCT CGATCCGAAC CGGCCCAGCC AGGAGAAGCA GTTATCGATC CAAAATGGCG
    CGGAGGAACA AGCGGATCGC TGGGAGAGAG GGGCAGGAGA GTAAGAAGCG GAGCTCTCTA
    TTCCGTAAGA TCACCAAGCA ATCCAATCTT CTGCACACCA GCCGTTCCCT CTCCTCTCTG
    AGCCGCTCCC TCTCCTCAAG TGACAGTCTT CCAGGATCAC CCACTCACAG CCTCTCGGCC
    CGTTCCCCGA CCCATAGCTA CCGCTCCATG GACTCTGCTT ACCTTGGTGC TTCTTCGCAG
    GGCAGTTCCC CCTCTTCCAG TACCCCTAAT TCCCCTGCAC CACCACCTCC TGCCTCAGCC
    TCTTCCTGTG CGTCAGCCTC CTCCTCCTCC ACCACTCCTG CTCCCCCCTC CCCACACATC
    CGACCCAGCA CCCTGCATGG CTTATCTCCC AAACTGCACC GCCAGTATCG CTCAGCGCGA
    TGTAAATCTG CGGGTAATAT GCCCCTCTCT CCCCTTGCAC ACACCCCTTC CCCTACTGCA
    GCTGCCTCAC CCCCTCTCCT ACCAAGTCAC AGCATTGGCA GCAGCCTGAC CACTCAGAGC
    TTTCCCCCAA AACTGCATCC ATCTCCACCA GTGTCACGCC CTCGCCCAAA GAGCGCAGAG
    CCTCCACGGT CCCCACTTCT TAAGAGAGTG CAGTCTGCAG AGAAACTGGG TGGGGGAGGA
    GGTGGTGGGT CACCCAGAAA ACAGGAGCCG GGAGGGGGAG GAACAATAGA GGGAGATTTA
    GCAGAAGCCA TTGGTGTGAG GAGCCAGCCA GTAAGGAGAG TGGGTAGGCA GGAGTCTCCT
    CGCAGTCTTT GTGGAGGGGA AAATATGATA GGAGAGAACT TTGAGAGGCT GGAAGGAGAA
    GGAGCGATAG GACTATCGCC GCTAGGAGAC AGTCTGAAAT GTAGTGAGGG TAAAGTCCAA
    GCAGCCGTTA AGGTTGGAGT AGGATCAAGG ACTACTCCCC CATTTTCTCC CCAAGAACCT
    CCAGCCGCAT CAACTGCAGT CCTTCCCCCC CAAAGTGACC CTTCCCTTGC TATGGTAGGA
    AGGTGCCAAG TGAAAACCCT AAGTCCTGTA CAAGAACATG AAGGTAGAAG GGGGGAAGGA
    GAGGACGGGG GAGCAGAGGG AGAGGGAAGA AAGGGAGATT ATGAGGAGGA ACAAGGAAGA
    ACAAGAGAAC AATGGTACTT AGAGGTGGTA GAAGAGTTGA CTACAGTAGG GAAAAGCAGC
    ATGCATGCTG GGAGTAAAGC GACTCCCTCT AACCTCACAG CCGACTCTAG TATCTACCAT
    TCCCACTCAA ATAAGAGTAA AGTCGCTGTG GTTGACTCTA TGAAGAGCAC ACCCTCTCAC
    CCTACAGGTA CAACTCTGGA GCAGTCTCTT TCTTTGAAAG GGATTAGATC TGATGCCGAC
    TCTAAAAAGG GGCAAGATCC TAAAAACTGC CAGATTACTC CTCTTCTTCC CGTAAGGGAG
    GGCCTAAATG TTGAACCAAT TCAAAATGGT TCTACTCACC CTCCTGAACT TTCCCACGTG
    GCCAAGGAGC AAAGTATCGA CCATCTAAAA GGTCCATCAT CTTCTGGCCA ACAAATGAAA
    AGCACTAGCA CTTTCCTACC TACTCAGCTT CACCCAACCA CTGGTAGAAA GCTGGAGGCT
    GATAATGTAA GTAGGCATTT ACTCTGTACA GAGGGTACAA AGTATGGTCT GATTAAACCA
    ACAGATTCTC TGTCTATTAG TATTAAAGGG GAGAAGGCCA GTTTAGCAAA AAGCCCCCAG
    GATGATCCAG TAAAGGCCGC TGATCATACT ACTGGGGTGC AAGGACCTGC TCCTGCAAAA
    GGACCAGCAA TTTCACCTGT AAATATTAAA ATCGGCAGAG AAGGATCTGA ACAGACAAGG
    GTGACCACTA CTGACCCAAC TATTAATAGG CTGAGCGAAA CACAAACTAA TCAGAGACCT
    GCAGCTGCCA CTGGTCTTGC TAGCAATATG GTACTTAGGG AGATGGAGAA GAAGGAAGAG
    TTCAGTTCAG CCCAGAATAT AAAGAAACCA AGTGGAGCAG AATTAGAACA GAAGCGGGTG
    GCCATTGTAG TCCCGATTGT AACTAGACCC AGTGATGTAA AGGCTGACAC AACAAGGGTA
    GTATCTGCTC CCCTGACTGT TAATAGACCT GCCGCAGGAG AGAGTGACCA AGCAAAAGTG
    ACCACTGTTA CCCCAACTAT TGATAAAGTC AGTGGCCCAG AAAGTGCCTC TGCCAAGGTG
    ACCGCCGTTG CCTCAATGAG TAGCGCTAAC AAAGCTGTTA ATGAACCACC AAGCCTGTTC
    CCCGATAATA AAGTAGCCGA CTCCAATCAA ATAAGGTTCT CGTCACAGAA TAACAAGGGC
    GCGAGAGAAT CAGAAAGTGA CCAGATAAGG GCAACGGGCA ATATTCCCGT TATTAACCAG
    ACCGCTCAAA CAACGGTTCC TTCCCCAACA CCCACAAATG GCCAAGGACC CCCTATAATT
    ATCCGAGTTA GTGGGGTTCC ATGTGATGCC CAGGGCGGGC TGTGTCCTGA GAAACCTTCC
    AGCCCCCAAA GAGCGCCCAC CACTACCAGC CAACCCCCTT CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755305.1
    CDS67..5751
    Translation

    Target ORF information:

    RefSeq Version XM_031899445.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis microtubule associated serine/threonine kinase 1 (mast1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899445.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGATGATT CTGGCATTAT CAGACGCAGG AGGCTGCAGG ATCTGCCCCT GCCGAGGAAG 
    AGCAGCGGTT GCAGAAGCAG CAACAGAAAG AGTTTAATCC TCACAAGCAG CAGTTCCCCC
    ACTCTCCCCC GGCCCCACTC CCCCCTGCCG GGGCATATAG GTGGGAGCCC TATGGACAGT
    CCCAGGAACT TCTCCCCCAG CGCCGCTGCT CACTTCTCCT TTGCATCATC TCGCAGCAGA
    ACCGATGGCC GTCGCTGGTC CTTGGCCTCC CTCCCGTCGT CCGGCTATGG GACAAATACC
    CCGAGTTCCA CGGTGTCGTC CTCCTCCTCC TCTCAGGAAC GCCTACACCA GCTCCCCTTC
    CAGCCCACAC TGGATGAGCT CCACTTCCTT TCCAAACACT TTGGCAGCAC AGAAAGTATC
    CCCGATGATG ATGGGGGGCG CAGGTCACCT GCGGTCAGAC CACGATCCCG CAGCTTAAGT
    CCTGGCCGAT CTCCCTCCTC TTATGACAAT GAAATTGTGA TGATGAACCA TGTTTATAAA
    GAGAGGTTTC CCAAGGCCAC AGCGCAGATG GAGGATAGGC TACGGATGTT CATCTCCTCC
    TTCTCCCCCG AGAATGTCCT ACCACTGGCG GACGGACTCC TCAACTTTAT CCACCATCAG
    ATCGTGGAGC TGGCGAGGGA CTGCCTGAAC AGGAGCACCG AGGGCATGAT CACAACCGCC
    CACTTTTACC AACTACAGGA GAATCTGGAG AAGCTGCTGA ATGATGCAGT GGAACGTTCT
    TCAGAGGGAT CAGAAGTTGC CTTCATTGCT CAACTGATGA AAAATCTTTT AATCATCATC
    TCTCGACCTG CTCGTCTGCT GGAGTGTCTG GAATTTAACC CTGAAGAGTT CTATCACATG
    CTGGAGGTAG CCGAGGACCA GGTGAAAGAA GGAAACTTGA AAAAAGACAT ACCCCAATAC
    ATCCTGAGAC AGTTGGGATT AACCAGGGAT TCCTTCCCAG AGGTAGCCGC TCCCGAGGAA
    CCAGTGACCC TGGAAGCACA AACCTCAGAA GCAGAGGACA ACGTAGAGGC AAAGAGTGGG
    GCGGCCCATA AACCCATCAG ACCACCCGAG GAAAGTGACT TTGAAAATAT AAAGCTCATC
    AGTAACGGAG CCTATGGAGC GGTGTATTTG GTGCGTCACC GGGAGACGCG GCAACGCTTT
    GCCATGAAGA AGATCAACAA GCAGAACTTG CGTCTGAGGA AACAAGTCCA CCAGGCCTTT
    GTAGAACGGG ACATTCTGAC CTTTGCTCAG AACCCCTTTG TGGTCAGCAT GTTCTGCTCC
    TTCCAGACCC GTAGGCACCT CTGTATGGTG ATGGAGTATG TGGAAGGGGG TGACTGTGCC
    ACCCTACTGA AGCACATTGG TGCTCTCCCA TTGGAAATGG CGCGGTTATA TTTCGCAGAG
    ACGGTTCTAG CTCTGGAATA CCTACATAAT TATGGGATTG TGCACCGAGA TCTGAAACCG
    GACAATCTCC TAATCACTTC GATGGGTCAC ATAAAGCTTA CAGATTTCGG CCTGTCCAAG
    ATGGGGCTTA TGAACCTCAC CACTAATCTC TATGAAGGAC ACATCGAGAG CGAAGCAAGG
    GAATTCCTGG ACAAGCAGGT CTGTGGAACC CCTGAATACA TCGCACCGGA GGTTATCCTT
    AGACAAGGTT ACGGGAAGCC AGTGGACTGG TGGGCGATGG GCATAATTCT GTATGAGTTC
    CTGGTTGGTT GCGTGCCATT TTTTGGAGAC ACCCCAGAGG AGCTGTTTGG TCAGGTGATC
    AGTGATGACA TTGTGTGGCC AGAAGCAGAG GAAGCTCTAC CCGCCGATGC CCAAAATCTC
    ATCTCCTGCC TCCTGCAGAC AGATCCCTTA CAAAGGCTTG GAACAGGGGG AGCCATTGAG
    GTGAAAATTC ACAGATTTTT CACTGATTTG GACTGGAATG GATTGCTCAG GCAGAAAGCT
    GAATTCATCC CTCACTTGGA GGCAGAAGAT GATACCAGTT ACTTTGACAC TCGATCGGAT
    CGGTACCAGC ACATTAATTC CTACGATGAG GATGATACCA ATGATGAAGA CTCGGTGGAG
    ATCAGGCAGT TCTCATCCTG CACCCCAAGA TTTAGTAAGG TGTATAGTAG TATGGAGCAG
    CTCTCCCAGC TGGAGCAGAA ACCCCCCACT CCTCCTAGAG ACACCAACAA ACAAGAAAGG
    TCTGAGAAGA CGACCAAGAG AGAGACGTTG GGGGGCGTCA CCCTTCGAGA CCGCTGCTGG
    AGGACCGTGT CTCCTGAAAT GAAACAATTC TCCGATGCGG CAGACGCAGA ATCCAGCCCC
    CCCTTGGCTG CCCGGCGGCG CTTTTCTGCC TTAGTGGACC CGACCCGCTA TACGTCTCCC
    CTGGAGTATG AGGTGGAGTA CAGAGTGAGG GGAAGCAGCG GGCCGCTGCC CGCCCAAAGC
    AAGATCCCTC TGGGAGAGCT GGAGCCAGTA GACAAGTCTG TCGACTCTCA GCCCCACCAG
    GAGGCGGATT CCTCTCCTCG CGCCACAAAC GAGATTGTCC TGCGCAGAGC CAGGCACCAA
    CACCTGTCCG GGGACACCGA GAGGAGGAGA ACCGGCGGGA AAGTCATCAA GTCGGCATCG
    ACCACCGCCC TGTCTGTCAT GATCCCTACA GTTGAACAGC ACAGCAGCTC TCCCCTGGTC
    AGCCCCATGT CCCCCCGCTC TCTGTCGTCC AATCCCTCCT CCCGGGATTC CTCTCCGAGC
    CGCGACTACA CGCCGGCAGT GAGCAGCCTC CGATCCCCCA TTTGCATCCA ACGCTCCGGG
    AAAAAATACG GTTTCACCTT GCGGGCCATC CGGGTGTACA TGGGGGAAAG TGATGTGTAC
    AGCGTGCAGC ACATTGTCTG GCACGTGGAG GAAGGAGGGC CTGCCCATGA AGCGGGTCTC
    TGTGCCGGGG ACCTCATTAC ACACATTAAC GGGGAGCCGG TGCACGGCCT GGTGCACACT
    GAAGTTGTGG AGCTGATTTT AAAGAGCGGT AACAAGGTGT TAGTAACCAC CACCCCCTTT
    GAGAACACCT CGATCCGAAC CGGCCCAGCC AGGAGAAGCA GTTATCGATC CAAAATGGCG
    CGGAGGAACA AGCGGATCGC TGGGAGAGAG GGGCAGGAGA GTAAGAAGCG GAGCTCTCTA
    TTCCGTAAGA TCACCAAGCA ATCCAATCTT CTGCACACCA GCCGTTCCCT CTCCTCTCTG
    AGCCGCTCCC TCTCCTCAAG TGACAGTCTT CCAGGATCAC CCACTCACAG CCTCTCGGCC
    CGTTCCCCGA CCCATAGCTA CCGCTCCATG GACTCTGCTT ACCTTGGTGC TTCTTCGCAG
    GGCAGTTCCC CCTCTTCCAG TACCCCTAAT TCCCCTGCAC CACCACCTCC TGCCTCAGCC
    TCTTCCTGTG CGTCAGCCTC CTCCTCCTCC ACCACTCCTG CTCCCCCCTC CCCACACATC
    CGACCCAGCA CCCTGCATGG CTTATCTCCC AAACTGCACC GCCAGTATCG CTCAGCGCGA
    TGTAAATCTG CGGGTAATAT GCCCCTCTCT CCCCTTGCAC ACACCCCTTC CCCTACTGCA
    GCTGCCTCAC CCCCTCTCCT ACCAAGTCAC AGCATTGGCA GCAGCCTGAC CACTCAGAGC
    TTTCCCCCAA AACTGCATCC ATCTCCACCA GTGTCACGCC CTCGCCCAAA GAGCGCAGAG
    CCTCCACGGT CCCCACTTCT TAAGAGAGTG CAGTCTGCAG AGAAACTGGG TGGGGGAGGA
    GGTGGTGGGT CACCCAGAAA ACAGGAGCCG GGAGGGGGAG GAACAATAGA GGGAGATTTA
    GCAGAAGCCA TTGGTGTGAG GAGCCAGCCA GTAAGGAGAG TGGGTAGGCA GGAGTCTCCT
    CGCAGTCTTT GTGGAGGGGA AAATATGATA GGAGAGAACT TTGAGAGGCT GGAAGGAGAA
    GGAGCGATAG GACTATCGCC GCTAGGAGAC AGTCTGAAAT GTAGTGAGGG TAAAGTCCAA
    GCAGCCGTTA AGGTTGGAGT AGGATCAAGG ACTACTCCCC CATTTTCTCC CCAAGAACCT
    CCAGCCGCAT CAACTGCAGT CCTTCCCCCC CAAAGTGACC CTTCCCTTGC TATGGTAGGA
    AGGTGCCAAG TGAAAACCCT AAGTCCTGTA CAAGAACATG AAGGTAGAAG GGGGGAAGGA
    GAGGACGGGG GAGCAGAGGG AGAGGGAAGA AAGGGAGATT ATGAGGAGGA ACAAGGAAGA
    ACAAGAGAAC AATGGTACTT AGAGGTGGTA GAAGAGTTGA CTACAGTAGG GAAAAGCAGC
    ATGCATGCTG GGAGTAAAGC GACTCCCTCT AACCTCACAG CCGACTCTAG TATCTACCAT
    TCCCACTCAA ATAAGAGTAA AGTCGCTGTG GTTGACTCTA TGAAGAGCAC ACCCTCTCAC
    CCTACAGGTA CAACTCTGGA GCAGTCTCTT TCTTTGAAAG GGATTAGATC TGATGCCGAC
    TCTAAAAAGG GGCAAGATCC TAAAAACTGC CAGATTACTC CTCTTCTTCC CGTAAGGGAG
    GGCCTAAATG TTGAACCAAT TCAAAATGGT TCTACTCACC CTCCTGAACT TTCCCACGTG
    GCCAAGGAGC AAAGTATCGA CCATCTAAAA GGTCCATCAT CTTCTGGCCA ACAAATGAAA
    AGCACTAGCA CTTTCCTACC TACTCAGCTT CACCCAACCA CTGGTAGAAA GCTGGAGGCT
    GATAATGTAA GTAGGCATTT ACTCTGTACA GAGGGTACAA AGTATGGTCT GATTAAACCA
    ACAGATTCTC TGTCTATTAG TATTAAAGGG GAGAAGGCCA GTTTAGCAAA AAGCCCCCAG
    GATGATCCAG TAAAGGCCGC TGATCATACT ACTGGGGTGC AAGGACCTGC TCCTGCAAAA
    GGACCAGCAA TTTCACCTGT AAATATTAAA ATCGGCAGAG AAGGATCTGA ACAGACAAGG
    GTGACCACTA CTGACCCAAC TATTAATAGG CTGAGCGAAA CACAAACTAA TCAGAGACCT
    GCAGCTGCCA CTGGTCTTGC TAGCAATATG GTACTTAGGG AGATGGAGAA GAAGGAAGAG
    TTCAGTTCAG CCCAGAATAT AAAGAAACCA AGTGGAGCAG AATTAGAACA GAAGCGGGTG
    GCCATTGTAG TCCCGATTGT AACTAGACCC AGTGATGTAA AGGCTGACAC AACAAGGGTA
    GTATCTGCTC CCCTGACTGT TAATAGACCT GCCGCAGGAG AGAGTGACCA AGCAAAAGTG
    ACCACTGTTA CCCCAACTAT TGATAAAGTC AGTGGCCCAG AAAGTGCCTC TGCCAAGGTG
    ACCGCCGTTG CCTCAATGAG TAGCGCTAAC AAAGCTGTTA ATGAACCACC AAGCCTGTTC
    CCCGATAATA AAGTAGCCGA CTCCAATCAA ATAAGGTTCT CGTCACAGAA TAACAAGGGC
    GCGAGAGAAT CAGAAAGTGA CCAGATAAGG GCAACGGGCA ATATTCCCGT TATTAACCAG
    ACCGCTCAAA CAACGGTTCC TTCCCCAACA CCCACAAATG GCCAAGGACC CCCTATAATT
    ATCCGAGTTA GTGGGGTTCC ATGTGATGCC CAGGGCGGGC TGTGTCCTGA GAAACCTTCC
    AGCCCCCAAA GAGCGCCCAC CACTACCAGC CAACCCCCTT CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55397
    Clone ID Related Accession (Same CDS sequence) XM_031899446.1
    Accession Version XM_031899446.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5685bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product microtubule-associated serine/threonine-protein kinase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGATGATT CTGGCATTAT CAGACGCAGG AGGCTGCAGA AGGATCTGCC CCTGCCGAGG 
    AAGAGCAGCG GTTGCAGAAG CAGCAACAGA AAGAGTTTAA TCCTCACAAG CAGCAGTTCC
    CCCACTCTCC CCCGGCCCCA CTCCCCCCTG CCGGGGCATA TAGGTGGGAG CCCTATGGAC
    AGTCCCAGGA ACTTCTCCCC CAGCGCCGCT GCTCACTTCT CCTTTGCATC ATCTCGCAGC
    AGAACCGATG GCCGTCGCTG GTCCTTGGCC TCCCTCCCGT CGTCCGGCTA TGGGACAAAT
    ACCCCGAGTT CCACGGTGTC GTCCTCCTCC TCCTCTCAGG AACGCCTACA CCAGCTCCCC
    TTCCAGCCCA CACTGGATGA GCTCCACTTC CTTTCCAAAC ACTTTGGCAG CACAGAAAGT
    ATCCCCGATG ATGATGGGGG GCGCAGGTCA CCTGCGGTCA GACCACGATC CCGCAGCTTA
    AGTCCTGGCC GATCTCCCTC CTCTTATGAC AATGAAATTG TGATGATGAA CCATGTTTAT
    AAAGAGAGGT TTCCCAAGGC CACAGCGCAG ATGGAGGATA GGCTACGGAT GTTCATCTCC
    TCCTTCTCCC CCGAGAATGT CCTACCACTG GCGGACGGAC TCCTCAACTT TATCCACCAT
    CAGATCGTGG AGCTGGCGAG GGACTGCCTG AACAGGAGCA CCGAGGGCAT GATCACAACC
    GCCCACTTTT ACCAACTACA GGAGAATCTG GAGAAGCTGC TGAATGATGC AGTGGAACGT
    TCTTCAGAGG GATCAGAAGT TGCCTTCATT GCTCAACTGA TGAAAAATCT TTTAATCATC
    ATCTCTCGAC CTGCTCGTCT GCTGGAGTGT CTGGAATTTA ACCCTGAAGA GTTCTATCAC
    ATGCTGGAGG TAGCCGAGGA CCAGGTGAAA GAAGGAAACT TGAAAAAAGA CATACCCCAA
    TACATCCTGA GACAGTTGGG ATTAACCAGG GATTCCTTCC CAGAGGTAGC CGCTCCCGAG
    GAACCAGTGA CCCTGGAAGC ACAAACCTCA GAAGCAGAGG ACAACGTAGA GGCAAAGAGT
    GGGGCGGCCC ATAAACCCAT CAGACCACCC GAGGAAAGTG ACTTTGAAAA TATAAAGCTC
    ATCAGTAACG GAGCCTATGG AGCGGTGTAT TTGGTGCGTC ACCGGGAGAC GCGGCAACGC
    TTTGCCATGA AGAAGATCAA CAAGCAGAAC TTGCGTCTGA GGAAACAAGT CCACCAGGCC
    TTTGTAGAAC GGGACATTCT GACCTTTGCT CAGAACCCCT TTGTGGTCAG CATGTTCTGC
    TCCTTCCAGA CCCGTAGGCA CCTCTGTATG GTGATGGAGT ATGTGGAAGG GGGTGACTGT
    GCCACCCTAC TGAAGCACAT TGGTGCTCTC CCATTGGAAA TGGCGCGGTT ATATTTCGCA
    GAGACGGTTC TAGCTCTGGA ATACCTACAT AATTATGGGA TTGTGCACCG AGATCTGAAA
    CCGGACAATC TCCTAATCAC TTCGATGGGT CACATAAAGC TTACAGATTT CGGCCTGTCC
    AAGATGGGGC TTATGAACCT CACCACTAAT CTCTATGAAG GACACATCGA GAGCGAAGCA
    AGGGAATTCC TGGACAAGCA GGTCTGTGGA ACCCCTGAAT ACATCGCACC GGAGGTTATC
    CTTAGACAAG GTTACGGGAA GCCAGTGGAC TGGTGGGCGA TGGGCATAAT TCTGTATGAG
    TTCCTGGTTG GTTGCGTGCC ATTTTTTGGA GACACCCCAG AGGAGCTGTT TGGTCAGGTG
    ATCAGTGATG ACATTGTGTG GCCAGAAGCA GAGGAAGCTC TACCCGCCGA TGCCCAAAAT
    CTCATCTCCT GCCTCCTGCA GACAGATCCC TTACAAAGGC TTGGAACAGG GGGAGCCATT
    GAGGTGAAAA TTCACAGATT TTTCACTGAT TTGGACTGGA ATGGATTGCT CAGGCAGAAA
    GCTGAATTCA TCCCTCACTT GGAGGCAGAA GATGATACCA GTTACTTTGA CACTCGATCG
    GATCGGTACC AGCACATTAA TTCCTACGAT GAGGATGATA CCAATGATGA AGACTCGGTG
    GAGATCAGGC AGTTCTCATC CTGCACCCCA AGATTTAGTA AGGTGTATAG TAGTATGGAG
    CAGCTCTCCC AGCTGGAGCA GAAACCCCCC ACTCCTCCTA GAGACACCAA CAAACAAGAA
    AGGTCTGAGA AGACGACCAA GAGAGAGACG TTGGGGGGCG TCACCCTTCG AGACCGCTGC
    TGGAGGACCG TGTCTCCTGA AATGAAACAA TTCTCCGATG CGGCAGACGC AGAATCCAGC
    CCCCCCTTGG CTGCCCGGCG GCGCTTTTCT GCCTTAGTGG ACCCGACCCG CTATACGTCT
    CCCCTGGAGT ATGAGGTGGA GTACAGAGTG AGGGGAAGCA GCGGGCCGCT GCCCGCCCAA
    AGCAAGATCC CTCTGGGAGA GCTGGAGCCA GACAAGTCTG TCGACTCTCA GCCCCACCAG
    GAGGCGGATT CCTCTCCTCG CGCCACAAAC GAGATTGTCC TGCGCAGAGC CAGGCACCAA
    CACCTGTCCG GGGACACCGA GAGGAGGAGA ACCGGCGGGA AAGTCATCAA GTCGGCATCG
    ACCACCGCCC TGTCTGTCAT GATCCCTACA GTTGAACAGC ACAGCAGCTC TCCCCTGGTC
    AGCCCCATGT CCCCCCGCTC TCTGTCGTCC AATCCCTCCT CCCGGGATTC CTCTCCGAGC
    CGCGACTACA CGCCGGCAGT GAGCAGCCTC CGATCCCCCA TTTGCATCCA ACGCTCCGGG
    AAAAAATACG GTTTCACCTT GCGGGCCATC CGGGTGTACA TGGGGGAAAG TGATGTGTAC
    AGCGTGCAGC ACATTGTCTG GCACGTGGAG GAAGGAGGGC CTGCCCATGA AGCGGGTCTC
    TGTGCCGGGG ACCTCATTAC ACACATTAAC GGGGAGCCGG TGCACGGCCT GGTGCACACT
    GAAGTTGTGG AGCTGATTTT AAAGAGCGGT AACAAGGTGT TAGTAACCAC CACCCCCTTT
    GAGAACACCT CGATCCGAAC CGGCCCAGCC AGGAGAAGCA GTTATCGATC CAAAATGGCG
    CGGAGGAACA AGCGGATCGC TGGGAGAGAG GGGCAGGAGA GTAAGAAGCG GAGCTCTCTA
    TTCCGTAAGA TCACCAAGCA ATCCAATCTT CTGCACACCA GCCGTTCCCT CTCCTCTCTG
    AGCCGCTCCC TCTCCTCAAG TGACAGTCTT CCAGGATCAC CCACTCACAG CCTCTCGGCC
    CGTTCCCCGA CCCATAGCTA CCGCTCCATG GACTCTGCTT ACCTTGGTGC TTCTTCGCAG
    GGCAGTTCCC CCTCTTCCAG TACCCCTAAT TCCCCTGCAC CACCACCTCC TGCCTCAGCC
    TCTTCCTGTG CGTCAGCCTC CTCCTCCTCC ACCACTCCTG CTCCCCCCTC CCCACACATC
    CGACCCAGCA CCCTGCATGG CTTATCTCCC AAACTGCACC GCCAGTATCG CTCAGCGCGA
    TGTAAATCTG CGGGTAATAT GCCCCTCTCT CCCCTTGCAC ACACCCCTTC CCCTACTGCA
    GCTGCCTCAC CCCCTCTCCT ACCAAGTCAC AGCATTGGCA GCAGCCTGAC CACTCAGAGC
    TTTCCCCCAA AACTGCATCC ATCTCCACCA GTGTCACGCC CTCGCCCAAA GAGCGCAGAG
    CCTCCACGGT CCCCACTTCT TAAGAGAGTG CAGTCTGCAG AGAAACTGGG TGGGGGAGGA
    GGTGGTGGGT CACCCAGAAA ACAGGAGCCG GGAGGGGGAG GAACAATAGA GGGAGATTTA
    GCAGAAGCCA TTGGTGTGAG GAGCCAGCCA GTAAGGAGAG TGGGTAGGCA GGAGTCTCCT
    CGCAGTCTTT GTGGAGGGGA AAATATGATA GGAGAGAACT TTGAGAGGCT GGAAGGAGAA
    GGAGCGATAG GACTATCGCC GCTAGGAGAC AGTCTGAAAT GTAGTGAGGG TAAAGTCCAA
    GCAGCCGTTA AGGTTGGAGT AGGATCAAGG ACTACTCCCC CATTTTCTCC CCAAGAACCT
    CCAGCCGCAT CAACTGCAGT CCTTCCCCCC CAAAGTGACC CTTCCCTTGC TATGGTAGGA
    AGGTGCCAAG TGAAAACCCT AAGTCCTGTA CAAGAACATG AAGGTAGAAG GGGGGAAGGA
    GAGGACGGGG GAGCAGAGGG AGAGGGAAGA AAGGGAGATT ATGAGGAGGA ACAAGGAAGA
    ACAAGAGAAC AATGGTACTT AGAGGTGGTA GAAGAGTTGA CTACAGTAGG GAAAAGCAGC
    ATGCATGCTG GGAGTAAAGC GACTCCCTCT AACCTCACAG CCGACTCTAG TATCTACCAT
    TCCCACTCAA ATAAGAGTAA AGTCGCTGTG GTTGACTCTA TGAAGAGCAC ACCCTCTCAC
    CCTACAGGTA CAACTCTGGA GCAGTCTCTT TCTTTGAAAG GGATTAGATC TGATGCCGAC
    TCTAAAAAGG GGCAAGATCC TAAAAACTGC CAGATTACTC CTCTTCTTCC CGTAAGGGAG
    GGCCTAAATG TTGAACCAAT TCAAAATGGT TCTACTCACC CTCCTGAACT TTCCCACGTG
    GCCAAGGAGC AAAGTATCGA CCATCTAAAA GGTCCATCAT CTTCTGGCCA ACAAATGAAA
    AGCACTAGCA CTTTCCTACC TACTCAGCTT CACCCAACCA CTGGTAGAAA GCTGGAGGCT
    GATAATGTAA GTAGGCATTT ACTCTGTACA GAGGGTACAA AGTATGGTCT GATTAAACCA
    ACAGATTCTC TGTCTATTAG TATTAAAGGG GAGAAGGCCA GTTTAGCAAA AAGCCCCCAG
    GATGATCCAG TAAAGGCCGC TGATCATACT ACTGGGGTGC AAGGACCTGC TCCTGCAAAA
    GGACCAGCAA TTTCACCTGT AAATATTAAA ATCGGCAGAG AAGGATCTGA ACAGACAAGG
    GTGACCACTA CTGACCCAAC TATTAATAGG CTGAGCGAAA CACAAACTAA TCAGAGACCT
    GCAGCTGCCA CTGGTCTTGC TAGCAATATG GTACTTAGGG AGATGGAGAA GAAGGAAGAG
    TTCAGTTCAG CCCAGAATAT AAAGAAACCA AGTGGAGCAG AATTAGAACA GAAGCGGGTG
    GCCATTGTAG TCCCGATTGT AACTAGACCC AGTGATGTAA AGGCTGACAC AACAAGGGTA
    GTATCTGCTC CCCTGACTGT TAATAGACCT GCCGCAGGAG AGAGTGACCA AGCAAAAGTG
    ACCACTGTTA CCCCAACTAT TGATAAAGTC AGTGGCCCAG AAAGTGCCTC TGCCAAGGTG
    ACCGCCGTTG CCTCAATGAG TAGCGCTAAC AAAGCTGTTA ATGAACCACC AAGCCTGTTC
    CCCGATAATA AAGTAGCCGA CTCCAATCAA ATAAGGTTCT CGTCACAGAA TAACAAGGGC
    GCGAGAGAAT CAGAAAGTGA CCAGATAAGG GCAACGGGCA ATATTCCCGT TATTAACCAG
    ACCGCTCAAA CAACGGTTCC TTCCCCAACA CCCACAAATG GCCAAGGACC CCCTATAATT
    ATCCGAGTTA GTGGGGTTCC ATGTGATGCC CAGGGCGGGC TGTGTCCTGA GAAACCTTCC
    AGCCCCCAAA GAGCGCCCAC CACTACCAGC CAACCCCCTT CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755306.1
    CDS65..5749
    Translation

    Target ORF information:

    RefSeq Version XM_031899446.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis microtubule associated serine/threonine kinase 1 (mast1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899446.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGATGATT CTGGCATTAT CAGACGCAGG AGGCTGCAGA AGGATCTGCC CCTGCCGAGG 
    AAGAGCAGCG GTTGCAGAAG CAGCAACAGA AAGAGTTTAA TCCTCACAAG CAGCAGTTCC
    CCCACTCTCC CCCGGCCCCA CTCCCCCCTG CCGGGGCATA TAGGTGGGAG CCCTATGGAC
    AGTCCCAGGA ACTTCTCCCC CAGCGCCGCT GCTCACTTCT CCTTTGCATC ATCTCGCAGC
    AGAACCGATG GCCGTCGCTG GTCCTTGGCC TCCCTCCCGT CGTCCGGCTA TGGGACAAAT
    ACCCCGAGTT CCACGGTGTC GTCCTCCTCC TCCTCTCAGG AACGCCTACA CCAGCTCCCC
    TTCCAGCCCA CACTGGATGA GCTCCACTTC CTTTCCAAAC ACTTTGGCAG CACAGAAAGT
    ATCCCCGATG ATGATGGGGG GCGCAGGTCA CCTGCGGTCA GACCACGATC CCGCAGCTTA
    AGTCCTGGCC GATCTCCCTC CTCTTATGAC AATGAAATTG TGATGATGAA CCATGTTTAT
    AAAGAGAGGT TTCCCAAGGC CACAGCGCAG ATGGAGGATA GGCTACGGAT GTTCATCTCC
    TCCTTCTCCC CCGAGAATGT CCTACCACTG GCGGACGGAC TCCTCAACTT TATCCACCAT
    CAGATCGTGG AGCTGGCGAG GGACTGCCTG AACAGGAGCA CCGAGGGCAT GATCACAACC
    GCCCACTTTT ACCAACTACA GGAGAATCTG GAGAAGCTGC TGAATGATGC AGTGGAACGT
    TCTTCAGAGG GATCAGAAGT TGCCTTCATT GCTCAACTGA TGAAAAATCT TTTAATCATC
    ATCTCTCGAC CTGCTCGTCT GCTGGAGTGT CTGGAATTTA ACCCTGAAGA GTTCTATCAC
    ATGCTGGAGG TAGCCGAGGA CCAGGTGAAA GAAGGAAACT TGAAAAAAGA CATACCCCAA
    TACATCCTGA GACAGTTGGG ATTAACCAGG GATTCCTTCC CAGAGGTAGC CGCTCCCGAG
    GAACCAGTGA CCCTGGAAGC ACAAACCTCA GAAGCAGAGG ACAACGTAGA GGCAAAGAGT
    GGGGCGGCCC ATAAACCCAT CAGACCACCC GAGGAAAGTG ACTTTGAAAA TATAAAGCTC
    ATCAGTAACG GAGCCTATGG AGCGGTGTAT TTGGTGCGTC ACCGGGAGAC GCGGCAACGC
    TTTGCCATGA AGAAGATCAA CAAGCAGAAC TTGCGTCTGA GGAAACAAGT CCACCAGGCC
    TTTGTAGAAC GGGACATTCT GACCTTTGCT CAGAACCCCT TTGTGGTCAG CATGTTCTGC
    TCCTTCCAGA CCCGTAGGCA CCTCTGTATG GTGATGGAGT ATGTGGAAGG GGGTGACTGT
    GCCACCCTAC TGAAGCACAT TGGTGCTCTC CCATTGGAAA TGGCGCGGTT ATATTTCGCA
    GAGACGGTTC TAGCTCTGGA ATACCTACAT AATTATGGGA TTGTGCACCG AGATCTGAAA
    CCGGACAATC TCCTAATCAC TTCGATGGGT CACATAAAGC TTACAGATTT CGGCCTGTCC
    AAGATGGGGC TTATGAACCT CACCACTAAT CTCTATGAAG GACACATCGA GAGCGAAGCA
    AGGGAATTCC TGGACAAGCA GGTCTGTGGA ACCCCTGAAT ACATCGCACC GGAGGTTATC
    CTTAGACAAG GTTACGGGAA GCCAGTGGAC TGGTGGGCGA TGGGCATAAT TCTGTATGAG
    TTCCTGGTTG GTTGCGTGCC ATTTTTTGGA GACACCCCAG AGGAGCTGTT TGGTCAGGTG
    ATCAGTGATG ACATTGTGTG GCCAGAAGCA GAGGAAGCTC TACCCGCCGA TGCCCAAAAT
    CTCATCTCCT GCCTCCTGCA GACAGATCCC TTACAAAGGC TTGGAACAGG GGGAGCCATT
    GAGGTGAAAA TTCACAGATT TTTCACTGAT TTGGACTGGA ATGGATTGCT CAGGCAGAAA
    GCTGAATTCA TCCCTCACTT GGAGGCAGAA GATGATACCA GTTACTTTGA CACTCGATCG
    GATCGGTACC AGCACATTAA TTCCTACGAT GAGGATGATA CCAATGATGA AGACTCGGTG
    GAGATCAGGC AGTTCTCATC CTGCACCCCA AGATTTAGTA AGGTGTATAG TAGTATGGAG
    CAGCTCTCCC AGCTGGAGCA GAAACCCCCC ACTCCTCCTA GAGACACCAA CAAACAAGAA
    AGGTCTGAGA AGACGACCAA GAGAGAGACG TTGGGGGGCG TCACCCTTCG AGACCGCTGC
    TGGAGGACCG TGTCTCCTGA AATGAAACAA TTCTCCGATG CGGCAGACGC AGAATCCAGC
    CCCCCCTTGG CTGCCCGGCG GCGCTTTTCT GCCTTAGTGG ACCCGACCCG CTATACGTCT
    CCCCTGGAGT ATGAGGTGGA GTACAGAGTG AGGGGAAGCA GCGGGCCGCT GCCCGCCCAA
    AGCAAGATCC CTCTGGGAGA GCTGGAGCCA GACAAGTCTG TCGACTCTCA GCCCCACCAG
    GAGGCGGATT CCTCTCCTCG CGCCACAAAC GAGATTGTCC TGCGCAGAGC CAGGCACCAA
    CACCTGTCCG GGGACACCGA GAGGAGGAGA ACCGGCGGGA AAGTCATCAA GTCGGCATCG
    ACCACCGCCC TGTCTGTCAT GATCCCTACA GTTGAACAGC ACAGCAGCTC TCCCCTGGTC
    AGCCCCATGT CCCCCCGCTC TCTGTCGTCC AATCCCTCCT CCCGGGATTC CTCTCCGAGC
    CGCGACTACA CGCCGGCAGT GAGCAGCCTC CGATCCCCCA TTTGCATCCA ACGCTCCGGG
    AAAAAATACG GTTTCACCTT GCGGGCCATC CGGGTGTACA TGGGGGAAAG TGATGTGTAC
    AGCGTGCAGC ACATTGTCTG GCACGTGGAG GAAGGAGGGC CTGCCCATGA AGCGGGTCTC
    TGTGCCGGGG ACCTCATTAC ACACATTAAC GGGGAGCCGG TGCACGGCCT GGTGCACACT
    GAAGTTGTGG AGCTGATTTT AAAGAGCGGT AACAAGGTGT TAGTAACCAC CACCCCCTTT
    GAGAACACCT CGATCCGAAC CGGCCCAGCC AGGAGAAGCA GTTATCGATC CAAAATGGCG
    CGGAGGAACA AGCGGATCGC TGGGAGAGAG GGGCAGGAGA GTAAGAAGCG GAGCTCTCTA
    TTCCGTAAGA TCACCAAGCA ATCCAATCTT CTGCACACCA GCCGTTCCCT CTCCTCTCTG
    AGCCGCTCCC TCTCCTCAAG TGACAGTCTT CCAGGATCAC CCACTCACAG CCTCTCGGCC
    CGTTCCCCGA CCCATAGCTA CCGCTCCATG GACTCTGCTT ACCTTGGTGC TTCTTCGCAG
    GGCAGTTCCC CCTCTTCCAG TACCCCTAAT TCCCCTGCAC CACCACCTCC TGCCTCAGCC
    TCTTCCTGTG CGTCAGCCTC CTCCTCCTCC ACCACTCCTG CTCCCCCCTC CCCACACATC
    CGACCCAGCA CCCTGCATGG CTTATCTCCC AAACTGCACC GCCAGTATCG CTCAGCGCGA
    TGTAAATCTG CGGGTAATAT GCCCCTCTCT CCCCTTGCAC ACACCCCTTC CCCTACTGCA
    GCTGCCTCAC CCCCTCTCCT ACCAAGTCAC AGCATTGGCA GCAGCCTGAC CACTCAGAGC
    TTTCCCCCAA AACTGCATCC ATCTCCACCA GTGTCACGCC CTCGCCCAAA GAGCGCAGAG
    CCTCCACGGT CCCCACTTCT TAAGAGAGTG CAGTCTGCAG AGAAACTGGG TGGGGGAGGA
    GGTGGTGGGT CACCCAGAAA ACAGGAGCCG GGAGGGGGAG GAACAATAGA GGGAGATTTA
    GCAGAAGCCA TTGGTGTGAG GAGCCAGCCA GTAAGGAGAG TGGGTAGGCA GGAGTCTCCT
    CGCAGTCTTT GTGGAGGGGA AAATATGATA GGAGAGAACT TTGAGAGGCT GGAAGGAGAA
    GGAGCGATAG GACTATCGCC GCTAGGAGAC AGTCTGAAAT GTAGTGAGGG TAAAGTCCAA
    GCAGCCGTTA AGGTTGGAGT AGGATCAAGG ACTACTCCCC CATTTTCTCC CCAAGAACCT
    CCAGCCGCAT CAACTGCAGT CCTTCCCCCC CAAAGTGACC CTTCCCTTGC TATGGTAGGA
    AGGTGCCAAG TGAAAACCCT AAGTCCTGTA CAAGAACATG AAGGTAGAAG GGGGGAAGGA
    GAGGACGGGG GAGCAGAGGG AGAGGGAAGA AAGGGAGATT ATGAGGAGGA ACAAGGAAGA
    ACAAGAGAAC AATGGTACTT AGAGGTGGTA GAAGAGTTGA CTACAGTAGG GAAAAGCAGC
    ATGCATGCTG GGAGTAAAGC GACTCCCTCT AACCTCACAG CCGACTCTAG TATCTACCAT
    TCCCACTCAA ATAAGAGTAA AGTCGCTGTG GTTGACTCTA TGAAGAGCAC ACCCTCTCAC
    CCTACAGGTA CAACTCTGGA GCAGTCTCTT TCTTTGAAAG GGATTAGATC TGATGCCGAC
    TCTAAAAAGG GGCAAGATCC TAAAAACTGC CAGATTACTC CTCTTCTTCC CGTAAGGGAG
    GGCCTAAATG TTGAACCAAT TCAAAATGGT TCTACTCACC CTCCTGAACT TTCCCACGTG
    GCCAAGGAGC AAAGTATCGA CCATCTAAAA GGTCCATCAT CTTCTGGCCA ACAAATGAAA
    AGCACTAGCA CTTTCCTACC TACTCAGCTT CACCCAACCA CTGGTAGAAA GCTGGAGGCT
    GATAATGTAA GTAGGCATTT ACTCTGTACA GAGGGTACAA AGTATGGTCT GATTAAACCA
    ACAGATTCTC TGTCTATTAG TATTAAAGGG GAGAAGGCCA GTTTAGCAAA AAGCCCCCAG
    GATGATCCAG TAAAGGCCGC TGATCATACT ACTGGGGTGC AAGGACCTGC TCCTGCAAAA
    GGACCAGCAA TTTCACCTGT AAATATTAAA ATCGGCAGAG AAGGATCTGA ACAGACAAGG
    GTGACCACTA CTGACCCAAC TATTAATAGG CTGAGCGAAA CACAAACTAA TCAGAGACCT
    GCAGCTGCCA CTGGTCTTGC TAGCAATATG GTACTTAGGG AGATGGAGAA GAAGGAAGAG
    TTCAGTTCAG CCCAGAATAT AAAGAAACCA AGTGGAGCAG AATTAGAACA GAAGCGGGTG
    GCCATTGTAG TCCCGATTGT AACTAGACCC AGTGATGTAA AGGCTGACAC AACAAGGGTA
    GTATCTGCTC CCCTGACTGT TAATAGACCT GCCGCAGGAG AGAGTGACCA AGCAAAAGTG
    ACCACTGTTA CCCCAACTAT TGATAAAGTC AGTGGCCCAG AAAGTGCCTC TGCCAAGGTG
    ACCGCCGTTG CCTCAATGAG TAGCGCTAAC AAAGCTGTTA ATGAACCACC AAGCCTGTTC
    CCCGATAATA AAGTAGCCGA CTCCAATCAA ATAAGGTTCT CGTCACAGAA TAACAAGGGC
    GCGAGAGAAT CAGAAAGTGA CCAGATAAGG GCAACGGGCA ATATTCCCGT TATTAACCAG
    ACCGCTCAAA CAACGGTTCC TTCCCCAACA CCCACAAATG GCCAAGGACC CCCTATAATT
    ATCCGAGTTA GTGGGGTTCC ATGTGATGCC CAGGGCGGGC TGTGTCCTGA GAAACCTTCC
    AGCCCCCAAA GAGCGCCCAC CACTACCAGC CAACCCCCTT CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55398
    Clone ID Related Accession (Same CDS sequence) XM_031899447.1
    Accession Version XM_031899447.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product microtubule-associated serine/threonine-protein kinase 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGATGATT CTGGCATTAT CAGACGCAGG AGGCTGCAGG ATCTGCCCCT GCCGAGGAAG 
    AGCAGCGGTT GCAGAAGCAG CAACAGAAAG AGTTTAATCC TCACAAGCAG CAGTTCCCCC
    ACTCTCCCCC GGCCCCACTC CCCCCTGCCG GGGCATATAG GTGGGAGCCC TATGGACAGT
    CCCAGGAACT TCTCCCCCAG CGCCGCTGCT CACTTCTCCT TTGCATCATC TCGCAGAACC
    GATGGCCGTC GCTGGTCCTT GGCCTCCCTC CCGTCGTCCG GCTATGGGAC AAATACCCCG
    AGTTCCACGG TGTCGTCCTC CTCCTCCTCT CAGGAACGCC TACACCAGCT CCCCTTCCAG
    CCCACACTGG ATGAGCTCCA CTTCCTTTCC AAACACTTTG GCAGCACAGA AAGTATCCCC
    GATGATGATG GGGGGCGCAG GTCACCTGCG GTCAGACCAC GATCCCGCAG CTTAAGTCCT
    GGCCGATCTC CCTCCTCTTA TGACAATGAA ATTGTGATGA TGAACCATGT TTATAAAGAG
    AGGTTTCCCA AGGCCACAGC GCAGATGGAG GATAGGCTAC GGATGTTCAT CTCCTCCTTC
    TCCCCCGAGA ATGTCCTACC ACTGGCGGAC GGACTCCTCA ACTTTATCCA CCATCAGATC
    GTGGAGCTGG CGAGGGACTG CCTGAACAGG AGCACCGAGG GCATGATCAC AACCGCCCAC
    TTTTACCAAC TACAGGAGAA TCTGGAGAAG CTGCTGAATG ATGCAGTGGA ACGTTCTTCA
    GAGGGATCAG AAGTTGCCTT CATTGCTCAA CTGATGAAAA ATCTTTTAAT CATCATCTCT
    CGACCTGCTC GTCTGCTGGA GTGTCTGGAA TTTAACCCTG AAGAGTTCTA TCACATGCTG
    GAGGTAGCCG AGGACCAGGT GAAAGAAGGA AACTTGAAAA AAGACATACC CCAATACATC
    CTGAGACAGT TGGGATTAAC CAGGGATTCC TTCCCAGAGG TAGCCGCTCC CGAGGAACCA
    GTGACCCTGG AAGCACAAAC CTCAGAAGCA GAGGACAACG TAGAGGCAAA GAGTGGGGCG
    GCCCATAAAC CCATCAGACC ACCCGAGGAA AGTGACTTTG AAAATATAAA GCTCATCAGT
    AACGGAGCCT ATGGAGCGGT GTATTTGGTG CGTCACCGGG AGACGCGGCA ACGCTTTGCC
    ATGAAGAAGA TCAACAAGCA GAACTTGCGT CTGAGGAAAC AAGTCCACCA GGCCTTTGTA
    GAACGGGACA TTCTGACCTT TGCTCAGAAC CCCTTTGTGG TCAGCATGTT CTGCTCCTTC
    CAGACCCGTA GGCACCTCTG TATGGTGATG GAGTATGTGG AAGGGGGTGA CTGTGCCACC
    CTACTGAAGC ACATTGGTGC TCTCCCATTG GAAATGGCGC GGTTATATTT CGCAGAGACG
    GTTCTAGCTC TGGAATACCT ACATAATTAT GGGATTGTGC ACCGAGATCT GAAACCGGAC
    AATCTCCTAA TCACTTCGAT GGGTCACATA AAGCTTACAG ATTTCGGCCT GTCCAAGATG
    GGGCTTATGA ACCTCACCAC TAATCTCTAT GAAGGACACA TCGAGAGCGA AGCAAGGGAA
    TTCCTGGACA AGCAGGTCTG TGGAACCCCT GAATACATCG CACCGGAGGT TATCCTTAGA
    CAAGGTTACG GGAAGCCAGT GGACTGGTGG GCGATGGGCA TAATTCTGTA TGAGTTCCTG
    GTTGGTTGCG TGCCATTTTT TGGAGACACC CCAGAGGAGC TGTTTGGTCA GGTGATCAGT
    GATGACATTG TGTGGCCAGA AGCAGAGGAA GCTCTACCCG CCGATGCCCA AAATCTCATC
    TCCTGCCTCC TGCAGACAGA TCCCTTACAA AGGCTTGGAA CAGGGGGAGC CATTGAGGTG
    AAAATTCACA GATTTTTCAC TGATTTGGAC TGGAATGGAT TGCTCAGGCA GAAAGCTGAA
    TTCATCCCTC ACTTGGAGGC AGAAGATGAT ACCAGTTACT TTGACACTCG ATCGGATCGG
    TACCAGCACA TTAATTCCTA CGATGAGGAT GATACCAATG ATGAAGACTC GGTGGAGATC
    AGGCAGTTCT CATCCTGCAC CCCAAGATTT AGTAAGGTGT ATAGTAGTAT GGAGCAGCTC
    TCCCAGCTGG AGCAGAAACC CCCCACTCCT CCTAGAGACA CCAACAAACA AGAAAGGTCT
    GAGAAGACGA CCAAGAGAGA GACGTTGGGG GGCGTCACCC TTCGAGACCG CTGCTGGAGG
    ACCGTGTCTC CTGAAATGAA ACAATTCTCC GATGCGGCAG ACGCAGAATC CAGCCCCCCC
    TTGGCTGCCC GGCGGCGCTT TTCTGCCTTA GTGGACCCGA CCCGCTATAC GTCTCCCCTG
    GAGTATGAGG TGGAGTACAG AGTGAGGGGA AGCAGCGGGC CGCTGCCCGC CCAAAGCAAG
    ATCCCTCTGG GAGAGCTGGA GCCAGTAGAC AAGTCTGTCG ACTCTCAGCC CCACCAGGAG
    GCGGATTCCT CTCCTCGCGC CACAAACGAG ATTGTCCTGC GCAGAGCCAG GCACCAACAC
    CTGTCCGGGG ACACCGAGAG GAGGAGAACC GGCGGGAAAG TCATCAAGTC GGCATCGACC
    ACCGCCCTGT CTGTCATGAT CCCTACAGTT GAACAGCACA GCAGCTCTCC CCTGGTCAGC
    CCCATGTCCC CCCGCTCTCT GTCGTCCAAT CCCTCCTCCC GGGATTCCTC TCCGAGCCGC
    GACTACACGC CGGCAGTGAG CAGCCTCCGA TCCCCCATTT GCATCCAACG CTCCGGGAAA
    AAATACGGTT TCACCTTGCG GGCCATCCGG GTGTACATGG GGGAAAGTGA TGTGTACAGC
    GTGCAGCACA TTGTCTGGCA CGTGGAGGAA GGAGGGCCTG CCCATGAAGC GGGTCTCTGT
    GCCGGGGACC TCATTACACA CATTAACGGG GAGCCGGTGC ACGGCCTGGT GCACACTGAA
    GTTGTGGAGC TGATTTTAAA GAGCGGTAAC AAGGTGTTAG TAACCACCAC CCCCTTTGAG
    AACACCTCGA TCCGAACCGG CCCAGCCAGG AGAAGCAGTT ATCGATCCAA AATGGCGCGG
    AGGAACAAGC GGATCGCTGG GAGAGAGGGG CAGGAGAGTA AGAAGCGGAG CTCTCTATTC
    CGTAAGATCA CCAAGCAATC CAATCTTCTG CACACCAGCC GTTCCCTCTC CTCTCTGAGC
    CGCTCCCTCT CCTCAAGTGA CAGTCTTCCA GGATCACCCA CTCACAGCCT CTCGGCCCGT
    TCCCCGACCC ATAGCTACCG CTCCATGGAC TCTGCTTACC TTGGTGCTTC TTCGCAGGGC
    AGTTCCCCCT CTTCCAGTAC CCCTAATTCC CCTGCACCAC CACCTCCTGC CTCAGCCTCT
    TCCTGTGCGT CAGCCTCCTC CTCCTCCACC ACTCCTGCTC CCCCCTCCCC ACACATCCGA
    CCCAGCACCC TGCATGGCTT ATCTCCCAAA CTGCACCGCC AGTATCGCTC AGCGCGATGT
    AAATCTGCGG GTAATATGCC CCTCTCTCCC CTTGCACACA CCCCTTCCCC TACTGCAGCT
    GCCTCACCCC CTCTCCTACC AAGTCACAGC ATTGGCAGCA GCCTGACCAC TCAGAGCTTT
    CCCCCAAAAC TGCATCCATC TCCACCAGTG TCACGCCCTC GCCCAAAGAG CGCAGAGCCT
    CCACGGTCCC CACTTCTTAA GAGAGTGCAG TCTGCAGAGA AACTGGGTGG GGGAGGAGGT
    GGTGGGTCAC CCAGAAAACA GGAGCCGGGA GGGGGAGGAA CAATAGAGGG AGATTTAGCA
    GAAGCCATTG GTGTGAGGAG CCAGCCAGTA AGGAGAGTGG GTAGGCAGGA GTCTCCTCGC
    AGTCTTTGTG GAGGGGAAAA TATGATAGGA GAGAACTTTG AGAGGCTGGA AGGAGAAGGA
    GCGATAGGAC TATCGCCGCT AGGAGACAGT CTGAAATGTA GTGAGGGTAA AGTCCAAGCA
    GCCGTTAAGG TTGGAGTAGG ATCAAGGACT ACTCCCCCAT TTTCTCCCCA AGAACCTCCA
    GCCGCATCAA CTGCAGTCCT TCCCCCCCAA AGTGACCCTT CCCTTGCTAT GGTAGGAAGG
    TGCCAAGTGA AAACCCTAAG TCCTGTACAA GAACATGAAG GTAGAAGGGG GGAAGGAGAG
    GACGGGGGAG CAGAGGGAGA GGGAAGAAAG GGAGATTATG AGGAGGAACA AGGAAGAACA
    AGAGAACAAT GGTACTTAGA GGTGGTAGAA GAGTTGACTA CAGTAGGGAA AAGCAGCATG
    CATGCTGGGA GTAAAGCGAC TCCCTCTAAC CTCACAGCCG ACTCTAGTAT CTACCATTCC
    CACTCAAATA AGAGTAAAGT CGCTGTGGTT GACTCTATGA AGAGCACACC CTCTCACCCT
    ACAGGTACAA CTCTGGAGCA GTCTCTTTCT TTGAAAGGGA TTAGATCTGA TGCCGACTCT
    AAAAAGGGGC AAGATCCTAA AAACTGCCAG ATTACTCCTC TTCTTCCCGT AAGGGAGGGC
    CTAAATGTTG AACCAATTCA AAATGGTTCT ACTCACCCTC CTGAACTTTC CCACGTGGCC
    AAGGAGCAAA GTATCGACCA TCTAAAAGGT CCATCATCTT CTGGCCAACA AATGAAAAGC
    ACTAGCACTT TCCTACCTAC TCAGCTTCAC CCAACCACTG GTAGAAAGCT GGAGGCTGAT
    AATGTAAGTA GGCATTTACT CTGTACAGAG GGTACAAAGT ATGGTCTGAT TAAACCAACA
    GATTCTCTGT CTATTAGTAT TAAAGGGGAG AAGGCCAGTT TAGCAAAAAG CCCCCAGGAT
    GATCCAGTAA AGGCCGCTGA TCATACTACT GGGGTGCAAG GACCTGCTCC TGCAAAAGGA
    CCAGCAATTT CACCTGTAAA TATTAAAATC GGCAGAGAAG GATCTGAACA GACAAGGGTG
    ACCACTACTG ACCCAACTAT TAATAGGCTG AGCGAAACAC AAACTAATCA GAGACCTGCA
    GCTGCCACTG GTCTTGCTAG CAATATGGTA CTTAGGGAGA TGGAGAAGAA GGAAGAGTTC
    AGTTCAGCCC AGAATATAAA GAAACCAAGT GGAGCAGAAT TAGAACAGAA GCGGGTGGCC
    ATTGTAGTCC CGATTGTAAC TAGACCCAGT GATGTAAAGG CTGACACAAC AAGGGTAGTA
    TCTGCTCCCC TGACTGTTAA TAGACCTGCC GCAGGAGAGA GTGACCAAGC AAAAGTGACC
    ACTGTTACCC CAACTATTGA TAAAGTCAGT GGCCCAGAAA GTGCCTCTGC CAAGGTGACC
    GCCGTTGCCT CAATGAGTAG CGCTAACAAA GCTGTTAATG AACCACCAAG CCTGTTCCCC
    GATAATAAAG TAGCCGACTC CAATCAAATA AGGTTCTCGT CACAGAATAA CAAGGGCGCG
    AGAGAATCAG AAAGTGACCA GATAAGGGCA ACGGGCAATA TTCCCGTTAT TAACCAGACC
    GCTCAAACAA CGGTTCCTTC CCCAACACCC ACAAATGGCC AAGGACCCCC TATAATTATC
    CGAGTTAGTG GGGTTCCATG TGATGCCCAG GGCGGGCTGT GTCCTGAGAA ACCTTCCAGC
    CCCCAAAGAG CGCCCACCAC TACCAGCCAA CCCCCTTCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755307.1
    CDS67..5748
    Translation

    Target ORF information:

    RefSeq Version XM_031899447.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis microtubule associated serine/threonine kinase 1 (mast1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899447.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGATGATT CTGGCATTAT CAGACGCAGG AGGCTGCAGG ATCTGCCCCT GCCGAGGAAG 
    AGCAGCGGTT GCAGAAGCAG CAACAGAAAG AGTTTAATCC TCACAAGCAG CAGTTCCCCC
    ACTCTCCCCC GGCCCCACTC CCCCCTGCCG GGGCATATAG GTGGGAGCCC TATGGACAGT
    CCCAGGAACT TCTCCCCCAG CGCCGCTGCT CACTTCTCCT TTGCATCATC TCGCAGAACC
    GATGGCCGTC GCTGGTCCTT GGCCTCCCTC CCGTCGTCCG GCTATGGGAC AAATACCCCG
    AGTTCCACGG TGTCGTCCTC CTCCTCCTCT CAGGAACGCC TACACCAGCT CCCCTTCCAG
    CCCACACTGG ATGAGCTCCA CTTCCTTTCC AAACACTTTG GCAGCACAGA AAGTATCCCC
    GATGATGATG GGGGGCGCAG GTCACCTGCG GTCAGACCAC GATCCCGCAG CTTAAGTCCT
    GGCCGATCTC CCTCCTCTTA TGACAATGAA ATTGTGATGA TGAACCATGT TTATAAAGAG
    AGGTTTCCCA AGGCCACAGC GCAGATGGAG GATAGGCTAC GGATGTTCAT CTCCTCCTTC
    TCCCCCGAGA ATGTCCTACC ACTGGCGGAC GGACTCCTCA ACTTTATCCA CCATCAGATC
    GTGGAGCTGG CGAGGGACTG CCTGAACAGG AGCACCGAGG GCATGATCAC AACCGCCCAC
    TTTTACCAAC TACAGGAGAA TCTGGAGAAG CTGCTGAATG ATGCAGTGGA ACGTTCTTCA
    GAGGGATCAG AAGTTGCCTT CATTGCTCAA CTGATGAAAA ATCTTTTAAT CATCATCTCT
    CGACCTGCTC GTCTGCTGGA GTGTCTGGAA TTTAACCCTG AAGAGTTCTA TCACATGCTG
    GAGGTAGCCG AGGACCAGGT GAAAGAAGGA AACTTGAAAA AAGACATACC CCAATACATC
    CTGAGACAGT TGGGATTAAC CAGGGATTCC TTCCCAGAGG TAGCCGCTCC CGAGGAACCA
    GTGACCCTGG AAGCACAAAC CTCAGAAGCA GAGGACAACG TAGAGGCAAA GAGTGGGGCG
    GCCCATAAAC CCATCAGACC ACCCGAGGAA AGTGACTTTG AAAATATAAA GCTCATCAGT
    AACGGAGCCT ATGGAGCGGT GTATTTGGTG CGTCACCGGG AGACGCGGCA ACGCTTTGCC
    ATGAAGAAGA TCAACAAGCA GAACTTGCGT CTGAGGAAAC AAGTCCACCA GGCCTTTGTA
    GAACGGGACA TTCTGACCTT TGCTCAGAAC CCCTTTGTGG TCAGCATGTT CTGCTCCTTC
    CAGACCCGTA GGCACCTCTG TATGGTGATG GAGTATGTGG AAGGGGGTGA CTGTGCCACC
    CTACTGAAGC ACATTGGTGC TCTCCCATTG GAAATGGCGC GGTTATATTT CGCAGAGACG
    GTTCTAGCTC TGGAATACCT ACATAATTAT GGGATTGTGC ACCGAGATCT GAAACCGGAC
    AATCTCCTAA TCACTTCGAT GGGTCACATA AAGCTTACAG ATTTCGGCCT GTCCAAGATG
    GGGCTTATGA ACCTCACCAC TAATCTCTAT GAAGGACACA TCGAGAGCGA AGCAAGGGAA
    TTCCTGGACA AGCAGGTCTG TGGAACCCCT GAATACATCG CACCGGAGGT TATCCTTAGA
    CAAGGTTACG GGAAGCCAGT GGACTGGTGG GCGATGGGCA TAATTCTGTA TGAGTTCCTG
    GTTGGTTGCG TGCCATTTTT TGGAGACACC CCAGAGGAGC TGTTTGGTCA GGTGATCAGT
    GATGACATTG TGTGGCCAGA AGCAGAGGAA GCTCTACCCG CCGATGCCCA AAATCTCATC
    TCCTGCCTCC TGCAGACAGA TCCCTTACAA AGGCTTGGAA CAGGGGGAGC CATTGAGGTG
    AAAATTCACA GATTTTTCAC TGATTTGGAC TGGAATGGAT TGCTCAGGCA GAAAGCTGAA
    TTCATCCCTC ACTTGGAGGC AGAAGATGAT ACCAGTTACT TTGACACTCG ATCGGATCGG
    TACCAGCACA TTAATTCCTA CGATGAGGAT GATACCAATG ATGAAGACTC GGTGGAGATC
    AGGCAGTTCT CATCCTGCAC CCCAAGATTT AGTAAGGTGT ATAGTAGTAT GGAGCAGCTC
    TCCCAGCTGG AGCAGAAACC CCCCACTCCT CCTAGAGACA CCAACAAACA AGAAAGGTCT
    GAGAAGACGA CCAAGAGAGA GACGTTGGGG GGCGTCACCC TTCGAGACCG CTGCTGGAGG
    ACCGTGTCTC CTGAAATGAA ACAATTCTCC GATGCGGCAG ACGCAGAATC CAGCCCCCCC
    TTGGCTGCCC GGCGGCGCTT TTCTGCCTTA GTGGACCCGA CCCGCTATAC GTCTCCCCTG
    GAGTATGAGG TGGAGTACAG AGTGAGGGGA AGCAGCGGGC CGCTGCCCGC CCAAAGCAAG
    ATCCCTCTGG GAGAGCTGGA GCCAGTAGAC AAGTCTGTCG ACTCTCAGCC CCACCAGGAG
    GCGGATTCCT CTCCTCGCGC CACAAACGAG ATTGTCCTGC GCAGAGCCAG GCACCAACAC
    CTGTCCGGGG ACACCGAGAG GAGGAGAACC GGCGGGAAAG TCATCAAGTC GGCATCGACC
    ACCGCCCTGT CTGTCATGAT CCCTACAGTT GAACAGCACA GCAGCTCTCC CCTGGTCAGC
    CCCATGTCCC CCCGCTCTCT GTCGTCCAAT CCCTCCTCCC GGGATTCCTC TCCGAGCCGC
    GACTACACGC CGGCAGTGAG CAGCCTCCGA TCCCCCATTT GCATCCAACG CTCCGGGAAA
    AAATACGGTT TCACCTTGCG GGCCATCCGG GTGTACATGG GGGAAAGTGA TGTGTACAGC
    GTGCAGCACA TTGTCTGGCA CGTGGAGGAA GGAGGGCCTG CCCATGAAGC GGGTCTCTGT
    GCCGGGGACC TCATTACACA CATTAACGGG GAGCCGGTGC ACGGCCTGGT GCACACTGAA
    GTTGTGGAGC TGATTTTAAA GAGCGGTAAC AAGGTGTTAG TAACCACCAC CCCCTTTGAG
    AACACCTCGA TCCGAACCGG CCCAGCCAGG AGAAGCAGTT ATCGATCCAA AATGGCGCGG
    AGGAACAAGC GGATCGCTGG GAGAGAGGGG CAGGAGAGTA AGAAGCGGAG CTCTCTATTC
    CGTAAGATCA CCAAGCAATC CAATCTTCTG CACACCAGCC GTTCCCTCTC CTCTCTGAGC
    CGCTCCCTCT CCTCAAGTGA CAGTCTTCCA GGATCACCCA CTCACAGCCT CTCGGCCCGT
    TCCCCGACCC ATAGCTACCG CTCCATGGAC TCTGCTTACC TTGGTGCTTC TTCGCAGGGC
    AGTTCCCCCT CTTCCAGTAC CCCTAATTCC CCTGCACCAC CACCTCCTGC CTCAGCCTCT
    TCCTGTGCGT CAGCCTCCTC CTCCTCCACC ACTCCTGCTC CCCCCTCCCC ACACATCCGA
    CCCAGCACCC TGCATGGCTT ATCTCCCAAA CTGCACCGCC AGTATCGCTC AGCGCGATGT
    AAATCTGCGG GTAATATGCC CCTCTCTCCC CTTGCACACA CCCCTTCCCC TACTGCAGCT
    GCCTCACCCC CTCTCCTACC AAGTCACAGC ATTGGCAGCA GCCTGACCAC TCAGAGCTTT
    CCCCCAAAAC TGCATCCATC TCCACCAGTG TCACGCCCTC GCCCAAAGAG CGCAGAGCCT
    CCACGGTCCC CACTTCTTAA GAGAGTGCAG TCTGCAGAGA AACTGGGTGG GGGAGGAGGT
    GGTGGGTCAC CCAGAAAACA GGAGCCGGGA GGGGGAGGAA CAATAGAGGG AGATTTAGCA
    GAAGCCATTG GTGTGAGGAG CCAGCCAGTA AGGAGAGTGG GTAGGCAGGA GTCTCCTCGC
    AGTCTTTGTG GAGGGGAAAA TATGATAGGA GAGAACTTTG AGAGGCTGGA AGGAGAAGGA
    GCGATAGGAC TATCGCCGCT AGGAGACAGT CTGAAATGTA GTGAGGGTAA AGTCCAAGCA
    GCCGTTAAGG TTGGAGTAGG ATCAAGGACT ACTCCCCCAT TTTCTCCCCA AGAACCTCCA
    GCCGCATCAA CTGCAGTCCT TCCCCCCCAA AGTGACCCTT CCCTTGCTAT GGTAGGAAGG
    TGCCAAGTGA AAACCCTAAG TCCTGTACAA GAACATGAAG GTAGAAGGGG GGAAGGAGAG
    GACGGGGGAG CAGAGGGAGA GGGAAGAAAG GGAGATTATG AGGAGGAACA AGGAAGAACA
    AGAGAACAAT GGTACTTAGA GGTGGTAGAA GAGTTGACTA CAGTAGGGAA AAGCAGCATG
    CATGCTGGGA GTAAAGCGAC TCCCTCTAAC CTCACAGCCG ACTCTAGTAT CTACCATTCC
    CACTCAAATA AGAGTAAAGT CGCTGTGGTT GACTCTATGA AGAGCACACC CTCTCACCCT
    ACAGGTACAA CTCTGGAGCA GTCTCTTTCT TTGAAAGGGA TTAGATCTGA TGCCGACTCT
    AAAAAGGGGC AAGATCCTAA AAACTGCCAG ATTACTCCTC TTCTTCCCGT AAGGGAGGGC
    CTAAATGTTG AACCAATTCA AAATGGTTCT ACTCACCCTC CTGAACTTTC CCACGTGGCC
    AAGGAGCAAA GTATCGACCA TCTAAAAGGT CCATCATCTT CTGGCCAACA AATGAAAAGC
    ACTAGCACTT TCCTACCTAC TCAGCTTCAC CCAACCACTG GTAGAAAGCT GGAGGCTGAT
    AATGTAAGTA GGCATTTACT CTGTACAGAG GGTACAAAGT ATGGTCTGAT TAAACCAACA
    GATTCTCTGT CTATTAGTAT TAAAGGGGAG AAGGCCAGTT TAGCAAAAAG CCCCCAGGAT
    GATCCAGTAA AGGCCGCTGA TCATACTACT GGGGTGCAAG GACCTGCTCC TGCAAAAGGA
    CCAGCAATTT CACCTGTAAA TATTAAAATC GGCAGAGAAG GATCTGAACA GACAAGGGTG
    ACCACTACTG ACCCAACTAT TAATAGGCTG AGCGAAACAC AAACTAATCA GAGACCTGCA
    GCTGCCACTG GTCTTGCTAG CAATATGGTA CTTAGGGAGA TGGAGAAGAA GGAAGAGTTC
    AGTTCAGCCC AGAATATAAA GAAACCAAGT GGAGCAGAAT TAGAACAGAA GCGGGTGGCC
    ATTGTAGTCC CGATTGTAAC TAGACCCAGT GATGTAAAGG CTGACACAAC AAGGGTAGTA
    TCTGCTCCCC TGACTGTTAA TAGACCTGCC GCAGGAGAGA GTGACCAAGC AAAAGTGACC
    ACTGTTACCC CAACTATTGA TAAAGTCAGT GGCCCAGAAA GTGCCTCTGC CAAGGTGACC
    GCCGTTGCCT CAATGAGTAG CGCTAACAAA GCTGTTAATG AACCACCAAG CCTGTTCCCC
    GATAATAAAG TAGCCGACTC CAATCAAATA AGGTTCTCGT CACAGAATAA CAAGGGCGCG
    AGAGAATCAG AAAGTGACCA GATAAGGGCA ACGGGCAATA TTCCCGTTAT TAACCAGACC
    GCTCAAACAA CGGTTCCTTC CCCAACACCC ACAAATGGCC AAGGACCCCC TATAATTATC
    CGAGTTAGTG GGGTTCCATG TGATGCCCAG GGCGGGCTGT GTCCTGAGAA ACCTTCCAGC
    CCCCAAAGAG CGCCCACCAC TACCAGCCAA CCCCCTTCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55399
    Clone ID Related Accession (Same CDS sequence) XM_031899448.1
    Accession Version XM_031899448.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5637bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product microtubule-associated serine/threonine-protein kinase 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGTTCCGCC GCACCAAGAG TTGCAGAAGC AGCAACAGAA AGAGTTTAAT CCTCACAAGC 
    AGCAGTTCCC CCACTCTCCC CCGGCCCCAC TCCCCCCTGC CGGGGCATAT AGGTGGGAGC
    CCTATGGACA GTCCCAGGAA CTTCTCCCCC AGCGCCGCTG CTCACTTCTC CTTTGCATCA
    TCTCGCAGCA GAACCGATGG CCGTCGCTGG TCCTTGGCCT CCCTCCCGTC GTCCGGCTAT
    GGGACAAATA CCCCGAGTTC CACGGTGTCG TCCTCCTCCT CCTCTCAGGA ACGCCTACAC
    CAGCTCCCCT TCCAGCCCAC ACTGGATGAG CTCCACTTCC TTTCCAAACA CTTTGGCAGC
    ACAGAAAGTA TCCCCGATGA TGATGGGGGG CGCAGGTCAC CTGCGGTCAG ACCACGATCC
    CGCAGCTTAA GTCCTGGCCG ATCTCCCTCC TCTTATGACA ATGAAATTGT GATGATGAAC
    CATGTTTATA AAGAGAGGTT TCCCAAGGCC ACAGCGCAGA TGGAGGATAG GCTACGGATG
    TTCATCTCCT CCTTCTCCCC CGAGAATGTC CTACCACTGG CGGACGGACT CCTCAACTTT
    ATCCACCATC AGATCGTGGA GCTGGCGAGG GACTGCCTGA ACAGGAGCAC CGAGGGCATG
    ATCACAACCG CCCACTTTTA CCAACTACAG GAGAATCTGG AGAAGCTGCT GAATGATGCA
    GTGGAACGTT CTTCAGAGGG ATCAGAAGTT GCCTTCATTG CTCAACTGAT GAAAAATCTT
    TTAATCATCA TCTCTCGACC TGCTCGTCTG CTGGAGTGTC TGGAATTTAA CCCTGAAGAG
    TTCTATCACA TGCTGGAGGT AGCCGAGGAC CAGGTGAAAG AAGGAAACTT GAAAAAAGAC
    ATACCCCAAT ACATCCTGAG ACAGTTGGGA TTAACCAGGG ATTCCTTCCC AGAGGTAGCC
    GCTCCCGAGG AACCAGTGAC CCTGGAAGCA CAAACCTCAG AAGCAGAGGA CAACGTAGAG
    GCAAAGAGTG GGGCGGCCCA TAAACCCATC AGACCACCCG AGGAAAGTGA CTTTGAAAAT
    ATAAAGCTCA TCAGTAACGG AGCCTATGGA GCGGTGTATT TGGTGCGTCA CCGGGAGACG
    CGGCAACGCT TTGCCATGAA GAAGATCAAC AAGCAGAACT TGCGTCTGAG GAAACAAGTC
    CACCAGGCCT TTGTAGAACG GGACATTCTG ACCTTTGCTC AGAACCCCTT TGTGGTCAGC
    ATGTTCTGCT CCTTCCAGAC CCGTAGGCAC CTCTGTATGG TGATGGAGTA TGTGGAAGGG
    GGTGACTGTG CCACCCTACT GAAGCACATT GGTGCTCTCC CATTGGAAAT GGCGCGGTTA
    TATTTCGCAG AGACGGTTCT AGCTCTGGAA TACCTACATA ATTATGGGAT TGTGCACCGA
    GATCTGAAAC CGGACAATCT CCTAATCACT TCGATGGGTC ACATAAAGCT TACAGATTTC
    GGCCTGTCCA AGATGGGGCT TATGAACCTC ACCACTAATC TCTATGAAGG ACACATCGAG
    AGCGAAGCAA GGGAATTCCT GGACAAGCAG GTCTGTGGAA CCCCTGAATA CATCGCACCG
    GAGGTTATCC TTAGACAAGG TTACGGGAAG CCAGTGGACT GGTGGGCGAT GGGCATAATT
    CTGTATGAGT TCCTGGTTGG TTGCGTGCCA TTTTTTGGAG ACACCCCAGA GGAGCTGTTT
    GGTCAGGTGA TCAGTGATGA CATTGTGTGG CCAGAAGCAG AGGAAGCTCT ACCCGCCGAT
    GCCCAAAATC TCATCTCCTG CCTCCTGCAG ACAGATCCCT TACAAAGGCT TGGAACAGGG
    GGAGCCATTG AGGTGAAAAT TCACAGATTT TTCACTGATT TGGACTGGAA TGGATTGCTC
    AGGCAGAAAG CTGAATTCAT CCCTCACTTG GAGGCAGAAG ATGATACCAG TTACTTTGAC
    ACTCGATCGG ATCGGTACCA GCACATTAAT TCCTACGATG AGGATGATAC CAATGATGAA
    GACTCGGTGG AGATCAGGCA GTTCTCATCC TGCACCCCAA GATTTAGTAA GGTGTATAGT
    AGTATGGAGC AGCTCTCCCA GCTGGAGCAG AAACCCCCCA CTCCTCCTAG AGACACCAAC
    AAACAAGAAA GGTCTGAGAA GACGACCAAG AGAGAGACGT TGGGGGGCGT CACCCTTCGA
    GACCGCTGCT GGAGGACCGT GTCTCCTGAA ATGAAACAAT TCTCCGATGC GGCAGACGCA
    GAATCCAGCC CCCCCTTGGC TGCCCGGCGG CGCTTTTCTG CCTTAGTGGA CCCGACCCGC
    TATACGTCTC CCCTGGAGTA TGAGGTGGAG TACAGAGTGA GGGGAAGCAG CGGGCCGCTG
    CCCGCCCAAA GCAAGATCCC TCTGGGAGAG CTGGAGCCAG TAGACAAGTC TGTCGACTCT
    CAGCCCCACC AGGAGGCGGA TTCCTCTCCT CGCGCCACAA ACGAGATTGT CCTGCGCAGA
    GCCAGGCACC AACACCTGTC CGGGGACACC GAGAGGAGGA GAACCGGCGG GAAAGTCATC
    AAGTCGGCAT CGACCACCGC CCTGTCTGTC ATGATCCCTA CAGTTGAACA GCACAGCAGC
    TCTCCCCTGG TCAGCCCCAT GTCCCCCCGC TCTCTGTCGT CCAATCCCTC CTCCCGGGAT
    TCCTCTCCGA GCCGCGACTA CACGCCGGCA GTGAGCAGCC TCCGATCCCC CATTTGCATC
    CAACGCTCCG GGAAAAAATA CGGTTTCACC TTGCGGGCCA TCCGGGTGTA CATGGGGGAA
    AGTGATGTGT ACAGCGTGCA GCACATTGTC TGGCACGTGG AGGAAGGAGG GCCTGCCCAT
    GAAGCGGGTC TCTGTGCCGG GGACCTCATT ACACACATTA ACGGGGAGCC GGTGCACGGC
    CTGGTGCACA CTGAAGTTGT GGAGCTGATT TTAAAGAGCG GTAACAAGGT GTTAGTAACC
    ACCACCCCCT TTGAGAACAC CTCGATCCGA ACCGGCCCAG CCAGGAGAAG CAGTTATCGA
    TCCAAAATGG CGCGGAGGAA CAAGCGGATC GCTGGGAGAG AGGGGCAGGA GAGTAAGAAG
    CGGAGCTCTC TATTCCGTAA GATCACCAAG CAATCCAATC TTCTGCACAC CAGCCGTTCC
    CTCTCCTCTC TGAGCCGCTC CCTCTCCTCA AGTGACAGTC TTCCAGGATC ACCCACTCAC
    AGCCTCTCGG CCCGTTCCCC GACCCATAGC TACCGCTCCA TGGACTCTGC TTACCTTGGT
    GCTTCTTCGC AGGGCAGTTC CCCCTCTTCC AGTACCCCTA ATTCCCCTGC ACCACCACCT
    CCTGCCTCAG CCTCTTCCTG TGCGTCAGCC TCCTCCTCCT CCACCACTCC TGCTCCCCCC
    TCCCCACACA TCCGACCCAG CACCCTGCAT GGCTTATCTC CCAAACTGCA CCGCCAGTAT
    CGCTCAGCGC GATGTAAATC TGCGGGTAAT ATGCCCCTCT CTCCCCTTGC ACACACCCCT
    TCCCCTACTG CAGCTGCCTC ACCCCCTCTC CTACCAAGTC ACAGCATTGG CAGCAGCCTG
    ACCACTCAGA GCTTTCCCCC AAAACTGCAT CCATCTCCAC CAGTGTCACG CCCTCGCCCA
    AAGAGCGCAG AGCCTCCACG GTCCCCACTT CTTAAGAGAG TGCAGTCTGC AGAGAAACTG
    GGTGGGGGAG GAGGTGGTGG GTCACCCAGA AAACAGGAGC CGGGAGGGGG AGGAACAATA
    GAGGGAGATT TAGCAGAAGC CATTGGTGTG AGGAGCCAGC CAGTAAGGAG AGTGGGTAGG
    CAGGAGTCTC CTCGCAGTCT TTGTGGAGGG GAAAATATGA TAGGAGAGAA CTTTGAGAGG
    CTGGAAGGAG AAGGAGCGAT AGGACTATCG CCGCTAGGAG ACAGTCTGAA ATGTAGTGAG
    GGTAAAGTCC AAGCAGCCGT TAAGGTTGGA GTAGGATCAA GGACTACTCC CCCATTTTCT
    CCCCAAGAAC CTCCAGCCGC ATCAACTGCA GTCCTTCCCC CCCAAAGTGA CCCTTCCCTT
    GCTATGGTAG GAAGGTGCCA AGTGAAAACC CTAAGTCCTG TACAAGAACA TGAAGGTAGA
    AGGGGGGAAG GAGAGGACGG GGGAGCAGAG GGAGAGGGAA GAAAGGGAGA TTATGAGGAG
    GAACAAGGAA GAACAAGAGA ACAATGGTAC TTAGAGGTGG TAGAAGAGTT GACTACAGTA
    GGGAAAAGCA GCATGCATGC TGGGAGTAAA GCGACTCCCT CTAACCTCAC AGCCGACTCT
    AGTATCTACC ATTCCCACTC AAATAAGAGT AAAGTCGCTG TGGTTGACTC TATGAAGAGC
    ACACCCTCTC ACCCTACAGG TACAACTCTG GAGCAGTCTC TTTCTTTGAA AGGGATTAGA
    TCTGATGCCG ACTCTAAAAA GGGGCAAGAT CCTAAAAACT GCCAGATTAC TCCTCTTCTT
    CCCGTAAGGG AGGGCCTAAA TGTTGAACCA ATTCAAAATG GTTCTACTCA CCCTCCTGAA
    CTTTCCCACG TGGCCAAGGA GCAAAGTATC GACCATCTAA AAGGTCCATC ATCTTCTGGC
    CAACAAATGA AAAGCACTAG CACTTTCCTA CCTACTCAGC TTCACCCAAC CACTGGTAGA
    AAGCTGGAGG CTGATAATGT AAGTAGGCAT TTACTCTGTA CAGAGGGTAC AAAGTATGGT
    CTGATTAAAC CAACAGATTC TCTGTCTATT AGTATTAAAG GGGAGAAGGC CAGTTTAGCA
    AAAAGCCCCC AGGATGATCC AGTAAAGGCC GCTGATCATA CTACTGGGGT GCAAGGACCT
    GCTCCTGCAA AAGGACCAGC AATTTCACCT GTAAATATTA AAATCGGCAG AGAAGGATCT
    GAACAGACAA GGGTGACCAC TACTGACCCA ACTATTAATA GGCTGAGCGA AACACAAACT
    AATCAGAGAC CTGCAGCTGC CACTGGTCTT GCTAGCAATA TGGTACTTAG GGAGATGGAG
    AAGAAGGAAG AGTTCAGTTC AGCCCAGAAT ATAAAGAAAC CAAGTGGAGC AGAATTAGAA
    CAGAAGCGGG TGGCCATTGT AGTCCCGATT GTAACTAGAC CCAGTGATGT AAAGGCTGAC
    ACAACAAGGG TAGTATCTGC TCCCCTGACT GTTAATAGAC CTGCCGCAGG AGAGAGTGAC
    CAAGCAAAAG TGACCACTGT TACCCCAACT ATTGATAAAG TCAGTGGCCC AGAAAGTGCC
    TCTGCCAAGG TGACCGCCGT TGCCTCAATG AGTAGCGCTA ACAAAGCTGT TAATGAACCA
    CCAAGCCTGT TCCCCGATAA TAAAGTAGCC GACTCCAATC AAATAAGGTT CTCGTCACAG
    AATAACAAGG GCGCGAGAGA ATCAGAAAGT GACCAGATAA GGGCAACGGG CAATATTCCC
    GTTATTAACC AGACCGCTCA AACAACGGTT CCTTCCCCAA CACCCACAAA TGGCCAAGGA
    CCCCCTATAA TTATCCGAGT TAGTGGGGTT CCATGTGATG CCCAGGGCGG GCTGTGTCCT
    GAGAAACCTT CCAGCCCCCA AAGAGCGCCC ACCACTACCA GCCAACCCCC TTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755308.1
    CDS430..6066
    Translation

    Target ORF information:

    RefSeq Version XM_031899448.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis microtubule associated serine/threonine kinase 1 (mast1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899448.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGTTCCGCC GCACCAAGAG TTGCAGAAGC AGCAACAGAA AGAGTTTAAT CCTCACAAGC 
    AGCAGTTCCC CCACTCTCCC CCGGCCCCAC TCCCCCCTGC CGGGGCATAT AGGTGGGAGC
    CCTATGGACA GTCCCAGGAA CTTCTCCCCC AGCGCCGCTG CTCACTTCTC CTTTGCATCA
    TCTCGCAGCA GAACCGATGG CCGTCGCTGG TCCTTGGCCT CCCTCCCGTC GTCCGGCTAT
    GGGACAAATA CCCCGAGTTC CACGGTGTCG TCCTCCTCCT CCTCTCAGGA ACGCCTACAC
    CAGCTCCCCT TCCAGCCCAC ACTGGATGAG CTCCACTTCC TTTCCAAACA CTTTGGCAGC
    ACAGAAAGTA TCCCCGATGA TGATGGGGGG CGCAGGTCAC CTGCGGTCAG ACCACGATCC
    CGCAGCTTAA GTCCTGGCCG ATCTCCCTCC TCTTATGACA ATGAAATTGT GATGATGAAC
    CATGTTTATA AAGAGAGGTT TCCCAAGGCC ACAGCGCAGA TGGAGGATAG GCTACGGATG
    TTCATCTCCT CCTTCTCCCC CGAGAATGTC CTACCACTGG CGGACGGACT CCTCAACTTT
    ATCCACCATC AGATCGTGGA GCTGGCGAGG GACTGCCTGA ACAGGAGCAC CGAGGGCATG
    ATCACAACCG CCCACTTTTA CCAACTACAG GAGAATCTGG AGAAGCTGCT GAATGATGCA
    GTGGAACGTT CTTCAGAGGG ATCAGAAGTT GCCTTCATTG CTCAACTGAT GAAAAATCTT
    TTAATCATCA TCTCTCGACC TGCTCGTCTG CTGGAGTGTC TGGAATTTAA CCCTGAAGAG
    TTCTATCACA TGCTGGAGGT AGCCGAGGAC CAGGTGAAAG AAGGAAACTT GAAAAAAGAC
    ATACCCCAAT ACATCCTGAG ACAGTTGGGA TTAACCAGGG ATTCCTTCCC AGAGGTAGCC
    GCTCCCGAGG AACCAGTGAC CCTGGAAGCA CAAACCTCAG AAGCAGAGGA CAACGTAGAG
    GCAAAGAGTG GGGCGGCCCA TAAACCCATC AGACCACCCG AGGAAAGTGA CTTTGAAAAT
    ATAAAGCTCA TCAGTAACGG AGCCTATGGA GCGGTGTATT TGGTGCGTCA CCGGGAGACG
    CGGCAACGCT TTGCCATGAA GAAGATCAAC AAGCAGAACT TGCGTCTGAG GAAACAAGTC
    CACCAGGCCT TTGTAGAACG GGACATTCTG ACCTTTGCTC AGAACCCCTT TGTGGTCAGC
    ATGTTCTGCT CCTTCCAGAC CCGTAGGCAC CTCTGTATGG TGATGGAGTA TGTGGAAGGG
    GGTGACTGTG CCACCCTACT GAAGCACATT GGTGCTCTCC CATTGGAAAT GGCGCGGTTA
    TATTTCGCAG AGACGGTTCT AGCTCTGGAA TACCTACATA ATTATGGGAT TGTGCACCGA
    GATCTGAAAC CGGACAATCT CCTAATCACT TCGATGGGTC ACATAAAGCT TACAGATTTC
    GGCCTGTCCA AGATGGGGCT TATGAACCTC ACCACTAATC TCTATGAAGG ACACATCGAG
    AGCGAAGCAA GGGAATTCCT GGACAAGCAG GTCTGTGGAA CCCCTGAATA CATCGCACCG
    GAGGTTATCC TTAGACAAGG TTACGGGAAG CCAGTGGACT GGTGGGCGAT GGGCATAATT
    CTGTATGAGT TCCTGGTTGG TTGCGTGCCA TTTTTTGGAG ACACCCCAGA GGAGCTGTTT
    GGTCAGGTGA TCAGTGATGA CATTGTGTGG CCAGAAGCAG AGGAAGCTCT ACCCGCCGAT
    GCCCAAAATC TCATCTCCTG CCTCCTGCAG ACAGATCCCT TACAAAGGCT TGGAACAGGG
    GGAGCCATTG AGGTGAAAAT TCACAGATTT TTCACTGATT TGGACTGGAA TGGATTGCTC
    AGGCAGAAAG CTGAATTCAT CCCTCACTTG GAGGCAGAAG ATGATACCAG TTACTTTGAC
    ACTCGATCGG ATCGGTACCA GCACATTAAT TCCTACGATG AGGATGATAC CAATGATGAA
    GACTCGGTGG AGATCAGGCA GTTCTCATCC TGCACCCCAA GATTTAGTAA GGTGTATAGT
    AGTATGGAGC AGCTCTCCCA GCTGGAGCAG AAACCCCCCA CTCCTCCTAG AGACACCAAC
    AAACAAGAAA GGTCTGAGAA GACGACCAAG AGAGAGACGT TGGGGGGCGT CACCCTTCGA
    GACCGCTGCT GGAGGACCGT GTCTCCTGAA ATGAAACAAT TCTCCGATGC GGCAGACGCA
    GAATCCAGCC CCCCCTTGGC TGCCCGGCGG CGCTTTTCTG CCTTAGTGGA CCCGACCCGC
    TATACGTCTC CCCTGGAGTA TGAGGTGGAG TACAGAGTGA GGGGAAGCAG CGGGCCGCTG
    CCCGCCCAAA GCAAGATCCC TCTGGGAGAG CTGGAGCCAG TAGACAAGTC TGTCGACTCT
    CAGCCCCACC AGGAGGCGGA TTCCTCTCCT CGCGCCACAA ACGAGATTGT CCTGCGCAGA
    GCCAGGCACC AACACCTGTC CGGGGACACC GAGAGGAGGA GAACCGGCGG GAAAGTCATC
    AAGTCGGCAT CGACCACCGC CCTGTCTGTC ATGATCCCTA CAGTTGAACA GCACAGCAGC
    TCTCCCCTGG TCAGCCCCAT GTCCCCCCGC TCTCTGTCGT CCAATCCCTC CTCCCGGGAT
    TCCTCTCCGA GCCGCGACTA CACGCCGGCA GTGAGCAGCC TCCGATCCCC CATTTGCATC
    CAACGCTCCG GGAAAAAATA CGGTTTCACC TTGCGGGCCA TCCGGGTGTA CATGGGGGAA
    AGTGATGTGT ACAGCGTGCA GCACATTGTC TGGCACGTGG AGGAAGGAGG GCCTGCCCAT
    GAAGCGGGTC TCTGTGCCGG GGACCTCATT ACACACATTA ACGGGGAGCC GGTGCACGGC
    CTGGTGCACA CTGAAGTTGT GGAGCTGATT TTAAAGAGCG GTAACAAGGT GTTAGTAACC
    ACCACCCCCT TTGAGAACAC CTCGATCCGA ACCGGCCCAG CCAGGAGAAG CAGTTATCGA
    TCCAAAATGG CGCGGAGGAA CAAGCGGATC GCTGGGAGAG AGGGGCAGGA GAGTAAGAAG
    CGGAGCTCTC TATTCCGTAA GATCACCAAG CAATCCAATC TTCTGCACAC CAGCCGTTCC
    CTCTCCTCTC TGAGCCGCTC CCTCTCCTCA AGTGACAGTC TTCCAGGATC ACCCACTCAC
    AGCCTCTCGG CCCGTTCCCC GACCCATAGC TACCGCTCCA TGGACTCTGC TTACCTTGGT
    GCTTCTTCGC AGGGCAGTTC CCCCTCTTCC AGTACCCCTA ATTCCCCTGC ACCACCACCT
    CCTGCCTCAG CCTCTTCCTG TGCGTCAGCC TCCTCCTCCT CCACCACTCC TGCTCCCCCC
    TCCCCACACA TCCGACCCAG CACCCTGCAT GGCTTATCTC CCAAACTGCA CCGCCAGTAT
    CGCTCAGCGC GATGTAAATC TGCGGGTAAT ATGCCCCTCT CTCCCCTTGC ACACACCCCT
    TCCCCTACTG CAGCTGCCTC ACCCCCTCTC CTACCAAGTC ACAGCATTGG CAGCAGCCTG
    ACCACTCAGA GCTTTCCCCC AAAACTGCAT CCATCTCCAC CAGTGTCACG CCCTCGCCCA
    AAGAGCGCAG AGCCTCCACG GTCCCCACTT CTTAAGAGAG TGCAGTCTGC AGAGAAACTG
    GGTGGGGGAG GAGGTGGTGG GTCACCCAGA AAACAGGAGC CGGGAGGGGG AGGAACAATA
    GAGGGAGATT TAGCAGAAGC CATTGGTGTG AGGAGCCAGC CAGTAAGGAG AGTGGGTAGG
    CAGGAGTCTC CTCGCAGTCT TTGTGGAGGG GAAAATATGA TAGGAGAGAA CTTTGAGAGG
    CTGGAAGGAG AAGGAGCGAT AGGACTATCG CCGCTAGGAG ACAGTCTGAA ATGTAGTGAG
    GGTAAAGTCC AAGCAGCCGT TAAGGTTGGA GTAGGATCAA GGACTACTCC CCCATTTTCT
    CCCCAAGAAC CTCCAGCCGC ATCAACTGCA GTCCTTCCCC CCCAAAGTGA CCCTTCCCTT
    GCTATGGTAG GAAGGTGCCA AGTGAAAACC CTAAGTCCTG TACAAGAACA TGAAGGTAGA
    AGGGGGGAAG GAGAGGACGG GGGAGCAGAG GGAGAGGGAA GAAAGGGAGA TTATGAGGAG
    GAACAAGGAA GAACAAGAGA ACAATGGTAC TTAGAGGTGG TAGAAGAGTT GACTACAGTA
    GGGAAAAGCA GCATGCATGC TGGGAGTAAA GCGACTCCCT CTAACCTCAC AGCCGACTCT
    AGTATCTACC ATTCCCACTC AAATAAGAGT AAAGTCGCTG TGGTTGACTC TATGAAGAGC
    ACACCCTCTC ACCCTACAGG TACAACTCTG GAGCAGTCTC TTTCTTTGAA AGGGATTAGA
    TCTGATGCCG ACTCTAAAAA GGGGCAAGAT CCTAAAAACT GCCAGATTAC TCCTCTTCTT
    CCCGTAAGGG AGGGCCTAAA TGTTGAACCA ATTCAAAATG GTTCTACTCA CCCTCCTGAA
    CTTTCCCACG TGGCCAAGGA GCAAAGTATC GACCATCTAA AAGGTCCATC ATCTTCTGGC
    CAACAAATGA AAAGCACTAG CACTTTCCTA CCTACTCAGC TTCACCCAAC CACTGGTAGA
    AAGCTGGAGG CTGATAATGT AAGTAGGCAT TTACTCTGTA CAGAGGGTAC AAAGTATGGT
    CTGATTAAAC CAACAGATTC TCTGTCTATT AGTATTAAAG GGGAGAAGGC CAGTTTAGCA
    AAAAGCCCCC AGGATGATCC AGTAAAGGCC GCTGATCATA CTACTGGGGT GCAAGGACCT
    GCTCCTGCAA AAGGACCAGC AATTTCACCT GTAAATATTA AAATCGGCAG AGAAGGATCT
    GAACAGACAA GGGTGACCAC TACTGACCCA ACTATTAATA GGCTGAGCGA AACACAAACT
    AATCAGAGAC CTGCAGCTGC CACTGGTCTT GCTAGCAATA TGGTACTTAG GGAGATGGAG
    AAGAAGGAAG AGTTCAGTTC AGCCCAGAAT ATAAAGAAAC CAAGTGGAGC AGAATTAGAA
    CAGAAGCGGG TGGCCATTGT AGTCCCGATT GTAACTAGAC CCAGTGATGT AAAGGCTGAC
    ACAACAAGGG TAGTATCTGC TCCCCTGACT GTTAATAGAC CTGCCGCAGG AGAGAGTGAC
    CAAGCAAAAG TGACCACTGT TACCCCAACT ATTGATAAAG TCAGTGGCCC AGAAAGTGCC
    TCTGCCAAGG TGACCGCCGT TGCCTCAATG AGTAGCGCTA ACAAAGCTGT TAATGAACCA
    CCAAGCCTGT TCCCCGATAA TAAAGTAGCC GACTCCAATC AAATAAGGTT CTCGTCACAG
    AATAACAAGG GCGCGAGAGA ATCAGAAAGT GACCAGATAA GGGCAACGGG CAATATTCCC
    GTTATTAACC AGACCGCTCA AACAACGGTT CCTTCCCCAA CACCCACAAA TGGCCAAGGA
    CCCCCTATAA TTATCCGAGT TAGTGGGGTT CCATGTGATG CCCAGGGCGG GCTGTGTCCT
    GAGAAACCTT CCAGCCCCCA AAGAGCGCCC ACCACTACCA GCCAACCCCC TTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55400
    Clone ID Related Accession (Same CDS sequence) XM_031899449.1
    Accession Version XM_031899449.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5634bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product microtubule-associated serine/threonine-protein kinase 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGTTCCGCC GCACCAAGAG TTGCAGAAGC AGCAACAGAA AGAGTTTAAT CCTCACAAGC 
    AGCAGTTCCC CCACTCTCCC CCGGCCCCAC TCCCCCCTGC CGGGGCATAT AGGTGGGAGC
    CCTATGGACA GTCCCAGGAA CTTCTCCCCC AGCGCCGCTG CTCACTTCTC CTTTGCATCA
    TCTCGCAGAA CCGATGGCCG TCGCTGGTCC TTGGCCTCCC TCCCGTCGTC CGGCTATGGG
    ACAAATACCC CGAGTTCCAC GGTGTCGTCC TCCTCCTCCT CTCAGGAACG CCTACACCAG
    CTCCCCTTCC AGCCCACACT GGATGAGCTC CACTTCCTTT CCAAACACTT TGGCAGCACA
    GAAAGTATCC CCGATGATGA TGGGGGGCGC AGGTCACCTG CGGTCAGACC ACGATCCCGC
    AGCTTAAGTC CTGGCCGATC TCCCTCCTCT TATGACAATG AAATTGTGAT GATGAACCAT
    GTTTATAAAG AGAGGTTTCC CAAGGCCACA GCGCAGATGG AGGATAGGCT ACGGATGTTC
    ATCTCCTCCT TCTCCCCCGA GAATGTCCTA CCACTGGCGG ACGGACTCCT CAACTTTATC
    CACCATCAGA TCGTGGAGCT GGCGAGGGAC TGCCTGAACA GGAGCACCGA GGGCATGATC
    ACAACCGCCC ACTTTTACCA ACTACAGGAG AATCTGGAGA AGCTGCTGAA TGATGCAGTG
    GAACGTTCTT CAGAGGGATC AGAAGTTGCC TTCATTGCTC AACTGATGAA AAATCTTTTA
    ATCATCATCT CTCGACCTGC TCGTCTGCTG GAGTGTCTGG AATTTAACCC TGAAGAGTTC
    TATCACATGC TGGAGGTAGC CGAGGACCAG GTGAAAGAAG GAAACTTGAA AAAAGACATA
    CCCCAATACA TCCTGAGACA GTTGGGATTA ACCAGGGATT CCTTCCCAGA GGTAGCCGCT
    CCCGAGGAAC CAGTGACCCT GGAAGCACAA ACCTCAGAAG CAGAGGACAA CGTAGAGGCA
    AAGAGTGGGG CGGCCCATAA ACCCATCAGA CCACCCGAGG AAAGTGACTT TGAAAATATA
    AAGCTCATCA GTAACGGAGC CTATGGAGCG GTGTATTTGG TGCGTCACCG GGAGACGCGG
    CAACGCTTTG CCATGAAGAA GATCAACAAG CAGAACTTGC GTCTGAGGAA ACAAGTCCAC
    CAGGCCTTTG TAGAACGGGA CATTCTGACC TTTGCTCAGA ACCCCTTTGT GGTCAGCATG
    TTCTGCTCCT TCCAGACCCG TAGGCACCTC TGTATGGTGA TGGAGTATGT GGAAGGGGGT
    GACTGTGCCA CCCTACTGAA GCACATTGGT GCTCTCCCAT TGGAAATGGC GCGGTTATAT
    TTCGCAGAGA CGGTTCTAGC TCTGGAATAC CTACATAATT ATGGGATTGT GCACCGAGAT
    CTGAAACCGG ACAATCTCCT AATCACTTCG ATGGGTCACA TAAAGCTTAC AGATTTCGGC
    CTGTCCAAGA TGGGGCTTAT GAACCTCACC ACTAATCTCT ATGAAGGACA CATCGAGAGC
    GAAGCAAGGG AATTCCTGGA CAAGCAGGTC TGTGGAACCC CTGAATACAT CGCACCGGAG
    GTTATCCTTA GACAAGGTTA CGGGAAGCCA GTGGACTGGT GGGCGATGGG CATAATTCTG
    TATGAGTTCC TGGTTGGTTG CGTGCCATTT TTTGGAGACA CCCCAGAGGA GCTGTTTGGT
    CAGGTGATCA GTGATGACAT TGTGTGGCCA GAAGCAGAGG AAGCTCTACC CGCCGATGCC
    CAAAATCTCA TCTCCTGCCT CCTGCAGACA GATCCCTTAC AAAGGCTTGG AACAGGGGGA
    GCCATTGAGG TGAAAATTCA CAGATTTTTC ACTGATTTGG ACTGGAATGG ATTGCTCAGG
    CAGAAAGCTG AATTCATCCC TCACTTGGAG GCAGAAGATG ATACCAGTTA CTTTGACACT
    CGATCGGATC GGTACCAGCA CATTAATTCC TACGATGAGG ATGATACCAA TGATGAAGAC
    TCGGTGGAGA TCAGGCAGTT CTCATCCTGC ACCCCAAGAT TTAGTAAGGT GTATAGTAGT
    ATGGAGCAGC TCTCCCAGCT GGAGCAGAAA CCCCCCACTC CTCCTAGAGA CACCAACAAA
    CAAGAAAGGT CTGAGAAGAC GACCAAGAGA GAGACGTTGG GGGGCGTCAC CCTTCGAGAC
    CGCTGCTGGA GGACCGTGTC TCCTGAAATG AAACAATTCT CCGATGCGGC AGACGCAGAA
    TCCAGCCCCC CCTTGGCTGC CCGGCGGCGC TTTTCTGCCT TAGTGGACCC GACCCGCTAT
    ACGTCTCCCC TGGAGTATGA GGTGGAGTAC AGAGTGAGGG GAAGCAGCGG GCCGCTGCCC
    GCCCAAAGCA AGATCCCTCT GGGAGAGCTG GAGCCAGTAG ACAAGTCTGT CGACTCTCAG
    CCCCACCAGG AGGCGGATTC CTCTCCTCGC GCCACAAACG AGATTGTCCT GCGCAGAGCC
    AGGCACCAAC ACCTGTCCGG GGACACCGAG AGGAGGAGAA CCGGCGGGAA AGTCATCAAG
    TCGGCATCGA CCACCGCCCT GTCTGTCATG ATCCCTACAG TTGAACAGCA CAGCAGCTCT
    CCCCTGGTCA GCCCCATGTC CCCCCGCTCT CTGTCGTCCA ATCCCTCCTC CCGGGATTCC
    TCTCCGAGCC GCGACTACAC GCCGGCAGTG AGCAGCCTCC GATCCCCCAT TTGCATCCAA
    CGCTCCGGGA AAAAATACGG TTTCACCTTG CGGGCCATCC GGGTGTACAT GGGGGAAAGT
    GATGTGTACA GCGTGCAGCA CATTGTCTGG CACGTGGAGG AAGGAGGGCC TGCCCATGAA
    GCGGGTCTCT GTGCCGGGGA CCTCATTACA CACATTAACG GGGAGCCGGT GCACGGCCTG
    GTGCACACTG AAGTTGTGGA GCTGATTTTA AAGAGCGGTA ACAAGGTGTT AGTAACCACC
    ACCCCCTTTG AGAACACCTC GATCCGAACC GGCCCAGCCA GGAGAAGCAG TTATCGATCC
    AAAATGGCGC GGAGGAACAA GCGGATCGCT GGGAGAGAGG GGCAGGAGAG TAAGAAGCGG
    AGCTCTCTAT TCCGTAAGAT CACCAAGCAA TCCAATCTTC TGCACACCAG CCGTTCCCTC
    TCCTCTCTGA GCCGCTCCCT CTCCTCAAGT GACAGTCTTC CAGGATCACC CACTCACAGC
    CTCTCGGCCC GTTCCCCGAC CCATAGCTAC CGCTCCATGG ACTCTGCTTA CCTTGGTGCT
    TCTTCGCAGG GCAGTTCCCC CTCTTCCAGT ACCCCTAATT CCCCTGCACC ACCACCTCCT
    GCCTCAGCCT CTTCCTGTGC GTCAGCCTCC TCCTCCTCCA CCACTCCTGC TCCCCCCTCC
    CCACACATCC GACCCAGCAC CCTGCATGGC TTATCTCCCA AACTGCACCG CCAGTATCGC
    TCAGCGCGAT GTAAATCTGC GGGTAATATG CCCCTCTCTC CCCTTGCACA CACCCCTTCC
    CCTACTGCAG CTGCCTCACC CCCTCTCCTA CCAAGTCACA GCATTGGCAG CAGCCTGACC
    ACTCAGAGCT TTCCCCCAAA ACTGCATCCA TCTCCACCAG TGTCACGCCC TCGCCCAAAG
    AGCGCAGAGC CTCCACGGTC CCCACTTCTT AAGAGAGTGC AGTCTGCAGA GAAACTGGGT
    GGGGGAGGAG GTGGTGGGTC ACCCAGAAAA CAGGAGCCGG GAGGGGGAGG AACAATAGAG
    GGAGATTTAG CAGAAGCCAT TGGTGTGAGG AGCCAGCCAG TAAGGAGAGT GGGTAGGCAG
    GAGTCTCCTC GCAGTCTTTG TGGAGGGGAA AATATGATAG GAGAGAACTT TGAGAGGCTG
    GAAGGAGAAG GAGCGATAGG ACTATCGCCG CTAGGAGACA GTCTGAAATG TAGTGAGGGT
    AAAGTCCAAG CAGCCGTTAA GGTTGGAGTA GGATCAAGGA CTACTCCCCC ATTTTCTCCC
    CAAGAACCTC CAGCCGCATC AACTGCAGTC CTTCCCCCCC AAAGTGACCC TTCCCTTGCT
    ATGGTAGGAA GGTGCCAAGT GAAAACCCTA AGTCCTGTAC AAGAACATGA AGGTAGAAGG
    GGGGAAGGAG AGGACGGGGG AGCAGAGGGA GAGGGAAGAA AGGGAGATTA TGAGGAGGAA
    CAAGGAAGAA CAAGAGAACA ATGGTACTTA GAGGTGGTAG AAGAGTTGAC TACAGTAGGG
    AAAAGCAGCA TGCATGCTGG GAGTAAAGCG ACTCCCTCTA ACCTCACAGC CGACTCTAGT
    ATCTACCATT CCCACTCAAA TAAGAGTAAA GTCGCTGTGG TTGACTCTAT GAAGAGCACA
    CCCTCTCACC CTACAGGTAC AACTCTGGAG CAGTCTCTTT CTTTGAAAGG GATTAGATCT
    GATGCCGACT CTAAAAAGGG GCAAGATCCT AAAAACTGCC AGATTACTCC TCTTCTTCCC
    GTAAGGGAGG GCCTAAATGT TGAACCAATT CAAAATGGTT CTACTCACCC TCCTGAACTT
    TCCCACGTGG CCAAGGAGCA AAGTATCGAC CATCTAAAAG GTCCATCATC TTCTGGCCAA
    CAAATGAAAA GCACTAGCAC TTTCCTACCT ACTCAGCTTC ACCCAACCAC TGGTAGAAAG
    CTGGAGGCTG ATAATGTAAG TAGGCATTTA CTCTGTACAG AGGGTACAAA GTATGGTCTG
    ATTAAACCAA CAGATTCTCT GTCTATTAGT ATTAAAGGGG AGAAGGCCAG TTTAGCAAAA
    AGCCCCCAGG ATGATCCAGT AAAGGCCGCT GATCATACTA CTGGGGTGCA AGGACCTGCT
    CCTGCAAAAG GACCAGCAAT TTCACCTGTA AATATTAAAA TCGGCAGAGA AGGATCTGAA
    CAGACAAGGG TGACCACTAC TGACCCAACT ATTAATAGGC TGAGCGAAAC ACAAACTAAT
    CAGAGACCTG CAGCTGCCAC TGGTCTTGCT AGCAATATGG TACTTAGGGA GATGGAGAAG
    AAGGAAGAGT TCAGTTCAGC CCAGAATATA AAGAAACCAA GTGGAGCAGA ATTAGAACAG
    AAGCGGGTGG CCATTGTAGT CCCGATTGTA ACTAGACCCA GTGATGTAAA GGCTGACACA
    ACAAGGGTAG TATCTGCTCC CCTGACTGTT AATAGACCTG CCGCAGGAGA GAGTGACCAA
    GCAAAAGTGA CCACTGTTAC CCCAACTATT GATAAAGTCA GTGGCCCAGA AAGTGCCTCT
    GCCAAGGTGA CCGCCGTTGC CTCAATGAGT AGCGCTAACA AAGCTGTTAA TGAACCACCA
    AGCCTGTTCC CCGATAATAA AGTAGCCGAC TCCAATCAAA TAAGGTTCTC GTCACAGAAT
    AACAAGGGCG CGAGAGAATC AGAAAGTGAC CAGATAAGGG CAACGGGCAA TATTCCCGTT
    ATTAACCAGA CCGCTCAAAC AACGGTTCCT TCCCCAACAC CCACAAATGG CCAAGGACCC
    CCTATAATTA TCCGAGTTAG TGGGGTTCCA TGTGATGCCC AGGGCGGGCT GTGTCCTGAG
    AAACCTTCCA GCCCCCAAAG AGCGCCCACC ACTACCAGCC AACCCCCTTC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755309.1
    CDS430..6063
    Translation

    Target ORF information:

    RefSeq Version XM_031899449.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis microtubule associated serine/threonine kinase 1 (mast1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899449.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGTTCCGCC GCACCAAGAG TTGCAGAAGC AGCAACAGAA AGAGTTTAAT CCTCACAAGC 
    AGCAGTTCCC CCACTCTCCC CCGGCCCCAC TCCCCCCTGC CGGGGCATAT AGGTGGGAGC
    CCTATGGACA GTCCCAGGAA CTTCTCCCCC AGCGCCGCTG CTCACTTCTC CTTTGCATCA
    TCTCGCAGAA CCGATGGCCG TCGCTGGTCC TTGGCCTCCC TCCCGTCGTC CGGCTATGGG
    ACAAATACCC CGAGTTCCAC GGTGTCGTCC TCCTCCTCCT CTCAGGAACG CCTACACCAG
    CTCCCCTTCC AGCCCACACT GGATGAGCTC CACTTCCTTT CCAAACACTT TGGCAGCACA
    GAAAGTATCC CCGATGATGA TGGGGGGCGC AGGTCACCTG CGGTCAGACC ACGATCCCGC
    AGCTTAAGTC CTGGCCGATC TCCCTCCTCT TATGACAATG AAATTGTGAT GATGAACCAT
    GTTTATAAAG AGAGGTTTCC CAAGGCCACA GCGCAGATGG AGGATAGGCT ACGGATGTTC
    ATCTCCTCCT TCTCCCCCGA GAATGTCCTA CCACTGGCGG ACGGACTCCT CAACTTTATC
    CACCATCAGA TCGTGGAGCT GGCGAGGGAC TGCCTGAACA GGAGCACCGA GGGCATGATC
    ACAACCGCCC ACTTTTACCA ACTACAGGAG AATCTGGAGA AGCTGCTGAA TGATGCAGTG
    GAACGTTCTT CAGAGGGATC AGAAGTTGCC TTCATTGCTC AACTGATGAA AAATCTTTTA
    ATCATCATCT CTCGACCTGC TCGTCTGCTG GAGTGTCTGG AATTTAACCC TGAAGAGTTC
    TATCACATGC TGGAGGTAGC CGAGGACCAG GTGAAAGAAG GAAACTTGAA AAAAGACATA
    CCCCAATACA TCCTGAGACA GTTGGGATTA ACCAGGGATT CCTTCCCAGA GGTAGCCGCT
    CCCGAGGAAC CAGTGACCCT GGAAGCACAA ACCTCAGAAG CAGAGGACAA CGTAGAGGCA
    AAGAGTGGGG CGGCCCATAA ACCCATCAGA CCACCCGAGG AAAGTGACTT TGAAAATATA
    AAGCTCATCA GTAACGGAGC CTATGGAGCG GTGTATTTGG TGCGTCACCG GGAGACGCGG
    CAACGCTTTG CCATGAAGAA GATCAACAAG CAGAACTTGC GTCTGAGGAA ACAAGTCCAC
    CAGGCCTTTG TAGAACGGGA CATTCTGACC TTTGCTCAGA ACCCCTTTGT GGTCAGCATG
    TTCTGCTCCT TCCAGACCCG TAGGCACCTC TGTATGGTGA TGGAGTATGT GGAAGGGGGT
    GACTGTGCCA CCCTACTGAA GCACATTGGT GCTCTCCCAT TGGAAATGGC GCGGTTATAT
    TTCGCAGAGA CGGTTCTAGC TCTGGAATAC CTACATAATT ATGGGATTGT GCACCGAGAT
    CTGAAACCGG ACAATCTCCT AATCACTTCG ATGGGTCACA TAAAGCTTAC AGATTTCGGC
    CTGTCCAAGA TGGGGCTTAT GAACCTCACC ACTAATCTCT ATGAAGGACA CATCGAGAGC
    GAAGCAAGGG AATTCCTGGA CAAGCAGGTC TGTGGAACCC CTGAATACAT CGCACCGGAG
    GTTATCCTTA GACAAGGTTA CGGGAAGCCA GTGGACTGGT GGGCGATGGG CATAATTCTG
    TATGAGTTCC TGGTTGGTTG CGTGCCATTT TTTGGAGACA CCCCAGAGGA GCTGTTTGGT
    CAGGTGATCA GTGATGACAT TGTGTGGCCA GAAGCAGAGG AAGCTCTACC CGCCGATGCC
    CAAAATCTCA TCTCCTGCCT CCTGCAGACA GATCCCTTAC AAAGGCTTGG AACAGGGGGA
    GCCATTGAGG TGAAAATTCA CAGATTTTTC ACTGATTTGG ACTGGAATGG ATTGCTCAGG
    CAGAAAGCTG AATTCATCCC TCACTTGGAG GCAGAAGATG ATACCAGTTA CTTTGACACT
    CGATCGGATC GGTACCAGCA CATTAATTCC TACGATGAGG ATGATACCAA TGATGAAGAC
    TCGGTGGAGA TCAGGCAGTT CTCATCCTGC ACCCCAAGAT TTAGTAAGGT GTATAGTAGT
    ATGGAGCAGC TCTCCCAGCT GGAGCAGAAA CCCCCCACTC CTCCTAGAGA CACCAACAAA
    CAAGAAAGGT CTGAGAAGAC GACCAAGAGA GAGACGTTGG GGGGCGTCAC CCTTCGAGAC
    CGCTGCTGGA GGACCGTGTC TCCTGAAATG AAACAATTCT CCGATGCGGC AGACGCAGAA
    TCCAGCCCCC CCTTGGCTGC CCGGCGGCGC TTTTCTGCCT TAGTGGACCC GACCCGCTAT
    ACGTCTCCCC TGGAGTATGA GGTGGAGTAC AGAGTGAGGG GAAGCAGCGG GCCGCTGCCC
    GCCCAAAGCA AGATCCCTCT GGGAGAGCTG GAGCCAGTAG ACAAGTCTGT CGACTCTCAG
    CCCCACCAGG AGGCGGATTC CTCTCCTCGC GCCACAAACG AGATTGTCCT GCGCAGAGCC
    AGGCACCAAC ACCTGTCCGG GGACACCGAG AGGAGGAGAA CCGGCGGGAA AGTCATCAAG
    TCGGCATCGA CCACCGCCCT GTCTGTCATG ATCCCTACAG TTGAACAGCA CAGCAGCTCT
    CCCCTGGTCA GCCCCATGTC CCCCCGCTCT CTGTCGTCCA ATCCCTCCTC CCGGGATTCC
    TCTCCGAGCC GCGACTACAC GCCGGCAGTG AGCAGCCTCC GATCCCCCAT TTGCATCCAA
    CGCTCCGGGA AAAAATACGG TTTCACCTTG CGGGCCATCC GGGTGTACAT GGGGGAAAGT
    GATGTGTACA GCGTGCAGCA CATTGTCTGG CACGTGGAGG AAGGAGGGCC TGCCCATGAA
    GCGGGTCTCT GTGCCGGGGA CCTCATTACA CACATTAACG GGGAGCCGGT GCACGGCCTG
    GTGCACACTG AAGTTGTGGA GCTGATTTTA AAGAGCGGTA ACAAGGTGTT AGTAACCACC
    ACCCCCTTTG AGAACACCTC GATCCGAACC GGCCCAGCCA GGAGAAGCAG TTATCGATCC
    AAAATGGCGC GGAGGAACAA GCGGATCGCT GGGAGAGAGG GGCAGGAGAG TAAGAAGCGG
    AGCTCTCTAT TCCGTAAGAT CACCAAGCAA TCCAATCTTC TGCACACCAG CCGTTCCCTC
    TCCTCTCTGA GCCGCTCCCT CTCCTCAAGT GACAGTCTTC CAGGATCACC CACTCACAGC
    CTCTCGGCCC GTTCCCCGAC CCATAGCTAC CGCTCCATGG ACTCTGCTTA CCTTGGTGCT
    TCTTCGCAGG GCAGTTCCCC CTCTTCCAGT ACCCCTAATT CCCCTGCACC ACCACCTCCT
    GCCTCAGCCT CTTCCTGTGC GTCAGCCTCC TCCTCCTCCA CCACTCCTGC TCCCCCCTCC
    CCACACATCC GACCCAGCAC CCTGCATGGC TTATCTCCCA AACTGCACCG CCAGTATCGC
    TCAGCGCGAT GTAAATCTGC GGGTAATATG CCCCTCTCTC CCCTTGCACA CACCCCTTCC
    CCTACTGCAG CTGCCTCACC CCCTCTCCTA CCAAGTCACA GCATTGGCAG CAGCCTGACC
    ACTCAGAGCT TTCCCCCAAA ACTGCATCCA TCTCCACCAG TGTCACGCCC TCGCCCAAAG
    AGCGCAGAGC CTCCACGGTC CCCACTTCTT AAGAGAGTGC AGTCTGCAGA GAAACTGGGT
    GGGGGAGGAG GTGGTGGGTC ACCCAGAAAA CAGGAGCCGG GAGGGGGAGG AACAATAGAG
    GGAGATTTAG CAGAAGCCAT TGGTGTGAGG AGCCAGCCAG TAAGGAGAGT GGGTAGGCAG
    GAGTCTCCTC GCAGTCTTTG TGGAGGGGAA AATATGATAG GAGAGAACTT TGAGAGGCTG
    GAAGGAGAAG GAGCGATAGG ACTATCGCCG CTAGGAGACA GTCTGAAATG TAGTGAGGGT
    AAAGTCCAAG CAGCCGTTAA GGTTGGAGTA GGATCAAGGA CTACTCCCCC ATTTTCTCCC
    CAAGAACCTC CAGCCGCATC AACTGCAGTC CTTCCCCCCC AAAGTGACCC TTCCCTTGCT
    ATGGTAGGAA GGTGCCAAGT GAAAACCCTA AGTCCTGTAC AAGAACATGA AGGTAGAAGG
    GGGGAAGGAG AGGACGGGGG AGCAGAGGGA GAGGGAAGAA AGGGAGATTA TGAGGAGGAA
    CAAGGAAGAA CAAGAGAACA ATGGTACTTA GAGGTGGTAG AAGAGTTGAC TACAGTAGGG
    AAAAGCAGCA TGCATGCTGG GAGTAAAGCG ACTCCCTCTA ACCTCACAGC CGACTCTAGT
    ATCTACCATT CCCACTCAAA TAAGAGTAAA GTCGCTGTGG TTGACTCTAT GAAGAGCACA
    CCCTCTCACC CTACAGGTAC AACTCTGGAG CAGTCTCTTT CTTTGAAAGG GATTAGATCT
    GATGCCGACT CTAAAAAGGG GCAAGATCCT AAAAACTGCC AGATTACTCC TCTTCTTCCC
    GTAAGGGAGG GCCTAAATGT TGAACCAATT CAAAATGGTT CTACTCACCC TCCTGAACTT
    TCCCACGTGG CCAAGGAGCA AAGTATCGAC CATCTAAAAG GTCCATCATC TTCTGGCCAA
    CAAATGAAAA GCACTAGCAC TTTCCTACCT ACTCAGCTTC ACCCAACCAC TGGTAGAAAG
    CTGGAGGCTG ATAATGTAAG TAGGCATTTA CTCTGTACAG AGGGTACAAA GTATGGTCTG
    ATTAAACCAA CAGATTCTCT GTCTATTAGT ATTAAAGGGG AGAAGGCCAG TTTAGCAAAA
    AGCCCCCAGG ATGATCCAGT AAAGGCCGCT GATCATACTA CTGGGGTGCA AGGACCTGCT
    CCTGCAAAAG GACCAGCAAT TTCACCTGTA AATATTAAAA TCGGCAGAGA AGGATCTGAA
    CAGACAAGGG TGACCACTAC TGACCCAACT ATTAATAGGC TGAGCGAAAC ACAAACTAAT
    CAGAGACCTG CAGCTGCCAC TGGTCTTGCT AGCAATATGG TACTTAGGGA GATGGAGAAG
    AAGGAAGAGT TCAGTTCAGC CCAGAATATA AAGAAACCAA GTGGAGCAGA ATTAGAACAG
    AAGCGGGTGG CCATTGTAGT CCCGATTGTA ACTAGACCCA GTGATGTAAA GGCTGACACA
    ACAAGGGTAG TATCTGCTCC CCTGACTGTT AATAGACCTG CCGCAGGAGA GAGTGACCAA
    GCAAAAGTGA CCACTGTTAC CCCAACTATT GATAAAGTCA GTGGCCCAGA AAGTGCCTCT
    GCCAAGGTGA CCGCCGTTGC CTCAATGAGT AGCGCTAACA AAGCTGTTAA TGAACCACCA
    AGCCTGTTCC CCGATAATAA AGTAGCCGAC TCCAATCAAA TAAGGTTCTC GTCACAGAAT
    AACAAGGGCG CGAGAGAATC AGAAAGTGAC CAGATAAGGG CAACGGGCAA TATTCCCGTT
    ATTAACCAGA CCGCTCAAAC AACGGTTCCT TCCCCAACAC CCACAAATGG CCAAGGACCC
    CCTATAATTA TCCGAGTTAG TGGGGTTCCA TGTGATGCCC AGGGCGGGCT GTGTCCTGAG
    AAACCTTCCA GCCCCCAAAG AGCGCCCACC ACTACCAGCC AACCCCCTTC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.