mtif2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	mtif2
Entrez Gene ID	100496951
Full Name	mitochondrial translational initiation factor 2
General protein information	Preferred Names mitochondrial translational initiation factor 2 Names translation initiation factor IF-2, mitochondrial
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002936912.4	XP_002936958.1	translation initiation factor IF-2, mitochondrial isoform X2
XM_004914739.3	XP_004914796.1	translation initiation factor IF-2, mitochondrial isoform X1
XM_004914740.3	XP_004914797.1	translation initiation factor IF-2, mitochondrial isoform X2
XM_004914739.4	XP_004914796.1	translation initiation factor IF-2, mitochondrial isoform X1
XM_002936912.5	XP_002936958.1	translation initiation factor IF-2, mitochondrial isoform X2
XM_004914740.4	XP_004914797.1	translation initiation factor IF-2, mitochondrial isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MTIF2	NP_001186353.1
Drosophila melanogaster (fruit fly)	CG12413	NP_651621.2
Magnaporthe oryzae (rice blast fungus)	MGG_11482	XP_003718118.1
Arabidopsis thaliana (thale cress)	AT4G11160	NP_192854.2
Danio rerio (zebrafish)	mtif2	NP_001082793.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001670	XP_321426.4
Oryza sativa (rice)	Os09g0515500	NP_001063668.1
Pan troglodytes (chimpanzee)	MTIF2	XP_001155358.1
Bos taurus (cattle)	MTIF2	NP_776818.1
Caenorhabditis elegans (roundworm)	F46B6.6	NP_001023936.1
Xenopus tropicalis (tropical clawed frog)	mtif2	XP_002936958.1
Homo sapiens (human)	MTIF2	NP_001005369.1
Mus musculus (house mouse)	Mtif2	NP_598528.2
	LOC717042	XP_002808083.1
Canis lupus familiaris (dog)	MTIF2	XP_531830.2
Rattus norvegicus (Norway rat)	Mtif2	NP_001004254.1

Gene Ontology

The following mtif2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mtif2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa10836	XM_004914739.4 Latest version!	Xenopus tropicalis mitochondrial translational initiation factor 2 (mtif2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa10837	XM_004914740.4 Latest version!	Xenopus tropicalis mitochondrial translational initiation factor 2 (mtif2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa10837	XM_002936912.5 Latest version!	Xenopus tropicalis mitochondrial translational initiation factor 2 (mtif2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OXa10836	XM_004914739.3	Xenopus tropicalis mitochondrial translational initiation factor 2 (mtif2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa10837	XM_002936912.4	Xenopus tropicalis mitochondrial translational initiation factor 2 (mtif2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa10837	XM_004914740.3	Xenopus tropicalis mitochondrial translational initiation factor 2 (mtif2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mtif2 ( XM_004914739.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914796.1 Xenopus tropicalis mitochondrial translational initiation factor 2 (mtif2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa10836
Clone ID Related Accession (Same CDS sequence)	XM_004914739.3 , XM_004914739.4
Accession Version	XM_004914739.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	translation initiation factor IF-2, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914739.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTA ACTGCGTTAC CGTCTGTCCC AAGCGTTTGC CGAAAATGAA CAGGAATGTA 
TTCATGCAGT TGGAACAGAT GGTTCGACTT CATAACACCT GCAGGAAATT GCATGCCCTG 
TGCCACACCA ATGCCAGGAT AAAATGGAGG CTTGTGCAGT GCCCTCCCTT AGCTGTATGT 
GTTGCCCACT TGTGCCCACC TTCCTGGCAA ACAGACACCT TCATCAGTGT GGCACTGTCA 
CAGTATCGTT GTTTGTCCTC AAAAGAGGGC AAATCTTTTA AAAAGCACAA GTTGTCGCCG 
ATCAAACCTA AGCAAGAAAA GCAGGAAGTG GAGATATGGA ACAATATGAC CGTTGGTGAT 
CTTGCAAGAG CCATGAATAA GGATACAGAA CACGTGTACG AAGCCCTTCT ACATACAGAT 
CTTGATTTGG AAGCTTTAGA AGAAAAGTCT GTCCTTAGCG AGAAGTGGAT AAAGGAAGCA 
ATTAAGAAAT CTGGCATGAA GTTTAAATAT GCAAAGATAA AAGAGGAAAA AGTCAGAGAA 
AACAAGGACG CGGTGAAAAG AGTCCCTGCA GATCCAGCTG TACTGATTCC CAGACCCCCT 
GTAATTACCA TAATGGGGCA TGTTGACCAT GGGAAAACGA CCCTGCTTGA CCAGCTAAGA 
AAAACCCAAG TGGCTGCAAT GGAAGCAGGG GGTATAACCC AGCACATTGG TGCATTTAAC 
GTTCAGTTAC CTTCAGGGGA GAGGATAACC TTTTTGGATA CTCCAGGACA TGCAGCCTTT 
TCAGCAATCC GAGAAAGAGG AGCTAATGTC ACTGATATTG TTATACTGGT GGTGGCGGCA 
GAGGATGGCG TTATGAAACA AACCGTTGAG TCCATACAAC ATGCCAAGAA TGCAAAAGTC 
CCTATTATCC TTGCCATAAA TAAATGTGAC AAACCAGAAG CCAGTCCCGA GAAAGTAAAG 
AAGGAGCTGC TGTCCCATGA TATTGTGTGT GAGGAGTATG GAGGGGAAAT CCAGGCTATT 
CACATCTCTG CACTAAAGGG ACACAACTTG CAGGCATTAA CAGAAGCCAC AGTCACCTTA 
GCAGAAATGT TGGAGCTAAA AGCAGACCCC ACAGGTCTAG TGGAGGGAAC TGTTGTAGAG 
TGTCGTACAG ACAAAGGGAA AGGTCCAGTG ACCACAGCCA TAGTGCAGAG AGGAACTCTT 
AAAAAGGGTG CTGTGTTGGT AGCAGGTAAA ACCTGGGCAA AGGTTCGCCT AATGTTTGAT 
GAGAACAACA AGGCTTTAAG CCAGGCGGGC CCTAGCACAC CTGTGGAGAT TGTTGGCTGG 
AAAGATCTAC CATCTGCGGG GGATGAAATT CTGGAAGTTG AGACAGAGCA ACGGGCTCGG 
GAAGTGGTGG ACTGGAGGAA ATACGTGGAA GAGCAAGAGA AAATAATAGG GGATATGGAG 
GTAATTGAGG CCAAGCAGAA GGAACACCGA GATGCCTACA AGAAGAACCT GGAGACTTTT 
AGCAACTTGA ACTGGAGACA AAGAAAATCT GCCATGTACA AAGCCAACAA GCAGCTTATG 
GCCACGAGGC CTGGCGAGAA GTCAGAGAGC GAAACACCCA TGTTCCCCCT TATTATAAAA 
GGAGATGTTG ATGGGTCAGT AGAAGCCATT CTCAGTATTA TAGACACTTA TGATGCCGAA 
GACCAGTGTG AGCTTGATTT AGTGCACTTT GGAGTTGGGG ATATCTCTGA AAATGACATT 
GCGCTCGCTG AGACATTCAA AGGCTATGTA TATGGGTTCA ATGTGAATTC AAACAAAGCG 
GTTCAGCAAC TGGCGGCCAA AAAAGAGGTT ACAGTGAAAC TGCATAAAGT CATCTACCAT 
CTGGTGGAAG ATCTGAGAGA AGAACTCAGC AAACAGCTGC CTCCGGTAAT TGAAGAAAAT 
ATAAAAGGTG AGGCATCTGT GTTGGCGACA TTTGATGTCA CAGTTGGGAA GAAGAAGGTG 
CAGGTGGCAG GCTGCAGGGT ACAGAAAGGC ATTTTGGATA AGAAGGTGAA ATGTAAAGTG 
ATCCGAAACA GAAAGATGAT TTGGGAAGGA TATTTAACGT CACTTAAACA CCTCAAGGAC 
GATGTGCAGA CAGTAAAAAC CGGGGTGGAG TGCGGCCTGA GTCTGGATAA AGACATCGAT 
GTAAAAATTG GCGACGAAGT TCTGTGTGTT GAAGAAAAGG AAGTTCAGCA GAAAATCTCC 
TGGGATCCTG GATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914796.1
CDS	82..2319
Translation	MASNCVTVCPKRLPKMNRNVFMQLEQMVRLHNTCRKLHALCHTNARIKWRLVQCPPLAVCVAHLCPPSWQTDTFISVALSQYRCLSSKEGKSFKKHKLSPIKPKQEKQEVEIWNNMTVGDLARAMNKDTEHVYEALLHTDLDLEALEEKSVLSEKWIKEAIKKSGMKFKYAKIKEEKVRENKDAVKRVPADPAVLIPRPPVITIMGHVDHGKTTLLDQLRKTQVAAMEAGGITQHIGAFNVQLPSGERITFLDTPGHAAFSAIRERGANVTDIVILVVAAEDGVMKQTVESIQHAKNAKVPIILAINKCDKPEASPEKVKKELLSHDIVCEEYGGEIQAIHISALKGHNLQALTEATVTLAEMLELKADPTGLVEGTVVECRTDKGKGPVTTAIVQRGTLKKGAVLVAGKTWAKVRLMFDENNKALSQAGPSTPVEIVGWKDLPSAGDEILEVETEQRAREVVDWRKYVEEQEKIIGDMEVIEAKQKEHRDAYKKNLETFSNLNWRQRKSAMYKANKQLMATRPGEKSESETPMFPLIIKGDVDGSVEAILSIIDTYDAEDQCELDLVHFGVGDISENDIALAETFKGYVYGFNVNSNKAVQQLAAKKEVTVKLHKVIYHLVEDLREELSKQLPPVIEENIKGEASVLATFDVTVGKKKVQVAGCRVQKGILDKKVKCKVIRNRKMIWEGYLTSLKHLKDDVQTVKTGVECGLSLDKDIDVKIGDEVLCVEEKEVQQKISWDPGF

Target ORF information:

RefSeq Version	XM_004914739.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitochondrial translational initiation factor 2 (mtif2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914739.3

ORF Insert Sequence:

ATGGCTTCTA ACTGCGTTAC CGTCTGTCCC AAGCGTTTGC CGAAAATGAA CAGGAATGTA 
TTCATGCAGT TGGAACAGAT GGTTCGACTT CATAACACCT GCAGGAAATT GCATGCCCTG 
TGCCACACCA ATGCCAGGAT AAAATGGAGG CTTGTGCAGT GCCCTCCCTT AGCTGTATGT 
GTTGCCCACT TGTGCCCACC TTCCTGGCAA ACAGACACCT TCATCAGTGT GGCACTGTCA 
CAGTATCGTT GTTTGTCCTC AAAAGAGGGC AAATCTTTTA AAAAGCACAA GTTGTCGCCG 
ATCAAACCTA AGCAAGAAAA GCAGGAAGTG GAGATATGGA ACAATATGAC CGTTGGTGAT 
CTTGCAAGAG CCATGAATAA GGATACAGAA CACGTGTACG AAGCCCTTCT ACATACAGAT 
CTTGATTTGG AAGCTTTAGA AGAAAAGTCT GTCCTTAGCG AGAAGTGGAT AAAGGAAGCA 
ATTAAGAAAT CTGGCATGAA GTTTAAATAT GCAAAGATAA AAGAGGAAAA AGTCAGAGAA 
AACAAGGACG CGGTGAAAAG AGTCCCTGCA GATCCAGCTG TACTGATTCC CAGACCCCCT 
GTAATTACCA TAATGGGGCA TGTTGACCAT GGGAAAACGA CCCTGCTTGA CCAGCTAAGA 
AAAACCCAAG TGGCTGCAAT GGAAGCAGGG GGTATAACCC AGCACATTGG TGCATTTAAC 
GTTCAGTTAC CTTCAGGGGA GAGGATAACC TTTTTGGATA CTCCAGGACA TGCAGCCTTT 
TCAGCAATCC GAGAAAGAGG AGCTAATGTC ACTGATATTG TTATACTGGT GGTGGCGGCA 
GAGGATGGCG TTATGAAACA AACCGTTGAG TCCATACAAC ATGCCAAGAA TGCAAAAGTC 
CCTATTATCC TTGCCATAAA TAAATGTGAC AAACCAGAAG CCAGTCCCGA GAAAGTAAAG 
AAGGAGCTGC TGTCCCATGA TATTGTGTGT GAGGAGTATG GAGGGGAAAT CCAGGCTATT 
CACATCTCTG CACTAAAGGG ACACAACTTG CAGGCATTAA CAGAAGCCAC AGTCACCTTA 
GCAGAAATGT TGGAGCTAAA AGCAGACCCC ACAGGTCTAG TGGAGGGAAC TGTTGTAGAG 
TGTCGTACAG ACAAAGGGAA AGGTCCAGTG ACCACAGCCA TAGTGCAGAG AGGAACTCTT 
AAAAAGGGTG CTGTGTTGGT AGCAGGTAAA ACCTGGGCAA AGGTTCGCCT AATGTTTGAT 
GAGAACAACA AGGCTTTAAG CCAGGCGGGC CCTAGCACAC CTGTGGAGAT TGTTGGCTGG 
AAAGATCTAC CATCTGCGGG GGATGAAATT CTGGAAGTTG AGACAGAGCA ACGGGCTCGG 
GAAGTGGTGG ACTGGAGGAA ATACGTGGAA GAGCAAGAGA AAATAATAGG GGATATGGAG 
GTAATTGAGG CCAAGCAGAA GGAACACCGA GATGCCTACA AGAAGAACCT GGAGACTTTT 
AGCAACTTGA ACTGGAGACA AAGAAAATCT GCCATGTACA AAGCCAACAA GCAGCTTATG 
GCCACGAGGC CTGGCGAGAA GTCAGAGAGC GAAACACCCA TGTTCCCCCT TATTATAAAA 
GGAGATGTTG ATGGGTCAGT AGAAGCCATT CTCAGTATTA TAGACACTTA TGATGCCGAA 
GACCAGTGTG AGCTTGATTT AGTGCACTTT GGAGTTGGGG ATATCTCTGA AAATGACATT 
GCGCTCGCTG AGACATTCAA AGGCTATGTA TATGGGTTCA ATGTGAATTC AAACAAAGCG 
GTTCAGCAAC TGGCGGCCAA AAAAGAGGTT ACAGTGAAAC TGCATAAAGT CATCTACCAT 
CTGGTGGAAG ATCTGAGAGA AGAACTCAGC AAACAGCTGC CTCCGGTAAT TGAAGAAAAT 
ATAAAAGGTG AGGCATCTGT GTTGGCGACA TTTGATGTCA CAGTTGGGAA GAAGAAGGTG 
CAGGTGGCAG GCTGCAGGGT ACAGAAAGGC ATTTTGGATA AGAAGGTGAA ATGTAAAGTG 
ATCCGAAACA GAAAGATGAT TTGGGAAGGA TATTTAACGT CACTTAAACA CCTCAAGGAC 
GATGTGCAGA CAGTAAAAAC CGGGGTGGAG TGCGGCCTGA GTCTGGATAA AGACATCGAT 
GTAAAAATTG GCGACGAAGT TCTGTGTGTT GAAGAAAAGG AAGTTCAGCA GAAAATCTCC 
TGGGATCCTG GATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mtif2 ( XM_002936912.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936958.1 Xenopus tropicalis mitochondrial translational initiation factor 2 (mtif2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa10837
Clone ID Related Accession (Same CDS sequence)	XM_002936912.4 , XM_004914740.3 , XM_002936912.5 , XM_004914740.4
Accession Version	XM_002936912.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	translation initiation factor IF-2, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002936912.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAGGA ATGTATTCAT GCAGTTGGAA CAGATGGTTC GACTTCATAA CACCTGCAGG 
AAATTGCATG CCCTGTGCCA CACCAATGCC AGGATAAAAT GGAGGCTTGT GCAGTGCCCT 
CCCTTAGCTG TATGTGTTGC CCACTTGTGC CCACCTTCCT GGCAAACAGA CACCTTCATC 
AGTGTGGCAC TGTCACAGTA TCGTTGTTTG TCCTCAAAAG AGGGCAAATC TTTTAAAAAG 
CACAAGTTGT CGCCGATCAA ACCTAAGCAA GAAAAGCAGG AAGTGGAGAT ATGGAACAAT 
ATGACCGTTG GTGATCTTGC AAGAGCCATG AATAAGGATA CAGAACACGT GTACGAAGCC 
CTTCTACATA CAGATCTTGA TTTGGAAGCT TTAGAAGAAA AGTCTGTCCT TAGCGAGAAG 
TGGATAAAGG AAGCAATTAA GAAATCTGGC ATGAAGTTTA AATATGCAAA GATAAAAGAG 
GAAAAAGTCA GAGAAAACAA GGACGCGGTG AAAAGAGTCC CTGCAGATCC AGCTGTACTG 
ATTCCCAGAC CCCCTGTAAT TACCATAATG GGGCATGTTG ACCATGGGAA AACGACCCTG 
CTTGACCAGC TAAGAAAAAC CCAAGTGGCT GCAATGGAAG CAGGGGGTAT AACCCAGCAC 
ATTGGTGCAT TTAACGTTCA GTTACCTTCA GGGGAGAGGA TAACCTTTTT GGATACTCCA 
GGACATGCAG CCTTTTCAGC AATCCGAGAA AGAGGAGCTA ATGTCACTGA TATTGTTATA 
CTGGTGGTGG CGGCAGAGGA TGGCGTTATG AAACAAACCG TTGAGTCCAT ACAACATGCC 
AAGAATGCAA AAGTCCCTAT TATCCTTGCC ATAAATAAAT GTGACAAACC AGAAGCCAGT 
CCCGAGAAAG TAAAGAAGGA GCTGCTGTCC CATGATATTG TGTGTGAGGA GTATGGAGGG 
GAAATCCAGG CTATTCACAT CTCTGCACTA AAGGGACACA ACTTGCAGGC ATTAACAGAA 
GCCACAGTCA CCTTAGCAGA AATGTTGGAG CTAAAAGCAG ACCCCACAGG TCTAGTGGAG 
GGAACTGTTG TAGAGTGTCG TACAGACAAA GGGAAAGGTC CAGTGACCAC AGCCATAGTG 
CAGAGAGGAA CTCTTAAAAA GGGTGCTGTG TTGGTAGCAG GTAAAACCTG GGCAAAGGTT 
CGCCTAATGT TTGATGAGAA CAACAAGGCT TTAAGCCAGG CGGGCCCTAG CACACCTGTG 
GAGATTGTTG GCTGGAAAGA TCTACCATCT GCGGGGGATG AAATTCTGGA AGTTGAGACA 
GAGCAACGGG CTCGGGAAGT GGTGGACTGG AGGAAATACG TGGAAGAGCA AGAGAAAATA 
ATAGGGGATA TGGAGGTAAT TGAGGCCAAG CAGAAGGAAC ACCGAGATGC CTACAAGAAG 
AACCTGGAGA CTTTTAGCAA CTTGAACTGG AGACAAAGAA AATCTGCCAT GTACAAAGCC 
AACAAGCAGC TTATGGCCAC GAGGCCTGGC GAGAAGTCAG AGAGCGAAAC ACCCATGTTC 
CCCCTTATTA TAAAAGGAGA TGTTGATGGG TCAGTAGAAG CCATTCTCAG TATTATAGAC 
ACTTATGATG CCGAAGACCA GTGTGAGCTT GATTTAGTGC ACTTTGGAGT TGGGGATATC 
TCTGAAAATG ACATTGCGCT CGCTGAGACA TTCAAAGGCT ATGTATATGG GTTCAATGTG 
AATTCAAACA AAGCGGTTCA GCAACTGGCG GCCAAAAAAG AGGTTACAGT GAAACTGCAT 
AAAGTCATCT ACCATCTGGT GGAAGATCTG AGAGAAGAAC TCAGCAAACA GCTGCCTCCG 
GTAATTGAAG AAAATATAAA AGGTGAGGCA TCTGTGTTGG CGACATTTGA TGTCACAGTT 
GGGAAGAAGA AGGTGCAGGT GGCAGGCTGC AGGGTACAGA AAGGCATTTT GGATAAGAAG 
GTGAAATGTA AAGTGATCCG AAACAGAAAG ATGATTTGGG AAGGATATTT AACGTCACTT 
AAACACCTCA AGGACGATGT GCAGACAGTA AAAACCGGGG TGGAGTGCGG CCTGAGTCTG 
GATAAAGACA TCGATGTAAA AATTGGCGAC GAAGTTCTGT GTGTTGAAGA AAAGGAAGTT 
CAGCAGAAAA TCTCCTGGGA TCCTGGATTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936958.1
CDS	229..2421
Translation	MNRNVFMQLEQMVRLHNTCRKLHALCHTNARIKWRLVQCPPLAVCVAHLCPPSWQTDTFISVALSQYRCLSSKEGKSFKKHKLSPIKPKQEKQEVEIWNNMTVGDLARAMNKDTEHVYEALLHTDLDLEALEEKSVLSEKWIKEAIKKSGMKFKYAKIKEEKVRENKDAVKRVPADPAVLIPRPPVITIMGHVDHGKTTLLDQLRKTQVAAMEAGGITQHIGAFNVQLPSGERITFLDTPGHAAFSAIRERGANVTDIVILVVAAEDGVMKQTVESIQHAKNAKVPIILAINKCDKPEASPEKVKKELLSHDIVCEEYGGEIQAIHISALKGHNLQALTEATVTLAEMLELKADPTGLVEGTVVECRTDKGKGPVTTAIVQRGTLKKGAVLVAGKTWAKVRLMFDENNKALSQAGPSTPVEIVGWKDLPSAGDEILEVETEQRAREVVDWRKYVEEQEKIIGDMEVIEAKQKEHRDAYKKNLETFSNLNWRQRKSAMYKANKQLMATRPGEKSESETPMFPLIIKGDVDGSVEAILSIIDTYDAEDQCELDLVHFGVGDISENDIALAETFKGYVYGFNVNSNKAVQQLAAKKEVTVKLHKVIYHLVEDLREELSKQLPPVIEENIKGEASVLATFDVTVGKKKVQVAGCRVQKGILDKKVKCKVIRNRKMIWEGYLTSLKHLKDDVQTVKTGVECGLSLDKDIDVKIGDEVLCVEEKEVQQKISWDPGF

Target ORF information:

RefSeq Version	XM_002936912.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitochondrial translational initiation factor 2 (mtif2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936912.4

ORF Insert Sequence:

ATGAACAGGA ATGTATTCAT GCAGTTGGAA CAGATGGTTC GACTTCATAA CACCTGCAGG 
AAATTGCATG CCCTGTGCCA CACCAATGCC AGGATAAAAT GGAGGCTTGT GCAGTGCCCT 
CCCTTAGCTG TATGTGTTGC CCACTTGTGC CCACCTTCCT GGCAAACAGA CACCTTCATC 
AGTGTGGCAC TGTCACAGTA TCGTTGTTTG TCCTCAAAAG AGGGCAAATC TTTTAAAAAG 
CACAAGTTGT CGCCGATCAA ACCTAAGCAA GAAAAGCAGG AAGTGGAGAT ATGGAACAAT 
ATGACCGTTG GTGATCTTGC AAGAGCCATG AATAAGGATA CAGAACACGT GTACGAAGCC 
CTTCTACATA CAGATCTTGA TTTGGAAGCT TTAGAAGAAA AGTCTGTCCT TAGCGAGAAG 
TGGATAAAGG AAGCAATTAA GAAATCTGGC ATGAAGTTTA AATATGCAAA GATAAAAGAG 
GAAAAAGTCA GAGAAAACAA GGACGCGGTG AAAAGAGTCC CTGCAGATCC AGCTGTACTG 
ATTCCCAGAC CCCCTGTAAT TACCATAATG GGGCATGTTG ACCATGGGAA AACGACCCTG 
CTTGACCAGC TAAGAAAAAC CCAAGTGGCT GCAATGGAAG CAGGGGGTAT AACCCAGCAC 
ATTGGTGCAT TTAACGTTCA GTTACCTTCA GGGGAGAGGA TAACCTTTTT GGATACTCCA 
GGACATGCAG CCTTTTCAGC AATCCGAGAA AGAGGAGCTA ATGTCACTGA TATTGTTATA 
CTGGTGGTGG CGGCAGAGGA TGGCGTTATG AAACAAACCG TTGAGTCCAT ACAACATGCC 
AAGAATGCAA AAGTCCCTAT TATCCTTGCC ATAAATAAAT GTGACAAACC AGAAGCCAGT 
CCCGAGAAAG TAAAGAAGGA GCTGCTGTCC CATGATATTG TGTGTGAGGA GTATGGAGGG 
GAAATCCAGG CTATTCACAT CTCTGCACTA AAGGGACACA ACTTGCAGGC ATTAACAGAA 
GCCACAGTCA CCTTAGCAGA AATGTTGGAG CTAAAAGCAG ACCCCACAGG TCTAGTGGAG 
GGAACTGTTG TAGAGTGTCG TACAGACAAA GGGAAAGGTC CAGTGACCAC AGCCATAGTG 
CAGAGAGGAA CTCTTAAAAA GGGTGCTGTG TTGGTAGCAG GTAAAACCTG GGCAAAGGTT 
CGCCTAATGT TTGATGAGAA CAACAAGGCT TTAAGCCAGG CGGGCCCTAG CACACCTGTG 
GAGATTGTTG GCTGGAAAGA TCTACCATCT GCGGGGGATG AAATTCTGGA AGTTGAGACA 
GAGCAACGGG CTCGGGAAGT GGTGGACTGG AGGAAATACG TGGAAGAGCA AGAGAAAATA 
ATAGGGGATA TGGAGGTAAT TGAGGCCAAG CAGAAGGAAC ACCGAGATGC CTACAAGAAG 
AACCTGGAGA CTTTTAGCAA CTTGAACTGG AGACAAAGAA AATCTGCCAT GTACAAAGCC 
AACAAGCAGC TTATGGCCAC GAGGCCTGGC GAGAAGTCAG AGAGCGAAAC ACCCATGTTC 
CCCCTTATTA TAAAAGGAGA TGTTGATGGG TCAGTAGAAG CCATTCTCAG TATTATAGAC 
ACTTATGATG CCGAAGACCA GTGTGAGCTT GATTTAGTGC ACTTTGGAGT TGGGGATATC 
TCTGAAAATG ACATTGCGCT CGCTGAGACA TTCAAAGGCT ATGTATATGG GTTCAATGTG 
AATTCAAACA AAGCGGTTCA GCAACTGGCG GCCAAAAAAG AGGTTACAGT GAAACTGCAT 
AAAGTCATCT ACCATCTGGT GGAAGATCTG AGAGAAGAAC TCAGCAAACA GCTGCCTCCG 
GTAATTGAAG AAAATATAAA AGGTGAGGCA TCTGTGTTGG CGACATTTGA TGTCACAGTT 
GGGAAGAAGA AGGTGCAGGT GGCAGGCTGC AGGGTACAGA AAGGCATTTT GGATAAGAAG 
GTGAAATGTA AAGTGATCCG AAACAGAAAG ATGATTTGGG AAGGATATTT AACGTCACTT 
AAACACCTCA AGGACGATGT GCAGACAGTA AAAACCGGGG TGGAGTGCGG CCTGAGTCTG 
GATAAAGACA TCGATGTAAA AATTGGCGAC GAAGTTCTGT GTGTTGAAGA AAAGGAAGTT 
CAGCAGAAAA TCTCCTGGGA TCCTGGATTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mtif2 ( XM_004914740.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914797.1 Xenopus tropicalis mitochondrial translational initiation factor 2 (mtif2), transcript variant X3, mRNA.

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CloneID	OXa10837
Clone ID Related Accession (Same CDS sequence)	XM_002936912.4 , XM_004914740.3 , XM_002936912.5 , XM_004914740.4
Accession Version	XM_004914740.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	translation initiation factor IF-2, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914740.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAGGA ATGTATTCAT GCAGTTGGAA CAGATGGTTC GACTTCATAA CACCTGCAGG 
AAATTGCATG CCCTGTGCCA CACCAATGCC AGGATAAAAT GGAGGCTTGT GCAGTGCCCT 
CCCTTAGCTG TATGTGTTGC CCACTTGTGC CCACCTTCCT GGCAAACAGA CACCTTCATC 
AGTGTGGCAC TGTCACAGTA TCGTTGTTTG TCCTCAAAAG AGGGCAAATC TTTTAAAAAG 
CACAAGTTGT CGCCGATCAA ACCTAAGCAA GAAAAGCAGG AAGTGGAGAT ATGGAACAAT 
ATGACCGTTG GTGATCTTGC AAGAGCCATG AATAAGGATA CAGAACACGT GTACGAAGCC 
CTTCTACATA CAGATCTTGA TTTGGAAGCT TTAGAAGAAA AGTCTGTCCT TAGCGAGAAG 
TGGATAAAGG AAGCAATTAA GAAATCTGGC ATGAAGTTTA AATATGCAAA GATAAAAGAG 
GAAAAAGTCA GAGAAAACAA GGACGCGGTG AAAAGAGTCC CTGCAGATCC AGCTGTACTG 
ATTCCCAGAC CCCCTGTAAT TACCATAATG GGGCATGTTG ACCATGGGAA AACGACCCTG 
CTTGACCAGC TAAGAAAAAC CCAAGTGGCT GCAATGGAAG CAGGGGGTAT AACCCAGCAC 
ATTGGTGCAT TTAACGTTCA GTTACCTTCA GGGGAGAGGA TAACCTTTTT GGATACTCCA 
GGACATGCAG CCTTTTCAGC AATCCGAGAA AGAGGAGCTA ATGTCACTGA TATTGTTATA 
CTGGTGGTGG CGGCAGAGGA TGGCGTTATG AAACAAACCG TTGAGTCCAT ACAACATGCC 
AAGAATGCAA AAGTCCCTAT TATCCTTGCC ATAAATAAAT GTGACAAACC AGAAGCCAGT 
CCCGAGAAAG TAAAGAAGGA GCTGCTGTCC CATGATATTG TGTGTGAGGA GTATGGAGGG 
GAAATCCAGG CTATTCACAT CTCTGCACTA AAGGGACACA ACTTGCAGGC ATTAACAGAA 
GCCACAGTCA CCTTAGCAGA AATGTTGGAG CTAAAAGCAG ACCCCACAGG TCTAGTGGAG 
GGAACTGTTG TAGAGTGTCG TACAGACAAA GGGAAAGGTC CAGTGACCAC AGCCATAGTG 
CAGAGAGGAA CTCTTAAAAA GGGTGCTGTG TTGGTAGCAG GTAAAACCTG GGCAAAGGTT 
CGCCTAATGT TTGATGAGAA CAACAAGGCT TTAAGCCAGG CGGGCCCTAG CACACCTGTG 
GAGATTGTTG GCTGGAAAGA TCTACCATCT GCGGGGGATG AAATTCTGGA AGTTGAGACA 
GAGCAACGGG CTCGGGAAGT GGTGGACTGG AGGAAATACG TGGAAGAGCA AGAGAAAATA 
ATAGGGGATA TGGAGGTAAT TGAGGCCAAG CAGAAGGAAC ACCGAGATGC CTACAAGAAG 
AACCTGGAGA CTTTTAGCAA CTTGAACTGG AGACAAAGAA AATCTGCCAT GTACAAAGCC 
AACAAGCAGC TTATGGCCAC GAGGCCTGGC GAGAAGTCAG AGAGCGAAAC ACCCATGTTC 
CCCCTTATTA TAAAAGGAGA TGTTGATGGG TCAGTAGAAG CCATTCTCAG TATTATAGAC 
ACTTATGATG CCGAAGACCA GTGTGAGCTT GATTTAGTGC ACTTTGGAGT TGGGGATATC 
TCTGAAAATG ACATTGCGCT CGCTGAGACA TTCAAAGGCT ATGTATATGG GTTCAATGTG 
AATTCAAACA AAGCGGTTCA GCAACTGGCG GCCAAAAAAG AGGTTACAGT GAAACTGCAT 
AAAGTCATCT ACCATCTGGT GGAAGATCTG AGAGAAGAAC TCAGCAAACA GCTGCCTCCG 
GTAATTGAAG AAAATATAAA AGGTGAGGCA TCTGTGTTGG CGACATTTGA TGTCACAGTT 
GGGAAGAAGA AGGTGCAGGT GGCAGGCTGC AGGGTACAGA AAGGCATTTT GGATAAGAAG 
GTGAAATGTA AAGTGATCCG AAACAGAAAG ATGATTTGGG AAGGATATTT AACGTCACTT 
AAACACCTCA AGGACGATGT GCAGACAGTA AAAACCGGGG TGGAGTGCGG CCTGAGTCTG 
GATAAAGACA TCGATGTAAA AATTGGCGAC GAAGTTCTGT GTGTTGAAGA AAAGGAAGTT 
CAGCAGAAAA TCTCCTGGGA TCCTGGATTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914797.1
CDS	91..2283
Translation	MNRNVFMQLEQMVRLHNTCRKLHALCHTNARIKWRLVQCPPLAVCVAHLCPPSWQTDTFISVALSQYRCLSSKEGKSFKKHKLSPIKPKQEKQEVEIWNNMTVGDLARAMNKDTEHVYEALLHTDLDLEALEEKSVLSEKWIKEAIKKSGMKFKYAKIKEEKVRENKDAVKRVPADPAVLIPRPPVITIMGHVDHGKTTLLDQLRKTQVAAMEAGGITQHIGAFNVQLPSGERITFLDTPGHAAFSAIRERGANVTDIVILVVAAEDGVMKQTVESIQHAKNAKVPIILAINKCDKPEASPEKVKKELLSHDIVCEEYGGEIQAIHISALKGHNLQALTEATVTLAEMLELKADPTGLVEGTVVECRTDKGKGPVTTAIVQRGTLKKGAVLVAGKTWAKVRLMFDENNKALSQAGPSTPVEIVGWKDLPSAGDEILEVETEQRAREVVDWRKYVEEQEKIIGDMEVIEAKQKEHRDAYKKNLETFSNLNWRQRKSAMYKANKQLMATRPGEKSESETPMFPLIIKGDVDGSVEAILSIIDTYDAEDQCELDLVHFGVGDISENDIALAETFKGYVYGFNVNSNKAVQQLAAKKEVTVKLHKVIYHLVEDLREELSKQLPPVIEENIKGEASVLATFDVTVGKKKVQVAGCRVQKGILDKKVKCKVIRNRKMIWEGYLTSLKHLKDDVQTVKTGVECGLSLDKDIDVKIGDEVLCVEEKEVQQKISWDPGF

Target ORF information:

RefSeq Version	XM_004914740.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitochondrial translational initiation factor 2 (mtif2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914740.3

ORF Insert Sequence:

ATGAACAGGA ATGTATTCAT GCAGTTGGAA CAGATGGTTC GACTTCATAA CACCTGCAGG 
AAATTGCATG CCCTGTGCCA CACCAATGCC AGGATAAAAT GGAGGCTTGT GCAGTGCCCT 
CCCTTAGCTG TATGTGTTGC CCACTTGTGC CCACCTTCCT GGCAAACAGA CACCTTCATC 
AGTGTGGCAC TGTCACAGTA TCGTTGTTTG TCCTCAAAAG AGGGCAAATC TTTTAAAAAG 
CACAAGTTGT CGCCGATCAA ACCTAAGCAA GAAAAGCAGG AAGTGGAGAT ATGGAACAAT 
ATGACCGTTG GTGATCTTGC AAGAGCCATG AATAAGGATA CAGAACACGT GTACGAAGCC 
CTTCTACATA CAGATCTTGA TTTGGAAGCT TTAGAAGAAA AGTCTGTCCT TAGCGAGAAG 
TGGATAAAGG AAGCAATTAA GAAATCTGGC ATGAAGTTTA AATATGCAAA GATAAAAGAG 
GAAAAAGTCA GAGAAAACAA GGACGCGGTG AAAAGAGTCC CTGCAGATCC AGCTGTACTG 
ATTCCCAGAC CCCCTGTAAT TACCATAATG GGGCATGTTG ACCATGGGAA AACGACCCTG 
CTTGACCAGC TAAGAAAAAC CCAAGTGGCT GCAATGGAAG CAGGGGGTAT AACCCAGCAC 
ATTGGTGCAT TTAACGTTCA GTTACCTTCA GGGGAGAGGA TAACCTTTTT GGATACTCCA 
GGACATGCAG CCTTTTCAGC AATCCGAGAA AGAGGAGCTA ATGTCACTGA TATTGTTATA 
CTGGTGGTGG CGGCAGAGGA TGGCGTTATG AAACAAACCG TTGAGTCCAT ACAACATGCC 
AAGAATGCAA AAGTCCCTAT TATCCTTGCC ATAAATAAAT GTGACAAACC AGAAGCCAGT 
CCCGAGAAAG TAAAGAAGGA GCTGCTGTCC CATGATATTG TGTGTGAGGA GTATGGAGGG 
GAAATCCAGG CTATTCACAT CTCTGCACTA AAGGGACACA ACTTGCAGGC ATTAACAGAA 
GCCACAGTCA CCTTAGCAGA AATGTTGGAG CTAAAAGCAG ACCCCACAGG TCTAGTGGAG 
GGAACTGTTG TAGAGTGTCG TACAGACAAA GGGAAAGGTC CAGTGACCAC AGCCATAGTG 
CAGAGAGGAA CTCTTAAAAA GGGTGCTGTG TTGGTAGCAG GTAAAACCTG GGCAAAGGTT 
CGCCTAATGT TTGATGAGAA CAACAAGGCT TTAAGCCAGG CGGGCCCTAG CACACCTGTG 
GAGATTGTTG GCTGGAAAGA TCTACCATCT GCGGGGGATG AAATTCTGGA AGTTGAGACA 
GAGCAACGGG CTCGGGAAGT GGTGGACTGG AGGAAATACG TGGAAGAGCA AGAGAAAATA 
ATAGGGGATA TGGAGGTAAT TGAGGCCAAG CAGAAGGAAC ACCGAGATGC CTACAAGAAG 
AACCTGGAGA CTTTTAGCAA CTTGAACTGG AGACAAAGAA AATCTGCCAT GTACAAAGCC 
AACAAGCAGC TTATGGCCAC GAGGCCTGGC GAGAAGTCAG AGAGCGAAAC ACCCATGTTC 
CCCCTTATTA TAAAAGGAGA TGTTGATGGG TCAGTAGAAG CCATTCTCAG TATTATAGAC 
ACTTATGATG CCGAAGACCA GTGTGAGCTT GATTTAGTGC ACTTTGGAGT TGGGGATATC 
TCTGAAAATG ACATTGCGCT CGCTGAGACA TTCAAAGGCT ATGTATATGG GTTCAATGTG 
AATTCAAACA AAGCGGTTCA GCAACTGGCG GCCAAAAAAG AGGTTACAGT GAAACTGCAT 
AAAGTCATCT ACCATCTGGT GGAAGATCTG AGAGAAGAAC TCAGCAAACA GCTGCCTCCG 
GTAATTGAAG AAAATATAAA AGGTGAGGCA TCTGTGTTGG CGACATTTGA TGTCACAGTT 
GGGAAGAAGA AGGTGCAGGT GGCAGGCTGC AGGGTACAGA AAGGCATTTT GGATAAGAAG 
GTGAAATGTA AAGTGATCCG AAACAGAAAG ATGATTTGGG AAGGATATTT AACGTCACTT 
AAACACCTCA AGGACGATGT GCAGACAGTA AAAACCGGGG TGGAGTGCGG CCTGAGTCTG 
GATAAAGACA TCGATGTAAA AATTGGCGAC GAAGTTCTGT GTGTTGAAGA AAAGGAAGTT 
CAGCAGAAAA TCTCCTGGGA TCCTGGATTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mtif2 ( XM_004914739.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914796.1 Xenopus tropicalis mitochondrial translational initiation factor 2 (mtif2), transcript variant X1, mRNA.

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CloneID	OXa10836
Clone ID Related Accession (Same CDS sequence)	XM_004914739.3 , XM_004914739.4
Accession Version	XM_004914739.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	translation initiation factor IF-2, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914739.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTA ACTGCGTTAC CGTCTGTCCC AAGCGTTTGC CGAAAATGAA CAGGAATGTA 
TTCATGCAGT TGGAACAGAT GGTTCGACTT CATAACACCT GCAGGAAATT GCATGCCCTG 
TGCCACACCA ATGCCAGGAT AAAATGGAGG CTTGTGCAGT GCCCTCCCTT AGCTGTATGT 
GTTGCCCACT TGTGCCCACC TTCCTGGCAA ACAGACACCT TCATCAGTGT GGCACTGTCA 
CAGTATCGTT GTTTGTCCTC AAAAGAGGGC AAATCTTTTA AAAAGCACAA GTTGTCGCCG 
ATCAAACCTA AGCAAGAAAA GCAGGAAGTG GAGATATGGA ACAATATGAC CGTTGGTGAT 
CTTGCAAGAG CCATGAATAA GGATACAGAA CACGTGTACG AAGCCCTTCT ACATACAGAT 
CTTGATTTGG AAGCTTTAGA AGAAAAGTCT GTCCTTAGCG AGAAGTGGAT AAAGGAAGCA 
ATTAAGAAAT CTGGCATGAA GTTTAAATAT GCAAAGATAA AAGAGGAAAA AGTCAGAGAA 
AACAAGGACG CGGTGAAAAG AGTCCCTGCA GATCCAGCTG TACTGATTCC CAGACCCCCT 
GTAATTACCA TAATGGGGCA TGTTGACCAT GGGAAAACGA CCCTGCTTGA CCAGCTAAGA 
AAAACCCAAG TGGCTGCAAT GGAAGCAGGG GGTATAACCC AGCACATTGG TGCATTTAAC 
GTTCAGTTAC CTTCAGGGGA GAGGATAACC TTTTTGGATA CTCCAGGACA TGCAGCCTTT 
TCAGCAATCC GAGAAAGAGG AGCTAATGTC ACTGATATTG TTATACTGGT GGTGGCGGCA 
GAGGATGGCG TTATGAAACA AACCGTTGAG TCCATACAAC ATGCCAAGAA TGCAAAAGTC 
CCTATTATCC TTGCCATAAA TAAATGTGAC AAACCAGAAG CCAGTCCCGA GAAAGTAAAG 
AAGGAGCTGC TGTCCCATGA TATTGTGTGT GAGGAGTATG GAGGGGAAAT CCAGGCTATT 
CACATCTCTG CACTAAAGGG ACACAACTTG CAGGCATTAA CAGAAGCCAC AGTCACCTTA 
GCAGAAATGT TGGAGCTAAA AGCAGACCCC ACAGGTCTAG TGGAGGGAAC TGTTGTAGAG 
TGTCGTACAG ACAAAGGGAA AGGTCCAGTG ACCACAGCCA TAGTGCAGAG AGGAACTCTT 
AAAAAGGGTG CTGTGTTGGT AGCAGGTAAA ACCTGGGCAA AGGTTCGCCT AATGTTTGAT 
GAGAACAACA AGGCTTTAAG CCAGGCGGGC CCTAGCACAC CTGTGGAGAT TGTTGGCTGG 
AAAGATCTAC CATCTGCGGG GGATGAAATT CTGGAAGTTG AGACAGAGCA ACGGGCTCGG 
GAAGTGGTGG ACTGGAGGAA ATACGTGGAA GAGCAAGAGA AAATAATAGG GGATATGGAG 
GTAATTGAGG CCAAGCAGAA GGAACACCGA GATGCCTACA AGAAGAACCT GGAGACTTTT 
AGCAACTTGA ACTGGAGACA AAGAAAATCT GCCATGTACA AAGCCAACAA GCAGCTTATG 
GCCACGAGGC CTGGCGAGAA GTCAGAGAGC GAAACACCCA TGTTCCCCCT TATTATAAAA 
GGAGATGTTG ATGGGTCAGT AGAAGCCATT CTCAGTATTA TAGACACTTA TGATGCCGAA 
GACCAGTGTG AGCTTGATTT AGTGCACTTT GGAGTTGGGG ATATCTCTGA AAATGACATT 
GCGCTCGCTG AGACATTCAA AGGCTATGTA TATGGGTTCA ATGTGAATTC AAACAAAGCG 
GTTCAGCAAC TGGCGGCCAA AAAAGAGGTT ACAGTGAAAC TGCATAAAGT CATCTACCAT 
CTGGTGGAAG ATCTGAGAGA AGAACTCAGC AAACAGCTGC CTCCGGTAAT TGAAGAAAAT 
ATAAAAGGTG AGGCATCTGT GTTGGCGACA TTTGATGTCA CAGTTGGGAA GAAGAAGGTG 
CAGGTGGCAG GCTGCAGGGT ACAGAAAGGC ATTTTGGATA AGAAGGTGAA ATGTAAAGTG 
ATCCGAAACA GAAAGATGAT TTGGGAAGGA TATTTAACGT CACTTAAACA CCTCAAGGAC 
GATGTGCAGA CAGTAAAAAC CGGGGTGGAG TGCGGCCTGA GTCTGGATAA AGACATCGAT 
GTAAAAATTG GCGACGAAGT TCTGTGTGTT GAAGAAAAGG AAGTTCAGCA GAAAATCTCC 
TGGGATCCTG GATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914796.1
CDS	82..2319
Translation	MASNCVTVCPKRLPKMNRNVFMQLEQMVRLHNTCRKLHALCHTNARIKWRLVQCPPLAVCVAHLCPPSWQTDTFISVALSQYRCLSSKEGKSFKKHKLSPIKPKQEKQEVEIWNNMTVGDLARAMNKDTEHVYEALLHTDLDLEALEEKSVLSEKWIKEAIKKSGMKFKYAKIKEEKVRENKDAVKRVPADPAVLIPRPPVITIMGHVDHGKTTLLDQLRKTQVAAMEAGGITQHIGAFNVQLPSGERITFLDTPGHAAFSAIRERGANVTDIVILVVAAEDGVMKQTVESIQHAKNAKVPIILAINKCDKPEASPEKVKKELLSHDIVCEEYGGEIQAIHISALKGHNLQALTEATVTLAEMLELKADPTGLVEGTVVECRTDKGKGPVTTAIVQRGTLKKGAVLVAGKTWAKVRLMFDENNKALSQAGPSTPVEIVGWKDLPSAGDEILEVETEQRAREVVDWRKYVEEQEKIIGDMEVIEAKQKEHRDAYKKNLETFSNLNWRQRKSAMYKANKQLMATRPGEKSESETPMFPLIIKGDVDGSVEAILSIIDTYDAEDQCELDLVHFGVGDISENDIALAETFKGYVYGFNVNSNKAVQQLAAKKEVTVKLHKVIYHLVEDLREELSKQLPPVIEENIKGEASVLATFDVTVGKKKVQVAGCRVQKGILDKKVKCKVIRNRKMIWEGYLTSLKHLKDDVQTVKTGVECGLSLDKDIDVKIGDEVLCVEEKEVQQKISWDPGF

Target ORF information:

RefSeq Version	XM_004914739.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitochondrial translational initiation factor 2 (mtif2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914739.4

ORF Insert Sequence:

ATGGCTTCTA ACTGCGTTAC CGTCTGTCCC AAGCGTTTGC CGAAAATGAA CAGGAATGTA 
TTCATGCAGT TGGAACAGAT GGTTCGACTT CATAACACCT GCAGGAAATT GCATGCCCTG 
TGCCACACCA ATGCCAGGAT AAAATGGAGG CTTGTGCAGT GCCCTCCCTT AGCTGTATGT 
GTTGCCCACT TGTGCCCACC TTCCTGGCAA ACAGACACCT TCATCAGTGT GGCACTGTCA 
CAGTATCGTT GTTTGTCCTC AAAAGAGGGC AAATCTTTTA AAAAGCACAA GTTGTCGCCG 
ATCAAACCTA AGCAAGAAAA GCAGGAAGTG GAGATATGGA ACAATATGAC CGTTGGTGAT 
CTTGCAAGAG CCATGAATAA GGATACAGAA CACGTGTACG AAGCCCTTCT ACATACAGAT 
CTTGATTTGG AAGCTTTAGA AGAAAAGTCT GTCCTTAGCG AGAAGTGGAT AAAGGAAGCA 
ATTAAGAAAT CTGGCATGAA GTTTAAATAT GCAAAGATAA AAGAGGAAAA AGTCAGAGAA 
AACAAGGACG CGGTGAAAAG AGTCCCTGCA GATCCAGCTG TACTGATTCC CAGACCCCCT 
GTAATTACCA TAATGGGGCA TGTTGACCAT GGGAAAACGA CCCTGCTTGA CCAGCTAAGA 
AAAACCCAAG TGGCTGCAAT GGAAGCAGGG GGTATAACCC AGCACATTGG TGCATTTAAC 
GTTCAGTTAC CTTCAGGGGA GAGGATAACC TTTTTGGATA CTCCAGGACA TGCAGCCTTT 
TCAGCAATCC GAGAAAGAGG AGCTAATGTC ACTGATATTG TTATACTGGT GGTGGCGGCA 
GAGGATGGCG TTATGAAACA AACCGTTGAG TCCATACAAC ATGCCAAGAA TGCAAAAGTC 
CCTATTATCC TTGCCATAAA TAAATGTGAC AAACCAGAAG CCAGTCCCGA GAAAGTAAAG 
AAGGAGCTGC TGTCCCATGA TATTGTGTGT GAGGAGTATG GAGGGGAAAT CCAGGCTATT 
CACATCTCTG CACTAAAGGG ACACAACTTG CAGGCATTAA CAGAAGCCAC AGTCACCTTA 
GCAGAAATGT TGGAGCTAAA AGCAGACCCC ACAGGTCTAG TGGAGGGAAC TGTTGTAGAG 
TGTCGTACAG ACAAAGGGAA AGGTCCAGTG ACCACAGCCA TAGTGCAGAG AGGAACTCTT 
AAAAAGGGTG CTGTGTTGGT AGCAGGTAAA ACCTGGGCAA AGGTTCGCCT AATGTTTGAT 
GAGAACAACA AGGCTTTAAG CCAGGCGGGC CCTAGCACAC CTGTGGAGAT TGTTGGCTGG 
AAAGATCTAC CATCTGCGGG GGATGAAATT CTGGAAGTTG AGACAGAGCA ACGGGCTCGG 
GAAGTGGTGG ACTGGAGGAA ATACGTGGAA GAGCAAGAGA AAATAATAGG GGATATGGAG 
GTAATTGAGG CCAAGCAGAA GGAACACCGA GATGCCTACA AGAAGAACCT GGAGACTTTT 
AGCAACTTGA ACTGGAGACA AAGAAAATCT GCCATGTACA AAGCCAACAA GCAGCTTATG 
GCCACGAGGC CTGGCGAGAA GTCAGAGAGC GAAACACCCA TGTTCCCCCT TATTATAAAA 
GGAGATGTTG ATGGGTCAGT AGAAGCCATT CTCAGTATTA TAGACACTTA TGATGCCGAA 
GACCAGTGTG AGCTTGATTT AGTGCACTTT GGAGTTGGGG ATATCTCTGA AAATGACATT 
GCGCTCGCTG AGACATTCAA AGGCTATGTA TATGGGTTCA ATGTGAATTC AAACAAAGCG 
GTTCAGCAAC TGGCGGCCAA AAAAGAGGTT ACAGTGAAAC TGCATAAAGT CATCTACCAT 
CTGGTGGAAG ATCTGAGAGA AGAACTCAGC AAACAGCTGC CTCCGGTAAT TGAAGAAAAT 
ATAAAAGGTG AGGCATCTGT GTTGGCGACA TTTGATGTCA CAGTTGGGAA GAAGAAGGTG 
CAGGTGGCAG GCTGCAGGGT ACAGAAAGGC ATTTTGGATA AGAAGGTGAA ATGTAAAGTG 
ATCCGAAACA GAAAGATGAT TTGGGAAGGA TATTTAACGT CACTTAAACA CCTCAAGGAC 
GATGTGCAGA CAGTAAAAAC CGGGGTGGAG TGCGGCCTGA GTCTGGATAA AGACATCGAT 
GTAAAAATTG GCGACGAAGT TCTGTGTGTT GAAGAAAAGG AAGTTCAGCA GAAAATCTCC 
TGGGATCCTG GATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mtif2 ( XM_002936912.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936958.1 Xenopus tropicalis mitochondrial translational initiation factor 2 (mtif2), transcript variant X2, mRNA.

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CloneID	OXa10837
Clone ID Related Accession (Same CDS sequence)	XM_002936912.4 , XM_004914740.3 , XM_002936912.5 , XM_004914740.4
Accession Version	XM_002936912.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	translation initiation factor IF-2, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002936912.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAGGA ATGTATTCAT GCAGTTGGAA CAGATGGTTC GACTTCATAA CACCTGCAGG 
AAATTGCATG CCCTGTGCCA CACCAATGCC AGGATAAAAT GGAGGCTTGT GCAGTGCCCT 
CCCTTAGCTG TATGTGTTGC CCACTTGTGC CCACCTTCCT GGCAAACAGA CACCTTCATC 
AGTGTGGCAC TGTCACAGTA TCGTTGTTTG TCCTCAAAAG AGGGCAAATC TTTTAAAAAG 
CACAAGTTGT CGCCGATCAA ACCTAAGCAA GAAAAGCAGG AAGTGGAGAT ATGGAACAAT 
ATGACCGTTG GTGATCTTGC AAGAGCCATG AATAAGGATA CAGAACACGT GTACGAAGCC 
CTTCTACATA CAGATCTTGA TTTGGAAGCT TTAGAAGAAA AGTCTGTCCT TAGCGAGAAG 
TGGATAAAGG AAGCAATTAA GAAATCTGGC ATGAAGTTTA AATATGCAAA GATAAAAGAG 
GAAAAAGTCA GAGAAAACAA GGACGCGGTG AAAAGAGTCC CTGCAGATCC AGCTGTACTG 
ATTCCCAGAC CCCCTGTAAT TACCATAATG GGGCATGTTG ACCATGGGAA AACGACCCTG 
CTTGACCAGC TAAGAAAAAC CCAAGTGGCT GCAATGGAAG CAGGGGGTAT AACCCAGCAC 
ATTGGTGCAT TTAACGTTCA GTTACCTTCA GGGGAGAGGA TAACCTTTTT GGATACTCCA 
GGACATGCAG CCTTTTCAGC AATCCGAGAA AGAGGAGCTA ATGTCACTGA TATTGTTATA 
CTGGTGGTGG CGGCAGAGGA TGGCGTTATG AAACAAACCG TTGAGTCCAT ACAACATGCC 
AAGAATGCAA AAGTCCCTAT TATCCTTGCC ATAAATAAAT GTGACAAACC AGAAGCCAGT 
CCCGAGAAAG TAAAGAAGGA GCTGCTGTCC CATGATATTG TGTGTGAGGA GTATGGAGGG 
GAAATCCAGG CTATTCACAT CTCTGCACTA AAGGGACACA ACTTGCAGGC ATTAACAGAA 
GCCACAGTCA CCTTAGCAGA AATGTTGGAG CTAAAAGCAG ACCCCACAGG TCTAGTGGAG 
GGAACTGTTG TAGAGTGTCG TACAGACAAA GGGAAAGGTC CAGTGACCAC AGCCATAGTG 
CAGAGAGGAA CTCTTAAAAA GGGTGCTGTG TTGGTAGCAG GTAAAACCTG GGCAAAGGTT 
CGCCTAATGT TTGATGAGAA CAACAAGGCT TTAAGCCAGG CGGGCCCTAG CACACCTGTG 
GAGATTGTTG GCTGGAAAGA TCTACCATCT GCGGGGGATG AAATTCTGGA AGTTGAGACA 
GAGCAACGGG CTCGGGAAGT GGTGGACTGG AGGAAATACG TGGAAGAGCA AGAGAAAATA 
ATAGGGGATA TGGAGGTAAT TGAGGCCAAG CAGAAGGAAC ACCGAGATGC CTACAAGAAG 
AACCTGGAGA CTTTTAGCAA CTTGAACTGG AGACAAAGAA AATCTGCCAT GTACAAAGCC 
AACAAGCAGC TTATGGCCAC GAGGCCTGGC GAGAAGTCAG AGAGCGAAAC ACCCATGTTC 
CCCCTTATTA TAAAAGGAGA TGTTGATGGG TCAGTAGAAG CCATTCTCAG TATTATAGAC 
ACTTATGATG CCGAAGACCA GTGTGAGCTT GATTTAGTGC ACTTTGGAGT TGGGGATATC 
TCTGAAAATG ACATTGCGCT CGCTGAGACA TTCAAAGGCT ATGTATATGG GTTCAATGTG 
AATTCAAACA AAGCGGTTCA GCAACTGGCG GCCAAAAAAG AGGTTACAGT GAAACTGCAT 
AAAGTCATCT ACCATCTGGT GGAAGATCTG AGAGAAGAAC TCAGCAAACA GCTGCCTCCG 
GTAATTGAAG AAAATATAAA AGGTGAGGCA TCTGTGTTGG CGACATTTGA TGTCACAGTT 
GGGAAGAAGA AGGTGCAGGT GGCAGGCTGC AGGGTACAGA AAGGCATTTT GGATAAGAAG 
GTGAAATGTA AAGTGATCCG AAACAGAAAG ATGATTTGGG AAGGATATTT AACGTCACTT 
AAACACCTCA AGGACGATGT GCAGACAGTA AAAACCGGGG TGGAGTGCGG CCTGAGTCTG 
GATAAAGACA TCGATGTAAA AATTGGCGAC GAAGTTCTGT GTGTTGAAGA AAAGGAAGTT 
CAGCAGAAAA TCTCCTGGGA TCCTGGATTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936958.1
CDS	229..2421
Translation	MNRNVFMQLEQMVRLHNTCRKLHALCHTNARIKWRLVQCPPLAVCVAHLCPPSWQTDTFISVALSQYRCLSSKEGKSFKKHKLSPIKPKQEKQEVEIWNNMTVGDLARAMNKDTEHVYEALLHTDLDLEALEEKSVLSEKWIKEAIKKSGMKFKYAKIKEEKVRENKDAVKRVPADPAVLIPRPPVITIMGHVDHGKTTLLDQLRKTQVAAMEAGGITQHIGAFNVQLPSGERITFLDTPGHAAFSAIRERGANVTDIVILVVAAEDGVMKQTVESIQHAKNAKVPIILAINKCDKPEASPEKVKKELLSHDIVCEEYGGEIQAIHISALKGHNLQALTEATVTLAEMLELKADPTGLVEGTVVECRTDKGKGPVTTAIVQRGTLKKGAVLVAGKTWAKVRLMFDENNKALSQAGPSTPVEIVGWKDLPSAGDEILEVETEQRAREVVDWRKYVEEQEKIIGDMEVIEAKQKEHRDAYKKNLETFSNLNWRQRKSAMYKANKQLMATRPGEKSESETPMFPLIIKGDVDGSVEAILSIIDTYDAEDQCELDLVHFGVGDISENDIALAETFKGYVYGFNVNSNKAVQQLAAKKEVTVKLHKVIYHLVEDLREELSKQLPPVIEENIKGEASVLATFDVTVGKKKVQVAGCRVQKGILDKKVKCKVIRNRKMIWEGYLTSLKHLKDDVQTVKTGVECGLSLDKDIDVKIGDEVLCVEEKEVQQKISWDPGF

Target ORF information:

RefSeq Version	XM_002936912.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitochondrial translational initiation factor 2 (mtif2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936912.5

ORF Insert Sequence:

ATGAACAGGA ATGTATTCAT GCAGTTGGAA CAGATGGTTC GACTTCATAA CACCTGCAGG 
AAATTGCATG CCCTGTGCCA CACCAATGCC AGGATAAAAT GGAGGCTTGT GCAGTGCCCT 
CCCTTAGCTG TATGTGTTGC CCACTTGTGC CCACCTTCCT GGCAAACAGA CACCTTCATC 
AGTGTGGCAC TGTCACAGTA TCGTTGTTTG TCCTCAAAAG AGGGCAAATC TTTTAAAAAG 
CACAAGTTGT CGCCGATCAA ACCTAAGCAA GAAAAGCAGG AAGTGGAGAT ATGGAACAAT 
ATGACCGTTG GTGATCTTGC AAGAGCCATG AATAAGGATA CAGAACACGT GTACGAAGCC 
CTTCTACATA CAGATCTTGA TTTGGAAGCT TTAGAAGAAA AGTCTGTCCT TAGCGAGAAG 
TGGATAAAGG AAGCAATTAA GAAATCTGGC ATGAAGTTTA AATATGCAAA GATAAAAGAG 
GAAAAAGTCA GAGAAAACAA GGACGCGGTG AAAAGAGTCC CTGCAGATCC AGCTGTACTG 
ATTCCCAGAC CCCCTGTAAT TACCATAATG GGGCATGTTG ACCATGGGAA AACGACCCTG 
CTTGACCAGC TAAGAAAAAC CCAAGTGGCT GCAATGGAAG CAGGGGGTAT AACCCAGCAC 
ATTGGTGCAT TTAACGTTCA GTTACCTTCA GGGGAGAGGA TAACCTTTTT GGATACTCCA 
GGACATGCAG CCTTTTCAGC AATCCGAGAA AGAGGAGCTA ATGTCACTGA TATTGTTATA 
CTGGTGGTGG CGGCAGAGGA TGGCGTTATG AAACAAACCG TTGAGTCCAT ACAACATGCC 
AAGAATGCAA AAGTCCCTAT TATCCTTGCC ATAAATAAAT GTGACAAACC AGAAGCCAGT 
CCCGAGAAAG TAAAGAAGGA GCTGCTGTCC CATGATATTG TGTGTGAGGA GTATGGAGGG 
GAAATCCAGG CTATTCACAT CTCTGCACTA AAGGGACACA ACTTGCAGGC ATTAACAGAA 
GCCACAGTCA CCTTAGCAGA AATGTTGGAG CTAAAAGCAG ACCCCACAGG TCTAGTGGAG 
GGAACTGTTG TAGAGTGTCG TACAGACAAA GGGAAAGGTC CAGTGACCAC AGCCATAGTG 
CAGAGAGGAA CTCTTAAAAA GGGTGCTGTG TTGGTAGCAG GTAAAACCTG GGCAAAGGTT 
CGCCTAATGT TTGATGAGAA CAACAAGGCT TTAAGCCAGG CGGGCCCTAG CACACCTGTG 
GAGATTGTTG GCTGGAAAGA TCTACCATCT GCGGGGGATG AAATTCTGGA AGTTGAGACA 
GAGCAACGGG CTCGGGAAGT GGTGGACTGG AGGAAATACG TGGAAGAGCA AGAGAAAATA 
ATAGGGGATA TGGAGGTAAT TGAGGCCAAG CAGAAGGAAC ACCGAGATGC CTACAAGAAG 
AACCTGGAGA CTTTTAGCAA CTTGAACTGG AGACAAAGAA AATCTGCCAT GTACAAAGCC 
AACAAGCAGC TTATGGCCAC GAGGCCTGGC GAGAAGTCAG AGAGCGAAAC ACCCATGTTC 
CCCCTTATTA TAAAAGGAGA TGTTGATGGG TCAGTAGAAG CCATTCTCAG TATTATAGAC 
ACTTATGATG CCGAAGACCA GTGTGAGCTT GATTTAGTGC ACTTTGGAGT TGGGGATATC 
TCTGAAAATG ACATTGCGCT CGCTGAGACA TTCAAAGGCT ATGTATATGG GTTCAATGTG 
AATTCAAACA AAGCGGTTCA GCAACTGGCG GCCAAAAAAG AGGTTACAGT GAAACTGCAT 
AAAGTCATCT ACCATCTGGT GGAAGATCTG AGAGAAGAAC TCAGCAAACA GCTGCCTCCG 
GTAATTGAAG AAAATATAAA AGGTGAGGCA TCTGTGTTGG CGACATTTGA TGTCACAGTT 
GGGAAGAAGA AGGTGCAGGT GGCAGGCTGC AGGGTACAGA AAGGCATTTT GGATAAGAAG 
GTGAAATGTA AAGTGATCCG AAACAGAAAG ATGATTTGGG AAGGATATTT AACGTCACTT 
AAACACCTCA AGGACGATGT GCAGACAGTA AAAACCGGGG TGGAGTGCGG CCTGAGTCTG 
GATAAAGACA TCGATGTAAA AATTGGCGAC GAAGTTCTGT GTGTTGAAGA AAAGGAAGTT 
CAGCAGAAAA TCTCCTGGGA TCCTGGATTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mtif2 ( XM_004914740.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914797.1 Xenopus tropicalis mitochondrial translational initiation factor 2 (mtif2), transcript variant X3, mRNA.

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CloneID	OXa10837
Clone ID Related Accession (Same CDS sequence)	XM_002936912.4 , XM_004914740.3 , XM_002936912.5 , XM_004914740.4
Accession Version	XM_004914740.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	translation initiation factor IF-2, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914740.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAGGA ATGTATTCAT GCAGTTGGAA CAGATGGTTC GACTTCATAA CACCTGCAGG 
AAATTGCATG CCCTGTGCCA CACCAATGCC AGGATAAAAT GGAGGCTTGT GCAGTGCCCT 
CCCTTAGCTG TATGTGTTGC CCACTTGTGC CCACCTTCCT GGCAAACAGA CACCTTCATC 
AGTGTGGCAC TGTCACAGTA TCGTTGTTTG TCCTCAAAAG AGGGCAAATC TTTTAAAAAG 
CACAAGTTGT CGCCGATCAA ACCTAAGCAA GAAAAGCAGG AAGTGGAGAT ATGGAACAAT 
ATGACCGTTG GTGATCTTGC AAGAGCCATG AATAAGGATA CAGAACACGT GTACGAAGCC 
CTTCTACATA CAGATCTTGA TTTGGAAGCT TTAGAAGAAA AGTCTGTCCT TAGCGAGAAG 
TGGATAAAGG AAGCAATTAA GAAATCTGGC ATGAAGTTTA AATATGCAAA GATAAAAGAG 
GAAAAAGTCA GAGAAAACAA GGACGCGGTG AAAAGAGTCC CTGCAGATCC AGCTGTACTG 
ATTCCCAGAC CCCCTGTAAT TACCATAATG GGGCATGTTG ACCATGGGAA AACGACCCTG 
CTTGACCAGC TAAGAAAAAC CCAAGTGGCT GCAATGGAAG CAGGGGGTAT AACCCAGCAC 
ATTGGTGCAT TTAACGTTCA GTTACCTTCA GGGGAGAGGA TAACCTTTTT GGATACTCCA 
GGACATGCAG CCTTTTCAGC AATCCGAGAA AGAGGAGCTA ATGTCACTGA TATTGTTATA 
CTGGTGGTGG CGGCAGAGGA TGGCGTTATG AAACAAACCG TTGAGTCCAT ACAACATGCC 
AAGAATGCAA AAGTCCCTAT TATCCTTGCC ATAAATAAAT GTGACAAACC AGAAGCCAGT 
CCCGAGAAAG TAAAGAAGGA GCTGCTGTCC CATGATATTG TGTGTGAGGA GTATGGAGGG 
GAAATCCAGG CTATTCACAT CTCTGCACTA AAGGGACACA ACTTGCAGGC ATTAACAGAA 
GCCACAGTCA CCTTAGCAGA AATGTTGGAG CTAAAAGCAG ACCCCACAGG TCTAGTGGAG 
GGAACTGTTG TAGAGTGTCG TACAGACAAA GGGAAAGGTC CAGTGACCAC AGCCATAGTG 
CAGAGAGGAA CTCTTAAAAA GGGTGCTGTG TTGGTAGCAG GTAAAACCTG GGCAAAGGTT 
CGCCTAATGT TTGATGAGAA CAACAAGGCT TTAAGCCAGG CGGGCCCTAG CACACCTGTG 
GAGATTGTTG GCTGGAAAGA TCTACCATCT GCGGGGGATG AAATTCTGGA AGTTGAGACA 
GAGCAACGGG CTCGGGAAGT GGTGGACTGG AGGAAATACG TGGAAGAGCA AGAGAAAATA 
ATAGGGGATA TGGAGGTAAT TGAGGCCAAG CAGAAGGAAC ACCGAGATGC CTACAAGAAG 
AACCTGGAGA CTTTTAGCAA CTTGAACTGG AGACAAAGAA AATCTGCCAT GTACAAAGCC 
AACAAGCAGC TTATGGCCAC GAGGCCTGGC GAGAAGTCAG AGAGCGAAAC ACCCATGTTC 
CCCCTTATTA TAAAAGGAGA TGTTGATGGG TCAGTAGAAG CCATTCTCAG TATTATAGAC 
ACTTATGATG CCGAAGACCA GTGTGAGCTT GATTTAGTGC ACTTTGGAGT TGGGGATATC 
TCTGAAAATG ACATTGCGCT CGCTGAGACA TTCAAAGGCT ATGTATATGG GTTCAATGTG 
AATTCAAACA AAGCGGTTCA GCAACTGGCG GCCAAAAAAG AGGTTACAGT GAAACTGCAT 
AAAGTCATCT ACCATCTGGT GGAAGATCTG AGAGAAGAAC TCAGCAAACA GCTGCCTCCG 
GTAATTGAAG AAAATATAAA AGGTGAGGCA TCTGTGTTGG CGACATTTGA TGTCACAGTT 
GGGAAGAAGA AGGTGCAGGT GGCAGGCTGC AGGGTACAGA AAGGCATTTT GGATAAGAAG 
GTGAAATGTA AAGTGATCCG AAACAGAAAG ATGATTTGGG AAGGATATTT AACGTCACTT 
AAACACCTCA AGGACGATGT GCAGACAGTA AAAACCGGGG TGGAGTGCGG CCTGAGTCTG 
GATAAAGACA TCGATGTAAA AATTGGCGAC GAAGTTCTGT GTGTTGAAGA AAAGGAAGTT 
CAGCAGAAAA TCTCCTGGGA TCCTGGATTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914797.1
CDS	91..2283
Translation	MNRNVFMQLEQMVRLHNTCRKLHALCHTNARIKWRLVQCPPLAVCVAHLCPPSWQTDTFISVALSQYRCLSSKEGKSFKKHKLSPIKPKQEKQEVEIWNNMTVGDLARAMNKDTEHVYEALLHTDLDLEALEEKSVLSEKWIKEAIKKSGMKFKYAKIKEEKVRENKDAVKRVPADPAVLIPRPPVITIMGHVDHGKTTLLDQLRKTQVAAMEAGGITQHIGAFNVQLPSGERITFLDTPGHAAFSAIRERGANVTDIVILVVAAEDGVMKQTVESIQHAKNAKVPIILAINKCDKPEASPEKVKKELLSHDIVCEEYGGEIQAIHISALKGHNLQALTEATVTLAEMLELKADPTGLVEGTVVECRTDKGKGPVTTAIVQRGTLKKGAVLVAGKTWAKVRLMFDENNKALSQAGPSTPVEIVGWKDLPSAGDEILEVETEQRAREVVDWRKYVEEQEKIIGDMEVIEAKQKEHRDAYKKNLETFSNLNWRQRKSAMYKANKQLMATRPGEKSESETPMFPLIIKGDVDGSVEAILSIIDTYDAEDQCELDLVHFGVGDISENDIALAETFKGYVYGFNVNSNKAVQQLAAKKEVTVKLHKVIYHLVEDLREELSKQLPPVIEENIKGEASVLATFDVTVGKKKVQVAGCRVQKGILDKKVKCKVIRNRKMIWEGYLTSLKHLKDDVQTVKTGVECGLSLDKDIDVKIGDEVLCVEEKEVQQKISWDPGF

Target ORF information:

RefSeq Version	XM_004914740.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mitochondrial translational initiation factor 2 (mtif2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914740.4

ORF Insert Sequence:

ATGAACAGGA ATGTATTCAT GCAGTTGGAA CAGATGGTTC GACTTCATAA CACCTGCAGG 
AAATTGCATG CCCTGTGCCA CACCAATGCC AGGATAAAAT GGAGGCTTGT GCAGTGCCCT 
CCCTTAGCTG TATGTGTTGC CCACTTGTGC CCACCTTCCT GGCAAACAGA CACCTTCATC 
AGTGTGGCAC TGTCACAGTA TCGTTGTTTG TCCTCAAAAG AGGGCAAATC TTTTAAAAAG 
CACAAGTTGT CGCCGATCAA ACCTAAGCAA GAAAAGCAGG AAGTGGAGAT ATGGAACAAT 
ATGACCGTTG GTGATCTTGC AAGAGCCATG AATAAGGATA CAGAACACGT GTACGAAGCC 
CTTCTACATA CAGATCTTGA TTTGGAAGCT TTAGAAGAAA AGTCTGTCCT TAGCGAGAAG 
TGGATAAAGG AAGCAATTAA GAAATCTGGC ATGAAGTTTA AATATGCAAA GATAAAAGAG 
GAAAAAGTCA GAGAAAACAA GGACGCGGTG AAAAGAGTCC CTGCAGATCC AGCTGTACTG 
ATTCCCAGAC CCCCTGTAAT TACCATAATG GGGCATGTTG ACCATGGGAA AACGACCCTG 
CTTGACCAGC TAAGAAAAAC CCAAGTGGCT GCAATGGAAG CAGGGGGTAT AACCCAGCAC 
ATTGGTGCAT TTAACGTTCA GTTACCTTCA GGGGAGAGGA TAACCTTTTT GGATACTCCA 
GGACATGCAG CCTTTTCAGC AATCCGAGAA AGAGGAGCTA ATGTCACTGA TATTGTTATA 
CTGGTGGTGG CGGCAGAGGA TGGCGTTATG AAACAAACCG TTGAGTCCAT ACAACATGCC 
AAGAATGCAA AAGTCCCTAT TATCCTTGCC ATAAATAAAT GTGACAAACC AGAAGCCAGT 
CCCGAGAAAG TAAAGAAGGA GCTGCTGTCC CATGATATTG TGTGTGAGGA GTATGGAGGG 
GAAATCCAGG CTATTCACAT CTCTGCACTA AAGGGACACA ACTTGCAGGC ATTAACAGAA 
GCCACAGTCA CCTTAGCAGA AATGTTGGAG CTAAAAGCAG ACCCCACAGG TCTAGTGGAG 
GGAACTGTTG TAGAGTGTCG TACAGACAAA GGGAAAGGTC CAGTGACCAC AGCCATAGTG 
CAGAGAGGAA CTCTTAAAAA GGGTGCTGTG TTGGTAGCAG GTAAAACCTG GGCAAAGGTT 
CGCCTAATGT TTGATGAGAA CAACAAGGCT TTAAGCCAGG CGGGCCCTAG CACACCTGTG 
GAGATTGTTG GCTGGAAAGA TCTACCATCT GCGGGGGATG AAATTCTGGA AGTTGAGACA 
GAGCAACGGG CTCGGGAAGT GGTGGACTGG AGGAAATACG TGGAAGAGCA AGAGAAAATA 
ATAGGGGATA TGGAGGTAAT TGAGGCCAAG CAGAAGGAAC ACCGAGATGC CTACAAGAAG 
AACCTGGAGA CTTTTAGCAA CTTGAACTGG AGACAAAGAA AATCTGCCAT GTACAAAGCC 
AACAAGCAGC TTATGGCCAC GAGGCCTGGC GAGAAGTCAG AGAGCGAAAC ACCCATGTTC 
CCCCTTATTA TAAAAGGAGA TGTTGATGGG TCAGTAGAAG CCATTCTCAG TATTATAGAC 
ACTTATGATG CCGAAGACCA GTGTGAGCTT GATTTAGTGC ACTTTGGAGT TGGGGATATC 
TCTGAAAATG ACATTGCGCT CGCTGAGACA TTCAAAGGCT ATGTATATGG GTTCAATGTG 
AATTCAAACA AAGCGGTTCA GCAACTGGCG GCCAAAAAAG AGGTTACAGT GAAACTGCAT 
AAAGTCATCT ACCATCTGGT GGAAGATCTG AGAGAAGAAC TCAGCAAACA GCTGCCTCCG 
GTAATTGAAG AAAATATAAA AGGTGAGGCA TCTGTGTTGG CGACATTTGA TGTCACAGTT 
GGGAAGAAGA AGGTGCAGGT GGCAGGCTGC AGGGTACAGA AAGGCATTTT GGATAAGAAG 
GTGAAATGTA AAGTGATCCG AAACAGAAAG ATGATTTGGG AAGGATATTT AACGTCACTT 
AAACACCTCA AGGACGATGT GCAGACAGTA AAAACCGGGG TGGAGTGCGG CCTGAGTCTG 
GATAAAGACA TCGATGTAAA AATTGGCGAC GAAGTTCTGT GTGTTGAAGA AAAGGAAGTT 
CAGCAGAAAA TCTCCTGGGA TCCTGGATTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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mtif2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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