smpd3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	smpd3
Entrez Gene ID	100495702
Full Name	sphingomyelin phosphodiesterase 3
General protein information	Preferred Names sphingomyelin phosphodiesterase 3 Names sphingomyelin phosphodiesterase 3 sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004913485.3	XP_004913542.1	sphingomyelin phosphodiesterase 3
XM_002939876.4	XP_002939922.1	sphingomyelin phosphodiesterase 3
XM_004913486.3	XP_004913543.1	sphingomyelin phosphodiesterase 3
XM_004913485.4	XP_004913542.1	sphingomyelin phosphodiesterase 3
XM_002939876.5	XP_002939922.1	sphingomyelin phosphodiesterase 3
XM_004913486.4	XP_004913543.1	sphingomyelin phosphodiesterase 3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SMPD3	NP_001179292.1
Mus musculus (house mouse)	Smpd3	NP_067466.1
Danio rerio (zebrafish)	smpd3	NP_001116222.1
Xenopus tropicalis (tropical clawed frog)	smpd3	XP_002939922.1
Pan troglodytes (chimpanzee)	SMPD3	XP_001167790.1
Canis lupus familiaris (dog)	SMPD3	XP_546863.1
Gallus gallus (chicken)	SMPD3	XP_429344.2
Homo sapiens (human)	SMPD3	NP_061137.1
	SMPD3	XP_001091683.1
Rattus norvegicus (Norway rat)	Smpd3	NP_446057.1

Gene Ontology

The following smpd3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smpd3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa08704	XM_004913486.4 Latest version!	Xenopus tropicalis sphingomyelin phosphodiesterase 3 (smpd3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa08704	XM_004913485.4 Latest version!	Xenopus tropicalis sphingomyelin phosphodiesterase 3 (smpd3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa08704	XM_002939876.5 Latest version!	Xenopus tropicalis sphingomyelin phosphodiesterase 3 (smpd3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OXa08704	XM_004913485.3	Xenopus tropicalis sphingomyelin phosphodiesterase 3 (smpd3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa08704	XM_002939876.4	Xenopus tropicalis sphingomyelin phosphodiesterase 3 (smpd3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa08704	XM_004913486.3	Xenopus tropicalis sphingomyelin phosphodiesterase 3 (smpd3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smpd3 ( XM_004913485.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913542.1 Xenopus tropicalis sphingomyelin phosphodiesterase 3 (smpd3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa08704
Clone ID Related Accession (Same CDS sequence)	XM_004913485.3 , XM_002939876.4 , XM_004913486.3 , XM_004913485.4 , XM_002939876.5 , XM_004913486.4
Accession Version	XM_004913485.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sphingomyelin phosphodiesterase 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913485.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTTAT ACACTACCCC CTTTCCCAGC AGCTTCCTTT CGTTCCTCCA CTCCCTGTCC 
TGGGCCCTAA TTTTCCCTTG TTATTGGCTG GGAGATCGGT TACTGTCTTC ATTTTTGCCG 
ACCACCTATG AGAAACGACA ACGGTCAGAT GATCCATGTT ACTTCCAGGC CCTCTGCATT 
ATAATAACCA CACCCATTTA TTTAGCACTG CTGGTTTCAT CACTTCCGTT TGCCTTTATA 
GGATTTCTGG TGTGGTCGCC TATTCAGGCT GCACGAAGAC CCTATATCTA TTCCCATGTT 
CAGGAAAAAT CTCATGCAAC AGAAGCCAAG TTGCTGGCGG AATGGAGAGC AACAGGCAGT 
GGAAAAAGCT TCTGCTTTGG AACTGCCAAT GTTTGCCTCT TGCCTGACTC AATGGCAAGG 
TTCAATAACC TTTTTAATAC CCAAGCACGG GCTAAAGAAA TAGGGAGAAG AATACGCAAT 
GGGGCAAGTA GGCCCCAAAT TAAGATATAC ATAGACTCTC CTACTAACAC TTCTTTAAGT 
GCTGCCAGTT TTAGCAGTCT GGTGTCCCCA CAAGGTGGTG ATACATCTCA CCGAGCAGTA 
AATGTAGGTA TGAAGAGAAC AACATCTATG GAATACAAAG GGGAAAATTC TGGGGAAAAT 
AGCAGTGAGG AGGGCAGAGA TGGGAGAAAT GGAGGAGACA CAGCTGATGG AGAAGACAGT 
CCATGCATCA TTCGTATAGG TGGAGAGGAC AGTGGACACA TCTCTGACAC AGAGACTGAT 
GCTATTAGCG GGCATGTGAA TGTTGCATGC TTGGCAGAAA TTGAGCCAGA TTTTCAAAAA 
AGCCCAATTG CAAACCATAA GAATAAGGAT GCAGATGGTG GAAGTCTTGA TAGTTGTACA 
GCCTCTCGGG AGTCACTAGT CAAGGTCCGG GTGGCAGATG GAACTGCTGA CCAAAGCAGC 
ATTAACAATA AGCTTTTGTA TAAAACATCC ATAATCAAGA AGGCTACTGC CCGGAAGAAG 
AAACACACAG ATGACATTCT GGACCATGAG ATTTCTGCCT TTTTTCCTGC CAACCTGGAC 
TTTCTGTGTC TCCAGGAAGT ATTTGACAAA CGTTCTGCAA AGAAATTGAA AGAACAGTTG 
CACTACTATT TTGAATATGT GCTGTATGAT GTGGGGGTTT ATAGCTGCCA TGGGTGCTGT 
GGGTTTAAGT TCCTTAACAG TGGGCTTCTG TTTGCCAGTC GTTACCCCAT ACTAGATGCT 
ACCTATCACT GTTATCCCAA TGGGAGAGGA ATAGATGCAC TTGCTGCCAA AGGAGCTTTG 
TTTGTCAAGG TGCAAGTTGG AACCACTCCT CAGGACCAAA GAATTGTGGG ATACATCTCC 
TGCACTCACC TGCATGCTTT AGCAGGTGAT GCAATGACCC GATGTGAGCA ATTAGATATG 
CTCCAAGAAT GGGTGTCTGA ATTTCGCAAA TCTACCTCCT CCTCCAGTGC TGCAAATCCG 
GAGGAGCTGG TGGCTTTTGA CGTCATCTGT GGGGACCTTA ACTTTGACAA CTGCTCATCA 
GAAGACAAAC TGGAGCAGCA ACACTCTCTG TTTACACATT ACAAGGACCC ATGCCGGCTG 
AGCCCTGGGG AAGAAAAGCC CTGGGCAATA GGAACACTTT TGGACCCTGA GGGTCTGTAT 
GATGAGGAGG TGTGCACCCC AGATAATCTG CAGAAAGTCC TGGAGAATGA AGAAGGCAGG 
AAAGAATATT TAGTGTATCC TACCAGCAAG AACCATTGTC CCAACCAGAA GGGAAGAAAG 
GTTCCATTGA AAGGTGGTGG ACGCCGGGTG GATTATTTGT TATATACTGA GGAGGGACTC 
AACATGGAGT GGAAAGTGGA TGTAGAAGAA TACAGTTTTA TAACACAGCT AGCTGGACTT 
ACAGATCATG TGCCAGTAGC CATGCGACTG ACAGTTTCCA CAGGGGATGA AGAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913542.1
CDS	907..2886
Translation	MVLYTTPFPSSFLSFLHSLSWALIFPCYWLGDRLLSSFLPTTYEKRQRSDDPCYFQALCIIITTPIYLALLVSSLPFAFIGFLVWSPIQAARRPYIYSHVQEKSHATEAKLLAEWRATGSGKSFCFGTANVCLLPDSMARFNNLFNTQARAKEIGRRIRNGASRPQIKIYIDSPTNTSLSAASFSSLVSPQGGDTSHRAVNVGMKRTTSMEYKGENSGENSSEEGRDGRNGGDTADGEDSPCIIRIGGEDSGHISDTETDAISGHVNVACLAEIEPDFQKSPIANHKNKDADGGSLDSCTASRESLVKVRVADGTADQSSINNKLLYKTSIIKKATARKKKHTDDILDHEISAFFPANLDFLCLQEVFDKRSAKKLKEQLHYYFEYVLYDVGVYSCHGCCGFKFLNSGLLFASRYPILDATYHCYPNGRGIDALAAKGALFVKVQVGTTPQDQRIVGYISCTHLHALAGDAMTRCEQLDMLQEWVSEFRKSTSSSSAANPEELVAFDVICGDLNFDNCSSEDKLEQQHSLFTHYKDPCRLSPGEEKPWAIGTLLDPEGLYDEEVCTPDNLQKVLENEEGRKEYLVYPTSKNHCPNQKGRKVPLKGGGRRVDYLLYTEEGLNMEWKVDVEEYSFITQLAGLTDHVPVAMRLTVSTGDEES

Target ORF information:

RefSeq Version	XM_004913485.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sphingomyelin phosphodiesterase 3 (smpd3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913485.3

ORF Insert Sequence:

ATGGTTTTAT ACACTACCCC CTTTCCCAGC AGCTTCCTTT CGTTCCTCCA CTCCCTGTCC 
TGGGCCCTAA TTTTCCCTTG TTATTGGCTG GGAGATCGGT TACTGTCTTC ATTTTTGCCG 
ACCACCTATG AGAAACGACA ACGGTCAGAT GATCCATGTT ACTTCCAGGC CCTCTGCATT 
ATAATAACCA CACCCATTTA TTTAGCACTG CTGGTTTCAT CACTTCCGTT TGCCTTTATA 
GGATTTCTGG TGTGGTCGCC TATTCAGGCT GCACGAAGAC CCTATATCTA TTCCCATGTT 
CAGGAAAAAT CTCATGCAAC AGAAGCCAAG TTGCTGGCGG AATGGAGAGC AACAGGCAGT 
GGAAAAAGCT TCTGCTTTGG AACTGCCAAT GTTTGCCTCT TGCCTGACTC AATGGCAAGG 
TTCAATAACC TTTTTAATAC CCAAGCACGG GCTAAAGAAA TAGGGAGAAG AATACGCAAT 
GGGGCAAGTA GGCCCCAAAT TAAGATATAC ATAGACTCTC CTACTAACAC TTCTTTAAGT 
GCTGCCAGTT TTAGCAGTCT GGTGTCCCCA CAAGGTGGTG ATACATCTCA CCGAGCAGTA 
AATGTAGGTA TGAAGAGAAC AACATCTATG GAATACAAAG GGGAAAATTC TGGGGAAAAT 
AGCAGTGAGG AGGGCAGAGA TGGGAGAAAT GGAGGAGACA CAGCTGATGG AGAAGACAGT 
CCATGCATCA TTCGTATAGG TGGAGAGGAC AGTGGACACA TCTCTGACAC AGAGACTGAT 
GCTATTAGCG GGCATGTGAA TGTTGCATGC TTGGCAGAAA TTGAGCCAGA TTTTCAAAAA 
AGCCCAATTG CAAACCATAA GAATAAGGAT GCAGATGGTG GAAGTCTTGA TAGTTGTACA 
GCCTCTCGGG AGTCACTAGT CAAGGTCCGG GTGGCAGATG GAACTGCTGA CCAAAGCAGC 
ATTAACAATA AGCTTTTGTA TAAAACATCC ATAATCAAGA AGGCTACTGC CCGGAAGAAG 
AAACACACAG ATGACATTCT GGACCATGAG ATTTCTGCCT TTTTTCCTGC CAACCTGGAC 
TTTCTGTGTC TCCAGGAAGT ATTTGACAAA CGTTCTGCAA AGAAATTGAA AGAACAGTTG 
CACTACTATT TTGAATATGT GCTGTATGAT GTGGGGGTTT ATAGCTGCCA TGGGTGCTGT 
GGGTTTAAGT TCCTTAACAG TGGGCTTCTG TTTGCCAGTC GTTACCCCAT ACTAGATGCT 
ACCTATCACT GTTATCCCAA TGGGAGAGGA ATAGATGCAC TTGCTGCCAA AGGAGCTTTG 
TTTGTCAAGG TGCAAGTTGG AACCACTCCT CAGGACCAAA GAATTGTGGG ATACATCTCC 
TGCACTCACC TGCATGCTTT AGCAGGTGAT GCAATGACCC GATGTGAGCA ATTAGATATG 
CTCCAAGAAT GGGTGTCTGA ATTTCGCAAA TCTACCTCCT CCTCCAGTGC TGCAAATCCG 
GAGGAGCTGG TGGCTTTTGA CGTCATCTGT GGGGACCTTA ACTTTGACAA CTGCTCATCA 
GAAGACAAAC TGGAGCAGCA ACACTCTCTG TTTACACATT ACAAGGACCC ATGCCGGCTG 
AGCCCTGGGG AAGAAAAGCC CTGGGCAATA GGAACACTTT TGGACCCTGA GGGTCTGTAT 
GATGAGGAGG TGTGCACCCC AGATAATCTG CAGAAAGTCC TGGAGAATGA AGAAGGCAGG 
AAAGAATATT TAGTGTATCC TACCAGCAAG AACCATTGTC CCAACCAGAA GGGAAGAAAG 
GTTCCATTGA AAGGTGGTGG ACGCCGGGTG GATTATTTGT TATATACTGA GGAGGGACTC 
AACATGGAGT GGAAAGTGGA TGTAGAAGAA TACAGTTTTA TAACACAGCT AGCTGGACTT 
ACAGATCATG TGCCAGTAGC CATGCGACTG ACAGTTTCCA CAGGGGATGA AGAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smpd3 ( XM_002939876.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939922.1 Xenopus tropicalis sphingomyelin phosphodiesterase 3 (smpd3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa08704
Clone ID Related Accession (Same CDS sequence)	XM_004913485.3 , XM_002939876.4 , XM_004913486.3 , XM_004913485.4 , XM_002939876.5 , XM_004913486.4
Accession Version	XM_002939876.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sphingomyelin phosphodiesterase 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002939876.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTTAT ACACTACCCC CTTTCCCAGC AGCTTCCTTT CGTTCCTCCA CTCCCTGTCC 
TGGGCCCTAA TTTTCCCTTG TTATTGGCTG GGAGATCGGT TACTGTCTTC ATTTTTGCCG 
ACCACCTATG AGAAACGACA ACGGTCAGAT GATCCATGTT ACTTCCAGGC CCTCTGCATT 
ATAATAACCA CACCCATTTA TTTAGCACTG CTGGTTTCAT CACTTCCGTT TGCCTTTATA 
GGATTTCTGG TGTGGTCGCC TATTCAGGCT GCACGAAGAC CCTATATCTA TTCCCATGTT 
CAGGAAAAAT CTCATGCAAC AGAAGCCAAG TTGCTGGCGG AATGGAGAGC AACAGGCAGT 
GGAAAAAGCT TCTGCTTTGG AACTGCCAAT GTTTGCCTCT TGCCTGACTC AATGGCAAGG 
TTCAATAACC TTTTTAATAC CCAAGCACGG GCTAAAGAAA TAGGGAGAAG AATACGCAAT 
GGGGCAAGTA GGCCCCAAAT TAAGATATAC ATAGACTCTC CTACTAACAC TTCTTTAAGT 
GCTGCCAGTT TTAGCAGTCT GGTGTCCCCA CAAGGTGGTG ATACATCTCA CCGAGCAGTA 
AATGTAGGTA TGAAGAGAAC AACATCTATG GAATACAAAG GGGAAAATTC TGGGGAAAAT 
AGCAGTGAGG AGGGCAGAGA TGGGAGAAAT GGAGGAGACA CAGCTGATGG AGAAGACAGT 
CCATGCATCA TTCGTATAGG TGGAGAGGAC AGTGGACACA TCTCTGACAC AGAGACTGAT 
GCTATTAGCG GGCATGTGAA TGTTGCATGC TTGGCAGAAA TTGAGCCAGA TTTTCAAAAA 
AGCCCAATTG CAAACCATAA GAATAAGGAT GCAGATGGTG GAAGTCTTGA TAGTTGTACA 
GCCTCTCGGG AGTCACTAGT CAAGGTCCGG GTGGCAGATG GAACTGCTGA CCAAAGCAGC 
ATTAACAATA AGCTTTTGTA TAAAACATCC ATAATCAAGA AGGCTACTGC CCGGAAGAAG 
AAACACACAG ATGACATTCT GGACCATGAG ATTTCTGCCT TTTTTCCTGC CAACCTGGAC 
TTTCTGTGTC TCCAGGAAGT ATTTGACAAA CGTTCTGCAA AGAAATTGAA AGAACAGTTG 
CACTACTATT TTGAATATGT GCTGTATGAT GTGGGGGTTT ATAGCTGCCA TGGGTGCTGT 
GGGTTTAAGT TCCTTAACAG TGGGCTTCTG TTTGCCAGTC GTTACCCCAT ACTAGATGCT 
ACCTATCACT GTTATCCCAA TGGGAGAGGA ATAGATGCAC TTGCTGCCAA AGGAGCTTTG 
TTTGTCAAGG TGCAAGTTGG AACCACTCCT CAGGACCAAA GAATTGTGGG ATACATCTCC 
TGCACTCACC TGCATGCTTT AGCAGGTGAT GCAATGACCC GATGTGAGCA ATTAGATATG 
CTCCAAGAAT GGGTGTCTGA ATTTCGCAAA TCTACCTCCT CCTCCAGTGC TGCAAATCCG 
GAGGAGCTGG TGGCTTTTGA CGTCATCTGT GGGGACCTTA ACTTTGACAA CTGCTCATCA 
GAAGACAAAC TGGAGCAGCA ACACTCTCTG TTTACACATT ACAAGGACCC ATGCCGGCTG 
AGCCCTGGGG AAGAAAAGCC CTGGGCAATA GGAACACTTT TGGACCCTGA GGGTCTGTAT 
GATGAGGAGG TGTGCACCCC AGATAATCTG CAGAAAGTCC TGGAGAATGA AGAAGGCAGG 
AAAGAATATT TAGTGTATCC TACCAGCAAG AACCATTGTC CCAACCAGAA GGGAAGAAAG 
GTTCCATTGA AAGGTGGTGG ACGCCGGGTG GATTATTTGT TATATACTGA GGAGGGACTC 
AACATGGAGT GGAAAGTGGA TGTAGAAGAA TACAGTTTTA TAACACAGCT AGCTGGACTT 
ACAGATCATG TGCCAGTAGC CATGCGACTG ACAGTTTCCA CAGGGGATGA AGAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939922.1
CDS	860..2839
Translation	MVLYTTPFPSSFLSFLHSLSWALIFPCYWLGDRLLSSFLPTTYEKRQRSDDPCYFQALCIIITTPIYLALLVSSLPFAFIGFLVWSPIQAARRPYIYSHVQEKSHATEAKLLAEWRATGSGKSFCFGTANVCLLPDSMARFNNLFNTQARAKEIGRRIRNGASRPQIKIYIDSPTNTSLSAASFSSLVSPQGGDTSHRAVNVGMKRTTSMEYKGENSGENSSEEGRDGRNGGDTADGEDSPCIIRIGGEDSGHISDTETDAISGHVNVACLAEIEPDFQKSPIANHKNKDADGGSLDSCTASRESLVKVRVADGTADQSSINNKLLYKTSIIKKATARKKKHTDDILDHEISAFFPANLDFLCLQEVFDKRSAKKLKEQLHYYFEYVLYDVGVYSCHGCCGFKFLNSGLLFASRYPILDATYHCYPNGRGIDALAAKGALFVKVQVGTTPQDQRIVGYISCTHLHALAGDAMTRCEQLDMLQEWVSEFRKSTSSSSAANPEELVAFDVICGDLNFDNCSSEDKLEQQHSLFTHYKDPCRLSPGEEKPWAIGTLLDPEGLYDEEVCTPDNLQKVLENEEGRKEYLVYPTSKNHCPNQKGRKVPLKGGGRRVDYLLYTEEGLNMEWKVDVEEYSFITQLAGLTDHVPVAMRLTVSTGDEES

Target ORF information:

RefSeq Version	XM_002939876.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sphingomyelin phosphodiesterase 3 (smpd3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939876.4

ORF Insert Sequence:

ATGGTTTTAT ACACTACCCC CTTTCCCAGC AGCTTCCTTT CGTTCCTCCA CTCCCTGTCC 
TGGGCCCTAA TTTTCCCTTG TTATTGGCTG GGAGATCGGT TACTGTCTTC ATTTTTGCCG 
ACCACCTATG AGAAACGACA ACGGTCAGAT GATCCATGTT ACTTCCAGGC CCTCTGCATT 
ATAATAACCA CACCCATTTA TTTAGCACTG CTGGTTTCAT CACTTCCGTT TGCCTTTATA 
GGATTTCTGG TGTGGTCGCC TATTCAGGCT GCACGAAGAC CCTATATCTA TTCCCATGTT 
CAGGAAAAAT CTCATGCAAC AGAAGCCAAG TTGCTGGCGG AATGGAGAGC AACAGGCAGT 
GGAAAAAGCT TCTGCTTTGG AACTGCCAAT GTTTGCCTCT TGCCTGACTC AATGGCAAGG 
TTCAATAACC TTTTTAATAC CCAAGCACGG GCTAAAGAAA TAGGGAGAAG AATACGCAAT 
GGGGCAAGTA GGCCCCAAAT TAAGATATAC ATAGACTCTC CTACTAACAC TTCTTTAAGT 
GCTGCCAGTT TTAGCAGTCT GGTGTCCCCA CAAGGTGGTG ATACATCTCA CCGAGCAGTA 
AATGTAGGTA TGAAGAGAAC AACATCTATG GAATACAAAG GGGAAAATTC TGGGGAAAAT 
AGCAGTGAGG AGGGCAGAGA TGGGAGAAAT GGAGGAGACA CAGCTGATGG AGAAGACAGT 
CCATGCATCA TTCGTATAGG TGGAGAGGAC AGTGGACACA TCTCTGACAC AGAGACTGAT 
GCTATTAGCG GGCATGTGAA TGTTGCATGC TTGGCAGAAA TTGAGCCAGA TTTTCAAAAA 
AGCCCAATTG CAAACCATAA GAATAAGGAT GCAGATGGTG GAAGTCTTGA TAGTTGTACA 
GCCTCTCGGG AGTCACTAGT CAAGGTCCGG GTGGCAGATG GAACTGCTGA CCAAAGCAGC 
ATTAACAATA AGCTTTTGTA TAAAACATCC ATAATCAAGA AGGCTACTGC CCGGAAGAAG 
AAACACACAG ATGACATTCT GGACCATGAG ATTTCTGCCT TTTTTCCTGC CAACCTGGAC 
TTTCTGTGTC TCCAGGAAGT ATTTGACAAA CGTTCTGCAA AGAAATTGAA AGAACAGTTG 
CACTACTATT TTGAATATGT GCTGTATGAT GTGGGGGTTT ATAGCTGCCA TGGGTGCTGT 
GGGTTTAAGT TCCTTAACAG TGGGCTTCTG TTTGCCAGTC GTTACCCCAT ACTAGATGCT 
ACCTATCACT GTTATCCCAA TGGGAGAGGA ATAGATGCAC TTGCTGCCAA AGGAGCTTTG 
TTTGTCAAGG TGCAAGTTGG AACCACTCCT CAGGACCAAA GAATTGTGGG ATACATCTCC 
TGCACTCACC TGCATGCTTT AGCAGGTGAT GCAATGACCC GATGTGAGCA ATTAGATATG 
CTCCAAGAAT GGGTGTCTGA ATTTCGCAAA TCTACCTCCT CCTCCAGTGC TGCAAATCCG 
GAGGAGCTGG TGGCTTTTGA CGTCATCTGT GGGGACCTTA ACTTTGACAA CTGCTCATCA 
GAAGACAAAC TGGAGCAGCA ACACTCTCTG TTTACACATT ACAAGGACCC ATGCCGGCTG 
AGCCCTGGGG AAGAAAAGCC CTGGGCAATA GGAACACTTT TGGACCCTGA GGGTCTGTAT 
GATGAGGAGG TGTGCACCCC AGATAATCTG CAGAAAGTCC TGGAGAATGA AGAAGGCAGG 
AAAGAATATT TAGTGTATCC TACCAGCAAG AACCATTGTC CCAACCAGAA GGGAAGAAAG 
GTTCCATTGA AAGGTGGTGG ACGCCGGGTG GATTATTTGT TATATACTGA GGAGGGACTC 
AACATGGAGT GGAAAGTGGA TGTAGAAGAA TACAGTTTTA TAACACAGCT AGCTGGACTT 
ACAGATCATG TGCCAGTAGC CATGCGACTG ACAGTTTCCA CAGGGGATGA AGAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smpd3 ( XM_004913486.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913543.1 Xenopus tropicalis sphingomyelin phosphodiesterase 3 (smpd3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa08704
Clone ID Related Accession (Same CDS sequence)	XM_004913485.3 , XM_002939876.4 , XM_004913486.3 , XM_004913485.4 , XM_002939876.5 , XM_004913486.4
Accession Version	XM_004913486.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sphingomyelin phosphodiesterase 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913486.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTTAT ACACTACCCC CTTTCCCAGC AGCTTCCTTT CGTTCCTCCA CTCCCTGTCC 
TGGGCCCTAA TTTTCCCTTG TTATTGGCTG GGAGATCGGT TACTGTCTTC ATTTTTGCCG 
ACCACCTATG AGAAACGACA ACGGTCAGAT GATCCATGTT ACTTCCAGGC CCTCTGCATT 
ATAATAACCA CACCCATTTA TTTAGCACTG CTGGTTTCAT CACTTCCGTT TGCCTTTATA 
GGATTTCTGG TGTGGTCGCC TATTCAGGCT GCACGAAGAC CCTATATCTA TTCCCATGTT 
CAGGAAAAAT CTCATGCAAC AGAAGCCAAG TTGCTGGCGG AATGGAGAGC AACAGGCAGT 
GGAAAAAGCT TCTGCTTTGG AACTGCCAAT GTTTGCCTCT TGCCTGACTC AATGGCAAGG 
TTCAATAACC TTTTTAATAC CCAAGCACGG GCTAAAGAAA TAGGGAGAAG AATACGCAAT 
GGGGCAAGTA GGCCCCAAAT TAAGATATAC ATAGACTCTC CTACTAACAC TTCTTTAAGT 
GCTGCCAGTT TTAGCAGTCT GGTGTCCCCA CAAGGTGGTG ATACATCTCA CCGAGCAGTA 
AATGTAGGTA TGAAGAGAAC AACATCTATG GAATACAAAG GGGAAAATTC TGGGGAAAAT 
AGCAGTGAGG AGGGCAGAGA TGGGAGAAAT GGAGGAGACA CAGCTGATGG AGAAGACAGT 
CCATGCATCA TTCGTATAGG TGGAGAGGAC AGTGGACACA TCTCTGACAC AGAGACTGAT 
GCTATTAGCG GGCATGTGAA TGTTGCATGC TTGGCAGAAA TTGAGCCAGA TTTTCAAAAA 
AGCCCAATTG CAAACCATAA GAATAAGGAT GCAGATGGTG GAAGTCTTGA TAGTTGTACA 
GCCTCTCGGG AGTCACTAGT CAAGGTCCGG GTGGCAGATG GAACTGCTGA CCAAAGCAGC 
ATTAACAATA AGCTTTTGTA TAAAACATCC ATAATCAAGA AGGCTACTGC CCGGAAGAAG 
AAACACACAG ATGACATTCT GGACCATGAG ATTTCTGCCT TTTTTCCTGC CAACCTGGAC 
TTTCTGTGTC TCCAGGAAGT ATTTGACAAA CGTTCTGCAA AGAAATTGAA AGAACAGTTG 
CACTACTATT TTGAATATGT GCTGTATGAT GTGGGGGTTT ATAGCTGCCA TGGGTGCTGT 
GGGTTTAAGT TCCTTAACAG TGGGCTTCTG TTTGCCAGTC GTTACCCCAT ACTAGATGCT 
ACCTATCACT GTTATCCCAA TGGGAGAGGA ATAGATGCAC TTGCTGCCAA AGGAGCTTTG 
TTTGTCAAGG TGCAAGTTGG AACCACTCCT CAGGACCAAA GAATTGTGGG ATACATCTCC 
TGCACTCACC TGCATGCTTT AGCAGGTGAT GCAATGACCC GATGTGAGCA ATTAGATATG 
CTCCAAGAAT GGGTGTCTGA ATTTCGCAAA TCTACCTCCT CCTCCAGTGC TGCAAATCCG 
GAGGAGCTGG TGGCTTTTGA CGTCATCTGT GGGGACCTTA ACTTTGACAA CTGCTCATCA 
GAAGACAAAC TGGAGCAGCA ACACTCTCTG TTTACACATT ACAAGGACCC ATGCCGGCTG 
AGCCCTGGGG AAGAAAAGCC CTGGGCAATA GGAACACTTT TGGACCCTGA GGGTCTGTAT 
GATGAGGAGG TGTGCACCCC AGATAATCTG CAGAAAGTCC TGGAGAATGA AGAAGGCAGG 
AAAGAATATT TAGTGTATCC TACCAGCAAG AACCATTGTC CCAACCAGAA GGGAAGAAAG 
GTTCCATTGA AAGGTGGTGG ACGCCGGGTG GATTATTTGT TATATACTGA GGAGGGACTC 
AACATGGAGT GGAAAGTGGA TGTAGAAGAA TACAGTTTTA TAACACAGCT AGCTGGACTT 
ACAGATCATG TGCCAGTAGC CATGCGACTG ACAGTTTCCA CAGGGGATGA AGAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913543.1
CDS	911..2890
Translation	MVLYTTPFPSSFLSFLHSLSWALIFPCYWLGDRLLSSFLPTTYEKRQRSDDPCYFQALCIIITTPIYLALLVSSLPFAFIGFLVWSPIQAARRPYIYSHVQEKSHATEAKLLAEWRATGSGKSFCFGTANVCLLPDSMARFNNLFNTQARAKEIGRRIRNGASRPQIKIYIDSPTNTSLSAASFSSLVSPQGGDTSHRAVNVGMKRTTSMEYKGENSGENSSEEGRDGRNGGDTADGEDSPCIIRIGGEDSGHISDTETDAISGHVNVACLAEIEPDFQKSPIANHKNKDADGGSLDSCTASRESLVKVRVADGTADQSSINNKLLYKTSIIKKATARKKKHTDDILDHEISAFFPANLDFLCLQEVFDKRSAKKLKEQLHYYFEYVLYDVGVYSCHGCCGFKFLNSGLLFASRYPILDATYHCYPNGRGIDALAAKGALFVKVQVGTTPQDQRIVGYISCTHLHALAGDAMTRCEQLDMLQEWVSEFRKSTSSSSAANPEELVAFDVICGDLNFDNCSSEDKLEQQHSLFTHYKDPCRLSPGEEKPWAIGTLLDPEGLYDEEVCTPDNLQKVLENEEGRKEYLVYPTSKNHCPNQKGRKVPLKGGGRRVDYLLYTEEGLNMEWKVDVEEYSFITQLAGLTDHVPVAMRLTVSTGDEES

Target ORF information:

RefSeq Version	XM_004913486.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sphingomyelin phosphodiesterase 3 (smpd3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913486.3

ORF Insert Sequence:

ATGGTTTTAT ACACTACCCC CTTTCCCAGC AGCTTCCTTT CGTTCCTCCA CTCCCTGTCC 
TGGGCCCTAA TTTTCCCTTG TTATTGGCTG GGAGATCGGT TACTGTCTTC ATTTTTGCCG 
ACCACCTATG AGAAACGACA ACGGTCAGAT GATCCATGTT ACTTCCAGGC CCTCTGCATT 
ATAATAACCA CACCCATTTA TTTAGCACTG CTGGTTTCAT CACTTCCGTT TGCCTTTATA 
GGATTTCTGG TGTGGTCGCC TATTCAGGCT GCACGAAGAC CCTATATCTA TTCCCATGTT 
CAGGAAAAAT CTCATGCAAC AGAAGCCAAG TTGCTGGCGG AATGGAGAGC AACAGGCAGT 
GGAAAAAGCT TCTGCTTTGG AACTGCCAAT GTTTGCCTCT TGCCTGACTC AATGGCAAGG 
TTCAATAACC TTTTTAATAC CCAAGCACGG GCTAAAGAAA TAGGGAGAAG AATACGCAAT 
GGGGCAAGTA GGCCCCAAAT TAAGATATAC ATAGACTCTC CTACTAACAC TTCTTTAAGT 
GCTGCCAGTT TTAGCAGTCT GGTGTCCCCA CAAGGTGGTG ATACATCTCA CCGAGCAGTA 
AATGTAGGTA TGAAGAGAAC AACATCTATG GAATACAAAG GGGAAAATTC TGGGGAAAAT 
AGCAGTGAGG AGGGCAGAGA TGGGAGAAAT GGAGGAGACA CAGCTGATGG AGAAGACAGT 
CCATGCATCA TTCGTATAGG TGGAGAGGAC AGTGGACACA TCTCTGACAC AGAGACTGAT 
GCTATTAGCG GGCATGTGAA TGTTGCATGC TTGGCAGAAA TTGAGCCAGA TTTTCAAAAA 
AGCCCAATTG CAAACCATAA GAATAAGGAT GCAGATGGTG GAAGTCTTGA TAGTTGTACA 
GCCTCTCGGG AGTCACTAGT CAAGGTCCGG GTGGCAGATG GAACTGCTGA CCAAAGCAGC 
ATTAACAATA AGCTTTTGTA TAAAACATCC ATAATCAAGA AGGCTACTGC CCGGAAGAAG 
AAACACACAG ATGACATTCT GGACCATGAG ATTTCTGCCT TTTTTCCTGC CAACCTGGAC 
TTTCTGTGTC TCCAGGAAGT ATTTGACAAA CGTTCTGCAA AGAAATTGAA AGAACAGTTG 
CACTACTATT TTGAATATGT GCTGTATGAT GTGGGGGTTT ATAGCTGCCA TGGGTGCTGT 
GGGTTTAAGT TCCTTAACAG TGGGCTTCTG TTTGCCAGTC GTTACCCCAT ACTAGATGCT 
ACCTATCACT GTTATCCCAA TGGGAGAGGA ATAGATGCAC TTGCTGCCAA AGGAGCTTTG 
TTTGTCAAGG TGCAAGTTGG AACCACTCCT CAGGACCAAA GAATTGTGGG ATACATCTCC 
TGCACTCACC TGCATGCTTT AGCAGGTGAT GCAATGACCC GATGTGAGCA ATTAGATATG 
CTCCAAGAAT GGGTGTCTGA ATTTCGCAAA TCTACCTCCT CCTCCAGTGC TGCAAATCCG 
GAGGAGCTGG TGGCTTTTGA CGTCATCTGT GGGGACCTTA ACTTTGACAA CTGCTCATCA 
GAAGACAAAC TGGAGCAGCA ACACTCTCTG TTTACACATT ACAAGGACCC ATGCCGGCTG 
AGCCCTGGGG AAGAAAAGCC CTGGGCAATA GGAACACTTT TGGACCCTGA GGGTCTGTAT 
GATGAGGAGG TGTGCACCCC AGATAATCTG CAGAAAGTCC TGGAGAATGA AGAAGGCAGG 
AAAGAATATT TAGTGTATCC TACCAGCAAG AACCATTGTC CCAACCAGAA GGGAAGAAAG 
GTTCCATTGA AAGGTGGTGG ACGCCGGGTG GATTATTTGT TATATACTGA GGAGGGACTC 
AACATGGAGT GGAAAGTGGA TGTAGAAGAA TACAGTTTTA TAACACAGCT AGCTGGACTT 
ACAGATCATG TGCCAGTAGC CATGCGACTG ACAGTTTCCA CAGGGGATGA AGAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smpd3 ( XM_004913485.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913542.1 Xenopus tropicalis sphingomyelin phosphodiesterase 3 (smpd3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa08704
Clone ID Related Accession (Same CDS sequence)	XM_004913485.3 , XM_002939876.4 , XM_004913486.3 , XM_004913485.4 , XM_002939876.5 , XM_004913486.4
Accession Version	XM_004913485.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sphingomyelin phosphodiesterase 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913485.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTTAT ACACTACCCC CTTTCCCAGC AGCTTCCTTT CGTTCCTCCA CTCCCTGTCC 
TGGGCCCTAA TTTTCCCTTG TTATTGGCTG GGAGATCGGT TACTGTCTTC ATTTTTGCCG 
ACCACCTATG AGAAACGACA ACGGTCAGAT GATCCATGTT ACTTCCAGGC CCTCTGCATT 
ATAATAACCA CACCCATTTA TTTAGCACTG CTGGTTTCAT CACTTCCGTT TGCCTTTATA 
GGATTTCTGG TGTGGTCGCC TATTCAGGCT GCACGAAGAC CCTATATCTA TTCCCATGTT 
CAGGAAAAAT CTCATGCAAC AGAAGCCAAG TTGCTGGCGG AATGGAGAGC AACAGGCAGT 
GGAAAAAGCT TCTGCTTTGG AACTGCCAAT GTTTGCCTCT TGCCTGACTC AATGGCAAGG 
TTCAATAACC TTTTTAATAC CCAAGCACGG GCTAAAGAAA TAGGGAGAAG AATACGCAAT 
GGGGCAAGTA GGCCCCAAAT TAAGATATAC ATAGACTCTC CTACTAACAC TTCTTTAAGT 
GCTGCCAGTT TTAGCAGTCT GGTGTCCCCA CAAGGTGGTG ATACATCTCA CCGAGCAGTA 
AATGTAGGTA TGAAGAGAAC AACATCTATG GAATACAAAG GGGAAAATTC TGGGGAAAAT 
AGCAGTGAGG AGGGCAGAGA TGGGAGAAAT GGAGGAGACA CAGCTGATGG AGAAGACAGT 
CCATGCATCA TTCGTATAGG TGGAGAGGAC AGTGGACACA TCTCTGACAC AGAGACTGAT 
GCTATTAGCG GGCATGTGAA TGTTGCATGC TTGGCAGAAA TTGAGCCAGA TTTTCAAAAA 
AGCCCAATTG CAAACCATAA GAATAAGGAT GCAGATGGTG GAAGTCTTGA TAGTTGTACA 
GCCTCTCGGG AGTCACTAGT CAAGGTCCGG GTGGCAGATG GAACTGCTGA CCAAAGCAGC 
ATTAACAATA AGCTTTTGTA TAAAACATCC ATAATCAAGA AGGCTACTGC CCGGAAGAAG 
AAACACACAG ATGACATTCT GGACCATGAG ATTTCTGCCT TTTTTCCTGC CAACCTGGAC 
TTTCTGTGTC TCCAGGAAGT ATTTGACAAA CGTTCTGCAA AGAAATTGAA AGAACAGTTG 
CACTACTATT TTGAATATGT GCTGTATGAT GTGGGGGTTT ATAGCTGCCA TGGGTGCTGT 
GGGTTTAAGT TCCTTAACAG TGGGCTTCTG TTTGCCAGTC GTTACCCCAT ACTAGATGCT 
ACCTATCACT GTTATCCCAA TGGGAGAGGA ATAGATGCAC TTGCTGCCAA AGGAGCTTTG 
TTTGTCAAGG TGCAAGTTGG AACCACTCCT CAGGACCAAA GAATTGTGGG ATACATCTCC 
TGCACTCACC TGCATGCTTT AGCAGGTGAT GCAATGACCC GATGTGAGCA ATTAGATATG 
CTCCAAGAAT GGGTGTCTGA ATTTCGCAAA TCTACCTCCT CCTCCAGTGC TGCAAATCCG 
GAGGAGCTGG TGGCTTTTGA CGTCATCTGT GGGGACCTTA ACTTTGACAA CTGCTCATCA 
GAAGACAAAC TGGAGCAGCA ACACTCTCTG TTTACACATT ACAAGGACCC ATGCCGGCTG 
AGCCCTGGGG AAGAAAAGCC CTGGGCAATA GGAACACTTT TGGACCCTGA GGGTCTGTAT 
GATGAGGAGG TGTGCACCCC AGATAATCTG CAGAAAGTCC TGGAGAATGA AGAAGGCAGG 
AAAGAATATT TAGTGTATCC TACCAGCAAG AACCATTGTC CCAACCAGAA GGGAAGAAAG 
GTTCCATTGA AAGGTGGTGG ACGCCGGGTG GATTATTTGT TATATACTGA GGAGGGACTC 
AACATGGAGT GGAAAGTGGA TGTAGAAGAA TACAGTTTTA TAACACAGCT AGCTGGACTT 
ACAGATCATG TGCCAGTAGC CATGCGACTG ACAGTTTCCA CAGGGGATGA AGAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913542.1
CDS	827..2806
Translation	MVLYTTPFPSSFLSFLHSLSWALIFPCYWLGDRLLSSFLPTTYEKRQRSDDPCYFQALCIIITTPIYLALLVSSLPFAFIGFLVWSPIQAARRPYIYSHVQEKSHATEAKLLAEWRATGSGKSFCFGTANVCLLPDSMARFNNLFNTQARAKEIGRRIRNGASRPQIKIYIDSPTNTSLSAASFSSLVSPQGGDTSHRAVNVGMKRTTSMEYKGENSGENSSEEGRDGRNGGDTADGEDSPCIIRIGGEDSGHISDTETDAISGHVNVACLAEIEPDFQKSPIANHKNKDADGGSLDSCTASRESLVKVRVADGTADQSSINNKLLYKTSIIKKATARKKKHTDDILDHEISAFFPANLDFLCLQEVFDKRSAKKLKEQLHYYFEYVLYDVGVYSCHGCCGFKFLNSGLLFASRYPILDATYHCYPNGRGIDALAAKGALFVKVQVGTTPQDQRIVGYISCTHLHALAGDAMTRCEQLDMLQEWVSEFRKSTSSSSAANPEELVAFDVICGDLNFDNCSSEDKLEQQHSLFTHYKDPCRLSPGEEKPWAIGTLLDPEGLYDEEVCTPDNLQKVLENEEGRKEYLVYPTSKNHCPNQKGRKVPLKGGGRRVDYLLYTEEGLNMEWKVDVEEYSFITQLAGLTDHVPVAMRLTVSTGDEES

Target ORF information:

RefSeq Version	XM_004913485.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sphingomyelin phosphodiesterase 3 (smpd3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913485.4

ORF Insert Sequence:

ATGGTTTTAT ACACTACCCC CTTTCCCAGC AGCTTCCTTT CGTTCCTCCA CTCCCTGTCC 
TGGGCCCTAA TTTTCCCTTG TTATTGGCTG GGAGATCGGT TACTGTCTTC ATTTTTGCCG 
ACCACCTATG AGAAACGACA ACGGTCAGAT GATCCATGTT ACTTCCAGGC CCTCTGCATT 
ATAATAACCA CACCCATTTA TTTAGCACTG CTGGTTTCAT CACTTCCGTT TGCCTTTATA 
GGATTTCTGG TGTGGTCGCC TATTCAGGCT GCACGAAGAC CCTATATCTA TTCCCATGTT 
CAGGAAAAAT CTCATGCAAC AGAAGCCAAG TTGCTGGCGG AATGGAGAGC AACAGGCAGT 
GGAAAAAGCT TCTGCTTTGG AACTGCCAAT GTTTGCCTCT TGCCTGACTC AATGGCAAGG 
TTCAATAACC TTTTTAATAC CCAAGCACGG GCTAAAGAAA TAGGGAGAAG AATACGCAAT 
GGGGCAAGTA GGCCCCAAAT TAAGATATAC ATAGACTCTC CTACTAACAC TTCTTTAAGT 
GCTGCCAGTT TTAGCAGTCT GGTGTCCCCA CAAGGTGGTG ATACATCTCA CCGAGCAGTA 
AATGTAGGTA TGAAGAGAAC AACATCTATG GAATACAAAG GGGAAAATTC TGGGGAAAAT 
AGCAGTGAGG AGGGCAGAGA TGGGAGAAAT GGAGGAGACA CAGCTGATGG AGAAGACAGT 
CCATGCATCA TTCGTATAGG TGGAGAGGAC AGTGGACACA TCTCTGACAC AGAGACTGAT 
GCTATTAGCG GGCATGTGAA TGTTGCATGC TTGGCAGAAA TTGAGCCAGA TTTTCAAAAA 
AGCCCAATTG CAAACCATAA GAATAAGGAT GCAGATGGTG GAAGTCTTGA TAGTTGTACA 
GCCTCTCGGG AGTCACTAGT CAAGGTCCGG GTGGCAGATG GAACTGCTGA CCAAAGCAGC 
ATTAACAATA AGCTTTTGTA TAAAACATCC ATAATCAAGA AGGCTACTGC CCGGAAGAAG 
AAACACACAG ATGACATTCT GGACCATGAG ATTTCTGCCT TTTTTCCTGC CAACCTGGAC 
TTTCTGTGTC TCCAGGAAGT ATTTGACAAA CGTTCTGCAA AGAAATTGAA AGAACAGTTG 
CACTACTATT TTGAATATGT GCTGTATGAT GTGGGGGTTT ATAGCTGCCA TGGGTGCTGT 
GGGTTTAAGT TCCTTAACAG TGGGCTTCTG TTTGCCAGTC GTTACCCCAT ACTAGATGCT 
ACCTATCACT GTTATCCCAA TGGGAGAGGA ATAGATGCAC TTGCTGCCAA AGGAGCTTTG 
TTTGTCAAGG TGCAAGTTGG AACCACTCCT CAGGACCAAA GAATTGTGGG ATACATCTCC 
TGCACTCACC TGCATGCTTT AGCAGGTGAT GCAATGACCC GATGTGAGCA ATTAGATATG 
CTCCAAGAAT GGGTGTCTGA ATTTCGCAAA TCTACCTCCT CCTCCAGTGC TGCAAATCCG 
GAGGAGCTGG TGGCTTTTGA CGTCATCTGT GGGGACCTTA ACTTTGACAA CTGCTCATCA 
GAAGACAAAC TGGAGCAGCA ACACTCTCTG TTTACACATT ACAAGGACCC ATGCCGGCTG 
AGCCCTGGGG AAGAAAAGCC CTGGGCAATA GGAACACTTT TGGACCCTGA GGGTCTGTAT 
GATGAGGAGG TGTGCACCCC AGATAATCTG CAGAAAGTCC TGGAGAATGA AGAAGGCAGG 
AAAGAATATT TAGTGTATCC TACCAGCAAG AACCATTGTC CCAACCAGAA GGGAAGAAAG 
GTTCCATTGA AAGGTGGTGG ACGCCGGGTG GATTATTTGT TATATACTGA GGAGGGACTC 
AACATGGAGT GGAAAGTGGA TGTAGAAGAA TACAGTTTTA TAACACAGCT AGCTGGACTT 
ACAGATCATG TGCCAGTAGC CATGCGACTG ACAGTTTCCA CAGGGGATGA AGAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smpd3 ( XM_002939876.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939922.1 Xenopus tropicalis sphingomyelin phosphodiesterase 3 (smpd3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa08704
Clone ID Related Accession (Same CDS sequence)	XM_004913485.3 , XM_002939876.4 , XM_004913486.3 , XM_004913485.4 , XM_002939876.5 , XM_004913486.4
Accession Version	XM_002939876.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sphingomyelin phosphodiesterase 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002939876.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTTAT ACACTACCCC CTTTCCCAGC AGCTTCCTTT CGTTCCTCCA CTCCCTGTCC 
TGGGCCCTAA TTTTCCCTTG TTATTGGCTG GGAGATCGGT TACTGTCTTC ATTTTTGCCG 
ACCACCTATG AGAAACGACA ACGGTCAGAT GATCCATGTT ACTTCCAGGC CCTCTGCATT 
ATAATAACCA CACCCATTTA TTTAGCACTG CTGGTTTCAT CACTTCCGTT TGCCTTTATA 
GGATTTCTGG TGTGGTCGCC TATTCAGGCT GCACGAAGAC CCTATATCTA TTCCCATGTT 
CAGGAAAAAT CTCATGCAAC AGAAGCCAAG TTGCTGGCGG AATGGAGAGC AACAGGCAGT 
GGAAAAAGCT TCTGCTTTGG AACTGCCAAT GTTTGCCTCT TGCCTGACTC AATGGCAAGG 
TTCAATAACC TTTTTAATAC CCAAGCACGG GCTAAAGAAA TAGGGAGAAG AATACGCAAT 
GGGGCAAGTA GGCCCCAAAT TAAGATATAC ATAGACTCTC CTACTAACAC TTCTTTAAGT 
GCTGCCAGTT TTAGCAGTCT GGTGTCCCCA CAAGGTGGTG ATACATCTCA CCGAGCAGTA 
AATGTAGGTA TGAAGAGAAC AACATCTATG GAATACAAAG GGGAAAATTC TGGGGAAAAT 
AGCAGTGAGG AGGGCAGAGA TGGGAGAAAT GGAGGAGACA CAGCTGATGG AGAAGACAGT 
CCATGCATCA TTCGTATAGG TGGAGAGGAC AGTGGACACA TCTCTGACAC AGAGACTGAT 
GCTATTAGCG GGCATGTGAA TGTTGCATGC TTGGCAGAAA TTGAGCCAGA TTTTCAAAAA 
AGCCCAATTG CAAACCATAA GAATAAGGAT GCAGATGGTG GAAGTCTTGA TAGTTGTACA 
GCCTCTCGGG AGTCACTAGT CAAGGTCCGG GTGGCAGATG GAACTGCTGA CCAAAGCAGC 
ATTAACAATA AGCTTTTGTA TAAAACATCC ATAATCAAGA AGGCTACTGC CCGGAAGAAG 
AAACACACAG ATGACATTCT GGACCATGAG ATTTCTGCCT TTTTTCCTGC CAACCTGGAC 
TTTCTGTGTC TCCAGGAAGT ATTTGACAAA CGTTCTGCAA AGAAATTGAA AGAACAGTTG 
CACTACTATT TTGAATATGT GCTGTATGAT GTGGGGGTTT ATAGCTGCCA TGGGTGCTGT 
GGGTTTAAGT TCCTTAACAG TGGGCTTCTG TTTGCCAGTC GTTACCCCAT ACTAGATGCT 
ACCTATCACT GTTATCCCAA TGGGAGAGGA ATAGATGCAC TTGCTGCCAA AGGAGCTTTG 
TTTGTCAAGG TGCAAGTTGG AACCACTCCT CAGGACCAAA GAATTGTGGG ATACATCTCC 
TGCACTCACC TGCATGCTTT AGCAGGTGAT GCAATGACCC GATGTGAGCA ATTAGATATG 
CTCCAAGAAT GGGTGTCTGA ATTTCGCAAA TCTACCTCCT CCTCCAGTGC TGCAAATCCG 
GAGGAGCTGG TGGCTTTTGA CGTCATCTGT GGGGACCTTA ACTTTGACAA CTGCTCATCA 
GAAGACAAAC TGGAGCAGCA ACACTCTCTG TTTACACATT ACAAGGACCC ATGCCGGCTG 
AGCCCTGGGG AAGAAAAGCC CTGGGCAATA GGAACACTTT TGGACCCTGA GGGTCTGTAT 
GATGAGGAGG TGTGCACCCC AGATAATCTG CAGAAAGTCC TGGAGAATGA AGAAGGCAGG 
AAAGAATATT TAGTGTATCC TACCAGCAAG AACCATTGTC CCAACCAGAA GGGAAGAAAG 
GTTCCATTGA AAGGTGGTGG ACGCCGGGTG GATTATTTGT TATATACTGA GGAGGGACTC 
AACATGGAGT GGAAAGTGGA TGTAGAAGAA TACAGTTTTA TAACACAGCT AGCTGGACTT 
ACAGATCATG TGCCAGTAGC CATGCGACTG ACAGTTTCCA CAGGGGATGA AGAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939922.1
CDS	780..2759
Translation	MVLYTTPFPSSFLSFLHSLSWALIFPCYWLGDRLLSSFLPTTYEKRQRSDDPCYFQALCIIITTPIYLALLVSSLPFAFIGFLVWSPIQAARRPYIYSHVQEKSHATEAKLLAEWRATGSGKSFCFGTANVCLLPDSMARFNNLFNTQARAKEIGRRIRNGASRPQIKIYIDSPTNTSLSAASFSSLVSPQGGDTSHRAVNVGMKRTTSMEYKGENSGENSSEEGRDGRNGGDTADGEDSPCIIRIGGEDSGHISDTETDAISGHVNVACLAEIEPDFQKSPIANHKNKDADGGSLDSCTASRESLVKVRVADGTADQSSINNKLLYKTSIIKKATARKKKHTDDILDHEISAFFPANLDFLCLQEVFDKRSAKKLKEQLHYYFEYVLYDVGVYSCHGCCGFKFLNSGLLFASRYPILDATYHCYPNGRGIDALAAKGALFVKVQVGTTPQDQRIVGYISCTHLHALAGDAMTRCEQLDMLQEWVSEFRKSTSSSSAANPEELVAFDVICGDLNFDNCSSEDKLEQQHSLFTHYKDPCRLSPGEEKPWAIGTLLDPEGLYDEEVCTPDNLQKVLENEEGRKEYLVYPTSKNHCPNQKGRKVPLKGGGRRVDYLLYTEEGLNMEWKVDVEEYSFITQLAGLTDHVPVAMRLTVSTGDEES

Target ORF information:

RefSeq Version	XM_002939876.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sphingomyelin phosphodiesterase 3 (smpd3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939876.5

ORF Insert Sequence:

ATGGTTTTAT ACACTACCCC CTTTCCCAGC AGCTTCCTTT CGTTCCTCCA CTCCCTGTCC 
TGGGCCCTAA TTTTCCCTTG TTATTGGCTG GGAGATCGGT TACTGTCTTC ATTTTTGCCG 
ACCACCTATG AGAAACGACA ACGGTCAGAT GATCCATGTT ACTTCCAGGC CCTCTGCATT 
ATAATAACCA CACCCATTTA TTTAGCACTG CTGGTTTCAT CACTTCCGTT TGCCTTTATA 
GGATTTCTGG TGTGGTCGCC TATTCAGGCT GCACGAAGAC CCTATATCTA TTCCCATGTT 
CAGGAAAAAT CTCATGCAAC AGAAGCCAAG TTGCTGGCGG AATGGAGAGC AACAGGCAGT 
GGAAAAAGCT TCTGCTTTGG AACTGCCAAT GTTTGCCTCT TGCCTGACTC AATGGCAAGG 
TTCAATAACC TTTTTAATAC CCAAGCACGG GCTAAAGAAA TAGGGAGAAG AATACGCAAT 
GGGGCAAGTA GGCCCCAAAT TAAGATATAC ATAGACTCTC CTACTAACAC TTCTTTAAGT 
GCTGCCAGTT TTAGCAGTCT GGTGTCCCCA CAAGGTGGTG ATACATCTCA CCGAGCAGTA 
AATGTAGGTA TGAAGAGAAC AACATCTATG GAATACAAAG GGGAAAATTC TGGGGAAAAT 
AGCAGTGAGG AGGGCAGAGA TGGGAGAAAT GGAGGAGACA CAGCTGATGG AGAAGACAGT 
CCATGCATCA TTCGTATAGG TGGAGAGGAC AGTGGACACA TCTCTGACAC AGAGACTGAT 
GCTATTAGCG GGCATGTGAA TGTTGCATGC TTGGCAGAAA TTGAGCCAGA TTTTCAAAAA 
AGCCCAATTG CAAACCATAA GAATAAGGAT GCAGATGGTG GAAGTCTTGA TAGTTGTACA 
GCCTCTCGGG AGTCACTAGT CAAGGTCCGG GTGGCAGATG GAACTGCTGA CCAAAGCAGC 
ATTAACAATA AGCTTTTGTA TAAAACATCC ATAATCAAGA AGGCTACTGC CCGGAAGAAG 
AAACACACAG ATGACATTCT GGACCATGAG ATTTCTGCCT TTTTTCCTGC CAACCTGGAC 
TTTCTGTGTC TCCAGGAAGT ATTTGACAAA CGTTCTGCAA AGAAATTGAA AGAACAGTTG 
CACTACTATT TTGAATATGT GCTGTATGAT GTGGGGGTTT ATAGCTGCCA TGGGTGCTGT 
GGGTTTAAGT TCCTTAACAG TGGGCTTCTG TTTGCCAGTC GTTACCCCAT ACTAGATGCT 
ACCTATCACT GTTATCCCAA TGGGAGAGGA ATAGATGCAC TTGCTGCCAA AGGAGCTTTG 
TTTGTCAAGG TGCAAGTTGG AACCACTCCT CAGGACCAAA GAATTGTGGG ATACATCTCC 
TGCACTCACC TGCATGCTTT AGCAGGTGAT GCAATGACCC GATGTGAGCA ATTAGATATG 
CTCCAAGAAT GGGTGTCTGA ATTTCGCAAA TCTACCTCCT CCTCCAGTGC TGCAAATCCG 
GAGGAGCTGG TGGCTTTTGA CGTCATCTGT GGGGACCTTA ACTTTGACAA CTGCTCATCA 
GAAGACAAAC TGGAGCAGCA ACACTCTCTG TTTACACATT ACAAGGACCC ATGCCGGCTG 
AGCCCTGGGG AAGAAAAGCC CTGGGCAATA GGAACACTTT TGGACCCTGA GGGTCTGTAT 
GATGAGGAGG TGTGCACCCC AGATAATCTG CAGAAAGTCC TGGAGAATGA AGAAGGCAGG 
AAAGAATATT TAGTGTATCC TACCAGCAAG AACCATTGTC CCAACCAGAA GGGAAGAAAG 
GTTCCATTGA AAGGTGGTGG ACGCCGGGTG GATTATTTGT TATATACTGA GGAGGGACTC 
AACATGGAGT GGAAAGTGGA TGTAGAAGAA TACAGTTTTA TAACACAGCT AGCTGGACTT 
ACAGATCATG TGCCAGTAGC CATGCGACTG ACAGTTTCCA CAGGGGATGA AGAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smpd3 ( XM_004913486.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913543.1 Xenopus tropicalis sphingomyelin phosphodiesterase 3 (smpd3), transcript variant X2, mRNA.

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CloneID	OXa08704
Clone ID Related Accession (Same CDS sequence)	XM_004913485.3 , XM_002939876.4 , XM_004913486.3 , XM_004913485.4 , XM_002939876.5 , XM_004913486.4
Accession Version	XM_004913486.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sphingomyelin phosphodiesterase 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913486.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTTAT ACACTACCCC CTTTCCCAGC AGCTTCCTTT CGTTCCTCCA CTCCCTGTCC 
TGGGCCCTAA TTTTCCCTTG TTATTGGCTG GGAGATCGGT TACTGTCTTC ATTTTTGCCG 
ACCACCTATG AGAAACGACA ACGGTCAGAT GATCCATGTT ACTTCCAGGC CCTCTGCATT 
ATAATAACCA CACCCATTTA TTTAGCACTG CTGGTTTCAT CACTTCCGTT TGCCTTTATA 
GGATTTCTGG TGTGGTCGCC TATTCAGGCT GCACGAAGAC CCTATATCTA TTCCCATGTT 
CAGGAAAAAT CTCATGCAAC AGAAGCCAAG TTGCTGGCGG AATGGAGAGC AACAGGCAGT 
GGAAAAAGCT TCTGCTTTGG AACTGCCAAT GTTTGCCTCT TGCCTGACTC AATGGCAAGG 
TTCAATAACC TTTTTAATAC CCAAGCACGG GCTAAAGAAA TAGGGAGAAG AATACGCAAT 
GGGGCAAGTA GGCCCCAAAT TAAGATATAC ATAGACTCTC CTACTAACAC TTCTTTAAGT 
GCTGCCAGTT TTAGCAGTCT GGTGTCCCCA CAAGGTGGTG ATACATCTCA CCGAGCAGTA 
AATGTAGGTA TGAAGAGAAC AACATCTATG GAATACAAAG GGGAAAATTC TGGGGAAAAT 
AGCAGTGAGG AGGGCAGAGA TGGGAGAAAT GGAGGAGACA CAGCTGATGG AGAAGACAGT 
CCATGCATCA TTCGTATAGG TGGAGAGGAC AGTGGACACA TCTCTGACAC AGAGACTGAT 
GCTATTAGCG GGCATGTGAA TGTTGCATGC TTGGCAGAAA TTGAGCCAGA TTTTCAAAAA 
AGCCCAATTG CAAACCATAA GAATAAGGAT GCAGATGGTG GAAGTCTTGA TAGTTGTACA 
GCCTCTCGGG AGTCACTAGT CAAGGTCCGG GTGGCAGATG GAACTGCTGA CCAAAGCAGC 
ATTAACAATA AGCTTTTGTA TAAAACATCC ATAATCAAGA AGGCTACTGC CCGGAAGAAG 
AAACACACAG ATGACATTCT GGACCATGAG ATTTCTGCCT TTTTTCCTGC CAACCTGGAC 
TTTCTGTGTC TCCAGGAAGT ATTTGACAAA CGTTCTGCAA AGAAATTGAA AGAACAGTTG 
CACTACTATT TTGAATATGT GCTGTATGAT GTGGGGGTTT ATAGCTGCCA TGGGTGCTGT 
GGGTTTAAGT TCCTTAACAG TGGGCTTCTG TTTGCCAGTC GTTACCCCAT ACTAGATGCT 
ACCTATCACT GTTATCCCAA TGGGAGAGGA ATAGATGCAC TTGCTGCCAA AGGAGCTTTG 
TTTGTCAAGG TGCAAGTTGG AACCACTCCT CAGGACCAAA GAATTGTGGG ATACATCTCC 
TGCACTCACC TGCATGCTTT AGCAGGTGAT GCAATGACCC GATGTGAGCA ATTAGATATG 
CTCCAAGAAT GGGTGTCTGA ATTTCGCAAA TCTACCTCCT CCTCCAGTGC TGCAAATCCG 
GAGGAGCTGG TGGCTTTTGA CGTCATCTGT GGGGACCTTA ACTTTGACAA CTGCTCATCA 
GAAGACAAAC TGGAGCAGCA ACACTCTCTG TTTACACATT ACAAGGACCC ATGCCGGCTG 
AGCCCTGGGG AAGAAAAGCC CTGGGCAATA GGAACACTTT TGGACCCTGA GGGTCTGTAT 
GATGAGGAGG TGTGCACCCC AGATAATCTG CAGAAAGTCC TGGAGAATGA AGAAGGCAGG 
AAAGAATATT TAGTGTATCC TACCAGCAAG AACCATTGTC CCAACCAGAA GGGAAGAAAG 
GTTCCATTGA AAGGTGGTGG ACGCCGGGTG GATTATTTGT TATATACTGA GGAGGGACTC 
AACATGGAGT GGAAAGTGGA TGTAGAAGAA TACAGTTTTA TAACACAGCT AGCTGGACTT 
ACAGATCATG TGCCAGTAGC CATGCGACTG ACAGTTTCCA CAGGGGATGA AGAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913543.1
CDS	831..2810
Translation	MVLYTTPFPSSFLSFLHSLSWALIFPCYWLGDRLLSSFLPTTYEKRQRSDDPCYFQALCIIITTPIYLALLVSSLPFAFIGFLVWSPIQAARRPYIYSHVQEKSHATEAKLLAEWRATGSGKSFCFGTANVCLLPDSMARFNNLFNTQARAKEIGRRIRNGASRPQIKIYIDSPTNTSLSAASFSSLVSPQGGDTSHRAVNVGMKRTTSMEYKGENSGENSSEEGRDGRNGGDTADGEDSPCIIRIGGEDSGHISDTETDAISGHVNVACLAEIEPDFQKSPIANHKNKDADGGSLDSCTASRESLVKVRVADGTADQSSINNKLLYKTSIIKKATARKKKHTDDILDHEISAFFPANLDFLCLQEVFDKRSAKKLKEQLHYYFEYVLYDVGVYSCHGCCGFKFLNSGLLFASRYPILDATYHCYPNGRGIDALAAKGALFVKVQVGTTPQDQRIVGYISCTHLHALAGDAMTRCEQLDMLQEWVSEFRKSTSSSSAANPEELVAFDVICGDLNFDNCSSEDKLEQQHSLFTHYKDPCRLSPGEEKPWAIGTLLDPEGLYDEEVCTPDNLQKVLENEEGRKEYLVYPTSKNHCPNQKGRKVPLKGGGRRVDYLLYTEEGLNMEWKVDVEEYSFITQLAGLTDHVPVAMRLTVSTGDEES

Target ORF information:

RefSeq Version	XM_004913486.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sphingomyelin phosphodiesterase 3 (smpd3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913486.4

ORF Insert Sequence:

ATGGTTTTAT ACACTACCCC CTTTCCCAGC AGCTTCCTTT CGTTCCTCCA CTCCCTGTCC 
TGGGCCCTAA TTTTCCCTTG TTATTGGCTG GGAGATCGGT TACTGTCTTC ATTTTTGCCG 
ACCACCTATG AGAAACGACA ACGGTCAGAT GATCCATGTT ACTTCCAGGC CCTCTGCATT 
ATAATAACCA CACCCATTTA TTTAGCACTG CTGGTTTCAT CACTTCCGTT TGCCTTTATA 
GGATTTCTGG TGTGGTCGCC TATTCAGGCT GCACGAAGAC CCTATATCTA TTCCCATGTT 
CAGGAAAAAT CTCATGCAAC AGAAGCCAAG TTGCTGGCGG AATGGAGAGC AACAGGCAGT 
GGAAAAAGCT TCTGCTTTGG AACTGCCAAT GTTTGCCTCT TGCCTGACTC AATGGCAAGG 
TTCAATAACC TTTTTAATAC CCAAGCACGG GCTAAAGAAA TAGGGAGAAG AATACGCAAT 
GGGGCAAGTA GGCCCCAAAT TAAGATATAC ATAGACTCTC CTACTAACAC TTCTTTAAGT 
GCTGCCAGTT TTAGCAGTCT GGTGTCCCCA CAAGGTGGTG ATACATCTCA CCGAGCAGTA 
AATGTAGGTA TGAAGAGAAC AACATCTATG GAATACAAAG GGGAAAATTC TGGGGAAAAT 
AGCAGTGAGG AGGGCAGAGA TGGGAGAAAT GGAGGAGACA CAGCTGATGG AGAAGACAGT 
CCATGCATCA TTCGTATAGG TGGAGAGGAC AGTGGACACA TCTCTGACAC AGAGACTGAT 
GCTATTAGCG GGCATGTGAA TGTTGCATGC TTGGCAGAAA TTGAGCCAGA TTTTCAAAAA 
AGCCCAATTG CAAACCATAA GAATAAGGAT GCAGATGGTG GAAGTCTTGA TAGTTGTACA 
GCCTCTCGGG AGTCACTAGT CAAGGTCCGG GTGGCAGATG GAACTGCTGA CCAAAGCAGC 
ATTAACAATA AGCTTTTGTA TAAAACATCC ATAATCAAGA AGGCTACTGC CCGGAAGAAG 
AAACACACAG ATGACATTCT GGACCATGAG ATTTCTGCCT TTTTTCCTGC CAACCTGGAC 
TTTCTGTGTC TCCAGGAAGT ATTTGACAAA CGTTCTGCAA AGAAATTGAA AGAACAGTTG 
CACTACTATT TTGAATATGT GCTGTATGAT GTGGGGGTTT ATAGCTGCCA TGGGTGCTGT 
GGGTTTAAGT TCCTTAACAG TGGGCTTCTG TTTGCCAGTC GTTACCCCAT ACTAGATGCT 
ACCTATCACT GTTATCCCAA TGGGAGAGGA ATAGATGCAC TTGCTGCCAA AGGAGCTTTG 
TTTGTCAAGG TGCAAGTTGG AACCACTCCT CAGGACCAAA GAATTGTGGG ATACATCTCC 
TGCACTCACC TGCATGCTTT AGCAGGTGAT GCAATGACCC GATGTGAGCA ATTAGATATG 
CTCCAAGAAT GGGTGTCTGA ATTTCGCAAA TCTACCTCCT CCTCCAGTGC TGCAAATCCG 
GAGGAGCTGG TGGCTTTTGA CGTCATCTGT GGGGACCTTA ACTTTGACAA CTGCTCATCA 
GAAGACAAAC TGGAGCAGCA ACACTCTCTG TTTACACATT ACAAGGACCC ATGCCGGCTG 
AGCCCTGGGG AAGAAAAGCC CTGGGCAATA GGAACACTTT TGGACCCTGA GGGTCTGTAT 
GATGAGGAGG TGTGCACCCC AGATAATCTG CAGAAAGTCC TGGAGAATGA AGAAGGCAGG 
AAAGAATATT TAGTGTATCC TACCAGCAAG AACCATTGTC CCAACCAGAA GGGAAGAAAG 
GTTCCATTGA AAGGTGGTGG ACGCCGGGTG GATTATTTGT TATATACTGA GGAGGGACTC 
AACATGGAGT GGAAAGTGGA TGTAGAAGAA TACAGTTTTA TAACACAGCT AGCTGGACTT 
ACAGATCATG TGCCAGTAGC CATGCGACTG ACAGTTTCCA CAGGGGATGA AGAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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smpd3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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