map3k4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	map3k4
Entrez Gene ID	100497217
Full Name	mitogen-activated protein kinase kinase kinase 4
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase 4 Names mitogen-activated protein kinase kinase kinase 4
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012963756.2	XP_012819210.1	mitogen-activated protein kinase kinase kinase 4 isoform X3
XM_004914648.4	XP_004914705.1	mitogen-activated protein kinase kinase kinase 4 isoform X2
XM_002936262.4	XP_002936308.2	mitogen-activated protein kinase kinase kinase 4 isoform X1
XM_004914648.3	XP_004914705.1	mitogen-activated protein kinase kinase kinase 4 isoform X2
XM_002936262.5	XP_002936308.2	mitogen-activated protein kinase kinase kinase 4 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Map3k4	NP_036078.2
Gallus gallus (chicken)	MAP3K4	XP_004935618.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002371	XP_312585.5
	MAP3K4	XP_001098969.2
Canis lupus familiaris (dog)	MAP3K4	XP_005615756.1
Danio rerio (zebrafish)	map3k4	XP_005157074.1
Xenopus tropicalis (tropical clawed frog)	map3k4	XP_004914705.1
Pan troglodytes (chimpanzee)	MAP3K4	XP_003311625.2
Bos taurus (cattle)	MAP3K4	XP_002690423.2
Rattus norvegicus (Norway rat)	Map3k4	NP_001100926.1
Drosophila melanogaster (fruit fly)	Mekk1	NP_650750.4
Homo sapiens (human)	MAP3K4	NP_005913.2

Gene Ontology

The following map3k4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the map3k4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa10659	XM_002936262.5 Latest version!	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa10660	XM_004914648.4 Latest version!	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa36814	XM_012963756.2 Latest version!	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OXa10660	XM_004914648.3	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa10659	XM_002936262.4	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

map3k4 ( XM_002936262.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936308.2 Xenopus tropicalis mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa10659
Clone ID Related Accession (Same CDS sequence)	XM_002936262.4 , XM_002936262.5
Accession Version	XM_002936262.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002936262.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAAA CTGAAGATGC TACTGATGAC AATCTGGATT GTGAAGCTGA TGAGTTTTCA 
GATGAAGCAA GTGCAGAATC TCTCTATAAC ACCTTTCCGA CCAGCATTTC CCGACAGATG 
AAGCGAATGT CATCCAAGAA CCAGTGGAAT AATACAGGGA GGCCAGTTGG CCATTTCACG 
TTCAATGAAA AGATGAGTAG ACCAAATCCA TCACCTCACA AAGAGAGCAG CAAAACCACT 
GGAAATGTTG AGGATCATAA CTATAAGCAA GAGAAGAAGA ACAGAGCCAC TTTAAGAATG 
ACTGAGCGGG ATCAAAAAAA AAATGTGCAG TGCTCCTTTT TGCTTGACCC AGGAAGTGGG 
CCCCTGACAA AAAAATCTAT TCCAGATGTT GATCTTAATA AACCATATCT CAGTCTTGGC 
TGCAGTAGTT CCAAGCTACC TGTATCTGTA CCTGTTTCAT TAGCTAGGAC TACAAGGCAG 
AGCTCCAGGA CTGACTGCCC AGCTGACCGT CTCAAGTTCT TTGAGACACT GCGACTTTTG 
TTGAAACTAA CTTCCGTCTC TAAGAAAAAG GAGAGAGAAC AAAGGGGTCA GGAAAATACT 
TCTTCTATGG GGTATAACCA GTCTAATGAA CTTTTCTGGC TGGAACTGCA AGCTTGGCAT 
GCTGGTCGTA GTATTAATGA CCAAGACTTG TACCTATACA CAGCTCGACA GACAATTCCT 
GACATCATTA ATGAAATTTT AACTTTTAAG GTGAACTATG GGTCTTTATT TAGCGATGCT 
GATAACATCA ATGGGACATT TAGAGAAAGA CCATTTATTG TCTCACCAGG ATCTTTTGCT 
TTTGGCCACT CAAGTTATCA TGAACATATC CAGAAGCAAA AAATATCGTT TGAACAAGTA 
AAGCAGATTA TGGACCTGCT GGAATACATT GAGGCACTTT ACCCATCTTT GAATGCTCTT 
CAGAGGGATT ATGAAAAATA TGCTGCAAAA GATTTTCAGG CTAGAGTGCA GGCACTTTGC 
CTGTGGCTTA ATATAACCAA GGACTTGAAT CACAAATTGA GGATAATGGG ATCTGTTCTT 
GGTATCAAGA ACTTGTCAAA CATTGGCTGG CCAGTGTTTG AAATTTCTTC ACCATGTCCA 
TCAAATTGCC CAGAGGATGA ACATGACAAC ATAGAACATG AGGGACAAAT ACATGATGGA 
GCACCTAGAA TGTGTAGAGA GGAAAGAGAA GAAGAAGAGC TAACAATTAA CAATTTAGAT 
TTGAGACAAG ATTTTAACAG CAAGCTAACA GTTAATTTTT TTGAAGAAGA CTACTCCTCC 
TCAAACAAAC TAGATAAGAT TATGTCAGAG GATGATTTTA GCTGGGGAAA TCCAGAGTGC 
AATGATAGTG ATTGTAATAG GCATTGTTCA ACCTCCATTT ATAGACCTTT TGTTGACAAA 
GCTCTGAAAC AGATGGGACT GAGGAAACTT ATTCTGAGAC TGCATAAATT AATGCATGGT 
TCTCTTCAGA GAGCACGTAG AGCCTTAATG ATGGCTGATC GTACATTTGA TTTTTCAGAA 
TACCCTGACC TGATGTTATG TTCGGATTAC TTGCCTGACC TGTTGAAGCA ATCACGTAGT 
ACCGTGCAGA GCTCAGAACC TGCTTGTGAC CCTGTGTCAT GGGAGGAATT AAAGTCGATG 
GACCTGCCAT CCTTTCTACC TGCTTTTTTA GTCCTCTGTC GGGTCCTTCT AAATGTTATT 
CATGAATGTC TCAAACTGAG ACTGGAGCAA AGGCCAGCTG GGGAACCATC ACTCCTGAGC 
ATTAAGCAGT TAGTGAGAGA GTGCAAAGAA GTTCTGAAGG GCGGACTTCT CATGAAACAA 
TATTATCAGT TCATGCTCCG AGAAGTGTTG GATGACCTGC AGAAATTGGA TTGTGACATT 
GATTCATTTG AAGATGATCT TCACAAAATG TTAATGGTGT ACTTTGACTA TATGCGGAGC 
TGGATTCAAA TGCTTCAACA GTTACCTCAA GCTTCACACA GTCTAAAAAA CCTGCTAGAG 
GAAGAGTGGA ATTTCACCAA AGAAATCACT CCACTTATAC GTGGGGGGGA AGCACAGGCA 
GGGAAGCTTT TTTGCGATAT TGCAGGAATG CTGCTGAAAT CCACTGGGAA ATTTCTTGAC 
TCTGGTCTTC TGGACAGCTG TGACGAATTC TGGGCCAGCA CTGATGATAG TAGCATCTCG 
GATGAAATAA GGAGATCTGT TATTGAAACC AGCCGAGCAC TGAAAGAGCT GTTTCATGAA 
GCCAGAGAGA GAGCATCCAA AGCACTTGGC TTTGCAAAGA TGCTGAGAAA GGATTTGGAA 
ATAGCTGCAG AATTTACTTT TGCCTCTACT ATTCTTGAAC TACTTGCATT TTTAAAAGCC 
AAGGATTATG CAAAGGTGCT CATCCCTTCT GTGGAAAACC TTCATGTGTT TATACCAGGT 
AATCTCTCTA GAGAAAAGAT CATTGTTCAG CAATTACTTG GTACTGCAGC TGGCATAGAC 
TGTTCCAAAG ACTGTGAGGA CATGGCATAT GACCAGTTCC TGCTCATGAC GAAGCATATC 
GATAAAGATA AACCTCAGGA GGATTGGAAC AGTTGGGATG GATGCTGTAT TAAAAGCATG 
CCACCAGTGG AAACAGCAGA TACTCTAAGA AGTATGCAGA CTGACAACCT TCTGCTGGTG 
GTAATGCAGT CAGCCCACCT CCAGAGTCAA AGAAAGGCTT TCCAGCACTC CACAGAAGGG 
CTTTTATCTT TGCGATTGGA GCAGACATCA AGCCATCCAA TGATTGCAGA GGCTTTGTTC 
CAACTCAAGA GTGAAGCTCT GCAACTCTGC AATGAAATTA GCAGTGCAAT TGACCGTGTT 
GATAAAATGT TCACCAATGA GTCTGAAGCA GAGATTGATG AATCAGAATC TTCTACTTTG 
CAGCAGTATT ACAGAGAAGC AATGATTCAA GGTTATAACT TTGGCTTTGA GTATCACAAA 
GAGGTGGTTC GTCTGATGTC TGGAGAGTTC AGACAGAAAA CTGGTGACAA ATATATCAAC 
TTTGCTAGAA AGTGGATGAA CTTTGTGCTG ACAAAATGTG AAAGTGGTCG CGGCACCAGA 
CCAAGGTGGG CAACCCAAGG TTTTGACTAT CTTCAAGCTA TTGAACCAGT ATTCATTTCA 
GCTCTCCCAG AGGATGACTT TTTGAGTCTA CAAGCTCTGA TGAATGAATG CATTGGCCAT 
GTTATTGGGA AACCCCACAG TCCTGTTACA GGTTTGTACA TTGCTACTCA TCGAAACAGC 
CCCCGTCCCG TGAAGGTGCC TCGATGCCAC AGTGACCCAC CAAATCCAAA TCTGATCACT 
CCAACTCCTG AAGGCTTCAG GGGTTCCAGT GTTCCCGAAA ATGATCGTTT GGCCTCCGTA 
GCTTCAGAGT TACAGTTCAG ATCCCTCAGT CGTCACTCTA GTCCAACAGA GGACAGGGAA 
GAGCCGTCCT ATCCAAAAGG GGAGTTGTGG GGGTCAACTC GGCGAAGCTG GGAGCTTCAG 
ACATTTATCA GTCAAACTCG AGACATAGCT GCAAGACACC GGCCTATGAA TGCTATACAG 
GAGTCGGTCA AACAGTTTGA GGATGAGCGC TACCGGCAAA TGAGAAATAA AAATATTGTC 
GGCCAAGTCT GTGATACCCC AAAATCCTAT GACAATGTAA TGCATGTTGG TCTTAGAAAA 
GTGACTTTCA AATGGCAGAG AGGAAACAAA ATTGGGGAAG GGCAGTATGG CAAAGTGTAC 
ACATGTATTA GCATTGACAC AGGGGAACTG ATGGCAATGA AAGAAATACG TTTTCAGCCA 
AATGATCACA AGACTATAAA GGAAACTGCA GATGAATTGA AAATTTTTGA AGGAATCAAA 
CACCCAAATC TTGTTCGTTA CTTTGGTGTC GAGCTCCACA GAGAAGAAAT GTATATTTTT 
ATGGAATACT GTGATGAAGG GACTTTGGAG GAGGTATCTA GACTGGGCCT GCAGGAGCAT 
GTCATTAGAC TTTATACAAA GCAGATAACC ATTGCTATAA ATGTCCTGCA TGAGCATGGT 
ATTGTTCATC GCGATATTAA AGGAGCAAAT ATATTTCTTA CATCTTCTGG ACTCATAAAA 
CTGGGCGATT TTGGATGTTC GGTCAAGCTG AAGAATAATG CCCAGACTAT GCCTGGAGAA 
GTTAACAGCA CACTTGGAAC TGCAGCATAT ATGGCTCCTG AAGTGATAAC CCAAGCTAAG 
GGAGAGGGGC ATGGGCGTGC TGCAGACATA TGGAGCCTTG GCTGTGTTTT AATTGAGATG 
GTTACTGGCA AGAGACCTTG GCATGAATAT GAACATAACT TCCAGATCAT GTACCGAGTC 
GGTATGGGAC ACAAGCCCCC CATTCCTGAT CGGCTGAGCC CAGAGGGTAA AGACTTCCTT 
TCTCATTGCC AGGAAAGTGA TCCAAAAATG CGATGGACAG CTAGCCAACT CTTGGACCAT 
GCATTTGTCA AGGTTTGCAC AGATGAAGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936308.2
CDS	93..4625
Translation	MKKTEDATDDNLDCEADEFSDEASAESLYNTFPTSISRQMKRMSSKNQWNNTGRPVGHFTFNEKMSRPNPSPHKESSKTTGNVEDHNYKQEKKNRATLRMTERDQKKNVQCSFLLDPGSGPLTKKSIPDVDLNKPYLSLGCSSSKLPVSVPVSLARTTRQSSRTDCPADRLKFFETLRLLLKLTSVSKKKEREQRGQENTSSMGYNQSNELFWLELQAWHAGRSINDQDLYLYTARQTIPDIINEILTFKVNYGSLFSDADNINGTFRERPFIVSPGSFAFGHSSYHEHIQKQKISFEQVKQIMDLLEYIEALYPSLNALQRDYEKYAAKDFQARVQALCLWLNITKDLNHKLRIMGSVLGIKNLSNIGWPVFEISSPCPSNCPEDEHDNIEHEGQIHDGAPRMCREEREEEELTINNLDLRQDFNSKLTVNFFEEDYSSSNKLDKIMSEDDFSWGNPECNDSDCNRHCSTSIYRPFVDKALKQMGLRKLILRLHKLMHGSLQRARRALMMADRTFDFSEYPDLMLCSDYLPDLLKQSRSTVQSSEPACDPVSWEELKSMDLPSFLPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLREVLDDLQKLDCDIDSFEDDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITPLIRGGEAQAGKLFCDIAGMLLKSTGKFLDSGLLDSCDEFWASTDDSSISDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFTFASTILELLAFLKAKDYAKVLIPSVENLHVFIPGNLSREKIIVQQLLGTAAGIDCSKDCEDMAYDQFLLMTKHIDKDKPQEDWNSWDGCCIKSMPPVETADTLRSMQTDNLLLVVMQSAHLQSQRKAFQHSTEGLLSLRLEQTSSHPMIAEALFQLKSEALQLCNEISSAIDRVDKMFTNESEAEIDESESSTLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKTGDKYINFARKWMNFVLTKCESGRGTRPRWATQGFDYLQAIEPVFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYIATHRNSPRPVKVPRCHSDPPNPNLITPTPEGFRGSSVPENDRLASVASELQFRSLSRHSSPTEDREEPSYPKGELWGSTRRSWELQTFISQTRDIAARHRPMNAIQESVKQFEDERYRQMRNKNIVGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISIDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYTKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITQAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYRVGMGHKPPIPDRLSPEGKDFLSHCQESDPKMRWTASQLLDHAFVKVCTDEE

Target ORF information:

RefSeq Version	XM_002936262.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936262.5

ORF Insert Sequence:

ATGAAAAAAA CTGAAGATGC TACTGATGAC AATCTGGATT GTGAAGCTGA TGAGTTTTCA 
GATGAAGCAA GTGCAGAATC TCTCTATAAC ACCTTTCCGA CCAGCATTTC CCGACAGATG 
AAGCGAATGT CATCCAAGAA CCAGTGGAAT AATACAGGGA GGCCAGTTGG CCATTTCACG 
TTCAATGAAA AGATGAGTAG ACCAAATCCA TCACCTCACA AAGAGAGCAG CAAAACCACT 
GGAAATGTTG AGGATCATAA CTATAAGCAA GAGAAGAAGA ACAGAGCCAC TTTAAGAATG 
ACTGAGCGGG ATCAAAAAAA AAATGTGCAG TGCTCCTTTT TGCTTGACCC AGGAAGTGGG 
CCCCTGACAA AAAAATCTAT TCCAGATGTT GATCTTAATA AACCATATCT CAGTCTTGGC 
TGCAGTAGTT CCAAGCTACC TGTATCTGTA CCTGTTTCAT TAGCTAGGAC TACAAGGCAG 
AGCTCCAGGA CTGACTGCCC AGCTGACCGT CTCAAGTTCT TTGAGACACT GCGACTTTTG 
TTGAAACTAA CTTCCGTCTC TAAGAAAAAG GAGAGAGAAC AAAGGGGTCA GGAAAATACT 
TCTTCTATGG GGTATAACCA GTCTAATGAA CTTTTCTGGC TGGAACTGCA AGCTTGGCAT 
GCTGGTCGTA GTATTAATGA CCAAGACTTG TACCTATACA CAGCTCGACA GACAATTCCT 
GACATCATTA ATGAAATTTT AACTTTTAAG GTGAACTATG GGTCTTTATT TAGCGATGCT 
GATAACATCA ATGGGACATT TAGAGAAAGA CCATTTATTG TCTCACCAGG ATCTTTTGCT 
TTTGGCCACT CAAGTTATCA TGAACATATC CAGAAGCAAA AAATATCGTT TGAACAAGTA 
AAGCAGATTA TGGACCTGCT GGAATACATT GAGGCACTTT ACCCATCTTT GAATGCTCTT 
CAGAGGGATT ATGAAAAATA TGCTGCAAAA GATTTTCAGG CTAGAGTGCA GGCACTTTGC 
CTGTGGCTTA ATATAACCAA GGACTTGAAT CACAAATTGA GGATAATGGG ATCTGTTCTT 
GGTATCAAGA ACTTGTCAAA CATTGGCTGG CCAGTGTTTG AAATTTCTTC ACCATGTCCA 
TCAAATTGCC CAGAGGATGA ACATGACAAC ATAGAACATG AGGGACAAAT ACATGATGGA 
GCACCTAGAA TGTGTAGAGA GGAAAGAGAA GAAGAAGAGC TAACAATTAA CAATTTAGAT 
TTGAGACAAG ATTTTAACAG CAAGCTAACA GTTAATTTTT TTGAAGAAGA CTACTCCTCC 
TCAAACAAAC TAGATAAGAT TATGTCAGAG GATGATTTTA GCTGGGGAAA TCCAGAGTGC 
AATGATAGTG ATTGTAATAG GCATTGTTCA ACCTCCATTT ATAGACCTTT TGTTGACAAA 
GCTCTGAAAC AGATGGGACT GAGGAAACTT ATTCTGAGAC TGCATAAATT AATGCATGGT 
TCTCTTCAGA GAGCACGTAG AGCCTTAATG ATGGCTGATC GTACATTTGA TTTTTCAGAA 
TACCCTGACC TGATGTTATG TTCGGATTAC TTGCCTGACC TGTTGAAGCA ATCACGTAGT 
ACCGTGCAGA GCTCAGAACC TGCTTGTGAC CCTGTGTCAT GGGAGGAATT AAAGTCGATG 
GACCTGCCAT CCTTTCTACC TGCTTTTTTA GTCCTCTGTC GGGTCCTTCT AAATGTTATT 
CATGAATGTC TCAAACTGAG ACTGGAGCAA AGGCCAGCTG GGGAACCATC ACTCCTGAGC 
ATTAAGCAGT TAGTGAGAGA GTGCAAAGAA GTTCTGAAGG GCGGACTTCT CATGAAACAA 
TATTATCAGT TCATGCTCCG AGAAGTGTTG GATGACCTGC AGAAATTGGA TTGTGACATT 
GATTCATTTG AAGATGATCT TCACAAAATG TTAATGGTGT ACTTTGACTA TATGCGGAGC 
TGGATTCAAA TGCTTCAACA GTTACCTCAA GCTTCACACA GTCTAAAAAA CCTGCTAGAG 
GAAGAGTGGA ATTTCACCAA AGAAATCACT CCACTTATAC GTGGGGGGGA AGCACAGGCA 
GGGAAGCTTT TTTGCGATAT TGCAGGAATG CTGCTGAAAT CCACTGGGAA ATTTCTTGAC 
TCTGGTCTTC TGGACAGCTG TGACGAATTC TGGGCCAGCA CTGATGATAG TAGCATCTCG 
GATGAAATAA GGAGATCTGT TATTGAAACC AGCCGAGCAC TGAAAGAGCT GTTTCATGAA 
GCCAGAGAGA GAGCATCCAA AGCACTTGGC TTTGCAAAGA TGCTGAGAAA GGATTTGGAA 
ATAGCTGCAG AATTTACTTT TGCCTCTACT ATTCTTGAAC TACTTGCATT TTTAAAAGCC 
AAGGATTATG CAAAGGTGCT CATCCCTTCT GTGGAAAACC TTCATGTGTT TATACCAGGT 
AATCTCTCTA GAGAAAAGAT CATTGTTCAG CAATTACTTG GTACTGCAGC TGGCATAGAC 
TGTTCCAAAG ACTGTGAGGA CATGGCATAT GACCAGTTCC TGCTCATGAC GAAGCATATC 
GATAAAGATA AACCTCAGGA GGATTGGAAC AGTTGGGATG GATGCTGTAT TAAAAGCATG 
CCACCAGTGG AAACAGCAGA TACTCTAAGA AGTATGCAGA CTGACAACCT TCTGCTGGTG 
GTAATGCAGT CAGCCCACCT CCAGAGTCAA AGAAAGGCTT TCCAGCACTC CACAGAAGGG 
CTTTTATCTT TGCGATTGGA GCAGACATCA AGCCATCCAA TGATTGCAGA GGCTTTGTTC 
CAACTCAAGA GTGAAGCTCT GCAACTCTGC AATGAAATTA GCAGTGCAAT TGACCGTGTT 
GATAAAATGT TCACCAATGA GTCTGAAGCA GAGATTGATG AATCAGAATC TTCTACTTTG 
CAGCAGTATT ACAGAGAAGC AATGATTCAA GGTTATAACT TTGGCTTTGA GTATCACAAA 
GAGGTGGTTC GTCTGATGTC TGGAGAGTTC AGACAGAAAA CTGGTGACAA ATATATCAAC 
TTTGCTAGAA AGTGGATGAA CTTTGTGCTG ACAAAATGTG AAAGTGGTCG CGGCACCAGA 
CCAAGGTGGG CAACCCAAGG TTTTGACTAT CTTCAAGCTA TTGAACCAGT ATTCATTTCA 
GCTCTCCCAG AGGATGACTT TTTGAGTCTA CAAGCTCTGA TGAATGAATG CATTGGCCAT 
GTTATTGGGA AACCCCACAG TCCTGTTACA GGTTTGTACA TTGCTACTCA TCGAAACAGC 
CCCCGTCCCG TGAAGGTGCC TCGATGCCAC AGTGACCCAC CAAATCCAAA TCTGATCACT 
CCAACTCCTG AAGGCTTCAG GGGTTCCAGT GTTCCCGAAA ATGATCGTTT GGCCTCCGTA 
GCTTCAGAGT TACAGTTCAG ATCCCTCAGT CGTCACTCTA GTCCAACAGA GGACAGGGAA 
GAGCCGTCCT ATCCAAAAGG GGAGTTGTGG GGGTCAACTC GGCGAAGCTG GGAGCTTCAG 
ACATTTATCA GTCAAACTCG AGACATAGCT GCAAGACACC GGCCTATGAA TGCTATACAG 
GAGTCGGTCA AACAGTTTGA GGATGAGCGC TACCGGCAAA TGAGAAATAA AAATATTGTC 
GGCCAAGTCT GTGATACCCC AAAATCCTAT GACAATGTAA TGCATGTTGG TCTTAGAAAA 
GTGACTTTCA AATGGCAGAG AGGAAACAAA ATTGGGGAAG GGCAGTATGG CAAAGTGTAC 
ACATGTATTA GCATTGACAC AGGGGAACTG ATGGCAATGA AAGAAATACG TTTTCAGCCA 
AATGATCACA AGACTATAAA GGAAACTGCA GATGAATTGA AAATTTTTGA AGGAATCAAA 
CACCCAAATC TTGTTCGTTA CTTTGGTGTC GAGCTCCACA GAGAAGAAAT GTATATTTTT 
ATGGAATACT GTGATGAAGG GACTTTGGAG GAGGTATCTA GACTGGGCCT GCAGGAGCAT 
GTCATTAGAC TTTATACAAA GCAGATAACC ATTGCTATAA ATGTCCTGCA TGAGCATGGT 
ATTGTTCATC GCGATATTAA AGGAGCAAAT ATATTTCTTA CATCTTCTGG ACTCATAAAA 
CTGGGCGATT TTGGATGTTC GGTCAAGCTG AAGAATAATG CCCAGACTAT GCCTGGAGAA 
GTTAACAGCA CACTTGGAAC TGCAGCATAT ATGGCTCCTG AAGTGATAAC CCAAGCTAAG 
GGAGAGGGGC ATGGGCGTGC TGCAGACATA TGGAGCCTTG GCTGTGTTTT AATTGAGATG 
GTTACTGGCA AGAGACCTTG GCATGAATAT GAACATAACT TCCAGATCAT GTACCGAGTC 
GGTATGGGAC ACAAGCCCCC CATTCCTGAT CGGCTGAGCC CAGAGGGTAA AGACTTCCTT 
TCTCATTGCC AGGAAAGTGA TCCAAAAATG CGATGGACAG CTAGCCAACT CTTGGACCAT 
GCATTTGTCA AGGTTTGCAC AGATGAAGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k4 ( XM_004914648.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914705.1 Xenopus tropicalis mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa10660
Clone ID Related Accession (Same CDS sequence)	XM_004914648.4 , XM_004914648.3
Accession Version	XM_004914648.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914648.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAAA CTGAAGATGC TACTGATGAC AATCTGGATT GTGAAGCTGA TGAGTTTTCA 
GATGAAGCAA GTGCAGAATC TCTCTATAAC ACCTTTCCGA CCAGCATTTC CCGACAGATG 
AAGCGAATGT CATCCAAGAA CCAGTGGAAT AATACAGGGA GGCCAGTTGG CCATTTCACG 
TTCAATGAAA AGATGAGTAG ACCAAATCCA TCACCTCACA AAGAGAGCAG CAAAACCACT 
GGAAATGTTG AGGATCATAA CTATAAGCAA GAGAAGAAGA ACAGAGCCAC TTTAAGAATG 
ACTGAGCGGG ATCAAAAAAA AAATGTGCAG TGCTCCTTTT TGCTTGACCC AGGAAGTGGG 
CCCCTGACAA AAAAATCTAT TCCAGATGTT GATCTTAATA AACCATATCT CAGTCTTGGC 
TGCAGTAGTT CCAAGCTACC TGTATCTGTA CCTGTTTCAT TAGCTAGGAC TACAAGGCAG 
AGCTCCAGGA CTGACTGCCC AGCTGACCGT CTCAAGTTCT TTGAGACACT GCGACTTTTG 
TTGAAACTAA CTTCCGTCTC TAAGAAAAAG GAGAGAGAAC AAAGGGGTCA GGAAAATACT 
TCTTCTATGG GGTATAACCA GTCTAATGAA CTTTTCTGGC TGGAACTGCA AGCTTGGCAT 
GCTGGTCGTA GTATTAATGA CCAAGACTTG TACCTATACA CAGCTCGACA GACAATTCCT 
GACATCATTA ATGAAATTTT AACTTTTAAG GTGAACTATG GGTCTTTATT TAGCGATGCT 
GATAACATCA ATGGGACATT TAGAGAAAGA CCATTTATTG TCTCACCAGG ATCTTTTGCT 
TTTGGCCACT CAAGTTATCA TGAACATATC CAGAAGCAAA AAATATCGTT TGAACAAGTA 
AAGCAGATTA TGGACCTGCT GGAATACATT GAGGCACTTT ACCCATCTTT GAATGCTCTT 
CAGAGGGATT ATGAAAAATA TGCTGCAAAA GATTTTCAGG CTAGAGTGCA GGCACTTTGC 
CTGTGGCTTA ATATAACCAA GGACTTGAAT CACAAATTGA GGATAATGGG ATCTGTTCTT 
GGTATCAAGA ACTTGTCAAA CATTGGCTGG CCAGTGTTTG AAATTTCTTC ACCATGTCCA 
TCAAATTGCC CAGAGGATGA ACATGACAAC ATAGAACATG AGGGACAAAT ACATGATGGA 
GCACCTAGAA TGTGTAGAGA GGAAAGAGAA GAAGAAGAGC TAACAATTAA CAATTTAGAT 
TTGAGACAAG ATTTTAACAG CAAGCTAACA GTTAATTTTT TTGAAGAAGA CTACTCCTCC 
TCAAACAAAC TAGATAAGAT TATGTCAGAG GATGATTTTA GCTGGGGAAA TCCAGAGTGC 
AATGATAGTG ATTGTAATAG GCATTGTTCA ACCTCCATTT ATAGACCTTT TGTTGACAAA 
GCTCTGAAAC AGATGGGACT GAGGAAACTT ATTCTGAGAC TGCATAAATT AATGCATGGT 
TCTCTTCAGA GAGCACGTAG AGCCTTAATG ATGGCTGATC GTACATTTGA TTTTTCAGAA 
TACCCTGACC TGATGTTATG TTCGGATTAC TTGCCTGACC TGTTGAAGCA ATCACGTAGT 
ACCGTGCAGA GCTCAGAACC TGCTTGTGAC CCTGTGTCAT GGGAGGAATT AAAGTCGATG 
GACCTGCCAT CCTTTCTACC TGCTTTTTTA GTCCTCTGTC GGGTCCTTCT AAATGTTATT 
CATGAATGTC TCAAACTGAG ACTGGAGCAA AGGCCAGCTG GGGAACCATC ACTCCTGAGC 
ATTAAGCAGT TAGTGAGAGA GTGCAAAGAA GTTCTGAAGG GCGGACTTCT CATGAAACAA 
TATTATCAGT TCATGCTCCG AGAAGTGTTG GATGACCTGC AGAAATTGGA TTGTGACATT 
GATTCATTTG AAGATGATCT TCACAAAATG TTAATGGTGT ACTTTGACTA TATGCGGAGC 
TGGATTCAAA TGCTTCAACA GTTACCTCAA GCTTCACACA GTCTAAAAAA CCTGCTAGAG 
GAAGAGTGGA ATTTCACCAA AGAAATCACT CCACTTATAC GTGGGGGGGA AGCACAGGCA 
GGGAAGCTTT TTTGCGATAT TGCAGGAATG CTGCTGAAAT CCACTGGGAA ATTTCTTGAC 
TCTGGTCTTC TGGACAGCTG TGACGAATTC TGGGCCAGCA CTGATGATAG TAGCATCTCG 
GATGAAATAA GGAGATCTGT TATTGAAACC AGCCGAGCAC TGAAAGAGCT GTTTCATGAA 
GCCAGAGAGA GAGCATCCAA AGCACTTGGC TTTGCAAAGA TGCTGAGAAA GGATTTGGAA 
ATAGCTGCAG AATTTACTTT TGCCTCTACT ATTCTTGAAC TACTTGCATT TTTAAAAGCC 
AAGGATTATG CAAAGGTGCT CATCCCTTCT GTGGAAAACC TTCATGTGTT TATACCAGGT 
AATCTCTCTA GAGAAAAGAT CATTGTTCAG CAATTACTTG GTACTGCAGC TGGCATAGAC 
TGTTCCAAAG ACTGTGAGGA CATGGCATAT GACCAGTTCC TGCTCATGAC GAAGCATATC 
GATAAAGATA AACCTCAGGA GGATTGGAAC AGTTGGGATG GATGCTGTAT TAAAAGCATG 
CCACCAGTGG AAACAGCAGA TACTCTAAGA AGTATGCAGA CTGACAACCT TCTGCTGGTG 
GTAATGCAGT CAGCCCACCT CCAGAGTCAA AGAAAGGCTT TCCAGCACTC CACAGAAGGG 
CTTTTATCTT TGCGATTGGA GCAGACATCA AGCCATCCAA TGATTGCAGA GGCTTTGTTC 
CAACTCAAGA GTGAAGCTCT GCAACTCTGC AATGAAATTA GCAGTGCAAT TGACCGTGTT 
GATAAAATGT TCACCAATGA GTCTGAAGCA GAGATTGATG AATCAGAATC TTCTACTTTG 
CAGCAGTATT ACAGAGAAGC AATGATTCAA GGTTATAACT TTGGCTTTGA GTATCACAAA 
GAGGTGGTTC GTCTGATGTC TGGAGAGTTC AGACAGAAAA CTGGTGACAA ATATATCAAC 
TTTGCTAGAA AGTGGATGAA CTTTGTGCTG ACAAAATGTG AAAGTGGTCG CGGCACCAGA 
CCAAGGTGGG CAACCCAAGG TTTTGACTAT CTTCAAGCTA TTGAACCAGT ATTCATTTCA 
GCTCTCCCAG AGGATGACTT TTTGAGTCTA CAAGCTCTGA TGAATGAATG CATTGGCCAT 
GTTATTGGGA AACCCCACAG TCCTGTTACA GCTACTCATC GAAACAGCCC CCGTCCCGTG 
AAGGTGCCTC GATGCCACAG TGACCCACCA AATCCAAATC TGATCACTCC AACTCCTGAA 
GGCTTCAGGG GTTCCAGTGT TCCCGAAAAT GATCGTTTGG CCTCCGTAGC TTCAGAGTTA 
CAGTTCAGAT CCCTCAGTCG TCACTCTAGT CCAACAGAGG ACAGGGAAGA GCCGTCCTAT 
CCAAAAGGGG AGTTGTGGGG GTCAACTCGG CGAAGCTGGG AGCTTCAGAC ATTTATCAGT 
CAAACTCGAG ACATAGCTGC AAGACACCGG CCTATGAATG CTATACAGGA GTCGGTCAAA 
CAGTTTGAGG ATGAGCGCTA CCGGCAAATG AGAAATAAAA ATATTGTCGG CCAAGTCTGT 
GATACCCCAA AATCCTATGA CAATGTAATG CATGTTGGTC TTAGAAAAGT GACTTTCAAA 
TGGCAGAGAG GAAACAAAAT TGGGGAAGGG CAGTATGGCA AAGTGTACAC ATGTATTAGC 
ATTGACACAG GGGAACTGAT GGCAATGAAA GAAATACGTT TTCAGCCAAA TGATCACAAG 
ACTATAAAGG AAACTGCAGA TGAATTGAAA ATTTTTGAAG GAATCAAACA CCCAAATCTT 
GTTCGTTACT TTGGTGTCGA GCTCCACAGA GAAGAAATGT ATATTTTTAT GGAATACTGT 
GATGAAGGGA CTTTGGAGGA GGTATCTAGA CTGGGCCTGC AGGAGCATGT CATTAGACTT 
TATACAAAGC AGATAACCAT TGCTATAAAT GTCCTGCATG AGCATGGTAT TGTTCATCGC 
GATATTAAAG GAGCAAATAT ATTTCTTACA TCTTCTGGAC TCATAAAACT GGGCGATTTT 
GGATGTTCGG TCAAGCTGAA GAATAATGCC CAGACTATGC CTGGAGAAGT TAACAGCACA 
CTTGGAACTG CAGCATATAT GGCTCCTGAA GTGATAACCC AAGCTAAGGG AGAGGGGCAT 
GGGCGTGCTG CAGACATATG GAGCCTTGGC TGTGTTTTAA TTGAGATGGT TACTGGCAAG 
AGACCTTGGC ATGAATATGA ACATAACTTC CAGATCATGT ACCGAGTCGG TATGGGACAC 
AAGCCCCCCA TTCCTGATCG GCTGAGCCCA GAGGGTAAAG ACTTCCTTTC TCATTGCCAG 
GAAAGTGATC CAAAAATGCG ATGGACAGCT AGCCAACTCT TGGACCATGC ATTTGTCAAG 
GTTTGCACAG ATGAAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914705.1
CDS	93..4613
Translation	MKKTEDATDDNLDCEADEFSDEASAESLYNTFPTSISRQMKRMSSKNQWNNTGRPVGHFTFNEKMSRPNPSPHKESSKTTGNVEDHNYKQEKKNRATLRMTERDQKKNVQCSFLLDPGSGPLTKKSIPDVDLNKPYLSLGCSSSKLPVSVPVSLARTTRQSSRTDCPADRLKFFETLRLLLKLTSVSKKKEREQRGQENTSSMGYNQSNELFWLELQAWHAGRSINDQDLYLYTARQTIPDIINEILTFKVNYGSLFSDADNINGTFRERPFIVSPGSFAFGHSSYHEHIQKQKISFEQVKQIMDLLEYIEALYPSLNALQRDYEKYAAKDFQARVQALCLWLNITKDLNHKLRIMGSVLGIKNLSNIGWPVFEISSPCPSNCPEDEHDNIEHEGQIHDGAPRMCREEREEEELTINNLDLRQDFNSKLTVNFFEEDYSSSNKLDKIMSEDDFSWGNPECNDSDCNRHCSTSIYRPFVDKALKQMGLRKLILRLHKLMHGSLQRARRALMMADRTFDFSEYPDLMLCSDYLPDLLKQSRSTVQSSEPACDPVSWEELKSMDLPSFLPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLREVLDDLQKLDCDIDSFEDDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITPLIRGGEAQAGKLFCDIAGMLLKSTGKFLDSGLLDSCDEFWASTDDSSISDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFTFASTILELLAFLKAKDYAKVLIPSVENLHVFIPGNLSREKIIVQQLLGTAAGIDCSKDCEDMAYDQFLLMTKHIDKDKPQEDWNSWDGCCIKSMPPVETADTLRSMQTDNLLLVVMQSAHLQSQRKAFQHSTEGLLSLRLEQTSSHPMIAEALFQLKSEALQLCNEISSAIDRVDKMFTNESEAEIDESESSTLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKTGDKYINFARKWMNFVLTKCESGRGTRPRWATQGFDYLQAIEPVFISALPEDDFLSLQALMNECIGHVIGKPHSPVTATHRNSPRPVKVPRCHSDPPNPNLITPTPEGFRGSSVPENDRLASVASELQFRSLSRHSSPTEDREEPSYPKGELWGSTRRSWELQTFISQTRDIAARHRPMNAIQESVKQFEDERYRQMRNKNIVGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISIDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYTKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITQAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYRVGMGHKPPIPDRLSPEGKDFLSHCQESDPKMRWTASQLLDHAFVKVCTDEE

Target ORF information:

RefSeq Version	XM_004914648.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914648.4

ORF Insert Sequence:

ATGAAAAAAA CTGAAGATGC TACTGATGAC AATCTGGATT GTGAAGCTGA TGAGTTTTCA 
GATGAAGCAA GTGCAGAATC TCTCTATAAC ACCTTTCCGA CCAGCATTTC CCGACAGATG 
AAGCGAATGT CATCCAAGAA CCAGTGGAAT AATACAGGGA GGCCAGTTGG CCATTTCACG 
TTCAATGAAA AGATGAGTAG ACCAAATCCA TCACCTCACA AAGAGAGCAG CAAAACCACT 
GGAAATGTTG AGGATCATAA CTATAAGCAA GAGAAGAAGA ACAGAGCCAC TTTAAGAATG 
ACTGAGCGGG ATCAAAAAAA AAATGTGCAG TGCTCCTTTT TGCTTGACCC AGGAAGTGGG 
CCCCTGACAA AAAAATCTAT TCCAGATGTT GATCTTAATA AACCATATCT CAGTCTTGGC 
TGCAGTAGTT CCAAGCTACC TGTATCTGTA CCTGTTTCAT TAGCTAGGAC TACAAGGCAG 
AGCTCCAGGA CTGACTGCCC AGCTGACCGT CTCAAGTTCT TTGAGACACT GCGACTTTTG 
TTGAAACTAA CTTCCGTCTC TAAGAAAAAG GAGAGAGAAC AAAGGGGTCA GGAAAATACT 
TCTTCTATGG GGTATAACCA GTCTAATGAA CTTTTCTGGC TGGAACTGCA AGCTTGGCAT 
GCTGGTCGTA GTATTAATGA CCAAGACTTG TACCTATACA CAGCTCGACA GACAATTCCT 
GACATCATTA ATGAAATTTT AACTTTTAAG GTGAACTATG GGTCTTTATT TAGCGATGCT 
GATAACATCA ATGGGACATT TAGAGAAAGA CCATTTATTG TCTCACCAGG ATCTTTTGCT 
TTTGGCCACT CAAGTTATCA TGAACATATC CAGAAGCAAA AAATATCGTT TGAACAAGTA 
AAGCAGATTA TGGACCTGCT GGAATACATT GAGGCACTTT ACCCATCTTT GAATGCTCTT 
CAGAGGGATT ATGAAAAATA TGCTGCAAAA GATTTTCAGG CTAGAGTGCA GGCACTTTGC 
CTGTGGCTTA ATATAACCAA GGACTTGAAT CACAAATTGA GGATAATGGG ATCTGTTCTT 
GGTATCAAGA ACTTGTCAAA CATTGGCTGG CCAGTGTTTG AAATTTCTTC ACCATGTCCA 
TCAAATTGCC CAGAGGATGA ACATGACAAC ATAGAACATG AGGGACAAAT ACATGATGGA 
GCACCTAGAA TGTGTAGAGA GGAAAGAGAA GAAGAAGAGC TAACAATTAA CAATTTAGAT 
TTGAGACAAG ATTTTAACAG CAAGCTAACA GTTAATTTTT TTGAAGAAGA CTACTCCTCC 
TCAAACAAAC TAGATAAGAT TATGTCAGAG GATGATTTTA GCTGGGGAAA TCCAGAGTGC 
AATGATAGTG ATTGTAATAG GCATTGTTCA ACCTCCATTT ATAGACCTTT TGTTGACAAA 
GCTCTGAAAC AGATGGGACT GAGGAAACTT ATTCTGAGAC TGCATAAATT AATGCATGGT 
TCTCTTCAGA GAGCACGTAG AGCCTTAATG ATGGCTGATC GTACATTTGA TTTTTCAGAA 
TACCCTGACC TGATGTTATG TTCGGATTAC TTGCCTGACC TGTTGAAGCA ATCACGTAGT 
ACCGTGCAGA GCTCAGAACC TGCTTGTGAC CCTGTGTCAT GGGAGGAATT AAAGTCGATG 
GACCTGCCAT CCTTTCTACC TGCTTTTTTA GTCCTCTGTC GGGTCCTTCT AAATGTTATT 
CATGAATGTC TCAAACTGAG ACTGGAGCAA AGGCCAGCTG GGGAACCATC ACTCCTGAGC 
ATTAAGCAGT TAGTGAGAGA GTGCAAAGAA GTTCTGAAGG GCGGACTTCT CATGAAACAA 
TATTATCAGT TCATGCTCCG AGAAGTGTTG GATGACCTGC AGAAATTGGA TTGTGACATT 
GATTCATTTG AAGATGATCT TCACAAAATG TTAATGGTGT ACTTTGACTA TATGCGGAGC 
TGGATTCAAA TGCTTCAACA GTTACCTCAA GCTTCACACA GTCTAAAAAA CCTGCTAGAG 
GAAGAGTGGA ATTTCACCAA AGAAATCACT CCACTTATAC GTGGGGGGGA AGCACAGGCA 
GGGAAGCTTT TTTGCGATAT TGCAGGAATG CTGCTGAAAT CCACTGGGAA ATTTCTTGAC 
TCTGGTCTTC TGGACAGCTG TGACGAATTC TGGGCCAGCA CTGATGATAG TAGCATCTCG 
GATGAAATAA GGAGATCTGT TATTGAAACC AGCCGAGCAC TGAAAGAGCT GTTTCATGAA 
GCCAGAGAGA GAGCATCCAA AGCACTTGGC TTTGCAAAGA TGCTGAGAAA GGATTTGGAA 
ATAGCTGCAG AATTTACTTT TGCCTCTACT ATTCTTGAAC TACTTGCATT TTTAAAAGCC 
AAGGATTATG CAAAGGTGCT CATCCCTTCT GTGGAAAACC TTCATGTGTT TATACCAGGT 
AATCTCTCTA GAGAAAAGAT CATTGTTCAG CAATTACTTG GTACTGCAGC TGGCATAGAC 
TGTTCCAAAG ACTGTGAGGA CATGGCATAT GACCAGTTCC TGCTCATGAC GAAGCATATC 
GATAAAGATA AACCTCAGGA GGATTGGAAC AGTTGGGATG GATGCTGTAT TAAAAGCATG 
CCACCAGTGG AAACAGCAGA TACTCTAAGA AGTATGCAGA CTGACAACCT TCTGCTGGTG 
GTAATGCAGT CAGCCCACCT CCAGAGTCAA AGAAAGGCTT TCCAGCACTC CACAGAAGGG 
CTTTTATCTT TGCGATTGGA GCAGACATCA AGCCATCCAA TGATTGCAGA GGCTTTGTTC 
CAACTCAAGA GTGAAGCTCT GCAACTCTGC AATGAAATTA GCAGTGCAAT TGACCGTGTT 
GATAAAATGT TCACCAATGA GTCTGAAGCA GAGATTGATG AATCAGAATC TTCTACTTTG 
CAGCAGTATT ACAGAGAAGC AATGATTCAA GGTTATAACT TTGGCTTTGA GTATCACAAA 
GAGGTGGTTC GTCTGATGTC TGGAGAGTTC AGACAGAAAA CTGGTGACAA ATATATCAAC 
TTTGCTAGAA AGTGGATGAA CTTTGTGCTG ACAAAATGTG AAAGTGGTCG CGGCACCAGA 
CCAAGGTGGG CAACCCAAGG TTTTGACTAT CTTCAAGCTA TTGAACCAGT ATTCATTTCA 
GCTCTCCCAG AGGATGACTT TTTGAGTCTA CAAGCTCTGA TGAATGAATG CATTGGCCAT 
GTTATTGGGA AACCCCACAG TCCTGTTACA GCTACTCATC GAAACAGCCC CCGTCCCGTG 
AAGGTGCCTC GATGCCACAG TGACCCACCA AATCCAAATC TGATCACTCC AACTCCTGAA 
GGCTTCAGGG GTTCCAGTGT TCCCGAAAAT GATCGTTTGG CCTCCGTAGC TTCAGAGTTA 
CAGTTCAGAT CCCTCAGTCG TCACTCTAGT CCAACAGAGG ACAGGGAAGA GCCGTCCTAT 
CCAAAAGGGG AGTTGTGGGG GTCAACTCGG CGAAGCTGGG AGCTTCAGAC ATTTATCAGT 
CAAACTCGAG ACATAGCTGC AAGACACCGG CCTATGAATG CTATACAGGA GTCGGTCAAA 
CAGTTTGAGG ATGAGCGCTA CCGGCAAATG AGAAATAAAA ATATTGTCGG CCAAGTCTGT 
GATACCCCAA AATCCTATGA CAATGTAATG CATGTTGGTC TTAGAAAAGT GACTTTCAAA 
TGGCAGAGAG GAAACAAAAT TGGGGAAGGG CAGTATGGCA AAGTGTACAC ATGTATTAGC 
ATTGACACAG GGGAACTGAT GGCAATGAAA GAAATACGTT TTCAGCCAAA TGATCACAAG 
ACTATAAAGG AAACTGCAGA TGAATTGAAA ATTTTTGAAG GAATCAAACA CCCAAATCTT 
GTTCGTTACT TTGGTGTCGA GCTCCACAGA GAAGAAATGT ATATTTTTAT GGAATACTGT 
GATGAAGGGA CTTTGGAGGA GGTATCTAGA CTGGGCCTGC AGGAGCATGT CATTAGACTT 
TATACAAAGC AGATAACCAT TGCTATAAAT GTCCTGCATG AGCATGGTAT TGTTCATCGC 
GATATTAAAG GAGCAAATAT ATTTCTTACA TCTTCTGGAC TCATAAAACT GGGCGATTTT 
GGATGTTCGG TCAAGCTGAA GAATAATGCC CAGACTATGC CTGGAGAAGT TAACAGCACA 
CTTGGAACTG CAGCATATAT GGCTCCTGAA GTGATAACCC AAGCTAAGGG AGAGGGGCAT 
GGGCGTGCTG CAGACATATG GAGCCTTGGC TGTGTTTTAA TTGAGATGGT TACTGGCAAG 
AGACCTTGGC ATGAATATGA ACATAACTTC CAGATCATGT ACCGAGTCGG TATGGGACAC 
AAGCCCCCCA TTCCTGATCG GCTGAGCCCA GAGGGTAAAG ACTTCCTTTC TCATTGCCAG 
GAAAGTGATC CAAAAATGCG ATGGACAGCT AGCCAACTCT TGGACCATGC ATTTGTCAAG 
GTTTGCACAG ATGAAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k4 ( XM_002936262.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936308.2 Xenopus tropicalis mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X1, mRNA.

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CloneID	OXa10659
Clone ID Related Accession (Same CDS sequence)	XM_002936262.4 , XM_002936262.5
Accession Version	XM_002936262.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002936262.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAAA CTGAAGATGC TACTGATGAC AATCTGGATT GTGAAGCTGA TGAGTTTTCA 
GATGAAGCAA GTGCAGAATC TCTCTATAAC ACCTTTCCGA CCAGCATTTC CCGACAGATG 
AAGCGAATGT CATCCAAGAA CCAGTGGAAT AATACAGGGA GGCCAGTTGG CCATTTCACG 
TTCAATGAAA AGATGAGTAG ACCAAATCCA TCACCTCACA AAGAGAGCAG CAAAACCACT 
GGAAATGTTG AGGATCATAA CTATAAGCAA GAGAAGAAGA ACAGAGCCAC TTTAAGAATG 
ACTGAGCGGG ATCAAAAAAA AAATGTGCAG TGCTCCTTTT TGCTTGACCC AGGAAGTGGG 
CCCCTGACAA AAAAATCTAT TCCAGATGTT GATCTTAATA AACCATATCT CAGTCTTGGC 
TGCAGTAGTT CCAAGCTACC TGTATCTGTA CCTGTTTCAT TAGCTAGGAC TACAAGGCAG 
AGCTCCAGGA CTGACTGCCC AGCTGACCGT CTCAAGTTCT TTGAGACACT GCGACTTTTG 
TTGAAACTAA CTTCCGTCTC TAAGAAAAAG GAGAGAGAAC AAAGGGGTCA GGAAAATACT 
TCTTCTATGG GGTATAACCA GTCTAATGAA CTTTTCTGGC TGGAACTGCA AGCTTGGCAT 
GCTGGTCGTA GTATTAATGA CCAAGACTTG TACCTATACA CAGCTCGACA GACAATTCCT 
GACATCATTA ATGAAATTTT AACTTTTAAG GTGAACTATG GGTCTTTATT TAGCGATGCT 
GATAACATCA ATGGGACATT TAGAGAAAGA CCATTTATTG TCTCACCAGG ATCTTTTGCT 
TTTGGCCACT CAAGTTATCA TGAACATATC CAGAAGCAAA AAATATCGTT TGAACAAGTA 
AAGCAGATTA TGGACCTGCT GGAATACATT GAGGCACTTT ACCCATCTTT GAATGCTCTT 
CAGAGGGATT ATGAAAAATA TGCTGCAAAA GATTTTCAGG CTAGAGTGCA GGCACTTTGC 
CTGTGGCTTA ATATAACCAA GGACTTGAAT CACAAATTGA GGATAATGGG ATCTGTTCTT 
GGTATCAAGA ACTTGTCAAA CATTGGCTGG CCAGTGTTTG AAATTTCTTC ACCATGTCCA 
TCAAATTGCC CAGAGGATGA ACATGACAAC ATAGAACATG AGGGACAAAT ACATGATGGA 
GCACCTAGAA TGTGTAGAGA GGAAAGAGAA GAAGAAGAGC TAACAATTAA CAATTTAGAT 
TTGAGACAAG ATTTTAACAG CAAGCTAACA GTTAATTTTT TTGAAGAAGA CTACTCCTCC 
TCAAACAAAC TAGATAAGAT TATGTCAGAG GATGATTTTA GCTGGGGAAA TCCAGAGTGC 
AATGATAGTG ATTGTAATAG GCATTGTTCA ACCTCCATTT ATAGACCTTT TGTTGACAAA 
GCTCTGAAAC AGATGGGACT GAGGAAACTT ATTCTGAGAC TGCATAAATT AATGCATGGT 
TCTCTTCAGA GAGCACGTAG AGCCTTAATG ATGGCTGATC GTACATTTGA TTTTTCAGAA 
TACCCTGACC TGATGTTATG TTCGGATTAC TTGCCTGACC TGTTGAAGCA ATCACGTAGT 
ACCGTGCAGA GCTCAGAACC TGCTTGTGAC CCTGTGTCAT GGGAGGAATT AAAGTCGATG 
GACCTGCCAT CCTTTCTACC TGCTTTTTTA GTCCTCTGTC GGGTCCTTCT AAATGTTATT 
CATGAATGTC TCAAACTGAG ACTGGAGCAA AGGCCAGCTG GGGAACCATC ACTCCTGAGC 
ATTAAGCAGT TAGTGAGAGA GTGCAAAGAA GTTCTGAAGG GCGGACTTCT CATGAAACAA 
TATTATCAGT TCATGCTCCG AGAAGTGTTG GATGACCTGC AGAAATTGGA TTGTGACATT 
GATTCATTTG AAGATGATCT TCACAAAATG TTAATGGTGT ACTTTGACTA TATGCGGAGC 
TGGATTCAAA TGCTTCAACA GTTACCTCAA GCTTCACACA GTCTAAAAAA CCTGCTAGAG 
GAAGAGTGGA ATTTCACCAA AGAAATCACT CCACTTATAC GTGGGGGGGA AGCACAGGCA 
GGGAAGCTTT TTTGCGATAT TGCAGGAATG CTGCTGAAAT CCACTGGGAA ATTTCTTGAC 
TCTGGTCTTC TGGACAGCTG TGACGAATTC TGGGCCAGCA CTGATGATAG TAGCATCTCG 
GATGAAATAA GGAGATCTGT TATTGAAACC AGCCGAGCAC TGAAAGAGCT GTTTCATGAA 
GCCAGAGAGA GAGCATCCAA AGCACTTGGC TTTGCAAAGA TGCTGAGAAA GGATTTGGAA 
ATAGCTGCAG AATTTACTTT TGCCTCTACT ATTCTTGAAC TACTTGCATT TTTAAAAGCC 
AAGGATTATG CAAAGGTGCT CATCCCTTCT GTGGAAAACC TTCATGTGTT TATACCAGGT 
AATCTCTCTA GAGAAAAGAT CATTGTTCAG CAATTACTTG GTACTGCAGC TGGCATAGAC 
TGTTCCAAAG ACTGTGAGGA CATGGCATAT GACCAGTTCC TGCTCATGAC GAAGCATATC 
GATAAAGATA AACCTCAGGA GGATTGGAAC AGTTGGGATG GATGCTGTAT TAAAAGCATG 
CCACCAGTGG AAACAGCAGA TACTCTAAGA AGTATGCAGA CTGACAACCT TCTGCTGGTG 
GTAATGCAGT CAGCCCACCT CCAGAGTCAA AGAAAGGCTT TCCAGCACTC CACAGAAGGG 
CTTTTATCTT TGCGATTGGA GCAGACATCA AGCCATCCAA TGATTGCAGA GGCTTTGTTC 
CAACTCAAGA GTGAAGCTCT GCAACTCTGC AATGAAATTA GCAGTGCAAT TGACCGTGTT 
GATAAAATGT TCACCAATGA GTCTGAAGCA GAGATTGATG AATCAGAATC TTCTACTTTG 
CAGCAGTATT ACAGAGAAGC AATGATTCAA GGTTATAACT TTGGCTTTGA GTATCACAAA 
GAGGTGGTTC GTCTGATGTC TGGAGAGTTC AGACAGAAAA CTGGTGACAA ATATATCAAC 
TTTGCTAGAA AGTGGATGAA CTTTGTGCTG ACAAAATGTG AAAGTGGTCG CGGCACCAGA 
CCAAGGTGGG CAACCCAAGG TTTTGACTAT CTTCAAGCTA TTGAACCAGT ATTCATTTCA 
GCTCTCCCAG AGGATGACTT TTTGAGTCTA CAAGCTCTGA TGAATGAATG CATTGGCCAT 
GTTATTGGGA AACCCCACAG TCCTGTTACA GGTTTGTACA TTGCTACTCA TCGAAACAGC 
CCCCGTCCCG TGAAGGTGCC TCGATGCCAC AGTGACCCAC CAAATCCAAA TCTGATCACT 
CCAACTCCTG AAGGCTTCAG GGGTTCCAGT GTTCCCGAAA ATGATCGTTT GGCCTCCGTA 
GCTTCAGAGT TACAGTTCAG ATCCCTCAGT CGTCACTCTA GTCCAACAGA GGACAGGGAA 
GAGCCGTCCT ATCCAAAAGG GGAGTTGTGG GGGTCAACTC GGCGAAGCTG GGAGCTTCAG 
ACATTTATCA GTCAAACTCG AGACATAGCT GCAAGACACC GGCCTATGAA TGCTATACAG 
GAGTCGGTCA AACAGTTTGA GGATGAGCGC TACCGGCAAA TGAGAAATAA AAATATTGTC 
GGCCAAGTCT GTGATACCCC AAAATCCTAT GACAATGTAA TGCATGTTGG TCTTAGAAAA 
GTGACTTTCA AATGGCAGAG AGGAAACAAA ATTGGGGAAG GGCAGTATGG CAAAGTGTAC 
ACATGTATTA GCATTGACAC AGGGGAACTG ATGGCAATGA AAGAAATACG TTTTCAGCCA 
AATGATCACA AGACTATAAA GGAAACTGCA GATGAATTGA AAATTTTTGA AGGAATCAAA 
CACCCAAATC TTGTTCGTTA CTTTGGTGTC GAGCTCCACA GAGAAGAAAT GTATATTTTT 
ATGGAATACT GTGATGAAGG GACTTTGGAG GAGGTATCTA GACTGGGCCT GCAGGAGCAT 
GTCATTAGAC TTTATACAAA GCAGATAACC ATTGCTATAA ATGTCCTGCA TGAGCATGGT 
ATTGTTCATC GCGATATTAA AGGAGCAAAT ATATTTCTTA CATCTTCTGG ACTCATAAAA 
CTGGGCGATT TTGGATGTTC GGTCAAGCTG AAGAATAATG CCCAGACTAT GCCTGGAGAA 
GTTAACAGCA CACTTGGAAC TGCAGCATAT ATGGCTCCTG AAGTGATAAC CCAAGCTAAG 
GGAGAGGGGC ATGGGCGTGC TGCAGACATA TGGAGCCTTG GCTGTGTTTT AATTGAGATG 
GTTACTGGCA AGAGACCTTG GCATGAATAT GAACATAACT TCCAGATCAT GTACCGAGTC 
GGTATGGGAC ACAAGCCCCC CATTCCTGAT CGGCTGAGCC CAGAGGGTAA AGACTTCCTT 
TCTCATTGCC AGGAAAGTGA TCCAAAAATG CGATGGACAG CTAGCCAACT CTTGGACCAT 
GCATTTGTCA AGGTTTGCAC AGATGAAGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936308.2
CDS	94..4626
Translation	MKKTEDATDDNLDCEADEFSDEASAESLYNTFPTSISRQMKRMSSKNQWNNTGRPVGHFTFNEKMSRPNPSPHKESSKTTGNVEDHNYKQEKKNRATLRMTERDQKKNVQCSFLLDPGSGPLTKKSIPDVDLNKPYLSLGCSSSKLPVSVPVSLARTTRQSSRTDCPADRLKFFETLRLLLKLTSVSKKKEREQRGQENTSSMGYNQSNELFWLELQAWHAGRSINDQDLYLYTARQTIPDIINEILTFKVNYGSLFSDADNINGTFRERPFIVSPGSFAFGHSSYHEHIQKQKISFEQVKQIMDLLEYIEALYPSLNALQRDYEKYAAKDFQARVQALCLWLNITKDLNHKLRIMGSVLGIKNLSNIGWPVFEISSPCPSNCPEDEHDNIEHEGQIHDGAPRMCREEREEEELTINNLDLRQDFNSKLTVNFFEEDYSSSNKLDKIMSEDDFSWGNPECNDSDCNRHCSTSIYRPFVDKALKQMGLRKLILRLHKLMHGSLQRARRALMMADRTFDFSEYPDLMLCSDYLPDLLKQSRSTVQSSEPACDPVSWEELKSMDLPSFLPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLREVLDDLQKLDCDIDSFEDDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITPLIRGGEAQAGKLFCDIAGMLLKSTGKFLDSGLLDSCDEFWASTDDSSISDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFTFASTILELLAFLKAKDYAKVLIPSVENLHVFIPGNLSREKIIVQQLLGTAAGIDCSKDCEDMAYDQFLLMTKHIDKDKPQEDWNSWDGCCIKSMPPVETADTLRSMQTDNLLLVVMQSAHLQSQRKAFQHSTEGLLSLRLEQTSSHPMIAEALFQLKSEALQLCNEISSAIDRVDKMFTNESEAEIDESESSTLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKTGDKYINFARKWMNFVLTKCESGRGTRPRWATQGFDYLQAIEPVFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYIATHRNSPRPVKVPRCHSDPPNPNLITPTPEGFRGSSVPENDRLASVASELQFRSLSRHSSPTEDREEPSYPKGELWGSTRRSWELQTFISQTRDIAARHRPMNAIQESVKQFEDERYRQMRNKNIVGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISIDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYTKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITQAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYRVGMGHKPPIPDRLSPEGKDFLSHCQESDPKMRWTASQLLDHAFVKVCTDEE

Target ORF information:

RefSeq Version	XM_002936262.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936262.4

ORF Insert Sequence:

ATGAAAAAAA CTGAAGATGC TACTGATGAC AATCTGGATT GTGAAGCTGA TGAGTTTTCA 
GATGAAGCAA GTGCAGAATC TCTCTATAAC ACCTTTCCGA CCAGCATTTC CCGACAGATG 
AAGCGAATGT CATCCAAGAA CCAGTGGAAT AATACAGGGA GGCCAGTTGG CCATTTCACG 
TTCAATGAAA AGATGAGTAG ACCAAATCCA TCACCTCACA AAGAGAGCAG CAAAACCACT 
GGAAATGTTG AGGATCATAA CTATAAGCAA GAGAAGAAGA ACAGAGCCAC TTTAAGAATG 
ACTGAGCGGG ATCAAAAAAA AAATGTGCAG TGCTCCTTTT TGCTTGACCC AGGAAGTGGG 
CCCCTGACAA AAAAATCTAT TCCAGATGTT GATCTTAATA AACCATATCT CAGTCTTGGC 
TGCAGTAGTT CCAAGCTACC TGTATCTGTA CCTGTTTCAT TAGCTAGGAC TACAAGGCAG 
AGCTCCAGGA CTGACTGCCC AGCTGACCGT CTCAAGTTCT TTGAGACACT GCGACTTTTG 
TTGAAACTAA CTTCCGTCTC TAAGAAAAAG GAGAGAGAAC AAAGGGGTCA GGAAAATACT 
TCTTCTATGG GGTATAACCA GTCTAATGAA CTTTTCTGGC TGGAACTGCA AGCTTGGCAT 
GCTGGTCGTA GTATTAATGA CCAAGACTTG TACCTATACA CAGCTCGACA GACAATTCCT 
GACATCATTA ATGAAATTTT AACTTTTAAG GTGAACTATG GGTCTTTATT TAGCGATGCT 
GATAACATCA ATGGGACATT TAGAGAAAGA CCATTTATTG TCTCACCAGG ATCTTTTGCT 
TTTGGCCACT CAAGTTATCA TGAACATATC CAGAAGCAAA AAATATCGTT TGAACAAGTA 
AAGCAGATTA TGGACCTGCT GGAATACATT GAGGCACTTT ACCCATCTTT GAATGCTCTT 
CAGAGGGATT ATGAAAAATA TGCTGCAAAA GATTTTCAGG CTAGAGTGCA GGCACTTTGC 
CTGTGGCTTA ATATAACCAA GGACTTGAAT CACAAATTGA GGATAATGGG ATCTGTTCTT 
GGTATCAAGA ACTTGTCAAA CATTGGCTGG CCAGTGTTTG AAATTTCTTC ACCATGTCCA 
TCAAATTGCC CAGAGGATGA ACATGACAAC ATAGAACATG AGGGACAAAT ACATGATGGA 
GCACCTAGAA TGTGTAGAGA GGAAAGAGAA GAAGAAGAGC TAACAATTAA CAATTTAGAT 
TTGAGACAAG ATTTTAACAG CAAGCTAACA GTTAATTTTT TTGAAGAAGA CTACTCCTCC 
TCAAACAAAC TAGATAAGAT TATGTCAGAG GATGATTTTA GCTGGGGAAA TCCAGAGTGC 
AATGATAGTG ATTGTAATAG GCATTGTTCA ACCTCCATTT ATAGACCTTT TGTTGACAAA 
GCTCTGAAAC AGATGGGACT GAGGAAACTT ATTCTGAGAC TGCATAAATT AATGCATGGT 
TCTCTTCAGA GAGCACGTAG AGCCTTAATG ATGGCTGATC GTACATTTGA TTTTTCAGAA 
TACCCTGACC TGATGTTATG TTCGGATTAC TTGCCTGACC TGTTGAAGCA ATCACGTAGT 
ACCGTGCAGA GCTCAGAACC TGCTTGTGAC CCTGTGTCAT GGGAGGAATT AAAGTCGATG 
GACCTGCCAT CCTTTCTACC TGCTTTTTTA GTCCTCTGTC GGGTCCTTCT AAATGTTATT 
CATGAATGTC TCAAACTGAG ACTGGAGCAA AGGCCAGCTG GGGAACCATC ACTCCTGAGC 
ATTAAGCAGT TAGTGAGAGA GTGCAAAGAA GTTCTGAAGG GCGGACTTCT CATGAAACAA 
TATTATCAGT TCATGCTCCG AGAAGTGTTG GATGACCTGC AGAAATTGGA TTGTGACATT 
GATTCATTTG AAGATGATCT TCACAAAATG TTAATGGTGT ACTTTGACTA TATGCGGAGC 
TGGATTCAAA TGCTTCAACA GTTACCTCAA GCTTCACACA GTCTAAAAAA CCTGCTAGAG 
GAAGAGTGGA ATTTCACCAA AGAAATCACT CCACTTATAC GTGGGGGGGA AGCACAGGCA 
GGGAAGCTTT TTTGCGATAT TGCAGGAATG CTGCTGAAAT CCACTGGGAA ATTTCTTGAC 
TCTGGTCTTC TGGACAGCTG TGACGAATTC TGGGCCAGCA CTGATGATAG TAGCATCTCG 
GATGAAATAA GGAGATCTGT TATTGAAACC AGCCGAGCAC TGAAAGAGCT GTTTCATGAA 
GCCAGAGAGA GAGCATCCAA AGCACTTGGC TTTGCAAAGA TGCTGAGAAA GGATTTGGAA 
ATAGCTGCAG AATTTACTTT TGCCTCTACT ATTCTTGAAC TACTTGCATT TTTAAAAGCC 
AAGGATTATG CAAAGGTGCT CATCCCTTCT GTGGAAAACC TTCATGTGTT TATACCAGGT 
AATCTCTCTA GAGAAAAGAT CATTGTTCAG CAATTACTTG GTACTGCAGC TGGCATAGAC 
TGTTCCAAAG ACTGTGAGGA CATGGCATAT GACCAGTTCC TGCTCATGAC GAAGCATATC 
GATAAAGATA AACCTCAGGA GGATTGGAAC AGTTGGGATG GATGCTGTAT TAAAAGCATG 
CCACCAGTGG AAACAGCAGA TACTCTAAGA AGTATGCAGA CTGACAACCT TCTGCTGGTG 
GTAATGCAGT CAGCCCACCT CCAGAGTCAA AGAAAGGCTT TCCAGCACTC CACAGAAGGG 
CTTTTATCTT TGCGATTGGA GCAGACATCA AGCCATCCAA TGATTGCAGA GGCTTTGTTC 
CAACTCAAGA GTGAAGCTCT GCAACTCTGC AATGAAATTA GCAGTGCAAT TGACCGTGTT 
GATAAAATGT TCACCAATGA GTCTGAAGCA GAGATTGATG AATCAGAATC TTCTACTTTG 
CAGCAGTATT ACAGAGAAGC AATGATTCAA GGTTATAACT TTGGCTTTGA GTATCACAAA 
GAGGTGGTTC GTCTGATGTC TGGAGAGTTC AGACAGAAAA CTGGTGACAA ATATATCAAC 
TTTGCTAGAA AGTGGATGAA CTTTGTGCTG ACAAAATGTG AAAGTGGTCG CGGCACCAGA 
CCAAGGTGGG CAACCCAAGG TTTTGACTAT CTTCAAGCTA TTGAACCAGT ATTCATTTCA 
GCTCTCCCAG AGGATGACTT TTTGAGTCTA CAAGCTCTGA TGAATGAATG CATTGGCCAT 
GTTATTGGGA AACCCCACAG TCCTGTTACA GGTTTGTACA TTGCTACTCA TCGAAACAGC 
CCCCGTCCCG TGAAGGTGCC TCGATGCCAC AGTGACCCAC CAAATCCAAA TCTGATCACT 
CCAACTCCTG AAGGCTTCAG GGGTTCCAGT GTTCCCGAAA ATGATCGTTT GGCCTCCGTA 
GCTTCAGAGT TACAGTTCAG ATCCCTCAGT CGTCACTCTA GTCCAACAGA GGACAGGGAA 
GAGCCGTCCT ATCCAAAAGG GGAGTTGTGG GGGTCAACTC GGCGAAGCTG GGAGCTTCAG 
ACATTTATCA GTCAAACTCG AGACATAGCT GCAAGACACC GGCCTATGAA TGCTATACAG 
GAGTCGGTCA AACAGTTTGA GGATGAGCGC TACCGGCAAA TGAGAAATAA AAATATTGTC 
GGCCAAGTCT GTGATACCCC AAAATCCTAT GACAATGTAA TGCATGTTGG TCTTAGAAAA 
GTGACTTTCA AATGGCAGAG AGGAAACAAA ATTGGGGAAG GGCAGTATGG CAAAGTGTAC 
ACATGTATTA GCATTGACAC AGGGGAACTG ATGGCAATGA AAGAAATACG TTTTCAGCCA 
AATGATCACA AGACTATAAA GGAAACTGCA GATGAATTGA AAATTTTTGA AGGAATCAAA 
CACCCAAATC TTGTTCGTTA CTTTGGTGTC GAGCTCCACA GAGAAGAAAT GTATATTTTT 
ATGGAATACT GTGATGAAGG GACTTTGGAG GAGGTATCTA GACTGGGCCT GCAGGAGCAT 
GTCATTAGAC TTTATACAAA GCAGATAACC ATTGCTATAA ATGTCCTGCA TGAGCATGGT 
ATTGTTCATC GCGATATTAA AGGAGCAAAT ATATTTCTTA CATCTTCTGG ACTCATAAAA 
CTGGGCGATT TTGGATGTTC GGTCAAGCTG AAGAATAATG CCCAGACTAT GCCTGGAGAA 
GTTAACAGCA CACTTGGAAC TGCAGCATAT ATGGCTCCTG AAGTGATAAC CCAAGCTAAG 
GGAGAGGGGC ATGGGCGTGC TGCAGACATA TGGAGCCTTG GCTGTGTTTT AATTGAGATG 
GTTACTGGCA AGAGACCTTG GCATGAATAT GAACATAACT TCCAGATCAT GTACCGAGTC 
GGTATGGGAC ACAAGCCCCC CATTCCTGAT CGGCTGAGCC CAGAGGGTAA AGACTTCCTT 
TCTCATTGCC AGGAAAGTGA TCCAAAAATG CGATGGACAG CTAGCCAACT CTTGGACCAT 
GCATTTGTCA AGGTTTGCAC AGATGAAGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k4 ( XM_004914648.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914705.1 Xenopus tropicalis mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X2, mRNA.

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CloneID	OXa10660
Clone ID Related Accession (Same CDS sequence)	XM_004914648.4 , XM_004914648.3
Accession Version	XM_004914648.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914648.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAAA CTGAAGATGC TACTGATGAC AATCTGGATT GTGAAGCTGA TGAGTTTTCA 
GATGAAGCAA GTGCAGAATC TCTCTATAAC ACCTTTCCGA CCAGCATTTC CCGACAGATG 
AAGCGAATGT CATCCAAGAA CCAGTGGAAT AATACAGGGA GGCCAGTTGG CCATTTCACG 
TTCAATGAAA AGATGAGTAG ACCAAATCCA TCACCTCACA AAGAGAGCAG CAAAACCACT 
GGAAATGTTG AGGATCATAA CTATAAGCAA GAGAAGAAGA ACAGAGCCAC TTTAAGAATG 
ACTGAGCGGG ATCAAAAAAA AAATGTGCAG TGCTCCTTTT TGCTTGACCC AGGAAGTGGG 
CCCCTGACAA AAAAATCTAT TCCAGATGTT GATCTTAATA AACCATATCT CAGTCTTGGC 
TGCAGTAGTT CCAAGCTACC TGTATCTGTA CCTGTTTCAT TAGCTAGGAC TACAAGGCAG 
AGCTCCAGGA CTGACTGCCC AGCTGACCGT CTCAAGTTCT TTGAGACACT GCGACTTTTG 
TTGAAACTAA CTTCCGTCTC TAAGAAAAAG GAGAGAGAAC AAAGGGGTCA GGAAAATACT 
TCTTCTATGG GGTATAACCA GTCTAATGAA CTTTTCTGGC TGGAACTGCA AGCTTGGCAT 
GCTGGTCGTA GTATTAATGA CCAAGACTTG TACCTATACA CAGCTCGACA GACAATTCCT 
GACATCATTA ATGAAATTTT AACTTTTAAG GTGAACTATG GGTCTTTATT TAGCGATGCT 
GATAACATCA ATGGGACATT TAGAGAAAGA CCATTTATTG TCTCACCAGG ATCTTTTGCT 
TTTGGCCACT CAAGTTATCA TGAACATATC CAGAAGCAAA AAATATCGTT TGAACAAGTA 
AAGCAGATTA TGGACCTGCT GGAATACATT GAGGCACTTT ACCCATCTTT GAATGCTCTT 
CAGAGGGATT ATGAAAAATA TGCTGCAAAA GATTTTCAGG CTAGAGTGCA GGCACTTTGC 
CTGTGGCTTA ATATAACCAA GGACTTGAAT CACAAATTGA GGATAATGGG ATCTGTTCTT 
GGTATCAAGA ACTTGTCAAA CATTGGCTGG CCAGTGTTTG AAATTTCTTC ACCATGTCCA 
TCAAATTGCC CAGAGGATGA ACATGACAAC ATAGAACATG AGGGACAAAT ACATGATGGA 
GCACCTAGAA TGTGTAGAGA GGAAAGAGAA GAAGAAGAGC TAACAATTAA CAATTTAGAT 
TTGAGACAAG ATTTTAACAG CAAGCTAACA GTTAATTTTT TTGAAGAAGA CTACTCCTCC 
TCAAACAAAC TAGATAAGAT TATGTCAGAG GATGATTTTA GCTGGGGAAA TCCAGAGTGC 
AATGATAGTG ATTGTAATAG GCATTGTTCA ACCTCCATTT ATAGACCTTT TGTTGACAAA 
GCTCTGAAAC AGATGGGACT GAGGAAACTT ATTCTGAGAC TGCATAAATT AATGCATGGT 
TCTCTTCAGA GAGCACGTAG AGCCTTAATG ATGGCTGATC GTACATTTGA TTTTTCAGAA 
TACCCTGACC TGATGTTATG TTCGGATTAC TTGCCTGACC TGTTGAAGCA ATCACGTAGT 
ACCGTGCAGA GCTCAGAACC TGCTTGTGAC CCTGTGTCAT GGGAGGAATT AAAGTCGATG 
GACCTGCCAT CCTTTCTACC TGCTTTTTTA GTCCTCTGTC GGGTCCTTCT AAATGTTATT 
CATGAATGTC TCAAACTGAG ACTGGAGCAA AGGCCAGCTG GGGAACCATC ACTCCTGAGC 
ATTAAGCAGT TAGTGAGAGA GTGCAAAGAA GTTCTGAAGG GCGGACTTCT CATGAAACAA 
TATTATCAGT TCATGCTCCG AGAAGTGTTG GATGACCTGC AGAAATTGGA TTGTGACATT 
GATTCATTTG AAGATGATCT TCACAAAATG TTAATGGTGT ACTTTGACTA TATGCGGAGC 
TGGATTCAAA TGCTTCAACA GTTACCTCAA GCTTCACACA GTCTAAAAAA CCTGCTAGAG 
GAAGAGTGGA ATTTCACCAA AGAAATCACT CCACTTATAC GTGGGGGGGA AGCACAGGCA 
GGGAAGCTTT TTTGCGATAT TGCAGGAATG CTGCTGAAAT CCACTGGGAA ATTTCTTGAC 
TCTGGTCTTC TGGACAGCTG TGACGAATTC TGGGCCAGCA CTGATGATAG TAGCATCTCG 
GATGAAATAA GGAGATCTGT TATTGAAACC AGCCGAGCAC TGAAAGAGCT GTTTCATGAA 
GCCAGAGAGA GAGCATCCAA AGCACTTGGC TTTGCAAAGA TGCTGAGAAA GGATTTGGAA 
ATAGCTGCAG AATTTACTTT TGCCTCTACT ATTCTTGAAC TACTTGCATT TTTAAAAGCC 
AAGGATTATG CAAAGGTGCT CATCCCTTCT GTGGAAAACC TTCATGTGTT TATACCAGGT 
AATCTCTCTA GAGAAAAGAT CATTGTTCAG CAATTACTTG GTACTGCAGC TGGCATAGAC 
TGTTCCAAAG ACTGTGAGGA CATGGCATAT GACCAGTTCC TGCTCATGAC GAAGCATATC 
GATAAAGATA AACCTCAGGA GGATTGGAAC AGTTGGGATG GATGCTGTAT TAAAAGCATG 
CCACCAGTGG AAACAGCAGA TACTCTAAGA AGTATGCAGA CTGACAACCT TCTGCTGGTG 
GTAATGCAGT CAGCCCACCT CCAGAGTCAA AGAAAGGCTT TCCAGCACTC CACAGAAGGG 
CTTTTATCTT TGCGATTGGA GCAGACATCA AGCCATCCAA TGATTGCAGA GGCTTTGTTC 
CAACTCAAGA GTGAAGCTCT GCAACTCTGC AATGAAATTA GCAGTGCAAT TGACCGTGTT 
GATAAAATGT TCACCAATGA GTCTGAAGCA GAGATTGATG AATCAGAATC TTCTACTTTG 
CAGCAGTATT ACAGAGAAGC AATGATTCAA GGTTATAACT TTGGCTTTGA GTATCACAAA 
GAGGTGGTTC GTCTGATGTC TGGAGAGTTC AGACAGAAAA CTGGTGACAA ATATATCAAC 
TTTGCTAGAA AGTGGATGAA CTTTGTGCTG ACAAAATGTG AAAGTGGTCG CGGCACCAGA 
CCAAGGTGGG CAACCCAAGG TTTTGACTAT CTTCAAGCTA TTGAACCAGT ATTCATTTCA 
GCTCTCCCAG AGGATGACTT TTTGAGTCTA CAAGCTCTGA TGAATGAATG CATTGGCCAT 
GTTATTGGGA AACCCCACAG TCCTGTTACA GCTACTCATC GAAACAGCCC CCGTCCCGTG 
AAGGTGCCTC GATGCCACAG TGACCCACCA AATCCAAATC TGATCACTCC AACTCCTGAA 
GGCTTCAGGG GTTCCAGTGT TCCCGAAAAT GATCGTTTGG CCTCCGTAGC TTCAGAGTTA 
CAGTTCAGAT CCCTCAGTCG TCACTCTAGT CCAACAGAGG ACAGGGAAGA GCCGTCCTAT 
CCAAAAGGGG AGTTGTGGGG GTCAACTCGG CGAAGCTGGG AGCTTCAGAC ATTTATCAGT 
CAAACTCGAG ACATAGCTGC AAGACACCGG CCTATGAATG CTATACAGGA GTCGGTCAAA 
CAGTTTGAGG ATGAGCGCTA CCGGCAAATG AGAAATAAAA ATATTGTCGG CCAAGTCTGT 
GATACCCCAA AATCCTATGA CAATGTAATG CATGTTGGTC TTAGAAAAGT GACTTTCAAA 
TGGCAGAGAG GAAACAAAAT TGGGGAAGGG CAGTATGGCA AAGTGTACAC ATGTATTAGC 
ATTGACACAG GGGAACTGAT GGCAATGAAA GAAATACGTT TTCAGCCAAA TGATCACAAG 
ACTATAAAGG AAACTGCAGA TGAATTGAAA ATTTTTGAAG GAATCAAACA CCCAAATCTT 
GTTCGTTACT TTGGTGTCGA GCTCCACAGA GAAGAAATGT ATATTTTTAT GGAATACTGT 
GATGAAGGGA CTTTGGAGGA GGTATCTAGA CTGGGCCTGC AGGAGCATGT CATTAGACTT 
TATACAAAGC AGATAACCAT TGCTATAAAT GTCCTGCATG AGCATGGTAT TGTTCATCGC 
GATATTAAAG GAGCAAATAT ATTTCTTACA TCTTCTGGAC TCATAAAACT GGGCGATTTT 
GGATGTTCGG TCAAGCTGAA GAATAATGCC CAGACTATGC CTGGAGAAGT TAACAGCACA 
CTTGGAACTG CAGCATATAT GGCTCCTGAA GTGATAACCC AAGCTAAGGG AGAGGGGCAT 
GGGCGTGCTG CAGACATATG GAGCCTTGGC TGTGTTTTAA TTGAGATGGT TACTGGCAAG 
AGACCTTGGC ATGAATATGA ACATAACTTC CAGATCATGT ACCGAGTCGG TATGGGACAC 
AAGCCCCCCA TTCCTGATCG GCTGAGCCCA GAGGGTAAAG ACTTCCTTTC TCATTGCCAG 
GAAAGTGATC CAAAAATGCG ATGGACAGCT AGCCAACTCT TGGACCATGC ATTTGTCAAG 
GTTTGCACAG ATGAAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914705.1
CDS	94..4614
Translation	MKKTEDATDDNLDCEADEFSDEASAESLYNTFPTSISRQMKRMSSKNQWNNTGRPVGHFTFNEKMSRPNPSPHKESSKTTGNVEDHNYKQEKKNRATLRMTERDQKKNVQCSFLLDPGSGPLTKKSIPDVDLNKPYLSLGCSSSKLPVSVPVSLARTTRQSSRTDCPADRLKFFETLRLLLKLTSVSKKKEREQRGQENTSSMGYNQSNELFWLELQAWHAGRSINDQDLYLYTARQTIPDIINEILTFKVNYGSLFSDADNINGTFRERPFIVSPGSFAFGHSSYHEHIQKQKISFEQVKQIMDLLEYIEALYPSLNALQRDYEKYAAKDFQARVQALCLWLNITKDLNHKLRIMGSVLGIKNLSNIGWPVFEISSPCPSNCPEDEHDNIEHEGQIHDGAPRMCREEREEEELTINNLDLRQDFNSKLTVNFFEEDYSSSNKLDKIMSEDDFSWGNPECNDSDCNRHCSTSIYRPFVDKALKQMGLRKLILRLHKLMHGSLQRARRALMMADRTFDFSEYPDLMLCSDYLPDLLKQSRSTVQSSEPACDPVSWEELKSMDLPSFLPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLREVLDDLQKLDCDIDSFEDDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITPLIRGGEAQAGKLFCDIAGMLLKSTGKFLDSGLLDSCDEFWASTDDSSISDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFTFASTILELLAFLKAKDYAKVLIPSVENLHVFIPGNLSREKIIVQQLLGTAAGIDCSKDCEDMAYDQFLLMTKHIDKDKPQEDWNSWDGCCIKSMPPVETADTLRSMQTDNLLLVVMQSAHLQSQRKAFQHSTEGLLSLRLEQTSSHPMIAEALFQLKSEALQLCNEISSAIDRVDKMFTNESEAEIDESESSTLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKTGDKYINFARKWMNFVLTKCESGRGTRPRWATQGFDYLQAIEPVFISALPEDDFLSLQALMNECIGHVIGKPHSPVTATHRNSPRPVKVPRCHSDPPNPNLITPTPEGFRGSSVPENDRLASVASELQFRSLSRHSSPTEDREEPSYPKGELWGSTRRSWELQTFISQTRDIAARHRPMNAIQESVKQFEDERYRQMRNKNIVGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISIDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYTKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITQAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYRVGMGHKPPIPDRLSPEGKDFLSHCQESDPKMRWTASQLLDHAFVKVCTDEE

Target ORF information:

RefSeq Version	XM_004914648.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914648.3

ORF Insert Sequence:

ATGAAAAAAA CTGAAGATGC TACTGATGAC AATCTGGATT GTGAAGCTGA TGAGTTTTCA 
GATGAAGCAA GTGCAGAATC TCTCTATAAC ACCTTTCCGA CCAGCATTTC CCGACAGATG 
AAGCGAATGT CATCCAAGAA CCAGTGGAAT AATACAGGGA GGCCAGTTGG CCATTTCACG 
TTCAATGAAA AGATGAGTAG ACCAAATCCA TCACCTCACA AAGAGAGCAG CAAAACCACT 
GGAAATGTTG AGGATCATAA CTATAAGCAA GAGAAGAAGA ACAGAGCCAC TTTAAGAATG 
ACTGAGCGGG ATCAAAAAAA AAATGTGCAG TGCTCCTTTT TGCTTGACCC AGGAAGTGGG 
CCCCTGACAA AAAAATCTAT TCCAGATGTT GATCTTAATA AACCATATCT CAGTCTTGGC 
TGCAGTAGTT CCAAGCTACC TGTATCTGTA CCTGTTTCAT TAGCTAGGAC TACAAGGCAG 
AGCTCCAGGA CTGACTGCCC AGCTGACCGT CTCAAGTTCT TTGAGACACT GCGACTTTTG 
TTGAAACTAA CTTCCGTCTC TAAGAAAAAG GAGAGAGAAC AAAGGGGTCA GGAAAATACT 
TCTTCTATGG GGTATAACCA GTCTAATGAA CTTTTCTGGC TGGAACTGCA AGCTTGGCAT 
GCTGGTCGTA GTATTAATGA CCAAGACTTG TACCTATACA CAGCTCGACA GACAATTCCT 
GACATCATTA ATGAAATTTT AACTTTTAAG GTGAACTATG GGTCTTTATT TAGCGATGCT 
GATAACATCA ATGGGACATT TAGAGAAAGA CCATTTATTG TCTCACCAGG ATCTTTTGCT 
TTTGGCCACT CAAGTTATCA TGAACATATC CAGAAGCAAA AAATATCGTT TGAACAAGTA 
AAGCAGATTA TGGACCTGCT GGAATACATT GAGGCACTTT ACCCATCTTT GAATGCTCTT 
CAGAGGGATT ATGAAAAATA TGCTGCAAAA GATTTTCAGG CTAGAGTGCA GGCACTTTGC 
CTGTGGCTTA ATATAACCAA GGACTTGAAT CACAAATTGA GGATAATGGG ATCTGTTCTT 
GGTATCAAGA ACTTGTCAAA CATTGGCTGG CCAGTGTTTG AAATTTCTTC ACCATGTCCA 
TCAAATTGCC CAGAGGATGA ACATGACAAC ATAGAACATG AGGGACAAAT ACATGATGGA 
GCACCTAGAA TGTGTAGAGA GGAAAGAGAA GAAGAAGAGC TAACAATTAA CAATTTAGAT 
TTGAGACAAG ATTTTAACAG CAAGCTAACA GTTAATTTTT TTGAAGAAGA CTACTCCTCC 
TCAAACAAAC TAGATAAGAT TATGTCAGAG GATGATTTTA GCTGGGGAAA TCCAGAGTGC 
AATGATAGTG ATTGTAATAG GCATTGTTCA ACCTCCATTT ATAGACCTTT TGTTGACAAA 
GCTCTGAAAC AGATGGGACT GAGGAAACTT ATTCTGAGAC TGCATAAATT AATGCATGGT 
TCTCTTCAGA GAGCACGTAG AGCCTTAATG ATGGCTGATC GTACATTTGA TTTTTCAGAA 
TACCCTGACC TGATGTTATG TTCGGATTAC TTGCCTGACC TGTTGAAGCA ATCACGTAGT 
ACCGTGCAGA GCTCAGAACC TGCTTGTGAC CCTGTGTCAT GGGAGGAATT AAAGTCGATG 
GACCTGCCAT CCTTTCTACC TGCTTTTTTA GTCCTCTGTC GGGTCCTTCT AAATGTTATT 
CATGAATGTC TCAAACTGAG ACTGGAGCAA AGGCCAGCTG GGGAACCATC ACTCCTGAGC 
ATTAAGCAGT TAGTGAGAGA GTGCAAAGAA GTTCTGAAGG GCGGACTTCT CATGAAACAA 
TATTATCAGT TCATGCTCCG AGAAGTGTTG GATGACCTGC AGAAATTGGA TTGTGACATT 
GATTCATTTG AAGATGATCT TCACAAAATG TTAATGGTGT ACTTTGACTA TATGCGGAGC 
TGGATTCAAA TGCTTCAACA GTTACCTCAA GCTTCACACA GTCTAAAAAA CCTGCTAGAG 
GAAGAGTGGA ATTTCACCAA AGAAATCACT CCACTTATAC GTGGGGGGGA AGCACAGGCA 
GGGAAGCTTT TTTGCGATAT TGCAGGAATG CTGCTGAAAT CCACTGGGAA ATTTCTTGAC 
TCTGGTCTTC TGGACAGCTG TGACGAATTC TGGGCCAGCA CTGATGATAG TAGCATCTCG 
GATGAAATAA GGAGATCTGT TATTGAAACC AGCCGAGCAC TGAAAGAGCT GTTTCATGAA 
GCCAGAGAGA GAGCATCCAA AGCACTTGGC TTTGCAAAGA TGCTGAGAAA GGATTTGGAA 
ATAGCTGCAG AATTTACTTT TGCCTCTACT ATTCTTGAAC TACTTGCATT TTTAAAAGCC 
AAGGATTATG CAAAGGTGCT CATCCCTTCT GTGGAAAACC TTCATGTGTT TATACCAGGT 
AATCTCTCTA GAGAAAAGAT CATTGTTCAG CAATTACTTG GTACTGCAGC TGGCATAGAC 
TGTTCCAAAG ACTGTGAGGA CATGGCATAT GACCAGTTCC TGCTCATGAC GAAGCATATC 
GATAAAGATA AACCTCAGGA GGATTGGAAC AGTTGGGATG GATGCTGTAT TAAAAGCATG 
CCACCAGTGG AAACAGCAGA TACTCTAAGA AGTATGCAGA CTGACAACCT TCTGCTGGTG 
GTAATGCAGT CAGCCCACCT CCAGAGTCAA AGAAAGGCTT TCCAGCACTC CACAGAAGGG 
CTTTTATCTT TGCGATTGGA GCAGACATCA AGCCATCCAA TGATTGCAGA GGCTTTGTTC 
CAACTCAAGA GTGAAGCTCT GCAACTCTGC AATGAAATTA GCAGTGCAAT TGACCGTGTT 
GATAAAATGT TCACCAATGA GTCTGAAGCA GAGATTGATG AATCAGAATC TTCTACTTTG 
CAGCAGTATT ACAGAGAAGC AATGATTCAA GGTTATAACT TTGGCTTTGA GTATCACAAA 
GAGGTGGTTC GTCTGATGTC TGGAGAGTTC AGACAGAAAA CTGGTGACAA ATATATCAAC 
TTTGCTAGAA AGTGGATGAA CTTTGTGCTG ACAAAATGTG AAAGTGGTCG CGGCACCAGA 
CCAAGGTGGG CAACCCAAGG TTTTGACTAT CTTCAAGCTA TTGAACCAGT ATTCATTTCA 
GCTCTCCCAG AGGATGACTT TTTGAGTCTA CAAGCTCTGA TGAATGAATG CATTGGCCAT 
GTTATTGGGA AACCCCACAG TCCTGTTACA GCTACTCATC GAAACAGCCC CCGTCCCGTG 
AAGGTGCCTC GATGCCACAG TGACCCACCA AATCCAAATC TGATCACTCC AACTCCTGAA 
GGCTTCAGGG GTTCCAGTGT TCCCGAAAAT GATCGTTTGG CCTCCGTAGC TTCAGAGTTA 
CAGTTCAGAT CCCTCAGTCG TCACTCTAGT CCAACAGAGG ACAGGGAAGA GCCGTCCTAT 
CCAAAAGGGG AGTTGTGGGG GTCAACTCGG CGAAGCTGGG AGCTTCAGAC ATTTATCAGT 
CAAACTCGAG ACATAGCTGC AAGACACCGG CCTATGAATG CTATACAGGA GTCGGTCAAA 
CAGTTTGAGG ATGAGCGCTA CCGGCAAATG AGAAATAAAA ATATTGTCGG CCAAGTCTGT 
GATACCCCAA AATCCTATGA CAATGTAATG CATGTTGGTC TTAGAAAAGT GACTTTCAAA 
TGGCAGAGAG GAAACAAAAT TGGGGAAGGG CAGTATGGCA AAGTGTACAC ATGTATTAGC 
ATTGACACAG GGGAACTGAT GGCAATGAAA GAAATACGTT TTCAGCCAAA TGATCACAAG 
ACTATAAAGG AAACTGCAGA TGAATTGAAA ATTTTTGAAG GAATCAAACA CCCAAATCTT 
GTTCGTTACT TTGGTGTCGA GCTCCACAGA GAAGAAATGT ATATTTTTAT GGAATACTGT 
GATGAAGGGA CTTTGGAGGA GGTATCTAGA CTGGGCCTGC AGGAGCATGT CATTAGACTT 
TATACAAAGC AGATAACCAT TGCTATAAAT GTCCTGCATG AGCATGGTAT TGTTCATCGC 
GATATTAAAG GAGCAAATAT ATTTCTTACA TCTTCTGGAC TCATAAAACT GGGCGATTTT 
GGATGTTCGG TCAAGCTGAA GAATAATGCC CAGACTATGC CTGGAGAAGT TAACAGCACA 
CTTGGAACTG CAGCATATAT GGCTCCTGAA GTGATAACCC AAGCTAAGGG AGAGGGGCAT 
GGGCGTGCTG CAGACATATG GAGCCTTGGC TGTGTTTTAA TTGAGATGGT TACTGGCAAG 
AGACCTTGGC ATGAATATGA ACATAACTTC CAGATCATGT ACCGAGTCGG TATGGGACAC 
AAGCCCCCCA TTCCTGATCG GCTGAGCCCA GAGGGTAAAG ACTTCCTTTC TCATTGCCAG 
GAAAGTGATC CAAAAATGCG ATGGACAGCT AGCCAACTCT TGGACCATGC ATTTGTCAAG 
GTTTGCACAG ATGAAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k4 ( XM_012963756.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819210.1 Xenopus tropicalis mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X3, mRNA.

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CloneID	OXa36814
Clone ID Related Accession (Same CDS sequence)	XM_012963756.2
Accession Version	XM_012963756.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963756.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAAA CTGAAGATGC TACTGATGAC AATCTGGATT GTGAAGCTGA TGAGTTTTCA 
GATGAAGCAA GTGCAGAATC TCTCTATAAC ACCTTTCCGA CCAGCATTTC CCGACAGATG 
AAGCGAATGT CATCCAAGAA CCAGTGGAAT AATACAGGGA GGCCAGTTGG CCATTTCACG 
TTCAATGAAA AGATGAGTAG ACCAAATCCA TCACCTCACA AAGAGAGCAG CAAAACCACT 
GGAAATGTTG AGGATCATAA CTATAAGCAA GAGAAGAAGA ACAGAGCCAC TTTAAGAATG 
ACTGAGCGGG ATCAAAAAAA AAATGTGCAG TGCTCCTTTT TGCTTGACCC AGGAAGTGGG 
CCCCTGACAA AAAAATCTAT TCCAGATGTT GATCTTAATA AACCATATCT CAGTCTTGGC 
TGCAGTAGTT CCAAGCTACC TGTATCTGTA CCTGTTTCAT TAGCTAGGAC TACAAGGCAG 
AGCTCCAGGA CTGACTGCCC AGCTGACCGT CTCAAGTTCT TTGAGACACT GCGACTTTTG 
TTGAAACTAA CTTCCGTCTC TAAGAAAAAG GAGAGAGAAC AAAGGGGTCA GGAAAATACT 
TCTTCTATGG GGTATAACCA GTCTAATGAA CTTTTCTGGC TGGAACTGCA AGCTTGGCAT 
GCTGGTCGTA GTATTAATGA CCAAGACTTG TACCTATACA CAGCTCGACA GACAATTCCT 
GACATCATTA ATGAAATTTT AACTTTTAAG GTGAACTATG GGTCTTTATT TAGCGATGCT 
GATAACATCA ATGGGACATT TAGAGAAAGA CCATTTATTG TCTCACCAGG ATCTTTTGCT 
TTTGGCCACT CAAGTTATCA TGAACATATC CAGAAGCAAA AAATATCGTT TGAACAAGTA 
AAGCAGATTA TGGACCTGCT GGAATACATT GAGGCACTTT ACCCATCTTT GAATGCTCTT 
CAGAGGGATT ATGAAAAATA TGCTGCAAAA GATTTTCAGG CTAGAGTGCA GGCACTTTGC 
CTGTGGCTTA ATATAACCAA GGACTTGAAT CACAAATTGA GGATAATGGG ATCTGTTCTT 
GGTATCAAGA ACTTGTCAAA CATTGGCTGG CCAGTGTTTG AAATTTCTTC ACCATGTCCA 
TCAAATTGCC CAGAGGATGA ACATGACAAC ATAGAACATG AGGGACAAAT ACATGATGGA 
GCACCTAGAA TGTGTAGAGA GGAAAGAGAA GAAGAAGAGC TAACAATTAA CAATTTAGAT 
TTGAGACAAG ATTTTAACAG CAAGCTAACA GTTAATTTTT TTGAAGAAGA CTACTCCTCC 
TCAAACAAAC TAGATAAGAT TATGTCAGAG GATGATTTTA GCTGGGGAAA TCCAGAGTGC 
AATGATAGTG ATTGTAATAG GCATTGTTCA ACCTCCATTT ATAGACCTTT TGTTGACAAA 
GCTCTGAAAC AGATGGGACT GAGGAAACTT ATTCTGAGAC TGCATAAATT AATGCATGGT 
TCTCTTCAGA GAGCACGTAG AGCCTTAATG ATGGCTGATC GTACATTTGA TTTTTCAGAA 
TACCCTGACC TGATGTTATG TTCGGATTAC TTGCCTGACC TGTTGAAGCA ATCACGTAGT 
ACCGTGCAGA GCTCAGAACC TGCTTGTGAC CCTGTGTCAT GGGAGGAATT AAAGTCGATG 
GACCTGCCAT CCTTTCTACC TGCTTTTTTA GTCCTCTGTC GGGTCCTTCT AAATGTTATT 
CATGAATGTC TCAAACTGAG ACTGGAGCAA AGGCCAGCTG GGGAACCATC ACTCCTGAGC 
ATTAAGCAGT TAGTGAGAGA GTGCAAAGAA GTTCTGAAGG GCGGACTTCT CATGAAACAA 
TATTATCAGT TCATGCTCCG AGAAGTGTTG GATGACCTGC AGAAATTGGA TTGTGACATT 
GATTCATTTG AAGATGATCT TCACAAAATG TTAATGGTGT ACTTTGACTA TATGCGGAGC 
TGGATTCAAA TGCTTCAACA GTTACCTCAA GCTTCACACA GTCTAAAAAA CCTGCTAGAG 
GAAGAGTGGA ATTTCACCAA AGAAATCACT CCACTTATAC GTGGGGGGGA AGCACAGGCA 
GGGAAGCTTT TTTGCGATAT TGCAGGAATG CTGCTGAAAT CCACTGGGAA ATTTCTTGAC 
TCTGGTCTTC TGGACAGCTG TGACGAATTC TGGGCCAGCA CTGATGATAG TAGCATCTCG 
GATGAAATAA GGAGATCTGT TATTGAAACC AGCCGAGCAC TGAAAGAGCT GTTTCATGAA 
GCCAGAGAGA GAGCATCCAA AGCACTTGGC TTTGCAAAGA TGCTGAGAAA GGATTTGGAA 
ATAGCTGCAG AATTTACTTT TGCCTCTACT ATTCTTGAAC TACTTGCATT TTTAAAAGCC 
AAGGATTATG CAAAGGTGCT CATCCCTTCT GTGGAAAACC TTCATGTGTT TATACCAGGT 
AATCTCTCTA GAGAAAAGAT CATTGTTCAG CAATTACTTG GTACTGCAGC TGGCATAGAC 
TGTTCCAAAG ACTGTGAGGA CATGGCATAT GACCAGTTCC TGCTCATGAC GAAGCATATC 
GATAAAGATA AACCTCAGGA GGATTGGAAC AGTTGGGATG GATGCTGTAT TAAAAGCATG 
CCACCAGTGG AAACAGCAGA TACTCTAAGA AGTATGCAGA CTGACAACCT TCTGCTGGTG 
GTAATGCAGT CAGCCCACCT CCAGAGTCAA AGAAAGGCTT TCCAGCACTC CACAGAAGGG 
CTTTTATCTT TGCGATTGGA GCAGACATCA AGCCATCCAA TGATTGCAGA GGCTTTGTTC 
CAACTCAAGT ATCACAAAGA GGTGGTTCGT CTGATGTCTG GAGAGTTCAG ACAGAAAACT 
GGTGACAAAT ATATCAACTT TGCTAGAAAG TGGATGAACT TTGTGCTGAC AAAATGTGAA 
AGTGGTCGCG GCACCAGACC AAGGTGGGCA ACCCAAGGTT TTGACTATCT TCAAGCTATT 
GAACCAGTAT TCATTTCAGC TCTCCCAGAG GATGACTTTT TGAGTCTACA AGCTCTGATG 
AATGAATGCA TTGGCCATGT TATTGGGAAA CCCCACAGTC CTGTTACAGG TTTGTACATT 
GCTACTCATC GAAACAGCCC CCGTCCCGTG AAGGTGCCTC GATGCCACAG TGACCCACCA 
AATCCAAATC TGATCACTCC AACTCCTGAA GGCTTCAGGG GTTCCAGTGT TCCCGAAAAT 
GATCGTTTGG CCTCCGTAGC TTCAGAGTTA CAGTTCAGAT CCCTCAGTCG TCACTCTAGT 
CCAACAGAGG ACAGGGAAGA GCCGTCCTAT CCAAAAGGGG AGTTGTGGGG GTCAACTCGG 
CGAAGCTGGG AGCTTCAGAC ATTTATCAGT CAAACTCGAG ACATAGCTGC AAGACACCGG 
CCTATGAATG CTATACAGGA GTCGGTCAAA CAGTTTGAGG ATGAGCGCTA CCGGCAAATG 
AGAAATAAAA ATATTGTCGG CCAAGTCTGT GATACCCCAA AATCCTATGA CAATGTAATG 
CATGTTGGTC TTAGAAAAGT GACTTTCAAA TGGCAGAGAG GAAACAAAAT TGGGGAAGGG 
CAGTATGGCA AAGTGTACAC ATGTATTAGC ATTGACACAG GGGAACTGAT GGCAATGAAA 
GAAATACGTT TTCAGCCAAA TGATCACAAG ACTATAAAGG AAACTGCAGA TGAATTGAAA 
ATTTTTGAAG GAATCAAACA CCCAAATCTT GTTCGTTACT TTGGTGTCGA GCTCCACAGA 
GAAGAAATGT ATATTTTTAT GGAATACTGT GATGAAGGGA CTTTGGAGGA GGTATCTAGA 
CTGGGCCTGC AGGAGCATGT CATTAGACTT TATACAAAGC AGATAACCAT TGCTATAAAT 
GTCCTGCATG AGCATGGTAT TGTTCATCGC GATATTAAAG GAGCAAATAT ATTTCTTACA 
TCTTCTGGAC TCATAAAACT GGGCGATTTT GGATGTTCGG TCAAGCTGAA GAATAATGCC 
CAGACTATGC CTGGAGAAGT TAACAGCACA CTTGGAACTG CAGCATATAT GGCTCCTGAA 
GTGATAACCC AAGCTAAGGG AGAGGGGCAT GGGCGTGCTG CAGACATATG GAGCCTTGGC 
TGTGTTTTAA TTGAGATGGT TACTGGCAAG AGACCTTGGC ATGAATATGA ACATAACTTC 
CAGATCATGT ACCGAGTCGG TATGGGACAC AAGCCCCCCA TTCCTGATCG GCTGAGCCCA 
GAGGGTAAAG ACTTCCTTTC TCATTGCCAG GAAAGTGATC CAAAAATGCG ATGGACAGCT 
AGCCAACTCT TGGACCATGC ATTTGTCAAG GTTTGCACAG ATGAAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819210.1
CDS	94..4464
Translation	MKKTEDATDDNLDCEADEFSDEASAESLYNTFPTSISRQMKRMSSKNQWNNTGRPVGHFTFNEKMSRPNPSPHKESSKTTGNVEDHNYKQEKKNRATLRMTERDQKKNVQCSFLLDPGSGPLTKKSIPDVDLNKPYLSLGCSSSKLPVSVPVSLARTTRQSSRTDCPADRLKFFETLRLLLKLTSVSKKKEREQRGQENTSSMGYNQSNELFWLELQAWHAGRSINDQDLYLYTARQTIPDIINEILTFKVNYGSLFSDADNINGTFRERPFIVSPGSFAFGHSSYHEHIQKQKISFEQVKQIMDLLEYIEALYPSLNALQRDYEKYAAKDFQARVQALCLWLNITKDLNHKLRIMGSVLGIKNLSNIGWPVFEISSPCPSNCPEDEHDNIEHEGQIHDGAPRMCREEREEEELTINNLDLRQDFNSKLTVNFFEEDYSSSNKLDKIMSEDDFSWGNPECNDSDCNRHCSTSIYRPFVDKALKQMGLRKLILRLHKLMHGSLQRARRALMMADRTFDFSEYPDLMLCSDYLPDLLKQSRSTVQSSEPACDPVSWEELKSMDLPSFLPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLREVLDDLQKLDCDIDSFEDDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITPLIRGGEAQAGKLFCDIAGMLLKSTGKFLDSGLLDSCDEFWASTDDSSISDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFTFASTILELLAFLKAKDYAKVLIPSVENLHVFIPGNLSREKIIVQQLLGTAAGIDCSKDCEDMAYDQFLLMTKHIDKDKPQEDWNSWDGCCIKSMPPVETADTLRSMQTDNLLLVVMQSAHLQSQRKAFQHSTEGLLSLRLEQTSSHPMIAEALFQLKYHKEVVRLMSGEFRQKTGDKYINFARKWMNFVLTKCESGRGTRPRWATQGFDYLQAIEPVFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYIATHRNSPRPVKVPRCHSDPPNPNLITPTPEGFRGSSVPENDRLASVASELQFRSLSRHSSPTEDREEPSYPKGELWGSTRRSWELQTFISQTRDIAARHRPMNAIQESVKQFEDERYRQMRNKNIVGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISIDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYTKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITQAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYRVGMGHKPPIPDRLSPEGKDFLSHCQESDPKMRWTASQLLDHAFVKVCTDEE

Target ORF information:

RefSeq Version	XM_012963756.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963756.2

ORF Insert Sequence:

ATGAAAAAAA CTGAAGATGC TACTGATGAC AATCTGGATT GTGAAGCTGA TGAGTTTTCA 
GATGAAGCAA GTGCAGAATC TCTCTATAAC ACCTTTCCGA CCAGCATTTC CCGACAGATG 
AAGCGAATGT CATCCAAGAA CCAGTGGAAT AATACAGGGA GGCCAGTTGG CCATTTCACG 
TTCAATGAAA AGATGAGTAG ACCAAATCCA TCACCTCACA AAGAGAGCAG CAAAACCACT 
GGAAATGTTG AGGATCATAA CTATAAGCAA GAGAAGAAGA ACAGAGCCAC TTTAAGAATG 
ACTGAGCGGG ATCAAAAAAA AAATGTGCAG TGCTCCTTTT TGCTTGACCC AGGAAGTGGG 
CCCCTGACAA AAAAATCTAT TCCAGATGTT GATCTTAATA AACCATATCT CAGTCTTGGC 
TGCAGTAGTT CCAAGCTACC TGTATCTGTA CCTGTTTCAT TAGCTAGGAC TACAAGGCAG 
AGCTCCAGGA CTGACTGCCC AGCTGACCGT CTCAAGTTCT TTGAGACACT GCGACTTTTG 
TTGAAACTAA CTTCCGTCTC TAAGAAAAAG GAGAGAGAAC AAAGGGGTCA GGAAAATACT 
TCTTCTATGG GGTATAACCA GTCTAATGAA CTTTTCTGGC TGGAACTGCA AGCTTGGCAT 
GCTGGTCGTA GTATTAATGA CCAAGACTTG TACCTATACA CAGCTCGACA GACAATTCCT 
GACATCATTA ATGAAATTTT AACTTTTAAG GTGAACTATG GGTCTTTATT TAGCGATGCT 
GATAACATCA ATGGGACATT TAGAGAAAGA CCATTTATTG TCTCACCAGG ATCTTTTGCT 
TTTGGCCACT CAAGTTATCA TGAACATATC CAGAAGCAAA AAATATCGTT TGAACAAGTA 
AAGCAGATTA TGGACCTGCT GGAATACATT GAGGCACTTT ACCCATCTTT GAATGCTCTT 
CAGAGGGATT ATGAAAAATA TGCTGCAAAA GATTTTCAGG CTAGAGTGCA GGCACTTTGC 
CTGTGGCTTA ATATAACCAA GGACTTGAAT CACAAATTGA GGATAATGGG ATCTGTTCTT 
GGTATCAAGA ACTTGTCAAA CATTGGCTGG CCAGTGTTTG AAATTTCTTC ACCATGTCCA 
TCAAATTGCC CAGAGGATGA ACATGACAAC ATAGAACATG AGGGACAAAT ACATGATGGA 
GCACCTAGAA TGTGTAGAGA GGAAAGAGAA GAAGAAGAGC TAACAATTAA CAATTTAGAT 
TTGAGACAAG ATTTTAACAG CAAGCTAACA GTTAATTTTT TTGAAGAAGA CTACTCCTCC 
TCAAACAAAC TAGATAAGAT TATGTCAGAG GATGATTTTA GCTGGGGAAA TCCAGAGTGC 
AATGATAGTG ATTGTAATAG GCATTGTTCA ACCTCCATTT ATAGACCTTT TGTTGACAAA 
GCTCTGAAAC AGATGGGACT GAGGAAACTT ATTCTGAGAC TGCATAAATT AATGCATGGT 
TCTCTTCAGA GAGCACGTAG AGCCTTAATG ATGGCTGATC GTACATTTGA TTTTTCAGAA 
TACCCTGACC TGATGTTATG TTCGGATTAC TTGCCTGACC TGTTGAAGCA ATCACGTAGT 
ACCGTGCAGA GCTCAGAACC TGCTTGTGAC CCTGTGTCAT GGGAGGAATT AAAGTCGATG 
GACCTGCCAT CCTTTCTACC TGCTTTTTTA GTCCTCTGTC GGGTCCTTCT AAATGTTATT 
CATGAATGTC TCAAACTGAG ACTGGAGCAA AGGCCAGCTG GGGAACCATC ACTCCTGAGC 
ATTAAGCAGT TAGTGAGAGA GTGCAAAGAA GTTCTGAAGG GCGGACTTCT CATGAAACAA 
TATTATCAGT TCATGCTCCG AGAAGTGTTG GATGACCTGC AGAAATTGGA TTGTGACATT 
GATTCATTTG AAGATGATCT TCACAAAATG TTAATGGTGT ACTTTGACTA TATGCGGAGC 
TGGATTCAAA TGCTTCAACA GTTACCTCAA GCTTCACACA GTCTAAAAAA CCTGCTAGAG 
GAAGAGTGGA ATTTCACCAA AGAAATCACT CCACTTATAC GTGGGGGGGA AGCACAGGCA 
GGGAAGCTTT TTTGCGATAT TGCAGGAATG CTGCTGAAAT CCACTGGGAA ATTTCTTGAC 
TCTGGTCTTC TGGACAGCTG TGACGAATTC TGGGCCAGCA CTGATGATAG TAGCATCTCG 
GATGAAATAA GGAGATCTGT TATTGAAACC AGCCGAGCAC TGAAAGAGCT GTTTCATGAA 
GCCAGAGAGA GAGCATCCAA AGCACTTGGC TTTGCAAAGA TGCTGAGAAA GGATTTGGAA 
ATAGCTGCAG AATTTACTTT TGCCTCTACT ATTCTTGAAC TACTTGCATT TTTAAAAGCC 
AAGGATTATG CAAAGGTGCT CATCCCTTCT GTGGAAAACC TTCATGTGTT TATACCAGGT 
AATCTCTCTA GAGAAAAGAT CATTGTTCAG CAATTACTTG GTACTGCAGC TGGCATAGAC 
TGTTCCAAAG ACTGTGAGGA CATGGCATAT GACCAGTTCC TGCTCATGAC GAAGCATATC 
GATAAAGATA AACCTCAGGA GGATTGGAAC AGTTGGGATG GATGCTGTAT TAAAAGCATG 
CCACCAGTGG AAACAGCAGA TACTCTAAGA AGTATGCAGA CTGACAACCT TCTGCTGGTG 
GTAATGCAGT CAGCCCACCT CCAGAGTCAA AGAAAGGCTT TCCAGCACTC CACAGAAGGG 
CTTTTATCTT TGCGATTGGA GCAGACATCA AGCCATCCAA TGATTGCAGA GGCTTTGTTC 
CAACTCAAGT ATCACAAAGA GGTGGTTCGT CTGATGTCTG GAGAGTTCAG ACAGAAAACT 
GGTGACAAAT ATATCAACTT TGCTAGAAAG TGGATGAACT TTGTGCTGAC AAAATGTGAA 
AGTGGTCGCG GCACCAGACC AAGGTGGGCA ACCCAAGGTT TTGACTATCT TCAAGCTATT 
GAACCAGTAT TCATTTCAGC TCTCCCAGAG GATGACTTTT TGAGTCTACA AGCTCTGATG 
AATGAATGCA TTGGCCATGT TATTGGGAAA CCCCACAGTC CTGTTACAGG TTTGTACATT 
GCTACTCATC GAAACAGCCC CCGTCCCGTG AAGGTGCCTC GATGCCACAG TGACCCACCA 
AATCCAAATC TGATCACTCC AACTCCTGAA GGCTTCAGGG GTTCCAGTGT TCCCGAAAAT 
GATCGTTTGG CCTCCGTAGC TTCAGAGTTA CAGTTCAGAT CCCTCAGTCG TCACTCTAGT 
CCAACAGAGG ACAGGGAAGA GCCGTCCTAT CCAAAAGGGG AGTTGTGGGG GTCAACTCGG 
CGAAGCTGGG AGCTTCAGAC ATTTATCAGT CAAACTCGAG ACATAGCTGC AAGACACCGG 
CCTATGAATG CTATACAGGA GTCGGTCAAA CAGTTTGAGG ATGAGCGCTA CCGGCAAATG 
AGAAATAAAA ATATTGTCGG CCAAGTCTGT GATACCCCAA AATCCTATGA CAATGTAATG 
CATGTTGGTC TTAGAAAAGT GACTTTCAAA TGGCAGAGAG GAAACAAAAT TGGGGAAGGG 
CAGTATGGCA AAGTGTACAC ATGTATTAGC ATTGACACAG GGGAACTGAT GGCAATGAAA 
GAAATACGTT TTCAGCCAAA TGATCACAAG ACTATAAAGG AAACTGCAGA TGAATTGAAA 
ATTTTTGAAG GAATCAAACA CCCAAATCTT GTTCGTTACT TTGGTGTCGA GCTCCACAGA 
GAAGAAATGT ATATTTTTAT GGAATACTGT GATGAAGGGA CTTTGGAGGA GGTATCTAGA 
CTGGGCCTGC AGGAGCATGT CATTAGACTT TATACAAAGC AGATAACCAT TGCTATAAAT 
GTCCTGCATG AGCATGGTAT TGTTCATCGC GATATTAAAG GAGCAAATAT ATTTCTTACA 
TCTTCTGGAC TCATAAAACT GGGCGATTTT GGATGTTCGG TCAAGCTGAA GAATAATGCC 
CAGACTATGC CTGGAGAAGT TAACAGCACA CTTGGAACTG CAGCATATAT GGCTCCTGAA 
GTGATAACCC AAGCTAAGGG AGAGGGGCAT GGGCGTGCTG CAGACATATG GAGCCTTGGC 
TGTGTTTTAA TTGAGATGGT TACTGGCAAG AGACCTTGGC ATGAATATGA ACATAACTTC 
CAGATCATGT ACCGAGTCGG TATGGGACAC AAGCCCCCCA TTCCTGATCG GCTGAGCCCA 
GAGGGTAAAG ACTTCCTTTC TCATTGCCAG GAAAGTGATC CAAAAATGCG ATGGACAGCT 
AGCCAACTCT TGGACCATGC ATTTGTCAAG GTTTGCACAG ATGAAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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オンライン注文

map3k4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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