cacna1g cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following cacna1g gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna1g cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa47120 XM_018097893.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OXa47121 XM_018097894.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OXa51011 XM_031894870.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa51011 XM_031894866.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa51011 XM_031894865.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa51011 XM_031894867.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa51011 XM_031894869.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa51011 XM_031894868.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa51012 XM_031894871.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa51013 XM_031894872.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa51014 XM_004918488.3
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa51015 XM_031894873.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa51016 XM_004918490.3
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa17232 XM_018097891.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OXa17232 XM_018097892.1
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OXa17234 XM_004918488.2
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OXa17236 XM_004918490.2
Latest version!
Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa47120
    Clone ID Related Accession (Same CDS sequence) XM_018097893.1
    Accession Version XM_018097893.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7026bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGATAAAT CTCATGAGCA AAGACCTGCC AAAGACCCTG GGGATCCGGG GTCGGAACTG 
    GAAGGGCTGC CCTACCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ACGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTTCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGAGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATTGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCTTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AATCTGATGC TGGATGATGT AATATTGGAG CACTCAGCCA GTCCAGTTAA AGAGGCCCAG
    TGCAAACCCT ACTACTCGGA GTATTCTCAC CCTCGGCTGC TCATCCATCA AATGTGCACA
    AGCCATTATC TGGATTTGTT TATTACTGGG GTCATCGGAT TGAATGTTAT CACTATGGCG
    ATGGAGCACT ACCAGCAACC CAAGGTATTG GATGAAGCTT TAAAAATCTG TAATTACATA
    TTTACCCTCA TTTTTGTGCT GGAATCTGGG TGCAAAATGG TAGCTTTTGG CTTCCGGCGA
    TTCTTCAAAG ACAGGTGGAA CCAGTTGGAT TTGGCCATTG TTCTGCTCTC CATTATGGGG
    ATCACCCTGG AGGAGATTGA AGTCAATGCA TCGCTGCCCA TTAACCCAAC AATCATCAGA
    ATAATGAGGG TCCTGCGGAT CGCTCGCGTG CTGAAGTTAT TAAAGATGGC AGTCGGTATG
    CGAGCCCTGC TGGATACAGT ATTGCAAGCG CTTCCTCAAG TGGGGAATCT CGGCCTGCTC
    TTTATGCTGC TGTTTTTCAT ATTTGCCGCT TTGGGAGTTG AACTTTTTGG CGACTTAGAA
    TGCACTGATT CCCACCCGTG TGAAGGTCTC GGCAGGCACG CCACGTTCAG GAACTTCGGC
    ATGGCCTTTC TGACTTTGTT CAGAGTATCC ACAGGAGATA ACTGGAACGG TATAATGAAG
    GACACACTCC GTGATTGCAA CGAGGATTCC ACCTGTTACA ACACAGTCAT CTCCCCCATC
    TATTTTGTCT CTTTTGTGCT GACTGCCCAG TTTGTCCTGG TCAATGTGGT AATAGCGGTG
    CTCATGAAAC ACCTTGAGGA AAGCAACAAG GAAGCTAAGG AGGAGGCGGA GCTGGAGGCA
    GAGATGGAAC TAGAGTTTCA GATCCAGAAC ATGCAGGTTC CGGATCCTGA CCCATTTATG
    TGGGCTGGGA GAGATCTCGG CTATCCCCAG GAAGGTTCTT ACGGATATAT GAACTCCGTG
    CAGGACAAGG CCGACTCACA GCTTTCATTA GTCAATCCCA TGGGATCTTT GGAAGGAGAG
    ATGAAGCTGA TGGACAATTT GTCTGGCTCT GTGTGCCACC ATTACGCCCT CCCTCCCTCT
    GACAAGGGCT CCTCAGAAAA ACAGATCCCA TTGGCAGAGA TGGAAGGCCT ATCCATGACA
    TCAGACATTC TGTCCGAGAA ATCATGGTCC TTAGCTCTGA CGGACGACTC CCTGCCAGAT
    GACCCTAATC TCAACCCATT TGGTGTACTG GAAACCAAAC TTGGCCTCCC CGAGGAGAAA
    CATTCGGCAA CCGTTGAGGA GGCTACGAAT CTTCTCAGCG TCCGGAAACC GTCAGTATGC
    CGCACACATT CCCTGCCCAA TGACAGCTAC ATGTTGCAAA CGGGAAGCAC GTGCGCTGAG
    TGCCAGGAGC AAAGAAACCA CTGTTACCAG AAATCAGGTT CCAGCTCAAC GGTTCAGTCT
    CAGCCAAATG ACCTCAACTC TCTTCTCCAA GTGCCACAAA TCTCCATCCC TTATGAAGCT
    TCAGGAAGTA CAGTGATATC AGAGATTCCC AAACTCCCCG CCCCCTTGCG CTCTCCATCG
    GCTAATAGGA TGCTCCGCAG ACAGGAAGCA ATCAGAACCG ACTCTTTGGA ATTTTTTCCC
    GATAGCAAAG AAAATGCTGG CATAACTGAG CCCCCACCTG TATATATTCC AATGTGCCAG
    GTGCCCACCG AGACACCCTC GGCTTTTATA GAACCAGAAA GAACTTTATG CCAGTTGAAG
    CGACGCAGTG TTTACACCCA GCAAGTCCAA GATACCTGCA AGATGTTTAG GTACCCTGAT
    GCTTCTCAAA CCAACATGGA CACGTTACGA GAAACCTCAA AGATTCCAGA GAAAGAAGAT
    TTGTTGAGCG CAGCATGTTG TAGGCGGCAT CTCAACTCCA AAACGTGTAA AGCATTAAGC
    GTCTATTCTG CAGCATACTC CTTAAGCCAG AAACTGAAAC TAGGTAACAG CGGCAACGTT
    ATTTTGAAGG ACATGAAGAG GTTCTACAGC GTAGATGCCC ACGGCGTCCT TAAAAAGACA
    TCCTCTTGGT TGGATGACCC CAGAAGACAT TCCATAGAAA TTTGTTCTTT AGAGAACACT
    CTTCATCACT ACTCTACTTC ACGTTCTTGT GGATTCATTA GCCACGTATT CCAGAAGAAG
    GAGGACTTGC AAATAGTGCA GAGGACGGGC GGACAGGATC CCGTCTGTCC AGATTGTGTT
    CACTCAGGAG AAGCATCAGA ACTACCACTC TCTTACGTCT CGAGTGGGCA ATCACAATCG
    ACAGACTCGA CCGAGCTCGC TAAAATAGAC TCTTTCGTTC CGGAAATGCA GACATCCCCT
    AAACCACTGG GAGATTTAGT TTCTGTCGAA AGTTTTTCTA TGGATGCAGT TGAATTAAAT
    CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017953382.1
    CDS1664..8689
    Translation

    Target ORF information:

    RefSeq Version XM_018097893.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018097893.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGATAAAT CTCATGAGCA AAGACCTGCC AAAGACCCTG GGGATCCGGG GTCGGAACTG 
    GAAGGGCTGC CCTACCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ACGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTTCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGAGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATTGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCTTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AATCTGATGC TGGATGATGT AATATTGGAG CACTCAGCCA GTCCAGTTAA AGAGGCCCAG
    TGCAAACCCT ACTACTCGGA GTATTCTCAC CCTCGGCTGC TCATCCATCA AATGTGCACA
    AGCCATTATC TGGATTTGTT TATTACTGGG GTCATCGGAT TGAATGTTAT CACTATGGCG
    ATGGAGCACT ACCAGCAACC CAAGGTATTG GATGAAGCTT TAAAAATCTG TAATTACATA
    TTTACCCTCA TTTTTGTGCT GGAATCTGGG TGCAAAATGG TAGCTTTTGG CTTCCGGCGA
    TTCTTCAAAG ACAGGTGGAA CCAGTTGGAT TTGGCCATTG TTCTGCTCTC CATTATGGGG
    ATCACCCTGG AGGAGATTGA AGTCAATGCA TCGCTGCCCA TTAACCCAAC AATCATCAGA
    ATAATGAGGG TCCTGCGGAT CGCTCGCGTG CTGAAGTTAT TAAAGATGGC AGTCGGTATG
    CGAGCCCTGC TGGATACAGT ATTGCAAGCG CTTCCTCAAG TGGGGAATCT CGGCCTGCTC
    TTTATGCTGC TGTTTTTCAT ATTTGCCGCT TTGGGAGTTG AACTTTTTGG CGACTTAGAA
    TGCACTGATT CCCACCCGTG TGAAGGTCTC GGCAGGCACG CCACGTTCAG GAACTTCGGC
    ATGGCCTTTC TGACTTTGTT CAGAGTATCC ACAGGAGATA ACTGGAACGG TATAATGAAG
    GACACACTCC GTGATTGCAA CGAGGATTCC ACCTGTTACA ACACAGTCAT CTCCCCCATC
    TATTTTGTCT CTTTTGTGCT GACTGCCCAG TTTGTCCTGG TCAATGTGGT AATAGCGGTG
    CTCATGAAAC ACCTTGAGGA AAGCAACAAG GAAGCTAAGG AGGAGGCGGA GCTGGAGGCA
    GAGATGGAAC TAGAGTTTCA GATCCAGAAC ATGCAGGTTC CGGATCCTGA CCCATTTATG
    TGGGCTGGGA GAGATCTCGG CTATCCCCAG GAAGGTTCTT ACGGATATAT GAACTCCGTG
    CAGGACAAGG CCGACTCACA GCTTTCATTA GTCAATCCCA TGGGATCTTT GGAAGGAGAG
    ATGAAGCTGA TGGACAATTT GTCTGGCTCT GTGTGCCACC ATTACGCCCT CCCTCCCTCT
    GACAAGGGCT CCTCAGAAAA ACAGATCCCA TTGGCAGAGA TGGAAGGCCT ATCCATGACA
    TCAGACATTC TGTCCGAGAA ATCATGGTCC TTAGCTCTGA CGGACGACTC CCTGCCAGAT
    GACCCTAATC TCAACCCATT TGGTGTACTG GAAACCAAAC TTGGCCTCCC CGAGGAGAAA
    CATTCGGCAA CCGTTGAGGA GGCTACGAAT CTTCTCAGCG TCCGGAAACC GTCAGTATGC
    CGCACACATT CCCTGCCCAA TGACAGCTAC ATGTTGCAAA CGGGAAGCAC GTGCGCTGAG
    TGCCAGGAGC AAAGAAACCA CTGTTACCAG AAATCAGGTT CCAGCTCAAC GGTTCAGTCT
    CAGCCAAATG ACCTCAACTC TCTTCTCCAA GTGCCACAAA TCTCCATCCC TTATGAAGCT
    TCAGGAAGTA CAGTGATATC AGAGATTCCC AAACTCCCCG CCCCCTTGCG CTCTCCATCG
    GCTAATAGGA TGCTCCGCAG ACAGGAAGCA ATCAGAACCG ACTCTTTGGA ATTTTTTCCC
    GATAGCAAAG AAAATGCTGG CATAACTGAG CCCCCACCTG TATATATTCC AATGTGCCAG
    GTGCCCACCG AGACACCCTC GGCTTTTATA GAACCAGAAA GAACTTTATG CCAGTTGAAG
    CGACGCAGTG TTTACACCCA GCAAGTCCAA GATACCTGCA AGATGTTTAG GTACCCTGAT
    GCTTCTCAAA CCAACATGGA CACGTTACGA GAAACCTCAA AGATTCCAGA GAAAGAAGAT
    TTGTTGAGCG CAGCATGTTG TAGGCGGCAT CTCAACTCCA AAACGTGTAA AGCATTAAGC
    GTCTATTCTG CAGCATACTC CTTAAGCCAG AAACTGAAAC TAGGTAACAG CGGCAACGTT
    ATTTTGAAGG ACATGAAGAG GTTCTACAGC GTAGATGCCC ACGGCGTCCT TAAAAAGACA
    TCCTCTTGGT TGGATGACCC CAGAAGACAT TCCATAGAAA TTTGTTCTTT AGAGAACACT
    CTTCATCACT ACTCTACTTC ACGTTCTTGT GGATTCATTA GCCACGTATT CCAGAAGAAG
    GAGGACTTGC AAATAGTGCA GAGGACGGGC GGACAGGATC CCGTCTGTCC AGATTGTGTT
    CACTCAGGAG AAGCATCAGA ACTACCACTC TCTTACGTCT CGAGTGGGCA ATCACAATCG
    ACAGACTCGA CCGAGCTCGC TAAAATAGAC TCTTTCGTTC CGGAAATGCA GACATCCCCT
    AAACCACTGG GAGATTTAGT TTCTGTCGAA AGTTTTTCTA TGGATGCAGT TGAATTAAAT
    CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47121
    Clone ID Related Accession (Same CDS sequence) XM_018097894.1
    Accession Version XM_018097894.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGATAAAT CTCATGAGCA AAGACCTGCC AAAGACCCTG GGGATCCGGG GTCGGAACTG 
    GAAGGGCTGC CCTACCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ACGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTTCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGAGTCTTTC CAATGCTTCT
    TCAGCCACAC CCATGCCCAT GCCAAAATGT GCTTTGTTTG GAGATATCCC CATGGCTGGT
    GAATCTAGGA GGGAGAGTAA TGTCTCCGGT GGCCCCCATG CTTGTGAAAT CAGGTCTATG
    TCAAGTTCAA GGAACTCCCC GCACAGCTCC TGGGGTGCAG TGTGTAGTTG GAATAGCCGT
    AGATCTAGTT GGAACAGCAT TGGACGGGCT TCCAGCCTAA AACGGCACGA GCAAAGCGGT
    GAACACAAAT CTTTGCTGTC TGCTGATGGA AAAGAAAGCT CAGAAGGCGA CACATCAGAC
    GAGGAGGTGT CCAGCCGTAC CGGCTCTTCC CTCAACCGGA TGGACCTCGT GGGTAAACGG
    GAGGCGTACG ACATGCACGA TACATTGCAT GTGCCTTATT CTTATGGAAT GCAGAGCAGA
    CACAGCCTGC CTGGGGAGTA CCAAGACTGC AATGGAAAGA CATCACCTGT TTTTCCTCCA
    CTCCATGTAG AAGAATCCAA GGTTGACTAT AGCAATAACA TCGATGATGA TGTAAACATG
    AGTAGGGGAG AAAGACTGAA AGCTTGGATC ATATCCCATT TACCAGCCTG GTGCAAGGAG
    AGAGAAACTT GGTCGCTGTA TTTGTTCCCG CCATATTCCA AATTCCGGCT GATGTGCAAT
    AAAATTATCA CCCACAGAAT GTTTGATCAC GTGGTCTTGG TCATCATATT TCTCAACTGT
    ATAACCATTG CCATGGAAAG GCCCAAAATA GACCCCCACA GTGCCGAAAG AATATTTCTC
    ACGTTGTCCA ACTACATATT CACCTTGATT TTCTTGGCTG AAATGACAGT GAAGGTGGTG
    GCCATGAACT TTTGCTTTGG TGGGAAGGCG TACCTGAGGA GCAGTTGGAA TGTGTTGGAT
    GGAATGCTGG TCTTGATCTC AGTCATTGAC ATTCTAGTCT CTATGGTGTC CGACAGCGGC
    ACCAAGATCC TTGGCATGCT AAGAGTGCTG CGGCTGCTCC GGACTTTAAG GCCACTCAGG
    GTGATCAGTC GAGCTCAGGG ACTGAAGCTG GTGGTTGAGA CACTAATGTC TTCTTTAAAG
    CCTATCGGTA ACATTGTGGT GATCTGCTGT GCCTTCTTCA TCATATTTGG GATACTTGGA
    GTTCAGCTTT TCAAGGGGAA ATTTTATTAC TGCCAGGGAG AGGACACTCG AAACATCACT
    AATAAATCCG ACTGCCTTGA AAACAAGTAT AAATGGGCAA GACACAAGTA CAACTTTGAT
    AATCTTGGCC AGGCTCTGAT GTCTCTGTTT GTCCTGGCAT CTAAAGACGG ATGGGTGGAC
    ATCATGTACG ATGGGCTGGA TGCTGTCGGG GTTGACCAGC AGCCAGTGAT GAACTACAAC
    CCTTGGATGC TGCTCTACTT CATCTCGTTT CTGCTGATCG TGGCGTTCTT TGTGCTCAAC
    ATGTTTGTGG GCGTGGTGGT GGAGAACTTC CATAAGTGCC GGCAGCATCA GGAGGAGGAG
    GAGGCGAAGA GACGGGAAGA GAAGAGGCTA CGCAGGATGG AGAAAAAGAG AAGGAATCTG
    ATGCTGGATG ATGTAATATT GGAGCACTCA GCCAGTCCAG TTAAAGAGGC CCAGTGCAAA
    CCCTACTACT CGGAGTATTC TCACCCTCGG CTGCTCATCC ATCAAATGTG CACAAGCCAT
    TATCTGGATT TGTTTATTAC TGGGGTCATC GGATTGAATG TTATCACTAT GGCGATGGAG
    CACTACCAGC AACCCAAGGT ATTGGATGAA GCTTTAAAAA TCTGTAATTA CATATTTACC
    CTCATTTTTG TGCTGGAATC TGGGTGCAAA ATGGTAGCTT TTGGCTTCCG GCGATTCTTC
    AAAGACAGGT GGAACCAGTT GGATTTGGCC ATTGTTCTGC TCTCCATTAT GGGGATCACC
    CTGGAGGAGA TTGAAGTCAA TGCATCGCTG CCCATTAACC CAACAATCAT CAGAATAATG
    AGGGTCCTGC GGATCGCTCG CGTGCTGAAG TTATTAAAGA TGGCAGTCGG TATGCGAGCC
    CTGCTGGATA CAGTATTGCA AGCGCTTCCT CAAGTGGGGA ATCTCGGCCT GCTCTTTATG
    CTGCTGTTTT TCATATTTGC CGCTTTGGGA GTTGAACTTT TTGGCGACTT AGAATGCACT
    GATTCCCACC CGTGTGAAGG TCTCGGCAGG CACGCCACGT TCAGGAACTT CGGCATGGCC
    TTTCTGACTT TGTTCAGAGT ATCCACAGGA GATAACTGGA ACGGTATAAT GAAGGACACA
    CTCCGTGATT GCAACGAGGA TTCCACCTGT TACAACACAG TCATCTCCCC CATCTATTTT
    GTCTCTTTTG TGCTGACTGC CCAGTTTGTC CTGGTCAATG TGGTAATAGC GGTGCTCATG
    AAACACCTTG AGGAAAGCAA CAAGGAAGCT AAGGAGGAGG CGGAGCTGGA GGCAGAGATG
    GAACTAGAGT TTCAGATCCA GAACATGCAG GTTCCGGATC CTGACCCATT TATGTGGGCT
    GGGAGAGATC TCGGCTATCC CCAGGAAGGT TCTTACGGAT ATATGAACTC CGTGCAGGAC
    AAGGCCGACT CACAGCTTTC ATTAGTCAAT CCCATGGAAA GACACATGTT TGATACAATA
    TCACTACTGA TCCAGGGATC TTTGGAAGGA GAGATGAAGC TGATGGACAA TTTGTCTGGC
    TCTGTGTGCC ACCATTACGC CCTCCCTCCC TCTGACAAGG GCTCCTCAGA AAAACAGATC
    CCATTGGCAG AGATGGAAGG CCTATCCATG ACATCAGACA TTCTGTCCGA GAAATCATGG
    TCCTTAGCTC TGACGGACGA CTCCCTGCCA GATGACCCTA ATCTCAACCC ATTTGGTGTA
    CTGGAAACCA AACTTGGCCT CCCCGAGGAG AAACATTCGG CAACCGTTGA GGAGGCTACG
    AATCTTCTCA GCGTCCGGAA ACCGTCAGTA TGCCGCACAC ATTCCCTGCC CAATGACAGC
    TACATGTTGC AAACGGGAAG CACGTGCGCT GAGTGCCAGG AGCAAAGAAA CCACTGTTAC
    CAGAAATCAG GTTCCAGCTC AACGGTTCAG TCTCAGCCAA ATGACCTCAA CTCTCTTCTC
    CAAGTGCCAC AAATCTCCAT CCCTTATGAA GCTTCAGGAA GTACAGTGAT ATCAGAGATT
    CCCAAACTCC CCGCCCCCTT GCGCTCTCCA TCGGCTAATA GGATGCTCCG CAGACAGGAA
    GCAATCAGAA CCGACTCTTT GGAATTTTTT CCCGATAGCA AAGAAAATGC TGGCATAACT
    GAGCCCCCAC CTGTATATAT TCCAATGTGC CAGGTGCCCA CCGAGACACC CTCGGCTTTT
    ATAGAACCAG AAAGAACTTT ATGCCAGTTG AAGCGACGCA GTGTTTACAC CCAGCAAGTC
    CAAGATACCT GCAAGATGTT TAGGTACCCT GATGCTTCTC AAACCAACAT GGACACGTTA
    CGAGAAACCT CAAAGATTCC AGAGAAAGAA GATTTGTTGA GCGCAGCATG TTGTAGGCGG
    CATCTCAACT CCAAAACGTG TAAAGCATTA AGCGTCTATT CTGCAGCATA CTCCTTAAGC
    CAGAAACTGA AACTAGGTAA CAGCGGCAAC GTTATTTTGA AGGACATGAA GAGGTTCTAC
    AGCGTAGATG CCCACGGCGT CCTTAAAAAG ACATCCTCTT GGTTGGATGA CCCCAGAAGA
    CATTCCATAG AAATTTGTTC TTTAGAGAAC ACTCTTCATC ACTACTCTAC TTCACGTTCT
    TGTGGATTCA TTAGCCACGT ATTCCAGAAG AAGGAGGACT TGCAAATAGT GCAGAGGACG
    GGCGGACAGG ATCCCGTCTG TCCAGATTGT GTTCACTCAG GAGAAGCATC AGAACTACCA
    CTCTCTTACG TCTCGAGTGG GCAATCACAA TCGACAGACT CGACCGAGCT CGCTAAAATA
    GACTCTTTCG TTCCGGAAAT GCAGACATCC CCTAAACCAC TGGGAGATTT AGTTTCTGTC
    GAAAGTTTTT CTATGGATGC AGTTGAATTA AATCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017953383.1
    CDS1664..8662
    Translation

    Target ORF information:

    RefSeq Version XM_018097894.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018097894.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGATAAAT CTCATGAGCA AAGACCTGCC AAAGACCCTG GGGATCCGGG GTCGGAACTG 
    GAAGGGCTGC CCTACCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ACGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTTCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGAGTCTTTC CAATGCTTCT
    TCAGCCACAC CCATGCCCAT GCCAAAATGT GCTTTGTTTG GAGATATCCC CATGGCTGGT
    GAATCTAGGA GGGAGAGTAA TGTCTCCGGT GGCCCCCATG CTTGTGAAAT CAGGTCTATG
    TCAAGTTCAA GGAACTCCCC GCACAGCTCC TGGGGTGCAG TGTGTAGTTG GAATAGCCGT
    AGATCTAGTT GGAACAGCAT TGGACGGGCT TCCAGCCTAA AACGGCACGA GCAAAGCGGT
    GAACACAAAT CTTTGCTGTC TGCTGATGGA AAAGAAAGCT CAGAAGGCGA CACATCAGAC
    GAGGAGGTGT CCAGCCGTAC CGGCTCTTCC CTCAACCGGA TGGACCTCGT GGGTAAACGG
    GAGGCGTACG ACATGCACGA TACATTGCAT GTGCCTTATT CTTATGGAAT GCAGAGCAGA
    CACAGCCTGC CTGGGGAGTA CCAAGACTGC AATGGAAAGA CATCACCTGT TTTTCCTCCA
    CTCCATGTAG AAGAATCCAA GGTTGACTAT AGCAATAACA TCGATGATGA TGTAAACATG
    AGTAGGGGAG AAAGACTGAA AGCTTGGATC ATATCCCATT TACCAGCCTG GTGCAAGGAG
    AGAGAAACTT GGTCGCTGTA TTTGTTCCCG CCATATTCCA AATTCCGGCT GATGTGCAAT
    AAAATTATCA CCCACAGAAT GTTTGATCAC GTGGTCTTGG TCATCATATT TCTCAACTGT
    ATAACCATTG CCATGGAAAG GCCCAAAATA GACCCCCACA GTGCCGAAAG AATATTTCTC
    ACGTTGTCCA ACTACATATT CACCTTGATT TTCTTGGCTG AAATGACAGT GAAGGTGGTG
    GCCATGAACT TTTGCTTTGG TGGGAAGGCG TACCTGAGGA GCAGTTGGAA TGTGTTGGAT
    GGAATGCTGG TCTTGATCTC AGTCATTGAC ATTCTAGTCT CTATGGTGTC CGACAGCGGC
    ACCAAGATCC TTGGCATGCT AAGAGTGCTG CGGCTGCTCC GGACTTTAAG GCCACTCAGG
    GTGATCAGTC GAGCTCAGGG ACTGAAGCTG GTGGTTGAGA CACTAATGTC TTCTTTAAAG
    CCTATCGGTA ACATTGTGGT GATCTGCTGT GCCTTCTTCA TCATATTTGG GATACTTGGA
    GTTCAGCTTT TCAAGGGGAA ATTTTATTAC TGCCAGGGAG AGGACACTCG AAACATCACT
    AATAAATCCG ACTGCCTTGA AAACAAGTAT AAATGGGCAA GACACAAGTA CAACTTTGAT
    AATCTTGGCC AGGCTCTGAT GTCTCTGTTT GTCCTGGCAT CTAAAGACGG ATGGGTGGAC
    ATCATGTACG ATGGGCTGGA TGCTGTCGGG GTTGACCAGC AGCCAGTGAT GAACTACAAC
    CCTTGGATGC TGCTCTACTT CATCTCGTTT CTGCTGATCG TGGCGTTCTT TGTGCTCAAC
    ATGTTTGTGG GCGTGGTGGT GGAGAACTTC CATAAGTGCC GGCAGCATCA GGAGGAGGAG
    GAGGCGAAGA GACGGGAAGA GAAGAGGCTA CGCAGGATGG AGAAAAAGAG AAGGAATCTG
    ATGCTGGATG ATGTAATATT GGAGCACTCA GCCAGTCCAG TTAAAGAGGC CCAGTGCAAA
    CCCTACTACT CGGAGTATTC TCACCCTCGG CTGCTCATCC ATCAAATGTG CACAAGCCAT
    TATCTGGATT TGTTTATTAC TGGGGTCATC GGATTGAATG TTATCACTAT GGCGATGGAG
    CACTACCAGC AACCCAAGGT ATTGGATGAA GCTTTAAAAA TCTGTAATTA CATATTTACC
    CTCATTTTTG TGCTGGAATC TGGGTGCAAA ATGGTAGCTT TTGGCTTCCG GCGATTCTTC
    AAAGACAGGT GGAACCAGTT GGATTTGGCC ATTGTTCTGC TCTCCATTAT GGGGATCACC
    CTGGAGGAGA TTGAAGTCAA TGCATCGCTG CCCATTAACC CAACAATCAT CAGAATAATG
    AGGGTCCTGC GGATCGCTCG CGTGCTGAAG TTATTAAAGA TGGCAGTCGG TATGCGAGCC
    CTGCTGGATA CAGTATTGCA AGCGCTTCCT CAAGTGGGGA ATCTCGGCCT GCTCTTTATG
    CTGCTGTTTT TCATATTTGC CGCTTTGGGA GTTGAACTTT TTGGCGACTT AGAATGCACT
    GATTCCCACC CGTGTGAAGG TCTCGGCAGG CACGCCACGT TCAGGAACTT CGGCATGGCC
    TTTCTGACTT TGTTCAGAGT ATCCACAGGA GATAACTGGA ACGGTATAAT GAAGGACACA
    CTCCGTGATT GCAACGAGGA TTCCACCTGT TACAACACAG TCATCTCCCC CATCTATTTT
    GTCTCTTTTG TGCTGACTGC CCAGTTTGTC CTGGTCAATG TGGTAATAGC GGTGCTCATG
    AAACACCTTG AGGAAAGCAA CAAGGAAGCT AAGGAGGAGG CGGAGCTGGA GGCAGAGATG
    GAACTAGAGT TTCAGATCCA GAACATGCAG GTTCCGGATC CTGACCCATT TATGTGGGCT
    GGGAGAGATC TCGGCTATCC CCAGGAAGGT TCTTACGGAT ATATGAACTC CGTGCAGGAC
    AAGGCCGACT CACAGCTTTC ATTAGTCAAT CCCATGGAAA GACACATGTT TGATACAATA
    TCACTACTGA TCCAGGGATC TTTGGAAGGA GAGATGAAGC TGATGGACAA TTTGTCTGGC
    TCTGTGTGCC ACCATTACGC CCTCCCTCCC TCTGACAAGG GCTCCTCAGA AAAACAGATC
    CCATTGGCAG AGATGGAAGG CCTATCCATG ACATCAGACA TTCTGTCCGA GAAATCATGG
    TCCTTAGCTC TGACGGACGA CTCCCTGCCA GATGACCCTA ATCTCAACCC ATTTGGTGTA
    CTGGAAACCA AACTTGGCCT CCCCGAGGAG AAACATTCGG CAACCGTTGA GGAGGCTACG
    AATCTTCTCA GCGTCCGGAA ACCGTCAGTA TGCCGCACAC ATTCCCTGCC CAATGACAGC
    TACATGTTGC AAACGGGAAG CACGTGCGCT GAGTGCCAGG AGCAAAGAAA CCACTGTTAC
    CAGAAATCAG GTTCCAGCTC AACGGTTCAG TCTCAGCCAA ATGACCTCAA CTCTCTTCTC
    CAAGTGCCAC AAATCTCCAT CCCTTATGAA GCTTCAGGAA GTACAGTGAT ATCAGAGATT
    CCCAAACTCC CCGCCCCCTT GCGCTCTCCA TCGGCTAATA GGATGCTCCG CAGACAGGAA
    GCAATCAGAA CCGACTCTTT GGAATTTTTT CCCGATAGCA AAGAAAATGC TGGCATAACT
    GAGCCCCCAC CTGTATATAT TCCAATGTGC CAGGTGCCCA CCGAGACACC CTCGGCTTTT
    ATAGAACCAG AAAGAACTTT ATGCCAGTTG AAGCGACGCA GTGTTTACAC CCAGCAAGTC
    CAAGATACCT GCAAGATGTT TAGGTACCCT GATGCTTCTC AAACCAACAT GGACACGTTA
    CGAGAAACCT CAAAGATTCC AGAGAAAGAA GATTTGTTGA GCGCAGCATG TTGTAGGCGG
    CATCTCAACT CCAAAACGTG TAAAGCATTA AGCGTCTATT CTGCAGCATA CTCCTTAAGC
    CAGAAACTGA AACTAGGTAA CAGCGGCAAC GTTATTTTGA AGGACATGAA GAGGTTCTAC
    AGCGTAGATG CCCACGGCGT CCTTAAAAAG ACATCCTCTT GGTTGGATGA CCCCAGAAGA
    CATTCCATAG AAATTTGTTC TTTAGAGAAC ACTCTTCATC ACTACTCTAC TTCACGTTCT
    TGTGGATTCA TTAGCCACGT ATTCCAGAAG AAGGAGGACT TGCAAATAGT GCAGAGGACG
    GGCGGACAGG ATCCCGTCTG TCCAGATTGT GTTCACTCAG GAGAAGCATC AGAACTACCA
    CTCTCTTACG TCTCGAGTGG GCAATCACAA TCGACAGACT CGACCGAGCT CGCTAAAATA
    GACTCTTTCG TTCCGGAAAT GCAGACATCC CCTAAACCAC TGGGAGATTT AGTTTCTGTC
    GAAAGTTTTT CTATGGATGC AGTTGAATTA AATCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51011
    Clone ID Related Accession (Same CDS sequence) XM_031894865.1 , XM_031894866.1 , XM_031894867.1 , XM_031894869.1 , XM_031894868.1 , XM_031894870.1
    Accession Version XM_031894865.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7086bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATCGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCCTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AGTAAGGAGA GAGAGCTGGC CGATCTGATG CTGGATGATG TAATATTGGA GCACTCAGCC
    AGTCCAGTTA AAGAGGCCCA GTGCAAACCC TACTACTCGG AGTATTCTCA CCCTCGGCTG
    CTCATCCATC AAATGTGCAC AAGCCATTAT CTGGATTTGT TTATTACTGG GGTCATCGGA
    TTGAATGTTA TCACTATGGC GATGGAGCAC TACCAGCAAC CCAAGGTATT GGATGAAGCT
    CTAAAAATCT GTAATTACAT ATTTACCCTC ATTTTTGTGC TGGAATCTGG GTGCAAAATG
    GTAGCTTTTG GCTTCCGGCG ATTCTTCAAA GACAGGTGGA ACCAGTTGGA TTTGGCCATT
    GTTCTGCTCT CCATTATGGG GATCACCCTG GAGGAGATTG AAGTCAATGC ATCGCTGCCC
    ATTAACCCAA CAATCATCAG AATAATGAGG GTCCTGCGGA TCGCTCGCGT GCTGAAGTTA
    TTAAAGATGG CAGTCGGTAT GCGAGCCCTG CTGGATACAG TATTGCAAGC GCTTCCTCAA
    GTGGGGAATC TCGGCCTGCT CTTTATGCTG CTGTTTTTCA TATTTGCCGC TTTGGGAGTT
    GAACTTTTTG GCGACTTAGA ATGCACTGAT TCCCACCCGT GTGAAGGTCT CGGCAGGCAC
    GCCACGTTCA GGAACTTCGG CATGGCCTTT CTGACTTTGT TCAGAGTATC CACAGGAGAT
    AACTGGAACG GTATAATGAA GGACACACTC CGTGATTGCA ACGAGGATTC CACCTGTTAC
    AACACAGTCA TCTCCCCCAT CTATTTTGTC TCTTTTGTGC TGACTGCCCA GTTTGTCCTG
    GTCAATGTGG TAATAGCGGT GCTCATGAAA CACCTTGAGG AAAGCAACAA GGAAGCTAAG
    GAGGAGGCGG AGCTGGAGGC AGAGATGGAA CTAGAGTTTC AGATCCAGAA CATGCAGGTT
    CCGGATCCTG ACCCATTTAT GTGGGCTGGG AGAGATCTCG GCTATCCCCA GGAAGGTTCT
    TACGGATATA TGAACTCCGT GCAGGACAAG GCCGACTCAC AGCTTTCATT AGTCAATCCC
    ATGGAAAGAC ACATGTTTGA TACAATATCA CTACTGATCC AGGGATCTTT GGAAGGAGAG
    ATGAAGCTGA TGGACAATTT GTCTGGCTCT GTGTGCCACC ATTACGCCCT CCCTCCCTCT
    GACAAGGGCT CCTCGGAAAA ACAGATCCCA TTGGCAGAGA TGGAAGGCCT ATCCATGACA
    TCAGACATTC TGTCCGAGAA ATCATGGTCC TTAGCTCTGA CGGACGACTC CCTGCCAGAT
    GACCCTAATC CCAACCCATT TGGTGTACTG GAAACCAAAC TTGGCCTCCC CGAGGAGAAA
    CATTCGGCAA CCGTTGAGGA GGCTACGAAT CTTCTCAGCG TCCGGAAACC GTCAGTATGC
    CGCACACATT CCCTGCCCAA TGACAGCTAC ATGTTGCAAA CGGGAAGCAC GTGCACTGAG
    TGCCAGGAGC AAAGAAACCA CTGTTACCAG AAATCAGGTT CCAGCTCAAC GGTTCAGTCT
    CAGCCAAATG ACCTCAACTC TCTTCTCCAA GTGCCACAAA TCTCCATCCC TTATGAAGCT
    TCAGGAAGTA CAGTGATATC AGAGATTCCC AAACTCCCCG CCCCCTTGCG CTCTCCATCG
    GCTAATAGGA TGCTCCGCAG ACAGGAAGCA ATCAGAACCG ACTCTTTGGA ATTTTTTCCC
    GATAGCAAAG AAAATGCTGG CATAACTGAG CCCCCACCTG TATATATTCC AATGTGCCAG
    GTGCCCACCG AGACACCCTC GGCTTTTATA GAACCAGAAA GAACTTTATG CCAGTTGAAG
    CGACGCAGTG TTTACACCCA GCAAGTCCAA GATACCTGCA AGATGTTTAG GTACCCTGAT
    GCTTCTCAAA CCAACATGGA CACGTTACGA GAAACCTCAA AGATTCCAGA GAAAGAAGAT
    TTGTTGAGCG CAGCATGTTG TAGGCGGCAT CTCAACTCCA AAACGTGTAA AGCATTAAGC
    GTCTATTCTG CAGCATACTC CTTAAGCCAG AAACTGAAAC TAGGTAACAG CGGCAACGTT
    ATTTTGAAGG ACATGAAGAG GTTCTACAGC GTAGATGCCC ACGGCGTCCT TAAAAAGACA
    TCCTCTTGGT TGGATGACCC CAGAAGACAT TCCATAGAAA TTTGTTCTTT AGAGAACACT
    CTTCATCACT ACTCTACTTC ACGTTCTTGT GGATTCATTA GCCACGTATT CCAGAAGAAG
    GAGGACTTGC AAATAGTGCA GAGGACGGGC GGACAGGATC CCGTCTGTCC AGATTGTGTT
    CACTCAGGAG AAGCATCAGA ACTACCACTC TCTTACGTCT CGAGTGGGCA ATCACAATCG
    ACAGACTCGA CCGAGCTCGG TAAAATAGAC TCTTTCGTTC CGGAAATGCA GACATCCCCT
    AAACCACTGG GAGATTTAGT TTCTGTCGAA AGTTTTTCTA TGGATGCAGT TGAATTAAAT
    CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750725.1
    CDS1990..9075
    Translation

    Target ORF information:

    RefSeq Version XM_031894865.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894865.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATCGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCCTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AGTAAGGAGA GAGAGCTGGC CGATCTGATG CTGGATGATG TAATATTGGA GCACTCAGCC
    AGTCCAGTTA AAGAGGCCCA GTGCAAACCC TACTACTCGG AGTATTCTCA CCCTCGGCTG
    CTCATCCATC AAATGTGCAC AAGCCATTAT CTGGATTTGT TTATTACTGG GGTCATCGGA
    TTGAATGTTA TCACTATGGC GATGGAGCAC TACCAGCAAC CCAAGGTATT GGATGAAGCT
    CTAAAAATCT GTAATTACAT ATTTACCCTC ATTTTTGTGC TGGAATCTGG GTGCAAAATG
    GTAGCTTTTG GCTTCCGGCG ATTCTTCAAA GACAGGTGGA ACCAGTTGGA TTTGGCCATT
    GTTCTGCTCT CCATTATGGG GATCACCCTG GAGGAGATTG AAGTCAATGC ATCGCTGCCC
    ATTAACCCAA CAATCATCAG AATAATGAGG GTCCTGCGGA TCGCTCGCGT GCTGAAGTTA
    TTAAAGATGG CAGTCGGTAT GCGAGCCCTG CTGGATACAG TATTGCAAGC GCTTCCTCAA
    GTGGGGAATC TCGGCCTGCT CTTTATGCTG CTGTTTTTCA TATTTGCCGC TTTGGGAGTT
    GAACTTTTTG GCGACTTAGA ATGCACTGAT TCCCACCCGT GTGAAGGTCT CGGCAGGCAC
    GCCACGTTCA GGAACTTCGG CATGGCCTTT CTGACTTTGT TCAGAGTATC CACAGGAGAT
    AACTGGAACG GTATAATGAA GGACACACTC CGTGATTGCA ACGAGGATTC CACCTGTTAC
    AACACAGTCA TCTCCCCCAT CTATTTTGTC TCTTTTGTGC TGACTGCCCA GTTTGTCCTG
    GTCAATGTGG TAATAGCGGT GCTCATGAAA CACCTTGAGG AAAGCAACAA GGAAGCTAAG
    GAGGAGGCGG AGCTGGAGGC AGAGATGGAA CTAGAGTTTC AGATCCAGAA CATGCAGGTT
    CCGGATCCTG ACCCATTTAT GTGGGCTGGG AGAGATCTCG GCTATCCCCA GGAAGGTTCT
    TACGGATATA TGAACTCCGT GCAGGACAAG GCCGACTCAC AGCTTTCATT AGTCAATCCC
    ATGGAAAGAC ACATGTTTGA TACAATATCA CTACTGATCC AGGGATCTTT GGAAGGAGAG
    ATGAAGCTGA TGGACAATTT GTCTGGCTCT GTGTGCCACC ATTACGCCCT CCCTCCCTCT
    GACAAGGGCT CCTCGGAAAA ACAGATCCCA TTGGCAGAGA TGGAAGGCCT ATCCATGACA
    TCAGACATTC TGTCCGAGAA ATCATGGTCC TTAGCTCTGA CGGACGACTC CCTGCCAGAT
    GACCCTAATC CCAACCCATT TGGTGTACTG GAAACCAAAC TTGGCCTCCC CGAGGAGAAA
    CATTCGGCAA CCGTTGAGGA GGCTACGAAT CTTCTCAGCG TCCGGAAACC GTCAGTATGC
    CGCACACATT CCCTGCCCAA TGACAGCTAC ATGTTGCAAA CGGGAAGCAC GTGCACTGAG
    TGCCAGGAGC AAAGAAACCA CTGTTACCAG AAATCAGGTT CCAGCTCAAC GGTTCAGTCT
    CAGCCAAATG ACCTCAACTC TCTTCTCCAA GTGCCACAAA TCTCCATCCC TTATGAAGCT
    TCAGGAAGTA CAGTGATATC AGAGATTCCC AAACTCCCCG CCCCCTTGCG CTCTCCATCG
    GCTAATAGGA TGCTCCGCAG ACAGGAAGCA ATCAGAACCG ACTCTTTGGA ATTTTTTCCC
    GATAGCAAAG AAAATGCTGG CATAACTGAG CCCCCACCTG TATATATTCC AATGTGCCAG
    GTGCCCACCG AGACACCCTC GGCTTTTATA GAACCAGAAA GAACTTTATG CCAGTTGAAG
    CGACGCAGTG TTTACACCCA GCAAGTCCAA GATACCTGCA AGATGTTTAG GTACCCTGAT
    GCTTCTCAAA CCAACATGGA CACGTTACGA GAAACCTCAA AGATTCCAGA GAAAGAAGAT
    TTGTTGAGCG CAGCATGTTG TAGGCGGCAT CTCAACTCCA AAACGTGTAA AGCATTAAGC
    GTCTATTCTG CAGCATACTC CTTAAGCCAG AAACTGAAAC TAGGTAACAG CGGCAACGTT
    ATTTTGAAGG ACATGAAGAG GTTCTACAGC GTAGATGCCC ACGGCGTCCT TAAAAAGACA
    TCCTCTTGGT TGGATGACCC CAGAAGACAT TCCATAGAAA TTTGTTCTTT AGAGAACACT
    CTTCATCACT ACTCTACTTC ACGTTCTTGT GGATTCATTA GCCACGTATT CCAGAAGAAG
    GAGGACTTGC AAATAGTGCA GAGGACGGGC GGACAGGATC CCGTCTGTCC AGATTGTGTT
    CACTCAGGAG AAGCATCAGA ACTACCACTC TCTTACGTCT CGAGTGGGCA ATCACAATCG
    ACAGACTCGA CCGAGCTCGG TAAAATAGAC TCTTTCGTTC CGGAAATGCA GACATCCCCT
    AAACCACTGG GAGATTTAGT TTCTGTCGAA AGTTTTTCTA TGGATGCAGT TGAATTAAAT
    CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51011
    Clone ID Related Accession (Same CDS sequence) XM_031894865.1 , XM_031894866.1 , XM_031894867.1 , XM_031894869.1 , XM_031894868.1 , XM_031894870.1
    Accession Version XM_031894866.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7086bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATCGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCCTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AGTAAGGAGA GAGAGCTGGC CGATCTGATG CTGGATGATG TAATATTGGA GCACTCAGCC
    AGTCCAGTTA AAGAGGCCCA GTGCAAACCC TACTACTCGG AGTATTCTCA CCCTCGGCTG
    CTCATCCATC AAATGTGCAC AAGCCATTAT CTGGATTTGT TTATTACTGG GGTCATCGGA
    TTGAATGTTA TCACTATGGC GATGGAGCAC TACCAGCAAC CCAAGGTATT GGATGAAGCT
    CTAAAAATCT GTAATTACAT ATTTACCCTC ATTTTTGTGC TGGAATCTGG GTGCAAAATG
    GTAGCTTTTG GCTTCCGGCG ATTCTTCAAA GACAGGTGGA ACCAGTTGGA TTTGGCCATT
    GTTCTGCTCT CCATTATGGG GATCACCCTG GAGGAGATTG AAGTCAATGC ATCGCTGCCC
    ATTAACCCAA CAATCATCAG AATAATGAGG GTCCTGCGGA TCGCTCGCGT GCTGAAGTTA
    TTAAAGATGG CAGTCGGTAT GCGAGCCCTG CTGGATACAG TATTGCAAGC GCTTCCTCAA
    GTGGGGAATC TCGGCCTGCT CTTTATGCTG CTGTTTTTCA TATTTGCCGC TTTGGGAGTT
    GAACTTTTTG GCGACTTAGA ATGCACTGAT TCCCACCCGT GTGAAGGTCT CGGCAGGCAC
    GCCACGTTCA GGAACTTCGG CATGGCCTTT CTGACTTTGT TCAGAGTATC CACAGGAGAT
    AACTGGAACG GTATAATGAA GGACACACTC CGTGATTGCA ACGAGGATTC CACCTGTTAC
    AACACAGTCA TCTCCCCCAT CTATTTTGTC TCTTTTGTGC TGACTGCCCA GTTTGTCCTG
    GTCAATGTGG TAATAGCGGT GCTCATGAAA CACCTTGAGG AAAGCAACAA GGAAGCTAAG
    GAGGAGGCGG AGCTGGAGGC AGAGATGGAA CTAGAGTTTC AGATCCAGAA CATGCAGGTT
    CCGGATCCTG ACCCATTTAT GTGGGCTGGG AGAGATCTCG GCTATCCCCA GGAAGGTTCT
    TACGGATATA TGAACTCCGT GCAGGACAAG GCCGACTCAC AGCTTTCATT AGTCAATCCC
    ATGGAAAGAC ACATGTTTGA TACAATATCA CTACTGATCC AGGGATCTTT GGAAGGAGAG
    ATGAAGCTGA TGGACAATTT GTCTGGCTCT GTGTGCCACC ATTACGCCCT CCCTCCCTCT
    GACAAGGGCT CCTCGGAAAA ACAGATCCCA TTGGCAGAGA TGGAAGGCCT ATCCATGACA
    TCAGACATTC TGTCCGAGAA ATCATGGTCC TTAGCTCTGA CGGACGACTC CCTGCCAGAT
    GACCCTAATC CCAACCCATT TGGTGTACTG GAAACCAAAC TTGGCCTCCC CGAGGAGAAA
    CATTCGGCAA CCGTTGAGGA GGCTACGAAT CTTCTCAGCG TCCGGAAACC GTCAGTATGC
    CGCACACATT CCCTGCCCAA TGACAGCTAC ATGTTGCAAA CGGGAAGCAC GTGCACTGAG
    TGCCAGGAGC AAAGAAACCA CTGTTACCAG AAATCAGGTT CCAGCTCAAC GGTTCAGTCT
    CAGCCAAATG ACCTCAACTC TCTTCTCCAA GTGCCACAAA TCTCCATCCC TTATGAAGCT
    TCAGGAAGTA CAGTGATATC AGAGATTCCC AAACTCCCCG CCCCCTTGCG CTCTCCATCG
    GCTAATAGGA TGCTCCGCAG ACAGGAAGCA ATCAGAACCG ACTCTTTGGA ATTTTTTCCC
    GATAGCAAAG AAAATGCTGG CATAACTGAG CCCCCACCTG TATATATTCC AATGTGCCAG
    GTGCCCACCG AGACACCCTC GGCTTTTATA GAACCAGAAA GAACTTTATG CCAGTTGAAG
    CGACGCAGTG TTTACACCCA GCAAGTCCAA GATACCTGCA AGATGTTTAG GTACCCTGAT
    GCTTCTCAAA CCAACATGGA CACGTTACGA GAAACCTCAA AGATTCCAGA GAAAGAAGAT
    TTGTTGAGCG CAGCATGTTG TAGGCGGCAT CTCAACTCCA AAACGTGTAA AGCATTAAGC
    GTCTATTCTG CAGCATACTC CTTAAGCCAG AAACTGAAAC TAGGTAACAG CGGCAACGTT
    ATTTTGAAGG ACATGAAGAG GTTCTACAGC GTAGATGCCC ACGGCGTCCT TAAAAAGACA
    TCCTCTTGGT TGGATGACCC CAGAAGACAT TCCATAGAAA TTTGTTCTTT AGAGAACACT
    CTTCATCACT ACTCTACTTC ACGTTCTTGT GGATTCATTA GCCACGTATT CCAGAAGAAG
    GAGGACTTGC AAATAGTGCA GAGGACGGGC GGACAGGATC CCGTCTGTCC AGATTGTGTT
    CACTCAGGAG AAGCATCAGA ACTACCACTC TCTTACGTCT CGAGTGGGCA ATCACAATCG
    ACAGACTCGA CCGAGCTCGG TAAAATAGAC TCTTTCGTTC CGGAAATGCA GACATCCCCT
    AAACCACTGG GAGATTTAGT TTCTGTCGAA AGTTTTTCTA TGGATGCAGT TGAATTAAAT
    CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750726.1
    CDS1948..9033
    Translation

    Target ORF information:

    RefSeq Version XM_031894866.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894866.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATCGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCCTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AGTAAGGAGA GAGAGCTGGC CGATCTGATG CTGGATGATG TAATATTGGA GCACTCAGCC
    AGTCCAGTTA AAGAGGCCCA GTGCAAACCC TACTACTCGG AGTATTCTCA CCCTCGGCTG
    CTCATCCATC AAATGTGCAC AAGCCATTAT CTGGATTTGT TTATTACTGG GGTCATCGGA
    TTGAATGTTA TCACTATGGC GATGGAGCAC TACCAGCAAC CCAAGGTATT GGATGAAGCT
    CTAAAAATCT GTAATTACAT ATTTACCCTC ATTTTTGTGC TGGAATCTGG GTGCAAAATG
    GTAGCTTTTG GCTTCCGGCG ATTCTTCAAA GACAGGTGGA ACCAGTTGGA TTTGGCCATT
    GTTCTGCTCT CCATTATGGG GATCACCCTG GAGGAGATTG AAGTCAATGC ATCGCTGCCC
    ATTAACCCAA CAATCATCAG AATAATGAGG GTCCTGCGGA TCGCTCGCGT GCTGAAGTTA
    TTAAAGATGG CAGTCGGTAT GCGAGCCCTG CTGGATACAG TATTGCAAGC GCTTCCTCAA
    GTGGGGAATC TCGGCCTGCT CTTTATGCTG CTGTTTTTCA TATTTGCCGC TTTGGGAGTT
    GAACTTTTTG GCGACTTAGA ATGCACTGAT TCCCACCCGT GTGAAGGTCT CGGCAGGCAC
    GCCACGTTCA GGAACTTCGG CATGGCCTTT CTGACTTTGT TCAGAGTATC CACAGGAGAT
    AACTGGAACG GTATAATGAA GGACACACTC CGTGATTGCA ACGAGGATTC CACCTGTTAC
    AACACAGTCA TCTCCCCCAT CTATTTTGTC TCTTTTGTGC TGACTGCCCA GTTTGTCCTG
    GTCAATGTGG TAATAGCGGT GCTCATGAAA CACCTTGAGG AAAGCAACAA GGAAGCTAAG
    GAGGAGGCGG AGCTGGAGGC AGAGATGGAA CTAGAGTTTC AGATCCAGAA CATGCAGGTT
    CCGGATCCTG ACCCATTTAT GTGGGCTGGG AGAGATCTCG GCTATCCCCA GGAAGGTTCT
    TACGGATATA TGAACTCCGT GCAGGACAAG GCCGACTCAC AGCTTTCATT AGTCAATCCC
    ATGGAAAGAC ACATGTTTGA TACAATATCA CTACTGATCC AGGGATCTTT GGAAGGAGAG
    ATGAAGCTGA TGGACAATTT GTCTGGCTCT GTGTGCCACC ATTACGCCCT CCCTCCCTCT
    GACAAGGGCT CCTCGGAAAA ACAGATCCCA TTGGCAGAGA TGGAAGGCCT ATCCATGACA
    TCAGACATTC TGTCCGAGAA ATCATGGTCC TTAGCTCTGA CGGACGACTC CCTGCCAGAT
    GACCCTAATC CCAACCCATT TGGTGTACTG GAAACCAAAC TTGGCCTCCC CGAGGAGAAA
    CATTCGGCAA CCGTTGAGGA GGCTACGAAT CTTCTCAGCG TCCGGAAACC GTCAGTATGC
    CGCACACATT CCCTGCCCAA TGACAGCTAC ATGTTGCAAA CGGGAAGCAC GTGCACTGAG
    TGCCAGGAGC AAAGAAACCA CTGTTACCAG AAATCAGGTT CCAGCTCAAC GGTTCAGTCT
    CAGCCAAATG ACCTCAACTC TCTTCTCCAA GTGCCACAAA TCTCCATCCC TTATGAAGCT
    TCAGGAAGTA CAGTGATATC AGAGATTCCC AAACTCCCCG CCCCCTTGCG CTCTCCATCG
    GCTAATAGGA TGCTCCGCAG ACAGGAAGCA ATCAGAACCG ACTCTTTGGA ATTTTTTCCC
    GATAGCAAAG AAAATGCTGG CATAACTGAG CCCCCACCTG TATATATTCC AATGTGCCAG
    GTGCCCACCG AGACACCCTC GGCTTTTATA GAACCAGAAA GAACTTTATG CCAGTTGAAG
    CGACGCAGTG TTTACACCCA GCAAGTCCAA GATACCTGCA AGATGTTTAG GTACCCTGAT
    GCTTCTCAAA CCAACATGGA CACGTTACGA GAAACCTCAA AGATTCCAGA GAAAGAAGAT
    TTGTTGAGCG CAGCATGTTG TAGGCGGCAT CTCAACTCCA AAACGTGTAA AGCATTAAGC
    GTCTATTCTG CAGCATACTC CTTAAGCCAG AAACTGAAAC TAGGTAACAG CGGCAACGTT
    ATTTTGAAGG ACATGAAGAG GTTCTACAGC GTAGATGCCC ACGGCGTCCT TAAAAAGACA
    TCCTCTTGGT TGGATGACCC CAGAAGACAT TCCATAGAAA TTTGTTCTTT AGAGAACACT
    CTTCATCACT ACTCTACTTC ACGTTCTTGT GGATTCATTA GCCACGTATT CCAGAAGAAG
    GAGGACTTGC AAATAGTGCA GAGGACGGGC GGACAGGATC CCGTCTGTCC AGATTGTGTT
    CACTCAGGAG AAGCATCAGA ACTACCACTC TCTTACGTCT CGAGTGGGCA ATCACAATCG
    ACAGACTCGA CCGAGCTCGG TAAAATAGAC TCTTTCGTTC CGGAAATGCA GACATCCCCT
    AAACCACTGG GAGATTTAGT TTCTGTCGAA AGTTTTTCTA TGGATGCAGT TGAATTAAAT
    CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51011
    Clone ID Related Accession (Same CDS sequence) XM_031894865.1 , XM_031894866.1 , XM_031894867.1 , XM_031894869.1 , XM_031894868.1 , XM_031894870.1
    Accession Version XM_031894867.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7086bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATCGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCCTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AGTAAGGAGA GAGAGCTGGC CGATCTGATG CTGGATGATG TAATATTGGA GCACTCAGCC
    AGTCCAGTTA AAGAGGCCCA GTGCAAACCC TACTACTCGG AGTATTCTCA CCCTCGGCTG
    CTCATCCATC AAATGTGCAC AAGCCATTAT CTGGATTTGT TTATTACTGG GGTCATCGGA
    TTGAATGTTA TCACTATGGC GATGGAGCAC TACCAGCAAC CCAAGGTATT GGATGAAGCT
    CTAAAAATCT GTAATTACAT ATTTACCCTC ATTTTTGTGC TGGAATCTGG GTGCAAAATG
    GTAGCTTTTG GCTTCCGGCG ATTCTTCAAA GACAGGTGGA ACCAGTTGGA TTTGGCCATT
    GTTCTGCTCT CCATTATGGG GATCACCCTG GAGGAGATTG AAGTCAATGC ATCGCTGCCC
    ATTAACCCAA CAATCATCAG AATAATGAGG GTCCTGCGGA TCGCTCGCGT GCTGAAGTTA
    TTAAAGATGG CAGTCGGTAT GCGAGCCCTG CTGGATACAG TATTGCAAGC GCTTCCTCAA
    GTGGGGAATC TCGGCCTGCT CTTTATGCTG CTGTTTTTCA TATTTGCCGC TTTGGGAGTT
    GAACTTTTTG GCGACTTAGA ATGCACTGAT TCCCACCCGT GTGAAGGTCT CGGCAGGCAC
    GCCACGTTCA GGAACTTCGG CATGGCCTTT CTGACTTTGT TCAGAGTATC CACAGGAGAT
    AACTGGAACG GTATAATGAA GGACACACTC CGTGATTGCA ACGAGGATTC CACCTGTTAC
    AACACAGTCA TCTCCCCCAT CTATTTTGTC TCTTTTGTGC TGACTGCCCA GTTTGTCCTG
    GTCAATGTGG TAATAGCGGT GCTCATGAAA CACCTTGAGG AAAGCAACAA GGAAGCTAAG
    GAGGAGGCGG AGCTGGAGGC AGAGATGGAA CTAGAGTTTC AGATCCAGAA CATGCAGGTT
    CCGGATCCTG ACCCATTTAT GTGGGCTGGG AGAGATCTCG GCTATCCCCA GGAAGGTTCT
    TACGGATATA TGAACTCCGT GCAGGACAAG GCCGACTCAC AGCTTTCATT AGTCAATCCC
    ATGGAAAGAC ACATGTTTGA TACAATATCA CTACTGATCC AGGGATCTTT GGAAGGAGAG
    ATGAAGCTGA TGGACAATTT GTCTGGCTCT GTGTGCCACC ATTACGCCCT CCCTCCCTCT
    GACAAGGGCT CCTCGGAAAA ACAGATCCCA TTGGCAGAGA TGGAAGGCCT ATCCATGACA
    TCAGACATTC TGTCCGAGAA ATCATGGTCC TTAGCTCTGA CGGACGACTC CCTGCCAGAT
    GACCCTAATC CCAACCCATT TGGTGTACTG GAAACCAAAC TTGGCCTCCC CGAGGAGAAA
    CATTCGGCAA CCGTTGAGGA GGCTACGAAT CTTCTCAGCG TCCGGAAACC GTCAGTATGC
    CGCACACATT CCCTGCCCAA TGACAGCTAC ATGTTGCAAA CGGGAAGCAC GTGCACTGAG
    TGCCAGGAGC AAAGAAACCA CTGTTACCAG AAATCAGGTT CCAGCTCAAC GGTTCAGTCT
    CAGCCAAATG ACCTCAACTC TCTTCTCCAA GTGCCACAAA TCTCCATCCC TTATGAAGCT
    TCAGGAAGTA CAGTGATATC AGAGATTCCC AAACTCCCCG CCCCCTTGCG CTCTCCATCG
    GCTAATAGGA TGCTCCGCAG ACAGGAAGCA ATCAGAACCG ACTCTTTGGA ATTTTTTCCC
    GATAGCAAAG AAAATGCTGG CATAACTGAG CCCCCACCTG TATATATTCC AATGTGCCAG
    GTGCCCACCG AGACACCCTC GGCTTTTATA GAACCAGAAA GAACTTTATG CCAGTTGAAG
    CGACGCAGTG TTTACACCCA GCAAGTCCAA GATACCTGCA AGATGTTTAG GTACCCTGAT
    GCTTCTCAAA CCAACATGGA CACGTTACGA GAAACCTCAA AGATTCCAGA GAAAGAAGAT
    TTGTTGAGCG CAGCATGTTG TAGGCGGCAT CTCAACTCCA AAACGTGTAA AGCATTAAGC
    GTCTATTCTG CAGCATACTC CTTAAGCCAG AAACTGAAAC TAGGTAACAG CGGCAACGTT
    ATTTTGAAGG ACATGAAGAG GTTCTACAGC GTAGATGCCC ACGGCGTCCT TAAAAAGACA
    TCCTCTTGGT TGGATGACCC CAGAAGACAT TCCATAGAAA TTTGTTCTTT AGAGAACACT
    CTTCATCACT ACTCTACTTC ACGTTCTTGT GGATTCATTA GCCACGTATT CCAGAAGAAG
    GAGGACTTGC AAATAGTGCA GAGGACGGGC GGACAGGATC CCGTCTGTCC AGATTGTGTT
    CACTCAGGAG AAGCATCAGA ACTACCACTC TCTTACGTCT CGAGTGGGCA ATCACAATCG
    ACAGACTCGA CCGAGCTCGG TAAAATAGAC TCTTTCGTTC CGGAAATGCA GACATCCCCT
    AAACCACTGG GAGATTTAGT TTCTGTCGAA AGTTTTTCTA TGGATGCAGT TGAATTAAAT
    CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750727.1
    CDS1994..9079
    Translation

    Target ORF information:

    RefSeq Version XM_031894867.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894867.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATCGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCCTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AGTAAGGAGA GAGAGCTGGC CGATCTGATG CTGGATGATG TAATATTGGA GCACTCAGCC
    AGTCCAGTTA AAGAGGCCCA GTGCAAACCC TACTACTCGG AGTATTCTCA CCCTCGGCTG
    CTCATCCATC AAATGTGCAC AAGCCATTAT CTGGATTTGT TTATTACTGG GGTCATCGGA
    TTGAATGTTA TCACTATGGC GATGGAGCAC TACCAGCAAC CCAAGGTATT GGATGAAGCT
    CTAAAAATCT GTAATTACAT ATTTACCCTC ATTTTTGTGC TGGAATCTGG GTGCAAAATG
    GTAGCTTTTG GCTTCCGGCG ATTCTTCAAA GACAGGTGGA ACCAGTTGGA TTTGGCCATT
    GTTCTGCTCT CCATTATGGG GATCACCCTG GAGGAGATTG AAGTCAATGC ATCGCTGCCC
    ATTAACCCAA CAATCATCAG AATAATGAGG GTCCTGCGGA TCGCTCGCGT GCTGAAGTTA
    TTAAAGATGG CAGTCGGTAT GCGAGCCCTG CTGGATACAG TATTGCAAGC GCTTCCTCAA
    GTGGGGAATC TCGGCCTGCT CTTTATGCTG CTGTTTTTCA TATTTGCCGC TTTGGGAGTT
    GAACTTTTTG GCGACTTAGA ATGCACTGAT TCCCACCCGT GTGAAGGTCT CGGCAGGCAC
    GCCACGTTCA GGAACTTCGG CATGGCCTTT CTGACTTTGT TCAGAGTATC CACAGGAGAT
    AACTGGAACG GTATAATGAA GGACACACTC CGTGATTGCA ACGAGGATTC CACCTGTTAC
    AACACAGTCA TCTCCCCCAT CTATTTTGTC TCTTTTGTGC TGACTGCCCA GTTTGTCCTG
    GTCAATGTGG TAATAGCGGT GCTCATGAAA CACCTTGAGG AAAGCAACAA GGAAGCTAAG
    GAGGAGGCGG AGCTGGAGGC AGAGATGGAA CTAGAGTTTC AGATCCAGAA CATGCAGGTT
    CCGGATCCTG ACCCATTTAT GTGGGCTGGG AGAGATCTCG GCTATCCCCA GGAAGGTTCT
    TACGGATATA TGAACTCCGT GCAGGACAAG GCCGACTCAC AGCTTTCATT AGTCAATCCC
    ATGGAAAGAC ACATGTTTGA TACAATATCA CTACTGATCC AGGGATCTTT GGAAGGAGAG
    ATGAAGCTGA TGGACAATTT GTCTGGCTCT GTGTGCCACC ATTACGCCCT CCCTCCCTCT
    GACAAGGGCT CCTCGGAAAA ACAGATCCCA TTGGCAGAGA TGGAAGGCCT ATCCATGACA
    TCAGACATTC TGTCCGAGAA ATCATGGTCC TTAGCTCTGA CGGACGACTC CCTGCCAGAT
    GACCCTAATC CCAACCCATT TGGTGTACTG GAAACCAAAC TTGGCCTCCC CGAGGAGAAA
    CATTCGGCAA CCGTTGAGGA GGCTACGAAT CTTCTCAGCG TCCGGAAACC GTCAGTATGC
    CGCACACATT CCCTGCCCAA TGACAGCTAC ATGTTGCAAA CGGGAAGCAC GTGCACTGAG
    TGCCAGGAGC AAAGAAACCA CTGTTACCAG AAATCAGGTT CCAGCTCAAC GGTTCAGTCT
    CAGCCAAATG ACCTCAACTC TCTTCTCCAA GTGCCACAAA TCTCCATCCC TTATGAAGCT
    TCAGGAAGTA CAGTGATATC AGAGATTCCC AAACTCCCCG CCCCCTTGCG CTCTCCATCG
    GCTAATAGGA TGCTCCGCAG ACAGGAAGCA ATCAGAACCG ACTCTTTGGA ATTTTTTCCC
    GATAGCAAAG AAAATGCTGG CATAACTGAG CCCCCACCTG TATATATTCC AATGTGCCAG
    GTGCCCACCG AGACACCCTC GGCTTTTATA GAACCAGAAA GAACTTTATG CCAGTTGAAG
    CGACGCAGTG TTTACACCCA GCAAGTCCAA GATACCTGCA AGATGTTTAG GTACCCTGAT
    GCTTCTCAAA CCAACATGGA CACGTTACGA GAAACCTCAA AGATTCCAGA GAAAGAAGAT
    TTGTTGAGCG CAGCATGTTG TAGGCGGCAT CTCAACTCCA AAACGTGTAA AGCATTAAGC
    GTCTATTCTG CAGCATACTC CTTAAGCCAG AAACTGAAAC TAGGTAACAG CGGCAACGTT
    ATTTTGAAGG ACATGAAGAG GTTCTACAGC GTAGATGCCC ACGGCGTCCT TAAAAAGACA
    TCCTCTTGGT TGGATGACCC CAGAAGACAT TCCATAGAAA TTTGTTCTTT AGAGAACACT
    CTTCATCACT ACTCTACTTC ACGTTCTTGT GGATTCATTA GCCACGTATT CCAGAAGAAG
    GAGGACTTGC AAATAGTGCA GAGGACGGGC GGACAGGATC CCGTCTGTCC AGATTGTGTT
    CACTCAGGAG AAGCATCAGA ACTACCACTC TCTTACGTCT CGAGTGGGCA ATCACAATCG
    ACAGACTCGA CCGAGCTCGG TAAAATAGAC TCTTTCGTTC CGGAAATGCA GACATCCCCT
    AAACCACTGG GAGATTTAGT TTCTGTCGAA AGTTTTTCTA TGGATGCAGT TGAATTAAAT
    CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51011
    Clone ID Related Accession (Same CDS sequence) XM_031894865.1 , XM_031894866.1 , XM_031894867.1 , XM_031894869.1 , XM_031894868.1 , XM_031894870.1
    Accession Version XM_031894869.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7086bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATCGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCCTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AGTAAGGAGA GAGAGCTGGC CGATCTGATG CTGGATGATG TAATATTGGA GCACTCAGCC
    AGTCCAGTTA AAGAGGCCCA GTGCAAACCC TACTACTCGG AGTATTCTCA CCCTCGGCTG
    CTCATCCATC AAATGTGCAC AAGCCATTAT CTGGATTTGT TTATTACTGG GGTCATCGGA
    TTGAATGTTA TCACTATGGC GATGGAGCAC TACCAGCAAC CCAAGGTATT GGATGAAGCT
    CTAAAAATCT GTAATTACAT ATTTACCCTC ATTTTTGTGC TGGAATCTGG GTGCAAAATG
    GTAGCTTTTG GCTTCCGGCG ATTCTTCAAA GACAGGTGGA ACCAGTTGGA TTTGGCCATT
    GTTCTGCTCT CCATTATGGG GATCACCCTG GAGGAGATTG AAGTCAATGC ATCGCTGCCC
    ATTAACCCAA CAATCATCAG AATAATGAGG GTCCTGCGGA TCGCTCGCGT GCTGAAGTTA
    TTAAAGATGG CAGTCGGTAT GCGAGCCCTG CTGGATACAG TATTGCAAGC GCTTCCTCAA
    GTGGGGAATC TCGGCCTGCT CTTTATGCTG CTGTTTTTCA TATTTGCCGC TTTGGGAGTT
    GAACTTTTTG GCGACTTAGA ATGCACTGAT TCCCACCCGT GTGAAGGTCT CGGCAGGCAC
    GCCACGTTCA GGAACTTCGG CATGGCCTTT CTGACTTTGT TCAGAGTATC CACAGGAGAT
    AACTGGAACG GTATAATGAA GGACACACTC CGTGATTGCA ACGAGGATTC CACCTGTTAC
    AACACAGTCA TCTCCCCCAT CTATTTTGTC TCTTTTGTGC TGACTGCCCA GTTTGTCCTG
    GTCAATGTGG TAATAGCGGT GCTCATGAAA CACCTTGAGG AAAGCAACAA GGAAGCTAAG
    GAGGAGGCGG AGCTGGAGGC AGAGATGGAA CTAGAGTTTC AGATCCAGAA CATGCAGGTT
    CCGGATCCTG ACCCATTTAT GTGGGCTGGG AGAGATCTCG GCTATCCCCA GGAAGGTTCT
    TACGGATATA TGAACTCCGT GCAGGACAAG GCCGACTCAC AGCTTTCATT AGTCAATCCC
    ATGGAAAGAC ACATGTTTGA TACAATATCA CTACTGATCC AGGGATCTTT GGAAGGAGAG
    ATGAAGCTGA TGGACAATTT GTCTGGCTCT GTGTGCCACC ATTACGCCCT CCCTCCCTCT
    GACAAGGGCT CCTCGGAAAA ACAGATCCCA TTGGCAGAGA TGGAAGGCCT ATCCATGACA
    TCAGACATTC TGTCCGAGAA ATCATGGTCC TTAGCTCTGA CGGACGACTC CCTGCCAGAT
    GACCCTAATC CCAACCCATT TGGTGTACTG GAAACCAAAC TTGGCCTCCC CGAGGAGAAA
    CATTCGGCAA CCGTTGAGGA GGCTACGAAT CTTCTCAGCG TCCGGAAACC GTCAGTATGC
    CGCACACATT CCCTGCCCAA TGACAGCTAC ATGTTGCAAA CGGGAAGCAC GTGCACTGAG
    TGCCAGGAGC AAAGAAACCA CTGTTACCAG AAATCAGGTT CCAGCTCAAC GGTTCAGTCT
    CAGCCAAATG ACCTCAACTC TCTTCTCCAA GTGCCACAAA TCTCCATCCC TTATGAAGCT
    TCAGGAAGTA CAGTGATATC AGAGATTCCC AAACTCCCCG CCCCCTTGCG CTCTCCATCG
    GCTAATAGGA TGCTCCGCAG ACAGGAAGCA ATCAGAACCG ACTCTTTGGA ATTTTTTCCC
    GATAGCAAAG AAAATGCTGG CATAACTGAG CCCCCACCTG TATATATTCC AATGTGCCAG
    GTGCCCACCG AGACACCCTC GGCTTTTATA GAACCAGAAA GAACTTTATG CCAGTTGAAG
    CGACGCAGTG TTTACACCCA GCAAGTCCAA GATACCTGCA AGATGTTTAG GTACCCTGAT
    GCTTCTCAAA CCAACATGGA CACGTTACGA GAAACCTCAA AGATTCCAGA GAAAGAAGAT
    TTGTTGAGCG CAGCATGTTG TAGGCGGCAT CTCAACTCCA AAACGTGTAA AGCATTAAGC
    GTCTATTCTG CAGCATACTC CTTAAGCCAG AAACTGAAAC TAGGTAACAG CGGCAACGTT
    ATTTTGAAGG ACATGAAGAG GTTCTACAGC GTAGATGCCC ACGGCGTCCT TAAAAAGACA
    TCCTCTTGGT TGGATGACCC CAGAAGACAT TCCATAGAAA TTTGTTCTTT AGAGAACACT
    CTTCATCACT ACTCTACTTC ACGTTCTTGT GGATTCATTA GCCACGTATT CCAGAAGAAG
    GAGGACTTGC AAATAGTGCA GAGGACGGGC GGACAGGATC CCGTCTGTCC AGATTGTGTT
    CACTCAGGAG AAGCATCAGA ACTACCACTC TCTTACGTCT CGAGTGGGCA ATCACAATCG
    ACAGACTCGA CCGAGCTCGG TAAAATAGAC TCTTTCGTTC CGGAAATGCA GACATCCCCT
    AAACCACTGG GAGATTTAGT TTCTGTCGAA AGTTTTTCTA TGGATGCAGT TGAATTAAAT
    CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750729.1
    CDS1990..9075
    Translation

    Target ORF information:

    RefSeq Version XM_031894869.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894869.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATCGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCCTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AGTAAGGAGA GAGAGCTGGC CGATCTGATG CTGGATGATG TAATATTGGA GCACTCAGCC
    AGTCCAGTTA AAGAGGCCCA GTGCAAACCC TACTACTCGG AGTATTCTCA CCCTCGGCTG
    CTCATCCATC AAATGTGCAC AAGCCATTAT CTGGATTTGT TTATTACTGG GGTCATCGGA
    TTGAATGTTA TCACTATGGC GATGGAGCAC TACCAGCAAC CCAAGGTATT GGATGAAGCT
    CTAAAAATCT GTAATTACAT ATTTACCCTC ATTTTTGTGC TGGAATCTGG GTGCAAAATG
    GTAGCTTTTG GCTTCCGGCG ATTCTTCAAA GACAGGTGGA ACCAGTTGGA TTTGGCCATT
    GTTCTGCTCT CCATTATGGG GATCACCCTG GAGGAGATTG AAGTCAATGC ATCGCTGCCC
    ATTAACCCAA CAATCATCAG AATAATGAGG GTCCTGCGGA TCGCTCGCGT GCTGAAGTTA
    TTAAAGATGG CAGTCGGTAT GCGAGCCCTG CTGGATACAG TATTGCAAGC GCTTCCTCAA
    GTGGGGAATC TCGGCCTGCT CTTTATGCTG CTGTTTTTCA TATTTGCCGC TTTGGGAGTT
    GAACTTTTTG GCGACTTAGA ATGCACTGAT TCCCACCCGT GTGAAGGTCT CGGCAGGCAC
    GCCACGTTCA GGAACTTCGG CATGGCCTTT CTGACTTTGT TCAGAGTATC CACAGGAGAT
    AACTGGAACG GTATAATGAA GGACACACTC CGTGATTGCA ACGAGGATTC CACCTGTTAC
    AACACAGTCA TCTCCCCCAT CTATTTTGTC TCTTTTGTGC TGACTGCCCA GTTTGTCCTG
    GTCAATGTGG TAATAGCGGT GCTCATGAAA CACCTTGAGG AAAGCAACAA GGAAGCTAAG
    GAGGAGGCGG AGCTGGAGGC AGAGATGGAA CTAGAGTTTC AGATCCAGAA CATGCAGGTT
    CCGGATCCTG ACCCATTTAT GTGGGCTGGG AGAGATCTCG GCTATCCCCA GGAAGGTTCT
    TACGGATATA TGAACTCCGT GCAGGACAAG GCCGACTCAC AGCTTTCATT AGTCAATCCC
    ATGGAAAGAC ACATGTTTGA TACAATATCA CTACTGATCC AGGGATCTTT GGAAGGAGAG
    ATGAAGCTGA TGGACAATTT GTCTGGCTCT GTGTGCCACC ATTACGCCCT CCCTCCCTCT
    GACAAGGGCT CCTCGGAAAA ACAGATCCCA TTGGCAGAGA TGGAAGGCCT ATCCATGACA
    TCAGACATTC TGTCCGAGAA ATCATGGTCC TTAGCTCTGA CGGACGACTC CCTGCCAGAT
    GACCCTAATC CCAACCCATT TGGTGTACTG GAAACCAAAC TTGGCCTCCC CGAGGAGAAA
    CATTCGGCAA CCGTTGAGGA GGCTACGAAT CTTCTCAGCG TCCGGAAACC GTCAGTATGC
    CGCACACATT CCCTGCCCAA TGACAGCTAC ATGTTGCAAA CGGGAAGCAC GTGCACTGAG
    TGCCAGGAGC AAAGAAACCA CTGTTACCAG AAATCAGGTT CCAGCTCAAC GGTTCAGTCT
    CAGCCAAATG ACCTCAACTC TCTTCTCCAA GTGCCACAAA TCTCCATCCC TTATGAAGCT
    TCAGGAAGTA CAGTGATATC AGAGATTCCC AAACTCCCCG CCCCCTTGCG CTCTCCATCG
    GCTAATAGGA TGCTCCGCAG ACAGGAAGCA ATCAGAACCG ACTCTTTGGA ATTTTTTCCC
    GATAGCAAAG AAAATGCTGG CATAACTGAG CCCCCACCTG TATATATTCC AATGTGCCAG
    GTGCCCACCG AGACACCCTC GGCTTTTATA GAACCAGAAA GAACTTTATG CCAGTTGAAG
    CGACGCAGTG TTTACACCCA GCAAGTCCAA GATACCTGCA AGATGTTTAG GTACCCTGAT
    GCTTCTCAAA CCAACATGGA CACGTTACGA GAAACCTCAA AGATTCCAGA GAAAGAAGAT
    TTGTTGAGCG CAGCATGTTG TAGGCGGCAT CTCAACTCCA AAACGTGTAA AGCATTAAGC
    GTCTATTCTG CAGCATACTC CTTAAGCCAG AAACTGAAAC TAGGTAACAG CGGCAACGTT
    ATTTTGAAGG ACATGAAGAG GTTCTACAGC GTAGATGCCC ACGGCGTCCT TAAAAAGACA
    TCCTCTTGGT TGGATGACCC CAGAAGACAT TCCATAGAAA TTTGTTCTTT AGAGAACACT
    CTTCATCACT ACTCTACTTC ACGTTCTTGT GGATTCATTA GCCACGTATT CCAGAAGAAG
    GAGGACTTGC AAATAGTGCA GAGGACGGGC GGACAGGATC CCGTCTGTCC AGATTGTGTT
    CACTCAGGAG AAGCATCAGA ACTACCACTC TCTTACGTCT CGAGTGGGCA ATCACAATCG
    ACAGACTCGA CCGAGCTCGG TAAAATAGAC TCTTTCGTTC CGGAAATGCA GACATCCCCT
    AAACCACTGG GAGATTTAGT TTCTGTCGAA AGTTTTTCTA TGGATGCAGT TGAATTAAAT
    CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51011
    Clone ID Related Accession (Same CDS sequence) XM_031894865.1 , XM_031894866.1 , XM_031894867.1 , XM_031894869.1 , XM_031894868.1 , XM_031894870.1
    Accession Version XM_031894868.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7086bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATCGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCCTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AGTAAGGAGA GAGAGCTGGC CGATCTGATG CTGGATGATG TAATATTGGA GCACTCAGCC
    AGTCCAGTTA AAGAGGCCCA GTGCAAACCC TACTACTCGG AGTATTCTCA CCCTCGGCTG
    CTCATCCATC AAATGTGCAC AAGCCATTAT CTGGATTTGT TTATTACTGG GGTCATCGGA
    TTGAATGTTA TCACTATGGC GATGGAGCAC TACCAGCAAC CCAAGGTATT GGATGAAGCT
    CTAAAAATCT GTAATTACAT ATTTACCCTC ATTTTTGTGC TGGAATCTGG GTGCAAAATG
    GTAGCTTTTG GCTTCCGGCG ATTCTTCAAA GACAGGTGGA ACCAGTTGGA TTTGGCCATT
    GTTCTGCTCT CCATTATGGG GATCACCCTG GAGGAGATTG AAGTCAATGC ATCGCTGCCC
    ATTAACCCAA CAATCATCAG AATAATGAGG GTCCTGCGGA TCGCTCGCGT GCTGAAGTTA
    TTAAAGATGG CAGTCGGTAT GCGAGCCCTG CTGGATACAG TATTGCAAGC GCTTCCTCAA
    GTGGGGAATC TCGGCCTGCT CTTTATGCTG CTGTTTTTCA TATTTGCCGC TTTGGGAGTT
    GAACTTTTTG GCGACTTAGA ATGCACTGAT TCCCACCCGT GTGAAGGTCT CGGCAGGCAC
    GCCACGTTCA GGAACTTCGG CATGGCCTTT CTGACTTTGT TCAGAGTATC CACAGGAGAT
    AACTGGAACG GTATAATGAA GGACACACTC CGTGATTGCA ACGAGGATTC CACCTGTTAC
    AACACAGTCA TCTCCCCCAT CTATTTTGTC TCTTTTGTGC TGACTGCCCA GTTTGTCCTG
    GTCAATGTGG TAATAGCGGT GCTCATGAAA CACCTTGAGG AAAGCAACAA GGAAGCTAAG
    GAGGAGGCGG AGCTGGAGGC AGAGATGGAA CTAGAGTTTC AGATCCAGAA CATGCAGGTT
    CCGGATCCTG ACCCATTTAT GTGGGCTGGG AGAGATCTCG GCTATCCCCA GGAAGGTTCT
    TACGGATATA TGAACTCCGT GCAGGACAAG GCCGACTCAC AGCTTTCATT AGTCAATCCC
    ATGGAAAGAC ACATGTTTGA TACAATATCA CTACTGATCC AGGGATCTTT GGAAGGAGAG
    ATGAAGCTGA TGGACAATTT GTCTGGCTCT GTGTGCCACC ATTACGCCCT CCCTCCCTCT
    GACAAGGGCT CCTCGGAAAA ACAGATCCCA TTGGCAGAGA TGGAAGGCCT ATCCATGACA
    TCAGACATTC TGTCCGAGAA ATCATGGTCC TTAGCTCTGA CGGACGACTC CCTGCCAGAT
    GACCCTAATC CCAACCCATT TGGTGTACTG GAAACCAAAC TTGGCCTCCC CGAGGAGAAA
    CATTCGGCAA CCGTTGAGGA GGCTACGAAT CTTCTCAGCG TCCGGAAACC GTCAGTATGC
    CGCACACATT CCCTGCCCAA TGACAGCTAC ATGTTGCAAA CGGGAAGCAC GTGCACTGAG
    TGCCAGGAGC AAAGAAACCA CTGTTACCAG AAATCAGGTT CCAGCTCAAC GGTTCAGTCT
    CAGCCAAATG ACCTCAACTC TCTTCTCCAA GTGCCACAAA TCTCCATCCC TTATGAAGCT
    TCAGGAAGTA CAGTGATATC AGAGATTCCC AAACTCCCCG CCCCCTTGCG CTCTCCATCG
    GCTAATAGGA TGCTCCGCAG ACAGGAAGCA ATCAGAACCG ACTCTTTGGA ATTTTTTCCC
    GATAGCAAAG AAAATGCTGG CATAACTGAG CCCCCACCTG TATATATTCC AATGTGCCAG
    GTGCCCACCG AGACACCCTC GGCTTTTATA GAACCAGAAA GAACTTTATG CCAGTTGAAG
    CGACGCAGTG TTTACACCCA GCAAGTCCAA GATACCTGCA AGATGTTTAG GTACCCTGAT
    GCTTCTCAAA CCAACATGGA CACGTTACGA GAAACCTCAA AGATTCCAGA GAAAGAAGAT
    TTGTTGAGCG CAGCATGTTG TAGGCGGCAT CTCAACTCCA AAACGTGTAA AGCATTAAGC
    GTCTATTCTG CAGCATACTC CTTAAGCCAG AAACTGAAAC TAGGTAACAG CGGCAACGTT
    ATTTTGAAGG ACATGAAGAG GTTCTACAGC GTAGATGCCC ACGGCGTCCT TAAAAAGACA
    TCCTCTTGGT TGGATGACCC CAGAAGACAT TCCATAGAAA TTTGTTCTTT AGAGAACACT
    CTTCATCACT ACTCTACTTC ACGTTCTTGT GGATTCATTA GCCACGTATT CCAGAAGAAG
    GAGGACTTGC AAATAGTGCA GAGGACGGGC GGACAGGATC CCGTCTGTCC AGATTGTGTT
    CACTCAGGAG AAGCATCAGA ACTACCACTC TCTTACGTCT CGAGTGGGCA ATCACAATCG
    ACAGACTCGA CCGAGCTCGG TAAAATAGAC TCTTTCGTTC CGGAAATGCA GACATCCCCT
    AAACCACTGG GAGATTTAGT TTCTGTCGAA AGTTTTTCTA TGGATGCAGT TGAATTAAAT
    CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750728.1
    CDS1966..9051
    Translation

    Target ORF information:

    RefSeq Version XM_031894868.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894868.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATCGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCCTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AGTAAGGAGA GAGAGCTGGC CGATCTGATG CTGGATGATG TAATATTGGA GCACTCAGCC
    AGTCCAGTTA AAGAGGCCCA GTGCAAACCC TACTACTCGG AGTATTCTCA CCCTCGGCTG
    CTCATCCATC AAATGTGCAC AAGCCATTAT CTGGATTTGT TTATTACTGG GGTCATCGGA
    TTGAATGTTA TCACTATGGC GATGGAGCAC TACCAGCAAC CCAAGGTATT GGATGAAGCT
    CTAAAAATCT GTAATTACAT ATTTACCCTC ATTTTTGTGC TGGAATCTGG GTGCAAAATG
    GTAGCTTTTG GCTTCCGGCG ATTCTTCAAA GACAGGTGGA ACCAGTTGGA TTTGGCCATT
    GTTCTGCTCT CCATTATGGG GATCACCCTG GAGGAGATTG AAGTCAATGC ATCGCTGCCC
    ATTAACCCAA CAATCATCAG AATAATGAGG GTCCTGCGGA TCGCTCGCGT GCTGAAGTTA
    TTAAAGATGG CAGTCGGTAT GCGAGCCCTG CTGGATACAG TATTGCAAGC GCTTCCTCAA
    GTGGGGAATC TCGGCCTGCT CTTTATGCTG CTGTTTTTCA TATTTGCCGC TTTGGGAGTT
    GAACTTTTTG GCGACTTAGA ATGCACTGAT TCCCACCCGT GTGAAGGTCT CGGCAGGCAC
    GCCACGTTCA GGAACTTCGG CATGGCCTTT CTGACTTTGT TCAGAGTATC CACAGGAGAT
    AACTGGAACG GTATAATGAA GGACACACTC CGTGATTGCA ACGAGGATTC CACCTGTTAC
    AACACAGTCA TCTCCCCCAT CTATTTTGTC TCTTTTGTGC TGACTGCCCA GTTTGTCCTG
    GTCAATGTGG TAATAGCGGT GCTCATGAAA CACCTTGAGG AAAGCAACAA GGAAGCTAAG
    GAGGAGGCGG AGCTGGAGGC AGAGATGGAA CTAGAGTTTC AGATCCAGAA CATGCAGGTT
    CCGGATCCTG ACCCATTTAT GTGGGCTGGG AGAGATCTCG GCTATCCCCA GGAAGGTTCT
    TACGGATATA TGAACTCCGT GCAGGACAAG GCCGACTCAC AGCTTTCATT AGTCAATCCC
    ATGGAAAGAC ACATGTTTGA TACAATATCA CTACTGATCC AGGGATCTTT GGAAGGAGAG
    ATGAAGCTGA TGGACAATTT GTCTGGCTCT GTGTGCCACC ATTACGCCCT CCCTCCCTCT
    GACAAGGGCT CCTCGGAAAA ACAGATCCCA TTGGCAGAGA TGGAAGGCCT ATCCATGACA
    TCAGACATTC TGTCCGAGAA ATCATGGTCC TTAGCTCTGA CGGACGACTC CCTGCCAGAT
    GACCCTAATC CCAACCCATT TGGTGTACTG GAAACCAAAC TTGGCCTCCC CGAGGAGAAA
    CATTCGGCAA CCGTTGAGGA GGCTACGAAT CTTCTCAGCG TCCGGAAACC GTCAGTATGC
    CGCACACATT CCCTGCCCAA TGACAGCTAC ATGTTGCAAA CGGGAAGCAC GTGCACTGAG
    TGCCAGGAGC AAAGAAACCA CTGTTACCAG AAATCAGGTT CCAGCTCAAC GGTTCAGTCT
    CAGCCAAATG ACCTCAACTC TCTTCTCCAA GTGCCACAAA TCTCCATCCC TTATGAAGCT
    TCAGGAAGTA CAGTGATATC AGAGATTCCC AAACTCCCCG CCCCCTTGCG CTCTCCATCG
    GCTAATAGGA TGCTCCGCAG ACAGGAAGCA ATCAGAACCG ACTCTTTGGA ATTTTTTCCC
    GATAGCAAAG AAAATGCTGG CATAACTGAG CCCCCACCTG TATATATTCC AATGTGCCAG
    GTGCCCACCG AGACACCCTC GGCTTTTATA GAACCAGAAA GAACTTTATG CCAGTTGAAG
    CGACGCAGTG TTTACACCCA GCAAGTCCAA GATACCTGCA AGATGTTTAG GTACCCTGAT
    GCTTCTCAAA CCAACATGGA CACGTTACGA GAAACCTCAA AGATTCCAGA GAAAGAAGAT
    TTGTTGAGCG CAGCATGTTG TAGGCGGCAT CTCAACTCCA AAACGTGTAA AGCATTAAGC
    GTCTATTCTG CAGCATACTC CTTAAGCCAG AAACTGAAAC TAGGTAACAG CGGCAACGTT
    ATTTTGAAGG ACATGAAGAG GTTCTACAGC GTAGATGCCC ACGGCGTCCT TAAAAAGACA
    TCCTCTTGGT TGGATGACCC CAGAAGACAT TCCATAGAAA TTTGTTCTTT AGAGAACACT
    CTTCATCACT ACTCTACTTC ACGTTCTTGT GGATTCATTA GCCACGTATT CCAGAAGAAG
    GAGGACTTGC AAATAGTGCA GAGGACGGGC GGACAGGATC CCGTCTGTCC AGATTGTGTT
    CACTCAGGAG AAGCATCAGA ACTACCACTC TCTTACGTCT CGAGTGGGCA ATCACAATCG
    ACAGACTCGA CCGAGCTCGG TAAAATAGAC TCTTTCGTTC CGGAAATGCA GACATCCCCT
    AAACCACTGG GAGATTTAGT TTCTGTCGAA AGTTTTTCTA TGGATGCAGT TGAATTAAAT
    CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51011
    Clone ID Related Accession (Same CDS sequence) XM_031894865.1 , XM_031894866.1 , XM_031894867.1 , XM_031894869.1 , XM_031894868.1 , XM_031894870.1
    Accession Version XM_031894870.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7086bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATCGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCCTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AGTAAGGAGA GAGAGCTGGC CGATCTGATG CTGGATGATG TAATATTGGA GCACTCAGCC
    AGTCCAGTTA AAGAGGCCCA GTGCAAACCC TACTACTCGG AGTATTCTCA CCCTCGGCTG
    CTCATCCATC AAATGTGCAC AAGCCATTAT CTGGATTTGT TTATTACTGG GGTCATCGGA
    TTGAATGTTA TCACTATGGC GATGGAGCAC TACCAGCAAC CCAAGGTATT GGATGAAGCT
    CTAAAAATCT GTAATTACAT ATTTACCCTC ATTTTTGTGC TGGAATCTGG GTGCAAAATG
    GTAGCTTTTG GCTTCCGGCG ATTCTTCAAA GACAGGTGGA ACCAGTTGGA TTTGGCCATT
    GTTCTGCTCT CCATTATGGG GATCACCCTG GAGGAGATTG AAGTCAATGC ATCGCTGCCC
    ATTAACCCAA CAATCATCAG AATAATGAGG GTCCTGCGGA TCGCTCGCGT GCTGAAGTTA
    TTAAAGATGG CAGTCGGTAT GCGAGCCCTG CTGGATACAG TATTGCAAGC GCTTCCTCAA
    GTGGGGAATC TCGGCCTGCT CTTTATGCTG CTGTTTTTCA TATTTGCCGC TTTGGGAGTT
    GAACTTTTTG GCGACTTAGA ATGCACTGAT TCCCACCCGT GTGAAGGTCT CGGCAGGCAC
    GCCACGTTCA GGAACTTCGG CATGGCCTTT CTGACTTTGT TCAGAGTATC CACAGGAGAT
    AACTGGAACG GTATAATGAA GGACACACTC CGTGATTGCA ACGAGGATTC CACCTGTTAC
    AACACAGTCA TCTCCCCCAT CTATTTTGTC TCTTTTGTGC TGACTGCCCA GTTTGTCCTG
    GTCAATGTGG TAATAGCGGT GCTCATGAAA CACCTTGAGG AAAGCAACAA GGAAGCTAAG
    GAGGAGGCGG AGCTGGAGGC AGAGATGGAA CTAGAGTTTC AGATCCAGAA CATGCAGGTT
    CCGGATCCTG ACCCATTTAT GTGGGCTGGG AGAGATCTCG GCTATCCCCA GGAAGGTTCT
    TACGGATATA TGAACTCCGT GCAGGACAAG GCCGACTCAC AGCTTTCATT AGTCAATCCC
    ATGGAAAGAC ACATGTTTGA TACAATATCA CTACTGATCC AGGGATCTTT GGAAGGAGAG
    ATGAAGCTGA TGGACAATTT GTCTGGCTCT GTGTGCCACC ATTACGCCCT CCCTCCCTCT
    GACAAGGGCT CCTCGGAAAA ACAGATCCCA TTGGCAGAGA TGGAAGGCCT ATCCATGACA
    TCAGACATTC TGTCCGAGAA ATCATGGTCC TTAGCTCTGA CGGACGACTC CCTGCCAGAT
    GACCCTAATC CCAACCCATT TGGTGTACTG GAAACCAAAC TTGGCCTCCC CGAGGAGAAA
    CATTCGGCAA CCGTTGAGGA GGCTACGAAT CTTCTCAGCG TCCGGAAACC GTCAGTATGC
    CGCACACATT CCCTGCCCAA TGACAGCTAC ATGTTGCAAA CGGGAAGCAC GTGCACTGAG
    TGCCAGGAGC AAAGAAACCA CTGTTACCAG AAATCAGGTT CCAGCTCAAC GGTTCAGTCT
    CAGCCAAATG ACCTCAACTC TCTTCTCCAA GTGCCACAAA TCTCCATCCC TTATGAAGCT
    TCAGGAAGTA CAGTGATATC AGAGATTCCC AAACTCCCCG CCCCCTTGCG CTCTCCATCG
    GCTAATAGGA TGCTCCGCAG ACAGGAAGCA ATCAGAACCG ACTCTTTGGA ATTTTTTCCC
    GATAGCAAAG AAAATGCTGG CATAACTGAG CCCCCACCTG TATATATTCC AATGTGCCAG
    GTGCCCACCG AGACACCCTC GGCTTTTATA GAACCAGAAA GAACTTTATG CCAGTTGAAG
    CGACGCAGTG TTTACACCCA GCAAGTCCAA GATACCTGCA AGATGTTTAG GTACCCTGAT
    GCTTCTCAAA CCAACATGGA CACGTTACGA GAAACCTCAA AGATTCCAGA GAAAGAAGAT
    TTGTTGAGCG CAGCATGTTG TAGGCGGCAT CTCAACTCCA AAACGTGTAA AGCATTAAGC
    GTCTATTCTG CAGCATACTC CTTAAGCCAG AAACTGAAAC TAGGTAACAG CGGCAACGTT
    ATTTTGAAGG ACATGAAGAG GTTCTACAGC GTAGATGCCC ACGGCGTCCT TAAAAAGACA
    TCCTCTTGGT TGGATGACCC CAGAAGACAT TCCATAGAAA TTTGTTCTTT AGAGAACACT
    CTTCATCACT ACTCTACTTC ACGTTCTTGT GGATTCATTA GCCACGTATT CCAGAAGAAG
    GAGGACTTGC AAATAGTGCA GAGGACGGGC GGACAGGATC CCGTCTGTCC AGATTGTGTT
    CACTCAGGAG AAGCATCAGA ACTACCACTC TCTTACGTCT CGAGTGGGCA ATCACAATCG
    ACAGACTCGA CCGAGCTCGG TAAAATAGAC TCTTTCGTTC CGGAAATGCA GACATCCCCT
    AAACCACTGG GAGATTTAGT TTCTGTCGAA AGTTTTTCTA TGGATGCAGT TGAATTAAAT
    CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750730.1
    CDS1958..9043
    Translation

    Target ORF information:

    RefSeq Version XM_031894870.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894870.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATCGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCCTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AGTAAGGAGA GAGAGCTGGC CGATCTGATG CTGGATGATG TAATATTGGA GCACTCAGCC
    AGTCCAGTTA AAGAGGCCCA GTGCAAACCC TACTACTCGG AGTATTCTCA CCCTCGGCTG
    CTCATCCATC AAATGTGCAC AAGCCATTAT CTGGATTTGT TTATTACTGG GGTCATCGGA
    TTGAATGTTA TCACTATGGC GATGGAGCAC TACCAGCAAC CCAAGGTATT GGATGAAGCT
    CTAAAAATCT GTAATTACAT ATTTACCCTC ATTTTTGTGC TGGAATCTGG GTGCAAAATG
    GTAGCTTTTG GCTTCCGGCG ATTCTTCAAA GACAGGTGGA ACCAGTTGGA TTTGGCCATT
    GTTCTGCTCT CCATTATGGG GATCACCCTG GAGGAGATTG AAGTCAATGC ATCGCTGCCC
    ATTAACCCAA CAATCATCAG AATAATGAGG GTCCTGCGGA TCGCTCGCGT GCTGAAGTTA
    TTAAAGATGG CAGTCGGTAT GCGAGCCCTG CTGGATACAG TATTGCAAGC GCTTCCTCAA
    GTGGGGAATC TCGGCCTGCT CTTTATGCTG CTGTTTTTCA TATTTGCCGC TTTGGGAGTT
    GAACTTTTTG GCGACTTAGA ATGCACTGAT TCCCACCCGT GTGAAGGTCT CGGCAGGCAC
    GCCACGTTCA GGAACTTCGG CATGGCCTTT CTGACTTTGT TCAGAGTATC CACAGGAGAT
    AACTGGAACG GTATAATGAA GGACACACTC CGTGATTGCA ACGAGGATTC CACCTGTTAC
    AACACAGTCA TCTCCCCCAT CTATTTTGTC TCTTTTGTGC TGACTGCCCA GTTTGTCCTG
    GTCAATGTGG TAATAGCGGT GCTCATGAAA CACCTTGAGG AAAGCAACAA GGAAGCTAAG
    GAGGAGGCGG AGCTGGAGGC AGAGATGGAA CTAGAGTTTC AGATCCAGAA CATGCAGGTT
    CCGGATCCTG ACCCATTTAT GTGGGCTGGG AGAGATCTCG GCTATCCCCA GGAAGGTTCT
    TACGGATATA TGAACTCCGT GCAGGACAAG GCCGACTCAC AGCTTTCATT AGTCAATCCC
    ATGGAAAGAC ACATGTTTGA TACAATATCA CTACTGATCC AGGGATCTTT GGAAGGAGAG
    ATGAAGCTGA TGGACAATTT GTCTGGCTCT GTGTGCCACC ATTACGCCCT CCCTCCCTCT
    GACAAGGGCT CCTCGGAAAA ACAGATCCCA TTGGCAGAGA TGGAAGGCCT ATCCATGACA
    TCAGACATTC TGTCCGAGAA ATCATGGTCC TTAGCTCTGA CGGACGACTC CCTGCCAGAT
    GACCCTAATC CCAACCCATT TGGTGTACTG GAAACCAAAC TTGGCCTCCC CGAGGAGAAA
    CATTCGGCAA CCGTTGAGGA GGCTACGAAT CTTCTCAGCG TCCGGAAACC GTCAGTATGC
    CGCACACATT CCCTGCCCAA TGACAGCTAC ATGTTGCAAA CGGGAAGCAC GTGCACTGAG
    TGCCAGGAGC AAAGAAACCA CTGTTACCAG AAATCAGGTT CCAGCTCAAC GGTTCAGTCT
    CAGCCAAATG ACCTCAACTC TCTTCTCCAA GTGCCACAAA TCTCCATCCC TTATGAAGCT
    TCAGGAAGTA CAGTGATATC AGAGATTCCC AAACTCCCCG CCCCCTTGCG CTCTCCATCG
    GCTAATAGGA TGCTCCGCAG ACAGGAAGCA ATCAGAACCG ACTCTTTGGA ATTTTTTCCC
    GATAGCAAAG AAAATGCTGG CATAACTGAG CCCCCACCTG TATATATTCC AATGTGCCAG
    GTGCCCACCG AGACACCCTC GGCTTTTATA GAACCAGAAA GAACTTTATG CCAGTTGAAG
    CGACGCAGTG TTTACACCCA GCAAGTCCAA GATACCTGCA AGATGTTTAG GTACCCTGAT
    GCTTCTCAAA CCAACATGGA CACGTTACGA GAAACCTCAA AGATTCCAGA GAAAGAAGAT
    TTGTTGAGCG CAGCATGTTG TAGGCGGCAT CTCAACTCCA AAACGTGTAA AGCATTAAGC
    GTCTATTCTG CAGCATACTC CTTAAGCCAG AAACTGAAAC TAGGTAACAG CGGCAACGTT
    ATTTTGAAGG ACATGAAGAG GTTCTACAGC GTAGATGCCC ACGGCGTCCT TAAAAAGACA
    TCCTCTTGGT TGGATGACCC CAGAAGACAT TCCATAGAAA TTTGTTCTTT AGAGAACACT
    CTTCATCACT ACTCTACTTC ACGTTCTTGT GGATTCATTA GCCACGTATT CCAGAAGAAG
    GAGGACTTGC AAATAGTGCA GAGGACGGGC GGACAGGATC CCGTCTGTCC AGATTGTGTT
    CACTCAGGAG AAGCATCAGA ACTACCACTC TCTTACGTCT CGAGTGGGCA ATCACAATCG
    ACAGACTCGA CCGAGCTCGG TAAAATAGAC TCTTTCGTTC CGGAAATGCA GACATCCCCT
    AAACCACTGG GAGATTTAGT TTCTGTCGAA AGTTTTTCTA TGGATGCAGT TGAATTAAAT
    CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51012
    Clone ID Related Accession (Same CDS sequence) XM_031894871.1
    Accession Version XM_031894871.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATCGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCCTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AATCTGATGC TGGATGATGT AATATTGGAG CACTCAGCCA GTCCAGTTAA AGAGGCCCAG
    TGCAAACCCT ACTACTCGGA GTATTCTCAC CCTCGGCTGC TCATCCATCA AATGTGCACA
    AGCCATTATC TGGATTTGTT TATTACTGGG GTCATCGGAT TGAATGTTAT CACTATGGCG
    ATGGAGCACT ACCAGCAACC CAAGGTATTG GATGAAGCTC TAAAAATCTG TAATTACATA
    TTTACCCTCA TTTTTGTGCT GGAATCTGGG TGCAAAATGG TAGCTTTTGG CTTCCGGCGA
    TTCTTCAAAG ACAGGTGGAA CCAGTTGGAT TTGGCCATTG TTCTGCTCTC CATTATGGGG
    ATCACCCTGG AGGAGATTGA AGTCAATGCA TCGCTGCCCA TTAACCCAAC AATCATCAGA
    ATAATGAGGG TCCTGCGGAT CGCTCGCGTG CTGAAGTTAT TAAAGATGGC AGTCGGTATG
    CGAGCCCTGC TGGATACAGT ATTGCAAGCG CTTCCTCAAG TGGGGAATCT CGGCCTGCTC
    TTTATGCTGC TGTTTTTCAT ATTTGCCGCT TTGGGAGTTG AACTTTTTGG CGACTTAGAA
    TGCACTGATT CCCACCCGTG TGAAGGTCTC GGCAGGCACG CCACGTTCAG GAACTTCGGC
    ATGGCCTTTC TGACTTTGTT CAGAGTATCC ACAGGAGATA ACTGGAACGG TATAATGAAG
    GACACACTCC GTGATTGCAA CGAGGATTCC ACCTGTTACA ACACAGTCAT CTCCCCCATC
    TATTTTGTCT CTTTTGTGCT GACTGCCCAG TTTGTCCTGG TCAATGTGGT AATAGCGGTG
    CTCATGAAAC ACCTTGAGGA AAGCAACAAG GAAGCTAAGG AGGAGGCGGA GCTGGAGGCA
    GAGATGGAAC TAGAGTTTCA GATCCAGAAC ATGCAGGTTC CGGATCCTGA CCCATTTATG
    TGGGCTGGGA GAGATCTCGG CTATCCCCAG GAAGGTTCTT ACGGATATAT GAACTCCGTG
    CAGGACAAGG CCGACTCACA GCTTTCATTA GTCAATCCCA TGGAAAGACA CATGTTTGAT
    ACAATATCAC TACTGATCCA GGGATCTTTG GAAGGAGAGA TGAAGCTGAT GGACAATTTG
    TCTGGCTCTG TGTGCCACCA TTACGCCCTC CCTCCCTCTG ACAAGGGCTC CTCGGAAAAA
    CAGATCCCAT TGGCAGAGAT GGAAGGCCTA TCCATGACAT CAGACATTCT GTCCGAGAAA
    TCATGGTCCT TAGCTCTGAC GGACGACTCC CTGCCAGATG ACCCTAATCC CAACCCATTT
    GGTGTACTGG AAACCAAACT TGGCCTCCCC GAGGAGAAAC ATTCGGCAAC CGTTGAGGAG
    GCTACGAATC TTCTCAGCGT CCGGAAACCG TCAGTATGCC GCACACATTC CCTGCCCAAT
    GACAGCTACA TGTTGCAAAC GGGAAGCACG TGCACTGAGT GCCAGGAGCA AAGAAACCAC
    TGTTACCAGA AATCAGGTTC CAGCTCAACG GTTCAGTCTC AGCCAAATGA CCTCAACTCT
    CTTCTCCAAG TGCCACAAAT CTCCATCCCT TATGAAGCTT CAGGAAGTAC AGTGATATCA
    GAGATTCCCA AACTCCCCGC CCCCTTGCGC TCTCCATCGG CTAATAGGAT GCTCCGCAGA
    CAGGAAGCAA TCAGAACCGA CTCTTTGGAA TTTTTTCCCG ATAGCAAAGA AAATGCTGGC
    ATAACTGAGC CCCCACCTGT ATATATTCCA ATGTGCCAGG TGCCCACCGA GACACCCTCG
    GCTTTTATAG AACCAGAAAG AACTTTATGC CAGTTGAAGC GACGCAGTGT TTACACCCAG
    CAAGTCCAAG ATACCTGCAA GATGTTTAGG TACCCTGATG CTTCTCAAAC CAACATGGAC
    ACGTTACGAG AAACCTCAAA GATTCCAGAG AAAGAAGATT TGTTGAGCGC AGCATGTTGT
    AGGCGGCATC TCAACTCCAA AACGTGTAAA GCATTAAGCG TCTATTCTGC AGCATACTCC
    TTAAGCCAGA AACTGAAACT AGGTAACAGC GGCAACGTTA TTTTGAAGGA CATGAAGAGG
    TTCTACAGCG TAGATGCCCA CGGCGTCCTT AAAAAGACAT CCTCTTGGTT GGATGACCCC
    AGAAGACATT CCATAGAAAT TTGTTCTTTA GAGAACACTC TTCATCACTA CTCTACTTCA
    CGTTCTTGTG GATTCATTAG CCACGTATTC CAGAAGAAGG AGGACTTGCA AATAGTGCAG
    AGGACGGGCG GACAGGATCC CGTCTGTCCA GATTGTGTTC ACTCAGGAGA AGCATCAGAA
    CTACCACTCT CTTACGTCTC GAGTGGGCAA TCACAATCGA CAGACTCGAC CGAGCTCGGT
    AAAATAGACT CTTTCGTTCC GGAAATGCAG ACATCCCCTA AACCACTGGG AGATTTAGTT
    TCTGTCGAAA GTTTTTCTAT GGATGCAGTT GAATTAAATC AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750731.1
    CDS2032..9096
    Translation

    Target ORF information:

    RefSeq Version XM_031894871.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894871.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATCGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCCTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AATCTGATGC TGGATGATGT AATATTGGAG CACTCAGCCA GTCCAGTTAA AGAGGCCCAG
    TGCAAACCCT ACTACTCGGA GTATTCTCAC CCTCGGCTGC TCATCCATCA AATGTGCACA
    AGCCATTATC TGGATTTGTT TATTACTGGG GTCATCGGAT TGAATGTTAT CACTATGGCG
    ATGGAGCACT ACCAGCAACC CAAGGTATTG GATGAAGCTC TAAAAATCTG TAATTACATA
    TTTACCCTCA TTTTTGTGCT GGAATCTGGG TGCAAAATGG TAGCTTTTGG CTTCCGGCGA
    TTCTTCAAAG ACAGGTGGAA CCAGTTGGAT TTGGCCATTG TTCTGCTCTC CATTATGGGG
    ATCACCCTGG AGGAGATTGA AGTCAATGCA TCGCTGCCCA TTAACCCAAC AATCATCAGA
    ATAATGAGGG TCCTGCGGAT CGCTCGCGTG CTGAAGTTAT TAAAGATGGC AGTCGGTATG
    CGAGCCCTGC TGGATACAGT ATTGCAAGCG CTTCCTCAAG TGGGGAATCT CGGCCTGCTC
    TTTATGCTGC TGTTTTTCAT ATTTGCCGCT TTGGGAGTTG AACTTTTTGG CGACTTAGAA
    TGCACTGATT CCCACCCGTG TGAAGGTCTC GGCAGGCACG CCACGTTCAG GAACTTCGGC
    ATGGCCTTTC TGACTTTGTT CAGAGTATCC ACAGGAGATA ACTGGAACGG TATAATGAAG
    GACACACTCC GTGATTGCAA CGAGGATTCC ACCTGTTACA ACACAGTCAT CTCCCCCATC
    TATTTTGTCT CTTTTGTGCT GACTGCCCAG TTTGTCCTGG TCAATGTGGT AATAGCGGTG
    CTCATGAAAC ACCTTGAGGA AAGCAACAAG GAAGCTAAGG AGGAGGCGGA GCTGGAGGCA
    GAGATGGAAC TAGAGTTTCA GATCCAGAAC ATGCAGGTTC CGGATCCTGA CCCATTTATG
    TGGGCTGGGA GAGATCTCGG CTATCCCCAG GAAGGTTCTT ACGGATATAT GAACTCCGTG
    CAGGACAAGG CCGACTCACA GCTTTCATTA GTCAATCCCA TGGAAAGACA CATGTTTGAT
    ACAATATCAC TACTGATCCA GGGATCTTTG GAAGGAGAGA TGAAGCTGAT GGACAATTTG
    TCTGGCTCTG TGTGCCACCA TTACGCCCTC CCTCCCTCTG ACAAGGGCTC CTCGGAAAAA
    CAGATCCCAT TGGCAGAGAT GGAAGGCCTA TCCATGACAT CAGACATTCT GTCCGAGAAA
    TCATGGTCCT TAGCTCTGAC GGACGACTCC CTGCCAGATG ACCCTAATCC CAACCCATTT
    GGTGTACTGG AAACCAAACT TGGCCTCCCC GAGGAGAAAC ATTCGGCAAC CGTTGAGGAG
    GCTACGAATC TTCTCAGCGT CCGGAAACCG TCAGTATGCC GCACACATTC CCTGCCCAAT
    GACAGCTACA TGTTGCAAAC GGGAAGCACG TGCACTGAGT GCCAGGAGCA AAGAAACCAC
    TGTTACCAGA AATCAGGTTC CAGCTCAACG GTTCAGTCTC AGCCAAATGA CCTCAACTCT
    CTTCTCCAAG TGCCACAAAT CTCCATCCCT TATGAAGCTT CAGGAAGTAC AGTGATATCA
    GAGATTCCCA AACTCCCCGC CCCCTTGCGC TCTCCATCGG CTAATAGGAT GCTCCGCAGA
    CAGGAAGCAA TCAGAACCGA CTCTTTGGAA TTTTTTCCCG ATAGCAAAGA AAATGCTGGC
    ATAACTGAGC CCCCACCTGT ATATATTCCA ATGTGCCAGG TGCCCACCGA GACACCCTCG
    GCTTTTATAG AACCAGAAAG AACTTTATGC CAGTTGAAGC GACGCAGTGT TTACACCCAG
    CAAGTCCAAG ATACCTGCAA GATGTTTAGG TACCCTGATG CTTCTCAAAC CAACATGGAC
    ACGTTACGAG AAACCTCAAA GATTCCAGAG AAAGAAGATT TGTTGAGCGC AGCATGTTGT
    AGGCGGCATC TCAACTCCAA AACGTGTAAA GCATTAAGCG TCTATTCTGC AGCATACTCC
    TTAAGCCAGA AACTGAAACT AGGTAACAGC GGCAACGTTA TTTTGAAGGA CATGAAGAGG
    TTCTACAGCG TAGATGCCCA CGGCGTCCTT AAAAAGACAT CCTCTTGGTT GGATGACCCC
    AGAAGACATT CCATAGAAAT TTGTTCTTTA GAGAACACTC TTCATCACTA CTCTACTTCA
    CGTTCTTGTG GATTCATTAG CCACGTATTC CAGAAGAAGG AGGACTTGCA AATAGTGCAG
    AGGACGGGCG GACAGGATCC CGTCTGTCCA GATTGTGTTC ACTCAGGAGA AGCATCAGAA
    CTACCACTCT CTTACGTCTC GAGTGGGCAA TCACAATCGA CAGACTCGAC CGAGCTCGGT
    AAAATAGACT CTTTCGTTCC GGAAATGCAG ACATCCCCTA AACCACTGGG AGATTTAGTT
    TCTGTCGAAA GTTTTTCTAT GGATGCAGTT GAATTAAATC AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51013
    Clone ID Related Accession (Same CDS sequence) XM_031894872.1
    Accession Version XM_031894872.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7047bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATCGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCCTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AGTAAGGAGA GAGAGCTGGC CGATCTGATG CTGGATGATG TAATATTGGA GCACTCAGCC
    AGTCCAGTTA AAGAGGCCCA GTGCAAACCC TACTACTCGG AGTATTCTCA CCCTCGGCTG
    CTCATCCATC AAATGTGCAC AAGCCATTAT CTGGATTTGT TTATTACTGG GGTCATCGGA
    TTGAATGTTA TCACTATGGC GATGGAGCAC TACCAGCAAC CCAAGGTATT GGATGAAGCT
    CTAAAAATCT GTAATTACAT ATTTACCCTC ATTTTTGTGC TGGAATCTGG GTGCAAAATG
    GTAGCTTTTG GCTTCCGGCG ATTCTTCAAA GACAGGTGGA ACCAGTTGGA TTTGGCCATT
    GTTCTGCTCT CCATTATGGG GATCACCCTG GAGGAGATTG AAGTCAATGC ATCGCTGCCC
    ATTAACCCAA CAATCATCAG AATAATGAGG GTCCTGCGGA TCGCTCGCGT GCTGAAGTTA
    TTAAAGATGG CAGTCGGTAT GCGAGCCCTG CTGGATACAG TATTGCAAGC GCTTCCTCAA
    GTGGGGAATC TCGGCCTGCT CTTTATGCTG CTGTTTTTCA TATTTGCCGC TTTGGGAGTT
    GAACTTTTTG GCGACTTAGA ATGCACTGAT TCCCACCCGT GTGAAGGTCT CGGCAGGCAC
    GCCACGTTCA GGAACTTCGG CATGGCCTTT CTGACTTTGT TCAGAGTATC CACAGGAGAT
    AACTGGAACG GTATAATGAA GGACACACTC CGTGATTGCA ACGAGGATTC CACCTGTTAC
    AACACAGTCA TCTCCCCCAT CTATTTTGTC TCTTTTGTGC TGACTGCCCA GTTTGTCCTG
    GTCAATGTGG TAATAGCGGT GCTCATGAAA CACCTTGAGG AAAGCAACAA GGAAGCTAAG
    GAGGAGGCGG AGCTGGAGGC AGAGATGGAA CTAGAGTTTC AGATCCAGAA CATGCAGGTT
    CCGGATCCTG ACCCATTTAT GTGGGCTGGG AGAGATCTCG GCTATCCCCA GGAAGGTTCT
    TACGGATATA TGAACTCCGT GCAGGACAAG GCCGACTCAC AGCTTTCATT AGTCAATCCC
    ATGGGATCTT TGGAAGGAGA GATGAAGCTG ATGGACAATT TGTCTGGCTC TGTGTGCCAC
    CATTACGCCC TCCCTCCCTC TGACAAGGGC TCCTCGGAAA AACAGATCCC ATTGGCAGAG
    ATGGAAGGCC TATCCATGAC ATCAGACATT CTGTCCGAGA AATCATGGTC CTTAGCTCTG
    ACGGACGACT CCCTGCCAGA TGACCCTAAT CCCAACCCAT TTGGTGTACT GGAAACCAAA
    CTTGGCCTCC CCGAGGAGAA ACATTCGGCA ACCGTTGAGG AGGCTACGAA TCTTCTCAGC
    GTCCGGAAAC CGTCAGTATG CCGCACACAT TCCCTGCCCA ATGACAGCTA CATGTTGCAA
    ACGGGAAGCA CGTGCACTGA GTGCCAGGAG CAAAGAAACC ACTGTTACCA GAAATCAGGT
    TCCAGCTCAA CGGTTCAGTC TCAGCCAAAT GACCTCAACT CTCTTCTCCA AGTGCCACAA
    ATCTCCATCC CTTATGAAGC TTCAGGAAGT ACAGTGATAT CAGAGATTCC CAAACTCCCC
    GCCCCCTTGC GCTCTCCATC GGCTAATAGG ATGCTCCGCA GACAGGAAGC AATCAGAACC
    GACTCTTTGG AATTTTTTCC CGATAGCAAA GAAAATGCTG GCATAACTGA GCCCCCACCT
    GTATATATTC CAATGTGCCA GGTGCCCACC GAGACACCCT CGGCTTTTAT AGAACCAGAA
    AGAACTTTAT GCCAGTTGAA GCGACGCAGT GTTTACACCC AGCAAGTCCA AGATACCTGC
    AAGATGTTTA GGTACCCTGA TGCTTCTCAA ACCAACATGG ACACGTTACG AGAAACCTCA
    AAGATTCCAG AGAAAGAAGA TTTGTTGAGC GCAGCATGTT GTAGGCGGCA TCTCAACTCC
    AAAACGTGTA AAGCATTAAG CGTCTATTCT GCAGCATACT CCTTAAGCCA GAAACTGAAA
    CTAGGTAACA GCGGCAACGT TATTTTGAAG GACATGAAGA GGTTCTACAG CGTAGATGCC
    CACGGCGTCC TTAAAAAGAC ATCCTCTTGG TTGGATGACC CCAGAAGACA TTCCATAGAA
    ATTTGTTCTT TAGAGAACAC TCTTCATCAC TACTCTACTT CACGTTCTTG TGGATTCATT
    AGCCACGTAT TCCAGAAGAA GGAGGACTTG CAAATAGTGC AGAGGACGGG CGGACAGGAT
    CCCGTCTGTC CAGATTGTGT TCACTCAGGA GAAGCATCAG AACTACCACT CTCTTACGTC
    TCGAGTGGGC AATCACAATC GACAGACTCG ACCGAGCTCG GTAAAATAGA CTCTTTCGTT
    CCGGAAATGC AGACATCCCC TAAACCACTG GGAGATTTAG TTTCTGTCGA AAGTTTTTCT
    ATGGATGCAG TTGAATTAAA TCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750732.1
    CDS2032..9078
    Translation

    Target ORF information:

    RefSeq Version XM_031894872.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894872.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATCGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCCTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AGTAAGGAGA GAGAGCTGGC CGATCTGATG CTGGATGATG TAATATTGGA GCACTCAGCC
    AGTCCAGTTA AAGAGGCCCA GTGCAAACCC TACTACTCGG AGTATTCTCA CCCTCGGCTG
    CTCATCCATC AAATGTGCAC AAGCCATTAT CTGGATTTGT TTATTACTGG GGTCATCGGA
    TTGAATGTTA TCACTATGGC GATGGAGCAC TACCAGCAAC CCAAGGTATT GGATGAAGCT
    CTAAAAATCT GTAATTACAT ATTTACCCTC ATTTTTGTGC TGGAATCTGG GTGCAAAATG
    GTAGCTTTTG GCTTCCGGCG ATTCTTCAAA GACAGGTGGA ACCAGTTGGA TTTGGCCATT
    GTTCTGCTCT CCATTATGGG GATCACCCTG GAGGAGATTG AAGTCAATGC ATCGCTGCCC
    ATTAACCCAA CAATCATCAG AATAATGAGG GTCCTGCGGA TCGCTCGCGT GCTGAAGTTA
    TTAAAGATGG CAGTCGGTAT GCGAGCCCTG CTGGATACAG TATTGCAAGC GCTTCCTCAA
    GTGGGGAATC TCGGCCTGCT CTTTATGCTG CTGTTTTTCA TATTTGCCGC TTTGGGAGTT
    GAACTTTTTG GCGACTTAGA ATGCACTGAT TCCCACCCGT GTGAAGGTCT CGGCAGGCAC
    GCCACGTTCA GGAACTTCGG CATGGCCTTT CTGACTTTGT TCAGAGTATC CACAGGAGAT
    AACTGGAACG GTATAATGAA GGACACACTC CGTGATTGCA ACGAGGATTC CACCTGTTAC
    AACACAGTCA TCTCCCCCAT CTATTTTGTC TCTTTTGTGC TGACTGCCCA GTTTGTCCTG
    GTCAATGTGG TAATAGCGGT GCTCATGAAA CACCTTGAGG AAAGCAACAA GGAAGCTAAG
    GAGGAGGCGG AGCTGGAGGC AGAGATGGAA CTAGAGTTTC AGATCCAGAA CATGCAGGTT
    CCGGATCCTG ACCCATTTAT GTGGGCTGGG AGAGATCTCG GCTATCCCCA GGAAGGTTCT
    TACGGATATA TGAACTCCGT GCAGGACAAG GCCGACTCAC AGCTTTCATT AGTCAATCCC
    ATGGGATCTT TGGAAGGAGA GATGAAGCTG ATGGACAATT TGTCTGGCTC TGTGTGCCAC
    CATTACGCCC TCCCTCCCTC TGACAAGGGC TCCTCGGAAA AACAGATCCC ATTGGCAGAG
    ATGGAAGGCC TATCCATGAC ATCAGACATT CTGTCCGAGA AATCATGGTC CTTAGCTCTG
    ACGGACGACT CCCTGCCAGA TGACCCTAAT CCCAACCCAT TTGGTGTACT GGAAACCAAA
    CTTGGCCTCC CCGAGGAGAA ACATTCGGCA ACCGTTGAGG AGGCTACGAA TCTTCTCAGC
    GTCCGGAAAC CGTCAGTATG CCGCACACAT TCCCTGCCCA ATGACAGCTA CATGTTGCAA
    ACGGGAAGCA CGTGCACTGA GTGCCAGGAG CAAAGAAACC ACTGTTACCA GAAATCAGGT
    TCCAGCTCAA CGGTTCAGTC TCAGCCAAAT GACCTCAACT CTCTTCTCCA AGTGCCACAA
    ATCTCCATCC CTTATGAAGC TTCAGGAAGT ACAGTGATAT CAGAGATTCC CAAACTCCCC
    GCCCCCTTGC GCTCTCCATC GGCTAATAGG ATGCTCCGCA GACAGGAAGC AATCAGAACC
    GACTCTTTGG AATTTTTTCC CGATAGCAAA GAAAATGCTG GCATAACTGA GCCCCCACCT
    GTATATATTC CAATGTGCCA GGTGCCCACC GAGACACCCT CGGCTTTTAT AGAACCAGAA
    AGAACTTTAT GCCAGTTGAA GCGACGCAGT GTTTACACCC AGCAAGTCCA AGATACCTGC
    AAGATGTTTA GGTACCCTGA TGCTTCTCAA ACCAACATGG ACACGTTACG AGAAACCTCA
    AAGATTCCAG AGAAAGAAGA TTTGTTGAGC GCAGCATGTT GTAGGCGGCA TCTCAACTCC
    AAAACGTGTA AAGCATTAAG CGTCTATTCT GCAGCATACT CCTTAAGCCA GAAACTGAAA
    CTAGGTAACA GCGGCAACGT TATTTTGAAG GACATGAAGA GGTTCTACAG CGTAGATGCC
    CACGGCGTCC TTAAAAAGAC ATCCTCTTGG TTGGATGACC CCAGAAGACA TTCCATAGAA
    ATTTGTTCTT TAGAGAACAC TCTTCATCAC TACTCTACTT CACGTTCTTG TGGATTCATT
    AGCCACGTAT TCCAGAAGAA GGAGGACTTG CAAATAGTGC AGAGGACGGG CGGACAGGAT
    CCCGTCTGTC CAGATTGTGT TCACTCAGGA GAAGCATCAG AACTACCACT CTCTTACGTC
    TCGAGTGGGC AATCACAATC GACAGACTCG ACCGAGCTCG GTAAAATAGA CTCTTTCGTT
    CCGGAAATGC AGACATCCCC TAAACCACTG GGAGATTTAG TTTCTGTCGA AAGTTTTTCT
    ATGGATGCAG TTGAATTAAA TCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51014
    Clone ID Related Accession (Same CDS sequence) XM_004918488.3
    Accession Version XM_004918488.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7035bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004918488.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATCGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCCTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AGTAAGGAGA GAGAGCTGGC CGAGGCCCAG TGCAAACCCT ACTACTCGGA GTATTCTCAC
    CCTCGGCTGC TCATCCATCA AATGTGCACA AGCCATTATC TGGATTTGTT TATTACTGGG
    GTCATCGGAT TGAATGTTAT CACTATGGCG ATGGAGCACT ACCAGCAACC CAAGGTATTG
    GATGAAGCTC TAAAAATCTG TAATTACATA TTTACCCTCA TTTTTGTGCT GGAATCTGGG
    TGCAAAATGG TAGCTTTTGG CTTCCGGCGA TTCTTCAAAG ACAGGTGGAA CCAGTTGGAT
    TTGGCCATTG TTCTGCTCTC CATTATGGGG ATCACCCTGG AGGAGATTGA AGTCAATGCA
    TCGCTGCCCA TTAACCCAAC AATCATCAGA ATAATGAGGG TCCTGCGGAT CGCTCGCGTG
    CTGAAGTTAT TAAAGATGGC AGTCGGTATG CGAGCCCTGC TGGATACAGT ATTGCAAGCG
    CTTCCTCAAG TGGGGAATCT CGGCCTGCTC TTTATGCTGC TGTTTTTCAT ATTTGCCGCT
    TTGGGAGTTG AACTTTTTGG CGACTTAGAA TGCACTGATT CCCACCCGTG TGAAGGTCTC
    GGCAGGCACG CCACGTTCAG GAACTTCGGC ATGGCCTTTC TGACTTTGTT CAGAGTATCC
    ACAGGAGATA ACTGGAACGG TATAATGAAG GACACACTCC GTGATTGCAA CGAGGATTCC
    ACCTGTTACA ACACAGTCAT CTCCCCCATC TATTTTGTCT CTTTTGTGCT GACTGCCCAG
    TTTGTCCTGG TCAATGTGGT AATAGCGGTG CTCATGAAAC ACCTTGAGGA AAGCAACAAG
    GAAGCTAAGG AGGAGGCGGA GCTGGAGGCA GAGATGGAAC TAGAGTTTCA GATCCAGAAC
    ATGCAGGTTC CGGATCCTGA CCCATTTATG TGGGCTGGGA GAGATCTCGG CTATCCCCAG
    GAAGGTTCTT ACGGATATAT GAACTCCGTG CAGGACAAGG CCGACTCACA GCTTTCATTA
    GTCAATCCCA TGGAAAGACA CATGTTTGAT ACAATATCAC TACTGATCCA GGGATCTTTG
    GAAGGAGAGA TGAAGCTGAT GGACAATTTG TCTGGCTCTG TGTGCCACCA TTACGCCCTC
    CCTCCCTCTG ACAAGGGCTC CTCGGAAAAA CAGATCCCAT TGGCAGAGAT GGAAGGCCTA
    TCCATGACAT CAGACATTCT GTCCGAGAAA TCATGGTCCT TAGCTCTGAC GGACGACTCC
    CTGCCAGATG ACCCTAATCC CAACCCATTT GGTGTACTGG AAACCAAACT TGGCCTCCCC
    GAGGAGAAAC ATTCGGCAAC CGTTGAGGAG GCTACGAATC TTCTCAGCGT CCGGAAACCG
    TCAGTATGCC GCACACATTC CCTGCCCAAT GACAGCTACA TGTTGCAAAC GGGAAGCACG
    TGCACTGAGT GCCAGGAGCA AAGAAACCAC TGTTACCAGA AATCAGGTTC CAGCTCAACG
    GTTCAGTCTC AGCCAAATGA CCTCAACTCT CTTCTCCAAG TGCCACAAAT CTCCATCCCT
    TATGAAGCTT CAGGAAGTAC AGTGATATCA GAGATTCCCA AACTCCCCGC CCCCTTGCGC
    TCTCCATCGG CTAATAGGAT GCTCCGCAGA CAGGAAGCAA TCAGAACCGA CTCTTTGGAA
    TTTTTTCCCG ATAGCAAAGA AAATGCTGGC ATAACTGAGC CCCCACCTGT ATATATTCCA
    ATGTGCCAGG TGCCCACCGA GACACCCTCG GCTTTTATAG AACCAGAAAG AACTTTATGC
    CAGTTGAAGC GACGCAGTGT TTACACCCAG CAAGTCCAAG ATACCTGCAA GATGTTTAGG
    TACCCTGATG CTTCTCAAAC CAACATGGAC ACGTTACGAG AAACCTCAAA GATTCCAGAG
    AAAGAAGATT TGTTGAGCGC AGCATGTTGT AGGCGGCATC TCAACTCCAA AACGTGTAAA
    GCATTAAGCG TCTATTCTGC AGCATACTCC TTAAGCCAGA AACTGAAACT AGGTAACAGC
    GGCAACGTTA TTTTGAAGGA CATGAAGAGG TTCTACAGCG TAGATGCCCA CGGCGTCCTT
    AAAAAGACAT CCTCTTGGTT GGATGACCCC AGAAGACATT CCATAGAAAT TTGTTCTTTA
    GAGAACACTC TTCATCACTA CTCTACTTCA CGTTCTTGTG GATTCATTAG CCACGTATTC
    CAGAAGAAGG AGGACTTGCA AATAGTGCAG AGGACGGGCG GACAGGATCC CGTCTGTCCA
    GATTGTGTTC ACTCAGGAGA AGCATCAGAA CTACCACTCT CTTACGTCTC GAGTGGGCAA
    TCACAATCGA CAGACTCGAC CGAGCTCGGT AAAATAGACT CTTTCGTTCC GGAAATGCAG
    ACATCCCCTA AACCACTGGG AGATTTAGTT TCTGTCGAAA GTTTTTCTAT GGATGCAGTT
    GAATTAAATC AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004918545.2
    CDS2032..9066
    Translation

    Target ORF information:

    RefSeq Version XM_004918488.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004918488.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATCGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCCTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AGTAAGGAGA GAGAGCTGGC CGAGGCCCAG TGCAAACCCT ACTACTCGGA GTATTCTCAC
    CCTCGGCTGC TCATCCATCA AATGTGCACA AGCCATTATC TGGATTTGTT TATTACTGGG
    GTCATCGGAT TGAATGTTAT CACTATGGCG ATGGAGCACT ACCAGCAACC CAAGGTATTG
    GATGAAGCTC TAAAAATCTG TAATTACATA TTTACCCTCA TTTTTGTGCT GGAATCTGGG
    TGCAAAATGG TAGCTTTTGG CTTCCGGCGA TTCTTCAAAG ACAGGTGGAA CCAGTTGGAT
    TTGGCCATTG TTCTGCTCTC CATTATGGGG ATCACCCTGG AGGAGATTGA AGTCAATGCA
    TCGCTGCCCA TTAACCCAAC AATCATCAGA ATAATGAGGG TCCTGCGGAT CGCTCGCGTG
    CTGAAGTTAT TAAAGATGGC AGTCGGTATG CGAGCCCTGC TGGATACAGT ATTGCAAGCG
    CTTCCTCAAG TGGGGAATCT CGGCCTGCTC TTTATGCTGC TGTTTTTCAT ATTTGCCGCT
    TTGGGAGTTG AACTTTTTGG CGACTTAGAA TGCACTGATT CCCACCCGTG TGAAGGTCTC
    GGCAGGCACG CCACGTTCAG GAACTTCGGC ATGGCCTTTC TGACTTTGTT CAGAGTATCC
    ACAGGAGATA ACTGGAACGG TATAATGAAG GACACACTCC GTGATTGCAA CGAGGATTCC
    ACCTGTTACA ACACAGTCAT CTCCCCCATC TATTTTGTCT CTTTTGTGCT GACTGCCCAG
    TTTGTCCTGG TCAATGTGGT AATAGCGGTG CTCATGAAAC ACCTTGAGGA AAGCAACAAG
    GAAGCTAAGG AGGAGGCGGA GCTGGAGGCA GAGATGGAAC TAGAGTTTCA GATCCAGAAC
    ATGCAGGTTC CGGATCCTGA CCCATTTATG TGGGCTGGGA GAGATCTCGG CTATCCCCAG
    GAAGGTTCTT ACGGATATAT GAACTCCGTG CAGGACAAGG CCGACTCACA GCTTTCATTA
    GTCAATCCCA TGGAAAGACA CATGTTTGAT ACAATATCAC TACTGATCCA GGGATCTTTG
    GAAGGAGAGA TGAAGCTGAT GGACAATTTG TCTGGCTCTG TGTGCCACCA TTACGCCCTC
    CCTCCCTCTG ACAAGGGCTC CTCGGAAAAA CAGATCCCAT TGGCAGAGAT GGAAGGCCTA
    TCCATGACAT CAGACATTCT GTCCGAGAAA TCATGGTCCT TAGCTCTGAC GGACGACTCC
    CTGCCAGATG ACCCTAATCC CAACCCATTT GGTGTACTGG AAACCAAACT TGGCCTCCCC
    GAGGAGAAAC ATTCGGCAAC CGTTGAGGAG GCTACGAATC TTCTCAGCGT CCGGAAACCG
    TCAGTATGCC GCACACATTC CCTGCCCAAT GACAGCTACA TGTTGCAAAC GGGAAGCACG
    TGCACTGAGT GCCAGGAGCA AAGAAACCAC TGTTACCAGA AATCAGGTTC CAGCTCAACG
    GTTCAGTCTC AGCCAAATGA CCTCAACTCT CTTCTCCAAG TGCCACAAAT CTCCATCCCT
    TATGAAGCTT CAGGAAGTAC AGTGATATCA GAGATTCCCA AACTCCCCGC CCCCTTGCGC
    TCTCCATCGG CTAATAGGAT GCTCCGCAGA CAGGAAGCAA TCAGAACCGA CTCTTTGGAA
    TTTTTTCCCG ATAGCAAAGA AAATGCTGGC ATAACTGAGC CCCCACCTGT ATATATTCCA
    ATGTGCCAGG TGCCCACCGA GACACCCTCG GCTTTTATAG AACCAGAAAG AACTTTATGC
    CAGTTGAAGC GACGCAGTGT TTACACCCAG CAAGTCCAAG ATACCTGCAA GATGTTTAGG
    TACCCTGATG CTTCTCAAAC CAACATGGAC ACGTTACGAG AAACCTCAAA GATTCCAGAG
    AAAGAAGATT TGTTGAGCGC AGCATGTTGT AGGCGGCATC TCAACTCCAA AACGTGTAAA
    GCATTAAGCG TCTATTCTGC AGCATACTCC TTAAGCCAGA AACTGAAACT AGGTAACAGC
    GGCAACGTTA TTTTGAAGGA CATGAAGAGG TTCTACAGCG TAGATGCCCA CGGCGTCCTT
    AAAAAGACAT CCTCTTGGTT GGATGACCCC AGAAGACATT CCATAGAAAT TTGTTCTTTA
    GAGAACACTC TTCATCACTA CTCTACTTCA CGTTCTTGTG GATTCATTAG CCACGTATTC
    CAGAAGAAGG AGGACTTGCA AATAGTGCAG AGGACGGGCG GACAGGATCC CGTCTGTCCA
    GATTGTGTTC ACTCAGGAGA AGCATCAGAA CTACCACTCT CTTACGTCTC GAGTGGGCAA
    TCACAATCGA CAGACTCGAC CGAGCTCGGT AAAATAGACT CTTTCGTTCC GGAAATGCAG
    ACATCCCCTA AACCACTGGG AGATTTAGTT TCTGTCGAAA GTTTTTCTAT GGATGCAGTT
    GAATTAAATC AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51015
    Clone ID Related Accession (Same CDS sequence) XM_031894873.1
    Accession Version XM_031894873.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7020bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TCAGCCACAC CCATGCCCAT GCCAAAATGT GCTTTGTTTG GAGATATCCC CATGGCTGGT
    GAATCTAGGA GGGAGAGTAA TGTCTCCGGT GGCCCCCATG CTTGTGAAAT CAGGTCTATG
    TCAAGTTCAA GGAACTCCCC GCACAGCTCC TGGGGTGCAG TGTGTAGTTG GAATAGCCGT
    AGATCTAGTT GGAACAGCAT CGGACGGGCT TCCAGCCTAA AACGGCACGA GCAAAGCGGT
    GAACACAAAT CCTTGCTGTC TGCTGATGGA AAAGAAAGCT CAGAAGGCGA CACATCAGAC
    GAGGAGGTGT CCAGCCGTAC CGGCTCTTCC CTCAACCGGA TGGACCTCGT GGGTAAACGG
    GAGGCGTACG ACATGCACGA TACATTGCAT GTGCCTTATT CTTATGGAAT GCAGAGCAGA
    CACAGCCTGC CTGGGGAGTA CCAAGACTGC AATGGAAAGA CATCACCTGT TTTTCCTCCA
    CTCCATGTAG AAGAATCCAA GGTTGACTAT AGCAATAACA TCGATGATGA TGTAAACATG
    AGTAGGGGAG AAAGACTGAA AGCTTGGATC ATATCCCATT TACCAGCCTG GTGCAAGGAG
    AGAGAAACTT GGTCGCTGTA TTTGTTCCCG CCATATTCCA AATTCCGGCT GATGTGCAAT
    AAAATTATCA CCCACAGAAT GTTTGATCAC GTGGTCTTGG TCATCATATT TCTCAACTGT
    ATAACCATTG CCATGGAAAG GCCCAAAATA GACCCCCACA GTGCCGAAAG AATATTTCTC
    ACGTTGTCCA ACTACATATT CACCTTGATT TTCTTGGCTG AAATGACAGT GAAGGTGGTG
    GCCATGAACT TTTGCTTTGG TGGGAAGGCG TACCTGAGGA GCAGTTGGAA TGTGTTGGAT
    GGAATGCTGG TCTTGATCTC AGTCATTGAC ATTCTAGTCT CTATGGTGTC CGACAGCGGC
    ACCAAGATCC TTGGCATGCT AAGAGTGCTG CGGCTGCTCC GGACTTTAAG GCCACTCAGG
    GTGATCAGTC GAGCTCAGGG ACTGAAGCTG GTGGTTGAGA CACTAATGTC TTCTTTAAAG
    CCTATCGGTA ACATTGTGGT GATCTGCTGT GCCTTCTTCA TCATATTTGG GATACTTGGA
    GTTCAGCTTT TCAAGGGGAA ATTTTATTAC TGCCAGGGAG AGGACACTCG AAACATCACT
    AATAAATCCG ACTGCCTTGA AAACAAGTAT AAATGGGCAA GACACAAGTA CAACTTTGAT
    AATCTTGGCC AGGCTCTGAT GTCTCTGTTT GTCCTGGCAT CTAAAGACGG ATGGGTGGAC
    ATCATGTACG ATGGGCTGGA TGCTGTCGGG GTTGACCAGC AGCCAGTGAT GAACTACAAC
    CCTTGGATGC TGCTCTACTT CATCTCGTTT CTGCTGATCG TGGCGTTCTT TGTGCTCAAC
    ATGTTTGTGG GCGTGGTGGT GGAGAACTTC CATAAGTGCC GGCAGCATCA GGAGGAGGAG
    GAGGCGAAGA GACGGGAAGA GAAGAGGCTA CGCAGGATGG AGAAAAAGAG AAGGAGTAAG
    GAGAGAGAGC TGGCCGATCT GATGCTGGAT GATGTAATAT TGGAGCACTC AGCCAGTCCA
    GTTAAAGAGG CCCAGTGCAA ACCCTACTAC TCGGAGTATT CTCACCCTCG GCTGCTCATC
    CATCAAATGT GCACAAGCCA TTATCTGGAT TTGTTTATTA CTGGGGTCAT CGGATTGAAT
    GTTATCACTA TGGCGATGGA GCACTACCAG CAACCCAAGG TATTGGATGA AGCTCTAAAA
    ATCTGTAATT ACATATTTAC CCTCATTTTT GTGCTGGAAT CTGGGTGCAA AATGGTAGCT
    TTTGGCTTCC GGCGATTCTT CAAAGACAGG TGGAACCAGT TGGATTTGGC CATTGTTCTG
    CTCTCCATTA TGGGGATCAC CCTGGAGGAG ATTGAAGTCA ATGCATCGCT GCCCATTAAC
    CCAACAATCA TCAGAATAAT GAGGGTCCTG CGGATCGCTC GCGTGCTGAA GTTATTAAAG
    ATGGCAGTCG GTATGCGAGC CCTGCTGGAT ACAGTATTGC AAGCGCTTCC TCAAGTGGGG
    AATCTCGGCC TGCTCTTTAT GCTGCTGTTT TTCATATTTG CCGCTTTGGG AGTTGAACTT
    TTTGGCGACT TAGAATGCAC TGATTCCCAC CCGTGTGAAG GTCTCGGCAG GCACGCCACG
    TTCAGGAACT TCGGCATGGC CTTTCTGACT TTGTTCAGAG TATCCACAGG AGATAACTGG
    AACGGTATAA TGAAGGACAC ACTCCGTGAT TGCAACGAGG ATTCCACCTG TTACAACACA
    GTCATCTCCC CCATCTATTT TGTCTCTTTT GTGCTGACTG CCCAGTTTGT CCTGGTCAAT
    GTGGTAATAG CGGTGCTCAT GAAACACCTT GAGGAAAGCA ACAAGGAAGC TAAGGAGGAG
    GCGGAGCTGG AGGCAGAGAT GGAACTAGAG TTTCAGATCC AGAACATGCA GGTTCCGGAT
    CCTGACCCAT TTATGTGGGC TGGGAGAGAT CTCGGCTATC CCCAGGAAGG TTCTTACGGA
    TATATGAACT CCGTGCAGGA CAAGGCCGAC TCACAGCTTT CATTAGTCAA TCCCATGGAA
    AGACACATGT TTGATACAAT ATCACTACTG ATCCAGGGAT CTTTGGAAGG AGAGATGAAG
    CTGATGGACA ATTTGTCTGG CTCTGTGTGC CACCATTACG CCCTCCCTCC CTCTGACAAG
    GGCTCCTCGG AAAAACAGAT CCCATTGGCA GAGATGGAAG GCCTATCCAT GACATCAGAC
    ATTCTGTCCG AGAAATCATG GTCCTTAGCT CTGACGGACG ACTCCCTGCC AGATGACCCT
    AATCCCAACC CATTTGGTGT ACTGGAAACC AAACTTGGCC TCCCCGAGGA GAAACATTCG
    GCAACCGTTG AGGAGGCTAC GAATCTTCTC AGCGTCCGGA AACCGTCAGT ATGCCGCACA
    CATTCCCTGC CCAATGACAG CTACATGTTG CAAACGGGAA GCACGTGCAC TGAGTGCCAG
    GAGCAAAGAA ACCACTGTTA CCAGAAATCA GGTTCCAGCT CAACGGTTCA GTCTCAGCCA
    AATGACCTCA ACTCTCTTCT CCAAGTGCCA CAAATCTCCA TCCCTTATGA AGCTTCAGGA
    AGTACAGTGA TATCAGAGAT TCCCAAACTC CCCGCCCCCT TGCGCTCTCC ATCGGCTAAT
    AGGATGCTCC GCAGACAGGA AGCAATCAGA ACCGACTCTT TGGAATTTTT TCCCGATAGC
    AAAGAAAATG CTGGCATAAC TGAGCCCCCA CCTGTATATA TTCCAATGTG CCAGGTGCCC
    ACCGAGACAC CCTCGGCTTT TATAGAACCA GAAAGAACTT TATGCCAGTT GAAGCGACGC
    AGTGTTTACA CCCAGCAAGT CCAAGATACC TGCAAGATGT TTAGGTACCC TGATGCTTCT
    CAAACCAACA TGGACACGTT ACGAGAAACC TCAAAGATTC CAGAGAAAGA AGATTTGTTG
    AGCGCAGCAT GTTGTAGGCG GCATCTCAAC TCCAAAACGT GTAAAGCATT AAGCGTCTAT
    TCTGCAGCAT ACTCCTTAAG CCAGAAACTG AAACTAGGTA ACAGCGGCAA CGTTATTTTG
    AAGGACATGA AGAGGTTCTA CAGCGTAGAT GCCCACGGCG TCCTTAAAAA GACATCCTCT
    TGGTTGGATG ACCCCAGAAG ACATTCCATA GAAATTTGTT CTTTAGAGAA CACTCTTCAT
    CACTACTCTA CTTCACGTTC TTGTGGATTC ATTAGCCACG TATTCCAGAA GAAGGAGGAC
    TTGCAAATAG TGCAGAGGAC GGGCGGACAG GATCCCGTCT GTCCAGATTG TGTTCACTCA
    GGAGAAGCAT CAGAACTACC ACTCTCTTAC GTCTCGAGTG GGCAATCACA ATCGACAGAC
    TCGACCGAGC TCGGTAAAAT AGACTCTTTC GTTCCGGAAA TGCAGACATC CCCTAAACCA
    CTGGGAGATT TAGTTTCTGT CGAAAGTTTT TCTATGGATG CAGTTGAATT AAATCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750733.1
    CDS2032..9051
    Translation

    Target ORF information:

    RefSeq Version XM_031894873.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894873.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TCAGCCACAC CCATGCCCAT GCCAAAATGT GCTTTGTTTG GAGATATCCC CATGGCTGGT
    GAATCTAGGA GGGAGAGTAA TGTCTCCGGT GGCCCCCATG CTTGTGAAAT CAGGTCTATG
    TCAAGTTCAA GGAACTCCCC GCACAGCTCC TGGGGTGCAG TGTGTAGTTG GAATAGCCGT
    AGATCTAGTT GGAACAGCAT CGGACGGGCT TCCAGCCTAA AACGGCACGA GCAAAGCGGT
    GAACACAAAT CCTTGCTGTC TGCTGATGGA AAAGAAAGCT CAGAAGGCGA CACATCAGAC
    GAGGAGGTGT CCAGCCGTAC CGGCTCTTCC CTCAACCGGA TGGACCTCGT GGGTAAACGG
    GAGGCGTACG ACATGCACGA TACATTGCAT GTGCCTTATT CTTATGGAAT GCAGAGCAGA
    CACAGCCTGC CTGGGGAGTA CCAAGACTGC AATGGAAAGA CATCACCTGT TTTTCCTCCA
    CTCCATGTAG AAGAATCCAA GGTTGACTAT AGCAATAACA TCGATGATGA TGTAAACATG
    AGTAGGGGAG AAAGACTGAA AGCTTGGATC ATATCCCATT TACCAGCCTG GTGCAAGGAG
    AGAGAAACTT GGTCGCTGTA TTTGTTCCCG CCATATTCCA AATTCCGGCT GATGTGCAAT
    AAAATTATCA CCCACAGAAT GTTTGATCAC GTGGTCTTGG TCATCATATT TCTCAACTGT
    ATAACCATTG CCATGGAAAG GCCCAAAATA GACCCCCACA GTGCCGAAAG AATATTTCTC
    ACGTTGTCCA ACTACATATT CACCTTGATT TTCTTGGCTG AAATGACAGT GAAGGTGGTG
    GCCATGAACT TTTGCTTTGG TGGGAAGGCG TACCTGAGGA GCAGTTGGAA TGTGTTGGAT
    GGAATGCTGG TCTTGATCTC AGTCATTGAC ATTCTAGTCT CTATGGTGTC CGACAGCGGC
    ACCAAGATCC TTGGCATGCT AAGAGTGCTG CGGCTGCTCC GGACTTTAAG GCCACTCAGG
    GTGATCAGTC GAGCTCAGGG ACTGAAGCTG GTGGTTGAGA CACTAATGTC TTCTTTAAAG
    CCTATCGGTA ACATTGTGGT GATCTGCTGT GCCTTCTTCA TCATATTTGG GATACTTGGA
    GTTCAGCTTT TCAAGGGGAA ATTTTATTAC TGCCAGGGAG AGGACACTCG AAACATCACT
    AATAAATCCG ACTGCCTTGA AAACAAGTAT AAATGGGCAA GACACAAGTA CAACTTTGAT
    AATCTTGGCC AGGCTCTGAT GTCTCTGTTT GTCCTGGCAT CTAAAGACGG ATGGGTGGAC
    ATCATGTACG ATGGGCTGGA TGCTGTCGGG GTTGACCAGC AGCCAGTGAT GAACTACAAC
    CCTTGGATGC TGCTCTACTT CATCTCGTTT CTGCTGATCG TGGCGTTCTT TGTGCTCAAC
    ATGTTTGTGG GCGTGGTGGT GGAGAACTTC CATAAGTGCC GGCAGCATCA GGAGGAGGAG
    GAGGCGAAGA GACGGGAAGA GAAGAGGCTA CGCAGGATGG AGAAAAAGAG AAGGAGTAAG
    GAGAGAGAGC TGGCCGATCT GATGCTGGAT GATGTAATAT TGGAGCACTC AGCCAGTCCA
    GTTAAAGAGG CCCAGTGCAA ACCCTACTAC TCGGAGTATT CTCACCCTCG GCTGCTCATC
    CATCAAATGT GCACAAGCCA TTATCTGGAT TTGTTTATTA CTGGGGTCAT CGGATTGAAT
    GTTATCACTA TGGCGATGGA GCACTACCAG CAACCCAAGG TATTGGATGA AGCTCTAAAA
    ATCTGTAATT ACATATTTAC CCTCATTTTT GTGCTGGAAT CTGGGTGCAA AATGGTAGCT
    TTTGGCTTCC GGCGATTCTT CAAAGACAGG TGGAACCAGT TGGATTTGGC CATTGTTCTG
    CTCTCCATTA TGGGGATCAC CCTGGAGGAG ATTGAAGTCA ATGCATCGCT GCCCATTAAC
    CCAACAATCA TCAGAATAAT GAGGGTCCTG CGGATCGCTC GCGTGCTGAA GTTATTAAAG
    ATGGCAGTCG GTATGCGAGC CCTGCTGGAT ACAGTATTGC AAGCGCTTCC TCAAGTGGGG
    AATCTCGGCC TGCTCTTTAT GCTGCTGTTT TTCATATTTG CCGCTTTGGG AGTTGAACTT
    TTTGGCGACT TAGAATGCAC TGATTCCCAC CCGTGTGAAG GTCTCGGCAG GCACGCCACG
    TTCAGGAACT TCGGCATGGC CTTTCTGACT TTGTTCAGAG TATCCACAGG AGATAACTGG
    AACGGTATAA TGAAGGACAC ACTCCGTGAT TGCAACGAGG ATTCCACCTG TTACAACACA
    GTCATCTCCC CCATCTATTT TGTCTCTTTT GTGCTGACTG CCCAGTTTGT CCTGGTCAAT
    GTGGTAATAG CGGTGCTCAT GAAACACCTT GAGGAAAGCA ACAAGGAAGC TAAGGAGGAG
    GCGGAGCTGG AGGCAGAGAT GGAACTAGAG TTTCAGATCC AGAACATGCA GGTTCCGGAT
    CCTGACCCAT TTATGTGGGC TGGGAGAGAT CTCGGCTATC CCCAGGAAGG TTCTTACGGA
    TATATGAACT CCGTGCAGGA CAAGGCCGAC TCACAGCTTT CATTAGTCAA TCCCATGGAA
    AGACACATGT TTGATACAAT ATCACTACTG ATCCAGGGAT CTTTGGAAGG AGAGATGAAG
    CTGATGGACA ATTTGTCTGG CTCTGTGTGC CACCATTACG CCCTCCCTCC CTCTGACAAG
    GGCTCCTCGG AAAAACAGAT CCCATTGGCA GAGATGGAAG GCCTATCCAT GACATCAGAC
    ATTCTGTCCG AGAAATCATG GTCCTTAGCT CTGACGGACG ACTCCCTGCC AGATGACCCT
    AATCCCAACC CATTTGGTGT ACTGGAAACC AAACTTGGCC TCCCCGAGGA GAAACATTCG
    GCAACCGTTG AGGAGGCTAC GAATCTTCTC AGCGTCCGGA AACCGTCAGT ATGCCGCACA
    CATTCCCTGC CCAATGACAG CTACATGTTG CAAACGGGAA GCACGTGCAC TGAGTGCCAG
    GAGCAAAGAA ACCACTGTTA CCAGAAATCA GGTTCCAGCT CAACGGTTCA GTCTCAGCCA
    AATGACCTCA ACTCTCTTCT CCAAGTGCCA CAAATCTCCA TCCCTTATGA AGCTTCAGGA
    AGTACAGTGA TATCAGAGAT TCCCAAACTC CCCGCCCCCT TGCGCTCTCC ATCGGCTAAT
    AGGATGCTCC GCAGACAGGA AGCAATCAGA ACCGACTCTT TGGAATTTTT TCCCGATAGC
    AAAGAAAATG CTGGCATAAC TGAGCCCCCA CCTGTATATA TTCCAATGTG CCAGGTGCCC
    ACCGAGACAC CCTCGGCTTT TATAGAACCA GAAAGAACTT TATGCCAGTT GAAGCGACGC
    AGTGTTTACA CCCAGCAAGT CCAAGATACC TGCAAGATGT TTAGGTACCC TGATGCTTCT
    CAAACCAACA TGGACACGTT ACGAGAAACC TCAAAGATTC CAGAGAAAGA AGATTTGTTG
    AGCGCAGCAT GTTGTAGGCG GCATCTCAAC TCCAAAACGT GTAAAGCATT AAGCGTCTAT
    TCTGCAGCAT ACTCCTTAAG CCAGAAACTG AAACTAGGTA ACAGCGGCAA CGTTATTTTG
    AAGGACATGA AGAGGTTCTA CAGCGTAGAT GCCCACGGCG TCCTTAAAAA GACATCCTCT
    TGGTTGGATG ACCCCAGAAG ACATTCCATA GAAATTTGTT CTTTAGAGAA CACTCTTCAT
    CACTACTCTA CTTCACGTTC TTGTGGATTC ATTAGCCACG TATTCCAGAA GAAGGAGGAC
    TTGCAAATAG TGCAGAGGAC GGGCGGACAG GATCCCGTCT GTCCAGATTG TGTTCACTCA
    GGAGAAGCAT CAGAACTACC ACTCTCTTAC GTCTCGAGTG GGCAATCACA ATCGACAGAC
    TCGACCGAGC TCGGTAAAAT AGACTCTTTC GTTCCGGAAA TGCAGACATC CCCTAAACCA
    CTGGGAGATT TAGTTTCTGT CGAAAGTTTT TCTATGGATG CAGTTGAATT AAATCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51016
    Clone ID Related Accession (Same CDS sequence) XM_004918490.3
    Accession Version XM_004918490.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004918490.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATCGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCCTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AAGGCCCAGT GCAAACCCTA CTACTCGGAG TATTCTCACC CTCGGCTGCT CATCCATCAA
    ATGTGCACAA GCCATTATCT GGATTTGTTT ATTACTGGGG TCATCGGATT GAATGTTATC
    ACTATGGCGA TGGAGCACTA CCAGCAACCC AAGGTATTGG ATGAAGCTCT AAAAATCTGT
    AATTACATAT TTACCCTCAT TTTTGTGCTG GAATCTGGGT GCAAAATGGT AGCTTTTGGC
    TTCCGGCGAT TCTTCAAAGA CAGGTGGAAC CAGTTGGATT TGGCCATTGT TCTGCTCTCC
    ATTATGGGGA TCACCCTGGA GGAGATTGAA GTCAATGCAT CGCTGCCCAT TAACCCAACA
    ATCATCAGAA TAATGAGGGT CCTGCGGATC GCTCGCGTGC TGAAGTTATT AAAGATGGCA
    GTCGGTATGC GAGCCCTGCT GGATACAGTA TTGCAAGCGC TTCCTCAAGT GGGGAATCTC
    GGCCTGCTCT TTATGCTGCT GTTTTTCATA TTTGCCGCTT TGGGAGTTGA ACTTTTTGGC
    GACTTAGAAT GCACTGATTC CCACCCGTGT GAAGGTCTCG GCAGGCACGC CACGTTCAGG
    AACTTCGGCA TGGCCTTTCT GACTTTGTTC AGAGTATCCA CAGGAGATAA CTGGAACGGT
    ATAATGAAGG ACACACTCCG TGATTGCAAC GAGGATTCCA CCTGTTACAA CACAGTCATC
    TCCCCCATCT ATTTTGTCTC TTTTGTGCTG ACTGCCCAGT TTGTCCTGGT CAATGTGGTA
    ATAGCGGTGC TCATGAAACA CCTTGAGGAA AGCAACAAGG AAGCTAAGGA GGAGGCGGAG
    CTGGAGGCAG AGATGGAACT AGAGTTTCAG ATCCAGAACA TGCAGGTTCC GGATCCTGAC
    CCATTTATGT GGGCTGGGAG AGATCTCGGC TATCCCCAGG AAGGTTCTTA CGGATATATG
    AACTCCGTGC AGGACAAGGC CGACTCACAG CTTTCATTAG TCAATCCCAT GGAAAGACAC
    ATGTTTGATA CAATATCACT ACTGATCCAG GGATCTTTGG AAGGAGAGAT GAAGCTGATG
    GACAATTTGT CTGGCTCTGT GTGCCACCAT TACGCCCTCC CTCCCTCTGA CAAGGGCTCC
    TCGGAAAAAC AGATCCCATT GGCAGAGATG GAAGGCCTAT CCATGACATC AGACATTCTG
    TCCGAGAAAT CATGGTCCTT AGCTCTGACG GACGACTCCC TGCCAGATGA CCCTAATCCC
    AACCCATTTG GTGTACTGGA AACCAAACTT GGCCTCCCCG AGGAGAAACA TTCGGCAACC
    GTTGAGGAGG CTACGAATCT TCTCAGCGTC CGGAAACCGT CAGTATGCCG CACACATTCC
    CTGCCCAATG ACAGCTACAT GTTGCAAACG GGAAGCACGT GCACTGAGTG CCAGGAGCAA
    AGAAACCACT GTTACCAGAA ATCAGGTTCC AGCTCAACGG TTCAGTCTCA GCCAAATGAC
    CTCAACTCTC TTCTCCAAGT GCCACAAATC TCCATCCCTT ATGAAGCTTC AGGAAGTACA
    GTGATATCAG AGATTCCCAA ACTCCCCGCC CCCTTGCGCT CTCCATCGGC TAATAGGATG
    CTCCGCAGAC AGGAAGCAAT CAGAACCGAC TCTTTGGAAT TTTTTCCCGA TAGCAAAGAA
    AATGCTGGCA TAACTGAGCC CCCACCTGTA TATATTCCAA TGTGCCAGGT GCCCACCGAG
    ACACCCTCGG CTTTTATAGA ACCAGAAAGA ACTTTATGCC AGTTGAAGCG ACGCAGTGTT
    TACACCCAGC AAGTCCAAGA TACCTGCAAG ATGTTTAGGT ACCCTGATGC TTCTCAAACC
    AACATGGACA CGTTACGAGA AACCTCAAAG ATTCCAGAGA AAGAAGATTT GTTGAGCGCA
    GCATGTTGTA GGCGGCATCT CAACTCCAAA ACGTGTAAAG CATTAAGCGT CTATTCTGCA
    GCATACTCCT TAAGCCAGAA ACTGAAACTA GGTAACAGCG GCAACGTTAT TTTGAAGGAC
    ATGAAGAGGT TCTACAGCGT AGATGCCCAC GGCGTCCTTA AAAAGACATC CTCTTGGTTG
    GATGACCCCA GAAGACATTC CATAGAAATT TGTTCTTTAG AGAACACTCT TCATCACTAC
    TCTACTTCAC GTTCTTGTGG ATTCATTAGC CACGTATTCC AGAAGAAGGA GGACTTGCAA
    ATAGTGCAGA GGACGGGCGG ACAGGATCCC GTCTGTCCAG ATTGTGTTCA CTCAGGAGAA
    GCATCAGAAC TACCACTCTC TTACGTCTCG AGTGGGCAAT CACAATCGAC AGACTCGACC
    GAGCTCGGTA AAATAGACTC TTTCGTTCCG GAAATGCAGA CATCCCCTAA ACCACTGGGA
    GATTTAGTTT CTGTCGAAAG TTTTTCTATG GATGCAGTTG AATTAAATCA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004918547.2
    CDS2032..9045
    Translation

    Target ORF information:

    RefSeq Version XM_004918490.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004918490.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGATAAAT CTCATGAGCA AAGACCAGCC AAAGACCCTG GGGATCCGGG GTCGGAGCTG 
    GAAGGGCTGC CCTATCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ATGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTCCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGGGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATCGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCCTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AAGGCCCAGT GCAAACCCTA CTACTCGGAG TATTCTCACC CTCGGCTGCT CATCCATCAA
    ATGTGCACAA GCCATTATCT GGATTTGTTT ATTACTGGGG TCATCGGATT GAATGTTATC
    ACTATGGCGA TGGAGCACTA CCAGCAACCC AAGGTATTGG ATGAAGCTCT AAAAATCTGT
    AATTACATAT TTACCCTCAT TTTTGTGCTG GAATCTGGGT GCAAAATGGT AGCTTTTGGC
    TTCCGGCGAT TCTTCAAAGA CAGGTGGAAC CAGTTGGATT TGGCCATTGT TCTGCTCTCC
    ATTATGGGGA TCACCCTGGA GGAGATTGAA GTCAATGCAT CGCTGCCCAT TAACCCAACA
    ATCATCAGAA TAATGAGGGT CCTGCGGATC GCTCGCGTGC TGAAGTTATT AAAGATGGCA
    GTCGGTATGC GAGCCCTGCT GGATACAGTA TTGCAAGCGC TTCCTCAAGT GGGGAATCTC
    GGCCTGCTCT TTATGCTGCT GTTTTTCATA TTTGCCGCTT TGGGAGTTGA ACTTTTTGGC
    GACTTAGAAT GCACTGATTC CCACCCGTGT GAAGGTCTCG GCAGGCACGC CACGTTCAGG
    AACTTCGGCA TGGCCTTTCT GACTTTGTTC AGAGTATCCA CAGGAGATAA CTGGAACGGT
    ATAATGAAGG ACACACTCCG TGATTGCAAC GAGGATTCCA CCTGTTACAA CACAGTCATC
    TCCCCCATCT ATTTTGTCTC TTTTGTGCTG ACTGCCCAGT TTGTCCTGGT CAATGTGGTA
    ATAGCGGTGC TCATGAAACA CCTTGAGGAA AGCAACAAGG AAGCTAAGGA GGAGGCGGAG
    CTGGAGGCAG AGATGGAACT AGAGTTTCAG ATCCAGAACA TGCAGGTTCC GGATCCTGAC
    CCATTTATGT GGGCTGGGAG AGATCTCGGC TATCCCCAGG AAGGTTCTTA CGGATATATG
    AACTCCGTGC AGGACAAGGC CGACTCACAG CTTTCATTAG TCAATCCCAT GGAAAGACAC
    ATGTTTGATA CAATATCACT ACTGATCCAG GGATCTTTGG AAGGAGAGAT GAAGCTGATG
    GACAATTTGT CTGGCTCTGT GTGCCACCAT TACGCCCTCC CTCCCTCTGA CAAGGGCTCC
    TCGGAAAAAC AGATCCCATT GGCAGAGATG GAAGGCCTAT CCATGACATC AGACATTCTG
    TCCGAGAAAT CATGGTCCTT AGCTCTGACG GACGACTCCC TGCCAGATGA CCCTAATCCC
    AACCCATTTG GTGTACTGGA AACCAAACTT GGCCTCCCCG AGGAGAAACA TTCGGCAACC
    GTTGAGGAGG CTACGAATCT TCTCAGCGTC CGGAAACCGT CAGTATGCCG CACACATTCC
    CTGCCCAATG ACAGCTACAT GTTGCAAACG GGAAGCACGT GCACTGAGTG CCAGGAGCAA
    AGAAACCACT GTTACCAGAA ATCAGGTTCC AGCTCAACGG TTCAGTCTCA GCCAAATGAC
    CTCAACTCTC TTCTCCAAGT GCCACAAATC TCCATCCCTT ATGAAGCTTC AGGAAGTACA
    GTGATATCAG AGATTCCCAA ACTCCCCGCC CCCTTGCGCT CTCCATCGGC TAATAGGATG
    CTCCGCAGAC AGGAAGCAAT CAGAACCGAC TCTTTGGAAT TTTTTCCCGA TAGCAAAGAA
    AATGCTGGCA TAACTGAGCC CCCACCTGTA TATATTCCAA TGTGCCAGGT GCCCACCGAG
    ACACCCTCGG CTTTTATAGA ACCAGAAAGA ACTTTATGCC AGTTGAAGCG ACGCAGTGTT
    TACACCCAGC AAGTCCAAGA TACCTGCAAG ATGTTTAGGT ACCCTGATGC TTCTCAAACC
    AACATGGACA CGTTACGAGA AACCTCAAAG ATTCCAGAGA AAGAAGATTT GTTGAGCGCA
    GCATGTTGTA GGCGGCATCT CAACTCCAAA ACGTGTAAAG CATTAAGCGT CTATTCTGCA
    GCATACTCCT TAAGCCAGAA ACTGAAACTA GGTAACAGCG GCAACGTTAT TTTGAAGGAC
    ATGAAGAGGT TCTACAGCGT AGATGCCCAC GGCGTCCTTA AAAAGACATC CTCTTGGTTG
    GATGACCCCA GAAGACATTC CATAGAAATT TGTTCTTTAG AGAACACTCT TCATCACTAC
    TCTACTTCAC GTTCTTGTGG ATTCATTAGC CACGTATTCC AGAAGAAGGA GGACTTGCAA
    ATAGTGCAGA GGACGGGCGG ACAGGATCCC GTCTGTCCAG ATTGTGTTCA CTCAGGAGAA
    GCATCAGAAC TACCACTCTC TTACGTCTCG AGTGGGCAAT CACAATCGAC AGACTCGACC
    GAGCTCGGTA AAATAGACTC TTTCGTTCCG GAAATGCAGA CATCCCCTAA ACCACTGGGA
    GATTTAGTTT CTGTCGAAAG TTTTTCTATG GATGCAGTTG AATTAAATCA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa17232
    Clone ID Related Accession (Same CDS sequence) XM_018097891.1 , XM_018097892.1
    Accession Version XM_018097891.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGATAAAT CTCATGAGCA AAGACCTGCC AAAGACCCTG GGGATCCGGG GTCGGAACTG 
    GAAGGGCTGC CCTACCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ACGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTTCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGAGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATTGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCTTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AATCTGATGC TGGATGATGT AATATTGGAG CACTCAGCCA GTCCAGTTAA AGAGGCCCAG
    TGCAAACCCT ACTACTCGGA GTATTCTCAC CCTCGGCTGC TCATCCATCA AATGTGCACA
    AGCCATTATC TGGATTTGTT TATTACTGGG GTCATCGGAT TGAATGTTAT CACTATGGCG
    ATGGAGCACT ACCAGCAACC CAAGGTATTG GATGAAGCTT TAAAAATCTG TAATTACATA
    TTTACCCTCA TTTTTGTGCT GGAATCTGGG TGCAAAATGG TAGCTTTTGG CTTCCGGCGA
    TTCTTCAAAG ACAGGTGGAA CCAGTTGGAT TTGGCCATTG TTCTGCTCTC CATTATGGGG
    ATCACCCTGG AGGAGATTGA AGTCAATGCA TCGCTGCCCA TTAACCCAAC AATCATCAGA
    ATAATGAGGG TCCTGCGGAT CGCTCGCGTG CTGAAGTTAT TAAAGATGGC AGTCGGTATG
    CGAGCCCTGC TGGATACAGT ATTGCAAGCG CTTCCTCAAG TGGGGAATCT CGGCCTGCTC
    TTTATGCTGC TGTTTTTCAT ATTTGCCGCT TTGGGAGTTG AACTTTTTGG CGACTTAGAA
    TGCACTGATT CCCACCCGTG TGAAGGTCTC GGCAGGCACG CCACGTTCAG GAACTTCGGC
    ATGGCCTTTC TGACTTTGTT CAGAGTATCC ACAGGAGATA ACTGGAACGG TATAATGAAG
    GACACACTCC GTGATTGCAA CGAGGATTCC ACCTGTTACA ACACAGTCAT CTCCCCCATC
    TATTTTGTCT CTTTTGTGCT GACTGCCCAG TTTGTCCTGG TCAATGTGGT AATAGCGGTG
    CTCATGAAAC ACCTTGAGGA AAGCAACAAG GAAGCTAAGG AGGAGGCGGA GCTGGAGGCA
    GAGATGGAAC TAGAGTTTCA GATCCAGAAC ATGCAGGTTC CGGATCCTGA CCCATTTATG
    TGGGCTGGGA GAGATCTCGG CTATCCCCAG GAAGGTTCTT ACGGATATAT GAACTCCGTG
    CAGGACAAGG CCGACTCACA GCTTTCATTA GTCAATCCCA TGGAAAGACA CATGTTTGAT
    ACAATATCAC TACTGATCCA GGGATCTTTG GAAGGAGAGA TGAAGCTGAT GGACAATTTG
    TCTGGCTCTG TGTGCCACCA TTACGCCCTC CCTCCCTCTG ACAAGGGCTC CTCAGAAAAA
    CAGATCCCAT TGGCAGAGAT GGAAGGCCTA TCCATGACAT CAGACATTCT GTCCGAGAAA
    TCATGGTCCT TAGCTCTGAC GGACGACTCC CTGCCAGATG ACCCTAATCT CAACCCATTT
    GGTGTACTGG AAACCAAACT TGGCCTCCCC GAGGAGAAAC ATTCGGCAAC CGTTGAGGAG
    GCTACGAATC TTCTCAGCGT CCGGAAACCG TCAGTATGCC GCACACATTC CCTGCCCAAT
    GACAGCTACA TGTTGCAAAC GGGAAGCACG TGCGCTGAGT GCCAGGAGCA AAGAAACCAC
    TGTTACCAGA AATCAGGTTC CAGCTCAACG GTTCAGTCTC AGCCAAATGA CCTCAACTCT
    CTTCTCCAAG TGCCACAAAT CTCCATCCCT TATGAAGCTT CAGGAAGTAC AGTGATATCA
    GAGATTCCCA AACTCCCCGC CCCCTTGCGC TCTCCATCGG CTAATAGGAT GCTCCGCAGA
    CAGGAAGCAA TCAGAACCGA CTCTTTGGAA TTTTTTCCCG ATAGCAAAGA AAATGCTGGC
    ATAACTGAGC CCCCACCTGT ATATATTCCA ATGTGCCAGG TGCCCACCGA GACACCCTCG
    GCTTTTATAG AACCAGAAAG AACTTTATGC CAGTTGAAGC GACGCAGTGT TTACACCCAG
    CAAGTCCAAG ATACCTGCAA GATGTTTAGG TACCCTGATG CTTCTCAAAC CAACATGGAC
    ACGTTACGAG AAACCTCAAA GATTCCAGAG AAAGAAGATT TGTTGAGCGC AGCATGTTGT
    AGGCGGCATC TCAACTCCAA AACGTGTAAA GCATTAAGCG TCTATTCTGC AGCATACTCC
    TTAAGCCAGA AACTGAAACT AGGTAACAGC GGCAACGTTA TTTTGAAGGA CATGAAGAGG
    TTCTACAGCG TAGATGCCCA CGGCGTCCTT AAAAAGACAT CCTCTTGGTT GGATGACCCC
    AGAAGACATT CCATAGAAAT TTGTTCTTTA GAGAACACTC TTCATCACTA CTCTACTTCA
    CGTTCTTGTG GATTCATTAG CCACGTATTC CAGAAGAAGG AGGACTTGCA AATAGTGCAG
    AGGACGGGCG GACAGGATCC CGTCTGTCCA GATTGTGTTC ACTCAGGAGA AGCATCAGAA
    CTACCACTCT CTTACGTCTC GAGTGGGCAA TCACAATCGA CAGACTCGAC CGAGCTCGCT
    AAAATAGACT CTTTCGTTCC GGAAATGCAG ACATCCCCTA AACCACTGGG AGATTTAGTT
    TCTGTCGAAA GTTTTTCTAT GGATGCAGTT GAATTAAATC AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017953380.1
    CDS1624..8688
    Translation

    Target ORF information:

    RefSeq Version XM_018097891.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018097891.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGATAAAT CTCATGAGCA AAGACCTGCC AAAGACCCTG GGGATCCGGG GTCGGAACTG 
    GAAGGGCTGC CCTACCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ACGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTTCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGAGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATTGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCTTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AATCTGATGC TGGATGATGT AATATTGGAG CACTCAGCCA GTCCAGTTAA AGAGGCCCAG
    TGCAAACCCT ACTACTCGGA GTATTCTCAC CCTCGGCTGC TCATCCATCA AATGTGCACA
    AGCCATTATC TGGATTTGTT TATTACTGGG GTCATCGGAT TGAATGTTAT CACTATGGCG
    ATGGAGCACT ACCAGCAACC CAAGGTATTG GATGAAGCTT TAAAAATCTG TAATTACATA
    TTTACCCTCA TTTTTGTGCT GGAATCTGGG TGCAAAATGG TAGCTTTTGG CTTCCGGCGA
    TTCTTCAAAG ACAGGTGGAA CCAGTTGGAT TTGGCCATTG TTCTGCTCTC CATTATGGGG
    ATCACCCTGG AGGAGATTGA AGTCAATGCA TCGCTGCCCA TTAACCCAAC AATCATCAGA
    ATAATGAGGG TCCTGCGGAT CGCTCGCGTG CTGAAGTTAT TAAAGATGGC AGTCGGTATG
    CGAGCCCTGC TGGATACAGT ATTGCAAGCG CTTCCTCAAG TGGGGAATCT CGGCCTGCTC
    TTTATGCTGC TGTTTTTCAT ATTTGCCGCT TTGGGAGTTG AACTTTTTGG CGACTTAGAA
    TGCACTGATT CCCACCCGTG TGAAGGTCTC GGCAGGCACG CCACGTTCAG GAACTTCGGC
    ATGGCCTTTC TGACTTTGTT CAGAGTATCC ACAGGAGATA ACTGGAACGG TATAATGAAG
    GACACACTCC GTGATTGCAA CGAGGATTCC ACCTGTTACA ACACAGTCAT CTCCCCCATC
    TATTTTGTCT CTTTTGTGCT GACTGCCCAG TTTGTCCTGG TCAATGTGGT AATAGCGGTG
    CTCATGAAAC ACCTTGAGGA AAGCAACAAG GAAGCTAAGG AGGAGGCGGA GCTGGAGGCA
    GAGATGGAAC TAGAGTTTCA GATCCAGAAC ATGCAGGTTC CGGATCCTGA CCCATTTATG
    TGGGCTGGGA GAGATCTCGG CTATCCCCAG GAAGGTTCTT ACGGATATAT GAACTCCGTG
    CAGGACAAGG CCGACTCACA GCTTTCATTA GTCAATCCCA TGGAAAGACA CATGTTTGAT
    ACAATATCAC TACTGATCCA GGGATCTTTG GAAGGAGAGA TGAAGCTGAT GGACAATTTG
    TCTGGCTCTG TGTGCCACCA TTACGCCCTC CCTCCCTCTG ACAAGGGCTC CTCAGAAAAA
    CAGATCCCAT TGGCAGAGAT GGAAGGCCTA TCCATGACAT CAGACATTCT GTCCGAGAAA
    TCATGGTCCT TAGCTCTGAC GGACGACTCC CTGCCAGATG ACCCTAATCT CAACCCATTT
    GGTGTACTGG AAACCAAACT TGGCCTCCCC GAGGAGAAAC ATTCGGCAAC CGTTGAGGAG
    GCTACGAATC TTCTCAGCGT CCGGAAACCG TCAGTATGCC GCACACATTC CCTGCCCAAT
    GACAGCTACA TGTTGCAAAC GGGAAGCACG TGCGCTGAGT GCCAGGAGCA AAGAAACCAC
    TGTTACCAGA AATCAGGTTC CAGCTCAACG GTTCAGTCTC AGCCAAATGA CCTCAACTCT
    CTTCTCCAAG TGCCACAAAT CTCCATCCCT TATGAAGCTT CAGGAAGTAC AGTGATATCA
    GAGATTCCCA AACTCCCCGC CCCCTTGCGC TCTCCATCGG CTAATAGGAT GCTCCGCAGA
    CAGGAAGCAA TCAGAACCGA CTCTTTGGAA TTTTTTCCCG ATAGCAAAGA AAATGCTGGC
    ATAACTGAGC CCCCACCTGT ATATATTCCA ATGTGCCAGG TGCCCACCGA GACACCCTCG
    GCTTTTATAG AACCAGAAAG AACTTTATGC CAGTTGAAGC GACGCAGTGT TTACACCCAG
    CAAGTCCAAG ATACCTGCAA GATGTTTAGG TACCCTGATG CTTCTCAAAC CAACATGGAC
    ACGTTACGAG AAACCTCAAA GATTCCAGAG AAAGAAGATT TGTTGAGCGC AGCATGTTGT
    AGGCGGCATC TCAACTCCAA AACGTGTAAA GCATTAAGCG TCTATTCTGC AGCATACTCC
    TTAAGCCAGA AACTGAAACT AGGTAACAGC GGCAACGTTA TTTTGAAGGA CATGAAGAGG
    TTCTACAGCG TAGATGCCCA CGGCGTCCTT AAAAAGACAT CCTCTTGGTT GGATGACCCC
    AGAAGACATT CCATAGAAAT TTGTTCTTTA GAGAACACTC TTCATCACTA CTCTACTTCA
    CGTTCTTGTG GATTCATTAG CCACGTATTC CAGAAGAAGG AGGACTTGCA AATAGTGCAG
    AGGACGGGCG GACAGGATCC CGTCTGTCCA GATTGTGTTC ACTCAGGAGA AGCATCAGAA
    CTACCACTCT CTTACGTCTC GAGTGGGCAA TCACAATCGA CAGACTCGAC CGAGCTCGCT
    AAAATAGACT CTTTCGTTCC GGAAATGCAG ACATCCCCTA AACCACTGGG AGATTTAGTT
    TCTGTCGAAA GTTTTTCTAT GGATGCAGTT GAATTAAATC AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa17232
    Clone ID Related Accession (Same CDS sequence) XM_018097891.1 , XM_018097892.1
    Accession Version XM_018097892.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGATAAAT CTCATGAGCA AAGACCTGCC AAAGACCCTG GGGATCCGGG GTCGGAACTG 
    GAAGGGCTGC CCTACCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ACGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTTCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGAGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATTGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCTTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AATCTGATGC TGGATGATGT AATATTGGAG CACTCAGCCA GTCCAGTTAA AGAGGCCCAG
    TGCAAACCCT ACTACTCGGA GTATTCTCAC CCTCGGCTGC TCATCCATCA AATGTGCACA
    AGCCATTATC TGGATTTGTT TATTACTGGG GTCATCGGAT TGAATGTTAT CACTATGGCG
    ATGGAGCACT ACCAGCAACC CAAGGTATTG GATGAAGCTT TAAAAATCTG TAATTACATA
    TTTACCCTCA TTTTTGTGCT GGAATCTGGG TGCAAAATGG TAGCTTTTGG CTTCCGGCGA
    TTCTTCAAAG ACAGGTGGAA CCAGTTGGAT TTGGCCATTG TTCTGCTCTC CATTATGGGG
    ATCACCCTGG AGGAGATTGA AGTCAATGCA TCGCTGCCCA TTAACCCAAC AATCATCAGA
    ATAATGAGGG TCCTGCGGAT CGCTCGCGTG CTGAAGTTAT TAAAGATGGC AGTCGGTATG
    CGAGCCCTGC TGGATACAGT ATTGCAAGCG CTTCCTCAAG TGGGGAATCT CGGCCTGCTC
    TTTATGCTGC TGTTTTTCAT ATTTGCCGCT TTGGGAGTTG AACTTTTTGG CGACTTAGAA
    TGCACTGATT CCCACCCGTG TGAAGGTCTC GGCAGGCACG CCACGTTCAG GAACTTCGGC
    ATGGCCTTTC TGACTTTGTT CAGAGTATCC ACAGGAGATA ACTGGAACGG TATAATGAAG
    GACACACTCC GTGATTGCAA CGAGGATTCC ACCTGTTACA ACACAGTCAT CTCCCCCATC
    TATTTTGTCT CTTTTGTGCT GACTGCCCAG TTTGTCCTGG TCAATGTGGT AATAGCGGTG
    CTCATGAAAC ACCTTGAGGA AAGCAACAAG GAAGCTAAGG AGGAGGCGGA GCTGGAGGCA
    GAGATGGAAC TAGAGTTTCA GATCCAGAAC ATGCAGGTTC CGGATCCTGA CCCATTTATG
    TGGGCTGGGA GAGATCTCGG CTATCCCCAG GAAGGTTCTT ACGGATATAT GAACTCCGTG
    CAGGACAAGG CCGACTCACA GCTTTCATTA GTCAATCCCA TGGAAAGACA CATGTTTGAT
    ACAATATCAC TACTGATCCA GGGATCTTTG GAAGGAGAGA TGAAGCTGAT GGACAATTTG
    TCTGGCTCTG TGTGCCACCA TTACGCCCTC CCTCCCTCTG ACAAGGGCTC CTCAGAAAAA
    CAGATCCCAT TGGCAGAGAT GGAAGGCCTA TCCATGACAT CAGACATTCT GTCCGAGAAA
    TCATGGTCCT TAGCTCTGAC GGACGACTCC CTGCCAGATG ACCCTAATCT CAACCCATTT
    GGTGTACTGG AAACCAAACT TGGCCTCCCC GAGGAGAAAC ATTCGGCAAC CGTTGAGGAG
    GCTACGAATC TTCTCAGCGT CCGGAAACCG TCAGTATGCC GCACACATTC CCTGCCCAAT
    GACAGCTACA TGTTGCAAAC GGGAAGCACG TGCGCTGAGT GCCAGGAGCA AAGAAACCAC
    TGTTACCAGA AATCAGGTTC CAGCTCAACG GTTCAGTCTC AGCCAAATGA CCTCAACTCT
    CTTCTCCAAG TGCCACAAAT CTCCATCCCT TATGAAGCTT CAGGAAGTAC AGTGATATCA
    GAGATTCCCA AACTCCCCGC CCCCTTGCGC TCTCCATCGG CTAATAGGAT GCTCCGCAGA
    CAGGAAGCAA TCAGAACCGA CTCTTTGGAA TTTTTTCCCG ATAGCAAAGA AAATGCTGGC
    ATAACTGAGC CCCCACCTGT ATATATTCCA ATGTGCCAGG TGCCCACCGA GACACCCTCG
    GCTTTTATAG AACCAGAAAG AACTTTATGC CAGTTGAAGC GACGCAGTGT TTACACCCAG
    CAAGTCCAAG ATACCTGCAA GATGTTTAGG TACCCTGATG CTTCTCAAAC CAACATGGAC
    ACGTTACGAG AAACCTCAAA GATTCCAGAG AAAGAAGATT TGTTGAGCGC AGCATGTTGT
    AGGCGGCATC TCAACTCCAA AACGTGTAAA GCATTAAGCG TCTATTCTGC AGCATACTCC
    TTAAGCCAGA AACTGAAACT AGGTAACAGC GGCAACGTTA TTTTGAAGGA CATGAAGAGG
    TTCTACAGCG TAGATGCCCA CGGCGTCCTT AAAAAGACAT CCTCTTGGTT GGATGACCCC
    AGAAGACATT CCATAGAAAT TTGTTCTTTA GAGAACACTC TTCATCACTA CTCTACTTCA
    CGTTCTTGTG GATTCATTAG CCACGTATTC CAGAAGAAGG AGGACTTGCA AATAGTGCAG
    AGGACGGGCG GACAGGATCC CGTCTGTCCA GATTGTGTTC ACTCAGGAGA AGCATCAGAA
    CTACCACTCT CTTACGTCTC GAGTGGGCAA TCACAATCGA CAGACTCGAC CGAGCTCGCT
    AAAATAGACT CTTTCGTTCC GGAAATGCAG ACATCCCCTA AACCACTGGG AGATTTAGTT
    TCTGTCGAAA GTTTTTCTAT GGATGCAGTT GAATTAAATC AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017953381.1
    CDS1936..9000
    Translation

    Target ORF information:

    RefSeq Version XM_018097892.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018097892.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGATAAAT CTCATGAGCA AAGACCTGCC AAAGACCCTG GGGATCCGGG GTCGGAACTG 
    GAAGGGCTGC CCTACCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ACGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTTCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGAGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATTGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCTTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AATCTGATGC TGGATGATGT AATATTGGAG CACTCAGCCA GTCCAGTTAA AGAGGCCCAG
    TGCAAACCCT ACTACTCGGA GTATTCTCAC CCTCGGCTGC TCATCCATCA AATGTGCACA
    AGCCATTATC TGGATTTGTT TATTACTGGG GTCATCGGAT TGAATGTTAT CACTATGGCG
    ATGGAGCACT ACCAGCAACC CAAGGTATTG GATGAAGCTT TAAAAATCTG TAATTACATA
    TTTACCCTCA TTTTTGTGCT GGAATCTGGG TGCAAAATGG TAGCTTTTGG CTTCCGGCGA
    TTCTTCAAAG ACAGGTGGAA CCAGTTGGAT TTGGCCATTG TTCTGCTCTC CATTATGGGG
    ATCACCCTGG AGGAGATTGA AGTCAATGCA TCGCTGCCCA TTAACCCAAC AATCATCAGA
    ATAATGAGGG TCCTGCGGAT CGCTCGCGTG CTGAAGTTAT TAAAGATGGC AGTCGGTATG
    CGAGCCCTGC TGGATACAGT ATTGCAAGCG CTTCCTCAAG TGGGGAATCT CGGCCTGCTC
    TTTATGCTGC TGTTTTTCAT ATTTGCCGCT TTGGGAGTTG AACTTTTTGG CGACTTAGAA
    TGCACTGATT CCCACCCGTG TGAAGGTCTC GGCAGGCACG CCACGTTCAG GAACTTCGGC
    ATGGCCTTTC TGACTTTGTT CAGAGTATCC ACAGGAGATA ACTGGAACGG TATAATGAAG
    GACACACTCC GTGATTGCAA CGAGGATTCC ACCTGTTACA ACACAGTCAT CTCCCCCATC
    TATTTTGTCT CTTTTGTGCT GACTGCCCAG TTTGTCCTGG TCAATGTGGT AATAGCGGTG
    CTCATGAAAC ACCTTGAGGA AAGCAACAAG GAAGCTAAGG AGGAGGCGGA GCTGGAGGCA
    GAGATGGAAC TAGAGTTTCA GATCCAGAAC ATGCAGGTTC CGGATCCTGA CCCATTTATG
    TGGGCTGGGA GAGATCTCGG CTATCCCCAG GAAGGTTCTT ACGGATATAT GAACTCCGTG
    CAGGACAAGG CCGACTCACA GCTTTCATTA GTCAATCCCA TGGAAAGACA CATGTTTGAT
    ACAATATCAC TACTGATCCA GGGATCTTTG GAAGGAGAGA TGAAGCTGAT GGACAATTTG
    TCTGGCTCTG TGTGCCACCA TTACGCCCTC CCTCCCTCTG ACAAGGGCTC CTCAGAAAAA
    CAGATCCCAT TGGCAGAGAT GGAAGGCCTA TCCATGACAT CAGACATTCT GTCCGAGAAA
    TCATGGTCCT TAGCTCTGAC GGACGACTCC CTGCCAGATG ACCCTAATCT CAACCCATTT
    GGTGTACTGG AAACCAAACT TGGCCTCCCC GAGGAGAAAC ATTCGGCAAC CGTTGAGGAG
    GCTACGAATC TTCTCAGCGT CCGGAAACCG TCAGTATGCC GCACACATTC CCTGCCCAAT
    GACAGCTACA TGTTGCAAAC GGGAAGCACG TGCGCTGAGT GCCAGGAGCA AAGAAACCAC
    TGTTACCAGA AATCAGGTTC CAGCTCAACG GTTCAGTCTC AGCCAAATGA CCTCAACTCT
    CTTCTCCAAG TGCCACAAAT CTCCATCCCT TATGAAGCTT CAGGAAGTAC AGTGATATCA
    GAGATTCCCA AACTCCCCGC CCCCTTGCGC TCTCCATCGG CTAATAGGAT GCTCCGCAGA
    CAGGAAGCAA TCAGAACCGA CTCTTTGGAA TTTTTTCCCG ATAGCAAAGA AAATGCTGGC
    ATAACTGAGC CCCCACCTGT ATATATTCCA ATGTGCCAGG TGCCCACCGA GACACCCTCG
    GCTTTTATAG AACCAGAAAG AACTTTATGC CAGTTGAAGC GACGCAGTGT TTACACCCAG
    CAAGTCCAAG ATACCTGCAA GATGTTTAGG TACCCTGATG CTTCTCAAAC CAACATGGAC
    ACGTTACGAG AAACCTCAAA GATTCCAGAG AAAGAAGATT TGTTGAGCGC AGCATGTTGT
    AGGCGGCATC TCAACTCCAA AACGTGTAAA GCATTAAGCG TCTATTCTGC AGCATACTCC
    TTAAGCCAGA AACTGAAACT AGGTAACAGC GGCAACGTTA TTTTGAAGGA CATGAAGAGG
    TTCTACAGCG TAGATGCCCA CGGCGTCCTT AAAAAGACAT CCTCTTGGTT GGATGACCCC
    AGAAGACATT CCATAGAAAT TTGTTCTTTA GAGAACACTC TTCATCACTA CTCTACTTCA
    CGTTCTTGTG GATTCATTAG CCACGTATTC CAGAAGAAGG AGGACTTGCA AATAGTGCAG
    AGGACGGGCG GACAGGATCC CGTCTGTCCA GATTGTGTTC ACTCAGGAGA AGCATCAGAA
    CTACCACTCT CTTACGTCTC GAGTGGGCAA TCACAATCGA CAGACTCGAC CGAGCTCGCT
    AAAATAGACT CTTTCGTTCC GGAAATGCAG ACATCCCCTA AACCACTGGG AGATTTAGTT
    TCTGTCGAAA GTTTTTCTAT GGATGCAGTT GAATTAAATC AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa17234
    Clone ID Related Accession (Same CDS sequence) XM_004918488.2
    Accession Version XM_004918488.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7035bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004918488.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGATAAAT CTCATGAGCA AAGACCTGCC AAAGACCCTG GGGATCCGGG GTCGGAACTG 
    GAAGGGCTGC CCTACCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ACGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTTCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGAGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATTGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCTTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AGTAAGGAGA GAGAGCTGGC CGAGGCCCAG TGCAAACCCT ACTACTCGGA GTATTCTCAC
    CCTCGGCTGC TCATCCATCA AATGTGCACA AGCCATTATC TGGATTTGTT TATTACTGGG
    GTCATCGGAT TGAATGTTAT CACTATGGCG ATGGAGCACT ACCAGCAACC CAAGGTATTG
    GATGAAGCTT TAAAAATCTG TAATTACATA TTTACCCTCA TTTTTGTGCT GGAATCTGGG
    TGCAAAATGG TAGCTTTTGG CTTCCGGCGA TTCTTCAAAG ACAGGTGGAA CCAGTTGGAT
    TTGGCCATTG TTCTGCTCTC CATTATGGGG ATCACCCTGG AGGAGATTGA AGTCAATGCA
    TCGCTGCCCA TTAACCCAAC AATCATCAGA ATAATGAGGG TCCTGCGGAT CGCTCGCGTG
    CTGAAGTTAT TAAAGATGGC AGTCGGTATG CGAGCCCTGC TGGATACAGT ATTGCAAGCG
    CTTCCTCAAG TGGGGAATCT CGGCCTGCTC TTTATGCTGC TGTTTTTCAT ATTTGCCGCT
    TTGGGAGTTG AACTTTTTGG CGACTTAGAA TGCACTGATT CCCACCCGTG TGAAGGTCTC
    GGCAGGCACG CCACGTTCAG GAACTTCGGC ATGGCCTTTC TGACTTTGTT CAGAGTATCC
    ACAGGAGATA ACTGGAACGG TATAATGAAG GACACACTCC GTGATTGCAA CGAGGATTCC
    ACCTGTTACA ACACAGTCAT CTCCCCCATC TATTTTGTCT CTTTTGTGCT GACTGCCCAG
    TTTGTCCTGG TCAATGTGGT AATAGCGGTG CTCATGAAAC ACCTTGAGGA AAGCAACAAG
    GAAGCTAAGG AGGAGGCGGA GCTGGAGGCA GAGATGGAAC TAGAGTTTCA GATCCAGAAC
    ATGCAGGTTC CGGATCCTGA CCCATTTATG TGGGCTGGGA GAGATCTCGG CTATCCCCAG
    GAAGGTTCTT ACGGATATAT GAACTCCGTG CAGGACAAGG CCGACTCACA GCTTTCATTA
    GTCAATCCCA TGGAAAGACA CATGTTTGAT ACAATATCAC TACTGATCCA GGGATCTTTG
    GAAGGAGAGA TGAAGCTGAT GGACAATTTG TCTGGCTCTG TGTGCCACCA TTACGCCCTC
    CCTCCCTCTG ACAAGGGCTC CTCAGAAAAA CAGATCCCAT TGGCAGAGAT GGAAGGCCTA
    TCCATGACAT CAGACATTCT GTCCGAGAAA TCATGGTCCT TAGCTCTGAC GGACGACTCC
    CTGCCAGATG ACCCTAATCT CAACCCATTT GGTGTACTGG AAACCAAACT TGGCCTCCCC
    GAGGAGAAAC ATTCGGCAAC CGTTGAGGAG GCTACGAATC TTCTCAGCGT CCGGAAACCG
    TCAGTATGCC GCACACATTC CCTGCCCAAT GACAGCTACA TGTTGCAAAC GGGAAGCACG
    TGCGCTGAGT GCCAGGAGCA AAGAAACCAC TGTTACCAGA AATCAGGTTC CAGCTCAACG
    GTTCAGTCTC AGCCAAATGA CCTCAACTCT CTTCTCCAAG TGCCACAAAT CTCCATCCCT
    TATGAAGCTT CAGGAAGTAC AGTGATATCA GAGATTCCCA AACTCCCCGC CCCCTTGCGC
    TCTCCATCGG CTAATAGGAT GCTCCGCAGA CAGGAAGCAA TCAGAACCGA CTCTTTGGAA
    TTTTTTCCCG ATAGCAAAGA AAATGCTGGC ATAACTGAGC CCCCACCTGT ATATATTCCA
    ATGTGCCAGG TGCCCACCGA GACACCCTCG GCTTTTATAG AACCAGAAAG AACTTTATGC
    CAGTTGAAGC GACGCAGTGT TTACACCCAG CAAGTCCAAG ATACCTGCAA GATGTTTAGG
    TACCCTGATG CTTCTCAAAC CAACATGGAC ACGTTACGAG AAACCTCAAA GATTCCAGAG
    AAAGAAGATT TGTTGAGCGC AGCATGTTGT AGGCGGCATC TCAACTCCAA AACGTGTAAA
    GCATTAAGCG TCTATTCTGC AGCATACTCC TTAAGCCAGA AACTGAAACT AGGTAACAGC
    GGCAACGTTA TTTTGAAGGA CATGAAGAGG TTCTACAGCG TAGATGCCCA CGGCGTCCTT
    AAAAAGACAT CCTCTTGGTT GGATGACCCC AGAAGACATT CCATAGAAAT TTGTTCTTTA
    GAGAACACTC TTCATCACTA CTCTACTTCA CGTTCTTGTG GATTCATTAG CCACGTATTC
    CAGAAGAAGG AGGACTTGCA AATAGTGCAG AGGACGGGCG GACAGGATCC CGTCTGTCCA
    GATTGTGTTC ACTCAGGAGA AGCATCAGAA CTACCACTCT CTTACGTCTC GAGTGGGCAA
    TCACAATCGA CAGACTCGAC CGAGCTCGCT AAAATAGACT CTTTCGTTCC GGAAATGCAG
    ACATCCCCTA AACCACTGGG AGATTTAGTT TCTGTCGAAA GTTTTTCTAT GGATGCAGTT
    GAATTAAATC AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004918545.1
    CDS1664..8698
    Translation

    Target ORF information:

    RefSeq Version XM_004918488.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004918488.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGATAAAT CTCATGAGCA AAGACCTGCC AAAGACCCTG GGGATCCGGG GTCGGAACTG 
    GAAGGGCTGC CCTACCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ACGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTTCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGAGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATTGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCTTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AGTAAGGAGA GAGAGCTGGC CGAGGCCCAG TGCAAACCCT ACTACTCGGA GTATTCTCAC
    CCTCGGCTGC TCATCCATCA AATGTGCACA AGCCATTATC TGGATTTGTT TATTACTGGG
    GTCATCGGAT TGAATGTTAT CACTATGGCG ATGGAGCACT ACCAGCAACC CAAGGTATTG
    GATGAAGCTT TAAAAATCTG TAATTACATA TTTACCCTCA TTTTTGTGCT GGAATCTGGG
    TGCAAAATGG TAGCTTTTGG CTTCCGGCGA TTCTTCAAAG ACAGGTGGAA CCAGTTGGAT
    TTGGCCATTG TTCTGCTCTC CATTATGGGG ATCACCCTGG AGGAGATTGA AGTCAATGCA
    TCGCTGCCCA TTAACCCAAC AATCATCAGA ATAATGAGGG TCCTGCGGAT CGCTCGCGTG
    CTGAAGTTAT TAAAGATGGC AGTCGGTATG CGAGCCCTGC TGGATACAGT ATTGCAAGCG
    CTTCCTCAAG TGGGGAATCT CGGCCTGCTC TTTATGCTGC TGTTTTTCAT ATTTGCCGCT
    TTGGGAGTTG AACTTTTTGG CGACTTAGAA TGCACTGATT CCCACCCGTG TGAAGGTCTC
    GGCAGGCACG CCACGTTCAG GAACTTCGGC ATGGCCTTTC TGACTTTGTT CAGAGTATCC
    ACAGGAGATA ACTGGAACGG TATAATGAAG GACACACTCC GTGATTGCAA CGAGGATTCC
    ACCTGTTACA ACACAGTCAT CTCCCCCATC TATTTTGTCT CTTTTGTGCT GACTGCCCAG
    TTTGTCCTGG TCAATGTGGT AATAGCGGTG CTCATGAAAC ACCTTGAGGA AAGCAACAAG
    GAAGCTAAGG AGGAGGCGGA GCTGGAGGCA GAGATGGAAC TAGAGTTTCA GATCCAGAAC
    ATGCAGGTTC CGGATCCTGA CCCATTTATG TGGGCTGGGA GAGATCTCGG CTATCCCCAG
    GAAGGTTCTT ACGGATATAT GAACTCCGTG CAGGACAAGG CCGACTCACA GCTTTCATTA
    GTCAATCCCA TGGAAAGACA CATGTTTGAT ACAATATCAC TACTGATCCA GGGATCTTTG
    GAAGGAGAGA TGAAGCTGAT GGACAATTTG TCTGGCTCTG TGTGCCACCA TTACGCCCTC
    CCTCCCTCTG ACAAGGGCTC CTCAGAAAAA CAGATCCCAT TGGCAGAGAT GGAAGGCCTA
    TCCATGACAT CAGACATTCT GTCCGAGAAA TCATGGTCCT TAGCTCTGAC GGACGACTCC
    CTGCCAGATG ACCCTAATCT CAACCCATTT GGTGTACTGG AAACCAAACT TGGCCTCCCC
    GAGGAGAAAC ATTCGGCAAC CGTTGAGGAG GCTACGAATC TTCTCAGCGT CCGGAAACCG
    TCAGTATGCC GCACACATTC CCTGCCCAAT GACAGCTACA TGTTGCAAAC GGGAAGCACG
    TGCGCTGAGT GCCAGGAGCA AAGAAACCAC TGTTACCAGA AATCAGGTTC CAGCTCAACG
    GTTCAGTCTC AGCCAAATGA CCTCAACTCT CTTCTCCAAG TGCCACAAAT CTCCATCCCT
    TATGAAGCTT CAGGAAGTAC AGTGATATCA GAGATTCCCA AACTCCCCGC CCCCTTGCGC
    TCTCCATCGG CTAATAGGAT GCTCCGCAGA CAGGAAGCAA TCAGAACCGA CTCTTTGGAA
    TTTTTTCCCG ATAGCAAAGA AAATGCTGGC ATAACTGAGC CCCCACCTGT ATATATTCCA
    ATGTGCCAGG TGCCCACCGA GACACCCTCG GCTTTTATAG AACCAGAAAG AACTTTATGC
    CAGTTGAAGC GACGCAGTGT TTACACCCAG CAAGTCCAAG ATACCTGCAA GATGTTTAGG
    TACCCTGATG CTTCTCAAAC CAACATGGAC ACGTTACGAG AAACCTCAAA GATTCCAGAG
    AAAGAAGATT TGTTGAGCGC AGCATGTTGT AGGCGGCATC TCAACTCCAA AACGTGTAAA
    GCATTAAGCG TCTATTCTGC AGCATACTCC TTAAGCCAGA AACTGAAACT AGGTAACAGC
    GGCAACGTTA TTTTGAAGGA CATGAAGAGG TTCTACAGCG TAGATGCCCA CGGCGTCCTT
    AAAAAGACAT CCTCTTGGTT GGATGACCCC AGAAGACATT CCATAGAAAT TTGTTCTTTA
    GAGAACACTC TTCATCACTA CTCTACTTCA CGTTCTTGTG GATTCATTAG CCACGTATTC
    CAGAAGAAGG AGGACTTGCA AATAGTGCAG AGGACGGGCG GACAGGATCC CGTCTGTCCA
    GATTGTGTTC ACTCAGGAGA AGCATCAGAA CTACCACTCT CTTACGTCTC GAGTGGGCAA
    TCACAATCGA CAGACTCGAC CGAGCTCGCT AAAATAGACT CTTTCGTTCC GGAAATGCAG
    ACATCCCCTA AACCACTGGG AGATTTAGTT TCTGTCGAAA GTTTTTCTAT GGATGCAGTT
    GAATTAAATC AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa17236
    Clone ID Related Accession (Same CDS sequence) XM_004918490.2
    Accession Version XM_004918490.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004918490.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGATAAAT CTCATGAGCA AAGACCTGCC AAAGACCCTG GGGATCCGGG GTCGGAACTG 
    GAAGGGCTGC CCTACCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ACGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTTCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGAGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATTGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCTTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AAGGCCCAGT GCAAACCCTA CTACTCGGAG TATTCTCACC CTCGGCTGCT CATCCATCAA
    ATGTGCACAA GCCATTATCT GGATTTGTTT ATTACTGGGG TCATCGGATT GAATGTTATC
    ACTATGGCGA TGGAGCACTA CCAGCAACCC AAGGTATTGG ATGAAGCTTT AAAAATCTGT
    AATTACATAT TTACCCTCAT TTTTGTGCTG GAATCTGGGT GCAAAATGGT AGCTTTTGGC
    TTCCGGCGAT TCTTCAAAGA CAGGTGGAAC CAGTTGGATT TGGCCATTGT TCTGCTCTCC
    ATTATGGGGA TCACCCTGGA GGAGATTGAA GTCAATGCAT CGCTGCCCAT TAACCCAACA
    ATCATCAGAA TAATGAGGGT CCTGCGGATC GCTCGCGTGC TGAAGTTATT AAAGATGGCA
    GTCGGTATGC GAGCCCTGCT GGATACAGTA TTGCAAGCGC TTCCTCAAGT GGGGAATCTC
    GGCCTGCTCT TTATGCTGCT GTTTTTCATA TTTGCCGCTT TGGGAGTTGA ACTTTTTGGC
    GACTTAGAAT GCACTGATTC CCACCCGTGT GAAGGTCTCG GCAGGCACGC CACGTTCAGG
    AACTTCGGCA TGGCCTTTCT GACTTTGTTC AGAGTATCCA CAGGAGATAA CTGGAACGGT
    ATAATGAAGG ACACACTCCG TGATTGCAAC GAGGATTCCA CCTGTTACAA CACAGTCATC
    TCCCCCATCT ATTTTGTCTC TTTTGTGCTG ACTGCCCAGT TTGTCCTGGT CAATGTGGTA
    ATAGCGGTGC TCATGAAACA CCTTGAGGAA AGCAACAAGG AAGCTAAGGA GGAGGCGGAG
    CTGGAGGCAG AGATGGAACT AGAGTTTCAG ATCCAGAACA TGCAGGTTCC GGATCCTGAC
    CCATTTATGT GGGCTGGGAG AGATCTCGGC TATCCCCAGG AAGGTTCTTA CGGATATATG
    AACTCCGTGC AGGACAAGGC CGACTCACAG CTTTCATTAG TCAATCCCAT GGAAAGACAC
    ATGTTTGATA CAATATCACT ACTGATCCAG GGATCTTTGG AAGGAGAGAT GAAGCTGATG
    GACAATTTGT CTGGCTCTGT GTGCCACCAT TACGCCCTCC CTCCCTCTGA CAAGGGCTCC
    TCAGAAAAAC AGATCCCATT GGCAGAGATG GAAGGCCTAT CCATGACATC AGACATTCTG
    TCCGAGAAAT CATGGTCCTT AGCTCTGACG GACGACTCCC TGCCAGATGA CCCTAATCTC
    AACCCATTTG GTGTACTGGA AACCAAACTT GGCCTCCCCG AGGAGAAACA TTCGGCAACC
    GTTGAGGAGG CTACGAATCT TCTCAGCGTC CGGAAACCGT CAGTATGCCG CACACATTCC
    CTGCCCAATG ACAGCTACAT GTTGCAAACG GGAAGCACGT GCGCTGAGTG CCAGGAGCAA
    AGAAACCACT GTTACCAGAA ATCAGGTTCC AGCTCAACGG TTCAGTCTCA GCCAAATGAC
    CTCAACTCTC TTCTCCAAGT GCCACAAATC TCCATCCCTT ATGAAGCTTC AGGAAGTACA
    GTGATATCAG AGATTCCCAA ACTCCCCGCC CCCTTGCGCT CTCCATCGGC TAATAGGATG
    CTCCGCAGAC AGGAAGCAAT CAGAACCGAC TCTTTGGAAT TTTTTCCCGA TAGCAAAGAA
    AATGCTGGCA TAACTGAGCC CCCACCTGTA TATATTCCAA TGTGCCAGGT GCCCACCGAG
    ACACCCTCGG CTTTTATAGA ACCAGAAAGA ACTTTATGCC AGTTGAAGCG ACGCAGTGTT
    TACACCCAGC AAGTCCAAGA TACCTGCAAG ATGTTTAGGT ACCCTGATGC TTCTCAAACC
    AACATGGACA CGTTACGAGA AACCTCAAAG ATTCCAGAGA AAGAAGATTT GTTGAGCGCA
    GCATGTTGTA GGCGGCATCT CAACTCCAAA ACGTGTAAAG CATTAAGCGT CTATTCTGCA
    GCATACTCCT TAAGCCAGAA ACTGAAACTA GGTAACAGCG GCAACGTTAT TTTGAAGGAC
    ATGAAGAGGT TCTACAGCGT AGATGCCCAC GGCGTCCTTA AAAAGACATC CTCTTGGTTG
    GATGACCCCA GAAGACATTC CATAGAAATT TGTTCTTTAG AGAACACTCT TCATCACTAC
    TCTACTTCAC GTTCTTGTGG ATTCATTAGC CACGTATTCC AGAAGAAGGA GGACTTGCAA
    ATAGTGCAGA GGACGGGCGG ACAGGATCCC GTCTGTCCAG ATTGTGTTCA CTCAGGAGAA
    GCATCAGAAC TACCACTCTC TTACGTCTCG AGTGGGCAAT CACAATCGAC AGACTCGACC
    GAGCTCGCTA AAATAGACTC TTTCGTTCCG GAAATGCAGA CATCCCCTAA ACCACTGGGA
    GATTTAGTTT CTGTCGAAAG TTTTTCTATG GATGCAGTTG AATTAAATCA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004918547.1
    CDS1664..8677
    Translation

    Target ORF information:

    RefSeq Version XM_004918490.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004918490.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGATAAAT CTCATGAGCA AAGACCTGCC AAAGACCCTG GGGATCCGGG GTCGGAACTG 
    GAAGGGCTGC CCTACCCCAC CTTGGCCCCA GTGGTGTTCT TCTTTCTGAC CCAAGAGAGC
    AGACCTAGGA GTTGGTGTCT AAAGATGGTG TGTAATCCCT GGTTTGAGCG CTTCAGTATG
    CTGGTCATCC TCCTCAACTG CGTGACTCTA GGGATGTTCC ACCCCTGTGA AGACATTGCT
    TGTGATTCTC AGCGCTGTAA GATCCTGCAG GGCTTCGATG ATTTCATTTT TGCCTTTTTC
    GCGGTGGAGA TGATTATAAA GATGTTGGCC CTGGGAATAT TCGGGAAGCA ATGCTATCTG
    GGAGACACCT GGAACTGCCT AGACCTCGTT ATAGTGGTGG CAGGGATGCT GGAATATTCT
    CTAGACCTCC AAAATGTCAC CTTCTCCGCA GTGCGCACTG TCAGAGTCCT CCGCCCGCTG
    AGAGCCATTA ACCGAGTGCC AAGTATGCGC ATTTTGGTGA CGTTGCTGCT GGACACCCTG
    CCGATGCTTG GGAACGTCCT TCTCCTGTGT TTCTTCGTTT TCTTCATCTT TGGGATTGTG
    GGAGTCCAAC TGTGGGCGGG GCTTCTACGA AACCGCTGCT TCCTTCCTGA AAACTTCAGT
    CTGCCGGTCT CCTTGGAATT GGAGAGATAC TACCAGACGG AAAATGAGGA TGAGAACCCA
    TTCATCTGCT CTCAGGCCAG AGAGAATGGG ATGCGCTACT GCCGCAACGT GCCCGCCATG
    AGAGAGGATG GCCTGGAATG TACACTGGAC CATTATTTCT ATAACAATAC CAGTAACACC
    TCCTGTGTCA ACTGGAACCA GTATTACACC AACTGTTCAG CTGGGGAGCA CAACCCATTC
    AAAGGTGCTA TCAATTTTGA TAATATTGGG TACGCCTGGA TAGCGATATT TCAGGTGATC
    ACTCTGGAGG GCTGGGTGGA TATCATGTAT TTTGTCATGG ATGCCCATTC CTTCTACAAC
    TTCATCTATT TTATTCTTCT CATTATAATC GGATCATTTT TCATGATCAA CCTGTGTCTC
    GTGGTCATCG CCACCCAGTT CTCGGAGACC AAGCAGCGGG AGAGCCAGCT GATGAAGGAG
    CAGCGGGTTC GCTTCCTCTC CAACGCCAGC ACGTTGGCCA GTTTCTCCGA GCCGGGCAGT
    TGCTACGACG AGCTGCTCAA ATACTTGGTG TATATAACCC GCAAGGGTAG CAAGAGGCTT
    GTGCAGTTCT ATCGAGCAGC CGCCTTCAAA GCGGGACTGT CGGCTAGGCC TGTGGTGAAG
    AACAGCGCGG AGAAGCCGAA TCGCAAGCAC AGGAGGAGGA GATCTTCTGT GCACCATCTA
    ATCCACCATC ACCACCATCA TCATCACCAT TATCACCTTG GAAACGGGAG CCTCCGTGCA
    CCACAGGCCA GCCCTGAGCT TAGCGAGGTA GAGACCAGTT CTCCTCACAA CACGTCGAGA
    CTGATGGTAC CGTTTGCTGG TTCCCCCATT GCTTCTGCCA CCAATACGCA GTCCATCCAT
    AGCATTTACC ATGCCGACTG CCACCTGGAA CCAGTACATT GTGTACAAAC CTTTGCCCAC
    CCTGGTTCTT CTTCAGAAAA CCCAGAGGTG CTCCCTAGAA GTATAACTGG AGGCAAAAAC
    TACCCTACTT TGCATCCAAG TGCGTCACCG GATATACAAG TGGATGCAGA ACCAGTGGAA
    CCATCAGAAA TCACCAGCGA TGCCAACATC CACCCCTTGC CAGTTTTTAA CTCAATGAAC
    AGGCTGCTAG AGACCCAGAG TACAGGTATT GGCAGAGGAG TCTGTAAGAT CTCAGGACAG
    ACGGCAAACC TTGAGTCTGG CTCTTGTGAT GGAGAGAAAT GTTCCCACTG CAAAGAGACC
    GAGCTCAGAG ATACCGAGGG CCCGGAGTCC GATAGTGACG GATGCTATGA ATTCACTCAA
    GACCCTCAGG GCAGTGAGCA GCAGCAGCAG ACAGAAGCCA AAAGAGCAAA GTCCAGGTCC
    CAAATCCCCA GCAAGCTGCA GCAGCTCTGG AAGATGGTCT GCGAAACCTT CAGGAAGATT
    GTCGACAGCA AATACTTCGG ACGGGGGATA ATGATCGCCA TCCTTATAAA CACCCTCAGC
    ATGGGGATTG AGTATCATGA GCAGCCAGAA GAACTGACCA ACGCGCTGGA GATCAGCAAT
    ATCGTCTTCA CCAGCCTTTT CGCTCTGGAA ATGCTCCTAA AGCTCTTGGT ATATGGTCCA
    TTCGGATACA TCAAGAACCC CTATAACATC TTTGATGGAA TTATTGTGGT TATCAGCGTC
    TGGGAGATCA TTGGCCAACA AGGAGGAGGT CTCTCCGTTC TGAGAACGTT CCGGCTAATG
    AGGGTTCTCA AACTGGTACG GTTCATGCCT GCTCTGCAGC GCCAGCTGGT GGTCCTAATG
    AAAACCATGG ACAATGTGGC CACTTTCTGC ATGTTGCTGA TGCTCTTCAT CTTCATATTT
    AGTATCCTAG GGATGCATCT GTTCGGCTGC AAATTTGCCT CCGAGAAAGA TGGCGACACT
    CTACCCGACA GGAAGAATTT TGACTCTCTG CTGTGGGCTA CGGTCACGGT GTTCCAGATC
    CTAACCCAAG AGGACTGGAA CAAGGTTCTG TACAACGGCA TGGCTTCAAC GTCTTCTTGG
    GCTGCCCTCT ACTTCATCGC GCTGATGACG TTTGGAAATT ACGTTCTCTT CAACCTGTTG
    GTGGCCATCC TTGTAGAGGG GTTTCAGACT GAAGAGGTAT CTAAAAGAGA AGACATGAGT
    GCTCATTTAA GTTGCATACA GCTTCCAGTT GATTCCATAG GGCAAGGGGA GGGCTCTCAG
    TCGGGTTCTG AATCTGCCCA CGAGGACTGC CATATCCGCA AGAGTCTTTC CAATGCTTCT
    TTTACTCCTT TACATGGGTA CCCAGATGGA AAGAGTATGT TACAACCACC CCTTATCATC
    CACACAGCAG CCACACCCAT GCCCATGCCA AAATGTGCTT TGTTTGGAGA TATCCCCATG
    GCTGGTGAAT CTAGGAGGGA GAGTAATGTC TCCGGTGGCC CCCATGCTTG TGAAATCAGG
    TCTATGTCAA GTTCAAGGAA CTCCCCGCAC AGCTCCTGGG GTGCAGTGTG TAGTTGGAAT
    AGCCGTAGAT CTAGTTGGAA CAGCATTGGA CGGGCTTCCA GCCTAAAACG GCACGAGCAA
    AGCGGTGAAC ACAAATCTTT GCTGTCTGCT GATGGAAAAG AAAGCTCAGA AGGCGACACA
    TCAGACGAGG AGGTGTCCAG CCGTACCGGC TCTTCCCTCA ACCGGATGGA CCTCGTGGGT
    AAACGGGAGG CGTACGACAT GCACGATACA TTGCATGTGC CTTATTCTTA TGGAATGCAG
    AGCAGACACA GCCTGCCTGG GGAGTACCAA GACTGCAATG GAAAGACATC ACCTGTTTTT
    CCTCCACTCC ATGTAGAAGA ATCCAAGGTT GACTATAGCA ATAACATCGA TGATGATGTA
    AACATGAGTA GGGGAGAAAG ACTGAAAGCT TGGATCATAT CCCATTTACC AGCCTGGTGC
    AAGGAGAGAG AAACTTGGTC GCTGTATTTG TTCCCGCCAT ATTCCAAATT CCGGCTGATG
    TGCAATAAAA TTATCACCCA CAGAATGTTT GATCACGTGG TCTTGGTCAT CATATTTCTC
    AACTGTATAA CCATTGCCAT GGAAAGGCCC AAAATAGACC CCCACAGTGC CGAAAGAATA
    TTTCTCACGT TGTCCAACTA CATATTCACC TTGATTTTCT TGGCTGAAAT GACAGTGAAG
    GTGGTGGCCA TGAACTTTTG CTTTGGTGGG AAGGCGTACC TGAGGAGCAG TTGGAATGTG
    TTGGATGGAA TGCTGGTCTT GATCTCAGTC ATTGACATTC TAGTCTCTAT GGTGTCCGAC
    AGCGGCACCA AGATCCTTGG CATGCTAAGA GTGCTGCGGC TGCTCCGGAC TTTAAGGCCA
    CTCAGGGTGA TCAGTCGAGC TCAGGGACTG AAGCTGGTGG TTGAGACACT AATGTCTTCT
    TTAAAGCCTA TCGGTAACAT TGTGGTGATC TGCTGTGCCT TCTTCATCAT ATTTGGGATA
    CTTGGAGTTC AGCTTTTCAA GGGGAAATTT TATTACTGCC AGGGAGAGGA CACTCGAAAC
    ATCACTAATA AATCCGACTG CCTTGAAAAC AAGTATAAAT GGGCAAGACA CAAGTACAAC
    TTTGATAATC TTGGCCAGGC TCTGATGTCT CTGTTTGTCC TGGCATCTAA AGACGGATGG
    GTGGACATCA TGTACGATGG GCTGGATGCT GTCGGGGTTG ACCAGCAGCC AGTGATGAAC
    TACAACCCTT GGATGCTGCT CTACTTCATC TCGTTTCTGC TGATCGTGGC GTTCTTTGTG
    CTCAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCCGGCA GCATCAGGAG
    GAGGAGGAGG CGAAGAGACG GGAAGAGAAG AGGCTACGCA GGATGGAGAA AAAGAGAAGG
    AAGGCCCAGT GCAAACCCTA CTACTCGGAG TATTCTCACC CTCGGCTGCT CATCCATCAA
    ATGTGCACAA GCCATTATCT GGATTTGTTT ATTACTGGGG TCATCGGATT GAATGTTATC
    ACTATGGCGA TGGAGCACTA CCAGCAACCC AAGGTATTGG ATGAAGCTTT AAAAATCTGT
    AATTACATAT TTACCCTCAT TTTTGTGCTG GAATCTGGGT GCAAAATGGT AGCTTTTGGC
    TTCCGGCGAT TCTTCAAAGA CAGGTGGAAC CAGTTGGATT TGGCCATTGT TCTGCTCTCC
    ATTATGGGGA TCACCCTGGA GGAGATTGAA GTCAATGCAT CGCTGCCCAT TAACCCAACA
    ATCATCAGAA TAATGAGGGT CCTGCGGATC GCTCGCGTGC TGAAGTTATT AAAGATGGCA
    GTCGGTATGC GAGCCCTGCT GGATACAGTA TTGCAAGCGC TTCCTCAAGT GGGGAATCTC
    GGCCTGCTCT TTATGCTGCT GTTTTTCATA TTTGCCGCTT TGGGAGTTGA ACTTTTTGGC
    GACTTAGAAT GCACTGATTC CCACCCGTGT GAAGGTCTCG GCAGGCACGC CACGTTCAGG
    AACTTCGGCA TGGCCTTTCT GACTTTGTTC AGAGTATCCA CAGGAGATAA CTGGAACGGT
    ATAATGAAGG ACACACTCCG TGATTGCAAC GAGGATTCCA CCTGTTACAA CACAGTCATC
    TCCCCCATCT ATTTTGTCTC TTTTGTGCTG ACTGCCCAGT TTGTCCTGGT CAATGTGGTA
    ATAGCGGTGC TCATGAAACA CCTTGAGGAA AGCAACAAGG AAGCTAAGGA GGAGGCGGAG
    CTGGAGGCAG AGATGGAACT AGAGTTTCAG ATCCAGAACA TGCAGGTTCC GGATCCTGAC
    CCATTTATGT GGGCTGGGAG AGATCTCGGC TATCCCCAGG AAGGTTCTTA CGGATATATG
    AACTCCGTGC AGGACAAGGC CGACTCACAG CTTTCATTAG TCAATCCCAT GGAAAGACAC
    ATGTTTGATA CAATATCACT ACTGATCCAG GGATCTTTGG AAGGAGAGAT GAAGCTGATG
    GACAATTTGT CTGGCTCTGT GTGCCACCAT TACGCCCTCC CTCCCTCTGA CAAGGGCTCC
    TCAGAAAAAC AGATCCCATT GGCAGAGATG GAAGGCCTAT CCATGACATC AGACATTCTG
    TCCGAGAAAT CATGGTCCTT AGCTCTGACG GACGACTCCC TGCCAGATGA CCCTAATCTC
    AACCCATTTG GTGTACTGGA AACCAAACTT GGCCTCCCCG AGGAGAAACA TTCGGCAACC
    GTTGAGGAGG CTACGAATCT TCTCAGCGTC CGGAAACCGT CAGTATGCCG CACACATTCC
    CTGCCCAATG ACAGCTACAT GTTGCAAACG GGAAGCACGT GCGCTGAGTG CCAGGAGCAA
    AGAAACCACT GTTACCAGAA ATCAGGTTCC AGCTCAACGG TTCAGTCTCA GCCAAATGAC
    CTCAACTCTC TTCTCCAAGT GCCACAAATC TCCATCCCTT ATGAAGCTTC AGGAAGTACA
    GTGATATCAG AGATTCCCAA ACTCCCCGCC CCCTTGCGCT CTCCATCGGC TAATAGGATG
    CTCCGCAGAC AGGAAGCAAT CAGAACCGAC TCTTTGGAAT TTTTTCCCGA TAGCAAAGAA
    AATGCTGGCA TAACTGAGCC CCCACCTGTA TATATTCCAA TGTGCCAGGT GCCCACCGAG
    ACACCCTCGG CTTTTATAGA ACCAGAAAGA ACTTTATGCC AGTTGAAGCG ACGCAGTGTT
    TACACCCAGC AAGTCCAAGA TACCTGCAAG ATGTTTAGGT ACCCTGATGC TTCTCAAACC
    AACATGGACA CGTTACGAGA AACCTCAAAG ATTCCAGAGA AAGAAGATTT GTTGAGCGCA
    GCATGTTGTA GGCGGCATCT CAACTCCAAA ACGTGTAAAG CATTAAGCGT CTATTCTGCA
    GCATACTCCT TAAGCCAGAA ACTGAAACTA GGTAACAGCG GCAACGTTAT TTTGAAGGAC
    ATGAAGAGGT TCTACAGCGT AGATGCCCAC GGCGTCCTTA AAAAGACATC CTCTTGGTTG
    GATGACCCCA GAAGACATTC CATAGAAATT TGTTCTTTAG AGAACACTCT TCATCACTAC
    TCTACTTCAC GTTCTTGTGG ATTCATTAGC CACGTATTCC AGAAGAAGGA GGACTTGCAA
    ATAGTGCAGA GGACGGGCGG ACAGGATCCC GTCTGTCCAG ATTGTGTTCA CTCAGGAGAA
    GCATCAGAAC TACCACTCTC TTACGTCTCG AGTGGGCAAT CACAATCGAC AGACTCGACC
    GAGCTCGCTA AAATAGACTC TTTCGTTCCG GAAATGCAGA CATCCCCTAA ACCACTGGGA
    GATTTAGTTT CTGTCGAAAG TTTTTCTATG GATGCAGTTG AATTAAATCA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.