pde8a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	pde8a
Entrez Gene ID	100497070
Full Name	phosphodiesterase 8A
General protein information	Preferred Names phosphodiesterase 8A Names high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002939622.4	XP_002939668.2	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A isoform X2
XM_012959505.2	XP_012814959.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A isoform X1
XM_012959505.3	XP_012814959.2	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A isoform X1
XM_002939622.5	XP_002939668.3	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A isoform X2
XM_012959506.3	XP_012814960.2	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A isoform X3
XM_012959506.2	XP_012814960.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A isoform X3
XM_031899209.1	XP_031755069.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A isoform X4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PDE8A	XP_001163046.1
Homo sapiens (human)	PDE8A	NP_002596.1
Mus musculus (house mouse)	Pde8a	NP_032829.1
Caenorhabditis elegans (roundworm)	pde-6	NP_490787.1
	PDE8A	XP_001086066.1
Rattus norvegicus (Norway rat)	Pde8a	NP_942062.1
Danio rerio (zebrafish)	pde8a	NP_001074096.1
Canis lupus familiaris (dog)	PDE8A	XP_005618438.1
Xenopus tropicalis (tropical clawed frog)	pde8a	XP_002939668.2
Bos taurus (cattle)	PDE8A	NP_001178384.1
Gallus gallus (chicken)	PDE8A	XP_413834.2

Gene Ontology

The following pde8a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pde8a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa07231	XM_002939622.4 Latest version!	Xenopus tropicalis phosphodiesterase 8A (pde8a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$510.30 $729.00
OXa36757	XM_012959505.2 Latest version!	Xenopus tropicalis phosphodiesterase 8A (pde8a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa36758	XM_012959506.2 Latest version!	Xenopus tropicalis phosphodiesterase 8A (pde8a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa55137	XM_012959505.3 Latest version!	Xenopus tropicalis phosphodiesterase 8A (pde8a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa55138	XM_002939622.5 Latest version!	Xenopus tropicalis phosphodiesterase 8A (pde8a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa55139	XM_012959506.3 Latest version!	Xenopus tropicalis phosphodiesterase 8A (pde8a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa55140	XM_031899209.1 Latest version!	Xenopus tropicalis phosphodiesterase 8A (pde8a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pde8a ( XM_002939622.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939668.2 Xenopus tropicalis phosphodiesterase 8A (pde8a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa07231
Clone ID Related Accession (Same CDS sequence)	XM_002939622.4
Accession Version	XM_002939622.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002939622.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGTG CTCCAAGCAT CCACATCTCT GACAGCAGGG TGGTGTATCA CAGCGGCAAA 
GAGTCCGACG ATTCCAACTC CCCACACCAG ACCAACACAC TGGCTCAGCA AGGCAACTCC 
GTGCCTGGAT TATTCATTAA ATCCTCTCAC ACATCCACTT ATAAGGTGAG GGCTAATTCC 
TCTAGGAAAG GCAGGAGGGA GAACAGCCCG AGCATGGAGG CAGAGATAAA CAGCAGCACA 
AACAGCAGCA CAAACAGCAG CACATCCACC GCTGTAAAGG CAGCTGTAAG AGAAGTGCAG 
TTTGGTCCAA TGAAACTCCG CCAAGATCAA CTTCAGGTAC TTTTAGTATT TGCCAAAGAA 
GATAGCCAGA GTAATGGATT CTGTTGGGCG TGTGAGAAAG CAGGCTTTAA GTGTAACATT 
GCCAGGACGC CGGAGACTGC ACTGGAAAGC TTTTTAGATA AACATCACGA AATTATCATT 
ATTGATCATA GGCATTCACG ATATTTTGAT GCAGAAGCTC TATGCAGGTC CATTAGAGCT 
ACAAAACCCT CAGAAAACAC TGTCATAGTT GCTGTAGTTA GAAGACAAAG TGATCGGGAT 
GAAGCATCTA TTATGCCCCT AATATCTGCT GGATTTACCA GGAGATATGT AGAAAACTCC 
AATATTATGG CCTGTTACAA TGAGCTGATC CAGTTGGAAT TTGGAGAAGT ACTTGCACAA 
TTTAAACTAA GGGCTTGTAA TTCGTTATTT ACTACATTAG AAAAAAGTCA AGAAGCCATT 
GAAATTACAA GTGAGGATCA TATAATACAG TATGTGAATC CTGCTTTTGA AAGCACGATG 
GGTTATCAGA AGGACGAACT CATTGGGAAA GAATTAACTG AAGTGCCTAT TAATGAAAAA 
AAATCTGATT TCTTAGATAC CATTAATTGT TGCATTAAAA CGGGAAAGGA ATGGGAAGGT 
ATTTACTATG CCAAAAATAA AAATGGTGAC AATATCCAGC AAAATGTGAA GATAATTCCT 
GTTTTTGGAC ATCGGGGTAA AATCAGACAC TATGTGTCTA TAAGCCGTCC ATGCAGTGAC 
AACAACAAGG CAGAAAAACA ATGTGAGAGA GTTCAGGCCG AATCGCAAAC AGATATCCAG 
ACCTGTAGAC ACAAGGACAG GCGGAAAGGT TCCTTAGACG TCCGATCAAC CACCTCCAGA 
GGAAGCGATG GTAGTTCACA GAGACGGCAT TCTTCTATGG CCAGAATACA TTCTATGACC 
ATTGAAGCCC CTATCACAAA GGTAATAAAT ATAATAAATG CTGGACAAGA AAACAGCCCT 
TCGGCTGTGG CAGAAGCATT AGATCGAGTT CTAGAGATTC TGAGGACCAC AGAACTATAC 
TCTCCGCAGC TGGGGACGAA AGATGAAGAC CCTCACACCA ACGACCTGGT CGGAGGTTTG 
ATGACAGATG GTTTACGAAG ACTGTCAGGA AATGAGTACA TCTTATCAGC TAAACAGGTA 
CACCCTGTCA GTGGGCATAT GATCCTAGCT CTTTCCCTAA GCGATATCCC ACCTCGGATA 
GCACAAGCAA TGGATAATGA AGAAGACTGG GAGTTCGATA TCTTTGAATT AGAGGCTGCT 
ACACATAAAA GGCCCTTAGT GTATCTTGGC CTTAAAATGT TTGCTCGGTT TGGTGTTTGT 
CAGTTTCTAA ACTGCTCAGA AGCTACTTTG AGGTCTTGGC TACATGTTAT CGAGTCAAAC 
TACCATTCAA GCAACGCCTA TCATAATTCA ACGCATTCCG CTGATGTCTT ACATGCCACT 
GCTTATTTCC TCTGCAAAGA AAGAGTGAAG CAAAGCCTGG ATCAGATAGA TGAAGTTGCT 
GCTCTCATTG CTGCCACAGT CCATGATGTG GATCACCCTG GTCGGACAAA CTCGTTCCTT 
TGTAATGCAG GGAGTGAGCT GGCAATTCTG TACAACGACA CAGCAGTTTT AGAAAGTCAC 
CATGCAGCCC TAGCCTTCCA GCTGACCACT AGAGATGACA AGTGCAATAT ATTCAAAAAC 
ATGGAGAGGA ATGAATATCG CACTTTGCGG CAAGCAATCA TCGACATGGT CTTAGCAACT 
GAAATGACGA AGCACTTTGA ACATGTAAAC AAGTTTGTCA ACAGCATCAA TAAACCATTG 
GCAGCAGTGG AGGAGAATGG GGGCAGCATT GAGGAAGATG AGGCTGTTAA AAACATACTG 
ACATCTCCAG AAAACCGGAT CCTCATTAAA AGAATGCTTA TTAAGTGCGC AGACATTTCC 
AATCCGTGCC GGGCCCTCGA GCAGTGCATT GAGTGGGCTG GCCGGATATC AGAAGAGTAT 
TTTGCACAAA CAGATGAGGA GAAGAGAAAA GGTTTGCCTG TGGTGATGCC GGTGTTTGAC 
AGAAACACAT GTAGCATTCC GAAATCCCAG ATCTCCTTTG TTGATTACTT CATCACTGAT 
ATGTTCGATG CCTGGGATGC ATTTGCTGAT TTGCCCAACT TGATGCAACA TCTGGATAAT 
AACTTTAAAT ATTGGAAATC ACTAGACGAG AGACAGCTAA AGACTCTGCG ACCGCCCCCT 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939668.2
CDS	1054..3639
Translation	MGCAPSIHISDSRVVYHSGKESDDSNSPHQTNTLAQQGNSVPGLFIKSSHTSTYKVRANSSRKGRRENSPSMEAEINSSTNSSTNSSTSTAVKAAVREVQFGPMKLRQDQLQVLLVFAKEDSQSNGFCWACEKAGFKCNIARTPETALESFLDKHHEIIIIDHRHSRYFDAEALCRSIRATKPSENTVIVAVVRRQSDRDEASIMPLISAGFTRRYVENSNIMACYNELIQLEFGEVLAQFKLRACNSLFTTLEKSQEAIEITSEDHIIQYVNPAFESTMGYQKDELIGKELTEVPINEKKSDFLDTINCCIKTGKEWEGIYYAKNKNGDNIQQNVKIIPVFGHRGKIRHYVSISRPCSDNNKAEKQCERVQAESQTDIQTCRHKDRRKGSLDVRSTTSRGSDGSSQRRHSSMARIHSMTIEAPITKVINIINAGQENSPSAVAEALDRVLEILRTTELYSPQLGTKDEDPHTNDLVGGLMTDGLRRLSGNEYILSAKQVHPVSGHMILALSLSDIPPRIAQAMDNEEDWEFDIFELEAATHKRPLVYLGLKMFARFGVCQFLNCSEATLRSWLHVIESNYHSSNAYHNSTHSADVLHATAYFLCKERVKQSLDQIDEVAALIAATVHDVDHPGRTNSFLCNAGSELAILYNDTAVLESHHAALAFQLTTRDDKCNIFKNMERNEYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPLAAVEENGGSIEEDEAVKNILTSPENRILIKRMLIKCADISNPCRALEQCIEWAGRISEEYFAQTDEEKRKGLPVVMPVFDRNTCSIPKSQISFVDYFITDMFDAWDAFADLPNLMQHLDNNFKYWKSLDERQLKTLRPPPE

Target ORF information:

RefSeq Version	XM_002939622.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 8A (pde8a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939622.4

ORF Insert Sequence:

ATGGGCTGTG CTCCAAGCAT CCACATCTCT GACAGCAGGG TGGTGTATCA CAGCGGCAAA 
GAGTCCGACG ATTCCAACTC CCCACACCAG ACCAACACAC TGGCTCAGCA AGGCAACTCC 
GTGCCTGGAT TATTCATTAA ATCCTCTCAC ACATCCACTT ATAAGGTGAG GGCTAATTCC 
TCTAGGAAAG GCAGGAGGGA GAACAGCCCG AGCATGGAGG CAGAGATAAA CAGCAGCACA 
AACAGCAGCA CAAACAGCAG CACATCCACC GCTGTAAAGG CAGCTGTAAG AGAAGTGCAG 
TTTGGTCCAA TGAAACTCCG CCAAGATCAA CTTCAGGTAC TTTTAGTATT TGCCAAAGAA 
GATAGCCAGA GTAATGGATT CTGTTGGGCG TGTGAGAAAG CAGGCTTTAA GTGTAACATT 
GCCAGGACGC CGGAGACTGC ACTGGAAAGC TTTTTAGATA AACATCACGA AATTATCATT 
ATTGATCATA GGCATTCACG ATATTTTGAT GCAGAAGCTC TATGCAGGTC CATTAGAGCT 
ACAAAACCCT CAGAAAACAC TGTCATAGTT GCTGTAGTTA GAAGACAAAG TGATCGGGAT 
GAAGCATCTA TTATGCCCCT AATATCTGCT GGATTTACCA GGAGATATGT AGAAAACTCC 
AATATTATGG CCTGTTACAA TGAGCTGATC CAGTTGGAAT TTGGAGAAGT ACTTGCACAA 
TTTAAACTAA GGGCTTGTAA TTCGTTATTT ACTACATTAG AAAAAAGTCA AGAAGCCATT 
GAAATTACAA GTGAGGATCA TATAATACAG TATGTGAATC CTGCTTTTGA AAGCACGATG 
GGTTATCAGA AGGACGAACT CATTGGGAAA GAATTAACTG AAGTGCCTAT TAATGAAAAA 
AAATCTGATT TCTTAGATAC CATTAATTGT TGCATTAAAA CGGGAAAGGA ATGGGAAGGT 
ATTTACTATG CCAAAAATAA AAATGGTGAC AATATCCAGC AAAATGTGAA GATAATTCCT 
GTTTTTGGAC ATCGGGGTAA AATCAGACAC TATGTGTCTA TAAGCCGTCC ATGCAGTGAC 
AACAACAAGG CAGAAAAACA ATGTGAGAGA GTTCAGGCCG AATCGCAAAC AGATATCCAG 
ACCTGTAGAC ACAAGGACAG GCGGAAAGGT TCCTTAGACG TCCGATCAAC CACCTCCAGA 
GGAAGCGATG GTAGTTCACA GAGACGGCAT TCTTCTATGG CCAGAATACA TTCTATGACC 
ATTGAAGCCC CTATCACAAA GGTAATAAAT ATAATAAATG CTGGACAAGA AAACAGCCCT 
TCGGCTGTGG CAGAAGCATT AGATCGAGTT CTAGAGATTC TGAGGACCAC AGAACTATAC 
TCTCCGCAGC TGGGGACGAA AGATGAAGAC CCTCACACCA ACGACCTGGT CGGAGGTTTG 
ATGACAGATG GTTTACGAAG ACTGTCAGGA AATGAGTACA TCTTATCAGC TAAACAGGTA 
CACCCTGTCA GTGGGCATAT GATCCTAGCT CTTTCCCTAA GCGATATCCC ACCTCGGATA 
GCACAAGCAA TGGATAATGA AGAAGACTGG GAGTTCGATA TCTTTGAATT AGAGGCTGCT 
ACACATAAAA GGCCCTTAGT GTATCTTGGC CTTAAAATGT TTGCTCGGTT TGGTGTTTGT 
CAGTTTCTAA ACTGCTCAGA AGCTACTTTG AGGTCTTGGC TACATGTTAT CGAGTCAAAC 
TACCATTCAA GCAACGCCTA TCATAATTCA ACGCATTCCG CTGATGTCTT ACATGCCACT 
GCTTATTTCC TCTGCAAAGA AAGAGTGAAG CAAAGCCTGG ATCAGATAGA TGAAGTTGCT 
GCTCTCATTG CTGCCACAGT CCATGATGTG GATCACCCTG GTCGGACAAA CTCGTTCCTT 
TGTAATGCAG GGAGTGAGCT GGCAATTCTG TACAACGACA CAGCAGTTTT AGAAAGTCAC 
CATGCAGCCC TAGCCTTCCA GCTGACCACT AGAGATGACA AGTGCAATAT ATTCAAAAAC 
ATGGAGAGGA ATGAATATCG CACTTTGCGG CAAGCAATCA TCGACATGGT CTTAGCAACT 
GAAATGACGA AGCACTTTGA ACATGTAAAC AAGTTTGTCA ACAGCATCAA TAAACCATTG 
GCAGCAGTGG AGGAGAATGG GGGCAGCATT GAGGAAGATG AGGCTGTTAA AAACATACTG 
ACATCTCCAG AAAACCGGAT CCTCATTAAA AGAATGCTTA TTAAGTGCGC AGACATTTCC 
AATCCGTGCC GGGCCCTCGA GCAGTGCATT GAGTGGGCTG GCCGGATATC AGAAGAGTAT 
TTTGCACAAA CAGATGAGGA GAAGAGAAAA GGTTTGCCTG TGGTGATGCC GGTGTTTGAC 
AGAAACACAT GTAGCATTCC GAAATCCCAG ATCTCCTTTG TTGATTACTT CATCACTGAT 
ATGTTCGATG CCTGGGATGC ATTTGCTGAT TTGCCCAACT TGATGCAACA TCTGGATAAT 
AACTTTAAAT ATTGGAAATC ACTAGACGAG AGACAGCTAA AGACTCTGCG ACCGCCCCCT 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde8a ( XM_012959505.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814959.1 Xenopus tropicalis phosphodiesterase 8A (pde8a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa36757
Clone ID Related Accession (Same CDS sequence)	XM_012959505.2
Accession Version	XM_012959505.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959505.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGTG CTCCAAGCAT CCACATCTCT GACAGCAGGG TGGTGTATCA CAGCGGCAAA 
GAGTCCGACG ATTCCAACTC CCCACACCAG ACCAACACAC TGGCTCAGCA AGGCAACTCC 
GTGCCTGGAT TATTCATTAA ATCCTCTCAC ACATCCACTT ATAAGGTGAG GGCTAATTCC 
TCTAGGAAAG GCAGGAGGGA GAACAGCCCG AGCATGGAGG CAGAGATAAA CAGCAGCACA 
AACAGCAGCA CAAACAGCAG CACATCCACC GCTGTAAAGG CAGCTGTAAG AGAAGTGCAG 
TTTGGTCCAA TGAAACTCCG CCAAGATCAA CTTCAGGTAC TTTTAGTATT TGCCAAAGAA 
GATAGCCAGA GTAATGGATT CTGTTGGGCG TGTGAGAAAG CAGGCTTTAA GTGTAACATT 
GCCAGGACGC CGGAGACTGC ACTGGAAAGC TTTTTAGATA AACATCACGA AATTATCATT 
ATTGATCATA GGCATTCACG ATATTTTGAT GCAGAAGCTC TATGCAGGTC CATTAGAGCT 
ACAAAACCCT CAGAAAACAC TGTCATAGTT GCTGTAGTTA GAAGACAAAG TGATCGGGAT 
GAAGCATCTA TTATGCCCCT AATATCTGCT GGATTTACCA GGAGATATGT AGAAAACTCC 
AATATTATGG CCTGTTACAA TGAGCTGATC CAGTTGGAAT TTGGAGAAGT ACTTGCACAA 
TTTAAACTAA GGGCTTGTAA TTCGTTATTT ACTACATTAG AAAAAAGTCA AGAAGCCATT 
GAAATTACAA GTGAGGATCA TATAATACAG TATGTGAATC CTGCTTTTGA AAGCACGATG 
GGTTATCAGA AGGACGAACT CATTGGGAAA GAATTAACTG AAGTGCCTAT TAATGAAAAA 
AAATCTGATT TCTTAGATAC CATTAATTGT TGCATTAAAA CGGGAAAGGA ATGGGAAGGT 
ATTTACTATG CCAAAAATAA AAATGGTGAC AATATCCAGC AAAATGTGAA GATAATTCCT 
GTTTTTGGAC ATCGGGGTAA AATCAGACAC TATGTGTCTA TAAGCCGTCC ATGCAGTGAC 
AACAACAAGG CAGAAAAACA ATGTGAGAGA GTTCAGGCCG AATCGCAAAC AGATATCCAG 
ACCTGTAGAC ACAAGGACAG GCGGAAAGGT TCCTTAGACG TCCGATCAAC CACCTCCAGA 
GGAAGCGATG GTCCAGCTGA ATTTTCACAC GGTGCTCTAC CTGGTAGTTC ACAGAGACGG 
CATTCTTCTA TGGCCAGAAT ACATTCTATG ACCATTGAAG CCCCTATCAC AAAGGTAATA 
AATATAATAA ATGCTGGACA AGAAAACAGC CCTTCGGCTG TGGCAGAAGC ATTAGATCGA 
GTTCTAGAGA TTCTGAGGAC CACAGAACTA TACTCTCCGC AGCTGGGGAC GAAAGATGAA 
GACCCTCACA CCAACGACCT GGTCGGAGGT TTGATGACAG ATGGTTTACG AAGACTGTCA 
GGAAATGAGT ACATCTTATC AGCTAAACAG GTACACCCTG TCAGTGGGCA TATGATCCTA 
GCTCTTTCCC TAAGCGATAT CCCACCTCGG ATAGCACAAG CAATGGATAA TGAAGAAGAC 
TGGGAGTTCG ATATCTTTGA ATTAGAGGCT GCTACACATA AAAGGCCCTT AGTGTATCTT 
GGCCTTAAAA TGTTTGCTCG GTTTGGTGTT TGTCAGTTTC TAAACTGCTC AGAAGCTACT 
TTGAGGTCTT GGCTACATGT TATCGAGTCA AACTACCATT CAAGCAACGC CTATCATAAT 
TCAACGCATT CCGCTGATGT CTTACATGCC ACTGCTTATT TCCTCTGCAA AGAAAGAGTG 
AAGCAAAGCC TGGATCAGAT AGATGAAGTT GCTGCTCTCA TTGCTGCCAC AGTCCATGAT 
GTGGATCACC CTGGTCGGAC AAACTCGTTC CTTTGTAATG CAGGGAGTGA GCTGGCAATT 
CTGTACAACG ACACAGCAGT TTTAGAAAGT CACCATGCAG CCCTAGCCTT CCAGCTGACC 
ACTAGAGATG ACAAGTGCAA TATATTCAAA AACATGGAGA GGAATGAATA TCGCACTTTG 
CGGCAAGCAA TCATCGACAT GGTCTTAGCA ACTGAAATGA CGAAGCACTT TGAACATGTA 
AACAAGTTTG TCAACAGCAT CAATAAACCA TTGGCAGCAG TGGAGGAGAA TGGGGGCAGC 
ATTGAGGAAG ATGAGGCTGT TAAAAACATA CTGACATCTC CAGAAAACCG GATCCTCATT 
AAAAGAATGC TTATTAAGTG CGCAGACATT TCCAATCCGT GCCGGGCCCT CGAGCAGTGC 
ATTGAGTGGG CTGGCCGGAT ATCAGAAGAG TATTTTGCAC AAACAGATGA GGAGAAGAGA 
AAAGGTTTGC CTGTGGTGAT GCCGGTGTTT GACAGAAACA CATGTAGCAT TCCGAAATCC 
CAGATCTCCT TTGTTGATTA CTTCATCACT GATATGTTCG ATGCCTGGGA TGCATTTGCT 
GATTTGCCCA ACTTGATGCA ACATCTGGAT AATAACTTTA AATATTGGAA ATCACTAGAC 
GAGAGACAGC TAAAGACTCT GCGACCGCCC CCTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814959.1
CDS	1055..3673
Translation	MGCAPSIHISDSRVVYHSGKESDDSNSPHQTNTLAQQGNSVPGLFIKSSHTSTYKVRANSSRKGRRENSPSMEAEINSSTNSSTNSSTSTAVKAAVREVQFGPMKLRQDQLQVLLVFAKEDSQSNGFCWACEKAGFKCNIARTPETALESFLDKHHEIIIIDHRHSRYFDAEALCRSIRATKPSENTVIVAVVRRQSDRDEASIMPLISAGFTRRYVENSNIMACYNELIQLEFGEVLAQFKLRACNSLFTTLEKSQEAIEITSEDHIIQYVNPAFESTMGYQKDELIGKELTEVPINEKKSDFLDTINCCIKTGKEWEGIYYAKNKNGDNIQQNVKIIPVFGHRGKIRHYVSISRPCSDNNKAEKQCERVQAESQTDIQTCRHKDRRKGSLDVRSTTSRGSDGPAEFSHGALPGSSQRRHSSMARIHSMTIEAPITKVINIINAGQENSPSAVAEALDRVLEILRTTELYSPQLGTKDEDPHTNDLVGGLMTDGLRRLSGNEYILSAKQVHPVSGHMILALSLSDIPPRIAQAMDNEEDWEFDIFELEAATHKRPLVYLGLKMFARFGVCQFLNCSEATLRSWLHVIESNYHSSNAYHNSTHSADVLHATAYFLCKERVKQSLDQIDEVAALIAATVHDVDHPGRTNSFLCNAGSELAILYNDTAVLESHHAALAFQLTTRDDKCNIFKNMERNEYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPLAAVEENGGSIEEDEAVKNILTSPENRILIKRMLIKCADISNPCRALEQCIEWAGRISEEYFAQTDEEKRKGLPVVMPVFDRNTCSIPKSQISFVDYFITDMFDAWDAFADLPNLMQHLDNNFKYWKSLDERQLKTLRPPPE

Target ORF information:

RefSeq Version	XM_012959505.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 8A (pde8a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959505.2

ORF Insert Sequence:

ATGGGCTGTG CTCCAAGCAT CCACATCTCT GACAGCAGGG TGGTGTATCA CAGCGGCAAA 
GAGTCCGACG ATTCCAACTC CCCACACCAG ACCAACACAC TGGCTCAGCA AGGCAACTCC 
GTGCCTGGAT TATTCATTAA ATCCTCTCAC ACATCCACTT ATAAGGTGAG GGCTAATTCC 
TCTAGGAAAG GCAGGAGGGA GAACAGCCCG AGCATGGAGG CAGAGATAAA CAGCAGCACA 
AACAGCAGCA CAAACAGCAG CACATCCACC GCTGTAAAGG CAGCTGTAAG AGAAGTGCAG 
TTTGGTCCAA TGAAACTCCG CCAAGATCAA CTTCAGGTAC TTTTAGTATT TGCCAAAGAA 
GATAGCCAGA GTAATGGATT CTGTTGGGCG TGTGAGAAAG CAGGCTTTAA GTGTAACATT 
GCCAGGACGC CGGAGACTGC ACTGGAAAGC TTTTTAGATA AACATCACGA AATTATCATT 
ATTGATCATA GGCATTCACG ATATTTTGAT GCAGAAGCTC TATGCAGGTC CATTAGAGCT 
ACAAAACCCT CAGAAAACAC TGTCATAGTT GCTGTAGTTA GAAGACAAAG TGATCGGGAT 
GAAGCATCTA TTATGCCCCT AATATCTGCT GGATTTACCA GGAGATATGT AGAAAACTCC 
AATATTATGG CCTGTTACAA TGAGCTGATC CAGTTGGAAT TTGGAGAAGT ACTTGCACAA 
TTTAAACTAA GGGCTTGTAA TTCGTTATTT ACTACATTAG AAAAAAGTCA AGAAGCCATT 
GAAATTACAA GTGAGGATCA TATAATACAG TATGTGAATC CTGCTTTTGA AAGCACGATG 
GGTTATCAGA AGGACGAACT CATTGGGAAA GAATTAACTG AAGTGCCTAT TAATGAAAAA 
AAATCTGATT TCTTAGATAC CATTAATTGT TGCATTAAAA CGGGAAAGGA ATGGGAAGGT 
ATTTACTATG CCAAAAATAA AAATGGTGAC AATATCCAGC AAAATGTGAA GATAATTCCT 
GTTTTTGGAC ATCGGGGTAA AATCAGACAC TATGTGTCTA TAAGCCGTCC ATGCAGTGAC 
AACAACAAGG CAGAAAAACA ATGTGAGAGA GTTCAGGCCG AATCGCAAAC AGATATCCAG 
ACCTGTAGAC ACAAGGACAG GCGGAAAGGT TCCTTAGACG TCCGATCAAC CACCTCCAGA 
GGAAGCGATG GTCCAGCTGA ATTTTCACAC GGTGCTCTAC CTGGTAGTTC ACAGAGACGG 
CATTCTTCTA TGGCCAGAAT ACATTCTATG ACCATTGAAG CCCCTATCAC AAAGGTAATA 
AATATAATAA ATGCTGGACA AGAAAACAGC CCTTCGGCTG TGGCAGAAGC ATTAGATCGA 
GTTCTAGAGA TTCTGAGGAC CACAGAACTA TACTCTCCGC AGCTGGGGAC GAAAGATGAA 
GACCCTCACA CCAACGACCT GGTCGGAGGT TTGATGACAG ATGGTTTACG AAGACTGTCA 
GGAAATGAGT ACATCTTATC AGCTAAACAG GTACACCCTG TCAGTGGGCA TATGATCCTA 
GCTCTTTCCC TAAGCGATAT CCCACCTCGG ATAGCACAAG CAATGGATAA TGAAGAAGAC 
TGGGAGTTCG ATATCTTTGA ATTAGAGGCT GCTACACATA AAAGGCCCTT AGTGTATCTT 
GGCCTTAAAA TGTTTGCTCG GTTTGGTGTT TGTCAGTTTC TAAACTGCTC AGAAGCTACT 
TTGAGGTCTT GGCTACATGT TATCGAGTCA AACTACCATT CAAGCAACGC CTATCATAAT 
TCAACGCATT CCGCTGATGT CTTACATGCC ACTGCTTATT TCCTCTGCAA AGAAAGAGTG 
AAGCAAAGCC TGGATCAGAT AGATGAAGTT GCTGCTCTCA TTGCTGCCAC AGTCCATGAT 
GTGGATCACC CTGGTCGGAC AAACTCGTTC CTTTGTAATG CAGGGAGTGA GCTGGCAATT 
CTGTACAACG ACACAGCAGT TTTAGAAAGT CACCATGCAG CCCTAGCCTT CCAGCTGACC 
ACTAGAGATG ACAAGTGCAA TATATTCAAA AACATGGAGA GGAATGAATA TCGCACTTTG 
CGGCAAGCAA TCATCGACAT GGTCTTAGCA ACTGAAATGA CGAAGCACTT TGAACATGTA 
AACAAGTTTG TCAACAGCAT CAATAAACCA TTGGCAGCAG TGGAGGAGAA TGGGGGCAGC 
ATTGAGGAAG ATGAGGCTGT TAAAAACATA CTGACATCTC CAGAAAACCG GATCCTCATT 
AAAAGAATGC TTATTAAGTG CGCAGACATT TCCAATCCGT GCCGGGCCCT CGAGCAGTGC 
ATTGAGTGGG CTGGCCGGAT ATCAGAAGAG TATTTTGCAC AAACAGATGA GGAGAAGAGA 
AAAGGTTTGC CTGTGGTGAT GCCGGTGTTT GACAGAAACA CATGTAGCAT TCCGAAATCC 
CAGATCTCCT TTGTTGATTA CTTCATCACT GATATGTTCG ATGCCTGGGA TGCATTTGCT 
GATTTGCCCA ACTTGATGCA ACATCTGGAT AATAACTTTA AATATTGGAA ATCACTAGAC 
GAGAGACAGC TAAAGACTCT GCGACCGCCC CCTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde8a ( XM_012959506.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814960.1 Xenopus tropicalis phosphodiesterase 8A (pde8a), transcript variant X3, mRNA.

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CloneID	OXa36758
Clone ID Related Accession (Same CDS sequence)	XM_012959506.2
Accession Version	XM_012959506.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959506.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGTG CTCCAAGCAT CCACATCTCT GACAGCAGGG TGGTGTATCA CAGCGGCAAA 
GAGTCCGACG ATTCCAACTC CCCACACCAG ACCAACACAC TGGCTCAGCA AGGCAACTCC 
GTGCCTGGAT TATTCATTAA ATCCTCTCAC ACATCCACTT ATAAGGCAGC TGTAAGAGAA 
GTGCAGTTTG GTCCAATGAA ACTCCGCCAA GATCAACTTC AGGTACTTTT AGTATTTGCC 
AAAGAAGATA GCCAGAGTAA TGGATTCTGT TGGGCGTGTG AGAAAGCAGG CTTTAAGTGT 
AACATTGCCA GGACGCCGGA GACTGCACTG GAAAGCTTTT TAGATAAACA TCACGAAATT 
ATCATTATTG ATCATAGGCA TTCACGATAT TTTGATGCAG AAGCTCTATG CAGGTCCATT 
AGAGCTACAA AACCCTCAGA AAACACTGTC ATAGTTGCTG TAGTTAGAAG ACAAAGTGAT 
CGGGATGAAG CATCTATTAT GCCCCTAATA TCTGCTGGAT TTACCAGGAG ATATGTAGAA 
AACTCCAATA TTATGGCCTG TTACAATGAG CTGATCCAGT TGGAATTTGG AGAAGTACTT 
GCACAATTTA AACTAAGGGC TTGTAATTCG TTATTTACTA CATTAGAAAA AAGTCAAGAA 
GCCATTGAAA TTACAAGTGA GGATCATATA ATACAGTATG TGAATCCTGC TTTTGAAAGC 
ACGATGGGTT ATCAGAAGGA CGAACTCATT GGGAAAGAAT TAACTGAAGT GCCTATTAAT 
GAAAAAAAAT CTGATTTCTT AGATACCATT AATTGTTGCA TTAAAACGGG AAAGGAATGG 
GAAGGTATTT ACTATGCCAA AAATAAAAAT GGTGACAATA TCCAGCAAAA TGTGAAGATA 
ATTCCTGTTT TTGGACATCG GGGTAAAATC AGACACTATG TGTCTATAAG CCGTCCATGC 
AGTGACAACA ACAAGGCAGA AAAACAATGT GAGAGAGTTC AGGCCGAATC GCAAACAGAT 
ATCCAGACCT GTAGACACAA GGACAGGCGG AAAGGTTCCT TAGACGTCCG ATCAACCACC 
TCCAGAGGAA GCGATGGTCC AGCTGAATTT TCACACGGTG CTCTACCTGG TAGTTCACAG 
AGACGGCATT CTTCTATGGC CAGAATACAT TCTATGACCA TTGAAGCCCC TATCACAAAG 
GTAATAAATA TAATAAATGC TGGACAAGAA AACAGCCCTT CGGCTGTGGC AGAAGCATTA 
GATCGAGTTC TAGAGATTCT GAGGACCACA GAACTATACT CTCCGCAGCT GGGGACGAAA 
GATGAAGACC CTCACACCAA CGACCTGGTC GGAGGTTTGA TGACAGATGG TTTACGAAGA 
CTGTCAGGAA ATGAGTACAT CTTATCAGCT AAACAGGTAC ACCCTGTCAG TGGGCATATG 
ATCCTAGCTC TTTCCCTAAG CGATATCCCA CCTCGGATAG CACAAGCAAT GGATAATGAA 
GAAGACTGGG AGTTCGATAT CTTTGAATTA GAGGCTGCTA CACATAAAAG GCCCTTAGTG 
TATCTTGGCC TTAAAATGTT TGCTCGGTTT GGTGTTTGTC AGTTTCTAAA CTGCTCAGAA 
GCTACTTTGA GGTCTTGGCT ACATGTTATC GAGTCAAACT ACCATTCAAG CAACGCCTAT 
CATAATTCAA CGCATTCCGC TGATGTCTTA CATGCCACTG CTTATTTCCT CTGCAAAGAA 
AGAGTGAAGC AAAGCCTGGA TCAGATAGAT GAAGTTGCTG CTCTCATTGC TGCCACAGTC 
CATGATGTGG ATCACCCTGG TCGGACAAAC TCGTTCCTTT GTAATGCAGG GAGTGAGCTG 
GCAATTCTGT ACAACGACAC AGCAGTTTTA GAAAGTCACC ATGCAGCCCT AGCCTTCCAG 
CTGACCACTA GAGATGACAA GTGCAATATA TTCAAAAACA TGGAGAGGAA TGAATATCGC 
ACTTTGCGGC AAGCAATCAT CGACATGGTC TTAGCAACTG AAATGACGAA GCACTTTGAA 
CATGTAAACA AGTTTGTCAA CAGCATCAAT AAACCATTGG CAGCAGTGGA GGAGAATGGG 
GGCAGCATTG AGGAAGATGA GGCTGTTAAA AACATACTGA CATCTCCAGA AAACCGGATC 
CTCATTAAAA GAATGCTTAT TAAGTGCGCA GACATTTCCA ATCCGTGCCG GGCCCTCGAG 
CAGTGCATTG AGTGGGCTGG CCGGATATCA GAAGAGTATT TTGCACAAAC AGATGAGGAG 
AAGAGAAAAG GTTTGCCTGT GGTGATGCCG GTGTTTGACA GAAACACATG TAGCATTCCG 
AAATCCCAGA TCTCCTTTGT TGATTACTTC ATCACTGATA TGTTCGATGC CTGGGATGCA 
TTTGCTGATT TGCCCAACTT GATGCAACAT CTGGATAATA ACTTTAAATA TTGGAAATCA 
CTAGACGAGA GACAGCTAAA GACTCTGCGA CCGCCCCCTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814960.1
CDS	1055..3559
Translation	MGCAPSIHISDSRVVYHSGKESDDSNSPHQTNTLAQQGNSVPGLFIKSSHTSTYKAAVREVQFGPMKLRQDQLQVLLVFAKEDSQSNGFCWACEKAGFKCNIARTPETALESFLDKHHEIIIIDHRHSRYFDAEALCRSIRATKPSENTVIVAVVRRQSDRDEASIMPLISAGFTRRYVENSNIMACYNELIQLEFGEVLAQFKLRACNSLFTTLEKSQEAIEITSEDHIIQYVNPAFESTMGYQKDELIGKELTEVPINEKKSDFLDTINCCIKTGKEWEGIYYAKNKNGDNIQQNVKIIPVFGHRGKIRHYVSISRPCSDNNKAEKQCERVQAESQTDIQTCRHKDRRKGSLDVRSTTSRGSDGPAEFSHGALPGSSQRRHSSMARIHSMTIEAPITKVINIINAGQENSPSAVAEALDRVLEILRTTELYSPQLGTKDEDPHTNDLVGGLMTDGLRRLSGNEYILSAKQVHPVSGHMILALSLSDIPPRIAQAMDNEEDWEFDIFELEAATHKRPLVYLGLKMFARFGVCQFLNCSEATLRSWLHVIESNYHSSNAYHNSTHSADVLHATAYFLCKERVKQSLDQIDEVAALIAATVHDVDHPGRTNSFLCNAGSELAILYNDTAVLESHHAALAFQLTTRDDKCNIFKNMERNEYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPLAAVEENGGSIEEDEAVKNILTSPENRILIKRMLIKCADISNPCRALEQCIEWAGRISEEYFAQTDEEKRKGLPVVMPVFDRNTCSIPKSQISFVDYFITDMFDAWDAFADLPNLMQHLDNNFKYWKSLDERQLKTLRPPPE

Target ORF information:

RefSeq Version	XM_012959506.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 8A (pde8a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959506.2

ORF Insert Sequence:

ATGGGCTGTG CTCCAAGCAT CCACATCTCT GACAGCAGGG TGGTGTATCA CAGCGGCAAA 
GAGTCCGACG ATTCCAACTC CCCACACCAG ACCAACACAC TGGCTCAGCA AGGCAACTCC 
GTGCCTGGAT TATTCATTAA ATCCTCTCAC ACATCCACTT ATAAGGCAGC TGTAAGAGAA 
GTGCAGTTTG GTCCAATGAA ACTCCGCCAA GATCAACTTC AGGTACTTTT AGTATTTGCC 
AAAGAAGATA GCCAGAGTAA TGGATTCTGT TGGGCGTGTG AGAAAGCAGG CTTTAAGTGT 
AACATTGCCA GGACGCCGGA GACTGCACTG GAAAGCTTTT TAGATAAACA TCACGAAATT 
ATCATTATTG ATCATAGGCA TTCACGATAT TTTGATGCAG AAGCTCTATG CAGGTCCATT 
AGAGCTACAA AACCCTCAGA AAACACTGTC ATAGTTGCTG TAGTTAGAAG ACAAAGTGAT 
CGGGATGAAG CATCTATTAT GCCCCTAATA TCTGCTGGAT TTACCAGGAG ATATGTAGAA 
AACTCCAATA TTATGGCCTG TTACAATGAG CTGATCCAGT TGGAATTTGG AGAAGTACTT 
GCACAATTTA AACTAAGGGC TTGTAATTCG TTATTTACTA CATTAGAAAA AAGTCAAGAA 
GCCATTGAAA TTACAAGTGA GGATCATATA ATACAGTATG TGAATCCTGC TTTTGAAAGC 
ACGATGGGTT ATCAGAAGGA CGAACTCATT GGGAAAGAAT TAACTGAAGT GCCTATTAAT 
GAAAAAAAAT CTGATTTCTT AGATACCATT AATTGTTGCA TTAAAACGGG AAAGGAATGG 
GAAGGTATTT ACTATGCCAA AAATAAAAAT GGTGACAATA TCCAGCAAAA TGTGAAGATA 
ATTCCTGTTT TTGGACATCG GGGTAAAATC AGACACTATG TGTCTATAAG CCGTCCATGC 
AGTGACAACA ACAAGGCAGA AAAACAATGT GAGAGAGTTC AGGCCGAATC GCAAACAGAT 
ATCCAGACCT GTAGACACAA GGACAGGCGG AAAGGTTCCT TAGACGTCCG ATCAACCACC 
TCCAGAGGAA GCGATGGTCC AGCTGAATTT TCACACGGTG CTCTACCTGG TAGTTCACAG 
AGACGGCATT CTTCTATGGC CAGAATACAT TCTATGACCA TTGAAGCCCC TATCACAAAG 
GTAATAAATA TAATAAATGC TGGACAAGAA AACAGCCCTT CGGCTGTGGC AGAAGCATTA 
GATCGAGTTC TAGAGATTCT GAGGACCACA GAACTATACT CTCCGCAGCT GGGGACGAAA 
GATGAAGACC CTCACACCAA CGACCTGGTC GGAGGTTTGA TGACAGATGG TTTACGAAGA 
CTGTCAGGAA ATGAGTACAT CTTATCAGCT AAACAGGTAC ACCCTGTCAG TGGGCATATG 
ATCCTAGCTC TTTCCCTAAG CGATATCCCA CCTCGGATAG CACAAGCAAT GGATAATGAA 
GAAGACTGGG AGTTCGATAT CTTTGAATTA GAGGCTGCTA CACATAAAAG GCCCTTAGTG 
TATCTTGGCC TTAAAATGTT TGCTCGGTTT GGTGTTTGTC AGTTTCTAAA CTGCTCAGAA 
GCTACTTTGA GGTCTTGGCT ACATGTTATC GAGTCAAACT ACCATTCAAG CAACGCCTAT 
CATAATTCAA CGCATTCCGC TGATGTCTTA CATGCCACTG CTTATTTCCT CTGCAAAGAA 
AGAGTGAAGC AAAGCCTGGA TCAGATAGAT GAAGTTGCTG CTCTCATTGC TGCCACAGTC 
CATGATGTGG ATCACCCTGG TCGGACAAAC TCGTTCCTTT GTAATGCAGG GAGTGAGCTG 
GCAATTCTGT ACAACGACAC AGCAGTTTTA GAAAGTCACC ATGCAGCCCT AGCCTTCCAG 
CTGACCACTA GAGATGACAA GTGCAATATA TTCAAAAACA TGGAGAGGAA TGAATATCGC 
ACTTTGCGGC AAGCAATCAT CGACATGGTC TTAGCAACTG AAATGACGAA GCACTTTGAA 
CATGTAAACA AGTTTGTCAA CAGCATCAAT AAACCATTGG CAGCAGTGGA GGAGAATGGG 
GGCAGCATTG AGGAAGATGA GGCTGTTAAA AACATACTGA CATCTCCAGA AAACCGGATC 
CTCATTAAAA GAATGCTTAT TAAGTGCGCA GACATTTCCA ATCCGTGCCG GGCCCTCGAG 
CAGTGCATTG AGTGGGCTGG CCGGATATCA GAAGAGTATT TTGCACAAAC AGATGAGGAG 
AAGAGAAAAG GTTTGCCTGT GGTGATGCCG GTGTTTGACA GAAACACATG TAGCATTCCG 
AAATCCCAGA TCTCCTTTGT TGATTACTTC ATCACTGATA TGTTCGATGC CTGGGATGCA 
TTTGCTGATT TGCCCAACTT GATGCAACAT CTGGATAATA ACTTTAAATA TTGGAAATCA 
CTAGACGAGA GACAGCTAAA GACTCTGCGA CCGCCCCCTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde8a ( XM_012959505.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814959.2 Xenopus tropicalis phosphodiesterase 8A (pde8a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa55137
Clone ID Related Accession (Same CDS sequence)	XM_012959505.3
Accession Version	XM_012959505.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959505.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGTG CTCCAAGCAT CCACATCTCT GACAGCAGGG TGGTGTATCA CAGCGGCAAA 
GAGTCCGACG ATTCCAACTC CCCACACCAG ACCAACACAC TGGCTCAGCA AGGCAACTCC 
GTGCCTGGAT TATTCATTAA ATCCTCTCAC ACATCCACTT ATAAGGTGAG GGCTAATTCC 
TCTAGGAAAG GCAGGAGGGA GAACAGCCCG AGCATGGAGG CAGAGATAAA CAGCAGCACA 
AACAGCAGCA CAAACAGCAG CACAAACAGC AGCACATCCA CCGCTGTAAA GGCAGCTGTA 
AGAGAAGTGC AGTTTGGTCC AATGAAACTC CGCCAAGATC AACTTCAGGT ACTTTTAGTA 
TTTGCCAAAG AAGATAGCCA GAGTAATGGA TTCTGTTGGG CGTGTGAGAA AGCAGGCTTT 
AAGTGTAACA TTGCCAGGAC GCCGGAGACT GCACTGGAAA GCTTTTTAGA TAAACATCAC 
GAAATTATCA TTATTGATCA TAGGCATTCA CGATATTTTG ATGCAGAAGC TCTATGCAGG 
TCCATTAGAG CTACGAAACC CTCAGAAAAC ACTGTCATAG TTGCTGTAGT TAGAAGACAA 
AGTGATCGGG ATGAAGCATC TATTATGCCC CTAATATCTG CTGGATTTAC CAGGAGATAT 
GTAGAAAACT CCAATATTAT GGCCTGTTAC AATGAGCTGA TCCAGTTGGA ATTTGGAGAA 
GTACTTGCAC AATTTAAACT AAGGGCTTGT AATTCGTTAT TTACTACATT AGAAAAAAGT 
CAAGAAGCCA TTGAAATTAC AAGTGAGGAT CATATAATAC AGTATGTGAA TCCTGCTTTT 
GAAAGCACGA TGGGTTATCA GAAGGACGAA CTCATTGGGA AAGAATTAAC TGAAGTGCCT 
ATTAATGAAA AAAAATCTGA TTTCTTAGAT ACCATTAATT GTTGCATTAA AACGGGAAAG 
GAATGGGAAG GTATTTACTA TGCCAAAAAT AAAAATGGTG ACAATATCCA GCAAAATGTG 
AAGATAATTC CTGTTTTTGG ACATCGGGGT AAAATCAGAC ACTATGTGTC TATAAGCCGT 
CCATGCAGTG ACAACAACAA GGCAGAAAAA CAATGTGAGA GAGTTCAGGC CGAATCGCAA 
ACAGATATCC AGACCTGTAG ACACAAGGAC AGGCGGAAAG GTTCCTTAGA CGTCCGATCA 
ACCACCTCCA GAGGAAGCGA TGGTCCAGCT GAATTTTCAC ACGGTGCTCT ACCTGGTAGT 
TCACAGAGAC GGCATTCTTC TATGGCCAGA ATACATTCTA TGACCATTGA AGCCCCTATC 
ACAAAGGTAA TAAATATAAT AAATGCTGCA CAAGAAAACA GCCCTTCGCC TGTGGCAGAA 
GCATTAGATC GAGTTCTAGA GATTCTGAGG ACCACAGAAC TATACTCTCC GCAGCTGGGG 
ACGAAAGATG AAGACCCTCA CACCAACGAC CTGGTCGGAG GTTTGATGAC AGATGGTTTA 
CGAAGACTGT CAGGAAATGA GTACATCTTA TCAGCTAAAC AGGTACACCC TGTCAGTGGG 
CATATGATCC TAGCTCTTTC CCTAAGCGAT ATCCCACCTC GGATAGCACA AGCAATGGAT 
AATGAAGAAG ACTGGGAGTT CGATATCTTT GAATTAGAGG CTGCTACACA TAAAAGGCCC 
TTAGTGTATC TTGGCCTTAA AATGTTTGCT CGGTTTGGTG TTTGTCAGTT TCTAAACTGC 
TCAGAAGCTA CTTTGAGGTC TTGGCTACAT GTTATCGAGT CAAACTACCA TTCAAGCAAC 
GCCTATCATA ATTCAACGCA TTCCGCTGAT GTCTTACATG CCACTGCTTA TTTCCTCTGC 
AAAGAAAGAG TGAAGCAAAG CCTGGATCAG ATAGATGAAG TTGCTGCTCT CATTGCTGCC 
ACAGTCCATG ATGTGGATCA CCCTGGTCGG ACAAACTCGT TCCTTTGTAA TGCAGGGAGT 
GAGCTGGCAA TTCTGTACAA CGACACAGCA GTTTTAGAAA GTCACCATGC AGCCCTAGCC 
TTCCAGCTGA CCACTAGAGA TGACAAGTGC AATATATTCA AAAACATGGA GAGGAATGAA 
TATCGCACTT TGCGGCAAGC AATCATCGAC ATGGTCTTAG CAACTGAAAT GACGAAGCAC 
TTTGAACATG TAAACAAGTT TGTCAACAGC ATCAATAAAC CATTGGCAGC AGTGGAGGAG 
AATGGGGGCA GCATTGAGGA AGATGAGGCT GTTAAAAACA TACTGACATC TCCAGAAAAC 
CGGATCCTCA TTAAAAGAAT GCTTATTAAG TGCGCAGACA TTTCCAATCC GTGCCGGGCC 
CTCGAGCAGT GCATTGAGTG GGCTGGCCGG ATATCAGAAG AGTATTTTGC ACAAACAGAT 
GAGGAGAAGA GAAAAGGTTT GCCTGTGGTG ATGCCGGTGT TTGACAGAAA CACATGTAGC 
ATTCCGAAAT CCCAGATCTC CTTTGTTGAT TACTTCATCA CTGATATGTT CGATGCCTGG 
GATGCATTTG CTGATTTGCC CAACTTGATG CAACATCTGG ATAATAACTT TAAATATTGG 
AAATCACTAG ACGAGAGACA GCTAAAGACT CTGCGACCGC CCCCTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814959.2
CDS	1024..3654
Translation	MGCAPSIHISDSRVVYHSGKESDDSNSPHQTNTLAQQGNSVPGLFIKSSHTSTYKVRANSSRKGRRENSPSMEAEINSSTNSSTNSSTNSSTSTAVKAAVREVQFGPMKLRQDQLQVLLVFAKEDSQSNGFCWACEKAGFKCNIARTPETALESFLDKHHEIIIIDHRHSRYFDAEALCRSIRATKPSENTVIVAVVRRQSDRDEASIMPLISAGFTRRYVENSNIMACYNELIQLEFGEVLAQFKLRACNSLFTTLEKSQEAIEITSEDHIIQYVNPAFESTMGYQKDELIGKELTEVPINEKKSDFLDTINCCIKTGKEWEGIYYAKNKNGDNIQQNVKIIPVFGHRGKIRHYVSISRPCSDNNKAEKQCERVQAESQTDIQTCRHKDRRKGSLDVRSTTSRGSDGPAEFSHGALPGSSQRRHSSMARIHSMTIEAPITKVINIINAAQENSPSPVAEALDRVLEILRTTELYSPQLGTKDEDPHTNDLVGGLMTDGLRRLSGNEYILSAKQVHPVSGHMILALSLSDIPPRIAQAMDNEEDWEFDIFELEAATHKRPLVYLGLKMFARFGVCQFLNCSEATLRSWLHVIESNYHSSNAYHNSTHSADVLHATAYFLCKERVKQSLDQIDEVAALIAATVHDVDHPGRTNSFLCNAGSELAILYNDTAVLESHHAALAFQLTTRDDKCNIFKNMERNEYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPLAAVEENGGSIEEDEAVKNILTSPENRILIKRMLIKCADISNPCRALEQCIEWAGRISEEYFAQTDEEKRKGLPVVMPVFDRNTCSIPKSQISFVDYFITDMFDAWDAFADLPNLMQHLDNNFKYWKSLDERQLKTLRPPPE

Target ORF information:

RefSeq Version	XM_012959505.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 8A (pde8a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959505.3

ORF Insert Sequence:

ATGGGCTGTG CTCCAAGCAT CCACATCTCT GACAGCAGGG TGGTGTATCA CAGCGGCAAA 
GAGTCCGACG ATTCCAACTC CCCACACCAG ACCAACACAC TGGCTCAGCA AGGCAACTCC 
GTGCCTGGAT TATTCATTAA ATCCTCTCAC ACATCCACTT ATAAGGTGAG GGCTAATTCC 
TCTAGGAAAG GCAGGAGGGA GAACAGCCCG AGCATGGAGG CAGAGATAAA CAGCAGCACA 
AACAGCAGCA CAAACAGCAG CACAAACAGC AGCACATCCA CCGCTGTAAA GGCAGCTGTA 
AGAGAAGTGC AGTTTGGTCC AATGAAACTC CGCCAAGATC AACTTCAGGT ACTTTTAGTA 
TTTGCCAAAG AAGATAGCCA GAGTAATGGA TTCTGTTGGG CGTGTGAGAA AGCAGGCTTT 
AAGTGTAACA TTGCCAGGAC GCCGGAGACT GCACTGGAAA GCTTTTTAGA TAAACATCAC 
GAAATTATCA TTATTGATCA TAGGCATTCA CGATATTTTG ATGCAGAAGC TCTATGCAGG 
TCCATTAGAG CTACGAAACC CTCAGAAAAC ACTGTCATAG TTGCTGTAGT TAGAAGACAA 
AGTGATCGGG ATGAAGCATC TATTATGCCC CTAATATCTG CTGGATTTAC CAGGAGATAT 
GTAGAAAACT CCAATATTAT GGCCTGTTAC AATGAGCTGA TCCAGTTGGA ATTTGGAGAA 
GTACTTGCAC AATTTAAACT AAGGGCTTGT AATTCGTTAT TTACTACATT AGAAAAAAGT 
CAAGAAGCCA TTGAAATTAC AAGTGAGGAT CATATAATAC AGTATGTGAA TCCTGCTTTT 
GAAAGCACGA TGGGTTATCA GAAGGACGAA CTCATTGGGA AAGAATTAAC TGAAGTGCCT 
ATTAATGAAA AAAAATCTGA TTTCTTAGAT ACCATTAATT GTTGCATTAA AACGGGAAAG 
GAATGGGAAG GTATTTACTA TGCCAAAAAT AAAAATGGTG ACAATATCCA GCAAAATGTG 
AAGATAATTC CTGTTTTTGG ACATCGGGGT AAAATCAGAC ACTATGTGTC TATAAGCCGT 
CCATGCAGTG ACAACAACAA GGCAGAAAAA CAATGTGAGA GAGTTCAGGC CGAATCGCAA 
ACAGATATCC AGACCTGTAG ACACAAGGAC AGGCGGAAAG GTTCCTTAGA CGTCCGATCA 
ACCACCTCCA GAGGAAGCGA TGGTCCAGCT GAATTTTCAC ACGGTGCTCT ACCTGGTAGT 
TCACAGAGAC GGCATTCTTC TATGGCCAGA ATACATTCTA TGACCATTGA AGCCCCTATC 
ACAAAGGTAA TAAATATAAT AAATGCTGCA CAAGAAAACA GCCCTTCGCC TGTGGCAGAA 
GCATTAGATC GAGTTCTAGA GATTCTGAGG ACCACAGAAC TATACTCTCC GCAGCTGGGG 
ACGAAAGATG AAGACCCTCA CACCAACGAC CTGGTCGGAG GTTTGATGAC AGATGGTTTA 
CGAAGACTGT CAGGAAATGA GTACATCTTA TCAGCTAAAC AGGTACACCC TGTCAGTGGG 
CATATGATCC TAGCTCTTTC CCTAAGCGAT ATCCCACCTC GGATAGCACA AGCAATGGAT 
AATGAAGAAG ACTGGGAGTT CGATATCTTT GAATTAGAGG CTGCTACACA TAAAAGGCCC 
TTAGTGTATC TTGGCCTTAA AATGTTTGCT CGGTTTGGTG TTTGTCAGTT TCTAAACTGC 
TCAGAAGCTA CTTTGAGGTC TTGGCTACAT GTTATCGAGT CAAACTACCA TTCAAGCAAC 
GCCTATCATA ATTCAACGCA TTCCGCTGAT GTCTTACATG CCACTGCTTA TTTCCTCTGC 
AAAGAAAGAG TGAAGCAAAG CCTGGATCAG ATAGATGAAG TTGCTGCTCT CATTGCTGCC 
ACAGTCCATG ATGTGGATCA CCCTGGTCGG ACAAACTCGT TCCTTTGTAA TGCAGGGAGT 
GAGCTGGCAA TTCTGTACAA CGACACAGCA GTTTTAGAAA GTCACCATGC AGCCCTAGCC 
TTCCAGCTGA CCACTAGAGA TGACAAGTGC AATATATTCA AAAACATGGA GAGGAATGAA 
TATCGCACTT TGCGGCAAGC AATCATCGAC ATGGTCTTAG CAACTGAAAT GACGAAGCAC 
TTTGAACATG TAAACAAGTT TGTCAACAGC ATCAATAAAC CATTGGCAGC AGTGGAGGAG 
AATGGGGGCA GCATTGAGGA AGATGAGGCT GTTAAAAACA TACTGACATC TCCAGAAAAC 
CGGATCCTCA TTAAAAGAAT GCTTATTAAG TGCGCAGACA TTTCCAATCC GTGCCGGGCC 
CTCGAGCAGT GCATTGAGTG GGCTGGCCGG ATATCAGAAG AGTATTTTGC ACAAACAGAT 
GAGGAGAAGA GAAAAGGTTT GCCTGTGGTG ATGCCGGTGT TTGACAGAAA CACATGTAGC 
ATTCCGAAAT CCCAGATCTC CTTTGTTGAT TACTTCATCA CTGATATGTT CGATGCCTGG 
GATGCATTTG CTGATTTGCC CAACTTGATG CAACATCTGG ATAATAACTT TAAATATTGG 
AAATCACTAG ACGAGAGACA GCTAAAGACT CTGCGACCGC CCCCTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde8a ( XM_002939622.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939668.3 Xenopus tropicalis phosphodiesterase 8A (pde8a), transcript variant X2, mRNA.

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CloneID	OXa55138
Clone ID Related Accession (Same CDS sequence)	XM_002939622.5
Accession Version	XM_002939622.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002939622.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGTG CTCCAAGCAT CCACATCTCT GACAGCAGGG TGGTGTATCA CAGCGGCAAA 
GAGTCCGACG ATTCCAACTC CCCACACCAG ACCAACACAC TGGCTCAGCA AGGCAACTCC 
GTGCCTGGAT TATTCATTAA ATCCTCTCAC ACATCCACTT ATAAGGTGAG GGCTAATTCC 
TCTAGGAAAG GCAGGAGGGA GAACAGCCCG AGCATGGAGG CAGAGATAAA CAGCAGCACA 
AACAGCAGCA CAAACAGCAG CACAAACAGC AGCACATCCA CCGCTGTAAA GGCAGCTGTA 
AGAGAAGTGC AGTTTGGTCC AATGAAACTC CGCCAAGATC AACTTCAGGT ACTTTTAGTA 
TTTGCCAAAG AAGATAGCCA GAGTAATGGA TTCTGTTGGG CGTGTGAGAA AGCAGGCTTT 
AAGTGTAACA TTGCCAGGAC GCCGGAGACT GCACTGGAAA GCTTTTTAGA TAAACATCAC 
GAAATTATCA TTATTGATCA TAGGCATTCA CGATATTTTG ATGCAGAAGC TCTATGCAGG 
TCCATTAGAG CTACGAAACC CTCAGAAAAC ACTGTCATAG TTGCTGTAGT TAGAAGACAA 
AGTGATCGGG ATGAAGCATC TATTATGCCC CTAATATCTG CTGGATTTAC CAGGAGATAT 
GTAGAAAACT CCAATATTAT GGCCTGTTAC AATGAGCTGA TCCAGTTGGA ATTTGGAGAA 
GTACTTGCAC AATTTAAACT AAGGGCTTGT AATTCGTTAT TTACTACATT AGAAAAAAGT 
CAAGAAGCCA TTGAAATTAC AAGTGAGGAT CATATAATAC AGTATGTGAA TCCTGCTTTT 
GAAAGCACGA TGGGTTATCA GAAGGACGAA CTCATTGGGA AAGAATTAAC TGAAGTGCCT 
ATTAATGAAA AAAAATCTGA TTTCTTAGAT ACCATTAATT GTTGCATTAA AACGGGAAAG 
GAATGGGAAG GTATTTACTA TGCCAAAAAT AAAAATGGTG ACAATATCCA GCAAAATGTG 
AAGATAATTC CTGTTTTTGG ACATCGGGGT AAAATCAGAC ACTATGTGTC TATAAGCCGT 
CCATGCAGTG ACAACAACAA GGCAGAAAAA CAATGTGAGA GAGTTCAGGC CGAATCGCAA 
ACAGATATCC AGACCTGTAG ACACAAGGAC AGGCGGAAAG GTTCCTTAGA CGTCCGATCA 
ACCACCTCCA GAGGAAGCGA TGGTAGTTCA CAGAGACGGC ATTCTTCTAT GGCCAGAATA 
CATTCTATGA CCATTGAAGC CCCTATCACA AAGGTAATAA ATATAATAAA TGCTGCACAA 
GAAAACAGCC CTTCGCCTGT GGCAGAAGCA TTAGATCGAG TTCTAGAGAT TCTGAGGACC 
ACAGAACTAT ACTCTCCGCA GCTGGGGACG AAAGATGAAG ACCCTCACAC CAACGACCTG 
GTCGGAGGTT TGATGACAGA TGGTTTACGA AGACTGTCAG GAAATGAGTA CATCTTATCA 
GCTAAACAGG TACACCCTGT CAGTGGGCAT ATGATCCTAG CTCTTTCCCT AAGCGATATC 
CCACCTCGGA TAGCACAAGC AATGGATAAT GAAGAAGACT GGGAGTTCGA TATCTTTGAA 
TTAGAGGCTG CTACACATAA AAGGCCCTTA GTGTATCTTG GCCTTAAAAT GTTTGCTCGG 
TTTGGTGTTT GTCAGTTTCT AAACTGCTCA GAAGCTACTT TGAGGTCTTG GCTACATGTT 
ATCGAGTCAA ACTACCATTC AAGCAACGCC TATCATAATT CAACGCATTC CGCTGATGTC 
TTACATGCCA CTGCTTATTT CCTCTGCAAA GAAAGAGTGA AGCAAAGCCT GGATCAGATA 
GATGAAGTTG CTGCTCTCAT TGCTGCCACA GTCCATGATG TGGATCACCC TGGTCGGACA 
AACTCGTTCC TTTGTAATGC AGGGAGTGAG CTGGCAATTC TGTACAACGA CACAGCAGTT 
TTAGAAAGTC ACCATGCAGC CCTAGCCTTC CAGCTGACCA CTAGAGATGA CAAGTGCAAT 
ATATTCAAAA ACATGGAGAG GAATGAATAT CGCACTTTGC GGCAAGCAAT CATCGACATG 
GTCTTAGCAA CTGAAATGAC GAAGCACTTT GAACATGTAA ACAAGTTTGT CAACAGCATC 
AATAAACCAT TGGCAGCAGT GGAGGAGAAT GGGGGCAGCA TTGAGGAAGA TGAGGCTGTT 
AAAAACATAC TGACATCTCC AGAAAACCGG ATCCTCATTA AAAGAATGCT TATTAAGTGC 
GCAGACATTT CCAATCCGTG CCGGGCCCTC GAGCAGTGCA TTGAGTGGGC TGGCCGGATA 
TCAGAAGAGT ATTTTGCACA AACAGATGAG GAGAAGAGAA AAGGTTTGCC TGTGGTGATG 
CCGGTGTTTG ACAGAAACAC ATGTAGCATT CCGAAATCCC AGATCTCCTT TGTTGATTAC 
TTCATCACTG ATATGTTCGA TGCCTGGGAT GCATTTGCTG ATTTGCCCAA CTTGATGCAA 
CATCTGGATA ATAACTTTAA ATATTGGAAA TCACTAGACG AGAGACAGCT AAAGACTCTG 
CGACCGCCCC CTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939668.3
CDS	1022..3619
Translation	MGCAPSIHISDSRVVYHSGKESDDSNSPHQTNTLAQQGNSVPGLFIKSSHTSTYKVRANSSRKGRRENSPSMEAEINSSTNSSTNSSTNSSTSTAVKAAVREVQFGPMKLRQDQLQVLLVFAKEDSQSNGFCWACEKAGFKCNIARTPETALESFLDKHHEIIIIDHRHSRYFDAEALCRSIRATKPSENTVIVAVVRRQSDRDEASIMPLISAGFTRRYVENSNIMACYNELIQLEFGEVLAQFKLRACNSLFTTLEKSQEAIEITSEDHIIQYVNPAFESTMGYQKDELIGKELTEVPINEKKSDFLDTINCCIKTGKEWEGIYYAKNKNGDNIQQNVKIIPVFGHRGKIRHYVSISRPCSDNNKAEKQCERVQAESQTDIQTCRHKDRRKGSLDVRSTTSRGSDGSSQRRHSSMARIHSMTIEAPITKVINIINAAQENSPSPVAEALDRVLEILRTTELYSPQLGTKDEDPHTNDLVGGLMTDGLRRLSGNEYILSAKQVHPVSGHMILALSLSDIPPRIAQAMDNEEDWEFDIFELEAATHKRPLVYLGLKMFARFGVCQFLNCSEATLRSWLHVIESNYHSSNAYHNSTHSADVLHATAYFLCKERVKQSLDQIDEVAALIAATVHDVDHPGRTNSFLCNAGSELAILYNDTAVLESHHAALAFQLTTRDDKCNIFKNMERNEYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPLAAVEENGGSIEEDEAVKNILTSPENRILIKRMLIKCADISNPCRALEQCIEWAGRISEEYFAQTDEEKRKGLPVVMPVFDRNTCSIPKSQISFVDYFITDMFDAWDAFADLPNLMQHLDNNFKYWKSLDERQLKTLRPPPE

Target ORF information:

RefSeq Version	XM_002939622.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 8A (pde8a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939622.5

ORF Insert Sequence:

ATGGGCTGTG CTCCAAGCAT CCACATCTCT GACAGCAGGG TGGTGTATCA CAGCGGCAAA 
GAGTCCGACG ATTCCAACTC CCCACACCAG ACCAACACAC TGGCTCAGCA AGGCAACTCC 
GTGCCTGGAT TATTCATTAA ATCCTCTCAC ACATCCACTT ATAAGGTGAG GGCTAATTCC 
TCTAGGAAAG GCAGGAGGGA GAACAGCCCG AGCATGGAGG CAGAGATAAA CAGCAGCACA 
AACAGCAGCA CAAACAGCAG CACAAACAGC AGCACATCCA CCGCTGTAAA GGCAGCTGTA 
AGAGAAGTGC AGTTTGGTCC AATGAAACTC CGCCAAGATC AACTTCAGGT ACTTTTAGTA 
TTTGCCAAAG AAGATAGCCA GAGTAATGGA TTCTGTTGGG CGTGTGAGAA AGCAGGCTTT 
AAGTGTAACA TTGCCAGGAC GCCGGAGACT GCACTGGAAA GCTTTTTAGA TAAACATCAC 
GAAATTATCA TTATTGATCA TAGGCATTCA CGATATTTTG ATGCAGAAGC TCTATGCAGG 
TCCATTAGAG CTACGAAACC CTCAGAAAAC ACTGTCATAG TTGCTGTAGT TAGAAGACAA 
AGTGATCGGG ATGAAGCATC TATTATGCCC CTAATATCTG CTGGATTTAC CAGGAGATAT 
GTAGAAAACT CCAATATTAT GGCCTGTTAC AATGAGCTGA TCCAGTTGGA ATTTGGAGAA 
GTACTTGCAC AATTTAAACT AAGGGCTTGT AATTCGTTAT TTACTACATT AGAAAAAAGT 
CAAGAAGCCA TTGAAATTAC AAGTGAGGAT CATATAATAC AGTATGTGAA TCCTGCTTTT 
GAAAGCACGA TGGGTTATCA GAAGGACGAA CTCATTGGGA AAGAATTAAC TGAAGTGCCT 
ATTAATGAAA AAAAATCTGA TTTCTTAGAT ACCATTAATT GTTGCATTAA AACGGGAAAG 
GAATGGGAAG GTATTTACTA TGCCAAAAAT AAAAATGGTG ACAATATCCA GCAAAATGTG 
AAGATAATTC CTGTTTTTGG ACATCGGGGT AAAATCAGAC ACTATGTGTC TATAAGCCGT 
CCATGCAGTG ACAACAACAA GGCAGAAAAA CAATGTGAGA GAGTTCAGGC CGAATCGCAA 
ACAGATATCC AGACCTGTAG ACACAAGGAC AGGCGGAAAG GTTCCTTAGA CGTCCGATCA 
ACCACCTCCA GAGGAAGCGA TGGTAGTTCA CAGAGACGGC ATTCTTCTAT GGCCAGAATA 
CATTCTATGA CCATTGAAGC CCCTATCACA AAGGTAATAA ATATAATAAA TGCTGCACAA 
GAAAACAGCC CTTCGCCTGT GGCAGAAGCA TTAGATCGAG TTCTAGAGAT TCTGAGGACC 
ACAGAACTAT ACTCTCCGCA GCTGGGGACG AAAGATGAAG ACCCTCACAC CAACGACCTG 
GTCGGAGGTT TGATGACAGA TGGTTTACGA AGACTGTCAG GAAATGAGTA CATCTTATCA 
GCTAAACAGG TACACCCTGT CAGTGGGCAT ATGATCCTAG CTCTTTCCCT AAGCGATATC 
CCACCTCGGA TAGCACAAGC AATGGATAAT GAAGAAGACT GGGAGTTCGA TATCTTTGAA 
TTAGAGGCTG CTACACATAA AAGGCCCTTA GTGTATCTTG GCCTTAAAAT GTTTGCTCGG 
TTTGGTGTTT GTCAGTTTCT AAACTGCTCA GAAGCTACTT TGAGGTCTTG GCTACATGTT 
ATCGAGTCAA ACTACCATTC AAGCAACGCC TATCATAATT CAACGCATTC CGCTGATGTC 
TTACATGCCA CTGCTTATTT CCTCTGCAAA GAAAGAGTGA AGCAAAGCCT GGATCAGATA 
GATGAAGTTG CTGCTCTCAT TGCTGCCACA GTCCATGATG TGGATCACCC TGGTCGGACA 
AACTCGTTCC TTTGTAATGC AGGGAGTGAG CTGGCAATTC TGTACAACGA CACAGCAGTT 
TTAGAAAGTC ACCATGCAGC CCTAGCCTTC CAGCTGACCA CTAGAGATGA CAAGTGCAAT 
ATATTCAAAA ACATGGAGAG GAATGAATAT CGCACTTTGC GGCAAGCAAT CATCGACATG 
GTCTTAGCAA CTGAAATGAC GAAGCACTTT GAACATGTAA ACAAGTTTGT CAACAGCATC 
AATAAACCAT TGGCAGCAGT GGAGGAGAAT GGGGGCAGCA TTGAGGAAGA TGAGGCTGTT 
AAAAACATAC TGACATCTCC AGAAAACCGG ATCCTCATTA AAAGAATGCT TATTAAGTGC 
GCAGACATTT CCAATCCGTG CCGGGCCCTC GAGCAGTGCA TTGAGTGGGC TGGCCGGATA 
TCAGAAGAGT ATTTTGCACA AACAGATGAG GAGAAGAGAA AAGGTTTGCC TGTGGTGATG 
CCGGTGTTTG ACAGAAACAC ATGTAGCATT CCGAAATCCC AGATCTCCTT TGTTGATTAC 
TTCATCACTG ATATGTTCGA TGCCTGGGAT GCATTTGCTG ATTTGCCCAA CTTGATGCAA 
CATCTGGATA ATAACTTTAA ATATTGGAAA TCACTAGACG AGAGACAGCT AAAGACTCTG 
CGACCGCCCC CTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde8a ( XM_012959506.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814960.2 Xenopus tropicalis phosphodiesterase 8A (pde8a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa55139
Clone ID Related Accession (Same CDS sequence)	XM_012959506.3
Accession Version	XM_012959506.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959506.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGTG CTCCAAGCAT CCACATCTCT GACAGCAGGG TGGTGTATCA CAGCGGCAAA 
GAGTCCGACG ATTCCAACTC CCCACACCAG ACCAACACAC TGGCTCAGCA AGGCAACTCC 
GTGCCTGGAT TATTCATTAA ATCCTCTCAC ACATCCACTT ATAAGGCAGC TGTAAGAGAA 
GTGCAGTTTG GTCCAATGAA ACTCCGCCAA GATCAACTTC AGGTACTTTT AGTATTTGCC 
AAAGAAGATA GCCAGAGTAA TGGATTCTGT TGGGCGTGTG AGAAAGCAGG CTTTAAGTGT 
AACATTGCCA GGACGCCGGA GACTGCACTG GAAAGCTTTT TAGATAAACA TCACGAAATT 
ATCATTATTG ATCATAGGCA TTCACGATAT TTTGATGCAG AAGCTCTATG CAGGTCCATT 
AGAGCTACGA AACCCTCAGA AAACACTGTC ATAGTTGCTG TAGTTAGAAG ACAAAGTGAT 
CGGGATGAAG CATCTATTAT GCCCCTAATA TCTGCTGGAT TTACCAGGAG ATATGTAGAA 
AACTCCAATA TTATGGCCTG TTACAATGAG CTGATCCAGT TGGAATTTGG AGAAGTACTT 
GCACAATTTA AACTAAGGGC TTGTAATTCG TTATTTACTA CATTAGAAAA AAGTCAAGAA 
GCCATTGAAA TTACAAGTGA GGATCATATA ATACAGTATG TGAATCCTGC TTTTGAAAGC 
ACGATGGGTT ATCAGAAGGA CGAACTCATT GGGAAAGAAT TAACTGAAGT GCCTATTAAT 
GAAAAAAAAT CTGATTTCTT AGATACCATT AATTGTTGCA TTAAAACGGG AAAGGAATGG 
GAAGGTATTT ACTATGCCAA AAATAAAAAT GGTGACAATA TCCAGCAAAA TGTGAAGATA 
ATTCCTGTTT TTGGACATCG GGGTAAAATC AGACACTATG TGTCTATAAG CCGTCCATGC 
AGTGACAACA ACAAGGCAGA AAAACAATGT GAGAGAGTTC AGGCCGAATC GCAAACAGAT 
ATCCAGACCT GTAGACACAA GGACAGGCGG AAAGGTTCCT TAGACGTCCG ATCAACCACC 
TCCAGAGGAA GCGATGGTCC AGCTGAATTT TCACACGGTG CTCTACCTGG TAGTTCACAG 
AGACGGCATT CTTCTATGGC CAGAATACAT TCTATGACCA TTGAAGCCCC TATCACAAAG 
GTAATAAATA TAATAAATGC TGCACAAGAA AACAGCCCTT CGCCTGTGGC AGAAGCATTA 
GATCGAGTTC TAGAGATTCT GAGGACCACA GAACTATACT CTCCGCAGCT GGGGACGAAA 
GATGAAGACC CTCACACCAA CGACCTGGTC GGAGGTTTGA TGACAGATGG TTTACGAAGA 
CTGTCAGGAA ATGAGTACAT CTTATCAGCT AAACAGGTAC ACCCTGTCAG TGGGCATATG 
ATCCTAGCTC TTTCCCTAAG CGATATCCCA CCTCGGATAG CACAAGCAAT GGATAATGAA 
GAAGACTGGG AGTTCGATAT CTTTGAATTA GAGGCTGCTA CACATAAAAG GCCCTTAGTG 
TATCTTGGCC TTAAAATGTT TGCTCGGTTT GGTGTTTGTC AGTTTCTAAA CTGCTCAGAA 
GCTACTTTGA GGTCTTGGCT ACATGTTATC GAGTCAAACT ACCATTCAAG CAACGCCTAT 
CATAATTCAA CGCATTCCGC TGATGTCTTA CATGCCACTG CTTATTTCCT CTGCAAAGAA 
AGAGTGAAGC AAAGCCTGGA TCAGATAGAT GAAGTTGCTG CTCTCATTGC TGCCACAGTC 
CATGATGTGG ATCACCCTGG TCGGACAAAC TCGTTCCTTT GTAATGCAGG GAGTGAGCTG 
GCAATTCTGT ACAACGACAC AGCAGTTTTA GAAAGTCACC ATGCAGCCCT AGCCTTCCAG 
CTGACCACTA GAGATGACAA GTGCAATATA TTCAAAAACA TGGAGAGGAA TGAATATCGC 
ACTTTGCGGC AAGCAATCAT CGACATGGTC TTAGCAACTG AAATGACGAA GCACTTTGAA 
CATGTAAACA AGTTTGTCAA CAGCATCAAT AAACCATTGG CAGCAGTGGA GGAGAATGGG 
GGCAGCATTG AGGAAGATGA GGCTGTTAAA AACATACTGA CATCTCCAGA AAACCGGATC 
CTCATTAAAA GAATGCTTAT TAAGTGCGCA GACATTTCCA ATCCGTGCCG GGCCCTCGAG 
CAGTGCATTG AGTGGGCTGG CCGGATATCA GAAGAGTATT TTGCACAAAC AGATGAGGAG 
AAGAGAAAAG GTTTGCCTGT GGTGATGCCG GTGTTTGACA GAAACACATG TAGCATTCCG 
AAATCCCAGA TCTCCTTTGT TGATTACTTC ATCACTGATA TGTTCGATGC CTGGGATGCA 
TTTGCTGATT TGCCCAACTT GATGCAACAT CTGGATAATA ACTTTAAATA TTGGAAATCA 
CTAGACGAGA GACAGCTAAA GACTCTGCGA CCGCCCCCTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814960.2
CDS	1024..3528
Translation	MGCAPSIHISDSRVVYHSGKESDDSNSPHQTNTLAQQGNSVPGLFIKSSHTSTYKAAVREVQFGPMKLRQDQLQVLLVFAKEDSQSNGFCWACEKAGFKCNIARTPETALESFLDKHHEIIIIDHRHSRYFDAEALCRSIRATKPSENTVIVAVVRRQSDRDEASIMPLISAGFTRRYVENSNIMACYNELIQLEFGEVLAQFKLRACNSLFTTLEKSQEAIEITSEDHIIQYVNPAFESTMGYQKDELIGKELTEVPINEKKSDFLDTINCCIKTGKEWEGIYYAKNKNGDNIQQNVKIIPVFGHRGKIRHYVSISRPCSDNNKAEKQCERVQAESQTDIQTCRHKDRRKGSLDVRSTTSRGSDGPAEFSHGALPGSSQRRHSSMARIHSMTIEAPITKVINIINAAQENSPSPVAEALDRVLEILRTTELYSPQLGTKDEDPHTNDLVGGLMTDGLRRLSGNEYILSAKQVHPVSGHMILALSLSDIPPRIAQAMDNEEDWEFDIFELEAATHKRPLVYLGLKMFARFGVCQFLNCSEATLRSWLHVIESNYHSSNAYHNSTHSADVLHATAYFLCKERVKQSLDQIDEVAALIAATVHDVDHPGRTNSFLCNAGSELAILYNDTAVLESHHAALAFQLTTRDDKCNIFKNMERNEYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPLAAVEENGGSIEEDEAVKNILTSPENRILIKRMLIKCADISNPCRALEQCIEWAGRISEEYFAQTDEEKRKGLPVVMPVFDRNTCSIPKSQISFVDYFITDMFDAWDAFADLPNLMQHLDNNFKYWKSLDERQLKTLRPPPE

Target ORF information:

RefSeq Version	XM_012959506.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 8A (pde8a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959506.3

ORF Insert Sequence:

ATGGGCTGTG CTCCAAGCAT CCACATCTCT GACAGCAGGG TGGTGTATCA CAGCGGCAAA 
GAGTCCGACG ATTCCAACTC CCCACACCAG ACCAACACAC TGGCTCAGCA AGGCAACTCC 
GTGCCTGGAT TATTCATTAA ATCCTCTCAC ACATCCACTT ATAAGGCAGC TGTAAGAGAA 
GTGCAGTTTG GTCCAATGAA ACTCCGCCAA GATCAACTTC AGGTACTTTT AGTATTTGCC 
AAAGAAGATA GCCAGAGTAA TGGATTCTGT TGGGCGTGTG AGAAAGCAGG CTTTAAGTGT 
AACATTGCCA GGACGCCGGA GACTGCACTG GAAAGCTTTT TAGATAAACA TCACGAAATT 
ATCATTATTG ATCATAGGCA TTCACGATAT TTTGATGCAG AAGCTCTATG CAGGTCCATT 
AGAGCTACGA AACCCTCAGA AAACACTGTC ATAGTTGCTG TAGTTAGAAG ACAAAGTGAT 
CGGGATGAAG CATCTATTAT GCCCCTAATA TCTGCTGGAT TTACCAGGAG ATATGTAGAA 
AACTCCAATA TTATGGCCTG TTACAATGAG CTGATCCAGT TGGAATTTGG AGAAGTACTT 
GCACAATTTA AACTAAGGGC TTGTAATTCG TTATTTACTA CATTAGAAAA AAGTCAAGAA 
GCCATTGAAA TTACAAGTGA GGATCATATA ATACAGTATG TGAATCCTGC TTTTGAAAGC 
ACGATGGGTT ATCAGAAGGA CGAACTCATT GGGAAAGAAT TAACTGAAGT GCCTATTAAT 
GAAAAAAAAT CTGATTTCTT AGATACCATT AATTGTTGCA TTAAAACGGG AAAGGAATGG 
GAAGGTATTT ACTATGCCAA AAATAAAAAT GGTGACAATA TCCAGCAAAA TGTGAAGATA 
ATTCCTGTTT TTGGACATCG GGGTAAAATC AGACACTATG TGTCTATAAG CCGTCCATGC 
AGTGACAACA ACAAGGCAGA AAAACAATGT GAGAGAGTTC AGGCCGAATC GCAAACAGAT 
ATCCAGACCT GTAGACACAA GGACAGGCGG AAAGGTTCCT TAGACGTCCG ATCAACCACC 
TCCAGAGGAA GCGATGGTCC AGCTGAATTT TCACACGGTG CTCTACCTGG TAGTTCACAG 
AGACGGCATT CTTCTATGGC CAGAATACAT TCTATGACCA TTGAAGCCCC TATCACAAAG 
GTAATAAATA TAATAAATGC TGCACAAGAA AACAGCCCTT CGCCTGTGGC AGAAGCATTA 
GATCGAGTTC TAGAGATTCT GAGGACCACA GAACTATACT CTCCGCAGCT GGGGACGAAA 
GATGAAGACC CTCACACCAA CGACCTGGTC GGAGGTTTGA TGACAGATGG TTTACGAAGA 
CTGTCAGGAA ATGAGTACAT CTTATCAGCT AAACAGGTAC ACCCTGTCAG TGGGCATATG 
ATCCTAGCTC TTTCCCTAAG CGATATCCCA CCTCGGATAG CACAAGCAAT GGATAATGAA 
GAAGACTGGG AGTTCGATAT CTTTGAATTA GAGGCTGCTA CACATAAAAG GCCCTTAGTG 
TATCTTGGCC TTAAAATGTT TGCTCGGTTT GGTGTTTGTC AGTTTCTAAA CTGCTCAGAA 
GCTACTTTGA GGTCTTGGCT ACATGTTATC GAGTCAAACT ACCATTCAAG CAACGCCTAT 
CATAATTCAA CGCATTCCGC TGATGTCTTA CATGCCACTG CTTATTTCCT CTGCAAAGAA 
AGAGTGAAGC AAAGCCTGGA TCAGATAGAT GAAGTTGCTG CTCTCATTGC TGCCACAGTC 
CATGATGTGG ATCACCCTGG TCGGACAAAC TCGTTCCTTT GTAATGCAGG GAGTGAGCTG 
GCAATTCTGT ACAACGACAC AGCAGTTTTA GAAAGTCACC ATGCAGCCCT AGCCTTCCAG 
CTGACCACTA GAGATGACAA GTGCAATATA TTCAAAAACA TGGAGAGGAA TGAATATCGC 
ACTTTGCGGC AAGCAATCAT CGACATGGTC TTAGCAACTG AAATGACGAA GCACTTTGAA 
CATGTAAACA AGTTTGTCAA CAGCATCAAT AAACCATTGG CAGCAGTGGA GGAGAATGGG 
GGCAGCATTG AGGAAGATGA GGCTGTTAAA AACATACTGA CATCTCCAGA AAACCGGATC 
CTCATTAAAA GAATGCTTAT TAAGTGCGCA GACATTTCCA ATCCGTGCCG GGCCCTCGAG 
CAGTGCATTG AGTGGGCTGG CCGGATATCA GAAGAGTATT TTGCACAAAC AGATGAGGAG 
AAGAGAAAAG GTTTGCCTGT GGTGATGCCG GTGTTTGACA GAAACACATG TAGCATTCCG 
AAATCCCAGA TCTCCTTTGT TGATTACTTC ATCACTGATA TGTTCGATGC CTGGGATGCA 
TTTGCTGATT TGCCCAACTT GATGCAACAT CTGGATAATA ACTTTAAATA TTGGAAATCA 
CTAGACGAGA GACAGCTAAA GACTCTGCGA CCGCCCCCTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde8a ( XM_031899209.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755069.1 Xenopus tropicalis phosphodiesterase 8A (pde8a), transcript variant X4, mRNA.

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CloneID	OXa55140
Clone ID Related Accession (Same CDS sequence)	XM_031899209.1
Accession Version	XM_031899209.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTGGA AGAAGGTGAA AACTTTAGGA GGAAGCAGCA GAGGACACAA TGGTCCTTTA 
AATCAGGCAG CTGTAAGAGA AGTGCAGTTT GGTCCAATGA AACTCCGCCA AGATCAACTT 
CAGGTACTTT TAGTATTTGC CAAAGAAGAT AGCCAGAGTA ATGGATTCTG TTGGGCGTGT 
GAGAAAGCAG GCTTTAAGTG TAACATTGCC AGGACGCCGG AGACTGCACT GGAAAGCTTT 
TTAGATAAAC ATCACGAAAT TATCATTATT GATCATAGGC ATTCACGATA TTTTGATGCA 
GAAGCTCTAT GCAGGTCCAT TAGAGCTACG AAACCCTCAG AAAACACTGT CATAGTTGCT 
GTAGTTAGAA GACAAAGTGA TCGGGATGAA GCATCTATTA TGCCCCTAAT ATCTGCTGGA 
TTTACCAGGA GATATGTAGA AAACTCCAAT ATTATGGCCT GTTACAATGA GCTGATCCAG 
TTGGAATTTG GAGAAGTACT TGCACAATTT AAACTAAGGG CTTGTAATTC GTTATTTACT 
ACATTAGAAA AAAGTCAAGA AGCCATTGAA ATTACAAGTG AGGATCATAT AATACAGTAT 
GTGAATCCTG CTTTTGAAAG CACGATGGGT TATCAGAAGG ACGAACTCAT TGGGAAAGAA 
TTAACTGAAG TGCCTATTAA TGAAAAAAAA TCTGATTTCT TAGATACCAT TAATTGTTGC 
ATTAAAACGG GAAAGGAATG GGAAGGTATT TACTATGCCA AAAATAAAAA TGGTGACAAT 
ATCCAGCAAA ATGTGAAGAT AATTCCTGTT TTTGGACATC GGGGTAAAAT CAGACACTAT 
GTGTCTATAA GCCGTCCATG CAGTGACAAC AACAAGGCAG AAAAACAATG TGAGAGAGTT 
CAGGCCGAAT CGCAAACAGA TATCCAGACC TGTAGACACA AGGACAGGCG GAAAGGTTCC 
TTAGACGTCC GATCAACCAC CTCCAGAGGA AGCGATGGTC CAGCTGAATT TTCACACGGT 
GCTCTACCTG GTAGTTCACA GAGACGGCAT TCTTCTATGG CCAGAATACA TTCTATGACC 
ATTGAAGCCC CTATCACAAA GGTAATAAAT ATAATAAATG CTGCACAAGA AAACAGCCCT 
TCGCCTGTGG CAGAAGCATT AGATCGAGTT CTAGAGATTC TGAGGACCAC AGAACTATAC 
TCTCCGCAGC TGGGGACGAA AGATGAAGAC CCTCACACCA ACGACCTGGT CGGAGGTTTG 
ATGACAGATG GTTTACGAAG ACTGTCAGGA AATGAGTACA TCTTATCAGC TAAACAGGTA 
CACCCTGTCA GTGGGCATAT GATCCTAGCT CTTTCCCTAA GCGATATCCC ACCTCGGATA 
GCACAAGCAA TGGATAATGA AGAAGACTGG GAGTTCGATA TCTTTGAATT AGAGGCTGCT 
ACACATAAAA GGCCCTTAGT GTATCTTGGC CTTAAAATGT TTGCTCGGTT TGGTGTTTGT 
CAGTTTCTAA ACTGCTCAGA AGCTACTTTG AGGTCTTGGC TACATGTTAT CGAGTCAAAC 
TACCATTCAA GCAACGCCTA TCATAATTCA ACGCATTCCG CTGATGTCTT ACATGCCACT 
GCTTATTTCC TCTGCAAAGA AAGAGTGAAG CAAAGCCTGG ATCAGATAGA TGAAGTTGCT 
GCTCTCATTG CTGCCACAGT CCATGATGTG GATCACCCTG GTCGGACAAA CTCGTTCCTT 
TGTAATGCAG GGAGTGAGCT GGCAATTCTG TACAACGACA CAGCAGTTTT AGAAAGTCAC 
CATGCAGCCC TAGCCTTCCA GCTGACCACT AGAGATGACA AGTGCAATAT ATTCAAAAAC 
ATGGAGAGGA ATGAATATCG CACTTTGCGG CAAGCAATCA TCGACATGGT CTTAGCAACT 
GAAATGACGA AGCACTTTGA ACATGTAAAC AAGTTTGTCA ACAGCATCAA TAAACCATTG 
GCAGCAGTGG AGGAGAATGG GGGCAGCATT GAGGAAGATG AGGCTGTTAA AAACATACTG 
ACATCTCCAG AAAACCGGAT CCTCATTAAA AGAATGCTTA TTAAGTGCGC AGACATTTCC 
AATCCGTGCC GGGCCCTCGA GCAGTGCATT GAGTGGGCTG GCCGGATATC AGAAGAGTAT 
TTTGCACAAA CAGATGAGGA GAAGAGAAAA GGTTTGCCTG TGGTGATGCC GGTGTTTGAC 
AGAAACACAT GTAGCATTCC GAAATCCCAG ATCTCCTTTG TTGATTACTT CATCACTGAT 
ATGTTCGATG CCTGGGATGC ATTTGCTGAT TTGCCCAACT TGATGCAACA TCTGGATAAT 
AACTTTAAAT ATTGGAAATC ACTAGACGAG AGACAGCTAA AGACTCTGCG ACCGCCCCCT 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755069.1
CDS	166..2571
Translation	MEWKKVKTLGGSSRGHNGPLNQAAVREVQFGPMKLRQDQLQVLLVFAKEDSQSNGFCWACEKAGFKCNIARTPETALESFLDKHHEIIIIDHRHSRYFDAEALCRSIRATKPSENTVIVAVVRRQSDRDEASIMPLISAGFTRRYVENSNIMACYNELIQLEFGEVLAQFKLRACNSLFTTLEKSQEAIEITSEDHIIQYVNPAFESTMGYQKDELIGKELTEVPINEKKSDFLDTINCCIKTGKEWEGIYYAKNKNGDNIQQNVKIIPVFGHRGKIRHYVSISRPCSDNNKAEKQCERVQAESQTDIQTCRHKDRRKGSLDVRSTTSRGSDGPAEFSHGALPGSSQRRHSSMARIHSMTIEAPITKVINIINAAQENSPSPVAEALDRVLEILRTTELYSPQLGTKDEDPHTNDLVGGLMTDGLRRLSGNEYILSAKQVHPVSGHMILALSLSDIPPRIAQAMDNEEDWEFDIFELEAATHKRPLVYLGLKMFARFGVCQFLNCSEATLRSWLHVIESNYHSSNAYHNSTHSADVLHATAYFLCKERVKQSLDQIDEVAALIAATVHDVDHPGRTNSFLCNAGSELAILYNDTAVLESHHAALAFQLTTRDDKCNIFKNMERNEYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPLAAVEENGGSIEEDEAVKNILTSPENRILIKRMLIKCADISNPCRALEQCIEWAGRISEEYFAQTDEEKRKGLPVVMPVFDRNTCSIPKSQISFVDYFITDMFDAWDAFADLPNLMQHLDNNFKYWKSLDERQLKTLRPPPE

Target ORF information:

RefSeq Version	XM_031899209.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 8A (pde8a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899209.1

ORF Insert Sequence:

ATGGAGTGGA AGAAGGTGAA AACTTTAGGA GGAAGCAGCA GAGGACACAA TGGTCCTTTA 
AATCAGGCAG CTGTAAGAGA AGTGCAGTTT GGTCCAATGA AACTCCGCCA AGATCAACTT 
CAGGTACTTT TAGTATTTGC CAAAGAAGAT AGCCAGAGTA ATGGATTCTG TTGGGCGTGT 
GAGAAAGCAG GCTTTAAGTG TAACATTGCC AGGACGCCGG AGACTGCACT GGAAAGCTTT 
TTAGATAAAC ATCACGAAAT TATCATTATT GATCATAGGC ATTCACGATA TTTTGATGCA 
GAAGCTCTAT GCAGGTCCAT TAGAGCTACG AAACCCTCAG AAAACACTGT CATAGTTGCT 
GTAGTTAGAA GACAAAGTGA TCGGGATGAA GCATCTATTA TGCCCCTAAT ATCTGCTGGA 
TTTACCAGGA GATATGTAGA AAACTCCAAT ATTATGGCCT GTTACAATGA GCTGATCCAG 
TTGGAATTTG GAGAAGTACT TGCACAATTT AAACTAAGGG CTTGTAATTC GTTATTTACT 
ACATTAGAAA AAAGTCAAGA AGCCATTGAA ATTACAAGTG AGGATCATAT AATACAGTAT 
GTGAATCCTG CTTTTGAAAG CACGATGGGT TATCAGAAGG ACGAACTCAT TGGGAAAGAA 
TTAACTGAAG TGCCTATTAA TGAAAAAAAA TCTGATTTCT TAGATACCAT TAATTGTTGC 
ATTAAAACGG GAAAGGAATG GGAAGGTATT TACTATGCCA AAAATAAAAA TGGTGACAAT 
ATCCAGCAAA ATGTGAAGAT AATTCCTGTT TTTGGACATC GGGGTAAAAT CAGACACTAT 
GTGTCTATAA GCCGTCCATG CAGTGACAAC AACAAGGCAG AAAAACAATG TGAGAGAGTT 
CAGGCCGAAT CGCAAACAGA TATCCAGACC TGTAGACACA AGGACAGGCG GAAAGGTTCC 
TTAGACGTCC GATCAACCAC CTCCAGAGGA AGCGATGGTC CAGCTGAATT TTCACACGGT 
GCTCTACCTG GTAGTTCACA GAGACGGCAT TCTTCTATGG CCAGAATACA TTCTATGACC 
ATTGAAGCCC CTATCACAAA GGTAATAAAT ATAATAAATG CTGCACAAGA AAACAGCCCT 
TCGCCTGTGG CAGAAGCATT AGATCGAGTT CTAGAGATTC TGAGGACCAC AGAACTATAC 
TCTCCGCAGC TGGGGACGAA AGATGAAGAC CCTCACACCA ACGACCTGGT CGGAGGTTTG 
ATGACAGATG GTTTACGAAG ACTGTCAGGA AATGAGTACA TCTTATCAGC TAAACAGGTA 
CACCCTGTCA GTGGGCATAT GATCCTAGCT CTTTCCCTAA GCGATATCCC ACCTCGGATA 
GCACAAGCAA TGGATAATGA AGAAGACTGG GAGTTCGATA TCTTTGAATT AGAGGCTGCT 
ACACATAAAA GGCCCTTAGT GTATCTTGGC CTTAAAATGT TTGCTCGGTT TGGTGTTTGT 
CAGTTTCTAA ACTGCTCAGA AGCTACTTTG AGGTCTTGGC TACATGTTAT CGAGTCAAAC 
TACCATTCAA GCAACGCCTA TCATAATTCA ACGCATTCCG CTGATGTCTT ACATGCCACT 
GCTTATTTCC TCTGCAAAGA AAGAGTGAAG CAAAGCCTGG ATCAGATAGA TGAAGTTGCT 
GCTCTCATTG CTGCCACAGT CCATGATGTG GATCACCCTG GTCGGACAAA CTCGTTCCTT 
TGTAATGCAG GGAGTGAGCT GGCAATTCTG TACAACGACA CAGCAGTTTT AGAAAGTCAC 
CATGCAGCCC TAGCCTTCCA GCTGACCACT AGAGATGACA AGTGCAATAT ATTCAAAAAC 
ATGGAGAGGA ATGAATATCG CACTTTGCGG CAAGCAATCA TCGACATGGT CTTAGCAACT 
GAAATGACGA AGCACTTTGA ACATGTAAAC AAGTTTGTCA ACAGCATCAA TAAACCATTG 
GCAGCAGTGG AGGAGAATGG GGGCAGCATT GAGGAAGATG AGGCTGTTAA AAACATACTG 
ACATCTCCAG AAAACCGGAT CCTCATTAAA AGAATGCTTA TTAAGTGCGC AGACATTTCC 
AATCCGTGCC GGGCCCTCGA GCAGTGCATT GAGTGGGCTG GCCGGATATC AGAAGAGTAT 
TTTGCACAAA CAGATGAGGA GAAGAGAAAA GGTTTGCCTG TGGTGATGCC GGTGTTTGAC 
AGAAACACAT GTAGCATTCC GAAATCCCAG ATCTCCTTTG TTGATTACTT CATCACTGAT 
ATGTTCGATG CCTGGGATGC ATTTGCTGAT TTGCCCAACT TGATGCAACA TCTGGATAAT 
AACTTTAAAT ATTGGAAATC ACTAGACGAG AGACAGCTAA AGACTCTGCG ACCGCCCCCT 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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pde8a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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