LOC100496487 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following LOC100496487 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100496487 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa17215 XM_018097824.1
Latest version!
Xenopus tropicalis otopetrin-2-like (LOC100496487), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OXa50360 XM_018097824.2
Latest version!
Xenopus tropicalis proton channel OTOP2 (LOC100496487), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa17215
    Clone ID Related Accession (Same CDS sequence) XM_018097824.1
    Accession Version XM_018097824.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product otopetrin-2-like
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCCCAACG CAGAGACAGG CCATGAAGGC CCAGAGGTAC CATATGCTCC CAGTGAAGTC 
    ATGGAGATGC CGCAGATCAG CAAGAAAGAT CTGGATGCTC CTCACACCAA CAGTGATGAC
    CAGGAAGAAG CTAACGTGCC TAATAGAGAG CAAGAGATGC CTAACAAAGC CTTGGAGATG
    CCTCCAACAG CCAAAGAAGA TATGAATGTT CCTCACATTA ACAACAAAGA TTCGGACATG
    TCTCAGCCGC CATATGAAGG TGCATTGCCC TATCTACCTG CTCAAGTTAT GAATCCGGAG
    AGTTCAGAGG CCCAGATGGA CTTAAGCCTT CAGAATCAGA CGACGGCCTC CCCATCGGGA
    CATTCCGAGG TCTGGAAGAA GGGAGGCCGT CTGCTGTCGG CACTTATGGC CATTAACATT
    GCTCTGTTTG GCAGTGTGCT TGTGAGCAGC GGGTCCCTGG AACACGTGGT TGTGCAGGAT
    AAGGAGGTGC TGGCGTTCCT TGTTGTGCTG ATGCTGCTTT CTGTTTTCTG GATGATTTTC
    CAGTTCTACT TCTCCTGCCA AAAAAACGCT GTTCTGTACA AGGATTCCCA CGCGGGGCCA
    GTGTGGCTCA GAGGGGGCCT GGTGTTCTTC GGTATCTGCA GTCTGGTGAT GGATGTGTTT
    AAGATTGGCT ATTACATCAG TTACATTGAT TGTGAGTCTC CAATCAAACT CATTCACCCC
    ATGGTACAGA GTGCGTTCAT CATCATGCAG ACGTATTTCC TATGGGTCTC CAGCAAACAC
    TGCGTGCAGA TCCACACAAA TATCTCCAGG TATGGCCTGA TGCTCATACT CATCACCAAC
    CTCAGCATCT GGATGGCGGC CGTAACAGAT GAGTCTGTCC ACCAAACCAG AGACCTCGAG
    GACGACCATA AAATTGTCCA CAGCAGCCCA GCTGGGTCCC ATGATCACAA AGAAGTCGGA
    GGAGCGCACG AACACACCTG CCAGTGCAGC AACCACCTGT GCCACATCTT CCAGACGGGG
    TACTACTACC TCTACCCATT CAACATTGAG TACAGCCTGT TCGCCTCGGC TATGACATAC
    GTCATGTGGA AGAACGTCGG CCGTGAAATG GACGACCAAG TGGCCCATCA CAGTCGCCTC
    AGTCTTTGCT TCTATTTTAA GAACATGTTT GCTGGGTTCC TAATTGGCGG GGCCGTCTTG
    CTGTGCGGCG TTGTGGTCCT TGTCATCTAC AAGGTCAAGA TCAACTTGCC GGATAAGCTG
    TACAGGGCGC ACATCCTCTT CTATTCATTT AATATATTGG CTCTGGCGCT CATGTCGTTG
    GGTTCCCTGG CTGGATCCAT TATATACCGG TTTGACAAGA GACACATGGA CGCTCATAAG
    AATCCCACTC GGACTCTGGA TGTTACCTTG CTCCTAGGAG CAGCCTTGGG TCAGTACTGC
    ATCTCTTACT ATTCCATTGT CGCTATGGTG GCCACTTCTC CTGGGGAACT GATCAATGCC
    CTAAATTTGG TCTACTCCAT ACTCATGATC GTGCAACTTA CTCTTCAGAA TACCTTCATC
    ATAGAAGGCC TTCACCGAGA ACCCTTCCAA GATGCTGCCC CACAGCCAGA GAAGGGGCTT
    ATTTTTGCCA ATGAGGCCGT GGCTCCTTCC CTGGCTCATA ATGAAACCCC AGGATTCTCA
    GCACCAGCTC CTGAATTCCC GGCCAAGCCC AAACAGAGTA TAACCGAACC CCCGTGGAAT
    CTGAAGCGCA GGATGGTTCA GGAGATCTCG CTCTTTCTGC TGCTTTGTAA CATCATTCTT
    TGGCTAATGC CAGCGTTTGG TGCCCGCCCT CAGCTGAAGA ATAACCTGGA ACGGAACTTC
    TACGGACTGT ATGTGTGGAC TATAATCACC AACATCTGCC TGCCCTTTGG CATCTTCTAC
    CGTATGCACG CGGCTGCCAG CCTCCTGGAA GTGTTCCGAA TGTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017953313.1
    CDS20..1987
    Translation

    Target ORF information:

    RefSeq Version XM_018097824.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis otopetrin-2-like (LOC100496487), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018097824.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCCCAACG CAGAGACAGG CCATGAAGGC CCAGAGGTAC CATATGCTCC CAGTGAAGTC 
    ATGGAGATGC CGCAGATCAG CAAGAAAGAT CTGGATGCTC CTCACACCAA CAGTGATGAC
    CAGGAAGAAG CTAACGTGCC TAATAGAGAG CAAGAGATGC CTAACAAAGC CTTGGAGATG
    CCTCCAACAG CCAAAGAAGA TATGAATGTT CCTCACATTA ACAACAAAGA TTCGGACATG
    TCTCAGCCGC CATATGAAGG TGCATTGCCC TATCTACCTG CTCAAGTTAT GAATCCGGAG
    AGTTCAGAGG CCCAGATGGA CTTAAGCCTT CAGAATCAGA CGACGGCCTC CCCATCGGGA
    CATTCCGAGG TCTGGAAGAA GGGAGGCCGT CTGCTGTCGG CACTTATGGC CATTAACATT
    GCTCTGTTTG GCAGTGTGCT TGTGAGCAGC GGGTCCCTGG AACACGTGGT TGTGCAGGAT
    AAGGAGGTGC TGGCGTTCCT TGTTGTGCTG ATGCTGCTTT CTGTTTTCTG GATGATTTTC
    CAGTTCTACT TCTCCTGCCA AAAAAACGCT GTTCTGTACA AGGATTCCCA CGCGGGGCCA
    GTGTGGCTCA GAGGGGGCCT GGTGTTCTTC GGTATCTGCA GTCTGGTGAT GGATGTGTTT
    AAGATTGGCT ATTACATCAG TTACATTGAT TGTGAGTCTC CAATCAAACT CATTCACCCC
    ATGGTACAGA GTGCGTTCAT CATCATGCAG ACGTATTTCC TATGGGTCTC CAGCAAACAC
    TGCGTGCAGA TCCACACAAA TATCTCCAGG TATGGCCTGA TGCTCATACT CATCACCAAC
    CTCAGCATCT GGATGGCGGC CGTAACAGAT GAGTCTGTCC ACCAAACCAG AGACCTCGAG
    GACGACCATA AAATTGTCCA CAGCAGCCCA GCTGGGTCCC ATGATCACAA AGAAGTCGGA
    GGAGCGCACG AACACACCTG CCAGTGCAGC AACCACCTGT GCCACATCTT CCAGACGGGG
    TACTACTACC TCTACCCATT CAACATTGAG TACAGCCTGT TCGCCTCGGC TATGACATAC
    GTCATGTGGA AGAACGTCGG CCGTGAAATG GACGACCAAG TGGCCCATCA CAGTCGCCTC
    AGTCTTTGCT TCTATTTTAA GAACATGTTT GCTGGGTTCC TAATTGGCGG GGCCGTCTTG
    CTGTGCGGCG TTGTGGTCCT TGTCATCTAC AAGGTCAAGA TCAACTTGCC GGATAAGCTG
    TACAGGGCGC ACATCCTCTT CTATTCATTT AATATATTGG CTCTGGCGCT CATGTCGTTG
    GGTTCCCTGG CTGGATCCAT TATATACCGG TTTGACAAGA GACACATGGA CGCTCATAAG
    AATCCCACTC GGACTCTGGA TGTTACCTTG CTCCTAGGAG CAGCCTTGGG TCAGTACTGC
    ATCTCTTACT ATTCCATTGT CGCTATGGTG GCCACTTCTC CTGGGGAACT GATCAATGCC
    CTAAATTTGG TCTACTCCAT ACTCATGATC GTGCAACTTA CTCTTCAGAA TACCTTCATC
    ATAGAAGGCC TTCACCGAGA ACCCTTCCAA GATGCTGCCC CACAGCCAGA GAAGGGGCTT
    ATTTTTGCCA ATGAGGCCGT GGCTCCTTCC CTGGCTCATA ATGAAACCCC AGGATTCTCA
    GCACCAGCTC CTGAATTCCC GGCCAAGCCC AAACAGAGTA TAACCGAACC CCCGTGGAAT
    CTGAAGCGCA GGATGGTTCA GGAGATCTCG CTCTTTCTGC TGCTTTGTAA CATCATTCTT
    TGGCTAATGC CAGCGTTTGG TGCCCGCCCT CAGCTGAAGA ATAACCTGGA ACGGAACTTC
    TACGGACTGT ATGTGTGGAC TATAATCACC AACATCTGCC TGCCCTTTGG CATCTTCTAC
    CGTATGCACG CGGCTGCCAG CCTCCTGGAA GTGTTCCGAA TGTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa50360
    Clone ID Related Accession (Same CDS sequence) XM_018097824.2
    Accession Version XM_018097824.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product proton channel OTOP2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018097824.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCCCAACG CAGAGACAGG CCATGAAGGC CCAGAGGTAC CATATGCTCC CAGTGAAGTC 
    ATGGAGATGC CGCAGATCAG CAAGAAAGAT CTGGATGCTC CTCACACCAA CAGTGATGAC
    CAGGAAGAAG CTAACATGCC CAACAGAGAA CAAGAGATGC CTAACAAAGC CTTGGAGATG
    CCTCCAACAG CCAAAGAAGG TATGAATGTT CCTCACATTA ACAACAAAGA TTCGGACATG
    TCTCAGCCGC CATATGAAGG TGCATTGCCC TATCTACCTG CTCAAGTTAT GAATCCGGAG
    AGTTCAGAGG CCCAGATGGA CTTAAGCCTT CAGAATCAGA CGACGGCCTC CCCATCGGGA
    CATTCCGAGG TCTGGAAGAA GGGAGGCCGT CTGCTGTCGG CACTTATGGC CATTAACATT
    GCTCTGTTTG GCAGTGTGCT TGTGAGCAGC GGGTCCCTGG AACACGTGGT TGTGCAGGAT
    AAGGAGGTGC TGGCGTTCCT TGTTGTGCTG ATGCTGCTTT CTGTTTTCTG GATGATTTTC
    CAGTTCTACT TCTCCTGCCA AAAAAACGCT GTTCTGTACA AGGATTCCCA CGCGGGGCCA
    GTGTGGCTCA GAGGGGGCCT GGTGTTCTTC GGTATCTGCA GTCTGGTGAT GGATGTGTTT
    AAGATTGGCT ATTACATCAG TTACATTGAT TGTGAGTCTC CAATCAAACT CATTCACCCC
    ATGGTACAGA GTGCGTTCAT CATCATGCAG ACGTATTTCC TATGGGTCTC CAGCAAACAC
    TGCGTGCAGA TCCACACAAA TATCTCCAGG TATGGCCTGA TGCTCATACT CATCACCAAC
    CTCAGCATCT GGATGGCGGC CGTAACAGAT GAGTCTGTCC ACCAAACCAG AGACCTCGAG
    GACGACCATA AAATTGTCCA CAGCAGCCCA GCTGGGTCCC ATGATCACAA AGAAGTCGGA
    GGAGCGCACG AACACACCTG CCAGTGCAGC AACCACCTGT GCCACATCTT CCAGACGGGG
    TACTACTACC TCTACCCATT CAACATTGAG TACAGCCTGT TCGCCTCGGC TATGACATAC
    GTCATGTGGA AGAACGTCGG CCGTGAAATG GACGACCAAG TGGCCCATCA CAGTCGCCTC
    AGTCTTTGCT TCTATTTTAA GAACATGTTT GCTGGGTTCC TAATTGGCGG GGCCGTCTTG
    CTGTGCGGCG TTGTGGTCCT TGTCATCTAC AAGGTCAAGA TCAACTTGCC GGATAAGCTG
    TACAGGGCGC ACATCCTCTT CTATTCATTT AATATATTGG CTCTGGCGCT CATGTCGTTG
    GGTTCCCTGG CTGGATCCAT TATATACCGG TTTGACAAGA GACACATGGA CGCTCATAAG
    AATCCCACTC GGACTCTGGA TGTTACCTTG CTCCTAGGAG CAGCCTTGGG TCAGTACTGC
    ATCTCTTACT ATTCCATTGT CGCTATGGTG GCCACTTCTC CTGGGGAACT GATCAATGCC
    CTAAATTTGG TCTACTCCAT ACTCATGATC GTGCAACTTA CTCTTCAGAA TACCTTCATC
    ATAGAAGGCC TTCACCGAGA ACCCTTCCAA GATGCTGCCC CACAGCCAGA GAAGGGGCTT
    ATTTTTGCCA ATGAGGCCGT GGCTCCTTCC CTGGCTCATA ATGAAACCCC AGGATTCTCA
    GCACCAGCTC CTGAATTCCC GGCCAAGCCC AAACAGAGTA TAACCGAACC CCCGTGGAAT
    CTGAAGCGCA GGATGGTTCA GGAGATCTCG CTCTTTCTGC TGCTTTGTAA CATCATTCTT
    TGGCTAATGC CAGCGTTTGG TGCCCGCCCT CAGCTGAAGA ATAACCTGGA ACGGAACTTC
    TACGGACTGT ATGTGTGGAC TATAATCACC AACATCTGCC TGCCCTTTGG CATCTTCTAC
    CGTATGCACG CGGCTGCCAG CCTCCTGGAA GTGTTCCGAA TGTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017953313.2
    CDS25..1992
    Translation

    Target ORF information:

    RefSeq Version XM_018097824.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis proton channel OTOP2 (LOC100496487), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018097824.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCCCAACG CAGAGACAGG CCATGAAGGC CCAGAGGTAC CATATGCTCC CAGTGAAGTC 
    ATGGAGATGC CGCAGATCAG CAAGAAAGAT CTGGATGCTC CTCACACCAA CAGTGATGAC
    CAGGAAGAAG CTAACATGCC CAACAGAGAA CAAGAGATGC CTAACAAAGC CTTGGAGATG
    CCTCCAACAG CCAAAGAAGG TATGAATGTT CCTCACATTA ACAACAAAGA TTCGGACATG
    TCTCAGCCGC CATATGAAGG TGCATTGCCC TATCTACCTG CTCAAGTTAT GAATCCGGAG
    AGTTCAGAGG CCCAGATGGA CTTAAGCCTT CAGAATCAGA CGACGGCCTC CCCATCGGGA
    CATTCCGAGG TCTGGAAGAA GGGAGGCCGT CTGCTGTCGG CACTTATGGC CATTAACATT
    GCTCTGTTTG GCAGTGTGCT TGTGAGCAGC GGGTCCCTGG AACACGTGGT TGTGCAGGAT
    AAGGAGGTGC TGGCGTTCCT TGTTGTGCTG ATGCTGCTTT CTGTTTTCTG GATGATTTTC
    CAGTTCTACT TCTCCTGCCA AAAAAACGCT GTTCTGTACA AGGATTCCCA CGCGGGGCCA
    GTGTGGCTCA GAGGGGGCCT GGTGTTCTTC GGTATCTGCA GTCTGGTGAT GGATGTGTTT
    AAGATTGGCT ATTACATCAG TTACATTGAT TGTGAGTCTC CAATCAAACT CATTCACCCC
    ATGGTACAGA GTGCGTTCAT CATCATGCAG ACGTATTTCC TATGGGTCTC CAGCAAACAC
    TGCGTGCAGA TCCACACAAA TATCTCCAGG TATGGCCTGA TGCTCATACT CATCACCAAC
    CTCAGCATCT GGATGGCGGC CGTAACAGAT GAGTCTGTCC ACCAAACCAG AGACCTCGAG
    GACGACCATA AAATTGTCCA CAGCAGCCCA GCTGGGTCCC ATGATCACAA AGAAGTCGGA
    GGAGCGCACG AACACACCTG CCAGTGCAGC AACCACCTGT GCCACATCTT CCAGACGGGG
    TACTACTACC TCTACCCATT CAACATTGAG TACAGCCTGT TCGCCTCGGC TATGACATAC
    GTCATGTGGA AGAACGTCGG CCGTGAAATG GACGACCAAG TGGCCCATCA CAGTCGCCTC
    AGTCTTTGCT TCTATTTTAA GAACATGTTT GCTGGGTTCC TAATTGGCGG GGCCGTCTTG
    CTGTGCGGCG TTGTGGTCCT TGTCATCTAC AAGGTCAAGA TCAACTTGCC GGATAAGCTG
    TACAGGGCGC ACATCCTCTT CTATTCATTT AATATATTGG CTCTGGCGCT CATGTCGTTG
    GGTTCCCTGG CTGGATCCAT TATATACCGG TTTGACAAGA GACACATGGA CGCTCATAAG
    AATCCCACTC GGACTCTGGA TGTTACCTTG CTCCTAGGAG CAGCCTTGGG TCAGTACTGC
    ATCTCTTACT ATTCCATTGT CGCTATGGTG GCCACTTCTC CTGGGGAACT GATCAATGCC
    CTAAATTTGG TCTACTCCAT ACTCATGATC GTGCAACTTA CTCTTCAGAA TACCTTCATC
    ATAGAAGGCC TTCACCGAGA ACCCTTCCAA GATGCTGCCC CACAGCCAGA GAAGGGGCTT
    ATTTTTGCCA ATGAGGCCGT GGCTCCTTCC CTGGCTCATA ATGAAACCCC AGGATTCTCA
    GCACCAGCTC CTGAATTCCC GGCCAAGCCC AAACAGAGTA TAACCGAACC CCCGTGGAAT
    CTGAAGCGCA GGATGGTTCA GGAGATCTCG CTCTTTCTGC TGCTTTGTAA CATCATTCTT
    TGGCTAATGC CAGCGTTTGG TGCCCGCCCT CAGCTGAAGA ATAACCTGGA ACGGAACTTC
    TACGGACTGT ATGTGTGGAC TATAATCACC AACATCTGCC TGCCCTTTGG CATCTTCTAC
    CGTATGCACG CGGCTGCCAG CCTCCTGGAA GTGTTCCGAA TGTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.