plch1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	plch1
Entrez Gene ID	100497252
Full Name	phospholipase C eta 1
General protein information	Preferred Names phospholipase C eta 1 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031902337.1	XP_031758197.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
XM_012963444.2	XP_012818898.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_031902336.1	XP_031758196.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_031902338.1	XP_031758198.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
XM_018094382.1	XP_017949871.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
XM_012963444.3	XP_012818898.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
XM_018094382.2	XP_017949871.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
XM_031902339.1	XP_031758199.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X6

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PLCH1	XP_001149022.1
	LOC707243	XP_002808381.1
Bos taurus (cattle)	PLCH1	XP_002685035.3
Rattus norvegicus (Norway rat)	Plch1	NP_001178636.1
Xenopus tropicalis (tropical clawed frog)	plch1	XP_002933304.2
Homo sapiens (human)	PLCH1	NP_001124432.1
Gallus gallus (chicken)	PLCH1	XP_004943620.1
Danio rerio (zebrafish)	LOC566479	XP_694841.5
Mus musculus (house mouse)	Plch1	NP_899014.2
Canis lupus familiaris (dog)	PLCH1	XP_005634651.1

Gene Ontology

The following plch1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the plch1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa58661	XM_031902339.1 Latest version!	Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa36823	XM_012963444.3 Latest version!	Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa44744	XM_018094382.2 Latest version!	Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa58658	XM_031902336.1 Latest version!	Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa58659	XM_031902337.1 Latest version!	Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa58660	XM_031902338.1 Latest version!	Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OXa36823	XM_012963444.2	Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa44744	XM_018094382.1	Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

plch1 ( XM_031902339.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758199.1 Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa58661
Clone ID Related Accession (Same CDS sequence)	XM_031902339.1
Accession Version	XM_031902339.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCC AAAATCAGGA TAAGAGAAGA TTTCTGCAGT ACTGCCGCCA TTTCTTAGCT 
GACAATGGGG CATTTCATGT TGAAAGATGT ATGAGTGTAA TGCAGTCTGG GACACAGATG 
GTAAAACTAA AGAGTGGAAC AAAGGCTTTG GTTCGCCTCT TTTACCTGGA TGAACATCGG 
ACATGTATCA GATGGAGACC TTCAAGAAAA AGCGAGAAAG CTAAAATTAT AATTGATTCA 
ATTTACAAAG TCACGGAAGG GCGACAGTCT GAAATATTCC ACCGCCATGC AGAAGGAAAC 
TTTGATCCCA GTTGCTGTTT TACCATATAT CATGGAAACC ACATGGAATC TCTGGACCTC 
ATAACATCAA ATCCGGAGGA GGCCCGAACT TGGATCACAG GGCTGAAATA TCTGATGGCT 
GGAATTAGCG ATGAGGATTC TCTGGCAAAA AGGCAAAGGA CCCATGATCA ATGGGTGAAG 
CAGACATTTG AAGAGGCTGA TAAAAATGGG GATGGCTTAC TCAACATTGA GGAAATATAC 
CAGTTAATGC ATAAACTCAA CGTGAATCTG CCACGAAGGA AAGTTCGCCA GATGTTCCAG 
GAAGCAGACA CGGACGAGAA CCAAGGGACT CTGACCTTTG AAGAGTTTTC TGTGTTTTAC 
AAGATGATGT CATTACGGAG GGATTTGTAC CTGTTACTTT TAAACTACAG CGACAAAAAG 
GATCACTTAA CAGTTGACGA TCTGGCTCAG TTTTTCAAAG TAGAACAAAA GATGAATAAT 
GTGACAACAG AATACTGTAT TGACATTATA CAGAAATTTG AAGTCTCAGA GGAAAACAAA 
GAACAACATG TTCTGGGAAT AGATGGTTTT ACTAATTTCA TGAGAAGTCC TGCAGGAGAT 
ATTTTTAACC CTCAGCACTG TGAGATCTAC CAAGACATGG ACCAACCTCT CTGTAGCTAC 
TACATTGCAT CATCCCATAA CACCTACTTA ACTGGAGACC AACTTCTTTC TCAATCCAAA 
GTGGATATGT ATGCTTGGGT ATTGCAAGAT GGCTGTCGAT GCATAGAAGT TGACTGCTGG 
GATGGACCAG ATGGGGAGCC AGTGGTACAC CATGGCTATA CCCTTACTTC CAAAGTCCTT 
TTTAAAGATG TTGTGGAAAC CATCAACAAA CATGCTTTTG TGAAAAATGA ATATCCTGTT 
ATACTTTCAA TTGAAAACCA CTGCAACATT CAACAACAAA GAAAGATTGC TCAGTACATG 
AAAGATATAT TTGGGGATAA ACTTGATCTG TCTTCTGTCC CACTTGGAGA ATCCAAGCAA 
CTTCCTAGTC CTCAGAATCT CAAGGGAAAG ATATTAGTGA AGGGGAAAAA GTTGCCATAT 
GACCTGGCAG ACGATGCTGA GGAGGGAGAA GTCTCAGATG AAGATAGTGC TGACGAAATA 
GAAGAAGAAT GCAAACTCAC ACAATGTTTT AGTAATGGCA CAACAGAGCA CCAGGTGGAA 
TCTTTCATTC GGAAAAAACT GGAGTCTTTG CTAAAGGAAT CCCAGATCAG GGATAAGGAA 
GATCCAGATA GCTTTACCGT GAAAGCATTA CTAAAGGCAA CACATGAAGG CCTGAATGTT 
AATCTTAAAC AGAATGATGA CACAAAAGAT TGTGGAAAGT CTAAAAGGTC TTCAGTTGGA 
AGTATTGCCA AACACAAAAA AGCTTCCAAA TCCAGGTCTA AATCTTGCAG TACTAGTGAT 
GATGAAGATT CATCGCCAAA AGAGACAACA GCACAGGCAC ACAGGTGGAG CCAAGCTACA 
TATGGCATCT TGCTTACTTG TGCTTGGATT GGTGGAGAGG AAGATGTTGA ATATGCAAAT 
TACAGGTTGG GTCGTCGAAG GAAGACAATG AAGTTATGTC GAGCTTTGTC AGACTTGGTT 
GTCTACACAA ATTCTGTGGC TGCACAAGAC ATTGTAGATG ATGGGACCTC AGGAAACGTT 
TTATCCTTTA GTGAAACAAG AGCACACCAG GTGGTTCAGC ACAAATCTGA GCAGCTCATG 
TTGTACAATC AAAAACAATT AACTAGGATT TATCCATCTG CATACAGGAT AGACTCTAGT 
AATTTTAACC CAGTGGCCTA CTGGAATGCA GGAATTCAGC TTGTTGCGTT AAACTATCAA 
ACAGAAGGAC GCATGATGGA ATTAAATCGA GCTAAATTCA AGATGAATGG TGGTTGTGGA 
TATGTCCTTA AGCCCCAGCA GATGTGCAAA GGTGTATTTA ACCAATTTTC AGCTGACCCT 
CTTCCTGCCA GCCCAAAAAA GCAGTTAATT TTGAAGATAA TCAGTGGACA GCAACTTCCC 
AAACCACCAG ATTCAATGCT TGGGGATAGA GGAGAGATCA TTGACCCTTT TGTAGAAGTG 
GAAATTATCG GCTTGCCTGT GGACTGCTGT AAGGAACAGA CTCGAGTAGT TGATGACAAT 
GGTTTCAATC CTGTATGGGA GGAAACTCTT ACCTTTACAG TTCATATGCC AGAAATAGCA 
TTGGTAAGGT TCTTAGTATG GGACCATGAT CCAATTGGCC GGGATTTTGT TGGACAAAGG 
ACTGTAGCCT TTAACAGTCT TATGCCTGGA TATCGGCATG TTTATCTGGA TGGTCTAACA 
GAAGCCTCCA TATTTGTTCA TGTTACAATT AATGAAATAT ATGGAAAGTG GAGTCCTTTA 
ATCCTCAATC CCAGTTACAC AATATTGCAC TTTCTAGGAG CTACGAAGAA CAGGCAGTTT 
ATTGGCCTGA AAGGGTTTTT CAATAGGAGT TCTAAGCATA GCTCTGTTGA GAGCAATAGT 
CATGTTCGTA AGAAATCAAT TGGGGACCGT ATCTTGCGAC GCAGTGCGAG TGCTCCAGCC 
AAAGCCAGGA AGAAGACCAA GATGGTGTTT CAAGAGTCAA CTGAGAGTAA AGACATTGTC 
TGTGAAACAT CTGGTGCTAA AGAGAGAGAT TTTATGAGAA AGAGAACCTC AAGGAGTCTG 
CAAGCACGCC CTGTTTCAAT GCCTGTGGAT AAGTTACTAC TTGGGGCTTT TGAACCTTCA 
ACATCAGAGA TTTCTAAGGA GACTAGAAGT GACATAGCTT CAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758199.1
CDS	254..3361
Translation	MSSQNQDKRRFLQYCRHFLADNGAFHVERCMSVMQSGTQMVKLKSGTKALVRLFYLDEHRTCIRWRPSRKSEKAKIIIDSIYKVTEGRQSEIFHRHAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIYQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFSVFYKMMSLRRDLYLLLLNYSDKKDHLTVDDLAQFFKVEQKMNNVTTEYCIDIIQKFEVSEENKEQHVLGIDGFTNFMRSPAGDIFNPQHCEIYQDMDQPLCSYYIASSHNTYLTGDQLLSQSKVDMYAWVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKVLFKDVVETINKHAFVKNEYPVILSIENHCNIQQQRKIAQYMKDIFGDKLDLSSVPLGESKQLPSPQNLKGKILVKGKKLPYDLADDAEEGEVSDEDSADEIEEECKLTQCFSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVKALLKATHEGLNVNLKQNDDTKDCGKSKRSSVGSIAKHKKASKSRSKSCSTSDDEDSSPKETTAQAHRWSQATYGILLTCAWIGGEEDVEYANYRLGRRRKTMKLCRALSDLVVYTNSVAAQDIVDDGTSGNVLSFSETRAHQVVQHKSEQLMLYNQKQLTRIYPSAYRIDSSNFNPVAYWNAGIQLVALNYQTEGRMMELNRAKFKMNGGCGYVLKPQQMCKGVFNQFSADPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKEQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVAFNSLMPGYRHVYLDGLTEASIFVHVTINEIYGKWSPLILNPSYTILHFLGATKNRQFIGLKGFFNRSSKHSSVESNSHVRKKSIGDRILRRSASAPAKARKKTKMVFQESTESKDIVCETSGAKERDFMRKRTSRSLQARPVSMPVDKLLLGAFEPSTSEISKETRSDIASV

Target ORF information:

RefSeq Version	XM_031902339.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902339.1

ORF Insert Sequence:

ATGAGTTCCC AAAATCAGGA TAAGAGAAGA TTTCTGCAGT ACTGCCGCCA TTTCTTAGCT 
GACAATGGGG CATTTCATGT TGAAAGATGT ATGAGTGTAA TGCAGTCTGG GACACAGATG 
GTAAAACTAA AGAGTGGAAC AAAGGCTTTG GTTCGCCTCT TTTACCTGGA TGAACATCGG 
ACATGTATCA GATGGAGACC TTCAAGAAAA AGCGAGAAAG CTAAAATTAT AATTGATTCA 
ATTTACAAAG TCACGGAAGG GCGACAGTCT GAAATATTCC ACCGCCATGC AGAAGGAAAC 
TTTGATCCCA GTTGCTGTTT TACCATATAT CATGGAAACC ACATGGAATC TCTGGACCTC 
ATAACATCAA ATCCGGAGGA GGCCCGAACT TGGATCACAG GGCTGAAATA TCTGATGGCT 
GGAATTAGCG ATGAGGATTC TCTGGCAAAA AGGCAAAGGA CCCATGATCA ATGGGTGAAG 
CAGACATTTG AAGAGGCTGA TAAAAATGGG GATGGCTTAC TCAACATTGA GGAAATATAC 
CAGTTAATGC ATAAACTCAA CGTGAATCTG CCACGAAGGA AAGTTCGCCA GATGTTCCAG 
GAAGCAGACA CGGACGAGAA CCAAGGGACT CTGACCTTTG AAGAGTTTTC TGTGTTTTAC 
AAGATGATGT CATTACGGAG GGATTTGTAC CTGTTACTTT TAAACTACAG CGACAAAAAG 
GATCACTTAA CAGTTGACGA TCTGGCTCAG TTTTTCAAAG TAGAACAAAA GATGAATAAT 
GTGACAACAG AATACTGTAT TGACATTATA CAGAAATTTG AAGTCTCAGA GGAAAACAAA 
GAACAACATG TTCTGGGAAT AGATGGTTTT ACTAATTTCA TGAGAAGTCC TGCAGGAGAT 
ATTTTTAACC CTCAGCACTG TGAGATCTAC CAAGACATGG ACCAACCTCT CTGTAGCTAC 
TACATTGCAT CATCCCATAA CACCTACTTA ACTGGAGACC AACTTCTTTC TCAATCCAAA 
GTGGATATGT ATGCTTGGGT ATTGCAAGAT GGCTGTCGAT GCATAGAAGT TGACTGCTGG 
GATGGACCAG ATGGGGAGCC AGTGGTACAC CATGGCTATA CCCTTACTTC CAAAGTCCTT 
TTTAAAGATG TTGTGGAAAC CATCAACAAA CATGCTTTTG TGAAAAATGA ATATCCTGTT 
ATACTTTCAA TTGAAAACCA CTGCAACATT CAACAACAAA GAAAGATTGC TCAGTACATG 
AAAGATATAT TTGGGGATAA ACTTGATCTG TCTTCTGTCC CACTTGGAGA ATCCAAGCAA 
CTTCCTAGTC CTCAGAATCT CAAGGGAAAG ATATTAGTGA AGGGGAAAAA GTTGCCATAT 
GACCTGGCAG ACGATGCTGA GGAGGGAGAA GTCTCAGATG AAGATAGTGC TGACGAAATA 
GAAGAAGAAT GCAAACTCAC ACAATGTTTT AGTAATGGCA CAACAGAGCA CCAGGTGGAA 
TCTTTCATTC GGAAAAAACT GGAGTCTTTG CTAAAGGAAT CCCAGATCAG GGATAAGGAA 
GATCCAGATA GCTTTACCGT GAAAGCATTA CTAAAGGCAA CACATGAAGG CCTGAATGTT 
AATCTTAAAC AGAATGATGA CACAAAAGAT TGTGGAAAGT CTAAAAGGTC TTCAGTTGGA 
AGTATTGCCA AACACAAAAA AGCTTCCAAA TCCAGGTCTA AATCTTGCAG TACTAGTGAT 
GATGAAGATT CATCGCCAAA AGAGACAACA GCACAGGCAC ACAGGTGGAG CCAAGCTACA 
TATGGCATCT TGCTTACTTG TGCTTGGATT GGTGGAGAGG AAGATGTTGA ATATGCAAAT 
TACAGGTTGG GTCGTCGAAG GAAGACAATG AAGTTATGTC GAGCTTTGTC AGACTTGGTT 
GTCTACACAA ATTCTGTGGC TGCACAAGAC ATTGTAGATG ATGGGACCTC AGGAAACGTT 
TTATCCTTTA GTGAAACAAG AGCACACCAG GTGGTTCAGC ACAAATCTGA GCAGCTCATG 
TTGTACAATC AAAAACAATT AACTAGGATT TATCCATCTG CATACAGGAT AGACTCTAGT 
AATTTTAACC CAGTGGCCTA CTGGAATGCA GGAATTCAGC TTGTTGCGTT AAACTATCAA 
ACAGAAGGAC GCATGATGGA ATTAAATCGA GCTAAATTCA AGATGAATGG TGGTTGTGGA 
TATGTCCTTA AGCCCCAGCA GATGTGCAAA GGTGTATTTA ACCAATTTTC AGCTGACCCT 
CTTCCTGCCA GCCCAAAAAA GCAGTTAATT TTGAAGATAA TCAGTGGACA GCAACTTCCC 
AAACCACCAG ATTCAATGCT TGGGGATAGA GGAGAGATCA TTGACCCTTT TGTAGAAGTG 
GAAATTATCG GCTTGCCTGT GGACTGCTGT AAGGAACAGA CTCGAGTAGT TGATGACAAT 
GGTTTCAATC CTGTATGGGA GGAAACTCTT ACCTTTACAG TTCATATGCC AGAAATAGCA 
TTGGTAAGGT TCTTAGTATG GGACCATGAT CCAATTGGCC GGGATTTTGT TGGACAAAGG 
ACTGTAGCCT TTAACAGTCT TATGCCTGGA TATCGGCATG TTTATCTGGA TGGTCTAACA 
GAAGCCTCCA TATTTGTTCA TGTTACAATT AATGAAATAT ATGGAAAGTG GAGTCCTTTA 
ATCCTCAATC CCAGTTACAC AATATTGCAC TTTCTAGGAG CTACGAAGAA CAGGCAGTTT 
ATTGGCCTGA AAGGGTTTTT CAATAGGAGT TCTAAGCATA GCTCTGTTGA GAGCAATAGT 
CATGTTCGTA AGAAATCAAT TGGGGACCGT ATCTTGCGAC GCAGTGCGAG TGCTCCAGCC 
AAAGCCAGGA AGAAGACCAA GATGGTGTTT CAAGAGTCAA CTGAGAGTAA AGACATTGTC 
TGTGAAACAT CTGGTGCTAA AGAGAGAGAT TTTATGAGAA AGAGAACCTC AAGGAGTCTG 
CAAGCACGCC CTGTTTCAAT GCCTGTGGAT AAGTTACTAC TTGGGGCTTT TGAACCTTCA 
ACATCAGAGA TTTCTAAGGA GACTAGAAGT GACATAGCTT CAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plch1 ( XM_012963444.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818898.1 Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa36823
Clone ID Related Accession (Same CDS sequence)	XM_012963444.2 , XM_012963444.3
Accession Version	XM_012963444.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963444.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCC AAAATCAGGA TAAGAGAAGA TTTCTGCAGT ACTGCCGCCA TTTCTTAGCT 
GACAATGGGG CATTTCATGT TGAAAGATGT ATGAGTGTAA TGCAGTCTGG GACACAGATG 
GTAAAACTAA AGAGTGGAAC AAAGGCTTTG GTTCGCCTCT TTTACCTGGA TGAACATCGG 
ACATGTATCA GATGGAGACC TTCAAGAAAA AGCGAGAAAG CTAAAATTAT AATTGATTCA 
ATTTACAAAG TCACGGAAGG GCGACAGTCT GAAATATTCC ACCGCCATGC AGAAGGAAAC 
TTTGATCCCA GTTGCTGTTT TACCATATAT CATGGAAACC ACATGGAATC TCTGGACCTC 
ATAACATCAA ATCCGGAGGA GGCCCGAACT TGGATCACAG GGCTGAAATA TCTGATGGCT 
GGAATTAGCG ATGAGGATTC TCTGGCAAAA AGGCAAAGGA CCCATGATCA ATGGGTGAAG 
CAGACATTTG AAGAGGCTGA TAAAAATGGG GATGGCTTAC TCAACATTGA GGAAATATAC 
CAGTTAATGC ATAAACTCAA CGTGAATCTG CCACGAAGGA AAGTTCGCCA GATGTTCCAG 
GAAGCAGACA CGGACGAGAA CCAAGGGACT CTGACCTTTG AAGAGTTTTC TGTGTTTTAC 
AAGATGATGT CATTACGGAG GGATTTGTAC CTGTTACTTT TAAACTACAG CGACAAAAAG 
GATCACTTAA CAGTTGACGA TCTGGCTCAG TTTTTCAAAG TAGAACAAAA GATGAATAAT 
GTGACAACAG AATACTGTAT TGACATTATA CAGAAATTTG AAGTCTCAGA GGAAAACAAA 
GAACAACATG TTCTGGGAAT AGATGGTTTT ACTAATTTCA TGAGAAGTCC TGCAGGAGAT 
ATTTTTAACC CTCAGCACTG TGAGATCTAC CAAGACATGG ACCAACCTCT CTGTAGCTAC 
TACATTGCAT CATCCCATAA CACCTACTTA ACTGGAGACC AACTTCTTTC TCAATCCAAA 
GTGGATATGT ATGCTTGGGT ATTGCAAGAT GGCTGTCGAT GCATAGAAGT TGACTGCTGG 
GATGGACCAG ATGGGGAGCC AGTGGTACAC CATGGCTATA CCCTTACTTC CAAAGTCCTT 
TTTAAAGATG TTGTGGAAAC CATCAACAAA CATGCTTTTG TGAAAAATGA ATATCCTGTT 
ATACTTTCAA TTGAAAACCA CTGCAACATT CAACAACAAA GAAAGATTGC TCAGTACATG 
AAAGATATAT TTGGGGATAA ACTTGATCTG TCTTCTGTCC CACTTGGAGA ATCCAAGCAA 
CTTCCTAGTC CTCAGAATCT CAAGGGAAAG ATATTAGTGA AGGGGAAAAA GTTGCCATAT 
GACCTGGCAG ACGATGCTGA GGAGGGAGAA GTCTCAGATG AAGATAGTGC TGACGAAATA 
GAAGAAGAAT GCAAACTCAC ACAATGTTTT AGTAATGGCA CAACAGAGCA CCAGGTGGAA 
TCTTTCATTC GGAAAAAACT GGAGTCTTTG CTAAAGGAAT CCCAGATCAG GGATAAGGAA 
GATCCAGATA GCTTTACCGT GAAAGCATTA CTAAAGGCAA CACATGAAGG CCTGAATGTT 
AATCTTAAAC AGAATGATGA CACAAAAGAT TGTGGAAAGT CTAAAAGGTC TTCAGTTGGA 
AGTATTGCCA AACACAAAAA AGCTTCCAAA TCCAGGTCTA AATCTTGCAG TACTAGTGAT 
GATGAAGATT CATCGCCAAA AGAGACAACA GCACAGGCAC ACAGGTTGGG TCGTCGAAGG 
AAGACAATGA AGTTATGTCG AGCTTTGTCA GACTTGGTTG TCTACACAAA TTCTGTGGCT 
GCACAAGACA TTGTAGATGA TGGGACCTCA GGAAACGTTT TATCCTTTAG TGAAACAAGA 
GCACACCAGG TGGTTCAGCA CAAATCTGAG CAGCTCATGT TGTACAATCA AAAACAATTA 
ACTAGGATTT ATCCATCTGC ATACAGGATA GACTCTAGTA ATTTTAACCC AGTGGCCTAC 
TGGAATGCAG GAATTCAGCT TGTTGCGTTA AACTATCAAA CAGAAGGACG CATGATGGAA 
TTAAATCGAG CTAAATTCAA GATGAATGGT GGTTGTGGAT ATGTCCTTAA GCCCCAGCAG 
ATGTGCAAAG GTGTATTTAA CCAATTTTCA GCTGACCCTC TTCCTGCCAG CCCAAAAAAG 
CAGTTAATTT TGAAGATAAT CAGTGGACAG CAACTTCCCA AACCACCAGA TTCAATGCTT 
GGGGATAGAG GAGAGATCAT TGACCCTTTT GTAGAAGTGG AAATTATCGG CTTGCCTGTG 
GACTGCTGTA AGGAACAGAC TCGAGTAGTT GATGACAATG GTTTCAATCC TGTATGGGAG 
GAAACTCTTA CCTTTACAGT TCATATGCCA GAAATAGCAT TGGTAAGGTT CTTAGTATGG 
GACCATGATC CAATTGGCCG GGATTTTGTT GGACAAAGGA CTGTAGCCTT TAACAGTCTT 
ATGCCTGGAT ATCGGCATGT TTATCTGGAT GGTCTAACAG AAGCCTCCAT ATTTGTTCAT 
GTTACAATTA ATGAAATATA TGGAAAGTGG AGTCCTTTAA TCCTCAATCC CAGTTACACA 
ATATTGCACT TTCTAGGAGC TACGAAGAAC AGGCAGTTTA TTGGCCTGAA AGGGTTTTTC 
AATAGGAGTT CTAAGCATAG CTCTGTTGAG AGCAATAGTC ATGTTCGTAA GAAATCAATT 
GGGGACCGTA TCTTGCGACG CAGTGCGAGT GCTCCAGCCA AAGCCAGGAA GAAGACCAAG 
ATGGTGTTTC AAGAGTCAAC TGAGAGTAAA GACATTGTCT GTGAAACATC TGGTGCTAAA 
GAGAGAGATT TTATGAGAAA GAGAACCTCA AGGAGTCTGC AAGCACGCCC TGTTTCAATG 
CCTGTGGATA AGTTACTACT TGGGGCTTTT GAACCTTCAA CATCAGAGAT TTCTAAGGAG 
ACTAGAAGTG ACATAGCTTC AGGTTCAGCA GGGGAGATGC AAGCTAAGCA CCAGGAGAAG 
CCAGAGATGA CAAAACTGGA TCTACCAGTT CATAAAAATA ATCTTGCTAT TCAACATTCT 
TCCCCTCTTT TTAAAGATTG TCACCAGACT GAAAATCCAG TAAATAAACC TGTACATAAA 
AAATGGAGGA GCTTACATTT AGAAAAACAA AACTATGATG ATATTAGAGA GTTAAATGAT 
ACATTTACCA GTGAATCTGA TGAGGAGATT TTAAAGCCAA AAGAAGGTAA TGAAAAAGAA 
GCTATGCTGT TATTAGACAA AATGGAGTCC ATCCCAGAAA ACACCAAGCA TCATGCAAAA 
GCTGATTTAT TTTCTGTGAC TGATTTATCA AGCACTATAT GTACAGACAT GAATCACTTA 
CAGTCTACTG CACCTTTATT AGAAACTGAT ATTTCTCATA TTATAAGTGA AGTTCCTTCA 
GTCAACGAGA GTGAACTTGG AAGCACGGTG TCTGCTTTAA TAAGGCACTT TGAGATCACC 
GAAGACAAAA CTAATTTGAC ATTTGTCTCA AGTTTGCAAG GTCCAAACAG CCAAAGCAAT 
TTTTGTGACA ATTCTGATGT GTTAATATCT GACCTTTTAA ATAGAAAAAA GCACACAAGA 
CAATCTTTAC ATTCAGCTTC CAACACTACT GTGTTCTCCA GCCCTGAAAC AAGTGAATAT 
TCTATATATA CATTTATTCA TGAAGAGCAT GTACCTCCTG ATGCCAATTA CAAATTGATG 
GATGCAAACA ATGGCATATC ATCGTGTTCT AATTTAAAAA ATAATTCATC AAACAGTTGG 
CTAGATAGTT CTGAGAGCCT ACAGTTTTGT TCTCAATATA ATGCAAACAG CAATACATTA 
GGTCTAAAAT CAAAGCAAAG TAGGCCACAA GTTTCACTGA AAACATCATC AGGCTGTACA 
GATGAGAATG TCAAACAGAT ATCATCTCAC AACTCCTTTC TAAAGAACCT TAATGTAGAT 
AGTAATCCTA AGAGAATAGC GGATAAGAAT TTCTTACATA CTGGTGTCAA TAGGTATGCC 
ACACAAGCCT GTCCAAACTC TCAAAACACA GGCATTCAAC ACGGATCTGG TCAACTTTAT 
CAAAACAGGC ATTCAAGTTA TAAACCAAGA GCAGATGCAC CACGAGACAA ACAGTATATT 
GCCCAGTCTG GAAACCTGCA CAGCTCGCCC AATGCAAACT GGCCCAAACC TTCAAACTAT 
CACATTCATT CATTACAAGG TATTAAACAG CCAAGTCCAT GTATATCAAA AAGTCTTGGA 
GATCTGACAT CAGACGATAT ATCTTGTAAT TTTGAAAGTA AGTACAAACG GATCAACAGA 
GGGTTTCTTT CATCAGCTAT TTGTCCGGAA AATACATCAT TAGCTCAAAG CAGACCTCAG 
TCTACCGATG CTTTAACGGA ACAGTTGCGC AAACTGATGT CTTTTGACCA AGACGACAGT 
AACAGGCCAT CATATTATGA AGAAGAAGAA TGGGACAGTC CTAAGCCACT TACAAGAAAG 
CTATCCTCAA GGAGTCAAAG TAGGGTCAGA AACATTGCTA ACCGTGCCAA AGAAAGACAG 
GAGGCCAATA AAATAAAACA AAGCAATTTA AACTCAAGTG AAGTAGTCTT AAGAAACAAA 
CGTTCCATGG CTTCTCAGCC CATCAACAGG CATTCCACTG GTTCTTATAT AGGTAGCTTG 
TATAATAACT TTAACAGTGA AAGGCTAGAA GGCAGAGGTA TACCAGAAGG TGCATGCACT 
TCCCATACAC AAGTGTATAA TGACCACATT GACACTGGCT TTACTTATCG ACATGAGCCA 
AGCAATACTG ATGAACCAGA AATTTATTTC CTACTAAGAC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818898.1
CDS	249..5153
Translation	MSSQNQDKRRFLQYCRHFLADNGAFHVERCMSVMQSGTQMVKLKSGTKALVRLFYLDEHRTCIRWRPSRKSEKAKIIIDSIYKVTEGRQSEIFHRHAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIYQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFSVFYKMMSLRRDLYLLLLNYSDKKDHLTVDDLAQFFKVEQKMNNVTTEYCIDIIQKFEVSEENKEQHVLGIDGFTNFMRSPAGDIFNPQHCEIYQDMDQPLCSYYIASSHNTYLTGDQLLSQSKVDMYAWVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKVLFKDVVETINKHAFVKNEYPVILSIENHCNIQQQRKIAQYMKDIFGDKLDLSSVPLGESKQLPSPQNLKGKILVKGKKLPYDLADDAEEGEVSDEDSADEIEEECKLTQCFSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVKALLKATHEGLNVNLKQNDDTKDCGKSKRSSVGSIAKHKKASKSRSKSCSTSDDEDSSPKETTAQAHRLGRRRKTMKLCRALSDLVVYTNSVAAQDIVDDGTSGNVLSFSETRAHQVVQHKSEQLMLYNQKQLTRIYPSAYRIDSSNFNPVAYWNAGIQLVALNYQTEGRMMELNRAKFKMNGGCGYVLKPQQMCKGVFNQFSADPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKEQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVAFNSLMPGYRHVYLDGLTEASIFVHVTINEIYGKWSPLILNPSYTILHFLGATKNRQFIGLKGFFNRSSKHSSVESNSHVRKKSIGDRILRRSASAPAKARKKTKMVFQESTESKDIVCETSGAKERDFMRKRTSRSLQARPVSMPVDKLLLGAFEPSTSEISKETRSDIASGSAGEMQAKHQEKPEMTKLDLPVHKNNLAIQHSSPLFKDCHQTENPVNKPVHKKWRSLHLEKQNYDDIRELNDTFTSESDEEILKPKEGNEKEAMLLLDKMESIPENTKHHAKADLFSVTDLSSTICTDMNHLQSTAPLLETDISHIISEVPSVNESELGSTVSALIRHFEITEDKTNLTFVSSLQGPNSQSNFCDNSDVLISDLLNRKKHTRQSLHSASNTTVFSSPETSEYSIYTFIHEEHVPPDANYKLMDANNGISSCSNLKNNSSNSWLDSSESLQFCSQYNANSNTLGLKSKQSRPQVSLKTSSGCTDENVKQISSHNSFLKNLNVDSNPKRIADKNFLHTGVNRYATQACPNSQNTGIQHGSGQLYQNRHSSYKPRADAPRDKQYIAQSGNLHSSPNANWPKPSNYHIHSLQGIKQPSPCISKSLGDLTSDDISCNFESKYKRINRGFLSSAICPENTSLAQSRPQSTDALTEQLRKLMSFDQDDSNRPSYYEEEEWDSPKPLTRKLSSRSQSRVRNIANRAKERQEANKIKQSNLNSSEVVLRNKRSMASQPINRHSTGSYIGSLYNNFNSERLEGRGIPEGACTSHTQVYNDHIDTGFTYRHEPSNTDEPEIYFLLRL

Target ORF information:

RefSeq Version	XM_012963444.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963444.3

ORF Insert Sequence:

ATGAGTTCCC AAAATCAGGA TAAGAGAAGA TTTCTGCAGT ACTGCCGCCA TTTCTTAGCT 
GACAATGGGG CATTTCATGT TGAAAGATGT ATGAGTGTAA TGCAGTCTGG GACACAGATG 
GTAAAACTAA AGAGTGGAAC AAAGGCTTTG GTTCGCCTCT TTTACCTGGA TGAACATCGG 
ACATGTATCA GATGGAGACC TTCAAGAAAA AGCGAGAAAG CTAAAATTAT AATTGATTCA 
ATTTACAAAG TCACGGAAGG GCGACAGTCT GAAATATTCC ACCGCCATGC AGAAGGAAAC 
TTTGATCCCA GTTGCTGTTT TACCATATAT CATGGAAACC ACATGGAATC TCTGGACCTC 
ATAACATCAA ATCCGGAGGA GGCCCGAACT TGGATCACAG GGCTGAAATA TCTGATGGCT 
GGAATTAGCG ATGAGGATTC TCTGGCAAAA AGGCAAAGGA CCCATGATCA ATGGGTGAAG 
CAGACATTTG AAGAGGCTGA TAAAAATGGG GATGGCTTAC TCAACATTGA GGAAATATAC 
CAGTTAATGC ATAAACTCAA CGTGAATCTG CCACGAAGGA AAGTTCGCCA GATGTTCCAG 
GAAGCAGACA CGGACGAGAA CCAAGGGACT CTGACCTTTG AAGAGTTTTC TGTGTTTTAC 
AAGATGATGT CATTACGGAG GGATTTGTAC CTGTTACTTT TAAACTACAG CGACAAAAAG 
GATCACTTAA CAGTTGACGA TCTGGCTCAG TTTTTCAAAG TAGAACAAAA GATGAATAAT 
GTGACAACAG AATACTGTAT TGACATTATA CAGAAATTTG AAGTCTCAGA GGAAAACAAA 
GAACAACATG TTCTGGGAAT AGATGGTTTT ACTAATTTCA TGAGAAGTCC TGCAGGAGAT 
ATTTTTAACC CTCAGCACTG TGAGATCTAC CAAGACATGG ACCAACCTCT CTGTAGCTAC 
TACATTGCAT CATCCCATAA CACCTACTTA ACTGGAGACC AACTTCTTTC TCAATCCAAA 
GTGGATATGT ATGCTTGGGT ATTGCAAGAT GGCTGTCGAT GCATAGAAGT TGACTGCTGG 
GATGGACCAG ATGGGGAGCC AGTGGTACAC CATGGCTATA CCCTTACTTC CAAAGTCCTT 
TTTAAAGATG TTGTGGAAAC CATCAACAAA CATGCTTTTG TGAAAAATGA ATATCCTGTT 
ATACTTTCAA TTGAAAACCA CTGCAACATT CAACAACAAA GAAAGATTGC TCAGTACATG 
AAAGATATAT TTGGGGATAA ACTTGATCTG TCTTCTGTCC CACTTGGAGA ATCCAAGCAA 
CTTCCTAGTC CTCAGAATCT CAAGGGAAAG ATATTAGTGA AGGGGAAAAA GTTGCCATAT 
GACCTGGCAG ACGATGCTGA GGAGGGAGAA GTCTCAGATG AAGATAGTGC TGACGAAATA 
GAAGAAGAAT GCAAACTCAC ACAATGTTTT AGTAATGGCA CAACAGAGCA CCAGGTGGAA 
TCTTTCATTC GGAAAAAACT GGAGTCTTTG CTAAAGGAAT CCCAGATCAG GGATAAGGAA 
GATCCAGATA GCTTTACCGT GAAAGCATTA CTAAAGGCAA CACATGAAGG CCTGAATGTT 
AATCTTAAAC AGAATGATGA CACAAAAGAT TGTGGAAAGT CTAAAAGGTC TTCAGTTGGA 
AGTATTGCCA AACACAAAAA AGCTTCCAAA TCCAGGTCTA AATCTTGCAG TACTAGTGAT 
GATGAAGATT CATCGCCAAA AGAGACAACA GCACAGGCAC ACAGGTTGGG TCGTCGAAGG 
AAGACAATGA AGTTATGTCG AGCTTTGTCA GACTTGGTTG TCTACACAAA TTCTGTGGCT 
GCACAAGACA TTGTAGATGA TGGGACCTCA GGAAACGTTT TATCCTTTAG TGAAACAAGA 
GCACACCAGG TGGTTCAGCA CAAATCTGAG CAGCTCATGT TGTACAATCA AAAACAATTA 
ACTAGGATTT ATCCATCTGC ATACAGGATA GACTCTAGTA ATTTTAACCC AGTGGCCTAC 
TGGAATGCAG GAATTCAGCT TGTTGCGTTA AACTATCAAA CAGAAGGACG CATGATGGAA 
TTAAATCGAG CTAAATTCAA GATGAATGGT GGTTGTGGAT ATGTCCTTAA GCCCCAGCAG 
ATGTGCAAAG GTGTATTTAA CCAATTTTCA GCTGACCCTC TTCCTGCCAG CCCAAAAAAG 
CAGTTAATTT TGAAGATAAT CAGTGGACAG CAACTTCCCA AACCACCAGA TTCAATGCTT 
GGGGATAGAG GAGAGATCAT TGACCCTTTT GTAGAAGTGG AAATTATCGG CTTGCCTGTG 
GACTGCTGTA AGGAACAGAC TCGAGTAGTT GATGACAATG GTTTCAATCC TGTATGGGAG 
GAAACTCTTA CCTTTACAGT TCATATGCCA GAAATAGCAT TGGTAAGGTT CTTAGTATGG 
GACCATGATC CAATTGGCCG GGATTTTGTT GGACAAAGGA CTGTAGCCTT TAACAGTCTT 
ATGCCTGGAT ATCGGCATGT TTATCTGGAT GGTCTAACAG AAGCCTCCAT ATTTGTTCAT 
GTTACAATTA ATGAAATATA TGGAAAGTGG AGTCCTTTAA TCCTCAATCC CAGTTACACA 
ATATTGCACT TTCTAGGAGC TACGAAGAAC AGGCAGTTTA TTGGCCTGAA AGGGTTTTTC 
AATAGGAGTT CTAAGCATAG CTCTGTTGAG AGCAATAGTC ATGTTCGTAA GAAATCAATT 
GGGGACCGTA TCTTGCGACG CAGTGCGAGT GCTCCAGCCA AAGCCAGGAA GAAGACCAAG 
ATGGTGTTTC AAGAGTCAAC TGAGAGTAAA GACATTGTCT GTGAAACATC TGGTGCTAAA 
GAGAGAGATT TTATGAGAAA GAGAACCTCA AGGAGTCTGC AAGCACGCCC TGTTTCAATG 
CCTGTGGATA AGTTACTACT TGGGGCTTTT GAACCTTCAA CATCAGAGAT TTCTAAGGAG 
ACTAGAAGTG ACATAGCTTC AGGTTCAGCA GGGGAGATGC AAGCTAAGCA CCAGGAGAAG 
CCAGAGATGA CAAAACTGGA TCTACCAGTT CATAAAAATA ATCTTGCTAT TCAACATTCT 
TCCCCTCTTT TTAAAGATTG TCACCAGACT GAAAATCCAG TAAATAAACC TGTACATAAA 
AAATGGAGGA GCTTACATTT AGAAAAACAA AACTATGATG ATATTAGAGA GTTAAATGAT 
ACATTTACCA GTGAATCTGA TGAGGAGATT TTAAAGCCAA AAGAAGGTAA TGAAAAAGAA 
GCTATGCTGT TATTAGACAA AATGGAGTCC ATCCCAGAAA ACACCAAGCA TCATGCAAAA 
GCTGATTTAT TTTCTGTGAC TGATTTATCA AGCACTATAT GTACAGACAT GAATCACTTA 
CAGTCTACTG CACCTTTATT AGAAACTGAT ATTTCTCATA TTATAAGTGA AGTTCCTTCA 
GTCAACGAGA GTGAACTTGG AAGCACGGTG TCTGCTTTAA TAAGGCACTT TGAGATCACC 
GAAGACAAAA CTAATTTGAC ATTTGTCTCA AGTTTGCAAG GTCCAAACAG CCAAAGCAAT 
TTTTGTGACA ATTCTGATGT GTTAATATCT GACCTTTTAA ATAGAAAAAA GCACACAAGA 
CAATCTTTAC ATTCAGCTTC CAACACTACT GTGTTCTCCA GCCCTGAAAC AAGTGAATAT 
TCTATATATA CATTTATTCA TGAAGAGCAT GTACCTCCTG ATGCCAATTA CAAATTGATG 
GATGCAAACA ATGGCATATC ATCGTGTTCT AATTTAAAAA ATAATTCATC AAACAGTTGG 
CTAGATAGTT CTGAGAGCCT ACAGTTTTGT TCTCAATATA ATGCAAACAG CAATACATTA 
GGTCTAAAAT CAAAGCAAAG TAGGCCACAA GTTTCACTGA AAACATCATC AGGCTGTACA 
GATGAGAATG TCAAACAGAT ATCATCTCAC AACTCCTTTC TAAAGAACCT TAATGTAGAT 
AGTAATCCTA AGAGAATAGC GGATAAGAAT TTCTTACATA CTGGTGTCAA TAGGTATGCC 
ACACAAGCCT GTCCAAACTC TCAAAACACA GGCATTCAAC ACGGATCTGG TCAACTTTAT 
CAAAACAGGC ATTCAAGTTA TAAACCAAGA GCAGATGCAC CACGAGACAA ACAGTATATT 
GCCCAGTCTG GAAACCTGCA CAGCTCGCCC AATGCAAACT GGCCCAAACC TTCAAACTAT 
CACATTCATT CATTACAAGG TATTAAACAG CCAAGTCCAT GTATATCAAA AAGTCTTGGA 
GATCTGACAT CAGACGATAT ATCTTGTAAT TTTGAAAGTA AGTACAAACG GATCAACAGA 
GGGTTTCTTT CATCAGCTAT TTGTCCGGAA AATACATCAT TAGCTCAAAG CAGACCTCAG 
TCTACCGATG CTTTAACGGA ACAGTTGCGC AAACTGATGT CTTTTGACCA AGACGACAGT 
AACAGGCCAT CATATTATGA AGAAGAAGAA TGGGACAGTC CTAAGCCACT TACAAGAAAG 
CTATCCTCAA GGAGTCAAAG TAGGGTCAGA AACATTGCTA ACCGTGCCAA AGAAAGACAG 
GAGGCCAATA AAATAAAACA AAGCAATTTA AACTCAAGTG AAGTAGTCTT AAGAAACAAA 
CGTTCCATGG CTTCTCAGCC CATCAACAGG CATTCCACTG GTTCTTATAT AGGTAGCTTG 
TATAATAACT TTAACAGTGA AAGGCTAGAA GGCAGAGGTA TACCAGAAGG TGCATGCACT 
TCCCATACAC AAGTGTATAA TGACCACATT GACACTGGCT TTACTTATCG ACATGAGCCA 
AGCAATACTG ATGAACCAGA AATTTATTTC CTACTAAGAC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plch1 ( XM_018094382.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949871.1 Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X2, mRNA.

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CloneID	OXa44744
Clone ID Related Accession (Same CDS sequence)	XM_018094382.1 , XM_018094382.2
Accession Version	XM_018094382.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCC AAAATCAGGA TAAGAGAAGA TTTCTGCAGT ACTGCCGCCA TTTCTTAGCT 
GACAATGGGG CATTTCATGT TGAAAGATGT ATGAGTGTAA TGCAGTCTGG GACACAGATG 
GTAAAACTAA AGAGTGGAAC AAAGGCTTTG GTTCGCCTCT TTTACCTGGA TGAACATCGG 
ACATGTATCA GATGGAGACC TTCAAGAAAA AGCGAGAAAG CTAAAATTAT AATTGATTCA 
ATTTACAAAG TCACGGAAGG GCGACAGTCT GAAATATTCC ACCGCCATGC AGAAGGAAAC 
TTTGATCCCA GTTGCTGTTT TACCATATAT CATGGAAACC ACATGGAATC TCTGGACCTC 
ATAACATCAA ATCCGGAGGA GGCCCGAACT TGGATCACAG GGCTGAAATA TCTGATGGCT 
GGAATTAGCG ATGAGGATTC TCTGGCAAAA AGGCAAAGGA CCCATGATCA ATGGGTGAAG 
CAGACATTTG AAGAGGCTGA TAAAAATGGG GATGGCTTAC TCAACATTGA GGAAATATAC 
CAGTTAATGC ATAAACTCAA CGTGAATCTG CCACGAAGGA AAGTTCGCCA GATGTTCCAG 
GAAGCAGACA CGGACGAGAA CCAAGGGACT CTGACCTTTG AAGAGTTTTC TGTGTTTTAC 
AAGATGATGT CATTACGGAG GGATTTGTAC CTGTTACTTT TAAACTACAG CGACAAAAAG 
GATCACTTAA CAGTTGACGA TCTGGCTCAG TTTTTCAAAG TAGAACAAAA GATGAATAAT 
GTGACAACAG AATACTGTAT TGACATTATA CAGAAATTTG AAGTCTCAGA GGAAAACAAA 
GAACAACATG TTCTGGGAAT AGATGGTTTT ACTAATTTCA TGAGAAGTCC TGCAGGAGAT 
ATTTTTAACC CTCAGCACTG TGAGATCTAC CAAGACATGG ACCAACCTCT CTGTAGCTAC 
TACATTGCAT CATCCCATAA CACCTACTTA ACTGGAGACC AACTTCTTTC TCAATCCAAA 
GTGGATATGT ATGCTTGGGT ATTGCAAGAT GGCTGTCGAT GCATAGAAGT TGACTGCTGG 
GATGGACCAG ATGGGGAGCC AGTGGTACAC CATGGCTATA CCCTTACTTC CAAAGTCCTT 
TTTAAAGATG TTGTGGAAAC CATCAACAAA CATGCTTTTG TGAAAAATGA ATATCCTGTT 
ATACTTTCAA TTGAAAACCA CTGCAACATT CAACAACAAA GAAAGATTGC TCAGTACATG 
AAAGATATAT TTGGGGATAA ACTTGATCTG TCTTCTGTCC CACTTGGAGA ATCCAAGCAA 
CTTCCTAGTC CTCAGAATCT CAAGGGAAAG ATATTAGTGA AGGGGAAAAA GTTGCCATAT 
GACCTGGCAG ACGATGCTGA GGAGGGAGAA GTCTCAGATG AAGATAGTGC TGACGAAATA 
GAAGAAGAAT GCAAACTCAC ACAATGTTTT AGTAATGGCA CAACAGAGCA CCAGGTGGAA 
TCTTTCATTC GGAAAAAACT GGAGTCTTTG CTAAAGGAAT CCCAGATCAG GGATAAGGAA 
GATCCAGATA GCTTTACCGT GAAAGCATTA CTAAAGGCAA CACATGAAGG CCTGAATGTT 
AATCTTAAAC AGAATGATGA CACAAAAGAT TGTGGAAAGT CTAAAAGGTC TTCAGTTGGA 
AGTATTGCCA AACACAAAAA AGCTTCCAAA TCCAGGTCTA AATCTTGCAG TACTAGTGAT 
GATGAAGATT CATCGCCAAA AGAGACAACA GCACAGGCAC ACAGGTTGGG TCGTCGAAGG 
AAGACAATGA AGTTATGTCG AGCTTTGTCA GACTTGGTTG TCTACACAAA TTCTGTGGCT 
GCACAAGACA TTGTAGATGA TGGGACCTCA GGAAACGTTT TATCCTTTAG TGAAACAAGA 
GCACACCAGG TGGTTCAGCA CAAATCTGAG CAGCTCATGT TGTACAATCA AAAACAATTA 
ACTAGGATTT ATCCATCTGC ATACAGGATA GACTCTAGTA ATTTTAACCC AGTGGCCTAC 
TGGAATGCAG GAATTCAGCT TGTTGCGTTA AACTATCAAA CAGAAGGACG CATGATGGAA 
TTAAATCGAG CTAAATTCAA GATGAATGGT GGTTGTGGAT ATGTCCTTAA GCCCCAGCAG 
ATGTGCAAAG GTGTATTTAA CCAATTTTCA GCTGACCCTC TTCCTGCCAG CCCAAAAAAG 
CAGTTAATTT TGAAGATAAT CAGTGGACAG CAACTTCCCA AACCACCAGA TTCAATGCTT 
GGGGATAGAG GAGAGATCAT TGACCCTTTT GTAGAAGTGG AAATTATCGG CTTGCCTGTG 
GACTGCTGTA AGGAACAGAC TCGAGTAGTT GATGACAATG GTTTCAATCC TGTATGGGAG 
GAAACTCTTA CCTTTACAGT TCATATGCCA GAAATAGCAT TGGTAAGGTT CTTAGTATGG 
GACCATGATC CAATTGGCCG GGATTTTGTT GGACAAAGGA CTGTAGCCTT TAACAGTCTT 
ATGCCTGGAT ATCGGCATGT TTATCTGGAT GGTCTAACAG AAGCCTCCAT ATTTGTTCAT 
GTTACAATTA ATGAAATATA TGGAAAGAAC AGGCAGTTTA TTGGCCTGAA AGGGTTTTTC 
AATAGGAGTT CTAAGCATAG CTCTGTTGAG AGCAATAGTC ATGTTCGTAA GAAATCAATT 
GGGGACCGTA TCTTGCGACG CAGTGCGAGT GCTCCAGCCA AAGCCAGGAA GAAGACCAAG 
ATGGTGTTTC AAGAGTCAAC TGAGAGTAAA GACATTGTCT GTGAAACATC TGGTGCTAAA 
GAGAGAGATT TTATGAGAAA GAGAACCTCA AGGAGTCTGC AAGCACGCCC TGTTTCAATG 
CCTGTGGATA AGTTACTACT TGGGGCTTTT GAACCTTCAA CATCAGAGAT TTCTAAGGAG 
ACTAGAAGTG ACATAGCTTC AGGTTCAGCA GGGGAGATGC AAGCTAAGCA CCAGGAGAAG 
CCAGAGATGA CAAAACTGGA TCTACCAGTT CATAAAAATA ATCTTGCTAT TCAACATTCT 
TCCCCTCTTT TTAAAGATTG TCACCAGACT GAAAATCCAG TAAATAAACC TGTACATAAA 
AAATGGAGGA GCTTACATTT AGAAAAACAA AACTATGATG ATATTAGAGA GTTAAATGAT 
ACATTTACCA GTGAATCTGA TGAGGAGATT TTAAAGCCAA AAGAAGGTAA TGAAAAAGAA 
GCTATGCTGT TATTAGACAA AATGGAGTCC ATCCCAGAAA ACACCAAGCA TCATGCAAAA 
GCTGATTTAT TTTCTGTGAC TGATTTATCA AGCACTATAT GTACAGACAT GAATCACTTA 
CAGTCTACTG CACCTTTATT AGAAACTGAT ATTTCTCATA TTATAAGTGA AGTTCCTTCA 
GTCAACGAGA GTGAACTTGG AAGCACGGTG TCTGCTTTAA TAAGGCACTT TGAGATCACC 
GAAGACAAAA CTAATTTGAC ATTTGTCTCA AGTTTGCAAG GTCCAAACAG CCAAAGCAAT 
TTTTGTGACA ATTCTGATGT GTTAATATCT GACCTTTTAA ATAGAAAAAA GCACACAAGA 
CAATCTTTAC ATTCAGCTTC CAACACTACT GTGTTCTCCA GCCCTGAAAC AAGTGAATAT 
TCTATATATA CATTTATTCA TGAAGAGCAT GTACCTCCTG ATGCCAATTA CAAATTGATG 
GATGCAAACA ATGGCATATC ATCGTGTTCT AATTTAAAAA ATAATTCATC AAACAGTTGG 
CTAGATAGTT CTGAGAGCCT ACAGTTTTGT TCTCAATATA ATGCAAACAG CAATACATTA 
GGTCTAAAAT CAAAGCAAAG TAGGCCACAA GTTTCACTGA AAACATCATC AGGCTGTACA 
GATGAGAATG TCAAACAGAT ATCATCTCAC AACTCCTTTC TAAAGAACCT TAATGTAGAT 
AGTAATCCTA AGAGAATAGC GGATAAGAAT TTCTTACATA CTGGTGTCAA TAGGTATGCC 
ACACAAGCCT GTCCAAACTC TCAAAACACA GGCATTCAAC ACGGATCTGG TCAACTTTAT 
CAAAACAGGC ATTCAAGTTA TAAACCAAGA GCAGATGCAC CACGAGACAA ACAGTATATT 
GCCCAGTCTG GAAACCTGCA CAGCTCGCCC AATGCAAACT GGCCCAAACC TTCAAACTAT 
CACATTCATT CATTACAAGG TATTAAACAG CCAAGTCCAT GTATATCAAA AAGTCTTGGA 
GATCTGACAT CAGACGATAT ATCTTGTAAT TTTGAAAGTA AGTACAAACG GATCAACAGA 
GGGTTTCTTT CATCAGCTAT TTGTCCGGAA AATACATCAT TAGCTCAAAG CAGACCTCAG 
TCTACCGATG CTTTAACGGA ACAGTTGCGC AAACTGATGT CTTTTGACCA AGACGACAGT 
AACAGGCCAT CATATTATGA AGAAGAAGAA TGGGACAGTC CTAAGCCACT TACAAGAAAG 
CTATCCTCAA GGAGTCAAAG TAGGGTCAGA AACATTGCTA ACCGTGCCAA AGAAAGACAG 
GAGGCCAATA AAATAAAACA AAGCAATTTA AACTCAAGTG AAGTAGTCTT AAGAAACAAA 
CGTTCCATGG CTTCTCAGCC CATCAACAGG CATTCCACTG GTTCTTATAT AGGTAGCTTG 
TATAATAACT TTAACAGTGA AAGGCTAGAA GGCAGAGGTA TACCAGAAGG TGCATGCACT 
TCCCATACAC AAGTGTATAA TGACCACATT GACACTGGCT TTACTTATCG ACATGAGCCA 
AGCAATACTG ATGAACCAGA AATTTATTTC CTACTAAGAC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949871.1
CDS	281..5125
Translation	MSSQNQDKRRFLQYCRHFLADNGAFHVERCMSVMQSGTQMVKLKSGTKALVRLFYLDEHRTCIRWRPSRKSEKAKIIIDSIYKVTEGRQSEIFHRHAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIYQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFSVFYKMMSLRRDLYLLLLNYSDKKDHLTVDDLAQFFKVEQKMNNVTTEYCIDIIQKFEVSEENKEQHVLGIDGFTNFMRSPAGDIFNPQHCEIYQDMDQPLCSYYIASSHNTYLTGDQLLSQSKVDMYAWVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKVLFKDVVETINKHAFVKNEYPVILSIENHCNIQQQRKIAQYMKDIFGDKLDLSSVPLGESKQLPSPQNLKGKILVKGKKLPYDLADDAEEGEVSDEDSADEIEEECKLTQCFSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVKALLKATHEGLNVNLKQNDDTKDCGKSKRSSVGSIAKHKKASKSRSKSCSTSDDEDSSPKETTAQAHRLGRRRKTMKLCRALSDLVVYTNSVAAQDIVDDGTSGNVLSFSETRAHQVVQHKSEQLMLYNQKQLTRIYPSAYRIDSSNFNPVAYWNAGIQLVALNYQTEGRMMELNRAKFKMNGGCGYVLKPQQMCKGVFNQFSADPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKEQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVAFNSLMPGYRHVYLDGLTEASIFVHVTINEIYGKNRQFIGLKGFFNRSSKHSSVESNSHVRKKSIGDRILRRSASAPAKARKKTKMVFQESTESKDIVCETSGAKERDFMRKRTSRSLQARPVSMPVDKLLLGAFEPSTSEISKETRSDIASGSAGEMQAKHQEKPEMTKLDLPVHKNNLAIQHSSPLFKDCHQTENPVNKPVHKKWRSLHLEKQNYDDIRELNDTFTSESDEEILKPKEGNEKEAMLLLDKMESIPENTKHHAKADLFSVTDLSSTICTDMNHLQSTAPLLETDISHIISEVPSVNESELGSTVSALIRHFEITEDKTNLTFVSSLQGPNSQSNFCDNSDVLISDLLNRKKHTRQSLHSASNTTVFSSPETSEYSIYTFIHEEHVPPDANYKLMDANNGISSCSNLKNNSSNSWLDSSESLQFCSQYNANSNTLGLKSKQSRPQVSLKTSSGCTDENVKQISSHNSFLKNLNVDSNPKRIADKNFLHTGVNRYATQACPNSQNTGIQHGSGQLYQNRHSSYKPRADAPRDKQYIAQSGNLHSSPNANWPKPSNYHIHSLQGIKQPSPCISKSLGDLTSDDISCNFESKYKRINRGFLSSAICPENTSLAQSRPQSTDALTEQLRKLMSFDQDDSNRPSYYEEEEWDSPKPLTRKLSSRSQSRVRNIANRAKERQEANKIKQSNLNSSEVVLRNKRSMASQPINRHSTGSYIGSLYNNFNSERLEGRGIPEGACTSHTQVYNDHIDTGFTYRHEPSNTDEPEIYFLLRL

Target ORF information:

RefSeq Version	XM_018094382.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094382.1

ORF Insert Sequence:

ATGAGTTCCC AAAATCAGGA TAAGAGAAGA TTTCTGCAGT ACTGCCGCCA TTTCTTAGCT 
GACAATGGGG CATTTCATGT TGAAAGATGT ATGAGTGTAA TGCAGTCTGG GACACAGATG 
GTAAAACTAA AGAGTGGAAC AAAGGCTTTG GTTCGCCTCT TTTACCTGGA TGAACATCGG 
ACATGTATCA GATGGAGACC TTCAAGAAAA AGCGAGAAAG CTAAAATTAT AATTGATTCA 
ATTTACAAAG TCACGGAAGG GCGACAGTCT GAAATATTCC ACCGCCATGC AGAAGGAAAC 
TTTGATCCCA GTTGCTGTTT TACCATATAT CATGGAAACC ACATGGAATC TCTGGACCTC 
ATAACATCAA ATCCGGAGGA GGCCCGAACT TGGATCACAG GGCTGAAATA TCTGATGGCT 
GGAATTAGCG ATGAGGATTC TCTGGCAAAA AGGCAAAGGA CCCATGATCA ATGGGTGAAG 
CAGACATTTG AAGAGGCTGA TAAAAATGGG GATGGCTTAC TCAACATTGA GGAAATATAC 
CAGTTAATGC ATAAACTCAA CGTGAATCTG CCACGAAGGA AAGTTCGCCA GATGTTCCAG 
GAAGCAGACA CGGACGAGAA CCAAGGGACT CTGACCTTTG AAGAGTTTTC TGTGTTTTAC 
AAGATGATGT CATTACGGAG GGATTTGTAC CTGTTACTTT TAAACTACAG CGACAAAAAG 
GATCACTTAA CAGTTGACGA TCTGGCTCAG TTTTTCAAAG TAGAACAAAA GATGAATAAT 
GTGACAACAG AATACTGTAT TGACATTATA CAGAAATTTG AAGTCTCAGA GGAAAACAAA 
GAACAACATG TTCTGGGAAT AGATGGTTTT ACTAATTTCA TGAGAAGTCC TGCAGGAGAT 
ATTTTTAACC CTCAGCACTG TGAGATCTAC CAAGACATGG ACCAACCTCT CTGTAGCTAC 
TACATTGCAT CATCCCATAA CACCTACTTA ACTGGAGACC AACTTCTTTC TCAATCCAAA 
GTGGATATGT ATGCTTGGGT ATTGCAAGAT GGCTGTCGAT GCATAGAAGT TGACTGCTGG 
GATGGACCAG ATGGGGAGCC AGTGGTACAC CATGGCTATA CCCTTACTTC CAAAGTCCTT 
TTTAAAGATG TTGTGGAAAC CATCAACAAA CATGCTTTTG TGAAAAATGA ATATCCTGTT 
ATACTTTCAA TTGAAAACCA CTGCAACATT CAACAACAAA GAAAGATTGC TCAGTACATG 
AAAGATATAT TTGGGGATAA ACTTGATCTG TCTTCTGTCC CACTTGGAGA ATCCAAGCAA 
CTTCCTAGTC CTCAGAATCT CAAGGGAAAG ATATTAGTGA AGGGGAAAAA GTTGCCATAT 
GACCTGGCAG ACGATGCTGA GGAGGGAGAA GTCTCAGATG AAGATAGTGC TGACGAAATA 
GAAGAAGAAT GCAAACTCAC ACAATGTTTT AGTAATGGCA CAACAGAGCA CCAGGTGGAA 
TCTTTCATTC GGAAAAAACT GGAGTCTTTG CTAAAGGAAT CCCAGATCAG GGATAAGGAA 
GATCCAGATA GCTTTACCGT GAAAGCATTA CTAAAGGCAA CACATGAAGG CCTGAATGTT 
AATCTTAAAC AGAATGATGA CACAAAAGAT TGTGGAAAGT CTAAAAGGTC TTCAGTTGGA 
AGTATTGCCA AACACAAAAA AGCTTCCAAA TCCAGGTCTA AATCTTGCAG TACTAGTGAT 
GATGAAGATT CATCGCCAAA AGAGACAACA GCACAGGCAC ACAGGTTGGG TCGTCGAAGG 
AAGACAATGA AGTTATGTCG AGCTTTGTCA GACTTGGTTG TCTACACAAA TTCTGTGGCT 
GCACAAGACA TTGTAGATGA TGGGACCTCA GGAAACGTTT TATCCTTTAG TGAAACAAGA 
GCACACCAGG TGGTTCAGCA CAAATCTGAG CAGCTCATGT TGTACAATCA AAAACAATTA 
ACTAGGATTT ATCCATCTGC ATACAGGATA GACTCTAGTA ATTTTAACCC AGTGGCCTAC 
TGGAATGCAG GAATTCAGCT TGTTGCGTTA AACTATCAAA CAGAAGGACG CATGATGGAA 
TTAAATCGAG CTAAATTCAA GATGAATGGT GGTTGTGGAT ATGTCCTTAA GCCCCAGCAG 
ATGTGCAAAG GTGTATTTAA CCAATTTTCA GCTGACCCTC TTCCTGCCAG CCCAAAAAAG 
CAGTTAATTT TGAAGATAAT CAGTGGACAG CAACTTCCCA AACCACCAGA TTCAATGCTT 
GGGGATAGAG GAGAGATCAT TGACCCTTTT GTAGAAGTGG AAATTATCGG CTTGCCTGTG 
GACTGCTGTA AGGAACAGAC TCGAGTAGTT GATGACAATG GTTTCAATCC TGTATGGGAG 
GAAACTCTTA CCTTTACAGT TCATATGCCA GAAATAGCAT TGGTAAGGTT CTTAGTATGG 
GACCATGATC CAATTGGCCG GGATTTTGTT GGACAAAGGA CTGTAGCCTT TAACAGTCTT 
ATGCCTGGAT ATCGGCATGT TTATCTGGAT GGTCTAACAG AAGCCTCCAT ATTTGTTCAT 
GTTACAATTA ATGAAATATA TGGAAAGAAC AGGCAGTTTA TTGGCCTGAA AGGGTTTTTC 
AATAGGAGTT CTAAGCATAG CTCTGTTGAG AGCAATAGTC ATGTTCGTAA GAAATCAATT 
GGGGACCGTA TCTTGCGACG CAGTGCGAGT GCTCCAGCCA AAGCCAGGAA GAAGACCAAG 
ATGGTGTTTC AAGAGTCAAC TGAGAGTAAA GACATTGTCT GTGAAACATC TGGTGCTAAA 
GAGAGAGATT TTATGAGAAA GAGAACCTCA AGGAGTCTGC AAGCACGCCC TGTTTCAATG 
CCTGTGGATA AGTTACTACT TGGGGCTTTT GAACCTTCAA CATCAGAGAT TTCTAAGGAG 
ACTAGAAGTG ACATAGCTTC AGGTTCAGCA GGGGAGATGC AAGCTAAGCA CCAGGAGAAG 
CCAGAGATGA CAAAACTGGA TCTACCAGTT CATAAAAATA ATCTTGCTAT TCAACATTCT 
TCCCCTCTTT TTAAAGATTG TCACCAGACT GAAAATCCAG TAAATAAACC TGTACATAAA 
AAATGGAGGA GCTTACATTT AGAAAAACAA AACTATGATG ATATTAGAGA GTTAAATGAT 
ACATTTACCA GTGAATCTGA TGAGGAGATT TTAAAGCCAA AAGAAGGTAA TGAAAAAGAA 
GCTATGCTGT TATTAGACAA AATGGAGTCC ATCCCAGAAA ACACCAAGCA TCATGCAAAA 
GCTGATTTAT TTTCTGTGAC TGATTTATCA AGCACTATAT GTACAGACAT GAATCACTTA 
CAGTCTACTG CACCTTTATT AGAAACTGAT ATTTCTCATA TTATAAGTGA AGTTCCTTCA 
GTCAACGAGA GTGAACTTGG AAGCACGGTG TCTGCTTTAA TAAGGCACTT TGAGATCACC 
GAAGACAAAA CTAATTTGAC ATTTGTCTCA AGTTTGCAAG GTCCAAACAG CCAAAGCAAT 
TTTTGTGACA ATTCTGATGT GTTAATATCT GACCTTTTAA ATAGAAAAAA GCACACAAGA 
CAATCTTTAC ATTCAGCTTC CAACACTACT GTGTTCTCCA GCCCTGAAAC AAGTGAATAT 
TCTATATATA CATTTATTCA TGAAGAGCAT GTACCTCCTG ATGCCAATTA CAAATTGATG 
GATGCAAACA ATGGCATATC ATCGTGTTCT AATTTAAAAA ATAATTCATC AAACAGTTGG 
CTAGATAGTT CTGAGAGCCT ACAGTTTTGT TCTCAATATA ATGCAAACAG CAATACATTA 
GGTCTAAAAT CAAAGCAAAG TAGGCCACAA GTTTCACTGA AAACATCATC AGGCTGTACA 
GATGAGAATG TCAAACAGAT ATCATCTCAC AACTCCTTTC TAAAGAACCT TAATGTAGAT 
AGTAATCCTA AGAGAATAGC GGATAAGAAT TTCTTACATA CTGGTGTCAA TAGGTATGCC 
ACACAAGCCT GTCCAAACTC TCAAAACACA GGCATTCAAC ACGGATCTGG TCAACTTTAT 
CAAAACAGGC ATTCAAGTTA TAAACCAAGA GCAGATGCAC CACGAGACAA ACAGTATATT 
GCCCAGTCTG GAAACCTGCA CAGCTCGCCC AATGCAAACT GGCCCAAACC TTCAAACTAT 
CACATTCATT CATTACAAGG TATTAAACAG CCAAGTCCAT GTATATCAAA AAGTCTTGGA 
GATCTGACAT CAGACGATAT ATCTTGTAAT TTTGAAAGTA AGTACAAACG GATCAACAGA 
GGGTTTCTTT CATCAGCTAT TTGTCCGGAA AATACATCAT TAGCTCAAAG CAGACCTCAG 
TCTACCGATG CTTTAACGGA ACAGTTGCGC AAACTGATGT CTTTTGACCA AGACGACAGT 
AACAGGCCAT CATATTATGA AGAAGAAGAA TGGGACAGTC CTAAGCCACT TACAAGAAAG 
CTATCCTCAA GGAGTCAAAG TAGGGTCAGA AACATTGCTA ACCGTGCCAA AGAAAGACAG 
GAGGCCAATA AAATAAAACA AAGCAATTTA AACTCAAGTG AAGTAGTCTT AAGAAACAAA 
CGTTCCATGG CTTCTCAGCC CATCAACAGG CATTCCACTG GTTCTTATAT AGGTAGCTTG 
TATAATAACT TTAACAGTGA AAGGCTAGAA GGCAGAGGTA TACCAGAAGG TGCATGCACT 
TCCCATACAC AAGTGTATAA TGACCACATT GACACTGGCT TTACTTATCG ACATGAGCCA 
AGCAATACTG ATGAACCAGA AATTTATTTC CTACTAAGAC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plch1 ( XM_018094382.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949871.1 Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X5, mRNA.

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CloneID	OXa44744
Clone ID Related Accession (Same CDS sequence)	XM_018094382.1 , XM_018094382.2
Accession Version	XM_018094382.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018094382.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCC AAAATCAGGA TAAGAGAAGA TTTCTGCAGT ACTGCCGCCA TTTCTTAGCT 
GACAATGGGG CATTTCATGT TGAAAGATGT ATGAGTGTAA TGCAGTCTGG GACACAGATG 
GTAAAACTAA AGAGTGGAAC AAAGGCTTTG GTTCGCCTCT TTTACCTGGA TGAACATCGG 
ACATGTATCA GATGGAGACC TTCAAGAAAA AGCGAGAAAG CTAAAATTAT AATTGATTCA 
ATTTACAAAG TCACGGAAGG GCGACAGTCT GAAATATTCC ACCGCCATGC AGAAGGAAAC 
TTTGATCCCA GTTGCTGTTT TACCATATAT CATGGAAACC ACATGGAATC TCTGGACCTC 
ATAACATCAA ATCCGGAGGA GGCCCGAACT TGGATCACAG GGCTGAAATA TCTGATGGCT 
GGAATTAGCG ATGAGGATTC TCTGGCAAAA AGGCAAAGGA CCCATGATCA ATGGGTGAAG 
CAGACATTTG AAGAGGCTGA TAAAAATGGG GATGGCTTAC TCAACATTGA GGAAATATAC 
CAGTTAATGC ATAAACTCAA CGTGAATCTG CCACGAAGGA AAGTTCGCCA GATGTTCCAG 
GAAGCAGACA CGGACGAGAA CCAAGGGACT CTGACCTTTG AAGAGTTTTC TGTGTTTTAC 
AAGATGATGT CATTACGGAG GGATTTGTAC CTGTTACTTT TAAACTACAG CGACAAAAAG 
GATCACTTAA CAGTTGACGA TCTGGCTCAG TTTTTCAAAG TAGAACAAAA GATGAATAAT 
GTGACAACAG AATACTGTAT TGACATTATA CAGAAATTTG AAGTCTCAGA GGAAAACAAA 
GAACAACATG TTCTGGGAAT AGATGGTTTT ACTAATTTCA TGAGAAGTCC TGCAGGAGAT 
ATTTTTAACC CTCAGCACTG TGAGATCTAC CAAGACATGG ACCAACCTCT CTGTAGCTAC 
TACATTGCAT CATCCCATAA CACCTACTTA ACTGGAGACC AACTTCTTTC TCAATCCAAA 
GTGGATATGT ATGCTTGGGT ATTGCAAGAT GGCTGTCGAT GCATAGAAGT TGACTGCTGG 
GATGGACCAG ATGGGGAGCC AGTGGTACAC CATGGCTATA CCCTTACTTC CAAAGTCCTT 
TTTAAAGATG TTGTGGAAAC CATCAACAAA CATGCTTTTG TGAAAAATGA ATATCCTGTT 
ATACTTTCAA TTGAAAACCA CTGCAACATT CAACAACAAA GAAAGATTGC TCAGTACATG 
AAAGATATAT TTGGGGATAA ACTTGATCTG TCTTCTGTCC CACTTGGAGA ATCCAAGCAA 
CTTCCTAGTC CTCAGAATCT CAAGGGAAAG ATATTAGTGA AGGGGAAAAA GTTGCCATAT 
GACCTGGCAG ACGATGCTGA GGAGGGAGAA GTCTCAGATG AAGATAGTGC TGACGAAATA 
GAAGAAGAAT GCAAACTCAC ACAATGTTTT AGTAATGGCA CAACAGAGCA CCAGGTGGAA 
TCTTTCATTC GGAAAAAACT GGAGTCTTTG CTAAAGGAAT CCCAGATCAG GGATAAGGAA 
GATCCAGATA GCTTTACCGT GAAAGCATTA CTAAAGGCAA CACATGAAGG CCTGAATGTT 
AATCTTAAAC AGAATGATGA CACAAAAGAT TGTGGAAAGT CTAAAAGGTC TTCAGTTGGA 
AGTATTGCCA AACACAAAAA AGCTTCCAAA TCCAGGTCTA AATCTTGCAG TACTAGTGAT 
GATGAAGATT CATCGCCAAA AGAGACAACA GCACAGGCAC ACAGGTTGGG TCGTCGAAGG 
AAGACAATGA AGTTATGTCG AGCTTTGTCA GACTTGGTTG TCTACACAAA TTCTGTGGCT 
GCACAAGACA TTGTAGATGA TGGGACCTCA GGAAACGTTT TATCCTTTAG TGAAACAAGA 
GCACACCAGG TGGTTCAGCA CAAATCTGAG CAGCTCATGT TGTACAATCA AAAACAATTA 
ACTAGGATTT ATCCATCTGC ATACAGGATA GACTCTAGTA ATTTTAACCC AGTGGCCTAC 
TGGAATGCAG GAATTCAGCT TGTTGCGTTA AACTATCAAA CAGAAGGACG CATGATGGAA 
TTAAATCGAG CTAAATTCAA GATGAATGGT GGTTGTGGAT ATGTCCTTAA GCCCCAGCAG 
ATGTGCAAAG GTGTATTTAA CCAATTTTCA GCTGACCCTC TTCCTGCCAG CCCAAAAAAG 
CAGTTAATTT TGAAGATAAT CAGTGGACAG CAACTTCCCA AACCACCAGA TTCAATGCTT 
GGGGATAGAG GAGAGATCAT TGACCCTTTT GTAGAAGTGG AAATTATCGG CTTGCCTGTG 
GACTGCTGTA AGGAACAGAC TCGAGTAGTT GATGACAATG GTTTCAATCC TGTATGGGAG 
GAAACTCTTA CCTTTACAGT TCATATGCCA GAAATAGCAT TGGTAAGGTT CTTAGTATGG 
GACCATGATC CAATTGGCCG GGATTTTGTT GGACAAAGGA CTGTAGCCTT TAACAGTCTT 
ATGCCTGGAT ATCGGCATGT TTATCTGGAT GGTCTAACAG AAGCCTCCAT ATTTGTTCAT 
GTTACAATTA ATGAAATATA TGGAAAGAAC AGGCAGTTTA TTGGCCTGAA AGGGTTTTTC 
AATAGGAGTT CTAAGCATAG CTCTGTTGAG AGCAATAGTC ATGTTCGTAA GAAATCAATT 
GGGGACCGTA TCTTGCGACG CAGTGCGAGT GCTCCAGCCA AAGCCAGGAA GAAGACCAAG 
ATGGTGTTTC AAGAGTCAAC TGAGAGTAAA GACATTGTCT GTGAAACATC TGGTGCTAAA 
GAGAGAGATT TTATGAGAAA GAGAACCTCA AGGAGTCTGC AAGCACGCCC TGTTTCAATG 
CCTGTGGATA AGTTACTACT TGGGGCTTTT GAACCTTCAA CATCAGAGAT TTCTAAGGAG 
ACTAGAAGTG ACATAGCTTC AGGTTCAGCA GGGGAGATGC AAGCTAAGCA CCAGGAGAAG 
CCAGAGATGA CAAAACTGGA TCTACCAGTT CATAAAAATA ATCTTGCTAT TCAACATTCT 
TCCCCTCTTT TTAAAGATTG TCACCAGACT GAAAATCCAG TAAATAAACC TGTACATAAA 
AAATGGAGGA GCTTACATTT AGAAAAACAA AACTATGATG ATATTAGAGA GTTAAATGAT 
ACATTTACCA GTGAATCTGA TGAGGAGATT TTAAAGCCAA AAGAAGGTAA TGAAAAAGAA 
GCTATGCTGT TATTAGACAA AATGGAGTCC ATCCCAGAAA ACACCAAGCA TCATGCAAAA 
GCTGATTTAT TTTCTGTGAC TGATTTATCA AGCACTATAT GTACAGACAT GAATCACTTA 
CAGTCTACTG CACCTTTATT AGAAACTGAT ATTTCTCATA TTATAAGTGA AGTTCCTTCA 
GTCAACGAGA GTGAACTTGG AAGCACGGTG TCTGCTTTAA TAAGGCACTT TGAGATCACC 
GAAGACAAAA CTAATTTGAC ATTTGTCTCA AGTTTGCAAG GTCCAAACAG CCAAAGCAAT 
TTTTGTGACA ATTCTGATGT GTTAATATCT GACCTTTTAA ATAGAAAAAA GCACACAAGA 
CAATCTTTAC ATTCAGCTTC CAACACTACT GTGTTCTCCA GCCCTGAAAC AAGTGAATAT 
TCTATATATA CATTTATTCA TGAAGAGCAT GTACCTCCTG ATGCCAATTA CAAATTGATG 
GATGCAAACA ATGGCATATC ATCGTGTTCT AATTTAAAAA ATAATTCATC AAACAGTTGG 
CTAGATAGTT CTGAGAGCCT ACAGTTTTGT TCTCAATATA ATGCAAACAG CAATACATTA 
GGTCTAAAAT CAAAGCAAAG TAGGCCACAA GTTTCACTGA AAACATCATC AGGCTGTACA 
GATGAGAATG TCAAACAGAT ATCATCTCAC AACTCCTTTC TAAAGAACCT TAATGTAGAT 
AGTAATCCTA AGAGAATAGC GGATAAGAAT TTCTTACATA CTGGTGTCAA TAGGTATGCC 
ACACAAGCCT GTCCAAACTC TCAAAACACA GGCATTCAAC ACGGATCTGG TCAACTTTAT 
CAAAACAGGC ATTCAAGTTA TAAACCAAGA GCAGATGCAC CACGAGACAA ACAGTATATT 
GCCCAGTCTG GAAACCTGCA CAGCTCGCCC AATGCAAACT GGCCCAAACC TTCAAACTAT 
CACATTCATT CATTACAAGG TATTAAACAG CCAAGTCCAT GTATATCAAA AAGTCTTGGA 
GATCTGACAT CAGACGATAT ATCTTGTAAT TTTGAAAGTA AGTACAAACG GATCAACAGA 
GGGTTTCTTT CATCAGCTAT TTGTCCGGAA AATACATCAT TAGCTCAAAG CAGACCTCAG 
TCTACCGATG CTTTAACGGA ACAGTTGCGC AAACTGATGT CTTTTGACCA AGACGACAGT 
AACAGGCCAT CATATTATGA AGAAGAAGAA TGGGACAGTC CTAAGCCACT TACAAGAAAG 
CTATCCTCAA GGAGTCAAAG TAGGGTCAGA AACATTGCTA ACCGTGCCAA AGAAAGACAG 
GAGGCCAATA AAATAAAACA AAGCAATTTA AACTCAAGTG AAGTAGTCTT AAGAAACAAA 
CGTTCCATGG CTTCTCAGCC CATCAACAGG CATTCCACTG GTTCTTATAT AGGTAGCTTG 
TATAATAACT TTAACAGTGA AAGGCTAGAA GGCAGAGGTA TACCAGAAGG TGCATGCACT 
TCCCATACAC AAGTGTATAA TGACCACATT GACACTGGCT TTACTTATCG ACATGAGCCA 
AGCAATACTG ATGAACCAGA AATTTATTTC CTACTAAGAC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949871.1
CDS	248..5092
Translation	MSSQNQDKRRFLQYCRHFLADNGAFHVERCMSVMQSGTQMVKLKSGTKALVRLFYLDEHRTCIRWRPSRKSEKAKIIIDSIYKVTEGRQSEIFHRHAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIYQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFSVFYKMMSLRRDLYLLLLNYSDKKDHLTVDDLAQFFKVEQKMNNVTTEYCIDIIQKFEVSEENKEQHVLGIDGFTNFMRSPAGDIFNPQHCEIYQDMDQPLCSYYIASSHNTYLTGDQLLSQSKVDMYAWVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKVLFKDVVETINKHAFVKNEYPVILSIENHCNIQQQRKIAQYMKDIFGDKLDLSSVPLGESKQLPSPQNLKGKILVKGKKLPYDLADDAEEGEVSDEDSADEIEEECKLTQCFSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVKALLKATHEGLNVNLKQNDDTKDCGKSKRSSVGSIAKHKKASKSRSKSCSTSDDEDSSPKETTAQAHRLGRRRKTMKLCRALSDLVVYTNSVAAQDIVDDGTSGNVLSFSETRAHQVVQHKSEQLMLYNQKQLTRIYPSAYRIDSSNFNPVAYWNAGIQLVALNYQTEGRMMELNRAKFKMNGGCGYVLKPQQMCKGVFNQFSADPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKEQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVAFNSLMPGYRHVYLDGLTEASIFVHVTINEIYGKNRQFIGLKGFFNRSSKHSSVESNSHVRKKSIGDRILRRSASAPAKARKKTKMVFQESTESKDIVCETSGAKERDFMRKRTSRSLQARPVSMPVDKLLLGAFEPSTSEISKETRSDIASGSAGEMQAKHQEKPEMTKLDLPVHKNNLAIQHSSPLFKDCHQTENPVNKPVHKKWRSLHLEKQNYDDIRELNDTFTSESDEEILKPKEGNEKEAMLLLDKMESIPENTKHHAKADLFSVTDLSSTICTDMNHLQSTAPLLETDISHIISEVPSVNESELGSTVSALIRHFEITEDKTNLTFVSSLQGPNSQSNFCDNSDVLISDLLNRKKHTRQSLHSASNTTVFSSPETSEYSIYTFIHEEHVPPDANYKLMDANNGISSCSNLKNNSSNSWLDSSESLQFCSQYNANSNTLGLKSKQSRPQVSLKTSSGCTDENVKQISSHNSFLKNLNVDSNPKRIADKNFLHTGVNRYATQACPNSQNTGIQHGSGQLYQNRHSSYKPRADAPRDKQYIAQSGNLHSSPNANWPKPSNYHIHSLQGIKQPSPCISKSLGDLTSDDISCNFESKYKRINRGFLSSAICPENTSLAQSRPQSTDALTEQLRKLMSFDQDDSNRPSYYEEEEWDSPKPLTRKLSSRSQSRVRNIANRAKERQEANKIKQSNLNSSEVVLRNKRSMASQPINRHSTGSYIGSLYNNFNSERLEGRGIPEGACTSHTQVYNDHIDTGFTYRHEPSNTDEPEIYFLLRL

Target ORF information:

RefSeq Version	XM_018094382.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094382.2

ORF Insert Sequence:

ATGAGTTCCC AAAATCAGGA TAAGAGAAGA TTTCTGCAGT ACTGCCGCCA TTTCTTAGCT 
GACAATGGGG CATTTCATGT TGAAAGATGT ATGAGTGTAA TGCAGTCTGG GACACAGATG 
GTAAAACTAA AGAGTGGAAC AAAGGCTTTG GTTCGCCTCT TTTACCTGGA TGAACATCGG 
ACATGTATCA GATGGAGACC TTCAAGAAAA AGCGAGAAAG CTAAAATTAT AATTGATTCA 
ATTTACAAAG TCACGGAAGG GCGACAGTCT GAAATATTCC ACCGCCATGC AGAAGGAAAC 
TTTGATCCCA GTTGCTGTTT TACCATATAT CATGGAAACC ACATGGAATC TCTGGACCTC 
ATAACATCAA ATCCGGAGGA GGCCCGAACT TGGATCACAG GGCTGAAATA TCTGATGGCT 
GGAATTAGCG ATGAGGATTC TCTGGCAAAA AGGCAAAGGA CCCATGATCA ATGGGTGAAG 
CAGACATTTG AAGAGGCTGA TAAAAATGGG GATGGCTTAC TCAACATTGA GGAAATATAC 
CAGTTAATGC ATAAACTCAA CGTGAATCTG CCACGAAGGA AAGTTCGCCA GATGTTCCAG 
GAAGCAGACA CGGACGAGAA CCAAGGGACT CTGACCTTTG AAGAGTTTTC TGTGTTTTAC 
AAGATGATGT CATTACGGAG GGATTTGTAC CTGTTACTTT TAAACTACAG CGACAAAAAG 
GATCACTTAA CAGTTGACGA TCTGGCTCAG TTTTTCAAAG TAGAACAAAA GATGAATAAT 
GTGACAACAG AATACTGTAT TGACATTATA CAGAAATTTG AAGTCTCAGA GGAAAACAAA 
GAACAACATG TTCTGGGAAT AGATGGTTTT ACTAATTTCA TGAGAAGTCC TGCAGGAGAT 
ATTTTTAACC CTCAGCACTG TGAGATCTAC CAAGACATGG ACCAACCTCT CTGTAGCTAC 
TACATTGCAT CATCCCATAA CACCTACTTA ACTGGAGACC AACTTCTTTC TCAATCCAAA 
GTGGATATGT ATGCTTGGGT ATTGCAAGAT GGCTGTCGAT GCATAGAAGT TGACTGCTGG 
GATGGACCAG ATGGGGAGCC AGTGGTACAC CATGGCTATA CCCTTACTTC CAAAGTCCTT 
TTTAAAGATG TTGTGGAAAC CATCAACAAA CATGCTTTTG TGAAAAATGA ATATCCTGTT 
ATACTTTCAA TTGAAAACCA CTGCAACATT CAACAACAAA GAAAGATTGC TCAGTACATG 
AAAGATATAT TTGGGGATAA ACTTGATCTG TCTTCTGTCC CACTTGGAGA ATCCAAGCAA 
CTTCCTAGTC CTCAGAATCT CAAGGGAAAG ATATTAGTGA AGGGGAAAAA GTTGCCATAT 
GACCTGGCAG ACGATGCTGA GGAGGGAGAA GTCTCAGATG AAGATAGTGC TGACGAAATA 
GAAGAAGAAT GCAAACTCAC ACAATGTTTT AGTAATGGCA CAACAGAGCA CCAGGTGGAA 
TCTTTCATTC GGAAAAAACT GGAGTCTTTG CTAAAGGAAT CCCAGATCAG GGATAAGGAA 
GATCCAGATA GCTTTACCGT GAAAGCATTA CTAAAGGCAA CACATGAAGG CCTGAATGTT 
AATCTTAAAC AGAATGATGA CACAAAAGAT TGTGGAAAGT CTAAAAGGTC TTCAGTTGGA 
AGTATTGCCA AACACAAAAA AGCTTCCAAA TCCAGGTCTA AATCTTGCAG TACTAGTGAT 
GATGAAGATT CATCGCCAAA AGAGACAACA GCACAGGCAC ACAGGTTGGG TCGTCGAAGG 
AAGACAATGA AGTTATGTCG AGCTTTGTCA GACTTGGTTG TCTACACAAA TTCTGTGGCT 
GCACAAGACA TTGTAGATGA TGGGACCTCA GGAAACGTTT TATCCTTTAG TGAAACAAGA 
GCACACCAGG TGGTTCAGCA CAAATCTGAG CAGCTCATGT TGTACAATCA AAAACAATTA 
ACTAGGATTT ATCCATCTGC ATACAGGATA GACTCTAGTA ATTTTAACCC AGTGGCCTAC 
TGGAATGCAG GAATTCAGCT TGTTGCGTTA AACTATCAAA CAGAAGGACG CATGATGGAA 
TTAAATCGAG CTAAATTCAA GATGAATGGT GGTTGTGGAT ATGTCCTTAA GCCCCAGCAG 
ATGTGCAAAG GTGTATTTAA CCAATTTTCA GCTGACCCTC TTCCTGCCAG CCCAAAAAAG 
CAGTTAATTT TGAAGATAAT CAGTGGACAG CAACTTCCCA AACCACCAGA TTCAATGCTT 
GGGGATAGAG GAGAGATCAT TGACCCTTTT GTAGAAGTGG AAATTATCGG CTTGCCTGTG 
GACTGCTGTA AGGAACAGAC TCGAGTAGTT GATGACAATG GTTTCAATCC TGTATGGGAG 
GAAACTCTTA CCTTTACAGT TCATATGCCA GAAATAGCAT TGGTAAGGTT CTTAGTATGG 
GACCATGATC CAATTGGCCG GGATTTTGTT GGACAAAGGA CTGTAGCCTT TAACAGTCTT 
ATGCCTGGAT ATCGGCATGT TTATCTGGAT GGTCTAACAG AAGCCTCCAT ATTTGTTCAT 
GTTACAATTA ATGAAATATA TGGAAAGAAC AGGCAGTTTA TTGGCCTGAA AGGGTTTTTC 
AATAGGAGTT CTAAGCATAG CTCTGTTGAG AGCAATAGTC ATGTTCGTAA GAAATCAATT 
GGGGACCGTA TCTTGCGACG CAGTGCGAGT GCTCCAGCCA AAGCCAGGAA GAAGACCAAG 
ATGGTGTTTC AAGAGTCAAC TGAGAGTAAA GACATTGTCT GTGAAACATC TGGTGCTAAA 
GAGAGAGATT TTATGAGAAA GAGAACCTCA AGGAGTCTGC AAGCACGCCC TGTTTCAATG 
CCTGTGGATA AGTTACTACT TGGGGCTTTT GAACCTTCAA CATCAGAGAT TTCTAAGGAG 
ACTAGAAGTG ACATAGCTTC AGGTTCAGCA GGGGAGATGC AAGCTAAGCA CCAGGAGAAG 
CCAGAGATGA CAAAACTGGA TCTACCAGTT CATAAAAATA ATCTTGCTAT TCAACATTCT 
TCCCCTCTTT TTAAAGATTG TCACCAGACT GAAAATCCAG TAAATAAACC TGTACATAAA 
AAATGGAGGA GCTTACATTT AGAAAAACAA AACTATGATG ATATTAGAGA GTTAAATGAT 
ACATTTACCA GTGAATCTGA TGAGGAGATT TTAAAGCCAA AAGAAGGTAA TGAAAAAGAA 
GCTATGCTGT TATTAGACAA AATGGAGTCC ATCCCAGAAA ACACCAAGCA TCATGCAAAA 
GCTGATTTAT TTTCTGTGAC TGATTTATCA AGCACTATAT GTACAGACAT GAATCACTTA 
CAGTCTACTG CACCTTTATT AGAAACTGAT ATTTCTCATA TTATAAGTGA AGTTCCTTCA 
GTCAACGAGA GTGAACTTGG AAGCACGGTG TCTGCTTTAA TAAGGCACTT TGAGATCACC 
GAAGACAAAA CTAATTTGAC ATTTGTCTCA AGTTTGCAAG GTCCAAACAG CCAAAGCAAT 
TTTTGTGACA ATTCTGATGT GTTAATATCT GACCTTTTAA ATAGAAAAAA GCACACAAGA 
CAATCTTTAC ATTCAGCTTC CAACACTACT GTGTTCTCCA GCCCTGAAAC AAGTGAATAT 
TCTATATATA CATTTATTCA TGAAGAGCAT GTACCTCCTG ATGCCAATTA CAAATTGATG 
GATGCAAACA ATGGCATATC ATCGTGTTCT AATTTAAAAA ATAATTCATC AAACAGTTGG 
CTAGATAGTT CTGAGAGCCT ACAGTTTTGT TCTCAATATA ATGCAAACAG CAATACATTA 
GGTCTAAAAT CAAAGCAAAG TAGGCCACAA GTTTCACTGA AAACATCATC AGGCTGTACA 
GATGAGAATG TCAAACAGAT ATCATCTCAC AACTCCTTTC TAAAGAACCT TAATGTAGAT 
AGTAATCCTA AGAGAATAGC GGATAAGAAT TTCTTACATA CTGGTGTCAA TAGGTATGCC 
ACACAAGCCT GTCCAAACTC TCAAAACACA GGCATTCAAC ACGGATCTGG TCAACTTTAT 
CAAAACAGGC ATTCAAGTTA TAAACCAAGA GCAGATGCAC CACGAGACAA ACAGTATATT 
GCCCAGTCTG GAAACCTGCA CAGCTCGCCC AATGCAAACT GGCCCAAACC TTCAAACTAT 
CACATTCATT CATTACAAGG TATTAAACAG CCAAGTCCAT GTATATCAAA AAGTCTTGGA 
GATCTGACAT CAGACGATAT ATCTTGTAAT TTTGAAAGTA AGTACAAACG GATCAACAGA 
GGGTTTCTTT CATCAGCTAT TTGTCCGGAA AATACATCAT TAGCTCAAAG CAGACCTCAG 
TCTACCGATG CTTTAACGGA ACAGTTGCGC AAACTGATGT CTTTTGACCA AGACGACAGT 
AACAGGCCAT CATATTATGA AGAAGAAGAA TGGGACAGTC CTAAGCCACT TACAAGAAAG 
CTATCCTCAA GGAGTCAAAG TAGGGTCAGA AACATTGCTA ACCGTGCCAA AGAAAGACAG 
GAGGCCAATA AAATAAAACA AAGCAATTTA AACTCAAGTG AAGTAGTCTT AAGAAACAAA 
CGTTCCATGG CTTCTCAGCC CATCAACAGG CATTCCACTG GTTCTTATAT AGGTAGCTTG 
TATAATAACT TTAACAGTGA AAGGCTAGAA GGCAGAGGTA TACCAGAAGG TGCATGCACT 
TCCCATACAC AAGTGTATAA TGACCACATT GACACTGGCT TTACTTATCG ACATGAGCCA 
AGCAATACTG ATGAACCAGA AATTTATTTC CTACTAAGAC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plch1 ( XM_031902336.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758196.1 Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X1, mRNA.

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CloneID	OXa58658
Clone ID Related Accession (Same CDS sequence)	XM_031902336.1
Accession Version	XM_031902336.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCC AAAATCAGGA TAAGAGAAGA TTTCTGCAGT ACTGCCGCCA TTTCTTAGCT 
GACAATGGGG CATTTCATGT TGAAAGATGT ATGAGTGTAA TGCAGTCTGG GACACAGATG 
GTAAAACTAA AGAGTGGAAC AAAGGCTTTG GTTCGCCTCT TTTACCTGGA TGAACATCGG 
ACATGTATCA GATGGAGACC TTCAAGAAAA AGCGAGAAAG CTAAAATTAT AATTGATTCA 
ATTTACAAAG TCACGGAAGG GCGACAGTCT GAAATATTCC ACCGCCATGC AGAAGGAAAC 
TTTGATCCCA GTTGCTGTTT TACCATATAT CATGGAAACC ACATGGAATC TCTGGACCTC 
ATAACATCAA ATCCGGAGGA GGCCCGAACT TGGATCACAG GGCTGAAATA TCTGATGGCT 
GGAATTAGCG ATGAGGATTC TCTGGCAAAA AGGCAAAGGA CCCATGATCA ATGGGTGAAG 
CAGACATTTG AAGAGGCTGA TAAAAATGGG GATGGCTTAC TCAACATTGA GGAAATATAC 
CAGTTAATGC ATAAACTCAA CGTGAATCTG CCACGAAGGA AAGTTCGCCA GATGTTCCAG 
GAAGCAGACA CGGACGAGAA CCAAGGGACT CTGACCTTTG AAGAGTTTTC TGTGTTTTAC 
AAGATGATGT CATTACGGAG GGATTTGTAC CTGTTACTTT TAAACTACAG CGACAAAAAG 
GATCACTTAA CAGTTGACGA TCTGGCTCAG TTTTTCAAAG TAGAACAAAA GATGAATAAT 
GTGACAACAG AATACTGTAT TGACATTATA CAGAAATTTG AAGTCTCAGA GGAAAACAAA 
GAACAACATG TTCTGGGAAT AGATGGTTTT ACTAATTTCA TGAGAAGTCC TGCAGGAGAT 
ATTTTTAACC CTCAGCACTG TGAGATCTAC CAAGACATGG ACCAACCTCT CTGTAGCTAC 
TACATTGCAT CATCCCATAA CACCTACTTA ACTGGAGACC AACTTCTTTC TCAATCCAAA 
GTGGATATGT ATGCTTGGGT ATTGCAAGAT GGCTGTCGAT GCATAGAAGT TGACTGCTGG 
GATGGACCAG ATGGGGAGCC AGTGGTACAC CATGGCTATA CCCTTACTTC CAAAGTCCTT 
TTTAAAGATG TTGTGGAAAC CATCAACAAA CATGCTTTTG TGAAAAATGA ATATCCTGTT 
ATACTTTCAA TTGAAAACCA CTGCAACATT CAACAACAAA GAAAGATTGC TCAGTACATG 
AAAGATATAT TTGGGGATAA ACTTGATCTG TCTTCTGTCC CACTTGGAGA ATCCAAGCAA 
CTTCCTAGTC CTCAGAATCT CAAGGGAAAG ATATTAGTGA AGGGGAAAAA GTTGCCATAT 
GACCTGGCAG ACGATGCTGA GGAGGGAGAA GTCTCAGATG AAGATAGTGC TGACGAAATA 
GAAGAAGAAT GCAAACTCAC ACAATGTTTT AGTAATGGCA CAACAGAGCA CCAGGTGGAA 
TCTTTCATTC GGAAAAAACT GGAGTCTTTG CTAAAGGAAT CCCAGATCAG GGATAAGGAA 
GATCCAGATA GCTTTACCGT GAAAGCATTA CTAAAGGCAA CACATGAAGG CCTGAATGTT 
AATCTTAAAC AGAATGATGA CACAAAAGAT TGTGGAAAGT CTAAAAGGTC TTCAGTTGGA 
AGTATTGCCA AACACAAAAA AGCTTCCAAA TCCAGGTCTA AATCTTGCAG TACTAGTGAT 
GATGAAGATT CATCGCCAAA AGAGACAACA GCACAGGCAC ACAGGTGGAG CCAAGCTACA 
TATGGCATCT TGCTTACTTG TGCTTGGATT GGTGGAGAGG AAGATGTTGA ATATGCAAAT 
TACAGGTTGG GTCGTCGAAG GAAGACAATG AAGTTATGTC GAGCTTTGTC AGACTTGGTT 
GTCTACACAA ATTCTGTGGC TGCACAAGAC ATTGTAGATG ATGGGACCTC AGGAAACGTT 
TTATCCTTTA GTGAAACAAG AGCACACCAG GTGGTTCAGC ACAAATCTGA GCAGCTCATG 
TTGTACAATC AAAAACAATT AACTAGGATT TATCCATCTG CATACAGGAT AGACTCTAGT 
AATTTTAACC CAGTGGCCTA CTGGAATGCA GGAATTCAGC TTGTTGCGTT AAACTATCAA 
ACAGAAGGAC GCATGATGGA ATTAAATCGA GCTAAATTCA AGATGAATGG TGGTTGTGGA 
TATGTCCTTA AGCCCCAGCA GATGTGCAAA GGTGTATTTA ACCAATTTTC AGCTGACCCT 
CTTCCTGCCA GCCCAAAAAA GCAGTTAATT TTGAAGATAA TCAGTGGACA GCAACTTCCC 
AAACCACCAG ATTCAATGCT TGGGGATAGA GGAGAGATCA TTGACCCTTT TGTAGAAGTG 
GAAATTATCG GCTTGCCTGT GGACTGCTGT AAGGAACAGA CTCGAGTAGT TGATGACAAT 
GGTTTCAATC CTGTATGGGA GGAAACTCTT ACCTTTACAG TTCATATGCC AGAAATAGCA 
TTGGTAAGGT TCTTAGTATG GGACCATGAT CCAATTGGCC GGGATTTTGT TGGACAAAGG 
ACTGTAGCCT TTAACAGTCT TATGCCTGGA TATCGGCATG TTTATCTGGA TGGTCTAACA 
GAAGCCTCCA TATTTGTTCA TGTTACAATT AATGAAATAT ATGGAAAGTG GAGTCCTTTA 
ATCCTCAATC CCAGTTACAC AATATTGCAC TTTCTAGGAG CTACGAAGAA CAGGCAGTTT 
ATTGGCCTGA AAGGGTTTTT CAATAGGAGT TCTAAGCATA GCTCTGTTGA GAGCAATAGT 
CATGTTCGTA AGAAATCAAT TGGGGACCGT ATCTTGCGAC GCAGTGCGAG TGCTCCAGCC 
AAAGCCAGGA AGAAGACCAA GATGGTGTTT CAAGAGTCAA CTGAGAGTAA AGACATTGTC 
TGTGAAACAT CTGGTGCTAA AGAGAGAGAT TTTATGAGAA AGAGAACCTC AAGGAGTCTG 
CAAGCACGCC CTGTTTCAAT GCCTGTGGAT AAGTTACTAC TTGGGGCTTT TGAACCTTCA 
ACATCAGAGA TTTCTAAGGA GACTAGAAGT GACATAGCTT CAGGTTCAGC AGGGGAGATG 
CAAGCTAAGC ACCAGGAGAA GCCAGAGATG ACAAAACTGG ATCTACCAGT TCATAAAAAT 
AATCTTGCTA TTCAACATTC TTCCCCTCTT TTTAAAGATT GTCACCAGAC TGAAAATCCA 
GTAAATAAAC CTGTACATAA AAAATGGAGG AGCTTACATT TAGAAAAACA AAACTATGAT 
GATATTAGAG AGTTAAATGA TACATTTACC AGTGAATCTG ATGAGGAGAT TTTAAAGCCA 
AAAGAAGGTA ATGAAAAAGA AGCTATGCTG TTATTAGACA AAATGGAGTC CATCCCAGAA 
AACACCAAGC ATCATGCAAA AGCTGATTTA TTTTCTGTGA CTGATTTATC AAGCACTATA 
TGTACAGACA TGAATCACTT ACAGTCTACT GCACCTTTAT TAGAAACTGA TATTTCTCAT 
ATTATAAGTG AAGTTCCTTC AGTCAACGAG AGTGAACTTG GAAGCACGGT GTCTGCTTTA 
ATAAGGCACT TTGAGATCAC CGAAGACAAA ACTAATTTGA CATTTGTCTC AAGTTTGCAA 
GGTCCAAACA GCCAAAGCAA TTTTTGTGAC AATTCTGATG TGTTAATATC TGACCTTTTA 
AATAGAAAAA AGCACACAAG ACAATCTTTA CATTCAGCTT CCAACACTAC TGTGTTCTCC 
AGCCCTGAAA CAAGTGAATA TTCTATATAT ACATTTATTC ATGAAGAGCA TGTACCTCCT 
GATGCCAATT ACAAATTGAT GGATGCAAAC AATGGCATAT CATCGTGTTC TAATTTAAAA 
AATAATTCAT CAAACAGTTG GCTAGATAGT TCTGAGAGCC TACAGTTTTG TTCTCAATAT 
AATGCAAACA GCAATACATT AGGTCTAAAA TCAAAGCAAA GTAGGCCACA AGTTTCACTG 
AAAACATCAT CAGGCTGTAC AGATGAGAAT GTCAAACAGA TATCATCTCA CAACTCCTTT 
CTAAAGAACC TTAATGTAGA TAGTAATCCT AAGAGAATAG CGGATAAGAA TTTCTTACAT 
ACTGGTGTCA ATAGGTATGC CACACAAGCC TGTCCAAACT CTCAAAACAC AGGCATTCAA 
CACGGATCTG GTCAACTTTA TCAAAACAGG CATTCAAGTT ATAAACCAAG AGCAGATGCA 
CCACGAGACA AACAGTATAT TGCCCAGTCT GGAAACCTGC ACAGCTCGCC CAATGCAAAC 
TGGCCCAAAC CTTCAAACTA TCACATTCAT TCATTACAAG GTATTAAACA GCCAAGTCCA 
TGTATATCAA AAAGTCTTGG AGATCTGACA TCAGACGATA TATCTTGTAA TTTTGAAAGT 
AAGTACAAAC GGATCAACAG AGGGTTTCTT TCATCAGCTA TTTGTCCGGA AAATACATCA 
TTAGCTCAAA GCAGACCTCA GTCTACCGAT GCTTTAACGG AACAGTTGCG CAAACTGATG 
TCTTTTGACC AAGACGACAG TAACAGGCCA TCATATTATG AAGAAGAAGA ATGGGACAGT 
CCTAAGCCAC TTACAAGAAA GCTATCCTCA AGGAGTCAAA GTAGGGTCAG AAACATTGCT 
AACCGTGCCA AAGAAAGACA GGAGGCCAAT AAAATAAAAC AAAGCAATTT AAACTCAAGT 
GAAGTAGTCT TAAGAAACAA ACGTTCCATG GCTTCTCAGC CCATCAACAG GCATTCCACT 
GGTTCTTATA TAGGTAGCTT GTATAATAAC TTTAACAGTG AAAGGCTAGA AGGCAGAGGT 
ATACCAGAAG GTGCATGCAC TTCCCATACA CAAGTGTATA ATGACCACAT TGACACTGGC 
TTTACTTATC GACATGAGCC AAGCAATACT GATGAACCAG AAATTTATTT CCTACTAAGA 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758196.1
CDS	250..5235
Translation	MSSQNQDKRRFLQYCRHFLADNGAFHVERCMSVMQSGTQMVKLKSGTKALVRLFYLDEHRTCIRWRPSRKSEKAKIIIDSIYKVTEGRQSEIFHRHAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIYQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFSVFYKMMSLRRDLYLLLLNYSDKKDHLTVDDLAQFFKVEQKMNNVTTEYCIDIIQKFEVSEENKEQHVLGIDGFTNFMRSPAGDIFNPQHCEIYQDMDQPLCSYYIASSHNTYLTGDQLLSQSKVDMYAWVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKVLFKDVVETINKHAFVKNEYPVILSIENHCNIQQQRKIAQYMKDIFGDKLDLSSVPLGESKQLPSPQNLKGKILVKGKKLPYDLADDAEEGEVSDEDSADEIEEECKLTQCFSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVKALLKATHEGLNVNLKQNDDTKDCGKSKRSSVGSIAKHKKASKSRSKSCSTSDDEDSSPKETTAQAHRWSQATYGILLTCAWIGGEEDVEYANYRLGRRRKTMKLCRALSDLVVYTNSVAAQDIVDDGTSGNVLSFSETRAHQVVQHKSEQLMLYNQKQLTRIYPSAYRIDSSNFNPVAYWNAGIQLVALNYQTEGRMMELNRAKFKMNGGCGYVLKPQQMCKGVFNQFSADPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKEQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVAFNSLMPGYRHVYLDGLTEASIFVHVTINEIYGKWSPLILNPSYTILHFLGATKNRQFIGLKGFFNRSSKHSSVESNSHVRKKSIGDRILRRSASAPAKARKKTKMVFQESTESKDIVCETSGAKERDFMRKRTSRSLQARPVSMPVDKLLLGAFEPSTSEISKETRSDIASGSAGEMQAKHQEKPEMTKLDLPVHKNNLAIQHSSPLFKDCHQTENPVNKPVHKKWRSLHLEKQNYDDIRELNDTFTSESDEEILKPKEGNEKEAMLLLDKMESIPENTKHHAKADLFSVTDLSSTICTDMNHLQSTAPLLETDISHIISEVPSVNESELGSTVSALIRHFEITEDKTNLTFVSSLQGPNSQSNFCDNSDVLISDLLNRKKHTRQSLHSASNTTVFSSPETSEYSIYTFIHEEHVPPDANYKLMDANNGISSCSNLKNNSSNSWLDSSESLQFCSQYNANSNTLGLKSKQSRPQVSLKTSSGCTDENVKQISSHNSFLKNLNVDSNPKRIADKNFLHTGVNRYATQACPNSQNTGIQHGSGQLYQNRHSSYKPRADAPRDKQYIAQSGNLHSSPNANWPKPSNYHIHSLQGIKQPSPCISKSLGDLTSDDISCNFESKYKRINRGFLSSAICPENTSLAQSRPQSTDALTEQLRKLMSFDQDDSNRPSYYEEEEWDSPKPLTRKLSSRSQSRVRNIANRAKERQEANKIKQSNLNSSEVVLRNKRSMASQPINRHSTGSYIGSLYNNFNSERLEGRGIPEGACTSHTQVYNDHIDTGFTYRHEPSNTDEPEIYFLLRL

Target ORF information:

RefSeq Version	XM_031902336.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902336.1

ORF Insert Sequence:

ATGAGTTCCC AAAATCAGGA TAAGAGAAGA TTTCTGCAGT ACTGCCGCCA TTTCTTAGCT 
GACAATGGGG CATTTCATGT TGAAAGATGT ATGAGTGTAA TGCAGTCTGG GACACAGATG 
GTAAAACTAA AGAGTGGAAC AAAGGCTTTG GTTCGCCTCT TTTACCTGGA TGAACATCGG 
ACATGTATCA GATGGAGACC TTCAAGAAAA AGCGAGAAAG CTAAAATTAT AATTGATTCA 
ATTTACAAAG TCACGGAAGG GCGACAGTCT GAAATATTCC ACCGCCATGC AGAAGGAAAC 
TTTGATCCCA GTTGCTGTTT TACCATATAT CATGGAAACC ACATGGAATC TCTGGACCTC 
ATAACATCAA ATCCGGAGGA GGCCCGAACT TGGATCACAG GGCTGAAATA TCTGATGGCT 
GGAATTAGCG ATGAGGATTC TCTGGCAAAA AGGCAAAGGA CCCATGATCA ATGGGTGAAG 
CAGACATTTG AAGAGGCTGA TAAAAATGGG GATGGCTTAC TCAACATTGA GGAAATATAC 
CAGTTAATGC ATAAACTCAA CGTGAATCTG CCACGAAGGA AAGTTCGCCA GATGTTCCAG 
GAAGCAGACA CGGACGAGAA CCAAGGGACT CTGACCTTTG AAGAGTTTTC TGTGTTTTAC 
AAGATGATGT CATTACGGAG GGATTTGTAC CTGTTACTTT TAAACTACAG CGACAAAAAG 
GATCACTTAA CAGTTGACGA TCTGGCTCAG TTTTTCAAAG TAGAACAAAA GATGAATAAT 
GTGACAACAG AATACTGTAT TGACATTATA CAGAAATTTG AAGTCTCAGA GGAAAACAAA 
GAACAACATG TTCTGGGAAT AGATGGTTTT ACTAATTTCA TGAGAAGTCC TGCAGGAGAT 
ATTTTTAACC CTCAGCACTG TGAGATCTAC CAAGACATGG ACCAACCTCT CTGTAGCTAC 
TACATTGCAT CATCCCATAA CACCTACTTA ACTGGAGACC AACTTCTTTC TCAATCCAAA 
GTGGATATGT ATGCTTGGGT ATTGCAAGAT GGCTGTCGAT GCATAGAAGT TGACTGCTGG 
GATGGACCAG ATGGGGAGCC AGTGGTACAC CATGGCTATA CCCTTACTTC CAAAGTCCTT 
TTTAAAGATG TTGTGGAAAC CATCAACAAA CATGCTTTTG TGAAAAATGA ATATCCTGTT 
ATACTTTCAA TTGAAAACCA CTGCAACATT CAACAACAAA GAAAGATTGC TCAGTACATG 
AAAGATATAT TTGGGGATAA ACTTGATCTG TCTTCTGTCC CACTTGGAGA ATCCAAGCAA 
CTTCCTAGTC CTCAGAATCT CAAGGGAAAG ATATTAGTGA AGGGGAAAAA GTTGCCATAT 
GACCTGGCAG ACGATGCTGA GGAGGGAGAA GTCTCAGATG AAGATAGTGC TGACGAAATA 
GAAGAAGAAT GCAAACTCAC ACAATGTTTT AGTAATGGCA CAACAGAGCA CCAGGTGGAA 
TCTTTCATTC GGAAAAAACT GGAGTCTTTG CTAAAGGAAT CCCAGATCAG GGATAAGGAA 
GATCCAGATA GCTTTACCGT GAAAGCATTA CTAAAGGCAA CACATGAAGG CCTGAATGTT 
AATCTTAAAC AGAATGATGA CACAAAAGAT TGTGGAAAGT CTAAAAGGTC TTCAGTTGGA 
AGTATTGCCA AACACAAAAA AGCTTCCAAA TCCAGGTCTA AATCTTGCAG TACTAGTGAT 
GATGAAGATT CATCGCCAAA AGAGACAACA GCACAGGCAC ACAGGTGGAG CCAAGCTACA 
TATGGCATCT TGCTTACTTG TGCTTGGATT GGTGGAGAGG AAGATGTTGA ATATGCAAAT 
TACAGGTTGG GTCGTCGAAG GAAGACAATG AAGTTATGTC GAGCTTTGTC AGACTTGGTT 
GTCTACACAA ATTCTGTGGC TGCACAAGAC ATTGTAGATG ATGGGACCTC AGGAAACGTT 
TTATCCTTTA GTGAAACAAG AGCACACCAG GTGGTTCAGC ACAAATCTGA GCAGCTCATG 
TTGTACAATC AAAAACAATT AACTAGGATT TATCCATCTG CATACAGGAT AGACTCTAGT 
AATTTTAACC CAGTGGCCTA CTGGAATGCA GGAATTCAGC TTGTTGCGTT AAACTATCAA 
ACAGAAGGAC GCATGATGGA ATTAAATCGA GCTAAATTCA AGATGAATGG TGGTTGTGGA 
TATGTCCTTA AGCCCCAGCA GATGTGCAAA GGTGTATTTA ACCAATTTTC AGCTGACCCT 
CTTCCTGCCA GCCCAAAAAA GCAGTTAATT TTGAAGATAA TCAGTGGACA GCAACTTCCC 
AAACCACCAG ATTCAATGCT TGGGGATAGA GGAGAGATCA TTGACCCTTT TGTAGAAGTG 
GAAATTATCG GCTTGCCTGT GGACTGCTGT AAGGAACAGA CTCGAGTAGT TGATGACAAT 
GGTTTCAATC CTGTATGGGA GGAAACTCTT ACCTTTACAG TTCATATGCC AGAAATAGCA 
TTGGTAAGGT TCTTAGTATG GGACCATGAT CCAATTGGCC GGGATTTTGT TGGACAAAGG 
ACTGTAGCCT TTAACAGTCT TATGCCTGGA TATCGGCATG TTTATCTGGA TGGTCTAACA 
GAAGCCTCCA TATTTGTTCA TGTTACAATT AATGAAATAT ATGGAAAGTG GAGTCCTTTA 
ATCCTCAATC CCAGTTACAC AATATTGCAC TTTCTAGGAG CTACGAAGAA CAGGCAGTTT 
ATTGGCCTGA AAGGGTTTTT CAATAGGAGT TCTAAGCATA GCTCTGTTGA GAGCAATAGT 
CATGTTCGTA AGAAATCAAT TGGGGACCGT ATCTTGCGAC GCAGTGCGAG TGCTCCAGCC 
AAAGCCAGGA AGAAGACCAA GATGGTGTTT CAAGAGTCAA CTGAGAGTAA AGACATTGTC 
TGTGAAACAT CTGGTGCTAA AGAGAGAGAT TTTATGAGAA AGAGAACCTC AAGGAGTCTG 
CAAGCACGCC CTGTTTCAAT GCCTGTGGAT AAGTTACTAC TTGGGGCTTT TGAACCTTCA 
ACATCAGAGA TTTCTAAGGA GACTAGAAGT GACATAGCTT CAGGTTCAGC AGGGGAGATG 
CAAGCTAAGC ACCAGGAGAA GCCAGAGATG ACAAAACTGG ATCTACCAGT TCATAAAAAT 
AATCTTGCTA TTCAACATTC TTCCCCTCTT TTTAAAGATT GTCACCAGAC TGAAAATCCA 
GTAAATAAAC CTGTACATAA AAAATGGAGG AGCTTACATT TAGAAAAACA AAACTATGAT 
GATATTAGAG AGTTAAATGA TACATTTACC AGTGAATCTG ATGAGGAGAT TTTAAAGCCA 
AAAGAAGGTA ATGAAAAAGA AGCTATGCTG TTATTAGACA AAATGGAGTC CATCCCAGAA 
AACACCAAGC ATCATGCAAA AGCTGATTTA TTTTCTGTGA CTGATTTATC AAGCACTATA 
TGTACAGACA TGAATCACTT ACAGTCTACT GCACCTTTAT TAGAAACTGA TATTTCTCAT 
ATTATAAGTG AAGTTCCTTC AGTCAACGAG AGTGAACTTG GAAGCACGGT GTCTGCTTTA 
ATAAGGCACT TTGAGATCAC CGAAGACAAA ACTAATTTGA CATTTGTCTC AAGTTTGCAA 
GGTCCAAACA GCCAAAGCAA TTTTTGTGAC AATTCTGATG TGTTAATATC TGACCTTTTA 
AATAGAAAAA AGCACACAAG ACAATCTTTA CATTCAGCTT CCAACACTAC TGTGTTCTCC 
AGCCCTGAAA CAAGTGAATA TTCTATATAT ACATTTATTC ATGAAGAGCA TGTACCTCCT 
GATGCCAATT ACAAATTGAT GGATGCAAAC AATGGCATAT CATCGTGTTC TAATTTAAAA 
AATAATTCAT CAAACAGTTG GCTAGATAGT TCTGAGAGCC TACAGTTTTG TTCTCAATAT 
AATGCAAACA GCAATACATT AGGTCTAAAA TCAAAGCAAA GTAGGCCACA AGTTTCACTG 
AAAACATCAT CAGGCTGTAC AGATGAGAAT GTCAAACAGA TATCATCTCA CAACTCCTTT 
CTAAAGAACC TTAATGTAGA TAGTAATCCT AAGAGAATAG CGGATAAGAA TTTCTTACAT 
ACTGGTGTCA ATAGGTATGC CACACAAGCC TGTCCAAACT CTCAAAACAC AGGCATTCAA 
CACGGATCTG GTCAACTTTA TCAAAACAGG CATTCAAGTT ATAAACCAAG AGCAGATGCA 
CCACGAGACA AACAGTATAT TGCCCAGTCT GGAAACCTGC ACAGCTCGCC CAATGCAAAC 
TGGCCCAAAC CTTCAAACTA TCACATTCAT TCATTACAAG GTATTAAACA GCCAAGTCCA 
TGTATATCAA AAAGTCTTGG AGATCTGACA TCAGACGATA TATCTTGTAA TTTTGAAAGT 
AAGTACAAAC GGATCAACAG AGGGTTTCTT TCATCAGCTA TTTGTCCGGA AAATACATCA 
TTAGCTCAAA GCAGACCTCA GTCTACCGAT GCTTTAACGG AACAGTTGCG CAAACTGATG 
TCTTTTGACC AAGACGACAG TAACAGGCCA TCATATTATG AAGAAGAAGA ATGGGACAGT 
CCTAAGCCAC TTACAAGAAA GCTATCCTCA AGGAGTCAAA GTAGGGTCAG AAACATTGCT 
AACCGTGCCA AAGAAAGACA GGAGGCCAAT AAAATAAAAC AAAGCAATTT AAACTCAAGT 
GAAGTAGTCT TAAGAAACAA ACGTTCCATG GCTTCTCAGC CCATCAACAG GCATTCCACT 
GGTTCTTATA TAGGTAGCTT GTATAATAAC TTTAACAGTG AAAGGCTAGA AGGCAGAGGT 
ATACCAGAAG GTGCATGCAC TTCCCATACA CAAGTGTATA ATGACCACAT TGACACTGGC 
TTTACTTATC GACATGAGCC AAGCAATACT GATGAACCAG AAATTTATTT CCTACTAAGA 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plch1 ( XM_031902337.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758197.1 Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa58659
Clone ID Related Accession (Same CDS sequence)	XM_031902337.1
Accession Version	XM_031902337.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC TTGAAGTATA CAAAAATTTA AGCCCAGAAA AAGTTGAAAG ATGTATGAGT 
GTAATGCAGT CTGGGACACA GATGGTAAAA CTAAAGAGTG GAACAAAGGC TTTGGTTCGC 
CTCTTTTACC TGGATGAACA TCGGACATGT ATCAGATGGA GACCTTCAAG AAAAAGCGAG 
AAAGCTAAAA TTATAATTGA TTCAATTTAC AAAGTCACGG AAGGGCGACA GTCTGAAATA 
TTCCACCGCC ATGCAGAAGG AAACTTTGAT CCCAGTTGCT GTTTTACCAT ATATCATGGA 
AACCACATGG AATCTCTGGA CCTCATAACA TCAAATCCGG AGGAGGCCCG AACTTGGATC 
ACAGGGCTGA AATATCTGAT GGCTGGAATT AGCGATGAGG ATTCTCTGGC AAAAAGGCAA 
AGGACCCATG ATCAATGGGT GAAGCAGACA TTTGAAGAGG CTGATAAAAA TGGGGATGGC 
TTACTCAACA TTGAGGAAAT ATACCAGTTA ATGCATAAAC TCAACGTGAA TCTGCCACGA 
AGGAAAGTTC GCCAGATGTT CCAGGAAGCA GACACGGACG AGAACCAAGG GACTCTGACC 
TTTGAAGAGT TTTCTGTGTT TTACAAGATG ATGTCATTAC GGAGGGATTT GTACCTGTTA 
CTTTTAAACT ACAGCGACAA AAAGGATCAC TTAACAGTTG ACGATCTGGC TCAGTTTTTC 
AAAGTAGAAC AAAAGATGAA TAATGTGACA ACAGAATACT GTATTGACAT TATACAGAAA 
TTTGAAGTCT CAGAGGAAAA CAAAGAACAA CATGTTCTGG GAATAGATGG TTTTACTAAT 
TTCATGAGAA GTCCTGCAGG AGATATTTTT AACCCTCAGC ACTGTGAGAT CTACCAAGAC 
ATGGACCAAC CTCTCTGTAG CTACTACATT GCATCATCCC ATAACACCTA CTTAACTGGA 
GACCAACTTC TTTCTCAATC CAAAGTGGAT ATGTATGCTT GGGTATTGCA AGATGGCTGT 
CGATGCATAG AAGTTGACTG CTGGGATGGA CCAGATGGGG AGCCAGTGGT ACACCATGGC 
TATACCCTTA CTTCCAAAGT CCTTTTTAAA GATGTTGTGG AAACCATCAA CAAACATGCT 
TTTGTGAAAA ATGAATATCC TGTTATACTT TCAATTGAAA ACCACTGCAA CATTCAACAA 
CAAAGAAAGA TTGCTCAGTA CATGAAAGAT ATATTTGGGG ATAAACTTGA TCTGTCTTCT 
GTCCCACTTG GAGAATCCAA GCAACTTCCT AGTCCTCAGA ATCTCAAGGG AAAGATATTA 
GTGAAGGGGA AAAAGTTGCC ATATGACCTG GCAGACGATG CTGAGGAGGG AGAAGTCTCA 
GATGAAGATA GTGCTGACGA AATAGAAGAA GAATGCAAAC TCACACAATG TTTTAGTAAT 
GGCACAACAG AGCACCAGGT GGAATCTTTC ATTCGGAAAA AACTGGAGTC TTTGCTAAAG 
GAATCCCAGA TCAGGGATAA GGAAGATCCA GATAGCTTTA CCGTGAAAGC ATTACTAAAG 
GCAACACATG AAGGCCTGAA TGTTAATCTT AAACAGAATG ATGACACAAA AGATTGTGGA 
AAGTCTAAAA GGTCTTCAGT TGGAAGTATT GCCAAACACA AAAAAGCTTC CAAATCCAGG 
TCTAAATCTT GCAGTACTAG TGATGATGAA GATTCATCGC CAAAAGAGAC AACAGCACAG 
GCACACAGGT GGAGCCAAGC TACATATGGC ATCTTGCTTA CTTGTGCTTG GATTGGTGGA 
GAGGAAGATG TTGAATATGC AAATTACAGG TTGGGTCGTC GAAGGAAGAC AATGAAGTTA 
TGTCGAGCTT TGTCAGACTT GGTTGTCTAC ACAAATTCTG TGGCTGCACA AGACATTGTA 
GATGATGGGA CCTCAGGAAA CGTTTTATCC TTTAGTGAAA CAAGAGCACA CCAGGTGGTT 
CAGCACAAAT CTGAGCAGCT CATGTTGTAC AATCAAAAAC AATTAACTAG GATTTATCCA 
TCTGCATACA GGATAGACTC TAGTAATTTT AACCCAGTGG CCTACTGGAA TGCAGGAATT 
CAGCTTGTTG CGTTAAACTA TCAAACAGAA GGACGCATGA TGGAATTAAA TCGAGCTAAA 
TTCAAGATGA ATGGTGGTTG TGGATATGTC CTTAAGCCCC AGCAGATGTG CAAAGGTGTA 
TTTAACCAAT TTTCAGCTGA CCCTCTTCCT GCCAGCCCAA AAAAGCAGTT AATTTTGAAG 
ATAATCAGTG GACAGCAACT TCCCAAACCA CCAGATTCAA TGCTTGGGGA TAGAGGAGAG 
ATCATTGACC CTTTTGTAGA AGTGGAAATT ATCGGCTTGC CTGTGGACTG CTGTAAGGAA 
CAGACTCGAG TAGTTGATGA CAATGGTTTC AATCCTGTAT GGGAGGAAAC TCTTACCTTT 
ACAGTTCATA TGCCAGAAAT AGCATTGGTA AGGTTCTTAG TATGGGACCA TGATCCAATT 
GGCCGGGATT TTGTTGGACA AAGGACTGTA GCCTTTAACA GTCTTATGCC TGGATATCGG 
CATGTTTATC TGGATGGTCT AACAGAAGCC TCCATATTTG TTCATGTTAC AATTAATGAA 
ATATATGGAA AGTGGAGTCC TTTAATCCTC AATCCCAGTT ACACAATATT GCACTTTCTA 
GGAGCTACGA AGAACAGGCA GTTTATTGGC CTGAAAGGGT TTTTCAATAG GAGTTCTAAG 
CATAGCTCTG TTGAGAGCAA TAGTCATGTT CGTAAGAAAT CAATTGGGGA CCGTATCTTG 
CGACGCAGTG CGAGTGCTCC AGCCAAAGCC AGGAAGAAGA CCAAGATGGT GTTTCAAGAG 
TCAACTGAGA GTAAAGACAT TGTCTGTGAA ACATCTGGTG CTAAAGAGAG AGATTTTATG 
AGAAAGAGAA CCTCAAGGAG TCTGCAAGCA CGCCCTGTTT CAATGCCTGT GGATAAGTTA 
CTACTTGGGG CTTTTGAACC TTCAACATCA GAGATTTCTA AGGAGACTAG AAGTGACATA 
GCTTCAGGTT CAGCAGGGGA GATGCAAGCT AAGCACCAGG AGAAGCCAGA GATGACAAAA 
CTGGATCTAC CAGTTCATAA AAATAATCTT GCTATTCAAC ATTCTTCCCC TCTTTTTAAA 
GATTGTCACC AGACTGAAAA TCCAGTAAAT AAACCTGTAC ATAAAAAATG GAGGAGCTTA 
CATTTAGAAA AACAAAACTA TGATGATATT AGAGAGTTAA ATGATACATT TACCAGTGAA 
TCTGATGAGG AGATTTTAAA GCCAAAAGAA GGTAATGAAA AAGAAGCTAT GCTGTTATTA 
GACAAAATGG AGTCCATCCC AGAAAACACC AAGCATCATG CAAAAGCTGA TTTATTTTCT 
GTGACTGATT TATCAAGCAC TATATGTACA GACATGAATC ACTTACAGTC TACTGCACCT 
TTATTAGAAA CTGATATTTC TCATATTATA AGTGAAGTTC CTTCAGTCAA CGAGAGTGAA 
CTTGGAAGCA CGGTGTCTGC TTTAATAAGG CACTTTGAGA TCACCGAAGA CAAAACTAAT 
TTGACATTTG TCTCAAGTTT GCAAGGTCCA AACAGCCAAA GCAATTTTTG TGACAATTCT 
GATGTGTTAA TATCTGACCT TTTAAATAGA AAAAAGCACA CAAGACAATC TTTACATTCA 
GCTTCCAACA CTACTGTGTT CTCCAGCCCT GAAACAAGTG AATATTCTAT ATATACATTT 
ATTCATGAAG AGCATGTACC TCCTGATGCC AATTACAAAT TGATGGATGC AAACAATGGC 
ATATCATCGT GTTCTAATTT AAAAAATAAT TCATCAAACA GTTGGCTAGA TAGTTCTGAG 
AGCCTACAGT TTTGTTCTCA ATATAATGCA AACAGCAATA CATTAGGTCT AAAATCAAAG 
CAAAGTAGGC CACAAGTTTC ACTGAAAACA TCATCAGGCT GTACAGATGA GAATGTCAAA 
CAGATATCAT CTCACAACTC CTTTCTAAAG AACCTTAATG TAGATAGTAA TCCTAAGAGA 
ATAGCGGATA AGAATTTCTT ACATACTGGT GTCAATAGGT ATGCCACACA AGCCTGTCCA 
AACTCTCAAA ACACAGGCAT TCAACACGGA TCTGGTCAAC TTTATCAAAA CAGGCATTCA 
AGTTATAAAC CAAGAGCAGA TGCACCACGA GACAAACAGT ATATTGCCCA GTCTGGAAAC 
CTGCACAGCT CGCCCAATGC AAACTGGCCC AAACCTTCAA ACTATCACAT TCATTCATTA 
CAAGGTATTA AACAGCCAAG TCCATGTATA TCAAAAAGTC TTGGAGATCT GACATCAGAC 
GATATATCTT GTAATTTTGA AAGTAAGTAC AAACGGATCA ACAGAGGGTT TCTTTCATCA 
GCTATTTGTC CGGAAAATAC ATCATTAGCT CAAAGCAGAC CTCAGTCTAC CGATGCTTTA 
ACGGAACAGT TGCGCAAACT GATGTCTTTT GACCAAGACG ACAGTAACAG GCCATCATAT 
TATGAAGAAG AAGAATGGGA CAGTCCTAAG CCACTTACAA GAAAGCTATC CTCAAGGAGT 
CAAAGTAGGG TCAGAAACAT TGCTAACCGT GCCAAAGAAA GACAGGAGGC CAATAAAATA 
AAACAAAGCA ATTTAAACTC AAGTGAAGTA GTCTTAAGAA ACAAACGTTC CATGGCTTCT 
CAGCCCATCA ACAGGCATTC CACTGGTTCT TATATAGGTA GCTTGTATAA TAACTTTAAC 
AGTGAAAGGC TAGAAGGCAG AGGTATACCA GAAGGTGCAT GCACTTCCCA TACACAAGTG 
TATAATGACC ACATTGACAC TGGCTTTACT TATCGACATG AGCCAAGCAA TACTGATGAA 
CCAGAAATTT ATTTCCTACT AAGACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758197.1
CDS	222..5171
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMVKLKSGTKALVRLFYLDEHRTCIRWRPSRKSEKAKIIIDSIYKVTEGRQSEIFHRHAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIYQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFSVFYKMMSLRRDLYLLLLNYSDKKDHLTVDDLAQFFKVEQKMNNVTTEYCIDIIQKFEVSEENKEQHVLGIDGFTNFMRSPAGDIFNPQHCEIYQDMDQPLCSYYIASSHNTYLTGDQLLSQSKVDMYAWVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKVLFKDVVETINKHAFVKNEYPVILSIENHCNIQQQRKIAQYMKDIFGDKLDLSSVPLGESKQLPSPQNLKGKILVKGKKLPYDLADDAEEGEVSDEDSADEIEEECKLTQCFSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVKALLKATHEGLNVNLKQNDDTKDCGKSKRSSVGSIAKHKKASKSRSKSCSTSDDEDSSPKETTAQAHRWSQATYGILLTCAWIGGEEDVEYANYRLGRRRKTMKLCRALSDLVVYTNSVAAQDIVDDGTSGNVLSFSETRAHQVVQHKSEQLMLYNQKQLTRIYPSAYRIDSSNFNPVAYWNAGIQLVALNYQTEGRMMELNRAKFKMNGGCGYVLKPQQMCKGVFNQFSADPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKEQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVAFNSLMPGYRHVYLDGLTEASIFVHVTINEIYGKWSPLILNPSYTILHFLGATKNRQFIGLKGFFNRSSKHSSVESNSHVRKKSIGDRILRRSASAPAKARKKTKMVFQESTESKDIVCETSGAKERDFMRKRTSRSLQARPVSMPVDKLLLGAFEPSTSEISKETRSDIASGSAGEMQAKHQEKPEMTKLDLPVHKNNLAIQHSSPLFKDCHQTENPVNKPVHKKWRSLHLEKQNYDDIRELNDTFTSESDEEILKPKEGNEKEAMLLLDKMESIPENTKHHAKADLFSVTDLSSTICTDMNHLQSTAPLLETDISHIISEVPSVNESELGSTVSALIRHFEITEDKTNLTFVSSLQGPNSQSNFCDNSDVLISDLLNRKKHTRQSLHSASNTTVFSSPETSEYSIYTFIHEEHVPPDANYKLMDANNGISSCSNLKNNSSNSWLDSSESLQFCSQYNANSNTLGLKSKQSRPQVSLKTSSGCTDENVKQISSHNSFLKNLNVDSNPKRIADKNFLHTGVNRYATQACPNSQNTGIQHGSGQLYQNRHSSYKPRADAPRDKQYIAQSGNLHSSPNANWPKPSNYHIHSLQGIKQPSPCISKSLGDLTSDDISCNFESKYKRINRGFLSSAICPENTSLAQSRPQSTDALTEQLRKLMSFDQDDSNRPSYYEEEEWDSPKPLTRKLSSRSQSRVRNIANRAKERQEANKIKQSNLNSSEVVLRNKRSMASQPINRHSTGSYIGSLYNNFNSERLEGRGIPEGACTSHTQVYNDHIDTGFTYRHEPSNTDEPEIYFLLRL

Target ORF information:

RefSeq Version	XM_031902337.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902337.1

ORF Insert Sequence:

ATGGCAGACC TTGAAGTATA CAAAAATTTA AGCCCAGAAA AAGTTGAAAG ATGTATGAGT 
GTAATGCAGT CTGGGACACA GATGGTAAAA CTAAAGAGTG GAACAAAGGC TTTGGTTCGC 
CTCTTTTACC TGGATGAACA TCGGACATGT ATCAGATGGA GACCTTCAAG AAAAAGCGAG 
AAAGCTAAAA TTATAATTGA TTCAATTTAC AAAGTCACGG AAGGGCGACA GTCTGAAATA 
TTCCACCGCC ATGCAGAAGG AAACTTTGAT CCCAGTTGCT GTTTTACCAT ATATCATGGA 
AACCACATGG AATCTCTGGA CCTCATAACA TCAAATCCGG AGGAGGCCCG AACTTGGATC 
ACAGGGCTGA AATATCTGAT GGCTGGAATT AGCGATGAGG ATTCTCTGGC AAAAAGGCAA 
AGGACCCATG ATCAATGGGT GAAGCAGACA TTTGAAGAGG CTGATAAAAA TGGGGATGGC 
TTACTCAACA TTGAGGAAAT ATACCAGTTA ATGCATAAAC TCAACGTGAA TCTGCCACGA 
AGGAAAGTTC GCCAGATGTT CCAGGAAGCA GACACGGACG AGAACCAAGG GACTCTGACC 
TTTGAAGAGT TTTCTGTGTT TTACAAGATG ATGTCATTAC GGAGGGATTT GTACCTGTTA 
CTTTTAAACT ACAGCGACAA AAAGGATCAC TTAACAGTTG ACGATCTGGC TCAGTTTTTC 
AAAGTAGAAC AAAAGATGAA TAATGTGACA ACAGAATACT GTATTGACAT TATACAGAAA 
TTTGAAGTCT CAGAGGAAAA CAAAGAACAA CATGTTCTGG GAATAGATGG TTTTACTAAT 
TTCATGAGAA GTCCTGCAGG AGATATTTTT AACCCTCAGC ACTGTGAGAT CTACCAAGAC 
ATGGACCAAC CTCTCTGTAG CTACTACATT GCATCATCCC ATAACACCTA CTTAACTGGA 
GACCAACTTC TTTCTCAATC CAAAGTGGAT ATGTATGCTT GGGTATTGCA AGATGGCTGT 
CGATGCATAG AAGTTGACTG CTGGGATGGA CCAGATGGGG AGCCAGTGGT ACACCATGGC 
TATACCCTTA CTTCCAAAGT CCTTTTTAAA GATGTTGTGG AAACCATCAA CAAACATGCT 
TTTGTGAAAA ATGAATATCC TGTTATACTT TCAATTGAAA ACCACTGCAA CATTCAACAA 
CAAAGAAAGA TTGCTCAGTA CATGAAAGAT ATATTTGGGG ATAAACTTGA TCTGTCTTCT 
GTCCCACTTG GAGAATCCAA GCAACTTCCT AGTCCTCAGA ATCTCAAGGG AAAGATATTA 
GTGAAGGGGA AAAAGTTGCC ATATGACCTG GCAGACGATG CTGAGGAGGG AGAAGTCTCA 
GATGAAGATA GTGCTGACGA AATAGAAGAA GAATGCAAAC TCACACAATG TTTTAGTAAT 
GGCACAACAG AGCACCAGGT GGAATCTTTC ATTCGGAAAA AACTGGAGTC TTTGCTAAAG 
GAATCCCAGA TCAGGGATAA GGAAGATCCA GATAGCTTTA CCGTGAAAGC ATTACTAAAG 
GCAACACATG AAGGCCTGAA TGTTAATCTT AAACAGAATG ATGACACAAA AGATTGTGGA 
AAGTCTAAAA GGTCTTCAGT TGGAAGTATT GCCAAACACA AAAAAGCTTC CAAATCCAGG 
TCTAAATCTT GCAGTACTAG TGATGATGAA GATTCATCGC CAAAAGAGAC AACAGCACAG 
GCACACAGGT GGAGCCAAGC TACATATGGC ATCTTGCTTA CTTGTGCTTG GATTGGTGGA 
GAGGAAGATG TTGAATATGC AAATTACAGG TTGGGTCGTC GAAGGAAGAC AATGAAGTTA 
TGTCGAGCTT TGTCAGACTT GGTTGTCTAC ACAAATTCTG TGGCTGCACA AGACATTGTA 
GATGATGGGA CCTCAGGAAA CGTTTTATCC TTTAGTGAAA CAAGAGCACA CCAGGTGGTT 
CAGCACAAAT CTGAGCAGCT CATGTTGTAC AATCAAAAAC AATTAACTAG GATTTATCCA 
TCTGCATACA GGATAGACTC TAGTAATTTT AACCCAGTGG CCTACTGGAA TGCAGGAATT 
CAGCTTGTTG CGTTAAACTA TCAAACAGAA GGACGCATGA TGGAATTAAA TCGAGCTAAA 
TTCAAGATGA ATGGTGGTTG TGGATATGTC CTTAAGCCCC AGCAGATGTG CAAAGGTGTA 
TTTAACCAAT TTTCAGCTGA CCCTCTTCCT GCCAGCCCAA AAAAGCAGTT AATTTTGAAG 
ATAATCAGTG GACAGCAACT TCCCAAACCA CCAGATTCAA TGCTTGGGGA TAGAGGAGAG 
ATCATTGACC CTTTTGTAGA AGTGGAAATT ATCGGCTTGC CTGTGGACTG CTGTAAGGAA 
CAGACTCGAG TAGTTGATGA CAATGGTTTC AATCCTGTAT GGGAGGAAAC TCTTACCTTT 
ACAGTTCATA TGCCAGAAAT AGCATTGGTA AGGTTCTTAG TATGGGACCA TGATCCAATT 
GGCCGGGATT TTGTTGGACA AAGGACTGTA GCCTTTAACA GTCTTATGCC TGGATATCGG 
CATGTTTATC TGGATGGTCT AACAGAAGCC TCCATATTTG TTCATGTTAC AATTAATGAA 
ATATATGGAA AGTGGAGTCC TTTAATCCTC AATCCCAGTT ACACAATATT GCACTTTCTA 
GGAGCTACGA AGAACAGGCA GTTTATTGGC CTGAAAGGGT TTTTCAATAG GAGTTCTAAG 
CATAGCTCTG TTGAGAGCAA TAGTCATGTT CGTAAGAAAT CAATTGGGGA CCGTATCTTG 
CGACGCAGTG CGAGTGCTCC AGCCAAAGCC AGGAAGAAGA CCAAGATGGT GTTTCAAGAG 
TCAACTGAGA GTAAAGACAT TGTCTGTGAA ACATCTGGTG CTAAAGAGAG AGATTTTATG 
AGAAAGAGAA CCTCAAGGAG TCTGCAAGCA CGCCCTGTTT CAATGCCTGT GGATAAGTTA 
CTACTTGGGG CTTTTGAACC TTCAACATCA GAGATTTCTA AGGAGACTAG AAGTGACATA 
GCTTCAGGTT CAGCAGGGGA GATGCAAGCT AAGCACCAGG AGAAGCCAGA GATGACAAAA 
CTGGATCTAC CAGTTCATAA AAATAATCTT GCTATTCAAC ATTCTTCCCC TCTTTTTAAA 
GATTGTCACC AGACTGAAAA TCCAGTAAAT AAACCTGTAC ATAAAAAATG GAGGAGCTTA 
CATTTAGAAA AACAAAACTA TGATGATATT AGAGAGTTAA ATGATACATT TACCAGTGAA 
TCTGATGAGG AGATTTTAAA GCCAAAAGAA GGTAATGAAA AAGAAGCTAT GCTGTTATTA 
GACAAAATGG AGTCCATCCC AGAAAACACC AAGCATCATG CAAAAGCTGA TTTATTTTCT 
GTGACTGATT TATCAAGCAC TATATGTACA GACATGAATC ACTTACAGTC TACTGCACCT 
TTATTAGAAA CTGATATTTC TCATATTATA AGTGAAGTTC CTTCAGTCAA CGAGAGTGAA 
CTTGGAAGCA CGGTGTCTGC TTTAATAAGG CACTTTGAGA TCACCGAAGA CAAAACTAAT 
TTGACATTTG TCTCAAGTTT GCAAGGTCCA AACAGCCAAA GCAATTTTTG TGACAATTCT 
GATGTGTTAA TATCTGACCT TTTAAATAGA AAAAAGCACA CAAGACAATC TTTACATTCA 
GCTTCCAACA CTACTGTGTT CTCCAGCCCT GAAACAAGTG AATATTCTAT ATATACATTT 
ATTCATGAAG AGCATGTACC TCCTGATGCC AATTACAAAT TGATGGATGC AAACAATGGC 
ATATCATCGT GTTCTAATTT AAAAAATAAT TCATCAAACA GTTGGCTAGA TAGTTCTGAG 
AGCCTACAGT TTTGTTCTCA ATATAATGCA AACAGCAATA CATTAGGTCT AAAATCAAAG 
CAAAGTAGGC CACAAGTTTC ACTGAAAACA TCATCAGGCT GTACAGATGA GAATGTCAAA 
CAGATATCAT CTCACAACTC CTTTCTAAAG AACCTTAATG TAGATAGTAA TCCTAAGAGA 
ATAGCGGATA AGAATTTCTT ACATACTGGT GTCAATAGGT ATGCCACACA AGCCTGTCCA 
AACTCTCAAA ACACAGGCAT TCAACACGGA TCTGGTCAAC TTTATCAAAA CAGGCATTCA 
AGTTATAAAC CAAGAGCAGA TGCACCACGA GACAAACAGT ATATTGCCCA GTCTGGAAAC 
CTGCACAGCT CGCCCAATGC AAACTGGCCC AAACCTTCAA ACTATCACAT TCATTCATTA 
CAAGGTATTA AACAGCCAAG TCCATGTATA TCAAAAAGTC TTGGAGATCT GACATCAGAC 
GATATATCTT GTAATTTTGA AAGTAAGTAC AAACGGATCA ACAGAGGGTT TCTTTCATCA 
GCTATTTGTC CGGAAAATAC ATCATTAGCT CAAAGCAGAC CTCAGTCTAC CGATGCTTTA 
ACGGAACAGT TGCGCAAACT GATGTCTTTT GACCAAGACG ACAGTAACAG GCCATCATAT 
TATGAAGAAG AAGAATGGGA CAGTCCTAAG CCACTTACAA GAAAGCTATC CTCAAGGAGT 
CAAAGTAGGG TCAGAAACAT TGCTAACCGT GCCAAAGAAA GACAGGAGGC CAATAAAATA 
AAACAAAGCA ATTTAAACTC AAGTGAAGTA GTCTTAAGAA ACAAACGTTC CATGGCTTCT 
CAGCCCATCA ACAGGCATTC CACTGGTTCT TATATAGGTA GCTTGTATAA TAACTTTAAC 
AGTGAAAGGC TAGAAGGCAG AGGTATACCA GAAGGTGCAT GCACTTCCCA TACACAAGTG 
TATAATGACC ACATTGACAC TGGCTTTACT TATCGACATG AGCCAAGCAA TACTGATGAA 
CCAGAAATTT ATTTCCTACT AAGACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plch1 ( XM_031902338.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758198.1 Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa58660
Clone ID Related Accession (Same CDS sequence)	XM_031902338.1
Accession Version	XM_031902338.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCC AAAATCAGGA TAAGAGAAGA TTTCTGCAGT ACTGCCGCCA TTTCTTAGCT 
GACAATGGGG CATTTCATGT TGAAAGATGT ATGAGTGTAA TGCAGTCTGG GACACAGATG 
GTAAAACTAA AGAGTGGAAC AAAGGCTTTG GTTCGCCTCT TTTACCTGGA TGAACATCGG 
ACATGTATCA GATGGAGACC TTCAAGAAAA AGCGAGAAAG CTAAAATTAT AATTGATTCA 
ATTTACAAAG TCACGGAAGG GCGACAGTCT GAAATATTCC ACCGCCATGC AGAAGGAAAC 
TTTGATCCCA GTTGCTGTTT TACCATATAT CATGGAAACC ACATGGAATC TCTGGACCTC 
ATAACATCAA ATCCGGAGGA GGCCCGAACT TGGATCACAG GGCTGAAATA TCTGATGGCT 
GGAATTAGCG ATGAGGATTC TCTGGCAAAA AGGCAAAGGA CCCATGATCA ATGGGTGAAG 
CAGACATTTG AAGAGGCTGA TAAAAATGGG GATGGCTTAC TCAACATTGA GGAAATATAC 
CAGTTAATGC ATAAACTCAA CGTGAATCTG CCACGAAGGA AAGTTCGCCA GATGTTCCAG 
GAAGCAGACA CGGACGAGAA CCAAGGGACT CTGACCTTTG AAGAGTTTTC TGTGTTTTAC 
AAGATGATGT CATTACGGAG GGATTTGTAC CTGTTACTTT TAAACTACAG CGACAAAAAG 
GATCACTTAA CAGTTGACGA TCTGGCTCAG TTTTTCAAAG TAGAACAAAA GATGAATAAT 
GTGACAACAG AATACTGTAT TGACATTATA CAGAAATTTG AAGTCTCAGA GGAAAACAAA 
GAACAACATG TTCTGGGAAT AGATGGTTTT ACTAATTTCA TGAGAAGTCC TGCAGGAGAT 
ATTTTTAACC CTCAGCACTG TGAGATCTAC CAAGACATGG ACCAACCTCT CTGTAGCTAC 
TACATTGCAT CATCCCATAA CACCTACTTA ACTGGAGACC AACTTCTTTC TCAATCCAAA 
GTGGATATGT ATGCTTGGGT ATTGCAAGAT GGCTGTCGAT GCATAGAAGT TGACTGCTGG 
GATGGACCAG ATGGGGAGCC AGTGGTACAC CATGGCTATA CCCTTACTTC CAAAGTCCTT 
TTTAAAGATG TTGTGGAAAC CATCAACAAA CATGCTTTTG TGAAAAATGA ATATCCTGTT 
ATACTTTCAA TTGAAAACCA CTGCAACATT CAACAACAAA GAAAGATTGC TCAGTACATG 
AAAGATATAT TTGGGGATAA ACTTGATCTG TCTTCTGTCC CACTTGGAGA ATCCAAGCAA 
CTTCCTAGTC CTCAGAATCT CAAGGGAAAG ATATTAGTGA AGGGGAAAAA GTTGCCATAT 
GACCTGGCAG ACGATGCTGA GGAGGGAGAA GTCTCAGATG AAGATAGTGC TGACGAAATA 
GAAGAAGAAT GCAAACTCAC ACAATGTTTT AGTAATGGCA CAACAGAGCA CCAGGTGGAA 
TCTTTCATTC GGAAAAAACT GGAGTCTTTG CTAAAGGAAT CCCAGATCAG GGATAAGGAA 
GATCCAGATA GCTTTACCGT GAAAGCATTA CTAAAGGCAA CACATGAAGG CCTGAATGTT 
AATCTTAAAC AGAATGATGA CACAAAAGAT TGTGGAAAGT CTAAAAGGTC TTCAGTTGGA 
AGTATTGCCA AACACAAAAA AGCTTCCAAA TCCAGGTCTA AATCTTGCAG TACTAGTGAT 
GATGAAGATT CATCGCCAAA AGAGACAACA GCACAGGCAC ACAGGTGGAG CCAAGCTACA 
TATGGCATCT TGCTTACTTG TGCTTGGATT GGTGGAGAGG AAGATGTTGA ATATGCAAAT 
TACAGGTTGG GTCGTCGAAG GAAGACAATG AAGTTATGTC GAGCTTTGTC AGACTTGGTT 
GTCTACACAA ATTCTGTGGC TGCACAAGAC ATTGTAGATG ATGGGACCTC AGGAAACGTT 
TTATCCTTTA GTGAAACAAG AGCACACCAG GTGGTTCAGC ACAAATCTGA GCAGCTCATG 
TTGTACAATC AAAAACAATT AACTAGGATT TATCCATCTG CATACAGGAT AGACTCTAGT 
AATTTTAACC CAGTGGCCTA CTGGAATGCA GGAATTCAGC TTGTTGCGTT AAACTATCAA 
ACAGAAGGAC GCATGATGGA ATTAAATCGA GCTAAATTCA AGATGAATGG TGGTTGTGGA 
TATGTCCTTA AGCCCCAGCA GATGTGCAAA GGTGTATTTA ACCAATTTTC AGCTGACCCT 
CTTCCTGCCA GCCCAAAAAA GCAGTTAATT TTGAAGATAA TCAGTGGACA GCAACTTCCC 
AAACCACCAG ATTCAATGCT TGGGGATAGA GGAGAGATCA TTGACCCTTT TGTAGAAGTG 
GAAATTATCG GCTTGCCTGT GGACTGCTGT AAGGAACAGA CTCGAGTAGT TGATGACAAT 
GGTTTCAATC CTGTATGGGA GGAAACTCTT ACCTTTACAG TTCATATGCC AGAAATAGCA 
TTGGTAAGGT TCTTAGTATG GGACCATGAT CCAATTGGCC GGGATTTTGT TGGACAAAGG 
ACTGTAGCCT TTAACAGTCT TATGCCTGGA TATCGGCATG TTTATCTGGA TGGTCTAACA 
GAAGCCTCCA TATTTGTTCA TGTTACAATT AATGAAATAT ATGGAAAGAA CAGGCAGTTT 
ATTGGCCTGA AAGGGTTTTT CAATAGGAGT TCTAAGCATA GCTCTGTTGA GAGCAATAGT 
CATGTTCGTA AGAAATCAAT TGGGGACCGT ATCTTGCGAC GCAGTGCGAG TGCTCCAGCC 
AAAGCCAGGA AGAAGACCAA GATGGTGTTT CAAGAGTCAA CTGAGAGTAA AGACATTGTC 
TGTGAAACAT CTGGTGCTAA AGAGAGAGAT TTTATGAGAA AGAGAACCTC AAGGAGTCTG 
CAAGCACGCC CTGTTTCAAT GCCTGTGGAT AAGTTACTAC TTGGGGCTTT TGAACCTTCA 
ACATCAGAGA TTTCTAAGGA GACTAGAAGT GACATAGCTT CAGGTTCAGC AGGGGAGATG 
CAAGCTAAGC ACCAGGAGAA GCCAGAGATG ACAAAACTGG ATCTACCAGT TCATAAAAAT 
AATCTTGCTA TTCAACATTC TTCCCCTCTT TTTAAAGATT GTCACCAGAC TGAAAATCCA 
GTAAATAAAC CTGTACATAA AAAATGGAGG AGCTTACATT TAGAAAAACA AAACTATGAT 
GATATTAGAG AGTTAAATGA TACATTTACC AGTGAATCTG ATGAGGAGAT TTTAAAGCCA 
AAAGAAGGTA ATGAAAAAGA AGCTATGCTG TTATTAGACA AAATGGAGTC CATCCCAGAA 
AACACCAAGC ATCATGCAAA AGCTGATTTA TTTTCTGTGA CTGATTTATC AAGCACTATA 
TGTACAGACA TGAATCACTT ACAGTCTACT GCACCTTTAT TAGAAACTGA TATTTCTCAT 
ATTATAAGTG AAGTTCCTTC AGTCAACGAG AGTGAACTTG GAAGCACGGT GTCTGCTTTA 
ATAAGGCACT TTGAGATCAC CGAAGACAAA ACTAATTTGA CATTTGTCTC AAGTTTGCAA 
GGTCCAAACA GCCAAAGCAA TTTTTGTGAC AATTCTGATG TGTTAATATC TGACCTTTTA 
AATAGAAAAA AGCACACAAG ACAATCTTTA CATTCAGCTT CCAACACTAC TGTGTTCTCC 
AGCCCTGAAA CAAGTGAATA TTCTATATAT ACATTTATTC ATGAAGAGCA TGTACCTCCT 
GATGCCAATT ACAAATTGAT GGATGCAAAC AATGGCATAT CATCGTGTTC TAATTTAAAA 
AATAATTCAT CAAACAGTTG GCTAGATAGT TCTGAGAGCC TACAGTTTTG TTCTCAATAT 
AATGCAAACA GCAATACATT AGGTCTAAAA TCAAAGCAAA GTAGGCCACA AGTTTCACTG 
AAAACATCAT CAGGCTGTAC AGATGAGAAT GTCAAACAGA TATCATCTCA CAACTCCTTT 
CTAAAGAACC TTAATGTAGA TAGTAATCCT AAGAGAATAG CGGATAAGAA TTTCTTACAT 
ACTGGTGTCA ATAGGTATGC CACACAAGCC TGTCCAAACT CTCAAAACAC AGGCATTCAA 
CACGGATCTG GTCAACTTTA TCAAAACAGG CATTCAAGTT ATAAACCAAG AGCAGATGCA 
CCACGAGACA AACAGTATAT TGCCCAGTCT GGAAACCTGC ACAGCTCGCC CAATGCAAAC 
TGGCCCAAAC CTTCAAACTA TCACATTCAT TCATTACAAG GTATTAAACA GCCAAGTCCA 
TGTATATCAA AAAGTCTTGG AGATCTGACA TCAGACGATA TATCTTGTAA TTTTGAAAGT 
AAGTACAAAC GGATCAACAG AGGGTTTCTT TCATCAGCTA TTTGTCCGGA AAATACATCA 
TTAGCTCAAA GCAGACCTCA GTCTACCGAT GCTTTAACGG AACAGTTGCG CAAACTGATG 
TCTTTTGACC AAGACGACAG TAACAGGCCA TCATATTATG AAGAAGAAGA ATGGGACAGT 
CCTAAGCCAC TTACAAGAAA GCTATCCTCA AGGAGTCAAA GTAGGGTCAG AAACATTGCT 
AACCGTGCCA AAGAAAGACA GGAGGCCAAT AAAATAAAAC AAAGCAATTT AAACTCAAGT 
GAAGTAGTCT TAAGAAACAA ACGTTCCATG GCTTCTCAGC CCATCAACAG GCATTCCACT 
GGTTCTTATA TAGGTAGCTT GTATAATAAC TTTAACAGTG AAAGGCTAGA AGGCAGAGGT 
ATACCAGAAG GTGCATGCAC TTCCCATACA CAAGTGTATA ATGACCACAT TGACACTGGC 
TTTACTTATC GACATGAGCC AAGCAATACT GATGAACCAG AAATTTATTT CCTACTAAGA 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758198.1
CDS	249..5174
Translation	MSSQNQDKRRFLQYCRHFLADNGAFHVERCMSVMQSGTQMVKLKSGTKALVRLFYLDEHRTCIRWRPSRKSEKAKIIIDSIYKVTEGRQSEIFHRHAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIYQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFSVFYKMMSLRRDLYLLLLNYSDKKDHLTVDDLAQFFKVEQKMNNVTTEYCIDIIQKFEVSEENKEQHVLGIDGFTNFMRSPAGDIFNPQHCEIYQDMDQPLCSYYIASSHNTYLTGDQLLSQSKVDMYAWVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKVLFKDVVETINKHAFVKNEYPVILSIENHCNIQQQRKIAQYMKDIFGDKLDLSSVPLGESKQLPSPQNLKGKILVKGKKLPYDLADDAEEGEVSDEDSADEIEEECKLTQCFSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVKALLKATHEGLNVNLKQNDDTKDCGKSKRSSVGSIAKHKKASKSRSKSCSTSDDEDSSPKETTAQAHRWSQATYGILLTCAWIGGEEDVEYANYRLGRRRKTMKLCRALSDLVVYTNSVAAQDIVDDGTSGNVLSFSETRAHQVVQHKSEQLMLYNQKQLTRIYPSAYRIDSSNFNPVAYWNAGIQLVALNYQTEGRMMELNRAKFKMNGGCGYVLKPQQMCKGVFNQFSADPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKEQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVAFNSLMPGYRHVYLDGLTEASIFVHVTINEIYGKNRQFIGLKGFFNRSSKHSSVESNSHVRKKSIGDRILRRSASAPAKARKKTKMVFQESTESKDIVCETSGAKERDFMRKRTSRSLQARPVSMPVDKLLLGAFEPSTSEISKETRSDIASGSAGEMQAKHQEKPEMTKLDLPVHKNNLAIQHSSPLFKDCHQTENPVNKPVHKKWRSLHLEKQNYDDIRELNDTFTSESDEEILKPKEGNEKEAMLLLDKMESIPENTKHHAKADLFSVTDLSSTICTDMNHLQSTAPLLETDISHIISEVPSVNESELGSTVSALIRHFEITEDKTNLTFVSSLQGPNSQSNFCDNSDVLISDLLNRKKHTRQSLHSASNTTVFSSPETSEYSIYTFIHEEHVPPDANYKLMDANNGISSCSNLKNNSSNSWLDSSESLQFCSQYNANSNTLGLKSKQSRPQVSLKTSSGCTDENVKQISSHNSFLKNLNVDSNPKRIADKNFLHTGVNRYATQACPNSQNTGIQHGSGQLYQNRHSSYKPRADAPRDKQYIAQSGNLHSSPNANWPKPSNYHIHSLQGIKQPSPCISKSLGDLTSDDISCNFESKYKRINRGFLSSAICPENTSLAQSRPQSTDALTEQLRKLMSFDQDDSNRPSYYEEEEWDSPKPLTRKLSSRSQSRVRNIANRAKERQEANKIKQSNLNSSEVVLRNKRSMASQPINRHSTGSYIGSLYNNFNSERLEGRGIPEGACTSHTQVYNDHIDTGFTYRHEPSNTDEPEIYFLLRL

Target ORF information:

RefSeq Version	XM_031902338.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902338.1

ORF Insert Sequence:

ATGAGTTCCC AAAATCAGGA TAAGAGAAGA TTTCTGCAGT ACTGCCGCCA TTTCTTAGCT 
GACAATGGGG CATTTCATGT TGAAAGATGT ATGAGTGTAA TGCAGTCTGG GACACAGATG 
GTAAAACTAA AGAGTGGAAC AAAGGCTTTG GTTCGCCTCT TTTACCTGGA TGAACATCGG 
ACATGTATCA GATGGAGACC TTCAAGAAAA AGCGAGAAAG CTAAAATTAT AATTGATTCA 
ATTTACAAAG TCACGGAAGG GCGACAGTCT GAAATATTCC ACCGCCATGC AGAAGGAAAC 
TTTGATCCCA GTTGCTGTTT TACCATATAT CATGGAAACC ACATGGAATC TCTGGACCTC 
ATAACATCAA ATCCGGAGGA GGCCCGAACT TGGATCACAG GGCTGAAATA TCTGATGGCT 
GGAATTAGCG ATGAGGATTC TCTGGCAAAA AGGCAAAGGA CCCATGATCA ATGGGTGAAG 
CAGACATTTG AAGAGGCTGA TAAAAATGGG GATGGCTTAC TCAACATTGA GGAAATATAC 
CAGTTAATGC ATAAACTCAA CGTGAATCTG CCACGAAGGA AAGTTCGCCA GATGTTCCAG 
GAAGCAGACA CGGACGAGAA CCAAGGGACT CTGACCTTTG AAGAGTTTTC TGTGTTTTAC 
AAGATGATGT CATTACGGAG GGATTTGTAC CTGTTACTTT TAAACTACAG CGACAAAAAG 
GATCACTTAA CAGTTGACGA TCTGGCTCAG TTTTTCAAAG TAGAACAAAA GATGAATAAT 
GTGACAACAG AATACTGTAT TGACATTATA CAGAAATTTG AAGTCTCAGA GGAAAACAAA 
GAACAACATG TTCTGGGAAT AGATGGTTTT ACTAATTTCA TGAGAAGTCC TGCAGGAGAT 
ATTTTTAACC CTCAGCACTG TGAGATCTAC CAAGACATGG ACCAACCTCT CTGTAGCTAC 
TACATTGCAT CATCCCATAA CACCTACTTA ACTGGAGACC AACTTCTTTC TCAATCCAAA 
GTGGATATGT ATGCTTGGGT ATTGCAAGAT GGCTGTCGAT GCATAGAAGT TGACTGCTGG 
GATGGACCAG ATGGGGAGCC AGTGGTACAC CATGGCTATA CCCTTACTTC CAAAGTCCTT 
TTTAAAGATG TTGTGGAAAC CATCAACAAA CATGCTTTTG TGAAAAATGA ATATCCTGTT 
ATACTTTCAA TTGAAAACCA CTGCAACATT CAACAACAAA GAAAGATTGC TCAGTACATG 
AAAGATATAT TTGGGGATAA ACTTGATCTG TCTTCTGTCC CACTTGGAGA ATCCAAGCAA 
CTTCCTAGTC CTCAGAATCT CAAGGGAAAG ATATTAGTGA AGGGGAAAAA GTTGCCATAT 
GACCTGGCAG ACGATGCTGA GGAGGGAGAA GTCTCAGATG AAGATAGTGC TGACGAAATA 
GAAGAAGAAT GCAAACTCAC ACAATGTTTT AGTAATGGCA CAACAGAGCA CCAGGTGGAA 
TCTTTCATTC GGAAAAAACT GGAGTCTTTG CTAAAGGAAT CCCAGATCAG GGATAAGGAA 
GATCCAGATA GCTTTACCGT GAAAGCATTA CTAAAGGCAA CACATGAAGG CCTGAATGTT 
AATCTTAAAC AGAATGATGA CACAAAAGAT TGTGGAAAGT CTAAAAGGTC TTCAGTTGGA 
AGTATTGCCA AACACAAAAA AGCTTCCAAA TCCAGGTCTA AATCTTGCAG TACTAGTGAT 
GATGAAGATT CATCGCCAAA AGAGACAACA GCACAGGCAC ACAGGTGGAG CCAAGCTACA 
TATGGCATCT TGCTTACTTG TGCTTGGATT GGTGGAGAGG AAGATGTTGA ATATGCAAAT 
TACAGGTTGG GTCGTCGAAG GAAGACAATG AAGTTATGTC GAGCTTTGTC AGACTTGGTT 
GTCTACACAA ATTCTGTGGC TGCACAAGAC ATTGTAGATG ATGGGACCTC AGGAAACGTT 
TTATCCTTTA GTGAAACAAG AGCACACCAG GTGGTTCAGC ACAAATCTGA GCAGCTCATG 
TTGTACAATC AAAAACAATT AACTAGGATT TATCCATCTG CATACAGGAT AGACTCTAGT 
AATTTTAACC CAGTGGCCTA CTGGAATGCA GGAATTCAGC TTGTTGCGTT AAACTATCAA 
ACAGAAGGAC GCATGATGGA ATTAAATCGA GCTAAATTCA AGATGAATGG TGGTTGTGGA 
TATGTCCTTA AGCCCCAGCA GATGTGCAAA GGTGTATTTA ACCAATTTTC AGCTGACCCT 
CTTCCTGCCA GCCCAAAAAA GCAGTTAATT TTGAAGATAA TCAGTGGACA GCAACTTCCC 
AAACCACCAG ATTCAATGCT TGGGGATAGA GGAGAGATCA TTGACCCTTT TGTAGAAGTG 
GAAATTATCG GCTTGCCTGT GGACTGCTGT AAGGAACAGA CTCGAGTAGT TGATGACAAT 
GGTTTCAATC CTGTATGGGA GGAAACTCTT ACCTTTACAG TTCATATGCC AGAAATAGCA 
TTGGTAAGGT TCTTAGTATG GGACCATGAT CCAATTGGCC GGGATTTTGT TGGACAAAGG 
ACTGTAGCCT TTAACAGTCT TATGCCTGGA TATCGGCATG TTTATCTGGA TGGTCTAACA 
GAAGCCTCCA TATTTGTTCA TGTTACAATT AATGAAATAT ATGGAAAGAA CAGGCAGTTT 
ATTGGCCTGA AAGGGTTTTT CAATAGGAGT TCTAAGCATA GCTCTGTTGA GAGCAATAGT 
CATGTTCGTA AGAAATCAAT TGGGGACCGT ATCTTGCGAC GCAGTGCGAG TGCTCCAGCC 
AAAGCCAGGA AGAAGACCAA GATGGTGTTT CAAGAGTCAA CTGAGAGTAA AGACATTGTC 
TGTGAAACAT CTGGTGCTAA AGAGAGAGAT TTTATGAGAA AGAGAACCTC AAGGAGTCTG 
CAAGCACGCC CTGTTTCAAT GCCTGTGGAT AAGTTACTAC TTGGGGCTTT TGAACCTTCA 
ACATCAGAGA TTTCTAAGGA GACTAGAAGT GACATAGCTT CAGGTTCAGC AGGGGAGATG 
CAAGCTAAGC ACCAGGAGAA GCCAGAGATG ACAAAACTGG ATCTACCAGT TCATAAAAAT 
AATCTTGCTA TTCAACATTC TTCCCCTCTT TTTAAAGATT GTCACCAGAC TGAAAATCCA 
GTAAATAAAC CTGTACATAA AAAATGGAGG AGCTTACATT TAGAAAAACA AAACTATGAT 
GATATTAGAG AGTTAAATGA TACATTTACC AGTGAATCTG ATGAGGAGAT TTTAAAGCCA 
AAAGAAGGTA ATGAAAAAGA AGCTATGCTG TTATTAGACA AAATGGAGTC CATCCCAGAA 
AACACCAAGC ATCATGCAAA AGCTGATTTA TTTTCTGTGA CTGATTTATC AAGCACTATA 
TGTACAGACA TGAATCACTT ACAGTCTACT GCACCTTTAT TAGAAACTGA TATTTCTCAT 
ATTATAAGTG AAGTTCCTTC AGTCAACGAG AGTGAACTTG GAAGCACGGT GTCTGCTTTA 
ATAAGGCACT TTGAGATCAC CGAAGACAAA ACTAATTTGA CATTTGTCTC AAGTTTGCAA 
GGTCCAAACA GCCAAAGCAA TTTTTGTGAC AATTCTGATG TGTTAATATC TGACCTTTTA 
AATAGAAAAA AGCACACAAG ACAATCTTTA CATTCAGCTT CCAACACTAC TGTGTTCTCC 
AGCCCTGAAA CAAGTGAATA TTCTATATAT ACATTTATTC ATGAAGAGCA TGTACCTCCT 
GATGCCAATT ACAAATTGAT GGATGCAAAC AATGGCATAT CATCGTGTTC TAATTTAAAA 
AATAATTCAT CAAACAGTTG GCTAGATAGT TCTGAGAGCC TACAGTTTTG TTCTCAATAT 
AATGCAAACA GCAATACATT AGGTCTAAAA TCAAAGCAAA GTAGGCCACA AGTTTCACTG 
AAAACATCAT CAGGCTGTAC AGATGAGAAT GTCAAACAGA TATCATCTCA CAACTCCTTT 
CTAAAGAACC TTAATGTAGA TAGTAATCCT AAGAGAATAG CGGATAAGAA TTTCTTACAT 
ACTGGTGTCA ATAGGTATGC CACACAAGCC TGTCCAAACT CTCAAAACAC AGGCATTCAA 
CACGGATCTG GTCAACTTTA TCAAAACAGG CATTCAAGTT ATAAACCAAG AGCAGATGCA 
CCACGAGACA AACAGTATAT TGCCCAGTCT GGAAACCTGC ACAGCTCGCC CAATGCAAAC 
TGGCCCAAAC CTTCAAACTA TCACATTCAT TCATTACAAG GTATTAAACA GCCAAGTCCA 
TGTATATCAA AAAGTCTTGG AGATCTGACA TCAGACGATA TATCTTGTAA TTTTGAAAGT 
AAGTACAAAC GGATCAACAG AGGGTTTCTT TCATCAGCTA TTTGTCCGGA AAATACATCA 
TTAGCTCAAA GCAGACCTCA GTCTACCGAT GCTTTAACGG AACAGTTGCG CAAACTGATG 
TCTTTTGACC AAGACGACAG TAACAGGCCA TCATATTATG AAGAAGAAGA ATGGGACAGT 
CCTAAGCCAC TTACAAGAAA GCTATCCTCA AGGAGTCAAA GTAGGGTCAG AAACATTGCT 
AACCGTGCCA AAGAAAGACA GGAGGCCAAT AAAATAAAAC AAAGCAATTT AAACTCAAGT 
GAAGTAGTCT TAAGAAACAA ACGTTCCATG GCTTCTCAGC CCATCAACAG GCATTCCACT 
GGTTCTTATA TAGGTAGCTT GTATAATAAC TTTAACAGTG AAAGGCTAGA AGGCAGAGGT 
ATACCAGAAG GTGCATGCAC TTCCCATACA CAAGTGTATA ATGACCACAT TGACACTGGC 
TTTACTTATC GACATGAGCC AAGCAATACT GATGAACCAG AAATTTATTT CCTACTAAGA 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plch1 ( XM_012963444.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818898.1 Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X1, mRNA.

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CloneID	OXa36823
Clone ID Related Accession (Same CDS sequence)	XM_012963444.2 , XM_012963444.3
Accession Version	XM_012963444.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963444.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCC AAAATCAGGA TAAGAGAAGA TTTCTGCAGT ACTGCCGCCA TTTCTTAGCT 
GACAATGGGG CATTTCATGT TGAAAGATGT ATGAGTGTAA TGCAGTCTGG GACACAGATG 
GTAAAACTAA AGAGTGGAAC AAAGGCTTTG GTTCGCCTCT TTTACCTGGA TGAACATCGG 
ACATGTATCA GATGGAGACC TTCAAGAAAA AGCGAGAAAG CTAAAATTAT AATTGATTCA 
ATTTACAAAG TCACGGAAGG GCGACAGTCT GAAATATTCC ACCGCCATGC AGAAGGAAAC 
TTTGATCCCA GTTGCTGTTT TACCATATAT CATGGAAACC ACATGGAATC TCTGGACCTC 
ATAACATCAA ATCCGGAGGA GGCCCGAACT TGGATCACAG GGCTGAAATA TCTGATGGCT 
GGAATTAGCG ATGAGGATTC TCTGGCAAAA AGGCAAAGGA CCCATGATCA ATGGGTGAAG 
CAGACATTTG AAGAGGCTGA TAAAAATGGG GATGGCTTAC TCAACATTGA GGAAATATAC 
CAGTTAATGC ATAAACTCAA CGTGAATCTG CCACGAAGGA AAGTTCGCCA GATGTTCCAG 
GAAGCAGACA CGGACGAGAA CCAAGGGACT CTGACCTTTG AAGAGTTTTC TGTGTTTTAC 
AAGATGATGT CATTACGGAG GGATTTGTAC CTGTTACTTT TAAACTACAG CGACAAAAAG 
GATCACTTAA CAGTTGACGA TCTGGCTCAG TTTTTCAAAG TAGAACAAAA GATGAATAAT 
GTGACAACAG AATACTGTAT TGACATTATA CAGAAATTTG AAGTCTCAGA GGAAAACAAA 
GAACAACATG TTCTGGGAAT AGATGGTTTT ACTAATTTCA TGAGAAGTCC TGCAGGAGAT 
ATTTTTAACC CTCAGCACTG TGAGATCTAC CAAGACATGG ACCAACCTCT CTGTAGCTAC 
TACATTGCAT CATCCCATAA CACCTACTTA ACTGGAGACC AACTTCTTTC TCAATCCAAA 
GTGGATATGT ATGCTTGGGT ATTGCAAGAT GGCTGTCGAT GCATAGAAGT TGACTGCTGG 
GATGGACCAG ATGGGGAGCC AGTGGTACAC CATGGCTATA CCCTTACTTC CAAAGTCCTT 
TTTAAAGATG TTGTGGAAAC CATCAACAAA CATGCTTTTG TGAAAAATGA ATATCCTGTT 
ATACTTTCAA TTGAAAACCA CTGCAACATT CAACAACAAA GAAAGATTGC TCAGTACATG 
AAAGATATAT TTGGGGATAA ACTTGATCTG TCTTCTGTCC CACTTGGAGA ATCCAAGCAA 
CTTCCTAGTC CTCAGAATCT CAAGGGAAAG ATATTAGTGA AGGGGAAAAA GTTGCCATAT 
GACCTGGCAG ACGATGCTGA GGAGGGAGAA GTCTCAGATG AAGATAGTGC TGACGAAATA 
GAAGAAGAAT GCAAACTCAC ACAATGTTTT AGTAATGGCA CAACAGAGCA CCAGGTGGAA 
TCTTTCATTC GGAAAAAACT GGAGTCTTTG CTAAAGGAAT CCCAGATCAG GGATAAGGAA 
GATCCAGATA GCTTTACCGT GAAAGCATTA CTAAAGGCAA CACATGAAGG CCTGAATGTT 
AATCTTAAAC AGAATGATGA CACAAAAGAT TGTGGAAAGT CTAAAAGGTC TTCAGTTGGA 
AGTATTGCCA AACACAAAAA AGCTTCCAAA TCCAGGTCTA AATCTTGCAG TACTAGTGAT 
GATGAAGATT CATCGCCAAA AGAGACAACA GCACAGGCAC ACAGGTTGGG TCGTCGAAGG 
AAGACAATGA AGTTATGTCG AGCTTTGTCA GACTTGGTTG TCTACACAAA TTCTGTGGCT 
GCACAAGACA TTGTAGATGA TGGGACCTCA GGAAACGTTT TATCCTTTAG TGAAACAAGA 
GCACACCAGG TGGTTCAGCA CAAATCTGAG CAGCTCATGT TGTACAATCA AAAACAATTA 
ACTAGGATTT ATCCATCTGC ATACAGGATA GACTCTAGTA ATTTTAACCC AGTGGCCTAC 
TGGAATGCAG GAATTCAGCT TGTTGCGTTA AACTATCAAA CAGAAGGACG CATGATGGAA 
TTAAATCGAG CTAAATTCAA GATGAATGGT GGTTGTGGAT ATGTCCTTAA GCCCCAGCAG 
ATGTGCAAAG GTGTATTTAA CCAATTTTCA GCTGACCCTC TTCCTGCCAG CCCAAAAAAG 
CAGTTAATTT TGAAGATAAT CAGTGGACAG CAACTTCCCA AACCACCAGA TTCAATGCTT 
GGGGATAGAG GAGAGATCAT TGACCCTTTT GTAGAAGTGG AAATTATCGG CTTGCCTGTG 
GACTGCTGTA AGGAACAGAC TCGAGTAGTT GATGACAATG GTTTCAATCC TGTATGGGAG 
GAAACTCTTA CCTTTACAGT TCATATGCCA GAAATAGCAT TGGTAAGGTT CTTAGTATGG 
GACCATGATC CAATTGGCCG GGATTTTGTT GGACAAAGGA CTGTAGCCTT TAACAGTCTT 
ATGCCTGGAT ATCGGCATGT TTATCTGGAT GGTCTAACAG AAGCCTCCAT ATTTGTTCAT 
GTTACAATTA ATGAAATATA TGGAAAGTGG AGTCCTTTAA TCCTCAATCC CAGTTACACA 
ATATTGCACT TTCTAGGAGC TACGAAGAAC AGGCAGTTTA TTGGCCTGAA AGGGTTTTTC 
AATAGGAGTT CTAAGCATAG CTCTGTTGAG AGCAATAGTC ATGTTCGTAA GAAATCAATT 
GGGGACCGTA TCTTGCGACG CAGTGCGAGT GCTCCAGCCA AAGCCAGGAA GAAGACCAAG 
ATGGTGTTTC AAGAGTCAAC TGAGAGTAAA GACATTGTCT GTGAAACATC TGGTGCTAAA 
GAGAGAGATT TTATGAGAAA GAGAACCTCA AGGAGTCTGC AAGCACGCCC TGTTTCAATG 
CCTGTGGATA AGTTACTACT TGGGGCTTTT GAACCTTCAA CATCAGAGAT TTCTAAGGAG 
ACTAGAAGTG ACATAGCTTC AGGTTCAGCA GGGGAGATGC AAGCTAAGCA CCAGGAGAAG 
CCAGAGATGA CAAAACTGGA TCTACCAGTT CATAAAAATA ATCTTGCTAT TCAACATTCT 
TCCCCTCTTT TTAAAGATTG TCACCAGACT GAAAATCCAG TAAATAAACC TGTACATAAA 
AAATGGAGGA GCTTACATTT AGAAAAACAA AACTATGATG ATATTAGAGA GTTAAATGAT 
ACATTTACCA GTGAATCTGA TGAGGAGATT TTAAAGCCAA AAGAAGGTAA TGAAAAAGAA 
GCTATGCTGT TATTAGACAA AATGGAGTCC ATCCCAGAAA ACACCAAGCA TCATGCAAAA 
GCTGATTTAT TTTCTGTGAC TGATTTATCA AGCACTATAT GTACAGACAT GAATCACTTA 
CAGTCTACTG CACCTTTATT AGAAACTGAT ATTTCTCATA TTATAAGTGA AGTTCCTTCA 
GTCAACGAGA GTGAACTTGG AAGCACGGTG TCTGCTTTAA TAAGGCACTT TGAGATCACC 
GAAGACAAAA CTAATTTGAC ATTTGTCTCA AGTTTGCAAG GTCCAAACAG CCAAAGCAAT 
TTTTGTGACA ATTCTGATGT GTTAATATCT GACCTTTTAA ATAGAAAAAA GCACACAAGA 
CAATCTTTAC ATTCAGCTTC CAACACTACT GTGTTCTCCA GCCCTGAAAC AAGTGAATAT 
TCTATATATA CATTTATTCA TGAAGAGCAT GTACCTCCTG ATGCCAATTA CAAATTGATG 
GATGCAAACA ATGGCATATC ATCGTGTTCT AATTTAAAAA ATAATTCATC AAACAGTTGG 
CTAGATAGTT CTGAGAGCCT ACAGTTTTGT TCTCAATATA ATGCAAACAG CAATACATTA 
GGTCTAAAAT CAAAGCAAAG TAGGCCACAA GTTTCACTGA AAACATCATC AGGCTGTACA 
GATGAGAATG TCAAACAGAT ATCATCTCAC AACTCCTTTC TAAAGAACCT TAATGTAGAT 
AGTAATCCTA AGAGAATAGC GGATAAGAAT TTCTTACATA CTGGTGTCAA TAGGTATGCC 
ACACAAGCCT GTCCAAACTC TCAAAACACA GGCATTCAAC ACGGATCTGG TCAACTTTAT 
CAAAACAGGC ATTCAAGTTA TAAACCAAGA GCAGATGCAC CACGAGACAA ACAGTATATT 
GCCCAGTCTG GAAACCTGCA CAGCTCGCCC AATGCAAACT GGCCCAAACC TTCAAACTAT 
CACATTCATT CATTACAAGG TATTAAACAG CCAAGTCCAT GTATATCAAA AAGTCTTGGA 
GATCTGACAT CAGACGATAT ATCTTGTAAT TTTGAAAGTA AGTACAAACG GATCAACAGA 
GGGTTTCTTT CATCAGCTAT TTGTCCGGAA AATACATCAT TAGCTCAAAG CAGACCTCAG 
TCTACCGATG CTTTAACGGA ACAGTTGCGC AAACTGATGT CTTTTGACCA AGACGACAGT 
AACAGGCCAT CATATTATGA AGAAGAAGAA TGGGACAGTC CTAAGCCACT TACAAGAAAG 
CTATCCTCAA GGAGTCAAAG TAGGGTCAGA AACATTGCTA ACCGTGCCAA AGAAAGACAG 
GAGGCCAATA AAATAAAACA AAGCAATTTA AACTCAAGTG AAGTAGTCTT AAGAAACAAA 
CGTTCCATGG CTTCTCAGCC CATCAACAGG CATTCCACTG GTTCTTATAT AGGTAGCTTG 
TATAATAACT TTAACAGTGA AAGGCTAGAA GGCAGAGGTA TACCAGAAGG TGCATGCACT 
TCCCATACAC AAGTGTATAA TGACCACATT GACACTGGCT TTACTTATCG ACATGAGCCA 
AGCAATACTG ATGAACCAGA AATTTATTTC CTACTAAGAC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818898.1
CDS	282..5186
Translation	MSSQNQDKRRFLQYCRHFLADNGAFHVERCMSVMQSGTQMVKLKSGTKALVRLFYLDEHRTCIRWRPSRKSEKAKIIIDSIYKVTEGRQSEIFHRHAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIYQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFSVFYKMMSLRRDLYLLLLNYSDKKDHLTVDDLAQFFKVEQKMNNVTTEYCIDIIQKFEVSEENKEQHVLGIDGFTNFMRSPAGDIFNPQHCEIYQDMDQPLCSYYIASSHNTYLTGDQLLSQSKVDMYAWVLQDGCRCIEVDCWDGPDGEPVVHHGYTLTSKVLFKDVVETINKHAFVKNEYPVILSIENHCNIQQQRKIAQYMKDIFGDKLDLSSVPLGESKQLPSPQNLKGKILVKGKKLPYDLADDAEEGEVSDEDSADEIEEECKLTQCFSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVKALLKATHEGLNVNLKQNDDTKDCGKSKRSSVGSIAKHKKASKSRSKSCSTSDDEDSSPKETTAQAHRLGRRRKTMKLCRALSDLVVYTNSVAAQDIVDDGTSGNVLSFSETRAHQVVQHKSEQLMLYNQKQLTRIYPSAYRIDSSNFNPVAYWNAGIQLVALNYQTEGRMMELNRAKFKMNGGCGYVLKPQQMCKGVFNQFSADPLPASPKKQLILKIISGQQLPKPPDSMLGDRGEIIDPFVEVEIIGLPVDCCKEQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVAFNSLMPGYRHVYLDGLTEASIFVHVTINEIYGKWSPLILNPSYTILHFLGATKNRQFIGLKGFFNRSSKHSSVESNSHVRKKSIGDRILRRSASAPAKARKKTKMVFQESTESKDIVCETSGAKERDFMRKRTSRSLQARPVSMPVDKLLLGAFEPSTSEISKETRSDIASGSAGEMQAKHQEKPEMTKLDLPVHKNNLAIQHSSPLFKDCHQTENPVNKPVHKKWRSLHLEKQNYDDIRELNDTFTSESDEEILKPKEGNEKEAMLLLDKMESIPENTKHHAKADLFSVTDLSSTICTDMNHLQSTAPLLETDISHIISEVPSVNESELGSTVSALIRHFEITEDKTNLTFVSSLQGPNSQSNFCDNSDVLISDLLNRKKHTRQSLHSASNTTVFSSPETSEYSIYTFIHEEHVPPDANYKLMDANNGISSCSNLKNNSSNSWLDSSESLQFCSQYNANSNTLGLKSKQSRPQVSLKTSSGCTDENVKQISSHNSFLKNLNVDSNPKRIADKNFLHTGVNRYATQACPNSQNTGIQHGSGQLYQNRHSSYKPRADAPRDKQYIAQSGNLHSSPNANWPKPSNYHIHSLQGIKQPSPCISKSLGDLTSDDISCNFESKYKRINRGFLSSAICPENTSLAQSRPQSTDALTEQLRKLMSFDQDDSNRPSYYEEEEWDSPKPLTRKLSSRSQSRVRNIANRAKERQEANKIKQSNLNSSEVVLRNKRSMASQPINRHSTGSYIGSLYNNFNSERLEGRGIPEGACTSHTQVYNDHIDTGFTYRHEPSNTDEPEIYFLLRL

Target ORF information:

RefSeq Version	XM_012963444.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C eta 1 (plch1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963444.2

ORF Insert Sequence:

ATGAGTTCCC AAAATCAGGA TAAGAGAAGA TTTCTGCAGT ACTGCCGCCA TTTCTTAGCT 
GACAATGGGG CATTTCATGT TGAAAGATGT ATGAGTGTAA TGCAGTCTGG GACACAGATG 
GTAAAACTAA AGAGTGGAAC AAAGGCTTTG GTTCGCCTCT TTTACCTGGA TGAACATCGG 
ACATGTATCA GATGGAGACC TTCAAGAAAA AGCGAGAAAG CTAAAATTAT AATTGATTCA 
ATTTACAAAG TCACGGAAGG GCGACAGTCT GAAATATTCC ACCGCCATGC AGAAGGAAAC 
TTTGATCCCA GTTGCTGTTT TACCATATAT CATGGAAACC ACATGGAATC TCTGGACCTC 
ATAACATCAA ATCCGGAGGA GGCCCGAACT TGGATCACAG GGCTGAAATA TCTGATGGCT 
GGAATTAGCG ATGAGGATTC TCTGGCAAAA AGGCAAAGGA CCCATGATCA ATGGGTGAAG 
CAGACATTTG AAGAGGCTGA TAAAAATGGG GATGGCTTAC TCAACATTGA GGAAATATAC 
CAGTTAATGC ATAAACTCAA CGTGAATCTG CCACGAAGGA AAGTTCGCCA GATGTTCCAG 
GAAGCAGACA CGGACGAGAA CCAAGGGACT CTGACCTTTG AAGAGTTTTC TGTGTTTTAC 
AAGATGATGT CATTACGGAG GGATTTGTAC CTGTTACTTT TAAACTACAG CGACAAAAAG 
GATCACTTAA CAGTTGACGA TCTGGCTCAG TTTTTCAAAG TAGAACAAAA GATGAATAAT 
GTGACAACAG AATACTGTAT TGACATTATA CAGAAATTTG AAGTCTCAGA GGAAAACAAA 
GAACAACATG TTCTGGGAAT AGATGGTTTT ACTAATTTCA TGAGAAGTCC TGCAGGAGAT 
ATTTTTAACC CTCAGCACTG TGAGATCTAC CAAGACATGG ACCAACCTCT CTGTAGCTAC 
TACATTGCAT CATCCCATAA CACCTACTTA ACTGGAGACC AACTTCTTTC TCAATCCAAA 
GTGGATATGT ATGCTTGGGT ATTGCAAGAT GGCTGTCGAT GCATAGAAGT TGACTGCTGG 
GATGGACCAG ATGGGGAGCC AGTGGTACAC CATGGCTATA CCCTTACTTC CAAAGTCCTT 
TTTAAAGATG TTGTGGAAAC CATCAACAAA CATGCTTTTG TGAAAAATGA ATATCCTGTT 
ATACTTTCAA TTGAAAACCA CTGCAACATT CAACAACAAA GAAAGATTGC TCAGTACATG 
AAAGATATAT TTGGGGATAA ACTTGATCTG TCTTCTGTCC CACTTGGAGA ATCCAAGCAA 
CTTCCTAGTC CTCAGAATCT CAAGGGAAAG ATATTAGTGA AGGGGAAAAA GTTGCCATAT 
GACCTGGCAG ACGATGCTGA GGAGGGAGAA GTCTCAGATG AAGATAGTGC TGACGAAATA 
GAAGAAGAAT GCAAACTCAC ACAATGTTTT AGTAATGGCA CAACAGAGCA CCAGGTGGAA 
TCTTTCATTC GGAAAAAACT GGAGTCTTTG CTAAAGGAAT CCCAGATCAG GGATAAGGAA 
GATCCAGATA GCTTTACCGT GAAAGCATTA CTAAAGGCAA CACATGAAGG CCTGAATGTT 
AATCTTAAAC AGAATGATGA CACAAAAGAT TGTGGAAAGT CTAAAAGGTC TTCAGTTGGA 
AGTATTGCCA AACACAAAAA AGCTTCCAAA TCCAGGTCTA AATCTTGCAG TACTAGTGAT 
GATGAAGATT CATCGCCAAA AGAGACAACA GCACAGGCAC ACAGGTTGGG TCGTCGAAGG 
AAGACAATGA AGTTATGTCG AGCTTTGTCA GACTTGGTTG TCTACACAAA TTCTGTGGCT 
GCACAAGACA TTGTAGATGA TGGGACCTCA GGAAACGTTT TATCCTTTAG TGAAACAAGA 
GCACACCAGG TGGTTCAGCA CAAATCTGAG CAGCTCATGT TGTACAATCA AAAACAATTA 
ACTAGGATTT ATCCATCTGC ATACAGGATA GACTCTAGTA ATTTTAACCC AGTGGCCTAC 
TGGAATGCAG GAATTCAGCT TGTTGCGTTA AACTATCAAA CAGAAGGACG CATGATGGAA 
TTAAATCGAG CTAAATTCAA GATGAATGGT GGTTGTGGAT ATGTCCTTAA GCCCCAGCAG 
ATGTGCAAAG GTGTATTTAA CCAATTTTCA GCTGACCCTC TTCCTGCCAG CCCAAAAAAG 
CAGTTAATTT TGAAGATAAT CAGTGGACAG CAACTTCCCA AACCACCAGA TTCAATGCTT 
GGGGATAGAG GAGAGATCAT TGACCCTTTT GTAGAAGTGG AAATTATCGG CTTGCCTGTG 
GACTGCTGTA AGGAACAGAC TCGAGTAGTT GATGACAATG GTTTCAATCC TGTATGGGAG 
GAAACTCTTA CCTTTACAGT TCATATGCCA GAAATAGCAT TGGTAAGGTT CTTAGTATGG 
GACCATGATC CAATTGGCCG GGATTTTGTT GGACAAAGGA CTGTAGCCTT TAACAGTCTT 
ATGCCTGGAT ATCGGCATGT TTATCTGGAT GGTCTAACAG AAGCCTCCAT ATTTGTTCAT 
GTTACAATTA ATGAAATATA TGGAAAGTGG AGTCCTTTAA TCCTCAATCC CAGTTACACA 
ATATTGCACT TTCTAGGAGC TACGAAGAAC AGGCAGTTTA TTGGCCTGAA AGGGTTTTTC 
AATAGGAGTT CTAAGCATAG CTCTGTTGAG AGCAATAGTC ATGTTCGTAA GAAATCAATT 
GGGGACCGTA TCTTGCGACG CAGTGCGAGT GCTCCAGCCA AAGCCAGGAA GAAGACCAAG 
ATGGTGTTTC AAGAGTCAAC TGAGAGTAAA GACATTGTCT GTGAAACATC TGGTGCTAAA 
GAGAGAGATT TTATGAGAAA GAGAACCTCA AGGAGTCTGC AAGCACGCCC TGTTTCAATG 
CCTGTGGATA AGTTACTACT TGGGGCTTTT GAACCTTCAA CATCAGAGAT TTCTAAGGAG 
ACTAGAAGTG ACATAGCTTC AGGTTCAGCA GGGGAGATGC AAGCTAAGCA CCAGGAGAAG 
CCAGAGATGA CAAAACTGGA TCTACCAGTT CATAAAAATA ATCTTGCTAT TCAACATTCT 
TCCCCTCTTT TTAAAGATTG TCACCAGACT GAAAATCCAG TAAATAAACC TGTACATAAA 
AAATGGAGGA GCTTACATTT AGAAAAACAA AACTATGATG ATATTAGAGA GTTAAATGAT 
ACATTTACCA GTGAATCTGA TGAGGAGATT TTAAAGCCAA AAGAAGGTAA TGAAAAAGAA 
GCTATGCTGT TATTAGACAA AATGGAGTCC ATCCCAGAAA ACACCAAGCA TCATGCAAAA 
GCTGATTTAT TTTCTGTGAC TGATTTATCA AGCACTATAT GTACAGACAT GAATCACTTA 
CAGTCTACTG CACCTTTATT AGAAACTGAT ATTTCTCATA TTATAAGTGA AGTTCCTTCA 
GTCAACGAGA GTGAACTTGG AAGCACGGTG TCTGCTTTAA TAAGGCACTT TGAGATCACC 
GAAGACAAAA CTAATTTGAC ATTTGTCTCA AGTTTGCAAG GTCCAAACAG CCAAAGCAAT 
TTTTGTGACA ATTCTGATGT GTTAATATCT GACCTTTTAA ATAGAAAAAA GCACACAAGA 
CAATCTTTAC ATTCAGCTTC CAACACTACT GTGTTCTCCA GCCCTGAAAC AAGTGAATAT 
TCTATATATA CATTTATTCA TGAAGAGCAT GTACCTCCTG ATGCCAATTA CAAATTGATG 
GATGCAAACA ATGGCATATC ATCGTGTTCT AATTTAAAAA ATAATTCATC AAACAGTTGG 
CTAGATAGTT CTGAGAGCCT ACAGTTTTGT TCTCAATATA ATGCAAACAG CAATACATTA 
GGTCTAAAAT CAAAGCAAAG TAGGCCACAA GTTTCACTGA AAACATCATC AGGCTGTACA 
GATGAGAATG TCAAACAGAT ATCATCTCAC AACTCCTTTC TAAAGAACCT TAATGTAGAT 
AGTAATCCTA AGAGAATAGC GGATAAGAAT TTCTTACATA CTGGTGTCAA TAGGTATGCC 
ACACAAGCCT GTCCAAACTC TCAAAACACA GGCATTCAAC ACGGATCTGG TCAACTTTAT 
CAAAACAGGC ATTCAAGTTA TAAACCAAGA GCAGATGCAC CACGAGACAA ACAGTATATT 
GCCCAGTCTG GAAACCTGCA CAGCTCGCCC AATGCAAACT GGCCCAAACC TTCAAACTAT 
CACATTCATT CATTACAAGG TATTAAACAG CCAAGTCCAT GTATATCAAA AAGTCTTGGA 
GATCTGACAT CAGACGATAT ATCTTGTAAT TTTGAAAGTA AGTACAAACG GATCAACAGA 
GGGTTTCTTT CATCAGCTAT TTGTCCGGAA AATACATCAT TAGCTCAAAG CAGACCTCAG 
TCTACCGATG CTTTAACGGA ACAGTTGCGC AAACTGATGT CTTTTGACCA AGACGACAGT 
AACAGGCCAT CATATTATGA AGAAGAAGAA TGGGACAGTC CTAAGCCACT TACAAGAAAG 
CTATCCTCAA GGAGTCAAAG TAGGGTCAGA AACATTGCTA ACCGTGCCAA AGAAAGACAG 
GAGGCCAATA AAATAAAACA AAGCAATTTA AACTCAAGTG AAGTAGTCTT AAGAAACAAA 
CGTTCCATGG CTTCTCAGCC CATCAACAGG CATTCCACTG GTTCTTATAT AGGTAGCTTG 
TATAATAACT TTAACAGTGA AAGGCTAGAA GGCAGAGGTA TACCAGAAGG TGCATGCACT 
TCCCATACAC AAGTGTATAA TGACCACATT GACACTGGCT TTACTTATCG ACATGAGCCA 
AGCAATACTG ATGAACCAGA AATTTATTTC CTACTAAGAC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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plch1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

オンラインフォーム

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