pcsk1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	pcsk1
Entrez Gene ID	100496933
Full Name	proprotein convertase subtilisin/kexin type 1
General protein information	Preferred Names proprotein convertase subtilisin/kexin type 1 Names neuroendocrine convertase 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004910458.3	XP_004910515.3	neuroendocrine convertase 1 isoform X1
XM_018090795.1	XP_017946284.1	neuroendocrine convertase 1 isoform X2
XM_018090795.2	XP_017946284.1	neuroendocrine convertase 1 isoform X2
XM_004910458.2	XP_004910515.2	neuroendocrine convertase 1 isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	pcsk1	XP_004910515.1
Homo sapiens (human)	PCSK1	NP_000430.3
Danio rerio (zebrafish)	pcsk1	NP_001131134.1
Bos taurus (cattle)	PCSK1	NP_776837.1
Gallus gallus (chicken)	PCSK1	XP_003643108.1
Caenorhabditis elegans (roundworm)	bli-4	NP_001021543.1
Canis lupus familiaris (dog)	PCSK1	XP_848637.1
Pan troglodytes (chimpanzee)	PCSK1	XP_001134900.1
	PCSK1	XP_002804496.1
Mus musculus (house mouse)	Pcsk1	NP_038656.1

Gene Ontology

The following pcsk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pcsk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa36726	XM_004910458.2 Latest version!	Xenopus tropicalis proprotein convertase subtilisin/kexin type 1 (pcsk1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa42228	XM_018090795.2 Latest version!	Xenopus tropicalis proprotein convertase subtilisin/kexin type 1 (pcsk1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa49067	XM_004910458.3 Latest version!	Xenopus tropicalis proprotein convertase subtilisin/kexin type 1 (pcsk1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OXa42228	XM_018090795.1	Xenopus tropicalis proprotein convertase subtilisin/kexin type 1 (pcsk1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pcsk1 ( XM_004910458.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910515.2 Xenopus tropicalis proprotein convertase subtilisin/kexin type 1 (pcsk1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa36726
Clone ID Related Accession (Same CDS sequence)	XM_004910458.2
Accession Version	XM_004910458.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neuroendocrine convertase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_004910458.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCATAT GGGAGCGCAC TGTTTACTCC AGTGATGCCG GCAGCCTAGG AATAGCTTCC 
CTAGTATTTG CAGTGCAGCA GCTGATTTGC GGGCTGCTGA GCATGATTTG CCTTTGTGTG 
TGGGCGTCTC CAGCTGTAAT GAGTGAGCTC AGTGTAGTGG AGCTGCGAGC ACCCAGCACT 
TGCTATTTTA GCCTCTCACC TCCCTCGGGA ATACCGTATA GATTGAAGAC GCCTGGAAGT 
GTGTTTGACG TCGTTGTGAC GTGTAAAAGA CACATTTCCA ATGAAGCTGC TAAAGGACCG 
AGCATTTTTC TAGCCTCGGA GAGAGAGAGA AACCTGCTGC TCTTATCTGT TCGCATAGCA 
ACCAGGACCA AGCACCCCGA TTTGAAATTC TCATTGGACT CTTTTAGGGG TTTTGGCTCA 
GACACTGTGA GAATGGAAGG ACGATACTGG TACCCCATGT GCATTGCTTC AGTCGCTGTC 
TTGTCCTTGT TGTGTCAGGT GCTAGCTGCT GGGGTGGATA AACGGTATAT GAATGAATGG 
GCAGCAGAGA TCCCCGGGGG GCCCGAGGAA GCCCAGTTAC TTGCATTGGA GTTGGATTAT 
GATTACCTGG GACAGATTGG ATCCTTAGAA AATCACTACC TGTTCAGACA CAAAGCTCAT 
CCAAGAAGAT CACGCAGAAG TGCCCCTCAT CTTACAAAGA GACTAGCTGA TGATGGGCGG 
GTGTCGTGGG CGGAACAACA GTACAGTAAA GAGAGAAATA AGCGGTCAAC CTTCAGACAA 
GACACCAAAA ACCTGTTTAA TGATCCCCTG TGGAATCGAC AATGGTACTT GCAAGACACA 
AGATCGAATC CCTCATTGCC TAAGTTGGAT TTACATCTCC TTCCTGTATG GAAGAAAGGA 
ATAACAGGCA AAGGTGTTGT TATCACAGTG CTGGATGATG GTTTGGAATG GAACCACACT 
GATATCTATG CCAATTACGA CCCAGAAGCA AGCTATGATT TCAATGACAA TGACAATGAC 
CCCTTTCCCA GATATGATAT TACAAATGAA AACAGACACG GGACAAGATG TGCTGGGGAA 
ATTGCCATGG TTGCCAATAA CAATAAATGT GGAGTCGGAG TTGCATATAA TGCAAAGGTT 
GGGGGCATAC GGATGCTGGA TGGTGTTGTG ACAGATGCCA AAGAAGCAAG TTCCATTGGC 
TTCAATCCAC AACATGTGCA TATATACAGT GCCAGCTGGG GACCTAATGA TGATGGCAAA 
ACCGTAGAAG GACCGGGTAG ACTGGCACAG AAAGCTTTTG AGTATGGCAT CAAACAGGGG 
CGAAATGGAA AAGGTTCCAT CTTTGTTTGG GCATCTGGAA ATGGTGGGCG ACAGGGTGAT 
AACTGTGACT GTGATGGATA CACAGACAGC ATTTACACCA TCTCTATCAG CAGTGCTTCC 
CAGCAAGGTT TGTCTCCCTG GTATGCTGAG AAATGCTCTT CCACCCTGGC CACAGCATAC 
AGCAGTGGGG ACTACACAGA TCAGAGAATT ATAAGCGCCG ACTTACACAA TGAGTGCACA 
GAGACGCACA CCGGCACCTC TGCTTCAGCT CCGCTGGCTG CCGGGATCTT TGCTCTCGCA 
CTGGAATTTA ACCCTGACCT TACCTGGAGG GATATGCAGC ACCTGGTGGT TTGGACATCA 
GAGTACGACC CGTTGGCTAA CAATGCTGGC TGGAAGAAAA ATGGCGCTGG GCTAATGGTT 
AATAGCAGAT TTGGATTTGG GCTGCTCAAT GCTAAGGCAC TTGTGGATTT AGCTGACCCT 
AAAACATGGA AAGGAGTTGC AGAAAAGAAG GAGTGTATAG TAGAAGATAA TGAGTTTTCA 
CCAAGGTTTC TAAGATCAGA TGGGGAAGTA ACCATTCAAA TTCCTACAAA AGCCTGTGAA 
GGCCAAGATA ACCATATCAA GTCTCTGGAG CACTTGCAGC TTGAAGCCAC AATTGAATAC 
ACCCGTAGGG GGGACCTTCA CATTACACTC ACCTCTCCAT TAGGGACTAA TACAGTTCTT 
CTGACTGAAA GAGAGAGAGA TACTTCTCCA TATGGTTTCA AAAGCTGGGA CTTTATGTCT 
GTGCATACGT GGGGAGAAGA TCCAGCGGGA ACCTGGACGC TAAAAATCTC TGATGTGTCT 
GGAAGAAAAA AAAATGAAGG AAGAATAGTC AGCTGGAAAT TGATTCTTCA TGGAACATCG 
ACCCAACCGG AGCATATGAA ACATCCAAGA GTTTATACTT CCTATAATGT TGTTCAAAAT 
GATCGAAGAG GCGCTGAGAA GATGGATGAC ATTGAAAAGG AACCAATCAG TGAAAGTTTC 
ACAACTGAGC GGCCAGAAGT CACTAAAAAC ACTCCAGACA GTCAAGAAGC TGCTGAAAGG 
GCAAAGTCGC TAGCAATAGC CCACCTTTTG AAGAATGCCT TCGACAGAGA CGGAGGTTCA 
TTTCCTCTAG AAGACAAAGA TATCCCTAAA CATAGTTATT TGTATGCTCT GGCCACCATT 
AGCAAGCAGG GAGAAGGAAG GGACAGAGAG AACGATTTGT ACAATGAGTA CATTGACAGG 
TTCTACCATT CCAGACCTTA CAAACACAGA GATGATAGAC TATTGCAAGC TTTGTTCAAC 
ATTTTAAAGA AGAAACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910515.2
CDS	2..2662
Translation	MFIWERTVYSSDAGSLGIASLVFAVQQLICGLLSMICLCVWASPAVMSELSVVELRAPSTCYFSLSPPSGIPYRLKTPGSVFDVVVTCKRHISNEAAKGPSIFLASERERNLLLLSVRIATRTKHPDLKFSLDSFRGFGSDTVRMEGRYWYPMCIASVAVLSLLCQVLAAGVDKRYMNEWAAEIPGGPEEAQLLALELDYDYLGQIGSLENHYLFRHKAHPRRSRRSAPHLTKRLADDGRVSWAEQQYSKERNKRSTFRQDTKNLFNDPLWNRQWYLQDTRSNPSLPKLDLHLLPVWKKGITGKGVVITVLDDGLEWNHTDIYANYDPEASYDFNDNDNDPFPRYDITNENRHGTRCAGEIAMVANNNKCGVGVAYNAKVGGIRMLDGVVTDAKEASSIGFNPQHVHIYSASWGPNDDGKTVEGPGRLAQKAFEYGIKQGRNGKGSIFVWASGNGGRQGDNCDCDGYTDSIYTISISSASQQGLSPWYAEKCSSTLATAYSSGDYTDQRIISADLHNECTETHTGTSASAPLAAGIFALALEFNPDLTWRDMQHLVVWTSEYDPLANNAGWKKNGAGLMVNSRFGFGLLNAKALVDLADPKTWKGVAEKKECIVEDNEFSPRFLRSDGEVTIQIPTKACEGQDNHIKSLEHLQLEATIEYTRRGDLHITLTSPLGTNTVLLTERERDTSPYGFKSWDFMSVHTWGEDPAGTWTLKISDVSGRKKNEGRIVSWKLILHGTSTQPEHMKHPRVYTSYNVVQNDRRGAEKMDDIEKEPISESFTTERPEVTKNTPDSQEAAERAKSLAIAHLLKNAFDRDGGSFPLEDKDIPKHSYLYALATISKQGEGRDRENDLYNEYIDRFYHSRPYKHRDDRLLQALFNILKKKP

Target ORF information:

RefSeq Version	XM_004910458.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis proprotein convertase subtilisin/kexin type 1 (pcsk1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910458.2

ORF Insert Sequence:

ATGTTCATAT GGGAGCGCAC TGTTTACTCC AGTGATGCCG GCAGCCTAGG AATAGCTTCC 
CTAGTATTTG CAGTGCAGCA GCTGATTTGC GGGCTGCTGA GCATGATTTG CCTTTGTGTG 
TGGGCGTCTC CAGCTGTAAT GAGTGAGCTC AGTGTAGTGG AGCTGCGAGC ACCCAGCACT 
TGCTATTTTA GCCTCTCACC TCCCTCGGGA ATACCGTATA GATTGAAGAC GCCTGGAAGT 
GTGTTTGACG TCGTTGTGAC GTGTAAAAGA CACATTTCCA ATGAAGCTGC TAAAGGACCG 
AGCATTTTTC TAGCCTCGGA GAGAGAGAGA AACCTGCTGC TCTTATCTGT TCGCATAGCA 
ACCAGGACCA AGCACCCCGA TTTGAAATTC TCATTGGACT CTTTTAGGGG TTTTGGCTCA 
GACACTGTGA GAATGGAAGG ACGATACTGG TACCCCATGT GCATTGCTTC AGTCGCTGTC 
TTGTCCTTGT TGTGTCAGGT GCTAGCTGCT GGGGTGGATA AACGGTATAT GAATGAATGG 
GCAGCAGAGA TCCCCGGGGG GCCCGAGGAA GCCCAGTTAC TTGCATTGGA GTTGGATTAT 
GATTACCTGG GACAGATTGG ATCCTTAGAA AATCACTACC TGTTCAGACA CAAAGCTCAT 
CCAAGAAGAT CACGCAGAAG TGCCCCTCAT CTTACAAAGA GACTAGCTGA TGATGGGCGG 
GTGTCGTGGG CGGAACAACA GTACAGTAAA GAGAGAAATA AGCGGTCAAC CTTCAGACAA 
GACACCAAAA ACCTGTTTAA TGATCCCCTG TGGAATCGAC AATGGTACTT GCAAGACACA 
AGATCGAATC CCTCATTGCC TAAGTTGGAT TTACATCTCC TTCCTGTATG GAAGAAAGGA 
ATAACAGGCA AAGGTGTTGT TATCACAGTG CTGGATGATG GTTTGGAATG GAACCACACT 
GATATCTATG CCAATTACGA CCCAGAAGCA AGCTATGATT TCAATGACAA TGACAATGAC 
CCCTTTCCCA GATATGATAT TACAAATGAA AACAGACACG GGACAAGATG TGCTGGGGAA 
ATTGCCATGG TTGCCAATAA CAATAAATGT GGAGTCGGAG TTGCATATAA TGCAAAGGTT 
GGGGGCATAC GGATGCTGGA TGGTGTTGTG ACAGATGCCA AAGAAGCAAG TTCCATTGGC 
TTCAATCCAC AACATGTGCA TATATACAGT GCCAGCTGGG GACCTAATGA TGATGGCAAA 
ACCGTAGAAG GACCGGGTAG ACTGGCACAG AAAGCTTTTG AGTATGGCAT CAAACAGGGG 
CGAAATGGAA AAGGTTCCAT CTTTGTTTGG GCATCTGGAA ATGGTGGGCG ACAGGGTGAT 
AACTGTGACT GTGATGGATA CACAGACAGC ATTTACACCA TCTCTATCAG CAGTGCTTCC 
CAGCAAGGTT TGTCTCCCTG GTATGCTGAG AAATGCTCTT CCACCCTGGC CACAGCATAC 
AGCAGTGGGG ACTACACAGA TCAGAGAATT ATAAGCGCCG ACTTACACAA TGAGTGCACA 
GAGACGCACA CCGGCACCTC TGCTTCAGCT CCGCTGGCTG CCGGGATCTT TGCTCTCGCA 
CTGGAATTTA ACCCTGACCT TACCTGGAGG GATATGCAGC ACCTGGTGGT TTGGACATCA 
GAGTACGACC CGTTGGCTAA CAATGCTGGC TGGAAGAAAA ATGGCGCTGG GCTAATGGTT 
AATAGCAGAT TTGGATTTGG GCTGCTCAAT GCTAAGGCAC TTGTGGATTT AGCTGACCCT 
AAAACATGGA AAGGAGTTGC AGAAAAGAAG GAGTGTATAG TAGAAGATAA TGAGTTTTCA 
CCAAGGTTTC TAAGATCAGA TGGGGAAGTA ACCATTCAAA TTCCTACAAA AGCCTGTGAA 
GGCCAAGATA ACCATATCAA GTCTCTGGAG CACTTGCAGC TTGAAGCCAC AATTGAATAC 
ACCCGTAGGG GGGACCTTCA CATTACACTC ACCTCTCCAT TAGGGACTAA TACAGTTCTT 
CTGACTGAAA GAGAGAGAGA TACTTCTCCA TATGGTTTCA AAAGCTGGGA CTTTATGTCT 
GTGCATACGT GGGGAGAAGA TCCAGCGGGA ACCTGGACGC TAAAAATCTC TGATGTGTCT 
GGAAGAAAAA AAAATGAAGG AAGAATAGTC AGCTGGAAAT TGATTCTTCA TGGAACATCG 
ACCCAACCGG AGCATATGAA ACATCCAAGA GTTTATACTT CCTATAATGT TGTTCAAAAT 
GATCGAAGAG GCGCTGAGAA GATGGATGAC ATTGAAAAGG AACCAATCAG TGAAAGTTTC 
ACAACTGAGC GGCCAGAAGT CACTAAAAAC ACTCCAGACA GTCAAGAAGC TGCTGAAAGG 
GCAAAGTCGC TAGCAATAGC CCACCTTTTG AAGAATGCCT TCGACAGAGA CGGAGGTTCA 
TTTCCTCTAG AAGACAAAGA TATCCCTAAA CATAGTTATT TGTATGCTCT GGCCACCATT 
AGCAAGCAGG GAGAAGGAAG GGACAGAGAG AACGATTTGT ACAATGAGTA CATTGACAGG 
TTCTACCATT CCAGACCTTA CAAACACAGA GATGATAGAC TATTGCAAGC TTTGTTCAAC 
ATTTTAAAGA AGAAACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pcsk1 ( XM_018090795.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946284.1 Xenopus tropicalis proprotein convertase subtilisin/kexin type 1 (pcsk1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa42228
Clone ID Related Accession (Same CDS sequence)	XM_018090795.1 , XM_018090795.2
Accession Version	XM_018090795.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neuroendocrine convertase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGAC GATACTGGTA CCCCGTGTGC ATTGCTTCAG TCGCTGTCTT GTCCTTGTTG 
TGTCAGGTGC TAGCGGTTGG GGTGGATAAA CGGTATGTGA ATGAATGGGC AGCAGAGATC 
CCCGGGGGGC CCGAGGAAGC CCAGTTACTT GCATTGGAGT TGGATTATGA TTACCTGGGA 
CAGATTGGAT CCTTAGAAAA TCACTACCTG TTCAGACACA AAGCTCATCC AAGAAGATCA 
CGCAGAAGTG CCCCTCATCT TACAAAGAGA CTAGCTGATG ATGGGCGGGT GTCGTGGGCG 
GAACAACAGT ACAGTAAAGA GAGAAATAAG CGGTCAACCT TCAGACAAGA CACCAAAAAC 
CTGTTTAATG ATCCCCTGTG GAATCGACAA TGGTACTTGC AAGACACAAG ATCGAATCCC 
TCATTGCCTA AGTTGGATTT ACATCTCCTT CCTGTATGGA AGAAAGGAAT AACAGGCAAA 
GGTGTTGTTA TCACAGTGCT GGATGATGGT TTGGAATGGA ACCACACTGA TATCTATGCC 
AATTACGACC CAGAAGCAAG CTATGATTTC AATGACAATG ACAATGACCC CTTTCCCAGA 
TATGATATTA CAAATGAAAA CAGACACGGG ACAAGATGTG CTGGGGAAAT TGCCATGGTT 
GCCAATAACA ATAAATGTGG AGTCGGAGTT GCATATAATG CAAAGGTTGG GGGCATACGG 
ATGCTGGATG GTGTTGTGAC AGATGCCAAA GAAGCAAGTT CCATTGGCTT CAATCCACAA 
CATGTGCATA TATACAGTGC CAGCTGGGGA CCTAATGATG ATGGCAAAAC CGTAGAAGGA 
CCGGGTAGAC TGGCACAGAA AGCTTTTGAG TATGGCATCA AACAGGGGCG AAATGGAAAA 
GGTTCCATCT TTGTTTGGGC ATCTGGAAAT GGTGGGCGAC AGGGTGATAA CTGTGACTGT 
GATGGATACA CAGACAGCAT TTACACCATC TCTATCAGCA GTGCTTCCCA GCAAGGTTTG 
TCTCCCTGGT ATGCTGAGAA ATGCTCTTCC ACCCTGGCCA CAGCATACAG CAGTGGGGAC 
TACACAGATC AGAGAATTAT AAGCGCCGAC TTACACAATG AGTGCACAGA GACGCACACC 
GGCACCTCTG CTTCAGCTCC GCTGGCTGCC GGGATCTTTG CTCTCGCACT GGAATTTAAC 
CCTGACCTTA CCTGGAGGGA TATGCAGCAC CTGGTGGTTT GGACATCAGA GTACGACCCG 
TTGGCTAACA ATGCTGGCTG GAAGAAAAAT GGCGCTGGGC TAATGGTTAA TAGCAGATTT 
GGATTTGGGC TGCTCAATGC TAAGGCACTT GTGGATTTAG CTGACCCTAA AACATGGAAA 
GGAGTTGCAG AAAAGAAGGA GTGTATAGTA GAAGATAATG AGTTTTCACC AAGGTTTCTA 
AGATCAGATG GGGAAGTAAC CATTCAAATT CCTACAAAAG CCTGTGAAGG CCAAGATAAC 
CATATCAAGT CTCTGGAGCA CTTGCAGCTT GAAGCCACAA TTGAATACAC CCGTAGGGGG 
GACCTTCACA TTACACTCAC CTCTCCATTA GGGACTAATA CAGTTCTTCT GACTGAAAGA 
GAGAGAGATA CTTCTCCATA TGGTTTCAAA AGCTGGGACT TTATGTCTGT GCATACGTGG 
GGAGAAGATC CAGCGGGAAC CTGGACGCTA AAAATCTCTG ATGTGTCTGG AAGAAAAAAA 
AATGAAGGAA GAATAGTCAG CTGGAAATTG ATTCTTCATG GAACATCGAC CCAACCGGAG 
CATATGAAAC ATCCAAGAGT TTATACTTCC TATAATGTTG TTCAAAATGA TCGAAGAGGC 
GCTGAGAAGA TGGATGACAT TGAAAAGGAA CCAATCAGTG AAAGTTTCAC AACTGAGCGG 
CCAGAAGTCA CTAAAAACAC TCCAGACAGT CAAGAAGCTG CTGAAAGGGC AAAGTCGCTA 
GCAATAGCCC ACCTTTTGAA GAATGCCTTC GACAGAGACG GAGGTTCATT TCCTCTAGAA 
GACAAAGATA TCCCTAAACA TAGTTATTTG TATGCTCTGG CCACCATTAG CAAGCAGGGA 
GAAGGAAGGG ACAGAGAGAA CGATTTGTAC AATGAGTACA TTGACAGGTT CTACCATTCC 
AGACCTTACA AACACAGAGA TGATAGACTA TTGCAAGCTT TGTTCAACAT TTTAAAGAAG 
AAACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946284.1
CDS	433..2661
Translation	MEGRYWYPVCIASVAVLSLLCQVLAVGVDKRYVNEWAAEIPGGPEEAQLLALELDYDYLGQIGSLENHYLFRHKAHPRRSRRSAPHLTKRLADDGRVSWAEQQYSKERNKRSTFRQDTKNLFNDPLWNRQWYLQDTRSNPSLPKLDLHLLPVWKKGITGKGVVITVLDDGLEWNHTDIYANYDPEASYDFNDNDNDPFPRYDITNENRHGTRCAGEIAMVANNNKCGVGVAYNAKVGGIRMLDGVVTDAKEASSIGFNPQHVHIYSASWGPNDDGKTVEGPGRLAQKAFEYGIKQGRNGKGSIFVWASGNGGRQGDNCDCDGYTDSIYTISISSASQQGLSPWYAEKCSSTLATAYSSGDYTDQRIISADLHNECTETHTGTSASAPLAAGIFALALEFNPDLTWRDMQHLVVWTSEYDPLANNAGWKKNGAGLMVNSRFGFGLLNAKALVDLADPKTWKGVAEKKECIVEDNEFSPRFLRSDGEVTIQIPTKACEGQDNHIKSLEHLQLEATIEYTRRGDLHITLTSPLGTNTVLLTERERDTSPYGFKSWDFMSVHTWGEDPAGTWTLKISDVSGRKKNEGRIVSWKLILHGTSTQPEHMKHPRVYTSYNVVQNDRRGAEKMDDIEKEPISESFTTERPEVTKNTPDSQEAAERAKSLAIAHLLKNAFDRDGGSFPLEDKDIPKHSYLYALATISKQGEGRDRENDLYNEYIDRFYHSRPYKHRDDRLLQALFNILKKKP

Target ORF information:

RefSeq Version	XM_018090795.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis proprotein convertase subtilisin/kexin type 1 (pcsk1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090795.1

ORF Insert Sequence:

ATGGAAGGAC GATACTGGTA CCCCGTGTGC ATTGCTTCAG TCGCTGTCTT GTCCTTGTTG 
TGTCAGGTGC TAGCGGTTGG GGTGGATAAA CGGTATGTGA ATGAATGGGC AGCAGAGATC 
CCCGGGGGGC CCGAGGAAGC CCAGTTACTT GCATTGGAGT TGGATTATGA TTACCTGGGA 
CAGATTGGAT CCTTAGAAAA TCACTACCTG TTCAGACACA AAGCTCATCC AAGAAGATCA 
CGCAGAAGTG CCCCTCATCT TACAAAGAGA CTAGCTGATG ATGGGCGGGT GTCGTGGGCG 
GAACAACAGT ACAGTAAAGA GAGAAATAAG CGGTCAACCT TCAGACAAGA CACCAAAAAC 
CTGTTTAATG ATCCCCTGTG GAATCGACAA TGGTACTTGC AAGACACAAG ATCGAATCCC 
TCATTGCCTA AGTTGGATTT ACATCTCCTT CCTGTATGGA AGAAAGGAAT AACAGGCAAA 
GGTGTTGTTA TCACAGTGCT GGATGATGGT TTGGAATGGA ACCACACTGA TATCTATGCC 
AATTACGACC CAGAAGCAAG CTATGATTTC AATGACAATG ACAATGACCC CTTTCCCAGA 
TATGATATTA CAAATGAAAA CAGACACGGG ACAAGATGTG CTGGGGAAAT TGCCATGGTT 
GCCAATAACA ATAAATGTGG AGTCGGAGTT GCATATAATG CAAAGGTTGG GGGCATACGG 
ATGCTGGATG GTGTTGTGAC AGATGCCAAA GAAGCAAGTT CCATTGGCTT CAATCCACAA 
CATGTGCATA TATACAGTGC CAGCTGGGGA CCTAATGATG ATGGCAAAAC CGTAGAAGGA 
CCGGGTAGAC TGGCACAGAA AGCTTTTGAG TATGGCATCA AACAGGGGCG AAATGGAAAA 
GGTTCCATCT TTGTTTGGGC ATCTGGAAAT GGTGGGCGAC AGGGTGATAA CTGTGACTGT 
GATGGATACA CAGACAGCAT TTACACCATC TCTATCAGCA GTGCTTCCCA GCAAGGTTTG 
TCTCCCTGGT ATGCTGAGAA ATGCTCTTCC ACCCTGGCCA CAGCATACAG CAGTGGGGAC 
TACACAGATC AGAGAATTAT AAGCGCCGAC TTACACAATG AGTGCACAGA GACGCACACC 
GGCACCTCTG CTTCAGCTCC GCTGGCTGCC GGGATCTTTG CTCTCGCACT GGAATTTAAC 
CCTGACCTTA CCTGGAGGGA TATGCAGCAC CTGGTGGTTT GGACATCAGA GTACGACCCG 
TTGGCTAACA ATGCTGGCTG GAAGAAAAAT GGCGCTGGGC TAATGGTTAA TAGCAGATTT 
GGATTTGGGC TGCTCAATGC TAAGGCACTT GTGGATTTAG CTGACCCTAA AACATGGAAA 
GGAGTTGCAG AAAAGAAGGA GTGTATAGTA GAAGATAATG AGTTTTCACC AAGGTTTCTA 
AGATCAGATG GGGAAGTAAC CATTCAAATT CCTACAAAAG CCTGTGAAGG CCAAGATAAC 
CATATCAAGT CTCTGGAGCA CTTGCAGCTT GAAGCCACAA TTGAATACAC CCGTAGGGGG 
GACCTTCACA TTACACTCAC CTCTCCATTA GGGACTAATA CAGTTCTTCT GACTGAAAGA 
GAGAGAGATA CTTCTCCATA TGGTTTCAAA AGCTGGGACT TTATGTCTGT GCATACGTGG 
GGAGAAGATC CAGCGGGAAC CTGGACGCTA AAAATCTCTG ATGTGTCTGG AAGAAAAAAA 
AATGAAGGAA GAATAGTCAG CTGGAAATTG ATTCTTCATG GAACATCGAC CCAACCGGAG 
CATATGAAAC ATCCAAGAGT TTATACTTCC TATAATGTTG TTCAAAATGA TCGAAGAGGC 
GCTGAGAAGA TGGATGACAT TGAAAAGGAA CCAATCAGTG AAAGTTTCAC AACTGAGCGG 
CCAGAAGTCA CTAAAAACAC TCCAGACAGT CAAGAAGCTG CTGAAAGGGC AAAGTCGCTA 
GCAATAGCCC ACCTTTTGAA GAATGCCTTC GACAGAGACG GAGGTTCATT TCCTCTAGAA 
GACAAAGATA TCCCTAAACA TAGTTATTTG TATGCTCTGG CCACCATTAG CAAGCAGGGA 
GAAGGAAGGG ACAGAGAGAA CGATTTGTAC AATGAGTACA TTGACAGGTT CTACCATTCC 
AGACCTTACA AACACAGAGA TGATAGACTA TTGCAAGCTT TGTTCAACAT TTTAAAGAAG 
AAACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pcsk1 ( XM_018090795.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946284.1 Xenopus tropicalis proprotein convertase subtilisin/kexin type 1 (pcsk1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa42228
Clone ID Related Accession (Same CDS sequence)	XM_018090795.1 , XM_018090795.2
Accession Version	XM_018090795.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neuroendocrine convertase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018090795.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGAC GATACTGGTA CCCCGTGTGC ATTGCTTCAG TCGCTGTCTT GTCCTTGTTG 
TGTCAGGTGC TAGCGGTTGG GGTGGATAAA CGGTATGTGA ATGAATGGGC AGCAGAGATC 
CCCGGGGGGC CCGAGGAAGC CCAGTTACTT GCATTGGAGT TGGATTATGA TTACCTGGGA 
CAGATTGGAT CCTTAGAAAA TCACTACCTG TTCAGACACA AAGCTCATCC AAGAAGATCA 
CGCAGAAGTG CCCCTCATCT TACAAAGAGA CTAGCTGATG ATGGGCGGGT GTCGTGGGCG 
GAACAACAGT ACAGTAAAGA GAGAAATAAG CGGTCAACCT TCAGACAAGA CACCAAAAAC 
CTGTTTAATG ATCCCCTGTG GAATCGACAA TGGTACTTGC AAGACACAAG ATCGAATCCC 
TCATTGCCTA AGTTGGATTT ACATCTCCTT CCTGTATGGA AGAAAGGAAT AACAGGCAAA 
GGTGTTGTTA TCACAGTGCT GGATGATGGT TTGGAATGGA ACCACACTGA TATCTATGCC 
AATTACGACC CAGAAGCAAG CTATGATTTC AATGACAATG ACAATGACCC CTTTCCCAGA 
TATGATATTA CAAATGAAAA CAGACACGGG ACAAGATGTG CTGGGGAAAT TGCCATGGTT 
GCCAATAACA ATAAATGTGG AGTCGGAGTT GCATATAATG CAAAGGTTGG GGGCATACGG 
ATGCTGGATG GTGTTGTGAC AGATGCCAAA GAAGCAAGTT CCATTGGCTT CAATCCACAA 
CATGTGCATA TATACAGTGC CAGCTGGGGA CCTAATGATG ATGGCAAAAC CGTAGAAGGA 
CCGGGTAGAC TGGCACAGAA AGCTTTTGAG TATGGCATCA AACAGGGGCG AAATGGAAAA 
GGTTCCATCT TTGTTTGGGC ATCTGGAAAT GGTGGGCGAC AGGGTGATAA CTGTGACTGT 
GATGGATACA CAGACAGCAT TTACACCATC TCTATCAGCA GTGCTTCCCA GCAAGGTTTG 
TCTCCCTGGT ATGCTGAGAA ATGCTCTTCC ACCCTGGCCA CAGCATACAG CAGTGGGGAC 
TACACAGATC AGAGAATTAT AAGCGCCGAC TTACACAATG AGTGCACAGA GACGCACACC 
GGCACCTCTG CTTCAGCTCC GCTGGCTGCC GGGATCTTTG CTCTCGCACT GGAATTTAAC 
CCTGACCTTA CCTGGAGGGA TATGCAGCAC CTGGTGGTTT GGACATCAGA GTACGACCCG 
TTGGCTAACA ATGCTGGCTG GAAGAAAAAT GGCGCTGGGC TAATGGTTAA TAGCAGATTT 
GGATTTGGGC TGCTCAATGC TAAGGCACTT GTGGATTTAG CTGACCCTAA AACATGGAAA 
GGAGTTGCAG AAAAGAAGGA GTGTATAGTA GAAGATAATG AGTTTTCACC AAGGTTTCTA 
AGATCAGATG GGGAAGTAAC CATTCAAATT CCTACAAAAG CCTGTGAAGG CCAAGATAAC 
CATATCAAGT CTCTGGAGCA CTTGCAGCTT GAAGCCACAA TTGAATACAC CCGTAGGGGG 
GACCTTCACA TTACACTCAC CTCTCCATTA GGGACTAATA CAGTTCTTCT GACTGAAAGA 
GAGAGAGATA CTTCTCCATA TGGTTTCAAA AGCTGGGACT TTATGTCTGT GCATACGTGG 
GGAGAAGATC CAGCGGGAAC CTGGACGCTA AAAATCTCTG ATGTGTCTGG AAGAAAAAAA 
AATGAAGGAA GAATAGTCAG CTGGAAATTG ATTCTTCATG GAACATCGAC CCAACCGGAG 
CATATGAAAC ATCCAAGAGT TTATACTTCC TATAATGTTG TTCAAAATGA TCGAAGAGGC 
GCTGAGAAGA TGGATGACAT TGAAAAGGAA CCAATCAGTG AAAGTTTCAC AACTGAGCGG 
CCAGAAGTCA CTAAAAACAC TCCAGACAGT CAAGAAGCTG CTGAAAGGGC AAAGTCGCTA 
GCAATAGCCC ACCTTTTGAA GAATGCCTTC GACAGAGACG GAGGTTCATT TCCTCTAGAA 
GACAAAGATA TCCCTAAACA TAGTTATTTG TATGCTCTGG CCACCATTAG CAAGCAGGGA 
GAAGGAAGGG ACAGAGAGAA CGATTTGTAC AATGAGTACA TTGACAGGTT CTACCATTCC 
AGACCTTACA AACACAGAGA TGATAGACTA TTGCAAGCTT TGTTCAACAT TTTAAAGAAG 
AAACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946284.1
CDS	351..2579
Translation	MEGRYWYPVCIASVAVLSLLCQVLAVGVDKRYVNEWAAEIPGGPEEAQLLALELDYDYLGQIGSLENHYLFRHKAHPRRSRRSAPHLTKRLADDGRVSWAEQQYSKERNKRSTFRQDTKNLFNDPLWNRQWYLQDTRSNPSLPKLDLHLLPVWKKGITGKGVVITVLDDGLEWNHTDIYANYDPEASYDFNDNDNDPFPRYDITNENRHGTRCAGEIAMVANNNKCGVGVAYNAKVGGIRMLDGVVTDAKEASSIGFNPQHVHIYSASWGPNDDGKTVEGPGRLAQKAFEYGIKQGRNGKGSIFVWASGNGGRQGDNCDCDGYTDSIYTISISSASQQGLSPWYAEKCSSTLATAYSSGDYTDQRIISADLHNECTETHTGTSASAPLAAGIFALALEFNPDLTWRDMQHLVVWTSEYDPLANNAGWKKNGAGLMVNSRFGFGLLNAKALVDLADPKTWKGVAEKKECIVEDNEFSPRFLRSDGEVTIQIPTKACEGQDNHIKSLEHLQLEATIEYTRRGDLHITLTSPLGTNTVLLTERERDTSPYGFKSWDFMSVHTWGEDPAGTWTLKISDVSGRKKNEGRIVSWKLILHGTSTQPEHMKHPRVYTSYNVVQNDRRGAEKMDDIEKEPISESFTTERPEVTKNTPDSQEAAERAKSLAIAHLLKNAFDRDGGSFPLEDKDIPKHSYLYALATISKQGEGRDRENDLYNEYIDRFYHSRPYKHRDDRLLQALFNILKKKP

Target ORF information:

RefSeq Version	XM_018090795.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis proprotein convertase subtilisin/kexin type 1 (pcsk1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090795.2

ORF Insert Sequence:

ATGGAAGGAC GATACTGGTA CCCCGTGTGC ATTGCTTCAG TCGCTGTCTT GTCCTTGTTG 
TGTCAGGTGC TAGCGGTTGG GGTGGATAAA CGGTATGTGA ATGAATGGGC AGCAGAGATC 
CCCGGGGGGC CCGAGGAAGC CCAGTTACTT GCATTGGAGT TGGATTATGA TTACCTGGGA 
CAGATTGGAT CCTTAGAAAA TCACTACCTG TTCAGACACA AAGCTCATCC AAGAAGATCA 
CGCAGAAGTG CCCCTCATCT TACAAAGAGA CTAGCTGATG ATGGGCGGGT GTCGTGGGCG 
GAACAACAGT ACAGTAAAGA GAGAAATAAG CGGTCAACCT TCAGACAAGA CACCAAAAAC 
CTGTTTAATG ATCCCCTGTG GAATCGACAA TGGTACTTGC AAGACACAAG ATCGAATCCC 
TCATTGCCTA AGTTGGATTT ACATCTCCTT CCTGTATGGA AGAAAGGAAT AACAGGCAAA 
GGTGTTGTTA TCACAGTGCT GGATGATGGT TTGGAATGGA ACCACACTGA TATCTATGCC 
AATTACGACC CAGAAGCAAG CTATGATTTC AATGACAATG ACAATGACCC CTTTCCCAGA 
TATGATATTA CAAATGAAAA CAGACACGGG ACAAGATGTG CTGGGGAAAT TGCCATGGTT 
GCCAATAACA ATAAATGTGG AGTCGGAGTT GCATATAATG CAAAGGTTGG GGGCATACGG 
ATGCTGGATG GTGTTGTGAC AGATGCCAAA GAAGCAAGTT CCATTGGCTT CAATCCACAA 
CATGTGCATA TATACAGTGC CAGCTGGGGA CCTAATGATG ATGGCAAAAC CGTAGAAGGA 
CCGGGTAGAC TGGCACAGAA AGCTTTTGAG TATGGCATCA AACAGGGGCG AAATGGAAAA 
GGTTCCATCT TTGTTTGGGC ATCTGGAAAT GGTGGGCGAC AGGGTGATAA CTGTGACTGT 
GATGGATACA CAGACAGCAT TTACACCATC TCTATCAGCA GTGCTTCCCA GCAAGGTTTG 
TCTCCCTGGT ATGCTGAGAA ATGCTCTTCC ACCCTGGCCA CAGCATACAG CAGTGGGGAC 
TACACAGATC AGAGAATTAT AAGCGCCGAC TTACACAATG AGTGCACAGA GACGCACACC 
GGCACCTCTG CTTCAGCTCC GCTGGCTGCC GGGATCTTTG CTCTCGCACT GGAATTTAAC 
CCTGACCTTA CCTGGAGGGA TATGCAGCAC CTGGTGGTTT GGACATCAGA GTACGACCCG 
TTGGCTAACA ATGCTGGCTG GAAGAAAAAT GGCGCTGGGC TAATGGTTAA TAGCAGATTT 
GGATTTGGGC TGCTCAATGC TAAGGCACTT GTGGATTTAG CTGACCCTAA AACATGGAAA 
GGAGTTGCAG AAAAGAAGGA GTGTATAGTA GAAGATAATG AGTTTTCACC AAGGTTTCTA 
AGATCAGATG GGGAAGTAAC CATTCAAATT CCTACAAAAG CCTGTGAAGG CCAAGATAAC 
CATATCAAGT CTCTGGAGCA CTTGCAGCTT GAAGCCACAA TTGAATACAC CCGTAGGGGG 
GACCTTCACA TTACACTCAC CTCTCCATTA GGGACTAATA CAGTTCTTCT GACTGAAAGA 
GAGAGAGATA CTTCTCCATA TGGTTTCAAA AGCTGGGACT TTATGTCTGT GCATACGTGG 
GGAGAAGATC CAGCGGGAAC CTGGACGCTA AAAATCTCTG ATGTGTCTGG AAGAAAAAAA 
AATGAAGGAA GAATAGTCAG CTGGAAATTG ATTCTTCATG GAACATCGAC CCAACCGGAG 
CATATGAAAC ATCCAAGAGT TTATACTTCC TATAATGTTG TTCAAAATGA TCGAAGAGGC 
GCTGAGAAGA TGGATGACAT TGAAAAGGAA CCAATCAGTG AAAGTTTCAC AACTGAGCGG 
CCAGAAGTCA CTAAAAACAC TCCAGACAGT CAAGAAGCTG CTGAAAGGGC AAAGTCGCTA 
GCAATAGCCC ACCTTTTGAA GAATGCCTTC GACAGAGACG GAGGTTCATT TCCTCTAGAA 
GACAAAGATA TCCCTAAACA TAGTTATTTG TATGCTCTGG CCACCATTAG CAAGCAGGGA 
GAAGGAAGGG ACAGAGAGAA CGATTTGTAC AATGAGTACA TTGACAGGTT CTACCATTCC 
AGACCTTACA AACACAGAGA TGATAGACTA TTGCAAGCTT TGTTCAACAT TTTAAAGAAG 
AAACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pcsk1 ( XM_004910458.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910515.3 Xenopus tropicalis proprotein convertase subtilisin/kexin type 1 (pcsk1), transcript variant X1, mRNA.

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CloneID	OXa49067
Clone ID Related Accession (Same CDS sequence)	XM_004910458.3
Accession Version	XM_004910458.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neuroendocrine convertase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910458.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTGCC TTTGTGTGTG GGCGTCTCCA GCTGTAATGA GTGAGCTCAG TGTAGTGGAG 
CTGCGAGCAC CCAGCACTTG CTATTTTAGC CTCTCACCTC CCTCGGGAAT ACCGTATAGA 
TTGAAGACGC CTGGAAGTGT GTTTGACGTC GTTGTGACGT GTAAAAGACA CATTTCCAAT 
GAAGCTGCTA AAGGACCGAG CATTTTTCTA GCCTCGGAGA GAGAGAGAAA CCTGCTGCTC 
TTATCTGTTC GCATAGCAAC CAGGACCAAG CACCCCGATT TGAAATTCTC ATTGGACTCT 
TTTAGGGGTT TTGGCTCAGA CACTGTGAGA ATGGAAGGAC GATACTGGTA CCCCATGTGC 
ATTGCTTCAG TCGCTGTCTT GTCCTTGTTG TGTCAGGTGC TAGCTGCTGG GGTGGATAAA 
CGGTATATGA ATGAATGGGC AGCAGAGATC CCCGGGGGGC CCGAGGAAGC CCAGTTACTT 
GCATTGGAGT TGGATTATGA TTACCTGGGA CAGATTGGAT CCTTAGAAAA TCACTACCTG 
TTCAGACACA AAGCTCATCC AAGAAGATCA CGCAGAAGTG CCCCTCATCT TACAAAGAGA 
CTAGCTGATG ATGGGCGGGT GTCGTGGGCG GAACAACAGT ACAGTAAAGA GAGAAATAAG 
CGGTCAACCT TCAGACAAGA CACCAAAAAC CTGTTTAATG ATCCCCTGTG GAATCGACAA 
TGGTACTTGC AAGACACAAG ATCGAATCCC TCATTGCCTA AGTTGGATTT ACATCTCCTT 
CCTGTATGGA AGAAAGGAAT AACAGGCAAA GGTGTTGTTA TCACAGTGCT GGATGATGGT 
TTGGAATGGA ACCACACTGA TATCTATGCC AATTACGACC CAGAAGCAAG CTATGATTTC 
AATGACAATG ACAATGACCC CTTTCCCAGA TATGATATTA CAAATGAAAA CAGACACGGG 
ACAAGATGTG CTGGGGAAAT TGCCATGGTT GCCAATAACA ATAAATGTGG AGTCGGAGTT 
GCATATAATG CAAAGGTTGG GGGCATACGG ATGCTGGATG GTGTTGTGAC AGATGCCAAA 
GAAGCAAGTT CCATTGGCTT CAATCCACAA CATGTGCATA TATACAGTGC CAGCTGGGGA 
CCTAATGATG ATGGCAAAAC CGTAGAAGGA CCGGGTAGAC TGGCACAGAA AGCTTTTGAG 
TATGGCATCA AACAGGGGCG AAATGGAAAA GGTTCCATCT TTGTTTGGGC ATCTGGAAAT 
GGTGGGCGAC AGGGTGATAA CTGTGACTGT GATGGATACA CAGACAGCAT TTACACCATC 
TCTATCAGCA GTGCTTCCCA GCAAGGTTTG TCTCCCTGGT ATGCTGAGAA ATGCTCTTCC 
ACCCTGGCCA CAGCATACAG CAGTGGGGAC TACACAGATC AGAGAATTAT AAGCGCCGAC 
TTACACAATG AGTGCACAGA GACGCACACC GGCACCTCTG CTTCAGCTCC GCTGGCTGCC 
GGGATCTTTG CTCTCGCACT GGAATTTAAC CCTGACCTTA CCTGGAGGGA TATGCAGCAC 
CTGGTGGTTT GGACATCAGA GTACGACCCG TTGGCTAACA ATGCTGGCTG GAAGAAAAAT 
GGCGCTGGGC TAATGGTTAA TAGCAGATTT GGATTTGGGC TGCTCAATGC TAAGGCACTT 
GTGGATTTAG CTGACCCTAA AACATGGAAA GGAGTTGCAG AAAAGAAGGA GTGTATAGTA 
GAAGATAATG AGTTTTCACC AAGGTTTCTA AGATCAGATG GGGAAGTAAC CATTCAAATT 
CCTACAAAAG CCTGTGAAGG CCAAGATAAC CATATCAAGT CTCTGGAGCA CTTGCAGCTT 
GAAGCCACAA TTGAATACAC CCGTAGGGGG GACCTTCACA TTACACTCAC CTCTCCATTA 
GGGACTAATA CAGTTCTTCT GACTGAAAGA GAGAGAGATA CTTCTCCATA TGGTTTCAAA 
AGCTGGGACT TTATGTCTGT GCATACGTGG GGAGAAGATC CAGCGGGAAC CTGGACGCTA 
AAAATCTCTG ATGTGTCTGG AAGAAAAAAA AATGAAGGAA GAATAGTCAG CTGGAAATTG 
ATTCTTCATG GAACATCGAC CCAACCGGAG CATATGAAAC ATCCAAGAGT TTATACTTCC 
TATAATGTTG TTCAAAATGA TCGAAGAGGC GCTGAGAAGA TGGATGACAT TGAAAAGGAA 
CCAATCAGTG AAAGTTTCAC AACTGAGCGG CCAGAAGTCA CTAAAAACAC TCCAGACAGT 
CAAGAAGCTG CTGAAAGGGC AAAGTCGCTA GCAATAGCCC ACCTTTTGAA GAATGCCTTC 
GACAGAGACG GAGGTTCATT TCCTCTAGAA GACAAAGATA TCCCTAAACA TAGTTATTTG 
TATGCTCTGG CCACCATTAG CAAGCAGGGA GAAGGAAGGG ACAGAGAGAA CGATTTGTAC 
AATGAGTACA TTGACAGGTT CTACCATTCC AGACCTTACA AACACAGAGA TGATAGACTA 
TTGCAAGCTT TGTTCAACAT TTTAAAGAAG AAACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910515.3
CDS	24..2582
Translation	MICLCVWASPAVMSELSVVELRAPSTCYFSLSPPSGIPYRLKTPGSVFDVVVTCKRHISNEAAKGPSIFLASERERNLLLLSVRIATRTKHPDLKFSLDSFRGFGSDTVRMEGRYWYPMCIASVAVLSLLCQVLAAGVDKRYMNEWAAEIPGGPEEAQLLALELDYDYLGQIGSLENHYLFRHKAHPRRSRRSAPHLTKRLADDGRVSWAEQQYSKERNKRSTFRQDTKNLFNDPLWNRQWYLQDTRSNPSLPKLDLHLLPVWKKGITGKGVVITVLDDGLEWNHTDIYANYDPEASYDFNDNDNDPFPRYDITNENRHGTRCAGEIAMVANNNKCGVGVAYNAKVGGIRMLDGVVTDAKEASSIGFNPQHVHIYSASWGPNDDGKTVEGPGRLAQKAFEYGIKQGRNGKGSIFVWASGNGGRQGDNCDCDGYTDSIYTISISSASQQGLSPWYAEKCSSTLATAYSSGDYTDQRIISADLHNECTETHTGTSASAPLAAGIFALALEFNPDLTWRDMQHLVVWTSEYDPLANNAGWKKNGAGLMVNSRFGFGLLNAKALVDLADPKTWKGVAEKKECIVEDNEFSPRFLRSDGEVTIQIPTKACEGQDNHIKSLEHLQLEATIEYTRRGDLHITLTSPLGTNTVLLTERERDTSPYGFKSWDFMSVHTWGEDPAGTWTLKISDVSGRKKNEGRIVSWKLILHGTSTQPEHMKHPRVYTSYNVVQNDRRGAEKMDDIEKEPISESFTTERPEVTKNTPDSQEAAERAKSLAIAHLLKNAFDRDGGSFPLEDKDIPKHSYLYALATISKQGEGRDRENDLYNEYIDRFYHSRPYKHRDDRLLQALFNILKKKP

Target ORF information:

RefSeq Version	XM_004910458.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis proprotein convertase subtilisin/kexin type 1 (pcsk1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910458.3

ORF Insert Sequence:

ATGATTTGCC TTTGTGTGTG GGCGTCTCCA GCTGTAATGA GTGAGCTCAG TGTAGTGGAG 
CTGCGAGCAC CCAGCACTTG CTATTTTAGC CTCTCACCTC CCTCGGGAAT ACCGTATAGA 
TTGAAGACGC CTGGAAGTGT GTTTGACGTC GTTGTGACGT GTAAAAGACA CATTTCCAAT 
GAAGCTGCTA AAGGACCGAG CATTTTTCTA GCCTCGGAGA GAGAGAGAAA CCTGCTGCTC 
TTATCTGTTC GCATAGCAAC CAGGACCAAG CACCCCGATT TGAAATTCTC ATTGGACTCT 
TTTAGGGGTT TTGGCTCAGA CACTGTGAGA ATGGAAGGAC GATACTGGTA CCCCATGTGC 
ATTGCTTCAG TCGCTGTCTT GTCCTTGTTG TGTCAGGTGC TAGCTGCTGG GGTGGATAAA 
CGGTATATGA ATGAATGGGC AGCAGAGATC CCCGGGGGGC CCGAGGAAGC CCAGTTACTT 
GCATTGGAGT TGGATTATGA TTACCTGGGA CAGATTGGAT CCTTAGAAAA TCACTACCTG 
TTCAGACACA AAGCTCATCC AAGAAGATCA CGCAGAAGTG CCCCTCATCT TACAAAGAGA 
CTAGCTGATG ATGGGCGGGT GTCGTGGGCG GAACAACAGT ACAGTAAAGA GAGAAATAAG 
CGGTCAACCT TCAGACAAGA CACCAAAAAC CTGTTTAATG ATCCCCTGTG GAATCGACAA 
TGGTACTTGC AAGACACAAG ATCGAATCCC TCATTGCCTA AGTTGGATTT ACATCTCCTT 
CCTGTATGGA AGAAAGGAAT AACAGGCAAA GGTGTTGTTA TCACAGTGCT GGATGATGGT 
TTGGAATGGA ACCACACTGA TATCTATGCC AATTACGACC CAGAAGCAAG CTATGATTTC 
AATGACAATG ACAATGACCC CTTTCCCAGA TATGATATTA CAAATGAAAA CAGACACGGG 
ACAAGATGTG CTGGGGAAAT TGCCATGGTT GCCAATAACA ATAAATGTGG AGTCGGAGTT 
GCATATAATG CAAAGGTTGG GGGCATACGG ATGCTGGATG GTGTTGTGAC AGATGCCAAA 
GAAGCAAGTT CCATTGGCTT CAATCCACAA CATGTGCATA TATACAGTGC CAGCTGGGGA 
CCTAATGATG ATGGCAAAAC CGTAGAAGGA CCGGGTAGAC TGGCACAGAA AGCTTTTGAG 
TATGGCATCA AACAGGGGCG AAATGGAAAA GGTTCCATCT TTGTTTGGGC ATCTGGAAAT 
GGTGGGCGAC AGGGTGATAA CTGTGACTGT GATGGATACA CAGACAGCAT TTACACCATC 
TCTATCAGCA GTGCTTCCCA GCAAGGTTTG TCTCCCTGGT ATGCTGAGAA ATGCTCTTCC 
ACCCTGGCCA CAGCATACAG CAGTGGGGAC TACACAGATC AGAGAATTAT AAGCGCCGAC 
TTACACAATG AGTGCACAGA GACGCACACC GGCACCTCTG CTTCAGCTCC GCTGGCTGCC 
GGGATCTTTG CTCTCGCACT GGAATTTAAC CCTGACCTTA CCTGGAGGGA TATGCAGCAC 
CTGGTGGTTT GGACATCAGA GTACGACCCG TTGGCTAACA ATGCTGGCTG GAAGAAAAAT 
GGCGCTGGGC TAATGGTTAA TAGCAGATTT GGATTTGGGC TGCTCAATGC TAAGGCACTT 
GTGGATTTAG CTGACCCTAA AACATGGAAA GGAGTTGCAG AAAAGAAGGA GTGTATAGTA 
GAAGATAATG AGTTTTCACC AAGGTTTCTA AGATCAGATG GGGAAGTAAC CATTCAAATT 
CCTACAAAAG CCTGTGAAGG CCAAGATAAC CATATCAAGT CTCTGGAGCA CTTGCAGCTT 
GAAGCCACAA TTGAATACAC CCGTAGGGGG GACCTTCACA TTACACTCAC CTCTCCATTA 
GGGACTAATA CAGTTCTTCT GACTGAAAGA GAGAGAGATA CTTCTCCATA TGGTTTCAAA 
AGCTGGGACT TTATGTCTGT GCATACGTGG GGAGAAGATC CAGCGGGAAC CTGGACGCTA 
AAAATCTCTG ATGTGTCTGG AAGAAAAAAA AATGAAGGAA GAATAGTCAG CTGGAAATTG 
ATTCTTCATG GAACATCGAC CCAACCGGAG CATATGAAAC ATCCAAGAGT TTATACTTCC 
TATAATGTTG TTCAAAATGA TCGAAGAGGC GCTGAGAAGA TGGATGACAT TGAAAAGGAA 
CCAATCAGTG AAAGTTTCAC AACTGAGCGG CCAGAAGTCA CTAAAAACAC TCCAGACAGT 
CAAGAAGCTG CTGAAAGGGC AAAGTCGCTA GCAATAGCCC ACCTTTTGAA GAATGCCTTC 
GACAGAGACG GAGGTTCATT TCCTCTAGAA GACAAAGATA TCCCTAAACA TAGTTATTTG 
TATGCTCTGG CCACCATTAG CAAGCAGGGA GAAGGAAGGG ACAGAGAGAA CGATTTGTAC 
AATGAGTACA TTGACAGGTT CTACCATTCC AGACCTTACA AACACAGAGA TGATAGACTA 
TTGCAAGCTT TGTTCAACAT TTTAAAGAAG AAACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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pcsk1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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