LOC100496658 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100496658
Entrez Gene ID	100496658
Full Name	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1-like
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031904038.1	XP_031759898.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform X2
XM_018095188.2	XP_017950677.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform X1
XM_031904039.1	XP_031759899.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform X3
XM_018095188.1	XP_017950677.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1-like

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LOC776273	XP_004940140.1
Xenopus tropicalis (tropical clawed frog)	LOC100496658	XP_002943475.2

Gene Ontology

The following LOC100496658 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100496658 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa45278	XM_018095188.2 Latest version!	Xenopus tropicalis 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 (LOC100496658), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa60486	XM_031904038.1 Latest version!	Xenopus tropicalis 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 (LOC100496658), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa60487	XM_031904039.1 Latest version!	Xenopus tropicalis 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 (LOC100496658), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OXa45278	XM_018095188.1	Xenopus tropicalis 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1-like (LOC100496658), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100496658 ( XM_018095188.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950677.1 Xenopus tropicalis 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 (LOC100496658), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa45278
Clone ID Related Accession (Same CDS sequence)	XM_018095188.2 , XM_018095188.1
Accession Version	XM_018095188.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018095188.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGGT GCAGGATGGC CAACCTGAAT GGATTGATGG AGGAGGGCAG GGGGGAGGGC 
ACGGACCTGG GTCGGACCCT GCGGAGCCTG GAGACCGGTA CGGTACTGAC CCTGTTCTAC 
CAAAAGAAGT CACAGCGGCC GGAGAGACGG ACGTTCCAGC TTAGAATGGA CACCAGGCAG 
GTTGTGTGGT TTCGGACCAC GGAGAAGGTG GAAGGAGACA TTGATGTCAG AGAGATTAAG 
GAGATCCGGC CGGGGAAGAA CTCCCGGGAT TTTGAACGCT ACCCTGAAGA TACCCGGAAG 
CTGGACGCTA CCCTCTGCTT CATCATCTTG TATGGAACCG ACTTCCGCCT GAAGACTGTC 
AGTGCTGCTG CTTTCAGTGA GGAAGAGGTA AATCTATGGA TCAGCGGTTT AAACTGGGTC 
GTCGCAGATA CGCTGAAGGC TCCAGGCCCT CTCCAGATCG AGAGGTGGCT CGGAAAACAG 
TTCAACGCAG TGGACAGAGC GAGAGAAGGC AGCGTGACGG TGAAGGATCT GAAAGCTCTG 
CTCCCTCAGG TTAATTACCG CGTGCCTAAC ATGAGATTTC TACGGGACAA AATGCTGGAA 
ATCGAGGCCC GATCGGAGCT GACCTTCCCA CACTTTGTTC TGTTTTACAA TAACTTGATG 
TTTGATGCTC AGAAATCAAT CATTGAGCAG CTCGAGTTGT CTTTCCCTTT AAGGAACGTT 
GATCGGCCTT CTCTATGCCA GATCTCTTTG TATGACTTCC AGAGGTTTCT TCAGTTTGAT 
CAGAAGGAAG CCTGGGCCGG TGACATTAAT AAGGTTCGGG GTTACCTATG CAATTACCTG 
AAGGACAGCG ATGGGGACGT CTGTGAACCC ATTCTGCAGT TGGATGAGTT CCTGACCTAC 
CTCTTCTCCA AGGAGAACAT GATCATGGAC TCTAGGTTTG AGCAAGTGGT GCCGGAGGAG 
ATGACCTACC CGCTGTCCCA GTACTGGATC TCATCCTCAC ACAACACATA TTTGACTGGG 
GATCAGTTCT CCAGCGAGTC ATCCTTAGAA GCTTACGCTC GCTGCCTCCG GATGGGCTGC 
CGCTGCATTG AGTTGGACTG CTGGGATGGA CCAGATGACC TGCCTATTAT CTATCATGGA 
CACACCTTGA CCTCTAAAAT CAAGTTTTTG GATGTACTGC ACACTATCAA GGATAATGCC 
TTTGTCACAT CAGAATATCC GGTTATCTTG TCCATTGAGG ATCACTGCAG TGTTGTCCAG 
CAGAGGAACA TGGCCCATTA TTTCAGAAAG GTATTTGGGG ACATGTTACT GACCAAACCA 
GTGGACATCA ATGCGGACGA GCTCCCCTCT CCGGCCCAGC TCAAAAAGAA GATTCTCATT 
AAGCACAAGA AGCTGGTAGA GGGGAACCTT TATGAGGAGG TCTCAACCGC GAGCTACTCG 
GAGAATGACA TCAGTAACTC TATCAAGAAC GGGATCCTCT ACCTAGAAGA TCCCATAGAC 
CATACCTGGA GCCCTCACTA TTTCGTCTTA ACAAGTAACA AGATCTACTA CTCCGAGGAG 
ACGTCTCGGT ACCAGTCCAA CGATGAGGAC GAAGAGATGG AACCCAAAGA GGAGTTTAAT 
AACAATGAGC TGCACTTCAG TGAGAAGTGG TTCCACGGGA AGCTGGGGGG CGGGCGCGAC 
GGGCGCCAGA TTGCCGAGAA GCTGCTGCAC GAGTACTGCA CGGAGACGGG GGGAAAGGAC 
GGCACATTCC TAGTGCGTGA GAGCGAGACC TTCGTTGGCG ACTACACCTT GTCATTCTGG 
CGCTCCAGCA GGGTCCAGCA CTGCCGCATC CACTCTCGGC AGGAGGCCGG CTCCACCAAA 
TTCTACCTGA CAGACAACCT GGTGTTTGAC AGCCTGTATG GCCTGATCAG CCACTACCGG 
GAGGTGCCGC TGCGCTGCAA TGAGTTTGAG ATGAGGCTCA CCGACCCCGT TCCGCAGCCC 
AACGCACACG AGAGCAAAGA GTGGTACCAT GCCAATCTCA CCCGCCTGCA GGCGGAGCAT 
ATGCTGATGA GAGTTCCCCG AGACGGTGCC TTCCTGTTGC GCAAGCGGAG CGAGACCAGC 
TCATTCGCTA TTTCCTTCAG GGCCGAGGGC AAGATCAAGC ACTGCCGGAT ACAGCAGGAG 
GGCCGACTCT TCATGTTGGG CAGCTCCGCA GAGTTCGAGA GCCTGGTCGA CCTCATTAGT 
TACTATGAGA AGCATCCGCT GTACCGCAAG ATGAGGCTCC GCTATCCCAT CAACGAGGAA 
GCGCTGGAGA AGATGGGCAC GGCGGAACTG GACTACGATG CTCTGTTTGA GGGTAGAACC 
CCCCATGTTT ACGTGGAGGC CAACAAGATG CCCACATCAA GGTGCACGGT GAAAGCGCTG 
TACGATTACA AGGCCCAGCG GGAGGATGAG CTGACGTTCT GCAAGCAAGC CATTATACAC 
AATGTGGACA AACAAGATGG CGGCTGGTGG CGAGGGGATT ACGGAGGCAA AAAGCAACTT 
TGGTTCCCTG CTAATTACGT AGAAGAGACA ACGAGCGCGA TGTCCCCAGA TCAGGATGAG 
GGCTCTTTCT ATTTTGGCCA GTCGGTAGAG AACAGCCCCT TGGGGAACTT CCTGAAAGGA 
TTTATCGATG TGCCTTCCTG CCACATAGTC TCATCGAAGG ACGGAAGGAG CTCGCGGCCG 
TACGTCTTCA CCATCCACTC CCAGCAAATG TCCCACCCCT CTCAGTCCTT GGACGTGGCT 
GCTGACACTC AGGAAGAGCT CAATGATTGG ATCGGCAAAA TCCGAGAGGC CACTCAAAAC 
GCAGATGCTC GGATGCAGGA AGGGAAAATC ATGGAGCGGA GGAAGAAGAT TGCCCTCGAG 
CTGTCGGAGC TTGTTGTCTA TTGCCGGCCA GTTCCGTTCG ATGAGGAAAA GATTGGCACA 
GAGAAGGCTT GCTACCGTGA CATGTCTTCA TTCCCCGAGA CCAAGGCAGA GAAGTACGCC 
AACCGCAGCA AAGGCAAGAA ATTCCTGCAG TACAACCGAC GCCAGCTGAG CAGGGTTTAC 
CCAAAGGGCC AGCGCCTGGA CTCCTCCAAT TATGACCCAC TGCCCATGTG GATCTGTGGC 
AGCCAGCTGG TGGCTCTGAA CTTCCAGACC CCAGACAAGC CGATGCAGCT GAACCAAGCA 
CTGTTCATGC TGGGGGGCCA GAGCGGCTAT GTCCTGCAGC CGGACATCAT GAGAGACGAC 
CTTTTTGATC CCTTCGATAA GAACAGCTTG AAGATTGTGG AGCCAATCAC AGTGCAGCTT 
CAGATATTAG GAGCCAGGCA CCTCCCCAAA AATGGGCGGA GTATCGTTTG CCCCTTCATA 
GAAGTGGAAG TTTGTGGCTC AGAATTCGAC AACAGCAAAA GCAAAAGTGA CGTGGTGGCG 
GATAATGGAC TGAACCCTGT GTGGATGATG AAGCAGTTTG TGTTTGACAT CACCAACCCA 
GAGTTTGCCT TCCTGCGATT CGTGGTGTAC GAGGAGGACA TGTTCAGTGA CCCCAACTTC 
TTGGCCCAAG CCACGTTCTC TGTCAAGGGG CTGAAAACAG GTTACCGCTC CGTGCCGCTG 
AGGAACAGCT ACAGCGAAGA GTTGGAGTTG GCCTCACTGT TGGTGCACAT AGAGATTGTC 
AATGCCAAGG AGGAAGACGA CGAGAATCTC TACACCTCCA TCCAGCAGCT GAGGGACCGG 
GCCAGCGAAT TGTCCAGCCA GGTTTCCAGC TACGAACGGG CCAATAACTG CGACTCTCGC 
TACCAGCAGA GACTGGACGA GCTGCGGGCA GCCCAGGAGC GACTCATGGA ACTTACGGAA 
GTGCGCAACA GGAAGCTGAT GGAGAAGAAG AAGAGAGAGA GACAGTTAGC GAACAAGCGG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950677.1
CDS	906..4811
Translation	MFGCRMANLNGLMEEGRGEGTDLGRTLRSLETGTVLTLFYQKKSQRPERRTFQLRMDTRQVVWFRTTEKVEGDIDVREIKEIRPGKNSRDFERYPEDTRKLDATLCFIILYGTDFRLKTVSAAAFSEEEVNLWISGLNWVVADTLKAPGPLQIERWLGKQFNAVDRAREGSVTVKDLKALLPQVNYRVPNMRFLRDKMLEIEARSELTFPHFVLFYNNLMFDAQKSIIEQLELSFPLRNVDRPSLCQISLYDFQRFLQFDQKEAWAGDINKVRGYLCNYLKDSDGDVCEPILQLDEFLTYLFSKENMIMDSRFEQVVPEEMTYPLSQYWISSSHNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDDLPIIYHGHTLTSKIKFLDVLHTIKDNAFVTSEYPVILSIEDHCSVVQQRNMAHYFRKVFGDMLLTKPVDINADELPSPAQLKKKILIKHKKLVEGNLYEEVSTASYSENDISNSIKNGILYLEDPIDHTWSPHYFVLTSNKIYYSEETSRYQSNDEDEEMEPKEEFNNNELHFSEKWFHGKLGGGRDGRQIAEKLLHEYCTETGGKDGTFLVRESETFVGDYTLSFWRSSRVQHCRIHSRQEAGSTKFYLTDNLVFDSLYGLISHYREVPLRCNEFEMRLTDPVPQPNAHESKEWYHANLTRLQAEHMLMRVPRDGAFLLRKRSETSSFAISFRAEGKIKHCRIQQEGRLFMLGSSAEFESLVDLISYYEKHPLYRKMRLRYPINEEALEKMGTAELDYDALFEGRTPHVYVEANKMPTSRCTVKALYDYKAQREDELTFCKQAIIHNVDKQDGGWWRGDYGGKKQLWFPANYVEETTSAMSPDQDEGSFYFGQSVENSPLGNFLKGFIDVPSCHIVSSKDGRSSRPYVFTIHSQQMSHPSQSLDVAADTQEELNDWIGKIREATQNADARMQEGKIMERRKKIALELSELVVYCRPVPFDEEKIGTEKACYRDMSSFPETKAEKYANRSKGKKFLQYNRRQLSRVYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQLNQALFMLGGQSGYVLQPDIMRDDLFDPFDKNSLKIVEPITVQLQILGARHLPKNGRSIVCPFIEVEVCGSEFDNSKSKSDVVADNGLNPVWMMKQFVFDITNPEFAFLRFVVYEEDMFSDPNFLAQATFSVKGLKTGYRSVPLRNSYSEELELASLLVHIEIVNAKEEDDENLYTSIQQLRDRASELSSQVSSYERANNCDSRYQQRLDELRAAQERLMELTEVRNRKLMEKKKRERQLANKRN

Target ORF information:

RefSeq Version	XM_018095188.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 (LOC100496658), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095188.2

ORF Insert Sequence:

ATGTTTGGGT GCAGGATGGC CAACCTGAAT GGATTGATGG AGGAGGGCAG GGGGGAGGGC 
ACGGACCTGG GTCGGACCCT GCGGAGCCTG GAGACCGGTA CGGTACTGAC CCTGTTCTAC 
CAAAAGAAGT CACAGCGGCC GGAGAGACGG ACGTTCCAGC TTAGAATGGA CACCAGGCAG 
GTTGTGTGGT TTCGGACCAC GGAGAAGGTG GAAGGAGACA TTGATGTCAG AGAGATTAAG 
GAGATCCGGC CGGGGAAGAA CTCCCGGGAT TTTGAACGCT ACCCTGAAGA TACCCGGAAG 
CTGGACGCTA CCCTCTGCTT CATCATCTTG TATGGAACCG ACTTCCGCCT GAAGACTGTC 
AGTGCTGCTG CTTTCAGTGA GGAAGAGGTA AATCTATGGA TCAGCGGTTT AAACTGGGTC 
GTCGCAGATA CGCTGAAGGC TCCAGGCCCT CTCCAGATCG AGAGGTGGCT CGGAAAACAG 
TTCAACGCAG TGGACAGAGC GAGAGAAGGC AGCGTGACGG TGAAGGATCT GAAAGCTCTG 
CTCCCTCAGG TTAATTACCG CGTGCCTAAC ATGAGATTTC TACGGGACAA AATGCTGGAA 
ATCGAGGCCC GATCGGAGCT GACCTTCCCA CACTTTGTTC TGTTTTACAA TAACTTGATG 
TTTGATGCTC AGAAATCAAT CATTGAGCAG CTCGAGTTGT CTTTCCCTTT AAGGAACGTT 
GATCGGCCTT CTCTATGCCA GATCTCTTTG TATGACTTCC AGAGGTTTCT TCAGTTTGAT 
CAGAAGGAAG CCTGGGCCGG TGACATTAAT AAGGTTCGGG GTTACCTATG CAATTACCTG 
AAGGACAGCG ATGGGGACGT CTGTGAACCC ATTCTGCAGT TGGATGAGTT CCTGACCTAC 
CTCTTCTCCA AGGAGAACAT GATCATGGAC TCTAGGTTTG AGCAAGTGGT GCCGGAGGAG 
ATGACCTACC CGCTGTCCCA GTACTGGATC TCATCCTCAC ACAACACATA TTTGACTGGG 
GATCAGTTCT CCAGCGAGTC ATCCTTAGAA GCTTACGCTC GCTGCCTCCG GATGGGCTGC 
CGCTGCATTG AGTTGGACTG CTGGGATGGA CCAGATGACC TGCCTATTAT CTATCATGGA 
CACACCTTGA CCTCTAAAAT CAAGTTTTTG GATGTACTGC ACACTATCAA GGATAATGCC 
TTTGTCACAT CAGAATATCC GGTTATCTTG TCCATTGAGG ATCACTGCAG TGTTGTCCAG 
CAGAGGAACA TGGCCCATTA TTTCAGAAAG GTATTTGGGG ACATGTTACT GACCAAACCA 
GTGGACATCA ATGCGGACGA GCTCCCCTCT CCGGCCCAGC TCAAAAAGAA GATTCTCATT 
AAGCACAAGA AGCTGGTAGA GGGGAACCTT TATGAGGAGG TCTCAACCGC GAGCTACTCG 
GAGAATGACA TCAGTAACTC TATCAAGAAC GGGATCCTCT ACCTAGAAGA TCCCATAGAC 
CATACCTGGA GCCCTCACTA TTTCGTCTTA ACAAGTAACA AGATCTACTA CTCCGAGGAG 
ACGTCTCGGT ACCAGTCCAA CGATGAGGAC GAAGAGATGG AACCCAAAGA GGAGTTTAAT 
AACAATGAGC TGCACTTCAG TGAGAAGTGG TTCCACGGGA AGCTGGGGGG CGGGCGCGAC 
GGGCGCCAGA TTGCCGAGAA GCTGCTGCAC GAGTACTGCA CGGAGACGGG GGGAAAGGAC 
GGCACATTCC TAGTGCGTGA GAGCGAGACC TTCGTTGGCG ACTACACCTT GTCATTCTGG 
CGCTCCAGCA GGGTCCAGCA CTGCCGCATC CACTCTCGGC AGGAGGCCGG CTCCACCAAA 
TTCTACCTGA CAGACAACCT GGTGTTTGAC AGCCTGTATG GCCTGATCAG CCACTACCGG 
GAGGTGCCGC TGCGCTGCAA TGAGTTTGAG ATGAGGCTCA CCGACCCCGT TCCGCAGCCC 
AACGCACACG AGAGCAAAGA GTGGTACCAT GCCAATCTCA CCCGCCTGCA GGCGGAGCAT 
ATGCTGATGA GAGTTCCCCG AGACGGTGCC TTCCTGTTGC GCAAGCGGAG CGAGACCAGC 
TCATTCGCTA TTTCCTTCAG GGCCGAGGGC AAGATCAAGC ACTGCCGGAT ACAGCAGGAG 
GGCCGACTCT TCATGTTGGG CAGCTCCGCA GAGTTCGAGA GCCTGGTCGA CCTCATTAGT 
TACTATGAGA AGCATCCGCT GTACCGCAAG ATGAGGCTCC GCTATCCCAT CAACGAGGAA 
GCGCTGGAGA AGATGGGCAC GGCGGAACTG GACTACGATG CTCTGTTTGA GGGTAGAACC 
CCCCATGTTT ACGTGGAGGC CAACAAGATG CCCACATCAA GGTGCACGGT GAAAGCGCTG 
TACGATTACA AGGCCCAGCG GGAGGATGAG CTGACGTTCT GCAAGCAAGC CATTATACAC 
AATGTGGACA AACAAGATGG CGGCTGGTGG CGAGGGGATT ACGGAGGCAA AAAGCAACTT 
TGGTTCCCTG CTAATTACGT AGAAGAGACA ACGAGCGCGA TGTCCCCAGA TCAGGATGAG 
GGCTCTTTCT ATTTTGGCCA GTCGGTAGAG AACAGCCCCT TGGGGAACTT CCTGAAAGGA 
TTTATCGATG TGCCTTCCTG CCACATAGTC TCATCGAAGG ACGGAAGGAG CTCGCGGCCG 
TACGTCTTCA CCATCCACTC CCAGCAAATG TCCCACCCCT CTCAGTCCTT GGACGTGGCT 
GCTGACACTC AGGAAGAGCT CAATGATTGG ATCGGCAAAA TCCGAGAGGC CACTCAAAAC 
GCAGATGCTC GGATGCAGGA AGGGAAAATC ATGGAGCGGA GGAAGAAGAT TGCCCTCGAG 
CTGTCGGAGC TTGTTGTCTA TTGCCGGCCA GTTCCGTTCG ATGAGGAAAA GATTGGCACA 
GAGAAGGCTT GCTACCGTGA CATGTCTTCA TTCCCCGAGA CCAAGGCAGA GAAGTACGCC 
AACCGCAGCA AAGGCAAGAA ATTCCTGCAG TACAACCGAC GCCAGCTGAG CAGGGTTTAC 
CCAAAGGGCC AGCGCCTGGA CTCCTCCAAT TATGACCCAC TGCCCATGTG GATCTGTGGC 
AGCCAGCTGG TGGCTCTGAA CTTCCAGACC CCAGACAAGC CGATGCAGCT GAACCAAGCA 
CTGTTCATGC TGGGGGGCCA GAGCGGCTAT GTCCTGCAGC CGGACATCAT GAGAGACGAC 
CTTTTTGATC CCTTCGATAA GAACAGCTTG AAGATTGTGG AGCCAATCAC AGTGCAGCTT 
CAGATATTAG GAGCCAGGCA CCTCCCCAAA AATGGGCGGA GTATCGTTTG CCCCTTCATA 
GAAGTGGAAG TTTGTGGCTC AGAATTCGAC AACAGCAAAA GCAAAAGTGA CGTGGTGGCG 
GATAATGGAC TGAACCCTGT GTGGATGATG AAGCAGTTTG TGTTTGACAT CACCAACCCA 
GAGTTTGCCT TCCTGCGATT CGTGGTGTAC GAGGAGGACA TGTTCAGTGA CCCCAACTTC 
TTGGCCCAAG CCACGTTCTC TGTCAAGGGG CTGAAAACAG GTTACCGCTC CGTGCCGCTG 
AGGAACAGCT ACAGCGAAGA GTTGGAGTTG GCCTCACTGT TGGTGCACAT AGAGATTGTC 
AATGCCAAGG AGGAAGACGA CGAGAATCTC TACACCTCCA TCCAGCAGCT GAGGGACCGG 
GCCAGCGAAT TGTCCAGCCA GGTTTCCAGC TACGAACGGG CCAATAACTG CGACTCTCGC 
TACCAGCAGA GACTGGACGA GCTGCGGGCA GCCCAGGAGC GACTCATGGA ACTTACGGAA 
GTGCGCAACA GGAAGCTGAT GGAGAAGAAG AAGAGAGAGA GACAGTTAGC GAACAAGCGG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100496658 ( XM_018095188.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950677.1 Xenopus tropicalis 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1-like (LOC100496658), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa45278
Clone ID Related Accession (Same CDS sequence)	XM_018095188.2 , XM_018095188.1
Accession Version	XM_018095188.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGGT GCAGGATGGC CAACCTGAAT GGATTGATGG AGGAGGGCAG GGGGGAGGGC 
ACGGACCTGG GTCGGACCCT GCGGAGCCTG GAGACCGGTA CGGTACTGAC CCTGTTCTAC 
CAAAAGAAGT CACAGCGGCC GGAGAGACGG ACGTTCCAGC TTAGAATGGA CACCAGGCAG 
GTTGTGTGGT TTCGGACCAC GGAGAAGGTG GAAGGAGACA TTGATGTCAG AGAGATTAAG 
GAGATCCGGC CGGGGAAGAA CTCCCGGGAT TTTGAACGCT ACCCTGAAGA TACCCGGAAG 
CTGGACGCTA CCCTCTGCTT CATCATCTTG TATGGAACCG ACTTCCGCCT GAAGACTGTC 
AGTGCTGCTG CTTTCAGTGA GGAAGAGGTA AATCTATGGA TCAGCGGTTT AAACTGGGTC 
GTCGCAGATA CGCTGAAGGC TCCAGGCCCT CTCCAGATCG AGAGGTGGCT CGGAAAACAG 
TTCAACGCAG TGGACAGAGC GAGAGAAGGC AGCGTGACGG TGAAGGATCT GAAAGCTCTG 
CTCCCTCAGG TTAATTACCG CGTGCCTAAC ATGAGATTTC TACGGGACAA AATGCTGGAA 
ATCGAGGCCC GATCGGAGCT GACCTTCCCA CACTTTGTTC TGTTTTACAA TAACTTGATG 
TTTGATGCTC AGAAATCAAT CATTGAGCAG CTCGAGTTGT CTTTCCCTTT AAGGAACGTT 
GATCGGCCTT CTCTATGCCA GATCTCTTTG TATGACTTCC AGAGGTTTCT TCAGTTTGAT 
CAGAAGGAAG CCTGGGCCGG TGACATTAAT AAGGTTCGGG GTTACCTATG CAATTACCTG 
AAGGACAGCG ATGGGGACGT CTGTGAACCC ATTCTGCAGT TGGATGAGTT CCTGACCTAC 
CTCTTCTCCA AGGAGAACAT GATCATGGAC TCTAGGTTTG AGCAAGTGGT GCCGGAGGAG 
ATGACCTACC CGCTGTCCCA GTACTGGATC TCATCCTCAC ACAACACATA TTTGACTGGG 
GATCAGTTCT CCAGCGAGTC ATCCTTAGAA GCTTACGCTC GCTGCCTCCG GATGGGCTGC 
CGCTGCATTG AGTTGGACTG CTGGGATGGA CCAGATGACC TGCCTATTAT CTATCATGGA 
CACACCTTGA CCTCTAAAAT CAAGTTTTTG GATGTACTGC ACACTATCAA GGATAATGCC 
TTTGTCACAT CAGAATATCC GGTTATCTTG TCCATTGAGG ATCACTGCAG TGTTGTCCAG 
CAGAGGAACA TGGCCCATTA TTTCAGAAAG GTATTTGGGG ACATGTTACT GACCAAACCA 
GTGGACATCA ATGCGGACGA GCTCCCCTCT CCGGCCCAGC TCAAAAAGAA GATTCTCATT 
AAGCACAAGA AGCTGGTAGA GGGGAACCTT TATGAGGAGG TCTCAACCGC GAGCTACTCG 
GAGAATGACA TCAGTAACTC TATCAAGAAC GGGATCCTCT ACCTAGAAGA TCCCATAGAC 
CATACCTGGA GCCCTCACTA TTTCGTCTTA ACAAGTAACA AGATCTACTA CTCCGAGGAG 
ACGTCTCGGT ACCAGTCCAA CGATGAGGAC GAAGAGATGG AACCCAAAGA GGAGTTTAAT 
AACAATGAGC TGCACTTCAG TGAGAAGTGG TTCCACGGGA AGCTGGGGGG CGGGCGCGAC 
GGGCGCCAGA TTGCCGAGAA GCTGCTGCAC GAGTACTGCA CGGAGACGGG GGGAAAGGAC 
GGCACATTCC TAGTGCGTGA GAGCGAGACC TTCGTTGGCG ACTACACCTT GTCATTCTGG 
CGCTCCAGCA GGGTCCAGCA CTGCCGCATC CACTCTCGGC AGGAGGCCGG CTCCACCAAA 
TTCTACCTGA CAGACAACCT GGTGTTTGAC AGCCTGTATG GCCTGATCAG CCACTACCGG 
GAGGTGCCGC TGCGCTGCAA TGAGTTTGAG ATGAGGCTCA CCGACCCCGT TCCGCAGCCC 
AACGCACACG AGAGCAAAGA GTGGTACCAT GCCAATCTCA CCCGCCTGCA GGCGGAGCAT 
ATGCTGATGA GAGTTCCCCG AGACGGTGCC TTCCTGTTGC GCAAGCGGAG CGAGACCAGC 
TCATTCGCTA TTTCCTTCAG GGCCGAGGGC AAGATCAAGC ACTGCCGGAT ACAGCAGGAG 
GGCCGACTCT TCATGTTGGG CAGCTCCGCA GAGTTCGAGA GCCTGGTCGA CCTCATTAGT 
TACTATGAGA AGCATCCGCT GTACCGCAAG ATGAGGCTCC GCTATCCCAT CAACGAGGAA 
GCGCTGGAGA AGATGGGCAC GGCGGAACTG GACTACGATG CTCTGTTTGA GGGTAGAACC 
CCCCATGTTT ACGTGGAGGC CAACAAGATG CCCACATCAA GGTGCACGGT GAAAGCGCTG 
TACGATTACA AGGCCCAGCG GGAGGATGAG CTGACGTTCT GCAAGCAAGC CATTATACAC 
AATGTGGACA AACAAGATGG CGGCTGGTGG CGAGGGGATT ACGGAGGCAA AAAGCAACTT 
TGGTTCCCTG CTAATTACGT AGAAGAGACA ACGAGCGCGA TGTCCCCAGA TCAGGATGAG 
GGCTCTTTCT ATTTTGGCCA GTCGGTAGAG AACAGCCCCT TGGGGAACTT CCTGAAAGGA 
TTTATCGATG TGCCTTCCTG CCACATAGTC TCATCGAAGG ACGGAAGGAG CTCGCGGCCG 
TACGTCTTCA CCATCCACTC CCAGCAAATG TCCCACCCCT CTCAGTCCTT GGACGTGGCT 
GCTGACACTC AGGAAGAGCT CAATGATTGG ATCGGCAAAA TCCGAGAGGC CACTCAAAAC 
GCAGATGCTC GGATGCAGGA AGGGAAAATC ATGGAGCGGA GGAAGAAGAT TGCCCTCGAG 
CTGTCGGAGC TTGTTGTCTA TTGCCGGCCA GTTCCGTTCG ATGAGGAAAA GATTGGCACA 
GAGAAGGCTT GCTACCGTGA CATGTCTTCA TTCCCCGAGA CCAAGGCAGA GAAGTACGCC 
AACCGCAGCA AAGGCAAGAA ATTCCTGCAG TACAACCGAC GCCAGCTGAG CAGGGTTTAC 
CCAAAGGGCC AGCGCCTGGA CTCCTCCAAT TATGACCCAC TGCCCATGTG GATCTGTGGC 
AGCCAGCTGG TGGCTCTGAA CTTCCAGACC CCAGACAAGC CGATGCAGCT GAACCAAGCA 
CTGTTCATGC TGGGGGGCCA GAGCGGCTAT GTCCTGCAGC CGGACATCAT GAGAGACGAC 
CTTTTTGATC CCTTCGATAA GAACAGCTTG AAGATTGTGG AGCCAATCAC AGTGCAGCTT 
CAGATATTAG GAGCCAGGCA CCTCCCCAAA AATGGGCGGA GTATCGTTTG CCCCTTCATA 
GAAGTGGAAG TTTGTGGCTC AGAATTCGAC AACAGCAAAA GCAAAAGTGA CGTGGTGGCG 
GATAATGGAC TGAACCCTGT GTGGATGATG AAGCAGTTTG TGTTTGACAT CACCAACCCA 
GAGTTTGCCT TCCTGCGATT CGTGGTGTAC GAGGAGGACA TGTTCAGTGA CCCCAACTTC 
TTGGCCCAAG CCACGTTCTC TGTCAAGGGG CTGAAAACAG GTTACCGCTC CGTGCCGCTG 
AGGAACAGCT ACAGCGAAGA GTTGGAGTTG GCCTCACTGT TGGTGCACAT AGAGATTGTC 
AATGCCAAGG AGGAAGACGA CGAGAATCTC TACACCTCCA TCCAGCAGCT GAGGGACCGG 
GCCAGCGAAT TGTCCAGCCA GGTTTCCAGC TACGAACGGG CCAATAACTG CGACTCTCGC 
TACCAGCAGA GACTGGACGA GCTGCGGGCA GCCCAGGAGC GACTCATGGA ACTTACGGAA 
GTGCGCAACA GGAAGCTGAT GGAGAAGAAG AAGAGAGAGA GACAGTTAGC GAACAAGCGG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950677.1
CDS	910..4815
Translation	MFGCRMANLNGLMEEGRGEGTDLGRTLRSLETGTVLTLFYQKKSQRPERRTFQLRMDTRQVVWFRTTEKVEGDIDVREIKEIRPGKNSRDFERYPEDTRKLDATLCFIILYGTDFRLKTVSAAAFSEEEVNLWISGLNWVVADTLKAPGPLQIERWLGKQFNAVDRAREGSVTVKDLKALLPQVNYRVPNMRFLRDKMLEIEARSELTFPHFVLFYNNLMFDAQKSIIEQLELSFPLRNVDRPSLCQISLYDFQRFLQFDQKEAWAGDINKVRGYLCNYLKDSDGDVCEPILQLDEFLTYLFSKENMIMDSRFEQVVPEEMTYPLSQYWISSSHNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDDLPIIYHGHTLTSKIKFLDVLHTIKDNAFVTSEYPVILSIEDHCSVVQQRNMAHYFRKVFGDMLLTKPVDINADELPSPAQLKKKILIKHKKLVEGNLYEEVSTASYSENDISNSIKNGILYLEDPIDHTWSPHYFVLTSNKIYYSEETSRYQSNDEDEEMEPKEEFNNNELHFSEKWFHGKLGGGRDGRQIAEKLLHEYCTETGGKDGTFLVRESETFVGDYTLSFWRSSRVQHCRIHSRQEAGSTKFYLTDNLVFDSLYGLISHYREVPLRCNEFEMRLTDPVPQPNAHESKEWYHANLTRLQAEHMLMRVPRDGAFLLRKRSETSSFAISFRAEGKIKHCRIQQEGRLFMLGSSAEFESLVDLISYYEKHPLYRKMRLRYPINEEALEKMGTAELDYDALFEGRTPHVYVEANKMPTSRCTVKALYDYKAQREDELTFCKQAIIHNVDKQDGGWWRGDYGGKKQLWFPANYVEETTSAMSPDQDEGSFYFGQSVENSPLGNFLKGFIDVPSCHIVSSKDGRSSRPYVFTIHSQQMSHPSQSLDVAADTQEELNDWIGKIREATQNADARMQEGKIMERRKKIALELSELVVYCRPVPFDEEKIGTEKACYRDMSSFPETKAEKYANRSKGKKFLQYNRRQLSRVYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQLNQALFMLGGQSGYVLQPDIMRDDLFDPFDKNSLKIVEPITVQLQILGARHLPKNGRSIVCPFIEVEVCGSEFDNSKSKSDVVADNGLNPVWMMKQFVFDITNPEFAFLRFVVYEEDMFSDPNFLAQATFSVKGLKTGYRSVPLRNSYSEELELASLLVHIEIVNAKEEDDENLYTSIQQLRDRASELSSQVSSYERANNCDSRYQQRLDELRAAQERLMELTEVRNRKLMEKKKRERQLANKRN

Target ORF information:

RefSeq Version	XM_018095188.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1-like (LOC100496658), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095188.1

ORF Insert Sequence:

ATGTTTGGGT GCAGGATGGC CAACCTGAAT GGATTGATGG AGGAGGGCAG GGGGGAGGGC 
ACGGACCTGG GTCGGACCCT GCGGAGCCTG GAGACCGGTA CGGTACTGAC CCTGTTCTAC 
CAAAAGAAGT CACAGCGGCC GGAGAGACGG ACGTTCCAGC TTAGAATGGA CACCAGGCAG 
GTTGTGTGGT TTCGGACCAC GGAGAAGGTG GAAGGAGACA TTGATGTCAG AGAGATTAAG 
GAGATCCGGC CGGGGAAGAA CTCCCGGGAT TTTGAACGCT ACCCTGAAGA TACCCGGAAG 
CTGGACGCTA CCCTCTGCTT CATCATCTTG TATGGAACCG ACTTCCGCCT GAAGACTGTC 
AGTGCTGCTG CTTTCAGTGA GGAAGAGGTA AATCTATGGA TCAGCGGTTT AAACTGGGTC 
GTCGCAGATA CGCTGAAGGC TCCAGGCCCT CTCCAGATCG AGAGGTGGCT CGGAAAACAG 
TTCAACGCAG TGGACAGAGC GAGAGAAGGC AGCGTGACGG TGAAGGATCT GAAAGCTCTG 
CTCCCTCAGG TTAATTACCG CGTGCCTAAC ATGAGATTTC TACGGGACAA AATGCTGGAA 
ATCGAGGCCC GATCGGAGCT GACCTTCCCA CACTTTGTTC TGTTTTACAA TAACTTGATG 
TTTGATGCTC AGAAATCAAT CATTGAGCAG CTCGAGTTGT CTTTCCCTTT AAGGAACGTT 
GATCGGCCTT CTCTATGCCA GATCTCTTTG TATGACTTCC AGAGGTTTCT TCAGTTTGAT 
CAGAAGGAAG CCTGGGCCGG TGACATTAAT AAGGTTCGGG GTTACCTATG CAATTACCTG 
AAGGACAGCG ATGGGGACGT CTGTGAACCC ATTCTGCAGT TGGATGAGTT CCTGACCTAC 
CTCTTCTCCA AGGAGAACAT GATCATGGAC TCTAGGTTTG AGCAAGTGGT GCCGGAGGAG 
ATGACCTACC CGCTGTCCCA GTACTGGATC TCATCCTCAC ACAACACATA TTTGACTGGG 
GATCAGTTCT CCAGCGAGTC ATCCTTAGAA GCTTACGCTC GCTGCCTCCG GATGGGCTGC 
CGCTGCATTG AGTTGGACTG CTGGGATGGA CCAGATGACC TGCCTATTAT CTATCATGGA 
CACACCTTGA CCTCTAAAAT CAAGTTTTTG GATGTACTGC ACACTATCAA GGATAATGCC 
TTTGTCACAT CAGAATATCC GGTTATCTTG TCCATTGAGG ATCACTGCAG TGTTGTCCAG 
CAGAGGAACA TGGCCCATTA TTTCAGAAAG GTATTTGGGG ACATGTTACT GACCAAACCA 
GTGGACATCA ATGCGGACGA GCTCCCCTCT CCGGCCCAGC TCAAAAAGAA GATTCTCATT 
AAGCACAAGA AGCTGGTAGA GGGGAACCTT TATGAGGAGG TCTCAACCGC GAGCTACTCG 
GAGAATGACA TCAGTAACTC TATCAAGAAC GGGATCCTCT ACCTAGAAGA TCCCATAGAC 
CATACCTGGA GCCCTCACTA TTTCGTCTTA ACAAGTAACA AGATCTACTA CTCCGAGGAG 
ACGTCTCGGT ACCAGTCCAA CGATGAGGAC GAAGAGATGG AACCCAAAGA GGAGTTTAAT 
AACAATGAGC TGCACTTCAG TGAGAAGTGG TTCCACGGGA AGCTGGGGGG CGGGCGCGAC 
GGGCGCCAGA TTGCCGAGAA GCTGCTGCAC GAGTACTGCA CGGAGACGGG GGGAAAGGAC 
GGCACATTCC TAGTGCGTGA GAGCGAGACC TTCGTTGGCG ACTACACCTT GTCATTCTGG 
CGCTCCAGCA GGGTCCAGCA CTGCCGCATC CACTCTCGGC AGGAGGCCGG CTCCACCAAA 
TTCTACCTGA CAGACAACCT GGTGTTTGAC AGCCTGTATG GCCTGATCAG CCACTACCGG 
GAGGTGCCGC TGCGCTGCAA TGAGTTTGAG ATGAGGCTCA CCGACCCCGT TCCGCAGCCC 
AACGCACACG AGAGCAAAGA GTGGTACCAT GCCAATCTCA CCCGCCTGCA GGCGGAGCAT 
ATGCTGATGA GAGTTCCCCG AGACGGTGCC TTCCTGTTGC GCAAGCGGAG CGAGACCAGC 
TCATTCGCTA TTTCCTTCAG GGCCGAGGGC AAGATCAAGC ACTGCCGGAT ACAGCAGGAG 
GGCCGACTCT TCATGTTGGG CAGCTCCGCA GAGTTCGAGA GCCTGGTCGA CCTCATTAGT 
TACTATGAGA AGCATCCGCT GTACCGCAAG ATGAGGCTCC GCTATCCCAT CAACGAGGAA 
GCGCTGGAGA AGATGGGCAC GGCGGAACTG GACTACGATG CTCTGTTTGA GGGTAGAACC 
CCCCATGTTT ACGTGGAGGC CAACAAGATG CCCACATCAA GGTGCACGGT GAAAGCGCTG 
TACGATTACA AGGCCCAGCG GGAGGATGAG CTGACGTTCT GCAAGCAAGC CATTATACAC 
AATGTGGACA AACAAGATGG CGGCTGGTGG CGAGGGGATT ACGGAGGCAA AAAGCAACTT 
TGGTTCCCTG CTAATTACGT AGAAGAGACA ACGAGCGCGA TGTCCCCAGA TCAGGATGAG 
GGCTCTTTCT ATTTTGGCCA GTCGGTAGAG AACAGCCCCT TGGGGAACTT CCTGAAAGGA 
TTTATCGATG TGCCTTCCTG CCACATAGTC TCATCGAAGG ACGGAAGGAG CTCGCGGCCG 
TACGTCTTCA CCATCCACTC CCAGCAAATG TCCCACCCCT CTCAGTCCTT GGACGTGGCT 
GCTGACACTC AGGAAGAGCT CAATGATTGG ATCGGCAAAA TCCGAGAGGC CACTCAAAAC 
GCAGATGCTC GGATGCAGGA AGGGAAAATC ATGGAGCGGA GGAAGAAGAT TGCCCTCGAG 
CTGTCGGAGC TTGTTGTCTA TTGCCGGCCA GTTCCGTTCG ATGAGGAAAA GATTGGCACA 
GAGAAGGCTT GCTACCGTGA CATGTCTTCA TTCCCCGAGA CCAAGGCAGA GAAGTACGCC 
AACCGCAGCA AAGGCAAGAA ATTCCTGCAG TACAACCGAC GCCAGCTGAG CAGGGTTTAC 
CCAAAGGGCC AGCGCCTGGA CTCCTCCAAT TATGACCCAC TGCCCATGTG GATCTGTGGC 
AGCCAGCTGG TGGCTCTGAA CTTCCAGACC CCAGACAAGC CGATGCAGCT GAACCAAGCA 
CTGTTCATGC TGGGGGGCCA GAGCGGCTAT GTCCTGCAGC CGGACATCAT GAGAGACGAC 
CTTTTTGATC CCTTCGATAA GAACAGCTTG AAGATTGTGG AGCCAATCAC AGTGCAGCTT 
CAGATATTAG GAGCCAGGCA CCTCCCCAAA AATGGGCGGA GTATCGTTTG CCCCTTCATA 
GAAGTGGAAG TTTGTGGCTC AGAATTCGAC AACAGCAAAA GCAAAAGTGA CGTGGTGGCG 
GATAATGGAC TGAACCCTGT GTGGATGATG AAGCAGTTTG TGTTTGACAT CACCAACCCA 
GAGTTTGCCT TCCTGCGATT CGTGGTGTAC GAGGAGGACA TGTTCAGTGA CCCCAACTTC 
TTGGCCCAAG CCACGTTCTC TGTCAAGGGG CTGAAAACAG GTTACCGCTC CGTGCCGCTG 
AGGAACAGCT ACAGCGAAGA GTTGGAGTTG GCCTCACTGT TGGTGCACAT AGAGATTGTC 
AATGCCAAGG AGGAAGACGA CGAGAATCTC TACACCTCCA TCCAGCAGCT GAGGGACCGG 
GCCAGCGAAT TGTCCAGCCA GGTTTCCAGC TACGAACGGG CCAATAACTG CGACTCTCGC 
TACCAGCAGA GACTGGACGA GCTGCGGGCA GCCCAGGAGC GACTCATGGA ACTTACGGAA 
GTGCGCAACA GGAAGCTGAT GGAGAAGAAG AAGAGAGAGA GACAGTTAGC GAACAAGCGG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100496658 ( XM_031904038.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759898.1 Xenopus tropicalis 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 (LOC100496658), transcript variant X2, mRNA.

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CloneID	OXa60486
Clone ID Related Accession (Same CDS sequence)	XM_031904038.1
Accession Version	XM_031904038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAC CCTCAGTGTG TCTGTGTGTG GGGCCCCCCT CTGCTAAGTA CCTGCTCCCT 
GCCAGCAACT TAGTCGTGAC GGTGAAGGAT CTGAAAGCTC TGCTCCCTCA GGTTAATTAC 
CGCGTGCCTA ACATGAGATT TCTACGGGAC AAAATGCTGG AAATCGAGGC CCGATCGGAG 
CTGACCTTCC CACACTTTGT TCTGTTTTAC AATAACTTGA TGTTTGATGC TCAGAAATCA 
ATCATTGAGC AGCTCGAGTT GTCTTTCCCT TTAAGGAACG TTGATCGGCC TTCTCTATGC 
CAGATCTCTT TGTATGACTT CCAGAGGTTT CTTCAGTTTG ATCAGAAGGA AGCCTGGGCC 
GGTGACATTA ATAAGGTTCG GGGTTACCTA TGCAATTACC TGAAGGACAG CGATGGGGAC 
GTCTGTGAAC CCATTCTGCA GTTGGATGAG TTCCTGACCT ACCTCTTCTC CAAGGAGAAC 
ATGATCATGG ACTCTAGGTT TGAGCAAGTG GTGCCGGAGG AGATGACCTA CCCGCTGTCC 
CAGTACTGGA TCTCATCCTC ACACAACACA TATTTGACTG GGGATCAGTT CTCCAGCGAG 
TCATCCTTAG AAGCTTACGC TCGCTGCCTC CGGATGGGCT GCCGCTGCAT TGAGTTGGAC 
TGCTGGGATG GACCAGATGA CCTGCCTATT ATCTATCATG GACACACCTT GACCTCTAAA 
ATCAAGTTTT TGGATGTACT GCACACTATC AAGGATAATG CCTTTGTCAC ATCAGAATAT 
CCGGTTATCT TGTCCATTGA GGATCACTGC AGTGTTGTCC AGCAGAGGAA CATGGCCCAT 
TATTTCAGAA AGGTATTTGG GGACATGTTA CTGACCAAAC CAGTGGACAT CAATGCGGAC 
GAGCTCCCCT CTCCGGCCCA GCTCAAAAAG AAGATTCTCA TTAAGCACAA GAAGCTGGTA 
GAGGGGAACC TTTATGAGGA GGTCTCAACC GCGAGCTACT CGGAGAATGA CATCAGTAAC 
TCTATCAAGA ACGGGATCCT CTACCTAGAA GATCCCATAG ACCATACCTG GAGCCCTCAC 
TATTTCGTCT TAACAAGTAA CAAGATCTAC TACTCCGAGG AGACGTCTCG GTACCAGTCC 
AACGATGAGG ACGAAGAGAT GGAACCCAAA GAGGAGTTTA ATAACAATGA GCTGCACTTC 
AGTGAGAAGT GGTTCCACGG GAAGCTGGGG GGCGGGCGCG ACGGGCGCCA GATTGCCGAG 
AAGCTGCTGC ACGAGTACTG CACGGAGACG GGGGGAAAGG ACGGCACATT CCTAGTGCGT 
GAGAGCGAGA CCTTCGTTGG CGACTACACC TTGTCATTCT GGCGCTCCAG CAGGGTCCAG 
CACTGCCGCA TCCACTCTCG GCAGGAGGCC GGCTCCACCA AATTCTACCT GACAGACAAC 
CTGGTGTTTG ACAGCCTGTA TGGCCTGATC AGCCACTACC GGGAGGTGCC GCTGCGCTGC 
AATGAGTTTG AGATGAGGCT CACCGACCCC GTTCCGCAGC CCAACGCACA CGAGAGCAAA 
GAGTGGTACC ATGCCAATCT CACCCGCCTG CAGGCGGAGC ATATGCTGAT GAGAGTTCCC 
CGAGACGGTG CCTTCCTGTT GCGCAAGCGG AGCGAGACCA GCTCATTCGC TATTTCCTTC 
AGGGCCGAGG GCAAGATCAA GCACTGCCGG ATACAGCAGG AGGGCCGACT CTTCATGTTG 
GGCAGCTCCG CAGAGTTCGA GAGCCTGGTC GACCTCATTA GTTACTATGA GAAGCATCCG 
CTGTACCGCA AGATGAGGCT CCGCTATCCC ATCAACGAGG AAGCGCTGGA GAAGATGGGC 
ACGGCGGAAC TGGACTACGA TGCTCTGTTT GAGGGTAGAA CCCCCCATGT TTACGTGGAG 
GCCAACAAGA TGCCCACATC AAGGTGCACG GTGAAAGCGC TGTACGATTA CAAGGCCCAG 
CGGGAGGATG AGCTGACGTT CTGCAAGCAA GCCATTATAC ACAATGTGGA CAAACAAGAT 
GGCGGCTGGT GGCGAGGGGA TTACGGAGGC AAAAAGCAAC TTTGGTTCCC TGCTAATTAC 
GTAGAAGAGA CAACGAGCGC GATGTCCCCA GATCAGGATG AGGGCTCTTT CTATTTTGGC 
CAGTCGGTAG AGAACAGCCC CTTGGGGAAC TTCCTGAAAG GATTTATCGA TGTGCCTTCC 
TGCCACATAG TCTCATCGAA GGACGGAAGG AGCTCGCGGC CGTACGTCTT CACCATCCAC 
TCCCAGCAAA TGTCCCACCC CTCTCAGTCC TTGGACGTGG CTGCTGACAC TCAGGAAGAG 
CTCAATGATT GGATCGGCAA AATCCGAGAG GCCACTCAAA ACGCAGATGC TCGGATGCAG 
GAAGGGAAAA TCATGGAGCG GAGGAAGAAG ATTGCCCTCG AGCTGTCGGA GCTTGTTGTC 
TATTGCCGGC CAGTTCCGTT CGATGAGGAA AAGATTGGCA CAGAGAAGGC TTGCTACCGT 
GACATGTCTT CATTCCCCGA GACCAAGGCA GAGAAGTACG CCAACCGCAG CAAAGGCAAG 
AAATTCCTGC AGTACAACCG ACGCCAGCTG AGCAGGGTTT ACCCAAAGGG CCAGCGCCTG 
GACTCCTCCA ATTATGACCC ACTGCCCATG TGGATCTGTG GCAGCCAGCT GGTGGCTCTG 
AACTTCCAGA CCCCAGACAA GCCGATGCAG CTGAACCAAG CACTGTTCAT GCTGGGGGGC 
CAGAGCGGCT ATGTCCTGCA GCCGGACATC ATGAGAGACG ACCTTTTTGA TCCCTTCGAT 
AAGAACAGCT TGAAGATTGT GGAGCCAATC ACAGTGCAGC TTCAGATATT AGGAGCCAGG 
CACCTCCCCA AAAATGGGCG GAGTATCGTT TGCCCCTTCA TAGAAGTGGA AGTTTGTGGC 
TCAGAATTCG ACAACAGCAA AAGCAAAAGT GACGTGGTGG CGGATAATGG ACTGAACCCT 
GTGTGGATGA TGAAGCAGTT TGTGTTTGAC ATCACCAACC CAGAGTTTGC CTTCCTGCGA 
TTCGTGGTGT ACGAGGAGGA CATGTTCAGT GACCCCAACT TCTTGGCCCA AGCCACGTTC 
TCTGTCAAGG GGCTGAAAAC AGGTTACCGC TCCGTGCCGC TGAGGAACAG CTACAGCGAA 
GAGTTGGAGT TGGCCTCACT GTTGGTGCAC ATAGAGATTG TCAATGCCAA GGAGGAAGAC 
GACGAGAATC TCTACACCTC CATCCAGCAG CTGAGGGACC GGGCCAGCGA ATTGTCCAGC 
CAGGTTTCCA GCTACGAACG GGCCAATAAC TGCGACTCTC GCTACCAGCA GAGACTGGAC 
GAGCTGCGGG CAGCCCAGGA GCGACTCATG GAACTTACGG AAGTGCGCAA CAGGAAGCTG 
ATGGAGAAGA AGAAGAGAGA GAGACAGTTA GCGAACAAGC GGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759898.1
CDS	16..3483
Translation	MEPPSVCLCVGPPSAKYLLPASNLVVTVKDLKALLPQVNYRVPNMRFLRDKMLEIEARSELTFPHFVLFYNNLMFDAQKSIIEQLELSFPLRNVDRPSLCQISLYDFQRFLQFDQKEAWAGDINKVRGYLCNYLKDSDGDVCEPILQLDEFLTYLFSKENMIMDSRFEQVVPEEMTYPLSQYWISSSHNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDDLPIIYHGHTLTSKIKFLDVLHTIKDNAFVTSEYPVILSIEDHCSVVQQRNMAHYFRKVFGDMLLTKPVDINADELPSPAQLKKKILIKHKKLVEGNLYEEVSTASYSENDISNSIKNGILYLEDPIDHTWSPHYFVLTSNKIYYSEETSRYQSNDEDEEMEPKEEFNNNELHFSEKWFHGKLGGGRDGRQIAEKLLHEYCTETGGKDGTFLVRESETFVGDYTLSFWRSSRVQHCRIHSRQEAGSTKFYLTDNLVFDSLYGLISHYREVPLRCNEFEMRLTDPVPQPNAHESKEWYHANLTRLQAEHMLMRVPRDGAFLLRKRSETSSFAISFRAEGKIKHCRIQQEGRLFMLGSSAEFESLVDLISYYEKHPLYRKMRLRYPINEEALEKMGTAELDYDALFEGRTPHVYVEANKMPTSRCTVKALYDYKAQREDELTFCKQAIIHNVDKQDGGWWRGDYGGKKQLWFPANYVEETTSAMSPDQDEGSFYFGQSVENSPLGNFLKGFIDVPSCHIVSSKDGRSSRPYVFTIHSQQMSHPSQSLDVAADTQEELNDWIGKIREATQNADARMQEGKIMERRKKIALELSELVVYCRPVPFDEEKIGTEKACYRDMSSFPETKAEKYANRSKGKKFLQYNRRQLSRVYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQLNQALFMLGGQSGYVLQPDIMRDDLFDPFDKNSLKIVEPITVQLQILGARHLPKNGRSIVCPFIEVEVCGSEFDNSKSKSDVVADNGLNPVWMMKQFVFDITNPEFAFLRFVVYEEDMFSDPNFLAQATFSVKGLKTGYRSVPLRNSYSEELELASLLVHIEIVNAKEEDDENLYTSIQQLRDRASELSSQVSSYERANNCDSRYQQRLDELRAAQERLMELTEVRNRKLMEKKKRERQLANKRN

Target ORF information:

RefSeq Version	XM_031904038.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 (LOC100496658), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031904038.1

ORF Insert Sequence:

ATGGAGCCAC CCTCAGTGTG TCTGTGTGTG GGGCCCCCCT CTGCTAAGTA CCTGCTCCCT 
GCCAGCAACT TAGTCGTGAC GGTGAAGGAT CTGAAAGCTC TGCTCCCTCA GGTTAATTAC 
CGCGTGCCTA ACATGAGATT TCTACGGGAC AAAATGCTGG AAATCGAGGC CCGATCGGAG 
CTGACCTTCC CACACTTTGT TCTGTTTTAC AATAACTTGA TGTTTGATGC TCAGAAATCA 
ATCATTGAGC AGCTCGAGTT GTCTTTCCCT TTAAGGAACG TTGATCGGCC TTCTCTATGC 
CAGATCTCTT TGTATGACTT CCAGAGGTTT CTTCAGTTTG ATCAGAAGGA AGCCTGGGCC 
GGTGACATTA ATAAGGTTCG GGGTTACCTA TGCAATTACC TGAAGGACAG CGATGGGGAC 
GTCTGTGAAC CCATTCTGCA GTTGGATGAG TTCCTGACCT ACCTCTTCTC CAAGGAGAAC 
ATGATCATGG ACTCTAGGTT TGAGCAAGTG GTGCCGGAGG AGATGACCTA CCCGCTGTCC 
CAGTACTGGA TCTCATCCTC ACACAACACA TATTTGACTG GGGATCAGTT CTCCAGCGAG 
TCATCCTTAG AAGCTTACGC TCGCTGCCTC CGGATGGGCT GCCGCTGCAT TGAGTTGGAC 
TGCTGGGATG GACCAGATGA CCTGCCTATT ATCTATCATG GACACACCTT GACCTCTAAA 
ATCAAGTTTT TGGATGTACT GCACACTATC AAGGATAATG CCTTTGTCAC ATCAGAATAT 
CCGGTTATCT TGTCCATTGA GGATCACTGC AGTGTTGTCC AGCAGAGGAA CATGGCCCAT 
TATTTCAGAA AGGTATTTGG GGACATGTTA CTGACCAAAC CAGTGGACAT CAATGCGGAC 
GAGCTCCCCT CTCCGGCCCA GCTCAAAAAG AAGATTCTCA TTAAGCACAA GAAGCTGGTA 
GAGGGGAACC TTTATGAGGA GGTCTCAACC GCGAGCTACT CGGAGAATGA CATCAGTAAC 
TCTATCAAGA ACGGGATCCT CTACCTAGAA GATCCCATAG ACCATACCTG GAGCCCTCAC 
TATTTCGTCT TAACAAGTAA CAAGATCTAC TACTCCGAGG AGACGTCTCG GTACCAGTCC 
AACGATGAGG ACGAAGAGAT GGAACCCAAA GAGGAGTTTA ATAACAATGA GCTGCACTTC 
AGTGAGAAGT GGTTCCACGG GAAGCTGGGG GGCGGGCGCG ACGGGCGCCA GATTGCCGAG 
AAGCTGCTGC ACGAGTACTG CACGGAGACG GGGGGAAAGG ACGGCACATT CCTAGTGCGT 
GAGAGCGAGA CCTTCGTTGG CGACTACACC TTGTCATTCT GGCGCTCCAG CAGGGTCCAG 
CACTGCCGCA TCCACTCTCG GCAGGAGGCC GGCTCCACCA AATTCTACCT GACAGACAAC 
CTGGTGTTTG ACAGCCTGTA TGGCCTGATC AGCCACTACC GGGAGGTGCC GCTGCGCTGC 
AATGAGTTTG AGATGAGGCT CACCGACCCC GTTCCGCAGC CCAACGCACA CGAGAGCAAA 
GAGTGGTACC ATGCCAATCT CACCCGCCTG CAGGCGGAGC ATATGCTGAT GAGAGTTCCC 
CGAGACGGTG CCTTCCTGTT GCGCAAGCGG AGCGAGACCA GCTCATTCGC TATTTCCTTC 
AGGGCCGAGG GCAAGATCAA GCACTGCCGG ATACAGCAGG AGGGCCGACT CTTCATGTTG 
GGCAGCTCCG CAGAGTTCGA GAGCCTGGTC GACCTCATTA GTTACTATGA GAAGCATCCG 
CTGTACCGCA AGATGAGGCT CCGCTATCCC ATCAACGAGG AAGCGCTGGA GAAGATGGGC 
ACGGCGGAAC TGGACTACGA TGCTCTGTTT GAGGGTAGAA CCCCCCATGT TTACGTGGAG 
GCCAACAAGA TGCCCACATC AAGGTGCACG GTGAAAGCGC TGTACGATTA CAAGGCCCAG 
CGGGAGGATG AGCTGACGTT CTGCAAGCAA GCCATTATAC ACAATGTGGA CAAACAAGAT 
GGCGGCTGGT GGCGAGGGGA TTACGGAGGC AAAAAGCAAC TTTGGTTCCC TGCTAATTAC 
GTAGAAGAGA CAACGAGCGC GATGTCCCCA GATCAGGATG AGGGCTCTTT CTATTTTGGC 
CAGTCGGTAG AGAACAGCCC CTTGGGGAAC TTCCTGAAAG GATTTATCGA TGTGCCTTCC 
TGCCACATAG TCTCATCGAA GGACGGAAGG AGCTCGCGGC CGTACGTCTT CACCATCCAC 
TCCCAGCAAA TGTCCCACCC CTCTCAGTCC TTGGACGTGG CTGCTGACAC TCAGGAAGAG 
CTCAATGATT GGATCGGCAA AATCCGAGAG GCCACTCAAA ACGCAGATGC TCGGATGCAG 
GAAGGGAAAA TCATGGAGCG GAGGAAGAAG ATTGCCCTCG AGCTGTCGGA GCTTGTTGTC 
TATTGCCGGC CAGTTCCGTT CGATGAGGAA AAGATTGGCA CAGAGAAGGC TTGCTACCGT 
GACATGTCTT CATTCCCCGA GACCAAGGCA GAGAAGTACG CCAACCGCAG CAAAGGCAAG 
AAATTCCTGC AGTACAACCG ACGCCAGCTG AGCAGGGTTT ACCCAAAGGG CCAGCGCCTG 
GACTCCTCCA ATTATGACCC ACTGCCCATG TGGATCTGTG GCAGCCAGCT GGTGGCTCTG 
AACTTCCAGA CCCCAGACAA GCCGATGCAG CTGAACCAAG CACTGTTCAT GCTGGGGGGC 
CAGAGCGGCT ATGTCCTGCA GCCGGACATC ATGAGAGACG ACCTTTTTGA TCCCTTCGAT 
AAGAACAGCT TGAAGATTGT GGAGCCAATC ACAGTGCAGC TTCAGATATT AGGAGCCAGG 
CACCTCCCCA AAAATGGGCG GAGTATCGTT TGCCCCTTCA TAGAAGTGGA AGTTTGTGGC 
TCAGAATTCG ACAACAGCAA AAGCAAAAGT GACGTGGTGG CGGATAATGG ACTGAACCCT 
GTGTGGATGA TGAAGCAGTT TGTGTTTGAC ATCACCAACC CAGAGTTTGC CTTCCTGCGA 
TTCGTGGTGT ACGAGGAGGA CATGTTCAGT GACCCCAACT TCTTGGCCCA AGCCACGTTC 
TCTGTCAAGG GGCTGAAAAC AGGTTACCGC TCCGTGCCGC TGAGGAACAG CTACAGCGAA 
GAGTTGGAGT TGGCCTCACT GTTGGTGCAC ATAGAGATTG TCAATGCCAA GGAGGAAGAC 
GACGAGAATC TCTACACCTC CATCCAGCAG CTGAGGGACC GGGCCAGCGA ATTGTCCAGC 
CAGGTTTCCA GCTACGAACG GGCCAATAAC TGCGACTCTC GCTACCAGCA GAGACTGGAC 
GAGCTGCGGG CAGCCCAGGA GCGACTCATG GAACTTACGG AAGTGCGCAA CAGGAAGCTG 
ATGGAGAAGA AGAAGAGAGA GAGACAGTTA GCGAACAAGC GGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100496658 ( XM_031904039.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759899.1 Xenopus tropicalis 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 (LOC100496658), transcript variant X3, mRNA.

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CloneID	OXa60487
Clone ID Related Accession (Same CDS sequence)	XM_031904039.1
Accession Version	XM_031904039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTC TACGGGACAA AATGCTGGAA ATCGAGGCCC GATCGGAGCT GACCTTCCCA 
CACTTTGTTC TGTTTTACAA TAACTTGATG TTTGATGCTC AGAAATCAAT CATTGAGCAG 
CTCGAGTTGT CTTTCCCTTT AAGGAACGTT GATCGGCCTT CTCTATGCCA GATCTCTTTG 
TATGACTTCC AGAGGTTTCT TCAGTTTGAT CAGAAGGAAG CCTGGGCCGG TGACATTAAT 
AAGGTTCGGG GTTACCTATG CAATTACCTG AAGGACAGCG ATGGGGACGT CTGTGAACCC 
ATTCTGCAGT TGGATGAGTT CCTGACCTAC CTCTTCTCCA AGGAGAACAT GATCATGGAC 
TCTAGGTTTG AGCAAGTGGT GCCGGAGGAG ATGACCTACC CGCTGTCCCA GTACTGGATC 
TCATCCTCAC ACAACACATA TTTGACTGGG GATCAGTTCT CCAGCGAGTC ATCCTTAGAA 
GCTTACGCTC GCTGCCTCCG GATGGGCTGC CGCTGCATTG AGTTGGACTG CTGGGATGGA 
CCAGATGACC TGCCTATTAT CTATCATGGA CACACCTTGA CCTCTAAAAT CAAGTTTTTG 
GATGTACTGC ACACTATCAA GGATAATGCC TTTGTCACAT CAGAATATCC GGTTATCTTG 
TCCATTGAGG ATCACTGCAG TGTTGTCCAG CAGAGGAACA TGGCCCATTA TTTCAGAAAG 
GTATTTGGGG ACATGTTACT GACCAAACCA GTGGACATCA ATGCGGACGA GCTCCCCTCT 
CCGGCCCAGC TCAAAAAGAA GATTCTCATT AAGCACAAGA AGCTGGTAGA GGGGAACCTT 
TATGAGGAGG TCTCAACCGC GAGCTACTCG GAGAATGACA TCAGTAACTC TATCAAGAAC 
GGGATCCTCT ACCTAGAAGA TCCCATAGAC CATACCTGGA GCCCTCACTA TTTCGTCTTA 
ACAAGTAACA AGATCTACTA CTCCGAGGAG ACGTCTCGGT ACCAGTCCAA CGATGAGGAC 
GAAGAGATGG AACCCAAAGA GGAGTTTAAT AACAATGAGC TGCACTTCAG TGAGAAGTGG 
TTCCACGGGA AGCTGGGGGG CGGGCGCGAC GGGCGCCAGA TTGCCGAGAA GCTGCTGCAC 
GAGTACTGCA CGGAGACGGG GGGAAAGGAC GGCACATTCC TAGTGCGTGA GAGCGAGACC 
TTCGTTGGCG ACTACACCTT GTCATTCTGG CGCTCCAGCA GGGTCCAGCA CTGCCGCATC 
CACTCTCGGC AGGAGGCCGG CTCCACCAAA TTCTACCTGA CAGACAACCT GGTGTTTGAC 
AGCCTGTATG GCCTGATCAG CCACTACCGG GAGGTGCCGC TGCGCTGCAA TGAGTTTGAG 
ATGAGGCTCA CCGACCCCGT TCCGCAGCCC AACGCACACG AGAGCAAAGA GTGGTACCAT 
GCCAATCTCA CCCGCCTGCA GGCGGAGCAT ATGCTGATGA GAGTTCCCCG AGACGGTGCC 
TTCCTGTTGC GCAAGCGGAG CGAGACCAGC TCATTCGCTA TTTCCTTCAG GGCCGAGGGC 
AAGATCAAGC ACTGCCGGAT ACAGCAGGAG GGCCGACTCT TCATGTTGGG CAGCTCCGCA 
GAGTTCGAGA GCCTGGTCGA CCTCATTAGT TACTATGAGA AGCATCCGCT GTACCGCAAG 
ATGAGGCTCC GCTATCCCAT CAACGAGGAA GCGCTGGAGA AGATGGGCAC GGCGGAACTG 
GACTACGATG CTCTGTTTGA GGGTAGAACC CCCCATGTTT ACGTGGAGGC CAACAAGATG 
CCCACATCAA GGTGCACGGT GAAAGCGCTG TACGATTACA AGGCCCAGCG GGAGGATGAG 
CTGACGTTCT GCAAGCAAGC CATTATACAC AATGTGGACA AACAAGATGG CGGCTGGTGG 
CGAGGGGATT ACGGAGGCAA AAAGCAACTT TGGTTCCCTG CTAATTACGT AGAAGAGACA 
ACGAGCGCGA TGTCCCCAGA TCAGGATGAG GGCTCTTTCT ATTTTGGCCA GTCGGTAGAG 
AACAGCCCCT TGGGGAACTT CCTGAAAGGA TTTATCGATG TGCCTTCCTG CCACATAGTC 
TCATCGAAGG ACGGAAGGAG CTCGCGGCCG TACGTCTTCA CCATCCACTC CCAGCAAATG 
TCCCACCCCT CTCAGTCCTT GGACGTGGCT GCTGACACTC AGGAAGAGCT CAATGATTGG 
ATCGGCAAAA TCCGAGAGGC CACTCAAAAC GCAGATGCTC GGATGCAGGA AGGGAAAATC 
ATGGAGCGGA GGAAGAAGAT TGCCCTCGAG CTGTCGGAGC TTGTTGTCTA TTGCCGGCCA 
GTTCCGTTCG ATGAGGAAAA GATTGGCACA GAGAAGGCTT GCTACCGTGA CATGTCTTCA 
TTCCCCGAGA CCAAGGCAGA GAAGTACGCC AACCGCAGCA AAGGCAAGAA ATTCCTGCAG 
TACAACCGAC GCCAGCTGAG CAGGGTTTAC CCAAAGGGCC AGCGCCTGGA CTCCTCCAAT 
TATGACCCAC TGCCCATGTG GATCTGTGGC AGCCAGCTGG TGGCTCTGAA CTTCCAGACC 
CCAGACAAGC CGATGCAGCT GAACCAAGCA CTGTTCATGC TGGGGGGCCA GAGCGGCTAT 
GTCCTGCAGC CGGACATCAT GAGAGACGAC CTTTTTGATC CCTTCGATAA GAACAGCTTG 
AAGATTGTGG AGCCAATCAC AGTGCAGCTT CAGATATTAG GAGCCAGGCA CCTCCCCAAA 
AATGGGCGGA GTATCGTTTG CCCCTTCATA GAAGTGGAAG TTTGTGGCTC AGAATTCGAC 
AACAGCAAAA GCAAAAGTGA CGTGGTGGCG GATAATGGAC TGAACCCTGT GTGGATGATG 
AAGCAGTTTG TGTTTGACAT CACCAACCCA GAGTTTGCCT TCCTGCGATT CGTGGTGTAC 
GAGGAGGACA TGTTCAGTGA CCCCAACTTC TTGGCCCAAG CCACGTTCTC TGTCAAGGGG 
CTGAAAACAG GTTACCGCTC CGTGCCGCTG AGGAACAGCT ACAGCGAAGA GTTGGAGTTG 
GCCTCACTGT TGGTGCACAT AGAGATTGTC AATGCCAAGG AGGAAGACGA CGAGAATCTC 
TACACCTCCA TCCAGCAGCT GAGGGACCGG GCCAGCGAAT TGTCCAGCCA GGTTTCCAGC 
TACGAACGGG CCAATAACTG CGACTCTCGC TACCAGCAGA GACTGGACGA GCTGCGGGCA 
GCCCAGGAGC GACTCATGGA ACTTACGGAA GTGCGCAACA GGAAGCTGAT GGAGAAGAAG 
AAGAGAGAGA GACAGTTAGC GAACAAGCGG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759899.1
CDS	457..3792
Translation	MRFLRDKMLEIEARSELTFPHFVLFYNNLMFDAQKSIIEQLELSFPLRNVDRPSLCQISLYDFQRFLQFDQKEAWAGDINKVRGYLCNYLKDSDGDVCEPILQLDEFLTYLFSKENMIMDSRFEQVVPEEMTYPLSQYWISSSHNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDDLPIIYHGHTLTSKIKFLDVLHTIKDNAFVTSEYPVILSIEDHCSVVQQRNMAHYFRKVFGDMLLTKPVDINADELPSPAQLKKKILIKHKKLVEGNLYEEVSTASYSENDISNSIKNGILYLEDPIDHTWSPHYFVLTSNKIYYSEETSRYQSNDEDEEMEPKEEFNNNELHFSEKWFHGKLGGGRDGRQIAEKLLHEYCTETGGKDGTFLVRESETFVGDYTLSFWRSSRVQHCRIHSRQEAGSTKFYLTDNLVFDSLYGLISHYREVPLRCNEFEMRLTDPVPQPNAHESKEWYHANLTRLQAEHMLMRVPRDGAFLLRKRSETSSFAISFRAEGKIKHCRIQQEGRLFMLGSSAEFESLVDLISYYEKHPLYRKMRLRYPINEEALEKMGTAELDYDALFEGRTPHVYVEANKMPTSRCTVKALYDYKAQREDELTFCKQAIIHNVDKQDGGWWRGDYGGKKQLWFPANYVEETTSAMSPDQDEGSFYFGQSVENSPLGNFLKGFIDVPSCHIVSSKDGRSSRPYVFTIHSQQMSHPSQSLDVAADTQEELNDWIGKIREATQNADARMQEGKIMERRKKIALELSELVVYCRPVPFDEEKIGTEKACYRDMSSFPETKAEKYANRSKGKKFLQYNRRQLSRVYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQLNQALFMLGGQSGYVLQPDIMRDDLFDPFDKNSLKIVEPITVQLQILGARHLPKNGRSIVCPFIEVEVCGSEFDNSKSKSDVVADNGLNPVWMMKQFVFDITNPEFAFLRFVVYEEDMFSDPNFLAQATFSVKGLKTGYRSVPLRNSYSEELELASLLVHIEIVNAKEEDDENLYTSIQQLRDRASELSSQVSSYERANNCDSRYQQRLDELRAAQERLMELTEVRNRKLMEKKKRERQLANKRN

Target ORF information:

RefSeq Version	XM_031904039.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 (LOC100496658), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031904039.1

ORF Insert Sequence:

ATGAGATTTC TACGGGACAA AATGCTGGAA ATCGAGGCCC GATCGGAGCT GACCTTCCCA 
CACTTTGTTC TGTTTTACAA TAACTTGATG TTTGATGCTC AGAAATCAAT CATTGAGCAG 
CTCGAGTTGT CTTTCCCTTT AAGGAACGTT GATCGGCCTT CTCTATGCCA GATCTCTTTG 
TATGACTTCC AGAGGTTTCT TCAGTTTGAT CAGAAGGAAG CCTGGGCCGG TGACATTAAT 
AAGGTTCGGG GTTACCTATG CAATTACCTG AAGGACAGCG ATGGGGACGT CTGTGAACCC 
ATTCTGCAGT TGGATGAGTT CCTGACCTAC CTCTTCTCCA AGGAGAACAT GATCATGGAC 
TCTAGGTTTG AGCAAGTGGT GCCGGAGGAG ATGACCTACC CGCTGTCCCA GTACTGGATC 
TCATCCTCAC ACAACACATA TTTGACTGGG GATCAGTTCT CCAGCGAGTC ATCCTTAGAA 
GCTTACGCTC GCTGCCTCCG GATGGGCTGC CGCTGCATTG AGTTGGACTG CTGGGATGGA 
CCAGATGACC TGCCTATTAT CTATCATGGA CACACCTTGA CCTCTAAAAT CAAGTTTTTG 
GATGTACTGC ACACTATCAA GGATAATGCC TTTGTCACAT CAGAATATCC GGTTATCTTG 
TCCATTGAGG ATCACTGCAG TGTTGTCCAG CAGAGGAACA TGGCCCATTA TTTCAGAAAG 
GTATTTGGGG ACATGTTACT GACCAAACCA GTGGACATCA ATGCGGACGA GCTCCCCTCT 
CCGGCCCAGC TCAAAAAGAA GATTCTCATT AAGCACAAGA AGCTGGTAGA GGGGAACCTT 
TATGAGGAGG TCTCAACCGC GAGCTACTCG GAGAATGACA TCAGTAACTC TATCAAGAAC 
GGGATCCTCT ACCTAGAAGA TCCCATAGAC CATACCTGGA GCCCTCACTA TTTCGTCTTA 
ACAAGTAACA AGATCTACTA CTCCGAGGAG ACGTCTCGGT ACCAGTCCAA CGATGAGGAC 
GAAGAGATGG AACCCAAAGA GGAGTTTAAT AACAATGAGC TGCACTTCAG TGAGAAGTGG 
TTCCACGGGA AGCTGGGGGG CGGGCGCGAC GGGCGCCAGA TTGCCGAGAA GCTGCTGCAC 
GAGTACTGCA CGGAGACGGG GGGAAAGGAC GGCACATTCC TAGTGCGTGA GAGCGAGACC 
TTCGTTGGCG ACTACACCTT GTCATTCTGG CGCTCCAGCA GGGTCCAGCA CTGCCGCATC 
CACTCTCGGC AGGAGGCCGG CTCCACCAAA TTCTACCTGA CAGACAACCT GGTGTTTGAC 
AGCCTGTATG GCCTGATCAG CCACTACCGG GAGGTGCCGC TGCGCTGCAA TGAGTTTGAG 
ATGAGGCTCA CCGACCCCGT TCCGCAGCCC AACGCACACG AGAGCAAAGA GTGGTACCAT 
GCCAATCTCA CCCGCCTGCA GGCGGAGCAT ATGCTGATGA GAGTTCCCCG AGACGGTGCC 
TTCCTGTTGC GCAAGCGGAG CGAGACCAGC TCATTCGCTA TTTCCTTCAG GGCCGAGGGC 
AAGATCAAGC ACTGCCGGAT ACAGCAGGAG GGCCGACTCT TCATGTTGGG CAGCTCCGCA 
GAGTTCGAGA GCCTGGTCGA CCTCATTAGT TACTATGAGA AGCATCCGCT GTACCGCAAG 
ATGAGGCTCC GCTATCCCAT CAACGAGGAA GCGCTGGAGA AGATGGGCAC GGCGGAACTG 
GACTACGATG CTCTGTTTGA GGGTAGAACC CCCCATGTTT ACGTGGAGGC CAACAAGATG 
CCCACATCAA GGTGCACGGT GAAAGCGCTG TACGATTACA AGGCCCAGCG GGAGGATGAG 
CTGACGTTCT GCAAGCAAGC CATTATACAC AATGTGGACA AACAAGATGG CGGCTGGTGG 
CGAGGGGATT ACGGAGGCAA AAAGCAACTT TGGTTCCCTG CTAATTACGT AGAAGAGACA 
ACGAGCGCGA TGTCCCCAGA TCAGGATGAG GGCTCTTTCT ATTTTGGCCA GTCGGTAGAG 
AACAGCCCCT TGGGGAACTT CCTGAAAGGA TTTATCGATG TGCCTTCCTG CCACATAGTC 
TCATCGAAGG ACGGAAGGAG CTCGCGGCCG TACGTCTTCA CCATCCACTC CCAGCAAATG 
TCCCACCCCT CTCAGTCCTT GGACGTGGCT GCTGACACTC AGGAAGAGCT CAATGATTGG 
ATCGGCAAAA TCCGAGAGGC CACTCAAAAC GCAGATGCTC GGATGCAGGA AGGGAAAATC 
ATGGAGCGGA GGAAGAAGAT TGCCCTCGAG CTGTCGGAGC TTGTTGTCTA TTGCCGGCCA 
GTTCCGTTCG ATGAGGAAAA GATTGGCACA GAGAAGGCTT GCTACCGTGA CATGTCTTCA 
TTCCCCGAGA CCAAGGCAGA GAAGTACGCC AACCGCAGCA AAGGCAAGAA ATTCCTGCAG 
TACAACCGAC GCCAGCTGAG CAGGGTTTAC CCAAAGGGCC AGCGCCTGGA CTCCTCCAAT 
TATGACCCAC TGCCCATGTG GATCTGTGGC AGCCAGCTGG TGGCTCTGAA CTTCCAGACC 
CCAGACAAGC CGATGCAGCT GAACCAAGCA CTGTTCATGC TGGGGGGCCA GAGCGGCTAT 
GTCCTGCAGC CGGACATCAT GAGAGACGAC CTTTTTGATC CCTTCGATAA GAACAGCTTG 
AAGATTGTGG AGCCAATCAC AGTGCAGCTT CAGATATTAG GAGCCAGGCA CCTCCCCAAA 
AATGGGCGGA GTATCGTTTG CCCCTTCATA GAAGTGGAAG TTTGTGGCTC AGAATTCGAC 
AACAGCAAAA GCAAAAGTGA CGTGGTGGCG GATAATGGAC TGAACCCTGT GTGGATGATG 
AAGCAGTTTG TGTTTGACAT CACCAACCCA GAGTTTGCCT TCCTGCGATT CGTGGTGTAC 
GAGGAGGACA TGTTCAGTGA CCCCAACTTC TTGGCCCAAG CCACGTTCTC TGTCAAGGGG 
CTGAAAACAG GTTACCGCTC CGTGCCGCTG AGGAACAGCT ACAGCGAAGA GTTGGAGTTG 
GCCTCACTGT TGGTGCACAT AGAGATTGTC AATGCCAAGG AGGAAGACGA CGAGAATCTC 
TACACCTCCA TCCAGCAGCT GAGGGACCGG GCCAGCGAAT TGTCCAGCCA GGTTTCCAGC 
TACGAACGGG CCAATAACTG CGACTCTCGC TACCAGCAGA GACTGGACGA GCTGCGGGCA 
GCCCAGGAGC GACTCATGGA ACTTACGGAA GTGCGCAACA GGAAGCTGAT GGAGAAGAAG 
AAGAGAGAGA GACAGTTAGC GAACAAGCGG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC100496658 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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