plcg2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	plcg2
Entrez Gene ID	100496686
Full Name	phospholipase C gamma 2
General protein information	Preferred Names phospholipase C gamma 2 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 phospholipase C, gamma 2 (phosphatidylinositol-specific)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018093522.1	XP_017949011.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 isoform X1
XM_012962308.2	XP_012817762.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 isoform X3
XM_018093520.1	XP_017949009.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 isoform X1
XM_018093520.2	XP_017949009.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 isoform X1
XM_012962308.3	XP_012817762.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 isoform X3
XM_002934948.4	XP_002934994.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 isoform X2
XM_018093522.2	XP_017949011.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 isoform X1
XM_002934948.5	XP_002934994.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PLCG2	XP_004944314.1
Homo sapiens (human)	PLCG2	NP_002652.2
Pan troglodytes (chimpanzee)	PLCG2	XP_001148089.1
	PLCG2	XP_001111717.1
Mus musculus (house mouse)	Plcg2	NP_758489.1
Arabidopsis thaliana (thale cress)	PLC5	NP_200677.2
Xenopus tropicalis (tropical clawed frog)	plcg2	XP_002934994.2
Bos taurus (cattle)	PLCG2	XP_003587251.2
Danio rerio (zebrafish)	plcg2	NP_001038433.1
Canis lupus familiaris (dog)	PLCG2	XP_005620713.1
Rattus norvegicus (Norway rat)	Plcg2	NP_058864.1

Gene Ontology

The following plcg2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the plcg2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa08886	XM_002934948.5 Latest version!	Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa36654	XM_018093520.2 Latest version!	Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa36654	XM_018093522.2 Latest version!	Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa36655	XM_012962308.3 Latest version!	Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OXa36654	XM_018093520.1	Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa36655	XM_012962308.2	Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa36654	XM_018093522.1	Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa08886	XM_002934948.4	Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

plcg2 ( XM_002934948.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934994.2 Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa08886
Clone ID Related Accession (Same CDS sequence)	XM_002934948.4 , XM_002934948.5
Accession Version	XM_002934948.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934948.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGCA GGGGGAGTTC TGATTATAGA AGTCTCCCTG AATATGAGAA GAGCCAGATT 
AAGAGAACCC TTGAGCTGGG CACAGTCATG ACTGTCTTCA ACTACAAGAA GTCACAGCCT 
GATCGAAGGA CCATCCAAGT GATAATGGAA ACTGGCCAGG TGGCGTGGTG TAAGACAAAT 
GATAAGATTG AAGGATTCTT GGACCTAATG GAAATAAAGG AAATTCGTCC AGGGAAAAAT 
TCAAAGGATT TTCAACGTGG AAAATGTGAC CGTTGTAAAG AAGACGTTTG CTTCACTATT 
TTTTATGGGA ACCAATTTGT TCTGAATACT TTGAGTTTAG CAGCCGATTC AGTTGACGAT 
GCTGAAAAAT GGAAATGTGG TTTAACTATC TTACTTGAAG AAGTTGCTTC TGCCTCAACA 
CCAGCAATAA TTGAAAGTTG GCTGAGAAAA CAGATATACT CTGTGGATCA GACTAGAAAG 
AACAGTATTA GCTTCCGGGA ACTGAAGACC ATTCTTTCAC AGGCCAACTT CAAAGCTAAT 
AGCATGAGAG TGCTGAAGGA CAAGTTAACG GAGATTGGCA TTCAAAGGGA TGATATTAGT 
TTTGAACATT TTCACGTATT CTACAAAAAG CTCATGTTTG AACAGCAGAA GATGATACTG 
GATGAATTTC GGAAGGATTC TTCAGTGTTT ATTTTGGGAA ATGTTGACCG CCCAGATGGG 
TCAGCTGTCC ACCTGCATGA TTTTCAAAGG TTTCTAGTGC ATGAGCAACA GGAATCCCCA 
GCTCAAGATC TCAGTAAGGT TCGTGAACGG ATGACAAAAT TTGTAGATGA TACTATGAGG 
GAAACAGCTG AACCATTTCT TTTTGTGGAC GAGTTCTTGA CCTACTTATT TTCGAAAGAA 
AACAGCATTT GGGATGAGAA ATATGACACG ATCAGTGTTC AAAATATGAA TAATCCTCTT 
TCACATTACT GGATTGCTTC CTCACATAAT ACGTATTTAA CAGGGGACCA GCTGCGGAGT 
GAATCATCTA CTGAAGCATA TGTGCGCTGT CTGCGCATGG GATGCAGGTG CATTGAATTG 
GACTGCTGGG ATGGACCTGA TGGAAAGCCA ATTATTTATC ATGGATGGAC ACGAACAACA 
AAAATTAAAT TTGATGATGT GGTACAGGCT ATTAGGGACC ATGCATTTGC TACATCTGAG 
TATCCAGTTA TCCTTTCTAT AGAGGAACAT TGCTGTGTTG AACAACAGAG ACACATGGCC 
AAAGTTTTTA AAGAAGTATT AGGAGAGCTT CTTTTGACTA AACCAATAGA AGCTACTGCC 
GATCAGTTGC CTTCTCCCAC ACAACTTAAA GGGAGGATCA TTATTAAGCA TAAAAAACTG 
GAAACAAAGA ATCCTGGGAC AAACACTGAG AATCATAAAG AAGAACAAAA TCTTCAGGGT 
GAGCTGTACA TGTGGGATGG TATTGACCAG AAATGGTCTT CTCGTTACTG TGCTATTGCT 
GGTGATAAAT TATCTTATGG AGATGAAATT GAACAGACTG AAGACAGCGC AGCACCAAAG 
AGAACAATAG ATTTACATCT ACAAGAGAAA TGGTTCCACG GGCGAATGTA TAATGGGCGT 
TTCATGGCGG AAAAGCTACT TCAGGAGTAT TGTGCAGAAG TGGGAGGCAA AGATGGGACT 
TTCTTAGTAC GACAAAGTGA ATCTTATCGT GATGATTACA CACTCTCTTT CTGGAGGTCA 
GGCAGGGTCC AACACTGCCG TATTCGTTCC TGTACAGATG GAGAAATCAT AAAGTATTTC 
CTGACCGACA ACCTAATGTT TGATAGTATC TATGATTTGA TTCAACACTA CAAAGAGTAC 
TCATTGCGCT GTGCAGAATT TGAGCTGCGA CTAACAGATG CTGTTCCTCA ACCCAACCCT 
CACTTATCCA AAGACTGGTA CTATGATAAC CTCAGCAGAG GAGAGGCAGA AGATATGTTA 
ATGAGAATCC CTCGGGATGG GGCCTTTTTA ATAAGAAGAA GAGAGGAACA AGATTCCTAT 
GCAATTACTT TTAGAGCTGA AGGGAAAGTG AAGCACTGTA GAATACAAAG GGATGGACGC 
ATGTTTGTAC TAGGAACTTC TGCATACTTT GAAAACCTTG TGGAACTTGT CAGCTATTAC 
CAGAAACACC CGCTATACCG AAAAATGAAA CTACGCTATC CAGTTACAGA AGAGTTACTT 
CAGAGATATA GCACAGAAAA GGATTTAAGT GCCATATATG ATGTAAAGAA TAAACTGTAT 
GTCAATCCTG AAGAAATTTC TCCAGCAATG CCCCAAGGAA CAGTGAAAGC TTTGTACAAC 
TATACAGCTG TAAGATCAGA TGAGCTGAGC TTCTGCACAG GTGCCCTGAT TCATAATGTC 
TCAAGGGTCT CTGAAGGATG GTGGAAAGGA GATTATGGAG ACAAGATACA GTATTTTTTC 
CCCTCAAATT ATGTGGAGGA CATATCAATG AATGCTTCTG AAATAATTGC AAATCAGCTT 
ATTGAAGACA GTCCATTGGG GTCCATGTGC AGAGGTATCC TGGAAATGGG ATCATATAAT 
ATCAGGAAGT TGCTTAATGG AAAATATAAC AAACAGTTTG TTTTGTCCCT GGAATCCAAG 
AATCCAGAAG AGCCTACTGT AGATTTTGCA GCAGATACAA TAGAAGAGTT TTTTGTGTGG 
TACAAACACA TCCAACAGAT TGAACAGAAA AAGGGAGCAG AGGACTTTGA AAAGGGAACA 
CTGGAGAAGC AGCAAGAGAT TGCCCGAGAA TTATCTGATC TGGTTATGTA CTGTAAGCCC 
AAAAGTGAAG CTAAGGAGAA ATTAGAGTCG CCGGATTTTC GGGAAATTCG ATCATTTGCA 
GAGAATAAGG GAGAGAGTAT TGCTCGACAA AAATCCATTG AGTTGTTGCG GTATAACCGT 
AAAGCCTTTA CGCGAATCTA CCCTAAGTCC TCAAGAGTTG ACTCTTCAAA CTATGACCCC 
CTACCCCTAT GGCTCTGTGG AGACCAGATG ATTGCACTAA ATTACCAGAC TCCAGATAGG 
TACATGCAAA TAAATAATGC AATGTTCGCT TACAATGGAG AATGCGGCTA CATCCTGCAG 
CCAGAGTGTA TGAGGGAAGA GTCATATGAC CCCAGTGACT CTAGAAGACA GCTCCAAATG 
ATTGTGACAA TTAAGGTCTT AGGAGCTCGA CATTTGCCAA AACCAGGGAG AGGTATTGCA 
TGCCCTTTTG TAGAAGTGGA GGTATGCGGT GGGGAATATG ACAACAATAA GTTCAGAACA 
ATTGTTATGA ATGATAATGG CCTCAACCCT GTATGGTCAG GAAGTTCTCA ACAGGCTGAG 
TTTGAGATCC ATGAGCCTGA GATTGCCTTT CTGAGGTTTG TAGTATATGA AGAAGATATG 
TTCAGTGACC CCAACTTCTT AGCTCAGTGC ACCATCCCAG TTAAATGTTT AAAAACAGGT 
TATAGATCAG TTCCACTAAA AAACGGTTAC AGTGAAAACC TAGAGCTTGC TTCTCTGTTG 
GTTTATTGCC AAATACAAAA AGATCTGGAA AAAGTAGAAG AACTGTACTC TTCATCAAAG 
CAACTCCGCA AGCGCCAGAC AGACCTGACC AATGAACTGT TTCTTTACGA CACTCACCAA 
AACATGAGAC ACCCCAGTCA TGAGGCATTT GTGAACGACA GTAAAGGGAA TGCCAGTCCA 
GAGGTGTCCA ACAGGAGGTT AAAGGAAAGA CAGATCAGTA ACAGCAAATT CTACTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934994.2
CDS	269..4048
Translation	MDRRGSSDYRSLPEYEKSQIKRTLELGTVMTVFNYKKSQPDRRTIQVIMETGQVAWCKTNDKIEGFLDLMEIKEIRPGKNSKDFQRGKCDRCKEDVCFTIFYGNQFVLNTLSLAADSVDDAEKWKCGLTILLEEVASASTPAIIESWLRKQIYSVDQTRKNSISFRELKTILSQANFKANSMRVLKDKLTEIGIQRDDISFEHFHVFYKKLMFEQQKMILDEFRKDSSVFILGNVDRPDGSAVHLHDFQRFLVHEQQESPAQDLSKVRERMTKFVDDTMRETAEPFLFVDEFLTYLFSKENSIWDEKYDTISVQNMNNPLSHYWIASSHNTYLTGDQLRSESSTEAYVRCLRMGCRCIELDCWDGPDGKPIIYHGWTRTTKIKFDDVVQAIRDHAFATSEYPVILSIEEHCCVEQQRHMAKVFKEVLGELLLTKPIEATADQLPSPTQLKGRIIIKHKKLETKNPGTNTENHKEEQNLQGELYMWDGIDQKWSSRYCAIAGDKLSYGDEIEQTEDSAAPKRTIDLHLQEKWFHGRMYNGRFMAEKLLQEYCAEVGGKDGTFLVRQSESYRDDYTLSFWRSGRVQHCRIRSCTDGEIIKYFLTDNLMFDSIYDLIQHYKEYSLRCAEFELRLTDAVPQPNPHLSKDWYYDNLSRGEAEDMLMRIPRDGAFLIRRREEQDSYAITFRAEGKVKHCRIQRDGRMFVLGTSAYFENLVELVSYYQKHPLYRKMKLRYPVTEELLQRYSTEKDLSAIYDVKNKLYVNPEEISPAMPQGTVKALYNYTAVRSDELSFCTGALIHNVSRVSEGWWKGDYGDKIQYFFPSNYVEDISMNASEIIANQLIEDSPLGSMCRGILEMGSYNIRKLLNGKYNKQFVLSLESKNPEEPTVDFAADTIEEFFVWYKHIQQIEQKKGAEDFEKGTLEKQQEIARELSDLVMYCKPKSEAKEKLESPDFREIRSFAENKGESIARQKSIELLRYNRKAFTRIYPKSSRVDSSNYDPLPLWLCGDQMIALNYQTPDRYMQINNAMFAYNGECGYILQPECMREESYDPSDSRRQLQMIVTIKVLGARHLPKPGRGIACPFVEVEVCGGEYDNNKFRTIVMNDNGLNPVWSGSSQQAEFEIHEPEIAFLRFVVYEEDMFSDPNFLAQCTIPVKCLKTGYRSVPLKNGYSENLELASLLVYCQIQKDLEKVEELYSSSKQLRKRQTDLTNELFLYDTHQNMRHPSHEAFVNDSKGNASPEVSNRRLKERQISNSKFYS

Target ORF information:

RefSeq Version	XM_002934948.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934948.5

ORF Insert Sequence:

ATGGATCGCA GGGGGAGTTC TGATTATAGA AGTCTCCCTG AATATGAGAA GAGCCAGATT 
AAGAGAACCC TTGAGCTGGG CACAGTCATG ACTGTCTTCA ACTACAAGAA GTCACAGCCT 
GATCGAAGGA CCATCCAAGT GATAATGGAA ACTGGCCAGG TGGCGTGGTG TAAGACAAAT 
GATAAGATTG AAGGATTCTT GGACCTAATG GAAATAAAGG AAATTCGTCC AGGGAAAAAT 
TCAAAGGATT TTCAACGTGG AAAATGTGAC CGTTGTAAAG AAGACGTTTG CTTCACTATT 
TTTTATGGGA ACCAATTTGT TCTGAATACT TTGAGTTTAG CAGCCGATTC AGTTGACGAT 
GCTGAAAAAT GGAAATGTGG TTTAACTATC TTACTTGAAG AAGTTGCTTC TGCCTCAACA 
CCAGCAATAA TTGAAAGTTG GCTGAGAAAA CAGATATACT CTGTGGATCA GACTAGAAAG 
AACAGTATTA GCTTCCGGGA ACTGAAGACC ATTCTTTCAC AGGCCAACTT CAAAGCTAAT 
AGCATGAGAG TGCTGAAGGA CAAGTTAACG GAGATTGGCA TTCAAAGGGA TGATATTAGT 
TTTGAACATT TTCACGTATT CTACAAAAAG CTCATGTTTG AACAGCAGAA GATGATACTG 
GATGAATTTC GGAAGGATTC TTCAGTGTTT ATTTTGGGAA ATGTTGACCG CCCAGATGGG 
TCAGCTGTCC ACCTGCATGA TTTTCAAAGG TTTCTAGTGC ATGAGCAACA GGAATCCCCA 
GCTCAAGATC TCAGTAAGGT TCGTGAACGG ATGACAAAAT TTGTAGATGA TACTATGAGG 
GAAACAGCTG AACCATTTCT TTTTGTGGAC GAGTTCTTGA CCTACTTATT TTCGAAAGAA 
AACAGCATTT GGGATGAGAA ATATGACACG ATCAGTGTTC AAAATATGAA TAATCCTCTT 
TCACATTACT GGATTGCTTC CTCACATAAT ACGTATTTAA CAGGGGACCA GCTGCGGAGT 
GAATCATCTA CTGAAGCATA TGTGCGCTGT CTGCGCATGG GATGCAGGTG CATTGAATTG 
GACTGCTGGG ATGGACCTGA TGGAAAGCCA ATTATTTATC ATGGATGGAC ACGAACAACA 
AAAATTAAAT TTGATGATGT GGTACAGGCT ATTAGGGACC ATGCATTTGC TACATCTGAG 
TATCCAGTTA TCCTTTCTAT AGAGGAACAT TGCTGTGTTG AACAACAGAG ACACATGGCC 
AAAGTTTTTA AAGAAGTATT AGGAGAGCTT CTTTTGACTA AACCAATAGA AGCTACTGCC 
GATCAGTTGC CTTCTCCCAC ACAACTTAAA GGGAGGATCA TTATTAAGCA TAAAAAACTG 
GAAACAAAGA ATCCTGGGAC AAACACTGAG AATCATAAAG AAGAACAAAA TCTTCAGGGT 
GAGCTGTACA TGTGGGATGG TATTGACCAG AAATGGTCTT CTCGTTACTG TGCTATTGCT 
GGTGATAAAT TATCTTATGG AGATGAAATT GAACAGACTG AAGACAGCGC AGCACCAAAG 
AGAACAATAG ATTTACATCT ACAAGAGAAA TGGTTCCACG GGCGAATGTA TAATGGGCGT 
TTCATGGCGG AAAAGCTACT TCAGGAGTAT TGTGCAGAAG TGGGAGGCAA AGATGGGACT 
TTCTTAGTAC GACAAAGTGA ATCTTATCGT GATGATTACA CACTCTCTTT CTGGAGGTCA 
GGCAGGGTCC AACACTGCCG TATTCGTTCC TGTACAGATG GAGAAATCAT AAAGTATTTC 
CTGACCGACA ACCTAATGTT TGATAGTATC TATGATTTGA TTCAACACTA CAAAGAGTAC 
TCATTGCGCT GTGCAGAATT TGAGCTGCGA CTAACAGATG CTGTTCCTCA ACCCAACCCT 
CACTTATCCA AAGACTGGTA CTATGATAAC CTCAGCAGAG GAGAGGCAGA AGATATGTTA 
ATGAGAATCC CTCGGGATGG GGCCTTTTTA ATAAGAAGAA GAGAGGAACA AGATTCCTAT 
GCAATTACTT TTAGAGCTGA AGGGAAAGTG AAGCACTGTA GAATACAAAG GGATGGACGC 
ATGTTTGTAC TAGGAACTTC TGCATACTTT GAAAACCTTG TGGAACTTGT CAGCTATTAC 
CAGAAACACC CGCTATACCG AAAAATGAAA CTACGCTATC CAGTTACAGA AGAGTTACTT 
CAGAGATATA GCACAGAAAA GGATTTAAGT GCCATATATG ATGTAAAGAA TAAACTGTAT 
GTCAATCCTG AAGAAATTTC TCCAGCAATG CCCCAAGGAA CAGTGAAAGC TTTGTACAAC 
TATACAGCTG TAAGATCAGA TGAGCTGAGC TTCTGCACAG GTGCCCTGAT TCATAATGTC 
TCAAGGGTCT CTGAAGGATG GTGGAAAGGA GATTATGGAG ACAAGATACA GTATTTTTTC 
CCCTCAAATT ATGTGGAGGA CATATCAATG AATGCTTCTG AAATAATTGC AAATCAGCTT 
ATTGAAGACA GTCCATTGGG GTCCATGTGC AGAGGTATCC TGGAAATGGG ATCATATAAT 
ATCAGGAAGT TGCTTAATGG AAAATATAAC AAACAGTTTG TTTTGTCCCT GGAATCCAAG 
AATCCAGAAG AGCCTACTGT AGATTTTGCA GCAGATACAA TAGAAGAGTT TTTTGTGTGG 
TACAAACACA TCCAACAGAT TGAACAGAAA AAGGGAGCAG AGGACTTTGA AAAGGGAACA 
CTGGAGAAGC AGCAAGAGAT TGCCCGAGAA TTATCTGATC TGGTTATGTA CTGTAAGCCC 
AAAAGTGAAG CTAAGGAGAA ATTAGAGTCG CCGGATTTTC GGGAAATTCG ATCATTTGCA 
GAGAATAAGG GAGAGAGTAT TGCTCGACAA AAATCCATTG AGTTGTTGCG GTATAACCGT 
AAAGCCTTTA CGCGAATCTA CCCTAAGTCC TCAAGAGTTG ACTCTTCAAA CTATGACCCC 
CTACCCCTAT GGCTCTGTGG AGACCAGATG ATTGCACTAA ATTACCAGAC TCCAGATAGG 
TACATGCAAA TAAATAATGC AATGTTCGCT TACAATGGAG AATGCGGCTA CATCCTGCAG 
CCAGAGTGTA TGAGGGAAGA GTCATATGAC CCCAGTGACT CTAGAAGACA GCTCCAAATG 
ATTGTGACAA TTAAGGTCTT AGGAGCTCGA CATTTGCCAA AACCAGGGAG AGGTATTGCA 
TGCCCTTTTG TAGAAGTGGA GGTATGCGGT GGGGAATATG ACAACAATAA GTTCAGAACA 
ATTGTTATGA ATGATAATGG CCTCAACCCT GTATGGTCAG GAAGTTCTCA ACAGGCTGAG 
TTTGAGATCC ATGAGCCTGA GATTGCCTTT CTGAGGTTTG TAGTATATGA AGAAGATATG 
TTCAGTGACC CCAACTTCTT AGCTCAGTGC ACCATCCCAG TTAAATGTTT AAAAACAGGT 
TATAGATCAG TTCCACTAAA AAACGGTTAC AGTGAAAACC TAGAGCTTGC TTCTCTGTTG 
GTTTATTGCC AAATACAAAA AGATCTGGAA AAAGTAGAAG AACTGTACTC TTCATCAAAG 
CAACTCCGCA AGCGCCAGAC AGACCTGACC AATGAACTGT TTCTTTACGA CACTCACCAA 
AACATGAGAC ACCCCAGTCA TGAGGCATTT GTGAACGACA GTAAAGGGAA TGCCAGTCCA 
GAGGTGTCCA ACAGGAGGTT AAAGGAAAGA CAGATCAGTA ACAGCAAATT CTACTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcg2 ( XM_018093520.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949009.1 Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa36654
Clone ID Related Accession (Same CDS sequence)	XM_018093522.1 , XM_018093520.1 , XM_018093520.2 , XM_018093522.2
Accession Version	XM_018093520.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018093520.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGCA GGGGGAGTTC TGATTATAGA AGTCTCCCTG AATATGAGAA GAGCCAGATT 
AAGAGAACCC TTGAGCTGGG CACAGTCATG ACTGTCTTCA ACTACAAGAA GTCACAGCCT 
GATCGAAGGA CCATCCAAGT GATAATGGAA ACTGGCCAGG TGGCGTGGTG TAAGACAAAT 
GATAAGATTG AAGGATTCTT GGACCTAATG GAAATAAAGG AAATTCGTCC AGGGAAAAAT 
TCAAAGGATT TTCAACGTGG AAAATGTGAC CGTTGTAAAG AAGACGTTTG CTTCACTATT 
TTTTATGGGA ACCAATTTGT TCTGAATACT TTGAGTTTAG CAGCCGATTC AGTTGACGAT 
GCTGAAAAAT GGAAATGTGG TTTAACTATC TTACTTGAAG AAGTTGCTTC TGCCTCAACA 
CCAGCAATAA TTGAAAGTTG GCTGAGAAAA CAGATATACT CTGTGGATCA GACTAGAAAG 
AACAGTATTA GCTTCCGGGA ACTGAAGACC ATTCTTTCAC AGGCCAACTT CAAAGCTAAT 
AGCATGAGAG TGCTGAAGGA CAAGTTAACG GAGATTGGCA TTCAAAGGGA TGATATTAGT 
TTTGAACATT TTCACGTATT CTACAAAAAG CTCATGTTTG AACAGCAGAA GATGATACTG 
GATGAATTTC GGAAGGATTC TTCAGTGTTT ATTTTGGGAA ATGTTGACCG CCCAGATGGG 
TCAGCTGTCC ACCTGCATGA TTTTCAAAGG TTTCTAGTGC ATGAGCAACA GGAATCCCCA 
GCTCAAGATC TCAGTAAGGT TCGTGAACGG ATGACAAAAT TTGTAGATGA TACTATGAGG 
GAAACAGCTG AACCATTTCT TTTTGTGGAC GAGTTCTTGA CCTACTTATT TTCGAAAGAA 
AACAGCATTT GGGATGAGAA ATATGACACG ATCAGTGTTC AAAATATGAA TAATCCTCTT 
TCACATTACT GGATTGCTTC CTCACATAAT ACGTATTTAA CAGGGGACCA GCTGCGGAGT 
GAATCATCTA CTGAAGCATA TGTGCGCTGT CTGCGCATGG GATGCAGGTG CATTGAATTG 
GACTGCTGGG ATGGACCTGA TGGAAAGCCA ATTATTTATC ATGGATGGAC ACGAACAACA 
AAAATTAAAT TTGATGATGT GGTACAGGCT ATTAGGGACC ATGCATTTGC TACATCTGAG 
TATCCAGTTA TCCTTTCTAT AGAGGAACAT TGCTGTGTTG AACAACAGAG ACACATGGCC 
AAAGTTTTTA AAGAAGTATT AGGAGAGCTT CTTTTGACTA AACCAATAGA AGCTACTGCC 
GATCAGTTGC CTTCTCCCAC ACAACTTAAA GGGAGGATCA TTATTAAGCA TAAAAAACTG 
GAAACAAAGA ATCCTGGGAC AAACACTGAG AATCATAAAG AAGAACAAAA TCTTCAGGGT 
GAGCTGTACA TGTGGGATGG TATTGACCAG AAATGGTCTT CTCGTTACTG TGCTATTGCT 
GGTGATAAAT TATCTTATGG AGATGAAATT GAACAGACTG AAGACAGCGC AGCACCAAAG 
AGAACAATAG ATTTACATCT ACAAGAGAAA TGGTTCCACG GGCGAATGTA TAATGGGCGT 
TTCATGGCGG AAAAGCTACT TCAGGAGTAT TGTGCAGAAG TGGGAGGCAA AGATGGGACT 
TTCTTAGTAC GACAAAGTGA ATCTTATCGT GATGATTACA CACTCTCTTT CTGGAGGTCA 
GGCAGGGTCC AACACTGCCG TATTCGTTCC TGTACAGATG GAGAAATCAT AAAGTATTTC 
CTGACCGACA ACCTAATGTT TGATAGTATC TATGATTTGA TTCAACACTA CAAAGAGTAC 
TCATTGCGCT GTGCAGAATT TGAGCTGCGA CTAACAGATG CTGTTCCTCA ACCCAACCCT 
CACTTATCCA AAGACTGGTA CTATGATAAC CTCAGCAGAG GAGAGGCAGA AGATATGTTA 
ATGAGAATCC CTCGGGATGG GGCCTTTTTA ATAAGAAGAA GAGAGGAACA AGATTCCTAT 
GCAATTACTT TTAGAGCTGA AGGGAAAGTG AAGCACTGTA GAATACAAAG GGATGGACGC 
ATGTTTGTAC TAGGAACTTC TGCATACTTT GAAAACCTTG TGGAACTTGT CAGCTATTAC 
CAGAAACACC CGCTATACCG AAAAATGAAA CTACGCTATC CAGTTACAGA AGAGTTACTT 
CAGAGATATA GCACAGAAAA GGATTTAAGT GCCATATATG ATGTAAAGAA TAAACTGTAT 
GTCAATCCTG AAGAAATTTC TCCAGCAATG CCCCAAGGAA CAGTGAAAGC TTTGTACAAC 
TATACAGCTG TAAGATCAGA TGAGCTGAGC TTCTGCACAG GTGCCCTGAT TCATAATGTC 
TCAAGGGTCT CTGAAGGATG GTGGAAAGGA GATTATGGAG ACAAGATACA GTATTTTTTC 
CCCTCAAATT ATGTGGAGGA CATATCAATG AATGCTTCTG AAATAATTGC AAATCAGCTT 
ATTGAAGACA GTCCATTGGG GTCCATGTGC AGAGGTATCC TGGAAATGGG ATCATATAAT 
ATCAGGAAGT TGCTTAATGG AAAATATAAC AAACAGTTTG TTTTGTCCCT GGAATCCAAG 
AATCCAGAAG AGCCTACTGT AGATTTTGCA GCAGATACAA TAGAAGAGTT TTTTGTGTGG 
TACAAACACA TCCAACAGAT TGAACAGAAA AAGGGAGCAG AGGACTTTGA AAAGGGAACA 
CTGGAGAAGC AGCAAGAGAT TGCCCGAGAA TTATCTGATC TGGTTATGTA CTGTAAGCCC 
AAAAGTGAAG CTAAGGAGAA ATTAGAGTCG CCGGATTTTC GGGAAATTCG ATCATTTGCA 
GAGAATAAGG GAGAGAGTAT TGCTCGACAA AAATCCATTG AGTTGTTGCG GTATAACCGT 
AAAGCCTTTA CGCGAATCTA CCCTAAGTCC TCAAGAGTTG ACTCTTCAAA CTATGACCCC 
CTACCCCTAT GGCTCTGTGG AGACCAGATG ATTGCACTAA ATTACCAGAC TCCAGATAGG 
TACATGCAAA TAAATAATGC AATGTTCGCT TACAATGGAG AATGCGGCTA CATCCTGCAG 
CCAGAGTGTA TGAGGGAAGA GTCATATGAC CCCAGTGACT CTAGAAGACA GCTCCAAATG 
ATTGTGACAA TTAAGGTCTT AGGAGCTCGA CATTTGCCAA AACCAGGGAG AGGTATTGCA 
TGCCCTTTTG TAGAAGTGGA GGTATGCGGT GGGGAATATG ACAACAATAA GTTCAGAACA 
ATTGTTATGA ATGATAATGG CCTCAACCCT GTATGGTCAG GAAGTTCTCA ACAGGCTGAG 
TTTGAGATCC ATGAGCCTGA GATTGCCTTT CTGAGGTTTG TAGTATATGA AGAAGATATG 
TTCAGTGACC CCAACTTCTT AGCTCAGTGC ACCATCCCAG TTAAATGTTT AAAAACAGGT 
TATAGATCAG TTCCACTAAA AAACGGTTAC AGTGAAAACC TAGAGCTTGC TTCTCTGTTG 
GTTTATTGCC AAATACAAAA AGATCTGGAA AAAGTAGAAG AACTGTACTC TTCATCAAAG 
CAACTCCGCA AGCGCCAGAC AGACCTGACC AATGAACTGT TTCTTTACGA CACTCACCAA 
AACATGAGAC ACCCCAGTCA TGAGGCATTT GTGAACGACA GTAAAGGGAA TGCCAGTCCA 
GAGGTGTCCA ACAGGAGGTA TAAAATGAGA AAAGATCAGA TTGAATATAT GATCCATATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949009.1
CDS	269..4051
Translation	MDRRGSSDYRSLPEYEKSQIKRTLELGTVMTVFNYKKSQPDRRTIQVIMETGQVAWCKTNDKIEGFLDLMEIKEIRPGKNSKDFQRGKCDRCKEDVCFTIFYGNQFVLNTLSLAADSVDDAEKWKCGLTILLEEVASASTPAIIESWLRKQIYSVDQTRKNSISFRELKTILSQANFKANSMRVLKDKLTEIGIQRDDISFEHFHVFYKKLMFEQQKMILDEFRKDSSVFILGNVDRPDGSAVHLHDFQRFLVHEQQESPAQDLSKVRERMTKFVDDTMRETAEPFLFVDEFLTYLFSKENSIWDEKYDTISVQNMNNPLSHYWIASSHNTYLTGDQLRSESSTEAYVRCLRMGCRCIELDCWDGPDGKPIIYHGWTRTTKIKFDDVVQAIRDHAFATSEYPVILSIEEHCCVEQQRHMAKVFKEVLGELLLTKPIEATADQLPSPTQLKGRIIIKHKKLETKNPGTNTENHKEEQNLQGELYMWDGIDQKWSSRYCAIAGDKLSYGDEIEQTEDSAAPKRTIDLHLQEKWFHGRMYNGRFMAEKLLQEYCAEVGGKDGTFLVRQSESYRDDYTLSFWRSGRVQHCRIRSCTDGEIIKYFLTDNLMFDSIYDLIQHYKEYSLRCAEFELRLTDAVPQPNPHLSKDWYYDNLSRGEAEDMLMRIPRDGAFLIRRREEQDSYAITFRAEGKVKHCRIQRDGRMFVLGTSAYFENLVELVSYYQKHPLYRKMKLRYPVTEELLQRYSTEKDLSAIYDVKNKLYVNPEEISPAMPQGTVKALYNYTAVRSDELSFCTGALIHNVSRVSEGWWKGDYGDKIQYFFPSNYVEDISMNASEIIANQLIEDSPLGSMCRGILEMGSYNIRKLLNGKYNKQFVLSLESKNPEEPTVDFAADTIEEFFVWYKHIQQIEQKKGAEDFEKGTLEKQQEIARELSDLVMYCKPKSEAKEKLESPDFREIRSFAENKGESIARQKSIELLRYNRKAFTRIYPKSSRVDSSNYDPLPLWLCGDQMIALNYQTPDRYMQINNAMFAYNGECGYILQPECMREESYDPSDSRRQLQMIVTIKVLGARHLPKPGRGIACPFVEVEVCGGEYDNNKFRTIVMNDNGLNPVWSGSSQQAEFEIHEPEIAFLRFVVYEEDMFSDPNFLAQCTIPVKCLKTGYRSVPLKNGYSENLELASLLVYCQIQKDLEKVEELYSSSKQLRKRQTDLTNELFLYDTHQNMRHPSHEAFVNDSKGNASPEVSNRRYKMRKDQIEYMIHI

Target ORF information:

RefSeq Version	XM_018093520.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093520.2

ORF Insert Sequence:

ATGGATCGCA GGGGGAGTTC TGATTATAGA AGTCTCCCTG AATATGAGAA GAGCCAGATT 
AAGAGAACCC TTGAGCTGGG CACAGTCATG ACTGTCTTCA ACTACAAGAA GTCACAGCCT 
GATCGAAGGA CCATCCAAGT GATAATGGAA ACTGGCCAGG TGGCGTGGTG TAAGACAAAT 
GATAAGATTG AAGGATTCTT GGACCTAATG GAAATAAAGG AAATTCGTCC AGGGAAAAAT 
TCAAAGGATT TTCAACGTGG AAAATGTGAC CGTTGTAAAG AAGACGTTTG CTTCACTATT 
TTTTATGGGA ACCAATTTGT TCTGAATACT TTGAGTTTAG CAGCCGATTC AGTTGACGAT 
GCTGAAAAAT GGAAATGTGG TTTAACTATC TTACTTGAAG AAGTTGCTTC TGCCTCAACA 
CCAGCAATAA TTGAAAGTTG GCTGAGAAAA CAGATATACT CTGTGGATCA GACTAGAAAG 
AACAGTATTA GCTTCCGGGA ACTGAAGACC ATTCTTTCAC AGGCCAACTT CAAAGCTAAT 
AGCATGAGAG TGCTGAAGGA CAAGTTAACG GAGATTGGCA TTCAAAGGGA TGATATTAGT 
TTTGAACATT TTCACGTATT CTACAAAAAG CTCATGTTTG AACAGCAGAA GATGATACTG 
GATGAATTTC GGAAGGATTC TTCAGTGTTT ATTTTGGGAA ATGTTGACCG CCCAGATGGG 
TCAGCTGTCC ACCTGCATGA TTTTCAAAGG TTTCTAGTGC ATGAGCAACA GGAATCCCCA 
GCTCAAGATC TCAGTAAGGT TCGTGAACGG ATGACAAAAT TTGTAGATGA TACTATGAGG 
GAAACAGCTG AACCATTTCT TTTTGTGGAC GAGTTCTTGA CCTACTTATT TTCGAAAGAA 
AACAGCATTT GGGATGAGAA ATATGACACG ATCAGTGTTC AAAATATGAA TAATCCTCTT 
TCACATTACT GGATTGCTTC CTCACATAAT ACGTATTTAA CAGGGGACCA GCTGCGGAGT 
GAATCATCTA CTGAAGCATA TGTGCGCTGT CTGCGCATGG GATGCAGGTG CATTGAATTG 
GACTGCTGGG ATGGACCTGA TGGAAAGCCA ATTATTTATC ATGGATGGAC ACGAACAACA 
AAAATTAAAT TTGATGATGT GGTACAGGCT ATTAGGGACC ATGCATTTGC TACATCTGAG 
TATCCAGTTA TCCTTTCTAT AGAGGAACAT TGCTGTGTTG AACAACAGAG ACACATGGCC 
AAAGTTTTTA AAGAAGTATT AGGAGAGCTT CTTTTGACTA AACCAATAGA AGCTACTGCC 
GATCAGTTGC CTTCTCCCAC ACAACTTAAA GGGAGGATCA TTATTAAGCA TAAAAAACTG 
GAAACAAAGA ATCCTGGGAC AAACACTGAG AATCATAAAG AAGAACAAAA TCTTCAGGGT 
GAGCTGTACA TGTGGGATGG TATTGACCAG AAATGGTCTT CTCGTTACTG TGCTATTGCT 
GGTGATAAAT TATCTTATGG AGATGAAATT GAACAGACTG AAGACAGCGC AGCACCAAAG 
AGAACAATAG ATTTACATCT ACAAGAGAAA TGGTTCCACG GGCGAATGTA TAATGGGCGT 
TTCATGGCGG AAAAGCTACT TCAGGAGTAT TGTGCAGAAG TGGGAGGCAA AGATGGGACT 
TTCTTAGTAC GACAAAGTGA ATCTTATCGT GATGATTACA CACTCTCTTT CTGGAGGTCA 
GGCAGGGTCC AACACTGCCG TATTCGTTCC TGTACAGATG GAGAAATCAT AAAGTATTTC 
CTGACCGACA ACCTAATGTT TGATAGTATC TATGATTTGA TTCAACACTA CAAAGAGTAC 
TCATTGCGCT GTGCAGAATT TGAGCTGCGA CTAACAGATG CTGTTCCTCA ACCCAACCCT 
CACTTATCCA AAGACTGGTA CTATGATAAC CTCAGCAGAG GAGAGGCAGA AGATATGTTA 
ATGAGAATCC CTCGGGATGG GGCCTTTTTA ATAAGAAGAA GAGAGGAACA AGATTCCTAT 
GCAATTACTT TTAGAGCTGA AGGGAAAGTG AAGCACTGTA GAATACAAAG GGATGGACGC 
ATGTTTGTAC TAGGAACTTC TGCATACTTT GAAAACCTTG TGGAACTTGT CAGCTATTAC 
CAGAAACACC CGCTATACCG AAAAATGAAA CTACGCTATC CAGTTACAGA AGAGTTACTT 
CAGAGATATA GCACAGAAAA GGATTTAAGT GCCATATATG ATGTAAAGAA TAAACTGTAT 
GTCAATCCTG AAGAAATTTC TCCAGCAATG CCCCAAGGAA CAGTGAAAGC TTTGTACAAC 
TATACAGCTG TAAGATCAGA TGAGCTGAGC TTCTGCACAG GTGCCCTGAT TCATAATGTC 
TCAAGGGTCT CTGAAGGATG GTGGAAAGGA GATTATGGAG ACAAGATACA GTATTTTTTC 
CCCTCAAATT ATGTGGAGGA CATATCAATG AATGCTTCTG AAATAATTGC AAATCAGCTT 
ATTGAAGACA GTCCATTGGG GTCCATGTGC AGAGGTATCC TGGAAATGGG ATCATATAAT 
ATCAGGAAGT TGCTTAATGG AAAATATAAC AAACAGTTTG TTTTGTCCCT GGAATCCAAG 
AATCCAGAAG AGCCTACTGT AGATTTTGCA GCAGATACAA TAGAAGAGTT TTTTGTGTGG 
TACAAACACA TCCAACAGAT TGAACAGAAA AAGGGAGCAG AGGACTTTGA AAAGGGAACA 
CTGGAGAAGC AGCAAGAGAT TGCCCGAGAA TTATCTGATC TGGTTATGTA CTGTAAGCCC 
AAAAGTGAAG CTAAGGAGAA ATTAGAGTCG CCGGATTTTC GGGAAATTCG ATCATTTGCA 
GAGAATAAGG GAGAGAGTAT TGCTCGACAA AAATCCATTG AGTTGTTGCG GTATAACCGT 
AAAGCCTTTA CGCGAATCTA CCCTAAGTCC TCAAGAGTTG ACTCTTCAAA CTATGACCCC 
CTACCCCTAT GGCTCTGTGG AGACCAGATG ATTGCACTAA ATTACCAGAC TCCAGATAGG 
TACATGCAAA TAAATAATGC AATGTTCGCT TACAATGGAG AATGCGGCTA CATCCTGCAG 
CCAGAGTGTA TGAGGGAAGA GTCATATGAC CCCAGTGACT CTAGAAGACA GCTCCAAATG 
ATTGTGACAA TTAAGGTCTT AGGAGCTCGA CATTTGCCAA AACCAGGGAG AGGTATTGCA 
TGCCCTTTTG TAGAAGTGGA GGTATGCGGT GGGGAATATG ACAACAATAA GTTCAGAACA 
ATTGTTATGA ATGATAATGG CCTCAACCCT GTATGGTCAG GAAGTTCTCA ACAGGCTGAG 
TTTGAGATCC ATGAGCCTGA GATTGCCTTT CTGAGGTTTG TAGTATATGA AGAAGATATG 
TTCAGTGACC CCAACTTCTT AGCTCAGTGC ACCATCCCAG TTAAATGTTT AAAAACAGGT 
TATAGATCAG TTCCACTAAA AAACGGTTAC AGTGAAAACC TAGAGCTTGC TTCTCTGTTG 
GTTTATTGCC AAATACAAAA AGATCTGGAA AAAGTAGAAG AACTGTACTC TTCATCAAAG 
CAACTCCGCA AGCGCCAGAC AGACCTGACC AATGAACTGT TTCTTTACGA CACTCACCAA 
AACATGAGAC ACCCCAGTCA TGAGGCATTT GTGAACGACA GTAAAGGGAA TGCCAGTCCA 
GAGGTGTCCA ACAGGAGGTA TAAAATGAGA AAAGATCAGA TTGAATATAT GATCCATATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcg2 ( XM_018093522.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949011.1 Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X2, mRNA.

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CloneID	OXa36654
Clone ID Related Accession (Same CDS sequence)	XM_018093522.1 , XM_018093520.1 , XM_018093520.2 , XM_018093522.2
Accession Version	XM_018093522.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018093522.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGCA GGGGGAGTTC TGATTATAGA AGTCTCCCTG AATATGAGAA GAGCCAGATT 
AAGAGAACCC TTGAGCTGGG CACAGTCATG ACTGTCTTCA ACTACAAGAA GTCACAGCCT 
GATCGAAGGA CCATCCAAGT GATAATGGAA ACTGGCCAGG TGGCGTGGTG TAAGACAAAT 
GATAAGATTG AAGGATTCTT GGACCTAATG GAAATAAAGG AAATTCGTCC AGGGAAAAAT 
TCAAAGGATT TTCAACGTGG AAAATGTGAC CGTTGTAAAG AAGACGTTTG CTTCACTATT 
TTTTATGGGA ACCAATTTGT TCTGAATACT TTGAGTTTAG CAGCCGATTC AGTTGACGAT 
GCTGAAAAAT GGAAATGTGG TTTAACTATC TTACTTGAAG AAGTTGCTTC TGCCTCAACA 
CCAGCAATAA TTGAAAGTTG GCTGAGAAAA CAGATATACT CTGTGGATCA GACTAGAAAG 
AACAGTATTA GCTTCCGGGA ACTGAAGACC ATTCTTTCAC AGGCCAACTT CAAAGCTAAT 
AGCATGAGAG TGCTGAAGGA CAAGTTAACG GAGATTGGCA TTCAAAGGGA TGATATTAGT 
TTTGAACATT TTCACGTATT CTACAAAAAG CTCATGTTTG AACAGCAGAA GATGATACTG 
GATGAATTTC GGAAGGATTC TTCAGTGTTT ATTTTGGGAA ATGTTGACCG CCCAGATGGG 
TCAGCTGTCC ACCTGCATGA TTTTCAAAGG TTTCTAGTGC ATGAGCAACA GGAATCCCCA 
GCTCAAGATC TCAGTAAGGT TCGTGAACGG ATGACAAAAT TTGTAGATGA TACTATGAGG 
GAAACAGCTG AACCATTTCT TTTTGTGGAC GAGTTCTTGA CCTACTTATT TTCGAAAGAA 
AACAGCATTT GGGATGAGAA ATATGACACG ATCAGTGTTC AAAATATGAA TAATCCTCTT 
TCACATTACT GGATTGCTTC CTCACATAAT ACGTATTTAA CAGGGGACCA GCTGCGGAGT 
GAATCATCTA CTGAAGCATA TGTGCGCTGT CTGCGCATGG GATGCAGGTG CATTGAATTG 
GACTGCTGGG ATGGACCTGA TGGAAAGCCA ATTATTTATC ATGGATGGAC ACGAACAACA 
AAAATTAAAT TTGATGATGT GGTACAGGCT ATTAGGGACC ATGCATTTGC TACATCTGAG 
TATCCAGTTA TCCTTTCTAT AGAGGAACAT TGCTGTGTTG AACAACAGAG ACACATGGCC 
AAAGTTTTTA AAGAAGTATT AGGAGAGCTT CTTTTGACTA AACCAATAGA AGCTACTGCC 
GATCAGTTGC CTTCTCCCAC ACAACTTAAA GGGAGGATCA TTATTAAGCA TAAAAAACTG 
GAAACAAAGA ATCCTGGGAC AAACACTGAG AATCATAAAG AAGAACAAAA TCTTCAGGGT 
GAGCTGTACA TGTGGGATGG TATTGACCAG AAATGGTCTT CTCGTTACTG TGCTATTGCT 
GGTGATAAAT TATCTTATGG AGATGAAATT GAACAGACTG AAGACAGCGC AGCACCAAAG 
AGAACAATAG ATTTACATCT ACAAGAGAAA TGGTTCCACG GGCGAATGTA TAATGGGCGT 
TTCATGGCGG AAAAGCTACT TCAGGAGTAT TGTGCAGAAG TGGGAGGCAA AGATGGGACT 
TTCTTAGTAC GACAAAGTGA ATCTTATCGT GATGATTACA CACTCTCTTT CTGGAGGTCA 
GGCAGGGTCC AACACTGCCG TATTCGTTCC TGTACAGATG GAGAAATCAT AAAGTATTTC 
CTGACCGACA ACCTAATGTT TGATAGTATC TATGATTTGA TTCAACACTA CAAAGAGTAC 
TCATTGCGCT GTGCAGAATT TGAGCTGCGA CTAACAGATG CTGTTCCTCA ACCCAACCCT 
CACTTATCCA AAGACTGGTA CTATGATAAC CTCAGCAGAG GAGAGGCAGA AGATATGTTA 
ATGAGAATCC CTCGGGATGG GGCCTTTTTA ATAAGAAGAA GAGAGGAACA AGATTCCTAT 
GCAATTACTT TTAGAGCTGA AGGGAAAGTG AAGCACTGTA GAATACAAAG GGATGGACGC 
ATGTTTGTAC TAGGAACTTC TGCATACTTT GAAAACCTTG TGGAACTTGT CAGCTATTAC 
CAGAAACACC CGCTATACCG AAAAATGAAA CTACGCTATC CAGTTACAGA AGAGTTACTT 
CAGAGATATA GCACAGAAAA GGATTTAAGT GCCATATATG ATGTAAAGAA TAAACTGTAT 
GTCAATCCTG AAGAAATTTC TCCAGCAATG CCCCAAGGAA CAGTGAAAGC TTTGTACAAC 
TATACAGCTG TAAGATCAGA TGAGCTGAGC TTCTGCACAG GTGCCCTGAT TCATAATGTC 
TCAAGGGTCT CTGAAGGATG GTGGAAAGGA GATTATGGAG ACAAGATACA GTATTTTTTC 
CCCTCAAATT ATGTGGAGGA CATATCAATG AATGCTTCTG AAATAATTGC AAATCAGCTT 
ATTGAAGACA GTCCATTGGG GTCCATGTGC AGAGGTATCC TGGAAATGGG ATCATATAAT 
ATCAGGAAGT TGCTTAATGG AAAATATAAC AAACAGTTTG TTTTGTCCCT GGAATCCAAG 
AATCCAGAAG AGCCTACTGT AGATTTTGCA GCAGATACAA TAGAAGAGTT TTTTGTGTGG 
TACAAACACA TCCAACAGAT TGAACAGAAA AAGGGAGCAG AGGACTTTGA AAAGGGAACA 
CTGGAGAAGC AGCAAGAGAT TGCCCGAGAA TTATCTGATC TGGTTATGTA CTGTAAGCCC 
AAAAGTGAAG CTAAGGAGAA ATTAGAGTCG CCGGATTTTC GGGAAATTCG ATCATTTGCA 
GAGAATAAGG GAGAGAGTAT TGCTCGACAA AAATCCATTG AGTTGTTGCG GTATAACCGT 
AAAGCCTTTA CGCGAATCTA CCCTAAGTCC TCAAGAGTTG ACTCTTCAAA CTATGACCCC 
CTACCCCTAT GGCTCTGTGG AGACCAGATG ATTGCACTAA ATTACCAGAC TCCAGATAGG 
TACATGCAAA TAAATAATGC AATGTTCGCT TACAATGGAG AATGCGGCTA CATCCTGCAG 
CCAGAGTGTA TGAGGGAAGA GTCATATGAC CCCAGTGACT CTAGAAGACA GCTCCAAATG 
ATTGTGACAA TTAAGGTCTT AGGAGCTCGA CATTTGCCAA AACCAGGGAG AGGTATTGCA 
TGCCCTTTTG TAGAAGTGGA GGTATGCGGT GGGGAATATG ACAACAATAA GTTCAGAACA 
ATTGTTATGA ATGATAATGG CCTCAACCCT GTATGGTCAG GAAGTTCTCA ACAGGCTGAG 
TTTGAGATCC ATGAGCCTGA GATTGCCTTT CTGAGGTTTG TAGTATATGA AGAAGATATG 
TTCAGTGACC CCAACTTCTT AGCTCAGTGC ACCATCCCAG TTAAATGTTT AAAAACAGGT 
TATAGATCAG TTCCACTAAA AAACGGTTAC AGTGAAAACC TAGAGCTTGC TTCTCTGTTG 
GTTTATTGCC AAATACAAAA AGATCTGGAA AAAGTAGAAG AACTGTACTC TTCATCAAAG 
CAACTCCGCA AGCGCCAGAC AGACCTGACC AATGAACTGT TTCTTTACGA CACTCACCAA 
AACATGAGAC ACCCCAGTCA TGAGGCATTT GTGAACGACA GTAAAGGGAA TGCCAGTCCA 
GAGGTGTCCA ACAGGAGGTA TAAAATGAGA AAAGATCAGA TTGAATATAT GATCCATATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949011.1
CDS	137..3919
Translation	MDRRGSSDYRSLPEYEKSQIKRTLELGTVMTVFNYKKSQPDRRTIQVIMETGQVAWCKTNDKIEGFLDLMEIKEIRPGKNSKDFQRGKCDRCKEDVCFTIFYGNQFVLNTLSLAADSVDDAEKWKCGLTILLEEVASASTPAIIESWLRKQIYSVDQTRKNSISFRELKTILSQANFKANSMRVLKDKLTEIGIQRDDISFEHFHVFYKKLMFEQQKMILDEFRKDSSVFILGNVDRPDGSAVHLHDFQRFLVHEQQESPAQDLSKVRERMTKFVDDTMRETAEPFLFVDEFLTYLFSKENSIWDEKYDTISVQNMNNPLSHYWIASSHNTYLTGDQLRSESSTEAYVRCLRMGCRCIELDCWDGPDGKPIIYHGWTRTTKIKFDDVVQAIRDHAFATSEYPVILSIEEHCCVEQQRHMAKVFKEVLGELLLTKPIEATADQLPSPTQLKGRIIIKHKKLETKNPGTNTENHKEEQNLQGELYMWDGIDQKWSSRYCAIAGDKLSYGDEIEQTEDSAAPKRTIDLHLQEKWFHGRMYNGRFMAEKLLQEYCAEVGGKDGTFLVRQSESYRDDYTLSFWRSGRVQHCRIRSCTDGEIIKYFLTDNLMFDSIYDLIQHYKEYSLRCAEFELRLTDAVPQPNPHLSKDWYYDNLSRGEAEDMLMRIPRDGAFLIRRREEQDSYAITFRAEGKVKHCRIQRDGRMFVLGTSAYFENLVELVSYYQKHPLYRKMKLRYPVTEELLQRYSTEKDLSAIYDVKNKLYVNPEEISPAMPQGTVKALYNYTAVRSDELSFCTGALIHNVSRVSEGWWKGDYGDKIQYFFPSNYVEDISMNASEIIANQLIEDSPLGSMCRGILEMGSYNIRKLLNGKYNKQFVLSLESKNPEEPTVDFAADTIEEFFVWYKHIQQIEQKKGAEDFEKGTLEKQQEIARELSDLVMYCKPKSEAKEKLESPDFREIRSFAENKGESIARQKSIELLRYNRKAFTRIYPKSSRVDSSNYDPLPLWLCGDQMIALNYQTPDRYMQINNAMFAYNGECGYILQPECMREESYDPSDSRRQLQMIVTIKVLGARHLPKPGRGIACPFVEVEVCGGEYDNNKFRTIVMNDNGLNPVWSGSSQQAEFEIHEPEIAFLRFVVYEEDMFSDPNFLAQCTIPVKCLKTGYRSVPLKNGYSENLELASLLVYCQIQKDLEKVEELYSSSKQLRKRQTDLTNELFLYDTHQNMRHPSHEAFVNDSKGNASPEVSNRRYKMRKDQIEYMIHI

Target ORF information:

RefSeq Version	XM_018093522.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093522.2

ORF Insert Sequence:

ATGGATCGCA GGGGGAGTTC TGATTATAGA AGTCTCCCTG AATATGAGAA GAGCCAGATT 
AAGAGAACCC TTGAGCTGGG CACAGTCATG ACTGTCTTCA ACTACAAGAA GTCACAGCCT 
GATCGAAGGA CCATCCAAGT GATAATGGAA ACTGGCCAGG TGGCGTGGTG TAAGACAAAT 
GATAAGATTG AAGGATTCTT GGACCTAATG GAAATAAAGG AAATTCGTCC AGGGAAAAAT 
TCAAAGGATT TTCAACGTGG AAAATGTGAC CGTTGTAAAG AAGACGTTTG CTTCACTATT 
TTTTATGGGA ACCAATTTGT TCTGAATACT TTGAGTTTAG CAGCCGATTC AGTTGACGAT 
GCTGAAAAAT GGAAATGTGG TTTAACTATC TTACTTGAAG AAGTTGCTTC TGCCTCAACA 
CCAGCAATAA TTGAAAGTTG GCTGAGAAAA CAGATATACT CTGTGGATCA GACTAGAAAG 
AACAGTATTA GCTTCCGGGA ACTGAAGACC ATTCTTTCAC AGGCCAACTT CAAAGCTAAT 
AGCATGAGAG TGCTGAAGGA CAAGTTAACG GAGATTGGCA TTCAAAGGGA TGATATTAGT 
TTTGAACATT TTCACGTATT CTACAAAAAG CTCATGTTTG AACAGCAGAA GATGATACTG 
GATGAATTTC GGAAGGATTC TTCAGTGTTT ATTTTGGGAA ATGTTGACCG CCCAGATGGG 
TCAGCTGTCC ACCTGCATGA TTTTCAAAGG TTTCTAGTGC ATGAGCAACA GGAATCCCCA 
GCTCAAGATC TCAGTAAGGT TCGTGAACGG ATGACAAAAT TTGTAGATGA TACTATGAGG 
GAAACAGCTG AACCATTTCT TTTTGTGGAC GAGTTCTTGA CCTACTTATT TTCGAAAGAA 
AACAGCATTT GGGATGAGAA ATATGACACG ATCAGTGTTC AAAATATGAA TAATCCTCTT 
TCACATTACT GGATTGCTTC CTCACATAAT ACGTATTTAA CAGGGGACCA GCTGCGGAGT 
GAATCATCTA CTGAAGCATA TGTGCGCTGT CTGCGCATGG GATGCAGGTG CATTGAATTG 
GACTGCTGGG ATGGACCTGA TGGAAAGCCA ATTATTTATC ATGGATGGAC ACGAACAACA 
AAAATTAAAT TTGATGATGT GGTACAGGCT ATTAGGGACC ATGCATTTGC TACATCTGAG 
TATCCAGTTA TCCTTTCTAT AGAGGAACAT TGCTGTGTTG AACAACAGAG ACACATGGCC 
AAAGTTTTTA AAGAAGTATT AGGAGAGCTT CTTTTGACTA AACCAATAGA AGCTACTGCC 
GATCAGTTGC CTTCTCCCAC ACAACTTAAA GGGAGGATCA TTATTAAGCA TAAAAAACTG 
GAAACAAAGA ATCCTGGGAC AAACACTGAG AATCATAAAG AAGAACAAAA TCTTCAGGGT 
GAGCTGTACA TGTGGGATGG TATTGACCAG AAATGGTCTT CTCGTTACTG TGCTATTGCT 
GGTGATAAAT TATCTTATGG AGATGAAATT GAACAGACTG AAGACAGCGC AGCACCAAAG 
AGAACAATAG ATTTACATCT ACAAGAGAAA TGGTTCCACG GGCGAATGTA TAATGGGCGT 
TTCATGGCGG AAAAGCTACT TCAGGAGTAT TGTGCAGAAG TGGGAGGCAA AGATGGGACT 
TTCTTAGTAC GACAAAGTGA ATCTTATCGT GATGATTACA CACTCTCTTT CTGGAGGTCA 
GGCAGGGTCC AACACTGCCG TATTCGTTCC TGTACAGATG GAGAAATCAT AAAGTATTTC 
CTGACCGACA ACCTAATGTT TGATAGTATC TATGATTTGA TTCAACACTA CAAAGAGTAC 
TCATTGCGCT GTGCAGAATT TGAGCTGCGA CTAACAGATG CTGTTCCTCA ACCCAACCCT 
CACTTATCCA AAGACTGGTA CTATGATAAC CTCAGCAGAG GAGAGGCAGA AGATATGTTA 
ATGAGAATCC CTCGGGATGG GGCCTTTTTA ATAAGAAGAA GAGAGGAACA AGATTCCTAT 
GCAATTACTT TTAGAGCTGA AGGGAAAGTG AAGCACTGTA GAATACAAAG GGATGGACGC 
ATGTTTGTAC TAGGAACTTC TGCATACTTT GAAAACCTTG TGGAACTTGT CAGCTATTAC 
CAGAAACACC CGCTATACCG AAAAATGAAA CTACGCTATC CAGTTACAGA AGAGTTACTT 
CAGAGATATA GCACAGAAAA GGATTTAAGT GCCATATATG ATGTAAAGAA TAAACTGTAT 
GTCAATCCTG AAGAAATTTC TCCAGCAATG CCCCAAGGAA CAGTGAAAGC TTTGTACAAC 
TATACAGCTG TAAGATCAGA TGAGCTGAGC TTCTGCACAG GTGCCCTGAT TCATAATGTC 
TCAAGGGTCT CTGAAGGATG GTGGAAAGGA GATTATGGAG ACAAGATACA GTATTTTTTC 
CCCTCAAATT ATGTGGAGGA CATATCAATG AATGCTTCTG AAATAATTGC AAATCAGCTT 
ATTGAAGACA GTCCATTGGG GTCCATGTGC AGAGGTATCC TGGAAATGGG ATCATATAAT 
ATCAGGAAGT TGCTTAATGG AAAATATAAC AAACAGTTTG TTTTGTCCCT GGAATCCAAG 
AATCCAGAAG AGCCTACTGT AGATTTTGCA GCAGATACAA TAGAAGAGTT TTTTGTGTGG 
TACAAACACA TCCAACAGAT TGAACAGAAA AAGGGAGCAG AGGACTTTGA AAAGGGAACA 
CTGGAGAAGC AGCAAGAGAT TGCCCGAGAA TTATCTGATC TGGTTATGTA CTGTAAGCCC 
AAAAGTGAAG CTAAGGAGAA ATTAGAGTCG CCGGATTTTC GGGAAATTCG ATCATTTGCA 
GAGAATAAGG GAGAGAGTAT TGCTCGACAA AAATCCATTG AGTTGTTGCG GTATAACCGT 
AAAGCCTTTA CGCGAATCTA CCCTAAGTCC TCAAGAGTTG ACTCTTCAAA CTATGACCCC 
CTACCCCTAT GGCTCTGTGG AGACCAGATG ATTGCACTAA ATTACCAGAC TCCAGATAGG 
TACATGCAAA TAAATAATGC AATGTTCGCT TACAATGGAG AATGCGGCTA CATCCTGCAG 
CCAGAGTGTA TGAGGGAAGA GTCATATGAC CCCAGTGACT CTAGAAGACA GCTCCAAATG 
ATTGTGACAA TTAAGGTCTT AGGAGCTCGA CATTTGCCAA AACCAGGGAG AGGTATTGCA 
TGCCCTTTTG TAGAAGTGGA GGTATGCGGT GGGGAATATG ACAACAATAA GTTCAGAACA 
ATTGTTATGA ATGATAATGG CCTCAACCCT GTATGGTCAG GAAGTTCTCA ACAGGCTGAG 
TTTGAGATCC ATGAGCCTGA GATTGCCTTT CTGAGGTTTG TAGTATATGA AGAAGATATG 
TTCAGTGACC CCAACTTCTT AGCTCAGTGC ACCATCCCAG TTAAATGTTT AAAAACAGGT 
TATAGATCAG TTCCACTAAA AAACGGTTAC AGTGAAAACC TAGAGCTTGC TTCTCTGTTG 
GTTTATTGCC AAATACAAAA AGATCTGGAA AAAGTAGAAG AACTGTACTC TTCATCAAAG 
CAACTCCGCA AGCGCCAGAC AGACCTGACC AATGAACTGT TTCTTTACGA CACTCACCAA 
AACATGAGAC ACCCCAGTCA TGAGGCATTT GTGAACGACA GTAAAGGGAA TGCCAGTCCA 
GAGGTGTCCA ACAGGAGGTA TAAAATGAGA AAAGATCAGA TTGAATATAT GATCCATATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcg2 ( XM_012962308.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817762.1 Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X4, mRNA.

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CloneID	OXa36655
Clone ID Related Accession (Same CDS sequence)	XM_012962308.2 , XM_012962308.3
Accession Version	XM_012962308.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962308.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGCA GGGGGAGTTC TGATTATAGA AGTCTCCCTG AATATGAGAA GAGCCAGATT 
AAGAGAACCC TTGAGCTGGG CACAGTCATG ACTGTCTTCA ACTACAAGAA GTCACAGCCT 
GATCGAAGGA CCATCCAAGT GATAATGGAA ACTGGCCAGG TGGCGTGGTG TAAGACAAAT 
GATAAGATTG AAGGATTCTT GGACCTAATG GAAATAAAGG AAATTCGTCC AGGGAAAAAT 
TCAAAGGATT TTCAACGTGG AAAATGTGAC CGTTGTAAAG AAGACGTTTG CTTCACTATT 
TTTTATGGGA ACCAATTTGT TCTGAATACT TTGAGTTTAG CAGCCGATTC AGTTGACGAT 
GCTGAAAAAT GGAAATGTGG TTTAACTATC TTACTTGAAG AAGTTGCTTC TGCCTCAACA 
CCAGCAATAA TTGAAAGTTG GCTGAGAAAA CAGATATACT CTGTGGATCA GACTAGAAAG 
AACAGTATTA GCTTCCGGGA ACTGAAGACC ATTCTTTCAC AGGCCAACTT CAAAGCTAAT 
AGCATGAGAG TGCTGAAGGA CAAGTTAACG GAGATTGGCA TTCAAAGGGA TGATATTAGT 
TTTGAACATT TTCACGTATT CTACAAAAAG CTCATGTTTG AACAGCAGAA GATGATACTG 
GATGAATTTC GGAAGGATTC TTCAGTGTTT ATTTTGGGAA ATGTTGACCG CCCAGATGGG 
TCAGCTGTCC ACCTGCATGA TTTTCAAAGG TTTCTAGTGC ATGAGCAACA GGAATCCCCA 
GCTCAAGATC TCAGTAAGGT TCGTGAACGG ATGACAAAAT TTGTAGATGA TACTATGAGG 
GAAACAGCTG AACCATTTCT TTTTGTGGAC GAGTTCTTGA CCTACTTATT TTCGAAAGAA 
AACAGCATTT GGGATGAGAA ATATGACACG ATCAGTGTTC AAAATATGAA TAATCCTCTT 
TCACATTACT GGATTGCTTC CTCACATAAT ACGTATTTAA CAGGGGACCA GCTGCGGAGT 
GAATCATCTA CTGAAGCATA TGTGCGCTGT CTGCGCATGG GATGCAGGTG CATTGAATTG 
GACTGCTGGG ATGGACCTGA TGGAAAGCCA ATTATTTATC ATGGATGGAC ACGAACAACA 
AAAATTAAAT TTGATGATGT GGTACAGGCT ATTAGGGACC ATGCATTTGC TACATCTGAG 
TATCCAGTTA TCCTTTCTAT AGAGGAACAT TGCTGTGTTG AACAACAGAG ACACATGGCC 
AAAGTTTTTA AAGAAGTATT AGGAGAGCTT CTTTTGACTA AACCAATAGA AGCTACTGCC 
GATCAGTTGC CTTCTCCCAC ACAACTTAAA GGGAGGATCA TTATTAAGCA TAAAAAACTG 
GAAACAAAGA ATCCTGGGAC AAACACTGAG AATCATAAAG AAGAACAAAA TCTTCAGGGT 
GAGCTGTACA TGTGGGATGG TATTGACCAG AAATGGTCTT CTCGTTACTG TGCTATTGCT 
GGTGATAAAT TATCTTATGG AGATGAAATT GAACAGACTG AAGACAGCGC AGCACCAAAG 
AGAACAATAG ATTTACATCT ACAAGAGAAA TGGTTCCACG GGCGAATGTA TAATGGGCGT 
TTCATGGCGG AAAAGCTACT TCAGGAGTAT TGTGCAGAAG TGGGAGGCAA AGATGGGACT 
TTCTTAGTAC GACAAAGTGA ATCTTATCGT GATGATTACA CACTCTCTTT CTGGAGGTCA 
GGCAGGGTCC AACACTGCCG TATTCGTTCC TGTACAGATG GAGAAATCAT AAAGTATTTC 
CTGACCGACA ACCTAATGTT TGATAGTATC TATGATTTGA TTCAACACTA CAAAGAGTAC 
TCATTGCGCT GTGCAGAATT TGAGCTGCGA CTAACAGATG CTGTTCCTCA ACCCAACCCT 
CACTTATCCA AAGACTGGTA CTATGATAAC CTCAGCAGAG GAGAGGCAGA AGATATGTTA 
ATGAGAATCC CTCGGGATGG GGCCTTTTTA ATAAGAAGAA GAGAGGAACA AGATTCCTAT 
GCAATTACTT TTAGAGCTGA AGGGAAAGTG AAGCACTGTA GAATACAAAG GGATGGACGC 
ATGTTTGTAC TAGGAACTTC TGCATACTTT GAAAACCTTG TGGAACTTGT CAGCTATTAC 
CAGAAACACC CGCTATACCG AAAAATGAAA CTACGCTATC CAGTTACAGA AGAGTTACTT 
CAGAGATATA GCACAGAAAA GGATTTAAGT GCCATATATG ATGTAAAGAA TAAACTGTAT 
GTCAATCCTG AAGAAATTTC TCCAGCAATG CCCCAAGGAA CAGTGAAAGC TTTGTACAAC 
TATACAGCTG TAAGATCAGA TGAGCTGAGC TTCTGCACAG GTGCCCTGAT TCATAATGTC 
TCAAGGGTCT CTGAAGGATG GTGGAAAGGA GATTATGGAG ACAAGATACA GTATTTTTTC 
CCCTCAAATT ATGTGGAGGA CATATCAATG AATGCTTCTG AAATAATTGC AAATCAGCTT 
ATTGAAGACA GTCCATTGGG GTCCATGTGC AGAGGTATCC TGGAAATGGG ATCATATAAT 
ATCAGGAAGT TGCTTAATGG AAAATATAAC AAACAGTTTG TTTTGTCCCT GGAATCCAAG 
AATCCAGAAG AGCCTACTGT AGATTTTGCA GCAGATACAA TAGAAGAGTT TTTTGTGTGG 
TACAAACACA TCCAACAGAT TGAACAGAAA AAGGGAGCAG AGGACTTTGA AAAGGGAACA 
CTGGAGAAGC AGCAAGAGAT TGCCCGAGAA TTATCTGATC TGGTTATGTA CTGTAAGCCC 
AAAAGTGAAG CTAAGGAGAA ATTAGAGTCG CCGGATTTTC GGGAAATTCG ATCATTTGCA 
GAGAATAAGG GAGAGAGTAT TGCTCGACAA AAATCCATTG AGTTGTTGCG GTATAACCGT 
AAAGCCTTTA CGCGAATCTA CCCTAAGTCC TCAAGAGTTG ACTCTTCAAA CTATGACCCC 
CTACCCCTAT GGCTCTGTGG AGACCAGATG ATTGCACTAA ATTACCAGAC TCCAGATAGG 
TACATGCAAA TAAATAATGC AATGTTCGCT TACAATGGAG AATGCGGCTA CATCCTGCAG 
CCAGAGTGTA TGAGGGAAGA GTCATATGAC CCCAGTGACT CTAGAAGACA GCTCCAAATG 
ATTGTGACAA TTAAGGTCTT AGGAGCTCGA CATTTGCCAA AACCAGGGAG AGGTATTGCA 
TGCCCTTTTG TAGAAGTGGA GGTATGCGGT GGGGAATATG ACAACAATAA GTTCAGAACA 
ATTGTTATGA ATGATAATGG CCTCAACCCT GTATGGTCAG GAAGTTCTCA ACAGGCTGAG 
TTTGAGATCC ATGAGCCTGA GATTGCCTTT CTGAGGTTTG TAGTATATGA AGAAGATATG 
TTCAGTGACC CCAACTTCTT AGCTCAGTGC ACCATCCCAG TTAAATGTTT AAAAACAGGT 
TATAGATCAG TTCCACTAAA AAACGGTTAC AGTGAAAACC TAGAGCTTGC TTCTCTGTTG 
GTTTATTGCC AAATACAAAA AGATCTGACT CGCCCACGTG CAAAATTCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817762.1
CDS	269..3862
Translation	MDRRGSSDYRSLPEYEKSQIKRTLELGTVMTVFNYKKSQPDRRTIQVIMETGQVAWCKTNDKIEGFLDLMEIKEIRPGKNSKDFQRGKCDRCKEDVCFTIFYGNQFVLNTLSLAADSVDDAEKWKCGLTILLEEVASASTPAIIESWLRKQIYSVDQTRKNSISFRELKTILSQANFKANSMRVLKDKLTEIGIQRDDISFEHFHVFYKKLMFEQQKMILDEFRKDSSVFILGNVDRPDGSAVHLHDFQRFLVHEQQESPAQDLSKVRERMTKFVDDTMRETAEPFLFVDEFLTYLFSKENSIWDEKYDTISVQNMNNPLSHYWIASSHNTYLTGDQLRSESSTEAYVRCLRMGCRCIELDCWDGPDGKPIIYHGWTRTTKIKFDDVVQAIRDHAFATSEYPVILSIEEHCCVEQQRHMAKVFKEVLGELLLTKPIEATADQLPSPTQLKGRIIIKHKKLETKNPGTNTENHKEEQNLQGELYMWDGIDQKWSSRYCAIAGDKLSYGDEIEQTEDSAAPKRTIDLHLQEKWFHGRMYNGRFMAEKLLQEYCAEVGGKDGTFLVRQSESYRDDYTLSFWRSGRVQHCRIRSCTDGEIIKYFLTDNLMFDSIYDLIQHYKEYSLRCAEFELRLTDAVPQPNPHLSKDWYYDNLSRGEAEDMLMRIPRDGAFLIRRREEQDSYAITFRAEGKVKHCRIQRDGRMFVLGTSAYFENLVELVSYYQKHPLYRKMKLRYPVTEELLQRYSTEKDLSAIYDVKNKLYVNPEEISPAMPQGTVKALYNYTAVRSDELSFCTGALIHNVSRVSEGWWKGDYGDKIQYFFPSNYVEDISMNASEIIANQLIEDSPLGSMCRGILEMGSYNIRKLLNGKYNKQFVLSLESKNPEEPTVDFAADTIEEFFVWYKHIQQIEQKKGAEDFEKGTLEKQQEIARELSDLVMYCKPKSEAKEKLESPDFREIRSFAENKGESIARQKSIELLRYNRKAFTRIYPKSSRVDSSNYDPLPLWLCGDQMIALNYQTPDRYMQINNAMFAYNGECGYILQPECMREESYDPSDSRRQLQMIVTIKVLGARHLPKPGRGIACPFVEVEVCGGEYDNNKFRTIVMNDNGLNPVWSGSSQQAEFEIHEPEIAFLRFVVYEEDMFSDPNFLAQCTIPVKCLKTGYRSVPLKNGYSENLELASLLVYCQIQKDLTRPRAKFR

Target ORF information:

RefSeq Version	XM_012962308.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962308.3

ORF Insert Sequence:

ATGGATCGCA GGGGGAGTTC TGATTATAGA AGTCTCCCTG AATATGAGAA GAGCCAGATT 
AAGAGAACCC TTGAGCTGGG CACAGTCATG ACTGTCTTCA ACTACAAGAA GTCACAGCCT 
GATCGAAGGA CCATCCAAGT GATAATGGAA ACTGGCCAGG TGGCGTGGTG TAAGACAAAT 
GATAAGATTG AAGGATTCTT GGACCTAATG GAAATAAAGG AAATTCGTCC AGGGAAAAAT 
TCAAAGGATT TTCAACGTGG AAAATGTGAC CGTTGTAAAG AAGACGTTTG CTTCACTATT 
TTTTATGGGA ACCAATTTGT TCTGAATACT TTGAGTTTAG CAGCCGATTC AGTTGACGAT 
GCTGAAAAAT GGAAATGTGG TTTAACTATC TTACTTGAAG AAGTTGCTTC TGCCTCAACA 
CCAGCAATAA TTGAAAGTTG GCTGAGAAAA CAGATATACT CTGTGGATCA GACTAGAAAG 
AACAGTATTA GCTTCCGGGA ACTGAAGACC ATTCTTTCAC AGGCCAACTT CAAAGCTAAT 
AGCATGAGAG TGCTGAAGGA CAAGTTAACG GAGATTGGCA TTCAAAGGGA TGATATTAGT 
TTTGAACATT TTCACGTATT CTACAAAAAG CTCATGTTTG AACAGCAGAA GATGATACTG 
GATGAATTTC GGAAGGATTC TTCAGTGTTT ATTTTGGGAA ATGTTGACCG CCCAGATGGG 
TCAGCTGTCC ACCTGCATGA TTTTCAAAGG TTTCTAGTGC ATGAGCAACA GGAATCCCCA 
GCTCAAGATC TCAGTAAGGT TCGTGAACGG ATGACAAAAT TTGTAGATGA TACTATGAGG 
GAAACAGCTG AACCATTTCT TTTTGTGGAC GAGTTCTTGA CCTACTTATT TTCGAAAGAA 
AACAGCATTT GGGATGAGAA ATATGACACG ATCAGTGTTC AAAATATGAA TAATCCTCTT 
TCACATTACT GGATTGCTTC CTCACATAAT ACGTATTTAA CAGGGGACCA GCTGCGGAGT 
GAATCATCTA CTGAAGCATA TGTGCGCTGT CTGCGCATGG GATGCAGGTG CATTGAATTG 
GACTGCTGGG ATGGACCTGA TGGAAAGCCA ATTATTTATC ATGGATGGAC ACGAACAACA 
AAAATTAAAT TTGATGATGT GGTACAGGCT ATTAGGGACC ATGCATTTGC TACATCTGAG 
TATCCAGTTA TCCTTTCTAT AGAGGAACAT TGCTGTGTTG AACAACAGAG ACACATGGCC 
AAAGTTTTTA AAGAAGTATT AGGAGAGCTT CTTTTGACTA AACCAATAGA AGCTACTGCC 
GATCAGTTGC CTTCTCCCAC ACAACTTAAA GGGAGGATCA TTATTAAGCA TAAAAAACTG 
GAAACAAAGA ATCCTGGGAC AAACACTGAG AATCATAAAG AAGAACAAAA TCTTCAGGGT 
GAGCTGTACA TGTGGGATGG TATTGACCAG AAATGGTCTT CTCGTTACTG TGCTATTGCT 
GGTGATAAAT TATCTTATGG AGATGAAATT GAACAGACTG AAGACAGCGC AGCACCAAAG 
AGAACAATAG ATTTACATCT ACAAGAGAAA TGGTTCCACG GGCGAATGTA TAATGGGCGT 
TTCATGGCGG AAAAGCTACT TCAGGAGTAT TGTGCAGAAG TGGGAGGCAA AGATGGGACT 
TTCTTAGTAC GACAAAGTGA ATCTTATCGT GATGATTACA CACTCTCTTT CTGGAGGTCA 
GGCAGGGTCC AACACTGCCG TATTCGTTCC TGTACAGATG GAGAAATCAT AAAGTATTTC 
CTGACCGACA ACCTAATGTT TGATAGTATC TATGATTTGA TTCAACACTA CAAAGAGTAC 
TCATTGCGCT GTGCAGAATT TGAGCTGCGA CTAACAGATG CTGTTCCTCA ACCCAACCCT 
CACTTATCCA AAGACTGGTA CTATGATAAC CTCAGCAGAG GAGAGGCAGA AGATATGTTA 
ATGAGAATCC CTCGGGATGG GGCCTTTTTA ATAAGAAGAA GAGAGGAACA AGATTCCTAT 
GCAATTACTT TTAGAGCTGA AGGGAAAGTG AAGCACTGTA GAATACAAAG GGATGGACGC 
ATGTTTGTAC TAGGAACTTC TGCATACTTT GAAAACCTTG TGGAACTTGT CAGCTATTAC 
CAGAAACACC CGCTATACCG AAAAATGAAA CTACGCTATC CAGTTACAGA AGAGTTACTT 
CAGAGATATA GCACAGAAAA GGATTTAAGT GCCATATATG ATGTAAAGAA TAAACTGTAT 
GTCAATCCTG AAGAAATTTC TCCAGCAATG CCCCAAGGAA CAGTGAAAGC TTTGTACAAC 
TATACAGCTG TAAGATCAGA TGAGCTGAGC TTCTGCACAG GTGCCCTGAT TCATAATGTC 
TCAAGGGTCT CTGAAGGATG GTGGAAAGGA GATTATGGAG ACAAGATACA GTATTTTTTC 
CCCTCAAATT ATGTGGAGGA CATATCAATG AATGCTTCTG AAATAATTGC AAATCAGCTT 
ATTGAAGACA GTCCATTGGG GTCCATGTGC AGAGGTATCC TGGAAATGGG ATCATATAAT 
ATCAGGAAGT TGCTTAATGG AAAATATAAC AAACAGTTTG TTTTGTCCCT GGAATCCAAG 
AATCCAGAAG AGCCTACTGT AGATTTTGCA GCAGATACAA TAGAAGAGTT TTTTGTGTGG 
TACAAACACA TCCAACAGAT TGAACAGAAA AAGGGAGCAG AGGACTTTGA AAAGGGAACA 
CTGGAGAAGC AGCAAGAGAT TGCCCGAGAA TTATCTGATC TGGTTATGTA CTGTAAGCCC 
AAAAGTGAAG CTAAGGAGAA ATTAGAGTCG CCGGATTTTC GGGAAATTCG ATCATTTGCA 
GAGAATAAGG GAGAGAGTAT TGCTCGACAA AAATCCATTG AGTTGTTGCG GTATAACCGT 
AAAGCCTTTA CGCGAATCTA CCCTAAGTCC TCAAGAGTTG ACTCTTCAAA CTATGACCCC 
CTACCCCTAT GGCTCTGTGG AGACCAGATG ATTGCACTAA ATTACCAGAC TCCAGATAGG 
TACATGCAAA TAAATAATGC AATGTTCGCT TACAATGGAG AATGCGGCTA CATCCTGCAG 
CCAGAGTGTA TGAGGGAAGA GTCATATGAC CCCAGTGACT CTAGAAGACA GCTCCAAATG 
ATTGTGACAA TTAAGGTCTT AGGAGCTCGA CATTTGCCAA AACCAGGGAG AGGTATTGCA 
TGCCCTTTTG TAGAAGTGGA GGTATGCGGT GGGGAATATG ACAACAATAA GTTCAGAACA 
ATTGTTATGA ATGATAATGG CCTCAACCCT GTATGGTCAG GAAGTTCTCA ACAGGCTGAG 
TTTGAGATCC ATGAGCCTGA GATTGCCTTT CTGAGGTTTG TAGTATATGA AGAAGATATG 
TTCAGTGACC CCAACTTCTT AGCTCAGTGC ACCATCCCAG TTAAATGTTT AAAAACAGGT 
TATAGATCAG TTCCACTAAA AAACGGTTAC AGTGAAAACC TAGAGCTTGC TTCTCTGTTG 
GTTTATTGCC AAATACAAAA AGATCTGACT CGCCCACGTG CAAAATTCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcg2 ( XM_002934948.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934994.2 Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa08886
Clone ID Related Accession (Same CDS sequence)	XM_002934948.4 , XM_002934948.5
Accession Version	XM_002934948.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934948.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGCA GGGGGAGTTC TGATTATAGA AGTCTCCCTG AATATGAGAA GAGCCAGATT 
AAGAGAACCC TTGAGCTGGG CACAGTCATG ACTGTCTTCA ACTACAAGAA GTCACAGCCT 
GATCGAAGGA CCATCCAAGT GATAATGGAA ACTGGCCAGG TGGCGTGGTG TAAGACAAAT 
GATAAGATTG AAGGATTCTT GGACCTAATG GAAATAAAGG AAATTCGTCC AGGGAAAAAT 
TCAAAGGATT TTCAACGTGG AAAATGTGAC CGTTGTAAAG AAGACGTTTG CTTCACTATT 
TTTTATGGGA ACCAATTTGT TCTGAATACT TTGAGTTTAG CAGCCGATTC AGTTGACGAT 
GCTGAAAAAT GGAAATGTGG TTTAACTATC TTACTTGAAG AAGTTGCTTC TGCCTCAACA 
CCAGCAATAA TTGAAAGTTG GCTGAGAAAA CAGATATACT CTGTGGATCA GACTAGAAAG 
AACAGTATTA GCTTCCGGGA ACTGAAGACC ATTCTTTCAC AGGCCAACTT CAAAGCTAAT 
AGCATGAGAG TGCTGAAGGA CAAGTTAACG GAGATTGGCA TTCAAAGGGA TGATATTAGT 
TTTGAACATT TTCACGTATT CTACAAAAAG CTCATGTTTG AACAGCAGAA GATGATACTG 
GATGAATTTC GGAAGGATTC TTCAGTGTTT ATTTTGGGAA ATGTTGACCG CCCAGATGGG 
TCAGCTGTCC ACCTGCATGA TTTTCAAAGG TTTCTAGTGC ATGAGCAACA GGAATCCCCA 
GCTCAAGATC TCAGTAAGGT TCGTGAACGG ATGACAAAAT TTGTAGATGA TACTATGAGG 
GAAACAGCTG AACCATTTCT TTTTGTGGAC GAGTTCTTGA CCTACTTATT TTCGAAAGAA 
AACAGCATTT GGGATGAGAA ATATGACACG ATCAGTGTTC AAAATATGAA TAATCCTCTT 
TCACATTACT GGATTGCTTC CTCACATAAT ACGTATTTAA CAGGGGACCA GCTGCGGAGT 
GAATCATCTA CTGAAGCATA TGTGCGCTGT CTGCGCATGG GATGCAGGTG CATTGAATTG 
GACTGCTGGG ATGGACCTGA TGGAAAGCCA ATTATTTATC ATGGATGGAC ACGAACAACA 
AAAATTAAAT TTGATGATGT GGTACAGGCT ATTAGGGACC ATGCATTTGC TACATCTGAG 
TATCCAGTTA TCCTTTCTAT AGAGGAACAT TGCTGTGTTG AACAACAGAG ACACATGGCC 
AAAGTTTTTA AAGAAGTATT AGGAGAGCTT CTTTTGACTA AACCAATAGA AGCTACTGCC 
GATCAGTTGC CTTCTCCCAC ACAACTTAAA GGGAGGATCA TTATTAAGCA TAAAAAACTG 
GAAACAAAGA ATCCTGGGAC AAACACTGAG AATCATAAAG AAGAACAAAA TCTTCAGGGT 
GAGCTGTACA TGTGGGATGG TATTGACCAG AAATGGTCTT CTCGTTACTG TGCTATTGCT 
GGTGATAAAT TATCTTATGG AGATGAAATT GAACAGACTG AAGACAGCGC AGCACCAAAG 
AGAACAATAG ATTTACATCT ACAAGAGAAA TGGTTCCACG GGCGAATGTA TAATGGGCGT 
TTCATGGCGG AAAAGCTACT TCAGGAGTAT TGTGCAGAAG TGGGAGGCAA AGATGGGACT 
TTCTTAGTAC GACAAAGTGA ATCTTATCGT GATGATTACA CACTCTCTTT CTGGAGGTCA 
GGCAGGGTCC AACACTGCCG TATTCGTTCC TGTACAGATG GAGAAATCAT AAAGTATTTC 
CTGACCGACA ACCTAATGTT TGATAGTATC TATGATTTGA TTCAACACTA CAAAGAGTAC 
TCATTGCGCT GTGCAGAATT TGAGCTGCGA CTAACAGATG CTGTTCCTCA ACCCAACCCT 
CACTTATCCA AAGACTGGTA CTATGATAAC CTCAGCAGAG GAGAGGCAGA AGATATGTTA 
ATGAGAATCC CTCGGGATGG GGCCTTTTTA ATAAGAAGAA GAGAGGAACA AGATTCCTAT 
GCAATTACTT TTAGAGCTGA AGGGAAAGTG AAGCACTGTA GAATACAAAG GGATGGACGC 
ATGTTTGTAC TAGGAACTTC TGCATACTTT GAAAACCTTG TGGAACTTGT CAGCTATTAC 
CAGAAACACC CGCTATACCG AAAAATGAAA CTACGCTATC CAGTTACAGA AGAGTTACTT 
CAGAGATATA GCACAGAAAA GGATTTAAGT GCCATATATG ATGTAAAGAA TAAACTGTAT 
GTCAATCCTG AAGAAATTTC TCCAGCAATG CCCCAAGGAA CAGTGAAAGC TTTGTACAAC 
TATACAGCTG TAAGATCAGA TGAGCTGAGC TTCTGCACAG GTGCCCTGAT TCATAATGTC 
TCAAGGGTCT CTGAAGGATG GTGGAAAGGA GATTATGGAG ACAAGATACA GTATTTTTTC 
CCCTCAAATT ATGTGGAGGA CATATCAATG AATGCTTCTG AAATAATTGC AAATCAGCTT 
ATTGAAGACA GTCCATTGGG GTCCATGTGC AGAGGTATCC TGGAAATGGG ATCATATAAT 
ATCAGGAAGT TGCTTAATGG AAAATATAAC AAACAGTTTG TTTTGTCCCT GGAATCCAAG 
AATCCAGAAG AGCCTACTGT AGATTTTGCA GCAGATACAA TAGAAGAGTT TTTTGTGTGG 
TACAAACACA TCCAACAGAT TGAACAGAAA AAGGGAGCAG AGGACTTTGA AAAGGGAACA 
CTGGAGAAGC AGCAAGAGAT TGCCCGAGAA TTATCTGATC TGGTTATGTA CTGTAAGCCC 
AAAAGTGAAG CTAAGGAGAA ATTAGAGTCG CCGGATTTTC GGGAAATTCG ATCATTTGCA 
GAGAATAAGG GAGAGAGTAT TGCTCGACAA AAATCCATTG AGTTGTTGCG GTATAACCGT 
AAAGCCTTTA CGCGAATCTA CCCTAAGTCC TCAAGAGTTG ACTCTTCAAA CTATGACCCC 
CTACCCCTAT GGCTCTGTGG AGACCAGATG ATTGCACTAA ATTACCAGAC TCCAGATAGG 
TACATGCAAA TAAATAATGC AATGTTCGCT TACAATGGAG AATGCGGCTA CATCCTGCAG 
CCAGAGTGTA TGAGGGAAGA GTCATATGAC CCCAGTGACT CTAGAAGACA GCTCCAAATG 
ATTGTGACAA TTAAGGTCTT AGGAGCTCGA CATTTGCCAA AACCAGGGAG AGGTATTGCA 
TGCCCTTTTG TAGAAGTGGA GGTATGCGGT GGGGAATATG ACAACAATAA GTTCAGAACA 
ATTGTTATGA ATGATAATGG CCTCAACCCT GTATGGTCAG GAAGTTCTCA ACAGGCTGAG 
TTTGAGATCC ATGAGCCTGA GATTGCCTTT CTGAGGTTTG TAGTATATGA AGAAGATATG 
TTCAGTGACC CCAACTTCTT AGCTCAGTGC ACCATCCCAG TTAAATGTTT AAAAACAGGT 
TATAGATCAG TTCCACTAAA AAACGGTTAC AGTGAAAACC TAGAGCTTGC TTCTCTGTTG 
GTTTATTGCC AAATACAAAA AGATCTGGAA AAAGTAGAAG AACTGTACTC TTCATCAAAG 
CAACTCCGCA AGCGCCAGAC AGACCTGACC AATGAACTGT TTCTTTACGA CACTCACCAA 
AACATGAGAC ACCCCAGTCA TGAGGCATTT GTGAACGACA GTAAAGGGAA TGCCAGTCCA 
GAGGTGTCCA ACAGGAGGTT AAAGGAAAGA CAGATCAGTA ACAGCAAATT CTACTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934994.2
CDS	268..4047
Translation	MDRRGSSDYRSLPEYEKSQIKRTLELGTVMTVFNYKKSQPDRRTIQVIMETGQVAWCKTNDKIEGFLDLMEIKEIRPGKNSKDFQRGKCDRCKEDVCFTIFYGNQFVLNTLSLAADSVDDAEKWKCGLTILLEEVASASTPAIIESWLRKQIYSVDQTRKNSISFRELKTILSQANFKANSMRVLKDKLTEIGIQRDDISFEHFHVFYKKLMFEQQKMILDEFRKDSSVFILGNVDRPDGSAVHLHDFQRFLVHEQQESPAQDLSKVRERMTKFVDDTMRETAEPFLFVDEFLTYLFSKENSIWDEKYDTISVQNMNNPLSHYWIASSHNTYLTGDQLRSESSTEAYVRCLRMGCRCIELDCWDGPDGKPIIYHGWTRTTKIKFDDVVQAIRDHAFATSEYPVILSIEEHCCVEQQRHMAKVFKEVLGELLLTKPIEATADQLPSPTQLKGRIIIKHKKLETKNPGTNTENHKEEQNLQGELYMWDGIDQKWSSRYCAIAGDKLSYGDEIEQTEDSAAPKRTIDLHLQEKWFHGRMYNGRFMAEKLLQEYCAEVGGKDGTFLVRQSESYRDDYTLSFWRSGRVQHCRIRSCTDGEIIKYFLTDNLMFDSIYDLIQHYKEYSLRCAEFELRLTDAVPQPNPHLSKDWYYDNLSRGEAEDMLMRIPRDGAFLIRRREEQDSYAITFRAEGKVKHCRIQRDGRMFVLGTSAYFENLVELVSYYQKHPLYRKMKLRYPVTEELLQRYSTEKDLSAIYDVKNKLYVNPEEISPAMPQGTVKALYNYTAVRSDELSFCTGALIHNVSRVSEGWWKGDYGDKIQYFFPSNYVEDISMNASEIIANQLIEDSPLGSMCRGILEMGSYNIRKLLNGKYNKQFVLSLESKNPEEPTVDFAADTIEEFFVWYKHIQQIEQKKGAEDFEKGTLEKQQEIARELSDLVMYCKPKSEAKEKLESPDFREIRSFAENKGESIARQKSIELLRYNRKAFTRIYPKSSRVDSSNYDPLPLWLCGDQMIALNYQTPDRYMQINNAMFAYNGECGYILQPECMREESYDPSDSRRQLQMIVTIKVLGARHLPKPGRGIACPFVEVEVCGGEYDNNKFRTIVMNDNGLNPVWSGSSQQAEFEIHEPEIAFLRFVVYEEDMFSDPNFLAQCTIPVKCLKTGYRSVPLKNGYSENLELASLLVYCQIQKDLEKVEELYSSSKQLRKRQTDLTNELFLYDTHQNMRHPSHEAFVNDSKGNASPEVSNRRLKERQISNSKFYS

Target ORF information:

RefSeq Version	XM_002934948.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934948.4

ORF Insert Sequence:

ATGGATCGCA GGGGGAGTTC TGATTATAGA AGTCTCCCTG AATATGAGAA GAGCCAGATT 
AAGAGAACCC TTGAGCTGGG CACAGTCATG ACTGTCTTCA ACTACAAGAA GTCACAGCCT 
GATCGAAGGA CCATCCAAGT GATAATGGAA ACTGGCCAGG TGGCGTGGTG TAAGACAAAT 
GATAAGATTG AAGGATTCTT GGACCTAATG GAAATAAAGG AAATTCGTCC AGGGAAAAAT 
TCAAAGGATT TTCAACGTGG AAAATGTGAC CGTTGTAAAG AAGACGTTTG CTTCACTATT 
TTTTATGGGA ACCAATTTGT TCTGAATACT TTGAGTTTAG CAGCCGATTC AGTTGACGAT 
GCTGAAAAAT GGAAATGTGG TTTAACTATC TTACTTGAAG AAGTTGCTTC TGCCTCAACA 
CCAGCAATAA TTGAAAGTTG GCTGAGAAAA CAGATATACT CTGTGGATCA GACTAGAAAG 
AACAGTATTA GCTTCCGGGA ACTGAAGACC ATTCTTTCAC AGGCCAACTT CAAAGCTAAT 
AGCATGAGAG TGCTGAAGGA CAAGTTAACG GAGATTGGCA TTCAAAGGGA TGATATTAGT 
TTTGAACATT TTCACGTATT CTACAAAAAG CTCATGTTTG AACAGCAGAA GATGATACTG 
GATGAATTTC GGAAGGATTC TTCAGTGTTT ATTTTGGGAA ATGTTGACCG CCCAGATGGG 
TCAGCTGTCC ACCTGCATGA TTTTCAAAGG TTTCTAGTGC ATGAGCAACA GGAATCCCCA 
GCTCAAGATC TCAGTAAGGT TCGTGAACGG ATGACAAAAT TTGTAGATGA TACTATGAGG 
GAAACAGCTG AACCATTTCT TTTTGTGGAC GAGTTCTTGA CCTACTTATT TTCGAAAGAA 
AACAGCATTT GGGATGAGAA ATATGACACG ATCAGTGTTC AAAATATGAA TAATCCTCTT 
TCACATTACT GGATTGCTTC CTCACATAAT ACGTATTTAA CAGGGGACCA GCTGCGGAGT 
GAATCATCTA CTGAAGCATA TGTGCGCTGT CTGCGCATGG GATGCAGGTG CATTGAATTG 
GACTGCTGGG ATGGACCTGA TGGAAAGCCA ATTATTTATC ATGGATGGAC ACGAACAACA 
AAAATTAAAT TTGATGATGT GGTACAGGCT ATTAGGGACC ATGCATTTGC TACATCTGAG 
TATCCAGTTA TCCTTTCTAT AGAGGAACAT TGCTGTGTTG AACAACAGAG ACACATGGCC 
AAAGTTTTTA AAGAAGTATT AGGAGAGCTT CTTTTGACTA AACCAATAGA AGCTACTGCC 
GATCAGTTGC CTTCTCCCAC ACAACTTAAA GGGAGGATCA TTATTAAGCA TAAAAAACTG 
GAAACAAAGA ATCCTGGGAC AAACACTGAG AATCATAAAG AAGAACAAAA TCTTCAGGGT 
GAGCTGTACA TGTGGGATGG TATTGACCAG AAATGGTCTT CTCGTTACTG TGCTATTGCT 
GGTGATAAAT TATCTTATGG AGATGAAATT GAACAGACTG AAGACAGCGC AGCACCAAAG 
AGAACAATAG ATTTACATCT ACAAGAGAAA TGGTTCCACG GGCGAATGTA TAATGGGCGT 
TTCATGGCGG AAAAGCTACT TCAGGAGTAT TGTGCAGAAG TGGGAGGCAA AGATGGGACT 
TTCTTAGTAC GACAAAGTGA ATCTTATCGT GATGATTACA CACTCTCTTT CTGGAGGTCA 
GGCAGGGTCC AACACTGCCG TATTCGTTCC TGTACAGATG GAGAAATCAT AAAGTATTTC 
CTGACCGACA ACCTAATGTT TGATAGTATC TATGATTTGA TTCAACACTA CAAAGAGTAC 
TCATTGCGCT GTGCAGAATT TGAGCTGCGA CTAACAGATG CTGTTCCTCA ACCCAACCCT 
CACTTATCCA AAGACTGGTA CTATGATAAC CTCAGCAGAG GAGAGGCAGA AGATATGTTA 
ATGAGAATCC CTCGGGATGG GGCCTTTTTA ATAAGAAGAA GAGAGGAACA AGATTCCTAT 
GCAATTACTT TTAGAGCTGA AGGGAAAGTG AAGCACTGTA GAATACAAAG GGATGGACGC 
ATGTTTGTAC TAGGAACTTC TGCATACTTT GAAAACCTTG TGGAACTTGT CAGCTATTAC 
CAGAAACACC CGCTATACCG AAAAATGAAA CTACGCTATC CAGTTACAGA AGAGTTACTT 
CAGAGATATA GCACAGAAAA GGATTTAAGT GCCATATATG ATGTAAAGAA TAAACTGTAT 
GTCAATCCTG AAGAAATTTC TCCAGCAATG CCCCAAGGAA CAGTGAAAGC TTTGTACAAC 
TATACAGCTG TAAGATCAGA TGAGCTGAGC TTCTGCACAG GTGCCCTGAT TCATAATGTC 
TCAAGGGTCT CTGAAGGATG GTGGAAAGGA GATTATGGAG ACAAGATACA GTATTTTTTC 
CCCTCAAATT ATGTGGAGGA CATATCAATG AATGCTTCTG AAATAATTGC AAATCAGCTT 
ATTGAAGACA GTCCATTGGG GTCCATGTGC AGAGGTATCC TGGAAATGGG ATCATATAAT 
ATCAGGAAGT TGCTTAATGG AAAATATAAC AAACAGTTTG TTTTGTCCCT GGAATCCAAG 
AATCCAGAAG AGCCTACTGT AGATTTTGCA GCAGATACAA TAGAAGAGTT TTTTGTGTGG 
TACAAACACA TCCAACAGAT TGAACAGAAA AAGGGAGCAG AGGACTTTGA AAAGGGAACA 
CTGGAGAAGC AGCAAGAGAT TGCCCGAGAA TTATCTGATC TGGTTATGTA CTGTAAGCCC 
AAAAGTGAAG CTAAGGAGAA ATTAGAGTCG CCGGATTTTC GGGAAATTCG ATCATTTGCA 
GAGAATAAGG GAGAGAGTAT TGCTCGACAA AAATCCATTG AGTTGTTGCG GTATAACCGT 
AAAGCCTTTA CGCGAATCTA CCCTAAGTCC TCAAGAGTTG ACTCTTCAAA CTATGACCCC 
CTACCCCTAT GGCTCTGTGG AGACCAGATG ATTGCACTAA ATTACCAGAC TCCAGATAGG 
TACATGCAAA TAAATAATGC AATGTTCGCT TACAATGGAG AATGCGGCTA CATCCTGCAG 
CCAGAGTGTA TGAGGGAAGA GTCATATGAC CCCAGTGACT CTAGAAGACA GCTCCAAATG 
ATTGTGACAA TTAAGGTCTT AGGAGCTCGA CATTTGCCAA AACCAGGGAG AGGTATTGCA 
TGCCCTTTTG TAGAAGTGGA GGTATGCGGT GGGGAATATG ACAACAATAA GTTCAGAACA 
ATTGTTATGA ATGATAATGG CCTCAACCCT GTATGGTCAG GAAGTTCTCA ACAGGCTGAG 
TTTGAGATCC ATGAGCCTGA GATTGCCTTT CTGAGGTTTG TAGTATATGA AGAAGATATG 
TTCAGTGACC CCAACTTCTT AGCTCAGTGC ACCATCCCAG TTAAATGTTT AAAAACAGGT 
TATAGATCAG TTCCACTAAA AAACGGTTAC AGTGAAAACC TAGAGCTTGC TTCTCTGTTG 
GTTTATTGCC AAATACAAAA AGATCTGGAA AAAGTAGAAG AACTGTACTC TTCATCAAAG 
CAACTCCGCA AGCGCCAGAC AGACCTGACC AATGAACTGT TTCTTTACGA CACTCACCAA 
AACATGAGAC ACCCCAGTCA TGAGGCATTT GTGAACGACA GTAAAGGGAA TGCCAGTCCA 
GAGGTGTCCA ACAGGAGGTT AAAGGAAAGA CAGATCAGTA ACAGCAAATT CTACTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcg2 ( XM_018093522.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949011.1 Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X2, mRNA.

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CloneID	OXa36654
Clone ID Related Accession (Same CDS sequence)	XM_018093522.1 , XM_018093520.1 , XM_018093520.2 , XM_018093522.2
Accession Version	XM_018093522.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGCA GGGGGAGTTC TGATTATAGA AGTCTCCCTG AATATGAGAA GAGCCAGATT 
AAGAGAACCC TTGAGCTGGG CACAGTCATG ACTGTCTTCA ACTACAAGAA GTCACAGCCT 
GATCGAAGGA CCATCCAAGT GATAATGGAA ACTGGCCAGG TGGCGTGGTG TAAGACAAAT 
GATAAGATTG AAGGATTCTT GGACCTAATG GAAATAAAGG AAATTCGTCC AGGGAAAAAT 
TCAAAGGATT TTCAACGTGG AAAATGTGAC CGTTGTAAAG AAGACGTTTG CTTCACTATT 
TTTTATGGGA ACCAATTTGT TCTGAATACT TTGAGTTTAG CAGCCGATTC AGTTGACGAT 
GCTGAAAAAT GGAAATGTGG TTTAACTATC TTACTTGAAG AAGTTGCTTC TGCCTCAACA 
CCAGCAATAA TTGAAAGTTG GCTGAGAAAA CAGATATACT CTGTGGATCA GACTAGAAAG 
AACAGTATTA GCTTCCGGGA ACTGAAGACC ATTCTTTCAC AGGCCAACTT CAAAGCTAAT 
AGCATGAGAG TGCTGAAGGA CAAGTTAACG GAGATTGGCA TTCAAAGGGA TGATATTAGT 
TTTGAACATT TTCACGTATT CTACAAAAAG CTCATGTTTG AACAGCAGAA GATGATACTG 
GATGAATTTC GGAAGGATTC TTCAGTGTTT ATTTTGGGAA ATGTTGACCG CCCAGATGGG 
TCAGCTGTCC ACCTGCATGA TTTTCAAAGG TTTCTAGTGC ATGAGCAACA GGAATCCCCA 
GCTCAAGATC TCAGTAAGGT TCGTGAACGG ATGACAAAAT TTGTAGATGA TACTATGAGG 
GAAACAGCTG AACCATTTCT TTTTGTGGAC GAGTTCTTGA CCTACTTATT TTCGAAAGAA 
AACAGCATTT GGGATGAGAA ATATGACACG ATCAGTGTTC AAAATATGAA TAATCCTCTT 
TCACATTACT GGATTGCTTC CTCACATAAT ACGTATTTAA CAGGGGACCA GCTGCGGAGT 
GAATCATCTA CTGAAGCATA TGTGCGCTGT CTGCGCATGG GATGCAGGTG CATTGAATTG 
GACTGCTGGG ATGGACCTGA TGGAAAGCCA ATTATTTATC ATGGATGGAC ACGAACAACA 
AAAATTAAAT TTGATGATGT GGTACAGGCT ATTAGGGACC ATGCATTTGC TACATCTGAG 
TATCCAGTTA TCCTTTCTAT AGAGGAACAT TGCTGTGTTG AACAACAGAG ACACATGGCC 
AAAGTTTTTA AAGAAGTATT AGGAGAGCTT CTTTTGACTA AACCAATAGA AGCTACTGCC 
GATCAGTTGC CTTCTCCCAC ACAACTTAAA GGGAGGATCA TTATTAAGCA TAAAAAACTG 
GAAACAAAGA ATCCTGGGAC AAACACTGAG AATCATAAAG AAGAACAAAA TCTTCAGGGT 
GAGCTGTACA TGTGGGATGG TATTGACCAG AAATGGTCTT CTCGTTACTG TGCTATTGCT 
GGTGATAAAT TATCTTATGG AGATGAAATT GAACAGACTG AAGACAGCGC AGCACCAAAG 
AGAACAATAG ATTTACATCT ACAAGAGAAA TGGTTCCACG GGCGAATGTA TAATGGGCGT 
TTCATGGCGG AAAAGCTACT TCAGGAGTAT TGTGCAGAAG TGGGAGGCAA AGATGGGACT 
TTCTTAGTAC GACAAAGTGA ATCTTATCGT GATGATTACA CACTCTCTTT CTGGAGGTCA 
GGCAGGGTCC AACACTGCCG TATTCGTTCC TGTACAGATG GAGAAATCAT AAAGTATTTC 
CTGACCGACA ACCTAATGTT TGATAGTATC TATGATTTGA TTCAACACTA CAAAGAGTAC 
TCATTGCGCT GTGCAGAATT TGAGCTGCGA CTAACAGATG CTGTTCCTCA ACCCAACCCT 
CACTTATCCA AAGACTGGTA CTATGATAAC CTCAGCAGAG GAGAGGCAGA AGATATGTTA 
ATGAGAATCC CTCGGGATGG GGCCTTTTTA ATAAGAAGAA GAGAGGAACA AGATTCCTAT 
GCAATTACTT TTAGAGCTGA AGGGAAAGTG AAGCACTGTA GAATACAAAG GGATGGACGC 
ATGTTTGTAC TAGGAACTTC TGCATACTTT GAAAACCTTG TGGAACTTGT CAGCTATTAC 
CAGAAACACC CGCTATACCG AAAAATGAAA CTACGCTATC CAGTTACAGA AGAGTTACTT 
CAGAGATATA GCACAGAAAA GGATTTAAGT GCCATATATG ATGTAAAGAA TAAACTGTAT 
GTCAATCCTG AAGAAATTTC TCCAGCAATG CCCCAAGGAA CAGTGAAAGC TTTGTACAAC 
TATACAGCTG TAAGATCAGA TGAGCTGAGC TTCTGCACAG GTGCCCTGAT TCATAATGTC 
TCAAGGGTCT CTGAAGGATG GTGGAAAGGA GATTATGGAG ACAAGATACA GTATTTTTTC 
CCCTCAAATT ATGTGGAGGA CATATCAATG AATGCTTCTG AAATAATTGC AAATCAGCTT 
ATTGAAGACA GTCCATTGGG GTCCATGTGC AGAGGTATCC TGGAAATGGG ATCATATAAT 
ATCAGGAAGT TGCTTAATGG AAAATATAAC AAACAGTTTG TTTTGTCCCT GGAATCCAAG 
AATCCAGAAG AGCCTACTGT AGATTTTGCA GCAGATACAA TAGAAGAGTT TTTTGTGTGG 
TACAAACACA TCCAACAGAT TGAACAGAAA AAGGGAGCAG AGGACTTTGA AAAGGGAACA 
CTGGAGAAGC AGCAAGAGAT TGCCCGAGAA TTATCTGATC TGGTTATGTA CTGTAAGCCC 
AAAAGTGAAG CTAAGGAGAA ATTAGAGTCG CCGGATTTTC GGGAAATTCG ATCATTTGCA 
GAGAATAAGG GAGAGAGTAT TGCTCGACAA AAATCCATTG AGTTGTTGCG GTATAACCGT 
AAAGCCTTTA CGCGAATCTA CCCTAAGTCC TCAAGAGTTG ACTCTTCAAA CTATGACCCC 
CTACCCCTAT GGCTCTGTGG AGACCAGATG ATTGCACTAA ATTACCAGAC TCCAGATAGG 
TACATGCAAA TAAATAATGC AATGTTCGCT TACAATGGAG AATGCGGCTA CATCCTGCAG 
CCAGAGTGTA TGAGGGAAGA GTCATATGAC CCCAGTGACT CTAGAAGACA GCTCCAAATG 
ATTGTGACAA TTAAGGTCTT AGGAGCTCGA CATTTGCCAA AACCAGGGAG AGGTATTGCA 
TGCCCTTTTG TAGAAGTGGA GGTATGCGGT GGGGAATATG ACAACAATAA GTTCAGAACA 
ATTGTTATGA ATGATAATGG CCTCAACCCT GTATGGTCAG GAAGTTCTCA ACAGGCTGAG 
TTTGAGATCC ATGAGCCTGA GATTGCCTTT CTGAGGTTTG TAGTATATGA AGAAGATATG 
TTCAGTGACC CCAACTTCTT AGCTCAGTGC ACCATCCCAG TTAAATGTTT AAAAACAGGT 
TATAGATCAG TTCCACTAAA AAACGGTTAC AGTGAAAACC TAGAGCTTGC TTCTCTGTTG 
GTTTATTGCC AAATACAAAA AGATCTGGAA AAAGTAGAAG AACTGTACTC TTCATCAAAG 
CAACTCCGCA AGCGCCAGAC AGACCTGACC AATGAACTGT TTCTTTACGA CACTCACCAA 
AACATGAGAC ACCCCAGTCA TGAGGCATTT GTGAACGACA GTAAAGGGAA TGCCAGTCCA 
GAGGTGTCCA ACAGGAGGTA TAAAATGAGA AAAGATCAGA TTGAATATAT GATCCATATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949011.1
CDS	160..3942
Translation	MDRRGSSDYRSLPEYEKSQIKRTLELGTVMTVFNYKKSQPDRRTIQVIMETGQVAWCKTNDKIEGFLDLMEIKEIRPGKNSKDFQRGKCDRCKEDVCFTIFYGNQFVLNTLSLAADSVDDAEKWKCGLTILLEEVASASTPAIIESWLRKQIYSVDQTRKNSISFRELKTILSQANFKANSMRVLKDKLTEIGIQRDDISFEHFHVFYKKLMFEQQKMILDEFRKDSSVFILGNVDRPDGSAVHLHDFQRFLVHEQQESPAQDLSKVRERMTKFVDDTMRETAEPFLFVDEFLTYLFSKENSIWDEKYDTISVQNMNNPLSHYWIASSHNTYLTGDQLRSESSTEAYVRCLRMGCRCIELDCWDGPDGKPIIYHGWTRTTKIKFDDVVQAIRDHAFATSEYPVILSIEEHCCVEQQRHMAKVFKEVLGELLLTKPIEATADQLPSPTQLKGRIIIKHKKLETKNPGTNTENHKEEQNLQGELYMWDGIDQKWSSRYCAIAGDKLSYGDEIEQTEDSAAPKRTIDLHLQEKWFHGRMYNGRFMAEKLLQEYCAEVGGKDGTFLVRQSESYRDDYTLSFWRSGRVQHCRIRSCTDGEIIKYFLTDNLMFDSIYDLIQHYKEYSLRCAEFELRLTDAVPQPNPHLSKDWYYDNLSRGEAEDMLMRIPRDGAFLIRRREEQDSYAITFRAEGKVKHCRIQRDGRMFVLGTSAYFENLVELVSYYQKHPLYRKMKLRYPVTEELLQRYSTEKDLSAIYDVKNKLYVNPEEISPAMPQGTVKALYNYTAVRSDELSFCTGALIHNVSRVSEGWWKGDYGDKIQYFFPSNYVEDISMNASEIIANQLIEDSPLGSMCRGILEMGSYNIRKLLNGKYNKQFVLSLESKNPEEPTVDFAADTIEEFFVWYKHIQQIEQKKGAEDFEKGTLEKQQEIARELSDLVMYCKPKSEAKEKLESPDFREIRSFAENKGESIARQKSIELLRYNRKAFTRIYPKSSRVDSSNYDPLPLWLCGDQMIALNYQTPDRYMQINNAMFAYNGECGYILQPECMREESYDPSDSRRQLQMIVTIKVLGARHLPKPGRGIACPFVEVEVCGGEYDNNKFRTIVMNDNGLNPVWSGSSQQAEFEIHEPEIAFLRFVVYEEDMFSDPNFLAQCTIPVKCLKTGYRSVPLKNGYSENLELASLLVYCQIQKDLEKVEELYSSSKQLRKRQTDLTNELFLYDTHQNMRHPSHEAFVNDSKGNASPEVSNRRYKMRKDQIEYMIHI

Target ORF information:

RefSeq Version	XM_018093522.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093522.1

ORF Insert Sequence:

ATGGATCGCA GGGGGAGTTC TGATTATAGA AGTCTCCCTG AATATGAGAA GAGCCAGATT 
AAGAGAACCC TTGAGCTGGG CACAGTCATG ACTGTCTTCA ACTACAAGAA GTCACAGCCT 
GATCGAAGGA CCATCCAAGT GATAATGGAA ACTGGCCAGG TGGCGTGGTG TAAGACAAAT 
GATAAGATTG AAGGATTCTT GGACCTAATG GAAATAAAGG AAATTCGTCC AGGGAAAAAT 
TCAAAGGATT TTCAACGTGG AAAATGTGAC CGTTGTAAAG AAGACGTTTG CTTCACTATT 
TTTTATGGGA ACCAATTTGT TCTGAATACT TTGAGTTTAG CAGCCGATTC AGTTGACGAT 
GCTGAAAAAT GGAAATGTGG TTTAACTATC TTACTTGAAG AAGTTGCTTC TGCCTCAACA 
CCAGCAATAA TTGAAAGTTG GCTGAGAAAA CAGATATACT CTGTGGATCA GACTAGAAAG 
AACAGTATTA GCTTCCGGGA ACTGAAGACC ATTCTTTCAC AGGCCAACTT CAAAGCTAAT 
AGCATGAGAG TGCTGAAGGA CAAGTTAACG GAGATTGGCA TTCAAAGGGA TGATATTAGT 
TTTGAACATT TTCACGTATT CTACAAAAAG CTCATGTTTG AACAGCAGAA GATGATACTG 
GATGAATTTC GGAAGGATTC TTCAGTGTTT ATTTTGGGAA ATGTTGACCG CCCAGATGGG 
TCAGCTGTCC ACCTGCATGA TTTTCAAAGG TTTCTAGTGC ATGAGCAACA GGAATCCCCA 
GCTCAAGATC TCAGTAAGGT TCGTGAACGG ATGACAAAAT TTGTAGATGA TACTATGAGG 
GAAACAGCTG AACCATTTCT TTTTGTGGAC GAGTTCTTGA CCTACTTATT TTCGAAAGAA 
AACAGCATTT GGGATGAGAA ATATGACACG ATCAGTGTTC AAAATATGAA TAATCCTCTT 
TCACATTACT GGATTGCTTC CTCACATAAT ACGTATTTAA CAGGGGACCA GCTGCGGAGT 
GAATCATCTA CTGAAGCATA TGTGCGCTGT CTGCGCATGG GATGCAGGTG CATTGAATTG 
GACTGCTGGG ATGGACCTGA TGGAAAGCCA ATTATTTATC ATGGATGGAC ACGAACAACA 
AAAATTAAAT TTGATGATGT GGTACAGGCT ATTAGGGACC ATGCATTTGC TACATCTGAG 
TATCCAGTTA TCCTTTCTAT AGAGGAACAT TGCTGTGTTG AACAACAGAG ACACATGGCC 
AAAGTTTTTA AAGAAGTATT AGGAGAGCTT CTTTTGACTA AACCAATAGA AGCTACTGCC 
GATCAGTTGC CTTCTCCCAC ACAACTTAAA GGGAGGATCA TTATTAAGCA TAAAAAACTG 
GAAACAAAGA ATCCTGGGAC AAACACTGAG AATCATAAAG AAGAACAAAA TCTTCAGGGT 
GAGCTGTACA TGTGGGATGG TATTGACCAG AAATGGTCTT CTCGTTACTG TGCTATTGCT 
GGTGATAAAT TATCTTATGG AGATGAAATT GAACAGACTG AAGACAGCGC AGCACCAAAG 
AGAACAATAG ATTTACATCT ACAAGAGAAA TGGTTCCACG GGCGAATGTA TAATGGGCGT 
TTCATGGCGG AAAAGCTACT TCAGGAGTAT TGTGCAGAAG TGGGAGGCAA AGATGGGACT 
TTCTTAGTAC GACAAAGTGA ATCTTATCGT GATGATTACA CACTCTCTTT CTGGAGGTCA 
GGCAGGGTCC AACACTGCCG TATTCGTTCC TGTACAGATG GAGAAATCAT AAAGTATTTC 
CTGACCGACA ACCTAATGTT TGATAGTATC TATGATTTGA TTCAACACTA CAAAGAGTAC 
TCATTGCGCT GTGCAGAATT TGAGCTGCGA CTAACAGATG CTGTTCCTCA ACCCAACCCT 
CACTTATCCA AAGACTGGTA CTATGATAAC CTCAGCAGAG GAGAGGCAGA AGATATGTTA 
ATGAGAATCC CTCGGGATGG GGCCTTTTTA ATAAGAAGAA GAGAGGAACA AGATTCCTAT 
GCAATTACTT TTAGAGCTGA AGGGAAAGTG AAGCACTGTA GAATACAAAG GGATGGACGC 
ATGTTTGTAC TAGGAACTTC TGCATACTTT GAAAACCTTG TGGAACTTGT CAGCTATTAC 
CAGAAACACC CGCTATACCG AAAAATGAAA CTACGCTATC CAGTTACAGA AGAGTTACTT 
CAGAGATATA GCACAGAAAA GGATTTAAGT GCCATATATG ATGTAAAGAA TAAACTGTAT 
GTCAATCCTG AAGAAATTTC TCCAGCAATG CCCCAAGGAA CAGTGAAAGC TTTGTACAAC 
TATACAGCTG TAAGATCAGA TGAGCTGAGC TTCTGCACAG GTGCCCTGAT TCATAATGTC 
TCAAGGGTCT CTGAAGGATG GTGGAAAGGA GATTATGGAG ACAAGATACA GTATTTTTTC 
CCCTCAAATT ATGTGGAGGA CATATCAATG AATGCTTCTG AAATAATTGC AAATCAGCTT 
ATTGAAGACA GTCCATTGGG GTCCATGTGC AGAGGTATCC TGGAAATGGG ATCATATAAT 
ATCAGGAAGT TGCTTAATGG AAAATATAAC AAACAGTTTG TTTTGTCCCT GGAATCCAAG 
AATCCAGAAG AGCCTACTGT AGATTTTGCA GCAGATACAA TAGAAGAGTT TTTTGTGTGG 
TACAAACACA TCCAACAGAT TGAACAGAAA AAGGGAGCAG AGGACTTTGA AAAGGGAACA 
CTGGAGAAGC AGCAAGAGAT TGCCCGAGAA TTATCTGATC TGGTTATGTA CTGTAAGCCC 
AAAAGTGAAG CTAAGGAGAA ATTAGAGTCG CCGGATTTTC GGGAAATTCG ATCATTTGCA 
GAGAATAAGG GAGAGAGTAT TGCTCGACAA AAATCCATTG AGTTGTTGCG GTATAACCGT 
AAAGCCTTTA CGCGAATCTA CCCTAAGTCC TCAAGAGTTG ACTCTTCAAA CTATGACCCC 
CTACCCCTAT GGCTCTGTGG AGACCAGATG ATTGCACTAA ATTACCAGAC TCCAGATAGG 
TACATGCAAA TAAATAATGC AATGTTCGCT TACAATGGAG AATGCGGCTA CATCCTGCAG 
CCAGAGTGTA TGAGGGAAGA GTCATATGAC CCCAGTGACT CTAGAAGACA GCTCCAAATG 
ATTGTGACAA TTAAGGTCTT AGGAGCTCGA CATTTGCCAA AACCAGGGAG AGGTATTGCA 
TGCCCTTTTG TAGAAGTGGA GGTATGCGGT GGGGAATATG ACAACAATAA GTTCAGAACA 
ATTGTTATGA ATGATAATGG CCTCAACCCT GTATGGTCAG GAAGTTCTCA ACAGGCTGAG 
TTTGAGATCC ATGAGCCTGA GATTGCCTTT CTGAGGTTTG TAGTATATGA AGAAGATATG 
TTCAGTGACC CCAACTTCTT AGCTCAGTGC ACCATCCCAG TTAAATGTTT AAAAACAGGT 
TATAGATCAG TTCCACTAAA AAACGGTTAC AGTGAAAACC TAGAGCTTGC TTCTCTGTTG 
GTTTATTGCC AAATACAAAA AGATCTGGAA AAAGTAGAAG AACTGTACTC TTCATCAAAG 
CAACTCCGCA AGCGCCAGAC AGACCTGACC AATGAACTGT TTCTTTACGA CACTCACCAA 
AACATGAGAC ACCCCAGTCA TGAGGCATTT GTGAACGACA GTAAAGGGAA TGCCAGTCCA 
GAGGTGTCCA ACAGGAGGTA TAAAATGAGA AAAGATCAGA TTGAATATAT GATCCATATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcg2 ( XM_018093520.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949009.1 Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa36654
Clone ID Related Accession (Same CDS sequence)	XM_018093522.1 , XM_018093520.1 , XM_018093520.2 , XM_018093522.2
Accession Version	XM_018093520.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGCA GGGGGAGTTC TGATTATAGA AGTCTCCCTG AATATGAGAA GAGCCAGATT 
AAGAGAACCC TTGAGCTGGG CACAGTCATG ACTGTCTTCA ACTACAAGAA GTCACAGCCT 
GATCGAAGGA CCATCCAAGT GATAATGGAA ACTGGCCAGG TGGCGTGGTG TAAGACAAAT 
GATAAGATTG AAGGATTCTT GGACCTAATG GAAATAAAGG AAATTCGTCC AGGGAAAAAT 
TCAAAGGATT TTCAACGTGG AAAATGTGAC CGTTGTAAAG AAGACGTTTG CTTCACTATT 
TTTTATGGGA ACCAATTTGT TCTGAATACT TTGAGTTTAG CAGCCGATTC AGTTGACGAT 
GCTGAAAAAT GGAAATGTGG TTTAACTATC TTACTTGAAG AAGTTGCTTC TGCCTCAACA 
CCAGCAATAA TTGAAAGTTG GCTGAGAAAA CAGATATACT CTGTGGATCA GACTAGAAAG 
AACAGTATTA GCTTCCGGGA ACTGAAGACC ATTCTTTCAC AGGCCAACTT CAAAGCTAAT 
AGCATGAGAG TGCTGAAGGA CAAGTTAACG GAGATTGGCA TTCAAAGGGA TGATATTAGT 
TTTGAACATT TTCACGTATT CTACAAAAAG CTCATGTTTG AACAGCAGAA GATGATACTG 
GATGAATTTC GGAAGGATTC TTCAGTGTTT ATTTTGGGAA ATGTTGACCG CCCAGATGGG 
TCAGCTGTCC ACCTGCATGA TTTTCAAAGG TTTCTAGTGC ATGAGCAACA GGAATCCCCA 
GCTCAAGATC TCAGTAAGGT TCGTGAACGG ATGACAAAAT TTGTAGATGA TACTATGAGG 
GAAACAGCTG AACCATTTCT TTTTGTGGAC GAGTTCTTGA CCTACTTATT TTCGAAAGAA 
AACAGCATTT GGGATGAGAA ATATGACACG ATCAGTGTTC AAAATATGAA TAATCCTCTT 
TCACATTACT GGATTGCTTC CTCACATAAT ACGTATTTAA CAGGGGACCA GCTGCGGAGT 
GAATCATCTA CTGAAGCATA TGTGCGCTGT CTGCGCATGG GATGCAGGTG CATTGAATTG 
GACTGCTGGG ATGGACCTGA TGGAAAGCCA ATTATTTATC ATGGATGGAC ACGAACAACA 
AAAATTAAAT TTGATGATGT GGTACAGGCT ATTAGGGACC ATGCATTTGC TACATCTGAG 
TATCCAGTTA TCCTTTCTAT AGAGGAACAT TGCTGTGTTG AACAACAGAG ACACATGGCC 
AAAGTTTTTA AAGAAGTATT AGGAGAGCTT CTTTTGACTA AACCAATAGA AGCTACTGCC 
GATCAGTTGC CTTCTCCCAC ACAACTTAAA GGGAGGATCA TTATTAAGCA TAAAAAACTG 
GAAACAAAGA ATCCTGGGAC AAACACTGAG AATCATAAAG AAGAACAAAA TCTTCAGGGT 
GAGCTGTACA TGTGGGATGG TATTGACCAG AAATGGTCTT CTCGTTACTG TGCTATTGCT 
GGTGATAAAT TATCTTATGG AGATGAAATT GAACAGACTG AAGACAGCGC AGCACCAAAG 
AGAACAATAG ATTTACATCT ACAAGAGAAA TGGTTCCACG GGCGAATGTA TAATGGGCGT 
TTCATGGCGG AAAAGCTACT TCAGGAGTAT TGTGCAGAAG TGGGAGGCAA AGATGGGACT 
TTCTTAGTAC GACAAAGTGA ATCTTATCGT GATGATTACA CACTCTCTTT CTGGAGGTCA 
GGCAGGGTCC AACACTGCCG TATTCGTTCC TGTACAGATG GAGAAATCAT AAAGTATTTC 
CTGACCGACA ACCTAATGTT TGATAGTATC TATGATTTGA TTCAACACTA CAAAGAGTAC 
TCATTGCGCT GTGCAGAATT TGAGCTGCGA CTAACAGATG CTGTTCCTCA ACCCAACCCT 
CACTTATCCA AAGACTGGTA CTATGATAAC CTCAGCAGAG GAGAGGCAGA AGATATGTTA 
ATGAGAATCC CTCGGGATGG GGCCTTTTTA ATAAGAAGAA GAGAGGAACA AGATTCCTAT 
GCAATTACTT TTAGAGCTGA AGGGAAAGTG AAGCACTGTA GAATACAAAG GGATGGACGC 
ATGTTTGTAC TAGGAACTTC TGCATACTTT GAAAACCTTG TGGAACTTGT CAGCTATTAC 
CAGAAACACC CGCTATACCG AAAAATGAAA CTACGCTATC CAGTTACAGA AGAGTTACTT 
CAGAGATATA GCACAGAAAA GGATTTAAGT GCCATATATG ATGTAAAGAA TAAACTGTAT 
GTCAATCCTG AAGAAATTTC TCCAGCAATG CCCCAAGGAA CAGTGAAAGC TTTGTACAAC 
TATACAGCTG TAAGATCAGA TGAGCTGAGC TTCTGCACAG GTGCCCTGAT TCATAATGTC 
TCAAGGGTCT CTGAAGGATG GTGGAAAGGA GATTATGGAG ACAAGATACA GTATTTTTTC 
CCCTCAAATT ATGTGGAGGA CATATCAATG AATGCTTCTG AAATAATTGC AAATCAGCTT 
ATTGAAGACA GTCCATTGGG GTCCATGTGC AGAGGTATCC TGGAAATGGG ATCATATAAT 
ATCAGGAAGT TGCTTAATGG AAAATATAAC AAACAGTTTG TTTTGTCCCT GGAATCCAAG 
AATCCAGAAG AGCCTACTGT AGATTTTGCA GCAGATACAA TAGAAGAGTT TTTTGTGTGG 
TACAAACACA TCCAACAGAT TGAACAGAAA AAGGGAGCAG AGGACTTTGA AAAGGGAACA 
CTGGAGAAGC AGCAAGAGAT TGCCCGAGAA TTATCTGATC TGGTTATGTA CTGTAAGCCC 
AAAAGTGAAG CTAAGGAGAA ATTAGAGTCG CCGGATTTTC GGGAAATTCG ATCATTTGCA 
GAGAATAAGG GAGAGAGTAT TGCTCGACAA AAATCCATTG AGTTGTTGCG GTATAACCGT 
AAAGCCTTTA CGCGAATCTA CCCTAAGTCC TCAAGAGTTG ACTCTTCAAA CTATGACCCC 
CTACCCCTAT GGCTCTGTGG AGACCAGATG ATTGCACTAA ATTACCAGAC TCCAGATAGG 
TACATGCAAA TAAATAATGC AATGTTCGCT TACAATGGAG AATGCGGCTA CATCCTGCAG 
CCAGAGTGTA TGAGGGAAGA GTCATATGAC CCCAGTGACT CTAGAAGACA GCTCCAAATG 
ATTGTGACAA TTAAGGTCTT AGGAGCTCGA CATTTGCCAA AACCAGGGAG AGGTATTGCA 
TGCCCTTTTG TAGAAGTGGA GGTATGCGGT GGGGAATATG ACAACAATAA GTTCAGAACA 
ATTGTTATGA ATGATAATGG CCTCAACCCT GTATGGTCAG GAAGTTCTCA ACAGGCTGAG 
TTTGAGATCC ATGAGCCTGA GATTGCCTTT CTGAGGTTTG TAGTATATGA AGAAGATATG 
TTCAGTGACC CCAACTTCTT AGCTCAGTGC ACCATCCCAG TTAAATGTTT AAAAACAGGT 
TATAGATCAG TTCCACTAAA AAACGGTTAC AGTGAAAACC TAGAGCTTGC TTCTCTGTTG 
GTTTATTGCC AAATACAAAA AGATCTGGAA AAAGTAGAAG AACTGTACTC TTCATCAAAG 
CAACTCCGCA AGCGCCAGAC AGACCTGACC AATGAACTGT TTCTTTACGA CACTCACCAA 
AACATGAGAC ACCCCAGTCA TGAGGCATTT GTGAACGACA GTAAAGGGAA TGCCAGTCCA 
GAGGTGTCCA ACAGGAGGTA TAAAATGAGA AAAGATCAGA TTGAATATAT GATCCATATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949009.1
CDS	268..4050
Translation	MDRRGSSDYRSLPEYEKSQIKRTLELGTVMTVFNYKKSQPDRRTIQVIMETGQVAWCKTNDKIEGFLDLMEIKEIRPGKNSKDFQRGKCDRCKEDVCFTIFYGNQFVLNTLSLAADSVDDAEKWKCGLTILLEEVASASTPAIIESWLRKQIYSVDQTRKNSISFRELKTILSQANFKANSMRVLKDKLTEIGIQRDDISFEHFHVFYKKLMFEQQKMILDEFRKDSSVFILGNVDRPDGSAVHLHDFQRFLVHEQQESPAQDLSKVRERMTKFVDDTMRETAEPFLFVDEFLTYLFSKENSIWDEKYDTISVQNMNNPLSHYWIASSHNTYLTGDQLRSESSTEAYVRCLRMGCRCIELDCWDGPDGKPIIYHGWTRTTKIKFDDVVQAIRDHAFATSEYPVILSIEEHCCVEQQRHMAKVFKEVLGELLLTKPIEATADQLPSPTQLKGRIIIKHKKLETKNPGTNTENHKEEQNLQGELYMWDGIDQKWSSRYCAIAGDKLSYGDEIEQTEDSAAPKRTIDLHLQEKWFHGRMYNGRFMAEKLLQEYCAEVGGKDGTFLVRQSESYRDDYTLSFWRSGRVQHCRIRSCTDGEIIKYFLTDNLMFDSIYDLIQHYKEYSLRCAEFELRLTDAVPQPNPHLSKDWYYDNLSRGEAEDMLMRIPRDGAFLIRRREEQDSYAITFRAEGKVKHCRIQRDGRMFVLGTSAYFENLVELVSYYQKHPLYRKMKLRYPVTEELLQRYSTEKDLSAIYDVKNKLYVNPEEISPAMPQGTVKALYNYTAVRSDELSFCTGALIHNVSRVSEGWWKGDYGDKIQYFFPSNYVEDISMNASEIIANQLIEDSPLGSMCRGILEMGSYNIRKLLNGKYNKQFVLSLESKNPEEPTVDFAADTIEEFFVWYKHIQQIEQKKGAEDFEKGTLEKQQEIARELSDLVMYCKPKSEAKEKLESPDFREIRSFAENKGESIARQKSIELLRYNRKAFTRIYPKSSRVDSSNYDPLPLWLCGDQMIALNYQTPDRYMQINNAMFAYNGECGYILQPECMREESYDPSDSRRQLQMIVTIKVLGARHLPKPGRGIACPFVEVEVCGGEYDNNKFRTIVMNDNGLNPVWSGSSQQAEFEIHEPEIAFLRFVVYEEDMFSDPNFLAQCTIPVKCLKTGYRSVPLKNGYSENLELASLLVYCQIQKDLEKVEELYSSSKQLRKRQTDLTNELFLYDTHQNMRHPSHEAFVNDSKGNASPEVSNRRYKMRKDQIEYMIHI

Target ORF information:

RefSeq Version	XM_018093520.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093520.1

ORF Insert Sequence:

ATGGATCGCA GGGGGAGTTC TGATTATAGA AGTCTCCCTG AATATGAGAA GAGCCAGATT 
AAGAGAACCC TTGAGCTGGG CACAGTCATG ACTGTCTTCA ACTACAAGAA GTCACAGCCT 
GATCGAAGGA CCATCCAAGT GATAATGGAA ACTGGCCAGG TGGCGTGGTG TAAGACAAAT 
GATAAGATTG AAGGATTCTT GGACCTAATG GAAATAAAGG AAATTCGTCC AGGGAAAAAT 
TCAAAGGATT TTCAACGTGG AAAATGTGAC CGTTGTAAAG AAGACGTTTG CTTCACTATT 
TTTTATGGGA ACCAATTTGT TCTGAATACT TTGAGTTTAG CAGCCGATTC AGTTGACGAT 
GCTGAAAAAT GGAAATGTGG TTTAACTATC TTACTTGAAG AAGTTGCTTC TGCCTCAACA 
CCAGCAATAA TTGAAAGTTG GCTGAGAAAA CAGATATACT CTGTGGATCA GACTAGAAAG 
AACAGTATTA GCTTCCGGGA ACTGAAGACC ATTCTTTCAC AGGCCAACTT CAAAGCTAAT 
AGCATGAGAG TGCTGAAGGA CAAGTTAACG GAGATTGGCA TTCAAAGGGA TGATATTAGT 
TTTGAACATT TTCACGTATT CTACAAAAAG CTCATGTTTG AACAGCAGAA GATGATACTG 
GATGAATTTC GGAAGGATTC TTCAGTGTTT ATTTTGGGAA ATGTTGACCG CCCAGATGGG 
TCAGCTGTCC ACCTGCATGA TTTTCAAAGG TTTCTAGTGC ATGAGCAACA GGAATCCCCA 
GCTCAAGATC TCAGTAAGGT TCGTGAACGG ATGACAAAAT TTGTAGATGA TACTATGAGG 
GAAACAGCTG AACCATTTCT TTTTGTGGAC GAGTTCTTGA CCTACTTATT TTCGAAAGAA 
AACAGCATTT GGGATGAGAA ATATGACACG ATCAGTGTTC AAAATATGAA TAATCCTCTT 
TCACATTACT GGATTGCTTC CTCACATAAT ACGTATTTAA CAGGGGACCA GCTGCGGAGT 
GAATCATCTA CTGAAGCATA TGTGCGCTGT CTGCGCATGG GATGCAGGTG CATTGAATTG 
GACTGCTGGG ATGGACCTGA TGGAAAGCCA ATTATTTATC ATGGATGGAC ACGAACAACA 
AAAATTAAAT TTGATGATGT GGTACAGGCT ATTAGGGACC ATGCATTTGC TACATCTGAG 
TATCCAGTTA TCCTTTCTAT AGAGGAACAT TGCTGTGTTG AACAACAGAG ACACATGGCC 
AAAGTTTTTA AAGAAGTATT AGGAGAGCTT CTTTTGACTA AACCAATAGA AGCTACTGCC 
GATCAGTTGC CTTCTCCCAC ACAACTTAAA GGGAGGATCA TTATTAAGCA TAAAAAACTG 
GAAACAAAGA ATCCTGGGAC AAACACTGAG AATCATAAAG AAGAACAAAA TCTTCAGGGT 
GAGCTGTACA TGTGGGATGG TATTGACCAG AAATGGTCTT CTCGTTACTG TGCTATTGCT 
GGTGATAAAT TATCTTATGG AGATGAAATT GAACAGACTG AAGACAGCGC AGCACCAAAG 
AGAACAATAG ATTTACATCT ACAAGAGAAA TGGTTCCACG GGCGAATGTA TAATGGGCGT 
TTCATGGCGG AAAAGCTACT TCAGGAGTAT TGTGCAGAAG TGGGAGGCAA AGATGGGACT 
TTCTTAGTAC GACAAAGTGA ATCTTATCGT GATGATTACA CACTCTCTTT CTGGAGGTCA 
GGCAGGGTCC AACACTGCCG TATTCGTTCC TGTACAGATG GAGAAATCAT AAAGTATTTC 
CTGACCGACA ACCTAATGTT TGATAGTATC TATGATTTGA TTCAACACTA CAAAGAGTAC 
TCATTGCGCT GTGCAGAATT TGAGCTGCGA CTAACAGATG CTGTTCCTCA ACCCAACCCT 
CACTTATCCA AAGACTGGTA CTATGATAAC CTCAGCAGAG GAGAGGCAGA AGATATGTTA 
ATGAGAATCC CTCGGGATGG GGCCTTTTTA ATAAGAAGAA GAGAGGAACA AGATTCCTAT 
GCAATTACTT TTAGAGCTGA AGGGAAAGTG AAGCACTGTA GAATACAAAG GGATGGACGC 
ATGTTTGTAC TAGGAACTTC TGCATACTTT GAAAACCTTG TGGAACTTGT CAGCTATTAC 
CAGAAACACC CGCTATACCG AAAAATGAAA CTACGCTATC CAGTTACAGA AGAGTTACTT 
CAGAGATATA GCACAGAAAA GGATTTAAGT GCCATATATG ATGTAAAGAA TAAACTGTAT 
GTCAATCCTG AAGAAATTTC TCCAGCAATG CCCCAAGGAA CAGTGAAAGC TTTGTACAAC 
TATACAGCTG TAAGATCAGA TGAGCTGAGC TTCTGCACAG GTGCCCTGAT TCATAATGTC 
TCAAGGGTCT CTGAAGGATG GTGGAAAGGA GATTATGGAG ACAAGATACA GTATTTTTTC 
CCCTCAAATT ATGTGGAGGA CATATCAATG AATGCTTCTG AAATAATTGC AAATCAGCTT 
ATTGAAGACA GTCCATTGGG GTCCATGTGC AGAGGTATCC TGGAAATGGG ATCATATAAT 
ATCAGGAAGT TGCTTAATGG AAAATATAAC AAACAGTTTG TTTTGTCCCT GGAATCCAAG 
AATCCAGAAG AGCCTACTGT AGATTTTGCA GCAGATACAA TAGAAGAGTT TTTTGTGTGG 
TACAAACACA TCCAACAGAT TGAACAGAAA AAGGGAGCAG AGGACTTTGA AAAGGGAACA 
CTGGAGAAGC AGCAAGAGAT TGCCCGAGAA TTATCTGATC TGGTTATGTA CTGTAAGCCC 
AAAAGTGAAG CTAAGGAGAA ATTAGAGTCG CCGGATTTTC GGGAAATTCG ATCATTTGCA 
GAGAATAAGG GAGAGAGTAT TGCTCGACAA AAATCCATTG AGTTGTTGCG GTATAACCGT 
AAAGCCTTTA CGCGAATCTA CCCTAAGTCC TCAAGAGTTG ACTCTTCAAA CTATGACCCC 
CTACCCCTAT GGCTCTGTGG AGACCAGATG ATTGCACTAA ATTACCAGAC TCCAGATAGG 
TACATGCAAA TAAATAATGC AATGTTCGCT TACAATGGAG AATGCGGCTA CATCCTGCAG 
CCAGAGTGTA TGAGGGAAGA GTCATATGAC CCCAGTGACT CTAGAAGACA GCTCCAAATG 
ATTGTGACAA TTAAGGTCTT AGGAGCTCGA CATTTGCCAA AACCAGGGAG AGGTATTGCA 
TGCCCTTTTG TAGAAGTGGA GGTATGCGGT GGGGAATATG ACAACAATAA GTTCAGAACA 
ATTGTTATGA ATGATAATGG CCTCAACCCT GTATGGTCAG GAAGTTCTCA ACAGGCTGAG 
TTTGAGATCC ATGAGCCTGA GATTGCCTTT CTGAGGTTTG TAGTATATGA AGAAGATATG 
TTCAGTGACC CCAACTTCTT AGCTCAGTGC ACCATCCCAG TTAAATGTTT AAAAACAGGT 
TATAGATCAG TTCCACTAAA AAACGGTTAC AGTGAAAACC TAGAGCTTGC TTCTCTGTTG 
GTTTATTGCC AAATACAAAA AGATCTGGAA AAAGTAGAAG AACTGTACTC TTCATCAAAG 
CAACTCCGCA AGCGCCAGAC AGACCTGACC AATGAACTGT TTCTTTACGA CACTCACCAA 
AACATGAGAC ACCCCAGTCA TGAGGCATTT GTGAACGACA GTAAAGGGAA TGCCAGTCCA 
GAGGTGTCCA ACAGGAGGTA TAAAATGAGA AAAGATCAGA TTGAATATAT GATCCATATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcg2 ( XM_012962308.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817762.1 Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa36655
Clone ID Related Accession (Same CDS sequence)	XM_012962308.2 , XM_012962308.3
Accession Version	XM_012962308.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962308.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGCA GGGGGAGTTC TGATTATAGA AGTCTCCCTG AATATGAGAA GAGCCAGATT 
AAGAGAACCC TTGAGCTGGG CACAGTCATG ACTGTCTTCA ACTACAAGAA GTCACAGCCT 
GATCGAAGGA CCATCCAAGT GATAATGGAA ACTGGCCAGG TGGCGTGGTG TAAGACAAAT 
GATAAGATTG AAGGATTCTT GGACCTAATG GAAATAAAGG AAATTCGTCC AGGGAAAAAT 
TCAAAGGATT TTCAACGTGG AAAATGTGAC CGTTGTAAAG AAGACGTTTG CTTCACTATT 
TTTTATGGGA ACCAATTTGT TCTGAATACT TTGAGTTTAG CAGCCGATTC AGTTGACGAT 
GCTGAAAAAT GGAAATGTGG TTTAACTATC TTACTTGAAG AAGTTGCTTC TGCCTCAACA 
CCAGCAATAA TTGAAAGTTG GCTGAGAAAA CAGATATACT CTGTGGATCA GACTAGAAAG 
AACAGTATTA GCTTCCGGGA ACTGAAGACC ATTCTTTCAC AGGCCAACTT CAAAGCTAAT 
AGCATGAGAG TGCTGAAGGA CAAGTTAACG GAGATTGGCA TTCAAAGGGA TGATATTAGT 
TTTGAACATT TTCACGTATT CTACAAAAAG CTCATGTTTG AACAGCAGAA GATGATACTG 
GATGAATTTC GGAAGGATTC TTCAGTGTTT ATTTTGGGAA ATGTTGACCG CCCAGATGGG 
TCAGCTGTCC ACCTGCATGA TTTTCAAAGG TTTCTAGTGC ATGAGCAACA GGAATCCCCA 
GCTCAAGATC TCAGTAAGGT TCGTGAACGG ATGACAAAAT TTGTAGATGA TACTATGAGG 
GAAACAGCTG AACCATTTCT TTTTGTGGAC GAGTTCTTGA CCTACTTATT TTCGAAAGAA 
AACAGCATTT GGGATGAGAA ATATGACACG ATCAGTGTTC AAAATATGAA TAATCCTCTT 
TCACATTACT GGATTGCTTC CTCACATAAT ACGTATTTAA CAGGGGACCA GCTGCGGAGT 
GAATCATCTA CTGAAGCATA TGTGCGCTGT CTGCGCATGG GATGCAGGTG CATTGAATTG 
GACTGCTGGG ATGGACCTGA TGGAAAGCCA ATTATTTATC ATGGATGGAC ACGAACAACA 
AAAATTAAAT TTGATGATGT GGTACAGGCT ATTAGGGACC ATGCATTTGC TACATCTGAG 
TATCCAGTTA TCCTTTCTAT AGAGGAACAT TGCTGTGTTG AACAACAGAG ACACATGGCC 
AAAGTTTTTA AAGAAGTATT AGGAGAGCTT CTTTTGACTA AACCAATAGA AGCTACTGCC 
GATCAGTTGC CTTCTCCCAC ACAACTTAAA GGGAGGATCA TTATTAAGCA TAAAAAACTG 
GAAACAAAGA ATCCTGGGAC AAACACTGAG AATCATAAAG AAGAACAAAA TCTTCAGGGT 
GAGCTGTACA TGTGGGATGG TATTGACCAG AAATGGTCTT CTCGTTACTG TGCTATTGCT 
GGTGATAAAT TATCTTATGG AGATGAAATT GAACAGACTG AAGACAGCGC AGCACCAAAG 
AGAACAATAG ATTTACATCT ACAAGAGAAA TGGTTCCACG GGCGAATGTA TAATGGGCGT 
TTCATGGCGG AAAAGCTACT TCAGGAGTAT TGTGCAGAAG TGGGAGGCAA AGATGGGACT 
TTCTTAGTAC GACAAAGTGA ATCTTATCGT GATGATTACA CACTCTCTTT CTGGAGGTCA 
GGCAGGGTCC AACACTGCCG TATTCGTTCC TGTACAGATG GAGAAATCAT AAAGTATTTC 
CTGACCGACA ACCTAATGTT TGATAGTATC TATGATTTGA TTCAACACTA CAAAGAGTAC 
TCATTGCGCT GTGCAGAATT TGAGCTGCGA CTAACAGATG CTGTTCCTCA ACCCAACCCT 
CACTTATCCA AAGACTGGTA CTATGATAAC CTCAGCAGAG GAGAGGCAGA AGATATGTTA 
ATGAGAATCC CTCGGGATGG GGCCTTTTTA ATAAGAAGAA GAGAGGAACA AGATTCCTAT 
GCAATTACTT TTAGAGCTGA AGGGAAAGTG AAGCACTGTA GAATACAAAG GGATGGACGC 
ATGTTTGTAC TAGGAACTTC TGCATACTTT GAAAACCTTG TGGAACTTGT CAGCTATTAC 
CAGAAACACC CGCTATACCG AAAAATGAAA CTACGCTATC CAGTTACAGA AGAGTTACTT 
CAGAGATATA GCACAGAAAA GGATTTAAGT GCCATATATG ATGTAAAGAA TAAACTGTAT 
GTCAATCCTG AAGAAATTTC TCCAGCAATG CCCCAAGGAA CAGTGAAAGC TTTGTACAAC 
TATACAGCTG TAAGATCAGA TGAGCTGAGC TTCTGCACAG GTGCCCTGAT TCATAATGTC 
TCAAGGGTCT CTGAAGGATG GTGGAAAGGA GATTATGGAG ACAAGATACA GTATTTTTTC 
CCCTCAAATT ATGTGGAGGA CATATCAATG AATGCTTCTG AAATAATTGC AAATCAGCTT 
ATTGAAGACA GTCCATTGGG GTCCATGTGC AGAGGTATCC TGGAAATGGG ATCATATAAT 
ATCAGGAAGT TGCTTAATGG AAAATATAAC AAACAGTTTG TTTTGTCCCT GGAATCCAAG 
AATCCAGAAG AGCCTACTGT AGATTTTGCA GCAGATACAA TAGAAGAGTT TTTTGTGTGG 
TACAAACACA TCCAACAGAT TGAACAGAAA AAGGGAGCAG AGGACTTTGA AAAGGGAACA 
CTGGAGAAGC AGCAAGAGAT TGCCCGAGAA TTATCTGATC TGGTTATGTA CTGTAAGCCC 
AAAAGTGAAG CTAAGGAGAA ATTAGAGTCG CCGGATTTTC GGGAAATTCG ATCATTTGCA 
GAGAATAAGG GAGAGAGTAT TGCTCGACAA AAATCCATTG AGTTGTTGCG GTATAACCGT 
AAAGCCTTTA CGCGAATCTA CCCTAAGTCC TCAAGAGTTG ACTCTTCAAA CTATGACCCC 
CTACCCCTAT GGCTCTGTGG AGACCAGATG ATTGCACTAA ATTACCAGAC TCCAGATAGG 
TACATGCAAA TAAATAATGC AATGTTCGCT TACAATGGAG AATGCGGCTA CATCCTGCAG 
CCAGAGTGTA TGAGGGAAGA GTCATATGAC CCCAGTGACT CTAGAAGACA GCTCCAAATG 
ATTGTGACAA TTAAGGTCTT AGGAGCTCGA CATTTGCCAA AACCAGGGAG AGGTATTGCA 
TGCCCTTTTG TAGAAGTGGA GGTATGCGGT GGGGAATATG ACAACAATAA GTTCAGAACA 
ATTGTTATGA ATGATAATGG CCTCAACCCT GTATGGTCAG GAAGTTCTCA ACAGGCTGAG 
TTTGAGATCC ATGAGCCTGA GATTGCCTTT CTGAGGTTTG TAGTATATGA AGAAGATATG 
TTCAGTGACC CCAACTTCTT AGCTCAGTGC ACCATCCCAG TTAAATGTTT AAAAACAGGT 
TATAGATCAG TTCCACTAAA AAACGGTTAC AGTGAAAACC TAGAGCTTGC TTCTCTGTTG 
GTTTATTGCC AAATACAAAA AGATCTGACT CGCCCACGTG CAAAATTCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817762.1
CDS	268..3861
Translation	MDRRGSSDYRSLPEYEKSQIKRTLELGTVMTVFNYKKSQPDRRTIQVIMETGQVAWCKTNDKIEGFLDLMEIKEIRPGKNSKDFQRGKCDRCKEDVCFTIFYGNQFVLNTLSLAADSVDDAEKWKCGLTILLEEVASASTPAIIESWLRKQIYSVDQTRKNSISFRELKTILSQANFKANSMRVLKDKLTEIGIQRDDISFEHFHVFYKKLMFEQQKMILDEFRKDSSVFILGNVDRPDGSAVHLHDFQRFLVHEQQESPAQDLSKVRERMTKFVDDTMRETAEPFLFVDEFLTYLFSKENSIWDEKYDTISVQNMNNPLSHYWIASSHNTYLTGDQLRSESSTEAYVRCLRMGCRCIELDCWDGPDGKPIIYHGWTRTTKIKFDDVVQAIRDHAFATSEYPVILSIEEHCCVEQQRHMAKVFKEVLGELLLTKPIEATADQLPSPTQLKGRIIIKHKKLETKNPGTNTENHKEEQNLQGELYMWDGIDQKWSSRYCAIAGDKLSYGDEIEQTEDSAAPKRTIDLHLQEKWFHGRMYNGRFMAEKLLQEYCAEVGGKDGTFLVRQSESYRDDYTLSFWRSGRVQHCRIRSCTDGEIIKYFLTDNLMFDSIYDLIQHYKEYSLRCAEFELRLTDAVPQPNPHLSKDWYYDNLSRGEAEDMLMRIPRDGAFLIRRREEQDSYAITFRAEGKVKHCRIQRDGRMFVLGTSAYFENLVELVSYYQKHPLYRKMKLRYPVTEELLQRYSTEKDLSAIYDVKNKLYVNPEEISPAMPQGTVKALYNYTAVRSDELSFCTGALIHNVSRVSEGWWKGDYGDKIQYFFPSNYVEDISMNASEIIANQLIEDSPLGSMCRGILEMGSYNIRKLLNGKYNKQFVLSLESKNPEEPTVDFAADTIEEFFVWYKHIQQIEQKKGAEDFEKGTLEKQQEIARELSDLVMYCKPKSEAKEKLESPDFREIRSFAENKGESIARQKSIELLRYNRKAFTRIYPKSSRVDSSNYDPLPLWLCGDQMIALNYQTPDRYMQINNAMFAYNGECGYILQPECMREESYDPSDSRRQLQMIVTIKVLGARHLPKPGRGIACPFVEVEVCGGEYDNNKFRTIVMNDNGLNPVWSGSSQQAEFEIHEPEIAFLRFVVYEEDMFSDPNFLAQCTIPVKCLKTGYRSVPLKNGYSENLELASLLVYCQIQKDLTRPRAKFR

Target ORF information:

RefSeq Version	XM_012962308.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C gamma 2 (plcg2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962308.2

ORF Insert Sequence:

ATGGATCGCA GGGGGAGTTC TGATTATAGA AGTCTCCCTG AATATGAGAA GAGCCAGATT 
AAGAGAACCC TTGAGCTGGG CACAGTCATG ACTGTCTTCA ACTACAAGAA GTCACAGCCT 
GATCGAAGGA CCATCCAAGT GATAATGGAA ACTGGCCAGG TGGCGTGGTG TAAGACAAAT 
GATAAGATTG AAGGATTCTT GGACCTAATG GAAATAAAGG AAATTCGTCC AGGGAAAAAT 
TCAAAGGATT TTCAACGTGG AAAATGTGAC CGTTGTAAAG AAGACGTTTG CTTCACTATT 
TTTTATGGGA ACCAATTTGT TCTGAATACT TTGAGTTTAG CAGCCGATTC AGTTGACGAT 
GCTGAAAAAT GGAAATGTGG TTTAACTATC TTACTTGAAG AAGTTGCTTC TGCCTCAACA 
CCAGCAATAA TTGAAAGTTG GCTGAGAAAA CAGATATACT CTGTGGATCA GACTAGAAAG 
AACAGTATTA GCTTCCGGGA ACTGAAGACC ATTCTTTCAC AGGCCAACTT CAAAGCTAAT 
AGCATGAGAG TGCTGAAGGA CAAGTTAACG GAGATTGGCA TTCAAAGGGA TGATATTAGT 
TTTGAACATT TTCACGTATT CTACAAAAAG CTCATGTTTG AACAGCAGAA GATGATACTG 
GATGAATTTC GGAAGGATTC TTCAGTGTTT ATTTTGGGAA ATGTTGACCG CCCAGATGGG 
TCAGCTGTCC ACCTGCATGA TTTTCAAAGG TTTCTAGTGC ATGAGCAACA GGAATCCCCA 
GCTCAAGATC TCAGTAAGGT TCGTGAACGG ATGACAAAAT TTGTAGATGA TACTATGAGG 
GAAACAGCTG AACCATTTCT TTTTGTGGAC GAGTTCTTGA CCTACTTATT TTCGAAAGAA 
AACAGCATTT GGGATGAGAA ATATGACACG ATCAGTGTTC AAAATATGAA TAATCCTCTT 
TCACATTACT GGATTGCTTC CTCACATAAT ACGTATTTAA CAGGGGACCA GCTGCGGAGT 
GAATCATCTA CTGAAGCATA TGTGCGCTGT CTGCGCATGG GATGCAGGTG CATTGAATTG 
GACTGCTGGG ATGGACCTGA TGGAAAGCCA ATTATTTATC ATGGATGGAC ACGAACAACA 
AAAATTAAAT TTGATGATGT GGTACAGGCT ATTAGGGACC ATGCATTTGC TACATCTGAG 
TATCCAGTTA TCCTTTCTAT AGAGGAACAT TGCTGTGTTG AACAACAGAG ACACATGGCC 
AAAGTTTTTA AAGAAGTATT AGGAGAGCTT CTTTTGACTA AACCAATAGA AGCTACTGCC 
GATCAGTTGC CTTCTCCCAC ACAACTTAAA GGGAGGATCA TTATTAAGCA TAAAAAACTG 
GAAACAAAGA ATCCTGGGAC AAACACTGAG AATCATAAAG AAGAACAAAA TCTTCAGGGT 
GAGCTGTACA TGTGGGATGG TATTGACCAG AAATGGTCTT CTCGTTACTG TGCTATTGCT 
GGTGATAAAT TATCTTATGG AGATGAAATT GAACAGACTG AAGACAGCGC AGCACCAAAG 
AGAACAATAG ATTTACATCT ACAAGAGAAA TGGTTCCACG GGCGAATGTA TAATGGGCGT 
TTCATGGCGG AAAAGCTACT TCAGGAGTAT TGTGCAGAAG TGGGAGGCAA AGATGGGACT 
TTCTTAGTAC GACAAAGTGA ATCTTATCGT GATGATTACA CACTCTCTTT CTGGAGGTCA 
GGCAGGGTCC AACACTGCCG TATTCGTTCC TGTACAGATG GAGAAATCAT AAAGTATTTC 
CTGACCGACA ACCTAATGTT TGATAGTATC TATGATTTGA TTCAACACTA CAAAGAGTAC 
TCATTGCGCT GTGCAGAATT TGAGCTGCGA CTAACAGATG CTGTTCCTCA ACCCAACCCT 
CACTTATCCA AAGACTGGTA CTATGATAAC CTCAGCAGAG GAGAGGCAGA AGATATGTTA 
ATGAGAATCC CTCGGGATGG GGCCTTTTTA ATAAGAAGAA GAGAGGAACA AGATTCCTAT 
GCAATTACTT TTAGAGCTGA AGGGAAAGTG AAGCACTGTA GAATACAAAG GGATGGACGC 
ATGTTTGTAC TAGGAACTTC TGCATACTTT GAAAACCTTG TGGAACTTGT CAGCTATTAC 
CAGAAACACC CGCTATACCG AAAAATGAAA CTACGCTATC CAGTTACAGA AGAGTTACTT 
CAGAGATATA GCACAGAAAA GGATTTAAGT GCCATATATG ATGTAAAGAA TAAACTGTAT 
GTCAATCCTG AAGAAATTTC TCCAGCAATG CCCCAAGGAA CAGTGAAAGC TTTGTACAAC 
TATACAGCTG TAAGATCAGA TGAGCTGAGC TTCTGCACAG GTGCCCTGAT TCATAATGTC 
TCAAGGGTCT CTGAAGGATG GTGGAAAGGA GATTATGGAG ACAAGATACA GTATTTTTTC 
CCCTCAAATT ATGTGGAGGA CATATCAATG AATGCTTCTG AAATAATTGC AAATCAGCTT 
ATTGAAGACA GTCCATTGGG GTCCATGTGC AGAGGTATCC TGGAAATGGG ATCATATAAT 
ATCAGGAAGT TGCTTAATGG AAAATATAAC AAACAGTTTG TTTTGTCCCT GGAATCCAAG 
AATCCAGAAG AGCCTACTGT AGATTTTGCA GCAGATACAA TAGAAGAGTT TTTTGTGTGG 
TACAAACACA TCCAACAGAT TGAACAGAAA AAGGGAGCAG AGGACTTTGA AAAGGGAACA 
CTGGAGAAGC AGCAAGAGAT TGCCCGAGAA TTATCTGATC TGGTTATGTA CTGTAAGCCC 
AAAAGTGAAG CTAAGGAGAA ATTAGAGTCG CCGGATTTTC GGGAAATTCG ATCATTTGCA 
GAGAATAAGG GAGAGAGTAT TGCTCGACAA AAATCCATTG AGTTGTTGCG GTATAACCGT 
AAAGCCTTTA CGCGAATCTA CCCTAAGTCC TCAAGAGTTG ACTCTTCAAA CTATGACCCC 
CTACCCCTAT GGCTCTGTGG AGACCAGATG ATTGCACTAA ATTACCAGAC TCCAGATAGG 
TACATGCAAA TAAATAATGC AATGTTCGCT TACAATGGAG AATGCGGCTA CATCCTGCAG 
CCAGAGTGTA TGAGGGAAGA GTCATATGAC CCCAGTGACT CTAGAAGACA GCTCCAAATG 
ATTGTGACAA TTAAGGTCTT AGGAGCTCGA CATTTGCCAA AACCAGGGAG AGGTATTGCA 
TGCCCTTTTG TAGAAGTGGA GGTATGCGGT GGGGAATATG ACAACAATAA GTTCAGAACA 
ATTGTTATGA ATGATAATGG CCTCAACCCT GTATGGTCAG GAAGTTCTCA ACAGGCTGAG 
TTTGAGATCC ATGAGCCTGA GATTGCCTTT CTGAGGTTTG TAGTATATGA AGAAGATATG 
TTCAGTGACC CCAACTTCTT AGCTCAGTGC ACCATCCCAG TTAAATGTTT AAAAACAGGT 
TATAGATCAG TTCCACTAAA AAACGGTTAC AGTGAAAACC TAGAGCTTGC TTCTCTGTTG 
GTTTATTGCC AAATACAAAA AGATCTGACT CGCCCACGTG CAAAATTCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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plcg2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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